LOCUS       DAOSDX010000001        12225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2004_length_12225_cov_2.870419, whole genome shotgun sequence.
ACCESSION   DAOSDX010000001 DAOSDX010000000
VERSION     DAOSDX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2004_length_12225_cov_2.870419"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLN02_00005"
     CDS             <1..965
                     /locus_tag="PLN02_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOI50734.1"
                     /translation="RSLASAGLHPGDRLHNAYGYGLFTGGLGLHYGAEELGVLTVPMS
                     GGQTQKQVMLLSDFEATALCCTPSYSLNIAEEAESIGIDLRKLPLRVGIFGAEPWTEE
                     MRYEIEARLGLEAIDIYGLSEVMGPGVGVECIEAKKGLHVWEDHFLVECVDVTTGLPV
                     PDGEEGEIVFTSLTKEAFPILRYRTRDISVIDRTPCKCGRTHARIRRISGRSDDMLII
                     RGVNVFPTQVEAVLMRTETLSPFYQIEVFREGFMDSMVVNVEASPPLFAKGQEAMDRI
                     AAKVQKDIKDFIGVSCQVIVKPTGAIERSMGKAVRVIDHRKKSG"
     gene            complement(957..1499)
                     /locus_tag="PLN02_00010"
     CDS             complement(957..1499)
                     /locus_tag="PLN02_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOI50735.1"
                     /translation="MRRLLKRYLPDHETIRRNPWLRPFENSLLHPRLWHLNRRSAAGA
                     VAAGLFCGLIPGPFQMPGAALCAILFRANLPLALVVTLYSNPFTIVPLYLLAYQTGRL
                     LIGDTAGFLLPPTFSFAEFGAWTEAMHAWMLAVARPLAIGLIVLADALALAGYLLTRG
                     AWRLYLVRHWHRRRRRTASA"
     gene            1560..3860
                     /locus_tag="PLN02_00015"
     CDS             1560..3860
                     /locus_tag="PLN02_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOI50736.1"
                     /translation="MKSAALAFALGVFLLQTQALLPDPLLWSLPALVLCLPAWRWRRW
                     PAQLLLLIGACGLGFGWAAWRAELRLADQLDAAWEGRDIAVVGVVAGLPQDFERGVRF
                     EFAIERRLDAEAIVPDRVQLAWYDDGATRHPVNPGERWQFAVRLKRPHGAANPGGFDY
                     ELWLLERGIRATGYVRPAPAQRLDAFVARPAYVVERLRAAVRQRFESALPAASHPWRG
                     ILTALAVGDQKAVDGELWTVFNRSGVTHAMAISGMHITLVAGLCGALLAGLWRRLPGL
                     ALRWPAQKVGVLTAALAAFGYTALAGFGVPAQRTLYMLLVAVLAMLSSRIVAPGRVLA
                     LALLVVLVVDPWAVLAPGFWLSFGVVAALLHVGAALVGAGRGWRERLRGWGTVQLAAT
                     LASLPILLLVFGQFPLVSPLANLLAIPVISLLVTPLALLAAVLPWTFLLQAAHAVLEP
                     LMQFLIWCVAWPLWQVPAPHPAAVLLAAIGVVVCLLPRGVPGRWLGLTLLLPLLFWPA
                     PEIPDGEARITVLDVGQGLAVTVRTRQHLLVYDTGPRYGSDADAGQRVVVPYLRWLGA
                     TRIDRLLLTHADADHAGGADSLRAALPVDESISSDAALGRRCAAGESWRWEGVDFAIQ
                     HPADEAYADNGRRNALSCVLAVTVGGERLLLTGDIGAREEAAMLARDAAALRSTVLLA
                     PHHGAKGSSTAEFLAAVAAREVLFSVGHRNRFGHPRPEVLERHAATGARLWRTDRDGA
                     LLLTLGPAGREIAAWRQLRQRYWQGR"
     gene            3888..4742
                     /locus_tag="PLN02_00020"
     CDS             3888..4742
                     /locus_tag="PLN02_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI50737.1"
                     /translation="MTIKQRSVRCLSPSGLHRMSYYEWGERDNPRVLVCVHGLTRNAR
                     DFDRLAQALSGHYRVVCPDVVGRGLSGWLRDPAAYAIPQYVADMVTLLARLDVETVHW
                     LGTSMGGLIGMALAAQENSPVMRLVLNDVGPSITRASLQRIAAYVGADPDWATPAEAL
                     AYLRQISAPFGALSEAQWLELTESSIRQRADGRWGFRYDPRIAEPFRAAFGDGDIALW
                     PLYEAIRCPTLAIRGADSDLLARDTWLRMGESGPRAELAEIPGVGHAPMFLSDDQIDV
                     VRNFLLHA"
     gene            4739..5497
                     /locus_tag="PLN02_00025"
     CDS             4739..5497
                     /locus_tag="PLN02_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI50738.1"
                     /translation="MKTVEIPGYRIETLDLAATRPGLPTLVLLHEGLGCVAMWRDFPQ
                     QLAAATGCRTIVYSRPGYGASDPHPEARQPDYMHREAQHILPALLAAMAVERPLLVGH
                     SDGGSIALIFAGSFPERPVGVVVMAPHEFVEEETLAGIRAARELWRDSDWPHKLARYH
                     RDAPRVFADWNDCWLAPAFRDWNIEDYLPAIRCPVLAIQGEDDEYATLRQIEVIAEQV
                     PETRLLKLARCGHTPQRDQPAAVLAAIAEFVSRR"
     gene            complement(5484..6080)
                     /locus_tag="PLN02_00030"
     CDS             complement(5484..6080)
                     /locus_tag="PLN02_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50739.1"
                     /translation="MSLYGDLSAPTPPSPEQFVESQPTAALPVPGAAEVGPPAVTAPT
                     EATAPLPTETLSAAPVVAVVTPAGETAVAAAPPVVADNSAQSRDELLRTLEAWRKAWA
                     NRDVEAYFSFYAADYAGNADSPAAWQASRRRIIEQAGLIDISFGEATIAMDGKDRATL
                     TFPMNYRSKRFEDEGVKQLQLVRDQGQWRIAREQFTAD"
     gene            complement(6261..6803)
                     /locus_tag="PLN02_00035"
     CDS             complement(6261..6803)
                     /locus_tag="PLN02_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOI50740.1"
                     /translation="MPIYQLGTKTPQLGEQSWIAPNATVIGDVRLGAQASIWWNATLR
                     GDNDPIHIGERSNIQDGSVLHTDEGVPMHIGNDVTVGHLVMLHGCTVGDGSLIGIGSV
                     ILNRAVIGSSSIVGANTLIPEGKIFPDGVLIVGSPGKVVRELSDEEISRLKHSASHYV
                     ENAKRYSQMLQAFPATIIQK"
     gene            6900..7334
                     /locus_tag="PLN02_00040"
     CDS             6900..7334
                     /locus_tag="PLN02_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50741.1"
                     /translation="MPFPKLKSGHWVLIVIALLVALDWAIRRPDGQTRQLNDVLSTQG
                     SPELQNYPYRFQVLRVEQGMAVLSTPRNVEVPAFRVLGVLHPEINVKDPNNPAFIAIE
                     KELARVQSEAAELVRAQPGIKGVRWELDRQWLRRQGIELMER"
     gene            7388..7744
                     /locus_tag="PLN02_00045"
     CDS             7388..7744
                     /locus_tag="PLN02_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOI50742.1"
                     /translation="MDDFSSLTAAVCEFRDARDWRQFHTLRNLIVSLNLEAGELLELM
                     QWKSDAEVEALPGEATTREALADECADVLLYLLLIAERSGIDLAAATRAKLQKNAAKY
                     PVDKARGSRAKYTELG"
     gene            7749..8927
                     /locus_tag="PLN02_00050"
     CDS             7749..8927
                     /locus_tag="PLN02_00050"
                     /inference="COORDINATES: protein motif:HMM:NF022738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HOI50743.1"
                     /translation="MADSGFYRDLPALPSFAAAINTRHHVDLPDDWWVVIADIVDSTR
                     AIADGAYKKVNTVGVACIAAIANLDRSIELPFLFGGDGATFAIPERLCAPAVIALRGA
                     QQLAADSFGLHLRAGLIRVGELRAAGHWVRVGKLALSEQINQPFFSGRGWEEAERRIK
                     RGDPGVQHVRLGEGPASASFEGFECRWQNVPSFRDVKLALIVVATALDPALNQATYAR
                     VLARIDEIFGDPGSHHPLHAGALQLSFSPLHLAGETRVRSAGRGFLASLAYACRLLLQ
                     NVAGWWLFRHDLDTASVAWSRYRDDLVRHSDFRKFDGALRMLLDAGEAQIAELAAWLD
                     AERAVGNLAWGMHRSREALITCLVESHAGKHLHFVDGSDGGYALAARQLKAQLGERRA
                     "
     gene            complement(8931..11288)
                     /locus_tag="PLN02_00055"
     CDS             complement(8931..11288)
                     /locus_tag="PLN02_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI50744.1"
                     /translation="MPERQPPSLRRISALVLGLLILILCAAGILLARQMLELQRLAVE
                     HRRQAAIGLSEKVRLVSNIERLILLGDQLSGSPEAESWQAAGRAFQALSRHPSLQGLV
                     ADDPAVADSFQVAERLLVLRESGATATDVEAAWENRRATLTRAAEETSGSMIAYSSSL
                     AETAAQSARQMLWQALAFCALLSFALIALLRLLKLQLIDPLLAVSGQLAAMRPNGQPA
                     APLPVARNLEVAEVFAALRALQDTQQALSDSEERQRLALAGANQGLYDLDMRSGRVRV
                     SPEYAEMLGYPPKTFADLDFTGWAALLHPDDREPTLQAYQDYVAGRTPRYRVEFRMRT
                     KDGQWKWLLSQASIQSRQADGTPLRMLGTHTDISAQKAAEARIRELNAELEARVSERT
                     AELDQARRVAECATLAKSAFLANMSHEIRTPLNAISGMAHLIRRAGLPDEQLARLDKL
                     EAASRHLLEIINDVLDLSKIEAGKLQLESSAFALPGLFENICSMLQERAEAKGIELRR
                     EYPADLRWLRGDSTRLQQALLNYAGNALKFTEHGSVLLRARVVDAQAAAVLVRFEVSD
                     TGIGIAPEAIDRLFNAFEQADHSTSRKYGGTGLGLAITRKIAEAMGGEAGADSLPGQG
                     SSFWFTAWLERGEALSPTTSDSIEQNEAGLAARHGMRHLLLVEDEPVNREIAQMLLLD
                     AGLAADLAEDGLQAVAMASTGHYDLILMDLQMPNLDGLEATRRIRRLPGYAAVPIVAM
                     TANAFVEDRERCRACGMNAFIAKPIVPDSFYEQLLQSLDAAYPGD"
     gene            complement(11295..12032)
                     /locus_tag="PLN02_00060"
     CDS             complement(11295..12032)
                     /locus_tag="PLN02_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOI50745.1"
                     /translation="MAATLLAMAASAGAPAEEVLQVAITDTAPPFAYRDDKGALKGFN
                     VDLVYALCQRLQRQCKIEVLRFPEVIPAVGGGRYDLGVANTLRTPEREKLVRFSRVIW
                     RSTSSLIGHRELGGSPPSALLKRETTCAIAGTRQFTWLGENTQTPPLAASGNQELLKL
                     LGQNQCALALMPTQQALVFLQGKEGQAFAYIGAPLAESGLGGDVHMVLRPGNDSLKAE
                     VDNALDALIRDGTHDRLSRRHFPYSIL"
BASE COUNT         1822 a         3949 c         4260 g         2194 t
ORIGIN      
        1 cccgtagcct ggcttccgcc ggcctgcatc ccggcgaccg cctgcacaac gcctacggtt
       61 acggcctgtt caccggcggc ctcggcctgc attacggcgc cgaggaactg ggcgtgctga
      121 ccgtgccgat gtccggtggc cagacccaga aacaggtcat gctgctctcc gacttcgaag
      181 ccaccgcact gtgctgcacg ccgtcctact cgctgaacat cgccgaggaa gccgagagca
      241 tcggcatcga cctgcgcaag ctgccgctgc gcgtcggtat tttcggcgcc gagccgtgga
      301 ccgaggaaat gcgctacgag atcgaggcgc gcctcgggct ggaagcaatc gacatctacg
      361 ggctgtccga agtcatgggg ccgggcgtcg gtgtcgagtg catcgaggcg aagaagggcc
      421 tgcacgtctg ggaagaccac ttcctcgtcg agtgtgtgga cgtgacgacc gggttgccag
      481 tgccggacgg tgaggagggc gagatcgtgt tcacctcgct gaccaaggaa gccttcccga
      541 tcctgcgtta ccgcacccgc gacatttcgg tgatcgaccg cacgccgtgc aagtgcggcc
      601 gcacccatgc gcgcatccgg cgcatctccg gacgttccga cgacatgctg atcatccgcg
      661 gggtcaatgt cttcccgact caggtcgagg cggtgctgat gcgtaccgaa accttgtcac
      721 ccttctacca gatcgaagtg ttccgcgaag gcttcatgga ttcgatggtg gtcaatgtcg
      781 aagccagccc gccgctgttc gccaagggcc aggaggcgat ggaccgcatt gccgccaagg
      841 tgcagaagga catcaaggac ttcatcggcg tttcctgcca ggtcatcgtc aaaccgaccg
      901 gcgccatcga gcgttcgatg ggtaaggcgg tgcgcgtcat cgaccaccgc aagaagtcag
      961 gctgaggcag tccgccggcg gcgccggtgc cagtggcgga cgagatagag ccgccaggcg
     1021 ccgcgcgtca gcaagtagcc ggccagcgcc agcgcatcgg caaggacgat caggccgatg
     1081 gcgagtggcc gggcgacggc cagcatccag gcatgcatgg cttccgtcca ggcgccgaac
     1141 tcggcaaagc tgaacgtcgg cggcaacagg aagccggccg tatcgccgat cagcaggcgg
     1201 ccggtctggt aggccagcag gtagagcggg acgatggtga acgggttgct gtagagcgtc
     1261 acgacgagcg ccagcggcag gttggcgcgg aacaggatgg cgcacaacgc ggcgccgggc
     1321 atctggaatg gcccgggaat cagcccgcag aacagcccgg cggcaacggc gccggccgcc
     1381 gagcggcggt tcaggtgcca cagccgcgga tgcagcagtg aattctcgaa cggccgcaac
     1441 caggggttgc ggcgtatcgt ttcgtggtcc ggcaggtaac gtttgagcag acggcgcatc
     1501 gccggattat cgcaaacggc gggctcgccg tcggtaacgc ttcgtgccat gatattggca
     1561 tgaaatccgc cgcgcttgct ttcgcgctgg gtgtgttcct gctgcagacc caggcgctgc
     1621 tgcccgatcc cttgctgtgg tcgctgccgg cgcttgtcct gtgcctgccg gcctggcgct
     1681 ggcgtcgctg gccggcgcaa ctgttgctgc tgatcggcgc ctgcggcctc ggtttcggct
     1741 gggcggcgtg gcgtgccgaa ctgcgcctgg ccgaccagct ggacgcggcg tgggagggca
     1801 gggacatcgc tgtggtcggc gtggttgcag gcctgccgca ggatttcgag cgcggtgtgc
     1861 gtttcgagtt tgccatcgag cgccgcctcg acgccgaagc catcgtcccc gaccgcgtgc
     1921 aactggcctg gtacgacgac ggcgcgacgc gccacccggt caatcccggt gaacgctggc
     1981 aattcgccgt ccgcctcaag cgaccgcacg gcgccgccaa tcccggtggt ttcgattacg
     2041 aactgtggct gctcgaacgc ggcatccgcg ccaccggcta cgtgcggccg gcgccggcgc
     2101 agcgtctcga cgctttcgtc gcgcgtccgg cctatgtcgt cgagcggctg cgggcggcgg
     2161 tccgccagcg tttcgaaagc gccctgccgg cggccagcca tccctggcgc ggcattctga
     2221 cggcgctcgc ggtgggcgac cagaaggcgg tcgacggcga gctatggacg gtcttcaacc
     2281 gcagcggcgt gacgcacgcg atggcgatca gcggcatgca catcaccctc gtcgccggtc
     2341 tctgtggtgc actgctggcc gggctgtggc gccgtttacc cggtctggcg ctgcgctggc
     2401 cggcgcagaa ggtcggcgtg ctgacggcgg cgctggcggc gttcgggtac accgcgctgg
     2461 ccggtttcgg ggtgccggcg caacgcaccc tgtacatgct gctggtcgcc gtcctggcca
     2521 tgctttccag ccgcatcgtc gcgcccggcc gggtgctggc gctggcgctg cttgtcgttc
     2581 tcgtcgtcga tccctgggcg gtgctggcgc cgggtttctg gttgtccttc ggcgttgtcg
     2641 ccgccttgct ccacgtcggc gcggcgctgg tcggcgccgg gcgaggctgg cgcgagcgcc
     2701 tgcgcggctg gggaacggtg caattggcgg cgacgctcgc ctcgttgccg atcctgctgc
     2761 tggtgttcgg ccagtttccg ctggtttcgc cgcttgccaa cctgctcgcg atcccggtga
     2821 tcagcctgct ggtgacgccg ctggcgctgc tcgccgccgt cctgccgtgg accttcctgc
     2881 tgcaggcggc gcacgccgtg ctcgagccgt tgatgcagtt cctcatttgg tgcgtggcct
     2941 ggcccttgtg gcaggtgccg gcgccgcatc cggcggcggt gctgctggcc gccatcggtg
     3001 tcgtcgtctg cctgttgccg cgtggcgtgc cggggcgctg gctggggctg accttgctct
     3061 tgccgctgct gttctggcca gccccggaaa ttcccgatgg cgaggcgcgc atcaccgtgc
     3121 tcgacgttgg ccagggcttg gcggtgacgg tacgaacccg ccagcacctg ctggtctacg
     3181 acaccggccc gcgctacggc agcgacgccg atgccgggca acgggtggtc gtgccctatc
     3241 tgcgctggct gggggcgacg cgcatcgacc gcctgctgct cacccacgcc gatgccgacc
     3301 atgctggcgg tgcagattcg ctgcgcgcgg cgctgccggt cgatgagagt atttcgtcgg
     3361 acgcggcgct ggggcggcgc tgcgccgctg gcgagagctg gcgctgggaa ggcgtcgatt
     3421 tcgcgatcca gcatccggcg gacgaggcct atgccgacaa cggccggcgc aatgcgctgt
     3481 cctgcgtgct ggcggtgacg gtcggcggcg aacgactgct gctcaccgga gacatcggcg
     3541 cccgcgagga ggcggcgatg ctggcacgcg atgctgcggc gctgcgcagc acggttctgc
     3601 tggcgccgca ccacggcgcc aagggctcgt cgacggcgga atttctcgca gcagtcgcag
     3661 cgcgggaggt gctgttttcg gtcggtcatc gcaaccgctt cggccatccg cgtccggagg
     3721 tgctggaacg ccacgcggcg actggcgccc ggctgtggcg caccgatcgg gacggtgcct
     3781 tgctgctgac gctaggtccg gcagggcggg aaattgccgc ctggcgacag ttgcgccaac
     3841 gttactggca gggacggtga tacattcctg ccgaggagga aggcgcaatg acgataaaac
     3901 aacgcagcgt ccgctgtctc agtccttcgg ggctgcaccg gatgagttat tacgaatggg
     3961 gcgagcgcga caatccgcgg gtgctggtct gtgtccatgg gctgacccgc aacgcgcgcg
     4021 atttcgatcg cctggcgcag gctttgtccg gccactaccg ggtcgtctgt ccggatgtcg
     4081 tcgggcgcgg cctcagcggc tggctgcgcg atcccgccgc ctatgcgatt ccccagtacg
     4141 ttgccgacat ggtcacgctg ctcgcccgcc tcgatgtcga gacggtgcat tggctgggca
     4201 cttcgatggg cggcctgatc ggcatggcgc tggcggcgca ggaaaattcg ccggtgatgc
     4261 gcctggtcct caacgatgtc gggccgtcga tcacccgtgc ttcgctgcag cgcatcgccg
     4321 cctatgtcgg cgccgatccg gactgggcca ccccggccga ggcgcttgcc tatctgcgcc
     4381 agatcagtgc gccgttcggc gcgctgagcg aggcgcaatg gcttgagctg accgaaagca
     4441 gcatccgcca gcgtgccgac ggtcgttggg gctttcgtta cgatccgcgc atcgccgagc
     4501 cgttccgggc cgccttcggc gatggcgaca tcgcgctgtg gccgctctac gaagcgatcc
     4561 gctgcccgac gctggccatc cgcggcgccg attccgacct gctggcgcgc gacacctggc
     4621 tgcgcatggg tgaatccggg ccgcgcgcgg aactggccga gattcccggg gtcggacacg
     4681 ccccgatgtt cctttccgac gaccagatcg acgtcgtccg caactttctc ctgcatgcat
     4741 gaagactgtc gaaattcccg gttatcgcat cgaaaccctt gatcttgccg ccacccgtcc
     4801 cggcctgccg accctggtcc tgctgcacga agggctgggc tgcgtcgcca tgtggcgcga
     4861 cttcccgcag cagctggcgg cggcgaccgg ttgccggacc atcgtctatt cgcgtcccgg
     4921 gtatggcgct tccgatccgc atccggaagc gcgccagccg gactacatgc accgcgaggc
     4981 gcaacacatc ctgccggctt tgctggcggc catggcggtc gagcggccgt tgctggtcgg
     5041 ccatagcgac ggcggcagca ttgcgctgat ctttgccggt agttttcccg aacggccggt
     5101 cggcgtggtg gtcatggcgc cgcacgagtt cgtcgaggag gaaaccctgg ccggcatccg
     5161 tgccgcgcgt gagctctggc gcgacagcga ctggccgcac aagctggcgc gctatcaccg
     5221 cgatgcgccg cgggtctttg ccgactggaa cgattgctgg ctggcgccgg cctttcgcga
     5281 ctggaacatc gaggattacc tgccggcgat ccgttgcccg gtgctggcga tccagggcga
     5341 ggacgacgaa tacgcgacgc tgcgccagat cgaggtgatt gccgagcagg tgccggaaac
     5401 acgcctgctc aagctggcgc gctgcggaca cacgccgcag cgcgaccagc cggccgccgt
     5461 gctggcggcg atcgccgaat tcgtcagtcg gcggtgaact gctcgcgggc gatccgccat
     5521 tgcccctggt cgcggaccag ttgcagctgc ttgacgcctt cgtcctcgaa gcgtttcgac
     5581 cggtaattca tcgggaaggt cagggtggcg cggtccttgc cgtccatcgc aatcgtcgct
     5641 tcgccgaagc tgatgtcgat caggccggcc tgctcgatga tgcggcgccg gctggcctgc
     5701 caggccgccg ggctgtcggc gttacccgcg tagtcggcgg cgtagaacga gaaataggct
     5761 tcgacgtcgc ggttggccca agccttgcgc caggcttcca gcgtgcgtaa cagttcgtcg
     5821 cggctttgcg ctgaattgtc ggcgaccacc ggcggcgctg cggcaacggc agtttcgccg
     5881 gccggggtga cgacggcgac gaccggagcg gcgctgagcg tttccgtcgg cagtggcgca
     5941 gttgcttccg tcggtgcagt taccgctggc ggccccactt cggccgcgcc gggcaccggc
     6001 aaggcggccg tcggctgact ttccacgaat tgttccggcg aaggcggcgt cggtgcgctg
     6061 aggtcaccgt aaaggctcat caatgcaatg gcgcacagag ctccgatcga gactgctgca
     6121 atcaggtaag tgctaaatcc cgtgttcccc ttgtccatgc tggctcccta tttatgttca
     6181 tgaatcgcaa cattattctg atcgacctgc gagaaacgat tctttcatga taaataagaa
     6241 gatttgtaaa gaaaggtgta ttacttttgt ataattgttg ctggaaacgc ttgcagcatc
     6301 tgggaatatc gtttggcatt ctcgacataa tggctagccg aatgttttaa acgggaaatt
     6361 tcctcgtcgg acagttcacg caccactttt cccggtgaac cgacgatcag cacgccatcc
     6421 gggaaaatct tgccttccgg gatcagggta ttggcgccga cgatgctcga actgccgatc
     6481 accgcccggt tgaggatgac cgaaccgatg ccgatcaggc tgccgtcgcc gaccgtgcaa
     6541 ccgtgcagca tgaccaggtg accgacggtg acgtcgttgc cgatatgcat cggcacgccc
     6601 tcgtcggtgt gcagcaccga accgtcctgg atgttgctgc gctcgccgat gtggatcggg
     6661 tcgttgtcgc cgcgcaaggt ggcgttccac cagatcgagg cctgggcgcc gaggcggacg
     6721 tcgccgatga ccgtggcgtt gggggcgatc cagctctgct cgccgagttg cggggttttt
     6781 gtaccgagtt gatagatggg catgggtttt ctccgtgtag ggcaggtgtg ccgagcgatt
     6841 ttctcatgtt acgcttcgcc accgagcggg ctgcggggcc ggccaagcaa agagacagca
     6901 tgccatttcc gaagctcaaa tccggacact gggtcctcat cgtcatcgcc ctgctggtcg
     6961 ccctcgactg ggcgatccgc cggccagacg gccagacccg ccagctcaac gacgtcctgt
     7021 ccacgcaggg cagcccggaa ctgcagaact atccctaccg tttccaggtg ttgcgcgtcg
     7081 agcaggggat ggcggtgctg agcacgccgc gcaatgtcga ggtgccggcg ttccgggtac
     7141 tcggcgtgct gcacccggaa atcaatgtca aggatccgaa caatccggcc ttcatcgcca
     7201 tcgagaagga actggcccgg gtgcagtccg aagccgccga gctggtccgg gcacagcccg
     7261 ggatcaaggg ggtgcgctgg gaactcgacc gtcagtggtt gcgtcgccag ggtatcgagc
     7321 tgatggagcg ctgaagggct gggctgtggc atgatgccgc gcttggcagg cttaaacggg
     7381 acgccgaatg gacgactttt ccagtttgac cgccgccgtg tgcgaatttc gtgatgcgcg
     7441 cgactggcgg cagtttcata ccctgcgcaa cctgatcgtc tcgctcaacc tggaagccgg
     7501 cgagctgctc gaactgatgc agtggaagag cgacgccgag gtcgaggcgc tgccgggcga
     7561 ggcgacgacg cgcgaggcgc tggccgacga atgcgccgac gtgctgctct acctgctgct
     7621 gatcgccgag cgcagcggca tcgacctggc ggcggcgacc cgcgccaagc tgcagaagaa
     7681 cgccgccaag tacccggtcg acaaggcgcg cggctcgcgc gccaaataca ccgaactcgg
     7741 ctgaggcgat ggccgattcc ggtttctacc gtgacctgcc ggccctgccg tcgttcgcgg
     7801 cggcgatcaa tacgcgccat cacgtcgatc tgcccgacga ctggtgggtg gtgatcgccg
     7861 acatcgtcga ttcgacccgg gcgatcgccg acggcgccta caagaaggtc aataccgtcg
     7921 gcgtcgcctg catcgcggcg atcgccaacc tcgaccgcag catcgaactg cctttcctgt
     7981 tcggtggcga cggtgccacc ttcgccattc ccgaacgtct ctgcgcaccg gcggtgatcg
     8041 ccttgcgcgg tgcccagcaa ctggcggccg acagtttcgg cctgcacttg cgcgccggcc
     8101 tgatccgcgt cggcgaactg cgcgcggccg ggcactgggt gcgggtgggc aagctggcgt
     8161 tgtcggaaca gatcaaccag cccttctttt ccgggcgtgg ctgggaggag gccgagcgcc
     8221 ggatcaagcg cggcgatccc ggcgtgcagc acgtccgtct gggcgagggg ccggccagcg
     8281 ccagcttcga gggtttcgag tgccgctggc agaacgtgcc gagtttccgc gacgtcaagc
     8341 tggcgctgat cgtcgtcgcc acggcgctcg atccggcgct caaccaggcg acctatgccc
     8401 gggtgctggc gcggatcgac gagattttcg gcgaccccgg cagccatcat ccgctgcatg
     8461 ccggggcgct gcagttgtcc ttctcgccgc tacatctcgc cggcgagacc agggtgcgca
     8521 gcgccgggcg cggttttctt gccagtctcg cctatgcctg ccgcctgctg ctgcagaatg
     8581 tggcgggctg gtggctgttc cgccacgacc tcgacacggc gtcggtcgcc tggagccgct
     8641 accgcgacga tctggtccgg cacagcgatt tccgcaagtt cgacggcgcc ctgcgcatgc
     8701 tgctcgacgc cggcgaggcg cagatcgccg aactcgccgc ctggctcgac gccgagcggg
     8761 cggttggaaa cctggcctgg ggcatgcacc gctcgcgtga ggcgctgatc acctgcctgg
     8821 tcgaatcgca cgccggcaag cacctgcatt tcgtcgacgg cagcgacggc ggttatgcgc
     8881 tggcggcgcg tcagctcaag gcgcaactcg gcgagcgccg cgcctgaagc tcagtcgccg
     8941 gggtaggcgg cgtccaggct ctgcagcaac tgttcgtaga agctgtcggg gacgatcggc
     9001 ttggcgatga aggcgttcat gccgcaggcg cggcagcgct cgcggtcctc gacgaaggcg
     9061 ttggcagtca tggcgacgat cggtacggca gcgtagccgg gcaggcggcg gatgcgccgg
     9121 gtggcttcga ggccgtcgag attcggcatc tgcaggtcca tcaggatcag gtcgtaatgg
     9181 ccggtgctgg ccatcgccac cgcctgcagg ccatcctcgg cgaggtcggc ggcgagcccg
     9241 gcgtcgagca gcagcatctg ggcgatctcg cgattgaccg gttcgtcctc gaccagcagc
     9301 aggtggcgca tgccgtgccg tgccgcgagg ccggcctcgt tctgctcgat gctgtcgctc
     9361 gtggtcgggg acagcgcttc gccgcgttcc agccaggccg tgaaccagaa gctgctgccc
     9421 tggcccggca ggctgtcggc gccggcttcg ccgcccatcg cctcggcgat cttgcgggtg
     9481 atcgccagcc ccaacccggt accgccgtac ttgcgcgaag tcgaatggtc ggcctgttcg
     9541 aaggcattga acaggcggtc gatcgcttcc ggcgcaatgc cgatgccggt gtcgctgact
     9601 tcgaaacgga ccagcaccgc cgccgcctgg gcgtcgacga cccgggcgcg cagcaggacg
     9661 ctgccgtgct cggtgaattt gagcgcgttg cctgcgtagt tgagcagcgc ctgttgcaga
     9721 cgcgtgctgt cgccgcgcag ccagcgcagg tcggccgggt attcgcggcg cagttcgatg
     9781 cccttggctt cggcgcgttc ctgcagcatc gagcagatgt tctcgaacag gccgggcagg
     9841 gcgaatgcgc tgctttccag ttgcagcttg ccggcttcga tcttcgacag gtcgagcacg
     9901 tcgttgatga tttccaggag gtgacggctg gccgcttcca gcttgtcgag acgggccagt
     9961 tgctcatcgg gtagtccggc gcggcggatc aggtgggcca tcccggagat ggcgttgagc
    10021 ggggtgcgga tttcgtggct catgttggcc aggaaggcgc tcttggccag cgtcgcgcac
    10081 tcggcgacgc gccttgcctg gtcgagttcg gcggtacgtt cgctgacgcg cgcctcgagc
    10141 tccgcgttca gttcgcggat gcgggcttcg gcggctttct gggcggagat gtcggtgtgc
    10201 gtgccgagca tgcgcagcgg cgtgccgtca gcctggcgcg actggatgct ggcttgcgag
    10261 agcagccatt tccactggcc gtccttggtc cgcatgcgga attcgacccg gtaccgggga
    10321 gtccggccag ccacgtaatc ctggtaggcc tgcagtgtcg gttcccggtc gtccggatgg
    10381 agaagcgccg cccagccggt gaaatcgagg tcggcaaagg tcttgggcgg atagccgagc
    10441 atttccgcat attcggggct gacccggact cggccgctgc gcatgtcaag gtcgtagagg
    10501 ccctggttgg cgccggccag cgccagtcgc tggcgctcct cgctgtcgct gagcgcctgc
    10561 tgggtatcct ggagcgcccg cagcgcggcg aagacttcgg ccacctcgag gttgcgcgcc
    10621 accggcaagg gagcggccgg ctggccgttc gggcgcatcg ccgcgagctg accggagacc
    10681 gccagcaagg ggtcgatcaa ctgcagcttg agcagacgca gcagggcgat cagggcaaac
    10741 gagagcagcg cgcagaaggc cagcgcctgc cacagcatct gccgcgccga ctgggcggcg
    10801 gtctcggcga ggctgctgct gtaggcgatc atgctgcccg aggtttcctc ggcggcgcgg
    10861 gtcagcgttg cccggcggtt ttcccaggcg gcctcgacat ccgtcgccgt ggcgccgctt
    10921 tcgcgcagga cgagcaggcg ttcggcgacc tggaaactgt cggcgaccgc cggatcgtca
    10981 gcgaccaggc cttgcagcga tggatgccgg ctgagcgcct ggaaggcacg gccggcggcc
    11041 tgccagcttt ccgcctccgg gctgccgctc aactggtcgc cgagcaggat caggcgctcg
    11101 atgttgctga ccaggcgcac cttttcgctc agtccgatgg cggcctgccg acgatgctcc
    11161 accgccaggc gttgcaattc cagcatctgc cgcgccagca ggatgccggc ggcgcacagg
    11221 atcaggatca gcagccccag gacgagcgcc gagatgcggc gcaggctggg gggctggcgt
    11281 tcgggcatgg gcgcttacag gatgctgtag ggaaaatgtc gccgcgacag gcgatcgtgg
    11341 gtgccatcgc ggatcagggc gtcgagcgcg ttgtcgacct cggccttgag gctgtcgttg
    11401 ccggggcgca ggaccatatg gacatcgccg ccgaggccgc tttcggcgag tggggcgccg
    11461 atgtaggcga aggcctggcc ttccttgccc tgcaggaaaa ccagtgcctg ctgcgtcggc
    11521 atcagggcga gcgcgcactg gttctggccg agcagcttga gcagttcctg gttgccgctg
    11581 gcggccagcg gcggcgtctg ggtattctcg ccgagccagg tgaactggcg ggtgccggcg
    11641 atggcgcagg tggtttcccg cttcagcagc gcgctcggcg gcgagccgcc gagttcgcgg
    11701 tggccgatca ggctcgacgt cgagcgccag atgacgcggc tgaaacggac cagtttttcg
    11761 cgttccggcg tgcgcaaggt gttggcgacg ccgaggtcgt agcggccgcc gccgacggcg
    11821 ggaatcactt ccgggaaacg cagcacctcg atcttgcatt gacgctgcag gcgctggcac
    11881 agcgcgtaca ccagatcgac gttgaacccc ttcagggcac ccttgtcgtc acgataggcg
    11941 aagggtggcg cggtatcggt gattgccacc tgcagcactt cctcggccgg ggcgccggca
    12001 ctggcagcca tcgccagcag cgttgctgcc atccgcacaa tccgtcccgc cacgacctca
    12061 cccctgagcg ctgattagaa acgcggctga ctttattact taagtaatag atgtgcaagc
    12121 aaacgatcta tgctgtttcg gtcgttcgct aaaccgactg gctttccagt gtgtgccggg
    12181 cgccgtcgcc ctgggcaagt agcgaagcca gcgtcggcag ggttt
//
LOCUS       DAOSDX010000002        11280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2212_length_11280_cov_3.525880, whole genome shotgun sequence.
ACCESSION   DAOSDX010000002 DAOSDX010000000
VERSION     DAOSDX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_11280_cov_3.525880"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLN02_00065"
     CDS             <1..483
                     /locus_tag="PLN02_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI50746.1"
                     /translation="PRRGATVREVGADRVCEMLEVMAELEAMCGRLAARRITADEQLA
                     LQEAHRACEAARDANTPDVYYQLNEVFHQRIYEASHNSFLVEQATALHRRLRPYRRLQ
                     LRVRNRMATSFNEHQAIVDAIVKGDSDLASAQLRAHVTVQGERFADLVATLRDFKAGG
                     "
     gene            complement(490..1290)
                     /locus_tag="PLN02_00070"
     CDS             complement(490..1290)
                     /locus_tag="PLN02_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOI50747.1"
                     /translation="MTLWWLTYPLLGIFGGFVAGLFGVGGGLTLVPFMYMLFVAQNFP
                     PEHLMHLALGTSMATIVFTSISSMRAHHSHGAVRWDIVRTMAPGLVLGTFGGSLVAGQ
                     VPTGPMTAIFVVIVYYASAQMMLDFKPHAHRQMPNALGRFVAGSVIGVISSLVAAGGG
                     FLSVPFMLFCNVAIHNAVGTSSALGFPIAVAGAVGYIVAGLNDSGLPAYTLGYIYLPA
                     FFGIVVMSISMAPVGARLAHKLPVKQLKRAFGAFLALLATKMLHSLIG"
     gene            complement(1287..1739)
                     /locus_tag="PLN02_00075"
     CDS             complement(1287..1739)
                     /locus_tag="PLN02_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOI50748.1"
                     /translation="MKSEPDEVSIDDLAAAPQRAFPWTGVRNYQARNFMRDAMQPGDL
                     AFFYHSGCKEPGIAGICEICSDSYPDPTQFSATDKYHDPKSTPEQPRWWLRDVRFVSR
                     SRLLGLGELRRHPELAGMRVLARGNRLSITPVTEAEWNFIDRLLETDT"
     gene            complement(1808..1987)
                     /gene="ssrS"
                     /locus_tag="PLN02_00080"
     ncRNA           complement(1808..1987)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLN02_00080"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2012..2338)
                     /locus_tag="PLN02_00085"
     CDS             complement(2012..2338)
                     /locus_tag="PLN02_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOI50749.1"
                     /translation="MSGEQNFLDVKIMGREYRVACLPEERDALLAAVDMVDGKMRDIA
                     QRTRSTIAERVAVMAALNIAHEHLGQPVPDDNQETVASPETKRRIEAMGARLDAVLAP
                     QQQLDF"
     gene            complement(2338..2535)
                     /locus_tag="PLN02_00090"
     CDS             complement(2338..2535)
                     /locus_tag="PLN02_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50750.1"
                     /translation="MNSELEALETKIDQVVALVHQLRAENEVLKNQIAAAEAERLHLR
                     STMTAARERLEGLIEQIPEDA"
     regulatory      2649..2854
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2959..4788
                     /locus_tag="PLN02_00095"
     CDS             2959..4788
                     /locus_tag="PLN02_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI50751.1"
                     /translation="MSIRFRPLLGLVPAACIAVAGAAETVKLDDVVVTATRQAMRADE
                     VLTDTTVLERAEIEAAGHSSLEQILAAQPGIQMAANGSPGAASYLYVRGANPKHVLLL
                     IDGMRVGSATSGEPTWSRIPAAQIERIEIVRGSASSLYGSDAIGGVVQVFTRRGSGPL
                     KFSATAGGGSDSARSASAGLAGSQGSWRYALNLSEDASDGFNSKPKDPKANPDRDGFR
                     NRAAAGSLGYQFAPGHELGLTFLHAEGRSRYDGSGAKVNWVNSTRNGAASLVLQNALS
                     DQWTSRLSVGRSIDDSSNLKDGIRNSRFNTEQRQYAWQHDIQGRLGNFLVGVERLEQE
                     IDTSGNYARDERRNDAVQLGWNRGFGAHRLQASVRHDDDSLFGDKTSGSLAYGYRFSP
                     NWRASLSAGTAFKAPTFNELYYPASGSYAGNPDLRPESSRNREAALHYENGSQHVSLT
                     WYLNRIEDMIVWTKVGALNQPTNVENARIEGTTLAWQGRLAGFDLQASHDWLDAEDRS
                     NHRQLPRRARHTVAAAVARSVGDWDWRFGLTASGKRYEYKSNARVGLSGYTLANLYAA
                     YRFSPDWAVFARINNLFDRDYTLADGYATAGRNAFIGIRYSPK"
     gene            4795..5772
                     /locus_tag="PLN02_00100"
     CDS             4795..5772
                     /locus_tag="PLN02_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOI50752.1"
                     /translation="MPTRRRAFLILASLALLSLASFALALAAGSLRVAPAEIVAALFG
                     QGDGTGAMVVQLRLPRALAGFACGGLLALAGALMQVLLRNPLADPYVLGISGGAGVGA
                     MFAMLLGLPLLAVDGLAFAGAFGAMFLVFGLAHGDGSWTQTRLLLTGVIVAAGCGALI
                     ALMLSIAEESKLHGMLFWLMGDLGQTYTWWPTLLVLILALAVAMPFARELNLLSRGLM
                     QAQALGVAIDRLRVAIYLLASLATAASVTTAGSIGFVGLVVPHLVRLALGNDQRLLLP
                     AAVLAGGSLLVFADTAARTVIAPQQLPVGVLTALIGVPVFLFLLSRQPK"
     gene            5769..6542
                     /locus_tag="PLN02_00105"
     CDS             5769..6542
                     /locus_tag="PLN02_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI50753.1"
                     /translation="MSAPLLEARQLGVDIGGKTVVDRLNLSLQPGESLAILGRNGAGK
                     STLLSTLAGLRPPRAGAVLLDGEDCALLPPRQAALRRGWLGQFQSDPFGSTVLETALT
                     GRHPHLGRWDWESKRDAELARGALKALGLAGMEARQVHTLSGGERQRLSIATLLTQAP
                     PLYLLDEPLSHLDLNHQMAVLELFASAARDCGAAVVMVLHDPALAHRFCDRALLVYGD
                     GRCAQGTVDTVLTAATLSGLYGYGLRQIEDHGQRCFIPE"
     gene            6547..7137
                     /gene="cobO"
                     /locus_tag="PLN02_00110"
     CDS             6547..7137
                     /gene="cobO"
                     /locus_tag="PLN02_00110"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOI50754.1"
                     /translation="MTISHEQRMQKKKAVIDGKIDAAQQERGVLVINTGNGKGKSSAA
                     FGVVARALGHGFKVGVVQFVKGRSDTGEEAFFRQQPNVAWHVGGEGFTWETQDKERDA
                     RAAQAAWDVACQHLADPDIGLVVLDEMTYAFKYGWLDLDAVIARLLSRPALQHVIVTG
                     RAAPQALRDAADTVSEIGMEKHAFQAGVKAMPGLEY"
     gene            7137..8417
                     /locus_tag="PLN02_00115"
     CDS             7137..8417
                     /locus_tag="PLN02_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOI50755.1"
                     /translation="MPACPALLVAAPASGQGKTTVTAALARLHARQGRRVTVFKCGPD
                     FLDPQIHAFASGRPCQNLDLGMCGEDDARWRLARAAAESDLILVEGVMGLFDGRPSAA
                     YIAERFAIPVMALIDAGAMAQTFGAVAHGLATYRPALPFGGVLANRVASPRHAAMLQE
                     SLKPGMGWFGALPKSGDSLPERHLGLLQAAEIDDLASRLDKLADALAASATVDLPKAV
                     NFTAAPPPALPPLLAGRRIAIARDAAYGFIYPANLETLFGLGAELRFFSPLAGDPLPE
                     CDAVWLPGGYPELHATALSANVGLWQALHAHVDAGKPLLAECGGMMSLFEEVVDKAGQ
                     TYRFAGLLPGRAVMQPRLAALGTQFADLPEGRLAGHTFHYSKSETPLSPLCRAVTAEG
                     REGEAIFRRQRLTASYVHFYFPSQPQAAAALFGA"
     gene            8516..9388
                     /locus_tag="PLN02_00120"
     CDS             8516..9388
                     /locus_tag="PLN02_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HOI50756.1"
                     /translation="MNKFIAMAAAVILGLTLNINDAEAKRLGGGSSFGMQRQATPPSR
                     NVQQAPHQAPAAAPQAQPKRSWMGPIAGLAAGLGLAALASHFGFGEGLANFMMIALLA
                     FAVIAAIGFFMRKKAGAQAQGMQYAGAGANYGGQPGNVARPDFTPAGGSSFTSAAQAV
                     ESTGGNIPADFDVEAFVRNAKVNFIRLQAANDAGNLDDIREFTAPEMFAEIKMAIEER
                     KGASQETDVVHLDAQVIDVVEEASRYIVSVHFSGQIREERNGPVENFSEIWHMTKPLS
                     GNRGWVIAGIQQVQ"
     gene            complement(9447..10196)
                     /gene="cobA"
                     /locus_tag="PLN02_00125"
     CDS             complement(9447..10196)
                     /gene="cobA"
                     /locus_tag="PLN02_00125"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOI50757.1"
                     /translation="MLSRGSEFPAGRVALIGAGPGDPELLTLKAYRRLQQAEVMLYDN
                     LVSPEIVDFCPSTVERIYVGKSAGNHTLPQEEISRRLLDLARASRRVVRLKGGDPFVF
                     GRGGEEMALLVEHGIEVEIVPGITAALGAAAAFGFPLTHRDHAQSCVFVTGHYKDHSI
                     DLNWQALAQPQQTIAIYMGVKGLDIIARQLQAHGLPGTTPAALIYRATWPNQKIYPAT
                     LATLPEVARRHAVAPPALLVIGDVVALAENP"
     gene            complement(10190..10564)
                     /locus_tag="PLN02_00130"
     CDS             complement(10190..10564)
                     /locus_tag="PLN02_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOI50758.1"
                     /translation="MKAEHDESEIIKPKIRDYSRQILVCTGPRCTQNGEGEALFNALQ
                     GKLRAAGLGVDAGPARVKRTRTGCVAACQGGPIVAVQPDGVWYYNISDANIDRIVEQH
                     LKGGAVVEDLVFHRNGGGDEAC"
     gene            complement(10554..>11280)
                     /locus_tag="PLN02_00135"
     CDS             complement(10554..>11280)
                     /locus_tag="PLN02_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HOI50759.1"
                     /translation="SSGDAGVYGMAGPTFEVLFQAGWTPDADIEVEIVPGASAINTCA
                     ALVGAPLTHDFCAISLSDLLTPWPTIARRLDAVAYADFVVALYNPKSGRRTQQIVEAQ
                     RLFLRHRDPQTPVAIVKSGHRPKQRIELTTLERMAEAEIGMLSTVLIGNSNTFIKHGL
                     MVTPRGYANKYAVEAGERNTHAGEQAGRSLSTGLNGWLATIQASGRSAAELAADYGLP
                     EDYIAAALQSELPPENEEAEIEG"
BASE COUNT         1851 a         3789 c         3701 g         1939 t
ORIGIN      
        1 ccacggcgcg gcgccaccgt tcgggaggtg ggggccgatc gggtctgcga gatgttagag
       61 gtgatggccg agctggaggc gatgtgcggg cgtctggctg cccggcggat caccgcggac
      121 gagcaactgg ccttgcagga ggcacatcgt gcctgcgaag cggcgcgcga tgccaacacg
      181 ccggatgtct attaccagtt gaacgaggtg tttcaccagc gtatctacga agccagccac
      241 aatagttttc tggtcgagca ggcgacggcc ctgcaccgtc gcctgcgtcc ctaccggcgg
      301 ctgcaattgc gcgtcaggaa ccgcatggcg acatcattca acgagcatca ggcgatcgtc
      361 gacgccatcg tcaagggcga cagcgacctc gcttcggcgc agttgcgagc ccacgtgacg
      421 gtgcagggtg agcgctttgc cgatctcgtc gccaccttgc gcgatttcaa ggctggcggt
      481 tgagccgttt cagccgatca ggctgtgcag catcttggtg gcaagcaagg cgagaaaggc
      541 gccgaaagcc cgcttcagct gtttgaccgg tagtttgtgc gccagtctgg cgccaactgg
      601 cgccatcgat atgctcatca cgacgatgcc gaaaaacgcc ggcaggtaga tatagcccag
      661 cgtataggcc ggcaatccgc tatcgttcaa accggcaacg atatagccga ccgctccggc
      721 gacggcaatc ggaaaaccca acgccgatga agtgccgaca gcattgtgga tggcgacgtt
      781 gcagaacagc atgaacggca ccgaaaggaa gcctccgcca gcggcgacca ggctggaaat
      841 gacgccgatg acgctgccgg cgacgaaacg tcccagggca ttcggcatct ggcgatgggc
      901 gtgcggcttg aagtcgagca tcatctgggc tgatgcgtaa tagacgatga cgacgaaaat
      961 cgccgtcatc ggtccggtcg gtacctggcc ggcgaccagc gaaccgccga aggtgccgag
     1021 caccaatccc ggtgccatcg tccgaactat gtcccagcgc acggcgccgt gggaatgatg
     1081 agcgcgcatg ctggaaatcg aagtgaatac gatggtcgcc atcgaggtgc cgagcgccag
     1141 atgcatcagg tgctcgggcg ggaagttctg ggcgacgaac agcatgtaca tgaacggtac
     1201 cagcgtcagg ccgccaccaa caccgaacag accggcgaca aaaccgccga aaattcccag
     1261 cagagggtaa gtgagccacc aaagcgtcat gtgtcagttt ccagcaaacg gtcaatgaaa
     1321 ttccactccg cctctgtcac cggcgtgatc gacagccggt tgccgcgcgc cagcacgcgc
     1381 atgccggcca gttccggatg gcggcgcagc tcgccgagac cgagcagccg ggagcggctg
     1441 acgaaacgca cgtcgcgcag ccaccagcgc ggctgttcgg gtgtcgattt cgggtcgtga
     1501 tacttgtcgg tggcggaaaa ctgggtcggg tccggatagc tgtccgagca gatttcgcag
     1561 atgccggcga tgcccggttc tttacagccg gaatggtaga agaaggcgag gtcgccgggc
     1621 tgcatggcgt cgcgcatgaa gttgcgcgcc tggtagttgc gcaccccggt ccacggaaag
     1681 gcgcgttgcg gcgcggccgc caggtcgtcg atcgaaacct cgtccggctc cgatttcatc
     1741 agccagtact gcatcttccg gtcacaaaat gaaaacaggt ggcagaccag ctgccacctg
     1801 tcaaaaaaaa gcccccgcga gtgccgtcag gccggcatcc tgaacctggg ttcagagtgg
     1861 ctgctttcct tccgcatcag gcacgttcga cgaagaacgc gcacaacagc gggtttctgg
     1921 tccgcaaccg atgccaatca gcattggttc aaggaatata tggccttaac aaacaccgca
     1981 ggggacactc ggcgggccac cccgcaaccg gtcagaagtc cagttgctgc tggggtgcca
     2041 gcacggcatc caaccgtgct cccatggctt cgattctacg cttcgtttcc ggcgacgcaa
     2101 ccgtttcttg gttgtcgtcc ggcacgggct ggcccaggtg ctcgtgggca atgttgagcg
     2161 ctgccatcac cgcgacgcgt tcggcgatcg tgctgcgcgt gcgctgggcg atgtcgcgca
     2221 tcttgccgtc gaccatgtcg accgccgcca gcagggcatc acgttcttcg ggcaggcagg
     2281 caacgcggta ctcgcggccc atgatcttga cgtcgagaaa attctgctcg ccgctcatca
     2341 ggcatcctcg ggaatctgtt cgatcaggcc ttcgaggcgt tcgcgggcgg ccgtcatggt
     2401 cgagcgcaaa tgcaggcgtt ccgcttcggc agcggcgatc tggtttttca agacttcgtt
     2461 ttcggcgcgc aactggtgca ccagggcgac gacctgatcg atcttggttt cgagcgcttc
     2521 gagttcgcta ttcatgggcc gcactataga gggataaatg tagtctggtc aaggactaag
     2581 cggctatttt caaagtgatt tatcgctttt gcgcagaggc cggctcgcgc tgcttgaagc
     2641 ggcgatgtag aatgccccgc ttgtcaggtg ccgtaagcga attttattcc atacggttaa
     2701 acgggaaagc ggtgcgtctc tccgagacca agccgctgct gcccccgcaa cggtaagcaa
     2761 gtgcgggcgc atcacgaagc cactggggaa acctgggaag gcgatgcgtc aagacttgcg
     2821 agcccggata ccggcctgcc atgcctcacg gaaatgccac ggggtggtgg cgccggtttc
     2881 ccgacctgcg ccctgcccgt gcgtttcctt ttcaacgcat tccggcatgc ggggacgcct
     2941 gccgggaaag ggaaaaccat gtccatccgt ttcaggcccc tgctgggctt ggtgccggcc
     3001 gcttgcatcg cagtcgctgg cgccgccgaa accgtcaagc tcgacgatgt cgtcgtcacc
     3061 gccacccgtc aggccatgcg cgccgacgaa gtcctcaccg atacgaccgt cctcgaacgc
     3121 gccgagatcg aggctgccgg ccattccagc ctggaacaga tcctcgccgc gcaacccggc
     3181 atccagatgg cggccaacgg cagcccgggc gcagcgtcct acttgtacgt ccgcggcgcc
     3241 aatcccaaac acgtcctgtt gctgatcgac ggcatgcgcg tcggctcggc gacttccggc
     3301 gagccgacct ggtcgcgcat cccggcggcg cagatcgagc gcatcgagat cgtccgcggg
     3361 tcggcgtcgt cgctctacgg cagcgatgcg atcggcggcg tggtccaggt atttacccgg
     3421 cgtggcagcg ggccgctgaa gttttcagct accgctggcg gcggtagcga tagcgcccgc
     3481 tcggccagcg ccgggctggc cggcagccag ggtagctggc gctacgcgct gaacctgagc
     3541 gaagacgcga gcgacggctt caacagcaag ccgaaggacc cgaaagccaa tcccgaccgc
     3601 gacggcttcc gcaaccgggc ggcggccggc agcctgggtt accagttcgc gcccggccac
     3661 gaactcggcc tgactttcct gcatgccgag gggcgcagcc gctatgacgg ttccggcgcc
     3721 aaggtgaact gggtcaacag cacgcgcaac ggcgcggcca gcctggtctt gcagaacgcc
     3781 ctgagcgatc aatggaccag tcgcctgagc gtcggtcgca gcatcgacga ttcgagcaac
     3841 ctcaaggacg gcatccgtaa cagccgtttc aacaccgaac agcggcaata cgcctggcag
     3901 cacgacatcc agggccgcct cggcaatttc ctggtcggcg tcgagcgtct cgaacaggaa
     3961 atcgacacca gcggcaacta tgcccgcgac gaacgccgca acgatgccgt gcagctcggc
     4021 tggaatcgcg gcttcggtgc gcaccggctg caggcgtcgg ttcgccatga cgacgattcg
     4081 ctgttcggtg acaagacttc cggctcgctc gcctatggct accgcttctc gccgaactgg
     4141 cgggccagcc tgagtgccgg tacggctttc aaggcgccga ccttcaacga actctactat
     4201 ccggcgagcg gcagctatgc cggcaacccg gacctgcgcc cggagtcgtc gcgcaaccgg
     4261 gaagcggcgt tgcattacga gaatggcagc cagcacgtct cgctgacctg gtacctcaac
     4321 cggatcgagg acatgatcgt gtggaccaag gtcggcgcgt tgaatcagcc gaccaacgtc
     4381 gaaaacgccc gcatcgaggg aacgacgctc gcctggcaag ggcggctggc cggtttcgac
     4441 ctgcaggcca gccacgactg gctcgacgcc gaggaccgca gcaaccaccg ccagttgccg
     4501 cgtcgggccc ggcacacggt ggcggcggcg gtcgcccgca gcgtcggcga ctgggactgg
     4561 cgcttcgggc tgacggcgag cggcaagcgc tacgagtaca aatcgaatgc gcgcgtcggc
     4621 ctgagcggct acactctcgc caacctttac gccgcctacc gtttttcgcc ggactgggcc
     4681 gtcttcgccc gcatcaacaa cctgttcgac cgcgactaca cgcttgccga cggttatgcg
     4741 acggcgggac gcaacgcctt catcggcatc cgctacagtc cgaagtaatc ccgcatgccg
     4801 acccgccgcc gcgccttcct gattcttgcc tcgctcgcct tgctcagcct ggccagtttc
     4861 gcgctggcgc tggcggccgg cagcctgcgc gtggcgccag cggaaatcgt cgccgccctg
     4921 ttcggccagg gcgacgggac cggtgccatg gtcgtccagt tgcgtctgcc gcgcgcgctc
     4981 gccggctttg cctgcggcgg cctgctggcg ctggccggcg cgttgatgca ggtgctgctg
     5041 cgcaatccgc tggccgatcc ctacgtgctg ggaatttccg gcggcgccgg ggttggcgcg
     5101 atgttcgcga tgctgcttgg gctgccgctg ctggctgtcg acggactcgc ctttgccggc
     5161 gccttcggcg ccatgttcct ggttttcggg ctggcgcacg gcgacggcag ctggacccag
     5221 acccgcctgc tgctcaccgg cgtcatcgtc gccgccggct gcggcgcgct gatcgcgctg
     5281 atgctgtcga tcgccgaaga gagcaagctg cacggcatgc tgttctggct gatgggcgat
     5341 ctcggccaga cctatacctg gtggccgacg ctgctggtgc tgatcctggc cctggcagtg
     5401 gcgatgcctt ttgcccgcga actcaacctg ctgtcgcgtg gcctgatgca ggcgcaggcg
     5461 ctgggggtgg cgatcgaccg cctgcgcgtc gccatttacc tgctcgcttc gctcgccacc
     5521 gccgcttcgg tgaccaccgc cggctcgatc ggcttcgtcg ggctggtcgt gccgcacctg
     5581 gtgcggctgg cgctcggcaa cgaccagcgc ctgctgctgc cggccgcggt gctggctggc
     5641 ggctcgttgc tggtctttgc cgacaccgcg gcgcgcaccg tgatcgcccc gcagcagttg
     5701 ccggtcggcg tcctcaccgc gctgatcggc gtgccggtct tcctcttcct gctgtcgagg
     5761 cagccgaagt gagcgccccc ttgcttgaag cccggcagct cggcgtcgat atcggcggca
     5821 agaccgttgt cgaccgtctg aacctgagcc tgcagccggg cgaaagcctg gccatccttg
     5881 gccgcaacgg tgccggcaag tcgacgctgc tgtccacgct ggccggcctg cgtccgccgc
     5941 gagccggcgc cgtgctgctc gatggcgaag attgcgccct gctgccgccg cgtcaggcgg
     6001 ccttgcgccg gggctggcta ggccagttcc agagcgaccc cttcggttcg accgtgctcg
     6061 aaaccgcgct caccggtcgc catcctcacc tcggccgctg ggactgggaa tcgaagcgcg
     6121 acgccgaact ggcgcgcggc gcgctcaagg cgctgggctt ggccggcatg gaggcgcgcc
     6181 aggtgcatac gctgtccggc ggcgaacgcc agcgcctctc catcgccacc ttgctgaccc
     6241 aggcgccacc gctctacctg ctcgacgaac cgctgtcgca tctcgacctc aaccaccaga
     6301 tggccgtcct cgaactgttt gccagcgccg cccgcgattg cggcgccgcc gtcgtcatgg
     6361 tcctgcacga cccggcgctg gcccaccgct tctgcgaccg ggcgctgctg gtctacggcg
     6421 acggccgttg cgcacagggt acggtcgata ccgtcctgac tgcggcaacg ctgtccggac
     6481 tctacggcta cgggctgcgg cagatcgaag accacggcca gcgctgtttc attccggagt
     6541 aagcccatga cgatcagcca cgaacaacgc atgcagaaga aaaaggccgt gatcgacggc
     6601 aagatcgacg ccgcccaaca ggaacgcggc gtgctggtga tcaacaccgg caacggcaag
     6661 ggcaagtcct cggccgcctt cggcgtcgtc gcccgggcgc tcggccatgg cttcaaggtc
     6721 ggcgtcgtcc agttcgtcaa gggccgctcg gacaccggcg aggaagcctt cttccgccag
     6781 cagcccaacg tcgcctggca tgtcggcggc gaaggcttca cctgggaaac ccaggacaag
     6841 gagcgcgatg ccagggccgc ccaggccgcc tgggacgttg cctgccagca cctggccgac
     6901 cccgacatcg gcctggtcgt cctcgacgaa atgacctacg ccttcaagta tggctggctc
     6961 gaccttgacg cggtgatcgc caggttgctc tcgcgtccgg ccctgcagca cgtcatcgtc
     7021 accggccgcg ccgcgccgca ggccttgcgc gacgccgccg acacggtgag cgagatcggc
     7081 atggaaaagc acgcctttca ggccggcgtg aaagccatgc ccggactgga atactgatgc
     7141 ccgcctgccc ggccctgctt gtcgccgcgc ccgcttccgg ccagggcaag accaccgtca
     7201 ccgccgcgct ggcccgcctg catgcgcggc aggggcggcg cgtcaccgtc ttcaagtgcg
     7261 ggccggattt cctcgatccg cagattcatg ccttcgccag cggtcgaccg tgccagaacc
     7321 tcgatctcgg catgtgcggc gaggacgacg cgcgttggcg actggcccgc gcggcggcgg
     7381 agtccgacct gatcctggtc gagggcgtca tggggctgtt cgacggcagg ccgtcggcgg
     7441 cctacatcgc cgagcgtttc gccattccgg tcatggcgct gatcgacgcc ggggccatgg
     7501 cccagacctt cggcgccgtc gcgcacggcc tggcgacata ccgcccggcg ctgccgtttg
     7561 gcggcgtgct cgccaatcgt gttgccagcc cgcgccacgc cgccatgctc caggaaagcc
     7621 tgaaacccgg catgggctgg ttcggtgccc tgccgaagag tggcgacagc ctgcccgagc
     7681 gccatcttgg cttgctccag gcggccgaga tcgacgacct cgcaagccgc ctcgacaagc
     7741 tggccgacgc gctcgccgcg agtgccacgg tcgacttgcc caaagcggta aatttcaccg
     7801 ccgccccgcc gccggccctg ccgcccttgc ttgccggccg gcgcatcgcc atcgcccgcg
     7861 acgcggccta cggcttcatc tacccggcga acctggaaac cctgttcggg ctcggcgccg
     7921 aattgcgctt cttctcgccg ctggccggcg atccgctgcc cgagtgcgac gccgtctggc
     7981 tgcccggcgg ctacccggaa ctgcacgcta ccgcgctgag cgccaatgtc ggcctgtggc
     8041 aggcgctgca cgcccacgtc gacgccggca agccgctgct cgccgagtgc ggcggcatga
     8101 tgagcctgtt cgaggaagtg gtggacaagg ccggccagac gtaccgcttc gccggtctgc
     8161 tgcccgggcg ggcggtcatg cagccgcgcc tggcggcgct cggcacgcag ttcgccgact
     8221 tgccggaagg ccggctggct ggccacacct tccattactc gaagagcgag acgccgctgt
     8281 cgccgctgtg tcgcgctgtc actgccgaag gccgcgaggg cgaggcgatc ttccgccggc
     8341 agcggctgac cgcctcctac gtgcatttct atttcccgtc gcagccgcag gcagcggcag
     8401 cgctgtttgg tgcttgaatt tccggttttt tcggatcgtc aattagcgtc accctgtttt
     8461 ttggaaggcg ttggctgcgt acaatgcccc cacttgttcc ccaaaggaga cattcatgaa
     8521 caaattcatc gccatggcgg cggccgtcat cctcggcctg acgctcaaca tcaacgacgc
     8581 cgaagccaaa cgcctgggtg gtggtagctc gttcggcatg cagcgccagg ccacgccgcc
     8641 gagccgtaac gtccagcaag cgccgcatca ggcacccgct gcggcgccgc aggcgcagcc
     8701 gaagcgctca tggatggggc cgattgccgg tctcgccgcc ggtctcggtc tggccgcgct
     8761 ggcttcgcac ttcggtttcg gcgaaggact ggccaacttc atgatgatcg ccctgctggc
     8821 tttcgccgtg atcgccgcca tcggcttctt catgcgcaag aaggccggcg ctcaggcgca
     8881 gggcatgcag tacgccggcg ctggtgccaa ctacggcggc cagccgggca atgtggcgcg
     8941 gccggatttc acgccggcag gtggttcgtc cttcacgtcg gcggcccagg ccgttgaatc
     9001 gactggcggc aacattccgg ccgacttcga cgtcgaagcc ttcgtgcgca acgccaaggt
     9061 caatttcatc cgcctgcagg cggccaacga tgccggcaac ctcgacgaca tccgtgaatt
     9121 caccgcgccg gaaatgttcg ccgaaatcaa gatggcgatc gaggaacgca agggtgccag
     9181 ccaggagact gatgtcgttc atctcgatgc ccaggtcatc gacgtcgtcg aggaagccag
     9241 ccgttacatt gtcagcgtgc acttcagcgg ccagatccgc gaggagcgta acggcccggt
     9301 cgagaatttc tcggaaatct ggcacatgac caaacccctc agcggcaacc gcggctgggt
     9361 catcgccggc atccagcaag ttcagtaagc gctgccgcgc caggaggcaa aagccgaccg
     9421 gggccacccg gtcggttttt tttcgctcag ggattctccg ccagggcgac gacatcgccg
     9481 atgacgagca gggccggcgg cgcgaccgcg tggcgccggg cgacttccgg tagcgtcgcc
     9541 agtgtcgccg gatagatctt ctggttcggc caggtggcgc ggtagatcag cgctgccggc
     9601 gtcgttccgg gcagcccgtg cgcctgcaac tgccgggcga tgatgtcgag ccccttgacg
     9661 cccatgtaga tcgcgatcgt ctgctgcggc tgggcgagcg cctgccagtt caggtcgatg
     9721 ctgtgatcct tgtagtgtcc ggtgacgaag acgcagctct gggcatggtc gcggtgcgtc
     9781 agcggaaagc cgaaggcggc agcggcgccg agtgcggcgg tgatgccggg gacgatctcg
     9841 acttcgatgc cgtgctcgac gagcagcgcc atctcctcgc cgccgcgccc gaaaacgaag
     9901 ggatcgccgc ctttcagccg gaccacgcgg cggctggcgc gggctaggtc gagcaggcgc
     9961 cggctgattt cctcctgcgg cagcgtgtgg ttgccggccg atttgccgac gtagatgcgc
    10021 tccacggtcg acgggcagaa atcgacgatt tccgggctga ccaggttgtc gtagagcatc
    10081 acctcggcct gctgcaggcg ccggtaggcc ttcagcgtca gcagctcggg gtcgccgggg
    10141 ccggcgccga tcagcgcgac gcgcccggcc gggaattcgc taccgcggct caacatgctt
    10201 cgtctccgcc gccgttgcgg tgaaagacca ggtcttcgac gaccgcgccg cccttcaggt
    10261 gctgctcgac gatgcggtcg atgttcgcgt cgctgatgtt gtaataccag acgccgtccg
    10321 gctgcacggc gacaatcggc ccgccctggc aggcagcgac gcagccggtg cgcgtgcgct
    10381 tgacccgcgc tgggccggca tcgacgccga ggccggcggc gcgcagcttg ccttggagcg
    10441 cgttgaacag cgcttcgccc tcgccgttct gcgtacagcg cgggccggtg cacaccagga
    10501 tctgccggct gtagtcgcgg atcttcggct tgatgatctc gctctcgtcg tgctcagcct
    10561 tcaatttcgg cctcctcgtt ttccggcggc agttcgctct gcaatgcggc ggcgatgtag
    10621 tcctccggca ggccatagtc ggcggccagt tcggcggcgc tgcggccgct cgcctggatc
    10681 gtcgccagcc agccgttgag gccggtggac aaggaacgcc cggcttgttc gccggcatgc
    10741 gtgttgcgct cgccggcctc caccgcgtac ttgttggcgt agccgcgcgg cgtcaccatc
    10801 aggccgtgct tgatgaaggt gttggaattg ccgatcagta ccgtcgacag catgccgatc
    10861 tcggcctcgg ccatgcgctc cagcgtcgtc agctcgatgc gctgcttcgg gcggtggccg
    10921 gacttgacga tggccaccgg cgtctgcggg tcgcgatggc gcaggaacag gcgctgcgct
    10981 tcgacgatct gctgcgtgcg ccgaccgctc ttcgggttgt acagcgcgac gacgaaatcg
    11041 gcataggcca ccgcgtcgag ccggcgggcg atggtcggcc agggcgtcag caggtcggac
    11101 agcgaaatgg cgcagaaatc gtgggtcagc ggcgcaccga ccagcgccgc gcaggtgttg
    11161 atcgccgagg cgccggggac gatctcgacc tcgatatcgg cgtccggcgt ccacccggcc
    11221 tggaacagca cctcgaaggt cggcccggcc atgccgtaga cgccggcatc gcccgaggaa
//
LOCUS       DAOSDX010000003         9698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2635_length_9698_cov_3.583843, whole genome shotgun sequence.
ACCESSION   DAOSDX010000003 DAOSDX010000000
VERSION     DAOSDX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2635_length_9698_cov_3.583843"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /gene="prmA"
                     /locus_tag="PLN02_00140"
     CDS             complement(<1..759)
                     /gene="prmA"
                     /locus_tag="PLN02_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOI50760.1"
                     /translation="MAWLSVSFLTDAVSAEPFCDALLENGALSASIEDADAGTADEKP
                     QFGEPGSVTTPGWDRSRIVALFETDAAVDAMLAAAAAAAGLSAPTEYTAETIEEQNWV
                     QLTQSQFDPIRVSDRLWIVPSWHESPDPAAINLILDPGMAFGTGSHPTTRLCLEWLER
                     TVRPGDSVLDYGCGSGILAIAAGKLGAADVAGVDIDPQAVAAAVANAERNAVAARFSD
                     SGQALAGEYDVVVANILSNPLRVLAPAICAHVRRG"
     gene            complement(760..2118)
                     /gene="accC"
                     /locus_tag="PLN02_00145"
     CDS             complement(760..2118)
                     /gene="accC"
                     /locus_tag="PLN02_00145"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOI50761.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKL
                     ADESVCIGPAASAQSYLNVPAIISAAEVTDAQAIHPGYGFLSENADFAERVETSGFVF
                     IGPRAETIRLMGDKVSAKDAMKAAGVPCVPGSEGALPDDPKEIVRIARAIGYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVGMTRQEAGTFFGNPAVYMEKYLENPRHVEIQVLADQ
                     YRSAIYLGERDCSMQRRHQKVIEEAPAPLIPPRLISRIGERCAEACRKIGYRGAGTFE
                     FLFENGEFYFIEMNTRVQVEHPVTEMITGIDIVQEQIRIAAGEKLRYRQKDVNFRGHA
                     IECRINAEDPFTFVPSPGKIEFYHPPGGPGIRVDSHIYQGYRVPSHYDSMVAKVIAYG
                     DTREQAIRRMRVALSEMSIQGIKTNIALHQELMLDARFIEGGSSIHYLEQKLAAMNEV
                     KQ"
     gene            complement(2127..2585)
                     /gene="accB"
                     /locus_tag="PLN02_00150"
     CDS             complement(2127..2585)
                     /gene="accB"
                     /locus_tag="PLN02_00150"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOI50762.1"
                     /translation="MDLRKLKKLIDLVQESGISELEVTEGEEKVRIAKHFGAVAAPVQ
                     HYAMPAPMPMPAAGAPTTSSVNLDDEDDLPEGHVMKSPMVGTFYRSPSPGAAAFVQVG
                     SVVKKGDTLCIIEAMKLLNEIEAEVDGTVKAILLDNGEPVEFGEPLFVIG"
     gene            complement(2645..3127)
                     /gene="aroQ"
                     /locus_tag="PLN02_00155"
     CDS             complement(2645..3127)
                     /gene="aroQ"
                     /locus_tag="PLN02_00155"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOI50763.1"
                     /translation="MTKRKTASKPAEKARILVVHGPNLNLLGTREPEHYGRMTLADIN
                     LALARLAESVGVELEAFQSNHEGALIERIHAAREQGVRAIIINPAAYTHTSVALRDAL
                     AAVAIPFVEVHLSNVHARESFRHHSYFSDLAIGVICGLGHEGYRFALEFLLNRINLDQ
                     "
     gene            complement(3167..3757)
                     /locus_tag="PLN02_00160"
     CDS             complement(3167..3757)
                     /locus_tag="PLN02_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50764.1"
                     /translation="MPRHERLRNGSSVRSTIAAAAARLMAEDGISDYHHAKKKAARQL
                     GLPDNVGFPDNAEVEAELRAYRELYQGDIHPEMLAAMRHSALDLMELLAAFNPWLTGS
                     VLDGTAGEHSSIDILLFADSAKEVEIFLLNRGIDVNHAQPRHEKVEAVLQVETDTVDA
                     NLIILPPHLERVSLKHRDGRPRERMRAAELHLLLKE"
     gene            3808..5154
                     /gene="mpl"
                     /locus_tag="PLN02_00165"
     CDS             3808..5154
                     /gene="mpl"
                     /locus_tag="PLN02_00165"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HOI50765.1"
                     /translation="MHIHILGICGTFMGGIAQLAVASGHRVTGCDANVYPPMSDQLRQ
                     AGIELIEGFASEQLALAPDVFVVGNAISRGNPLLEAILDRGLPYVSGPQWLAENVLQG
                     RWVLGVAGTHGKTTTSAMLAWILEDAGLAPGFLIGGVPLDFGVSARLGETPFFVIEAD
                     EYDTAFCDKRSKFIHYRPRTAILNNLEFDHADIFADLAAIETQFHHLVRTMPASGLIV
                     ANAGEASLERVLQRGCWTPVERFNAPGGYRATGDDADGSLILHDASGEIGRCQWQLTG
                     EHNRANACAALLAARHVGVPFAKGLEALARFANVKRRMEVRGVVNGVTVYDDFAHHPT
                     ALATTLAGLRRKVGTARILAIFEPRSNTMKLGTMKALLPDSLRDADLAFCYAGNLGWD
                     AAAALAPLGERAVVEEDLAALVCTITAVARPGDHLLVMSNGGFGGIHQKLLEGLAR"
     gene            5151..5738
                     /locus_tag="PLN02_00170"
     CDS             5151..5738
                     /locus_tag="PLN02_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOI50766.1"
                     /translation="MKRRDFITLAGALVLTACGGEAPGFRNTDLSSGQLFPRATLYDG
                     QGRARSFEEFRGKAVIVFFGYTSCPDICPSALRKYASLLRTLRDRDAARVQVIFISVD
                     PERDTPERTDTFAKWFHPDFLGLSGTEEQIAEVTRQFKVVYGKKEIGGGMAYVIDHTA
                     AAYVLDPTGQLRLMLAETAPLEPIIADLQSLLTAP"
     gene            complement(5796..8072)
                     /locus_tag="PLN02_00175"
     CDS             complement(5796..8072)
                     /locus_tag="PLN02_00175"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOI50767.1"
                     /translation="MEKDLREAALEYHRWPTPGKISVRPTKGLTNQRDLALAYSPGVA
                     AACDAIVEDEATAALYTSRANLVGVVTNGTAVLGLGNIGPLASKPVMEGKGCLFKKFA
                     GIDVFDIELAENDPDKLIDMIAALEPTLGGVNLEDIKAPECFYIEQKLKERMNIPVFH
                     DDQHGTAIISGAAMLNGLKVVGKKIDEIKVVVSGAGAAAISCVNLWCDLGVKRENITI
                     CDSKGVIYVGRPGGMDETKARYAQNTDKRTLADAMVGADIFLGLSAAGVVNQDMVKSM
                     AEKPMVFALANPTPEIMPELVKEVRPEAIIATGRSDYVNQVNNVLCFPFIFRGALDVG
                     ATRITEEMKMASVRAIAELAEAEVTDEVAMAYPGAVLSFGPEYLIPKPFDPRLIVKIA
                     PAVAQAAMDSGVATRPITDWAAYRAKLSEFVYHTGVGMRAIFQAARQAKGKRIIFAEG
                     EEERVLRAVQVVIEEKFARPILIGRPDIIELRLDKAKLRIKPGVDFDIVNPQSDERYR
                     ECWTAYHKLMARRGVTPAIAKEAMRRKPTVIGAMLLKLGYADGMICGMTGQYSHHLGV
                     INQIIGKRSGVNTLAAMNYLMLPGRSLFICDTHVNENPNAEEIAEMTLMAAEQVKRFG
                     SQPKVALLSHSNFGSGTSESARKMGVAAGLISAMSSGELEVDGEMHGDSALSEEIRRE
                     ANPDSPLKGEANLLVMPNIDAANISYNLLKMTGGEGVTIGPILLGAARPVHVLTSTAT
                     VRRLVNMTALAVVESMER"
     gene            complement(8202..8996)
                     /locus_tag="PLN02_00180"
     CDS             complement(8202..8996)
                     /locus_tag="PLN02_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI50768.1"
                     /translation="MPDALLEFSGVAYAWPDGRPVFADLSFRIAASEKVVLLGANGAG
                     KSTLLRLANGLLPPSAGSIAWQGHALSPSLLRQRDFARSFRQANVLLFQHPEAMLFNP
                     TVREEIAYGPQQLGLADSAARVERWAAELGLQTLLDAAPFTLSGGQKQKVALACLLAL
                     DPQFLALDEPSASLDPATVGWLIDTLLDSGRTLLISTHNLGLAAELGERALILDGNGR
                     LLYDGPLEHALGDLPLLQAAGLAHRHRHRHGGETHAHLHVHDWESS"
     gene            complement(8989..9543)
                     /locus_tag="PLN02_00185"
     CDS             complement(8989..9543)
                     /locus_tag="PLN02_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50769.1"
                     /translation="MNLARVWLLAYVAAVLAISFIHQPVVLAAGLAGALAAAGAARWR
                     LLQRAALALLLVNASVSLAYAGLAWWQGRPLGDALLLINLRVLLLVFLGFWLVDRVRL
                     LDALAGWPVLSMIATLALGQIAVYRRLLDDFRLAFRSRNPAPPSAAAMLRHAGAQAGC
                     LLDKSLHDAEQAAQAMRSRGAFDA"
BASE COUNT         1576 a         2971 c         3382 g         1769 t
ORIGIN      
        1 gccgcgccgg acatgggcgc agatggcggg agcgagaacg cgcagcggat tggacaggat
       61 gttggccacg acgacatcat actcaccggc gagcgcctgt ccagaatcgg agaagcgcgc
      121 ggccacggcg ttgcgttcgg cgttggcgac ggcggcggcg accgcctgcg ggtcgatgtc
      181 gaccccggca acgtcggcgg caccgagttt gccggcggcg atggcgagga tgcccgagcc
      241 gcagccatag tcgagcacgc tgtcgccggg gcggacggtg cgttccagcc attccaggca
      301 gagccgcgtc gtcgggtgcg agccggtacc gaaggccatg cccgggtcga ggatcaggtt
      361 gatcgccgcc ggatcgggtg attcgtgcca ggaggggacg atccacaggc gatcggagac
      421 gcggatcgga tcgaactgcg actgggtcag ttgcacccag ttctgttcct cgatggtttc
      481 ggccgtgtat tcggtcggcg cgctcaggcc ggcggcggcc gccgccgcgg caagcatggc
      541 gtcaacggca gcatcggtct cgaacagggc gacgatgcgc gagcgatccc agcccggcgt
      601 ggtcaccgaa ccgggttcgc cgaactgcgg cttttcgtcc gcggtgccgg cgtcggcatc
      661 ctcgatcgac gccgacagcg cgccgttttc gagcaacgcg tcgcagaaag gctcggcgct
      721 gaccgcatcg gtcaggaagc tgacgctcag ccaggccatt tactgcttga cctcgttcat
      781 cgccgccagc ttctgctcca gatagtggat gctggagccg ccttcgatga agcgggcgtc
      841 gagcatcagc tcctggtgca gcgctatgtt ggtcttgatg ccctggatgc tcatctccga
      901 cagcgcaacg cgcatgcggc ggatcgcctg ttcgcgggtg tcgccgtagg cgatcacctt
      961 ggcgaccatc gaatcgtagt gcgacgggac gcggtagccc tggtagatgt gcgaatcgac
     1021 gcggatgccg gggccgccgg gcgggtgata gaactcgatc ttgccgggag atggcacgaa
     1081 cgtgaaggga tcttcggcgt tgatgcggca ttcgatggcg tggccgcgga aattcacgtc
     1141 cttctgccgg tagcgcagct tctcgccggc ggcgatccgg atctgttcct ggacgatgtc
     1201 gatgccggtg atcatctcgg tcaccgggtg ttcgacctgg acgcgggtgt tcatttcgat
     1261 gaaatagaac tcgccgttct cgaacaggaa ttcgaaggta ccggcgccgc ggtagccgat
     1321 cttgcggcag gcttcggcgc agcgttcgcc gatgcgcgag atcaggcggg gcgggatcag
     1381 cggcgctggc gcttcctcga tcaccttctg gtggcggcgc tgcatcgagc agtcgcgttc
     1441 gccgaggtag atggcgctgc ggtactggtc ggcaagcacc tggatttcca cgtggcgcgg
     1501 gttttccagg tacttctcca tgtaaacggc aggattgccg aagaaagtgc cggcttcctg
     1561 gcgggtcatg ccgaccgcgt tgaccagtgc cgcttcggta tggacgacac gcatgccgcg
     1621 accgccgccg ccgccggccg ccttgatgat caccgggtag ccgatggcac gggcgatgcg
     1681 gacgatttcc ttggggtcgt cgggcaacgc gccttcggag ccggggacgc agggcacgcc
     1741 agcggccttc atcgcgtcct tggccgagac cttgtcaccc atcaggcgga tcgtttcggc
     1801 gcgcgggccg atgaagacga aaccggaggt ttcgacgcgc tcggcgaagt cggcgttctc
     1861 ggagaggaaa ccgtaaccgg ggtggatcgc ctgggcgtcg gtgacttcgg cggcggaaat
     1921 gatcgccggc acgttcaggt agctttgcgc cgaggcagcc ggaccgatgc agaccgattc
     1981 gtcggcgagc ttgacgtact tggcctcgcg gtcggcttcc gaatggacga cgacggtctt
     2041 gatgcccatc tcgcgacagg cgcgctggat acgcagggcg atttcgcccc ggttagcgat
     2101 gaggattttc tcgaacatcg gcatgatcag ccaatcacga acaggggctc gccgaattcg
     2161 accggttcgc cgttgtcgag caggatggcc ttgaccgtgc cgtcgacctc ggcttcgatc
     2221 tcgttgagca gcttcatcgc ttcgatgatg cacagcgtgt cgcccttctt gacgacactg
     2281 ccgacctgga cgaaggcggc ggcgccgggc gacggcgagc ggtagaaagt gccgaccatc
     2341 ggcgacttca tgacgtggcc ttccggcagg tcgtcctcgt catcgagatt gaccgagctg
     2401 gtcgtcggcg cgccggccgc gggcatcggc atcggggccg gcatggcgta gtgctggacg
     2461 ggggcggcga cggcgccgaa atgcttggcg atgcggacct tttcctcgcc ctcggtgact
     2521 tcgagttccg aaatgccgga ctcctgaacg aggtcgatga gttttttcag cttgcgcaga
     2581 tccatgatga ctccctaaat tttttgcgca cgatgggcgc atgatcacga cagttgtcgc
     2641 tagattactg atccagattg attctgttga ggaggaattc gagggcgaag cggtagccct
     2701 cgtgacccag tccgcagatg acgccgatgg ccaggtcgga gaaatacgaa tggtgccgga
     2761 agctttcccg ggcatgcacg ttggacaggt gcacctcgac gaagggaatg gcaacggcgg
     2821 ccagtgcgtc gcgcaaggcg acgctggtgt gcgtgtaggc ggcggggttg atgatgatgg
     2881 cgcgcacgcc ctgctcgcgg gcggcgtgga tgcgttcgat cagcgcacct tcgtggttgc
     2941 tctggaacgc ttcgagttcg accccgacac tttccgccag acgcgccagc gccaggttga
     3001 tgtcggccag cgtcatgcgg ccgtagtgtt cgggttcgcg cgtcccaagc aggttcagat
     3061 tcgggccatg aacgaccagg atacgggcct tttccgccgg cttggaagca gtttttcgct
     3121 tcgtcatggc ggcaagtttg ccgcaaatcg ccgcaacttg tccagtctat tccttaagta
     3181 agagatgtag ttcggcggcc cgcatgcgct cgcgcggcct gccgtcgcgg tgtttcaggc
     3241 tgacccgctc gaggtgcggc ggcaggatga tcaggttggc gtccacggtg tcggtttcca
     3301 cctgtagcac ggcttcgacc ttctcgtgac ggggctgggc gtggttgaca tcgatgccgc
     3361 ggttgagcag gaaaatctcc acttccttgg cgctgtcggc gaacagcagg atgtcgatcg
     3421 atgagtgctc gccggccgtg ccgtcgagca ccgagccggt cagccaggga ttgaaggcgg
     3481 ccagcaattc catcagatcg agtgccgaat ggcgcatcgc cgcgagcatt tccgggtgga
     3541 tgtcgccctg gtaaagctcg cggtaggcgc gcagttcggc ctcgacctcg gcgttgtcgg
     3601 gaaagcctac attgtccggc agaccgagct ggcgggccgc cttcttcttg gcgtggtggt
     3661 agtcgctgat gccgtcttcg gccatcagcc gggcggcggc ggcggcgatg gtggaccgga
     3721 cgctggaacc gtttcgcagc ctttcgtggc ggggcatggt gtctcgctcg gggtagaatc
     3781 gggtccattc taaccacagt cagcgccatg cacattcaca ttctcggcat ctgcggcacc
     3841 ttcatgggcg gtatcgccca gcttgccgtt gcctccgggc accgtgtcac cggatgcgat
     3901 gccaacgtct atccgccgat gagcgaccag ctgcgtcagg ccggcatcga actgatcgaa
     3961 ggttttgcca gcgagcaatt ggcgctggcg cccgatgtct tcgttgtcgg caacgcgatt
     4021 tcgcgcggca acccgttgct cgaagccatc ctcgaccgcg gtctgcccta cgtttccggg
     4081 ccgcaatggt tggccgaaaa cgtcctgcag ggacgctggg tgctcggcgt cgccggtacc
     4141 catggcaaga cgacgaccag cgcgatgctg gcctggatac ttgaggacgc cggtctcgcg
     4201 ccgggtttcc tgatcggtgg cgtgccactc gacttcggcg tctcggcgcg gcttggcgaa
     4261 acgcccttct tcgtcatcga ggccgacgaa tacgacaccg ccttctgcga taagcgttcc
     4321 aagttcattc actaccggcc gcggacggcg atcctcaaca acctggaatt cgatcatgcg
     4381 gatatcttcg ccgatctggc ggcgatcgaa acgcagtttc accatctggt gcggaccatg
     4441 ccggcttccg ggctgatcgt cgccaacgcc ggtgaagcca gtctcgaacg cgtcctgcaa
     4501 cgcggatgct ggacgccggt cgagcgtttc aacgcgcccg gcggctatcg cgcgaccggc
     4561 gacgacgccg acggcagcct gatcctgcat gacgcgagcg gcgagatcgg ccgctgccag
     4621 tggcagctga ctggcgagca caaccgcgcc aatgcctgcg ccgcgctgct ggcggcgcgc
     4681 catgtcggcg tccccttcgc caaggggctg gaggcgctgg cgcgcttcgc caacgtcaag
     4741 cggcgcatgg aagtgcgcgg cgtggtcaat ggcgtcaccg tttacgatga tttcgcgcat
     4801 cacccgacgg cgttggcgac gacgctcgcc ggtttgcggc gcaaggttgg cacggcgcgc
     4861 atcctggcca tcttcgagcc aaggtccaac accatgaagc tggggacgat gaaggcgctg
     4921 ctgccggata gcctgcgcga tgccgacctc gccttctgct acgccggcaa cctcggctgg
     4981 gatgcggcgg cggcgctggc gccgctgggc gaacgtgccg tcgtcgagga agacctcgcg
     5041 gcactggttt gcaccatcac cgcggtcgcg cggccgggcg atcatctgct ggtgatgagc
     5101 aacggcggct tcggcggcat tcatcaaaaa ctgctggagg gactggcccg atgaagcgcc
     5161 gcgatttcat taccctggcc ggtgccttgg tgctgaccgc ctgcggcggt gaggcgccgg
     5221 gcttccggaa taccgatctg tcgtccggtc agctgtttcc ccgggcgacg ctctacgacg
     5281 gtcagggccg cgcccgcagt ttcgaggagt tccgcggcaa ggcagtgatc gttttcttcg
     5341 gctacaccag ttgcccggac atctgcccga gcgcgctgcg caagtacgcc agcctgctgc
     5401 gtacgctgcg cgaccgcgac gcggcgcggg ttcaggtcat cttcatttcg gtcgatccgg
     5461 agcgcgatac gccggagcgc accgatacct ttgccaagtg gttccatcca gattttctcg
     5521 ggctttccgg aaccgaggag cagattgccg aagtgaccag gcagttcaag gtggtttatg
     5581 gcaagaagga gatcggtggc ggcatggctt acgtcatcga ccatactgcc gccgcctacg
     5641 tcctcgaccc gaccggtcag ttgcgcctga tgctggcgga gacggcgccg ctggagccga
     5701 tcatcgccga tctgcagagc ttgctgacgg cgccatgaaa aaggccccgc cagttggcgg
     5761 ggcctcgctg cgcggcgatc cggcaggtgc cgggatcagc gttccatcga ttcgacgacg
     5821 gccagcgcgg tcatgttgac caggcgacgg acggttgccg tcgaggtcag cacatgcacc
     5881 ggacgggccg caccgagcag aatggggccg atggtgacgc cttcgccgcc ggtcatcttc
     5941 aacaggttat acgagatgtt ggcggcgtcg atgttgggca tcaccagcag gttggcttcg
     6001 cccttgagcg gcgaatccgg attcgcctcg cggcggattt cctcgctcag cgccgagtcg
     6061 ccgtgcattt cgccatcgac ttccagctcg ccggacgaca tcgccgagat caggccggcg
     6121 gcgacgccca tcttgcgcgc cgattccgaa gtgccggaac cgaagttgga atgcgagagc
     6181 agcgcgacct tcggctggct gccgaagcgc ttgacctgct ccgccgccat cagcgtcatt
     6241 tcggcgatct cttcggcgtt cgggttctcg ttgacgtggg tgtcgcagat gaacagcgag
     6301 cggcccggca gcatcaggta gttcatcgcg gccagggtgt tcacgccgct gcgcttgccg
     6361 atgatctggt tgatcacgcc gaggtgatgg ctgtactggc cggtcatgcc gcagatcatg
     6421 ccgtcggcgt agccgagctt gagcagcatg gcgccgatca ccgtcggctt gcggcgcatc
     6481 gcttccttgg cgatcgccgg cgttacgcca cgacgggcca tcagcttgtg ataggcggtc
     6541 cagcactcgc gatagcgttc gtcggactgc ggattgacga tgtcgaaatc gacgcccggc
     6601 ttgatccgca gcttggcctt gtcgagccgc agttcgatga tgtcgggacg gccgatgagg
     6661 atcggacggg cgaatttttc ttcgatcacc acctgaacgg cgcgcaacac gcgctcttct
     6721 tcaccttccg cgaagatgat gcgcttgccc ttggcttgcc gtgcggcctg gaagatggcg
     6781 cgcatgccga cgccggtgtg atagacgaat tccgacagct tggcgcggta ggcggcccag
     6841 tcggtgatcg gccgcgtggc gacgccggaa tccattgctg cctgggcgac ggccggggcg
     6901 atcttgacga tcaggcgcgg gtcgaagggc ttcgggatca ggtattccgg gccgaaggag
     6961 agcacggcgc ccggataggc catcgccact tcgtcggtga cttcggcttc ggccagttcg
     7021 gcgatggcgc gcacggaagc catcttcatt tcttcggtga tgcgggtggc gccgacgtcg
     7081 agcgcaccgc ggaagatgaa ggggaagcag aggacgttgt tgacctggtt cacgtagtcg
     7141 gaacggccgg tggcgatgat cgcctcgggg cggacttcct tgaccagttc cggcatgatt
     7201 tccggggtcg ggttggccag tgcgaagacc atcggcttct cagccatgct cttgaccatg
     7261 tcctggttga ccacgccggc cgccgacaga ccgaggaaga tgtcggcgcc gaccatcgca
     7321 tcggcgagcg tgcgcttgtc ggtgttctgg gcgtagcggg ccttggtttc atccatgcca
     7381 ccggggcggc cgacgtagat gacgcccttg gagtcgcaga tggtgatgtt ctcgcgcttg
     7441 acgccgaggt cgcaccacag gttcacgcag gagatggcgg cggcgccggc gccggacacc
     7501 accaccttga tttcgtcgat cttcttgccg acgaccttga ggccgttgag catggcggcg
     7561 ccggagatga tcgcggtgcc gtgctggtcg tcgtggaaga ccggaatgtt catcctttcc
     7621 ttcagcttct gctcgatgta gaagcattcc ggcgccttga tgtcttccag gttcacaccg
     7681 ccgagcgtcg gctcgagcgc ggcgatcatg tcgatcagct tgtccgggtc gttctcggcc
     7741 agttcgatgt cgaagacgtc gatgccggcg aacttcttga acaggcagcc cttgccttcc
     7801 atcaccggct tcgaggccag aggcccgatg ttgccgaggc cgaggacggc ggtgccgttg
     7861 gtgacgacgc cgaccaggtt ggcgcgcgag gtgtagagcg cagcggtcgc ttcgtcctcg
     7921 acgatcgcgt cacaggcagc ggcaacgccg ggcgagtagg ccagcgccag gtcgcgctgg
     7981 ttggtcaggc ccttggtcgg acggacggaa atcttgcccg gggtgggcca gcggtgatat
     8041 tcgagtgcgg cttcgcgcaa atccttttcc acggataatc ctcggcaaac aagttgggga
     8101 aaaccctgta ggttactcca aagcttcgca acagggtgtg taattatgga ttatggggga
     8161 gccggccgct tttgactacc ggcggggaaa tggcgtccgg atcacgacga ttcccagtcg
     8221 tgcacgtgca gatgggcgtg cgtttcgccg ccgtggcgat gccggtggcg gtgcgccagg
     8281 ccggctgcct gcagcagcgg caggtcgccc agcgcatgtt cgagcgggcc gtcgtagagc
     8341 aggcggccgt tgccgtcgag aatcagcgcg cgttcgccga gttcggcggc gaggccgagg
     8401 ttgtgcgtgc tgatcagcaa ggtccgtccg gaatcgagca gggtgtcgat cagccagccg
     8461 accgtcgccg ggtcgagcga ggcgctcggt tcgtcgaggg cgaggaattg cgggtcgagg
     8521 gcgagcaggc aggccagcgc gaccttttgt ttctggccgc cggagagcgt gaacggtgcc
     8581 gcatcgagca gcgtttgcag gccgagttcg gccgcccagc gctcgacgcg ggcggcgctg
     8641 tcggcaaggc cgagctgctg cgggccatag gcgatttcct cgcgcaccgt cgggttgaac
     8701 agcatcgctt ccgggtgctg gaagagcagc acgttggcct gccggaagga gcgtgcgaaa
     8761 tcgcgctgac gcagcagcga cgggctgagt gcgtggccct gccaggcgat gctgccagcg
     8821 ctcgggggca gcaggccgtt ggcgaggcgc agcagcgtcg atttgccggc gccgttggcg
     8881 ccgagcaaca ccaccttctc gctggcagcg atgcgaaagc tcaagtcggc gaaaaccggc
     8941 cggccatccg gccaggcgta ggcgacaccg gaaaattcaa gcagcgcgtc aggcatcgaa
     9001 agctccccgg gaacgcatcg cctgtgccgc ctgttcggcg tcgtgcaggg atttgtcgag
     9061 caagcagccg gcttgggcgc cggcgtgccg gagcatggcg gcggcgcttg gcggcgccgg
     9121 gttgcggctg cggaaggcga gccggaagtc gtcgagcagc cggcgataga cggcgatctg
     9181 gccgagcgcc agggtagcga tcatcgacag cacgggccag ccggccagcg cgtcgagcag
     9241 gcgcacgcga tcgaccagcc agaagccgag aaagaccagc agcagcacgc gcaggttgat
     9301 cagcagcaag gcgtcgccga gcggccggcc ctgccaccag gcgaggccgg cgtaggccag
     9361 gctgacgctg gcgttgacca gcagcagcgc cagcgccgcc cgctgcagca gccgccagcg
     9421 cgccgcgccg gcggcggcca aggcgccggc caggccggcg gcgagcacca ccggttggtg
     9481 gatgaagctg atcgccagca cggcggcgac ataggcgagc agccagacgc gcgccaggtt
     9541 catgcggcga cccagccctt gcgcttggcg tagcgccaga cgaggacggg cagcgccgct
     9601 tcgccgatgc cgatcagcag gtgcggcagc agcacggccg gcacggtgac ggcgaaaccg
     9661 agcgggaaga acagcgggct gccgtcctcg cgctgggc
//
LOCUS       DAOSDX010000004         9650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2653_length_9650_cov_3.690047, whole genome shotgun sequence.
ACCESSION   DAOSDX010000004 DAOSDX010000000
VERSION     DAOSDX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_9650_cov_3.690047"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="PLN02_00190"
     CDS             complement(<1..1841)
                     /locus_tag="PLN02_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOI50770.1"
                     /translation="MTQEAQQETRSTCPYCGTGCGVVISHSAGRIIGVRGDPEHPANF
                     GKLCTKGANLHRTVQLDGRLVQPLWREQRAAPLQALGWDEALDQAARRFAETIREHGP
                     DSVAFYISGQLLTEDYYVFNKLARALVGTNNIDSNSRLCMSSAVAGYKATLGMDSVPC
                     CYEDIDLADLLFISGANTAFAHPILFRRIEAAREARPDLKIVVVDPRRTDTAAMANLH
                     LQIAPGSDLYLFAAMLQVLAAEGLVDAAYIADHTEGYAALLEQLGSVTPEVAAGVCQV
                     PAADIVQAARWFGQAAAPLSFWCQGLNQSGHGTANSAALIHLHLATGKIGRPGMGPFS
                     LTGQPNAMGGREVGAMANLLSAHRDLKNPADRAEMADFWGIPAIPAQPGLMAVQLFEA
                     VRRGEIKALWIACTNPAHSLPDQQRVREALAACPFVVVQDAFADTDTCAHADLLLPTA
                     TWGEKDGTVTNSERRITRVRAAVAAPGEARADWRIARDFALALGRQLDRPDAARLFDF
                     PSPAAVFAEHARSTRGRDLDIAGLDYARLEQGAVQWPYAAGQANGKARLYSDGVFPTA
                     SGRARFAPIELRLNGEAPDAAYPLRLNTGRLRDQWHGMSRTGRSGRL"
     gene            complement(1910..3109)
                     /locus_tag="PLN02_00195"
                     /pseudo
     CDS             complement(1910..3109)
                     /locus_tag="PLN02_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(3120..3872)
                     /gene="cobA"
                     /locus_tag="PLN02_00200"
     CDS             complement(3120..3872)
                     /gene="cobA"
                     /locus_tag="PLN02_00200"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOI50771.1"
                     /translation="MATVYLVGAGPGAADLLTLRAARILAQEAEVVLADDLVSAEVLA
                     LVRPQARILHVGKRGGRASTPQEFIERLMLRYARQGHCVVRLKGGDPFLFGRGGEEML
                     RLRGAGHRVEVVSGLTAGIAVPATLGIPVTHRGLAAGVTFVTARAGTQGSPAPDWAAL
                     AKTGTTLVVYMGLALLPEISAALIGGGLAPSTPVAVIARGCQPTQREVLARLDDVAAA
                     VEAAALPAPALIVVGPVVDLAACCDMALRLAA"
     gene            complement(4239..5978)
                     /locus_tag="PLN02_00205"
     CDS             complement(4239..5978)
                     /locus_tag="PLN02_00205"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HOI50772.1"
                     /translation="MPSPESGPRVSLGQHSLAGEHPVNQDFHGALLPGGHLLAAKGIA
                     LAIADGISSSRVGHIASQTAVRSFLDDYYATAEAWTVRRSAQRVLDAVNAWLYSQTMH
                     GAGRFDRDSGYVCTFSALILKARQLHLLHVGDARVYRLHAQALEQLSEDHRVRHDAGS
                     YLARALGVGSHVEIDYRCWDAEPGEIYLLATDGAHEFLDAAAVHAALDQHGDALDAVA
                     ATLVEAARAAGSRDDATVQLLRIDALPPPAADQLRAQRQTLPLPPPLRPGMSFEGFSI
                     VRELQVSARSHVYLAEDEASRTPVVLKLPSTDLRENAAYLDGFVLEEWVARRVDSPHV
                     IKAWPGTRPRQSLYVAMEFIAGQTLAQWMIDHPRPPLDAVRGIVVQLAQGLQALHRRD
                     MLHRDLRPENVMIDASGTVRLIDLASVQVGGLAEAGQADEAAPPGTVQYLAPECLTGE
                     PATPAADLYALAAIAYRMLSGELPYGLAAARVRTAPEARRLRRLPLASFRPDLPAWVD
                     AVLGKALQVDPRRRQEALSEFVRDLQQPGPQFLSTRRLPLIERDPLRFWKLLSAGLAL
                     LAILLAFGWHSAT"
     gene            complement(5965..6777)
                     /locus_tag="PLN02_00210"
     CDS             complement(5965..6777)
                     /locus_tag="PLN02_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOI50773.1"
                     /translation="MAYLAPAEFVTKMIDAGESKLLMSTRDTLIRSYMAGAILALAAA
                     FAVSVTVNTGNPLIGALLFPVGFCMLYLLGFDLLTGVFTLAPLAVLDKRPGATWNGVM
                     RNWSLVFCGNFAGAMTVAVFMAIIFTSGFSEAPNAIGQKIGHIGEGRTVGYAAHGAAG
                     MLTLFVRAVMCNWMVSTGVVAAMMSTSVSGKVIAMWMPILVFFYMGFEHSIVNMYLFP
                     SGLMLGGNFTIMDYLIWNEIPTVLGNLVGGLTFVGATLYSTHYKTAPKRAIA"
     gene            complement(6800..7138)
                     /gene="nirD"
                     /locus_tag="PLN02_00215"
     CDS             complement(6800..7138)
                     /gene="nirD"
                     /locus_tag="PLN02_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HOI50774.1"
                     /translation="MKTPEPLWLPVCGIADILPGTGVCALLGAKHIAVFRVGDDQLFA
                     LDNVDPRSGASVLSRGLTGNLGDHLVVASPLYKNHFDLRTGECLESPEHSVRTYPVQV
                     HDGLVSVSLS"
     gene            complement(7160..>9650)
                     /gene="nirB"
                     /locus_tag="PLN02_00220"
     CDS             complement(7160..>9650)
                     /gene="nirB"
                     /locus_tag="PLN02_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HOI50775.1"
                     /translation="VGHKFLEELHQLGLTGAEVTVLGEESRAAYDRVHLSEFFAGKTA
                     EDLSLVAPGFFPTSGFTLRLGSRAVAIDRRARTVSTADGQTLAYDKLVLATGSYPFVP
                     LLKGNDRPHSFVYRTLDDLEAMRASGAVSRSGIVVGGGLLGLECAKALRDLGLQTHVV
                     EYAPRLMAVQVDDDGGRVLRAKIEALGVKVHIERNTVDIRDGATARHRMVFSDGTHLE
                     ADMIVFSAGIRPRDELARNNALAVGARGGIAIDDHCRTSDPDIYAIGECAAWKDQLFG
                     LVAPGYDMARIAARHLAGDASAAFAGADMSTKLKLMGVDVASIGDAHGRTAGCRSFRY
                     VDECKQIYKKIVVSADGKHLLGAVLVGNADEYGTLLQMALNGIALPADPEFLILPAGD
                     GKAKPGLGPDALPDSAQICSCNNVSKGQIAAAVADGATTIGQIKACTKAGATCGGCVP
                     LVTQIMKAEMARRGMTVNNHLCEHFPYSRQELFHLIRVGNIRSFADLLEKHGQGLGCD
                     ICKPTAASIFASCWNDFVLQGELASLQDSNDYFLGNIQKNGTYSVVPRMPGGEVTPDG
                     LIAVGQVAKKYGLYTKVTGGQRVDLFGARVEQLPLIWEELIAAGFESGHAYGKSLRTV
                     KSCVGSTWCRYGVDDSVGLAVELEHRYKGLRAPHKIKFGVSGCTRECAEAQGKDIGVI
                     ATERGWNLYVCGNGGMKPRHAELLVADLDKATLIRLIDRFLMFYVRTADRLQRTSTWR
                     ESLEGGLDYLKSVLLDDTLGLCDELEGQMQHVVDTYQCEWKTAVSDPDVRKRFRTFIN
                     DEQADAGIVFVSERGQKRPARADECDSVSLA"
BASE COUNT         1524 a         3189 c         3439 g         1498 t
ORIGIN      
        1 tagaggcggc cgctgcggcc ggtgcggctc atgccgtgcc actggtcgcg caggcggccg
       61 gtgttgaggc gcagcgggta ggcggcatcc ggcgcttcgc cgttcaggcg cagttcgatc
      121 ggcgcgaaac gcgcccgccc gctcgccgtc gggaacacgc cgtcactgta aaggcgcgcc
      181 ttgccgttgg cttggccggc cgcgtaaggc cattgcaccg ccccctgctc gagcctggcg
      241 taatccagcc cggcaatgtc gagatcgcgg ccgcgcgtgc tgcgcgcgtg ctcggcgaaa
      301 acggcggcgg gcgacgggaa atcgaacagg cgcgcggcat cgggacggtc gagctggcgg
      361 cccagagcga gcgcgaaatc gcgcgcgatg cgccagtcgg cgcgggcttc gccgggcgcc
      421 gcaacggcag cccggacgcg ggtgatgcgg cgctcggaat tggtcaccgt gccgtccttc
      481 tcgccccagg tggcggtcgg cagcaggagg tcggcgtgcg cgcaggtatc ggtatcggcg
      541 aaggcgtcct ggacgacgac gaagggacag gcggcgagcg cttcgcgcac gcgttgctgg
      601 tcgggcagcg aatgcgccgg gttggtgcag gcgatccaca gcgccttgat ctcgccgcgc
      661 cgtaccgcct cgaacaactg cactgccatc aggccgggct gtgccgggat cgccgggatg
      721 ccccagaagt cggccatttc ggcccggtcg gccgggttct tgaggtcgcg gtgcgccgac
      781 agcaggttgg ccatggcgcc gacttcgcgc ccgcccatgg cgttcggctg gccggtcagc
      841 gagaaggggc ccatgcccgg ccggccgatc ttgccggtgg ccagatgcag gtggatcagc
      901 gccgccgaat tggccgtgcc gtgccccgac tggttcagcc cctggcacca gaacgacagc
      961 ggcgccgccg cctggccgaa ccagcgcgcc gcctgcacga tgtcggcggc cggcacctgg
     1021 cagacaccgg cggcgacctc gggcgtcacg ctgcccaact gttcgagcag ggcggcgtag
     1081 ccttcggtgt ggtcggcgat gtaggccgca tcgaccagcc cctcggcggc cagtacctgc
     1141 agcatggcgg cgaacaggta gaggtcggag cccggcgcga tctgcaggtg caggttggcc
     1201 atcgccgccg tatcggtacg gcgcggatcg acgacgacga tcttcaggtc gggccgcgcc
     1261 tcgcgcgccg cctcgatgcg gcggaacagg atgggatggg cgaaagccgt gttggcaccg
     1321 ctgatgaaga gcaggtcggc cagatcgatg tcctcgtagc agcagggcac gctgtccatg
     1381 ccgagcgtcg ccttgtagcc ggcgacggct gacgacatgc agaggcggga gttcgaatcg
     1441 atgttgttgg tgccgaccag cgcgcgcgcc agcttgttga agacgtagta gtcctcggtc
     1501 agcaactggc cggaaatgta gaaggccacc gaatccgggc catgctcgcg gatcgtctcc
     1561 gcgaaacggc gggccgcctg gtcgagcgcc tcgtcccaac ccagcgcctg cagcggcgcg
     1621 gcgcgctgct cgcgccacag cggctggacg agacggccgt cgagctggac ggtccggtgc
     1681 aggttggcgc ccttggtgca cagcttgccg aagttggccg ggtgctcggg atcgccgcgc
     1741 acgccgatga tgcggccggc gctatggctg atgacgacgc cgcagccggt gccgcagtag
     1801 gggcaggtgg agcgggtttc ctgttgcgct tcttgggtca tgcgattctc catcgatccg
     1861 ttgccgctca gccctggcgg ccggtcgcca gttgcgactc ggcatgggcc tgaccgaaca
     1921 gcaggtgatc gcgcagcggg ccgatctcgg cggccgagcg cagcaggccg aaataccagc
     1981 cgccctcggc ggcatcgccg tagagcacgg caccgcgcag gcggccgtcg cgcacgatca
     2041 gtttcttgta ctggttggcc gggcggtcga ccaggacgat ggtttcgtcg tccgggcctt
     2101 ccgtgaaatc gccggccgaa aagacgtcga tgccggtcac tttcagcttg gtcgaggtca
     2161 ccgagccgcg gtaggtggcg cttgaagtgc ccgccagatg cgcggcgcag acctgcgcct
     2221 gttcgaagag cggcgccacc agcccgtaca ccgtgccgcg atggctgacg cattcgccga
     2281 ctgcgctgat cgacaggtcg ctggtggtca gcgtgtcatc gacgacgatg gcgcgctggc
     2341 attcgagccc ggcggcctgg gcgagggcga tgttggggcg cacgccggcc gccaggacga
     2401 cgaggtcggc cggaatttcc tcgccgccgg cgaaggccac cgccgccacc cggccgtcgg
     2461 cgccggggcg gatcgccgcc gtctggcggc cgagcaggaa gcgcatgccc ttgccttgca
     2521 gcgtcgcctg cagcatttcg gccgcctggc ggtcgagctg gcgctccatg atccactggc
     2581 cgagatgcac gacgctcacc tgcatgccgc gccgggccag gccgttggcc gcttccagac
     2641 cgagcacgcc gccaccgatc accaccgcgt ggctgccgtg ctgcgtcgcg gcgatcatcg
     2701 cttcggtgtc ggcgatatcg cgatagccga tcacgccggg caggtcgttg cccggcaccg
     2761 gcggccggaa cggcgacgaa ccggtggcga tcagcagccg gtcataggcc gcgaccttgc
     2821 cgcaatcggt gtgcaccgtg cgcgtctggc ggtcgatccg tgtcacctgg cagccggcgt
     2881 gcaggacgat gccgtgttcg gcgtaccagg ccggcgggtt gagcacgatg tcggcgactt
     2941 ccatctcgcc ggcaagcacc ggcgagagca gcacccggtt gtagttgccg tgcggctcgg
     3001 cgccgaacac ggtgatctcg taacgctcgg gcgccagctt gagcagttcc tcaagggtcc
     3061 ggatgccggc catgccgttg ccgatgagaa ccagtttttc cttggccatc tgccgctcct
     3121 caagccgcca ggcgcaacgc catgtcgcag caggccgcca gatcgaccac cggaccgacg
     3181 acgatcagcg ccggcgccgg cagggccgcg gcttccaccg ccgccgcgac gtcgtcgagg
     3241 cgggccagca cttcgcgctg ggtcggctgg caaccgcggg cgatcaccgc caccggcgtg
     3301 ctcggcgcca gaccgccgcc gatcagcgct gccgagattt ccggcagcag ggcgagaccc
     3361 atgtagacga cgagcgtcgt cccggttttg gccagcgccg cccagtccgg tgccggactg
     3421 ccctgggtgc cggcgcgcgc cgtgacgaag gtgacgccgg cggccagccc gcgatgcgtc
     3481 accgggatgc cgagcgttgc cggtaccgcg atgccggcgg tcagtccgct gaccacctcg
     3541 acgcgatggc cggcgccgcg cagacgcagc atttcctcgc cgccgcgccc gaacaggaaa
     3601 ggatcgccgc ccttgaggcg gaccacgcaa tggccctggc gggcgtagcg cagcatcagg
     3661 cgctcgatga actcctgcgg tgtcgaggcc cggccaccgc gcttgccgac gtgcaggatg
     3721 cgcgcctgcg gccggaccag cgccagcact tcggccgaca ccagatcgtc ggccagcacg
     3781 acttcggctt cctgcgccag aatgcgcgcc gcacgcagcg tcagcaaatc ggccgcaccc
     3841 ggcccggcac ccacgagata gacagtcgcc atggcgttct cccggaaaaa tcgaaacaaa
     3901 aaaaggcgtc catttcccgg ccagatggtc tggccgggaa atggacgccg ttgtccatgc
     3961 cgcgccgcag tgccgtcgtc ggcaatacgt cgctggtaat tcggtgtccg ccatcatcgg
     4021 tagcggacgt cagagcatta gcaactcata tgccatcgcc ggcaatggtg cccatcccgg
     4081 cccgcctgaa acggtggaaa tcaccagccg gcggcgcatg ccggccgttc cggccggcct
     4141 ttggcggtcc cgccgcctca cccacccggc gcgccgcaac ggcactcggc gggtgcagcc
     4201 agcgcccgag cctcaccgaa tcggtgcgcc gtgctgcatc aagtcgccga gtgccagccg
     4261 aaggcgagca ggatggcgag cagggccagt ccggcactca acagcttcca gaagcgcagc
     4321 ggatcgcgtt cgatcagcgg caggcggcgc gtgctgagaa attgcggccc gggttgctgc
     4381 aagtcgcgga cgaactcgct gagcgcctcc tggcggcgtc gcgggtcgac ctgcagcgcc
     4441 ttgccgagca cggcgtcgac ccaggccggc agatcggggc ggaagctggc cagcggcaag
     4501 cgccgcagac ggcgtgcctc gggcgccgtg cggacacgtg ccgcggccag cccgtaaggc
     4561 agttcgccgc tcagcatgcg gtaggcgatg gcggccagcg cgtagaggtc ggcggccggg
     4621 gtcgccggct cgccggtcag gcattccggc gccagatact gcacggtgcc cggcggcgct
     4681 gcctcatcgg cctgcccggc ttcggccagg ccgccgacct ggacgctggc caggtcgatc
     4741 aggcggaccg tgccgctggc gtcgatcatc acgttttccg ggcgcaggtc gcggtgcagc
     4801 atgtcgcgcc ggtgcagcgc ctggaggccc tgcgccaact ggacgacgat gccgcgcacg
     4861 gcgtcgagcg gcggccgcgg atggtcgatc atccactgcg ccagggtctg gccggcgatg
     4921 aactccatcg ccacgtagag cgactggcgc ggccgcgttc caggccaggc cttgatcacg
     4981 tgcgggctgt cgacgcgccg cgccacccac tcttcgagca cgaagccgtc gagataggcg
     5041 gcgttctccc gcagatcggt agacggcagc ttgagcacga ccggcgtccg gctggcctcg
     5101 tcctcggcca gatagacatg gctgcgcgcg ctgacctgca gctcgcggac gatgctgaag
     5161 ccttcgaagc tcatccccgg ccgcagcggc ggcggcagcg gcaaggtctg gcgttgcgcc
     5221 cgtagctggt cggccgcggg tggcggcaag gcgtcgatgc gcagcaactg gacggtggcg
     5281 tcgtcgcgac tgccggccgc ccgcgccgct tcgaccaggg ttgcggcaac ggcatcgagc
     5341 gcatcgccat gctgatccag tgcggcgtgc acggcagcgg cgtcgagaaa ttcgtgcgcc
     5401 ccgtcggtag ccaacagata gatttcgccc ggttcggcgt cccagcagcg gtagtcgatc
     5461 tcgacatggc tgccgacgcc gagcgcccgg gcgaggtagc tcccggcgtc gtgccggacg
     5521 cgatggtctt cgctcaactg ttccagcgcc tgcgcgtgca ggcggtagac gcgcgcatcg
     5581 ccgacgtgca gcaggtgcag ttggcgagcc ttcaggatca gcgcgctgaa ggtgcagacg
     5641 tagccgctat cgcggtcgaa acggccagcg ccgtgcatgg tctggctgta cagccaggcg
     5701 ttgacggcat cgagcacgcg ttgcgccgag cgacggacgg tccacgcttc ggcggtggcg
     5761 taatagtcgt cgaggaaact gcgcaccgcc gtctggctgg cgatatggcc gacccggctt
     5821 gaactgatgc cgtcggcgat ggccagggcg atgcccttgg cggccagcag gtggccgccg
     5881 ggcagcagcg cgccgtggaa atcctgattg accggatgct cgccggccag cgagtgctgg
     5941 ccgagcgaga cacgcgggcc gctctcaggc gatggcacgc ttcggcgccg tcttgtagtg
     6001 cgtcgagtag agcgtggcgc cgacgaaggt caggccgccg accaggttgc cgagcacggt
     6061 cgggatttca ttccagatca ggtagtccat gatcgtgaag ttgccgccga gcatcaggcc
     6121 ggacgggaac aggtacatgt tcacgatgct gtgctcgaag cccatgtaga agaagaccag
     6181 gatcggcatc cacatggcga tcaccttgcc cgacaccgag gtcgacatca tcgccgcgac
     6241 gacgccagtc gacaccatcc agttgcacat cacggcgcgc acgaacagcg tcagcatgcc
     6301 ggcggcgccg tgcgcggcgt aaccgacggt gcggccttcg ccgatatggc cgatcttctg
     6361 cccgatggcg ttgggcgctt cggaaaagcc gctggtgaag atgatcgcca tgaacacggc
     6421 aacggtcatc gcgccggcaa agttgccgca gaagaccagc gaccagttac gcatgacacc
     6481 gttccaggta gcgcccgggc gcttgtcgag cacggcgagc ggagccagcg tgaacactcc
     6541 ggtcagcaag tcgaagccga gcaggtagag catgcagaag ccgaccggga agagtagcgc
     6601 gccgatcagc gggttgccgg tgttgaccgt gaccgacacg gcgaaggcgg cagccagcgc
     6661 gaggatggcg ccggccatgt aggaacggat caaggtgtcg cgggtggaca tcaggagttt
     6721 ggattcgcca gcgtcgatca ttttggtgac gaattcggca ggagcaaggt aggccatggt
     6781 atttacctct tgtgaaggat caggaaaggg aaacggaaac gagaccgtcg tgcacctgca
     6841 ccgggtaggt gcggaccgaa tgttccgggg attccagaca ttcgccggtg cgcaggtcga
     6901 agtggttctt gtagagcggc gaggcgacga ccaggtggtc gccgagattg ccggtcaggc
     6961 cgcgcgacag gacgctcgcc ccggagcgcg gatcgacgtt gtcgagcgcg aacagttgat
     7021 cgtcgccgac gcggaagacg gcaatgtgct tggcgccgag cagggcacag acgccggtgc
     7081 cgggcaggat gtcggcgatc ccgcagaccg gcagccaaag gggttcgggg gttttcatgg
     7141 caaagttctc cgggcaggtt caggccagcg acacgctgtc gcattcgtcg gcgcgcgccg
     7201 gccgtttctg gccgcgttcg ctgacgaaaa cgatgccggc gtcggcctgt tcgtcgttga
     7261 tgaaggtacg gaaacgcttg cggacatcgg ggtcgctgac cgccgtcttc cactcacact
     7321 ggtaggtatc gaccacgtgc tgcatctggc cttccagttc gtcgcagagg ccgagcgtgt
     7381 cgtcgagcag caccgacttc aggtagtcga gcccgccttc caggctttcg cgccaggtgc
     7441 tggtgcgctg caggcggtcg gcggtacgca cgtagaacat caggaagcgg tcgatcaggc
     7501 ggatcagcgt cgccttgtcg aggtcggcga cgagcagctc ggcgtggcgc ggcttcatgc
     7561 cgccgttgcc gcaaacgtag aggttccagc cgcgttcggt ggcgatcacg ccgatatcct
     7621 tgccctgcgc ctcggcgcac tcgcgggtgc agccggagac gccgaacttg atcttgtgcg
     7681 gcgcgcgcag gcccttgtag cggtgttcca gttcgacggc gaggccgacc gagtcatcga
     7741 cgccgtagcg gcaccaggtg ctgccgacac aggacttcac ggtgcgcagc gacttgccgt
     7801 aggcgtgacc ggattcgaaa ccggcagcga tcagttcttc ccagatcagc ggcaactgct
     7861 cgacgcgggc gccgaacagg tcgacccgct ggccgccggt gaccttggtg tacaggccgt
     7921 acttcttggc cacctggccg accgcgatca gcccgtcggg cgtcacttcg ccgcccggca
     7981 tgcgcggcac aacggagtag gtgccgtttt tctggatgtt gccgaggaag tagtcgttgc
     8041 tgtcctgcag gctggcgagt tcgccctgca gcacgaagtc gttccagcac gaggcgaaga
     8101 tcgacgccgc ggtcggcttg cagatgtcgc agccgaggcc ctggccgtgc ttttccagca
     8161 agtcggcgaa gctgcggatg ttgccgacgc ggatcaggtg gaacaactcc tgacgggagt
     8221 aggggaagtg ctcgcacaga tggttgttga ccgtcatgcc gcggcgcgcc atttccgcct
     8281 tcatgatctg cgtcaccagc ggcacgcaac cgccacaggt ggcgccggcc ttggtgcagg
     8341 ccttgatctg gccgatcgtc gtcgccccgt cggcgacggc ggcggcaatc tgccccttgc
     8401 tgacgttgtt gcaggaacag atctgcgcgc tgtcgggcag ggcgtccggg ccgaggccgg
     8461 gcttggcctt gccgtcgccg gccggcagga tcaggaattc cggatcggcc ggcagggcga
     8521 tgccgttcag cgccatctgc agcagcgtgc cgtattcgtc ggcgttgccg accagcaccg
     8581 cgccgagcag gtgcttgccg tccgcactga cgacgatctt cttgtagatc tgcttgcatt
     8641 cgtcgacgta gcggaagctg cggcaaccgg ccgtgcgacc gtgcgcgtcg ccgatgctgg
     8701 cgacgtcgac gcccatcagc ttgagcttgg tgctcatgtc ggcgccggca aaggcggcgc
     8761 tcgcatcgcc ggccagatgg cgggcggcaa tgcgcgccat gtcgtaaccc ggcgcgacca
     8821 ggccgaacaa ctggtctttc caggcggcgc attcgccgat cgcgtagatg tcggggtcgc
     8881 tcgtccggca gtggtcgtcg atggcgatgc cgccgcgcgc gccgactgcc agcgcgttgt
     8941 tgcgcgccag ctcgtcgcgc ggccggatgc cggccgagaa gacgatcatg tcggcctcga
     9001 ggtgggtgcc gtcgctgaac accatgcgat ggcgcgccgt cgcgccgtcg cggatatcga
     9061 ccgtattacg ctcgatatgg accttgacgc cgagcgcctc gatcttggcc cggaggacgc
     9121 gcccgccgtc gtcgtcgacc tgcaccgcca tcagacgcgg cgcgtattcg acgacatgcg
     9181 tctgcaggcc gaggtcgcgc aacgccttgg cgcattcgag gccgagcagg ccgccgccga
     9241 cgacgatgcc gctgcgcgag acggcgcccg aggcacgcat cgcctcgaga tcgtcgagcg
     9301 tccggtaaac gaagctgtgg gggcggtcgt tgcccttgag caggggaacg aagggatagg
     9361 agccggtggc cagcaccaac ttgtcgtagg cgagagtctg gccgtccgcc gtgctcaccg
     9421 tgcgcgcccg gcgatcgatg gcgacggccc ggctgcccag tcgcagcgtg aaaccgctgg
     9481 tcgggaagaa accgggggcg accagcgaca ggtcttcggc ggtcttgccg gcgaagaatt
     9541 cggacaggtg gacgcggtcg taggcggcgc ggctttcctc gcccagcacg gtgacttccg
     9601 cgccggtcag gccaagctga tgcagttcct cgagaaactt gtggccaacc
//
LOCUS       DAOSDX010000005         9214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2796_length_9214_cov_3.120100, whole genome shotgun sequence.
ACCESSION   DAOSDX010000005 DAOSDX010000000
VERSION     DAOSDX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2796_length_9214_cov_3.120100"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /gene="tssF"
                     /locus_tag="PLN02_00225"
     CDS             complement(<1..1568)
                     /gene="tssF"
                     /locus_tag="PLN02_00225"
                     /inference="COORDINATES: protein motif:HMM:NF017733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HOI50776.1"
                     /translation="MNPNFIDLYNEELRFLREAGQYFADAYPQVAGQLGMHEDTISDP
                     FVERLLEGVSFLTARIQARLDVEHAEFARQMLARVAPLWDESSPSIATLAFTPDFASP
                     ECQGGVTLDKGSIIQARLPGNNNVLRFTTGQTIQLLPLEISKVRCAATINHLPPRIAA
                     QAQEAAGVIALEFSCQGRAKLGELKTDFLELTLAGDIPLCHAIQKRLLHDAIKVFAWA
                     EDSGALEYIEIAKESLAIVGVTPEESLLPSSASMLPGLRLLREYFAQPSRFLGVRISG
                     LAKLFKRIGEHRKFHIGFLLKSEPANIINRINAQCFRLFATPVINLYPKTFDPIVYNP
                     DRTDQWLLVDRLRPLAYDIHHVTDCRAILENGRCLGLAQVPPRNGYAQDGQEGHFSSR
                     KQYVTYGSVDKGSLKNQLPIRHFVNIGLNPDTGVPEDVKSLQIDGYVSDRGWYREHLD
                     QAGFALTSSSQIGKIECLRLPSEARDEPLPERHWEAVQLLGSNPLRMRSRDDSNLVNA
                     LHLWLGLASDPAVLA"
     gene            complement(1565..2035)
                     /locus_tag="PLN02_00230"
     CDS             complement(1565..2035)
                     /locus_tag="PLN02_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOI50777.1"
                     /translation="MKPHSQIKHSYTPNLFQRISQGAGSHASVADSIQEEVLQLLRHA
                     ARSRRPRVDSEAQICSSVFNYGLPMASFGSTSVSDVEAVVRHLKRMIPVFEPRIDPHS
                     LQITTTLSNDQHFRESVLFDISAEGKGNVVAYLSFRLAVDFSHGAVRLVRRPNL"
     gene            complement(2091..2615)
                     /locus_tag="PLN02_00235"
     CDS             complement(2091..2615)
                     /locus_tag="PLN02_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HOI50778.1"
                     /translation="MAQALVDYFLQIDGVEGESPDQQYPGLIQLQSWQWAEENSGRWG
                     FGSGGGAGKVEMKDFEFRMVTNKASPKLFLMCATGDHIPQAKLICRKSGGTQLDFMTV
                     TFSNCLVSSFTTVGNLPGDMATKFGVTETVLPVDEIKINFARIEVEYKEQRNDGTMGA
                     VIKAGYDLKLNATV"
     gene            complement(2694..4199)
                     /gene="tssC"
                     /locus_tag="PLN02_00240"
     CDS             complement(2694..4199)
                     /gene="tssC"
                     /locus_tag="PLN02_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HOI50779.1"
                     /translation="MSTVFESQADATQVVTLENDSELKELLKKSFRPRTNEAAEAVQT
                     AVNTLLQFVRRDKVVVYKDVLKTVDELVAEIDAKISKQLTKILHHPEMQKLEGAWRGL
                     HYLVSNTEVSANLRIRVMNISKKELGRTLRHYKGGAWDQSPIFKKIYEQEFGQFGGQP
                     FGCIVGDYEFNHSPHDVALLTEMAKIAASAHAPFISAAAPGTMQMDSWSELANPRDIS
                     KIFQAVEYAAWRRLRESNDSRYLSLTLPRFLARLPYGAHTHEVPEFAFEETTNPDSDK
                     DFCWANSAYAMAANITRAFSEYGWCTKIRGVESGGAVEILPNFVYSNSDGEKLLYCPT
                     EIAISDRREHELSNAGFMPLVFRKNSDMAAFIGARTMHKPAIYEDDDATANANLSARL
                     PYIFATCRFAHYLKCIVRDKIGSFKSREETQRWLNDWIMNYVDGDPTISSEATKSRRP
                     LSAAEVIVDELPDNPGYYRAQFFLRPHYQLEGLTVSLRLVSKLPSAKQTSQ"
     gene            complement(4217..4783)
                     /gene="tssB"
                     /locus_tag="PLN02_00245"
     CDS             complement(4217..4783)
                     /gene="tssB"
                     /locus_tag="PLN02_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HOI50780.1"
                     /translation="MAKHYDGQKFIGKQRAPRVQIEYDVEIYGSQKKVELPFVVGVMA
                     DLSGENRESLAPIEERDFIDIDDETFDKRMAQIAPKVAFYCEDTLTDDDPGALIDVNL
                     TFNSMQDFEPGEIARRIPELAALLKARNELKELTTYMDGKMAAQDLIEQILTNTKYTG
                     KELEVRDDADWPKAPEEDLPAAQDKQPE"
     gene            complement(4815..5804)
                     /locus_tag="PLN02_00250"
     CDS             complement(4815..5804)
                     /locus_tag="PLN02_00250"
                     /inference="COORDINATES: protein motif:HMM:NF018516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HOI50781.1"
                     /translation="MAGEHSNIDLEFDERFLIIDQLISEHEADFDAPLRKGKDPFDWA
                     RIADDCAALARETRDFRIALWWLRARAEVGGLVAWVDGLSYILELLELPEDAVHPQGD
                     AELSSGELHATALNWLTSSRSLAAFRKLPLTSDAAWSVAALLPDVAGSRHGSDSEQAK
                     LRDALRADQARSDLNCALVNRAKACLEEIAVRLDERAGDAGGDFSELIAAVGHAAHRL
                     APLTTHSQQLEAVAEAAAAEAAAGEPVADAPSAVGGMRLAGDGFAIHSRDEVRLVLTA
                     LERYYNEHESGHPAPIFIQRLQRMVDMSFELLMRELFSESDRLLDRLVKPAAN"
     gene            6145..7407
                     /gene="lplT"
                     /locus_tag="PLN02_00255"
     CDS             6145..7407
                     /gene="lplT"
                     /locus_tag="PLN02_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HOI50782.1"
                     /translation="MPFGFYIIMAAQFFSALADNALLIAAIAALREMHAPEAYEPLLK
                     TSFTLSYVVLAAFVGAFADSMPKGRVMLISNSIKIVGCALMFFHVHPLFAYAIVGLGA
                     AAYSPAKYGILTEYLPHRLLVVANGWIEGLTVGAIILGVVIGGLLIEPKIAAHLLVFD
                     LPFIDTGLDTAGEVAITLIILLYVIAALFNLYVPDTGVDHKPLKKNPFYLLHEFGHCL
                     SLLWRDRLGQISLAVTTLFWGAGATLQFIVIKWSAVALGLDLSASSMLQGVVAFGIAG
                     GAIVAARFITLRKSLGVIPLGIAMGIVVLVMNFVHHIWLAIPLLILIGGLSGFFVVPM
                     NALLQHRGHILMGAGHSIAVQNFNENLSILIMTGLYYVMIRADLSIYWILTLFGLSVS
                     GLMYLIRKRHLANQRDRDDVIHLDDSAH"
     gene            7602..8075
                     /locus_tag="PLN02_00260"
     CDS             7602..8075
                     /locus_tag="PLN02_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50783.1"
                     /translation="MAVYKGIEIDDALTGSMRHMQGVEDYREVLGKLQAAWDTLALLG
                     QLTGAATEMTGTREAFERLTGDLLNRLGQETRRKAIAELRAKAQTSIDILVRNLFERT
                     ADIGFLAADDDIREFLQGAGETREALEARFHEYVQKYSSMPTSSSSAPTAGSPPA"
     gene            8075..>9214
                     /locus_tag="PLN02_00265"
     CDS             8075..>9214
                     /locus_tag="PLN02_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50784.1"
                     /translation="MPGSCEASAHPLLDDARSTTSAYVEYYGPADFLAPGQHLLYAYR
                     VEDAAGECLGILALHFRLEDEMRSIFANLTGTGDSCLLANFDSGGLVVATSSPLQLPA
                     GTQLDDSLRQARGDVVRLAGREYLAIACAAHPYQGYPGPGWCGLAMVPLEFAFERRDS
                     ELLKHIDSAILQSVAHQPKLFNDELRLIPQQAERIQEDLNRSVWNGSVRQVDNNSGNA
                     AFAKTLLWEISSTGRKTQAVFEQSIGNLHETVVAALLQNGISRAAFAIDVMDRNLYER
                     ANDCRWWALNATFRRVLSEPRVSPEDAVRCGEILRYINGLYTVYANLILYDAHGQVIA
                     VSDSEQASLCGTRLHEEWVGSSLALDNSQGYVCSHFAPTPLYGNRH"
BASE COUNT         1770 a         2838 c         2747 g         1859 t
ORIGIN      
        1 gcgagcacgg ccggatcgct ggccagcccc agccacagat gcagggcatt gaccagattg
       61 ctgtcgtccc ggctgcgcat gcgcaaggga ttgctgccaa gcagttgcac cgcctcccag
      121 tgccgctcgg gaagcggctc gtcgcgcgct tcgctgggca ggcgcaggca ttcgatcttg
      181 ccgatctggc tcgaactggt cagcgcaaac cctgcctggt cgagatgctc ccgataccag
      241 ccgcgatcgc tgacgtaacc gtcgatctgc aacgacttca catcctccgg aacgccggtg
      301 tccggattga ggccgatatt gacgaaatgg cggatcggca gttggttctt caacgagccc
      361 ttgtcgaccg aaccgtaggt gacgtactgc ttgcgcgaac tgaaatgtcc ttcctggccg
      421 tcctgcgcat aaccgttgcg cggcggcacc tgggccagac cgaggcagcg cccgttttcc
      481 aggatggcgc gacaatcggt aacgtggtgg atgtcgtaag ccagcggacg cagacgatcg
      541 accagcagcc actgatcggt gcgatccggg ttgtagacga tggggtcgaa ggtcttcgga
      601 tagagattga tgaccggcgt cgcgaacagg cggaaacact gcgcgttgat ccggttgatg
      661 atgttggccg gctcgctctt cagcaggaaa ccgatatgga atttgcgatg ctcgccgatg
      721 cgcttgaaca acttggccag cccggaaatc cggacgccga ggaagcggct cggctgggca
      781 aaatactcgc gcagcaggcg caggccaggg agcatgctgg ccgacgaggg cagcaggctt
      841 tcttccggcg tgacgccgac gatggccagc gattccttgg cgatttcgat gtattccagc
      901 gccccgctgt cctcggccca agcgaagacc ttgatcgcat cgtgcagcaa gcgcttctgg
      961 atggcatggc agagaggaat gtcgccagcc agggtcagtt cgagaaaatc ggttttcagc
     1021 tcgccgagct tggcccggcc ctggcaggaa aattccagcg cgatgacgcc ggcggcttcc
     1081 tgggcctgtg ccgcgatacg cggcggcagg tgattgatgg tggcggcgca acgcaccttg
     1141 gagatttcca gtggcagtaa ctggatcgtc tgcccggtgg tgaaacgcag cacgttgttg
     1201 ttgcccggca ggcgggcctg gatgatgctg cccttgtcca gcgtgacgcc gccctgacac
     1261 tcaggcgagg caaaatccgg ggtgaaggcc agggtggcga tcgaaggcga cgattcatcc
     1321 cacaggggag cgacgcgggc cagcatctgg cgcgcgaact cggcatgctc gacatcgagc
     1381 ctggcctgga tgcgggcggt caggaaggaa accccttcca gcaggcgctc gacgaaaggg
     1441 tcgctgatcg tgtcctcgtg catccccagt tgcccggcga cctggggata ggcatcggcg
     1501 aagtattgcc cggcttcgcg caggaaacgc agttcttcgt tatagaggtc gatgaagttc
     1561 ggattcacaa attcggcctg cggacgagac gaaccgcgcc gtgactgaaa tcgacggcaa
     1621 ggcgaaacga gagataggca acgacgttcc ccttcccttc ggcgctgatg tcgaacagga
     1681 ccgattcgcg gaagtgctgg tcattggaaa gggtggtggt gatctgcagc gagtgcggat
     1741 cgatccgcgg ttcgaagacc ggaatcatcc gcttgagatg ccggacgact gcctcgacat
     1801 cggaaacact ggtgctaccg aaggaagcca tcggcaagcc gtagttgaat accgacgaac
     1861 agatctgcgc ttcactgtcg acgcgcgggc gacgactacg cgccgcatga cgcaggagct
     1921 gcagcacctc ttcctggatg gaatcagcga ccgaggcgtg actccccgcc ccctgactga
     1981 tccgctggaa caggttgggc gtgtaggagt gcttgatctg gctgtgtggt ttcatgtgct
     2041 gttttggtgc cgacgaaccc tgttcgccag caccaaatgc cgactgaggt ttagaccgtt
     2101 gcgttcagct tcaggtcata gcccgccttg atgacggcgc ccatcgtgcc gtcattgcgt
     2161 tgctccttgt actcgacttc gatgcgggcg aagttgatct tgatttcgtc aaccggcagc
     2221 accgtttcgg tcacgccgaa cttggtggcc atgtcgcccg gcaggttgcc gacggtggtg
     2281 aaagacgaaa ccaggcagtt gctgaaggtc accgtcatga agtcgagctg cgtgccaccg
     2341 gacttgcggc agatcagctt ggcttgcgga atgtgatcgc cggtcgcgca catcaggaag
     2401 agcttcggcg aggccttgtt ggtgaccatg cggaactcga agtccttcat ttcgaccttg
     2461 cctgcgccac caccgctacc gaaaccccaa cgaccggagt tttcttcagc ccactgccag
     2521 ctttgcagct ggatcaagcc aggatactgc tggtcgggag actcgccctc gacgccgtcg
     2581 atctgcagga aatagtcaac aagtgcttgt gccatgattg ctcctaggaa aagatgaatg
     2641 gaaccgtgga atgaccgggc ggactagaat ttgtcagtac gcccggcgtt tcctcactga
     2701 ctcgtctgct tggcggacgg cagcttggaa accagccgga gggacacggt caacccctca
     2761 agttggtaat gcggacgcag gaagaactgg gcgcggtagt aacccgggtt gtcagggagt
     2821 tcgtccacga tcacttcagc agcggacagc ggccggcgcg atttcgtcgc ttcgctcgag
     2881 atcgtcggat cgccatcgac gtaattcatg atccagtcgt tgagccagcg ttgcgtttct
     2941 tcgcgcgact tgaacgaacc gatcttgtcg cggacgatgc acttcaggta atgggcgaag
     3001 cggcaggtgg cgaagatgta gggcagccgg gccgacagat tggcgttggc ggtggcgtcg
     3061 tcgtcttcgt agatggccgg cttgtgcatg gtgcgggcgc cgatgaaggc cgccatgtcc
     3121 gaattcttgc ggaaaaccag cggcatgaag ccggcgttcg acagttcgtg ttcgcgacgg
     3181 tcggaaatcg cgatctcggt cgggcaatac agcagcttct cgccgtccga attggaatag
     3241 acgaagttcg gcaggatttc gacggcaccg cccgattcga cgccgcgaat cttggtgcac
     3301 cagccgtatt cggagaaggc ccgggtgatg ttggcggcca tcgcgtaagc cgagttggcc
     3361 cagcagaaat ccttgtcgct atcgggattg gtggtttcct cgaaagcgaa ttccggtacc
     3421 tcgtgggtgt gcgcaccgta gggcaaacgc gccaggaaac gcggcaaggt cagcgacagg
     3481 tagcgcgagt cgttcgattc gcgcagacgg cgccaggcgg catattcgac ggcctggaag
     3541 atcttcgaga tgtcgcgcgg gttggccagt tccgaccagg agtccatctg catggtgccc
     3601 ggtgccgccg ccgaaatgaa gggggcgtgc gcggaagcag cgattttcgc catttcggtg
     3661 agcaaggcga cgtcgtgcgg gctgtgattg aattcgtagt cgccgacgat gcagccgaaa
     3721 ggctggccgc cgaactggcc gaattcctgt tcgtagatct tcttgaagat cgggctctgg
     3781 tcccaggcgc cgcccttgta gtggcgcagg gtgcgaccga gttccttctt gctgatgttc
     3841 atcacccgga tgcgcagatt ggcgctgacc tcggtattcg agaccaggta atgcaagccg
     3901 cgccaggcgc cttccagttt ctgcatctcg gggtgatgca ggatcttggt cagttgcttg
     3961 gaaatcttgg cgtcaatctc ggcgaccagt tcatcgaccg tcttcagcac gtccttgtag
     4021 acgacaacct tgtcgcggcg gacgaactgc aacaaggtgt taactgcggt ctgcaccgct
     4081 tcggcggcct cgttggtgcg gggacggaag gatttcttca acagctcctt gagttcgctg
     4141 tcattctcca gggtgacgac ctgggttgca tcggcttggc tttcgaatac ggtactcatc
     4201 gcgttcactc tttcgttcac tcgggttgct tgtcttgagc agcaggcagg tcctcttccg
     4261 gtgccttcgg ccaatcggcg tcatcgcgga cttccagttc cttgccggtg tacttggtgt
     4321 tggtcaggat ctgctcgatc aaatcctggg cggccatctt gccgtccatg taagtggtca
     4381 attccttcag ctcgttacgc gccttgagca gcgcagccag ctccggaatg cggcgggcga
     4441 tctcgccggg ctcgaagtcc tgcatcgagt tgaaggtcag attgacgtcg atcagcgcac
     4501 cgggatcgtc gtcggtcagc gtgtcttcgc agtagaaggc aaccttcggc gcgatctgcg
     4561 ccatccgctt gtcaaaggtt tcatcgtcga tgtcgatgaa gtcccgctcc tcgatcggcg
     4621 ccagggattc gcggttttca ccggaaaggt ccgccatcac ccccaccaca aaaggcagct
     4681 cgactttctt ctgcgagcca tagatttcaa cgtcgtactc gatctgcacc cgcggggcgc
     4741 gttgctttcc gatgaatttc tgaccgtcgt aatgtttagc cacacctgct ctccgtcgat
     4801 aaaaaggtga aaaatcaatt cgcagccggt ttcaccagcc gatccagaag cctgtccgat
     4861 tcggaaaaca actcccgcat cagcagctca aaactcatgt cgaccatccg ctgcaggcgt
     4921 tggatgaaga tcggcgccgg atgaccgctt tcgtgttcgt tgtaatagcg ttcgagcgcc
     4981 gtcagtacga ggcgcacttc gtcgcggcta tggatggcaa agccatcgcc tgccagacgc
     5041 atgccgccga cggcgcttgg cgcgtcggca acgggttctc cggctgcggc ttcggcagca
     5101 gcggcttcag cgaccgcttc aagctgctgc gaatgcgtcg tcagcggcgc cagacggtgc
     5161 gcggcgtgac cgacggcagc gatcaattcg ctgaagtcgc caccggcatc gccggcccgc
     5221 tcgtcgagac ggaccgcgat ttcttccaga caggccttgg cccggttgac caaggcgcaa
     5281 ttgagatcac tgcgcgcctg atcggcgcgc aaggcatcgc gaagctttgc ctgttcgctg
     5341 tccgaaccat ggcgcgagcc agcgacatcg ggcagcaggg cggcaacgct ccaggcggcg
     5401 tcgctggtca gggggagttt ccggaaggcg gccagactgc gggaggaagt cagccagttg
     5461 agtgccgtcg catgcagttc gccgctcgac agttcagcat cgccttgagg atggacagcg
     5521 tcctccggga gttcgagcaa ttcaaggatg tagctcaggc catcgaccca ggcgaccaga
     5581 ccgccgactt cggcgcgcgc ccgcaaccac cagagcgcaa tacgaaaatc ccgggtctcg
     5641 cgcgccagcg ctgcgcaatc gtcggcgatc cgcgcccagt caaaggggtc tttccccttg
     5701 cgtaatggcg catcgaagtc cgcctcgtgc tcgctgatca actgatcgat gatcagaaag
     5761 cgctcatcga actcgagatc gatatttgaa tgttcccctg ccaacaattc cagcccctgg
     5821 ttgctacaaa gtctctgttt ctgccggggg gcttatttat gcttttttaa tatgcttaat
     5881 aattgcggcc aatatatcaa cagcattttg taatggtgtt tagatttgta aacctggagc
     5941 aacagagcgt ttacaaattt ctacactgaa aatattcaca tgtgataaca gcgcggacat
     6001 acaacaggag tgtctgtttg tgcgcagtaa tccgcgcgaa aaaatcgccc cgatccgctc
     6061 gccaaggaaa acaccggaca cgaacgccct tgatcaatac gtggtataaa cctcgcccaa
     6121 agtaatacga gagagatcag ttccgtgccg ttcggcttct acatcatcat ggctgcgcaa
     6181 tttttctctg cgctggccga caatgcgctg ctcattgccg ctatcgcggc attgcgtgaa
     6241 atgcatgccc cggaagccta cgaaccctta ctgaaaacca gcttcaccct ctcctacgtc
     6301 gtactcgccg cctttgtcgg cgcctttgcg gattcgatgc cgaaaggacg ggtgatgctg
     6361 atcagcaaca gcatcaagat tgtcggttgc gcgctgatgt tcttccatgt ccatccactg
     6421 ttcgcctacg ccatcgtcgg cctgggcgcc gccgcctact cgccggcgaa gtacggcatc
     6481 ctcaccgaat acctgccgca tcgcctgctg gtcgtcgcca acggctggat cgaaggcctc
     6541 accgtcggcg ccatcatcct tggtgtcgtc attggcggac tcctgatcga gcccaaaatt
     6601 gccgcgcacc tgctggtgtt cgatctaccg ttcatcgaca ccgggctcga cactgccggc
     6661 gaagtagcca tcacgctgat catcctgctg tatgtgatcg ccgccctgtt caacctctac
     6721 gtccccgata ccggcgtcga tcacaaaccg ctgaagaaga accccttcta tctgctccac
     6781 gaattcggcc actgcctgag cctgctctgg cgtgatcgcc tgggccagat ttcgctggcg
     6841 gtaacgacct tgttctgggg agcgggcgcg acactgcagt tcatcgtcat caaatggtcg
     6901 gcggttgcgc tcggcctcga cctttcggca tcgtcgatgc tgcagggcgt ggtcgccttc
     6961 gggattgccg gcggggcgat cgtcgccgcc cgattcatca ccttgcgcaa gtcgctcggg
     7021 gtcattccgc ttggcatcgc catgggcatc gtcgtgctgg tcatgaactt cgtccaccac
     7081 atctggctgg ccattccgtt gctgatcctg atcggcggcc tttccggttt cttcgtcgtg
     7141 cctatgaacg ccctgttgca gcaccgcggc cacatcctga tgggtgccgg gcattcgatc
     7201 gccgtccaga acttcaacga gaacctgtcg atcctgatca tgaccggtct ctattacgtg
     7261 atgattcgcg ccgacctgtc gatctactgg atcctcactc tgttcggtct gtcggtcagt
     7321 ggcctcatgt acctgatccg caagcgccac ctggcgaacc agcgcgaccg cgacgacgtc
     7381 atccatctcg acgattcggc gcactgaccg accgccttgg caggcttgct acattgtcgg
     7441 tccgcaagcc gacaacatcg cccttcctcc ctgcaaaccc cttgccccgc aagcctttga
     7501 aaaaaggaac gttttttgct tagtcgaaca atcggcgacg ccgcagccag atcggcgacg
     7561 gtttcagagc aaaaatccaa tattcgggca ggggacaaga catggcagtt tataaaggca
     7621 tcgagatcga cgatgcgctg accggcagca tgcgccacat gcagggcgtc gaggattacc
     7681 gggaagtgct gggtaaattg caggctgcct gggacaccct cgccctgctc ggccagctga
     7741 ccggcgccgc caccgaaatg accggcaccc gcgaagcctt cgagcgcctc accggcgatc
     7801 tcctcaaccg cctcgggcag gaaacccggc gcaaggcgat cgccgaactg cgcgccaagg
     7861 cgcagaccag catcgacatc ctggtccgca acctgttcga acgcactgcc gacatcggct
     7921 tcctggccgc cgacgacgac atccgggaat tccttcaggg cgccggcgaa actcgcgaag
     7981 cgctggaagc acgctttcac gaatacgtac aaaaatattc ctctatgccg acatcgtcct
     8041 cttccgcgcc gacggccgga tcgccgcccg cctgatgccc ggtagctgcg aggccagcgc
     8101 gcaccccttg ctcgacgatg ccaggtcgac gaccagcgct tacgtcgaat actacggccc
     8161 ggccgacttc ctcgcccccg gtcagcacct gctctatgcc taccgcgtcg aggacgccgc
     8221 cggggaatgc ctcggcatcc tggccctgca tttccgtctg gaagacgaga tgcgcagcat
     8281 cttcgccaat ctgaccggca ccggcgacag ttgcctgctg gccaacttcg acagcggcgg
     8341 cctggtggtc gcgacatctt cgcccctgca gctaccggcc ggcacgcaac tggatgacag
     8401 cctgcgccag gcccgtggcg acgtcgtccg cctcgccggc cgcgaatacc tggcgatcgc
     8461 ctgcgccgcc cacccttacc agggctatcc cggccccggc tggtgcgggc tggcgatggt
     8521 cccgctcgag tttgccttcg agcgacgcga cagcgagcta ctcaagcata tcgacagcgc
     8581 catactgcaa tccgttgccc accagcccaa gttgttcaac gacgaactgc gcttgatccc
     8641 gcaacaggcc gaacgcatcc aggaagacct caaccgttcg gtgtggaacg gatcggtccg
     8701 ccaggtggat aacaacagcg gcaacgccgc cttcgccaag accctgctct gggaaatctc
     8761 cagcaccggc cgcaagaccc aggcagtgtt cgaacaatcg atcggcaacc tgcacgaaac
     8821 cgtcgtcgcc gcgctgctcc agaacggcat ctcgcgcgcc gccttcgcca tcgacgtgat
     8881 ggaccgcaat ctctacgaac gcgccaacga ctgccgctgg tgggcgctca atgcaacctt
     8941 ccggcgagtt ctgagcgaac cccgcgtcag cccggaagat gccgttcgct gcggcgaaat
     9001 cctgcgctac atcaacggtc tgtacacggt ttacgccaat ctcatcctct acgacgccca
     9061 cggccaggtc atcgccgttt ccgacagcga gcaggcaagc ctgtgcggca cccgcctgca
     9121 cgaggaatgg gtcggtagca gcctcgccct cgacaactcg cagggctacg tctgctcgca
     9181 tttcgcgccg acgccgctgt acggcaaccg gcat
//
LOCUS       DAOSDX010000006         9208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2802_length_9208_cov_3.878291, whole genome shotgun sequence.
ACCESSION   DAOSDX010000006 DAOSDX010000000
VERSION     DAOSDX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2802_length_9208_cov_3.878291"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="fusA"
                     /locus_tag="PLN02_00270"
     CDS             <1..532
                     /gene="fusA"
                     /locus_tag="PLN02_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285991.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOI50785.1"
                     /translation="EFVDQVKGGVIPGVFMPAVEKGVRQALAGGVVAGYEVQDLRVTV
                     YDGKTHPVDGKEVAFTIAGRKAVIEAIRNARPIVLEPIVSIEIVAPENAIGDLAGDLS
                     GRRGHVTGTDGRGHGFAAISGEVPLAELDDYQSRLKSLTGGQGSYTIDFARYAAVPPT
                     VQQQLASRFQLHDEEE"
     gene            complement(582..1316)
                     /locus_tag="PLN02_00275"
     CDS             complement(582..1316)
                     /locus_tag="PLN02_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI50786.1"
                     /translation="MKEQDSGKLIAIVEDDPDVARIIEQVLADFGFRTLWCRSGGDLL
                     RRLRNLSPALCIIDLGLPDMDGIEAMQRVRAQTACGIMILTGRAHVSDRVMGLELGAD
                     DYVLKPFEPRELVARVRSILRRRESASEQTPSQIAHFGGWRFNLANNTLGAPDGNEQV
                     LSTAEAELLKVFVSHPNRILQREKLMGTRDVGPSDRAIDVRVSRLRRKLEPDADSPSF
                     IKTVYGAGYLFLAAVEWHEGDAGSGH"
     gene            complement(1313..3343)
                     /locus_tag="PLN02_00280"
     CDS             complement(1313..3343)
                     /locus_tag="PLN02_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HOI50787.1"
                     /translation="MPHASLPADQSNRLATLQDGLDLLDQGISVFDRDLRLVAWNRPF
                     LELLGFPNELGRIGTPFADFIRYNAQRGEYGPGDPEAQVAERVAEARHFVAHVSERRR
                     PNGRLLLLRGEPLPHGGFVTLYSDITEQRYLEQLSEHQNLQLDERVRRRTAQLENANA
                     NLRQANAEKERIAAALGRSEARLRLINDTIPILIGYVDRQQIYQYANKGYADWYCLPA
                     GSVIGRSVLEVVGPEVYVQVGEHVRRALSGEQISYEYRMLRHGQTVFAHSTLVPEIGS
                     DGDTHGFFVFSHDITEQKRMQSALLQAQKMEAIGRLTGGLAHDFNNLLTVIIGNLAAL
                     QDHRPDDPGIADFVSPALQSARRGVQLIRRLLTYSRQQPLEPQAVDIGQLIGDLARLI
                     RRSLPDNIVVDCRNAEGGLFAKVDPAQLESALLNFALNARDAMPQGGRLTISADLTEL
                     PADAPETAEFDVAPGNYVAIAVADDGCGMDAHTLAHACEPFFTTKRLGLGNGLGLAMA
                     QGFIRQSAGGMRIRSEPERGTQVEIVLPQTAAPASRQAVAETPLPEASGDLVLLVEDD
                     ENVRRVVRQQLIDLGHPVIEAENASQAHDMVRHIPDIAIVVSDVLMPGELNGRQLARL
                     LRRECPSMRIVLMSGYTEDEDARGRDFPILAKPFLRQELARALQRAAGEETP"
     gene            3630..5408
                     /locus_tag="PLN02_00285"
     CDS             3630..5408
                     /locus_tag="PLN02_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOI50788.1"
                     /translation="MSTYTAPLRDMQFVLKDIAGLDEICALPGNEECSVDLVESILEE
                     AAKFASGVLDPINRNGDNQGAVVKDGVVTTSPGFRDAYQLFCETGWNAMPFSPEYGGQ
                     GLPAAVTMAVNEMWKSANMAFALCPMLTGGAIEAIAHHASDELKQKYLPKMVEGVWTG
                     TMNLTEPNAGSDLAAISTKARKLDDGTYLVTGTKIFITWGENDCAENIIHLVLARLPD
                     APPGLKGISLFLVPKFLVNDDGSLGARNDLICASLEHKLGIHGSPTAVMSYGDQEGAV
                     GYLIGEENKGIGYMFTMMNHARVNVGLEGVGIAERAYQHALWYAKERIQGAIVGDKSG
                     EKKPILHHPDVRRLLMDVKSRTEAMRTLAYYAAAQIDKAHAGDSAAQARIDLLTPVVK
                     GWSTEQGVELSSNALQVFGGVGFIEETGAAQYYRDSRITTIYEGTTAIQANDLVGRKL
                     AREKVPGAGMKAMLAEINATADELAGNAGLANIAANLKNGAAALTAAADWILANYNDK
                     PQAVHAGSVPFLKLTGIVAGGWLMAKSAGVAAARIATGDSDPFYRAKLATAEYFATHQ
                     LPFAAAYAAEVMGGAEAVFGLAEDLF"
     gene            5417..7054
                     /locus_tag="PLN02_00290"
     CDS             5417..7054
                     /locus_tag="PLN02_00290"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HOI50789.1"
                     /translation="MRTDRDAMEYDVVIVGGGPAGLTAAIRVKQQAAAAGRELSVCLL
                     EKGSEIGAHILSGAVIETRALAELFPDWQERGAPLNTPAGEDRFLFLTEGGSFKLPTP
                     PQMANHGNYIVSLGNFCRWLGEQAEALGVEIYPGFAAAEVLYHEDGSVKGVATGDLGN
                     HKDGQPGHNFQPGMELHARQTIFAEGCRGSLTRELFERFNLRDGADPQTYGIGIKELW
                     EIDPAKHQPGLIVHSVGWPLANDTYGGSFLYHLENNLVAVGFVVGLDYKNPWLSPFEE
                     FQRYKTHPAIRGFFEGGRRISYGARALSEGGFQSLPRLTFPGGLLVGDTAGFLNVPKI
                     KGTHMAMQSAMLAADAVVALLADAEGGREAQAYPLALRDNWLWDELHRVRNVRPAFKW
                     GLWGGLAYGALDTFLFRGKAPWTLAHHDDHSQLGEKSAYAKIDYPKPDGKLTFDRLSS
                     VFLSATNHEENQPSHLRLKDATVPVSINYAKYGAPETRYCPAGVYEILGEDEGAPRLQ
                     INAQNCLHCKTCDIKDPTQNIVWTVPEGGGGPNYPNM"
     gene            7278..8312
                     /locus_tag="PLN02_00295"
     CDS             7278..8312
                     /locus_tag="PLN02_00295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOI50790.1"
                     /translation="MLGKLGVMTSIRDTELLEQSLLRTLGPLLGVLDTSLYRLDEAQL
                     LVRVIHYHRSKVVDGDGVARMLERIEEIVQLSDVAPEVLALTDNVRLLGKPCTRVLEG
                     GMLIAYPLLGAGEVCGYFVFCRDHEVSANEDATIRGVLEVFSNYYALLDVSQRDRLTG
                     LFNRQALENNFERIWSAMAQPETFHERQDGRRRHGQGKYWLAVIDIDHFKRINDTFGH
                     MVGDEILLLVSRLMQNTFRSSDLLYRYGGEEFVAIVAAENEAIARNVFERVRLAIEAH
                     SFPRIDRLSVSIGYAEVDPEILPVEVLSRADRSLYQAKQDGRNRVYDFQELVASGIFP
                     SPSYGDAELF"
     gene            complement(8309..9196)
                     /locus_tag="PLN02_00300"
     CDS             complement(8309..9196)
                     /locus_tag="PLN02_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI50791.1"
                     /translation="MEALVAERTGALEQANRQLEREQAELTALLHKVEEAQQQLLQSE
                     KMAAIGQLAAGVAHEINNPIGFVNSNLSSLKHYVEQLLSVIAAYEGGNAEAIGAARRA
                     ADLDFLREDLPALLAESQEGLGRVTRIVQDLKDFSRVDQTERQHADLNAAMQSTLNVV
                     RNELKYKADVILELGEIPEVDCVPAQINQVFMNLLVNAAQAIQEHGRITVRSGVAGER
                     VWFEVEDTGRGMSDAVRQRIFEPFFTTKPVGKGTGLGLSISYDIIVKKHRGYIDVDSE
                     PGKGSRFRIWLPIKAAAPA"
BASE COUNT         1555 a         2972 c         3036 g         1645 t
ORIGIN      
        1 cgaattcgtc gaccaggtca agggcggcgt gattcccggc gtcttcatgc cggcagtcga
       61 gaagggcgtg cgccaggcgc tggccggcgg cgtcgtcgcc ggctacgaag tgcaggatct
      121 gcgcgtcacc gtctacgatg gcaagaccca tccggtcgac ggcaaggaag tcgccttcac
      181 catcgccggt cgcaaggcgg tgatcgaggc gatccgcaac gcccggccga tcgtcctcga
      241 accgatcgtc agcatcgaga tcgttgcccc ggaaaatgcc atcggcgacc tcgccggcga
      301 tctctccggg cgccgcggtc acgtcaccgg taccgacggt cgcggtcacg gcttcgccgc
      361 gatcagcggc gaggtgccgc tggccgagct cgacgattac cagtcgcggc tgaagtcgct
      421 gaccggcggc cagggcagct acacgatcga cttcgcgcgc tacgcggcag tgccacccac
      481 cgtccagcag caactggcga gccgcttcca gctccacgac gaagaagagt aagcgcctgc
      541 ttccgctgtt tgccaagggc cgcgttgcgg cccttcgcct ttcagtggcc gctgccggcg
      601 tcgccctcgt gccactcgac ggcggccagg aagaggtagc cggcgccgta caccgtcttg
      661 atgaaggagg ggctgtcggc gtccggttcg agcttgcgcc gcaggcggga gacgcggacg
      721 tcgatggcgc ggtcgctggg gccgacgtcg cgcgttccca tcagtttttc gcgctgcagg
      781 atgcggttgg ggtggctgac gaagaccttg agcagttcgg cttccgccgt gctcagtacc
      841 tgttcgttgc cgtccggcgc gccgagcgtg ttgttcgcca ggttgaaacg ccagccgccg
      901 aaatgggcga tctggctggg cgtctgttcg ctggcgcttt cgcggcggcg caggatgctg
      961 cgcacgcggg cgaccagttc gcgcggttcg aacggtttca gcacgtagtc gtcggcgccc
     1021 agttcaaggc ccatgacgcg gtcgctgacg tgggcgcggc cggtcaggat catgatgccg
     1081 caggcggtct gtgcgcgcac ccgctgcatg gcctcgatac cgtccatgtc gggcaggccg
     1141 aggtcgatga tgcacagcgc cggcgacagg ttgcgcaggc ggcgcagcag gtcgccgccg
     1201 ctgcggcacc acagcgtgcg gaagccgaaa tcggcgagta cctgctcgat gatgcgggcg
     1261 acgtcggggt cgtcctcgac gatggcgatc agtttgccgc tgtcctgctc cttcatgggg
     1321 tctcctcgcc cgcggcgcgt tgcagagcac gtgccagttc ctgcctgagg aagggtttgg
     1381 ccaggatggg aaagtcccgg ccgcgcgcgt cctcgtcttc ggtgtagccg ctcatcagca
     1441 cgatgcgcat cgacggacat tcccgtcgca gcagtcgcgc cagttggcgg ccgttcagtt
     1501 cgcccggcat cagcacgtcg ctgacgacga ttgcgatgtc gggaatgtgg cgcaccatgt
     1561 cgtgcgcctg gctggcgttt tccgcttcga tcaccgggtg gccgaggtcg atcagttgct
     1621 ggcggacgac gcggcggaca ttttcgtcgt cctcgaccag tagcaccagg tcgccgctgg
     1681 cttcgggcag cggcgtttcg gccacggcct gccggctggc gggcgctgcc gtctgcggca
     1741 gcacgatctc gacctgggtg ccgcgctccg gttcgctgcg gatgcgcatg ccgcccgccg
     1801 actggcggat gaagccctgc gccatggcca gccctagtcc gttgccgagg ccgaggcgct
     1861 tggtggtgaa gaagggttcg caggcatgcg ccagggtgtg cgcgtccatg ccgcaaccat
     1921 cgtcggcgac ggcgatggcg acgtagttgc ccggtgcgac gtcgaattcc gcggtttccg
     1981 gggcgtcggc cggcagttcg gtgaggtcgg cggaaatcgt cagccggccg ccttgcggca
     2041 tcgcatcgcg ggcgttgagc gcgaagttga gcagggcgct ttccagctgt gccgggtcca
     2101 ccttggcgaa caggccccct tcggcgttgc ggcaatcgac gacgatgttg tccggcagcg
     2161 agcggcggat caggcgcgcc aggtcgccga tcaactgacc gatatcgacc gcctgcggtt
     2221 ccagcggctg ttgccgtgag taagtgagca ggcggcggat caactgcacg ccgcggcgcg
     2281 ccgactgcag cgccgggctg acgaagtcgg cgattcccgg atcgtccggc cggtgatcct
     2341 ggagcgccgc cagattgccg atgatcaccg tcagcaggtt gttgaagtcg tgcgccagac
     2401 cgccggtcag gcggccgatc gcttccatct tctgcgcttg cagcaacgcg ctctgcatcc
     2461 gcttctgctc ggtgatgtcg tgcgagaaga cgaagaagcc gtgggtgtcg ccatcgctac
     2521 cgatttccgg aaccagcgtg ctgtgcgcga agaccgtctg gccgtggcgc agcatccggt
     2581 actcgtaact gatctgttcg ccggacagtg ctcgccgcac atgctcgccg acctggacat
     2641 agacttcggg accgaccacc tcgagcaccg agcggccgat cacgctgccc gccggcaggc
     2701 agtaccagtc ggcgtaaccc ttgttggcgt actggtagat ctgctgacgg tcgacgtaac
     2761 cgatgaggat ggggatggtg tcgttgatca gcctcaggcg ggcttcgctg cggccgagcg
     2821 cggcggcgat ccgctccttc tcggcgttgg cctggcgcag gttggcgttg gcgttttcca
     2881 gctgggcggt gcggcggcgg acgcgttcgt cgagctgcag gttctggtgc tcgctcaact
     2941 gctccaggta gcgctgttcc gtgatgtcgc tgtacagcgt gacgaagccg ccgtgcggca
     3001 gcggttcgcc gcgcagcagc agcaggcggc cgttggggcg gcgccgttcg ctgacgtggg
     3061 caacgaagtg gcgggcctcg gcgacgcgct cggcgacctg ggcctcgggg tcgccgggac
     3121 cgtattcgcc gcgctgcgcg ttgtagcgga tgaagtcggc gaacggcgta ccgatccgcc
     3181 ccagttcgtt cgggaaaccg agcagttcga ggaaggggcg gttccaggcg accagccgca
     3241 ggtcgcggtc gaagacactg atcccctggt cgagcaggtc gagcccgtcc tgcagcgtgg
     3301 cgagccggtt cgactggtcg gcggggaggg aggcatgagg catcgcggga ttctagagca
     3361 aggcgcctgg cttgaacgac aatgcaatga ttcgttacat tgcgccaata gttgtgcaag
     3421 agtgttctgt aatgctacgg cccacaagaa ggataccggc cgttcaagga gaacagcact
     3481 ctggcaggta ttaagaagta aaaagcgagg agattggccg ggccggcagg tccggccatc
     3541 cttatcagaa tcgtcatcgt catgcccgcc gcggcgggct gcctggcgtt cgcgtcgcgg
     3601 gcgccatctt gtctgaaagg aagctgtgaa tgagtactta caccgcaccg ctgcgtgaca
     3661 tgcagttcgt tctcaaggat atcgccggtc tcgacgagat ctgcgccttg ccgggcaacg
     3721 aggaatgttc cgttgacctg gtcgaatcga ttcttgaaga agcggccaag ttcgccagcg
     3781 gcgtgctcga tccgatcaac cgcaacggcg acaatcaggg ggccgtggtc aaggacggcg
     3841 tggtcaccac ctcgcccggt ttcagggacg cctatcagct gttctgcgag accggttgga
     3901 acgccatgcc cttctcgccg gaatacggcg gccagggcct gccggcggca gtgacgatgg
     3961 cggtcaacga aatgtggaag tcggccaaca tggcctttgc gctgtgcccg atgcttaccg
     4021 gcggcgccat cgaggcgatc gcccaccatg cttccgacga actcaagcag aaatatctgc
     4081 cgaagatggt cgaaggcgtg tggaccggca ccatgaacct gaccgagccg aacgccggtt
     4141 ccgacctggc ggcgatcagc accaaggcaa ggaagctcga cgacggcacc tacctggtca
     4201 ccggcaccaa gatcttcatc acctggggcg aaaacgactg cgccgaaaac atcatccacc
     4261 tggtcctcgc ccgcctgccg gacgcgccgc cgggactgaa gggcatctcg ctgtttctgg
     4321 tgccgaagtt cctggtcaat gacgacggct cgctcggtgc ccgcaacgat ctgatctgcg
     4381 cctcgctcga acacaagctc ggcatccacg gcagcccgac ggcggtgatg tcctacggcg
     4441 accaggaagg cgccgtcggt tacctgatcg gcgaagagaa caagggcatc ggctacatgt
     4501 tcacgatgat gaaccacgcc cgcgtcaacg tcggcctgga aggcgtcggc atcgccgagc
     4561 gcgcctacca gcacgcgctg tggtacgcca aggaacgcat ccagggcgcc atcgtcggcg
     4621 acaagtcggg cgagaagaag ccgatcctgc atcaccccga tgtccgccgc ctgctgatgg
     4681 acgtcaagtc gcgcaccgaa gcgatgcgca ccctggccta ttacgccgcc gcccagatcg
     4741 acaaggcgca cgccggcgac tccgccgcgc aggcgcgcat cgacctgctg acgccggtgg
     4801 tcaagggctg gagcaccgaa cagggcgtcg aactgtcgtc gaacgcgctg caggttttcg
     4861 gtggcgtcgg cttcatcgag gaaaccggtg ccgcgcagta ctaccgcgat tcccggatca
     4921 ccacgatcta cgaagggacg acggcgatcc aggcgaacga cctggtcggc cgcaagctgg
     4981 cgcgcgaaaa ggtgccgggt gccggcatga aggcgatgct cgccgagatc aacgccaccg
     5041 ccgacgaact cgccggtaat gccggtctcg ccaacatcgc cgccaacctg aagaacggtg
     5101 ccgcggcgct gactgccgcc gccgactgga ttctcgccaa ctacaacgac aagccgcagg
     5161 ccgtgcatgc cggttcggtg cccttcctca agctgaccgg catcgtcgcc ggtggctggc
     5221 tgatggccaa gtcggccggc gttgccgcag cccgcatcgc gaccggcgac agcgacccgt
     5281 tctaccgggc caagctggcg accgccgaat atttcgcgac gcatcaactg cccttcgcgg
     5341 ccgcttacgc ggcggaagtg atgggtggtg ccgaggccgt cttcggtctc gccgaagacc
     5401 tgttctgagc gccatcatgc gtaccgatcg cgatgcgatg gagtacgacg tcgtgatcgt
     5461 cggcggcggc cctgcggggc tgaccgcggc gatccgcgtc aagcagcagg cggcggccgc
     5521 cggtcgtgag ctgtcggtct gcctgctcga gaagggctcg gaaatcggcg cccacatcct
     5581 gtccggcgcc gtcatcgaaa cccgcgccct ggccgagttg ttccccgact ggcaggaacg
     5641 cggcgcgccg ctcaacacgc cggccggcga ggaccgtttc ctgttcctca ccgaaggtgg
     5701 ttcgttcaag ctgccgacgc cgccgcagat ggccaaccac ggcaactaca tcgtcagcct
     5761 gggcaacttc tgccgctggc tgggtgagca ggcggaagcg ctcggcgtcg agatctaccc
     5821 tggcttcgcc gccgccgaag tgctctacca cgaggatggc tcggtcaagg gcgtcgccac
     5881 cggcgacctc ggcaaccaca aggacggcca gcccggccac aacttccagc cgggcatgga
     5941 actgcacgca aggcagacga tcttcgccga aggctgccgc ggttcgctga cccgcgaact
     6001 gttcgagcgc ttcaatctgc gcgacggcgc cgatccgcag acctacggca tcggcatcaa
     6061 ggaactgtgg gaaatcgatc cggcgaaaca ccagccgggg ctgatcgtgc actcggtcgg
     6121 ctggccgttg gccaacgaca cctacggcgg ttccttcctc taccacctgg aaaacaacct
     6181 ggtcgccgtc ggtttcgtcg tcggtctcga ctacaagaat ccctggctgt cgccgttcga
     6241 ggaattccag cgctacaaga cccacccggc gatccgcggc tttttcgaag gcggccggcg
     6301 catttcctac ggcgcccgcg cgctgtcgga aggcggcttc cagtcgctgc cccggctgac
     6361 ttttccgggc ggtttgctgg tcggcgatac cgccggcttc ctcaacgtgc cgaagatcaa
     6421 gggcacgcac atggcgatgc agtcggcgat gcttgccgcc gatgccgtcg tcgcgctgct
     6481 ggccgacgcc gaaggtggcc gggaagccca ggcctacccg ctggcactgc gcgacaactg
     6541 gctgtgggac gaactgcacc gggtgcgcaa cgtgcgcccg gcgttcaagt gggggctgtg
     6601 gggcggactc gcctacggtg cgctcgacac ctttctgttc cgcggcaagg cgccgtggac
     6661 gctggcgcac cacgacgacc attcgcaatt gggcgagaag tcggcctacg ccaagatcga
     6721 ctatccgaag ccggacggca agctgacctt cgaccgtctg tcgtcggtct tcctgtcggc
     6781 gaccaaccac gaggaaaatc agcccagcca cctgcggctg aaggacgcga cggtgccggt
     6841 gtcgatcaat tacgcgaagt acggtgcgcc ggaaacgcgt tactgcccgg ccggcgtcta
     6901 tgaaatcctc ggcgaggacg aaggcgcgcc acgcttgcag atcaacgccc agaactgtct
     6961 gcactgcaag acctgcgaca tcaaggaccc gacccagaac atcgtgtgga cggtaccgga
     7021 aggcggcggc gggccgaact atccgaacat gtgaccagag caactctgcg ttggagattt
     7081 gagggagaac agcaaacgac ctcggtcttc atacttttcc ggggccgtaa ggccccgttt
     7141 tttttaatct tttttacaaa ataagctcaa atcatggtgg gcaggagtta acatgtccag
     7201 cctcttccgc aatggcttta ccgactgttc tacccatgcg catgttgaat aacagcaatc
     7261 cgctgagggt tcccggtttg ctcggcaaac tgggggtgat gacctcgatc cgcgataccg
     7321 agttgctcga gcaaagtctg ctgcgcacgc tggggccctt gctcggtgtc ctcgatacct
     7381 ctctctaccg gctcgacgaa gcgcagttgc tggtccgcgt catccattac caccgttcca
     7441 aggtcgtcga tggcgatggt gttgcccgca tgctcgaacg gatcgaggaa atcgtccagc
     7501 tttccgacgt ggcaccggaa gtcctcgccc tgaccgacaa tgtccgcctg ctcggcaagc
     7561 cctgcacccg ggtgctcgaa ggcggcatgc tgatcgccta ccccctgctc ggtgccggcg
     7621 aggtctgcgg ctatttcgtc ttctgccgcg atcacgaggt ctcggccaac gaggacgcga
     7681 cgatccgcgg cgtcctcgaa gtcttctcca actactacgc gctgctcgac gtcagccagc
     7741 gcgaccgcct gaccgggctg ttcaaccgcc aggcactgga aaacaatttc gaacgcatct
     7801 ggtcggcgat ggcgcagccc gagactttcc acgaacgcca ggacggccgg cgccgccatg
     7861 gccagggcaa gtactggctg gcggtgatcg acatcgatca tttcaagcgg atcaacgata
     7921 ccttcggcca catggtcggc gacgagatcc tgcttctcgt ctcgcgcctg atgcagaaca
     7981 ccttccgttc ctcggacctg ctctaccgct acggcggcga ggagttcgtc gccatcgtcg
     8041 ccgccgagaa cgaggcgatc gcccgcaacg tcttcgagcg cgtgcggttg gcgatcgagg
     8101 cgcacagctt tccgcgcatc gaccgtctgt cggtcagcat cggctacgcc gaagtcgatc
     8161 cggaaatcct gccggtggaa gttctcagcc gggccgaccg cagcctctac caggccaagc
     8221 aggacggacg caaccgcgtc tacgatttcc aggaactggt cgccagcgga atttttccca
     8281 gccccagcta cggcgacgcc gaactgttct aagccggtgc cgccgccttg atcggcagcc
     8341 agatacggaa gcggctgccc ttgcccggct cgctgtcgac gtcgatgtag ccgcggtgct
     8401 tcttgacgat gatgtcgtag gaaatcgaca gtccgagacc ggttcccttg ccgaccggct
     8461 tggtcgtgaa gaagggctcg aagatgcgct ggcgtaccgc gtcgctcatg ccgcgcccgg
     8521 tatcctcgac ctcgaaccac acccgttcgc cggcgacacc ggagcggacg gtgatcctgc
     8581 catgttcctg aatcgcctgt gcggcgttga ccagcaggtt catgaagacc tggttgatct
     8641 gcgccgggac gcaatcgact tccgggattt cgcccagttc gagaatgacg tcggccttgt
     8701 acttcagctc gttgcgcacg acgttgagcg tgctctgcat cgccgcgttg aggtcggcat
     8761 gctggcgttc ggtctggtcg acgcgggaga agtccttgag atcctggacg atgcgcgtca
     8821 cccggccgag gccctcctgc gattcggcga gcagcgcggg caggtcctcg cgcaggaaat
     8881 cgaggtcggc ggcacgccgc gccgcgccga tggcctcggc gttgccacct tcgtaggcgg
     8941 cgatgacgga gagtagctgt tcgacgtaat gtttgagcga actcaggttg gaattgacga
     9001 agccgatcgg gttgttgatt tcgtgggcga cgccggcggc caactggccg atcgccgcca
     9061 ttttctcgga ctgcagcagt tgctgctgcg cttcctcgac cttgtgcagc agcgcggtca
     9121 gctcggcttg ctcgcgttcc agttgccggt tggcctgttc gagggcgccg gtcctttcgg
     9181 cgaccagggc ttccagttgt tcctggtg
//
LOCUS       DAOSDX010000007         8976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2880_length_8976_cov_3.465082, whole genome shotgun sequence.
ACCESSION   DAOSDX010000007 DAOSDX010000000
VERSION     DAOSDX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8976
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_8976_cov_3.465082"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLN02_00305"
     CDS             complement(<1..920)
                     /locus_tag="PLN02_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI50792.1"
                     /translation="MNAPTPKEIMEFTKTPGWLDWYAGPSQPAFKLPAGAVDAHCHVF
                     GPGAEFPYAPERKYTPCDASKQQLYALRDHLGFARNVVVQATCHGADNRAMLDALVHS
                     GGKARGVATVRRSVSDEELQAMHDAGVRGVRFNFVKRLVDFTPKDELLEIAGRIARFG
                     WHVVIYFEAVDLPELWDFFTALPTTVVVDHMGRPDVGKPVDGPEFQLFLKFMRDNPNV
                     WSKVSCPERLSLTGPKAVAGERNAYADVVPFARRVVEEFPDRVLWGTDWPHPNLKDHM
                     PDDGLLVDFIPHIAPTAELQQKLLVANPMRL"
     gene            complement(923..1618)
                     /gene="ligK"
                     /locus_tag="PLN02_00310"
     CDS             complement(923..1618)
                     /gene="ligK"
                     /locus_tag="PLN02_00310"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HOI50793.1"
                     /translation="MTQPMNQLGIVKRNIQRAERAAVEKLSRFGVATIHEAMGRVGLM
                     KPTIRPIYAGAHLCGTAVTVLLQPGDNWMLHVAAEQLQPGDVCVAGCTADSTDGFFGD
                     LLATSYQSRGAVGLIIDGGVRDVKDLTAMGFPVFSRAIHAKGCTRATLGSVNIPVVCA
                     GAIVNPGDVVIADDDGVVVVPAALAQQVADAAEAREHNETAKRARFAAGELGLDMYAL
                     REPLAKAGLKYID"
     gene            complement(1629..2654)
                     /locus_tag="PLN02_00315"
     CDS             complement(1629..2654)
                     /locus_tag="PLN02_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI50794.1"
                     /translation="MIIDIHGHYTTAPKALEEWRNRQVASLKDPALAPKLSELQISDD
                     DLRQTIEQNQLKLMKERGADLTIFSPRASFMAHHIGDYQTSATWAAICNELCFRVAEL
                     FPDNFIGAAMLPQSPGVDPQTCIAELEKCVKEYGFVGINLNPDPSGGHWTSPPLTDRH
                     WYPIYEKMVEYDIPAMIHVSTSCNACFHTTGAHYINADTTAVMQLIQGDLFKDFPTLK
                     FVIPHGGGAAPYHWGRYRGLAQELKKPLLDDHLLNNVFFDTCVYHQPGVDLLTRVIPV
                     KNVLFASEMIGAVRGIDPQTGHYYDDTKRYIEAADLTAEERYMIYEGNARRVYPRLDA
                     ALKARGR"
     gene            complement(2878..4020)
                     /locus_tag="PLN02_00320"
     CDS             complement(2878..4020)
                     /locus_tag="PLN02_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOI50795.1"
                     /translation="MKKKIIALAVPAALCAPVIAQENVQVYGIFDIGVASIDAGGKER
                     RNKVDSGLLRTSRLGFKGSEDLGNGLKVNFVLEYRLNVDLNEGIGGNYRSDAAGVQGS
                     TSGPARQQLLGLSGNFGTVAFGRMNTTAFDWAVKYTVLGASAFDVTGNNAMAAGSRIN
                     PVGDIRLSNVIAYKSPAFAGFRFDVNYARPIEEAAAGNGRLETTQLGLYYDNGPLSVG
                     AVFDNIDGHGNATLTLSSLAFARGAGFPPGTTVVRYDDLEVKSQNLGVAYDFGVLKAK
                     ATYQRDKFMDLSANKVWGVGVEVPVSAKGTVHAVYSASSVKTQNKADSQGFSLAYTHN
                     LSKRTIAYAGLSWQDNERNGTSGIWATAPDAGKDVKMLAAGITHMF"
     gene            complement(4187..5581)
                     /locus_tag="PLN02_00325"
     CDS             complement(4187..5581)
                     /locus_tag="PLN02_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI50796.1"
                     /translation="MTTQNRPLDVQKFIDDNKFSRFQWLVFAVSFLVILLDGFDTAAI
                     GYIAPSLLKEWGIAKPQLAPVLSAALAGLAVGAVLIGPFADRFGRKYLLTGAVALFGV
                     ASLASTTSGSLDELVIWRFVTGLGLGAAMPLAVTLASEFAPTRRRAVLTSAMFTGFPL
                     GAALGGFLAAWMIPHWGWRSVLELGGVLPIVLALFMLFTLPESVRYLVARGAPAERIR
                     AVLARISASIGKAERFVLGETVVAKVANDQGKSGVALILSRSYVVGSAMLWLTYGMGI
                     VIFYALINWMPILLKDAGLEPQTALLVTSLFPLGGVGAIAFGLLMDKFNANRVIMIGY
                     LITAACVYAIGQVAGNLGWLVVMVFVGGAVMNTTQTSMPALAAAFYPTAGRATGVAWM
                     MAIGRIGAVAAPFLVAELQRRHFDFAQIFAVLAIPGVIAAIALFVKNASHPEKSVADA
                     EPDEAPAVPVTSQS"
     gene            complement(5629..6717)
                     /locus_tag="PLN02_00330"
     CDS             complement(5629..6717)
                     /locus_tag="PLN02_00330"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HOI50797.1"
                     /translation="MQTPIKTLFMRGGTSRGPFFREADLPADIASRDRVLLAVMGSPD
                     KRQIDGLGGANPLTSKVGIVRPSQTAGVDLDFLFAQLQPEKDSVDTTPNCGNMLAAVV
                     PFALETGMLAAQGETTTLRVLTLNTGMQCDITVETPGGRVNYAGAAHIDGVPGSSAPI
                     TINFLDTAGSVCSGLLPTGRARDTIDGIEVTCIDNGMPLVILRAADVGCSGYESVAEL
                     NADAPLKARIERLRLACGEAMGLGDVSAKNYPKMTLVAPPQAGGSITTRSFIPHVCHD
                     AIGVLAAVTVGTACVLDGSVTAGIAVVPAGHSKKISVEHPTGEFSVELELDPENPQNV
                     RRAALLRTARLIMRGEVMVPAEVWPGKA"
     gene            complement(7161..8093)
                     /locus_tag="PLN02_00335"
     CDS             complement(7161..8093)
                     /locus_tag="PLN02_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOI50798.1"
                     /translation="MSILVIAEHDNAQLKASTLNTVAAASRIGGEIHVLVAGADCAAV
                     ARQAASLQGVAKVRLADAAHYQSQTAENLSALVLAQAAGYSHLLAPATTFGKNLLPRV
                     AALLDVAQISEIVGVDSADTFVRPIYAGNALATVRSADPVKVVTVRTTAFDPVASAGN
                     AAIENVANANDTAQCALLGRELSKSERPELAAASTIVAGGRGLGSGESYRALLEPLAD
                     KLGAALGASRAAVDAGFVPNDYQVGQTGKIVAPDLYLAIGISGAIQHLAGMKDSKVIV
                     AINKDPDAPIFQVADYGLVGDLFEAVPQLVAAIR"
     gene            complement(8096..8845)
                     /locus_tag="PLN02_00340"
     CDS             complement(8096..8845)
                     /locus_tag="PLN02_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOI50799.1"
                     /translation="MKILVPVKRVVDYNVKIRVKADGSGVDLANVKMSMNPFDEIAVE
                     AAVRLKEAGIASEVVAVSCGVAACQETLRTALAIGADRAILVDSDAELQPLAVAKLLK
                     VLCDREQPQLVICGKQAIDDDANQTGQMLAALAGWPQATFASKIAIADGQATVTREID
                     GGLETLQLALPAVITTDLRLNEPRYATLPNIMKAKKKPLATQTPAELGVDVTPRLRTL
                     KVAEPPSRSAGVKVADVAELLAKLKNEAKVI"
BASE COUNT         1514 a         2803 c         3080 g         1579 t
ORIGIN      
        1 aggcgcatcg ggttggccac gagcagcttt tgctgcaact cggcggtcgg cgcgatgtgc
       61 gggatgaaat cgaccagcaa gccgtcgtcc ggcatgtggt ccttgagatt cgggtgcggc
      121 cagtcggttc cccatagcac gcggtcggga aactcctcga cgacgcggcg ggcgaagggc
      181 acgacgtcgg cgtaggcatt gcgttcgcca gcgacagcct tgggcccagt cagcgacagg
      241 cgctccggac aactgacctt gctccagacg ttcggattgt cgcgcatgaa cttcaggaac
      301 agttggaatt ccgggccatc gaccggcttg ccgacgtcgg ggcggcccat gtggtcgacg
      361 acgacggtgg tcggcaaggc ggtgaagaaa tcccacagtt ccggcagatc gaccgcctcg
      421 aagtagatca cgacgtgcca gccgaacctg gcgatgcggc cggcgatttc cagcagttcg
      481 tccttcggcg tgaaatcgac caggcgcttg acgaagttga aacgcacgcc gcgcacgccg
      541 gcgtcgtgca tcgcctgcag ttcctcgtcg ctgacgctgc ggcgcacggt ggcgacgccg
      601 cgcgccttgc cgccgctatg gaccagcgcg tcgagcatgg cccggttgtc ggcgccgtga
      661 caggtggcct ggacgacgac gttgcgggca aagccgaggt ggtcgcgcag cgcgtaaagc
      721 tgctgtttcg aggcatcgca gggcgtgtac ttgcgttccg gggcgtaggg gaattcggcg
      781 ccggggccga agacgtggca gtgcgcgtcg accgcgccag ccggcaactt gaaagcgggc
      841 tggctcgggc cggcgtacca gtcgagccag ccgggggtct tggtgaattc catgatttcc
      901 ttgggcgtcg gtgcgttcat gttcagtcga tgtacttgag gccggccttg gccagcggtt
      961 cgcgcagcgc gtacatgtcg agccccagtt cgccggcggc gaaacgggcg cgcttggcgg
     1021 tctcgttgtg ctcgcgcgct tcggcggcgt cggccacctg ctgggccagg gcggccggca
     1081 cgacgacgac gccgtcgtcg tcggcgatca ccacgtcgcc gggattgacg atggcaccgg
     1141 cgcagaccac cgggatgttg accgagccga gcgtcgcccg cgtgcagccc ttggcgtgga
     1201 tcgcccgact gaacaccggg aagcccatcg ccgtcaggtc cttcacgtcg cgcacgccgc
     1261 cgtcgatgat caggccgacg gcaccgcgcg actggtagga agtggcgagc aggtcgccga
     1321 agaagccgtc ggtgctgtcg gcggtgcagc cggcgacgca gacgtcgccc ggctgcagtt
     1381 gttcggcggc gacgtgcagc atccagttgt cgccgggctg cagcaggaca gtcaccgccg
     1441 tgccgcacag gtgggcgccg gcgtagatcg ggcggatcgt cggcttcatc aggccgaccc
     1501 gtcccatcgc ctcgtggatg gtcgcgacac cgaaacgcga cagtttctcg acagcggcgc
     1561 gctcggcacg ctggatattg cgcttgacga tgccaagctg gttcatcggt tgggtcatgg
     1621 tgttctcctc agcggccacg ggccttcagg gcggcatcga gccgcggata gacgcggcgg
     1681 gcgttgcctt cgtagatcat gtagcgctct tcggcggtca ggtcggcggc ctcgatgtag
     1741 cgcttggtgt cgtcgtagta gtggccggtc tgcggatcga tgccgcgcac agcgccgatc
     1801 atctcggaag cgaacaacac gttcttgacc gggatgacgc gggtcagcag gtcgacgccc
     1861 ggctggtgat agacgcaggt gtcgaagaag acgttgttga gcaggtggtc gtcgagcagc
     1921 ggcttcttca gttcctgcgc caggccgcgg taacgccccc agtggtaggg cgcggcgccg
     1981 ccgccgtgcg ggatgacgaa cttcagggtc gggaagtcct tgaacaggtc gccctggatc
     2041 aactgcatca ccgccgtcgt gtcggcgttg atgtagtgcg cgccggtggt gtgaaagcag
     2101 gcgttgcaac tggtcgatac gtggatcatc gccgggatgt cgtactcgac catcttttcg
     2161 tagatcgggt accagtggcg gtcggtcagc ggcgggctgg tccagtggcc gcccgacgga
     2221 tcgggattga ggttgatgcc gacgaagccg tattccttga cgcacttttc cagttcggcg
     2281 atgcaggtct gcggatcgac acccggcgac tgcggcagca tggcggcgcc gatgaagttg
     2341 tccgggaaca gctcggcaac gcggaagcac agctcgttgc agatcgccgc ccaggtcgcc
     2401 gaggtctggt agtcgccgat gtggtgggcc atgaagctgg cgcgcgggct gaagatggtg
     2461 aggtcggcgc cgcgttcctt catcagcttg agctggttct gttcgatggt ctggcgcagg
     2521 tcgtcgtcgc tgatctgcag ttcgctgagc ttgggcgcca gcgccgggtc cttgaggctg
     2581 gcgacctggc gattgcgcca ttcctcgagc gctttcggcg ccgtcgtgta gtggccgtgg
     2641 atgtcgatga tcatgccgtt ttccttttcg ttcgttgtcg tccgtcgtgc gccggctgcc
     2701 cggcgccgca gtgatttcag gaaaggattc tgcgcaagcc cccgctcact gaacaggaaa
     2761 ggccccgact agctgctatg ccttttcgtt atgaaagagg cgaaatttgc aaaaaacccg
     2821 tgttccgcca gtttgcagtg acggaacacg ggccgcaggc gatgccggaa gcggcgatca
     2881 gaacatgtgc gtgatgccgg cggcgagcat cttgacgtcc ttgccagcat ccggcgcggt
     2941 cgcccagatc ccggaagtgc cgttgcgctc gttgtcttgc caggagaggc cggcataggc
     3001 gatggtgcgc ttgctcaggt tgtgcgtgta ggccagcgag aagccctgcg aatccgcctt
     3061 gttctgggtc ttgaccgagg aagcggaata gacggcatgc acggtgccct tggcggatac
     3121 cggcacttcc accccgacgc cccagacctt gttggccgag agatccatga acttgtcgcg
     3181 ctgatacgtc gccttcgcct tcaggacgcc gaaatcgtag gcgacgccaa ggttctggct
     3241 cttcacttcg agatcgtcgt agcgcaccac cgtcgtcccc ggcgggaagc cggcaccgcg
     3301 cgcgaaggcc agcgaactca gggtcagcgt ggcgttgcca tgcccatcga tattgtcgaa
     3361 gacggcgccg accgacagcg gaccgttgtc atagtacagc ccgagctggg tggtctcgag
     3421 gcgaccattg ccggcggcgg cctcctcgat cgggcgggca tagttcacgt cgaaacggaa
     3481 gccggcaaac gctggcgact tgtaggcgat gacgttggag aggcgaatat cgccgaccgg
     3541 attgatccgg ctgccggcag ccatcgcgtt gttgccggtg acgtcgaagg cggaggcccc
     3601 gagcaccgtg tatttgaccg cccagtcaaa ggccgtcgtg ttcatgcggc cgaaagcgac
     3661 ggtaccgaag ttgccgctca ggccgagcag ttgctggcgg gccgggccgg aagtgctgcc
     3721 ctggacgccg gccgcatcgc tgcggtagtt gccgccgatc ccttcattga gatcgacgtt
     3781 gagccggtac tcgagcacga agttgacctt cagcccgttg ccgagatcct cgctaccctt
     3841 gaagccgaga cgcgaggtgc gcagcaggcc gctatcgacc ttgttgcgcc gctccttgcc
     3901 gccggcatcg atgctggcga caccgatatc gaagatgccg tagacctgga cattctcctg
     3961 cgcgataacc ggtgcgcaaa gcgcggcggg cactgccagg gcgatgattt tctttttcaa
     4021 ttgagtactc ctcctcaaac aaattgacct tccgcagcac ggaaagcatc ggcgaatgac
     4081 attcgccctc ctcctcagct tgccggacgg attccggcaa gctgcacggc gccccgccgc
     4141 gactgcccag gcagcccgca gcgagagtcg caatgttcct tgtcgttcag gactggctgg
     4201 tgaccgggac cgccggcgcc tcgtccggct ccgcgtcggc gacggacttt tccgggtgcg
     4261 aggcgttctt gacgaacagg gcgatcgcgg cgatcacgcc gggaatcgcc agcaccgcga
     4321 aaatctgcgc gaaatcgaag tggcgacgtt gcagttcggc gaccaggaag ggcgcggcga
     4381 cggcgccgat gcggccgatc gccatcatcc aggcgacccc ggtggcccgc ccggcggtcg
     4441 ggtagaaagc cgcggccagc gccggcatgc tggtctgcgt ggtgttcatc acggcaccgc
     4501 cgacgaagac catgaccacc agccaaccga ggttgccggc gacctggccg atcgcataga
     4561 cgcaggcggc ggtgatcagg taaccgatca tgatcacgcg gttggcgttg aacttgtcca
     4621 tcagcaggcc gaaggcgatg gcgccgaccc cgcccagcgg gaacagcgag gtgacgagca
     4681 gcgccgtctg cggttcgagg ccggcatcct tcagcaggat cggcatccag ttgatcagcg
     4741 cgtagaaaat cacgatgccc atgccgtagg tcagccacag catcgccgaa ccgacgacat
     4801 aggagcgcga caggatcagc gcgacgccgg acttgccctg atcgttcgcc accttggcga
     4861 caacggtctc gccgaggacg aaacgttcgg ccttgccgat gctcgccgaa atgcgcgcca
     4921 gcacggcgcg gatacgctcg gcgggagcgc cgcgggcaac caggtagcgc accgattccg
     4981 gcagcgtgaa caacatgaac agggcgagca cgatcggcag cacgccgccg agttcgagca
     5041 cgctacgcca gccccagtgc gggatcatcc aggcggcgag aaaaccgccc agcgcggcgc
     5101 cgagcgggaa accggtgaac atcgcactgg tcaggacggc acgccgacgg gtcggcgcga
     5161 actcgctggc cagtgtcacc gccagcggca tcgcggcgcc caggccgagg ccggtgacga
     5221 aacgccagat gaccagttcg tcgagcgagc cggaggtcgt cgacgccagg ctggcgacgc
     5281 cgaacagggc aaccgcgccg gtcagcaggt acttgcggcc gaaacggtcg gcaaagggac
     5341 cgatcagcac ggcgccaacc gccaggccgg cgagcgcggc actcagcacc ggcgccagtt
     5401 gcggcttggc gatcccccac tccttcaaca gcgacggggc gatgtagccg atggcggcgg
     5461 tgtcgaaacc atcgagcagg atgaccagga agctgacggc gaagaccagc cactggaagc
     5521 gggaaaactt gttgtcgtcg atgaacttct gcacatcgag cggtcgattc tgagttgtca
     5581 tgaaagtctc ctccgccttg gctggcggtc tgttatttag aaatactgct atgccttccc
     5641 tggccagacc tccgctggca ccatcacctc gccgcgcatg atcagccgcg ccgtgcgcag
     5701 cagcgcggca cggcggacgt tctgcgggtt ttccggatcg agctcgagct cgacgctgaa
     5761 ttcgccggtc ggatgctcga ccgagatctt cttgctgtgg ccggccggga cgacggcgat
     5821 gccggcggtc accgaaccgt cgaggacgca ggcagtgcca accgtcaccg ccgccaggac
     5881 gccgatcgcg tcgtggcaga catgcgggat gaagctgcgc gtcgtgatgc tgccgcccgc
     5941 ctgcggcggc gcgaccaagg tcatcttcgg gtaatttttg gcgctgacgt cgcccagccc
     6001 catcgcctcg ccgcaggcca gacgcaggcg ctcgatgcgc gccttcagcg gcgcatcggc
     6061 gttgagttcg gcgacgctct cgtaaccgct gcacccgacg tcggcagcgc gcaggatgac
     6121 gagcggcatg ccgttgtcga tgcaggtgac ctcgatgccg tcgatcgtgt cgcgcgccct
     6181 gccggtcggc agcaggccgg agcagaccga accggcggta tcgaggaaat tgatcgtgat
     6241 cggcgccgaa ctgcccggca ccccgtcgat gtgcgcggcg ccggcgtaat tgacgcggcc
     6301 gcccggggtt tcgacggtga tgtcgcactg catgccggta ttgagcgtca gcacgcgcag
     6361 cgtcgtcgtt tccccctgtg cggcgagcat gccggtttcc aacgcgaagg gcacgaccgc
     6421 ggccagcatg ttgccgcagt tgggcgtcgt atcgacgctg tccttctccg gctgcaactg
     6481 ggcgaacagg aaatcgagat cgacgccggc ggtctggctg ggacgaacga tgccgacctt
     6541 gctggtcagc ggattggcgc cgcccagacc gtcgatctgg cgcttgtcgg gcgagcccat
     6601 gaccgccagc agcacgcggt cgcggctggc gatgtcggca ggcagatcgg cttcccggaa
     6661 gaaggggccg cgcgaggtgc cgccgcgcat aaacagggtt ttgatcggag tttgcatgga
     6721 aatcattgtg tacttcgctt ttccggcgga acaggaaagg gaacgactag cagctatacg
     6781 ctttcgttat gggggcggtc ccccaaagcg ccgcccctgg cctcgcccag cccttccggg
     6841 attccatgca atccttcaat ttcctctcct ggcctgggtt ttcgggcgcc accccggttt
     6901 cacccggggc tccgcaatga caagtaattg agtcagttcg ttatcaggaa ccctgacaca
     6961 aaggcgatgt tacgccatta ctcgtaaaac acaatggtga aatgctccac taatcagtaa
     7021 tttttagcct cgcatccggg gcggcggacg ccgacctgtc gccccgcgcg gcagacgaaa
     7081 aaaagccggc tcggcgatga gacgccgagc cggcaagctg ggaccggaaa agcgtcccgg
     7141 tttccagagg agcagagaaa tcagcgtatg gcagcgacca gttgcggcac cgcttcgaag
     7201 agatcgccga ccaggccgta atcggcgacc tggaagatcg gcgcgtcggg gtctttgttg
     7261 atggcgacga tcactttcga atccttcatc ccggccaggt gctggatggc gccggaaatg
     7321 ccgatcgcga gatagagatc gggcgcgacg atcttgccgg tctggccaac ctgatagtcg
     7381 ttcggtacga agccggcatc gacggcggcg cgggaagcgc cgagcgcggc gccgagcttg
     7441 tcggccagcg gctccaggag ggctcggtag ctctcgccac tacccaggcc gcgaccgccg
     7501 gcgacgatgg tgctggccgc agcgagttcg gggcgttcgg acttgctcag ttcgcggccg
     7561 agcagcgcgc actgcgccgt gtcgttggcg ttggcgacgt tctcgatggc cgcgttacca
     7621 gcgctggcga ccgggtcgaa ggcggtcgtg cgcaccgtga cgaccttgac cggatcggcg
     7681 ctcctgaccg tggccagggc attgccggcg tagatcgggc gcacgaaggt atcggccgag
     7741 tcgacgccga cgatctcgga aatctgcgcc acgtcgagca aggcggcgac gcgcggcagc
     7801 aggttcttgc cgaaggtggt ggcgggcgcc aggaggtggc tgtagccggc agcctgggcg
     7861 agcaccaggg cgctcaggtt ttccgccgtc tggctttggt aatgcgcggc gtcggcgagg
     7921 cggaccttgg ccacgccctg caggctggcc gcctggcggg cgacggcagc gcaatcggcc
     7981 ccggcaacca gcacatggat ttcgccgccg atcctgctgg cggcagccac ggtattgagg
     8041 gtggacgcct tgagctgggc gttgtcgtgt tcggcaatga cgagaatgct catgatcaga
     8101 tcaccttggc ttcgttcttc agcttggcca gcagttcggc gacgtccgcc accttgaccc
     8161 cggcgctgcg cgaaggcggt tcggcgactt tcagggtgcg caggcgcggc gtcacgtcga
     8221 cgcccagttc ggcgggcgtc tgcgtggcaa gcggtttctt cttcgccttc atgatgttgg
     8281 gcaaggtcgc gtaacgcggt tcgttcaagc gcaggtcggt ggtgatcacc gccggcagcg
     8341 cgagctgcag ggtttccagg ccaccgtcga tttcccgggt gaccgtggcc tggccgtcgg
     8401 cgattgcgat cttcgaggca aaagtcgcct gcggccagcc ggcgagcgcg gccagcatct
     8461 ggccggtctg gttggcgtcg tcgtcgatcg cctgcttgcc gcagatcacc agttgcggct
     8521 gttcccggtc gcacaggact ttcaacagct tggcgacggc cagcggctgc agttcggcat
     8581 cgctatcgac caggatggcg cggtcggcgc cgatggccag tgccgtgcgc agggtttcct
     8641 ggcaggcagc aacgccgcag gagacggcga cgacctcgct ggcgatgccg gcttccttga
     8701 gccggaccgc cgcctcgacg gcgatttcgt cgaagggatt catgctcatc ttgacgttgg
     8761 ccaggtcgac gccgctgcca tccgccttga cgcggatctt gacgttgtag tcgacgaccc
     8821 gtttgaccgg gaccagtatc ttcatggctt tccttccgtg gaatggtagc gacccagacc
     8881 gagcaggcgc atcgcgttct gcttcaggat cagcggcgcg acttccggcc ggaagccggc
     8941 cttcgcgaaa tcggccagcc agcggtcggg ggtgat
//
LOCUS       DAOSDX010000008         8824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_2936_length_8824_cov_2.939332, whole genome shotgun sequence.
ACCESSION   DAOSDX010000008 DAOSDX010000000
VERSION     DAOSDX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2936_length_8824_cov_2.939332"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLN02_00345"
     CDS             <1..181
                     /locus_tag="PLN02_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOI50800.1"
                     /translation="PKNTARVFMMREFLDLDTGEVCAAAGISTSNCHVILHRARSALR
                     GCLEKGWFLAGERPC"
     gene            175..405
                     /locus_tag="PLN02_00350"
     CDS             175..405
                     /locus_tag="PLN02_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOI50801.1"
                     /translation="MLNCKDATHRMSEAEDRDLGLTERLELSLHLALCRGCRNYQQHL
                     HVLREACRGYREELTGVDGTVSGSGQQEPPAA"
     gene            630..1022
                     /locus_tag="PLN02_00355"
     CDS             630..1022
                     /locus_tag="PLN02_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOI50802.1"
                     /translation="MQNLTHWERSYSVGNWMLDNQHRNIMILCQQTIDRIAVDQPFRS
                     AGSELLIFTGEHFSAEERLLAMTGYATLEHHREEHRRCLAALEQMLDDAASGRISPEM
                     LGDALSRWCVGHILDSDRQFSPFIQRRH"
     gene            1243..3939
                     /locus_tag="PLN02_00360"
     CDS             1243..3939
                     /locus_tag="PLN02_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI50803.1"
                     /translation="MNTIRKLIAVATTLAVLLILTLVAAAWFGFDRNLDALRTEREVT
                     QPALRAMLQARFHVVQIQQFLTDVSATGDAQAYEEARRNLQQAKAHLARLAELNPSLA
                     AELGQLQKALDDFHAAGVDMAQAYVSAGREAGNAKMQAPEVGFDARAVKLTAQLDRME
                     QEISELAEQAAATSLDMGASARTQTLLLGLVVALCVVGAGRHLRRLLYRQLGGEPALA
                     VAVANRVANNELDFDIPLTADNTDSLMAALKRMQRNLLARTQAEHETAQEMLRVKIAL
                     DNVSTGVMIADPERKIIYVNKAVQAVLKNAEADIRQQLPNFSADQLIGQNIDALHKNP
                     ALQARLLGEFTRTHSAQLQIGERHMTVTANPVITADAARLGAVAEWRDRTHEVQAQGE
                     IRSILDAAVQGDFSRRIKTRDKEGFFLDMALGMNQLLGVVLEALDDVARVLSGLSRGD
                     LSQRIESDYEGTLGQLKDDTNATTEQLRSVVSQIKYAADAINVAAKEIASGNQDLSHR
                     TEEQASSLEETASSMEQLNSTVRNNAELASKAAELAANSNLAAERGGAMVGRVVDTMG
                     GIQASSKKIADIIGVINSIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRNLAQRS
                     ADAAKEIKALIDESVGKVEGGVQLVHDTGETIREVVVAFQQLARLVNGIAEASREQAS
                     GIEQVSGAVSQMDEVTQQNAALVEEAAAAAESLEEQVEGLSRSVNIFSLDGKALTLGG
                     SGGVDFDAIIQAHLQWKFKLQQYLDGKGEALDPAVVGCDDKCAMGKWIYSDGKASYGN
                     DPLFEEMRRNHAAFHCCAGDVIRLAQKGEKAEAALRLRDDFAAQSALTVGQIRAMKKR
                     HGHGKTLAPVPLAPAGKRAAPPSTVPALAPARQSGENEWIEF"
     gene            complement(3974..4963)
                     /locus_tag="PLN02_00365"
     CDS             complement(3974..4963)
                     /locus_tag="PLN02_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOI50804.1"
                     /translation="MQAARLNPLRWQRQFWLALSLSLLAFAARAFAPPAFQGDVLDQA
                     GLLSAEERATLSQRIAELRSQDGIWAAVFIAASLQGDSIENAALKTFEQWRLGRADKD
                     NGLLVMVAPNERKMRIEVGYGLEGTLTDALSKRIVIDIYQPAFRENRYPDGLLAGFEA
                     MRRSMQGDPAALAELTGGDPAEQSAQLDGAIFLQRLLVLWLGNLLFPLLYWLISRRRV
                     PKSTDLGRKTRTAFVLFAFLGFFFGVFFGVFSQVFSDAPEIAWGLLGANALFGLVFGL
                     PALFGKPGQGGGSGTTYSNWESVSSSSSWRSGDSDSSSSGGGSSGGGGASGDY"
     gene            complement(4978..6054)
                     /locus_tag="PLN02_00370"
     CDS             complement(4978..6054)
                     /locus_tag="PLN02_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50805.1"
                     /translation="MEWQQVLDTFSRMPAGQRQVWWLAAAGLLALVLALLWLESRCFK
                     RSGRLAGWLFVRLVSALAAPLVLAVLIVPARSVGGPEALAVFYVALLLVAPPLWFGSH
                     LLAGRWARPALTGGESLALAVSGLAILAIPGSAFFAAEAPLSAAAREIGRARTLPADN
                     IPLRHTVQPVQRYHLPGVGLIYSQSLLAPADTRLLAVELRQGGLWPVDQGSAHPVYCT
                     NGNDLHLMWSAKEAPPYLRLHWEKGFAGRQRSEFTPALDFANAPPAAEFTVAFRADGV
                     DPVVPIPRPRAYLILEQPGLMPYTQMLGSPPEAGEKRSSDCVLTGFQRWTPGGDWQVR
                     AIGLSFQLPGGELLRSLIERPEPG"
     gene            6221..6745
                     /locus_tag="PLN02_00375"
     CDS             6221..6745
                     /locus_tag="PLN02_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOI50806.1"
                     /translation="MPTPLPVYLAGYPPSLTEPVARLLAQGRLGEALRRKYPSAHTVR
                     TDRALHDYVQALRADHLRNAPQPSKILFDGKLQSVGRALGTHTRIARVQGGKLKTKHE
                     IRIAAIFREAPPEFLRMIVAHELAHLREGDHDKAFYALCCHIEPDYHQLEFDTRVYLC
                     HLAAGGTPLWPTAV"
     gene            6869..8371
                     /gene="nifB"
                     /locus_tag="PLN02_00380"
     CDS             6869..8371
                     /gene="nifB"
                     /locus_tag="PLN02_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HOI50807.1"
                     /translation="MELPVISNNAPAAEGGGCSSSGCGTAPDGLAHLPDHIRAKVQDH
                     PCYSEQAHHYFARMHVAVAPACNIQCNYCNRKYDCSNESRPGVVSELLTPDQAIKKVL
                     AVAAEIPQMTVLGIAGPGDPLANPGRTFDTFRQLSEKAPDIKLCVSTNGLSLPEHVDE
                     IAKHNIDHVTITINCVDPEVGAKIYPWIYWQNKRIFGIEGAKILIEQQQKGLQMLTER
                     GILVKVNSVLIPGVNDQHLKEVSKIVKEKGAFLHNVMPLIAEAEHGTYYGIMGQPEPS
                     QEMLQELQDACAGDMAMMRHCRQCRADAVGLLGEDRGDEFTMDKIDVMDIDYEAAMVR
                     RKQVHDEIIDKLEAERGVAKPKPTDIPEGSRPVLMAVAGKNGLVAEHFGHAREFLIYE
                     ASPEGVRFISHRKTELYCGGMDACGDGDLVEAGGTESLLDRNIRILAGCEVVLCSKVG
                     YEPWAKLEAAGIAPNGEHAMEPIEEAVMAVYKEIAATGKLLPQADTLKVA"
     gene            8372..8650
                     /locus_tag="PLN02_00385"
     CDS             8372..8650
                     /locus_tag="PLN02_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI50808.1"
                     /translation="MALQITESCVNCWACVDVCPNDAIYEDKPHFLIEPDKCTECLGD
                     HADPQCAAICPIEMAIVNELGEFLNPPGSLTGIPIEKLKELGLWRDAA"
BASE COUNT         1744 a         2901 c         2858 g         1321 t
ORIGIN      
        1 gccgaagaac accgcccgcg tcttcatgat gcgcgaattc ctcgacctcg ataccggcga
       61 agtctgcgcc gccgctggca tcagcaccag caactgccac gtcatcctgc accgcgcccg
      121 cagcgcgctg cgcggctgcc tggaaaaagg ctggttcctc gccggagaac gcccatgctg
      181 aactgcaagg acgccacgca ccgcatgtcg gaagccgaag accgcgacct cggcctcacc
      241 gaacgcctcg aactcagcct ccacctcgcc ctgtgccggg gctgccgcaa ctaccagcag
      301 catctgcacg tgctgcgcga ggcgtgccgg gggtatcggg aggaactgac gggagtggac
      361 ggtaccgttt ccggcagcgg acaacaggaa ccaccggctg cttgatgctg cattgtcact
      421 tcgtacaccg cacaagtgtt acgaccggct gaatcgccaa tctgcaagct tcaggaacaa
      481 gcccgggact cgcaagtcca aacaagcgtc aggaggtcgc cgggagattc atgcgcacag
      541 aataccccag acgcccgacc ggattagctg gcacagcggt ttcccctgac cggatgccag
      601 cgtcggcagc aaagacagga gacatcgcaa tgcagaatct gacccactgg gaacgaagct
      661 acagcgttgg taactggatg ctcgacaacc agcatcgcaa catcatgatc ctgtgtcagc
      721 agacgataga ccggatcgcc gtcgaccagc ccttccggtc ggctggcagc gaattgctga
      781 tcttcaccgg cgagcatttt tccgccgagg aaaggctgct tgcgatgacc ggctacgcca
      841 cgctcgagca tcaccgggaa gaacatcggc gctgcctcgc cgcgttggaa caaatgctcg
      901 atgatgccgc cagcgggcgg atttcacccg aaatgctggg cgatgccctg tcacgctggt
      961 gcgtgggcca tatcctcgat tccgaccgcc agttctcgcc tttcatccag cgccggcact
     1021 gaagccggga ggcttgcccg atgaaacaag gcggcattgc taccgcctgg cccgccgcgc
     1081 cttccgcaaa ctcccccgcc aagccgggca taggctaaat ttacggcttt ggcgccggtg
     1141 gcgaatgcag acacttcgcc gaccacaata atgacagaga ctctcccgcg cttcggcgcg
     1201 ggcagatcag gctgaagcaa gctgacttcg agatcgcgag acatgaacac catcagaaaa
     1261 ttgatcgccg ttgccacgac cttggccgtg ctcctgatcc tgacgctggt cgccgctgcc
     1321 tggttcggct tcgaccgtaa tctggacgcg ctcaggacag aacgggaggt gacccagccg
     1381 gcgctgcggg cgatgctgca agcccgtttc catgtggtgc agatccagca gtttctgacc
     1441 gatgtctcag cgaccggcga cgcgcaagct tacgaggaag ccaggcggaa cctgcagcag
     1501 gccaaggcgc atctggcacg cctggccgaa ctgaacccgt cgctggcggc cgagctcggt
     1561 cagttgcaga aggcactcga cgatttccat gcggccggcg tcgacatggc gcaggcctac
     1621 gtcagcgccg gacgcgaggc gggcaacgcg aagatgcagg cgccggaggt gggattcgac
     1681 gcccgggcgg tgaaactgac tgcccagttg gaccggatgg agcaggaaat cagcgaactc
     1741 gccgaacaag cggcggctac atcgctggac atgggcgcat cggcacggac gcaaaccttg
     1801 ttgcttggtc tcgtcgtcgc gctttgcgtc gtcggcgccg gccgccatct gcgccgcctg
     1861 ctctaccgcc agctcggcgg cgagccggcg ctggcggtcg ccgtggccaa ccgggtggcg
     1921 aacaatgagc tggatttcga catcccgcta acagccgaca acacggacag cctgatggcg
     1981 gccctcaagc gcatgcagcg aaacctgttg gcgcggacgc aggcggaaca cgagacagcg
     2041 caggagatgc tgcgcgtcaa gatcgccctc gacaacgtca gtaccggcgt gatgatcgcc
     2101 gatcccgaac gcaagatcat ctacgtcaac aaggcggtgc aggccgtact gaagaacgcc
     2161 gaggcggaca tccgccagca gttgcccaat ttcagcgccg accagctgat cggtcagaac
     2221 atcgacgccc tgcacaagaa tcccgcgctc caggccaggt tgctgggcga gttcacccgc
     2281 acccatagcg cccagctaca gatcggcgag cgccacatga cggtgaccgc caacccggtg
     2341 atcacggccg atgccgcacg cctcggcgcc gttgccgaat ggcgcgaccg gacgcacgaa
     2401 gtgcaggccc agggtgaaat ccgcagcatc ctcgacgccg ccgtccaggg cgatttcagc
     2461 cgccgcatca agacccgcga caaggagggt ttcttcctcg acatggcctt gggcatgaac
     2521 caattgctcg gcgtcgtgct ggaggcgctc gacgacgttg cccgcgtgct cagcggcctg
     2581 tcgcgcggcg acctgtcgca gcggatcgaa agcgattacg aaggcacgct cggtcagctc
     2641 aaggacgaca ccaacgccac caccgagcag ctacgttcgg tggtttccca gatcaaatac
     2701 gcggccgacg cgatcaatgt ggcagccaag gaaatcgcct cgggcaacca ggacctgtcg
     2761 caccggacgg aggaacaggc ctccagcctg gaagagacgg caagttcgat ggagcaattg
     2821 aacagcacgg tgcgcaacaa tgccgaactg gcgagcaagg ccgccgaact ggctgccaac
     2881 tcgaacctgg cggcggaacg cggcggtgcg atggtcggtc gggtggtcga cacgatgggc
     2941 ggcatccagg ccagttcgaa gaagatcgcc gacatcatcg gcgtgatcaa cagcatcgcc
     3001 ttccagacca acatccttgc cctcaacgcg gcggtcgaag cggcgcgtgc cggtgaacag
     3061 ggccggggct tcgctgtggt cgccaccgaa gtgcgcaatc tggcccagcg ctcggccgat
     3121 gcggcgaagg agatcaaagc gctgatcgac gaatcggtcg gcaaggtcga aggcggcgtc
     3181 caactggtac acgacaccgg cgagaccatc cgcgaggtgg tcgtcgcctt ccagcaactg
     3241 gcgcggctgg tcaacggaat tgccgaagcc agccgggaac aggccagcgg gatcgaacag
     3301 gtgtcgggcg ccgtctcgca gatggacgaa gtgacgcaac agaatgccgc cctggtcgaa
     3361 gaagcggcag cggcggcgga atcgctcgag gagcaggtcg agggcctgag ccgttcggtc
     3421 aacatcttca gcctggacgg caaagccctc accctgggtg gctcgggcgg ggtcgatttc
     3481 gacgcgatca tccaggctca cctgcaatgg aagttcaagc tgcagcaata cctcgacggc
     3541 aagggcgaag cgctcgaccc cgccgtggtc ggctgcgacg acaaatgcgc gatgggcaaa
     3601 tggatctatt cggacggcaa ggccagctac ggcaatgacc cgctgttcga ggaaatgcgg
     3661 cgcaaccacg cggctttcca ctgttgcgcc ggcgacgtga tccggctcgc gcaaaagggc
     3721 gaaaaggccg aggccgcgct acgcctgcgc gacgatttcg ccgcccagtc ggcgctcacg
     3781 gtaggccaga ttcgcgccat gaaaaagcgc cacgggcacg gtaaaacgct tgccccggta
     3841 ccgctggctc cggccgggaa acgcgctgcg ccacccagca ccgtgccggc cctggcgccg
     3901 gcacgacaaa gcggcgaaaa cgagtggatc gagttctgat cggtgccggg gccgccccga
     3961 cgcggccctt gcctcagtaa tcgcccgatg caccgccgcc gccggaactg ccgccgcccg
     4021 acgacgacga gtcgctgtcg ccagaacgcc acgacgagga cgacgaaacg ctttcccagt
     4081 tgctgtacgt cgtcccactg ccgccgccct ggccgggttt gccgaacagc gcgggcaagc
     4141 caaaaaccag gccgaacagg gcattggcac cgagcaagcc ccaggcgatt tccggcgcat
     4201 cggaaaaaac ctgcgaaaag acaccgaaga acacgccaaa gaaaaaccct aagaaggcaa
     4261 acaggacgaa agccgtgcgc gtcttgcgcc ccaaatcggt cgacttcggc acgcgccgcc
     4321 ggctgatcag ccagtaaagc agcggaaaaa gcaaattgcc cagccagagg acaagcaagc
     4381 gttgaaggaa aatagcgccg tcgagttgcg cgctctgctc agccgggtcg ccgccggtca
     4441 gttcggcgag cgccgccgga tcgccctgca tgctgcggcg catcgcctcg aagccggcga
     4501 gcaggccatc gggatagcga ttctcgcgga aagccggctg atagatgtcg atgacgatgc
     4561 gtttgctgag cgcgtcggtg agcgtgcctt ccagcccgta gccgacttcg atgcgcatct
     4621 tgcgctcgtt gggcgcgacc atgacgagca ggccgttgtc cttgtcggcc cggccgaggc
     4681 gccattgttc gaaggtcttg agcgccgcgt tctcgatgct gtcgccctgc aggctggcgg
     4741 cgatgaagac ggcggcccag atgccgtcct ggctgcgcag ttcggcgatg cgctggctga
     4801 gcgtcgcccg ttcttcggct gagagcagcc cggcctggtc aaggacatcg ccctgaaaag
     4861 ccggcggcgc gaaggcgcgg gcggcgaacg cgagcaagga caggctgagc gccagccaga
     4921 actggcgctg ccagcgcagg ggattcaggc gggcggcttg catggtttcg cttctcctca
     4981 gcccggctcg ggcctttcga tcaggctgcg cagcaactcg ccaccgggca gttgaaagct
     5041 gagcccgatc gcccgcactt gccagtcgcc accgggagtc cagcgctgga agccggtcag
     5101 cacgcagtcg ctgctgcgtt tctcgccggc ttcgggcggg ctgccgagca tctgcgtgta
     5161 gggcatcagc ccgggctgtt cgaggatcag gtaggcgcgc ggacggggaa tcggcaccac
     5221 cggatcgacg ccgtcggccc ggaaggcgac ggtgaattcg gcggccggcg gggcgttggc
     5281 gaaatccagc gccggcgtga attccgagcg ctgccggccg gcgaaacctt tttcccagtg
     5341 caagcgaagg taaggcggcg cctctttggc cgaccacatg aggtgcaggt cgttgccgtt
     5401 ggtgcagtaa accggatggg cgctgccctg atcgaccggc cacagccccc cttggcgcag
     5461 ttcgacggcg agcagccggg tatcggccgg cgccagcagg gactggctgt agatcagccc
     5521 gacgccgggc aggtggtagc gctgtaccgg ctgcacggtg tggcgcagcg ggatgttgtc
     5581 ggcgggtagc gtgcgcgcgc ggccgatctc gcgggcggcc gcggagagcg gcgcttcggc
     5641 ggcgaagaag gcgctgccgg gaatggccag gatggccagt ccgctgaccg ccagcgccag
     5701 gctctcgccg cccgtcagcg ctggcctcgc ccagcggccg gcgagcaggt ggctgccgaa
     5761 ccagagcggc ggggcgacca agagcagggc cacgtagaac accgccagcg cctccgggcc
     5821 gccgacgctg cgcgccggga cgatcagcac ggcaagcacc agcggggcgg ccaacgccga
     5881 caccaggcgc acgaagagcc agccggcgag gcgcccggaa cgcttgaagc agcggctttc
     5941 cagccagagc agagcaagca ccagggcgag caggccagcc gccgccagcc accagacctg
     6001 cctttgcccg gcgggcatgc gtgagaaggt atcgaggact tgctgccatt ccatggcggg
     6061 gctccggaaa atgccgcggg cgacgtgttc gccagtttaa ccgcgagcgg gaccggctgc
     6121 cggcacgcgc ggaagcgatg ccaggcccgg acgacggaaa ccgcgctacc catttccggc
     6181 aattcccggg acaattccgc ctcgttccgg aagcttcccc gtgcccaccc cgctccccgt
     6241 ctacctcgcc ggctaccccc ccagcctgac cgaaccggtc gcccgtctgc tcgcccaggg
     6301 gcggctgggc gaagcgctgc gccgcaaata cccgagcgcg cacacggtcc gcaccgaccg
     6361 ggcgctgcac gactacgtcc aggcgctgcg cgccgaccac ctgcgcaacg cgccgcagcc
     6421 gagcaagatc ctgttcgatg gcaagctgca gtcagtgggc cgggcgctcg gcacgcacac
     6481 gcggatcgcc cgcgtccagg gcggcaagtt gaagaccaag cacgaaatcc gcatcgccgc
     6541 gatcttccgc gaagcgccgc cggaattcct gcgcatgatc gtcgcccacg aactggcgca
     6601 tctgcgggaa ggcgaccacg acaaggcgtt ctacgccctc tgctgccaca tcgagccgga
     6661 ctatcaccag ctggagttcg atacccgggt ctatctctgc catcttgcgg cgggtggtac
     6721 accgttgtgg ccgacggctg tctgatttgc gacaagccac ccgacagcgg gccggcgaaa
     6781 ttttcgtaac aaactgattt aacagggaat acccccctgg cacagcgatt gctgaaatga
     6841 ctgcgaaccg atttccagga ggcagaccgt ggaacttcca gtcatcagca acaacgcacc
     6901 ggccgccgaa ggcggcggtt gttcatccag cggttgcggc acggcgccgg acgggctggc
     6961 tcacctgccg gaccacatcc gggccaaggt acaggaccat ccctgctatt ccgagcaggc
     7021 gcaccactac ttcgcccgca tgcacgtcgc cgtggcgccg gcctgcaaca tccagtgcaa
     7081 ctactgcaac cgcaagtacg actgctcgaa cgaatcccgc ccgggcgtcg tctccgagct
     7141 gctgacgccc gaccaggcga tcaagaaggt gctcgccgtg gccgccgaga tcccgcagat
     7201 gacggtactc ggcatcgccg gaccgggcga ccccttggcc aacccgggcc gcactttcga
     7261 cactttccgc cagctctccg agaaggcccc ggacatcaag ctgtgcgtgt cgaccaacgg
     7321 gctgtccttg cccgagcacg tcgacgagat cgccaagcac aacatcgacc acgtgacgat
     7381 caccatcaac tgtgtcgatc ccgaagtcgg cgccaagatc tatccgtgga tctactggca
     7441 gaacaagcgc atcttcggca tcgaaggcgc gaagatcctc atcgaacagc agcagaaggg
     7501 tctgcagatg ctgaccgagc gcggcatcct ggtgaaggtc aattcggtgc tgatccccgg
     7561 cgtcaatgac cagcacctca aggaagtctc gaagatcgtc aaggaaaagg gcgccttcct
     7621 gcacaacgtc atgccgctga ttgccgaagc cgaacacggc acctactacg gcatcatggg
     7681 ccagccggaa ccgagccagg aaatgctcca ggaactgcag gacgcctgtg ccggcgacat
     7741 ggcgatgatg cgccactgcc gccagtgccg cgccgacgcg gtcggcctgc tgggcgagga
     7801 ccgcggcgac gagttcacga tggacaagat cgacgtcatg gacatcgact acgaagccgc
     7861 catggtccgc cgcaagcagg tgcacgacga gatcatcgac aagctggaag ccgaacgcgg
     7921 cgtcgccaag ccgaagccga ccgacatccc ggaaggttcg cggccggtgc tgatggcggt
     7981 ggccggcaag aacggcctgg tcgccgagca cttcggccac gcccgcgagt tcctgatcta
     8041 cgaggcttcg ccggaaggtg tgcgcttcat cagccaccgc aagaccgagc tctactgcgg
     8101 cggcatggac gcctgcggcg acggcgacct ggtcgaagcg ggcggtacgg aatcgctgct
     8161 cgaccgcaac atccggattc tcgccggttg cgaggtcgtg ctctgctcca aggtcggcta
     8221 cgagccctgg gccaagctgg aagccgccgg catcgcgccg aacggcgaac acgcgatgga
     8281 accgatcgag gaagcggtga tggcggtgta caaggaaatt gcggcgaccg gcaaattgct
     8341 gccgcaagcc gatactctga aggtggcgta aatggcactg cagataaccg aatcctgcgt
     8401 caattgctgg gcttgcgtcg atgtctgtcc gaacgacgcg atctacgaag acaagccgca
     8461 cttcctgatc gagcccgata aatgcacgga atgcctgggc gaccacgccg acccgcaatg
     8521 cgcggcgatc tgcccgatcg agatggcgat cgtcaatgag ctgggcgagt tcctgaaccc
     8581 gcctggttcg ctgaccggca ttccgatcga gaagctgaag gaactgggat tgtggcggga
     8641 cgcggcgtaa ggggttcttc gccgctggcg gccgaccggg tttgggcctg gtcggctttg
     8701 ggcgcttctg gtgcgcggac cttgttggtt tctgaagcgc cggtttccgc gcctgacgcg
     8761 cgggcgtgct tggttgttga accgctgttt tccgccgctg accggcgggc gtactttctt
     8821 tttt
//
LOCUS       DAOSDX010000009         8649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3037_length_8649_cov_3.035606, whole genome shotgun sequence.
ACCESSION   DAOSDX010000009 DAOSDX010000000
VERSION     DAOSDX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3037_length_8649_cov_3.035606"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLN02_00390"
     CDS             complement(<1..356)
                     /locus_tag="PLN02_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI50809.1"
                     /translation="MTVLPTEPTPGELFFALRKPLNFASGEALLDVSLRLPPGQWLAL
                     QGPSGAGKTTLLRLLAGLETAAEGVIRAGDEIWLDSRRGSCLPTRLRRAGMVFQELGL
                     FPHMSARRQVDFARRP"
     gene            complement(337..528)
                     /locus_tag="PLN02_00395"
     CDS             complement(337..528)
                     /locus_tag="PLN02_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50810.1"
                     /translation="MADKPEPAADEAFAISPGAARAHGCCCGWRPAGRQPTAEIIDLA
                     KARAPIEATEEEVHDRPTD"
     gene            complement(545..1852)
                     /locus_tag="PLN02_00400"
     CDS             complement(545..1852)
                     /locus_tag="PLN02_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HOI50811.1"
                     /translation="MIRPWPMHRLAAGLCLAAVALAAAATPPRDLLTDPIPGMNGDET
                     ESFLRGRSLFRQSWVIAPARDQALDGLGPLYNRLACISCHPRNGRGGAPENPAQRMQT
                     MLVRLSVPGDPPRPHPVYGEQLNEEGIPGVPGEGRAEVHWQYETLSLDDGSRVELRRP
                     LLKFVDLAYGELGPVLTSSRVGPPVAGLGLLDAVPAATLAAMARETKADGVRGSVNRL
                     GPRRLAGRFGLKANSSDLRSQIAGAFHGDMSITSPLFPDENCSPAQTACRRAPDGGKP
                     ELSAAQLADITFYIAHLAPPARRDTEQPTVVRGNARFAEFGCAVCHRPTLQSGASRFR
                     LLAGRSFAPYTDLLLHDMGPGLADGRPDHAATGSQWRTAPLWGIGVLAAINEQVGYLH
                     DGRARNFEEAILWHGGEAAVAQRRYRAASADARAELLAFLQSL"
     gene            complement(1849..3138)
                     /locus_tag="PLN02_00405"
     CDS             complement(1849..3138)
                     /locus_tag="PLN02_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOI50812.1"
                     /translation="MNTLTLPSQQRRRLLKLAALGPLLGGALPRALAAPQVVTVVTSY
                     PDEFVSRVEAAFEAANPGYRLNVIWRNAADAADFLRQPEHGGADVYWSPSPRNFAQLA
                     QAGVWRPLPTERAALPPRIGKTPLGDHAGFYTASEVAGFGFAIVPDWFAKNAVAPPAD
                     WPDLTDPRLAGRIALPIPSQVGYAPPMVEIVLQAWGWDKGWALWSEIAGNATPIARAS
                     TFVSDLVADGRYAVGLSIDFFVNSAIANGHPVRFVYPAHTGLNPAHIAVTRAAPNPEG
                     ASRFVDFILSPAGQQLLRHADIRRLPVRPDAYTGAGFNPFAAAASGGMDFDAERARPR
                     LALSSAVFDAMLVTPHGELATLWQDLHRAEANGHDLGAVRRLLGTPPLDAAAADDPAL
                     QQRFAERDPTTTQLLTAWQRDCTTRRGEARRLLKALT"
     gene            complement(3140..5128)
                     /locus_tag="PLN02_00410"
     CDS             complement(3140..5128)
                     /locus_tag="PLN02_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI50813.1"
                     /translation="MQFVKHAIQPRLIAAVAAACALPAHADSSLFELGTVQVTGERSP
                     SGEIEAEQVSSVISAEDIERFSRTNVGDAVNLLSGVSISTNSRNEKMVYLRGYDARQA
                     PLFIDGIPVYVPYDGYVDMNRFTTADLSAIQVVKGFSSVAFGPNTLGGAINLISGKPT
                     RKFEGDVSLGIGSGHERRAALNVGTNQGLWYAQAGLSYDAADYFPMASGFSATTTENG
                     GHRENSAHRDSKVSLKLGLTPNATDEYALSYYRQDGEKGQPPSTDPAAARYWQWPYWD
                     KESLYFVSRTALGDKEMLKVRLFRDTFDNDLRIYSDASYTTYNPVSGVSVYHDKSHGA
                     SVELSSIRLPAQEIKLIASRKSDEHKSVDGAGATGEAFKDVLTSYAIEDNIRLGQRWL
                     LSLGLSRHSLEAEHVFKSGWPAFDISDEKRATDGQAALFFDLDAKNRLYASIAEKTRL
                     PTLKDRFSLRFGRYVENPDLKPESSINYEIGYQGTPWRALRVEAALFHSRIDDKIQSV
                     FLVPGTTACSNAKPCQMQNVGEVKSSGVELGLRSPLANWLDVGANYTYLRLKNVSDSS
                     IKITDVPRQKLSAFAIARFGDQLELLPYIESETGRWASNSKQLGGFTTVNLKLTYRPQ
                     PALALEAGVSNASDKQYETSDGFPSPGRIYYANLRYSF"
     gene            complement(5092..5424)
                     /locus_tag="PLN02_00415"
     CDS             complement(5092..5424)
                     /locus_tag="PLN02_00415"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOI50814.1"
                     /translation="MPFTPTPPADAPGIGELRRWLGVSQVEIAGLLGVSQPCVAKIEH
                     QEDMRLSTLRRYVAGLGGTLRLIAEIDGVAHELYIGAAPSAAEEKTSIHKNGGLQCNS
                     SNTLSSHA"
     gene            complement(5396..5782)
                     /locus_tag="PLN02_00420"
     CDS             complement(5396..5782)
                     /locus_tag="PLN02_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50815.1"
                     /translation="MYLPQPDPQRRLATDPEAAVDWLRLLPTEWREAVDRPLYFRRYR
                     EYEMLAERTIGYDEDARPCFITHQFTLTRLTSDDDEEFYETVALAEEMTAWRLHDGRW
                     LVLRNTQPDPGSKNGDFYAIHPDAPR"
     gene            complement(5958..6470)
                     /locus_tag="PLN02_00425"
     CDS             complement(5958..6470)
                     /locus_tag="PLN02_00425"
                     /inference="COORDINATES: protein motif:HMM:NF022115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HOI50816.1"
                     /translation="MMPSADPELPPIATIVAATGSADALLRDFVADLRRRGIRVRGLL
                     QESHADDSCCQHSLIDIEDGRRYPISQQLGSQSNACSLDTAALAEATAVMRRIADEGA
                     DLAIFNRFSKLESHGEGFAAEMLQVMSSGIPVLTVTSPSHLESWRHFTGGIARELPPD
                     AAALNDWFAA"
     gene            6549..7871
                     /gene="rimO"
                     /locus_tag="PLN02_00430"
     CDS             6549..7871
                     /gene="rimO"
                     /locus_tag="PLN02_00430"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOI50817.1"
                     /translation="MTQIQKVPTVGFVSLGCPKAGSDAELILTRLRAEGYEISPSYDD
                     SDLVIVNTCGFIDAAVEESLDAIGEALNENGKVIVTGCLGAKGDIVQTTHPSVLAITG
                     PHAMEEVMGHVHQHLPKPHDPYSDLVPPQGIRLTPAHYAYLKISEGCNHSCTFCIIPS
                     LRGPLVSRPVGDVLAEAENLARAGVKEILVISQDTSAYGVDLKYRTSFWGGRPVKTRL
                     KELCDALASFGIWVRLHYVYPYPSVDDVIPLMAEGKILPYLDVPFQHASPKILKAMKR
                     PGSSENTLERIARWREICPEIVIRSTFITGFPGETDEDFDQLIQFLEDAKLDRVGCFA
                     YSPVEGATANELPGLPPEDVREDRRRWLMQVQEDISADKLAAKIDTVIEVLVDEVDDE
                     GTIARSKADAPEIDGVVYLDGFFDAQPGDFLKVRVIDADHHDLYAQPA"
     gene            complement(7872..8219)
                     /locus_tag="PLN02_00435"
     CDS             complement(7872..8219)
                     /locus_tag="PLN02_00435"
                     /inference="COORDINATES: protein motif:HMM:NF022671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HOI50818.1"
                     /translation="MPLPVLAQHLARNRLSAYCEQRYPPELRESVRLDLRFEDDAVIL
                     YESRPSPQQAGQWIHLDIARFRYNLGSGTWLLDAPSFGGHSAWRQYSHRPERELRRLL
                     ALLDEDAEGVFWS"
     gene            8353..>8649
                     /locus_tag="PLN02_00440"
     CDS             8353..>8649
                     /locus_tag="PLN02_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOI50819.1"
                     /translation="MQALLDNLRELLGPDRVLGEPAAMAPMLTDWRGRYRGRALCVVQ
                     PGSSAEVAAVVRACVGAGAPIVPQGGNTSLCGAATPDDSGRAVVVSLSRMNRILA"
BASE COUNT         1320 a         2690 c         3158 g         1481 t
ORIGIN      
        1 tccgggcggc gggcgaaatc gacctggcgg cgggcgctca tgtgcggaaa caggccgagt
       61 tcctggaaga ccatgccggc gcggcgcagg cgggtgggca ggcagctgcc ccggcggctg
      121 tcgagccaga tctcgtcgcc ggcgcggatt actccttcgg cggccgtttc cagtccggcg
      181 agcaggcgta atagcgtcgt cttgcccgct ccggacggcc cctgcagggc cagccattgg
      241 ccgggtggca ggcgcaggct gacgtcgagc agcgcctcgc cgctggcgaa attgagcggc
      301 ttgcgcaggg cgaagaaaag ctcgccgggg gtgggctcag tcggtaggac ggtcatgcac
      361 ctcttcctcg gtggcttcga tcggcgctcg cgccttcgcc aggtcgatga tctcggccgt
      421 cggctgccgg ccagccggcc gccagccgca acagcagccg tgggcgcggg cggcgcccgg
      481 cgagatggcg aaggcttcgt cggcggcggg ctcgggtttg tcagccatga tttgctccgt
      541 gcggtcagag ggattgcagg aaggcgagca gctcggcgcg cgcatcggcg ctggcggcgc
      601 ggtaacgccg ttgcgccacc gccgcttcgc cgccgtgcca gaggatggct tcctcgaaat
      661 tgcgggcgcg gccatcgtgc aggtagccga cctgttcgtt gatcgccgcc aggacgccga
      721 ttccccacag cggcgcggtc cgccattggc tgccggtagc ggcgtggtcg gggcggccgt
      781 cggcgaggcc ggggcccatg tcgtggagca gcaggtcggt gtagggtgcg aaactgcgac
      841 cggcgagcag gcggaaacgg ctggcgccgc tttgcagtgt cgggcgatgg cagacggcgc
      901 agccgaactc ggcgaagcgc gcgttgccgc gcacgaccgt cggctgttcg gtgtcgcggc
      961 gcgccggtgg cgccaggtgg gcgatgtaga aggtgatgtc ggcgagctgg gcggcgctca
     1021 gttccggctt gccgccgtcc ggtgcgcggc ggcaggcggt ctgcgccggg ctgcagttct
     1081 cgtccgggaa gagcggcgag gtgatgctca tgtcgccgtg gaaagcgccg gcgatctggc
     1141 tgcgcaggtc actgctgttg gccttgaggc cgaagcggcc ggccaggcgc cgtgggccga
     1201 ggcggttgac gctgccgcgc acgccgtccg ccttggtttc gcgcgccatt gccgccagcg
     1261 tcgccgccgg caccgcgtcg agcaggccga gaccggcgac cggcgggccg acgcgcgacg
     1321 aggtgaggac cggccccagc tcgccgtagg caaggtcgac gaatttcagc agcggccggc
     1381 gcaactcgac gcggctgccg tcgtcgagac tcagcgtttc gtactgccaa tggacttcgg
     1441 cgcgtccttc gccgggcacg ccggggatgc cttcctcgtt cagttgctcg ccgtacaccg
     1501 ggtgcgggcg gggcgggtcg ccggggacgg agaggcggac cagcatcgtc tgcatccgct
     1561 gcgccgggtt ttccggcgcg ccgccgcggc cgttgcgcgg gtgacaggaa atgcaagcga
     1621 ggcggttgta gagcggtccg agaccatcca gcgcctgatc gcgggccggc gcgatcaccc
     1681 aactctggcg gaacaggctg cggccgcgca ggaagctttc ggtctcgtca ccgttcatgc
     1741 cgggaatggg gtcggtcagc aggtcgcgcg gcggggtcgc ggcggcggcg agtgccacgg
     1801 cagccaggca gaggccggcg gcgaggcgat gcatgggcca ggggcggatc atgtgagggc
     1861 tttcaacagg cgccgggctt cgccccggcg ggtggtgcag tcgcgctgcc aggcggtgag
     1921 cagttgcgtc gttgtcggat cgcgctcggc aaagcgttgc tgcaacgccg ggtcgtcggc
     1981 tgctgccgca tccagcggcg gcgtgccgag caagcggcgg acggcgccca ggtcgtggcc
     2041 gttcgcttcg gcgcggtgca ggtcttgcca gagcgtcgcc agctcgccgt gcggcgtcac
     2101 cagcatggcg tcgaagacgg cgctgctcag cgccaggcgc ggccgcgcgc gttcggcgtc
     2161 gaagtccatg ccgccgctgg cggctgcggc aaacggattg aagccggcgc cggtgtaggc
     2221 gtcggggcgc accggcaggc gccggatgtc ggcgtggcgc agcagttgct ggccggccgg
     2281 agagaggatg aagtcgacga agcggctggc gccttccggg ttgggggcgg cgcgggtgac
     2341 cgcgatgtgc gccgggttga ggccggtgtg cgcgggatag acgaagcgga ccggatggcc
     2401 gttggcgatc gccgagttga cgaagaagtc gatcgacagg ccgacggcgt agcgcccatc
     2461 ggcgaccagg tcgctgacga aggtcgaggc gcgggcgatc ggcgtcgcgt tgccggcgat
     2521 ctcgctccac aaggcccagc ctttgtccca gccccaggcc tgcaggacga tttcgaccat
     2581 cggcggcgcg tagccgacct gcgacggaat gggcagggcg atccgcccgg ccagccgcgg
     2641 gtcggtgagg tcgggccagt cggccggcgg cgccacggcg ttcttggcga accagtcggg
     2701 gacgatggcg aagccgaagc cggcgacttc gctggcggtg tagaagccgg cgtgatcgcc
     2761 gagcggcgtc ttgccgatac gcggtggcaa ggctgcgcgt tccgtcggca gtgggcgcca
     2821 gacgccggcc tgtgccagtt gcgcgaagtt gcgcggcgac ggcgaccaat acacgtcggc
     2881 gccgccgtgc tccggctgac gcaggaagtc ggccgcgtcg gcggcattgc gccagatgac
     2941 gttgaggcgg tagccggggt tggctgcctc gaaggcggcc tcgacgcgcg acacgaattc
     3001 gtccgggtag ctggtgacga cggtgaccac ctgcggcgcc gccagtgcgc gcggcagagc
     3061 gccgccgagc agcggcccga gggcggccag tttgagcagg cggcggcgct gctgggaggg
     3121 aagggtgagg gtgttcatct cagaagctgt agcgcaggtt ggcgtagtag atgcggcccg
     3181 ggctcgggaa accgtccgag gtttcgtact gcttgtcgct ggcgttgctg acgccggctt
     3241 cgagggcgag tgccggttgc ggacggtagg tgagcttcag gttgacggtg gtgaagccgc
     3301 cgagctgctt gctgttcgac gcccagcgac cggtttcgct ctcgatgtag ggcagcagtt
     3361 ccagttggtc gccgaagcgg gcgatggcga aggcgctgag cttctggcgc ggcacgtcgg
     3421 tgatcttgat gctcgagtcg ctgacgttct tcaggcgcag gtaggtgtag ttggcgccaa
     3481 catcgagcca gttggcgagc ggcgagcgca ggccgagttc gacgccgctg cttttcactt
     3541 cgccgacgtt ctgcatctgg cagggcttgg cgttgctgca ggcggtggtg ccaggaacca
     3601 ggaagaccga ctggatcttg tcgtcgatcc ggctgtggaa aagcgccgcc tcgacgcgca
     3661 gggcgcgcca cggcgtgccc tggtagccga tctcgtaatt gatcgacgat tccggcttga
     3721 ggtcggggtt ttcgacgtag cggccaaagc gcagcgagaa gcggtccttc agcgtcggta
     3781 gccgggtctt ctcggcgatg ctggcgtaga gacggttttt cgcgtcgagg tcgaagaaga
     3841 gcgcggcctg gccgtcggtg gcgcgctttt cgtcgctgat gtcgaaggcc ggccagccgc
     3901 tcttgaagac gtgttcagcc tccaggctgt gccgggagag gccgagcgaa agcagccagc
     3961 gctggccgag gcggatgttg tcctcgatcg catacgacgt caggacatcc ttgaacgcct
     4021 caccggtggc gccggcgccg tcgacgctct tgtgctcgtc gctcttgcgg ctggcgatca
     4081 gcttgatttc ctgcgccggc aggcggatcg acgacagttc gacggaagcg ccgtgactct
     4141 tgtcgtgata gacgctgacg ccgctgaccg ggttgtaggt ggtgtagctg gcgtcgctgt
     4201 agatgcgcag gtcgttgtcg aaagtgtcgc ggaacaggcg gaccttgagc atttccttgt
     4261 cgcccagcgc ggtgcgcgag acgaagtaga ggctttcctt gtcccagtat ggccactgcc
     4321 agtagcgggc ggccgccggg tcggtcgatg gcggctggcc tttttcgccg tcctggcggt
     4381 agtagctgag cgcgtattcg tcggtggcgt tcggcgtcag gccgagcttg agcgatacct
     4441 tgctgtcgcg gtgcgccgag ttctcgcggt ggccgccgtt ctccgtcgtc gtcgccgaga
     4501 aaccggaggc catcgggaaa taatccgccg cgtcgtagga cagcccggcc tgggcgtacc
     4561 acaggccctg attggttccg acgttgagcg cggctcgccg ttcgtggccg gaaccgatgc
     4621 cgaggctgac atcgccctcg aatttgcgcg tcggcttgcc cgagatcagg ttgatcgcgc
     4681 cacccagcgt gttcgggccg aaggcgaccg agctgaagcc tttgaccacc tggatcgccg
     4741 agaggtcggc ggtggtgaag cggttcatgt cgacgtagcc gtcgtaggga acgtaaaccg
     4801 ggatgccgtc gatgaacagc ggcgcctggc gggcgtcgta gccgcgcaga tacaccatct
     4861 tctcgttacg cgagttggtg ctgatgctga cgccggagag caggttgacc gcgtcgccga
     4921 cattggtccg gctgaagcgt tcgatgtctt cggcgctgat caccgaggac acctgttcag
     4981 cttcgatttc tccgctcggt gagcgctcgc cggtgacctg gacggtgccc agttcgaaca
     5041 gcgaagaatc ggcgtgcgcc ggtagcgcgc aagccgcggc gacggcggcg atcaggcgtg
     5101 gctggatagc gtgtttgacg aattgcattg aagacccccg tttttgtgaa tggatgtttt
     5161 ttcctcagcg gccgacggcg cggcgccgat gtacagttcg tgcgccacgc cgtcgatctc
     5221 ggcgatcagg cgcagggtgc cgccgaggcc ggcgacgtag cggcgcagcg tcgacaggcg
     5281 catgtcttcc tggtgttcga tcttggcgac gcagggttgg ctgacgccga gcaggccggc
     5341 gatttccacc tgcgaaacgc ccagccagcg gcgtagttcg ccgatgcccg gggcgtcagc
     5401 ggggggcgtc ggggtgaatg gcatagaaat ctccgttctt gctgccgggg tcgggctggg
     5461 tattgcgcag caccagccag cgtccgtcgt gcaggcgcca ggcggtcatt tcctcggcca
     5521 gggcgacggt ttcgtaaaac tcttcatcgt cgtcgctcgt caggcgggtc aatgtgaact
     5581 ggtgggtgat gaagcagggg cgcgcgtcct cgtcgtagcc gatggttcgc tcggcgagca
     5641 tttcgtattc gcgataacgc cggaaataca gcggccggtc gacggcttcg cgccattcgg
     5701 tgggcagcag tcgcagccag tcgaccgcgg cttccgggtc ggtggcgaga cggcgttgcg
     5761 ggtccggttg cggcagatac atcgttggtg cctcgtttgg aaatatagca acatatatag
     5821 cgattgacgg ctacacagca aaagtggtgc cagggtttta tggggtgttt cgctgctgaa
     5881 gcgtgttggc ttcgctacga tatgttgtag tgtatataac gattgtcggg taaccgccaa
     5941 tggcggtcaa taaaagctca ggcggcgaac cagtcgttca gcgcggcagc atccggcggc
     6001 agttcgcggg cgatgccgcc ggtgaagtgg cgccagcttt ccaggtggga gggggaagtg
     6061 acggtgagca ccggaatgcc gctgctcatc acctgcagca tctcggcggc gaagccttcg
     6121 ccgtggcttt ccagcttgga aaaacggttg aagatcgcca ggtcggcgcc ttcgtcggcg
     6181 atccgtcgca tcaccgcggt cgcctcggcg agtgccgcgg tgtccaggga acaggcgttc
     6241 gactggctgc cgagttgctg cgaaatcggg tagcgacggc cgtcttcgat gtcgatcagg
     6301 ctgtgctggc agcaactgtc gtcggcatgg ctttcctgca gcagtccgcg cacccgtatg
     6361 ccgcgccggc gcaggtcggc gacaaagtcg cgcaagaggg catcggcgct accggtggcg
     6421 gcgacgatgg tggcgatcgg tggcagttcg gggtcggcgc ttggcatcat ggcgcgattt
     6481 tacccggcgt ttccccataa aatacgcgtt ttctccgccg gccccgccgg tcaagtctgg
     6541 aatcccgcat gacacaaatt cagaaagtgc cgaccgtcgg tttcgtctcg ctcggttgcc
     6601 ccaaggccgg ctcggacgcc gaactcatcc tcacccgcct gcgcgccgag ggttacgaaa
     6661 tctcgcccag ctacgacgat tccgacctgg tcatcgtcaa tacctgcggc ttcatcgacg
     6721 ccgccgtcga ggaatcgctc gacgccatcg gcgaggcgct gaacgagaac ggcaaggtga
     6781 tcgtcaccgg ctgcctcggc gccaagggcg acatcgtcca gacgacgcac ccgtcggtgc
     6841 tcgccatcac cggcccgcac gccatggaag aggtgatggg ccacgtgcat cagcacctgc
     6901 cgaagccgca cgacccgtat tccgacctcg tgccgccgca gggcatccgc ctgacgccgg
     6961 cgcactacgc ctacctgaag atttccgaag gctgcaacca cagttgcacc ttctgcatca
     7021 tcccctcgct gcgcggcccg ctcgtctcgc gcccggtcgg cgacgtgctg gccgaagccg
     7081 agaacctggc ccgcgccggg gtcaaggaaa tcctcgtcat ctcgcaggac accagcgcct
     7141 acggcgtcga cctcaagtac cgcaccagct tctggggcgg ccggccggtg aagacccgct
     7201 tgaaggagtt gtgcgacgcg ctcgcctcct tcggcatctg ggtccgcctg cattacgttt
     7261 acccgtatcc ttcggtcgac gacgtcatcc cgttgatggc cgaaggcaaa atcttgcctt
     7321 acctcgacgt gcccttccag cacgccagcc cgaagatcct gaaggcgatg aagcgccccg
     7381 gttcgagcga gaacacgctg gagcgcatcg cccgctggcg cgaaatctgc ccggaaatcg
     7441 tcatccgttc gaccttcatc accggcttcc cgggcgagac cgacgaggat ttcgaccagc
     7501 tgatccagtt cctcgaagac gccaagctcg accgcgtcgg ctgtttcgcc tactcgccgg
     7561 tcgaaggcgc caccgccaac gaactgcccg gcctgccgcc ggaagacgtg cgcgaggacc
     7621 gccggcgctg gctgatgcag gtgcaggaag acatttccgc cgacaagctg gcggcgaaga
     7681 tcgacacggt gatcgaagtg ctggtcgacg aagtcgatga cgaagggacg atcgcccgct
     7741 ccaaggccga cgcgccggaa atcgacggcg tggtttacct cgacggcttc ttcgacgccc
     7801 agcccggcga tttcctcaag gtccgcgtca tcgacgccga ccaccacgac ctctacgccc
     7861 agcccgcctg atcaggacca gaagacgccc tcggcgtctt cgtcgagcag cgccagcagg
     7921 cggcgcagct cgcgctccgg acggtgcgag tactggcgcc acgccgaatg gccgccgaag
     7981 ctcggcgcat cgagcagcca ggtgccggaa ccgaggttgt agcggaagcg cgcgatgtcg
     8041 aggtggatcc actgcccagc ctgctgcggc gacggccgcg attcgtagag gatgacggcg
     8101 tcgtcttcga agcgcaggtc gaggcgaacg ctctcccgca gctcgggcgg atagcgttgc
     8161 tcgcaatagg cggagaggcg attgcgggca aggtgctggg cgaggacggg caggggcatg
     8221 ggagcgcggt cttggttatg aacgaatcat agcggctgga acgttgccgg gaaagcgttt
     8281 gacgcctcgc cgccgcctgc cgagaatgcg ctttgcgccg ccatcggcgg caattgacgg
     8341 aaatacgacg tgatgcaggc tttgctcgac aacttgcgcg aactgctggg accagaccgg
     8401 gtgctcggcg agccggccgc catggcgccg atgctgaccg actggcgcgg tcgttaccgc
     8461 ggtcgcgccc tgtgcgtggt ccagcccggc agcagcgccg aagtcgccgc cgtcgtccgt
     8521 gcctgcgtcg gcgccggcgc gccgatcgtg ccgcagggcg gcaacaccag cctgtgcggc
     8581 gcggcgacgc cggacgatag cgggcgggcg gtggtggtca gcctgtcccg gatgaaccgc
     8641 atcctcgcc
//
LOCUS       DAOSDX010000010         8598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3067_length_8598_cov_2.860939, whole genome shotgun sequence.
ACCESSION   DAOSDX010000010 DAOSDX010000000
VERSION     DAOSDX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_8598_cov_2.860939"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLN02_00445"
     CDS             <1..510
                     /locus_tag="PLN02_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOI50820.1"
                     /translation="VRRICRKMGMVFQQFNLFPHLTVLQNVIEAPLTVKGLSRDAIVP
                     RAEELLRKVGLLAKCGAYPSQLSGGQKQRVAIARALAMEPDIMLFDEPTSALDPELTG
                     EVLRTMRQLAEEHMTMLVVTHEMSFARDVSTEVIFMDHGEIVEARPAGEMFADPRHPR
                     TRAFLDHML"
     gene            complement(520..1646)
                     /locus_tag="PLN02_00450"
                     /pseudo
     CDS             complement(520..1646)
                     /locus_tag="PLN02_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1643..2107)
                     /locus_tag="PLN02_00455"
     CDS             complement(1643..2107)
                     /locus_tag="PLN02_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOI50821.1"
                     /translation="MPINWTSDLDTGIPVIDQQHRRIVDFINDLEAAQVLGNQEKIKQ
                     VIYDCIDYTLSHFAFEESMQEEAGYPYCKPHKKVHELFTRKVAEYQQRMELGDDVAEE
                     LHGMLARWLVNHIKRDDADYVASVRASMTPAPAAAPVEEKKTGGWLSRLFGR"
     gene            complement(2138..3394)
                     /locus_tag="PLN02_00460"
     CDS             complement(2138..3394)
                     /locus_tag="PLN02_00460"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOI50822.1"
                     /translation="MDIKETMQAIGRRARAASRRLATASTAEKNAALQAIADAIRREK
                     AALLAANAEDVAAARAAGLEPAMLDRLTLTEKGVDNMAEGVEQVAKLPDPIGEMGEFK
                     FRPSGIQVGKMRVPLGVIGIIYEARPNVTADAAALCLKSGNAAILRGGSEAIRCNQAI
                     AALVKEGLEFAGLPGDCVQVIETTDRAAVGELITMREFVDVIVPRGGKGLIARLLAES
                     RVPMIQHLDGNCHVYLDEDADPSKALKIVENAKTQRYGTCNTAESLLVDRSVAAMLLP
                     PIAHMLTAKGVEIRGCAETCAIVPNAVPASEDDYYAEFLAPIISVKVVSGIDEAIAHI
                     NQYSSKHTEAIVTDNHPKAMRFLREVDSASVMVNASTRFADGFEYGLGAEIGISTDKI
                     HARGPVGLEGLTSQKWVVLGDGHVRG"
     gene            3509..3748
                     /locus_tag="PLN02_00465"
     CDS             3509..3748
                     /locus_tag="PLN02_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50823.1"
                     /translation="MGEKLILPAEVKVCATCSYWDGERQVDEEMKLVVVADECQGHCL
                     VQETGKPALHDVRQECDCIWEDLGPDEVPPAGDTP"
     gene            complement(3745..4653)
                     /locus_tag="PLN02_00470"
     CDS             complement(3745..4653)
                     /locus_tag="PLN02_00470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI50824.1"
                     /translation="MTAPQCVLVVDDEKQNRDLLTELLHEECRVILAKNGAQALERAC
                     EQQPDLILLDVLMPEMNGFEVIQRLKNDDRTRHIPVIFISALDSPSDEERGLDLGAVD
                     YIAKPFHPPIVRKRVRNHLLSVHHRRLLENLAMIDSLTEIANRRRYDTQLESEWRRCA
                     RNGSPLSLMIIDVDHFKAYNDNFGHAAGDRVLRSIALAMSGQVGRPGDLVARYGGEEF
                     AILLPLTERRAARLMAEELRCLIEGLHLAHPHSLVAPVVTISIGGMTLTPTGGQVLPE
                     LFNRADAALYEAKRQGRNRVFWQDGD"
     gene            complement(4650..7247)
                     /locus_tag="PLN02_00475"
     CDS             complement(4650..7247)
                     /locus_tag="PLN02_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI50825.1"
                     /translation="MSRSPLNRLLPAPTIRNKLVALSFAFLLFAVGLVFLLVFTQQRQ
                     QLQAQWNDSMTAQARLLASNLQAAVAFADDREASRLLDSLAVNPTVLAARLVLADGKQ
                     LGAYRAANRSIPAFPENAGGSRFLDDVLVVRTPILLDEDQPAAAHVELLVSLEQYHLT
                     LQRTIRETSLALCVALVLALALTRFVVGRLMAPLEKLDRLANRISQDALLDERIATRR
                     NDEIGSLGQSFDRMLDSLQARDRELARYRESLESMVSQRTAELQQAIAEARDANRAKS
                     DFLARMSHEIRTPMNAIIGLGHMLSETPLAREQREFLEQIVQSSEALLGIINDILDYS
                     KIEAGSLTLERQPLEIAGVFHSVAALFALKARDKGLTLQFSGAEAIPERLLGDALRLS
                     QILINLVGNAIKFTERGSIRVGVRRLAGSTERVRLEFTVADSGMGIEREQLERLFSPF
                     TQADSSITRRFGGTGLGLSICRQLVTLMDGEIDVASTPGQGSVFRFTASLETAPASPP
                     APAPAGPRVTPRWQGEKILLVEDIPLNRKIAIALLQKVGLQVDIAGDGQQALDALARE
                     DYRLVLMDIQMPVMDGLTATRRIRADPRLRDLPVLAMTAHATREDSEQSRAAGINAHL
                     TKPIIPAVLYEAIARWLPPSGSAEAATETGGAGPALPAIPGIDTAQGLALHLQRNDFY
                     LKSLHAFRQDFADCDQQIDAALQAGDQASARRLAHSLKSVAASLGARELAEVARALEQ
                     TLSGDMPAPATAGNFAALAREHQRLLAALSGLPPLPESRRQTEKLAPAALEDLFSRLH
                     ASLQAGEADSRSCFDHLAAALPEHLPGRDIDMAKLLEEIGWLIDDVEYDTACARLQGL
                     REAWRKSLA"
     gene            complement(7244..7780)
                     /locus_tag="PLN02_00480"
     CDS             complement(7244..7780)
                     /locus_tag="PLN02_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HOI50826.1"
                     /translation="MPRFPHLRPAPHRRCWALLLALCCAAAMAQQPVSEYGMKAVLFF
                     RLSQFVYWPGQNGAPKPTVFCVAGDDNPFGDALAQIDKGNPTVEMRTTSGDLAGCHLL
                     FIPRAEAAALPVWLARTANRPLLTVSDIAGFARGGGMVELPLEGSRIGIVVNRRAALA
                     RGFEFNAQLLRLARVVEP"
     gene            complement(7788..>8598)
                     /locus_tag="PLN02_00485"
     CDS             complement(7788..>8598)
                     /locus_tag="PLN02_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI50827.1"
                     /translation="QLTPQWRVGAAYGTAFRAPSPEESYSAFGSFNNAGNPIGTFRAP
                     NANLLPEKSKTWSLTWDWRPTRRFNLVANAYLTEVDNVILTRRNDVPIQYIPGVVLSA
                     TESKQNSGSDRYSGFDLMPQWQVHLGGAWSADAWGSYSYVDGHFRETDDGPSLKQINI
                     AQHKLKLGVTLRYQDWLTITPRLQWIGETTTGAINRPTNDRVRKTDAYTVASLHLGIH
                     KLADDRLSLYLDIYNLFDKRYYAAHTSSSSAVMQAVPQQPRTVMATVEYRF"
BASE COUNT         1318 a         2674 c         3066 g         1540 t
ORIGIN      
        1 gtgcggcgca tctgccgcaa gatgggcatg gtcttccagc agttcaacct gtttccgcac
       61 ctgaccgtgc tgcagaacgt catcgaggcg ccgctgacgg tcaagggcct gagccgcgac
      121 gcgatcgtgc cgcgcgccga ggaactgctg cgcaaggtcg gcctgctcgc caagtgcggc
      181 gcctatccgt cgcagctatc gggcggccag aagcagcgcg tggcgattgc ccgggcgctg
      241 gcgatggagc cggacatcat gctgttcgac gaaccgacct cggcgctcga tcccgaactg
      301 accggcgagg tgctgcgcac gatgcgccag ttggccgagg aacacatgac catgctcgtc
      361 gtcacgcacg agatgagctt cgcccgcgac gtatcgaccg aagtcatttt catggatcac
      421 ggcgagatcg tcgaggcgcg gcccgccggc gaaatgttcg ccgacccgcg tcacccgcgc
      481 acccgcgcct tcctcgatca catgctctga agcgccgcct cagaacgccg agatgccggt
      541 ctgggcgcgg ccgaggatca gcgcgtggat gtcgtgcgtg ccctcgtagg tgttgaccac
      601 ttccagattg accaggtggc ggatgacgcc atagtcgtcg gaaatgccgt tgccgcccag
      661 catgtcgcgc gccgtgcggg cgatgtcgag ggccttgccg caggagttgc gcttcatcag
      721 cgaaacgatt tccggcgtcg cgctgccgtc gtccatcatc cggccgaggc gcagcgcgcc
      781 ctggatgccc agcgcgattt cggtctgcat gtcggcgagc tttttctgga tcaactggtt
      841 ggcagccagc ggccggtcga actgcttgcg ctccagcgtg tattgccgcg ccgtgtgcca
      901 gcaggcttcg gcagcgccca gcgcgcccca ggcgatgccg tagcgcgccg agttgaggca
      961 ggtgaacgga cctttcaggc cgccgacgtc aaggcggttt tcgctcggca cgaagacctc
     1021 gtccatgacg atgtcgccgg tgaccgaggc gcgcagcccg accttgccag caatcgccgg
     1081 cgcgctcagg ccggtcatcc ccttttccag gatgaaaccg cgcagcacgc cgtcgtcgtc
     1141 cttggcccag acgacgaaga cgtcggcgat cggcgcgttg ctgatccagc gcttgacgcc
     1201 ggacagccgc caaccgccgg cgacggcccg cgcccggcta cgcgcgctgg ccgggtcgga
     1261 accggagtcc ggctcggtca gtccgaaaca gccgatcagc tcgccgcgcc ccagtccctt
     1321 caggtatttc tctttctgtg tttcgctgcc gaaagcgaag atcggcagca tgaccagcga
     1381 cgactgcacg ctgagcatcg tccggtaggc cgaatcgacc gcctcgatgg cgcgggcgat
     1441 caggccgtag gagacgtagt tcagcccggc gccgccgtac tgcggcggca gcgtggcgcc
     1501 gagcagaccc atttcgccca tctcgcggaa caccgcgcga tcgaccgttt cctggcgaaa
     1561 gtctgcgccg cgttgcgtac ctggcgctcg tcggcggcga gctggctgtc gagacggaaa
     1621 gggtcgtccc aggcgaaacc gctcatcggc cgaacaagcg gctcagccag ccgccggtct
     1681 tcttttcttc gaccggcgcc gcggctggcg ccggtgtcat gctggcgcgc accgaggcga
     1741 cgtagtcggc gtcgtcgcgc ttgatgtggt tgaccagcca gcgggcgagc atgccgtgca
     1801 gttcctcggc gacgtcgtca cccagttcca tgcgttgctg gtattcggcg accttgcgcg
     1861 tgaacagctc atgcaccttc ttgtgcggct tgcagtaggg gtagccggcc tcttcctgca
     1921 tgctctcctc gaaggcgaag tgcgacagcg tgtagtcgat gcagtcgtag atcacctgct
     1981 tgatcttctc ctgattgccg agcacctgcg ccgcttcgag atcgttgatg aaatcgacga
     2041 tgcggcggtg ttgctggtcg atgaccggga tgccggtatc gaggtcggac gtccagttga
     2101 tgggcatgct gttgactccc cgtgattgtg tttgttttca gccgcggacg tggccgtcgc
     2161 ccaggacgac ccacttctgg ctggtcagcc cttcgaggcc gaccggaccg cgcgcgtgga
     2221 tcttgtcggt cgagatgccg atctccgcac ccaggccgta ttcgaagccg tcggcgaagc
     2281 gggtcgaggc gttgaccatg accgacgccg aatcgacttc gcgcaggaag cgcatcgcct
     2341 tcgggtggtt gtcggtgacg atggcctcgg tgtgcttcga cgaatactgg ttgatgtggg
     2401 cgatcgcctc gtcgatgccg gataccacct tgaccgagat gatcggcgcc aggaattcgg
     2461 cgtagtagtc gtcttcgctg gcgggcacgg cattcgggac gatggcgcag gtttcggcgc
     2521 agccgcggat ctcgacgccc ttggcggtca gcatgtgagc gatcggcggc agcagcatgg
     2581 cggccaccga gcggtcgacg agcagcgact cggcggtgtt gcaggtgccg tagcgctggg
     2641 tcttggcgtt ctcgacgatc ttcagcgcct tgctcgggtc ggcgtcctcg tccaggtaga
     2701 cgtggcagtt gccgtccagg tgctggatca tcggcacccg ggattcggcg agcaggcggg
     2761 cgatcaggcc cttgccgccg cgcggcacga tgacgtcgac gaactcgcgc atggtgatca
     2821 gctcgccgac cgcggcgcgg tcggtggtct cgatcacctg cacgcagtcg ccgggcagtc
     2881 cggcaaattc aaggccttcc ttgaccagcg cggcaatcgc ctggttgcag cggatcgcct
     2941 ccgagccgcc acgcaggatc gccgcgttgc cggatttcag gcagagggcg gcggcgtcgg
     3001 cggtgacgtt cgggcgcgct tcgtagatga tgccgatgac gccgagcggc acgcgcatct
     3061 tgccgacctg gatgccggac ggccggaact tgaactcgcc catttcgccg atcgggtcgg
     3121 gcagcttggc gacctgttcg acgccttcgg ccatgttgtc gacgcccttc tcggtcagcg
     3181 tcaggcggtc gagcatggcc ggctccagcc cggcggcgcg ggcggcggcg acgtcttcgg
     3241 cattggcggc gagcagtgcc gctttttccc ggcggatggc gtcggcgatc gcctgcaggg
     3301 cggcgttctt ttcggcggtg ctggcggtgg cgaggcgacg cgaggcggcg cgcgcccggc
     3361 ggccgatcgc ctgcatggtt tccttgatgt ccatggtgcg tttcgttcga agcaaaaggc
     3421 cattatagcc agacaaaatc acagggaact tccggaagcg ggtaaactct tatccgcatt
     3481 gattacgggg ctttccatcg aggacatgat gggcgaaaaa ctgatactac cggccgaggt
     3541 caaggtctgc gcgacctgca gttactggga cggcgaacgc caggtggacg aagagatgaa
     3601 actggtggtg gttgccgatg agtgccaggg ccattgcctg gttcaggaaa ccggcaagcc
     3661 agccctgcac gatgtgcgac aggagtgcga ttgcatctgg gaagacctcg gccccgacga
     3721 ggttccgccg gccggcgaca cgccctaatc gccgtcctgc cagaaaaccc ggttgcgtcc
     3781 ctggcgcttg gcttcgtaga gggcggcgtc cgcccggttg aacaattccg gcaggacctg
     3841 tccgcctgtc ggggtcaggg tcatgccgcc gatactgatg gtgaccaccg gcgccacaag
     3901 cgagtgcggg tgggcaaggt gcagaccttc gatcaggcaa cgtagttctt ccgccatcag
     3961 gcgggctgcg cggcgctcgg tcagcggcag caggatggcg aattcctcgc cgccgtagcg
     4021 ggcaaccaga tctcccgggc ggccgacctg accgctcatg gcgagggcga tgctgcgcag
     4081 gacccggtcg ccggcggcgt ggccgaaatt gtcgttgtag gccttgaaat gatcgacgtc
     4141 gatgatcatc agcgacagcg gcgagccatt gcgcgcgcaa cgccgccatt cactctcgag
     4201 ttgcgtgtcg tagcggcgcc ggttggcgat ctcggtcagg ctgtcgatca tcgccaggtt
     4261 ttccagcagg cgccggtgat gtacggaaag caggtggttg cgtacgcgct tgcggacgat
     4321 cggcggatgg aagggcttgg cgatgtaatc gacggcaccg aggtcgagcc cgcgctcctc
     4381 gtcgctcggc gagtcgagcg ccgaaatgaa gatcaccggg atgtgccggg tgcggtcgtc
     4441 gttcttcagc cgctggatga cctcgaaacc gttcatttcc ggcatcagca cgtcgagcag
     4501 gatcaggtcc ggttgctgtt cgcaggcgcg ttccagcgcc tgggcgccgt tcttggccag
     4561 gatgacgcga cattcctcgt gcagcagttc agtgagcagg tcgcgattct gtttttcgtc
     4621 gtcgacgacg aggacgcatt gcggtgcggt catgcgagcg atttcctcca tgcttcgcgc
     4681 aggccttgca gcctggcgca agccgtatcg tattcgacat cgtcgatcag ccagccgatt
     4741 tcttcaagca acttcgccat gtcgatgtcc cggcccggca agtgctccgg cagcgccgct
     4801 gccagatgat cgaaacagct tcggctgtcg gcctcgccgg cctgcaggct ggcgtgcagt
     4861 cgggaaaaca ggtcttccag ggcggctggc gccagttttt cagtttgcct ccgcgactcg
     4921 ggaagaggcg gcaggccgga cagcgccgcc agcaggcgct ggtgttcgcg ggccagcgcg
     4981 gcgaagttcc ccgccgtggc gggggccggc atgtcgccgc tgagcgtttg ctcgagcgcc
     5041 cgcgccactt ccgccagttc ccgggcgccg agcgaggcgg cgaccgattt cagcgagtgc
     5101 gctaggcgcc gcgcactcgc ctggtcgccg gcctgcagcg cggcatcgat ctgctggtcg
     5161 caatcggcga aatcctggcg gaaggcgtgc agcgacttca ggtagaagtc gttgcgctgc
     5221 aggtgcaggg ccagcccctg cgccgtgtcg atgccgggga tggccggcag cgccggcccc
     5281 gcgccgcccg tttcggtcgc tgcctcggcg ctccccgagg ggggcagcca gcgggcgatc
     5341 gcctcgtaca gcacggcggg gatgatcggt ttggtgagat gggcgttgat tccggcggcg
     5401 cgcgactgct cgctgtcctc gcgcgtcgcg tgagcggtca tcgccagtac cggcaggtcg
     5461 cgcaggcggg gatcggcccg aatccggcgg gtcgcggtga ggccgtccat caccggcatc
     5521 tggatgtcca tcaggaccag gcggtaatcc tcgcgtgcca gggcgtcgag cgcctgctgg
     5581 ccgtcgccgg cgatgtcgac ctggaggccg accttctgca gcagggcgat ggcgatcttg
     5641 cgattgagcg gaatgtcctc gaccagcagg attttctcgc cctgccagcg cggcgtgacg
     5701 cgcgggccgg ccggggccgg cgcgggtggg ctggcgggag cggtttccag gctggcagtg
     5761 aagcggaaca cgctaccctg gcccggggtg ctggcgacat cgatttcgcc gtccatcagg
     5821 gtgaccagct ggcggcagat cgacaggccc agcccggtgc cgccaaagcg ccgggtgatg
     5881 ctgctgtcgg cctgggtgaa gggcgagaac aggcgttcga gttgctcgcg ctcgatcccc
     5941 atgccgctgt cggcgaccgt gaattccagg cgcaccctct cggtgctgcc ggccagccgg
     6001 cgtacgccga cccggatgct gccacgttcg gtgaacttga tggcgttgcc gaccaggttg
     6061 atcaggatct ggctcaggcg cagggcgtcg ccgagcaggc gttcgggaat cgcttcggca
     6121 ccgctgaatt gcagcgtcag tcccttgtcg cgcgccttga gggcaaacag cgcggcgacc
     6181 gagtggaaga cgccggcgat ttccaggggt tgccgctcga gcgtcaggct gccggcttcg
     6241 atcttcgagt agtcgaggat gtcgttgatg atgccgagca gggcttccga agactgcacg
     6301 atctgctcga ggaattcccg ctgctcgcgc gccagcggcg tttcgctgag catgtggccg
     6361 aggccgatga tggcattcat cggggtgcgg atctcgtggc tcatgcgggc gaggaagtcc
     6421 gacttggcgc ggttggcgtc gcgggcttcg gcaatcgcct gctgcagttc ggcggtgcgc
     6481 tgcgacacca tgctttccaa cgattcgcgg taacgcgcca gttcccggtc gcgcgcctgc
     6541 agcgagtcga gcatgcggtc gaagctctga ccaaggctgc cgatctcgtc gttgcgccgg
     6601 gtggcgatgc gttcgtcgag cagggcatcc tgcgaaatgc ggttggccag gcggtcgagt
     6661 ttttccagcg gtgccatcag gcgaccgacg acgaagcggg tcagcgccag cgccagcacc
     6721 aaggcgacgc agagcgccag cgaggtttcg cggatcgtcc gttgcagcgt caggtggtac
     6781 tgctccagtg aaaccagcag ttcgacgtgg gccgccgccg gttggtcttc gtcaaggagg
     6841 atcggcgtgc gcacgaccag gacgtcgtcg aggaagcggg agccgccggc gttttccggg
     6901 aaggctggga tgctgcggtt ggccgcccgg taagcgccga gctgtttgcc gtcggcgagg
     6961 accaggcggg cggcaagcac cgtcggattg accgccagcg agtcgagcag gcggctggcc
     7021 tcccggtcgt cggcgaaagc gaccgccgcc tgcaggttgc tggccagcag gcgggcttgc
     7081 gcggtcatcg aatcgttcca ttgcgcctgc agttgctggc gctgctgggt gaagacgagg
     7141 aggaagacga ggccgacggc gaacagcagg aaggcgaagg acagcgcaac cagcttgttg
     7201 cggatggtcg gcgccggcag caggcgattc aggggcgacc ggctcatggt tcgaccaccc
     7261 gcgccaggcg cagcaactgg gcgttgaatt caaagccgcg ggcgagggcg gcgcgccggt
     7321 tgacgacgat gccgatgcgg ctgccttcga gcggcagttc gaccatgccg ccgccgcggg
     7381 caaagccggc gatgtcggaa accgtcagca gcggccggtt ggcggtgcgc gccagccaga
     7441 ccggcagggc cgccgcctcg gcgcggggaa tgaagagcag gtggcagccg gcgaggtcgc
     7501 cgctggtggt acgcatctcg accgtcgggt tgcccttgtc gatctgggcg agcgcgtcgc
     7561 cgaacggatt gtcgtcaccg gcgacgcaga acacggtcgg cttgggcgcg ccgttctggc
     7621 cgggccagta gacgaattgc gacagacgga aaaacagcac tgccttcatc ccgtattcgg
     7681 agaccggctg ctgcgccatg gcggcggcgc agcacagcgc aagcagcagc gcccagcagc
     7741 gacggtgggg ggcggggcga aggtgcggga agcgcggcac gccgggctca gaagcggtat
     7801 tcgaccgttg ccatcacggt tcgtggttgc tgcggcacgg cctgcatgac ggcgctcgac
     7861 gacgaggtgt gggcggcgta gtaacgcttg tcgaagaggt tgtagatgtc cagatagagc
     7921 gacaggcggt cgtcggccag cttgtggatg ccgaggtgca ggctggccac cgtataggcg
     7981 tcggtcttgc gcacccggtc gttggtcggc cggttgatcg cgccggtcgt cgtctcgccg
     8041 atccattgca ggcgcggcgt gatcgtcagc cagtcctggt agcgcagggt gacgccgagc
     8101 ttgagcttgt gctgggcgat gttgatctgc ttcaggctgg ggccgtcgtc ggtttcgcgg
     8161 aagtggccgt cgacgtagct gtagctgccc caggcgtcgg cgctccaggc gccgcccaga
     8221 tggacctgcc attgcggcat caggtcgaag ccgctgtaac ggtcgctgcc gctgttctgc
     8281 ttcgattccg tggcgctgag gacgacgccg ggaatgtact ggatcggcac gtcgttgcgg
     8341 cgggtcagga tgacgttgtc gacctcggtc aggtaggcat tggcgaccag gttgaagcgc
     8401 cgggtcggcc gccagtccca ggtcagcgac caggtcttcg atttttccgg gagcaggttg
     8461 gcgttggggg cgcggaaagt gccgatcggg ttgccggcgt tgttgaagct gccgaaggcg
     8521 ctatagcttt cttccggcga gggcgcgcgg aaggccgtgc cgtaggcggc gccgacgcgc
     8581 cattgcggcg tcagctgg
//
LOCUS       DAOSDX010000011         8114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3287_length_8114_cov_2.418166, whole genome shotgun sequence.
ACCESSION   DAOSDX010000011 DAOSDX010000000
VERSION     DAOSDX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_8114_cov_2.418166"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLN02_00490"
     CDS             <1..457
                     /locus_tag="PLN02_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOI50828.1"
                     /translation="MLPLALLVGAFIFLRGHNVPGGGFIAGLVVAIALIMQYMASGHG
                     WAAVRMKVDYHALIGIGVLTAAATGVVAWLADHPFLTSAFGHFHLPLVGEFELASAMA
                     FDTGVFLTVVGAVMLALANLSRIGVLADAPPPQDGPMEPDLSAPRHGDH"
     gene            444..794
                     /locus_tag="PLN02_00495"
     CDS             444..794
                     /locus_tag="PLN02_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HOI50829.1"
                     /translation="MGITDMEFLLSSAIGLLTAAGVYLVLRARTWPVVLGLALLSYAT
                     NLFLFATGRLVLNQPPVISASAAGYTDPLPQALVLTAIVISFGMTAVIVVMALRSFFE
                     NGSDHVDGKAEDAA"
     gene            791..2320
                     /locus_tag="PLN02_00500"
     CDS             791..2320
                     /locus_tag="PLN02_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HOI50830.1"
                     /translation="MNHWIIAPILLPAIMAPILLMSARYDIVLARVFSIAATVALLGI
                     ALALGSMVGDGQILSYALGNWVAPFGIVLVADRLSVMMLVLTALLALAVLFFAIDGGD
                     RDGRHFHALYQFQLMGINGAFLTGDFFNLFVFFEVLLIASYGLMVHGGGPARVKAGVQ
                     YVVINLVGSTLFLIAVGMIYSVAGTLNMADLALKVPQVAAADQALLQAGALLLLLVFC
                     IKAALVPVHFCLPGTYANAPAPVAALFAIMTKVGAYCVIRLYVLAFGEEAGAAAWLAA
                     PWLLPAALVTLTLGMIGVLAARRLGQLASFAVIGSMGMLLTGIALFTPQALSAALYYL
                     LHSTLAGAVLFLIADLIARRRPGLDDQLSPAPRFAQQSLLAAAFFVAAIAATGMPPLS
                     GFVGKLLILDAARDNLQATTIWALVLGTSLLAIIGFGRAGSTLFWKSTSQPGSFPAPA
                     SGNALALVAIGLLLAGNVALTLFAGPLTAYLDATAEQVFATHRYIEAVLGNTVLPGGL
                     L"
     gene            2320..2841
                     /locus_tag="PLN02_00505"
     CDS             2320..2841
                     /locus_tag="PLN02_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOI50831.1"
                     /translation="MAHPSAPRPTLLRRWFPHPLLSLMLTLIWLLLINGVSVGGLLVG
                     LVLGIAIPRLTGGFWPDRPGIRNYRRIFSYLLLVMWDIAVANLQVARIILFRPLASLH
                     TRWVCVPLALTSPEGIAVFAGTITMTPGTVSCDLAADGKSLLVHCLDAPDPEAAVRDM
                     KERYESRLLEIFP"
     gene            2838..3107
                     /locus_tag="PLN02_00510"
     CDS             2838..3107
                     /locus_tag="PLN02_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HOI50832.1"
                     /translation="MIEYAIHYAYVAISLGLLFNLWRLFVGPSTTDRVLAVDTMVINV
                     IALIIVYGIQTGSTMYFEAALLLAMFGFVSTVAYCKFILRGNIIE"
     gene            3117..3566
                     /locus_tag="PLN02_00515"
     CDS             3117..3566
                     /locus_tag="PLN02_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HOI50833.1"
                     /translation="MAWFNELLVSALIVIGASFALVGSWGLVRLPDLMSRLHAPTKAT
                     TLGVGGALVASMVYFLAGNGTLSVHELLITLFLFLTAPLTAHFLAKAWMHSQRPGDLP
                     QPAQRGWSTFDAGEAEREAASDQPRPPPLSDACGSSASPPPASPSSP"
     gene            complement(3508..3975)
                     /locus_tag="PLN02_00520"
     CDS             complement(3508..3975)
                     /locus_tag="PLN02_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOI50834.1"
                     /translation="MKRLSLLIAALGLSSAVLAGSADDVAVVDPYIRLAPPNATATAA
                     FMVLKNKGANEAKLVKVANPASKLSELHTHLNDNGVMRMRQIPEIVIPARGEAVLQPS
                     GLHIMLINMQAPLSEGQVVPLTLGFADGSSKSVEVKVTKAMPAAAMPMNHMHH"
     gene            complement(3992..4588)
                     /locus_tag="PLN02_00525"
     CDS             complement(3992..4588)
                     /locus_tag="PLN02_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOI50835.1"
                     /translation="MSERILAAIAAALAALILALLLFWHPDPGARPATPPNPLPAGGD
                     FTLQGAAGPVALHDYAGRLVLVYFGYTFCPDICPTTLNTVLEARALLTPEERQKVALL
                     FVSVDPQRDTPAHLKSYLEFFDPSFVGVTGEAPQIAEVAARYGVIYKLQPPGEGGNYA
                     VDHSAETFLVGPDGRLRARLAHGTPPAQMVALIRQYLN"
     gene            complement(4590..5045)
                     /locus_tag="PLN02_00530"
     CDS             complement(4590..5045)
                     /locus_tag="PLN02_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50836.1"
                     /translation="MNRKILLDLIGILLILLVVVVGYKLSPLLLPKSDLTVEPDAACN
                     LQEGACSASLPGGGRVTLTIGTRPIPLVKAFPLTVEAAGASRVEIDFAGVDMDMGFNR
                     PLLREDAPGLFSGEATLPVCVTGRMSWQATVLVDLPGSRVAIPFRFVTG"
     gene            5210..5701
                     /locus_tag="PLN02_00535"
     CDS             5210..5701
                     /locus_tag="PLN02_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOI50837.1"
                     /translation="MKQLLAALALALVANLSSAQPAPDSAPFFGATLANADDRPVAMS
                     RYRGKPLVVNFWARWCPPCRAEIPELNSFAKKHAGKIDVVGIGIEDDAAAVKAFARDF
                     PIQYPVYLAREQAHALMSGLGNARGGLPYTLFIDRNGKVIGGKLGMLRQADLDAAAPL
                     LLR"
     gene            complement(5908..8079)
                     /locus_tag="PLN02_00540"
     CDS             complement(5908..8079)
                     /locus_tag="PLN02_00540"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOI50838.1"
                     /translation="MAALTANLPEADVRRLRDAVDFAAQIYGNSLLGSGEGIWSHALG
                     MAVIIAGLRLDADSRIAALLFAVPAQDEQGVAHIEERFGKAVASLVYGIFRLNRLRPI
                     AKGFVAVDLEAGESNPAEMRAQVEVLRKMLLAMVEDIRVVLLRLASRTQTLRHYAAQP
                     DDLRVQVARETLELYSPLANRLGVWELKWELEDLSFRFIHPDIYKTIAKQLDEKRSER
                     EAFIADAVARVRAELAEAGIAKAEIYGRPKHIYSIWNKMRKKGVGFSEVYDVRALRII
                     VDDVKDCYTALGIVHNLWTPISKEFDDYISKPKGNNYRSLHTAVECPDGRSLEIQIRT
                     WDMHKHAELGVAAHWRYKEGAKAPGADAYDEKIAWLRQLLTWKNEVADSSDWVNHYKQ
                     AALDETIYVMTPQGRVVDLPSGATAVDFAYRVHTDLGHRCRGAKINGQLIPLNTPLET
                     GQQVEVLTAKQGGPSRDWLNPALGYIHTKSARIKVRAWFANLALEETLAEGRALVAKE
                     LQRAGQTGANIDELARRLDFAKADDLYIAAARGELNLRLFQAAARGEAAAETQLPDEV
                     QTRAPRKHSDGNQGILIVGVDKLLTQLARCCKPAPPDEIHGFITRGKGISIHRADCPN
                     FANLAALHPERVIETDWGSETTGTFACDIVVDAHDRQGLLRDISEIFTREKLNVVGVN
                     TQSRQGNAHMSFTVEIVNLRQLQRTLGLIHEVEGVVGVRRA"
BASE COUNT         1204 a         2805 c         2641 g         1464 t
ORIGIN      
        1 catgctgccg ctggcgctgc tggtcggcgc cttcatcttc ctgcgcggtc acaacgtgcc
       61 gggcggcggc ttcatcgccg gcctggtcgt cgccatcgcg ctgatcatgc agtacatggc
      121 gagcggccat ggctgggcgg cggtccggat gaaggtcgac tatcacgcgc tgatcggcat
      181 cggcgtgctg accgccgccg ccaccggcgt cgtcgcctgg cttgccgatc accccttcct
      241 gaccagcgcc ttcggccatt tccacctgcc gctggtcggc gaattcgaac tggcgtcggc
      301 gatggctttc gacaccggcg tcttcctcac cgttgtcggt gcagtgatgc tggcgctggc
      361 caacctctcg cgcatcggcg tcctcgccga cgcgccaccg ccgcaggacg gcccgatgga
      421 gcccgacctg agcgcaccgc gccatgggga tcactgacat ggaattcctg ctttcctcgg
      481 ccatcggcct gctcaccgcc gccggcgtct acctggtact acgcgcccgc acctggccgg
      541 tcgtcctcgg cctcgccctg ctctcctacg cgaccaatct cttcctgttc gccaccggcc
      601 gcctggtgct caaccagccg ccggtaatct cggcgagtgc cgccggctac accgatccct
      661 tgccgcaggc gctggtcctc accgccatcg tcatctcctt cggcatgacc gcggtgatcg
      721 tcgtcatggc cttgcgcagc ttcttcgaga acggcagcga ccatgtcgac ggcaaggcgg
      781 aggacgccgc atgaaccact ggatcatcgc gcccatcctg ctgccggcga tcatggcgcc
      841 gatcctgctg atgagcgccc gctacgatat cgtcctcgcc cgcgtcttct cgatcgccgc
      901 caccgtcgcc ctgctcggca tcgccctggc gctgggcagc atggtcggcg acggccagat
      961 cctgagctac gcgctcggca actgggtcgc acccttcggc atcgtcctcg tcgccgaccg
     1021 gctgtcggtg atgatgctgg tcctcaccgc gctcctcgcg ctggccgtgc tgttcttcgc
     1081 catcgacggc ggcgaccgcg acggccgcca tttccacgcc ctctaccagt tccagttgat
     1141 gggcatcaac ggcgccttcc tgaccggcga cttcttcaac ctcttcgtct tcttcgaagt
     1201 cctgctgatc gcctcctacg ggctgatggt gcacggcggc ggcccggccc gggtcaaggc
     1261 cggcgtccag tacgtggtga tcaatctggt cggctcgacg ctgttcctga tcgccgtcgg
     1321 catgatctac tcggtcgccg gcaccttgaa catggccgac ctggcactca aggtgccgca
     1381 ggttgctgcc gccgaccagg cgctgctgca ggccggcgcg ctgctcctgc tgctcgtctt
     1441 ctgcatcaag gcggcgctgg tgccggtgca tttctgtctg cccggcacct acgccaacgc
     1501 accggcaccg gtcgccgcgc tgttcgcgat catgaccaag gtcggcgcct actgcgtgat
     1561 ccgcctctac gtgctggcct tcggcgaaga ggctggcgcc gccgcctggc tggccgcgcc
     1621 ctggctgctg ccggcagccc tcgtcacctt gacactgggc atgatcggcg tcctcgccgc
     1681 ccgtcgtctc ggtcagctcg ccagcttcgc ggtgatcggc tcgatgggca tgctgctcac
     1741 cggcatcgcg ctgttcaccc cgcaagccct gtcggcggcg ctctactacc tgctgcattc
     1801 gacgctggcc ggcgccgtgc tgttcctgat cgccgacctg attgcccgcc gccgccccgg
     1861 cctcgacgac cagttgtcgc cggccccgcg tttcgcccag cagagcctgc tcgccgccgc
     1921 cttcttcgtc gccgccatcg ccgccaccgg catgccgccg ctgtccggct tcgtcggcaa
     1981 gctgctgatc ctcgacgccg cccgcgacaa cctgcaggcg acgacgatct gggcgctggt
     2041 gctgggcacc tcgctgctcg ccatcatcgg cttcggtcgc gccggcagca cgctgttctg
     2101 gaagagcacc agccaaccgg gaagcttccc cgccccggcc agtggcaatg cgctggcgct
     2161 ggtcgccatc ggcctgctgc tcgccggcaa tgtcgccctg acgctgttcg ccggcccctt
     2221 gaccgcctat ctcgacgcga cggcggaaca agtcttcgcc acccaccgct atatcgaagc
     2281 cgtactcggc aataccgttc tgccaggagg actgctctga tggctcatcc atccgcgcca
     2341 cgcccgaccc tgctccggcg ctggtttccc catccgctgc tcagcctgat gttgacgctg
     2401 atctggctgc tgctgatcaa cggggtctcg gtcggcgggc tgctggtcgg gctggtgctg
     2461 ggcatcgcca tcccgcggct gaccggcggc ttctggccgg atcgcccggg tatccgcaac
     2521 taccgccgga tcttcagcta tctcctgctg gtgatgtggg acatcgccgt cgccaacctc
     2581 caggtggcca ggatcatcct cttccgcccg ctggccagcc tgcacacgcg ctgggtctgc
     2641 gtgccgctgg cgctgaccag cccggaaggc atcgccgtct tcgccggcac catcacgatg
     2701 acgccgggca ccgtcagctg tgacctggcg gccgacggca agtcgctgct cgtgcattgc
     2761 ctcgacgcgc cggacccaga agccgctgtg cgcgacatga aggaacgtta cgaatcccgg
     2821 ctgctggaga tcttcccgtg atcgaatacg ccatccatta cgcctacgtg gcgatcagtc
     2881 tcggcctgct cttcaacctg tggcggctgt tcgtcggccc cagcacgacc gaccgggtgc
     2941 tggcggtcga taccatggtg atcaacgtca tcgcgctgat catcgtctac gggatccaga
     3001 ccggcagcac gatgtacttc gaagcggcac tgctgctcgc catgttcggt ttcgtgtcca
     3061 ccgtcgccta ctgcaagttc atcctgcgcg gcaacatcat cgaataggag aaaagcatgg
     3121 catggttcaa cgaactgctc gtctcggcgc tcatcgtcat cggcgccagc ttcgccctgg
     3181 tcggctcctg ggggctggtc cggctgcccg acctgatgag ccggctgcac gcgccgacca
     3241 aggcaacgac gctcggcgtc ggcggcgccc tggtcgcctc gatggtctat ttcctggccg
     3301 gcaacggcac gctatcggtg cacgaactgc tgatcaccct gttcctgttc ctgaccgcgc
     3361 cgctgaccgc ccacttcctg gccaaggcct ggatgcacag ccagcggcca ggcgatctgc
     3421 cgcaacccgc gcagcgcggc tggtcgactt tcgatgccgg cgaagcggag cgggaagcgg
     3481 cgagcgacca gccccggccg ccgccgctca gtgatgcatg tggttcatcg gcatcgccgc
     3541 cgccggcatc gccttcgtca ccttgacctc gaccgacttg ctgctgccgt cggcgaaacc
     3601 gagcgtcagc ggcaccacct gcccctcgct gagcggcgcc tgcatgttga tcagcatgat
     3661 gtgcaagccg ctcggctgaa gcaccgcttc gccccgggcg gggatgacga tttcgggaat
     3721 ctggcgcatg cgcatcacgc cgttatcgtt gaggtgcgta tgcagttcag agagtttcga
     3781 ggccggattc gccaccttga ccagcttcgc ctcattggcg cccttgttct tcaacaccat
     3841 gaaggcagcg gtggcagtgg cgttgggcgg cgccaggcgg atgtagggat caacgacggc
     3901 gacgtcgtcg gcgctgccgg caaggacggc gctcgacagg ccaagggcgg cgatcagcag
     3961 ggacaggcgt ttcatggcag ggctccttgt ttcagttcag gtattgacgg atcagcgcga
     4021 ccatctgtgc cggcggcgtg ccgtgcgcca ggcgtgcgcg caggcggccg tcggggccga
     4081 cgaggaaggt ttccgccgag tgatcgacgg cgtagttgcc gccctcgccc ggcggctgca
     4141 atttgtagat cacgccgtag cgagcggcga cttcggcgat ctgcggcgct tcgccggtaa
     4201 caccgacgaa gctggggtcg aagaattcga gatagctctt gagatgggcc ggcgtgtcgc
     4261 gttgcgggtc caccgagacg aagagcagcg cgaccttctg tcgctcttcc ggggtcagca
     4321 gcgcgcgcgc ttcgagcacg gtgttgagcg tcgtcgggca gatgtcgggg cagaaggtgt
     4381 agccgaaata gacgaggacg agacggccgg cataatcgtg cagggcgacc ggcccggccg
     4441 cgccctgcag cgtgaaatcg ccgcccgccg gcagcgggtt gggcggcgtc gccgggcggg
     4501 cgccgggatc gggatgccag aacagcagca aggcgagaat cagcgcggcg agcgcggcgg
     4561 cgatggcggc aaggatgcgt tcagacatgt cagcccgtca cgaaacgaaa gggaatggcc
     4621 acccgtgaac cgggtagatc gaccagcacc gtcgcctgcc agctcatgcg gccggtgacg
     4681 cagaccggca gcgtcgcctc gccggagaag aggcccggcg cgtcttcccg cagcaagggg
     4741 cgattgaagc ccatgtccat gtcgacgcca gcgaaatcga tctcgacacg gctggcgccg
     4801 gccgcctcga ccgtcagcgg gaaggccttg accagcggga tcggccgcgt gccgatagtc
     4861 agcgtgaccc gcccgccgcc gggcaggctg gccgaacagg cgccttcctg cagattgcag
     4921 gcggcgtcgg gttcgaccgt caggtccgac ttcggcagca gcagcggcga cagcttgtag
     4981 ccgacgacga cgaccagcag gatcagcagg atgccgatga ggtcgaggag tatcttcctg
     5041 ttcacttgtt tccttccgca tggacttgcc gccaggcgcc cggcaagacc ggcggcgatt
     5101 ctggcacagc cccgctggcg cccccctgcg acaaattgcc gcaggtcggc ggacagctca
     5161 tttatcaaat gctaatattc agcccctgcc catttttccc gaccgccccg tgaagcaact
     5221 tcttgccgcc cttgccctcg cccttgtcgc caacctgagc agcgcccagc cggcaccgga
     5281 cagcgccccc ttcttcggcg ccacgctggc caatgccgac gaccggccgg tggcgatgag
     5341 ccgctaccgt ggcaagccgc tcgtggtcaa cttctgggcc cgctggtgcc cgccctgccg
     5401 ggccgagatt cccgaactca acagtttcgc caaaaaacac gccggcaaga tcgacgtcgt
     5461 cggcatcggc atcgaggacg acgcggcggc ggtcaaggcc ttcgcccgcg acttcccgat
     5521 ccagtacccg gtctatctcg cccgcgaaca ggcgcacgcg ctgatgtccg gcctcggcaa
     5581 cgcgcgcggc ggtttgccct acacgctgtt catcgaccgc aacggcaagg tgatcggcgg
     5641 caagctgggc atgctgcgcc aggccgacct cgacgccgcg gcgccgctgc tgctgcgctg
     5701 accgctgccg ctaaggagcg ggcctgccgg ccatcccggg gaaacagcat ggtgatgccg
     5761 acgccgatcg ggatcggcgg ccaccaggtg ccgatcaact gaaagatgtt gacgtgaaag
     5821 aggtccaggt tggcaccgag ggcgacgacg ccgacggtga tcaggacgat ggcagcaacg
     5881 tttcccttca tcggggactc cctggattca ggcacgacgg acgccgacaa cgccttcgac
     5941 ctcgtggatc aggccgaggg tgcgctgcag ctgacgcaga ttgacgattt cgacggtgaa
     6001 gctcatgtgc gcgttgccct ggcgcgactg cgtattgacg ccgacgacgt tgagcttctc
     6061 gcgggtgaag atttccgaga tgtcgcgcag caggccctgg cggtcgtgcg catcgacgac
     6121 gatgtcgcag gcgaaggtgc cggtggtctc gctgccccag tcggtctcga tcacccgctc
     6181 cggatgcagc gcggcgaggt tggcgaaatt cgggcaatcg gcgcggtgga tcgaaatccc
     6241 cttgccgcgc gtgatgaagc cgtggatctc gtccggcggt gccggcttgc agcagcgcgc
     6301 gagctgggtc agcagcttgt cgacgccgac gatcaggatg ccctgattgc cgtcgctgtg
     6361 cttgcgcggc gcccgcgtct gcacttcgtc cggcagttgc gtctcggccg ccgcttcgcc
     6421 gcgcgccgcc gcctggaaca ggcgcaggtt gagttcgccg cgggcagcgg cgatgtagag
     6481 gtcgtcggcc ttggcgaaat cgaggcggcg cgccagttcg tcgatgttgg caccagtctg
     6541 cccggcgcgc tgcaattcct tggccaccag cgcccgccct tcggccagcg tctcttccag
     6601 cgccaggttg gcgaaccacg cccgcacctt gatccgcgcg ctcttggtgt ggatgtagcc
     6661 gagcgccgga ttgagccagt cgcgcgacgg cccgccctgc ttggcggtca gcacctcgac
     6721 ctgctgcccg gtctcgagcg gcgtgttgag cggaatcaac tggccgttga tcttggcgcc
     6781 gcgacagcgg tgaccgagat cggtatggac gcggtaggcg aaatcgacgg cggtcgcacc
     6841 tgacggcagg tcgacgacgc ggccttgcgg cgtcatcacg tagatggtct catcgagcgc
     6901 cgcctgcttg tagtggttga cccagtcgga gctgtcggcg acctcgttct tccaggtcag
     6961 caactggcgc agccaggcga tcttctcgtc gtaggcgtcg gcgcctggcg ccttggcgcc
     7021 ttccttgtag cgccagtgcg cggcgacgcc gagttcggca tgcttgtgca tgtcccaggt
     7081 gcggatctgg atttccaggc tgcgcccgtc cggacattcg accgcggtat gcagcgagcg
     7141 atagttgttg cccttgggtt tcgagatgta gtcgtcgaat tccttggaaa tcggcgtcca
     7201 caggttgtgc acgatgccga gcgcggtata gcagtccttg acgtcgtcga cgatgatgcg
     7261 cagggcgcgc acgtcgtaga cctcggagaa accgacgccc ttcttgcgca tcttgttcca
     7321 gatgctgtag atgtgcttcg gccggccgta gatctcggcc ttggcgatgc cggcctcggc
     7381 cagctcggcg cggacgcggg cgacggcgtc ggcgatgaag gcctcgcgct cgctgcgctt
     7441 ctcgtcgagc tgcttggcga tcgtcttgta gatgtcgggg tggatgaagc ggaacgacag
     7501 gtcctccagt tcccacttca attcccagac gccgagccgg ttggcgagcg gtgaatagag
     7561 ttccagcgtc tcgcgcgcca cctgcacgcg caggtcgtcg ggctgcgccg cgtaatggcg
     7621 cagggtctgc gtccggctgg ccagacgcag gaggacgacg cggatgtcct cgaccatggc
     7681 cagcagcatc ttgcgcagga cctcgacctg ggcccgcatc tcggccgggt tgctctcgcc
     7741 ggcttccagg tcgacggcga cgaagccctt ggcgatcggc cgcaggcggt tgagccggaa
     7801 gatgccatag accaggctgg cgaccgcctt gccgaaacgc tcctcgatgt gggcgacacc
     7861 ctgctcgtcc tgcgccggca ctgcgaagag cagcgcggca atgcgcgaat cggcgtcgag
     7921 ccgcagaccg gcgatgatca ccgccatgcc cagcgcgtgc gaccagatgc cctcgccgct
     7981 gccgagcagg ctgttgccgt aaatctgcgc ggcgaaatcg acggcgtccc ggagacgccg
     8041 gacatcggct tccggcaagt tggccgtgag cgcggccagg aactgttcag agtcgccgtg
     8101 ggcggcgacg gaat
//
LOCUS       DAOSDX010000012         7878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3392_length_7878_cov_2.733095, whole genome shotgun sequence.
ACCESSION   DAOSDX010000012 DAOSDX010000000
VERSION     DAOSDX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3392_length_7878_cov_2.733095"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2236)
                     /locus_tag="PLN02_00545"
     CDS             complement(<1..2236)
                     /locus_tag="PLN02_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI50839.1"
                     /translation="MTSVSALRLSDIASTAVLTVPPDLPLSAAVGRFSGQHVSSLIVV
                     ADGRPLGILTEWDLLRLLRQGASGEATVAEVMSAPLITARPEMDFAAAQLLMNSRGIR
                     HLVVVDGDGRLFGIVSESDFRRQLNQDLFELIRDLKAITERAEPMVEPACPMQAVLEL
                     MAERRLDHVLVGRDGDAEGIITERDLPRLIAVWGDLDRLTAGDVMSSPLHAVDEDVSV
                     AEAARRMDVEQLRHLAVRARDGRLIGVVSQHRLLERLGSVLAEKGRSRLAGQLDMVLE
                     ATGVGTWEYDHRRDLLTRSVALNSVLQIARDNVFEAFDEALERIEPADRERVSAAFRG
                     GLLGPDGQFAVDFRARDIAGGVRWFSSRGKVIERDSEGRPLFSAGVSIDIDAQKRHES
                     ELQRSEARFRSLLENAPLPMFYIDAGQRIVFTNRRFHQLFGYAAAEIPDLLAWAQRAF
                     PDPAYRAAIDRQWQATLRAVKSGGELRQLECDVTCRDGQVRSVGISAAMLGDDCLASF
                     IDFTEQRFQQRLLEFGNVILHLISIAAPLPDVLANICRHIEAAGSSLHCAVMLLDEDG
                     VHLRPGAAPSLPAAYGETIDGMEIGPAAGSCGTAAFLRKDVFVADIVGDPLWSNYRTL
                     AEKFGMAACWSSPILATDGSVLGTFAIYWPRPAPTISHTVRRHVEAATTLAAIAIEST
                     RREAAMLSMLEERRQAGALVRRLSQAIEQSPVAVVITDLEARIEYVNQAFVRVSGYSA
                     EELLG"
     gene            complement(2377..3558)
                     /locus_tag="PLN02_00550"
     CDS             complement(2377..3558)
                     /locus_tag="PLN02_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI50840.1"
                     /translation="MLSSMFHRRPVAVIALAQLFGTSLWFSANSAADDLARDWGIGAG
                     GIGALTAAVQLGFITGTLVFALSGLADRFSASRIFAVSAVLGAVGNALFAAGASGLGS
                     GLPLRFLVGVCLAGIYPLGMKLVVSWVPERAGSALAWLVGMLTLGSALPHGIRFLGSE
                     LAWPWPVAASSLLALLAAGLILRLGDGPHLPAARRGARLHWGRVVEAFAIPEFRASAF
                     GYFGHQWELYAFWTLVPMLLVGSGVVPAGSAALSGLAFAVVAVGGLACVLGGYWSRRI
                     GSARVAAWALAGSACCCALFPLLAGGPSWLPLLLLLAWGFCVVADSPHFSALSARACP
                     PAMVGSALAFQTAIGFAVTLPAIHLGSALVDTLGVGVAWLLLPGPLLGLVGLAPLWRR
                     G"
     gene            3694..4782
                     /gene="trmA"
                     /locus_tag="PLN02_00555"
     CDS             3694..4782
                     /gene="trmA"
                     /locus_tag="PLN02_00555"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - S-adenosylmethionine-dependent
                     tRNA (m5U54) methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HOI50841.1"
                     /translation="MPLPDLDPARYDQQLAARLAAFAADFAACELPPPTAHASQPLHY
                     RLRAEFRAWHHDGVWDYAMFDPENPRQPVLMRDFPAADAAICALMPRLRDATLASETL
                     QRRLYAVEFLATLSGDLLLTLIYHRRLDADWEAAARALAGTLGIAIIGRSKGQKLVLE
                     RDWVDESFELDGRQLHYRQYEGSFSQPNGGVNRQMLSWARTQAAGLGGDLLELYCGNG
                     NFTVALAPLFRRVLATEVSKTSVKAALHNLERNAIANTAVVRMASEELSAALAGRESF
                     QRLREIDLASHDFSTLFVDPPRSGLDPATLEIAAGFANILYISCNPQTLRDNVAALQA
                     THRIAAAAVFDQFPYTHHLECGVLLQRR"
     gene            complement(4793..6181)
                     /gene="trkA"
                     /locus_tag="PLN02_00560"
     CDS             complement(4793..6181)
                     /gene="trkA"
                     /locus_tag="PLN02_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOI50842.1"
                     /translation="MNILILGAGQVGASLAEALVSEANDITLVDLDAGALAQLADRLD
                     VRTVTGNAAFPSVLAEAGVETADLIVAVTQSDQSNLVACKLAHSRFAVPTRIARLRSP
                     DFLDPTLLSTDNFAVDFALCPEQEISDYIGKLVEYPEALQVVEFAEGRLTMVGLRAYE
                     GGLLVGKPIRAMRGHLPSGVDARIAAIFRDTRAINIDGDTVVQSGDEVFLLAATEHIR
                     TVLKELRRMQKPVRKVMIAGAGNVGARVAGRLEGSCEVKLVESRPARAALVAGWLNRT
                     LVLTGEATDETLLAREEIGSMDMFLALTNDDENNIMAASLAKRLGCRRVLALINREAY
                     ADMVQGGPIDIGLSPAQVSIGGLLTHVRQADVARVHSLRRGAAEALELVVRGERGSSR
                     VVGRRIEELPEIHGASIAAVVRGGRVIIPHHDTLIEAGDHVVVFCTRKRYIAAVEKLF
                     LPTASLLPGWFR"
     gene            complement(6198..7652)
                     /locus_tag="PLN02_00565"
     CDS             complement(6198..7652)
                     /locus_tag="PLN02_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOI50843.1"
                     /translation="MNRYAPVLRALGMVIMLFALTLLPPLFLSWAADDGAQAVYDEVF
                     ALTLLCGLYLWYRYRNVKREMNVRDGFLMVVLVWTVLPAFAALPFMLQLGISHTDAYF
                     EAMSGLTTTGATVLSGLDQLPLSINLWRHELVWVGGMGLIVLAIAILPLLGIGGRQMF
                     KAETPGPMKDAKMTPRIASTAKGLWVVYVGVTILCVLGYRVAGMGWFDAVCHAFSTMG
                     LGGFSTHDASYGYFNSPAIEAVAIVFMLIAGMNFGTLFVAVSRGSLKPYLNDPEAGWF
                     LGVCLVSVLGIALFLRANEIYPDFAEALRHSAFNVISIATTTGYASVDFAQWPLFAPL
                     WMLFLCSFATSAGSTGGGIKMARALLLYKQVYREIVRALHPNAVYSVRIGGQVAPSSI
                     LFAVLAFGFMYMVSIVSLTLVMVFTGLDVITAFTAVVASVNNTGPGLGEVGPSTTFAV
                     LEDFQTWVCIFAMLLGRLEIFTLLVVLTPAFWRR"
     gene            complement(7649..>7878)
                     /locus_tag="PLN02_00570"
     CDS             complement(7649..>7878)
                     /locus_tag="PLN02_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI50844.1"
                     /translation="PENVLRFFVYLLAHPGQVLTQRKLLAEIWGASYVESSHYLRVYV
                     GHLRQKLEDDPTQPRHFLTETGVGYRFQPDA"
BASE COUNT         1422 a         2711 c         2665 g         1080 t
ORIGIN      
        1 gccccagcaa ttcctcggcc gaataaccac tgaccctgac gaaagcctgg ttgacgtact
       61 cgatgcgcgc ttccagatcg gtgatgacga ccgccaccgg actctgctca atcgcctgcg
      121 acaggcgtcg caccagtgcg ccggcctggc gccgttcttc gagcatggac agcatcgccg
      181 cctcgcgccg ggtactttcg atggcgattg ccgccagcgt cgtcgccgcc tcgacatgac
      241 ggcgcacggt gtggctgatc gtcggcgccg gccttggcca gtagatggcg aaagtgccga
      301 gcacactccc gtcggtggcg aggatgggcg acgaccagca ggccgccatg ccgaattttt
      361 cggccagggt gcggtaattc gaccacagtg ggtcgccgac gatgtcggcg acgaagacgt
      421 ccttgcgcag gaaggcggca gtgccgcagg aaccggcggc agggccgatt tccatgccgt
      481 cgatcgtctc tccataggcg gccggcaggc tgggcgccgc ccccggtcga agatggacgc
      541 catcctcgtc gagcagcatc acggcgcaat gcagactgct gccggcggcc tcgatgtgcc
      601 ggcagatgtt ggccaggacg tcgggcagcg gcgcggcgat cgagatcagg tgcaggatga
      661 cgttgccgaa ttcgagcagg cgttgctgga aacgctgttc ggtgaagtcg atgaacgagg
      721 ccaggcaatc gtcgccgagc atcgccgccg agatgccgac gctgcgcacc tggccgtcgc
      781 ggcaggtgac gtcgcattcg agctggcgca gttcgccgcc acttttcacg gcacgcagcg
      841 tggcctgcca ttgccggtcg atcgccgccc ggtaggccgg gtccgggaag gcgcgctgtg
      901 cccaggccag gagatcggga atttccgccg cggcgtagcc gaacaactgg tggaaacggc
      961 gattggtgaa cacgatgcgc tgcccggcgt cgatgtagaa catcggcagc ggcgcgttct
     1021 cgagcaggct gcgaaagcgc gcctcgctgc gctgcagttc gctttcgtgg cgtttctggg
     1081 cgtcgatgtc gatcgacacg ccggccgaaa acagcggccg gccctcactg tcgcgctcga
     1141 tcaccttgcc ccgggagctg aaccagcgca cgccaccggc gatgtcgcgg gcccggaaat
     1201 cgacggcgaa ctgcccgtcc ggccccaaca gcccgccgcg aaaagcggcg ctgacccgct
     1261 cgcggtcggc cggttcgatc cgctccaggg cctcgtcgaa ggcctcgaaa acgttgtcgc
     1321 gagcgatctg caagacgctg ttgagtgcga cgctacgcgt cagcaggtca cgccgatggt
     1381 cgtattccca ggtgccgacg ccggtcgcct cgagcaccat gtcaagctgg ccggccagac
     1441 ggctgcgccc cttctcggca agcaccgaac cgaggcgttc gagcaagcgg tgctggctga
     1501 cgacgccgat caggcggccg tcgcgcgccc gcaccgcgag gtggcgcaac tgctcgacgt
     1561 ccatgcggcg cgccgcctcg gccaccgaaa cgtcctcatc gacggcgtgc agcggcgacg
     1621 acatgacgtc gccggcggtc agccggtcga gatcgcccca gacggcgatc agccgcggca
     1681 ggtcgcgctc ggtgatgatc ccctcggcgt caccgtcgcg gccgaccagc acgtgatcga
     1741 ggcggcgttc agccatcagt tcgaggaccg cctgcatcgg gcacgccggc tcgaccatcg
     1801 gttcggcccg ttcggtgatc gccttcaggt cacggatcag ctcgaacagg tcctgattga
     1861 gctggcgccg gaaatcgctt tcgctgacga taccgaacag gcgaccgtcg ccgtcaacga
     1921 cgacgagatg gcggatgccg cggctgttca tcagcaactg ggcggcggcg aaatccattt
     1981 ccgggcgcgc ggtgatcagc ggcgcgctca tcacctcggc caccgttgcc tcgccggacg
     2041 ccccctggcg tagcaggcgc agcaaatccc attcggtgag gatgccgagc ggccggccat
     2101 ccgcgacgac gatcagcgac gaaacgtgtt gtccggagaa gcgcccgacc gccgccgaca
     2161 gcggcagatc aggcggaacg gtcagcaccg cggtgctggc gatatcgctc aggcgtagcg
     2221 cggaaacaga agtcatggcg gcaggaaccc cggcaaatcc tgatcgcact tccgcgacgg
     2281 acacggccac cgggccgggt gcgaagtgca aagcggcatt ctagccccaa gccggcgcca
     2341 atgccgcctt tcgtcatagc taccggacag ccgccctcac ccccgccgcc acaacggcgc
     2401 cagaccgacc aggccgagca ggggaccggg cagcaacagc caggcgacgc cgacacccag
     2461 cgtatcgacc agcgcgctac cgagatggat ggccggcagc gtcaccgcga agccgatcgc
     2521 cgtctggaag gcgagcgcgc tgccgaccat ggccggcggg caggcgcgcg ccgagagcgc
     2581 cgaaaaatgc ggcgaatcgg ccaccacgca gaagccccag gcgagcagca gcaacagcgg
     2641 cagccaggac ggcccaccgg ccagcagcgg aaacagcgcg cagcagcagg ccgagccggc
     2701 cagcgcccag gccgcgacgc gggcgctgcc gatgcgccgg ctccagtagc cacccagcac
     2761 gcaggccagc ccgccgaccg cgacgacggc gaaagccagc ccggacaagg cggcgctgcc
     2821 ggccggcacg acgccgctgc cgaccagcag catcggcacc agcgtccaga acgcgtaaag
     2881 ctcccactgg tggccgaaat agccgaaggc ggaagcgcgg aactcgggga tggcgaaggc
     2941 ctcgacgacc cgcccccagt gcagccgcgc accgcgccgg gcagccggca agtgcggccc
     3001 gtcgccgagg cgcaggatca gcccggcggc gagcaaggcc agcagcgacg aggcggcgac
     3061 cggccacggc caggcgagtt cgctgccgag gaagcggatg ccgtgcggca gcgcactgcc
     3121 cagggtcagc atgccgacca gccaggccag cgcgctgcct gcccgctcgg gaacccagct
     3181 cactaccagc ttcatcccca gcggatagat gccggccagg cagacgccga ccaggaagcg
     3241 cagcggcagg ccgctgccga gcccgctggc gccggccgcg aacagcgcgt tgccgacggc
     3301 acccagaacg gcgctgacgg cgaagatgcg gctggcggaa aaacggtcgg ccagtccgct
     3361 gagggcgaag accagcgtgc cggtaatgaa gccgagctgg actgcggcgg tcagggcgcc
     3421 aatgccgccg gcgccgatgc cccagtcgcg cgccagatcg tcggccgcgc tgttggcgga
     3481 aaaccatagc gaggtgccga atagctgggc cagcgcgatg accgccaccg gtcggcgatg
     3541 aaacattgag gaaagcatgg cacgaatcat aatccggatt gcctgacggg ccgcttaccg
     3601 gtaacgcccg tcccggcacg ccggctttgc cggacggggg cccggcgcgg tatagtccgc
     3661 cgttttccgt cacgccgtcc cgagcgccgc ccgatgcccc tgcccgacct cgaccctgcc
     3721 cgctacgacc agcaactggc cgccaggctc gccgcctttg ccgccgattt cgccgcctgc
     3781 gagctgccgc cgccgaccgc ccacgcctcg cagccgctgc actaccggct gcgcgccgag
     3841 ttccgcgcct ggcaccatga cggggtctgg gactacgcca tgttcgaccc ggaaaacccg
     3901 cgccagccgg tactgatgcg cgactttccc gccgccgatg cggcgatctg cgcgctgatg
     3961 ccgcgcctgc gcgacgccac cctggccagc gagaccttgc agcgccggct ctacgccgtc
     4021 gaatttctcg ccacgctgag cggcgacctg ctgctcaccc tgatctacca ccgccgcctc
     4081 gacgccgatt gggaagccgc cgcgcgcgcg ctcgccggca ccctgggcat cgccatcatc
     4141 ggccgcagca aggggcagaa gctggtgctc gaacgcgact gggtcgacga aagtttcgaa
     4201 ctcgacggcc gccagttgca ctaccggcaa tacgaaggca gcttcagcca gccgaacggc
     4261 ggcgtgaacc gccagatgct gtcctgggcg cggacccagg ccgccggtct gggtggcgac
     4321 ctgctcgaac tgtactgcgg caacggcaac ttcacggtcg ccctggcgcc gctgttccgc
     4381 cgcgtgctcg ccaccgaagt gagcaagacc tcggtcaagg ccgcgctgca caacctggag
     4441 cgcaacgcca tcgccaacac ggcggtcgtc cgcatggcga gcgaggaact gagcgccgcg
     4501 ctggccggcc gcgaaagctt ccagcgcctg cgcgagatcg acctcgccag ccacgacttc
     4561 agcaccctgt tcgtcgatcc gccgcgcagc ggcctcgacc cggcgacgct ggaaatcgcc
     4621 gccggcttcg ccaacatcct ctacatctcg tgcaatccgc agaccctgcg cgacaacgtc
     4681 gccgccctgc aggccacgca ccgcatcgcc gcggcggcgg tcttcgacca gttcccctac
     4741 acccaccacc tggagtgcgg ggtgctgctg cagcgccgct gagcggccgc cttcagcgga
     4801 accagccggg cagcagcgag gccgtcggca gaaacagctt ctcgacggcg gcgatgtagc
     4861 gcttgcgggt gcagaagacg acgacgtggt cgccggcttc gatcagtgtg tcgtggtgcg
     4921 ggatgatcac ccgcccgccg cgcacgacgg cggcgatgct ggcgccgtgg atttccggca
     4981 gttcctcgat ccggcggccg accacacgcg aactgccgcg ctcgccgcgc accaccagct
     5041 cgagcgcttc ggcagcgccg cggcgcaggc tgtgcacgcg cgccacgtcg gcctggcgga
     5101 cgtgcgtcag gaggccgccg atcgacacct gggccggcga caggccgatg tcgatcggtc
     5161 cgccctggac catgtcggcg taggcctcgc ggttgatcag ggcgagcacg cgacgacagc
     5221 cgaggcgctt ggccagcgag gcggccatga tgttgttttc gtcgtcgttg gtcagcgcca
     5281 ggaacatgtc catgctgccg atctcctcgc gcgccagcag cgtctcgtcg gtggcctcgc
     5341 cggtcagcac cagggtccgg ttgagccagc cggcgaccag cgcggcgcgc gccggccggc
     5401 tttcgaccag cttgacttcg cagcttcctt ccaggcggcc ggcgacgcgc gcgccgacgt
     5461 tgccggcacc ggcgatcatc accttgcgca ccggcttctg catgcgccgc aactccttga
     5521 gcacggtccg gatatgctcg gtggcggcaa gcaggaagac ctcgtcgccg gactggacga
     5581 cggtgtcgcc gtcgatgttg atcgcccgcg tgtcgcggaa gatcgccgcg atccgggcat
     5641 cgacgccgga cggcaggtgg ccgcgcatgg cgcggatcgg cttgccgacc agcaggccgc
     5701 cctcgtaggc gcgcaagccg accatggtca gccgcccttc ggcgaactcg accacctgca
     5761 gggcttccgg gtattcaacc agcttgccga tgtagtcgct gatttcctgc tccggacaga
     5821 gcgcgaaatc gaccgcgaag ttgtcggtcg acaacagcgt cgggtcgagg aaatcgggcg
     5881 aacgcaggcg agcgatccgg gtcggcacgg cgaagcggct gtgcgccagc ttgcaggcga
     5941 ccaggttgga ctggtcggac tgggtgacgg cgacgatcag gtcggcggtt tcgacgccgg
     6001 cttcggcgag caccgacggg aaggcggcgt tgccggtcac cgtgcgcacg tcgagacggt
     6061 cggccagttg cgccaacgcc ccggcatcga gatcgaccaa ggtgatgtcg ttggcttccg
     6121 agaccagcgc ttcggcgaga ctggcgccga cctggccggc accgaggatg aggatgttca
     6181 tgcggtattg tcttcgttca acgtcgccag aaagccggcg tcaggaccac gagcagggtg
     6241 aagatttcca gacgaccgag cagcatcgcg aaaatgcaga cccaggtctg aaagtcctcg
     6301 agcacggcga atgtcgtcga tggaccgacc tcgccaagcc ccggcccggt attgttgacc
     6361 gaagcgacca ccgccgtgaa ggcggtgatc acgtcgagcc cggtgaatac catgaccagg
     6421 gtcagcgaga cgatgctgac catgtacatg aaaccgaagg cgagcaccgc gaacaggatg
     6481 ctcgacggcg ccacctggcc gccgatgcgc acgctgtaga cggcgttcgg gtgcaaggca
     6541 cggacgatct cgcggtagac ctgcttgtag agcagcaggg cgcgcgccat cttgatgccg
     6601 ccgccggtgg acccggcgct ggtcgcaaag ctgcacagga acagcatcca gagcggcgcg
     6661 aacaacggcc actgggcgaa gtccacgctc gcgtaaccgg tggtcgtggc gatcgagatg
     6721 acgttgaacg ccgagtgccg cagcgcttcc gcaaaatccg ggtagatctc gttggcgcgc
     6781 aggaagagcg cgatgccgag caccgagacc aggcacacgc cgagaaacca gccggcttcc
     6841 ggatcgttca ggtagggttt gagtgagccg cggctgacgg cgacgaacag ggtgccgaaa
     6901 ttcatgccgg cgatcagcat gaagacgatc gccaccgcct cgatcgccgg cgaattgaaa
     6961 tagccgtaac tcgcatcgtg tgtcgagaaa ccgcccagac ccatcgtcga aaaggcgtgg
     7021 cagacggcgt cgaaccagcc catgccggcg acccgatagc cgagcacgca aaggatggtg
     7081 acgccgacat agacgaccca caaacccttg gccgtgctgg cgatgcgcgg cgtcatcttg
     7141 gcgtccttca tcggccccgg cgtctcggcc ttgaacatct ggcgcccgcc gatgccgagc
     7201 agcggcagga tggcgatggc gagaacgatc agccccatgc cgccgaccca caccagctcg
     7261 tgacgccaca ggttgatcga cagcggtagc tggtcgaggc cggaaagcac ggtggcaccg
     7321 gtcgtcgtca ggccggacat cgcctcgaaa taggcgtcgg tatggctgat gcccagttgc
     7381 agcatgaagg gcagcgcggc aaaggccggc aacacggtcc acaccagcac caccatcagg
     7441 aagccgtcac gcacgttcat ttcgcgcttg acgttgcggt agcggtacca gagatagaga
     7501 ccgcagagca gggtcagcgc gaaaacctcg tcgtagacgg cctgcgcgcc gtcgtccgcg
     7561 gcccaggaaa ggaacagcgg cggcagcagg gtcagcgcga agagcatgat gaccatgccc
     7621 agcgcccgca ggacgggagc gtaacggttc atgcgtcggg ctggaagcgg taaccgacgc
     7681 cggtttcggt caggaaatga cgcggctggg ttgggtcgtc ctcgagtttc tggcgcagat
     7741 gaccaacata gacgcgcagg tagtgactgc tctcgacgta ggaggcgccc cagatttcgg
     7801 ccagcaactt gcgctgcgtc agcacctggc cggggtgggc gagcaggtag acgaagaacc
     7861 gcaggacatt ctcgggct
//
LOCUS       DAOSDX010000013         7794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3448_length_7794_cov_2.860059, whole genome shotgun sequence.
ACCESSION   DAOSDX010000013 DAOSDX010000000
VERSION     DAOSDX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3448_length_7794_cov_2.860059"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLN02_00575"
     CDS             complement(<1..275)
                     /locus_tag="PLN02_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOI50845.1"
                     /translation="MSTRLPGPLMIDIAGTELTDLDRERLCHPLVGGIILFTRNYRDR
                     AQLTALCDEIHALREPRLLIAVDHEGGRVQRFREGFTRLPPMRALGQ"
     gene            complement(272..649)
                     /gene="acpS"
                     /locus_tag="PLN02_00580"
     CDS             complement(272..649)
                     /gene="acpS"
                     /locus_tag="PLN02_00580"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOI50846.1"
                     /translation="MIFGIGTDIVAVARLRGMWERHGDKALDKLLAPDELDDFARAAD
                     KGRFLAKRFAAKEAFAKAFGSGVRPPVLLPAIAVGHDELGKPVFNFHGQLAEIVESRR
                     LIAHLSISDEAEYAVAYVLLEQA"
     gene            complement(646..1407)
                     /locus_tag="PLN02_00585"
     CDS             complement(646..1407)
                     /locus_tag="PLN02_00585"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOI50847.1"
                     /translation="MIELGVNIDHIATVRQARRTYEPDPVWGAVEAHLGGADGITVHL
                     REDRRHIQDSDVRRLKETTQIKLNLEMAATDEMVGIACATRPEMAMLVPEGRHEVTTE
                     GGLDIVGQEARLKDVIARLRDAGITTSVFIDAEIPQIEAAARIGASVCEIHTGPYAHA
                     FYEKGRDAEAPAVLAELQKIRVAGRAIRQLGMRFNAGHALNYYNIQPVARLAGIRELH
                     IGHAIVSRAVFVGLREAVREMKALMVAAAERPEAE"
     gene            complement(1486..1800)
                     /locus_tag="PLN02_00590"
     CDS             complement(1486..1800)
                     /locus_tag="PLN02_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50848.1"
                     /translation="MTKRNLFAEIAEGFDALASERAGKQTLRTHKVEVQPLPALTAEE
                     LVSLRERLHLSRPVFARYLRTNPRTLENWEQGRAKPNAQATLLIRLVEKFPDTVDRLA
                     AV"
     gene            complement(1797..2135)
                     /locus_tag="PLN02_00595"
     CDS             complement(1797..2135)
                     /locus_tag="PLN02_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOI50849.1"
                     /translation="MKALFVELPAFSRYRADYLDDEGFRGLQQFMLTNPEAGDVIEGT
                     GGLRKLRHGDPRRGKGKRGGLRVIYYWWAGGRQFWLFTLYDKDESSDLSADEKKVLKQ
                     MLKAELEARR"
     gene            complement(2179..2907)
                     /gene="recO"
                     /locus_tag="PLN02_00600"
     CDS             complement(2179..2907)
                     /gene="recO"
                     /locus_tag="PLN02_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOI50850.1"
                     /translation="MSLRSKVDGQPAYVLHSMPFRETSLIVEVFSRDFGRMALLARGA
                     RRPRAAIRGLLMGFQPLEIGWAGKGEVLTLMKAEWVGGQPLLGGEALFCGYYLNELLM
                     HLLPREDAHQALFARYAETLQRLAAHNDVATREGDLRSFEKALLQELGYGLMLEHDAA
                     GEPIVAEAFYTYRMEQGPVRLAHAEADAQVVRGSSLLDLGAGDFSNPRSRGEAKTLMR
                     TLLSYYLGGQELETRKIFKELQEL"
     gene            complement(2920..3804)
                     /gene="era"
                     /locus_tag="PLN02_00605"
     CDS             complement(2920..3804)
                     /gene="era"
                     /locus_tag="PLN02_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOI50851.1"
                     /translation="MTEIRSGYIAIVGRPNVGKSTLLNHLIGEKISIVSRKAQTTRHR
                     ITGILTEADAQFVFVDTPGFQTKFSNALNRAMNRGVTQTLNDVDLVLFVIEAGRYDAK
                     DKAVVRLLPKDRPVILVINKTDLLKNKEALLPFLAEVSADFDYTAVVPVSAAKGRQTQ
                     DLLKEARKHLPNEGLMFPEDDLTDKSERFLAAEYIREKVFRLLGDELPYATAVEIEKF
                     EVEGNLRRIYAAIVVDRENHKAIVIGKGGESLKRIASEARQDMERLFDGKVYLEVWVK
                     VKSGWNDDERLLKSLGYE"
     gene            complement(3801..4469)
                     /gene="rnc"
                     /locus_tag="PLN02_00610"
     CDS             complement(3801..4469)
                     /gene="rnc"
                     /locus_tag="PLN02_00610"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOI50852.1"
                     /translation="MSLQILAERIGHRFADPALLRTALTHRSFGTPNNERLEFLGDGI
                     LDCVIAALLYHRFPTLPEGDLSRLRANLVRQESLHRLAQELEIGKALRLGEGELKSGG
                     ATRPSILADALEAIFGAIYLDAGFDTASSAISRLYAPMLGELKPGQFQKDAKTRLQEW
                     LQGRKKPLPRYVLQETSGAAHEQSFVVACEIDQPALRTRGTGSSRRIAEQNAAEAALK
                     ALNP"
     gene            complement(4472..4825)
                     /locus_tag="PLN02_00615"
     CDS             complement(4472..4825)
                     /locus_tag="PLN02_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HOI50853.1"
                     /translation="MKQQRGVALTGLILWGIVLILVAVLGMKVVPSVIEYYKIQSAIK
                     AVVEKAKPEATVPELRKSFEKFAEVDQLAFPSKNLDIFKNNRGQIVIEFAYDKKIPLF
                     ANASLLIEYKGTTAN"
     gene            complement(4836..5624)
                     /gene="lepB"
                     /locus_tag="PLN02_00620"
     CDS             complement(4836..5624)
                     /gene="lepB"
                     /locus_tag="PLN02_00620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOI50854.1"
                     /translation="MNFALILLVLTVVTGILYAIDILKFRKLRAANDKQPLWVEWGAD
                     FFPVILIVFVLRSFLFEPFKIPSGSMIPTLLVGDYILVNKYAYGIRLPVINTKVIEVG
                     DPQRGDVMVFRYPEDPSLDYIKRVVGLPGDTIDYHNKQLRINGEPVAMKPEADFLHAE
                     RLYYSQQFTEKLGQVEHRVLNDADAPAFIPGVSSFPYRENCTYNASGVTCKVPDGHYF
                     MMGDNRDNSRDSRAWGFVPEENIVGKAFFIWLNFSDFSRIGSFR"
     gene            complement(5633..7423)
                     /gene="lepA"
                     /locus_tag="PLN02_00625"
     CDS             complement(5633..7423)
                     /gene="lepA"
                     /locus_tag="PLN02_00625"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOI50855.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRIIHLCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALQYKARDGRVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELDVEVLPVLNKIDLPSADPDTARQEIEDVIGIDATDAVL
                     ASAKTGLGVEDILEAVVARVPAPQGDPEAPLKALIIDSWFDNYVGVVMLVRVVDGEMK
                     PKDKLYFMATGATQLCEQVGVFTPKSLQRPSLKAGEVGFVISGIKELKAAKVGDTITL
                     ADRKAAEPLPGFKEIKPQVFAGLYPVESNQYDSLREALEKLKLNDASLQYEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPTVVYEVVQRDGSVIQVENPAKLPE
                     ISKIEEVREPIITATIFVPQDFLGNVITLCNQKRGNQVDMHYHGRQVKLVYEMPMAEV
                     VMDFFDKLKSCSKGYASLDYDFKEYRAADVVKLDILINGEKVDALSLIVHRSNSQYRG
                     RELASKMRELIPRQMYDVAIQAAIGSHIIARENVKAMRKDVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAVLRVDN"
     gene            complement(7482..7727)
                     /locus_tag="PLN02_00630"
     CDS             complement(7482..7727)
                     /locus_tag="PLN02_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOI50856.1"
                     /translation="MAIELTLLSRSYCHLCHDMEEALAPLLAEFEASLQVLDVDADPD
                     LEARYDELVPVLLHGETELCHHFLDAAKTREYLSGIR"
BASE COUNT         1372 a         2382 c         2489 g         1551 t
ORIGIN      
        1 aactggccga gggcgcgcat cggcggcagc cgggtgaagc cctcgcggaa gcgctgcacc
       61 ctgcccccct cgtgatcgac ggcgatcagc aggcgcggct cgcgcagcgc gtggatttcg
      121 tcgcacagcg ccgtcagttg ggcgcggtcg cggtagttgc gcgtgaacag gatgatgccg
      181 ccgaccagcg gatggcacag gcgttcgcgg tcgaggtcgg tgagttcggt gccggcgatg
      241 tcgatcatca gcggaccggg cagtctggtg ctcatgcttg ctccagcaaa acgtaggcga
      301 cggcgtattc ggcttcgtcg ctgatcgaga ggtgcgcgat caggcggcgg ctttcgacga
      361 tttccgccag ttgaccgtga aagttgaata ccggcttgcc cagttcgtcg tggccgacgg
      421 caatcgccgg cagcagcacc ggcggccgca cgccgctgcc gaaggccttg gcgaaggctt
      481 ccttggcggc aaagcgcttg gccaggaagc ggcccttgtc ggcggcgcgt gcgaaatcgt
      541 ccagctcgtc cggcgccagc agcttgtcca gcgccttgtc gccgtgccgt tcccacatgc
      601 cgcgcagccg ggcgacggcg acgatgtcgg tgccgatgcc gaagatcatt cggcttccgg
      661 acgctcggcg gcggctacca tcagcgcctt catctcgcgc accgcctcgc gcaggccgac
      721 aaagacggcg cggctgacga tggcgtggcc gatgtgcagc tcgcggatgc cggccagccg
      781 ggcgaccggc tggatgttgt agtagttgag cgcatggccg gcgttgaagc gcatgccgag
      841 ctggcggatg gcacggccgg cgacgcggat tttctgtagt tcagcgagca ccgccggcgc
      901 ctcggcatcg cggcctttct cgtagaaggc gtgggcgtag gggccggtgt ggatctcgca
      961 gacgctggcg ccgatccgcg ccgccgcctc gatctgcggg atttcggcgt cgatgaagac
     1021 gctggtggtg atgccggcgt cgcgcaggcg ggcgatcacg tccttgagcc gggcttcctg
     1081 gccgacgatg tcgaggccgc cctcggtggt cacctcgtgc cgcccttccg gcaccagcat
     1141 ggccatttcc ggccgggtcg cgcaggcgat gccgaccatc tcgtcggtcg ccgccatttc
     1201 caggttgagc ttgatctgcg tcgtttcctt gagccggcgg acatcagagt cctgaatgtg
     1261 ccggcgatcc tcgcgcagat gcacggtgat gccgtcggcg ccgcccagat gcgcctcgac
     1321 ggcgccccag acgggatcgg gttcgtaggt gcgccgggcc tggcggacgg tggcgatgtg
     1381 gtcgatgttg acgccgagtt cgatcatggt tcctgcaact ctctgatgaa tgccgctcac
     1441 gacgggcagc ctgtggattg acgtttactg ctgagatttc gggggtcaaa ctgcggcaag
     1501 ccgatccacc gtatccggaa atttttccac caggcgaatc agtaatgtcg cctgggcatt
     1561 tggcttggct ctgccctgct cccagttttc cagcgtcctc ggattcgtac gcaggtagcg
     1621 agcgaatacc ggacgcgaca gatgcagccg ttcgcgcaaa ctgaccagtt cctcagcagt
     1681 cagcgccggc aacggctgaa cttcgacctt atgcgtacgc aaggtctgct tgccggcacg
     1741 ctcgctcgcc agcgcatcaa aaccctcggc aatttccgca aacagattcc gcttggtcat
     1801 cttcgtgcct ccaattcggc tttcaacatc tgcttgagca ccttcttctc gtcggcactc
     1861 aaatcactcg attcgtcctt gtcgtacagg gtgaacagcc agaactgccg gcccccagcc
     1921 caccagtagt agatcacccg caagccgccg cgcttgcctt tgccgcgacg agggtcgcca
     1981 tggcgcaact tgcgcagacc gccagtccct tcgatcacat cgccagcctc cgggttcgtc
     2041 agcatgaatt gctgcaaacc acgaaagcct tcgtcatcga gatagtcggc tctgtacctc
     2101 gaaaatgcgg ggagttcaac gaacaaggct ttcatgaaat atacgcaagt tacgtttata
     2161 tagcaagttc gagacggact acagttcctg caactccttg aaaatcttgc gggtttccag
     2221 ttcctggccg cccaggtaat agctcagcag cgtgcgcatc agcgtcttgg cctcgccgcg
     2281 cgaacgcggg ttggagaaat cgccggcgcc gagatcgagc agactgctgc cgcgcaccac
     2341 ctgggcgtcg gcttcggcgt gcgccaggcg caccggcccc tgctccatcc ggtaagtgta
     2401 gaacgcctcg gcgacaatcg gctcgccggc ggcgtcatgc tcgagcatca ggccgtagcc
     2461 gagctcctgc agcaacgctt tctcgaagct gcgcaggtcg ccctcgcggg ttgccacgtc
     2521 gttgtgcgcg gcgagccgct gcagggtttc ggcgtagcgg gcaaacagcg cctggtgggc
     2581 gtcctcgcgc ggcagcaggt gcatcagcag ttcgttcagg tagtaaccgc agaacagcgc
     2641 ctcgccgccg agcagcggct ggccgccgac ccattcggcc ttcatcagcg tcagcacctc
     2701 gcccttgccg gcccagccga tttccagcgg ctggaagccc atcagcaggc cgcggatcgc
     2761 cgcccgcggc cggcgggcgc cgcgcgccaa aagcgccatg cggccgaaat cgcggctgaa
     2821 gacctcgacg atcaggctgg tctcgcgaaa cggcatgctg tgcagcacgt aggcgggctg
     2881 gccgtcgacc ttgctgcgca gactcatggc gaaacaggct tactcgtagc cgaggctctt
     2941 gagcaggcgt tcgtcgtcgt tccagcccga cttcaccttg acccagactt cgagatagac
     3001 cttgccgtcg aacagccgct ccatgtcctg gcgcgcctcg ctggcgatgc gcttcaggga
     3061 ttcgccgccc ttgccgatga cgatcgcctt gtgattctcg cggtcgacga cgatggctgc
     3121 gtaaatccgg cgcaggttgc cctcgacttc gaatttctcg atctcgacag cagtggcgta
     3181 aggcagttcg tcgccgagca ggcggaagac cttctcgcgg atgtattcgg cggcgaggaa
     3241 acgctccgac ttgtcggtca ggtcgtcttc cgggaacatc aggccttcgt tcggcaggtg
     3301 cttgcgcgcc tccttgagca ggtcctgcgt ctgccggccc ttggcggcgc tgaccgggac
     3361 cacggcggtg tagtcgaaat cggccgaaac ctcggcgagg aaaggcagca gcgcttcctt
     3421 gttcttcagc aggtcggtct tgttgatgac caggatcacc gggcggtctt tcggcaacag
     3481 gcgcaccacc gccttgtcct tggcgtcgta gcgaccggcc tcgatgacga agaggacgag
     3541 atcgacgtcg ttcaaggtct gcgtcacgcc gcgattcatc gcccgattca gggcgttcga
     3601 gaacttggtc tggaagcccg gcgtatcgac gaagacgaac tgcgcgtcgg cctcggtcag
     3661 gatgcctgtg atgcggtgcc gggtggtctg cgccttgcgc gaaacgatgc tgatcttctc
     3721 gccgatcagg tggttgagca aggtcgactt gccgacgttg ggtcggccga cgatggcgat
     3781 gtagccggag cggatttcgg tcatgggttc agggctttca aggccgcttc ggcggcgttc
     3841 tgttcggcga tgcggcggct gctaccggta ccgcgggtac gcagcgccgg ctgatcgatc
     3901 tcgcaggcaa cgacgaagga ttgttcatgg gcggcgccac tggtttcctg cagcacatag
     3961 cgcggcagcg gcttcttacg gccctgcagc cattcctgca ggcgggtctt ggcgtctttc
     4021 tggaattgcc ccggcttgag ttcgccaagc attggcgcgt aaaggcggga aatcgcactg
     4081 ctcgcggtgt cgaaaccggc atcgaggtaa atcgcgccga agatcgcttc cagcgcatca
     4141 gcgaggatcg aggggcgtgt cgcgccgccg cttttcagct cgccttcgcc cagacgcagg
     4201 gccttgccga tttccagctc ctgcgccagc cggtgcaggc tttcctggcg caccagattg
     4261 gcacgcaggc gcgacaagtc gccttcaggc aaggtcggaa agcgatggta gaggagcgcg
     4321 gcgatgacac aatcgagaat gccgtcgccg aggaattcca gccgttcgtt attgggcgta
     4381 ccgaaactgc gatgggtcag cgccgtccgc aacagtgccg gatcggcaaa acgatggccg
     4441 atccgctcgg caaggatctg caagctcatg cctagttggc ggtcgtcccc ttgtattcga
     4501 tcagcaggct agcgttggcg aacaagggaa ttttcttgtc gtaggcgaat tcgatgacga
     4561 tctgcccgcg gttgttcttg aaaatgtcga gattcttcga gggaaatgcc aactgatcga
     4621 cttcggcaaa cttttcgaag gatttgcgca actcggggac cgtcgcttcc ggcttggcct
     4681 tctcgaccac cgccttgatc gcgctctgaa tcttgtaata ctcgatcacc gaaggcacca
     4741 ccttcatgcc cagaacggcc acgaggatga ggacgatacc ccagaggatc agcccggtca
     4801 gtgccacgcc gcgttgttgt ttcattactc tcaccttacc tgaatgaacc gatacggctg
     4861 aaatcgctga aattcagcca gatgaaaaat gccttgccga cgatgttctc ttccggcaca
     4921 aacccccagg cacggctatc gcggctgttg tcgcgattgt cgcccatcat gaagtaatga
     4981 ccgtccggca ccttgcaagt cacgccactg gcattgtaag tgcaattctc gcggtaggga
     5041 aaggacgata cgccagggat gaaggccggt gcatcggcat cgttgaggac gcgatgttcg
     5101 acttggccga gcttttccgt gaactgctgc gaatagtaca ggcgctcggc gtgcaggaaa
     5161 tctgcctccg gcttcatcgc caccggctcg ccgttgatgc gcagttgctt gttgtggtaa
     5221 tcgatggtgt cgcccggcag gccgacgacg cgcttgatgt agtcgagcga cggatcctcg
     5281 gggtagcgga agaccatgac gtcgccgcgc tgcggatcgc cgacttcgat caccttggtg
     5341 ttgatcaccg gcaggcggat gccgtaggcg tacttgttga ccaggatgta gtcgccgacc
     5401 agcaaggtcg gaatcatcga gccggatgga atcttgaacg gctcgaacag gaaggagcga
     5461 agcacgaaga cgatcaggat caccgggaag aaatcggcgc cccactccac ccacagcggc
     5521 tgtttgtcgt tggcggcgcg cagcttgcgg aacttgagga tgtcaatggc gtagaggatg
     5581 ccggtaacga cagtcaggac gaggaggatc agggcgaagt tcatgcgtta tctcagttat
     5641 cgacgcgcag cacggcgagg aaggcttcct gcgggatttc caccgagccg acctgcttca
     5701 tgcgcttctt gccggctttc tgcttttcca gcagcttctt cttgcgggtg atgtcgccgc
     5761 cgtagcactt ggccagcacg tccttgcgca tcgccttgac gttttcgcgg gcgatgatgt
     5821 gcgagccgat ggccgcctgg atggcgacgt cgtacatctg gcggggaatc agctcgcgca
     5881 tcttggacgc gagttcgcgg ccgcggtact gcgagttcga gcggtgcacg atcagcgaca
     5941 acgcatcgac cttctcgcca ttgatcagga tgtcgagctt gaccacgtcg gcggcgcggt
     6001 attccttgaa atcgtagtcg agcgaagcgt agcccttgga gcaggatttc agcttgtcga
     6061 agaaatccat gaccacttcg gccatcggca tttcataaac gagcttcacc tggcgaccgt
     6121 ggtagtgcat gtcgacctgg ttgccgcgct tctggttgca cagcgtgatg acgttgccga
     6181 gaaaatcctg cggcacgaag atggtggcgg tgatgatcgg ctcgcgcact tcctcgatct
     6241 tggaaatttc cggcagcttg gccggatttt cgacctggat caccgagccg tcgcgctgga
     6301 cgacctcgta caccacggtc ggcgcggtgg tgatcaggtc ctggtcgaac tcgcgctcca
     6361 ggcgttcctg gacgatttcc atgtgcagca ggccaaggaa gccgcagcgg aagccgaagc
     6421 cgagcgcttg cgagacttcc ggttcgtact gcagcgaggc gtcgttgagc ttgagcttct
     6481 ccagcgcttc gcgcagcgag tcgtactggt tcgattcaac cggatagagg ccggcgaaca
     6541 cctgcggctt gatttccttg aagccgggca agggctcggc cgccttgcgg tcggccaggg
     6601 tgatcgtgtc gccaaccttg gcggctttca gttccttgat cccggagatc acgaagccga
     6661 cttcgccggc cttcagcgac ggacgctgca gcgatttcgg ggtgaacacg ccgacttgtt
     6721 cgcacaactg agtggcgccg gtggccatga aatagagctt gtccttcggc ttcatctcgc
     6781 cgtcgacgac gcgcaccagc atgacgacgc cgacgtagtt gtcgaaccat gagtcgatga
     6841 tcagcgcctt cagcggcgcc tccggatcac cttggggcgc cggcacacgg gcgaccaccg
     6901 cttccaggat atcctcgacg ccgaggccgg tcttggccga ggcgagcacc gcgtcggtgg
     6961 cgtcgatgcc gatcacgtcc tcgatttcct gacgggcggt atcgggatct gccgacggca
     7021 ggtcgatctt gttcagcacc ggcagcacct cgacgtcgag ctcaatcgcc gtgtagcagt
     7081 tggccaccgt ctgcgcctcg acaccctgcg aggcatcgac gaccagcagc gcaccttcgc
     7141 aggcggacag cgagcgcgag acttcgtagg agaagtcgac gtgtcccggc gtgtcgatca
     7201 ggttcaggtt gtacaccctg ccgtcgcgcg ccttgtactg cagcgctgcc gtctgtgcct
     7261 tgatggtgat gccgcgttcg cgctcgatat ccatcgaatc gagcacctgc gcctccattt
     7321 cgcggtcgga aagaccgccg cagagatgga tgatgcgatc ggccagggtc gatttgccgt
     7381 ggtcgatgtg ggcgatgatc gaaaaattgc ggatatggtc cattgcagcc agtaaaaaag
     7441 ggcgcgctcg gcgccctgct cggttcgaaa gaccgtgaat tctagcggat tccgctcaaa
     7501 tattcacggg ttttggcggc gtccaggaag tggtggcata gctcggtttc cccgtgcagc
     7561 aggaccggca ccagttcgtc gtaacgggct tcaagatcgg ggtcggcatc gacgtcaagc
     7621 acctggaggc tggcctcgaa ctcggcgagc agcggcgcca acgcctcctc catgtcatgg
     7681 cagaggtggc agtagctgcg gctgagcagg gtcagttcaa tggccattgc cgccccggat
     7741 ggtgacgaaa ttctgcaggt tgccgcgccg gatcaacagc gtaacggcgc tgcc
//
LOCUS       DAOSDX010000014         7764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3465_length_7764_cov_2.983526, whole genome shotgun sequence.
ACCESSION   DAOSDX010000014 DAOSDX010000000
VERSION     DAOSDX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3465_length_7764_cov_2.983526"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="PLN02_00635"
     CDS             complement(<1..123)
                     /locus_tag="PLN02_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOI50857.1"
                     /translation="MRQYDVLIIGSGLAGLTLALKVASDKKVCLVSKRGINDSSS"
     gene            complement(380..2080)
                     /gene="arsA"
                     /locus_tag="PLN02_00640"
     CDS             complement(380..2080)
                     /gene="arsA"
                     /locus_tag="PLN02_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HOI50858.1"
                     /translation="MLDLDQAPRYLFFTGKGGVGKTSLSCASGLALAETGKRVLIVST
                     DPASNLDEVLETPLAGTPTPVRGVPGLSALNIDPEAAAAAYRERMVAPYRGILPAAAI
                     QSMEEQFSGACTVEIAAFDEFAKLLGEPASTADFDHVIFDTAPTGHTLRLLTLPSAWS
                     GYIDSSHGGASCLGPLAGLEKQKALYAATVERLSDPARTRVVLVARADAAALREAERT
                     RHELAELGVANLQLAINGLFYAAGNDPVALAMEQRSTAALLAMPGGLGALPRRVVPFL
                     PRGTVGLAALRAIVAPAPAAAPEVAPATSTKLPGGLAALIDELASAGRGVVMTMGKGG
                     VGKTTVAAAVAVALARRGHRVHLTTTDPAAHVAAAVDGEIAGLSLSRIDPTAEVAAYT
                     EEVLSKARGALDDSALALLEEDLRSPCTEEIAVFRAFAREVDRGNDGFVVLDTAPTGH
                     TILLLDAAEAYHREVLRTRGEMPAAVTQLLPRLRDPGFTHVLIVTLPEATPVHEAERL
                     QGDLARAGIQPWAWVIEQSLLASGSRDPLLALRGAYEIPYIRRVTDSLSPRCARSTSS
                     "
     gene            complement(2090..2452)
                     /gene="arsD"
                     /locus_tag="PLN02_00645"
     CDS             complement(2090..2452)
                     /gene="arsD"
                     /locus_tag="PLN02_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HOI50859.1"
                     /translation="MQKLEVYDPAMCCPTGVCGVDVDPVLVQFAADLAWVGEQGVAVQ
                     RYNLGQEPQAFAANPAVVKEMEAGIDRLPIVVIDGKIVSSGLYPTRAQLAQKLGLTLG
                     SSEEAPKKSCCSPKSGCC"
     gene            complement(2468..3538)
                     /gene="arsB"
                     /locus_tag="PLN02_00650"
     CDS             complement(2468..3538)
                     /gene="arsB"
                     /locus_tag="PLN02_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOI50860.1"
                     /translation="MSAQCGVAAKQAAGAPMGFFERWLSLWVVLCIVAGVLLGQWFPG
                     AFQALGGMEVAQVNLPVGLLIWVMIVPMLMKIDFATIHEVKDQKAGIGITLFVNWAIK
                     PFTMAALGWLFIRYVFAPYLPAEQLDSYIAGLILLGAAPCTAMVFVWSNLCRGNANFT
                     ISQVALNDLVMVFAFAPIVALLLGVSAIPVPWDTLLLSVVMYIVIPLAIAQGLRKHFL
                     RGGEAAFKRAIERIGPFTIVALLATLVLLFAFQGEAIVRQPLIIAMLAVPILLQGLLI
                     AAIGYWLCHKFRVQHDVAGPATMIGASNFFELAVAVAIVLYGFDSGAALATVVGVLIE
                     VPVMLWLVKMVNSTKTWYEKSL"
     gene            complement(3535..4023)
                     /locus_tag="PLN02_00655"
     CDS             complement(3535..4023)
                     /locus_tag="PLN02_00655"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOI50861.1"
                     /translation="MKTVLVLCTGNSARSILGEALFNHLGGGRIRAFSAGSQPTGTVN
                     PVALETLASHGVPQPEARSKSWDEFAAPGAPAIDYIFTVCASAAGETCPIWPGHPTTA
                     HWGIADPAHVEPLAARQAAFERAYQVLRQRVEAFLQLPLETMSAAEIKMAAQRIHQEC
                     EQ"
     gene            complement(4034..4534)
                     /locus_tag="PLN02_00660"
     CDS             complement(4034..4534)
                     /locus_tag="PLN02_00660"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOI50862.1"
                     /translation="MNDRIYNVLFLCTGNSARSILAEALLNHLGKERFRAYSAGSRPT
                     GTVNPYARELLERQHLPTAQLRSKSWDEFASADAPRLDFIFTVCDNAAGEVCPVWPGQ
                     PMTAHWGVEDPAAVGGDDEARREAVMKASIQLKRRIELFLALPIDKLDAMALQDKLDG
                     IGREKS"
     gene            complement(4703..4897)
                     /locus_tag="PLN02_00665"
                     /pseudo
     CDS             complement(4703..4897)
                     /locus_tag="PLN02_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5050..6087
                     /gene="pstS"
                     /locus_tag="PLN02_00670"
     CDS             5050..6087
                     /gene="pstS"
                     /locus_tag="PLN02_00670"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOI50863.1"
                     /translation="MLQSRIFRASLLALSLGLAGFGAQAAEITGAGTSFPAPVYAKWA
                     DAYQKATGNKVNYQSIGSGGGIKQINAKTVDFGASDMPLKQEVLDKDGLVQFPTVIGG
                     VVPVVNVVGVKAGDLRLTGEIVADIYLGKITKWNDKAIAELNPNVKLPGDDIAVVRRA
                     DGSGTTFIFTNYLSKASSDWKSKVGEGTAVQWPTGMGGKGNEGVAAFLQRIPNSIGYV
                     EYAYAKQNHLAWTWLKNPAGEFVRPDDDNFKAAAAGADWSKSDFGEILTAQSGKGAWP
                     ISGATFILMHKVQDKPAQAAEVLKFFEWSYKNGAKLASDLDYVPLPDSLVKQIAASWA
                     NIKDASGKAVK"
     gene            6196..7149
                     /gene="pstC"
                     /locus_tag="PLN02_00675"
     CDS             6196..7149
                     /gene="pstC"
                     /locus_tag="PLN02_00675"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HOI50864.1"
                     /translation="MSETQAKGRLADLLFFNLTRTVAFITLALLGGIVISLTWSALPS
                     IEKFGFSFLTSSAWNPPADDFGALVPIYGTIITSIIALVIALPISFGIALFLTEMAPT
                     WLRRPLGIAIELLAGIPSIVYGIWGLLVFAPIFGQYVQPFIAQTLGKLPLIGALFSGP
                     SLGIGILAAGIILAIMIIPFIASVMRDVFELVPPMLKESCYGLGGTTWETVSKVVLPY
                     TRVGVIGGVMLGLGRALGETMAVTFVIGNASLLGSFSLFMPGNSITSALANEFAEATP
                     GLYTAALVELGLILFVITFIVLSLSKLLLLSMEKQNGGGAH"
     gene            7160..>7764
                     /gene="pstA"
                     /locus_tag="PLN02_00680"
     CDS             7160..>7764
                     /gene="pstA"
                     /locus_tag="PLN02_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOI50865.1"
                     /translation="MDLRTRRKTTNVIALGLSLAAMAFGLFWLIWILWMVFEQGIGAL
                     SLDLLTLDTPAPGNAGGLRNAIFGSLVMVGLAMLIATPVAILAGIYLAEYGRRTVLGQ
                     VTRFVNDILLSAPSIVIGLFIYAIYVAQVGKFSGMSGALALALLVIPVVVRTTEDMLI
                     LVPNALREAAFALGAPKWKVILMVTLRSAKTGVITGILLALA"
BASE COUNT         1352 a         2451 c         2576 g         1385 t
ORIGIN      
        1 gctcgagctg tcgttgatgc cacgtttact gaccaggcag actttcttgt cggacgctac
       61 cttgagcgcg agcgtgaggc cggcgagacc gctgccgatg atgagtacgt cgtattggcg
      121 catggtgtct ggtgtgcttg gggtaatgga cgggatgaac taatgcgagt ttagcgctgt
      181 ctgttaggac aagctagcaa ttgattcgta tcaagcctgg gagagagtga atgttaaaca
      241 cgaagaatct gcaagaattt tcacagctga gcttcgttga tacagcgatg caagggtata
      301 ctccgacagt tgaaaaagta ctgacaagga gcaaggcctc tatgggttcc aaccccacag
      361 cgggcaaccg tgaaatcgat caggaactgg ttgagcgcgc gcaacgcggc gacaagctgt
      421 cggtgacgcg ccggatgtaa gggatttcgt aagcgccgcg cagggcgagc agcgggtcgc
      481 ggctgccact ggccagcagg ctctgctcga tgacccaggc ccagggctgg atgccggccc
      541 gggcgaggtc gccttgcagg cgctcggctt cgtgtaccgg cgtcgcttcc ggcagggtga
      601 cgatcagcac gtgggtgaag cccgggtcgc gcaggcgcgg cagcaattgg gtcacggcgg
      661 ccggcatttc gccgcgcgtg cgcagcacct cgcggtggta ggcctcggcg gcgtcgagca
      721 gcaggatggt gtggccggtc ggcgcggtgt cgaggacgac gaaaccgtcg ttgccgcgat
      781 cgacctcacg ggcaaaggcg cggaagacgg cgatctcctc ggtgcatggc gagcgcaaat
      841 cctcttccag cagcgccagc gcactgtcgt cgagcgcgcc gcgcgccttg ctcagcacct
      901 cttcggtgta ggccgcgact tcggcggtcg ggtcgatgcg gctgagcgag aggccggcaa
      961 tttcaccatc gaccgccgcc gcgacgtgcg ccgccgggtc ggtggtggtc agatgcaccc
     1021 ggtggccgcg gcgggccagc gccacggcaa cggcggcagc gaccgtcgtc ttgccgacgc
     1081 cgcccttgcc catcgtcatc acgacaccgc gtccggcgct ggccagttcg tcgatcaagg
     1141 cagcgagacc gcccggcagc ttcgtgctcg tggccggcgc tacctcgggc gcggcggccg
     1201 gggcgggggc gacgatggcg cgcagggcgg ccaggccgac cgtgccgcgc ggcaggaaag
     1261 ggacgacccg ccggggcagg gcacccaggc cgcctggcat ggcgagcagc gcggcagtgc
     1321 tgcgctgttc catggcgagg gcgacggggt cgttgccggc ggcataaaac aggccgttga
     1381 tcgccagttg cagattggca acgcccagtt cggccagttc gtggcgcgtc cgttcggctt
     1441 cgcgcagggc ggcggcgtcg gcccgggcga ccaggacgac acgcgtccgt gccggatcgg
     1501 acagccgctc gacggtggcc gcatagagcg ctttctgttt ctccaggccg gccagcggcc
     1561 cgagacagga ggcgccgccg tggctgctgt cgatgtaacc actccaggcc gaaggcagcg
     1621 tcagcaggcg cagtgtgtga ccggtcggcg ccgtatcgaa gatgacgtgg tcgaagtcgg
     1681 cggtcgacgc cggctcgccg agcagtttgg cgaactcgtc gaaagcggcg atctcgaccg
     1741 tgcaggcgcc ggagaattgt tcttccatgc tctggatcgc cgccgccggc aggatgccgc
     1801 ggtagggcgc gaccatccgc tcgcggtagg cggccgccgc cgcttccggg tcgatgttga
     1861 gggcgctcag gccgggcacg ccgcgtaccg gcgtcggcgt gccggcgagt ggcgtttcca
     1921 ggacttcatc gaggttggag gccgggtcgg tgctgacgat cagcacgcgt ttgccggttt
     1981 cggccagggc caggccgctg gcgcaggaga gcgaggtctt gccgacgccg cccttgccgg
     2041 tgaagaagag gtagcgtggc gcctggtcga gatcgagcat gggttttcct cagcagcagc
     2101 cggatttcgg cgaacagcag gactttttcg gcgcctcttc gctgctgccc agggtcaggc
     2161 cgagtttctg cgccagttgg gcgcgcgtcg gataaaggcc gctgctgacg atcttgccgt
     2221 cgatgacgac gatcggcagg cggtcgatgc cggcttccat ttccttgacc accgccggat
     2281 tggcagcgaa ggcctgcggc tcctggccga ggttgtagcg ctggacggca acgccctgct
     2341 cgccgaccca ggcgaggtcg gcggcgaact ggaccagcac gggatcgaca tcgacgccgc
     2401 agacgccggt cggacagcac atggcgggat cgtaaacttc aagtttctgc atcttcttct
     2461 cctcgtttca aagggatttt tcgtaccagg tcttggtcga attgaccatc ttcaccagcc
     2521 acagcatcac aggtacctcg atcagcacgc caaccacggt ggccagcgcg gcgccggaat
     2581 cgaaaccgta gagcacgatg gcgacggcga ccgccagctc gaagaagttc gaggcaccga
     2641 tcatcgtcgc cgggccggca acgtcgtgct ggacgcgaaa cttgtggcac agccagtagc
     2701 cgatggcggc gatcaacaag ccctggagca ggatcggcac ggccagcatg gcgatgatca
     2761 gcggctggcg gacgatggcc tcgccctgga aagcgaagag caggaccagc gtcgccagca
     2821 gcgccacgat ggtgaacgga ccgatccgtt cgatggcccg cttgaaggcc gcttcgccgc
     2881 cgcgcaggaa atgcttgcgc agcccctggg cgatggccag gggaatgacg atgtacatca
     2941 ccaccgacag cagcagcgtg tcccacggta ccgggatggc ggagacgcca aggagcagcg
     3001 cgacgatggg ggcaaacgcg aagaccatca ccagatcatt cagtgccacc tgcgagatcg
     3061 tgaagttggc gttgccccgg cacaggttgc tccacacgaa gaccatcgcc gtgcacggcg
     3121 ccgcgccgag caggatcagg ccggcgatgt agctgtcaag ttgttcagcc ggcaggtaag
     3181 gggcaaagac gtaacggatg aacagccagc cgagcgccgc catcgtgaac ggcttgatcg
     3241 cccagttgac gaacaacgtg atgccgatgc cggccttctg gtcctttact tcatggatcg
     3301 tcgcgaaatc gatcttcatc agcatcggta cgatcatcac ccagatcagc aagccgaccg
     3361 gcaggttgac ctgggcgact tccattccgc ccagggcctg gaaggcgccc gggaaccact
     3421 gaccgagcag gacgccagcg acgatgcaga gcaccaccca gagactgagc caacgctcga
     3481 aaaagcccat cggggcgccg gctgcctgtt tcgcggcaac gccgcattgc gcgctcattg
     3541 ttcgcactcc tggtgaatcc gctgtgccgc catcttgatt tcggcggcgc tcatggtttc
     3601 cagcggcagt tggagaaaag cctcgacgcg ctggcgcagc acttggtaag cgcgctcgaa
     3661 ggcagcctgg cgggccgcca gcggttcgac gtgggccggg tcggcgatgc cccagtgggc
     3721 ggtggtcgga tggccgggcc agatcgggca ggtttcgccg gccgccgagg cgcagacggt
     3781 gaaaatgtag tcgatggccg gcgcgccggg ggcggcgaat tcatcccatg atttgctgcg
     3841 cgcttcgggc tgcggcacgc catggcttgc cagggtttcg agcgcgaccg gattgaccgt
     3901 gccggtcggc tggctgccgg ccgaaaaggc gcggatgcga ccgccgccaa ggtggttgaa
     3961 caaggcctcg ccgaggatgg agcgggcgga gttgccggtg cataacacga gaacggtttt
     4021 catccgtcgt tcctcagctc ttttcgcggc cgataccgtc gagcttgtcc tgcagcgcca
     4081 tcgcgtcgag cttgtcgatc ggcagggcaa ggaatagttc gatgcgtcgt ttcaactgga
     4141 tgctcgcctt catgaccgct tcgcgccgtg cttcgtcatc gccgccgacg gcggccgggt
     4201 cctcgacacc ccagtgggcg gtcatcggtt ggccgggcca gaccgggcag acttcgccgg
     4261 ccgcgttgtc gcagacagtg aagatgaagt cgaggcgagg tgcgtcggca ctggcgaatt
     4321 cgtcccagct cttgctgcgc agttgcgcgg tcggcaaatg ctgacgttcg agcagttcgc
     4381 gggcgtaggg attgaccgtg ccggtcggcc ggctgccggc cgagtaggcg cggaaacgct
     4441 ctttgccgag gtggttgagc aaggcttcgg ccaggatgct gcgcgccgaa ttgccggtgc
     4501 acaggaaaag cacgttgtag atgcggtcgt tcatttgggg atctccgtcg ggtaaggggg
     4561 gcgggcggcg tttctcgatg ctgtcgcagg cgctcgtcag cggcaggcag ttgctgccct
     4621 ggcagcagtt gtgggtgagg aaggcgagca gttcgtccat tgccgggtaa caggcggaat
     4681 agatgatgct gcgcccgtcg cgccggcttt cgatgaggcc gacccggctc aggtgggcga
     4741 aatgaaagga agcggtggcc cccgccaggt ccagcgtgcg ccccagcgca ccggcgctca
     4801 ggccggcggg gccggcttcg acgagcaggc ggaagatggc gagccgggat tcgttgccga
     4861 gggcggcgag cttttcggtg gcggttttga tttccatatt tccaataata tcgaaatgcg
     4921 aagcgaaatt ttatttcgct gaggtgacat ttttttgacg aaaaaattgc gccagggtct
     4981 ttgtcatccg gctgtaacaa aagcttccta gactgcgttc gtccatttca cccacagaag
     5041 gaaccagaca tgttgcaatc ccgtatcttc cgcgcttccc tgctcgccct gtcgctgggc
     5101 ctcgccggct tcggcgccca ggccgccgaa atcaccggtg ccggcacttc cttcccggcc
     5161 ccggtttacg ccaaatgggc cgatgcctac cagaaggcga ccggcaacaa ggtcaattac
     5221 cagtcgatcg gctccggcgg cggcatcaag cagatcaacg ccaagaccgt cgatttcggc
     5281 gcctccgaca tgccgctcaa gcaggaagtg ctcgacaagg acggcctggt ccagttcccg
     5341 accgtgatcg gcggcgtcgt gccggtggtc aacgtggtcg gcgtcaaggc cggcgacctc
     5401 cgcctgaccg gcgaaatcgt cgccgacatc tacctcggca agatcaccaa gtggaacgac
     5461 aaggcgatcg ccgaactgaa cccgaacgtc aagctgcctg gcgacgacat cgccgtcgtt
     5521 cgtcgcgccg acggttcggg caccaccttc atcttcacca actacctgtc caaggccagc
     5581 agcgactgga agagcaaggt cggcgaaggt acggccgtgc agtggccgac cggcatgggc
     5641 ggcaagggca acgaaggcgt tgccgccttc ctgcaacgca tcccgaactc gatcggttac
     5701 gttgaatacg cctacgccaa gcagaaccac ctggcctgga cctggctgaa gaacccggcc
     5761 ggcgaattcg tccgtcccga cgacgacaac ttcaaggccg ccgccgctgg cgccgactgg
     5821 agcaagtcgg acttcggcga aatcctgacc gcccaatcgg gcaagggtgc ctggccgatc
     5881 agcggcgcca ccttcatcct gatgcacaag gttcaggaca agccggcgca ggccgctgaa
     5941 gtgctgaagt tcttcgaatg gtcgtacaag aacggcgcca agctggcttc cgacctcgac
     6001 tacgtgccgc tgccggatag cctggtgaag cagattgccg cttcctgggc caacatcaag
     6061 gacgcttccg gcaaggccgt caagtaagcg gggccagcgt tgaaccggac gcctccggtc
     6121 ctagtggctg gaggcgtttt ctttcgcgcc gggcgcgaaa aaccgcaaga cccttaatcg
     6181 atacggacat tcgtcatgag tgaaacacaa gccaaagggc gactggcaga cctgttgttc
     6241 ttcaacctga cgcgcacggt ggcgttcatc accctggccc tattgggcgg gatagtgatt
     6301 tcgctgacct ggagcgccct gccgtcgatc gagaaattcg gcttcagctt cctgaccagt
     6361 tcggcctgga atccgccggc cgacgatttc ggtgctctgg tgccgatcta cggcaccatc
     6421 atcacctcga tcatcgcgct ggtcattgcc ctgccgatca gcttcggcat cgccctcttc
     6481 ctcaccgaaa tggcgccgac ctggctgcgc cgcccgctcg gcatcgccat cgaactgctc
     6541 gccggcatcc ccagcatcgt gtacggcatc tggggcctgc tcgttttcgc gccgatcttc
     6601 ggccagtacg tgcaaccctt catcgcccag acgctgggca agctgccgct gatcggcgcg
     6661 ctgttctccg ggccgtcgct cggcatcggc atcctcgccg ccggcatcat cctggcgatc
     6721 atgatcattc ccttcatcgc ctcggtgatg cgcgacgtct tcgagctggt gccgccgatg
     6781 ctcaaggaat cctgctacgg ccttggtggc acgacctggg aaacggtgag caaggtggtc
     6841 ctgccctaca cccgggtcgg cgtcatcggt ggcgtcatgc tcggcctcgg ccgcgccctt
     6901 ggcgaaacga tggcggtcac cttcgtcatc ggtaacgcca gtctgctcgg cagcttctcg
     6961 ctgttcatgc cgggcaacag catcacctcg gcgctcgcca acgaattcgc cgaggcgacg
     7021 cccggtctgt atacggcggc gctggtcgaa ctcggcctga tcctgttcgt catcaccttc
     7081 atcgtgctct cgctgtccaa actgctgctc ctgtcgatgg aaaagcagaa cggcggcggc
     7141 gcccactaag gaatcggtca tggacctgag aacccgtcgt aaaaccacca acgtcatcgc
     7201 cctcggcctg tcgctggcgg ccatggcctt cggcctgttc tggctgatct ggatcctctg
     7261 gatggtcttt gaacagggca tcggcgccct gtcgctcgat ctgctgacgc tcgacacgcc
     7321 ggcaccgggc aacgccggcg gcctgcgcaa cgcgatcttc ggcagcctgg tgatggtcgg
     7381 cctggcgatg ctgatcgcca cgccggtcgc catcctcgcc ggcatctacc tcgccgagta
     7441 cggtcggcgc acggtgctcg gccaggtgac gcgcttcgtc aacgacatcc tgctgtcggc
     7501 gccttcgatc gtcatcggcc tcttcatcta tgcgatctac gtcgcccagg tcggcaagtt
     7561 ctccggcatg tccggcgcgc tggccctggc cctgctggtg attccggtcg tcgtccgcac
     7621 caccgaggac atgctgatcc tggtgccgaa cgccctgcgc gaagccgcct tcgcacttgg
     7681 cgcgccgaag tggaaggtga tcctgatggt caccctgcgc tcggccaaga ctggtgtcat
     7741 caccggcatc ctgctggcgc tggc
//
LOCUS       DAOSDX010000015         7576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3563_length_7576_cov_3.234543, whole genome shotgun sequence.
ACCESSION   DAOSDX010000015 DAOSDX010000000
VERSION     DAOSDX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_7576_cov_3.234543"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2821)
                     /locus_tag="PLN02_00685"
     CDS             complement(<1..2821)
                     /locus_tag="PLN02_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOI50866.1"
                     /translation="MSAEIAARGQGLRGAYRSTGILLLVCAGLLPARAALAQSWHAAD
                     GSLSADREQAAASWRADAEFNGNWGLAAIRAEYAYAQGYAGLGARIGVLDQPVWNAHP
                     EFAASVAGDPKLSFITTRGIRTYDDPYLPFRAGDPFVYDGQLYINGYGGIATHGTHVA
                     GIAAANRDTVAGRPGVMQGVAFAARVFAADVGDPGPEDGIVRGNDGGTYLAAWRAMID
                     SRVDVITNSWGIGITWGGGWQFVDAGKQFLEIQSLLGTPAGGAYDGALQAARSGIVVE
                     FSAGNESGQQPDAMAGLASFVPAIENNWITTMSLARTASGLQASGFSTICGYTRYYCV
                     AAPGSAINSSVATGDISGLHAGDPVRDPLAAYEALSGTSMAGPFVSGAIGVLKGRYPY
                     LANGDLAQILKTTARDLGAPGVDEVYGWGLIDLQKAMDGPGQLMSRLVAELPAGTLDV
                     WANDIGDAALRQRQAEAQVEIAAWRQRKIDMGWGNGWASVDTASLSERLYVEYRPLAS
                     QARVLARQLMVARATPPYAGFWTALAAVENDPLASTIVKEYGKIEAWLPLGPDLARFE
                     RFLASRYPDDETLAREVAAAKVQGLAQEYLVQEARIASLLAATYENGLTKRGAGELRL
                     TGNNSYGGETRVEAGRLRVDGALSGAVAVYGQGILGGNGRIGSLLAQAGGTVAPGASI
                     GTLSVAGDARFESGSLLAVEFSGDGRSDHLAVAGKATLAGGLLGVQAEDASVALETFV
                     AAAAGRHAYTFLSAGGGIEGQFAGALSPYAFFRPRLSYAADRVTLDIERLPFASAGRN
                     FNERQTAAGIDSLAPANPLYAQVLAAPAGFVAGPLFAALSGDIHPTAGGVLLAAGQVQ
                     RDAALDRLRLVETQSASAPRTWGQVYGRRGRADGDGNAAGYDNDGDGFFVGGDSLLDA
                     GWTLGFLVGHENGGVRTVNARADTGS"
     gene            3072..4103
                     /gene="dusB"
                     /locus_tag="PLN02_00690"
     CDS             3072..4103
                     /gene="dusB"
                     /locus_tag="PLN02_00690"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOI50867.1"
                     /translation="MDFVGFTLRNNLFVAPMAGVTDRPFRQLCKKLGAGLAVSEMVTS
                     NSLLYGSAKTLRRANHVGEVAPISVQIAGADPQMMAEAAKHNVDNGAQIIDINMGCPA
                     KKVCNVMAGSALMQDEQLVGRILDAVVAAVPNTPVTLKFRTGWNLVNKNAPTIARIAE
                     SAGVRAVAIHGRTRCQQYTGEAEYDTIAMVKTLIRIPVIANGDITTPEKARYVLDHTG
                     ADGVMIGRAAQGRPWLFREIEHYLKTGEHLPPASVAEIHGILLAHLEDLYDFYGLETG
                     VKVARKHISWYTKGLVGSAAFRREMNTLPTIEQQRQAVDEFFRHQAAAGEHLHYETTD
                     HQDREALAA"
     gene            4100..4333
                     /locus_tag="PLN02_00695"
     CDS             4100..4333
                     /locus_tag="PLN02_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HOI50868.1"
                     /translation="MSQHDLGRCVINALEQYFRDLDGEKPAAIYDMVLKSVEKPMLEL
                     VLAKAGTNQTLAAEMLGINRNTLRKKLTEHQLL"
     gene            4340..5932
                     /gene="purH"
                     /locus_tag="PLN02_00700"
     CDS             4340..5932
                     /gene="purH"
                     /locus_tag="PLN02_00700"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOI50869.1"
                     /translation="MTIKQALISVSDKTGVLEFAQGLAAQGVKLLSTGGTAKMLRDAG
                     LAVTEIGDYTGFPEMLDGRVKTLHPKVHGGILARRDLPEHLATIEEHGIPTIDLVCVN
                     LYPFAATIAKAGVTLADAIENIDIGGPAMVRSSAKNYAGVAIVTDPADYAPLLDEMKA
                     NGGALQLGTRFNLAKKAFTHTARYDSMIANWLTGLDAGAEAQPEAAAPIPAVFPAKLQ
                     LAFDRVETLRYGENSHQSAAFYRDTSALPGSIANYTQLQGKELSYNNIADSDAAWECV
                     KAFDTPACVIIKHANPCGVAIDANLLGAYEKAFQTDSTSAFGGIIAFNGEVGIEVVEA
                     MNERKHFVEVLIAPSFTAEAKAALAAKANLRVLVVPLGRDLNKLELKRVGGGLLVQTA
                     DDFTAQVKDLKVVTKVQPTLRQIEDLLFAERVAKFVKSNAIVFCGGGMTLGVGAGQMS
                     RVDSTKIACIKAQNAGLELKGSVVASDAFFPFRDGLDVLAEAGAKAVIQPGGSMRDEE
                     VIAAADEHGIAMVFTGARHFRH"
     gene            6062..7339
                     /gene="purD"
                     /locus_tag="PLN02_00705"
     CDS             6062..7339
                     /gene="purD"
                     /locus_tag="PLN02_00705"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOI50870.1"
                     /translation="MKLLVIGSGGREHAMAWKLAQTPGLQKVYVAPGNAGTAREHELE
                     NVNITDPVALADFAEQNKVHLTLVGPEAPLAAGVVDIFRARGLKIFGPTKAAAQLESS
                     KDFAKRFMARHNIPTAGFETFSDAAAAHAYIDKQGAPIVIKADGLAAGKGVVVAMSLD
                     EAHAAIDDMLSGNKLGDAGARVVIEDFLDGEEASFIVMVDGKNVLALASSQDHKRIFD
                     GDQGPNTGGMGAYSPAPCVTPEVHAKAMREIILPTVRGMLADGIPFTGFLYAGLMIGK
                     DGSLKTLEFNCRMGDPETQPILMRLKSDFVKLLEHGINGTLDQIEAEWDRRVALGVVL
                     AAANYPETPKKGDVIVGLPKGNSFGDDCHVFHAGTADQDGQVVTNGGRVLCVTALGEN
                     VRLAQKAAYEAAAQISWDGLQYRKDIGHRAISR"
BASE COUNT         1355 a         2684 c         2351 g         1186 t
ORIGIN      
        1 agctgccggt atcggcacgg gcgttcaccg tcctgacgcc gccgttctcg tggccgacca
       61 ggaagccgag cgtccagcca gcgtcgagca ggctgtcgcc gccgacgaaa aagccgtcgc
      121 catcgttgtc gtagccggcg gcattgccat cgccgtcagc ccggccgcgc cgcccgtaca
      181 cttggcccca ggtgcgcggc gcgctcgccg actgcgtttc gaccagacgc agacgatcca
      241 gcgccgcatc gcgctgaacc tgaccggcgg cgagcaatac gccgccggcg gtcgggtgga
      301 tgtcgccgga gagcgccgcg aacaaggggc cggcgacaaa accggcgggt gcggccagca
      361 cctgcgcgta cagcggattg gccggcgcca gactgtcgat gccggcggcg gtctgccgct
      421 cgttgaagtt gcgcccggcg ctcgcgaagg gcagacgttc gatgtccagg gtgacgcggt
      481 cggcggcata gctcaggcgg ggccggaaga aggcatacgg cgacagcgcg ccggcgaact
      541 gcccttcgat gccgcccccg gcgctcagga aggtgtaggc atgtcgcccg gccgcggcgg
      601 cgacgaaggt ctccagcgcg acgctggcat cctcggcctg cacgccgagc agtccgccgg
      661 ccagcgtcgc cttgccggcc accgccaggt gatcggaacg cccatcaccg ctgaactcga
      721 cggcgagcag cgagcctgac tcgaagcgcg cgtcgccggc aacgctcagg gtaccgatcg
      781 acgcccccgg cgccaccgtg ccgccggcct gcgccagcag cgagccgatc cggccgttgc
      841 cgcccaggat gccctggccg tagaccgcaa cggcaccgct cagcgcgcca tcgacgcgca
      901 gacgcccggc ctcgacccgg gtttcgccgc cgtagctgtt gttgccggtc agccgcagct
      961 cgccggcacc gcgcttggtc aggccgtttt cataggtcgc ggcgagcagg ctggcgatcc
     1021 gcgcttcctg gacgaggtac tcctgcgcca gcccctggac cttggccgcc gccacttccc
     1081 gggccagcgt ctcgtcgtcc ggataacggc tggcgaggaa acgctcgaag cgcgccagat
     1141 cgggccccaa gggcaaccag gcctcgatct tgccgtactc cttgacgatc gtcgaagcca
     1201 gcggatcgtt ctcgaccgct gccagcgccg tccagaagcc ggcgtagggc ggcgtcgcac
     1261 gcgccaccat caactgccgg gcaagcaccc tggcctggct cgccagcggt cgatattcca
     1321 cgtaaaggcg ttcgctcagg ctcgcggtat cgacgctggc ccagccgttt ccccacccca
     1381 tgtcgatctt gcgttgtcgc caggccgcaa tctccacctg cgcttccgcc tgacgctggc
     1441 gcagcgcggc atcaccgatg tcgttggccc agacatcgag cgtgccggcg ggcagctcgg
     1501 cgaccaggcg gctcatcaac tggccgggac cgtccatcgc cttctgcagg tcgatcagcc
     1561 cccagccgta gacttcgtcg acgccgggcg ccccgaggtc gcgcgccgtc gtcttgagga
     1621 tctgcgcgag atcgccgttg gcaagatagg gatagcgccc cttcaagacc ccgatggcgc
     1681 cggaaacgaa ggggccggcc atcgacgtgc cgcttaacgc ctcgtaggcg gcaagcggat
     1741 cgcgtaccgg atcgccggca tgcagccccg agatgtcgcc ggtcgccacc gaactgttga
     1801 tggcgctgcc gggcgcggcg acgcagtaat acctggtgta accgcagatc gtcgaaaaac
     1861 cgctggcttg caggccgctg gcggtacgcg ccagactcat cgtcgtgatc cagttgtttt
     1921 cgatcgcggg aacgaagctc gccagaccgg ccatcgcatc gggctgctgg ccgctttcgt
     1981 tgccggccga aaactcgacg acgataccgc tgcgcgccgc ctgcagcgcg ccgtcgtagg
     2041 cgccgccggc cggcgtcccg agcagggact ggatttcgag gaactgcttg ccggcatcga
     2101 cgaactgcca accgccgccc caggtgatgc cgattcccca actgttggtg atgacatcga
     2161 cccggctgtc gatcatcgcc cgccaggccg ccaggtaggt gccgccgtcg ttgccgcgga
     2221 cgataccgtc ctccggtccg ggatcgccga cgtcggcggc gaagacgcgc gcggcgaagg
     2281 ccaccccctg catgacgccg gggcgaccgg cgacggtgtc gcggttcgcc gccgcaatgc
     2341 cggcgacgtg tgtgccatgc gtggcaatcc cgccatagcc gttgatatag agttgcccgt
     2401 cgtagacgaa ggggtcgccg gcgcggaatg gcagataggg gtcgtcgtag gtacggatgc
     2461 cgcgcgtggt gatgaagctc agcttgggat cgccggcaac gctggcggca aattcgggat
     2521 gggcgttcca taccggctgg tcgagcacgc cgatgcgcgc gcccaggccg gcgtaaccct
     2581 gagcataggc gtattcggca cgaatcgcgg cgaggcccca gttgccgttg aattcggcat
     2641 cggcccgcca acttgccgct gcctgctcgc gatccgcgct cagactgccg tcggcggcgt
     2701 gccaggattg ggcgagcgcc gcccgcgccg gcagcaagcc ggcgcagacc agcagcagaa
     2761 tgccggtcga tcggtaagca ccgcgcagac cttgcccccg agccgctatt tccgcagaca
     2821 tcatttcccc ttttgcttcc gccatccggc ccttgccgac attccccgat gcctggtgcg
     2881 ccgctcccgg cgacgacatt accatggcat tgccgggcgc cgccaccgga tagccctgtg
     2941 ccaggatagg ccgcgacgaa gatcccggca tgccggcaag cgccggcaag tccgaaactg
     3001 actatttttt aggcagcgat tcagctataa tccgcccctt ccccgtttgt cccgcccccg
     3061 cccgttcgcc catggatttc gtcggtttca ccctgcgcaa caatctcttc gttgcaccca
     3121 tggcgggtgt caccgatcgc cccttccgcc agttgtgcaa gaagctgggc gccggactgg
     3181 cggtgtccga gatggtgacc tcgaactcgc tgctctacgg cagcgccaag acgctgcgcc
     3241 gcgccaacca cgtcggcgaa gtggcaccga tctcggtgca gatcgccggt gccgacccgc
     3301 agatgatggc cgaagcggcg aaacacaacg tcgataatgg cgcccagatc atcgacatca
     3361 acatgggctg cccggccaag aaggtgtgca acgtgatggc cgggtcggcg ctgatgcagg
     3421 acgagcaact ggtcggccgc attctcgacg ccgtcgtcgc cgccgtgccg aataccccgg
     3481 tgacgctgaa attccgcacc ggctggaacc tcgtcaacaa gaacgcgccg accatcgccc
     3541 gcatcgccga gagcgccggc gtgcgcgccg tcgccatcca cggccggacg cgctgccagc
     3601 aatacaccgg cgaagccgag tacgacacca tcgccatggt caagacgctg atccggattc
     3661 cggtaattgc caacggcgac atcaccaccc cggaaaaagc cagatacgtc ctcgaccaca
     3721 ccggcgccga cggcgtcatg atcggccgcg ccgcgcaggg ccggccgtgg ctgttccgcg
     3781 agatcgagca ctacctgaag accggcgagc atctgccgcc ggccagcgtc gccgagatcc
     3841 acggcatcct cctcgcccac ctcgaagacc tttacgactt ctacgggctg gaaacgggcg
     3901 tcaaggtcgc ccgcaagcac atctcctggt acaccaaggg actggtcggt tcggccgcct
     3961 tccgccggga gatgaacacc ttgccgacca tcgaacaaca gaggcaggcg gttgacgaat
     4021 tcttccgcca ccaggctgca gcaggggaac acctgcatta cgaaacgacg gaccatcagg
     4081 atagggaggc gttggcagca tgagccaaca tgacttgggg cggtgcgtga tcaacgcact
     4141 ggagcagtat ttccgggacc tcgacggcga gaagccggcg gcgatctacg acatggtcct
     4201 gaagagcgtc gaaaagccga tgcttgaact ggtgctggcc aaggccggca ccaaccagac
     4261 gctggcggcg gagatgctgg gcatcaaccg caacacgctg cgcaagaaac tcaccgaaca
     4321 tcaacttctg taaatcgcca tgaccatcaa gcaagcgctg atttccgtct ccgacaagac
     4381 gggtgtactc gaattcgccc aaggcctcgc cgcgcaaggc gtcaagctgc tgtccaccgg
     4441 cggcaccgcc aagatgctgc gcgacgccgg cctcgccgtc accgaaatcg gcgactacac
     4501 cggcttcccg gaaatgctcg acggccgcgt caagaccctg catccgaagg tgcatggcgg
     4561 catcctggcc cgtcgcgacc tgccggagca cctggcgacc atcgaagaac acggcattcc
     4621 gaccatcgac ctggtctgcg tgaacctcta cccgttcgcc gccaccatcg ccaaggccgg
     4681 cgtgacgctg gccgatgcca tcgagaacat cgacatcggc ggcccggcca tggtccgttc
     4741 ctcggccaag aactacgctg gcgtcgcgat cgtcaccgat ccggccgact acgccccgct
     4801 gctcgatgaa atgaaggcca acggcggcgc cctgcaactc ggcacccgtt tcaacctggc
     4861 caagaaagcc ttcacccaca ccgcccgcta cgacagcatg atcgccaact ggctgaccgg
     4921 cctcgacgca ggcgccgaag cccaaccgga agccgccgcg ccgatcccgg ccgtcttccc
     4981 agccaagctg caactggcct tcgaccgcgt cgaaaccctg cgttacggtg aaaactcgca
     5041 ccagtccgcc gccttctacc gcgacaccag cgcgctgccg ggcagcatcg ccaattacac
     5101 ccagttgcag ggcaaggaac tgtcctacaa caacatcgcc gattccgacg ccgcctggga
     5161 atgcgtcaag gccttcgaca cccccgcctg cgtcatcatc aagcacgcca acccctgcgg
     5221 cgtggccatc gacgccaacc tgctcggcgc ctacgaaaag gccttccaga ccgattcgac
     5281 ctcggctttc ggcggcatca tcgccttcaa cggcgaagtg ggtatcgaag tcgtcgaagc
     5341 catgaacgag cgcaagcact tcgtcgaagt gctgattgcc ccgtccttca ccgccgaagc
     5401 caaggccgcc ctggccgcca aggccaacct gcgggtgctg gtcgtgcccc tgggccgtga
     5461 cctgaacaag ctggaactga agcgcgtcgg cggcggcctg ctggtgcaga ccgccgatga
     5521 cttcaccgcc caggtcaagg acctgaaggt ggtgaccaag gtgcagccca ccctgcgcca
     5581 gatcgaagac ctgctcttcg ccgagcgcgt cgccaagttc gtcaagtcca acgccatcgt
     5641 cttctgcggc ggcggcatga cgctcggcgt cggcgccggc cagatgagcc gcgtggactc
     5701 caccaagatc gcctgcatca aggcgcagaa cgccggcctg gaactcaagg gttcggtcgt
     5761 cgcgtcggat gccttcttcc cgttccgcga cggcctggat gtgctggccg aggccggcgc
     5821 caaggccgtc atccagccgg gtggctcgat gcgcgacgag gaagtcatcg cggcagccga
     5881 cgaacacggc atcgccatgg tcttcaccgg tgcccgccac ttccgccatt aatttcgttc
     5941 tcggtacgcc cgtgactttg tcgacttcgc cttgccgtgc tgatagcact gtctgcggct
     6001 cgtcttcgcg tcacgagcgc cccgtgaacg aaatcacgct ctggtataac aaggaaaaac
     6061 catgaaacta ctggttatcg gctccggcgg tcgcgagcac gccatggcct ggaagctggc
     6121 gcagacgcca ggcctgcaga aggtttacgt cgcccctggc aacgccggca cggcgcgcga
     6181 gcatgaactg gagaacgtga atatcaccga tccggtagcg ctcgccgatt ttgccgagca
     6241 gaacaaggtg cacctgacgc tggtcggccc cgaggcgccg ctggcggccg gcgtggtcga
     6301 tatcttccgt gcccgtggcc tgaagatctt cggcccgacc aaggcggccg cccagctcga
     6361 atcctccaag gatttcgcca agcgtttcat ggcccgccac aacatcccga ccgccggctt
     6421 cgagaccttc tccgatgccg ccgccgccca cgcctacatc gacaagcagg gcgcgcccat
     6481 cgtcatcaag gccgacggcc tggccgccgg caagggcgtt gtcgtcgcga tgagcctcga
     6541 cgaagcacac gccgcgatcg acgacatgct ctccggcaac aagctcggcg atgccggcgc
     6601 ccgcgtcgtc atcgaggatt tcctcgacgg cgaggaagcc agcttcatcg tcatggtcga
     6661 cggcaagaat gtcctcgcgc tggcctccag ccaggaccac aagcgcatct tcgacggcga
     6721 ccagggcccc aacaccggcg gcatgggcgc ctactcgccc gccccctgcg tgacgccgga
     6781 agtgcacgcc aaggcgatgc gcgaaatcat cctgccgacg gtgcgcggca tgctcgccga
     6841 cggcatcccc ttcaccggct tcctctacgc cggcctgatg atcggcaagg acggctcgct
     6901 caagacgctg gaattcaact gccgcatggg cgacccggaa acccaaccga tcctgatgcg
     6961 cctcaagtcc gacttcgtca aactgctcga gcacggcatc aacggcacgc tcgaccagat
     7021 cgaagccgaa tgggaccgcc gcgtcgcact cggcgtcgtg ctcgccgccg ccaactatcc
     7081 ggaaacgccg aagaagggcg acgtgatcgt cggcctgccc aagggcaaca gcttcggtga
     7141 cgactgccac gtcttccacg ccggcaccgc cgaccaggac ggccaggtcg tcaccaacgg
     7201 cggccgcgtg ctgtgcgtca ccgcgctcgg cgaaaacgtc aggctcgccc agaaagccgc
     7261 ctacgaagcc gccgcccaga tcagctggga cggcctgcaa taccgcaagg acatcggcca
     7321 ccgcgcgatc agccgctgaa cgcgtcagcc gtctgcgaac cggcatcggg gtggctgccc
     7381 ggatgccggt tttttattcg ctttgcggct attggatttt atgaagcgct gttttccgct
     7441 gttggccggc gggctgcctt ggtttctgac gcgccgtttt ccttcccgga cggacgggcg
     7501 tttggttttc tgaagcgctg ggttccgcgc ctgacgcgcg ggcgtacttt ttcttgtttg
     7561 gccaagaaaa agtagc
//
LOCUS       DAOSDX010000016         7515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3597_length_7515_cov_2.994370, whole genome shotgun sequence.
ACCESSION   DAOSDX010000016 DAOSDX010000000
VERSION     DAOSDX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3597_length_7515_cov_2.994370"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLN02_00710"
     CDS             <1..470
                     /locus_tag="PLN02_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOI50871.1"
                     /translation="TFGVGGELDEREWKSVFRQLAAAGLVHVDMTEHGALQLTEAARE
                     VLKGRRSVQLRRPTRKRSAPSKTSRAPVVDLAPADAALFQRLRQWRADTAREQGVPAY
                     VILHDKTLRELAECRPVSHGLLAGITGMGSAKIEHYGEELLQLIREEARHEAS"
     gene            457..1074
                     /locus_tag="PLN02_00715"
     CDS             457..1074
                     /locus_tag="PLN02_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HOI50872.1"
                     /translation="MKHPDRLTAILGSIVIAALIVSGIAPYDRATWLMEVAPVLLALP
                     LLWLTRRSFPLTALLYVLIAIHALVLIGGGAYTYARVPFGFWLQDLLGTTRNPYDKVG
                     HFLQGFVPALVAREILLRDDWVRGAKMTAFLCICIALAISACYELIEWAAALAMGQGA
                     DEFLGTQGDPWDTQSDMFLALLGAIAALLLLSGWHNRQLHRLRRG"
     gene            complement(1084..2742)
                     /locus_tag="PLN02_00720"
     CDS             complement(1084..2742)
                     /locus_tag="PLN02_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HOI50873.1"
                     /translation="MPVKIVGATPSAYQYPLLIKQLLLTPLATAPDQEITYQGRLRYT
                     YRELRQRIGRLANVLSSLGVEAGHTVAMMDWDSHRYLESFFAVPMMGAVLQTVNVRLS
                     PEQILYTLNHARADVVMVNSEFFPILAEIAGRLESVRAFILISDEAVLPASPVVAAGE
                     YEAMLAAAEANYDFPDFDENAQATTFYTTGTTGNPKGVYFSHRQLVLHTLGIASGTGN
                     AAGQGRLHREDVYMPLTPMFHVHAWGFPYLATMLGLKQVYPGRYQPDVICRLVEEEGV
                     SFSHCVPTILHMVFGHPAAAKTDFSGWKLVIGGAAMPEAMAGAALARGIDLFTGYGMS
                     ETCPVLTLAHLASADLEKSPAEQAVIRAKTGRPLPLVDLRIVDAQMRDVAHDGKATGE
                     VVVRSPWLTQGYLHAPEASEELWAGGYLHTADIGNIDAAGYLKVTDRIKDVIKTGGEW
                     TSSLQLEDIVAKHEAVQEVAVIGVADDKWGERPLALVLLKPEFVGQVSEHALRNYAAH
                     AIEAAGVSRYGVLLQVRFVKTLVKTSVGKMNKRLMRADPDALCL"
     gene            complement(2816..4090)
                     /locus_tag="PLN02_00725"
     CDS             complement(2816..4090)
                     /locus_tag="PLN02_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI50874.1"
                     /translation="MQFSELGLSADIQRAVAEQGYDTPTPIQQQAIPAVLAGHDLMAT
                     AQTGTGKTAGFTLPILQRLSAGANDRLSRVARTPRVLVLTPTRELAIQVEESVRTYGK
                     HLPINSLAVFGGVGINPQIAGLRRGVDILVATPGRLLDHVQQKTVDLGHIEIFVLDEA
                     DRMLDMGFIRDIRKIIALLPKQRQNLLFSATFSPEIRELAGGLLHQPVSVDVAPRNTA
                     AETVAQRVIECNREQKKDLLKHLFETRSLHQVLVFARTKHGADALARALDKAGIKAAA
                     IHGDKSQGARTRVLGEFKEGKLAALVATDIAARGIDIDALPYVINYELPNVPEDYVHR
                     IGRTGRAGMEGEAISLVCHDERPQLKDIEKLIKRNLERVVIEGFEPAAHAAPRPPQPP
                     RGGRSAQPARAPAKAAKKPGGPQQHRSGSRHR"
     gene            complement(4143..5384)
                     /locus_tag="PLN02_00730"
     CDS             complement(4143..5384)
                     /locus_tag="PLN02_00730"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOI50875.1"
                     /translation="MKHTVPVDRLQPGVFISITGVGWLDHPFLLNRFRLTSHDQIKVL
                     RSLGLKEIEWDPARSTAQPLPEQKALPPEEDFSAAVMASMIHDKRERTERVRAQREGL
                     ARCERLYEQEANGVHDILRDLGSRPAESYGKAKNTVGRMVGSLIGSQSVAIHLVNMKV
                     KEPGMAQHAMNVAVLSLLLGRALGLEEEEMRWLGLGAMFHDIGKLEVPARVLRNSQRT
                     HAEEQFYQAHVGYGIKHVSVIPELPVGVRNAIACHHERWDGSGFPNRLQAGRIPRLAR
                     IVAIANRYDNLCNPFDLSTARTPGEAVAHMFKREGSHYDPELLQLFVKSVGVYPPGCF
                     VALNDGSVGLVVETNSSDLLHPLVMLHDPGVPRSEALLVDLREADVKIDSVLNPAKLP
                     VEVVEYLAPRGRVDYYIEGLH"
     gene            complement(5381..6568)
                     /locus_tag="PLN02_00735"
     CDS             complement(5381..6568)
                     /locus_tag="PLN02_00735"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HOI50876.1"
                     /translation="MSESSNYQPETLAVRAGQQRSQFGEHAEALYLTSSFVFESAAQA
                     ARRFSGEEEGNVYARFSNPTVSMFEERLAALEGAEDCVATASGMSAIMALVLAHLKQG
                     DHIVASNSLFGATVQLFSNILARTGITTSFVPATDPAAWAAAIRPETRLFFLETPSNP
                     LTEIADIRALAAIAHERGILVAVDNCFCTPILQKPLELGADLVVHSATKYIDGQGRVL
                     GGAVCGPKKLTEEVFKYLRTAGPTLSAFNAWVLLKGLETLQLRVEAQSANAARLAAWL
                     EAHPKVARVYYPGLASHPQHALAKTQQKSGGAIVSFEVRGGRPEAWGVVDHCRLLSIT
                     ANLGDVKTTITHPASTTHGRISPEARAAAGIGEGLLRIAVGLEAQADLQADLERGLSL
                     IGA"
     gene            complement(6571..>7515)
                     /locus_tag="PLN02_00740"
     CDS             complement(6571..>7515)
                     /locus_tag="PLN02_00740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOI50877.1"
                     /translation="ETAAGMEYLISSESVALDTLGFQMVRDIEPGEAVFIDLERNLHS
                     RQCAQQPMYAPCIFEYVYLARPDTVIDGVSVYEARLAMGELLAEKVKKVIPVDEIDVV
                     IPIPDSSRPSAMQLAQALGIPFREGFVKNRYVGRTFIMPGQAMRRKSVRQKLNTCGQE
                     FKGKRVLLVDDSIVRGTTSREIVEMARAAGAVKVYFASAAPPVRYPNVYGIDMPTRAE
                     LIATGRTDAQIAAEIGADALVYQDIEALKQSVTKLRPDLTVFDASCFDGCYVTGDIDE
                     YYLDAVEGKRGGKAGKNDEDGDGKASQQLVLGLAEGQD"
BASE COUNT         1170 a         2490 c         2550 g         1305 t
ORIGIN      
        1 ccaccttcgg cgtcggcggc gaactcgacg agcgcgaatg gaagagcgtc ttccgccagc
       61 tcgccgccgc cggcctggtc catgtcgaca tgaccgagca cggcgccctg caactgaccg
      121 aagcagcacg cgaggtactc aagggccggc gcagcgtgca attgcgccgg ccaaccagga
      181 aacgcagcgc gccgagcaag accagccgcg ccccagtggt cgacctggcg ccggccgacg
      241 ccgcgctgtt ccagcgcctg cgccagtggc gcgccgacac cgcgcgcgaa cagggcgtgc
      301 cggcctacgt catcctgcac gacaagaccc tgcgcgaact cgccgaatgc cgcccggtca
      361 gccatggcct gctcgccggg atcaccggca tgggcagcgc caagatcgag cactacggcg
      421 aggaactgct gcaactgatc cgcgaggaag cccgccatga agcatcctga ccgtctgacc
      481 gccatcctcg gcagcatcgt catcgccgcc ctgatcgtct ccggcatcgc cccctacgac
      541 cgcgccacct ggctgatgga ggtggcgccg gtgctgctcg ccctgccgtt gctctggctg
      601 acccggcgca gctttccgct caccgcgctg ctctacgtgc tgatcgccat ccacgccctg
      661 gtgctgatcg gcggcggcgc ctacacctac gcccgggtac cgttcggctt ctggctgcag
      721 gacctgctcg gcacgacgcg caatccctac gacaaggtcg gccacttcct gcagggtttc
      781 gtgccggcac tggtcgcccg cgaaatcctg ctgcgcgacg actgggtgcg cggcgcgaaa
      841 atgaccgctt tcctctgcat ctgcatcgcg ctggcgatca gtgcctgcta cgagctgatc
      901 gaatgggcgg cggcgctggc catgggccag ggcgccgacg aattcctcgg cacccagggc
      961 gacccctggg acacccagtc cgacatgttc ctcgcgctgc tcggcgcgat cgccgcgctg
     1021 ctcctgctct ccggctggca caaccgccag ttgcaccggc tgcggcgcgg ctgagcggct
     1081 gcctcagagg cagagcgcgt ccgggtcggc gcgcatcagg cgcttgttca tcttgccgac
     1141 gctggtcttg accagcgtct tgacgaagcg cacctgcagc agcacgccgt aacgcgaaac
     1201 gccggccgcc tcgatggcat gcgcggcata gttgcgcaag gcgtgctcgc tgacctggcc
     1261 gacgaactcc ggcttcaaca gcaccagcgc cagcggccgc tcgccccact tgtcgtcggc
     1321 cacgccgatc actgccacct cctgcaccgc ctcgtgcttg gcgacgatgt cctcgagttg
     1381 cagcgacgag gtccactcgc cgccggtctt gatcacgtcc ttgatccggt cggtgacctt
     1441 gagatagccg gcggcatcga tgttgccgat gtcggcggta tgcagatagc cgcccgccca
     1501 cagttcctcg gacgcctcgg gcgcatgcag gtagccctgg gtcagccagg gcgagcggac
     1561 gaccacctca ccggtggcct tgccgtcgtg ggcgacgtcg cgcatctgcg catcgacgat
     1621 gcgcaggtcg accagcggca aggggcggcc ggtcttggca cggatcaccg cctgctcggc
     1681 cggcgacttt tccaggtcgg cgctcgccag gtgggccagc gtcagcaccg ggcaggtttc
     1741 cgacatgccg tagccggtga acaggtcgat gccgcgagcc agcgcggcgc cggccatggc
     1801 ttccggcatc gccgcaccgc cgatgaccag cttccacccg gagaaatcgg ttttcgccgc
     1861 cgccggatgg ccgaacacca tgtgcaggat ggttggcacg caatgtgaaa agctgacccc
     1921 ctcctcctcg accaggcggc agatcacatc cggctggtag cggccgggat agacctgctt
     1981 gagcccgagc atcgtcgcca ggtaggggaa gccccaggcg tggacgtgga acatcggcgt
     2041 cagcggcatg tacacgtctt cgcggtgcag gcgcccctgg ccggccgcgt tgccggtgcc
     2101 gctggcgatg ccgagcgtgt gcaggaccag ttgccggtgg ctgaaataga cgcccttcgg
     2161 gttgccggtc gtgccggtgg tgtagaaggt cgtcgcctgc gcgttctcgt cgaaatctgg
     2221 aaagtcgtaa ttggcttccg cggcggccag catcgcctcg tactcgccgg cggcgaccac
     2281 cggcgacgcc ggcagcaccg cctcgtcgct gatcaagatg aaggcacgca cgctttccag
     2341 gcggccggcg atctcggcga ggatggggaa gaactcgcta ttgaccatga ccacgtcagc
     2401 ccgcgcgtgg ttcagcgtgt acagaatctg ttccggactg aggcggacat tgaccgtctg
     2461 cagcacggcg cccatcatcg gcaccgcgaa gaagctctcc aggtaacgat gactgtccca
     2521 gtccatcatc gccaccgtat ggccggcctc gacgccgagc gaggagagca cgttggccag
     2581 gcggccgata cgctggcgca gctcgcggta ggtgtagcgc agccggccct ggtaggtgat
     2641 ttcctggtct ggcgccgtcg ccagcggcgt cagcagcaac tgcttgatca gcagcggata
     2701 ttggtaggcc gacggcgtcg ccccgacgat ttttaccggc atctggttct ctccctgaaa
     2761 cactgttctt taccgctgta tttcagggat tttacgcctg ccggcggcgc cccactcagc
     2821 ggtggcggct accgctgcga tgttgttgcg gcccaccggg ttttttcgcg gctttcgccg
     2881 gtgcccgcgc cggctgcgcc gaacgtccgc cgcgcggcgg ctgcggcggc cgcggcgcgg
     2941 catgcgccgc cggctcgaaa ccttcgatga cgacgcgctc gagattgcgc ttgatcagct
     3001 tctcgatgtc cttcagttgc ggccgctcgt cgtggcagac cagcgagatc gcctcgcctt
     3061 ccatgccggc gcggccggta cggccgatgc ggtgcacgta gtcctcgggc acgttgggca
     3121 gttcgtagtt gatcacgtag ggcaaggcgt cgatgtcgat gccgcgcgcc gcgatgtcgg
     3181 tggcgaccag cgccgccagc ttgccttcct tgaattcgcc gaggacgcgg gtgcgcgcgc
     3241 cctgcgactt gtcgccgtgg atcgccgccg ccttgatgcc ggccttgtcc agcgcccgcg
     3301 ccagggcatc ggcgccatgc ttggtgcggg cgaagaccag tacctgatgc aggctgcgcg
     3361 tctcgaacag gtgcttgagg aggtccttct tctgctcgcg gttgcattcg atgacccgct
     3421 gcgccaccgt ttccgccgcc gtgttgcgcg gcgccacatc gaccgacacc ggctggtgca
     3481 gcaggccgcc cgccagttcg cggatttccg gcgagaaggt ggcggagaag agcaggttct
     3541 gccgctgctt gggcagcagc gcgatgatct tgcggatgtc gcggatgaag cccatgtcga
     3601 gcatgcggtc ggcttcgtcg aggacgaaga tctcgatgtg gccgaggtcg acggtcttct
     3661 gctggacgtg gtcgaggagc cggcccggcg tcgccaccag gatgtcgaca ccgcggcgca
     3721 agccggcgat ctgcgggttg atgccgacac cgccgaaaac ggccagcgag ttgatcggca
     3781 ggtgcttgcc gtaggtgcgc acgctttcct ccacctggat cgccagttcg cgggtcggcg
     3841 tcagcaccag cacgcgcggc gtgcgggcga cgcggctgag acggtcgttg gcgccggccg
     3901 acagacgctg caggatgggt agcgtgaagc cggcggtctt gccggtgccg gtctgcgccg
     3961 tggccatcag gtcgtggccg gcgagcacgg cgggaatcgc ctgctgctgg atcggcgtcg
     4021 gggtgtcgta gccctgttcg gcaacggcgc gttgaatgtc ggcgctaagg ccgagttcgg
     4081 aaaactgcat gggatgactc cggaaaaatg ccttcgggcg ccaacggcgc ccaggctgaa
     4141 ggtcagtgca gaccttcgat gtaatagtcg acgcggccgc gcggcgccag gtattcgacg
     4201 acctcgaccg gcagcttggc cggattgagg acgctatcga tcttcacgtc ggcctcgcgc
     4261 aggtcgacca gcagggcctc gctgcgcggc acgccggggt cgtgcagcat gaccaggggg
     4321 tgcagcaggt cgctgctgtt ggtctcgacg accagaccga cgctgccgtc gttgagggcg
     4381 acgaagcagc cgggcggata gacgccgacc gacttgacga agagttgcag cagctcgggg
     4441 tcgtaatgcg agccttcgcg cttgaacatg tgcgccaccg cctcgccggg agtacgcgcg
     4501 gtgctcaggt cgaacgggtt gcacaggttg tcgtagcggt tggcgatggc gacgatccgc
     4561 gccaaccgcg gaatgcggcc agcctgcagg cgattgggga agccgctgcc gtcccagcgc
     4621 tcatggtgac aggcgatggc gttgcgcacg ccgaccggca gttcgggaat caccgagacg
     4681 tgcttgatgc cgtagccgac gtgcgcctgg tagaactgtt cctcagcgtg cgtgcgctgg
     4741 ctgttgcgca acacgcgggc cggcacctcg agcttgccga tgtcatggaa catcgcgccc
     4801 aggccgagcc agcgcatttc ctcttcctcc agcccgagcg cccggccgag caggagcgac
     4861 aatacggcaa cgttcatcgc gtgctgggcc atgcccggtt ccttgacctt catgttgacc
     4921 aggtggatgg cgacactctg gctgccgatc aggctgccga ccatgcggcc gacggtgttc
     4981 ttggccttgc cgtaggattc ggcgggacgg ctgccgaggt cgcgcaggat gtcgtgaacg
     5041 ccgttggcct cctgctcgta cagccgctcg caacgggcca gcccttcgcg ctgggcgcgc
     5101 acgcgctcgg tgcgttcgcg cttgtcgtgg atcatcgagg ccatcaccgc cgctgagaaa
     5161 tcctcttccg gcggcaaggc cttctgttcc ggtagcggct gcgccgtact gcgcgccggg
     5221 tcccattcga tttccttgag ccccaggctg cgcaggacct tgatctggtc gtggctagtc
     5281 aggcgaaagc ggttgagcag gaacgggtgg tcgagccagc ccaccccggt gatcgagatg
     5341 aagacgccag gctgcagccg atcgaccggg accgtgtgtt tcatgcgccg atcagggaga
     5401 ggccgcgctc gaggtcggcc tgcaggtcgg cctgcgcttc cagaccgaca gcaatgcgca
     5461 gcaggccctc gccgatcccg gccgccgccc gcgcttccgg ggaaatccgg ccgtgcgtgg
     5521 tggacgccgg gtgagtgatg gtggtcttga cgtcgccgag gttggcggtg atcgacaaca
     5581 ggcggcagtg gtcgacgacg ccccaggctt ccggacgacc gccgcgcact tcgaaggaga
     5641 cgatggcgcc gcccgacttc tgctgcgtct tggccagcgc gtgctgcgga tgcgaggcca
     5701 gcccggggta ataaacgcgc gccaccttgg gatgcgcttc cagccaggcg gccagcctgg
     5761 cggcgttggc ggattgcgcc tcgacgcgca actgcagcgt ctccagcccc ttgagcagga
     5821 cccaggcatt gaatgccgac agggtcggcc cggcggtgcg taggtacttg aagacttcct
     5881 cagtcagctt tttcggaccg cagacggcgc cgccgagcac ccggccctgg ccgtcgatgt
     5941 acttggtcgc cgaatggacg accagatcgg cgcccagttc cagcggtttt tgcaggatcg
     6001 gcgtgcagaa gcagttatcg accgcgacca ggatgccgcg ttcgtgggcg atcgccgcca
     6061 gcgcccggat gtcggcgatt tcggtgagcg ggttggacgg cgtttcgagg aagaacaacc
     6121 tggtttccgg gcggatcgcc gccgcccagg ccgcagggtc ggtggccggc acgaagctgg
     6181 tggtgatgcc ggtacgcgcc aggatgttgg aaaacaactg gacggtggcg ccgaacaggc
     6241 tgttcgaggc gacgatgtgg tcgccctgct tgaggtgggc aagcaccagc gccatgatcg
     6301 ccgacatgcc gctggcggtg gcgacgcaat cctcggcgcc ttccagggcg gcgaggcgtt
     6361 cctcgaacat gctcaccgtc gggttggaga aacgggcgta gacgttgccc tcttcctcgc
     6421 cggaaaagcg ccgagcagcc tgcgctgcgc tctcgaagac gaagctggag gtcaggtaga
     6481 gcgcttcggc gtgctcgccg aactggctac gctgctgccc ggcccggacg gccagggttt
     6541 ccggctggta atttgaggac tcgctcatgc tcagtcctgc ccctcggcca gtcccagtac
     6601 cagttgctgc gaggccttgc cgtcgccgtc ctcgtcgttc ttgccggcct tgccgccacg
     6661 cttgccttcg accgcgtcga ggtaatactc gtcgatgtca ccggtcacgt agcaaccatc
     6721 gaaacaggaa gcgtcgaaca ccgtcaggtc cggacgcagt ttggtcaccg attgcttgag
     6781 cgcttcgatg tcctggtaaa ccagcgcgtc ggcgccgatt tcggcggcga tctgagcgtc
     6841 ggtgcggcca gtggcgatca gttcggcacg ggtcggcatg tcgatgccgt acacgttcgg
     6901 atagcgcacc ggcggcgccg ccgacgcgaa gtagaccttg accgcgccag cggcgcgggc
     6961 catctcgacg atctcgcgac tggtcgtgcc gcggacgatg gagtcgtcga ccaagagcac
     7021 ccgcttgccc ttgaattcct ggccacaggt attgagcttc tggcgcaccg atttccgccg
     7081 catcgcctgg ccgggcatga tgaaggtgcg gccgacatag cggttcttga cgaagccttc
     7141 gcggaacggg atgcccagcg cctgcgccag ttgcatcgcc gacgggcggc tggagtcggg
     7201 aatcgggatg acgacgtcga tctcgtccac cggaatcact tttttaacct tctcggccag
     7261 cagttcgccc atcgccagcc gcgcctcata gaccgaaacg ccgtcgatca cggtatccgg
     7321 ccgggcgaga tagacgtatt cgaagatgca cggcgcgtac atcggctgct gcgcgcactg
     7381 gcggctgtgc aagttgcgtt cgaggtcgat gaacaccgcc tcgcccggct cgatgtcgcg
     7441 caccatctgg aagccgaggg tgtcgagggc gaccgattcc gaggaaatca ggtactccat
     7501 gccggcggcg gtctc
//
LOCUS       DAOSDX010000017         7501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3608_length_7501_cov_3.205077, whole genome shotgun sequence.
ACCESSION   DAOSDX010000017 DAOSDX010000000
VERSION     DAOSDX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3608_length_7501_cov_3.205077"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLN02_00745"
     CDS             <1..215
                     /locus_tag="PLN02_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HOI50878.1"
                     /translation="LINYLHFSASGSDTLIQISSHGAYTGSNHATATDQTILLQGVAL
                     DSLAGAGAGDAQIISELLKANLKVDL"
     gene            complement(270..1028)
                     /locus_tag="PLN02_00750"
     CDS             complement(270..1028)
                     /locus_tag="PLN02_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOI50879.1"
                     /translation="MIDTHCHLDAAEFDADREAVLAAAQAAGVSRVVVPGVAFAAFAR
                     QRRIVADSPLCVAAYGIHPLYVDAAGDADLEHLASWVDAERPLAIGEIGLDFFVAGSD
                     IERQTRFFVSQLKLARALDLPVILHVRRAVDAVLRELRRVRVRGGIAHAFNGSPQQAA
                     EFIKLGFKLGFGGAASYSGSTRIRALAAALPAEAIVLETDAPDIPPAWLNRGRNTPAE
                     LPNIAREIAGLRQMPVTTLIAQADKNAQAVLGVF"
     gene            complement(1025..2281)
                     /locus_tag="PLN02_00755"
     CDS             complement(1025..2281)
                     /locus_tag="PLN02_00755"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HOI50880.1"
                     /translation="MRILVLGAGVVGTASAWYLHAAGHQVTVVDRQAAAGLETSFANG
                     GQISVSHAEPWANPHVLPRLLKWLGRPDAPLRWHWRADPGQWLWGLRFLRQCLPGRTQ
                     ANIAAILALALYSRGQLQRLREELGLEYEQLQRGILHIYTDQDELAAAMSAARLMARF
                     GLDREMLDVRACVALEPALAGAEKLLVGGDYTPSDESGDAHRFTQALAARAAAAGVVF
                     RHEQSIERIASAGGRVAGVVVRDAQGESSLLAADAYVVALGSYSPLLLRPLGIRLAIC
                     PAKGYSATLPLADGAVAPTVSLTDDEHKLVFSRFGNRLRVAGTAEFAGYDLALDELRC
                     QALMRRTRQLFPDLRLAGEPQFWCGLRPATPSNVPYLGRSRLPNLWLNTGHGTLGWTM
                     ACGSAAALSDLIDGRRPAPDFPFLRG"
     gene            complement(2278..4431)
                     /locus_tag="PLN02_00760"
     CDS             complement(2278..4431)
                     /locus_tag="PLN02_00760"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOI50881.1"
                     /translation="MEPAPQHRPPVVGDEVYRQFIARLDYLPPSDVARIEAAYAYAEQ
                     AHAHQKRMSGEPYITHPLAVASAVVEWCMDADAICAALLHDTMEDTGATKQELAEKFG
                     KEVAEMVDGLSKLDKMEFASYKEAQAENFRKMLMAMARDLRVVLIKLADRQHNIRTMS
                     AMRADKRVRIARETLEIYAPIALRLGLSRLYRELQDTAFRLIHPHRAEVLAKALKAAK
                     GNRRELLTRIQESVCDKLAHAGLEAEVFGREKSLYSIFCKMKEKRLSFSQVLDIYGFR
                     VVVDKVASCYLALGALHSLYKPVPGKFKDYIAIPKANGYQSLHTTLIGPYGTPVEVQI
                     RTREMHHVAQDGVASHWLYKDGSESSAELQIKTHKWLQSLLESQTGNSAEFFEDVKVD
                     LFPDEVYVFTPKGKIMALPHGATAVDFAYAVHTDVGNHCSAARINHELAPLRTELKNG
                     DLVEIITSPDASPNPVWLNYVKTGRARSKIRHFLRTTQNVESARLGERLLRQELLSLG
                     VVPISIPTAAWEALVKAGGQQSIDEVYADIGLGKRLPSVVARRLLAREGMAAAVPGGL
                     TLAIHGNEGMAIQLAPCCQPIPGDPIIGSIRKGSGMIIHTHDCPAIRKSRSSEPQKWI
                     DVEWEPEEGKLFDLRIRVEVKNKRGVLAQVAAAISEAASNIEHVSMEADPERLFTTLH
                     FTIQIANRKHLAAVMRGIRHLPEVSRIAREKGGEA"
     gene            complement(4454..4660)
                     /gene="rpoZ"
                     /locus_tag="PLN02_00765"
     CDS             complement(4454..4660)
                     /gene="rpoZ"
                     /locus_tag="PLN02_00765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOI50882.1"
                     /translation="MARVTVDDCLKRIPNRFQMTLAAAHRARQLANGATPLVDAEKDK
                     PTVIALREMAIGKVGLEVLNRGQA"
     gene            complement(4684..5292)
                     /gene="gmk"
                     /locus_tag="PLN02_00770"
     CDS             complement(4684..5292)
                     /gene="gmk"
                     /locus_tag="PLN02_00770"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOI50883.1"
                     /translation="MAGDLYIIAAPSGAGKTTLVRMLLEREPAIRLSISHTTRSPRAG
                     EADGREYHFVAVDAFRAMIARDEFLEWAEVHGNFYGTSKQWIADQLAAGRDVLLEIDW
                     QGAQQVRRLFPEAIGVFILPPSLDELTRRLTGRGTDAPDVIERRLAAAEAEMRHVGEF
                     DYVIINDQLEQALDDLRAIVRVPRLQPGRQRLRHPALFARLM"
     gene            complement(5304..6170)
                     /locus_tag="PLN02_00775"
     CDS             complement(5304..6170)
                     /locus_tag="PLN02_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOI50884.1"
                     /translation="MICSMTGYAVKTRDLERGTLQLELKSVNSRYLDFHFRVAEDLRS
                     MELPLRELLASRLSRGKVECRLSFNAAVARGEQLTLNDELLAALRGLADQVRTAMPEA
                     APLSVNEVLRWPGMFGDQEIDFAALGPVVLALAREALDDFTATRAREGEKLAAMIVDR
                     VNAMRAIVRDVAPRIPEAQAVFTDKLRQRLQEALGNASDDRILQEVAVFATRIDVAEE
                     LSRLTAHLDEVERVLKAGGASGKRLDFLMQELNREANTLASKSAITEVSQAAMELKLL
                     IEQMREQIQNLE"
     gene            6248..7195
                     /locus_tag="PLN02_00780"
     CDS             6248..7195
                     /locus_tag="PLN02_00780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOI50885.1"
                     /translation="MAQQANHPLPGGHQLEEYTIERQLSLGGFSIVYLARDPDNNQVA
                     IKEYLPNSLALRAKGDICPVITEDHLGAFRYGMKCFFEEGRALARLSHPNVIRVLNFF
                     RANETVYMVMEYEHGRTLQEFIQKHQGHISERFIRGVFTRMLNGLREVHSHKLLHLDL
                     KPSNIYLRSDNTPVLIDFGAARQTLVSDQPMLKPMYTPGFASPEHYGNRQDLGPWSDI
                     YSVGASMYACLDGAAPQAADERLRKDTLTPAMVRWEGQYSDRLLEIIDWCLSLNHLYR
                     PQSVFALQKALVADTHPPHARLESGWIGRFVDKFRNSPR"
     gene            7192..>7501
                     /locus_tag="PLN02_00785"
     CDS             7192..>7501
                     /locus_tag="PLN02_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOI50886.1"
                     /translation="MRFTIYQECRQGGRANNEDRSTYCYSRDALLMVIADGMGGHHHG
                     EIAAQLAVQTMAAAFQNEAKPLLDDPFDFLHRGMTHAHQAIRDYAVRHDLDDSPRTTC
                     V"
BASE COUNT         1343 a         2548 c         2358 g         1252 t
ORIGIN      
        1 acctgatcaa ctacctgcac ttctcggcca gcggcagcga caccctgatc cagatcagca
       61 gccacggcgc ctacaccggc agcaaccacg ccacggccac cgaccagacg atcctgctgc
      121 agggcgttgc gctcgacagc ctggccggcg ccggcgctgg cgacgcgcag atcatcagcg
      181 agttgctgaa ggccaacctc aaggtcgatc tctgatcgtc cgccggacag ccgtcctcaa
      241 cgcctgacca cgccccggcg cggcaggcct cagaaaacgc ccagcaccgc ctgggcgttt
      301 ttgtcggctt gagcgatcag cgtcgtcacc ggcatctggc gcaggccggc gatttcccgg
      361 gcgatgttcg gcagctcggc cggcgtgttg cggccgcgat tcagccaggc cggcggaata
      421 tccggcgcgt cggtttcgag gacgatcgct tccgccggca aggcggcggc cagcgcgcgg
      481 atgcgcgtcg atccgctgta gcttgccgcg ccgccaaagc cgagcttgaa accgagcttg
      541 atgaattccg cggcctgctg cgggctgccg ttgaaggcgt gggcgatgcc accacgcacg
      601 cgcacccgcc gcaactcccg cagcacggcg tccaccgcgc gccggacgtg gagaatcacc
      661 ggcaagtcga gcgcccgcgc cagcttgagc tggctgacga aaaaacgcgt ctggcgttcg
      721 atgtcgctgc cggcaacgaa gaaatcgagg ccgatctcac cgatcgccag cggccgttcg
      781 gcgtccaccc aggaagcgag atgctcgagg tcggcatctc cggcggcatc gacataaagc
      841 ggatggatgc catacgctgc cacgcacaac ggactatcgg cgacaatccg ccgctgtctg
      901 gcgaaggcgg cgaaggcgac gcccggaacg acgacccggc tcaccccggc ggcctgcgcc
      961 gccgccagta ccgcctcccg atcggcatcg aactccgccg cgtcgaggtg gcagtgggtg
     1021 tcgatcatcc gcgcaggaaa gggaaatcgg gtgccgggcg gcggccgtcg atcagatcgc
     1081 tcaaggccgc cgccgaacca caggccatcg tccagccaag cgtgccgtga ccggtgttca
     1141 gccacagatt gggcaagcgc gagcgcccga gatagggaac gttggacggc gtcgccgggc
     1201 gcagaccgca ccagaactgc ggctcgccag ccagacgcag gtcgggaaac aactgccggg
     1261 tgcgccgcat caaggcctgg caacgcagtt cgtcgagtgc cagatcgtaa cccgcgaact
     1321 cggcggtgcc ggcgacgcgc agacggttgc cgaaacggga aaagaccagc ttgtgctcgt
     1381 cgtcggtcag gctgacggtc ggcgccacgg caccgtcggc gagcggcagc gtcgccgaat
     1441 agcccttggc cggacagatc gccaggcgta tcccgagcgg ccgcaggagc aggggcgagt
     1501 agctgccgag ggcaacgaca taagcgtcgg cggcgagcaa ggacgattcg ccctgcgcat
     1561 cgcgcacgac aacgccggcg acacgaccgc cggcactggc gatccgctcg atgctctgtt
     1621 cgtgacggaa aacgacgccg gcagcggctg cccgggcagc cagcgcctgg gtgaaacgat
     1681 gggcatcacc ggattcgtcg gacggcgtgt agtcgccgcc gaccagcagt ttttccgccc
     1741 ccgccagcgc cggctcgagg gccacgcagg cccggacgtc aagcatctct cggtcgaggc
     1801 cgaaacgggc catcaggcgg gccgcgctca ttgccgccgc cagttcgtcc tgatcggtgt
     1861 agatatgcag gatgccgcgc tgcagctgct cgtattcgag ccccagttct tcgcgcaaac
     1921 gctgcaactg gccgcggctg tacaacgcca gcgccaggat cgccgcgatg ttggcctgcg
     1981 tccggccggg caggcactgg cgcaggaaac gcaaacccca cagccattgt cccgggtcgg
     2041 cgcgccaatg ccagcgcaag ggcgcatccg ggcgccccag ccatttcagc aagcgtggca
     2101 gaacatgtgg attggcccag ggttcggcgt gtgaaaccga tatctgccca ccgttggcaa
     2161 agctggtttc aaggccggcc gcggcttgcc ggtccacgac cgtgacctga tgaccggcgg
     2221 catgcagata ccaggcgctg gcggtaccga caacccccgc cccgaggacc agtatcctca
     2281 agcctcgccc cccttctccc gggcaatgcg gctgacttca ggcaaatgcc ggatgccgcg
     2341 catcaccgct gcgagatgct tgcggttggc aatctggatg gtgaaatgca gggtagtgaa
     2401 caagcgctcg ggatcggctt ccatcgacac atgttcgatg ttcgaagcgg cctctgaaat
     2461 ggcggcggca acctgcgcca gcacgccgcg cttgttcttc acctcgaccc ggatacgcag
     2521 gtcgaacagc ttgccttctt cgggctccca ctcgacatcg atccacttct gcggctcgct
     2581 cgaacgcgac ttgcggatcg ccgggcaatc gtgggtgtgg atgatcatgc cactgccctt
     2641 gcggatcgaa ccgatgatcg gatcgccggg aatcggctgg cagcatgggg caagctggat
     2701 cgccatgcct tcgttgccat ggatcgccag ggtcaatccg cccggtaccg ctgccgccat
     2761 gccctcgcgc gccagcaggc gacgggcgac gaccgagggc aggcgcttgc cgagcccgat
     2821 atcggcgtaa acctcgtcga tcgactgctg gccgcccgcc ttgaccagcg cctcccaggc
     2881 ggcggtcgga atcgaaatcg gcaccacgcc gagcgagagc aactcctgac gcagcaggcg
     2941 ctcgccgaga cgggccgatt cgacgttctg ggtcgtgcgc agaaaatggc ggatcttgct
     3001 gcgcgcgcgg ccggtcttga cgtagttgag ccacaccggg ttcggcgatg cgtcgggtga
     3061 ggtgatgatc tcgaccagat cgccgttctt cagctccgta cgcagcggcg ccagttcgtg
     3121 gttgatgcgg gccgccgaac aatggttacc gacgtcggta tgcaccgcgt aggcgaaatc
     3181 gaccgccgtc gccccatgag gcaaagccat gatcttgccc ttgggcgtga acacgtaaac
     3241 ctcgtccgga aagagatcga ccttgacgtc ctcgaagaac tcggccgaat tcccggtctg
     3301 cgactcgagc agcgactgca gccatttgtg cgtcttgatc tgcaactcgg cgctgctctc
     3361 gctgccatcc ttgtacagcc agtgcgaagc gacgccgtcc tgggcgacgt gatgcatctc
     3421 gcgggtacga atctggactt ccaccggcgt gccatagggg ccgatcagcg tcgtgtgcag
     3481 cgactggtag ccgttggcct tggggatcgc gatgtagtcc ttgaacttgc ccggcaccgg
     3541 cttgtacagg ctgtgcaggg cacccagcgc cagatagcag ctcgccacct tgtcgacgac
     3601 gacgcggaag ccgtagatgt cgagcacctg cgagaaggaa agccgctttt ccttcatctt
     3661 gcagaagatc gaatagagac ttttctcgcg cccgaagact tccgcttcca gcccggcatg
     3721 cgccagcttg tcgcagacgc tttcctggat ccgggtcagc agttcgcgcc ggttgccctt
     3781 cgccgccttc agcgccttcg ccaggacctc ggcacgatgc gggtggatca ggcggaaagc
     3841 ggtgtcctgc aattcccggt agagccggct caagcccagg cgcaaggcga tcggcgcgta
     3901 gatttcgagg gtttcccggg cgatgcgcac gcgcttgtcg gcgcgcatcg ccgacatcgt
     3961 ccggatgttg tgctggcggt cggcgagctt gatcagcacg acgcgcaggt cgcgcgccat
     4021 cgccatcagc atcttgcgga agttttccgc ctgcgcctcc ttgtacgagg cgaattccat
     4081 cttgtccagc ttggacaggc cgtcgaccat ttcggcgact tccttgccga acttctcggc
     4141 caactcctgc ttggtcgccc cggtgtcctc catcgtgtcg tgcaggaggg cggcacagat
     4201 cgcatcggcg tccatgcacc attcgacgac ggcgctggcc accgccagcg gatgggtgat
     4261 gtagggttcg cccgacatgc gcttctggtg cgcatgcgcc tgctcggcgt aggcgtaggc
     4321 cgcctcgatg cgtgctacat cagacggcgg caggtagtcg aggcgagcga tgaactggcg
     4381 atagacctcg tctccgacaa ccggtgggcg gtgttgaggc gcaggctcca tccgggttca
     4441 ccgccccgcg cggtcaggcc tgaccgcggt tgagcacttc caggccgacc ttgccgatgg
     4501 ccatctcgcg cagggcgatg acggtcggct tgtccttctc ggcatcgacc agcggcgtcg
     4561 cgccgttggc aagctggcgg gcccggtggg cggcggccag ggtcatctgg aagcggttgg
     4621 gaatcctttt caggcaatca tcgacggtaa cgcgagccat gtctaacctc agttcaaatc
     4681 cgtttacatc agtcgggcga agagcgcggg atggcgcaat cgctgccgac cgggctgcag
     4741 gcggggaacg cgcacgatgg cgcgcagatc gtccaatgcc tgctccaatt ggtcgttaat
     4801 aataacatag tcgaattccc cgacatggcg catctccgcc tcagccgcgg cgagacggcg
     4861 ctcgatgaca tccggcgcat cggtaccgcg cccggtcagg cgacgggtca gctcatcgag
     4921 cgaaggcggc aggatgaata ccccgatcgc ttccggaaac agccgacgca cctgctgcgc
     4981 gccctgccag tcgatttcga gcaggacatc gcgaccggcg gcaagctggt cggcgatcca
     5041 ctgcttcgac gtaccgtaga agttgccgtg gacttccgcc cactccagga attcgtcacg
     5101 ggcgatcatc gcccggaagg cgtcgacggc gacgaaatgg tattcgcgtc cgtccgcctc
     5161 gcccgctcgc gggctgcgcg tggtgtggga aatcgacagg cggatcgccg gctcgcgttc
     5221 gagcagcatc ctgaccagcg tcgtcttgcc ggcgcccgag ggcgccgcaa tgatgtaaag
     5281 atcgcccgcc atctaccctc cgctcattcc aggttctgga tctgctcgcg catctgctcg
     5341 atcagcagct tgagctccat cgccgcctgc gaaacctcgg tgatcgccga cttcgatgcc
     5401 agcgtgttgg cctcgcggtt gagttcctgc atcaggaaat cgaggcgctt gccgctggcg
     5461 ccgccggcct tcagcacccg ctcgacctcg tcgaggtggg cagtcaggcg cgacagttcc
     5521 tcggcgacgt cgatgcgcgt cgcgaacacc gccacttcct gcaggatgcg gtcgtcgctg
     5581 gcgttgccca gcgcttcctg cagacgttgc cgcagcttgt cggtgaacac ggcctgcgcc
     5641 tcgggaatgc gcggcgcgac atcgcggacg atggcgcgca tggcattgac ccggtcgacg
     5701 atcatcgccg ccagcttttc gccttcgcgg gcgcgggttg ccgtgaaatc gtcgagcgct
     5761 tcgcgggcca gcgccaggac gaccgggccg agcgcggcaa aatcgatttc ctggtcgccg
     5821 aacatgcccg gccagcgcag cacctcattg accgacagcg gcgccgcttc cggcatcgcc
     5881 gtgcgcacct ggtcggcgag gccgcgcagg gcggcgagca attcgtcgtt gagcgtcagt
     5941 tgttcgccgc gcgccaccgc cgcgttgaac gacagccggc attcgacctt gccccgcgac
     6001 agacggctgg cgagcagttc gcgcagcggc aattccatgc tgcgcaaatc ttccgcaacc
     6061 cggaaatgaa aatccagata gcgggaattg acgcttttta gttccaattg cagcgttccc
     6121 cgctcaagat cgcgggtttt cactgcatat ccggtcatgc tgcaaatcat cggggctcgc
     6181 tctccagctt tacaggtcgg acaacacgcc cgacaatgcc tgcgattgca tgataaccgc
     6241 gagatcgatg gcgcaacaag ccaaccaccc gctgcctggc ggccaccagc tggaggagta
     6301 caccatagaa cgccagctgt cgctgggcgg gttttccatc gtctatctgg cccgcgaccc
     6361 cgacaacaac caggtcgcga tcaaggaata cctgcccaac agcctggccc tgcgcgccaa
     6421 gggcgacatc tgcccggtca tcaccgaaga ccacctcggc gccttccgtt acgggatgaa
     6481 gtgcttcttc gaggaaggcc gcgccctggc ccgcttgtcg catccgaacg tgatccgtgt
     6541 gctgaacttc ttccgcgcca acgaaaccgt ttacatggtc atggaatacg aacatggccg
     6601 cacgctgcag gaattcatcc agaaacacca ggggcatatt tccgaacgct tcatccgcgg
     6661 cgtgttcacc cgcatgctca acggcctgcg cgaagttcat tcgcacaagc tgctgcacct
     6721 cgacctcaag ccctcgaaca tctacctgcg cagcgacaac acgccggttc tgatcgactt
     6781 cggcgccgcc cgccagacgc tggtcagcga ccagcccatg ctcaagccga tgtacacccc
     6841 gggcttcgcc tcgccggagc actacggcaa ccgccaggat ctcggcccgt ggagcgacat
     6901 ctacagcgtc ggcgcctcga tgtacgcctg cctcgacggc gccgcgccgc aggccgccga
     6961 cgagcgcctg cgcaaggaca cgctgacgcc ggccatggtg cgctgggaag gccagtattc
     7021 cgaccgcctg ctcgaaatca tcgactggtg cctcagcctc aaccacctct accgtccgca
     7081 aagcgtcttc gcgctgcaga aggcgctggt cgccgacacc catccgccgc acgcccgcct
     7141 ggaaagcggc tggatcggcc gcttcgtcga caaattcagg aattcgccgc gatgagattc
     7201 accatctatc aggaatgccg ccagggcgga cgggccaaca acgaagaccg cagcacctac
     7261 tgctattcgc gcgacgccct gttgatggtg atcgccgacg gcatgggcgg ccatcaccac
     7321 ggcgagatcg ccgcccaact ggcggtgcag accatggccg cagcgttcca gaacgaagcc
     7381 aaaccgctgc tcgacgaccc cttcgatttc ctgcaccggg gcatgaccca cgcccaccag
     7441 gcgatccgcg attacgccgt gcgccacgac ctcgacgact cgccgcgcac cacctgcgtc
     7501 g
//
LOCUS       DAOSDX010000018         7436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3660_length_7436_cov_3.468636, whole genome shotgun sequence.
ACCESSION   DAOSDX010000018 DAOSDX010000000
VERSION     DAOSDX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_7436_cov_3.468636"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLN02_00790"
     CDS             complement(<1..238)
                     /locus_tag="PLN02_00790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI50887.1"
                     /translation="MNKETTHFGFETVAEQEKARRVRSVFDSVASRYDLMNDLMSGGM
                     HRLWKAFTIERSGVREGSRVLDVAGGTGDLSLAFA"
     gene            complement(245..661)
                     /locus_tag="PLN02_00795"
     CDS             complement(245..661)
                     /locus_tag="PLN02_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOI50888.1"
                     /translation="MAGMEKDTPIPEAIRLHTKSRVLELVYGETVFRLPFEYLRVMTP
                     SAEARGHGPGQETLQVGKRDVELLAVEPSGTYALKLVFSDGHDSGLYTWDLLYNLGVH
                     QDELWAEYLRLLEAQGASRDSAGTPAAAARSCSHHH"
     gene            complement(718..2319)
                     /locus_tag="PLN02_00800"
     CDS             complement(718..2319)
                     /locus_tag="PLN02_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50889.1"
                     /translation="MSNEQSVQLSIVDLNDGMNYLAKLMLANPGTAERQLMDFLDALL
                     TAPPDPGILFALLEQTRAPLCFVEEEMARLYHNKAVPLSHDEESAFRAAVDAWRKMGK
                     AYALCARLEEPAVEAPGYKEMMATLLHRCIYYTGMVIIEHYRAHRELPPGIWLDLHGY
                     FETAEEWGIVFTPVSDPLESTTHATHCAAAYVALLLIDVASPYSHSVRNLNLIRRWAV
                     MWAPLVGVSPLESDLELPPYVVELMKDQPLHLSASVEGAASDVRRLDTARLGLQIDHA
                     INQLRMRYTPSQLGLGEETSSHVLKLLEQLVRPWSQSSSARRFRRFVSEGTARVATGF
                     TPMFFHVSGVPFEQPDSAAAYSRGDFEQLFTFRDRADPGQTLVIRPHADYPVEYWSVI
                     NHSANGFRLGRSGAGQRVQHGQLMAVCPHDGEHFLLGYATWLMQEESGGLVVGLATLP
                     GMPQGVSFRSLDVKSASSERYMAGFLLPALSAIGEEQSLVLPPGNYHAGRDLEVVIGE
                     EKRKLRLNHILLRQADFERISVVEL"
     gene            complement(2309..2746)
                     /locus_tag="PLN02_00805"
     CDS             complement(2309..2746)
                     /locus_tag="PLN02_00805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOI50890.1"
                     /translation="MGSACDLCGGPGGRLLWQSADCRVVRVVDPALPGFCRVIWNRHV
                     AEMTDLDAASQQTLMRVVFAVETAVRKCFAPDKVNLASFGNVVPHLHWHVIPRWRDDR
                     HFPEPIWGRQQRESTPARATVEDAMLTAIITGELAREENSHEQ"
     gene            complement(2858..6733)
                     /locus_tag="PLN02_00810"
     CDS             complement(2858..6733)
                     /locus_tag="PLN02_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOI50891.1"
                     /translation="MTARLREIPYNYTSFSDREIVIRLLGADNWAILDELRGERVTGR
                     SARMLYEVLGDIWVVQRNPYLEDDLLDNRERRDALVSALRHRLKEVEKRRTESESDDP
                     ERSAKVKRLVEAAHEAVNRFEARFGETIDLRRKVLKVLGKHTRRDNIAFDGLARVSHV
                     TDATDWRVEYPFVVLYPDTEEEIGHLVRGCIEAGLTIIPRGGGTGYTGGAVPLTPHSA
                     VINTEKLIDLGPVEELVLAGHETPYATIRTGAGVVTDRVSEAASAAGRVFAVDPTSAS
                     ASCIGGNIAMNAGGKKAVLWGTALDNLAWWKMVDPDGNWLEVERVNHNYGKIHEQENV
                     EFRVKRFDPSGKKALGEEVLVMPGAACRKTGLGKDVTDKFLGGIPGVQKEGTDGIIVA
                     ARWVLHKMPPVTRTVCLEFFGQVREAVPAIVEITDYFKPGGAGNAAGVQLAGLEHLDE
                     RYVKAVGYATKAKRHGRPKMVLIGDIVGHDEKAVMAAASEVVRMCNLRAAEGFIAVDA
                     ETRKKFWLDRSRTAAISRHTNAFKVNEDVVIPLPRMGEYCDGIERINIELSTQNKLAL
                     CDALSEFLKGELPLHSGDANLDKDLLIGDRRQAALDYVEAVRLRWEWLLENLDLPLAE
                     AESRFASYGVVAGELTNKAENPKLFHRLQDYSVRTSWKQELKARLAKIFDGTVYRPIL
                     ERIEAIHKEVLRGRVFVALHMHAGDGNVHTNIPVNSDNYAMLQTANKAVERIMHLARG
                     LDGVISGEHGIGITKLEFLTDDEIGPFRAYKQKVDPEGRFNKGKLMPGGDLGNAYTPS
                     FSLLGTESLIMEQSEIGKISDMVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSL
                     LVEAFLYEEQTRRGISLKHFDEFNDVADHCTVCHRCVKPCPVDIDFGDVSVAMRNFLR
                     KQEKKRFSPGTAASMLFLSAKDPATVKLLRGVMMNFGFKAQRLAHKAAKALHLVQETR
                     KHPPASLGAPSVKTQVIHFMNRPMPGNLPKRTSRALLDIENDEVIPVIRNPQKTTEDS
                     DAVFYFPGCGSERLFSQVGLATQAMLYDIGVTTVLPPGYLCCGYPQTASGNEDEGQKI
                     TTENRVLFHRVANTLNYLDIRTVIVSCGTCMDQLQKYHFEKIFPGCRLLDIHEYLMEK
                     GVKLEGVEGTRYMYHDPCHTPMKAYNPLAVTKALMGQEVPLNDRCCGDSGSFAYSRPD
                     IATQVKFRKQQEIEMGAEKLRGTGFQGEVKILTSCPACLQGLSRYYDDAGTQADYIVV
                     ELAKHLLGDNWMADYVGKANNGGIERVLL"
     gene            complement(6825..7244)
                     /locus_tag="PLN02_00815"
     CDS             complement(6825..7244)
                     /locus_tag="PLN02_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOI50892.1"
                     /translation="MLTGFDPGAGLWGLGLYSLLAATLLPGGSELALYAFLKYAPTQA
                     YPALLVATLGNTLGGLISWACGRFLPRWQRVERLPQVDRLRRWGAAALLFSWLPLIGD
                     ALCIAAGWLRLHWLPCALFMALGKFARYWLVVQAATN"
     gene            complement(7244..>7436)
                     /locus_tag="PLN02_00820"
     CDS             complement(7244..>7436)
                     /locus_tag="PLN02_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50893.1"
                     /translation="AASEISNAAREQGVTSQAIAQAVERVANLADSNSQQASALLGEV
                     RALENVARSLEEGAARFRT"
BASE COUNT         1391 a         2344 c         2341 g         1360 t
ORIGIN      
        1 tggcgaaagc cagcgacagg tcacccgtcc cgcctgcgac atcgagcacc cgcgaacctt
       61 cgcggacacc gctacgttcg atggtgaagg ccttccacag ccggtgcatg ccgcccgaca
      121 tcaggtcgtt catcagatcg taacggctgg ccaccgaatc gaagacgctg cgcacgcgac
      181 gcgccttttc ctgctcggcg acggtttcaa aaccgaaatg ggtcgtttcc ttgttcatgg
      241 gctctcagtg gtgatggctg caggatcgcg cggcggcagc gggagtgccg gcgctgtcgc
      301 gggaggcgcc ctgcgcctca agcaggcgga ggtactccgc ccacagttcg tcttgatgca
      361 cgccgaggtt atagaggaga tcccaggtgt acaaaccgct gtcgtggccg tcggaaaaca
      421 ccagcttcag cgcgtaggtt ccagagggtt cgaccgccag caattcgacg tcgcgcttgc
      481 cgacctgcag cgtttcctga cctggtccgt ggccacgcgc ttcagccgac ggcgtcatca
      541 cccgcaggta ttcgaagggc agacgaaaaa ccgtttcgcc atatacgagt tcgagcaccc
      601 ggcttttcgt atgcagacga atcgcctcgg ggatcggggt gtctttttcc atgccggcca
      661 tctgtggtct ccatgccagg gcggctagtt taccccgaag ctgccggcag cggcagccta
      721 cagttcgaca acgctgatcc gttcgaaatc ggcctggcgc aacaggatgt gattgaggcg
      781 cagcttgcgt ttctcttccc cgatgacgac ttccagatcg cggccggcgt ggtaattgcc
      841 cggcggcagg accagtgact gttcctcgcc gatcgccgat agtgccggca acaggaaacc
      901 cgccatgtaa cgttcgctac tggcgctctt gacatcaagc gaccggaagc tgaccccttg
      961 cggcatcccc ggcagagtag ccaagccgac taccagaccg cccgactctt cctgcatcag
     1021 ccaggtcgcg taaccgagca ggaaatgctc gccatcatga gggcagaccg ccatcagttg
     1081 tccgtgctgg acacgctgac cggcgccact gcggcccagc cggaaaccgt tggccgaatg
     1141 attgatcacc gaccagtatt cgaccggata gtcggcatgc gggcggatga ccagggtctg
     1201 acccggatcg gcccgatcgc ggaaagtgaa caattgctcg aaatcgcccc gggaataggc
     1261 agcggcggag tccggttgct cgaacggaac gccgctcaca tgaaagaaca ttggcgtgaa
     1321 ccccgtggcg acacgcgccg taccctcact gacgaaacgc cggaaacgac gggccgacga
     1381 agattgcgac cagggccgaa cgagttgttc gagcagcttc agtacatgac tgctggtctc
     1441 ctcgccgaga ccaagttgcg acggggtata gcgcatgcgc agctgattga tcgcatgatc
     1501 gatctgcagc cccaggcgcg ccgtatccag gcgccggaca tcgctcgccg ccccctcgac
     1561 cgaggcactc agatgcaaag gctgatcctt catcaactcg accacgtaag gcggcagttc
     1621 gagatcgctc tccagcgggc tgacgccgac cagcggcgcc cacatcaccg cccagcggcg
     1681 gatcaggttg aggttgcgca cgctgtggct gtaggggctg gccacgtcaa tcaacagcag
     1741 ggcaacgtaa gcggctgcac aatgggtcgc gtgcgtggtg ctttccagcg gatcgctgac
     1801 cggggtgaaa acaattcccc attcttcggc agtttcgaaa tagccgtgca ggtcgagcca
     1861 gatgccgggc ggcagttcac ggtgagcacg ataatgctcg atgatcacca tcccggtgta
     1921 atagatgcag cggtgcagca gggtcgccat catctccttg taaccgggcg cctccacggc
     1981 cggctcttcg agacgggcac acagcgcgta ggccttgccc atcttgcgcc aggcgtcgac
     2041 ggcggcgcgg aaagccgact cctcgtcgtg gctcagtggc actgccttgt tgtgatagag
     2101 acgggccatt tcctcttcga cgaagcagag cggcgcccgc gtctgttcga gcagggcgaa
     2161 aaggataccg gggtcgggcg gcgccgtcag cagcgcatcg aggaaatcca tcaactgccg
     2221 ttcggcggtt cccggattgg ccagcatcag cttggccagg tagttcatgc catcgttcag
     2281 atcgacgatc gacaactgca cgctctgctc attgctcatg gctattttcc tctcgcgcca
     2341 actcgccggt gatgatggcc gtcagcatgg cgtcctcgac ggtcgcgcga gccggcgtcg
     2401 actcgcgctg ctgccggccc cagatcggtt ccgggaaatg acgatcgtcg cgccagcggg
     2461 ggatgacatg ccaatgcaaa tgcggaacga cgttcccgaa gctggcgaga ttcaccttgt
     2521 ccggcgcaaa acacttgcgc accgccgtct cgacggcgaa gacgacgcgc atcagcgtct
     2581 gctgcgacgc cgcgtcgagg tcggtcattt cggcgacatg acggttccag atgacccggc
     2641 agaagccggg cagggcggga tcgaccacgc gcacgacgcg acagtccgcc gactgccaaa
     2701 gcagccgccc ccctggtccg ccacatagat cgcaagcgct tcccatcacc acatcgaatc
     2761 ccgtcgtttt gcataggatt gcattcgccg tcacttcctg caagtggcgg cgaaaaatag
     2821 cctgctcaag acggtcagcg gaagggaaaa gccgcgatca gagcaatacg cgctcgatac
     2881 cgccgttgtt ggccttgccg acgtaatccg ccatccagtt gtcgccgagc aagtgcttcg
     2941 ccagctcgac cacgatgtaa tcggcctgcg tgccggcatc gtcgtagtaa cgggaaagcc
     3001 cctgcagaca ggccggacac gaggtcagga tcttgacctc gccctggaag cccgtacccc
     3061 gcaacttctc cgcccccatc tcgatttcct gctgcttgcg gaacttcacc tgcgtcgcga
     3121 tatccggccg cgaataggcg aaggaacccg agtcgccgca acaacggtcg ttcagcggca
     3181 cctcctggcc catcagcgcc ttggtcacgg cgagcgggtt gtaggccttc atcggcgtat
     3241 ggcagggatc atggtacatg tagcgcgtcc cctcgacgcc ttcgagcttg acaccctttt
     3301 ccatcaggta ttcgtgaatg tcgagcaaac gacaaccggg gaagatcttc tcgaagtggt
     3361 atttctgcaa ctgatccatg caggttccac atgaaacgat caccgtcctg atatcgagat
     3421 aattcagcgt gttggcgacg cgatggaaaa gcacacggtt ctcggtggtg atcttctgac
     3481 cttcgtcctc gttgcccgaa gcggtctgcg ggtaaccaca acagaggtag cccggcggca
     3541 gcacggtggt gacgccgatg tcgtagagca tcgcctgtgt cgccagaccg acctgcgaga
     3601 acaagcgctc ggaaccgcaa ccggggaagt agaagacggc gtccgaatcc tcggtcgtct
     3661 tctgcgggtt gcggatcacc ggaatcacct cgtcgttctc gatgtcgagc aaggcgcgcg
     3721 aggtgcgttt gggcaggttg cctggcatcg gccggttcat gaagtggatc acctgcgtct
     3781 tcacgctggg cgcgccgagt gaggccggcg gatgcttgcg cgtttcctgg accagatgca
     3841 gcgccttggc cgccttgtgc gccagacgct gggccttgaa accgaaattc atcatgacgc
     3901 cgcgcagcaa cttcaccgtc gccgggtcct tggcgctgag gaagagcatc gaggcagccg
     3961 ttcctggcga gaaacgcttc ttctcctgct tgcgcaggaa gttgcgcatg gccacggaaa
     4021 catcgccgaa gtcgatatcg accgggcagg gtttgacaca acggtggcag accgtgcaat
     4081 ggtcggcgac atcgttgaac tcgtcgaagt gcttgagcga gatgccgcgc cgggtctgct
     4141 cctcgtacag gaaggcctcg accagcagcg aggtggcgag gatcttgttg cgcgggctgt
     4201 agaggaggtt ggcgcgcggc acgtgggtcg agcagaccgg cttgcacttg ccgcagcgca
     4261 ggcagtcctt gaccatgtcg gaaatcttac cgatctccga ctgctccatg atcagcgatt
     4321 cggtaccgag caggctgaac gacggcgtgt aggcattgcc cagatcgcca ccgggcatca
     4381 gcttgccctt gttgaagcgg ccttccgggt cgaccttctg cttgtaggca cggaacggcc
     4441 cgatttcgtc gtcagtcaaa aattccagct tggtgatgcc aatcccgtgc tcgccggaaa
     4501 tcacaccgtc gagcccgcgc gccaggtgca tgatgcgctc gaccgccttg ttggcggtct
     4561 gcagcatcgc gtaattgtcg gaattgaccg ggatgttggt atgcacgttg ccgtcgccag
     4621 cgtgcatatg cagggcgaca aagacgcggc cgcgcagcac ttccttgtgg atcgcctcga
     4681 tccgctcgag gatcggccga tagaccgtgc cgtcgaaaat cttggccaga cgcgccttca
     4741 actcctgctt ccacgaggtg cgcacggagt aatcctgcag gcgatggaag agcttcggat
     4801 tctcggcctt gttggtcagt tcaccggcca ccacgccgta ggaggcaaag cgcgattcgg
     4861 cttcggccag cggcaggtcg aggttttcca gcagccattc ccagcgcagg cggacggctt
     4921 cgacgtaatc gagcgcggct tgccggcggt cgccgatcag caaatccttg tcgagattgg
     4981 cgtcgccgga gtgcaacggc agttcgccct tgaggaactc ggacagggcg tcgcacagcg
     5041 ccagcttgtt ctgcgtcgac agttcgatgt tgatgcgctc gatgccgtcg cagtactcgc
     5101 ccatgcgcgg caacgggatg acgacatctt cgttgacctt gaaggcgttg gtgtggcgcg
     5161 atatggcggc ggtacgcgaa cggtcgagcc agaatttctt gcgtgtttcc gcgtcgaccg
     5221 cgatgaagcc ttcggcagca cgcaggttgc acatgcgtac gacttccgag gcggcggcca
     5281 tcaccgcctt ttcgtcgtga ccgacgatgt cgccgatcag caccatcttc ggccggccgt
     5341 ggcgcttggc cttggtcgcg taaccgaccg ccttcacata gcgctcgtcg aggtgttcga
     5401 ggccagccag ttgcacgccg gcggcgttgc cggcgccacc cggcttgaag taatcggtga
     5461 tctcgacgat cgccggcacc gcttcacgaa cctggccgaa gaattcgaga caaacggtgc
     5521 gcgtcaccgg cggcatcttg tgcagcaccc agcgcgcggc gacgatgatg ccgtcggtac
     5581 cttccttctg cacgccggga atgccgccga ggaacttgtc ggtgacgtcc ttgcccaggc
     5641 cggtcttgcg gcaggcggcg ccgggcatga ccagcacttc ctcgccgagc gccttcttgc
     5701 cggaagggtc gaagcgcttg acgcggaatt cgacgttttc ctgctcgtga atcttgccgt
     5761 aattgtgatt gacccgttcg acttccagcc agttgccgtc cggatcgacc atcttccacc
     5821 aggccagatt gtcgagcgcg gtaccccaca gcacggcctt cttgccgcca gcgttcatgg
     5881 cgatgttgcc gccgatgcag gacgccgaag ccgaggtcgg atcgaccgca aagacgcgac
     5941 cggccgccga ggcggcttcc gaaacgcggt cggtgaccac tccggcgccg gtgcggatcg
     6001 tcgcgtacgg ggtttcgtgg ccggccagca ccagttcctc gaccggaccg aggtcgatca
     6061 atttctcggt attgatcact gccgaatgcg gcgtcaacgg cacggcacca ccggtgtaac
     6121 cggtaccgcc gccgcgcggg atgatggtca ggccggcttc gatgcagcca cgcacaagat
     6181 ggccgatctc ctcctcggta tccgggtaga ggacgacgaa ggggtattcg acgcgccagt
     6241 cggtggcgtc ggtgacgtgc gaaacgcggg ccaggccatc gaaagcgatg ttgtcgcgac
     6301 gggtgtgctt gcccagcacc ttgagcacct tgcgccgcag gtcgatggtc tcgccgaaac
     6361 gcgcctcgaa acggttcact gcctcgtgcg ccgcctcgac cagacgcttg accttggccg
     6421 agcgctccgg atcgtcgctc tcgctttcgg tgcggcgctt ctcgacttcc ttcaggcgat
     6481 gacgcaaggc actgaccagg gcgtcgcggc gctcgcggtt gtcgagcaga tcgtcttcca
     6541 gataaggatt gcgctgcacc acccagatgt cgcccagcac ctcgtagagc atgcgcgccg
     6601 agcggccggt gacgcgctcg ccgcgcagtt cgtcgaggat cgcccagttg tctgcaccga
     6661 gcagacggat gacgatttcg cggtcggaaa acgaggtgta gttgtacgga atttcgcgca
     6721 ggcgagcggt catgatcaaa ccggtatctg tcaaaaacag gattttagcg tattgcggcg
     6781 caacacgcgg ggcaggtaga ccggagaagg gcgtccgggt tcattcagtt tgttgccgcc
     6841 tgcaccacca gccagtagcg cgcgaacttg ccgagcgcca tgaacagtgc gcagggaagc
     6901 cagtgcaggc gcaaccagcc ggcggcaatg cacagggcgt cgccgatcag cggcaaccag
     6961 gagaagagca gggcggcggc gccccaacgg cgcagtcgat cgacctgcgg cagccgctcg
     7021 acgcgttgcc agcgcggcag gaaacgaccg caggcccagg atatcaggcc gcccagcgta
     7081 ttgccgaggg tcgccacgag cagggccggg taggcctgag tcggcgcgta cttcaggaaa
     7141 gcatagaggg ccagttccga accgccgggc agcagcgtgg ccgccagcag gctgtagagg
     7201 cccagccccc acagcccggc cccgggatcg aaacccgtca acatcaggta cggaaacggg
     7261 cggcgccttc ttccaggctg cgcgcgacat tctccagggc gcgcacctca ccgagcaggg
     7321 cgctcgcctg ctggctgttc gaatcggcca gattggcgac gcgttcgacc gcctgggcga
     7381 tggcctggct ggtcacgccc tgttcgcggg cggcgttgct gatttccgaa gcggcc
//
LOCUS       DAOSDX010000019         7288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3739_length_7288_cov_3.875017, whole genome shotgun sequence.
ACCESSION   DAOSDX010000019 DAOSDX010000000
VERSION     DAOSDX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3739_length_7288_cov_3.875017"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..648)
                     /locus_tag="PLN02_00825"
     CDS             complement(184..648)
                     /locus_tag="PLN02_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50894.1"
                     /translation="MNSTVFLTVLSGVITFVIGQLVLKLVIDPVQELKKTIAQISHSM
                     IERANVIANPGVPSDDVMSETSKHFRQLSSQLHAHLYLIPLFNVTAKIFRLPTKEKIL
                     AASSSLIGLSNSIFRASATEHIHNHNARRVENVCDSLGIYITEGDRVPKDQA"
     gene            complement(736..1305)
                     /locus_tag="PLN02_00830"
     CDS             complement(736..1305)
                     /locus_tag="PLN02_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50895.1"
                     /translation="MNATDIATIAATVLAVVPLVAGTYKFAHAKGVKEGRRELNYARE
                     LEALQHLYAPLVSLFLDVHISSCTLTRYPTFRQRLKHAIAIFRERRYLKFKLKHAFQA
                     LFDKGTSEPSVGVDYGPGFPLSKIGGILKHQTHLAPGELISLYRTMSRDEYERIGASE
                     DELFACHLEFLDHIFDEHRRLSERVRSNP"
     gene            1702..6789
                     /locus_tag="PLN02_00835"
     CDS             1702..6789
                     /locus_tag="PLN02_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HOI50896.1"
                     /translation="MKTGFSISMRGLLVVALALLALALGAYLQFGPGAVSGDDAADEG
                     GAFEVVDAAHREFDGSPALALSFSLPLDARGNPGKFVEVLEMPPRQGEPVAGETDEDS
                     AESSEAMETKVSSSEADTVLDGGKPVAGAWVVGENPRLLFFPNVKPQTRYIIRVQPGL
                     PARNGSTLGGEVRYSIRTAAVAPAFYFASRGMVLPAGQNGGLPVSTVNVPEVDIQFLK
                     VKPEALPTFLDKVIAGAPLPRSESADDEDEETYYDYENRTTKLQGAIDTWTLDNLNKL
                     TTSAFVGRFVTEQKANRRSVTFIPVEDIPALREPGVYIAVMSQPNRFRQEYQTTYFYV
                     SDLGLHVRRHVAGSADAFVSSLVDGKAVGGVEVSWLSTDGKVLARATTDGDGRANFAE
                     QPAEARVIVARKGEQLSLIALKEPALDLSEFEVGGLTSAPVRLFAYSGRNLYRPGESF
                     DVSVLARDADGRPVPAQPVQAILRRPDGKAQWTANWTPDAKFPGYYRRRIELPADAAT
                     GNWYLELRGDPASALASTTLDFNVEEFLPERMKLDLGADKTPLKPGDTWQLAVTGNYL
                     YGAPAAGNRLLGVVNTARNASPLAATLPGFVFGDADEDSTRSRQELAESQLDDAGKAA
                     VDIDFSSLEGRSSPFTVRATLSLLESGGRPVIRSIERTWWPAPELVGVRPLFAGAYAR
                     EGSRAEFEVVLADNAGKLKAGKALPVRMFRENRDWYWRFDDQRGWHSGYTETDELVAS
                     TQVSMPAGGRGKLSLPVKYGRYRIEIVNPATQEVTRYRFYAGWSAQDDESQGVRPDRV
                     GLKLDKAAYKEGETARLTITPPHAGEALITVEGDKLLWSKRISLGSDATTIDLPIAAE
                     WKRHDLYVTALVLRPGNAGASVTPARALGIAHLPLARDERKLAVSLEAPKKMEPDRPL
                     KVKVKVPGAKGQNALVTLSAVDVGILNITNFKSPDPFAFFFAKLRYGQDLHDVYGRLI
                     EKMGGRKGKLKWGGDAAPKATRSLPKKVRLVDLFSGPVALDANGEAEISLPVPDFNGS
                     LRLMAVASAGERFGMQEAEVTVAAPLVVELATPRFLSVGDSALLALDVHNLAGSEQEV
                     KIHVSNDAGLVIRNGEQKFSLKDQQKRILRIPLEAGTAHGLTEVRVHIESPRSKLERT
                     FPLQVQASTPRQTVIRRYIVNPGETLEVRDAELAGFYPQTVGATLAVSDKAPIDVRSA
                     VKGLLTYPYGCAEQTTSTAYPHVFIDEAAARQFGLKPYTQARRAEMLDKAIARLAGMQ
                     APNGGFSLWGNYSEYQYWLSAYVSNFLVDAREQGFAVPPEMEKKALDFLLKGLQEGVA
                     GLPRGPLAYNENSVWNDWRYAGSGRFAVLAYGAYVLATQGKAPLATLRQLAEASERSH
                     SGLALIHLGLALKLMGDEPRSKTAIDAGLKKPRDNRYWWGDYGSNLRDWALTYVLLQK
                     HGLAPEGRDNLVTLVAADMEKQHYLSTQEKLAVFLLGRSFASQPGGEWQAEFVQGSRP
                     PAIGGKGTQFRPLDGGELAAGVKLKNTHGERLFVELNFSGNPAKAPAAKREVFDLERE
                     WYTADGKPLAKRPLRVGESLIVRLKVKTRGRYANALVVDYVPAGFEIENANIVQGEQN
                     ETAIAGIYASQAMQDARIKHVEFRDDRFVVAARLEGDMNFFYRVRVVTPGRFVVPPTY
                     AEDMYQPMIYGLSNGGEPITISDGQAQ"
     gene            6814..>7288
                     /locus_tag="PLN02_00840"
     CDS             6814..>7288
                     /locus_tag="PLN02_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOI50897.1"
                     /translation="MKLSRRGLRWALAALLGALLIADRLAPPPLPGRDTARAQVVLAR
                     DGTPLRAFPDRSHVWRHPLRLDEVSPRYIDAVLAYEDRFFYWHFGVNPVALLRAAGQW
                     LWHGRIVSGGSTLTMQVARLLEPVPRTPAGKLRQMLRAVQLELRLSKREILELYLS"
BASE COUNT         1326 a         2364 c         2373 g         1225 t
ORIGIN      
        1 tgtatccaaa agacaggatg taaacacaat tccgcaaatg tgaacagccc ctagcgacag
       61 aagctgcaat aagaagtgcg acgctgagga gaagtgtcat ggcgtagcgg cccgacgttt
      121 gagttcagcg gacgggcaaa aagcgtagct tttgcgccgt ccgctggaac gatatgttcg
      181 gcgtcatgct tgatccttgg gaacacgatc accctccgtt atatatatac ctaatgagtc
      241 acagacgttc tccacgcgtc ttgcgttgtg attatggata tgctcggtgg cacttgcacg
      301 gaaaatgcta tttgaaagtc cgatcaacga gcttgaggca gccaatatct tctccttagt
      361 gggaagtcga aagatctttg cagtcacgtt gaacaacggg attagataga ggtgtgcatg
      421 caattgtgac gatagctggc gaaaatgctt tgatgtctcg ctcattacgt catcactggg
      481 cacgcccgga ttggcgatta cattcgcacg ctcaatcatg gagtgggaga tttgcgcgat
      541 tgtcttcttc aattcttgaa ctgggtctat caccaacttc aaaaccaatt gaccaattac
      601 aaaggtaatg acgccggaca gcacagttaa aaatacggtt gagttcatca aagtcctcgt
      661 gacgccgaac gtggaggtca ggggacgctg cgcggcgtta tcgcgcagcg tcccctgcac
      721 cgcagggagg gggcattagg gattactcct gacacgttca ctaagccttc tgtgctcatc
      781 gaagatgtgg tccagaaatt ctaggtggca cgcgaatagc tcatcctcgg atgcgccaat
      841 acgctcgtat tcgtctcggg acattgttcg gtagaggctg atgagttcgc caggagcaag
      901 gtgcgtttgg tgtttcaaga ttccgccaat ctttgacagg ggaaatccgg gtccgtagtc
      961 gacgccgacc gatggctcgg aggtgccttt gtcgaagagt gcttgaaagg cgtgtttcag
     1021 cttgaacttc agatagcgcc tttctcgaaa aatagcgata gcgtgcttga gacgttggcg
     1081 gaacgttggg tatcgcgtca gcgtacagga cgagatatga acgtcgagga atagcgatac
     1141 gagtggggcg tacaagtgtt ggagggcttc caactcgcgc gcatagttaa gctctcgccg
     1201 gccctccttc actcccttcg cgtgtgcaaa cttatacgtt ccggcgacaa gagggacaac
     1261 tgcaagaacc gtcgcggcaa tcgtggcaat gtcggtagcg ttcatatgcc ccctaacagt
     1321 tattcacgag cggaaatgtc cgggcgatgg ctaataatgc ggacacgatt ggatcgcctc
     1381 taaggttttg aatggcatag agattatccg atactggtct ccttatcaat caacgatcat
     1441 acggacagag ccagtgatcc gcgatcgagt aaggccttcg gctttcgctc ctgatcgcta
     1501 gtagctgccg gtttgtctga ttgaatctag cagtccgcga taggcgggca aagcgcctct
     1561 ccgaggcatc ggcacactcg tcactgtgtc ggtgaagatt ccgccaggca gcctttcccg
     1621 gccatcccga agtcattttc cggtagagtt cgcctcctat gatgtctgag gggcgatttc
     1681 gctcctgatc ggggggatgg catgaaaacc gggttttcca tttctatgcg cggcctgctg
     1741 gtcgtcgcgc tggccttgct ggcgcttgcg ctgggggcgt atctgcagtt cgggccggga
     1801 gcggtatccg gcgacgatgc ggcggacgaa ggcggcgctt tcgaggtggt cgatgcggcg
     1861 caccgcgagt tcgacggttc gccggcgctg gctttgtcgt tcagcctgcc gctcgatgcc
     1921 cggggcaatc ccggcaagtt cgtcgaagtg ctcgagatgc cgccgcgcca gggtgagccg
     1981 gtggccggcg agaccgacga ggacagcgcc gagtccagcg aagcgatgga aaccaaggtc
     2041 agcagcagcg aggcggatac cgtgctcgat ggcggcaagc cggtggccgg ggcctgggtg
     2101 gtcggcgaga atccgcgcct gctcttcttc cccaacgtca agccgcagac ccgctacatc
     2161 atccgcgtcc agcccggcct gccggcgcgc aatggcagca cgctgggcgg cgaggtgcgt
     2221 tattcgatcc gcaccgcagc ggtggcgccg gccttctatt tcgccagccg cggcatggtg
     2281 ctgccggccg gccagaacgg cggcctgccg gtgagcacgg tgaatgtgcc ggaggtggac
     2341 atccagttcc tcaaggtcaa gccggaagcc ctgcccacct tcctcgacaa ggtgatcgcc
     2401 ggggcgccgc tgccgcgcag cgagtcggcc gacgacgagg acgaggagac gtattacgac
     2461 tacgagaacc ggaccaccaa gctgcagggt gccatcgaca cctggacgct ggacaacctg
     2521 aacaagctga ccaccagtgc cttcgtcggc cgtttcgtca ccgaacagaa ggccaaccgg
     2581 cgcagcgtca ccttcattcc ggtcgaggac atcccggcct tgcgcgaacc tggcgtctac
     2641 atcgcggtga tgtcgcagcc caaccgtttc cgtcaggagt accagaccac ctacttctac
     2701 gtcagcgatc tcggcctcca tgtgcgccgc catgtcgccg gtagcgccga cgccttcgtc
     2761 agctcgctgg tcgacggcaa ggcggtcggc ggcgtcgagg tttcctggct gtccaccgac
     2821 ggcaaggtgc tggcccgcgc caccaccgat ggcgacggcc gcgccaattt cgccgagcag
     2881 ccggccgagg cgcgggtgat cgtcgcccgc aagggcgagc agttgtcgct gatcgcgctc
     2941 aaggaaccgg cgctcgacct ctcggaattc gaggtcggtg gtctgacctc ggcgccggtc
     3001 cggctgttcg cctacagcgg ccgcaatctc taccggccgg gcgagagttt cgacgtctcg
     3061 gtgctggcgc gcgatgccga cggccgcccg gtgccggcgc agccggttca ggccatcctg
     3121 cgccggccgg acggcaaggc gcaatggacg gcgaactgga cgccggacgc caagttcccc
     3181 ggctactacc ggcggcgcat cgaactgccg gccgacgcgg caaccggcaa ctggtatctc
     3241 gaactgcgcg gcgacccggc atcggcgctg gccagcacga cgctcgattt caacgtcgag
     3301 gaattcctgc ccgagcgcat gaagctcgac ctcggtgccg acaagacgcc gctcaagcct
     3361 ggcgacacct ggcaactggc ggtcaccggc aattacctgt acggcgcgcc ggcggccggc
     3421 aaccgcctgc tcggcgtggt caacacggcg cgcaatgcca gcccgctggc cgccaccctg
     3481 cccggcttcg tcttcggcga tgccgacgag gacagcacgc gcagccgcca ggagttggcc
     3541 gaaagccagc tcgacgacgc cggcaaggcg gcggtggaca tcgatttctc cagccttgag
     3601 gggcgtagct cgcccttcac cgtgcgtgcc acgctgagcc tgctggaaag cggcggcagg
     3661 ccggtgatcc gcagcatcga gcggacctgg tggccggcgc cggaactggt cggcgtccgg
     3721 ccgctgtttg ccggtgccta cgcccgcgaa ggctcgcgcg ccgaattcga ggtggtgctg
     3781 gccgacaacg ccggcaagct caaggccggc aaggccttgc cggtccgcat gttccgcgag
     3841 aaccgcgact ggtactggcg tttcgacgac cagcgcggct ggcattccgg ctacaccgaa
     3901 accgatgagc tggtcgcctc gacccaggtc agcatgccgg ccggcggtcg cggcaagctg
     3961 tcgctgccgg tcaagtacgg ccgctaccgg atcgagatcg tcaatccggc cactcaggag
     4021 gtgacgcgtt accgcttcta cgccggctgg tcggcgcagg acgacgaaag ccagggcgtg
     4081 cgcccggacc gcgttggcct caagctcgac aaggcggcct acaaggaagg cgaaaccgcc
     4141 cgcctgacga tcaccccgcc gcacgccggc gaggcgctga tcaccgtcga gggcgacaag
     4201 ctgctgtggt cgaagcggat cagcctgggc agcgacgcaa ccaccatcga cctgccgatc
     4261 gccgccgaat ggaagcgcca cgacctctac gtgacggcgc tcgtcttgcg tcccggcaat
     4321 gccggcgcca gcgtgacgcc ggcccgcgcg ctgggcatcg cccacctgcc gctggcgcgc
     4381 gacgagcgca agctggcggt cagcctggag gcgccgaaga agatggagcc cgaccggccg
     4441 ctcaaggtca aggtcaaggt gccgggcgcc aagggtcaga atgcgttggt gacgctgtcg
     4501 gcggtcgatg tcggcatcct caacatcacc aatttcaaga gccccgatcc tttcgccttc
     4561 ttcttcgcca agctgcgtta cggccaggac ctgcacgacg tctacgggcg cctgatcgag
     4621 aagatgggcg gccgcaaggg caagctcaag tggggcggtg acgcggcgcc gaaggcgacg
     4681 cgcagcctgc cgaagaaggt gcggctggtc gacctgttca gcggcccggt cgcgctcgac
     4741 gccaacggcg aggcggaaat cagcctgccg gtgcccgact tcaacggcag cctgcggctg
     4801 atggcggtgg ccagcgccgg cgagcgcttc ggcatgcagg aggccgaggt cacggtggcg
     4861 gcgccgctcg tcgtcgaact ggcgacgcca cgcttcctct cggtcggcga ttcggccctg
     4921 ctcgccctcg acgtgcacaa cctcgccggc agcgagcagg aagtgaagat ccatgtcagc
     4981 aacgatgccg gcctggtgat ccgcaacggc gagcagaaat tttcgctcaa ggaccagcag
     5041 aagcgcatcc tgcgcattcc gctggaagcc ggcacggcgc acggcctgac cgaggtgcgg
     5101 gtgcacatcg aaagtccgcg cagcaagctc gagcgcacct tcccgctcca ggtccaggcg
     5161 tcgacgccgc gccagacggt gatccgtcgt tacatcgtca atccgggcga gaccctggaa
     5221 gtgcgcgacg ccgaactcgc cggtttctac ccgcagacgg tcggcgccac gctggccgtc
     5281 tccgacaagg cgccgatcga cgtgcgcagc gcggtcaagg gcctgctcac ctacccctac
     5341 ggctgcgccg agcagaccac cagcaccgcc tacccgcacg tcttcatcga cgaggcggcg
     5401 gcccgccagt tcggcctcaa gccttacacc caggcccggc gcgccgagat gctcgacaag
     5461 gcgattgccc gcctggccgg catgcaggcg ccgaacggcg gcttcagcct gtggggcaac
     5521 tattccgagt accagtactg gctctcggcc tacgtcagca acttcctggt cgatgcgcgc
     5581 gaacagggct tcgcggtgcc gccggaaatg gagaagaagg cgctcgactt cctgctcaag
     5641 ggtctgcagg aaggcgtcgc cggcctgccg cgcgggccgc tcgcctacaa cgagaacagc
     5701 gtctggaacg actggcgcta cgccggctcc gggcgcttcg cggtgctcgc ctacggcgcc
     5761 tacgtgcttg ccacgcaggg caaggcgccg ctggcgacgc tgcgccagct ggccgaagcc
     5821 agtgagcgct cgcactccgg tctggccctg atccacctcg gcctggcgct caagctgatg
     5881 ggcgacgagc cgcgcagcaa gacggcaatc gacgccggcc tcaagaagcc gcgcgacaac
     5941 cgctactggt ggggtgacta cggcagcaac ctgcgcgact gggcgctcac ctacgtgctg
     6001 ctgcagaagc acggcctggc gccggaaggc cgcgacaacc tggtgaccct ggtcgctgcc
     6061 gacatggaga agcagcatta cctgagcacc caggaaaaac tggccgtctt cctgctcggg
     6121 cgcagtttcg cgagccagcc gggcggcgaa tggcaggccg agttcgtgca aggcagccgg
     6181 ccgccggcga tcggcggcaa gggcacccag ttccggccgc tcgacggtgg cgaactggcg
     6241 gccggcgtca agctgaagaa cacgcatggc gaacgcctct tcgtcgaact caacttcagc
     6301 ggcaacccgg ccaaggcccc ggccgccaaa cgcgaagtct tcgacctcga acgcgagtgg
     6361 tacaccgccg acggcaagcc gctcgccaag cgcccgctgc gcgtcggcga aagcctgatc
     6421 gtccgcctca aggtgaagac gcgcggccgc tacgccaacg cgctggtcgt cgattacgtg
     6481 ccggccggat tcgagatcga gaacgccaac atcgtccagg gcgagcagaa cgagacggcg
     6541 attgccggca tctacgccag ccaggcgatg caggacgcgc ggatcaagca cgtcgagttc
     6601 cgcgacgacc gcttcgtcgt cgccgcccgc ctcgagggcg acatgaactt cttctaccgc
     6661 gtgcgggtgg tgacgccggg ccgcttcgtc gtcccgccga cctacgccga ggacatgtac
     6721 cagccgatga tctacggcct gagcaacggc ggcgagccga tcacgatcag cgatggccag
     6781 gcgcagtaag ccggcgcgcc gcggcggggg gcgatgaagc tctcccggcg cggtctgcgc
     6841 tgggcgctgg cggccctgct cggcgccttg ctgattgccg accggctggc gccaccgccg
     6901 ctgcccgggc gcgacacggc gcgtgcccaa gtggtgctgg cgcgcgacgg cacgccgttg
     6961 cgcgccttcc ccgaccgcag ccacgtctgg cgtcatccgc tgcgcctcga cgaggtatcg
     7021 ccgcgttaca tcgacgccgt gctcgcctac gaggaccgct tcttctactg gcacttcggc
     7081 gtcaacccgg tggccctgct gcgcgccgcc gggcagtggc tgtggcacgg ccgcatcgtc
     7141 tccggcggct cgacgctgac catgcaggtg gcgcgcctgc ttgaaccggt gccgcgcacg
     7201 ccggccggca agctgcgcca gatgctgcgc gcggtgcagc tcgaactgcg cctgtcgaag
     7261 cgcgagatcc tcgagcttta cctgagct
//
LOCUS       DAOSDX010000020         7101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3862_length_7101_cov_2.925490, whole genome shotgun sequence.
ACCESSION   DAOSDX010000020 DAOSDX010000000
VERSION     DAOSDX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3862_length_7101_cov_2.925490"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PLN02_00845"
     CDS             complement(<1..1365)
                     /locus_tag="PLN02_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOI50898.1"
                     /translation="MTAIAFVDLETTGATAASDRITEIGIVEVDADGSVREWQQLVNP
                     GRRIAPFVERLTGISNAMVASAPPFADVAEEVRRRLQGRLFVAHNASFDYGFLKSEFR
                     QLGIDFRAPVLCTVKLSRALYPEFKRHSLDALIERHGLHASGRHRALADARLIHQFWQ
                     KIHDDRSADEIASALAELNPYSSLPPHIDAEIVDALPDTPGVYLLYGSRNDQDDLPLY
                     VGKAKNIRGRVLALFSGKQRSPRERALVQQTRRIDWIQTAGEIGTRLRETALVKQLRP
                     TQNGPLRDDEEVCTWTFINAGDGWLRPQLSLVRNLGVGTGKACYGLFSNARQAKEFLR
                     NLVETNQLCDVLSGLEHAPAGQACPGHAAGRCKGACVGKETLASHSARLVGALVALKL
                     PSWPFPGPAVIHEGDEIHVIDGWRYLGTVRSTGEIDALLAAGRPPFDHSTYTILNKHL
                     RLMKP"
     gene            complement(1469..1729)
                     /locus_tag="PLN02_00850"
     CDS             complement(1469..1729)
                     /locus_tag="PLN02_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50899.1"
                     /translation="MSDDSGLWLLLAGPVGATALYWTLYRYYRNTDKSHGFERETKVR
                     AQPVAGTDIKVDEITGTQRTRINGDNVSDYRTRVQRIRDEFS"
     gene            complement(1726..3030)
                     /locus_tag="PLN02_00855"
     CDS             complement(1726..3030)
                     /locus_tag="PLN02_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50900.1"
                     /translation="MADSERLSPRPVNGPGSPRDVPPRPGTQPVDPALPEAIRQELEA
                     PDPAIIDTAATELGDGINRCPKCGSTDIRHKPGSDSLVCLYCRNEWDGTRVEELFGLG
                     EGIADLQGTVIASGARDIAADAATLMTFKCSGCGAEVTINTENAMTARCHWCRQTLGV
                     NEQIANGAVPDAVLPFRIRKEDAVARIRQFVDKRRLFALKAFKAQFTPENVIGVYLPY
                     MIVDGNVSVDVAGKGEIKTREYTRGSDKNKQTYYDADIYQVDRHVDFTVDDLPLESSS
                     ERGNLDTRANTNNIINTILPFDTKNAVKWNASYLSGFSSEKRDSNIEHLRPRLEDQLL
                     SIGRAQVENSVRRYNRGVRWEQEKLDVHGSRWVSMYLPVWLYSYHQTGANGGMLHYIA
                     VNARTGETMGSVPVQQWKLLLAALTVGTILETIAIAILVNFS"
     gene            complement(3043..4179)
                     /locus_tag="PLN02_00860"
     CDS             complement(3043..4179)
                     /locus_tag="PLN02_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOI50901.1"
                     /translation="MGLLRAVSGAVGGVLADQWKDFYTVPAGLPSTAALFAAVPQGTN
                     AGRGSNTSGSSNIISNGSKIVVPEGYGLLLFQDGKITAFVAEPGGYEWRSDDLNSQSI
                     FAGDNLVSALIMQSWERFKFGGQPGSQQAAFFVSLKELPDNRFGTQSEIYWDDAFLNT
                     QVGAITRGSYTLKIVDPILFVKNFVPASYLQASRVFDFSDFDNAAASQLFNEVVASLA
                     PAFSLYTNDPSKGNRIARIQQDTVGFAKSLSEAVEQAYQWKSDRGLAITKTAIVSIEY
                     DAATRELLKTVQRADALSGVRGNSNLQASVAQGIQSAGEHSGAAGLVGIGMATGGLAG
                     LQQQPVAPAADDPVAKLKKAKEMLDLGLITQADYDALKAKALGL"
     gene            complement(4399..5805)
                     /locus_tag="PLN02_00865"
     CDS             complement(4399..5805)
                     /locus_tag="PLN02_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HOI50902.1"
                     /translation="MKKTFMGLRLKRLREERQMTQVALAQALGLSPSYLNQLEQNQRP
                     LTVSVLLRLNAAFGIDVQLFSEDDEARLISELRDLFADAPNDDAPGLAEIRELASRLP
                     GVARLLLQQSRQLHEAREREAIWASRLGLDRQAETLPRPMPFEAVRDFFYRRHNHIAE
                     LDELAEGCFADWQLRPGDTGNGLARRLSERHGILVSTDDNDRGLLRDFDEKARRLRLS
                     ASLSSGQQAFHMATQLARLEAGELIDELAAGAAPDDEETRRLARIGLASYFAGALLLP
                     YGRYLESAEACRYDIEQLGRSYGVGYETACHRLSTLQRPQAPGVPFFFIRVDRAGNIS
                     KRQSATDFHFSRIGGTCPLWNVYEAFAQPGKILTQLAAMPDGRNYLWIARSVHRGHGG
                     YGAPGKTFSIGLGCDLRHAERLVYSKGLDLKAVDGFTPIGTACKVCPRAHCPQRAFPP
                     SGNALVVDENRRYFSPYA"
     gene            6063..6731
                     /locus_tag="PLN02_00870"
     CDS             6063..6731
                     /locus_tag="PLN02_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI50903.1"
                     /translation="MCGLSRQTAADLPGFGGQALYEGIADSLRERIFSHRLAPGSPVD
                     EVALARHYGVSRTPVREALKVLASEGLLEVTPYRGCRVARIAGDDLAAALDVVELVAR
                     HALLRLLGQAEAAGRMRLSQAAAAGGSWAGFCHIAVELLAQPSLAAVSRNLHQQLQLC
                     LGPALAFADRGGSPALRAALRQAIEQADPAAVLAAYRELAADFRVSLLGACDEQQREI
                     AVSG"
     gene            6803..>7101
                     /locus_tag="PLN02_00875"
     CDS             6803..>7101
                     /locus_tag="PLN02_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50904.1"
                     /translation="MTDDLFPLPPIIETAPSCDIQGLSADTHGVALAGALLWSYFTRT
                     QVHELLSLLALKNAAGRNFTGEQVKTALQELNQRQLLDEHAQRPGYFRLVDSLRVP"
BASE COUNT         1110 a         2190 c         2460 g         1341 t
ORIGIN      
        1 cggtttcatc agccgcaggt gcttgttcag gatggtgtaa gtgctgtggt cgaaaggcgg
       61 ccggccggca gcaagcagcg catcgatctc gcctgtgctg cgtaccgtgc cgaggtagcg
      121 ccagccgtcg atgacgtgaa tctcgtcgcc ctcgtggatc accgccgggc cggggaaggg
      181 ccagctcggc agcttgaggg cgaccagcgc gccgaccagg cgggcgctgt ggctggccag
      241 cgtttccttg ccgacgcagg cgcccttgca gcgtccggcg gcgtgccccg ggcaggcctg
      301 cccggccggc gcgtgttcaa ggccgctgag gacatcgcag agctggttgg tttcgaccag
      361 gttgcgcagg aattccttgg cctggcgcgc gttgctgaac agcccgtagc aggccttgcc
      421 ggtcccgacg ccgaggttgc ggaccaggct cagttgcggc cgcagccagc cgtcgccggc
      481 gttgatgaag gtccaggtgc agacttcctc gtcgtcgcgc agcgggccgt tctgggtcgg
      541 ccgcaactgc ttgaccagcg ccgtttcgcg tagccgggtg ccgatctcgc cggcggtctg
      601 gatccagtcg atgcgccgcg tctgctgtac cagcgcgcgt tcgcgcggcg aacgctgctt
      661 gccggaaaag agggccagga cccggccgcg gatgttcttt gccttgccga cgtagagcgg
      721 caggtcgtcc tggtcgttcc ggctgccgta gagcagatag acgccggggg tgtcgggcag
      781 cgcgtcgacg atttcggcgt cgatgtgcgg cggcaggctg gaataggggt tcagttcggc
      841 gagcgccgag gcgatttcgt cggcgctgcg gtcgtcgtgg atcttctgcc agaactggtg
      901 gatcaggcgg gcgtcggcca gcgcccggtg gcggccgctg gcgtgcaggc cgtgccgctc
      961 gatcagcgca tccaggctgt ggcgcttgaa ttccgggtag agcgcgcgcg acagcttgac
     1021 ggtacacaac accggcgccc ggaagtcgat gccgagctgc cggaattcgc ttttcaggaa
     1081 gccgtagtcg aagctcgcgt tatgggcgac gaacagccgc ccctgcagcc ggcgccggac
     1141 ttcttcggca acatcggcaa agggtggggc gctggcgacc atggcattgc tgatgccggt
     1201 caggcgttcg acgaaagggg cgatgcgccg tcccggattg accagttgtt gccactcgcg
     1261 cacgctgcca tcggcatcga cttcgacgat accgatttcc gtgatgcggt cgctggcggc
     1321 agtggcgccg gtcgtttcca ggtcgacgaa ggcgatggcg gtcatggccg ggattgtctc
     1381 atgactgggt ggcgtggcga ttcaggggca gggcgaaaaa gtcgttggct gtcaccggca
     1441 ggccgcaatg cccatgactg ttccgtgttc aggaaaactc atccctgatc cgctggaccc
     1501 gggtgcgata gtcgctcacg ttgtcgccat tgatacgcgt ccgctgcgta cccgtgattt
     1561 cgtcgacctt gatgtccgtc cccgccaccg gctgcgccct gaccttggtt tcgcgctcga
     1621 agccgtgcga cttgtcggtg ttgcggtagt aacggtacaa agtccagtac agcgcggttg
     1681 cgccgaccgg gccagccagg agcagccaga gtccgctgtc gtcgctcatg agaaattcac
     1741 caggatcgca atggcgatgg tttcgaggat ggtgccgacg gtgagcgcgg ccagcagtag
     1801 tttccattgc tgcaccggca cgctgcccat ggtttcgccg gtgcgggcat tgaccgcgat
     1861 gtagtgcagc atgccgccat tggcgccggt ctgatggtag gaatacaacc agaccggcag
     1921 gtacatggaa acccagcggc tgccgtgcac atcgagcttt tcctgttccc agcgcacgcc
     1981 gcggttgtag cggcgcaccg agttttcgac ctgggcgcgg ccgatcgaca gcagttggtc
     2041 ttccagccgc ggtcgcaggt gttcgatgtt gctgtcgcgt ttttccgagg agaaacccga
     2101 caggtaggac gcgttccact tcaccgcgtt cttggtgtcg aagggcagga tggtgttgat
     2161 gatgttgttg gtattggcgc gggtgtcgag gttgccgcgt tcgctggacg attccagtgg
     2221 cagatcgtcg acggtgaaat cgacgtgccg gtcgacctgg tagatatcgg cgtcgtagta
     2281 ggtctgcttg ttcttgtcgg agccgcgcgt atattcgcgc gtcttgatct cgcccttgcc
     2341 ggcgacatcg acgctgacgt tgccgtcaac gatcatgtag gggagataga cgccgatcac
     2401 gttctcgggc gtgaactgcg ccttgaacgc cttcagggcg aacagtcggc gtttatcgac
     2461 gaactggcgg atacgcgcca ccgcgtcttc cttcctgatg cggaagggca ggaccgcgtc
     2521 ggggaccgca ccgttggcga tctgctcgtt tacccccagg gtctggcgac accagtggca
     2581 gcgcgcggtc atcgcgtttt ccgtgttgat cgtcacctcg gccccgcagc cggagcactt
     2641 gaaggtcatc agggtagcgg catcggcggc gatgtcgcgg gcgccggagg cgatgacggt
     2701 gccctggaga tcggcaatgc cttcccccag cccgaacagt tcctcgaccc gcgtgccgtc
     2761 ccactcattg cggcagtaca ggcagaccag cgaatcgctg ccgggcttgt ggcggatgtc
     2821 ggtcgaaccg cacttcggac agcggttgat gccatcgccg agttcggttg cggcggtgtc
     2881 gatgatggcc gggtcgggcg cctccagctc ctggcggatc gcctcgggca gggccggatc
     2941 gactggctgg gtacccgggc gcggcggcac gtcgcgcggc gaaccggggc cgttgacggg
     3001 ccgcggcgac aggcgttcgg aatcggccat ggtcgcgctg cgctacaggc ccagcgcctt
     3061 ggccttgagc gcgtcgtagt cggcctgggt gatcaggccg agatcgagca tctccttggc
     3121 tttcttcagc ttggcgacgg gatcgtccgc cgccggggcc accggttgct gctgcagccc
     3181 ggcgaggccg ccggtggcca tgccgatgcc gaccagcccg gcggcgccac tgtgttcgcc
     3241 ggccgactgg atgccctgcg cgacgctcgc ctgcaggttc gagttgccgc gcacaccgga
     3301 cagcgcatcg gcgcgctgga ccgtcttcag caactcacgt gtagccgcgt cgtattcgat
     3361 cgagacgatg gcggtcttgg tgatcgccag gccgcgatcg gatttccact ggtaagcctg
     3421 ctcgaccgcc tcggacaggc ttttggcgaa gccgaccgta tcctgctgga tgcgggcgat
     3481 acggttgccc ttgctcggat cgttggtgta cagactgaag gcgggcgcca gcgaagcgac
     3541 cacctcgttg aacaactggc tggcggcggc gttgtcgaag tcgctgaaat cgaaaaccct
     3601 gctcgcctgc aggtagctgg ccggcacgaa gttcttcacg aacaggatcg ggtcgacgat
     3661 cttcagcgtg tacgaaccgc gggtgatcgc gccgacctgc gtattgagga aagcatcgtc
     3721 ccagtagatc tcggactgcg tgccgaagcg gttgtccggc aattccttca gcgaaacgaa
     3781 gaatgcggcc tgctgcgaac cgggctggcc accgaacttg aaacgttccc agctttgcat
     3841 gatcagcgcg ctgacgaggt tgtcgccggc gaagatcgac tgcgaattga gatcgtccga
     3901 gcgccactca tagccgcccg gctcggcgac aaaggcggtg atcttgccgt cctggaaaag
     3961 cagcagcccg tagccctcgg ggacgacgat cttcgagccg ttgctgatga tgttggagga
     4021 gccgctggtg ttcgagccgc gcccggcatt ggtgccctgc ggcactgccg cgaacagtgc
     4081 cgccgtggac ggcaagccgg ccggcaccgt gtagaagtct ttccactggt ccgccaatac
     4141 accgccgacc gcaccgctca ccgcccgcaa aagacccatg acaactcccc gctgaagatt
     4201 cgttgagacc accagagacc agccgcccgg caaggcgcga tgccgattcc gaccgggaaa
     4261 cgcgccaggg gcgatgctcg atgacttgat cggtggcaag tatccccggg cctttcaccg
     4321 catgtcaaac gggacagcaa aggcgcgggc tgatcatggt catggctggc gtcgtcccat
     4381 ggcggggcgt agggcgcttc aggcgtaggg cgagaagtac cgtcggttct cgtcgacgac
     4441 cagggcgttg ccgctgggcg ggaaggcgcg ctgcgggcaa tgggcgcgcg ggcagacctt
     4501 gcaggccgtg ccgatcggcg tgaagccgtc tacggccttg aggtcgaggc ccttgctgta
     4561 aaccaggcgc tcggcgtggc gcaggtcgca gccgaggccg atcgagaagg tcttgcccgg
     4621 ggcgccatag ccgccgtggc cgcggtggac gctgcgggcg atccacaggt agttgcggcc
     4681 gtcgggcatg gcggcaagct gggtgaggat cttgccgggc tgggcgaagg cttcatagac
     4741 gttccacagc ggacaggtgc cgccgatgcg cgaaaagtgg aagtcggtcg ccgactggcg
     4801 cttcgaaatg ttgccggcgc gatcgacgcg gatgaagaag aaggggacgc ccggggcctg
     4861 cgggcgttgc agcgtggaca gtcgatggca ggcggtttcg taaccgacgc cgtagctgcg
     4921 gccgagttgt tcgatgtcgt agcggcaggc ttcggcgctt tccagatagc ggccgtaggg
     4981 cagcagcaag gcgccggcga agtagctggc cagtccgatg cgcgccaggc gccgggtttc
     5041 ctcgtcgtcc ggggcggcgc cggcggccag ttcgtcgatc agttcgccgg cttcgagccg
     5101 ggccagttgt gttgccatgt ggaaggcctg ctggccgctg ctgagcgacg ccgacaggcg
     5161 cagacggcgg gctttctcgt cgaaatcacg cagcagcccg cgatcattgt cgtcggtgct
     5221 gaccaggatg ccgtgccgct cgctcaggcg gcgggcgagg ccgttgccgg tgtcgccggg
     5281 gcgcagttgc cagtcggcga agcagccctc ggcgagttcg tcgagttcgg cgatatggtt
     5341 gtggcggcgg tagaaaaaat cgcgcaccgc ttcgaaaggc atcggtcgcg gcagtgtctc
     5401 ggcctgccgg tcaaggccga gacggctcgc ccagatcgcc tcgcgttcgc gtgcctcgtg
     5461 cagttgccgg ctctgttgca gcagcaggcg ggcgacgccg ggcaagcggc tggccagttc
     5521 gcggatttcc gccagccccg gtgcatcgtc gttcggcgca tcggcgaaga ggtcgcgcag
     5581 ttcgctgatc aggcgcgctt cgtcgtcctc ggaaaatagc tggacatcga tgccgaaagc
     5641 cgcgttcagg cgtaacaaca ccgacaccgt cagcggccgc tggttctgtt cgagctggtt
     5701 caggtagctc ggcgagaggc cgagcgcctg cgccagcgcg acctgcgtca tctgccgttc
     5761 ctcgcgcagg cgcttcaggc gcaggcccat gaaggttttt ttcacaagga ttcctcagcg
     5821 aattcgcaaa attcgcaaac gcgtgctgct tgtttcgcaa agtcacgcaa tttcagtggt
     5881 ttatgaattt tttgcgatgc gtatattgca aaaaaccggc gccgccggca agtcaatctt
     5941 catcatcctt tcgggagatc atgcatgttc gtcaatccct acctctattt cgaagaactc
     6001 ttcccttcgc cgccgccaag cccgccggat gaggcggtgg tgcaagcgga aagcgacgcg
     6061 ccatgtgcgg actgagtcgc cagacggctg ccgatttgcc cggtttcggc ggtcaggcct
     6121 tgtacgaagg catcgccgat tcgttgcgcg aacgcatctt ctcgcaccgg ctggcacccg
     6181 gtagcccggt cgacgaggtg gcgctggccc gccattacgg cgtcagccgg acgccggtac
     6241 gcgaggcgct gaaggtattg gccagcgagg ggctgctcga ggtcacgcct tatcgtggtt
     6301 gccgggtggc tcgcatcgcc ggcgacgatc ttgccgcggc gctcgacgtc gtcgaactcg
     6361 tcgcgcgtca cgcgctgctt cgcctgctcg ggcaagccga ggcagcgggg cgcatgcggc
     6421 tgagtcaggc tgctgcggcg ggcggtagct gggcggggtt ttgccacatc gccgttgaac
     6481 tgctcgccca gccgtcgttg gcggcggtca gccgcaacct gcaccagcaa ctgcagctct
     6541 gcctcggccc ggcgctggca ttcgccgacc ggggcgggtc gccggcgctg cgcgcagccc
     6601 tgcgccaggc tatcgaacag gccgacccgg cggcggtgct ggcggcgtat cgtgagttgg
     6661 cggctgattt tcgcgtcagt ctgctcggcg cctgcgatga acaacagcgg gagatcgcgg
     6721 tttctggttg acggggggcg cttagggcga aaatacttgc ggtttggcgt cagctttttc
     6781 gccccttcct cctgtccctc ccatgaccga cgatcttttc ccgctgccgc cgataattga
     6841 aaccgccccg tcatgcgaca tccagggctt gtccgccgat acccacgggg tcgctctggc
     6901 cggcgcgctg ttgtggtcgt atttcacccg gacccaggtc catgagctgc tctcgctgct
     6961 ggcactcaag aacgcggccg gacgcaactt caccggcgag caggtcaaga cggcgcttca
     7021 ggaactgaac cagcgccagt tgcttgacga acacgcgcaa cgtcccggtt atttccggct
     7081 ggtcgattcg ctgcgtgttc c
//
LOCUS       DAOSDX010000021         7080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3883_length_7080_cov_3.821637, whole genome shotgun sequence.
ACCESSION   DAOSDX010000021 DAOSDX010000000
VERSION     DAOSDX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3883_length_7080_cov_3.821637"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLN02_00880"
     CDS             complement(<1..858)
                     /locus_tag="PLN02_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadD"
                     /protein_id="HOI50905.1"
                     /translation="MSPKQLVVALTLAIGLPAWAAPPPSEDLLGRTVFQVLLGELALR
                     QGAVGLSVQAWADLAERTRDPKVIARAVEVAGFAGDNARALALVELWLSVEPESSAAR
                     QAQLALLVHARQVDQLQPHLVALLASDKENLNENLLHLNRMLARIPDKQAVLRLLDAV
                     LPPYLELPEARFALAQAALAAGDEARALREAEAALARKPDWDLAAIARAKLVARQDAE
                     KGLGGLRRFVDAHPTARDARLALAQMLVAEKRLDDARLQYERLLKDFPEEPAVLYPAA
                     ILTLQHGDRE"
     gene            946..1779
                     /gene="mutM"
                     /locus_tag="PLN02_00885"
     CDS             946..1779
                     /gene="mutM"
                     /locus_tag="PLN02_00885"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOI50906.1"
                     /translation="MPELPEVEVCRRGIAPELEGEVVRAVVQRAAKLRQPIPGELREL
                     LPGCRIVAVRRRGKYLLIDCRRADVEGTLIIHLGMSGNLRFVPADLPPEKHDHFDLVL
                     PHQTLRLADPRRFGVVLWQPGPPATVERHPLLAVLGIEPLADDFTGDWLHAALARRHG
                     PVKPVLMDSHMVVGIGNIYAAESLFRAGISPLRAASRISRLRCASLVDAIRATLSDAI
                     AAGGSSIRDYVHSDGGAGCFQIQAGVYDRAGEPCLRCGGAVRQIRQGGRSTYYCPACQ
                     H"
     gene            1846..3795
                     /locus_tag="PLN02_00890"
     CDS             1846..3795
                     /locus_tag="PLN02_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOI50907.1"
                     /translation="MSLANQFAAYSAWRTLLSNDIAEFQHWLADNELSDGQIDLRLSQ
                     LQERLREDRLNVAFVAEFSRGKSELINAIFFAEYGNRMLPSSAGRTTMCPTELLFDAS
                     KQPCIELLPIQTRASNASVSEYKRLADEWTQLPLDGDSPEDMQAALRHVSETIRVTPA
                     EAGELGFEVGKGEADPYRIGADGLVEVPRWRHALINFPHPLLKQGLVILDTPGLNAIG
                     AEPELTLSQLPNAHAVLFILAADTGVTQSDLTIWKEHIASAGNARRGRMVVLNKIDGQ
                     WDELKTAAAIDAEIARQVDSCAAILNLPASQIFPVSAQKGLVAKINDDAALLARSRLP
                     ALEEALSGELIPAKREIVCESTDAEFGDVVARVRALLDSRFAGLREQLAELAELRGKN
                     RGVVEYMMGKIRAEKEEFEAGLQRYYAVRSVFSTLTNNLFGHLGIDRLRQLTGETREN
                     MLEARFSKTLSDAMGGFFARTREALAKSNGEIAEILAMMDAVYKKFAVEHGLKLGAPT
                     AFSLLRYEREIDRLQAWCDTHLNTVVTLLTHDKRNITQKFFEEVAIQVRRAFERANRD
                     AEVWLRAIMAPMETQVREHQIQLKRRLESIKRIHQATDTLEDRIAELDSVGKTLEEQL
                     AMLADIEYRVRDLMTPEQMAELRAA"
     gene            complement(3809..4897)
                     /locus_tag="PLN02_00895"
     CDS             complement(3809..4897)
                     /locus_tag="PLN02_00895"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOI50908.1"
                     /translation="MNKIKVMIVDDSALVRQVITQAISREPSIEVIATAQDPVFAIDK
                     LKAQWPDVFIVDIEMPRMDGLTFLKKIMAERPTPVIICSSLADKGAQVTMEALAAGAI
                     NIITKPKLNLKSFLEDSSNDIISAIKSAARANMRVVRSMTGAARSPLAVAPKQSADAM
                     LASTPHQGARMYGTTDRVVAIGTSTGGTQALEAVLSRLPAVTTGIVIVQHMPEKFTAM
                     FAERLNGLCAMEIREARDGDRVHPGLALIAPGGRHMMLRRNGAQYLVEVRDGPLVNRH
                     KPSVDVLFRSVAQVAGRNALGIIMTGMGDDGARGLKEMRDAGATTIGEDESTCVVYGM
                     PKEAFKLGGVGRELPLDQIPREIVGFGS"
     gene            complement(4894..5397)
                     /locus_tag="PLN02_00900"
     CDS             complement(4894..5397)
                     /locus_tag="PLN02_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOI50909.1"
                     /translation="MSSCLARPEVFLHPGEFHFASAPLRIGTLLGSCVAVTVWHPQHR
                     FGGMCHILLPGRARPPASLPDCRYADEAIERFVYELRSRRVAPANCQIKLLGGGNMFA
                     GSSAGNMEVGQRNVEATRQALAAQGFRVTREHVGGARRRRLTFDLASGHVWLALPGAQ
                     DFSKEQQ"
     gene            complement(5407..6225)
                     /locus_tag="PLN02_00905"
                     /pseudo
     CDS             complement(5407..6225)
                     /locus_tag="PLN02_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385581.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
     gene            complement(6305..6832)
                     /locus_tag="PLN02_00910"
     CDS             complement(6305..6832)
                     /locus_tag="PLN02_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI50910.1"
                     /translation="MSGQPPVVAGDTEQQQYLTFLLGEEMFAIGILAIREIIEYGQIT
                     EVPLVPPFIRGVINLRGAVVPVIDLSVRFGRSAGEITKRTCIVIIEIAETAGAHAQQM
                     GVVVDAVSEVLEIAPGDIEPPPTFGTRIRTDFISGMGKVGGRFVVLLDVERILSLDEV
                     AVIAAINQGELGAVA"
     gene            complement(6881..>7080)
                     /locus_tag="PLN02_00915"
     CDS             complement(6881..>7080)
                     /locus_tag="PLN02_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50911.1"
                     /translation="EEMSSQAGNLQELMAFFNLGNRAAGGTANRRGGSTPATRSAGRR
                     QAEAAPANFAGSLDDSSFARF"
BASE COUNT         1249 a         2405 c         2284 g         1142 t
ORIGIN      
        1 ttcgcggtcg ccgtgctgca gggtcaggat ggcggcgggg taaagcaccg ccggctcctc
       61 cgggaaatcc ttgagcagcc gttcgtattg cagccgggcg tcgtccaggc gcttttcggc
      121 gaccagcatc tgggccagcg ccagacgggc gtcgcgcgcc gtcggatggg catcgacgaa
      181 gcgccggagt cccccgagtc ccttctcggc atcctgacgc gccaccagct tggcgcgggc
      241 gatcgccgcc aggtcccagt ccggcttgcg cgccagcgcc gcttccgcct cgcgcagcgc
      301 ccgcgcttcg tcgccggcgg cgagcgccgc ctgggccagc gcgaaacgcg cttccggcaa
      361 ttccagataa ggtggcagca cggcgtcgag caggcggagc acggcctgct tgtccgggat
      421 gcgcgccagc atgcggttca ggtgcagcag gttctcgttg aggttttcct tgtcgctcgc
      481 cagcagggcg accagatgcg gctgcaactg atcgacctgg cgggcatgga cgagcagtgc
      541 cagttgcgcc tggcgggcgg cactcgattc cggctcaacg ctcagccaca actcgaccag
      601 cgccagggcg cgggcgttgt cgccagcgaa accggcgacc tcgacggcgc gcgcgatgac
      661 cttcgggtca cgggtgcgct cggcgaggtc ggcccaggcc tggacgctga gtccgaccgc
      721 cccctggcgc agcgccagtt cgccgagcag gacctggaac acggtgcgcc cgagcagatc
      781 ttcgctaggc ggcggagcgg cccatgccgg caggccgatg gccagggtga gggcgacgac
      841 gagttgcttc ggggacatgg cggctttccg attggcgtgc gtatttgaac gcataataac
      901 gaagtagttt gcgatgttat ttgggaatcg acgggggaac aagcgatgcc ggagttgccg
      961 gaggttgaag tctgtcgccg ggggatcgcc cccgagctgg aaggcgaggt ggtacgcgcc
     1021 gtcgtgcagc gggcggcgaa gctgcgccag ccgatccccg gcgaattgcg cgagctgctg
     1081 ccgggctgcc ggatcgtcgc cgtgcgccga cgcggcaagt acctgctgat cgactgccgg
     1141 cgggccgacg tcgagggcac gctgatcatc catctcggca tgtcgggcaa cctgcgcttc
     1201 gtccccgccg acctgccgcc ggaaaaacac gaccacttcg acctggtcct gccccaccag
     1261 acgctgcgcc tcgccgatcc ccggcgcttc ggcgtcgtcc tctggcagcc gggaccgccg
     1321 gcaacggtcg agcgtcaccc gctgctcgcc gtactcggca tcgagccgct cgccgacgac
     1381 ttcaccggcg actggctgca cgcggccctg gcgcggcgcc acggcccggt caagccggta
     1441 ctgatggaca gtcacatggt ggtcggcatc ggcaacatct acgccgcgga aagcctgttc
     1501 cgcgccggca tctcgccgct ccgtgcggcc agccggatca gccgcctgcg ctgcgccagc
     1561 ctggttgacg ccatccgtgc caccctgagc gacgccatcg ccgccggcgg cagcagcatc
     1621 cgcgactacg tgcacagcga cggcggcgcc ggctgcttcc agatccaggc cggcgtctat
     1681 gaccgcgccg gcgaaccctg cctgcgctgc ggcggcgccg tccggcaaat ccgccagggc
     1741 ggccgcagca cctattactg cccggcctgt cagcactgaa agcgcgcagc agcgctggcc
     1801 acttatgcta aattggatac tcgtactgtc gccggaacct ccgtcatgtc gcttgccaat
     1861 caatttgccg cctattccgc ctggcggacg ctgctctcca acgatatcgc cgagttccag
     1921 cactggctgg cggacaatga actctccgac gggcagatcg acctccgcct ctcgcaactg
     1981 caggaaagac tgcgcgaaga ccggctcaac gtcgccttcg tcgccgagtt ctcgcgcggc
     2041 aagtcggaac tgatcaacgc catcttcttt gccgagtacg gcaaccggat gctgccctcg
     2101 tcggcgggac ggacgacgat gtgtccgaca gaactgctgt tcgacgccag caagcagccc
     2161 tgcatcgagc tgctgccgat ccagacccgc gcttccaacg ccagcgtcag cgagtacaag
     2221 cgcctggccg acgaatggac gcaattgccg ctcgatggcg actcgccgga agacatgcag
     2281 gccgccctgc gtcacgtcag cgagacgatc cgcgtcaccc cggcggaggc cggcgaactc
     2341 ggcttcgaag tcggcaaggg cgaagccgat ccctaccgga tcggcgccga cgggctggtc
     2401 gaggtgccgc gctggcgcca cgcgctgatc aacttcccgc acccgctact caagcagggc
     2461 ctggtcatcc tcgacacgcc ggggctcaac gcgatcggcg ccgagcccga actgaccctg
     2521 tcgcaactgc cgaacgccca cgccgtgttg ttcattttgg ccgccgatac cggcgtcacc
     2581 cagtccgacc tgaccatctg gaaggaacac atagccagcg ccggcaatgc ccggcgcggc
     2641 cgcatggtgg tactgaacaa gatcgacgga caatgggacg aactgaagac ggcggcggcg
     2701 atcgacgccg aaatcgcccg ccaggtcgat agctgcgccg ccatcctcaa cctgccggcc
     2761 agccagatct tcccggtctc cgcccagaag ggcctggtcg ccaagatcaa cgacgacgcc
     2821 gcgctgctcg cccgcagccg cctgccggcg ctcgaggaag cgctgtccgg cgaactgatc
     2881 ccggccaagc gcgaaatcgt ctgcgaaagc accgacgccg aattcggcga tgtcgtcgcc
     2941 cgcgtccgcg ccctgctcga ctcccgtttc gccggcctgc gcgagcagct cgccgaactg
     3001 gccgaactgc gcggcaagaa ccgcggcgtc gtcgagtaca tgatgggcaa gatccgcgcc
     3061 gagaaggagg aattcgaagc cggcctgcaa cgctactacg ccgtgcgcag cgtcttctcg
     3121 acgctgacca acaacctctt cggccacctc ggcatcgacc gcctgcgcca gctcaccggc
     3181 gaaacccgcg agaacatgct cgaagcccgc ttctcgaaga ccctgtcgga cgccatgggc
     3241 ggcttcttcg cccgtacccg cgaggcgctc gccaaatcga acggcgagat cgccgagatt
     3301 ctcgccatga tggacgccgt ctacaagaag ttcgccgtcg agcacgggct caagctcggc
     3361 gccccgaccg ccttctcgct gctccgctac gagcgcgaga tcgaccgcct gcaggcctgg
     3421 tgcgacaccc atctcaacac cgtcgtcacg ctgctcaccc acgacaagcg caacatcacg
     3481 cagaagttct tcgaggaagt ggcgatccag gtccgccgcg ccttcgagcg cgccaaccgc
     3541 gacgccgaag tctggctgcg ggcgatcatg gcgccgatgg aaacccaggt tcgcgagcac
     3601 cagatccagt tgaagcgtcg tctggaaagc atcaagcgca tccaccaggc gaccgacacg
     3661 ctggaagacc gcatcgccga actcgacagc gtcggcaaga cgctcgaaga gcaactggcg
     3721 atgctggccg acatcgaata ccgcgtgcgt gacctgatga cgccggaaca gatggccgaa
     3781 ctgcgcgccg cctgaacgcg gcccgcgctc agctaccgaa gccgacgatc tcgcgcggaa
     3841 tctggtcgag cggcaattcg cggccgacac cgccgagctt gaacgcttcc ttcggcatgc
     3901 catagacgac gcaggtcgac tcgtcctcgc ctatcgtcgt cgcgccggcg tcgcgcattt
     3961 ccttcaaacc gcgcgcgccg tcgtcaccca tgccggtcat gatgatgccc agcgcgttgc
     4021 gcccggcgac ctgggccacc gagcggaaca acacatcgac cgacggcttg tgccggttga
     4081 ccagcggccc gtcgcgcacc tcgaccaggt actgggcgcc gttgcggcgc agcatcatgt
     4141 gccgaccgcc cggcgcgatc agcgccagcc cggggtgcac ccggtcgccg tccctggctt
     4201 cgcggatttc catcgcgcac agcccgttca ggcgctcggc gaacatcgcg gtgaatttct
     4261 ccggcatgtg ctggacgatg acgatgccgg tggtcaccgc cggcaggcgg gagagcaccg
     4321 cttcgagcgc ctgcgtgccg ccggtcgagg tgccgatggc gaccacccgg tcggtcgtcc
     4381 cgtacatgcg cgccccctgg tgcggggtgc tggccagcat ggcgtcggcc gactgcttgg
     4441 gcgccaccgc cagcgggctg cgcgcggcgc cggtcatgct gcgcacgacg cgcatgttgg
     4501 cccgcgccgc cgacttgatc gccgagatga tgtcgttgct cgaatcctcg aggaaggatt
     4561 tcaggttgag tttgggcttg gtgatgatgt tgatcgcgcc tgccgccagc gcctccatcg
     4621 tcacctgcgc ccccttgtcg gcgagcgacg agcagatgat caccggcgtc ggccgctcgg
     4681 ccatgatctt cttcaggaag gtcagaccgt ccatgcgcgg catttcgata tcgacgatga
     4741 aaacgtcggg ccactgcgcc ttcagcttgt cgatcgcgaa taccggatcc tgcgcggtgg
     4801 cgatcacctc gatcgagggt tcgcggctga tcgcctgggt aatcacctgg cgcaccagcg
     4861 ccgaatcatc gacgatcatg accttgatct tgttcattgt tgttccttgg aaaaatcctg
     4921 ggcaccgggc aacgccagcc agacatgccc gctggcgaga tcgaaagtca gccgccgccg
     4981 cctggcgcca ccgacgtgct cgcgcgtcac ccgaaagccc tgggcggcca aggcctggcg
     5041 tgttgcctcg acgttgcgtt gaccgacttc catgttacca gcgctgcttc cggcgaacat
     5101 attgccgccg ccgaggagct tgatttggca attggccggc gcaacgcgac gcgaacgcaa
     5161 ctcgtacacg aagcgctcga tcgcctcatc ggcataacga caatccggca acgacgccgg
     5221 cggccgcgcg cggcccggca gcaggatgtg gcacatgccg ccaaaacggt gctgcggatg
     5281 ccagacggtg acggcaacgc aggaaccgag cagggtaccg atgcggagcg gtgccgatgc
     5341 aaaatggaat tcgcccggat ggaggaagac ttccggacgc gcgagacagg aggacatgac
     5401 ggcgactcag cgagcgcgtt gatagatggt cggacgctgg gcaacgaagt cgtcggtgac
     5461 accgaacagc gattccgaat gaccgatcac cagatgcccg tcgcgcttca ggtagggcag
     5521 cagattggtg accaccttgc gcttggtcgg gttgtcgaaa tagatcatca cgttgcgcag
     5581 gaagatcagg tcgaagatgc cgatatcctt gcgcggcgtc agcaggttgt tgtgcaggaa
     5641 acgcacgcgc tggcgcagtt cgcggacgat gagcagggtg ccggcctgtt cgcggatgcc
     5701 tttcaggcag tatttcttga gcatcgccgg cggaatgccg ctgatccggt cgagcgaata
     5761 atgcccctgc tcggctttgc gcagcacgcg cgtgctgatg tcggtgccga ccacttccca
     5821 gggcgcttcg cccagcgtat cggccagcac catggccatc gaataggcct cctcgccgct
     5881 cgacgaggcg gcgctccaca cccggaaagg cgtgccccgc gactggctgg cgcaagtgcg
     5941 caggaaatcg aaatgcttcg gttcgcgaaa gaaataggtt tcgttggtgg tgatcaggtc
     6001 gatcatcaac tgcagttcgc ccgcgttctc cttttcccgg acaaacttgt aatagtcggc
     6061 gaaacgggta aaaccgtagt gctacaggcg tttgccaagg cgaccgacga gcagcacctt
     6121 tttcgcatcc gccaggctga tgccggccag ctcgaaaatc aggcgctgga aattggcaaa
     6181 ctcggcgggc gtgatatcca cgccgtcagg ttgggaactg ggcatcgcgg ctttccgaag
     6241 gtggtgtcga acggctattg tgcacgccgg gcttgccgct gcaaacccgg cggcatgacc
     6301 agggtcaggc gacggcgccg agttcgccct ggttgatggc ggcgatcacc gccacctcgt
     6361 cgagcgacag gatacgctcg acgtcgagca ggacgacgaa acggccgccg accttgccca
     6421 tgccggaaat gaagtcggtg cggatgcgcg tcccgaaggt gggcggcggt tcgatatcgc
     6481 ccggcgcgat ttccagcacc tcggataccg catcgacaac gacccccatc tgttgagcgt
     6541 gggcccccgc ggtctcggca atctcgatga tgacgatgca ggtgcgcttg gtgatttctc
     6601 cggcactgcg gccgaaacgc accgacaggt caatcaccgg cacgacggcg ccgcgcaggt
     6661 tgatgacgcc acggatgaaa ggcggcacca gcggcacctc ggtgatctgg ccgtactcga
     6721 tgatctcccg gatggcgaga atgccgatgg cgaacatttc ctcgccgagc aggaaagtca
     6781 ggtactgctg ctgctcggtg tcgccggcaa cgaccggcgg ctggccggac attgcctgaa
     6841 cgggatgcag gttttgagtc atggcgaact ccccgaccgc tcagaagcgg gcaaagctgc
     6901 tgtcatcgag cgagccggcg aaattggccg gcgccgcttc ggcctgacga cgcccagcgc
     6961 tgcgcgtcgc cggcgtcgat ccgccacgcc ggttggcagt gccacccgca gcccggttgc
     7021 cgaggttgaa gaaggccatc aattcctgca ggttgccggc ctgcgacgac atctcctcgg
//
LOCUS       DAOSDX010000022         7035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3908_length_7035_cov_3.282808, whole genome shotgun sequence.
ACCESSION   DAOSDX010000022 DAOSDX010000000
VERSION     DAOSDX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_7035_cov_3.282808"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PLN02_00920"
     CDS             complement(<1..1246)
                     /locus_tag="PLN02_00920"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HOI50912.1"
                     /translation="MSGTLEFEFRFGKANKGAPSRPSFRILVLADLAGATARRQPLAQ
                     RSPRMVDIDNLAATLADFAPTLTLPASDDHAAPAGTLRFGRLDDFHPDAIYATPAFAA
                     LRASRNALGDPQRSAMTAADLGLAVEAPPRRESDADTLNRLLGGNATRARPVTADIVN
                     RLVRDVVAQHVSSDDRALSPGALAEFDQRIARHMRAILRHPDFRRLESAWRGISHLVS
                     ELAGDDGIQVDLLDLSRDELQADLLAGGKGLRAALADSVGENGPLSLLVADFSFTAAA
                     ADLDTLDALARLAMAADAPCLAAAQPALLGCASVEELADPAQWQPLAPALAERWQTLR
                     RSQAAAWLGLTLPRLLGRLPYGRSTDPVSAFAFEELADGAAHEDFAWENPVYAQARLL
                     GEAYAEDGDEMDISGYLEIDALP"
     gene            complement(1336..2430)
                     /locus_tag="PLN02_00925"
     CDS             complement(1336..2430)
                     /locus_tag="PLN02_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOI50913.1"
                     /translation="MVPLLPKQSILVVDDTPENIDVLRNVLAADYRLRVATSGEKALK
                     IIYSDEPPDLILLDIMMPGLSGLEICRRLKANPDRRRIPVIFVTAMSSIEDEERGLAL
                     GAVDYITKPISPPIVKARVRTHLALYDQARELERMVRQRTQELLTTRQQIIRRLGRAA
                     EFKDNETGNHVLRMSHYARLIGEAHGLGAEAAGIIFQTAPMHDIGKIGIPDAILLKPG
                     KLDAEEWKVMHQHPVMGAEIIGKHDNELLETARVIALSHHEKWDGSGYPHGLRGTDIP
                     LEGRIVAIADVFDALVSVRPYKPAFPLDAALRLMEQQTGSHFDPQLMQAFRQALPDIL
                     RIREIYADENGALTDLEFHIQEIYDQRNDS"
     gene            complement(2442..6722)
                     /locus_tag="PLN02_00930"
     CDS             complement(2442..6722)
                     /locus_tag="PLN02_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI50914.1"
                     /translation="MGEISLRKSGFAHSLVLRTGLLILLALAAFTVGIVRLIGEPTID
                     RLANSQLHLAAEQLEARYTRLLDSVEIALRNSHAWGANGSFDHGDLLRFNEFFFPVLA
                     NNTEINSVIYAHESGREFFLLLDKDGGWTNRISFPAEWGRISYWITWDAERHITGVSV
                     RESDYDPRQRPWFKGAMALTDNRQIHWTQPYIFFTNKAPGLTAAMRWRGRDGAYHVIA
                     HDVRLRDIAQATTGKTIGRDGQASLLLDDGRLLAPPGLPRFASPEAGQQAMLKSAEEL
                     DLGELAQARRQWLDNPMPATGIDRVDRPDGRWYSLFRQLDAGRTGIWLALLAPHRDFV
                     PVSGNDLLVLAAILLAALGLGVAVAVRMARQFGEPLAELTEESHRIGRLDLDTPVIAT
                     APWQEVQELAGALENMRQQLQGGRQAMQDINADLEHTVAMRTGALRQSQEALRQREAF
                     FRAVFDNAAVGIVNLEPDGQPNEINRAFASFIARSEDSLRQEHGLCLAEADNERMQQM
                     LRRVADGEANLRNELEFIDADGNRRWGDVQVSAMRDDQGKLTSLLLTVLDISDRREIE
                     RELIRQFAFLRALLDTIPNPIFYKGEHTRFLGCNRAYEEFFGVDRGHFIGKRVLDLDY
                     LPEDERRAYQEEDERVIAECSRHSRVVTMRRADGSLRDTLYAVSGFRSPDGAPGGLIG
                     VIVDITAQKDAEREAEKARAAAESAAAAKADFLANMSHEIRTPMNAIIGMTHLALQTP
                     LDVRQRNYLNKVDAAAKGLLGIINDILDLSKIEAGMMRFERTPFSLDATLQHLGDLSA
                     LRARERGLELLFDLAPDVPDRLLGDPLRLGQVLLNLVGNAIKFTEKGEVRVEVRLLEA
                     DADKARIRFEVRDTGIGMTPEQLASLFTAFTQADSSTTRKYGGTGLGLSICKRIVDQL
                     GGRIGASSEPGVGSSFVFELPFELASREAETPRRLGLPEHLRTLVVDDSPGAREIFAH
                     MLAGLGIESRAVGSGNDALVELQSANNAGQPYQLLLLDWKMPGMDGAELLAAVRRDFP
                     GAQAPAVVMITAFDQDELHELLEPYSVSAILAKPVTPSSLFDSIMLALHRAPSVPEAP
                     TNVAASTIPSGRHVLLVEDNEVNRELAEEMLRNFGLVVSTAENGAIAVERVRDGAFDL
                     VLMDCQMPVMDGYEATRRIREDLGREDLPVIAMTANALAPDRERCLAAGMDDHVAKPI
                     DVAHLQATLAHWLNGLPAQPIVSAGAAAASAAGELDEETAIARLGGNRQLYQRLLKRF
                     GEDQADFPERLHTARAEHDDAQAILLVHTLRGLAGNIGASHLVSLAAELEHCLRQTPE
                     DGNRIDSLVVRLLDALAAVLAATGGAAEAAAPVSRQMPLPVESLSQLRQLLADDDAAA
                     VRVFEDIEDALRQRLDASAFDPLARAIATYDFETAIDILDRIAATLRQ"
     gene            complement(6819..>7035)
                     /locus_tag="PLN02_00935"
     CDS             complement(6819..>7035)
                     /locus_tag="PLN02_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOI50915.1"
                     /translation="LIPAGPKAGTLSDAASKPIFIAGPEHWRAMADQMGTPLVLRVAS
                     DGRISVTAALDRRLEYLGDRTNINVVP"
BASE COUNT          995 a         2120 c         2671 g         1249 t
ORIGIN      
        1 tgggcagcgc gtcgatttcc aggtagccgg agatatccat ctcgtcgccg tcctcggcgt
       61 aggcttcgcc gagcaaccgg gcctgcgcgt agaccgggtt ttcccaggcg aaatcttcgt
      121 gcgccgcgcc gtcggccagt tcctcgaagg caaaggcgct gaccgggtcg gtactgcggc
      181 cgtagggcag gcgaccgagc aggcgcggca gcgtcagccc cagccaggcg gcggcctggc
      241 tgcggcgcag cgtctgccag cgttcggcga gcgccggcgc cagcggctgc cattgcgcgg
      301 ggtcggcgag ttcttcgacc gaggcgcagc cgagcagtgc gggctgggca gcggccaagc
      361 agggggcgtc ggcggccatc gccaggcgag ccagcgcgtc cagcgtatcg aggtcggccg
      421 ccgcagcggt gaaggaaaaa tcggcgacga gcagcgacag cgggccgttc tcgccgacgc
      481 tatcggcgag tgcagcgcgc aggcccttgc caccggcgag cagatcggct tgcagttcgt
      541 cgcgcgaaag atcgagcaga tcgacctgaa ttccgtcgtc gccggccagt tcgctgacca
      601 gatgcgagat gccgcgccac gccgattcga ggcgccggaa atccgggtga cgcaggatag
      661 cgcgcatgtg ccgcgcgatg cgctggtcga attcggccag cgcacccggg cttagcgccc
      721 ggtcgtcact gctgacgtgc tgggcgacga cgtcgcgcac caggcgattg acgatgtcgg
      781 cggtgaccgg ccgtgcccgc gtcgcgttgc cgccgagcag gcggttcagg gtgtcggcat
      841 cgctttcccg gcgcggcggt gcttcgacgg cgaggccgag gtcggcggcg gtcatcgcgc
      901 tgcgctgcgg gtcgccgagg gcgttgcggc tggcgcgcag cgcggcaaag gccggcgtgg
      961 cgtagatggc gtcgggatgg aagtcgtcga gacggccaaa gcgcagggtg ccggccggtg
     1021 ctgcgtggtc gtcgctggcg ggcagcgtca gggtcggggc gaagtcggcc agcgtcgccg
     1081 ccaggttgtc gatgtcgacc atccgcggcg agcgttgcgc cagcggctga cgccgggccg
     1141 ttgcaccggc caggtcggcc agcaccagga tgcggaagga cggccgggat ggcgcgcctt
     1201 tgttcgcttt gccgaaacgg aattcgaatt ccagagtgcc actcaccttg ttctcccgag
     1261 ggttttgcca aggccgatac tgtacccgag cttgccccgg atatgctccc ggaattacct
     1321 gctggccttg ttgtctcagg aatcgttacg ctggtcgtag atttcctgga tgtggaattc
     1381 gaggtcggtc agcgcgccgt tctcgtcggc gtagatttcc cggatgcgta ggatgtcggg
     1441 cagggcctgg cgaaaggcct gcatcagttg cgggtcgaag tggctgccgg tctgctgctc
     1501 catcaggcgt agcgcagcgt cgagcgggaa ggcgggcttg tacggacgga ccgagaccag
     1561 cgcgtcgaag acatcggcga tggcgacgat gcgtccttcg agcggaatgt cggtgccgcg
     1621 caggccgtgc gggtagccgc tgccgtccca cttctcgtgg tggctgagag caatgacgcg
     1681 ggcggtttcg agcagttcgt tgtcgtgctt gccgatgatc tcggcaccca tcaccgggtg
     1741 ctggtgcatg accttccatt cttcggcgtc gagcttgccg ggtttgagca ggatggcgtc
     1801 ggggatgccg atcttgccga tgtcgtgcat cggcgccgtc tggaagatga tgccggcggc
     1861 ctccgcgcca aggccgtggg cttcgccgat cagccgggcg taatggctca tgcgcaggac
     1921 gtggttgccg gtctcgttgt ccttgaattc ggcggcgcgg ccgaggcggc ggatgatctg
     1981 ctggcgggtg gtgagcagct cctgggtgcg ctgccgcacc atcctttcga gttcgcgcgc
     2041 ctggtcgtag agggcgaggt gggtacggac gcgcgccttg acgatcggcg ggctgatcgg
     2101 cttggtgatg tagtcgacgg cgccgagcgc caggccgcgc tcctcgtcct cgatgctgct
     2161 catcgcggtg acgaagatca ccgggatgcg ccggcggtcc gggttggcct tgaggcggcg
     2221 gcagatttcc aggccggaga ggccgggcat catgatgtcg agcaggatca ggtcgggcgg
     2281 ttcgtcggaa tagatgatct tgagcgcctt ctcgccactg gtggcgaccc gcagccgata
     2341 gtccgcggcc aggacgttgc gcagcacgtc aatgttttcc ggcgtgtcat cgacgacgag
     2401 gatgctttgc ttgggcagca ggggaaccat ggtggctgga ctcactggcg aagggtagcg
     2461 gcgatccggt cgaggatgtc gatggcggtt tcgaaatcgt aggtggcgat ggcgcgggcc
     2521 agcggatcga aagcactggc gtcgaggcgc tgccgcaagg cgtcttcgat gtcctcgaag
     2581 acgcgcacgg cggcggcatc gtcgtcggcc agcaattgcc gtagctggct caggctttcc
     2641 actggcagcg gcatctgccg gctgaccggg gcggcggctt cggcagcgcc gccggtcgcg
     2701 gcaaggaccg cggccagcgc gtcgagcagg cggacgacca ggctgtcgat gcggttgccg
     2761 tcttccggcg tctggcgcag gcaatgttcg agttcggcgg cgaggctgac caggtggctg
     2821 gcgccgatgt tgccggcaag gccgcgcagg gtatggacga ggaggatcgc ctgggcatcg
     2881 tcgtgctcgg cgcgggcagt gtgcaggcgt tccgggaaat cggcctggtc ttcgccgaag
     2941 cgcttgagca ggcgctggta gagttggcgg ttgccaccca ggcgggcgat ggccgtttcc
     3001 tcgtcgagtt cgccggcagc gctcgctgcg gcggcgccgg cggagacgat cggctgcgcc
     3061 ggcaggccgt tcagccagtg ggcgagggtg gcctggagat gggcgacgtc gatcggcttg
     3121 gcgacgtggt cgtccatgcc ggcggccaga cagcgctcgc ggtcgggggc gagcgcgttg
     3181 gcggtcatgg cgatcaccgg caggtcctcg cgcccgagat cctcgcggat gcgccgggtg
     3241 gcctcgtagc cgtccatgac cggcatctgg cagtccatca ggaccaggtc gaaagcgccg
     3301 tcgcgcacgc gctcgacggc gatcgcgccg ttttcggcgg tactgacgac cagtccgaag
     3361 ttgcgcagca tctcctcggc cagttcccga ttgacttcgt tgtcctcgac cagcagcaca
     3421 tgacgcccgc tcggaatggt gctggctgcg acgttggtcg gcgcttcggg gacgctgggc
     3481 gcgcggtgca gggcgagcat gatgctgtcg aacagcgacg acggcgtcac cggtttggcg
     3541 aggatggcgc tgacactgta cggttcgagc agttcgtgca attcgtcctg gtcgaaggcg
     3601 gtgatcatca ccaccgccgg cgcctgtgcg ccggggaagt cgcggcgcac ggcagcgagc
     3661 agttcggcgc cgtccatgcc cggcatcttc cagtcgagca gcagcaactg gtagggctgg
     3721 ccggcattgt ttgcgctctg cagttcgacc agggcgtcgt tgccgctgcc gacggcgcgg
     3781 ctttcgatgc cgagcccggc cagcatgtgg gcgaagatct cgcgggcgcc ggggctgtcg
     3841 tcgacgacca gggtacgcag gtgttcgggc agccccaggc ggcgcggcgt ttccgcttcg
     3901 cggctggcga gctcgaaggg tagttcgaag acgaagctgc tgccgacgcc gggctcgctg
     3961 ctggcgccga tgcggccgcc gagctggtcg acgatgcgtt tgcagatcga caggccgagg
     4021 ccggtgccgc cgtacttgcg ggtggtcgag ctgtcggcct gggtgaaggc ggtgaacagg
     4081 ctggccagtt gttccggcgt catgccgatg ccggtgtcgc gtacttcgaa gcggatgcgc
     4141 gccttgtcgg catcggcttc gagcaggcgg acctcgaccc ggacctcgcc tttttcggtg
     4201 aacttgatgg cgttgccgac caggttgagc agcacctgac cgaggcgcag cgggtcgccg
     4261 agcaggcggt cggggacgtc cggggcgagg tcgaagagca gttccagccc gcgttcgcgg
     4321 gcgcgcagcg cgctcaggtc gccgaggtgc tgcagcgttg cgtcgaggct gaacggcgtg
     4381 cgctcgaagc gcatcatgcc ggcttcgatc ttcgacaggt cgaggatgtc gttgatgatg
     4441 ccgagcaggc ccttggcggc ggcgtcgacc ttgttcaggt agttgcgctg gcggacgtcg
     4501 agcggcgtct gcagcgccag gtgggtcatg ccgatgatgg cgttcatcgg ggtgcggatt
     4561 tcgtggctca tgttggccag gaaatcggcc ttggcggcgg ctgcggattc ggcggcggcg
     4621 cgcgcttttt ccgcctcgcg ctcggcatcc ttctgggcgg tgatgtcgac gatgacgccg
     4681 atcagcccgc ccggcgcgcc gtccggcgag cggaagccgc tcaccgcgta gagcgtgtcg
     4741 cgcaggctgc cgtcggcgcg gcgcatggtg acgacgcggc tgtggcggct gcattcggcg
     4801 atcacccgtt catcctcttc ctggtaggcg cgccgctcgt cttccggcag gtaatcgagg
     4861 tcgaggacgc gcttgccgat gaagtggccg cggtcgacgc caaaaaactc ctcgtaggcg
     4921 cggttgcaac cgaggaagcg ggtgtgctcg cccttgtaga agatcggatt gggaatggtg
     4981 tcaaggaggg cgcgcaggaa ggcgaactgg cggatcagct cgcgctcgat ctcgcggcgg
     5041 tcggaaatgt cgagaacggt gagcagcagc gaggtgagct tgccctgatc gtcgcgcatg
     5101 gcgctgacct ggacgtcgcc ccagcgacgg ttgccgtcgg cgtcgatgaa ctccagttcg
     5161 ttgcgcaggt tcgcctcgcc atcggcgacc cgcctgagca tctgctgcat gcgttcgttg
     5221 tcggcttctg ccaggcagag gccgtgttcc tggcgcaggc tgtcttcgct gcgggcgata
     5281 aagctggcga aggcgcggtt gatctcgttc ggctggccgt cgggttccag gttgacgatg
     5341 ccgaccgctg cgttgtcgaa gacggcgcgg aagaaggctt cgcgctggcg cagggcttcc
     5401 tggctctggc gcagggcgcc ggtacgcatg gccaccgtat gctcgaggtc ggcgttgatg
     5461 tcctgcatgg cctggcggcc gccctgcagt tgctggcgca tgttttccag ggcgccggcg
     5521 agttcctgca cctcctgcca gggcgcggtg gcgatcaccg gcgtgtcgag gtcgaggcgg
     5581 ccaatgcggt ggctttcctc ggtcagttcg gcgagcggtt cgccgaactg gcgggccatg
     5641 cggacggcga cggcgacgcc caggccgagc gcggcgagca ggatggcggc aagcaccagc
     5701 aggtcgttgc cggaaaccgg cacgaaatcg cggtgcggcg cgagcagcgc cagccagatc
     5761 ccggtgcgac cggcgtcaag ttggcggaac aggctgtacc agcggccgtc gggacggtcg
     5821 acgcggtcga tgccggtcgc cggcatgggg ttgtccagcc actggcggcg ggcctgggca
     5881 agttcgccga gatcgagttc ctcggcactc ttcagcatcg cctgctggcc cgcttccggg
     5941 ctggcgaaac gcggcaggcc gggcggcgcc agcaggcggc cgtcgtcgag cagcaggctg
     6001 gcctggccgt cgcgaccgat ggtcttgccg gtggtcgcct gggcgatgtc gcgcaggcgg
     6061 acgtcgtggg cgatcacgtg gtaggcgccg tcgcgtccgc gccagcgcat cgccgcggtc
     6121 agcccgggcg ccttgttggt gaagaagatg tagggctgcg tccagtggat ctgccggttg
     6181 tcggtgagcg ccatggcgcc cttgaaccag gggcgctggc gcgggtcgta gtcgctttcg
     6241 cggacgctga cgccggtgat gtggcgctcg gcgtcccagg tgatccagta gctgatccgg
     6301 ccccattcgg ccgggaagct gatgcggttg gtccagccgc catccttgtc gagcagcagg
     6361 aagaactcgc gtcccgattc gtgggcgtag atcaccgagt tgatttcggt gttgttggcc
     6421 agcaccggga agaagaattc gttgaagcgc agcaggtcgc cgtggtcgaa actgccgttg
     6481 gcgccccagg catggctgtt gcgcagggca atctcgactg aatcaaggag gcgggtgtag
     6541 cgcgcttcca gttgttcggc ggcgaggtgc agttgtgagt tggccagccg gtcgatggtc
     6601 ggttcgccga tcaggcggac gatgccgacg gtgaaggcgg ccagcgcaag caggatgagc
     6661 agcccggtgc gtagcaccag gctgtgggcg aagcccgatt ttcgtaaaga aatctcgccc
     6721 acggcgtttt cccccgacct gcttttgttc gatggctggt gcccggccgt tctgcttgcg
     6781 gcagctcccc gccagcatag ccaagcgggt cggggcgatc aaggcacaac gttgatattt
     6841 gtgcggtcgc cgagatattc gaggcgacgg tcgagggcgg cggtgacgga gatgcggccg
     6901 tcgctggcga cgcgcaggac gagcggcgtg cccatttggt cggccatcgc ccgccagtgc
     6961 tccgggccgg cgatgaagat cggtttcgat gcggcatccg acagcgtgcc ggccttcggc
     7021 ccagccggga tgagg
//
LOCUS       DAOSDX010000023         6983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3943_length_6983_cov_3.387702, whole genome shotgun sequence.
ACCESSION   DAOSDX010000023 DAOSDX010000000
VERSION     DAOSDX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3943_length_6983_cov_3.387702"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2152)
                     /locus_tag="PLN02_00940"
     CDS             complement(<1..2152)
                     /locus_tag="PLN02_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI50916.1"
                     /translation="MKTLQAKPLPARAGELREYMRGLRPDAKRLAEIQSAYDNLALLG
                     QLLCAGTDISAMRQAFGELADSLLAQLADEHFRKAVLGLESSARAAIDVMVRNLYERT
                     ADIGFLATDTEIVAFARNAGSEAGDTGERTHLQARFAEYVDKYTVYHDVVLLSPQGEI
                     LARLDSANPLPRTSDAFVAAALSGERPYLEVFRPTDLLPDVANPLIYACRVSDDDGTP
                     LGVLCLCFRFQDECERIFTNLLGDDDWRVLTLVDGEGRILASSDPWQFPPGARLAMCG
                     DGAPRVARFAGREYLACGRPGQAYQGYAGPGWYGLALAPLEHAFDLSAAHELAAVAED
                     FLACVLETTTLLSSELRRVPREAARIQNDLNRAVWNGHVWLAREPEAAQSGSFAKVLL
                     GEIGNTGVRTRAVFGESINNLYKTVISSALFDASAHAAMAIDIMERNLYERANDCRWW
                     ALTGRFRSALAAAQQGEAPERAPLTATLRQLNSLYTVYTNLILFDQGGRVLAVSNPAY
                     NDCVGRVLDEPWLRPTLALTDSSRYVVSPFRASALYDGRPSYVFAAAVLDPQGREPVG
                     GIALVFDAEPQLQAMLHDALPRAADGRVAAGAFAVFADRQGHVIASSDGELAPGTPLM
                     IGNEFFHIAPGAHYSNVIIYDGRYYAVGSAAGAGYREYPGDREGRSGALLALIFTPLS
                     ERVIDPAMLPEAPEAGAAPYRRHGQGESLDIACFH"
     gene            complement(2314..2652)
                     /gene="sugE"
                     /locus_tag="PLN02_00945"
     CDS             complement(2314..2652)
                     /gene="sugE"
                     /locus_tag="PLN02_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HOI50917.1"
                     /translation="MNAGAAWIVLFFAGLCEVGWAVGLKYTEGFSRLWPSVWTVGLMV
                     ASVVLLGWSLRVLPLGTAYAVWTGIGAVGTAICGMLLFGESREALRLASIALIVAGIV
                     GLKLLTPNPH"
     gene            complement(2649..3932)
                     /gene="hemL"
                     /locus_tag="PLN02_00950"
     CDS             complement(2649..3932)
                     /gene="hemL"
                     /locus_tag="PLN02_00950"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOI50918.1"
                     /translation="MTSRNEELFARAQKHIPGGVNSPVRAFRSVGGTPLFFQKGVGSQ
                     VQDADGKWYTDYVGSWGPMILGHAHPEVIAAVQAAVVDGLSFGAPTEREVDIADLLCE
                     MVPSLDMVRLVSSGTEATMSAIRLARGFTGRDILVKFEGCYHGHADHLLVKAGSGLLT
                     FGNPSSGGVPAGTVETTMVLSYNDPQGLAEAFRTHGDKIAAVILEPVVGNMNLIAPTP
                     EFLKALRELTAQYGAVLIFDEVMTGFRVDLKCAQGLFGITPDLSTFGKVVGGGMPLGA
                     FGGRREIMEKIAPLGPVYQAGTLSGNPIATAAGLATLKLIRAPGFHAALTAKTKALCD
                     GLVAAARKHGVAFAAQNVGGMFGLYFAERCPGTYDEVLACDKDAFNRFFHAMIDAGHY
                     FAPSAFEAGFVSAAHSDAEIAATVAAADAWFAQQS"
     gene            complement(3919..4563)
                     /gene="thiE"
                     /locus_tag="PLN02_00955"
     CDS             complement(3919..4563)
                     /gene="thiE"
                     /locus_tag="PLN02_00955"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOI50919.1"
                     /translation="MAQAELHGLYAVTPDDLPRETLLTGVAAALAGGCRIVQLRDKTG
                     PMPERLARAHALRRLTRDHGARLIINDDLALCQLVDADGLHLGRDDGDLRVARAILGP
                     QRILGASCYADLDSARAAVAAGVDYVAFGTAFPSPTKPDAVRAPLELYARARAELPVA
                     VCAIGGITATNAAPLLDAGVDLLAVITDLFSAPDITARAAQYQRLFEKARHDLA"
     gene            complement(4550..5398)
                     /locus_tag="PLN02_00960"
     CDS             complement(4550..5398)
                     /locus_tag="PLN02_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HOI50920.1"
                     /translation="MPIVSANLPQVLVFAASDPTGGAGIQADLLTLASLGCHPLSALT
                     ALTVQDTSGVIAMQPVAAELLEQQARTVLEDMPVAAFKIGLIGSVENVLAIAEIVGDY
                     PEIPLVFDPVLASGRGDALSGEEIIAAMREMLLPQTTIITPNAPEARRLAESDEDENE
                     PALDVCAARLIEMGAQYVLITGTHESTPEVINTLYGPDGVIRHDRWERLPGSYHGSGC
                     TLASAIAGCLAGGASVEEAVRDAQDYTWQALRHGFRGGMGQFIPDRLFWAREDDEAKE
                     AGDGAS"
     gene            5617..5991
                     /locus_tag="PLN02_00965"
     CDS             5617..5991
                     /locus_tag="PLN02_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI50921.1"
                     /translation="MVIDDSNTIRRSAEIFLVQAGCQVVLAEDGFDALAKIADHQPAV
                     IFCDIMMPRLDGYQTCALIKKNPRFKATPVIMLSSKDGLFDRARGRMVGSDQYLTKPF
                     TKDSLLQTVASFALPAEPEIKP"
     gene            6006..6371
                     /locus_tag="PLN02_00970"
     CDS             6006..6371
                     /locus_tag="PLN02_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI50922.1"
                     /translation="MPVKNILVVDDSPTERFFAVDLLTRAGYLVATAENGEEGIAKAK
                     ATKPDLILMDVVMPGLNGYQATRTLTRDEETKHIPVIVCTSKGQETDKIWGLRQGAVD
                     YLVKPLNPEELLQRIAALP"
     gene            6377..6889
                     /locus_tag="PLN02_00975"
     CDS             6377..6889
                     /locus_tag="PLN02_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI50923.1"
                     /translation="MARKSSLRDFQTYLAGRLSDAAQGHGGASWLGLEIGDDGWLIDL
                     ADGGEIVQAPQLAGVPLVRPWFSGIANVRGNLYAVTDFAAFLGARPTPRNANARLLLV
                     GVRHGSNAALLVPRMLGLKKPENFAEQAADEAFPAWGRRRYVDEDGRLWRKLSVRELL
                     ADPDFMNIGL"
BASE COUNT         1161 a         2163 c         2504 g         1155 t
ORIGIN      
        1 tgtggaagca ggcgatgtcc aggctctcgc cctggccatg gcgccggtag ggggcggcgc
       61 ccgcctcggg cgcttcgggc agcatggccg gatcgatcac ccgttccgac aacggcgtga
      121 agatcagcgc cagcagcgcg ccgctccgtc cctcgcggtc gcccggatat tcgcggtagc
      181 cggcgccggc cgccgaaccg accgcgtagt agcggccgtc gtagatgatc acgttgctgt
      241 agtgcgcgcc gggggcgata tggaagaact cgttgccgat catgagcggc gttcccggcg
      301 ccagttcgcc gtcgctgctg gcgatgacat gcccctggcg gtcggcgaag acggcgaagg
      361 cgccagcggc gacccggcca tcggcggcgc gcggcagcgc gtcgtgcagc atcgcctgta
      421 actgcggctc agcgtcgaac accagggcga tgccgccgac cggttcccgg ccttgcggat
      481 caagcaccgc agcggcgaac acatagcttg gacggccgtc gtagagggcg ctggcgcgga
      541 agggcgaaac cacgtagcgg ctgctgtcgg tcagcgccag cgtcgggcgc agccagggtt
      601 cgtcaaggac gcggccgacg cagtcgttgt aggccgggtt ggataccgcc agtacccggc
      661 cgccctggtc gaacaggatg aggttggtat agaccgtgta aagactgttg agctggcgca
      721 gggtcgcggt cagcggcgcc cgttccggcg cctcgccctg ctgggcggcg gccagtgcgc
      781 tgcggaagcg gccggtcagt gcccaccagc ggcagtcgtt ggcccgttcg tagagattgc
      841 gctccatgat gtcgatggcc atcgccgcgt gcgccgaagc gtcgaacagc gccgacgaga
      901 ttaccgtctt gtacaggttg ttgatcgatt cgccgaacac cgcccgtgtc cgcacgccgg
      961 tattgccgat ctcgccgagc aggaccttgg cgaagctgcc gctctgcgcc gcctcgggct
     1021 cccgtgccag ccagacatgg ccgttccaga ccgcccggtt gaggtcgttc tggatgcgcg
     1081 ccgcctcgcg cgggacgcgg cgcagttcgg aggaaagcag ggtggtcgtt tccagcacgc
     1141 aggccaggaa atcctccgcc acggccgcca gttcgtgggc cgccgaaagg tcgaacgcat
     1201 gctccagcgg ggccagcgcc aggccgtacc aaccggggcc ggcatagccc tggtaggctt
     1261 ggcccggccg accgcaggcg aggtattcgc gaccggcgaa acgggcaacg cgcggggcgc
     1321 catcgccgca catggcgagc cgggcgcccg gaggaaattg ccaggggtca ctgctggcca
     1381 ggatgcgacc ttcgccgtcc accagcgtga gcacccgcca gtcgtcgtcg cccagcagat
     1441 tggtgaagat acgctcgcat tcgtcctgga agcggaagca gaggcagagg acgccgagcg
     1501 gtgtgccgtc gtcgtcgctg acccggcagg cgtagatcag cgggttggcg acatcgggca
     1561 gcaagtcggt ggggcggaag acctcgaggt aggggcgctc gccgctcagt gcggcggcga
     1621 cgaaggcgtc gctggttcgg ggtagcggat tagcgctatc gagacgtgcc aggatctcgc
     1681 cttgcggaga aagcaggacg acgtcgtgat agaccgtgta tttgtcgacg tattcggcaa
     1741 agcgcgcctg cagatgcgta cgctcaccag tgtcgcctgc ctcgctgccg gcgttgcggg
     1801 caaaggcgac gatttcggtg tcggtggcca ggaaaccgat atcggcggtg cgctcgtaga
     1861 ggttgcgcac catgacgtcg atcgcggcgc gggccgagga ttccaggccg agcaccgcct
     1921 tgcggaagtg ttcgtcggcg agttgtgcga gcaggctgtc ggccagttcg ccgaaagcct
     1981 ggcgcatcgc cgagatgtcg gtgccggcgc agagcagttg gccgagcagg gccaggttgt
     2041 cgtaggccga ctggatctcg gccaggcgct tggcgtccgg ccgcagtccg cgcatgtact
     2101 cgcgcaattc cccggcgcgg gcgggcagcg gtttggcttg cagtgttttc atggtttccc
     2161 ttctgctgtt ctgcatgcgc accacgaggg cgcgacatgt ggattcagca aaaagggtgc
     2221 cgggcggaaa acgcacggaa atggtgaaaa ttgcgacctg cagccagcgc aacgggcgcc
     2281 gcaacgaggc gcttggcacc agaatggtgc tggctagtgc gggttcggcg tcagcagctt
     2341 gaggccgacg atgccggcga cgatcagcgc gatgctggcc aggcgcagcg cctcgcgcga
     2401 ttcgccgaac agcagcatgc cgcagatcgc ggtgccgacc gcgccgatac cggtccacac
     2461 cgcgtaggcg gtacccaatg gcaggacgcg cagcgaccag ccgaggagga cgacgctggc
     2521 caccatcagg ccgaccgtcc atacgctcgg ccacaggcgc gaaaagccct cggtgtattt
     2581 cagcccgacc gcccagccga cctcgcagag accggcgaag aagaggacga tccaggcggc
     2641 gccggcgttc atgattgctg ggcgaaccag gcgtcggcag cggcgacggt cgccgcgatc
     2701 tcggcgtcgc tgtgcgcggc cgagacgaaa ccggcttcga aggccgacgg cgcgaagtag
     2761 tggccggcgt ctatcatggc gtggaagaag cggttgaagg cgtccttgtc gcaggccagc
     2821 acttcgtcgt aagtacccgg gcagcgctcg gcgaagtaga ggccgaacat gccgccgacg
     2881 ttctgggcgg cgaaggcgac accgtgtttc ctggcggcgg cgaccaggcc gtcgcacagc
     2941 gccttggtct tcgccgtcag cgccgcgtgg aagcccggcg cccggatcaa cttcagcgtc
     3001 gccagaccgg cggccgtggc gatcgggttg ccggacagcg tgccggcctg gtagaccggg
     3061 ccgagcgggg cgatcttttc catgatttcg cggcgaccgc cgaaggcgcc cagcggcatg
     3121 ccgccgccga ctaccttgcc gaaggtggac aggtccgggg tgatgccgaa caggccctgc
     3181 gcgcacttca ggtcgacgcg gaagccggtc atcacttcgt cgaaaatgag cacggcgccg
     3241 tactgggcgg tcagttcgcg cagtgctttg aggaattccg gggtcggcgc gatcaggttc
     3301 atgttgccga ccaccggctc gaggatgacg gcggcgatct tgtcgccgtg cgtcctgaac
     3361 gcttcagcca ggccctgcgg gtcgttgtag ctcagtacca tggtggtttc gacggtgccg
     3421 gccgggacgc cgcccgacga cgggttgccg aaggtgagca ggccggagcc ggccttgacc
     3481 agcaggtggt cggcgtggcc gtggtagcag ccctcgaact tgaccaggat gtcgcggccg
     3541 gtgaaaccgc gcgccaggcg gatcgcgctc atcgtcgcct cggtgccgga cgaaaccagg
     3601 cgcaccatgt cgagcgacgg caccatctcg cacagcaggt cggcgatgtc gacttcgcgc
     3661 tcggtcggcg cgccgaagga gagaccgtcg accaccgcgg cctgcacggc ggcgatgact
     3721 tccgggtggg cgtggccgag gatcatcggc ccccaggagc cgacgtagtc ggtgtaccac
     3781 ttgccgtcgg cgtcctgcac ctggctgccg acacccttct ggaagaacag cggcgtgccg
     3841 ccgacagagc ggaaggcgcg caccggcgaa ttgacgccgc cggggatgtg tttctgggca
     3901 cgggcaaaga gttcttcgtt acgcgaggtc atgtcgggct ttctcgaaaa gtcgttggta
     3961 ttgggcggca cgggcggtga tgtccggcgc cgagaagagg tcggtgatga cggccagcag
     4021 gtcgacgccg gcatcgagca gcggcgccgc gttggtggcg gtgatgccgc cgatggcgca
     4081 gacggcgacc ggcagttcgg cccgcgcccg ggcatagagt tccagcggcg cgcggacggc
     4141 gtccggcttg gtcggcgacg ggaaggcggt gccgaaggcg acatagtcga cgccggccgc
     4201 gacggcggca cgggccgagt cgaggtcggc gtagcaggag gcgccgagga tgcgttgcgg
     4261 gccgaggatg gcgcgggcga cccggaggtc gccgtcgtca cgtccgaggt gcaggccgtc
     4321 ggcatcgacc agttggcaga gtgcgaggtc gtcgttgatg atcagtcgcg cgccgtggtc
     4381 gcgggttagc cggcgcagcg cgtgggcgcg ggcgagacgt tccggcatcg gccccgtctt
     4441 gtcacgcagt tggactatac ggcagccgcc agccagggcg gcggcgacgc cggtgagcag
     4501 ggtttcccgc ggcaggtcgt cgggggtgac cgcgtagagg ccgtgcagtt cagcttgcgc
     4561 catcgccggc ctccttggct tcgtcgtctt cccgggccca gaacaggcgg tccgggatga
     4621 actggcccat gccgccgcgg aaaccgtggc ggagcgcctg ccaggtgtag tcctgggcgt
     4681 cgcgtaccgc ttcctcgacg ctggcgccgc cggccaggca gccggcgatc gccgaggcca
     4741 gcgtgcagcc cgagccgtgg tagctgccgg gcaggcgttc ccagcggtcg tggcggatca
     4801 cgccgtcggg gccgtacagg gtgttgatca cctccggcgt gctttcgtgg gtgccggtga
     4861 tcagcacgta ctgcgcgccc atttcgatca ggcgggcggc gcagacgtcg agtgccggtt
     4921 cgttctcgtc ctcgtcgctt tcggcgaggc ggcgcgcttc gggcgcgttg ggggtgatga
     4981 tcgtcgtttg cggcaacagc atctcgcgca tggcggcgat gatttcctcg ccggacagcg
     5041 catcgcctcg cccagaagcc agcaccgggt cgaagacgag cgggatttcc gggtagtcgc
     5101 cgacgatctc ggcaatcgcc agcacgttct cgacgctgcc gatcaggccg atcttgaagg
     5161 cggccaccgg catgtcctcg agcacggtgc gcgcctgctg ttcgagcagt tcggcggcga
     5221 ccggttgcat ggcgatgacg cccgaggtgt cctgcacggt cagcgcagtg agcgcgctca
     5281 gcgggtggca gccgaggctg gccagggtca gcaggtcggc ctggatgccg gcgccgccgg
     5341 tcgggtcgct ggcggcgaaa acgagaactt gcggcagatt ggcggaaaca atgggcatgg
     5401 gcgggtgtgg cgcggatgtc cgggcgcggg ctggaaagcg cgattttatc cgaatacgct
     5461 gtactgccgc gggattctgg gcttatgctt agcgacttcc ggttggcgat tgttatccgt
     5521 tccgtagtaa tatgttcgcc atatgctaag tgcgccaaga gacactcggg gagctgggaa
     5581 ttgactgatt tatcgagact gcgcggtatc cgtgtcatgg tcatcgacga cagcaacacg
     5641 atacgccgca gtgccgagat ttttctcgtc caggccggtt gccaggtcgt tcttgccgag
     5701 gatggtttcg acgccctggc caagatcgcc gatcatcaac cggcggtcat tttctgcgac
     5761 atcatgatgc cgcgcctcga cggttaccag acctgcgcgc tgatcaagaa gaatccccgt
     5821 ttcaaggcca cgccggtgat catgctttcc tccaaggatg gcctgttcga ccgggcgcgc
     5881 ggtcgcatgg tcggttccga ccaatacctc accaaacctt tcaccaagga tagcctgctg
     5941 cagactgtcg ccagtttcgc attgccggcc gaaccggaaa tcaaaccgta gtccagggag
     6001 caacaatgcc cgtgaagaac attctcgttg tcgacgactc tccgacagag cgttttttcg
     6061 ccgtcgacct gctgaccaga gcgggttatc tggtggctac cgctgaaaat ggcgaagaag
     6121 ggatcgccaa ggccaaggcg accaagcccg acctgatcct gatggatgtc gtgatgccag
     6181 gcctcaacgg ctaccaggcg acgcgcacgc tgacccgcga cgaggaaacc aagcatatcc
     6241 cggtcatcgt ctgtacctcg aagggtcagg aaaccgacaa gatctggggg ctacgccagg
     6301 gagcggtgga ttatctggtc aagccgctca atccggaaga actcctgcaa cgcatcgccg
     6361 ccctgccgta agcgatatgg cccgcaagag cagtctgcgc gatttccaga cttacctggc
     6421 cggacgcctc agcgacgcgg ctcagggaca tggcggtgcc tcatggctcg ggttggagat
     6481 cggcgatgac ggctggctga tcgatctcgc cgacggcggg gaaatcgtcc aggcgccgca
     6541 actggccggc gtgccgctgg tccgcccgtg gttttccggg atcgccaacg tgcgcggcaa
     6601 tctgtatgcc gtgaccgatt ttgccgcctt ccttggcgcc cggccgacgc ccaggaatgc
     6661 caatgccagg ctgttgctgg tcggggttcg ccatggttcg aacgcggcac tgctggtgcc
     6721 gcgcatgctg ggcctgaaga agccggagaa ttttgccgaa caggctgccg acgaagcgtt
     6781 cccggcatgg gggcggcgac gctacgtcga tgaagatggg cgcctctggc gcaagttgtc
     6841 ggtgcgcgag ctgcttgccg atccggattt catgaatatc ggactatgaa aaaggggtcc
     6901 cgccggggcg gggtcgagtg gaggtaagac atggctttag gcttcaagct cccgaaaatc
     6961 gggtcatcca ggaaaaacga ggc
//
LOCUS       DAOSDX010000024         6941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_3974_length_6941_cov_3.470229, whole genome shotgun sequence.
ACCESSION   DAOSDX010000024 DAOSDX010000000
VERSION     DAOSDX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_6941_cov_3.470229"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLN02_00980"
     CDS             complement(<1..861)
                     /locus_tag="PLN02_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOI50924.1"
                     /translation="MSRLPSNSLAYALRQAARIDAAVFAGQSLADNLLARVEAVARPA
                     VQDLVYGSLRAYGRGDFFLARLLERPLAVDEVRALLLVALYRLETRPEAVHTVVDQAV
                     SAAGEIAHGKFRALVNGVLRSFLRQRVALEAALQADPVAPNQHPAWWLQELQTAWPEQ
                     WSTIVAAGNQAPPMALRVNRRCCSRADYQAQLAAAGIAASPLGDDGLTLAQPVPVDRL
                     PGFFDGLVSVQDPGAQRAAELLAPAPGSRVLDACAAPGGKTAHLLERADLDLLALDLK
                     PARCRRIEDGL"
     gene            895..1794
                     /locus_tag="PLN02_00985"
     CDS             895..1794
                     /locus_tag="PLN02_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOI50925.1"
                     /translation="MRPLLIFLLTVLASPDVLAWNAAGHRLSALIAWQGMAPETRACV
                     DAALRHHPDHGRWREKAGADDSALIFAEASTWPDSIRHDPRFAEEAATSPLPGFPDIQ
                     RHSDWHYVDFDHLGKRGRGQLDRQIVALSETVRTTPDIARVAWALPWLAHLVADLHQP
                     FHAGYPADHGGNSLLVEDPADARRPFVSLHTWWDDLPGKSGLRGKRLRQKAGALLAGQ
                     VPPQQGNEALWLRESRALLPSLYPENAGSVAQLVDATYQARAREIADRRISEAGWRLA
                     GLLDAICRQRVSHETPREFRRQQ"
     gene            complement(1778..2701)
                     /gene="fmt"
                     /locus_tag="PLN02_00990"
     CDS             complement(1778..2701)
                     /gene="fmt"
                     /locus_tag="PLN02_00990"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOI50926.1"
                     /translation="MKLIFAGTPEFAAQALQAIVDAGHEVVLVLTQPDRPAGRGMALQ
                     PSAVKRVALAHGIEVFQPATLRDADAQARLAACAAEAMVVAAYGLILPQAVLDLPRHG
                     CLNIHASLLPRWRGAAPIQRALLAGDAQTGVCIMQMEAGLDTGPVLLHEALPIAGDDT
                     TASLHDKLADCGARLIVEALARLPLPAMPQPAEGVTYAHKIEKADAAIDWTKPAVELD
                     RHLRAFNPFPGAQAQLAGQTVKLWAASPCAGSGRPGEILAIDRQTLVVACGEGALAIR
                     ELQKAGGKRLPVQQFLAGNALRVGDLFTAAA"
     gene            complement(2698..3201)
                     /gene="def"
                     /locus_tag="PLN02_00995"
     CDS             complement(2698..3201)
                     /gene="def"
                     /locus_tag="PLN02_00995"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOI50927.1"
                     /translation="MALLPILRFPDSRLRKVAAPVTKIDDRIRRLAADMAETMYEAPG
                     IGLAATQVDVHKRLVVIDVSEDKSQLRVFVNPEVESAEGLALGEEGCLSVPGIYDKVE
                     RAERVRVRYLDLDGNAQVLTAEGLLAVCIQHEIDHLDGKVFVDHLSQLKQTRIKSKLA
                     KQARVTA"
     gene            3377..4366
                     /locus_tag="PLN02_01000"
     CDS             3377..4366
                     /locus_tag="PLN02_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOI50928.1"
                     /translation="MTAICATAAEPLELVANPPDRHVVVKGDTLWAISGKFLKKPWRW
                     PEIWRLNKDQIKNPHLIYPGDVVWLDLSDGNPRLRLGKPLSGRLQPKVYESAASGNIS
                     SIPANAIEPFISQPLIISEEDEKAAARIVATEEDRVVLGNGDTAYVSGIPDASIEKWH
                     VFRPGKPLTDPETGKVVAHEAFFLGNARLTRPGEPATVRITMAKEEVTRGDWLKPAPE
                     QNIVAYVPHPPEVEVRAQIMSIYGAINEGGAGSVVTINRGEADGIEHGHVVALYRKRT
                     ALHLDADIKREETTIPEERYGLAFVFRTFDHVAYALVIESSKSVIVGDAARNP"
     gene            4363..5433
                     /gene="dprA"
                     /locus_tag="PLN02_01005"
     CDS             4363..5433
                     /gene="dprA"
                     /locus_tag="PLN02_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOI50929.1"
                     /translation="MIDTQGLTAWLRLALIPGIGSETQRKLLAAFGLPEAVFAASPSK
                     LRSVIGSKLELLLDFPLGDAIERHLDWAGQPNQHILTLADPAYPGQLLNIADPPTLLF
                     VRGDPSLLQRPALGIVGSRNATPQGLRNARQFSAALAERGLSIVSGLALGIDTAAHHG
                     ALTAGGATIAVIGTGADRVYPARNRDLALSIVERGAIVSEFPLGTPPIAANFPRRNRI
                     IAGLSLGVLVVEAAVESGSLITARQAGEQGREVFAIPGSIHAPQSRGCHRLIREGAKL
                     VETANDILEELGNPPGQSATPPTDEREPALLAVIGHDPCNFDELVERSGMPADVLHGE
                     LLTLELAGRIGILPGNRYQRID"
     gene            5513..>6941
                     /locus_tag="PLN02_01010"
     CDS             5513..>6941
                     /locus_tag="PLN02_01010"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOI50930.1"
                     /translation="MSKKLIIAEKPSVASDIAKALGGFTKHDEYFENDEYVVSSAVGH
                     LLELACPEEFEVKRGKWSFAHLPVIPPHFALNPIEKSAPRLRVLNKLIKRKDVTGLIN
                     ACDAGREGELIFNYIVQHAKAGKPVQRLWLQSMTPQAIRDGFARLRPGSEMQGLADAA
                     VCRSESDWLVGINGTRAMTAFNSKTGGFHLTTVGRVQTPTLAIVVERERKIRDFVPRD
                     YWEVEAEFAAKAGGYTGKWFDEGFKGKQDDEHARADRLWEVARAEAIKAATSGKPGIV
                     SEEAKPETRLSPLLFDLTSLQREANSRFGFSAKTTLGLAQALYEKHKVLTYPRTDSRC
                     LPEDYLATVKETMRVLTGEGAGKGHDEVLLARYAPFAHQILARNWVLPNKRIFNNAKV
                     SDHFAIIPTPQAPKNLNEAEQKLYDFVVRRFLSVFFPAAEYLVTTRITRVEGHPFKTE
                     GKVLVNPGWLAVHGKEGQTGDEGNLV"
BASE COUNT         1311 a         2409 c         2118 g         1103 t
ORIGIN      
        1 gagaccatcc tcgatgcggc gacagcgggc gggtttgaga tcaagcgcca gcaggtcgag
       61 atcggcccgc tccagcaggt gcgccgtctt gccgccgggt gcggcacagg catcgagcac
      121 ccggctgccc ggcgccggcg ccagcagttc ggcggcacgc tgcgcaccag gatcctggac
      181 cgacaccagg ccatcaaaga agccgggcag gcgatcgacc ggcacgggct gtgccagcgt
      241 cagtccatcg tcgccgagcg gactggcggc aattcccgcc gccgccagtt gcgcttggta
      301 gtcggcacgg ctgcaacacc ggcgattgac ccgcagcgcc atcggcggcg cctggttgcc
      361 ggcggcgacg atggtcgacc attgttccgg ccaggccgtc tgcagttcct gcaaccacca
      421 cgccgggtgc tggttcgggg ccaccggatc ggcctgcaag gccgcttcca gagccacccg
      481 ctggcgcaga aaactgcgca acacgccatt gaccagggcg cggaacttgc catgggcgat
      541 ttcgccggca gccgaaaccg cctgatcgac gacggtatgc accgcctccg gccgggtttc
      601 gagccggtac agggcaacca gcagcaaggc tcgcacttcg tccaccgcca gcggacgctc
      661 aagcaggcgg gcgaggaaga agtcgccccg gccgtaagcg cgcaggctgc cataaaccag
      721 gtcctgcacc gccgggcgcg ccaccgcctc gacccgcgca agcagattgt cggccaggct
      781 ctggccggca aaaaccgccg catcgatgcg agccgcctgg cgcaaggcgt aggccagtga
      841 attggaaggc aaacgtgaca tggcccggat tatcgcacgg ggcataattc cggcatgcgt
      901 cctttgttaa tcttcctgct caccgtcctc gccagccccg atgtgctcgc ctggaacgcc
      961 gccggccacc gtctgagcgc actcatcgcc tggcaaggaa tggcgccgga aacccgcgcc
     1021 tgcgtcgacg cagcgctgcg tcaccacccc gaccacggcc gctggcggga aaaggccggc
     1081 gccgacgaca gcgcgctgat ctttgccgag gccagcacct ggcctgacag catccgccac
     1141 gatccacgct ttgccgagga ggcggccacc tccccgctac cgggctttcc cgacatacag
     1201 cggcacagcg actggcatta cgtcgatttc gatcacctgg gcaaacgtgg ccgcggacaa
     1261 ctcgatcgcc agatcgtcgc cctgagcgaa accgtgagaa cgacgccgga cattgcaaga
     1321 gtcgcctggg ccctgccctg gctggcgcat ctggtggccg acctgcacca acccttccat
     1381 gccggctacc ccgcagacca cggcggcaac agcctgctgg tggaagaccc tgctgacgca
     1441 cgccgcccct ttgtcagcct gcacacctgg tgggatgacc tgccgggcaa gtcgggactg
     1501 cgcggcaaac gcctgcggca aaaagccggt gccctgctcg ccgggcaggt gccaccgcaa
     1561 cagggcaacg aggcgctttg gttacgcgaa agccgcgccc tgctgccttc gctctacccg
     1621 gaaaacgctg gcagcgtggc acagctggtc gatgccacct atcaggcgcg ggcccgagag
     1681 attgccgacc gacggatcag cgaagccggc tggcgacttg ccggcctgct cgacgccatc
     1741 tgccgccagc gggtttcaca tgaaacgccg cgggaattca ggcggcagca gtgaacaagt
     1801 cgcccacgcg cagcgcattg ccggccagga attgctgtac cggcagacgc ttgccgccgg
     1861 ccttctgcag ttcccggatc gccaatgcgc cctcgccaca ggcgacgacc agggtctggc
     1921 gatcgatcgc caggatttcg ccgggacggc cgctgccggc gcaagggctg gccgcccaca
     1981 gcttgacggt ctgcccggcg agctgggcct gggcgccggg gaagggattg aaggcgcgca
     2041 ggtggcgatc cagttcgacc gccggcttcg tccagtcgat cgcggcgtcc gccttttcga
     2101 tcttgtgcgc gtaggtgacg ccttctgccg gctgcggcat cgccggcagg ggcagccggg
     2161 ccagcgcctc gacgatcagc cgggcgccac agtcggccag cttgtcgtgc aggcttgccg
     2221 tcgtgtcatc gccggcgatc ggcaaggctt catgcagcag gaccgggccg gtatccaggc
     2281 cggcctccat ctgcatgatg cagacgccgg tctgcgcatc acccgccagc aaggcgcgct
     2341 ggatcggcgc cgcgccgcgc cagcgtggca gcagcgaggc atggatgttg aggcagccgt
     2401 ggcgcggcaa atcgagcacc gcctgcggca ggatcagccc gtaggctgca acgaccatcg
     2461 cttccgcggc gcatgccgcc agccgagcct gggcatcggc atccctcaag gttgccggct
     2521 ggaagacctc gatgccatgc gccagcgcca cccgcttcac cgccgacggc tgcaaggcca
     2581 tgccgcgtcc ggctggccgg tcgggctggg tcaggaccag caccacctcg tgcccggcgt
     2641 caacgattgc ctgcagcgcc tgtgccgcga actccggcgt tcctgcgaaa atcagcttca
     2701 tgcggtgaca cgcgcctgtt tggcaagctt gctcttgatc cgcgtctgct tgagctggga
     2761 aagatggtcg acaaaaacct tgccgtcgag atgatcgatc tcgtgctgga tgcacaccgc
     2821 gagcagtcct tccgcggtca gcacctgggc gttgccgtca aggtccaggt agcggacacg
     2881 cacccgctcg gcccgttcga ccttgtcgta aattccggga accgaaagac agccctcctc
     2941 gcccagcgca agaccttccg cgctctcgac ctcgggattg acgaatacgc gtagctggct
     3001 cttgtcttcg gaaacgtcga tcaccaccag ccgcttgtgc acatcgacct gcgtcgcagc
     3061 aaggccgatg cccggtgctt catacatggt ctccgccatg tcggcagcaa gtcggcgaat
     3121 gcgatcgtca attttcgtga ccggtgccgc gactttacgc aagcgggaat cgggaaaacg
     3181 caaaatgggt aatagtgcca tggaaaacag ggtgaaaatg cttgcttgct tagaggatta
     3241 catgcagaat ctaaagcaat tctcgagata tgaacgtgac ttgcagaggc tatttggacc
     3301 aacgaatctg cagtgcgttc caccggtacg tgagggatta cccatgcttc gcattatatc
     3361 cgcgctgatc ctggctgtga cagctatctg tgcaacggcc gccgagccgc tggaactggt
     3421 tgccaacccg ccggaccgcc atgtcgtagt caaaggcgac acattgtggg ccatttccgg
     3481 gaaattcctg aaaaaaccct ggcgctggcc ggaaatctgg cgtctcaaca aggaccagat
     3541 caagaatccg cacctgattt atcccggcga cgttgtctgg ctcgacctct ccgatggcaa
     3601 tccccgtctg cgcctgggca aaccgctttc cggccggctt cagcccaagg tttatgaaag
     3661 cgcagccagc ggcaacattt ccagcatccc ggcaaacgcc atcgagccct tcatctcgca
     3721 accgctgatc atctcggaag aggacgaaaa ggccgctgcc cgcatcgtcg ccaccgaaga
     3781 agaccgcgtc gtcctcggca atggcgacac cgcctacgtc agtggcatcc cggatgccag
     3841 catcgaaaaa tggcatgtct tccgcccggg caagccgctg accgatccgg aaaccggcaa
     3901 ggtcgtcgcc cacgaagcct tcttcctcgg caacgcccgc ctgactcgtc cgggtgagcc
     3961 ggcaacggtg cgcatcacca tggccaagga agaggtcacc cgcggcgact ggctgaagcc
     4021 ggcccccgag cagaatatcg tcgcctacgt gccacatccg ccggaagtcg aagtgcgcgc
     4081 ccagatcatg tcgatctatg gtgccatcaa cgaaggcggt gccggctcgg tggtcacgat
     4141 caaccgcggt gaagccgacg gtatcgagca cggtcatgtc gtggctctgt atcgcaaacg
     4201 cactgccttg cacctggacg ccgatatcaa gcgggaggaa accacgattc cggaagaacg
     4261 ttacggtctg gccttcgttt tccgcacctt cgatcatgtc gcctacgcac tggtcatcga
     4321 atcctcgaag tcggtaatcg tcggcgacgc agcccgcaat ccgtgatcga cactcaggga
     4381 ctgaccgcct ggttgcggct agccctgatt cctggcatcg gcagcgagac gcaacgcaag
     4441 cttctcgctg ctttcggttt acccgaggcc gttttcgccg ccagcccgag caagctgcgc
     4501 agcgtcatcg gcagcaagct cgaactgctt ctggatttcc cgcttggcga cgccatcgaa
     4561 cgccacctcg actgggccgg acagccgaat caacacatcc tgactctggc cgaccctgcc
     4621 tatcccggcc agctactcaa cattgccgac ccgccaacgc tgctcttcgt gcgcggcgat
     4681 ccctcgctac tccagcgccc ggccctgggc atcgtcggca gccgtaacgc aacgccccag
     4741 ggactgcgca acgcaaggca gttttccgcc gcgctggccg aaagaggact gagcatcgtc
     4801 agcggcctgg cgctgggcat cgacaccgcc gcgcaccacg gcgcgctgac cgccggaggc
     4861 gccaccatcg ccgtcatcgg caccggtgcc gaccgggtct acccggcacg caatcgtgac
     4921 ctggcgctga gcatcgtcga acgcggcgca atcgtttccg agttcccgct cggaacccca
     4981 ccaatcgctg ccaacttccc gcgccgcaac cgcatcatcg ccggcctctc gctcggcgtc
     5041 ctggtcgtcg aagccgccgt cgagagcggt tccctgatca ccgcccgcca ggccggcgaa
     5101 cagggacgtg aagtcttcgc catcccgggc tccatccatg cgccgcagtc acgcggctgc
     5161 catcgcctga tccgcgaagg cgccaaactg gtcgagacgg caaatgacat tctcgaggaa
     5221 cttggtaacc cgccaggcca aagcgcaacg ccacccaccg acgaacgcga accggcattg
     5281 ctggccgtga tcggccacga cccctgcaac ttcgatgaac tggtcgagcg cagcggcatg
     5341 cctgccgacg ttctgcacgg cgaactgctg acgctggagc tggcggggcg catcggcatc
     5401 ctgccgggta accgttacca gcgtattgac tgatccggcg ctgaatcgta accgcccttg
     5461 ccaatccgtc cggcgcccac ttatcatgcc cggcacctga caggcagacc ccatgagcaa
     5521 aaaactgatc atcgccgaga aaccctccgt cgccagcgac atcgccaagg cgctcggtgg
     5581 tttcaccaag cacgacgaat acttcgaaaa cgacgaatac gtcgtctcgt cggccgtcgg
     5641 ccatttgctc gaactggcct gtccggaaga attcgaggtc aagcgcggca agtggtcctt
     5701 cgcccacctg ccggtgatcc cgccgcattt cgcgctgaac ccgatcgaga agagcgcacc
     5761 gcgcctgcgc gtgctcaaca agctgatcaa gcgcaaggat gtgaccggtc tgatcaacgc
     5821 ctgtgacgcg gggcgcgaag gcgagctgat cttcaattac atcgtccagc acgccaaggc
     5881 cggcaagccg gtgcagcggc tgtggctgca gtcgatgacg ccgcaggcga tccgcgacgg
     5941 cttcgcgcgg ctccgcccgg gcagcgaaat gcagggcctg gccgacgccg ccgtctgccg
     6001 ttccgaatcc gactggctgg tcggcatcaa cggcacccgg gcgatgaccg ccttcaactc
     6061 gaagaccggt ggcttccacc tgaccacggt cggccgcgtg cagacgccga cgctggcgat
     6121 cgtcgtcgag cgcgagcgca agatccgcga cttcgtgccg cgcgattact gggaagtcga
     6181 ggccgaattc gccgccaagg ctggcggcta caccggcaag tggttcgacg agggtttcaa
     6241 gggcaagcag gacgacgagc acgcccgtgc cgaccgactc tgggaagtcg cccgcgccga
     6301 agcgatcaag gcggcgacct cgggcaagcc cggcatcgtc agcgaggaag ccaagccgga
     6361 aacccgcctg tcgccgctgc tgttcgacct gaccagcctg cagcgcgagg ccaacagccg
     6421 tttcggtttc tcggccaaga ccacgctcgg cctggcccag gcgctctacg aaaagcacaa
     6481 ggtgctgacc tacccgcgga ccgactcgcg ctgcctgccc gaggactacc tggccacggt
     6541 caaggaaacg atgcgcgtgc tcaccggcga aggcgccggc aagggtcacg atgaagtgct
     6601 gctcgcccgt tacgcgccgt tcgcgcacca gatccttgca cgcaactggg tgctgccgaa
     6661 caagcgcatc ttcaacaacg cgaaggtgag cgatcacttc gccatcatcc ccacgccgca
     6721 ggcgccgaaa aacctcaacg aagccgagca gaagctctac gacttcgtcg tccgccgctt
     6781 cctgtcagtg ttcttcccgg ccgccgaata cctggtcacc acccgcatca cccgggtcga
     6841 aggccacccg ttcaagaccg agggcaaggt cctggtcaac ccgggctggc tcgccgtcca
     6901 tggcaaggaa ggccagactg gtgacgaagg caacctggtc g
//
LOCUS       DAOSDX010000025         6831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4057_length_6831_cov_3.294569, whole genome shotgun sequence.
ACCESSION   DAOSDX010000025 DAOSDX010000000
VERSION     DAOSDX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4057_length_6831_cov_3.294569"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1567)
                     /gene="pap"
                     /locus_tag="PLN02_01015"
     CDS             complement(65..1567)
                     /gene="pap"
                     /locus_tag="PLN02_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HOI50931.1"
                     /translation="MFESAELGHKIDKEVFKQEVPKLRAELLDVQYDVLQKKEFPVVI
                     LISGVDGSGKGETINLLYSWMDPRHISTLAFSAPSDEEAARPYMWRYWRALPAKGKVG
                     IFAGSWYSQPITDRITGDMRRAELDERLDDINRFEAMLVNEGALVLKFWFHLSKDAQK
                     KRLKALEKDPRTAWRVTPESYDRLKTYDKLQEVAGHVLRVTNTAKAPWIIVEGTDDAY
                     RSLTVGRIVLDAMRRRLQQPLQQVPVAPPIVHPIDQKNVLSTLDLTQKLGKKDYESQL
                     AKYQSRLSELVREPRFVGKHSLVLVFEGSDAAGKGGSIRRVGAAMDARQYQIVPIAAP
                     TEEERAQPYLWRFWRHLPRIGRAVIFDRSWYGRVLVERVEGFCSEADWLRAYAEINDF
                     EHQLVASGTIVVKFWLQISAEEQLRRFKERQDTEFKRFKITDEDWRNREKWDAYVAAV
                     CDMVDRTSTGLAPWTLVEANDKNFARVKVLKTVCERIETALKGEGKSETR"
     gene            complement(1571..2308)
                     /locus_tag="PLN02_01020"
     CDS             complement(1571..2308)
                     /locus_tag="PLN02_01020"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOI50932.1"
                     /translation="MNLPRFYCREALSPGAHVDLPEPVARHAVRVLRLPPGAPMVLFD
                     GRGGEYLAHIQRIERDRVVAELASWTEVERESPLQVTLVQALQAGDKMDFTIQKAVEL
                     GVSAIVPVDSRRSVLKLAGERAARRVAHWQGVAASACEQCGRNQVPIVAPLERLDQWL
                     ARPADGALRLMLAPDAETTLAGLAPAKHVQLLIGAEGGLDPQEVIAARQAGFQAVRLG
                     PRVLRTETAGLAALAALQALWGDFREG"
     gene            2489..4969
                     /locus_tag="PLN02_01025"
     CDS             2489..4969
                     /locus_tag="PLN02_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI50933.1"
                     /translation="MNIDTPTASAEDSRLRLAYLQAVVSSLPQGISVFDEHLQLRVWN
                     QGFIDLLALPPDLVKEGAHFSDLVRIPAQRGEYGPGDAEEHVRRITEQASLFNPQRLE
                     RTRPDGRTRLVDSEPLLINGKLAGFITTYTDITERKQAEEELRHQHALLETVVEAIPS
                     AVTMFDKNLNLLLHNREFQRLLELPDSLFAEQPVPIERIYRFNAERGEYGPGEPESIV
                     RALMARARHPVPHHFERTRPNGTTIDVRGVPLADGSFVTIYADVSERLASAEREKLAQ
                     VVFDHTPAGIAVTDEAHRLLSVNPAVSAMTGLTPDALLQTSVFDLIRADDNWSREQLH
                     ASLGLHGAWRGEVLVRRGEQGSFPASLRINRVEENTGNLPCHYIWIFADITERKEAEA
                     RMRHIAQHDSLTGLPNRLSLLMRLGQLLPEARRHGWKVGMMFIDLDRFKIINDTLGHQ
                     VGDEMLREVACRLSSVVRETDFVARLGGDEFVVILPGINSPADAALVASKIVNALATP
                     IEAEGHELHTSPSIGISLFPDDGPDGDAILKNADTAMYHAKSAGRNNYQFFAADMNQQ
                     ATERLDIERKLRHAIGRNELALVYQPQFGADNRATGVEALLRWHHPVDGMISPDRFIP
                     IAEETGMIVEIGDWVMLTACRDMKHWIDSGLVPLRVAVNVSARQLRRRDFCETVAGAL
                     AESGLPAELLELEITESSVMENPQEAIHILERLGRMGVSLAIDDFGTGYSSLAYLKLF
                     PIDHLKIDRSFVRDIEHDLNDRAIAFGTIALAHSLGLNVIAEGVETEDQLELLRSNGC
                     DEVQGYLFSKPLSSAAAFAFLHAMDRQA"
     gene            5003..6016
                     /locus_tag="PLN02_01030"
     CDS             5003..6016
                     /locus_tag="PLN02_01030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOI50934.1"
                     /translation="MAQRTLSRYLTEEHRNKGLIPANLKSLIEVVARACQAISIAIGK
                     GGLGGVLGEAGSDNVQGEAQKKLDVLSNEILLEANEWGGHLAAMASEEMDLPHLVPHR
                     FPKGEYLLTFDPLDGSSNIDVNVSIGTIFSVLKCPEGADLSSPEAAQAAFLQPGTAQV
                     AAGYAVYGPTTLLVLSVGDGVVVFTLDREQGQFVLTQEKVRIPEDTREFAINMSNRRF
                     WEAPVQRYVEEMTAGREGPLGKDYNMRWVASMVADVHRIMTRGGIFMYPLDEKVRSKG
                     GKLRLMYEANPMAFLVEQAGGAATTGRQRILDITPDGLHQRVPVILGSKNEVDRVTAY
                     HAA"
     gene            6218..>6831
                     /locus_tag="PLN02_01035"
     CDS             6218..>6831
                     /locus_tag="PLN02_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI50935.1"
                     /translation="MLLSACGKKEEAAPQTAAPAAPAIVTGGETIRWRLASSFPKALD
                     TIYGAADTFSAKVKELSGGKFEISVHPGGELVPPFGVLDAVQQGTVECAHTAPYYFFG
                     KDESFAMDCTIPFGLNSRQTTAWMFQGNGLKLLRELYAQYNIVNFPMGNTGAQMGGWY
                     RKEIKSLADINGLKMRIGGFGGRILAGIGGVPQNIPAGEIYQSL"
BASE COUNT         1240 a         2438 c         2050 g         1103 t
ORIGIN      
        1 gcgttgatgt agggcgtgac ggcccggaac caggagacga aattctcgtc gtaggcggtg
       61 tcgctcatct tgtttcggat tttccttcgc ccttgagggc ggtttcgata cgctcgcaga
      121 cggtcttcag gaccttgacg cgggcaaaat tcttgtcgtt ggcttcgacc agcgtccacg
      181 gcgccagccc ggtcgaggtg cgatcgacca tgtcgcagac cgccgccacg taggcgtccc
      241 atttttcgcg gttgcgccag tcctcgtcgg tgatcttgaa gcgcttgaac tcggtgtcct
      301 ggcgctcctt gaaccggcgc aactgctcct cggcggaaat ctgcagccag aacttgacga
      361 cgatcgttcc cgaagccacc agctggtgct cgaaatcgtt gatctcggcg taggcgcgca
      421 gccagtcggc ctcgctgcag aagccctcga cccgctcgac gaggacgcgg ccgtaccatg
      481 agcggtcgaa gatgaccgcc cgaccgatgc gcggcagatg ccgccagaaa cgccagaggt
      541 agggctgggc gcgctcttcc tcggtcggcg cggcaatcgg cacgatctgg tactgacggg
      601 cgtccatcgc cgcgccgacg cggcggatgc tgcccccctt gccggccgcg tccgagcctt
      661 cgaaaaccag caccagcgaa tgcttgccga cgaagcgcgg ttcgcgcacc aactcggaca
      721 ggcgcgactg gtatttggcg agctggctct cgtaatcctt cttgcccagc ttctgcgtca
      781 gatcaagcgt ggacagcacg ttcttctgat cgatcggatg gacgatcggc ggcgccaccg
      841 gcacctgctg cagcggctgc tgcaggcggc ggcgcatggc gtcgaggacg atgcggccga
      901 cggtcagcga acggtaggcg tcgtcggtgc cctcgacgat gatccacggc gccttggcgg
      961 tattggtcac gcgcagcacg tggccggcga cttcctgcaa cttgtcgtag gtcttcagac
     1021 ggtcgtagct ttccggcgtc acccgccagg cggtgcgcgg gtccttttcc agcgccttca
     1081 ggcgcttctt ctgggcgtcc ttggacaggt gaaaccagaa cttgaggacc agcgcgcctt
     1141 cgttgaccag catcgcctcg aaacggttga tatcgtcgag tcgctcgtcg agttcggcgc
     1201 gccgcatgtc gccggtgatg cggtcggtga tcggctgcga ataccaggag ccggcgaaga
     1261 tgccgacctt gcccttggcc ggcagcgcgc gccagtagcg ccacatgtag gggcgcgccg
     1321 cttcctcgtc gctcggcgca gaaaaggcca gcgtcgagat gtggcgcggg tccatccacg
     1381 agtagagcag gttgatggtt tcacccttgc cgctgccgtc caccccggaa atcaggatga
     1441 cgaccgggaa ctccttcttc tgcagcacgt cgtactggac gtcgagcaac tccgcgcgca
     1501 gcttcggcac ttcctgcttg aaaacttcct tgtcgatctt gtgaccgagt tccgccgatt
     1561 cgaacatcgc ctacccctcc ctgaaatccc cccaaagggc ctgcagcgca gcgagcgccg
     1621 ccaaccctgc cgtttccgtg cgcaacaccc gtggccccag gcgtaccgcc tggaaacccg
     1681 cctggcgggc ggcgatcact tcctgcggat cgagcccccc ttcggcaccg atcagcaact
     1741 gcacgtgttt cgccggcgcc aatccggcca gcgtcgtttc ggcatcaggc gccagcatca
     1801 ggcgcaaggc cccgtcggcc ggccgcgcca gccattgatc gaggcgctcc agcggcgcga
     1861 cgatcggcac ctggttgcgc ccgcactgct cgcaagccga agcagccacg ccctgccagt
     1921 gagcgacgcg cctggcggcg cgctcgccgg ccagtttcag cacgctgcgc cggctgtcca
     1981 ccgggacgat ggcgctgacg cccaactcga ccgccttctg gatcgtgaaa tccatcttgt
     2041 cgccggcctg cagcgcctgc accaaggtca cctgcaacgg cgattcgcgc tcgacctctg
     2101 tccatgacgc cagctcggcc accacccggt cgcgctcgat gcgctggata tgggccagat
     2161 attcgccgcc gcggccatcg aacagtacca tcggcgcccc cggcggcaag cgcaggacgc
     2221 gcacggcgtg gcgggccacc ggctcgggta gatcgacgtg ggcgcccggc gacagggcct
     2281 cccggcagta aaaacggggc agattcatcg ccgctattat gcggtgaaac cgcgcgacga
     2341 agggagcgtc atgcttgccg gcgaagcatc gcccggcgaa ttccgacgcg ccaccaccca
     2401 acgaccaccg ccatcgggta aatgcaggcg cgacgggggc aaggcatggg gtatcatccc
     2461 gcaatactgt gcagcttccg cccacgcctt gaacatcgat accccgaccg cttccgccga
     2521 agactcacgg ctgcgcctcg cctacctgca ggcggtggtt tccagcctgc cgcagggcat
     2581 cagcgtcttc gacgagcacc tgcagttgcg cgtctggaac cagggtttca tcgacctgct
     2641 cgccttgccg ccggatctgg tcaaggaagg ggcgcacttc tccgacctcg tccgcatccc
     2701 cgcccagcgc ggtgaatacg gccccggcga cgccgaggag catgtccggc ggatcaccga
     2761 gcaggccagc ctgttcaacc cgcaacgttt ggaacggacg cgcccggacg gccgcacccg
     2821 cctggtcgac agcgaaccac tgctgatcaa cggcaaactg gccggcttca tcaccaccta
     2881 taccgacatc accgaacgca agcaggccga ggaagaacta cgtcaccagc acgcgctgct
     2941 ggaaaccgtc gtcgaggcaa ttcccagcgc cgtcacgatg ttcgacaaga acctcaacct
     3001 gctgctgcac aaccgggagt tccagcgcct gctcgaactg ccggacagcc tgttcgccga
     3061 acagccggtg ccgatcgaac gcatctaccg cttcaacgcc gagcgcggcg aatacggccc
     3121 cggcgaaccg gaatcgatcg tccgcgcgct gatggcacgc gcccgccacc cggtgccaca
     3181 ccacttcgaa cgcacccggc caaacggcac aaccatcgac gtgcgcggcg tcccgctagc
     3241 cgatggcagc ttcgtcacca tctatgccga cgtcagcgaa cgactcgcct ccgccgaacg
     3301 ggaaaagctg gcgcaggtcg tgttcgacca cacaccggcc ggcatcgccg ttaccgacga
     3361 ggcgcaccgc ctgctgtcgg tgaacccggc agtctcggcg atgaccgggc tgaccccgga
     3421 cgccttgctc caaaccagcg tcttcgacct gatccgcgcc gacgacaact ggtcgcgcga
     3481 gcaattgcac gccagcctcg gcctgcatgg cgcctggcgc ggcgaagtcc tggtgcggcg
     3541 cggcgagcag ggcagtttcc cggccagcct gcgcatcaac cgggtcgagg aaaacaccgg
     3601 caacctgccc tgccactaca tctggatctt cgccgacatc accgagcgca aggaagccga
     3661 ggcgcgcatg cgccacatcg cccagcacga ttcgctgacc ggcctgccca accgcctgtc
     3721 gctgctcatg cgcctcggcc agttgctgcc ggaagcgcgc cgtcacggct ggaaggtcgg
     3781 catgatgttc atcgacctcg accgcttcaa gatcatcaac gacacgctcg gccaccaggt
     3841 cggcgacgag atgctgcgcg aagtcgcctg ccgcctgtcc agcgtcgtcc gcgagaccga
     3901 ttttgtcgcc cgcctgggcg gcgacgagtt cgtcgtcatc ctgcccggca tcaattcgcc
     3961 ggccgacgcg gcgctggtcg ccagcaagat cgtcaatgcg ctggcgacgc cgatcgaagc
     4021 cgaaggtcac gaactgcaca ccagcccgtc gatcggcatc agtctcttcc cggatgacgg
     4081 cccggacggc gacgccatcc tgaagaacgc cgacaccgcg atgtatcacg ccaaatcggc
     4141 tggtcgcaac aactaccagt tcttcgccgc cgacatgaac cagcaggcga ccgagcgcct
     4201 cgacatcgaa cgcaagctgc gtcacgccat cggccgcaac gaactggccc tggtctacca
     4261 gccgcagttc ggcgccgaca accgggcgac cggcgtcgaa gcgctgctgc gctggcacca
     4321 tccggtcgac ggcatgatct cgcccgaccg cttcatcccg atcgccgagg aaaccggcat
     4381 gatcgtcgag atcggcgact gggtcatgct caccgcctgc cgcgacatga aacactggat
     4441 cgacagcggc ctcgtcccct tgcgcgttgc cgtcaacgtc tcggcccgcc agttgcgccg
     4501 gcgcgatttc tgcgagacgg tcgccggcgc gctcgccgaa tccggcctgc cggccgaact
     4561 gctggaactg gaaatcaccg aaagctcggt gatggaaaac ccgcaggaag ccatccacat
     4621 cctcgaacgc ctgggccgca tgggcgtcag cctggcgatc gacgatttcg gcaccggcta
     4681 ttcctcgctc gcctacctca agctgttccc gatcgaccac ctgaagatcg accgctcctt
     4741 cgtccgcgac atcgagcacg acctcaacga ccgggcgatc gccttcggca ccatcgccct
     4801 cgcccattcg ctcggcctca acgtcatcgc cgaaggcgtc gagaccgaag accagctcga
     4861 actgctgcgc agcaacggct gcgacgaggt gcagggctat ctgttcagca agccgctgtc
     4921 gagcgccgcc gccttcgcct tcctgcacgc gatggaccgc caggcgtaaa atccgggttt
     4981 ttcctttttc ccggaatttc ccatggccca gcgcaccctt tcccgctacc tgaccgaaga
     5041 acaccgtaac aagggcctca tcccggccaa cctcaagtcc ttgatcgaag tcgtcgcccg
     5101 cgcctgccag gccatttcga tcgccatcgg caagggcggc ctcggtggcg tactcggcga
     5161 agccggcagc gacaacgtgc agggcgaggc gcagaagaaa ctcgacgtgc tgtccaacga
     5221 aatcctgctc gaagccaacg agtggggcgg ccacctcgcc gccatggcct ccgaggaaat
     5281 ggacctgccg catctggtac cgcaccgctt cccgaagggc gaatacctgc tcactttcga
     5341 cccgctcgac ggctcgtcga acatcgacgt caatgtctcg atcggcacca tcttctccgt
     5401 cctcaagtgc ccggaaggcg ccgacctgtc ttcgccggaa gccgcccagg ccgccttcct
     5461 gcaacccggc accgcgcaag tcgctgccgg ctacgccgtc tatggcccga ccacgctgct
     5521 ggtgctgagc gtcggcgacg gcgtcgtcgt gttcaccctc gaccgcgaac aggggcagtt
     5581 cgtgctgacc caggaaaaag tacgcatccc ggaagacacc agggagttcg ccatcaacat
     5641 gtcgaatcgc cgcttctggg aagcgccggt gcagcgctac gtcgaagaga tgaccgccgg
     5701 ccgcgaaggc ccgctgggca aggattacaa catgcgctgg gtggcctcga tggtggccga
     5761 cgtgcatcgc atcatgaccc gcggcggcat cttcatgtac ccgctcgacg aaaaggtccg
     5821 cagcaagggc ggcaagctgc gcctgatgta cgaagccaac ccgatggcct tcctcgtcga
     5881 gcaggccggc ggcgccgcca ccaccggccg ccagcgcatc ctcgacatca ccccggacgg
     5941 cctgcaccag cgcgtgccgg tcatcctcgg ctcgaagaac gaagtcgacc gcgtcaccgc
     6001 ctaccacgcg gcgtgaatcg ccagacggcg gccgattagg gaatctccta attcggcatt
     6061 gcggcgaatc ggcagaccat gacggctatc gaccccgaat agatcgatag tgcatccatc
     6121 cgccccgctt cggcggggca ccaaaacgag gagaccattc atggaaagac gcaagtttct
     6181 gaccaccgct tcgctgggcg ccgccgccgg cgccggcatg ttgctgagcg cctgcggcaa
     6241 gaaggaagaa gccgccccgc agaccgccgc cccggccgcc cccgccatcg tcaccggcgg
     6301 cgagaccatc cgctggcgcc tcgcttccag cttcccgaaa gcgctcgaca ccatctacgg
     6361 cgcggccgac accttttcgg ccaaggtcaa ggaactgtcc ggcggcaagt tcgaaatctc
     6421 cgtccacccc ggcggcgaac tggtcccgcc gttcggcgtg ctcgatgcgg tccagcaggg
     6481 caccgtcgaa tgcgcgcaca ccgcgccgta ttactttttc ggcaaggacg agagcttcgc
     6541 gatggactgc accatcccgt tcggcctcaa ctcccgccag accaccgcct ggatgttcca
     6601 gggcaacggc ttgaagctgc tgcgcgaact ctacgcccag tacaacatcg tcaatttccc
     6661 gatgggcaac accggcgccc agatgggcgg ctggtatcgc aaggaaatca agtcgctggc
     6721 cgacatcaat ggcctgaaga tgcgcatcgg cggcttcggc ggccgcatcc tggccggcat
     6781 cggcggcgtg ccgcagaaca tcccggccgg cgaaatctac cagtcgctgg a
//
LOCUS       DAOSDX010000026         6803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4081_length_6803_cov_3.268524, whole genome shotgun sequence.
ACCESSION   DAOSDX010000026 DAOSDX010000000
VERSION     DAOSDX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4081_length_6803_cov_3.268524"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLN02_01040"
     CDS             <1..204
                     /locus_tag="PLN02_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI50936.1"
                     /translation="KHFGGALVWDVNATLTIPTGIANQEAYATVSVNNVLDRKAALYD
                     NGNSLGVLYEYGRQFWLELGYRF"
     gene            221..778
                     /locus_tag="PLN02_01045"
     CDS             221..778
                     /locus_tag="PLN02_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOI50937.1"
                     /translation="MKRRDLLLGGLAVATFAHADVGGEAARVADPAPLDNELDKYPRC
                     VVCNMDRRQYHYARHLLHYGDGHAEGTCSINCAAECMLRERRRGFTAIYAPDYGVAGE
                     PKPLIEVATATYLIGSDLRGVMSPVSKYAFSERARAEAVQAVAGGRLGTFAEALAAAL
                     EDYARSLNRRYDGDREKLRRQGKLS"
     gene            complement(795..1493)
                     /locus_tag="PLN02_01050"
     CDS             complement(795..1493)
                     /locus_tag="PLN02_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOI50938.1"
                     /translation="MIDFSPAAGELPLFLAFMTGLLGSGHCLGMCGGLVSAFFIKLGA
                     RGPAPYLAYHGARIGIYAVVGVIAAGLGQVLVSTGWIGLWQGVLQIVAGVVVILLGLD
                     LLGVSPWRNSLGFAPVAWLRRQFTLAVQKGPVRGALIGGAINGLMPCSMTMAMAVKAT
                     TAASLGGGALLMLAFGAGTLPAMLTASFLFGRLGPRLRGWLLKGAALFVIALGAATLW
                     QGLRYYLVMYRLIV"
     gene            complement(1778..4204)
                     /locus_tag="PLN02_01055"
     CDS             complement(1778..4204)
                     /locus_tag="PLN02_01055"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOI50939.1"
                     /translation="MKHFRVAHRLRRRLRIVSPVLARHAERCYLLEILLRKQPAVREA
                     SVVVDIGSLTVRFDPDALPEARLLATLDAILGHLQAAPPHAPVAPPPADAPLSECSLS
                     VEGMTCASCALLIEMKLRRDPRVREANVNFAAASVNVRGAIDRDGVAEVIRRLGYAAQ
                     PMDTLAQRRQVIAREQARIAEARRRSVRSALLSAPVVLSGMLMHRSPALRLVEFALSS
                     AVLFGSGGEIFRKAWQLARQGEANMDTLIAIGAGAAWGYSLPGLFGRRHHVYFESAAA
                     ICAFVLFGRYLEERAKGKAGEAIRQLIELQPETALRIAADGAESEVPIDDVRLGDRLR
                     VRAGARVPSDGIVVDGASSVDEAMLSGESLPVAKAPGDRVVGGCINGNGSFVLRVTAV
                     GADTVLSGIVRLVDQAQGSKLPVQKLADRISARFVPAVAGVAGLTFGGWALAGAPAAT
                     ALSHAVAVLLIACPCALGLATPTAIMVGTGQAARRGIYIRHGEALETAAQLTTLVFDK
                     TGTITAGRPTVSDAVLAAGQDEGRLAALVGAAESGSEHFLARALGDWCHGRQAGALNA
                     ESFAALPGRGIVARVDGQQLVIGNADLLREQGVDPAPWQETAERFAAQGKTPVYVALD
                     GVPAAIFAIADQARPGAREAIALLHRLGLQTVMATGDVDAVARHVAAEVGIERVLARL
                     TPADKLALVRELQAAGGKVGMVGDGINDAPALTAADVGFAIGGGAGIAVEAAAVTLVG
                     GDIARVAGGIDLSRRTMAIIRQNLFWALGYNVVAIPFAAAGRLTPMIAAAAMAMSSLS
                     VVGNSLRLQR"
     gene            complement(4201..4767)
                     /locus_tag="PLN02_01060"
     CDS             complement(4201..4767)
                     /locus_tag="PLN02_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50940.1"
                     /translation="MANARQLAERIVVRERGSGYLCLALPGELCAAPLAAGIETGMAG
                     LAGVRRAHVDAAGQSLTLGFDAALLSTASVARHLFSLLERLPEEAAGAAPALGLQPAL
                     DRLKTILIPPGEAPPGSLRARVQPVLESALTEKAMTNFFNDIVAFYLIKTHWDLITKR
                     WLQNPLAHSNAWLTVFYLVFLLVRYRKT"
     gene            complement(4778..5155)
                     /locus_tag="PLN02_01065"
     CDS             complement(4778..5155)
                     /locus_tag="PLN02_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50941.1"
                     /translation="MIKESMMKQVPQMLCDAGQALAKPAGSGALMAAGGVAAGRSLLG
                     RAALPNPLALLALGAVGGIAAGFLLFKYQKEITEGLGKLGGMGKDFALNRKENLNDLM
                     AEAEGKLSGVQPPAAATGDAERT"
     gene            complement(5152..5349)
                     /locus_tag="PLN02_01070"
     CDS             complement(5152..5349)
                     /locus_tag="PLN02_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HOI50942.1"
                     /translation="MSRDETRCTLCGLDVGVRPFTLKVGDQAYRFCCEGCRGIYEMLH
                     DIQAPPGGADEISANPGKDEK"
     gene            complement(5346..5834)
                     /locus_tag="PLN02_01075"
     CDS             complement(5346..5834)
                     /locus_tag="PLN02_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOI50943.1"
                     /translation="MRPLILSFLLLLAACGGGEQPKVNVGLAAPAFTTQRADGTPVNF
                     PADLAGRPVVLRFWADWCRYCEGEMKAIETVYGRLQPRGLVVLAMNAGQDKATVDAFM
                     GKIGVSYPALLDGDSAIARRYGVVGLPTTFFIDGQGMVRGKIVGEADAATFEREAAAL
                     LQ"
     gene            complement(5891..>6803)
                     /locus_tag="PLN02_01080"
     CDS             complement(5891..>6803)
                     /locus_tag="PLN02_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI50944.1"
                     /translation="LLAQTDIDRLAGLPGVAEVQGRLWGYYYDSVVKANYTLMARPDA
                     EVAEGTLSIGNAVARERGLVPGNTISFRSYSGKLFSFKVATLLPEDSELLSGDLVLLA
                     AGDFRRFFEYPAGQYTDAVLRSTGAQSGDALAAAIRAAQPELRPIPRAEILAIYAQLF
                     GWRDGIGLAVLAGVILAFVILAWEKAAGLPAEERREIGILKAIGWEGSDVIRMKLWEG
                     LLIALAAFLIGFVAAWLHVFRYDAALFAPALRGWSVLYPSFRLSPVVDLRQLATLFLF
                     TVLPYALTVLLPVWRAARAEPDAVMRA"
BASE COUNT         1119 a         2387 c         2367 g          930 t
ORIGIN      
        1 aaacatttcg gcggcgcgct ggtctgggac gtcaatgcca cgctgaccat cccgactggc
       61 atcgccaacc aggaagccta tgcaacggtc tcggtcaaca acgtgctcga ccgcaaggcc
      121 gcgctctacg acaacggcaa cagcctcggc gtactctacg agtacgggcg ccagttctgg
      181 ctcgagctgg gttaccgctt ctgatgtacg ggagatttcc atgaaacgaa gagacctgtt
      241 gctcggcggc ctggccgtcg ccaccttcgc ccacgccgat gtcggcggcg aagctgcccg
      301 cgtcgccgac cccgccccgc tcgacaacga actcgataag tacccacgct gcgtcgtctg
      361 caacatggac cgccgccagt accactatgc ccgtcacctg ctgcattacg gcgatgggca
      421 tgccgagggg acctgttcga tcaattgcgc ggcggagtgc atgctgcgcg agcggcggcg
      481 cggcttcacg gcgatctacg cgccggatta cggcgtcgcc ggcgagccga agccgttgat
      541 cgaggtggcg acggcgacct acctgattgg ctccgacctg cgcggggtga tgagcccggt
      601 cagcaagtac gctttttccg aacgcgcccg cgccgaggcg gtacaggcgg ttgccggcgg
      661 ccgtctcggc acctttgccg aggcgctggc ggcggcgctg gaggactatg cgcgcagcct
      721 gaaccggcgt tacgacggcg accgggaaaa gctcaggcgc cagggcaagc tctcgtaagg
      781 ccggcgggcc gggctcagac gatcagccgg tacatcacca agtagtagcg cagcccctgc
      841 cacagcgtcg cggcgcccag cgcgatgacg aagagtgccg cgcccttgag cagccagccg
      901 cgcaggcgcg ggccgaggcg gccgaaaagg aacgaggcgg tgagcatcgc cggcagggtg
      961 ccggcgccga aagcgagcat gagcagtgcg ccgccgccga gcgaggcggc ggtggtggcc
     1021 ttgaccgcca tcgccatggt catcgagcag ggcatcaggc cgttgatcgc gccaccgatc
     1081 agcgcgccgc gcaccgggcc tttctgcacc gccagcgtga actgccggcg cagccaggcg
     1141 accggcgcga agccgaggct gttgcgccat ggcgaaacgc cgagcaggtc gaggccgagc
     1201 aggatcacga cgaccccggc gacgatctgc aacacgccct gccagaggcc gatccagccg
     1261 gtggacacga gcacctggcc aagcccggcg gcgatcacgc cgaccacggc gtaaatgccg
     1321 atgcgcgcgc cgtggtaggc gagataagga gcggggccgc gcgcgccgag cttgatgaag
     1381 aaagcggaca ccagcccgcc gcacatgccc aggcagtggc cgctgccaag cagcccggtc
     1441 atgaaggcga ggaacagcgg cagttcgccg gccgccggag aaaaatcgat caagcttgct
     1501 acctcggttg gacgcggccg ctggcgcgtt gggacgggag ggcaggcgcg cagcgacgac
     1561 gcggcaggct catgccggca aggcggcgcg acaacgcgcc ggcgaaaatt tcggcagacg
     1621 gcgagcttcg gcgcagccac aacgccgggc gcccgtcccg gcctgcctgc ggccgtgtag
     1681 cgccatcagc caaagcgtga ctcgcggcgc aggcttggag gtcggcatgc acgcggcggc
     1741 acgaggagag atcagcggtc aactgaggtt tcaaggttca ccgttgcagg cgcagcgagt
     1801 tgccgaccac cgacagcgaa ctcatcgcca tcgcggcggc ggcgatcatc ggcgtcagcc
     1861 ggccggcggc ggcgaaggga atggcgacga cgttgtagcc gagcgcccag aacaggttct
     1921 ggcggatgat cgccatcgtc cgccgcgaca ggtcgatgcc gcccgcaacg cgggcgatgt
     1981 cgccgccgac cagcgtcacc gccgccgcct cgacggcgat gccggcgccg ccgccgatgg
     2041 cgaagccgac gtcggcggcg gtcagcgccg gggcgtcgtt gatgccgtcg ccgaccatgc
     2101 caaccttgcc gccggcggcc tgcagttcgc gcaccagcgc cagcttgtca gccggcgtca
     2161 ggcgggcgag cacgcgctcg atgccgactt cggcggcgac gtggcgggcg accgcgtcga
     2221 catcgccggt cgccatcacg gtttgcaggc cgagccggtg cagcagcgcg atcgcctcgc
     2281 gggcgcccgg acgggcttgg tcggcaatcg cgaagatcgc cgccggcacg ccgtcgagcg
     2341 ccacatacac cggcgtcttg ccttgcgccg cgaagcgctc ggcggtttcc tgccagggcg
     2401 ccggatcgac gccctgctcg cgcaggagat cggcgttgcc gatcaccagt tgctgcccat
     2461 ccacccgcgc gacgatgccg cgcccgggca gcgcggcgaa gctttcggcg ttcagcgcgc
     2521 cggcctgccg cccatggcac cagtcgccca gcgcgcgcgc caggaagtgt tcggaaccgc
     2581 tttcggcggc gccgaccagc gccgccagcc ggccctcgtc ctggccggcg gcgaggacgg
     2641 catcgctgac cgtcggccgg cccgcggtga tggtgccagt cttgtcgaac accagcgtcg
     2701 tcagttgcgc cgccgtttcc agcgcctcgc cgtggcggat gtagatgcca cggcgcgccg
     2761 cctggccggt gccgaccatg atcgccgtcg gcgtcgccag gccgagcgcg cagggacagg
     2821 caataagcag cacggcgacg gcatgcgaca gcgccgtcgc cgccggcgcg ccggccagcg
     2881 cccagccgcc gaaggtaagc ccggcgacgc cggccaccgc cggcacgaaa cgcgccgaaa
     2941 tgcggtcggc cagtttctgc accggcagct tgctgccctg cgcctgatcg accaggcgga
     3001 cgatgccgga gagcacggtg tcggcgccga ccgccgtgac gcgcagcacg aagctgccgt
     3061 tgccattgat gcagccgccg acgacgcggt cgccgggggc cttggcgacc ggcaaggatt
     3121 cgccgctgag catcgcttcg tcgacgctgc tcgcgccatc gacgacgatg ccgtcgctcg
     3181 gcacgcgggc gccggcgcgc acgcgcaggc gatcaccgag gcgcacgtcg tcgatcggaa
     3241 cttcgctttc cgcgccgtcg gccgcaatgc gcagcgcggt ttccggctgc agttcgatca
     3301 actggcggat cgcctcgccg gccttgccct tggcgcgctc ttccaggtag cggccgaaca
     3361 gcacgaaggc gcagatcgcc gccgccgatt cgaaatagac gtgatgacga cgcccgaaca
     3421 ggccgggcag gctgtagccc caggcggcgc cggcgccgat ggcgatcagc gtgtccatgt
     3481 tggcctcgcc ctggcgggcg agctgccagg ccttgcggaa gatctcgccg ccactgccga
     3541 agagcaccgc cgacgacagc gcgaactcga ccaggcgcag tgccggcgaa cggtgcatca
     3601 acatcccgga cagcaccacc ggcgcactga gcagcgccga gcgcacactg cgccggcgcg
     3661 cctcggcgat gcgcgcctgc tcgcgggcga tcacctggcg gcgctgcgcc agcgtgtcca
     3721 tcggctgtgc cgcgtagccg aggcggcgga tcacttcggc gacgccgtcg cggtcgatcg
     3781 cgccgcgcac gttgacgctg gcggcggcga aattgacgtt ggcctcgcgc acccgcgggt
     3841 cgcgccgcag cttcatctcg atcagcagcg cgcacgaggc gcaggtcatt ccttccaccg
     3901 acagcgagca ctcgctgagc ggcgcgtcgg ccggcggcgg tgccaccggc gcgtgcggcg
     3961 gcgctgcctg caggtggccg agaatggcgt cgagcgtcgc cagcaggcga gcctcgggca
     4021 gcgcgtcggg gtcgaaacgg acggtcagcg aaccgatatc gacgacgacg ctggcctcgc
     4081 gcaccgccgg ctgcttgcgc aacaggattt ccagcaggta gcagcgctcc gcgtgacgcg
     4141 ccagcaccgg cgaaacgatg cgcaggcgac ggcgcaggcg gtgggcaacg cggaaatgct
     4201 tcatgtcttg cggtagcgga ccagcaggaa gaccaggtag aagacggtca gccaggcgtt
     4261 gctgtgggcc agcggatttt gcagccagcg cttggtgatc aggtcccagt gcgtcttgat
     4321 caggtagaag gcgacgatgt cattgaagaa attggtcatc gccttttccg tcagcgcgct
     4381 ttccagcacc ggctgcacgc gggcgcgcag cgaaccgggc ggcgcctcgc cgggcgggat
     4441 caggatcgtc ttcaggcggt cgagcgccgg ctgcaggccg agcgccggcg ctgcgccggc
     4501 cgcctcttcc ggcaggcgtt cgagcaggga gaacaggtga cgggcgacgc tcgccgtcga
     4561 tagcagcgcc gcgtcgaaac ccagcgtcag cgactgtccg gccgcatcga catgggcacg
     4621 gcggacgccg gccagcccgg ccatgccggt ttcgatgccg gcggcgagcg gcgccgcgca
     4681 gagttcgccg ggcagcgcca ggcacaggta accgctgccg cgttcgcgga cgacgatgcg
     4741 ttccgccagt tgccgcgcgt ttgccatcgc cgcctcctca ggtccgttcc gcgtcgccgg
     4801 tcgcggcggc gggcggctgg acgccggaca gcttaccctc ggcctcggcc atcaggtcgt
     4861 tgagattttc cttgcggtta agcgcgaagt ccttgcccat gccgcccagt ttgcccagcc
     4921 cctcggtgat ttccttctgg tacttgaaaa gcaggaaacc ggcggcgatg ccgccgaccg
     4981 cgccgagcgc cagcagcgcc agcgggttgg gcagcgccgc ccggccgagc aggctgcgcc
     5041 cggccgccac cccgcccgcc gccatcagcg cgccactgcc ggccggcttg gccagcgcct
     5101 ggccggcgtc gcacagcatc tgcggcacct gtttcatcat gctttccttg atcatttttc
     5161 gtcctttccc ggattggctg aaatttcatc ggcaccgccc ggcggcgcct ggatgtcgtg
     5221 cagcatttca tagatgccgc gacatccttc acagcagaaa cgatacgcct gatcgccgac
     5281 cttgagcgtg aaaggccgga cgccgacatc caggccgcac aaggtgcagc gcgtctcgtc
     5341 ccggctcatt gcaggagcgc cgccgcttcg cgctcgaaag tcgcggcgtc ggcctcgccg
     5401 acgatcttgc cgcgcaccat gccctggccg tcgatgaaga aggtggtggg caggccgacc
     5461 acgccgtaac gcctggcgat cgccgaatcg ccgtcgagca gcgccggata gctgacgccg
     5521 atcttgccca tgaaggcatc gaccgtcgcc ttgtcctggc cggcattcat cgccagcacg
     5581 accaggccgc gcggctgcag gcggccgtag acggtttcga tcgccttcat ctcgccttcg
     5641 cagtagcggc accagtcggc ccagaaacgc agcaccaccg gccgcccggc gagatcggcg
     5701 ggaaaattca ccggcgtgcc gtcggcccgt tgcgtggtga aggcgggcgc cgccaggccg
     5761 acattgacct tgggctgctc gccgccaccg caggcggcga gcagcaggag gaaggagagg
     5821 atgaggggac gcactttcgc tccgggtcgt tgggcaatgt caggacgata agcttgacgc
     5881 ggcggagcaa tcaagcccgc atcacggcat cgggctcggc acgcgccgcc cgccacaccg
     5941 gcaacagcac ggtcagcgca tagggcagga cggtgaacag gaagagggtc gccagttgcc
     6001 gcagatcgac caccggtgac aggcggaagc tggggtaaag caccgaccag ccgcgcaggg
     6061 ccggcgcgaa caaggccgca tcgtagcgga agacatgcag ccaggcggcg acgaagccga
     6121 tcaggaaggc cgccagcgca atcagcaggc cttcccagag cttcatccgg atgacgtcgc
     6181 tgccctccca gccgatcgcc ttgaggatgc cgatttcccg cctttcctcg gcgggcaggc
     6241 cggccgcctt ttcccaggcg aggatgacga aggccaggat cacgccggcc agcacggcga
     6301 ggccgatgcc gtcgcgccag ccgaacagtt gggcgtagat ggcgaggatt tcggcgcgcg
     6361 ggatcggccg caactccggc tgcgccgccc ggatcgccgc cgccagcgcg tccccggact
     6421 gcgctccggt gctgcgcagc acggcatcgg tatactggcc ggccgggtat tcgaagaagc
     6481 ggcggaaatc gcccgccgcc agcaacacca ggtcgccgct cagcaattcg ctgtcctcgg
     6541 gcagcagcgt cgccaccttg aagctgaaca gtttgccgga atagctgcga aaggaaatgg
     6601 tgttgcccgg caccaggccg cgttcgcgcg ccaccgcgtt gccgatgctc agcgtaccct
     6661 cggcgacttc ggcgtccggg cgcgccatca gcgtgtagtt ggccttgacc acgctgtcgt
     6721 agtaatagcc ccacagccgc ccctgcacct cggccacccc gggcaggccg gccagacggt
     6781 cgatatcggt ctgcgccagc agg
//
LOCUS       DAOSDX010000027         6696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4157_length_6696_cov_3.239572, whole genome shotgun sequence.
ACCESSION   DAOSDX010000027 DAOSDX010000000
VERSION     DAOSDX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_6696_cov_3.239572"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..86
                     /locus_tag="PLN02_01085"
     CDS             <1..86
                     /locus_tag="PLN02_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50945.1"
                     /translation="RRGMVGGQEDMIEDTAITMARERGLAA"
     gene            219..1247
                     /locus_tag="PLN02_01090"
                     /pseudo
     CDS             219..1247
                     /locus_tag="PLN02_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            1269..3074
                     /locus_tag="PLN02_01095"
     CDS             1269..3074
                     /locus_tag="PLN02_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HOI50946.1"
                     /translation="MQRDSGLDPRHVLALFLAALAVAPLLLPEFYVTLLNYVGLSALV
                     ALGLVLLTGVGGLTSFGQAAFVGIGAYTTAWLTTATELPAWLAFAAGSPWMALLLGLV
                     LTATVAVLLGSVTLKLSGHYLPLGTMAWGISLFFLFGNMAFLGGYAGLSNLPAIEIFG
                     FALSDNRHFYYLVWAFVLAALFTTRNLLDSREGRAIRALKGGIVMAETMGVDTSRARM
                     VIFVIAALHACAAGWLYAHLQRFVNPTPFGLHIGIEYLFMAVVGGAGQVWGALVGAGV
                     ITVLKQWLQDVLPKLFGSAGHFEVIVFGLMMIVILHRARAGLWPLLARLVPVKTTPKV
                     VDAGAQPLLRKKQPPHGEIILEAKNVTRKFGGLVANNNMSLRVKAGEILALIGPNGAG
                     KSTMFNQLSGVDTPTSGDVLFRGQSVAGHDSREIAALGMSRTFQHVRLLPNMSVLENV
                     AIGAHRRGSKGVLSAAWRLDRAEEARLLREAAIQVERVGLGEFMHVEAGSLALGQQRI
                     LEIARALCSDPCLLLLDEPAAGLRLKEKQALAELLKRLRGEGMAVLIVEHDMDFVMNL
                     VDRVVVMEFGEKIAEGLPEEVQKDPAVLEAYLGGV"
     gene            3076..3837
                     /locus_tag="PLN02_01100"
     CDS             3076..3837
                     /locus_tag="PLN02_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI50947.1"
                     /translation="MSGNVLEVKGLSVAYGKVEAVSKLNLTVGEGKIVTVIGPNGAGK
                     TTTLSAIMGLLPSTGAVEFDGSVEHEPEVERMVVRGMTLVPEKRELFTTMSIEDNLLL
                     GAFQRNRMGHRDHLQTMDEVYALFPRLEERRLQPAGTMSGGERQMLAVGRALMAKPKL
                     LMLDEPSLGLAPLIVREIFRIIGELRRRGVSILLVEQNARAALQVADYAYVLENGAVK
                     MEGEAELLKNDPRVIESYLGLASKHQDRLSCESPT"
     gene            3859..4443
                     /locus_tag="PLN02_01105"
     CDS             3859..4443
                     /locus_tag="PLN02_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOI50948.1"
                     /translation="MEALHIIGEEHQSLAAVLHAIRFMLKEVAAGRLSPDLKLFQAMV
                     RYLDAYAEQRHHPKEDRLFNYLSLRSGEGKEALDKLAEQHLAAPQRIAALQHAVDDYV
                     ADPARFAEFAQAFEQYADFYRSHMQLEEEVVLPLVRRYLTAEDWAAVNAEFREEMAAN
                     DLGDASKADFKAFFSKLVACAPAPLGLGPRPWQD"
     gene            4556..5413
                     /locus_tag="PLN02_01110"
     CDS             4556..5413
                     /locus_tag="PLN02_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI50949.1"
                     /translation="MTLPTEAECSRLIRIQEGELDLQLHYNEIGAGAETVIMLHGSGP
                     GASGWANFNRNIEPLVDAGYRVILMDCPGWSKSDPVVCTGSRSELNARALKGLMDALR
                     IERAHLIGTSMGAHSVTAFALANPTRIGKLVLMGGGTGGPSQFVPMPTEGIKLLQALY
                     RDPSIENLKRMMAVFVYDASSLTEELFQARLDNMLQRREHLDNFVKSLAANPRQFTDY
                     GPRLSEISAPTLVIWGRDDRFVPMDIGLRLLWGMPNAELHIFNKCGHWAQWEHARKFN
                     AMVLDFLRD"
     gene            complement(5489..6649)
                     /locus_tag="PLN02_01115"
     CDS             complement(5489..6649)
                     /locus_tag="PLN02_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOI50950.1"
                     /translation="MPRSAARLADIAPFHVVELLTRARELEAAGRDIIHMEVGEPDFA
                     TPAPIVAAAVDALNSGKTLYTQALGLPELRQAISDFYRHRYGVAVPASRIAVTNGASG
                     ALNLAFACLADPGREWLLADPGYPCNRHILRTYEGRPVSIPVGPESNFQPTPAQVGAA
                     WNEHTAGLLVASPANPTGTLLTLPEIAALAEVCRARDGHFLVDEIYHGLTYECDAPTA
                     CAAGDDIWVINSFSKYFQMTGWRLGWMVILEAFVRDVEKLAQNLVLCPSTPAQYAALA
                     AFRPETIAILEERRAEFRRRRDFLAPALEAIGLSVVARPEGAFYLYCDCSAVAADSFA
                     LARKLLEDAGVAATPGLDFGANAPEKYIRFAYTTGVDRLAEAVERLRRHFGR"
BASE COUNT         1143 a         2025 c         2183 g         1345 t
ORIGIN      
        1 gccggcgcgg catggtcggc ggccaggaag acatgattga agacaccgcc atcaccatgg
       61 cacgagaacg gggactggcg gcttgatggc ttgcaccgcc tttcttgccg cttcgggcaa
      121 gtgtacgaag cggcaaggca cgaggggtat tgcccttttc aagttgaccg caaagggccg
      181 gattgccggc gctttgctgc tgtaacagga gtctcttgat ggatgtctca atctttgcgc
      241 tgctggcgca ggacgggatc accaacggcg cgatttacgc gcttctggcg ctggcgctgg
      301 tgctggtttt caccgtgacc cgaattatct tcattcccca aggggagttc ctcgcctggg
      361 gggcgctgac catggcgctg ctcgaagccg gccagaagcc tggcacggtc tggctgctac
      421 tgggggctgg cgtgctgacc ttcattgccg atgtcgtgca ggcgcgttcc ggtcgccgac
      481 cgctactccg ctcgggcctc tggaacctgg cctatccggc ggcggttgtt gcattggtct
      541 ggttgttgcc gctggctgaa ctgcccgctg tgctcaagtt cgcgctcacg ctggcgattg
      601 tcgtgccgat ggggccgatg ctctatcgca tcgtcttcca gccgattgcc gaagcctcgg
      661 tgctggttct actgatcgtt tcggtcgcgt tgcacttgct gatggtcggt ttcggcctcc
      721 tgatcttcgg gccggaaggt tcgcggacac ttcctttcag cgaagcgcag atcgaagtcg
      781 gcgcactgat gatcggcggt catacgctgg tcgtggtgtt cgctgcgctc gcgctgatcg
      841 tcggactatt tctctatttc aagcacagcc tttcgggaaa agccttgctg gcgaccgcga
      901 tcaaccgcaa cggggcgcgt ctgatgggta tttcaccggc gcttgccggg cgccagacct
      961 tctttgtcgc ggcgctgatc ggcgcactgt cgggcatgct ggtcgcgccg ctgactaccg
     1021 tctattacga ctccggtttc ctgatcggcc tcaagggctt tgtcgccgcg atcatcggcg
     1081 gccttgccag ctatcctctg gctgccgccg gcgccatcgt cgtcggcctg ctcgaatcct
     1141 tttcctcttt ctgggccagc gcctacaagg atgtgctggt attcacgctg atcatcccgg
     1201 tgctggtctg gctgtcctgg cgtacgcatc acatcgagga agaagatacc ggtcatctgc
     1261 cggccaccgt gcagcgcgac agcggtctcg atccccggca tgtacttgcg ctcttccttg
     1321 ccgcgcttgc cgtagcgcca ctgctgctgc ccgagttcta cgtgaccctg ctgaactatg
     1381 tcggcctgtc ggcgctggtg gccctcggcc tggtgctgct gaccggcgtc ggtggtctga
     1441 cttcgttcgg tcaggcagcc tttgtcggca tcggcgccta caccacagcc tggctgacga
     1501 cggcgaccga gttgccggcg tggctggctt ttgccgcagg atctccgtgg atggcgctgc
     1561 tgctcggcct tgtactgacg gcgacggttg ccgttctcct cggctcggtg acgctcaagc
     1621 tgtccggcca ctatctgccg ctcggcacca tggcctgggg tatcagcctg ttcttcctgt
     1681 tcggcaacat ggcattcctc ggtggctatg ccggcctgtc gaatttgccg gcgatcgaaa
     1741 tcttcggctt tgcattgagc gataaccgac atttctatta cctggtttgg gccttcgtcc
     1801 tggcggcctt gttcacgacc agaaacctgc tcgattcccg cgaaggccgc gccatccgcg
     1861 cgctcaaggg cggcatcgtg atggccgaga cgatgggggt cgatacctca cgggcgcgca
     1921 tggtgatctt cgtcattgcc gccttgcacg cttgtgcagc cggctggctg tacgctcatc
     1981 tgcagcgctt cgtgaatcca accccctttg gtctgcatat aggcatcgag tacttgttca
     2041 tggccgtcgt cggtggcgcc gggcaggtct ggggggcgtt ggtcggcgcc ggcgtgatta
     2101 ccgtgctcaa gcagtggctg caggatgtgc tgcccaaact tttcggttcg gctgggcatt
     2161 tcgaagtgat cgtcttcggc ttgatgatga tcgtcatcct gcatcgcgcc cgggccggcc
     2221 tgtggccgct ccttgctcgc ctggtaccgg tgaagacgac gccgaaggtc gtcgatgccg
     2281 gtgcgcagcc gctgctcagg aagaaacagc cgccgcacgg cgaaatcatc ctcgaagcga
     2341 agaacgtcac gcgcaagttc ggtggtttgg tcgccaacaa caacatgagt ctgcgcgtga
     2401 aggctggcga aatcctggcg ctgatcggcc ccaacggcgc gggcaagagc accatgttca
     2461 atcagctttc cggtgtcgac acgccgacct ccggcgacgt gctgttccgc ggccagtcgg
     2521 ttgccggcca cgattcgcgc gaaatcgccg cgctgggcat gagccgtacc ttccagcatg
     2581 tgcgtctgct gcccaatatg agcgtgctcg aaaacgtcgc catcggagcc catcggcgcg
     2641 gcagcaaggg cgtgctctcg gctgcctggc gcctggatcg agccgaggaa gcgcgtttgc
     2701 tgcgcgaagc ggcaatccag gtcgagcgtg tcggcctagg tgagttcatg catgtcgaag
     2761 ccggcagtct ggcgctgggc cagcagcgca ttctcgaaat cgctcgtgcc ttgtgttccg
     2821 atccctgcct gctgctgctc gatgagcctg ctgccggctt acgcctcaag gaaaaacagg
     2881 cgcttgccga attgctcaag cgtttgcgcg gcgaaggcat ggccgtactg atcgtcgagc
     2941 acgacatgga tttcgtgatg aacctggtcg accgcgtggt ggtgatggaa ttcggggaaa
     3001 agatcgccga aggcttgccg gaagaggttc agaaagatcc ggcggtgctt gaagcctatc
     3061 tgggaggggt ttgagatgag cgggaacgta ctggaggtca agggcctttc ggtggcctac
     3121 ggcaaggtcg aggcggtcag caagctcaac ctcacggtcg gcgaaggcaa gattgtcacc
     3181 gtgattggcc ccaatggcgc cggtaagacg acaacgctgt cggcgatcat gggcctgctg
     3241 ccgtcgaccg gtgcggtcga gttcgacggt tcggtcgagc acgagccgga agtcgagcgc
     3301 atggtcgtgc gcggcatgac gctcgttccg gaaaagcgtg aactgttcac cacgatgagc
     3361 atcgaggaca acctgctgct cggcgctttc cagcgcaatc gcatgggcca tcgcgatcac
     3421 ctgcagacga tggatgaggt gtacgcgctt ttccctcgcc ttgaggagcg ccgcctccaa
     3481 ccggccggca cgatgtcggg aggcgagcgc cagatgctgg cagtcggccg ggcgttgatg
     3541 gccaagccca agctgctgat gctcgacgag cccagtcttg gcctggcgcc actgatcgtg
     3601 cgggaaatct ttcgcatcat cggtgagttg cgtcgccgtg gggtctcgat tctgctggtc
     3661 gaacaaaacg cacgtgccgc actgcaggtc gccgattatg cctatgtgct ggagaacggg
     3721 gcggtcaaga tggaaggcga ggcggaattg ctgaagaacg acccacgggt gatcgagagc
     3781 tatctcggtc tcgccagcaa gcatcaggac aggttgtcct gcgaatcccc aacttgaacg
     3841 atcgaagaga gaacaatcat ggaagccctg catatcatcg gcgaggagca tcagtccctg
     3901 gcggcggttc tgcacgccat ccgcttcatg ctgaaggaag ttgccgccgg tcgtctgagt
     3961 cccgacctga aactctttca ggccatggtg cgttacctcg atgcctatgc cgagcagcgt
     4021 catcatccca aggaggacag gcttttcaac tacctgtcgc tgcgtagcgg agaggggaaa
     4081 gaggcgctcg acaaactggc ggagcagcat cttgccgcgc cccaacgcat tgccgcgcta
     4141 cagcatgccg tcgatgacta cgtggccgat ccggcgcgct ttgccgagtt tgcccaggcc
     4201 ttcgagcaat acgccgattt ctatcgcagc cacatgcaac tggaagaaga agttgttctg
     4261 ccgctcgtgc gccgttatct gacggcggaa gactgggctg cggtcaacgc cgaattcagg
     4321 gaagaaatgg ccgccaatga ccttggcgat gccagcaagg cggatttcaa agcgtttttt
     4381 tccaaacttg tcgcctgcgc cccggcgccg ctcggcttgg gaccgcgtcc ttggcaggat
     4441 tgaagatgcc gccaaacatc ccggcacccg ccttgctctt tcccgtttcc ctccccgctg
     4501 ggcggacggc tagcaactac cccgaaaaat tgatcgtaca gaaaggatcg tttccatgac
     4561 actcccgacc gaagccgaat gcagccgcct gatccgcatc caggaaggcg agctcgacct
     4621 gcaactccat tacaacgaga ttggcgccgg cgccgaaacc gtcatcatgc tgcacggatc
     4681 ggggccgggg gcgagcggct gggcgaattt caaccgcaat atcgagccct tggtcgatgc
     4741 cggctaccgc gtcatcttga tggactgtcc aggctggagc aagagcgacc cggtggtttg
     4801 caccggctcg cgctcggaac tcaacgctcg cgccctcaag ggcttgatgg atgcgctgag
     4861 gatcgagcga gcgcatttga tcggcacttc gatgggggcg cacagcgtca ccgcgtttgc
     4921 actcgccaac ccgacgcgca tcggcaagct ggtgctgatg ggcggcggca cgggcggccc
     4981 cagccagttc gtgccgatgc ctaccgaggg gatcaagctc ttgcaggcgc tctaccgcga
     5041 tccgagcatc gagaatctca agagaatgat ggctgtcttc gtctatgacg ccagcagcct
     5101 taccgaggaa ctgttccagg cccgcctcga caacatgctg cagcggcgcg agcacctcga
     5161 taatttcgtc aagagcctgg ctgccaatcc ccggcagttc accgattacg gtccccgctt
     5221 gtcggaaatc agtgcgccga ccttggtgat ctgggggcgt gacgaccgtt tcgtgccaat
     5281 ggatattggt ctgcgtctgt tatggggcat gcccaacgcc gaactgcata tcttcaacaa
     5341 gtgtggccat tgggcgcagt gggaacatgc ccggaaattc aatgcgatgg tgctggattt
     5401 cctgcgggac tgaagcggcg ctgcactatc ccgagcttcc gtctgcgacg gggcgggcag
     5461 aggaagcgtc gcttcccagg tccgcccgtt agcgcccgaa gtgacggcgt aggcgctcga
     5521 ccgcctcggc cagccggtcg acaccggtgg tgtaggcgaa acggatgtac ttctccggcg
     5581 cgttggcgcc gaaatcgagc cccggcgttg ccgcgacgcc ggcgtcttcg agcagtttgc
     5641 gcgccagtgc aaagctgtcg gcagcgaccg ccgagcagtc gcagtacagg tagaaggcgc
     5701 cttccgggcg ggcgacgacg gagaggccga tggcttccag tgccggtgcg agaaagtcgc
     5761 ggcggcggcg gaactcggcc cgccgttcct cgaggatggc gatggtttcc ggccggaagg
     5821 cggcgagcgc cgcgtactgc gccggtgtcg acgggcagag gacgaggttc tgcgccagct
     5881 tttcgacgtc gcgcacgaag gcttccagaa tcaccatcca gcccagccgc cagccggtca
     5941 tctggaaata cttggaaaag ctgttgatca cccagatgtc gtcgccggct gcgcaggcgg
     6001 tcggcgcgtc gcattcgtag gtgaggccgt ggtagatctc atcgaccagg aaatggccgt
     6061 cgcgggcgcg gcagacctcg gccagcgcgg cgatctcggg cagcgtcaac aaggtgccgg
     6121 tcgggttggc tggcgaggcg acgagcagcc cggcggtgtg ctcgttccag gccgcgccga
     6181 cctgcgccgg ggtcggctgg aaattgctct cgggtccgac cggaatggaa accggccgcc
     6241 cttcgtaggt gcgcaggatg tgccggttgc acgggtagcc gggatcggcg agcaaccatt
     6301 cgcggccggg atcggcaagg caggcaaagg ccaggttgag cgcgccggac gcgccgttgg
     6361 tgacggcgat gcgcgatgcc ggcacggcga cgccgtagcg gtggcggtag aaatcgctga
     6421 ttgcctggcg caactcgggc agtccgagcg cctgggtgta cagcgttttt ccgctattta
     6481 gcgcgtcgac cgcagccgcg acaatcggtg ccggcgtcgc gaaatccggc tcgccgactt
     6541 ccatgtggat gatgtcacgc cccgccgctt caagctcgcg ggcacgggtc agcagttcga
     6601 cgacgtggaa gggagcaatg tcggcgagac gggcagcgga acggggcatt tcatactcca
     6661 gaaagacgaa ggccaccgaa atgatcggtg gccgtc
//
LOCUS       DAOSDX010000028         6536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4265_length_6536_cov_3.332356, whole genome shotgun sequence.
ACCESSION   DAOSDX010000028 DAOSDX010000000
VERSION     DAOSDX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4265_length_6536_cov_3.332356"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLN02_01120"
     CDS             complement(<1..290)
                     /locus_tag="PLN02_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI50951.1"
                     /translation="MIEISAVHKAFNQGQHNEYWALQGIDLRIEAGKVTAFRGPSGSG
                     KTTLLTIVGCLARPTSGRVRLRGEEVSGLPERFLTEIRRRSFGFVFQQFNLIR"
     gene            complement(287..1420)
                     /locus_tag="PLN02_01125"
     CDS             complement(287..1420)
                     /locus_tag="PLN02_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI50952.1"
                     /translation="MRSWLDRQLHLIDFTLGSLARRKARNLGLLAVYTLLVFVLASLS
                     LFASALRSEAAHVLAGSPEVVLQRLVAGRHDLIPPGYLESIGRVRGVQKKEARLWAYY
                     RDPRSGSQLVFMARPDAEVPVGSVMLGTRLAAAAKLKVGDTLAFADYRGQPYPLRVAS
                     LLTPDSELQSATLVLLAEADFRAFFAYPAGHYTDIALSVRNPLEVGNVASKLAAALPD
                     SRPILRDEMLRTYEHIFDWREGILLTVLVGVILTFAIFAWEKAAGLSAEEKREIGILK
                     AIGWETSDVIRMKVWEGLVVSLTAFASGFLAAWVHVFHFGAPLFAPVLKGWSVVYPEF
                     ALFPAVDGLQVATLFFFTVFPYTAAVLVPIWRAAITDPDLVMR"
     gene            complement(1454..2032)
                     /locus_tag="PLN02_01130"
     CDS             complement(1454..2032)
                     /locus_tag="PLN02_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOI50953.1"
                     /translation="MKRHHFLFAIAAALAIFLAAALFLVDREAPASKLPAGGDFVLHA
                     VDGPFDTKTLRGKVLLVYFGYTSCPDICPTTLSTGAQALALLTAEERARVRMVMISVD
                     PERDSLAHLAQYATFFHPALLGVSGSPEEVAAVLRQFGANYRKGPVQPDGSYAIDHTT
                     ATYVVDAEGKLVSTLNFGSTPEQVVAAVRQYL"
     gene            complement(2198..3595)
                     /locus_tag="PLN02_01135"
     CDS             complement(2198..3595)
                     /locus_tag="PLN02_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOI50954.1"
                     /translation="MLDHLIILVYLSIVLYMGYASRKLGSFREFAVSHGNYGWLAIFC
                     TLSASFIGGGFSTGNAAKVFSSGLAYPLALMGFSLQIMLVACFVAPRMHRFPGAISVG
                     DLMEPAYGRRARVITGIFSMLICAGILGAQIGAMGAIFKVFLGIPPLLGILIGAGIVF
                     FYSTLGGMAAVVRTDILQFWLIILGIPLVFIFGVAEVGGWAAFVAGIPASHLEIIGEQ
                     RSLLSFLSLFLVFMFGETLVPPYLQRLLLGRSASDTAKGNFWSAVVSVPFFLIAGAIG
                     LVALQLDPGLQANLALPEVVRRVMPVGLAGLIIAGVLSIVMSSADSFLNASSVALVQD
                     VTRPLRRTPLDERQALNIARLGNLLTGVLALLFALLIPNVLDILVAAYDYWAPSILVP
                     LVAVLMGWRTTPAAFYAAVAAGVGGTLVWQYVLQSPGQIQGFVVGTLLNLVVFTLATR
                     FGALPATQPARSGGE"
     gene            complement(3823..4632)
                     /locus_tag="PLN02_01140"
     CDS             complement(3823..4632)
                     /locus_tag="PLN02_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50955.1"
                     /translation="MNDRKVLCIKWGTRYGPEYVNRLYGMVARQLTPPFDFVCFTDDR
                     RGIRPEVICHDLPDLGCPVPTNAPGKFRKLALWNADLAGLRGTALFLDLDVVVTGPLD
                     ELFAVGRPDEVILARNWIKPLERLGQTSVFRFPIGGHAYLLEEFRADPEGLAGKFQFE
                     QRYVTKRVRGGVRFFPSAWVRHFRLDCLGPWPLRYLRPARLPRDARIVMFPGKPDPAD
                     AIAGRWSDAVAHAPPLTHLAGLFDAQRGQPLGRHLKRYLRPVDWVAEHWRE"
     gene            complement(4629..>6536)
                     /locus_tag="PLN02_01145"
     CDS             complement(4629..>6536)
                     /locus_tag="PLN02_01145"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOI50956.1"
                     /translation="RSGRLTELRRILVIDEGSPGHLVQSRGLARALAAHCRADLVEYR
                     VRLTLRGFLRPLLRLAGTLARRGLPDRLLRLAYQLPAEPPPPAGVIVVSGGRGFHYAV
                     SLARRSGARLVYCGDPAPLPARWCDVVLSPQAVPGHPRSIASGLLLTDIAPGEIAGAG
                     AGLRAAFAQGGGARLAALLIGGDSRSHCYTAADWTQLVEAVNRLGEQGWRWLLTTSRR
                     TPPAVEARLRREIRADWLVGAVWWHAAPERLLPAFLDAAEIVLVTRDSLSMLSEAVAA
                     GKPAVALRPQTVSPAPVIDAALRQAGRERSVREVAFADLATWLQGPDSASENYALPAV
                     DLRLALARATARLLAVNPPPTEKMPVFGIVVPTYNRPAHLIEAIRSLHAQTYPHWKML
                     ICDDASRVDYAPVLPWLVDRRLRMMRLESNGGPNRARNLCIDRAWREGCDHIVFMDDE
                     DRLEPRALEVAAAMLAAHPEAGWLISNTCGDSEPGQRAITAEGRVDWFADYVYGRRLY
                     GAKTHVIALERLAGIRFDDILRGETWHFFQPLAARTQAWAYPFPSRRIRYRDDGTTQT
                     NTRHPQSWREIHSRYARHLQAIRLRPQCSAAYLYTLLELLKTPQRLLRLVLAATGRGP
                     AGRTESPKGTS"
BASE COUNT         1236 a         2125 c         2282 g          893 t
ORIGIN      
        1 cggatcaggt tgaactgctg gaagacgaag ccgaagctgc gccgccggat ttcggtcagg
       61 aagcgttccg gcaagccgga aacctcttcg ccgcgcagac ggacccgacc gctggtcggc
      121 cgcgccaggc aaccgacgat ggtcagcagc gtcgtcttgc cggaaccgct gggaccacga
      181 aaagccgtca ccttgccggc ttcgatacgc agatcgatgc cctgcagggc ccagtactcg
      241 ttgtgctggc cctggttgaa ggccttgtgg acggcggaga tttctatcat cgcattacga
      301 gatcagggtc ggtgatggcc gcccgccaga tcggcaccag gacggcagcc gtatagggga
      361 agaccgtgaa gaagaacagg gtggcgacct gcaggccgtc gacggcgggg aacagcgcga
      421 attccgggta gaccaccgac cagcccttga gtaccggcgc gaagagcggc gcgccgaaat
      481 ggaagacatg cacccaggcg gccaggaatc cgctcgcgaa ggcggtcagc gaaaccacca
      541 gaccttccca caccttcatc cggatcacgt cgctggtttc ccagccgatt gccttgagga
      601 tgccgatttc acgcttttcc tcggccgaca gcccggccgc cttttcccag gcgaagatgg
      661 cgaaggtgag gatgacgcca accagtacgg tgagcaggat gccttcgcgc cagtcgaaga
      721 tgtgctcgta ggtacgcagc atctcgtcgc gcaggatcgg ccgcgagtcg ggcagcgctg
      781 ccgccagttt gctggcgacg ttgccgactt cgagcggatt gcgcaccgac agcgcgatat
      841 cggtgtaatg cccggccgga tacgcgaaga aggcgcggaa atcggcttcc gcgagcaaca
      901 ccagagtcgc cgactggagt tcggaatccg gcgtcagcag gctggcgacg cgcaagggat
      961 agggctgccc gcgataatcc gcgaaagcca gcgtgtcgcc gaccttcagc ttggcggcgg
     1021 cggccagccg cgtgccgagc atgacgctgc cgaccggtac ttccgcgtcg ggacgcgcca
     1081 tgaagacgag ctggctgccg gatcgcgggt cgcgatagta agcccagagc cgggcttcct
     1141 ttttctggac cccgcgcacc cgcccgatgc tttccagata acccggcgga atcagatcgt
     1201 gccggccggc gaccaggcgc tgcaggacga cttccggcga accggcgagg acatgggcag
     1261 cttcgctgcg cagcgcgctg gcaaacagac tcagcgacgc cagcacgaaa accagcagcg
     1321 tatagacggc gagcaggccg agattgcgcg ccttgcgccg ggccagcgag cccagcgtga
     1381 aatcgatcag atgcagttgc cggtcaagcc aggaacgcat cagcggcaca cggcgggaaa
     1441 gtggcgcacc atgtcacagg tactggcgca cggcagcgac gacctgctcc ggcgtgctgc
     1501 cgaaattgag cgtggacacc agcttgccct cggcatcgac gacataggtc gccgtcgtgt
     1561 ggtcgatggc atagctgcca tcgggctgta ccggcccctt gcgatagttc gccccgaact
     1621 ggcgcagcac cgccgccacc tcctccggcg agccgctgac gccgagcagc gcgggatgga
     1681 agaaagtcgc gtattgcgcc agatgggcga gactgtcgcg ttccggatcg acggaaatca
     1741 tcaccatgcg gacgcgcgca cgctcctcgg cggtcagcag agcgagcgcc tgggcgccgg
     1801 tcgacagggt ggtcgggcag atgtcgggac aactggtata gccgaagtag accagcagca
     1861 ccttgccgcg cagggttttc gtatcgaagg ggccgtcgac cgcgtgcagg acgaagtcgc
     1921 cgcccgccgg cagcttgctg gccggtgcct cgcggtcgac caggaacagg gcggcagcaa
     1981 ggaaaatcgc taacgccgcg gcgatggcga acaggaaatg atgacgtttc atgcgcgcgc
     2041 gccccgagcg gacgaccgtg gcaggcgcgg acaccggctc gccccgtcgg ccagttggaa
     2101 gagagaagac atggccgcga tggtagcgac tccgcgaggg cagatgatgc gacaaattgt
     2161 cgcacacgaa ctcaccgcga atgcgcgcta cgccgcctca ttcgccgcca ctgcgtgccg
     2221 gctgggtagc cggaagcgcg ccgaagcgcg tcgccagggt gaataccacg agattgagca
     2281 aggtgccgac gacaaagccc tggatctggc cgggactctg caacacgtac tgccagacca
     2341 gggtgccgcc gaccccggcc gccaccgccg cgtagaaggc ggccggcgtc gtgcgccagc
     2401 ccatcagcac cgccaccagc ggcaccagga tcgaaggcgc ccagtagtcg taagccgcca
     2461 ccaggatatc cagcacattg ggaatcagca gggcgaacaa cagggcgagc acgccggtca
     2521 gcagattgcc caggcgggcg atgttgagcg cctgccgttc gtcgagcggc gtccgccgca
     2581 acggacgcgt gacgtcctgc acgagagcca ccgacgaggc gttgaggaag gaatcggccg
     2641 acgacatgac gatcgacagc acgccggcga tgatcagccc ggccaggccg accggcatca
     2701 cccgccgcac cacttcgggc agcgccaggt tggcctgcag gccgggatcg agttgcaatg
     2761 ccaccagacc gatggcgccg gcgatcagga agaagggcac ggaaacgacg gcgctccaga
     2821 aattgccctt ggcggtatcc gacgcgctac ggccgagcag cagacgctgc agatagggcg
     2881 gcacgagcgt ctcgccgaac atgaagacga ggaacaggga gaggaaggaa agcagcgagc
     2941 gctgttcgcc gatgatctcc aggtgggagg ccggaatgcc ggcgacgaag gcggcccagc
     3001 ccccgacttc ggccaccccg aaaatgaaca ccaggggaat gccgaggatg atcagccaga
     3061 attgcaggat gtcggtcctg accacggccg ccatgccgcc cagtgtcgaa tagaagaaga
     3121 cgatgccggc gccgatcagg atgccgagca agggcggaat gccgaggaag accttgaaga
     3181 tggcgcccat ggcgccaatc tgcgcgccca ggatgccggc gcagatgagc atggaaaaga
     3241 tgccggtgat gactctggcc cgccgtccgt aggccggctc catcaggtcg ccgaccgaga
     3301 tggcgccggg gaagcggtgc atgcgcgggg cgacgaagca ggcgacaagc atgatctgca
     3361 ggctgaagcc catcagcgcc aggggatagg ccaggccgct ggaaaagacc ttggccgcgt
     3421 tgccggtgga aaagccgccg ccgatgaagg acgcggaaag cgtgcagaag atggcgagcc
     3481 agccgtagtt gccgtgcgac acggcgaact cgcggaaact gccgagtttg cggctggcat
     3541 aacccatgta cagcacgatg gacagataga ccaggatgat caggtgatcg agcatgacgg
     3601 agctttcgtt gcgtgacgac ggcaaagaag atcggccacg cccgcggcag ccttgcgccg
     3661 ccagagggtt cgtggccgcg gcgcgcccgc gccggcatgt gcccttgtca tcaccccgga
     3721 aaatcgcaca aacccgaccg cctggcaacc gccgattccc cgatcagcgc atcccctggc
     3781 gattttcgcc gcagcgggac ggccgattgg cgctgctgat gctcactccc gccaatgttc
     3841 ggcaacccag tcgaccggcc gtaaatagcg tttcaggtga cggccaagcg gctgcccgcg
     3901 ctgcgcgtcg aacaggccgg ccaggtgggt caacggtggc gcatgcgcga cggcatccga
     3961 ccagcggccg gcgatcgcgt cggccggatc gggtttgccg ggaaacatga cgatgcgcgc
     4021 atcgcgcggc aagcgcgccg ggcgtaggta gcgcagcggc cacgggccga ggcagtcgag
     4081 gcggaaatgg cgcacccagg cggacgggaa aaagcggacc ccgccgcgca cgcgtttggt
     4141 cacgtagcgc tgctcgaact ggaatttccc ggccaaacct tccgggtcgg cgcggaattc
     4201 ttcgagcagg taggcgtggc cgccgatcgg gaagcggaag accgaagtct ggcccaggcg
     4261 ttcgagcggc ttgatccagt tgcgcgcgag gatcacctcg tcgggccggc cgacggcgaa
     4321 cagttcgtcg agcgggccgg tgaccactac gtcgaggtcg aggaagagcg cggtgccgcg
     4381 caggccggcg aggtcggcgt tccacaacgc gagcttgcga aacttgccag gcgcgttggt
     4441 cggcaccgga caaccgaggt cgggcaggtc gtggcagatc acctcgggcc ggatgccgcg
     4501 ccggtcgtcg gtgaagcaga cgaaatcgaa gggcggcgtg agctggcgcg ccaccatgcc
     4561 atacaggcga ttgacgtatt ccgggccgta gcgcgtgccc cacttgatgc aaaggacttt
     4621 gcggtcgttc atgacgtacc tttcggagat tccgtccgcc cagccggccc cctgccagtc
     4681 gccgccagga caaggcgcag gaggcgctgc ggcgtcttga gcagttcgag cagggtgtaa
     4741 aggtaggcgg ccgaacattg cggacgcagg cggatcgcct gcagatggcg cgcatagcgc
     4801 gaatggattt cgcgccagct ttgcggatgg cgcgtgttgg tctgcgtggt cccgtcgtcc
     4861 cgatagcgga tgcgccggct cgggaaggga taggcccagg cttgcgtgcg cgccgccagc
     4921 ggctggaaga agtgccaggt ttcgccgcgc aggatgtcgt cgaaacggat gccggccagc
     4981 cgctcgaggg cgatcacgtg ggtcttggca ccgtacagtc ggcgcccgta aacgtaatcg
     5041 gcaaaccagt ccacccgccc ttccgccgtg atcgcgcgct gcccgggctc gctgtcgccg
     5101 caggtattgg agatcagcca gccggcctcg ggatgcgccg ccagcatcgc tgccgcgacc
     5161 tccagcgcac gcggttcgag gcggtcctcg tcgtccatga agacgatgtg atcgcagcct
     5221 tcgcgccagg cgcggtcgat gcacaggttg cgcgcccggt tcggaccgcc gttgctttcg
     5281 aggcgcatca tgcgcaggcg gcggtcgacg agccagggca gcaccggcgc gtaatcgacg
     5341 cgcgaagcgt cgtcacagat cagcatcttc cagtgcggat aggtctgcgc gtgcagcgaa
     5401 cggatcgctt cgatcaggtg ggccggccgg ttgtaggtcg gcacgacgat gccgaagacc
     5461 ggcatttttt ccgtcggcgg cgggttgacc gcaagcaggc gggcggtcgc gcgggccagc
     5521 gcaagcctga ggtcgacggc cggcaaagcg taattttccg atgcactgtc cggcccctgc
     5581 agccacgtgg cgagatcggc aaacgccacc tcgcgcacgc tgcgctcgcg ccccgcctgg
     5641 cgcagtgccg catcgatcac cggcgccgga ctgaccgtct gcgggcgtag tgcgacggcc
     5701 ggcttgccgg cggcgaccgc ctcgctgagc atgctcaggc tgtcgcgggt gaccagcacg
     5761 atctctgccg catcgaggaa agccggcagc aggcgttccg gtgcggcgtg ccaccagacg
     5821 gcgccgacca gccagtcggc cctgatttcg cgacgcaggc gggcttcgac cgcaggcggt
     5881 gtacgccgcg aagtcgtcag cagccagcgc cagccctgct cgccaagccg gttgaccgcc
     5941 tcgaccaact gcgtccagtc ggccgccgtg taacaatggc tgcgcgaatc gccgccgatc
     6001 agcagcgccg ccagccgcgc cccgccgcct tgagcgaagg ccgcgcgcag cccggcgccg
     6061 gcaccggcga tttcgcccgg cgcgatgtcg gtgagcaaca ggccgctggc gatgctgcgc
     6121 gggtggccgg gcacggcctg cggactgagc acgacatcgc accagcgcgc cggcagcggc
     6181 gccggatcgc cgcaatagac gagcctcgca ccgctgcgcc gggccaggct gaccgcatag
     6241 tggaagccgc gcccgccgct gacgacgatc accccggccg gcggcggcgg ttcggcgggc
     6301 aactggtagg ccaggcgcag caggcggtca ggcaggccgc gccgggccag cgtgccggcc
     6361 agacgcaaca gcggacgcag gaagccgcgc agggtcaggc ggacccggta ttcgaccaga
     6421 tcggcccggc aatgcgccgc cagcgcccgg gccagcccgc gcgactgcac caggtgaccg
     6481 gggctgcctt cgtcgatgac cagaatgcgt cgcaactcag tcaagcggcc gctccg
//
LOCUS       DAOSDX010000029         6457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4337_length_6457_cov_3.119025, whole genome shotgun sequence.
ACCESSION   DAOSDX010000029 DAOSDX010000000
VERSION     DAOSDX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4337_length_6457_cov_3.119025"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /gene="nifA"
                     /locus_tag="PLN02_01150"
     CDS             <1..1491
                     /gene="nifA"
                     /locus_tag="PLN02_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HOI50957.1"
                     /translation="RESLREVLRVLHEEAGLTRGLIGVVDAESGKLAVHTIYNPDGPD
                     ADTAQYGPGEGIIGLILERPRSIKLMRVADEPRFLNRMRIYEPELPFIAVPIKVGGNL
                     QGVLAVQPEAPEDGLLEERAQFVEMVANLIGQNLRLAMDVAQEKSSLLEERDLLRRTV
                     RHQHGFDSMVGHSAVMRRVFDQARMVAKWNTTVLIRGETGTGKELIANAIHYNSPRAR
                     NMLVKLNCAALPENLLESELFGHERGAFTGAVEARKGRFEQANGGTLFLDEIGEVSAA
                     FQAKLLRILQEGEFERVGGSKTIKVDVRIIAATHRDLETAVEMGDFREDLFYRLNVMP
                     LFLPPLRERIEDIPDIARHLLGKIGNDQKRKLTLTDMAQRRLASHDWPGNVRELENCL
                     ERAAVLSEDGRIDVDLIRFPSSRERSSGRPLRNGGPASFASAPPAAPPAEEVDLADPS
                     LSERERVIAALEQAGWVQAKAARILGMTPRQIAYRIQTLNIEVKQF"
     gene            1592..3061
                     /locus_tag="PLN02_01155"
     CDS             1592..3061
                     /locus_tag="PLN02_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI50958.1"
                     /translation="MQRSPFITSAIVEVVFLNALLFIAGLIGMATHGWLAIDWLWFAC
                     LMAFGIGSGFFYLHKLRVALDPLNHISRIAGEIAQGIIGKRIPPNNRRDELGTVCNNV
                     NAMLDQMESCFGKQRAALKAASENRFGEAIDSQGLCGVFREAVDQGNQSLAILLRNYH
                     QEMRNNLLSRLGQLNEENLLKNMRTIQNDMLGIVAATDALTRLSTANADAAQESNTAI
                     AAVVAAMNRLVDKIEDTGHAIVEFNAHREEIARSVTLIASIADQTNLLALNAAIEAAR
                     AGEHGRGFAVVADEVRKLAEDTIKASTAISGVMQTLQGDAERMLANSGEMRDIASESR
                     ATIGDFDTRFAGVADASSQALEQIHYIHDVSFAALAKVDHFIYKQNGYLAINHGLDSD
                     NARAVEVNEQSCRLGKWLGQDETLTIFGKLGSFRRISDPHRTVHQSMHRAMELLASGW
                     ETDYAIQEKLHGAFEHVESGSDGVIAALDAMVHEKHGSR"
     gene            3187..3516
                     /locus_tag="PLN02_01160"
     CDS             3187..3516
                     /locus_tag="PLN02_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOI50959.1"
                     /translation="MPRPKKHVLVCVQGRPAGHPRGSCQDKGCAATWQAFSDEFTARN
                     LWNSGFQLTNTGCLGPCHLGPTVIVYPEGTMYVGVKPTDVGSIIDEDLMFDQPVERLK
                     APADVWA"
     gene            3521..3790
                     /locus_tag="PLN02_01165"
     CDS             3521..3790
                     /locus_tag="PLN02_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HOI50960.1"
                     /translation="MALVQYEIGDLVYAATDILNDGGMPGIEEEEGLIAAAGSRGVVV
                     QFGHVEADPDQEIYLVRFENGPDGTLGDPVGVLPEELTQELAVSA"
     gene            3793..4326
                     /locus_tag="PLN02_01170"
     CDS             3793..4326
                     /locus_tag="PLN02_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOI50961.1"
                     /translation="MEQIGIFFGTDTGRTRLVAKQIARKLGDLAAAPVNIGRSTLADF
                     LAYERLIVGSPTLGDGELPGDSTGLSQPSWEEFLPQLADADLSGKTIAIFGLGDQQKY
                     PNEFVDAIGLLHDAFVARGATVVGRWPVAGYEFKASRAADGDHFLGLALDQHGQANLS
                     ESRVDTWLEQIKPELLR"
     gene            4323..4628
                     /locus_tag="PLN02_01175"
     CDS             4323..4628
                     /locus_tag="PLN02_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50962.1"
                     /translation="MNTNPWSLPKSRSLRRLLVSLDEQFGEACDIAPDNGADPDIVTL
                     RHHELANLRAHVYLHGQRPGTYGVFFEYPHPVPGILEAEENLPLPKALSSLALHFDA"
     gene            complement(4639..5286)
                     /locus_tag="PLN02_01180"
     CDS             complement(4639..5286)
                     /locus_tag="PLN02_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50963.1"
                     /translation="MADHPYKRTPLPAAPKPRLGAILACLATALAGPFIGFAVVGQVS
                     EAGLLLGLGLSLLPLAGLAVLVRQYHRRLRRLPPALAAEWMQGKLVATAGGPVVTAPW
                     RYACGKNWIELRSDGVLVSRHALLALPGVAEAMGRLWMAGEIGEFFIEWAALGEWSVC
                     DESDGPDTYRVSVRDGGWVHLRRFRGEAAGEVALLDAVRSIGGLPVRVLAEVADD"
     gene            complement(5288..6223)
                     /locus_tag="PLN02_01185"
     CDS             complement(5288..6223)
                     /locus_tag="PLN02_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI50964.1"
                     /translation="MDMLFILHPQHTAFDFAGPAEVIHMAAQAGAPFRLCVAGPEAEC
                     VNSLGVVNRVDPLPETLADGTLVFLLGTEDEPRDYATPAARAIIDWLHAQFDPARHRL
                     ACICSGALLAARAGLLDGRRCTTHHALIGMLRDWAPRAQVLENRMFVEDGPVFTSAGI
                     AGGVDLALAIVERFAGGLLATQVARRMVIYLRRTGADPQLSPWLAHRNHLHPAVHKAQ
                     DLIARDPAADVSVGEIAAQVHVSPRHLSRLFRNEAGIGLLDYRHGLRVELARQLIAQG
                     QSVERAAEGAGFASARDLRRVWKQHAPGTPGQGGG"
     gene            complement(6239..>6457)
                     /locus_tag="PLN02_01190"
     CDS             complement(6239..>6457)
                     /locus_tag="PLN02_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50965.1"
                     /translation="LPWAGLALVVAGVPLALRFWLWRRRGARVIVAGVGAAGLVAELR
                     RLGYAAELPGGASCDTGAADALGLLRPA"
BASE COUNT         1195 a         2393 c         1996 g          873 t
ORIGIN      
        1 cgcgaatcgc tgcgcgaagt gctgcgcgtg ctgcacgagg aagccgggct gacgcgcggc
       61 ctgatcggcg tcgtcgatgc cgaaagcggc aagctcgccg tccataccat ctacaacccg
      121 gacggtccgg acgccgacac cgcccagtac ggaccgggcg aaggcatcat cggcctgatc
      181 ctggagcggc cgcgcagcat caagctgatg cgcgtcgccg acgagccgcg cttcctcaac
      241 cggatgcgca tctacgagcc ggaactgccc ttcatcgccg tgccgatcaa ggtcggcggc
      301 aacctgcagg gcgtcctcgc ggtccagccg gaggcgccgg aagacggcct gctcgaggaa
      361 cgcgcccagt tcgtcgaaat ggtcgccaac ctgatcggcc agaacctgcg cctggcgatg
      421 gacgtcgccc aggaaaagtc ctcgctgctc gaggaacgcg acctgctgcg gcgcaccgtc
      481 cgccaccagc acggcttcga cagcatggtc ggccattcgg cggtgatgcg ccgggtcttc
      541 gaccaggcgc gcatggtcgc caagtggaac accaccgtgc tgatccgcgg cgaaaccggc
      601 accggcaagg aactgatcgc caacgccatc cattacaact cgccgcgcgc ccgcaacatg
      661 ctggtcaagc tgaactgcgc ggcgctgccg gaaaacctcc tcgaatccga actcttcggc
      721 catgagcgcg gtgccttcac cggcgcggtg gaagcgcgca agggccgttt cgagcaggcc
      781 aacggcggca ccctgttcct cgacgaaatc ggcgaagtct ccgccgcctt ccaggccaag
      841 ctgctgcgca tcctgcagga aggcgagttc gagcgggtcg gcggcagcaa gacgatcaag
      901 gtggacgtgc gcatcatcgc cgccacccac cgcgatctgg aaaccgccgt cgaaatgggc
      961 gacttccgcg aggatctgtt ctaccgcctc aacgtcatgc cgctcttcct gccgccgctg
     1021 cgcgagcgga tcgaggacat cccggacatc gcccgccacc tgctgggcaa gatcggcaac
     1081 gaccagaagc gcaagctgac gctgaccgac atggcgcagc gtcggctggc cagccacgac
     1141 tggccgggca atgtgcgcga actggagaac tgcctggaac gcgccgccgt gctctccgaa
     1201 gacggccgta tcgacgtcga cctgatccgc ttcccgagca gccgcgagcg cagctccggg
     1261 cggccgctgc gcaacggcgg gccggcatcg ttcgccagcg cgccgccggc agcgcccccg
     1321 gcggaagagg tcgacctcgc cgatccctcg ctgtcggaac gcgagcgggt gatcgccgcg
     1381 ctcgaacagg cgggctgggt gcaggccaag gcggcacgca tcctgggcat gacaccgcgg
     1441 cagattgcct accgcatcca gacgctgaac atcgaggtca agcagttctg accgcaccgg
     1501 cgcggccgtg ctggcacggc cgccgcgtcc tgccgtaaca ttcgccggca agaatcccgc
     1561 cgtctccgcc tccatccgcc aggaaagcct catgcagcgt tcgcccttca tcaccagcgc
     1621 catcgtcgaa gtcgtctttc tcaacgccct cctcttcatc gccggcctga tcggcatggc
     1681 cacccacggc tggctcgcca tcgactggct gtggttcgcc tgcctgatgg ccttcggcat
     1741 cggtagcggc ttcttctacc tgcacaagct gcgcgtcgcc ctcgacccgc tcaaccatat
     1801 ttcccgcatc gccggcgaaa tcgcccaggg catcatcggc aagcggattc cgccgaacaa
     1861 ccggcgcgac gaactgggca cggtgtgcaa caacgtcaac gccatgctcg accagatgga
     1921 aagctgcttc ggcaaacaac gggccgcact caaggcggcg agcgaaaacc gttttggcga
     1981 ggcgatcgac agccagggcc tgtgcggcgt cttccgcgaa gcggtcgacc agggcaacca
     2041 gtcgctcgcc atcctgctgc gcaactatca ccaggaaatg cgcaacaacc tgctgtcccg
     2101 cctcggccag ttgaacgagg aaaacctgtt gaagaacatg cggacgatcc agaacgacat
     2161 gctcggcatc gtcgccgcca ccgacgccct gacccggctt tccaccgcca acgccgacgc
     2221 cgcacaggaa agcaacacgg cgattgccgc cgtcgtcgcg gcgatgaacc gcctggtcga
     2281 caagatcgag gacaccggtc acgcgatcgt cgaattcaac gcccaccgcg aggaaatcgc
     2341 ccgttcggtg acgctgatcg ccagcatcgc cgaccagacc aacctgctcg ccctcaacgc
     2401 cgccatcgaa gccgcccgtg ccggcgagca cggtcgcggc ttcgccgtcg tcgccgacga
     2461 agtgcgcaag ctggcggaag acacgatcaa ggcgtcgacc gcgatctccg gcgtcatgca
     2521 gacgctgcag ggcgacgccg agcgcatgct cgccaactcc ggcgaaatgc gcgacatcgc
     2581 ttccgaatcg cgcgccacca tcggcgattt cgacacccgc ttcgccggtg tcgccgacgc
     2641 ctcgtcgcag gcgctggagc agatccacta catccacgac gtcagcttcg cggcgctggc
     2701 caaggtcgac cacttcatct acaagcagaa cggctacctg gcgatcaacc acggcctcga
     2761 ctccgacaac gcccgggcgg tcgaggtcaa cgagcagtcc tgccgcctcg gcaagtggct
     2821 gggccaggac gaaaccctca ccatcttcgg caaactcggc agcttccgcc gcatcagcga
     2881 cccgcaccgc acggtgcacc agagcatgca ccgggcgatg gaactgctgg ccagcggctg
     2941 ggagaccgac tacgcgatcc aggagaagct gcacggcgcc ttcgaacacg tcgaatcggg
     3001 cagcgacggc gtcatcgccg cactcgacgc gatggtccac gagaagcacg gcagccgctg
     3061 aaaccctggc ctgcgttgct cttgtcgcga acccgacaag agcaacgcag gccaatccac
     3121 tgaaaacaaa gcactaatta ctggcatcac ctttgctaag gattctccat cgacaaggag
     3181 aatctcatgc caagaccgaa gaaacacgta ctcgtctgcg tccagggccg ccccgccggc
     3241 cacccgcgcg gttcctgcca ggacaagggc tgcgccgcca cctggcaggc cttctccgac
     3301 gagttcacgg cccgcaacct gtggaattcg ggcttccagc tcaccaacac cggctgcctc
     3361 ggcccctgcc atctcggccc gaccgtcatc gtctacccgg aaggcacgat gtacgtcggc
     3421 gtcaagccga ccgacgtcgg cagcatcatc gacgaggacc tgatgttcga ccagccggtc
     3481 gaacgcctga aggcccccgc cgacgtctgg gcctgacatc atggctctcg tccagtacga
     3541 aatcggcgac ctcgtctatg ccgccaccga catcctcaac gatggcggga tgcccggcat
     3601 cgaagaggaa gaaggcctga tcgccgccgc cggctcgcgc ggcgtggtgg tgcagttcgg
     3661 ccacgtcgaa gccgaccccg accaggaaat ctacctggtg cgcttcgaga acggccccga
     3721 cggcaccctc ggcgacccgg tcggcgtgtt gccggaagag ctgacccagg aacttgcggt
     3781 cagcgcctga gcatggagca gatcggcatc ttcttcggca ccgacaccgg ccgcacccgc
     3841 ctggtggcga agcagatcgc caggaaactc ggcgatctcg ccgccgcccc ggtcaacatc
     3901 gggcgcagca cgctcgccga tttcctcgcc tacgagcgcc tgatcgtcgg cagcccgacg
     3961 ctcggcgatg gcgaactgcc gggcgattcg accgggctga gccagccgag ctgggaagaa
     4021 ttcctgccgc aactggccga cgccgacctc tccggcaaga ccatcgccat cttcggcctc
     4081 ggcgaccagc agaagtaccc gaacgaattc gtcgacgcga tcggcctcct gcacgacgcc
     4141 ttcgtcgccc gcggggcgac cgtggttggt cgctggccgg tcgccggcta cgagttcaag
     4201 gcatcgcggg cggccgacgg cgaccacttc ctcggcctgg cgctcgacca gcacgggcaa
     4261 gccaacctca gcgagagccg cgtcgacacc tggctggaac agatcaaacc ggagctgctg
     4321 cgatgaacac caatccctgg tcgctaccca agagccgttc cctgcgccgc ctgctagtca
     4381 gcctcgacga gcagttcggc gaagcctgcg acattgcccc cgacaatggc gccgaccccg
     4441 acatcgtcac cctgcgccac cacgaactcg ccaacctgcg cgcccacgtc tacctgcacg
     4501 gccagcgccc cggcacctac ggcgtcttct tcgaataccc gcatccggta cccggcatcc
     4561 tcgaagcaga ggagaacctg ccgctaccca aggcgctaag cagcctggcg ctgcacttcg
     4621 acgcctgaca ccggcggctc agtcgtcggc cacctcggcc agcacccgga ccggcaagcc
     4681 gccgatgctg cgcaccgcat cgagcagtgc gacctcgcca gcagcttcgc cacgaaagcg
     4741 gcgcagatgc acccagccac cgtcccgcac ggaaacccgg taggtatcgg gaccatcact
     4801 ttcgtcgcac accgaccatt cgcccagcgc cgcccattcg atgaagaatt cgccgatttc
     4861 gccggccatc cacaagcggc ccatcgcctc cgcgacaccg ggcaaggcga gcagcgcatg
     4921 gcgcgacacc agcacgccgt cgctgcgtag ctcgatccag ttcttgccgc aggcgtagcg
     4981 ccagggtgcc gtcaccacgg gtccgcccgc cgtcgccacc agcttgccct gcatccattc
     5041 cgctgccagc gccggcggca gacgacgcag acgccgatgg tattggcgga ccagcaccgc
     5101 cagtccggcc agcggcagca ggctcagccc gaggccgagc agcaggccag cctcgctgac
     5161 ctggccgacc acggcaaaac cgatgaacgg accggccagt gcggtcgcca gacaagcgag
     5221 aatcgccccc aggcgaggct tgggcgcggc cggcagcggc gtgcgcttgt aaggatggtc
     5281 ggccatctca gccgcccccc tgccccggcg ttcccggtgc atgctgtttc cacacccggc
     5341 gcaggtcgcg cgccgaggcg aagccggcgc cctcggcggc gcgctcgacg ctctgcccct
     5401 gggcaatcag ctggcgcgcc aactcgacgc gcaggccgtg gcggtagtca agaaggccga
     5461 tcccggcctc gttgcggaac agccggctga ggtggcgcgg gctgacatgc acctgcgccg
     5521 cgatctcgcc gaccgaaaca tccgctgccg gatcgcgggc gatcaggtcc tgcgccttgt
     5581 gcaccgccgg atgcaggtgg ttgcgatggg ccagccaggg cgacagctgc gggtcggcgc
     5641 cggtgcgccg caggtagatc accatccgcc gcgccacctg cgtcgccagc aaaccgccgg
     5701 caaagcgttc gacgatggcc agcgccaggt cgacgccgcc ggcgatgccg gccgaggtga
     5761 acaccggtcc gtcctcgacg aacatccggt tctccagcac ctgcgcgcgc ggcgcccagt
     5821 cgcgcagcat gccgatcaac gcgtggtgcg tcgtgcaccg gcgaccgtcg agcaggccgg
     5881 cgcgcgccgc cagcaaggcg cccgagcaga tgcaggcgag ccggtggcgg gccgggtcga
     5941 actgcgcgtg cagccagtcg atgatcgccc gcgccgccgg cgtcgcgtaa tcgcgcggct
     6001 cgtcctcggt gccgagcagg aagaccagcg tcccgtcggc cagcgtttcc ggtagcggat
     6061 cgacgcgatt gacgacgccc agcgaattga cgcactccgc ctccggcccc gccacgcaca
     6121 ggcggaaggg cgcgccggcc tgcgccgcca tgtggatgac ctcggccggc ccggcaaaat
     6181 cgaaggcggt gtgctgcgga tgcaggatga acagcatgtc catggcaaaa ctcccgaatc
     6241 aggccggacg caacaagccc agggcgtccg ccgcgccagt gtcgcacgaa gcgccaccgg
     6301 gcagttcggc ggcgtacccc aggcggcgca attcggccac caacccggca gcgccgacgc
     6361 cggccacgat cacccgcgcc ccccgccgcc gccacaacca gaagcgcagc gccagcggca
     6421 cgcccgcgac gaccagcgcc aggcccgccc acggcag
//
LOCUS       DAOSDX010000030         6425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4357_length_6425_cov_3.287127, whole genome shotgun sequence.
ACCESSION   DAOSDX010000030 DAOSDX010000000
VERSION     DAOSDX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4357_length_6425_cov_3.287127"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PLN02_01195"
     CDS             <1..175
                     /locus_tag="PLN02_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HOI50966.1"
                     /translation="TIVTRHAPDFDGATMEMRASSGGNYLSLTCTVVATSKPQLDALY
                     MELTSHPLVKVVL"
     gene            177..794
                     /gene="lipB"
                     /locus_tag="PLN02_01200"
     CDS             177..794
                     /gene="lipB"
                     /locus_tag="PLN02_01200"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOI50967.1"
                     /translation="MSIVVKRLGRVDYAPTFQAMQEFSATRTAETPDELWIVEHPPVY
                     TLGQAGKPEHILRDLGIPTVKIDRGGQVTYHGPGQVVIYLLLDLPRLKIKVRELVTAI
                     EQAVIDLLAAHGVSAERRDGAPGVYVGPAKIAALGLRIRNGCSYHGVSLNVDMDLSPF
                     EAINPCGYPGLQVIQTKDLGIPLTVHEAGEQLSQQLLQQLDKQHG"
     gene            787..1740
                     /gene="lipA"
                     /locus_tag="PLN02_01205"
     CDS             787..1740
                     /gene="lipA"
                     /locus_tag="PLN02_01205"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOI50968.1"
                     /translation="MAETKQKGVKQKGELKTARIPIKIVPVAEPLRKPEWIRVKAGNS
                     AGRFGEIKTMLREKKLHTVCEEATCPNIGECFGRGTATFMILGDICTRRCPFCDVGHG
                     QPLPPNPDEPRELAESVAALKLRYVVITSVDRDDLRDGGAQHFVDVISNVRELSPTTT
                     IETLVPDFRGRMDVALDVLGGALPDVLNHNLETVPRLYKQARPGADYQHSLDFLKQFK
                     ARYPNVSTKSGLMVGLGETDEEILDVMRDLRAHDVEMLTIGQYLAPSGHHLPVTRYVH
                     PDVFKMYEEEAKKMGFTGAACAPMVRSSYWADQQAHSAGVA"
     gene            complement(1742..2266)
                     /locus_tag="PLN02_01210"
     CDS             complement(1742..2266)
                     /locus_tag="PLN02_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HOI50969.1"
                     /translation="MSSAFRLSLLPCLLALGACTVLPTGPSVMVLPGTGRSIESFRAD
                     EAWCKDDAMRQIGGTNAGEAARESAVTSAAVGAAVGALAGAAIGGNSRGAGVGAGVGL
                     LGGTAAGAEHGRASRVGTQRQYDNAYVQCMYAKGHRVPVSGSFSQGRAQRAEDAGIPA
                     PPAGKPPAAPPGVR"
     gene            complement(2280..2624)
                     /locus_tag="PLN02_01215"
     CDS             complement(2280..2624)
                     /locus_tag="PLN02_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50970.1"
                     /translation="MKYAKVFLLCCGLLALTSQEAMAHGRRTHVGVVIGAPLWGPFWD
                     PFPWYPYYPPYPPVVVTPPPPPPVYIEQAEPAPAPASEDYWYYCSSARAYYPDVRSCP
                     EDWVPVLPRSGK"
     gene            complement(2713..3042)
                     /locus_tag="PLN02_01220"
     CDS             complement(2713..3042)
                     /locus_tag="PLN02_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50971.1"
                     /translation="MGLPKRFSDQELEQIVEEATIYMCACPGQVAAQLRSLRELHRYQ
                     MCCATEPENDGSVHRSIAEATERSHAIMEACLEQVLALEGWDRQTLKMPEGLRRKRAE
                     LLERDIS"
     gene            complement(3052..4347)
                     /locus_tag="PLN02_01225"
     CDS             complement(3052..4347)
                     /locus_tag="PLN02_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOI50972.1"
                     /translation="MSVQNLYETKRMSAADAIQVLRDGDTIVVPTGVGEPPSLLTALS
                     DARRNYRGITVSQILPLRKYGYIDPETVDHVRHSAYFFGGATRPGGQEGWIDFVPAYF
                     SELPMLLKRGLTPSDVVFTLASPMDEHGYFSLSLAPDYTMAAIEKARAVVLEVNPNVP
                     FANGDCHIHISRVAALTESSDPLLEVGLPKIGPVQEAIGAHVAEMIPDGATLQIGYGG
                     IPDAVVMQLTHKRDLGIHTEMIGDGIMTLVEAGVVTNQRKNVHRGKMLATFALGSKKL
                     YDFMHRNPALEMHPVDFTNDPFLAGQNDNLHCINATMQIDFLGQCGSESLGPKPYSGT
                     GGQSDFVRAANRSNGGKAFIVLPSTAKDDTISRIVPTLTPGAHVSTSKNDINYVVTEF
                     GVAQLRGKSARQRCEALIAIAHPNFREELRAAAKKLNLL"
     gene            complement(4450..5592)
                     /locus_tag="PLN02_01230"
     CDS             complement(4450..5592)
                     /locus_tag="PLN02_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOI50973.1"
                     /translation="MNEYFAICPRGLEELLLEELRGIGAEELRTTHGGVHFSGDWAVC
                     YRANLESRLATRILWHLVTGPYVKEDDIYRLALRQLWPNHFAVTRTMRVVTTAIKCPL
                     KSLDYVTLRVKDAVCDRFREDFGERPNIETRDPDVSIHVFLTENRCTLYLDTSGRPLW
                     QRGFRKASVDAPLKENLAAGILKMTGWQPGTPLIDPMCGSGTFLLEAVQVALDRAPGL
                     DRGFAFELLKKFDALAWAQIRAAAEARVKPAGPLPIYGSDNDERAVRATRRNLQEAGF
                     ADCVRVELADLLDIEAPAPAGTLLSNPPYGERIGEQDELAAFYPLLGSALKRRWAGWN
                     CFFFTADLRLPKLVGLKPSRKTPLFNGPLECRLFEIRMVAGSNRDR"
     gene            complement(5594..6010)
                     /locus_tag="PLN02_01235"
     CDS             complement(5594..6010)
                     /locus_tag="PLN02_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOI50974.1"
                     /translation="MLVVKTLHIWMVVSWFAGLFYLPRIYVNLAMVPADSVAERERLL
                     LMSGKLYKFMTPLGILAVATGLWLWFAYGFSGAWLHAKTTLVAGLAVYHLYCGRLLGD
                     FNRGRNTKSHVWYRFFNEIPVIVLLIVLFLVVLKPF"
     gene            complement(5998..>6425)
                     /locus_tag="PLN02_01240"
     CDS             complement(5998..>6425)
                     /locus_tag="PLN02_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI50975.1"
                     /translation="APGIYAADRLPLARLEQRLPALSADEDVFTNHIHADDLAAACRA
                     AIRRGAANRAYNAVDDSDLKMADYFDRVAAAFHLPRPPRLPLAQLAQAVSASQLSFMR
                     ESRRIGNRRLKTELKLRLAYPTVDAGIAEALSRRNACSS"
BASE COUNT         1194 a         2083 c         2062 g         1086 t
ORIGIN      
        1 gaccatcgtc acccgccacg cccccgactt cgacggcgca acgatggaaa tgcgcgccag
       61 ctcgggcggc aactacctga gcctgacctg taccgtggtc gccacctcga agccgcagct
      121 cgacgcgctg tacatggagc tgacctctca cccgctggtc aaggtcgtgc tctgacgtga
      181 gcatcgtcgt caaacgcctg ggccgcgtcg actacgcgcc gaccttccag gccatgcagg
      241 agttcagcgc aacgcggacg gccgagacgc cggacgagct gtggatcgtc gagcacccgc
      301 cggtgtatac gctgggtcag gccggcaaac cggaacacat cctgcgcgat ctcggcatcc
      361 ccaccgtcaa gatcgaccgc ggcggtcagg tcacctacca cggccccggc caggtggtga
      421 tctacctgct gctcgatctg ccgcgactga agatcaaggt ccgcgaactg gtcaccgcca
      481 tcgaacaggc ggtcatcgac ctgcttgctg cccatggcgt cagcgccgaa cgccgcgacg
      541 gcgcccccgg cgtctatgtc ggcccggcca agatcgctgc cctcggcctg cgcatccgca
      601 acggctgctc ctaccacggc gtctcgctga acgtggacat ggacctgtcg ccattcgaag
      661 cgatcaaccc ctgcggctat cccggcctgc aagtgatcca gaccaaggat ctgggcattc
      721 cgctcaccgt tcacgaggcg ggcgagcaac tctcgcagca actgctgcag caactggaca
      781 aacaacatgg ctgaaaccaa gcaaaaaggc gtcaagcaaa agggcgaact gaagaccgca
      841 cgcatcccga tcaagatcgt cccggtcgcc gagccgctga ggaagcccga atggattcgc
      901 gtcaaggccg gcaacagcgc cggtcgcttc ggcgagatca agaccatgct gcgcgagaag
      961 aagttgcaca ccgtctgcga ggaagcgacc tgcccgaaca tcggcgaatg cttcggccgc
     1021 ggcaccgcga ccttcatgat cctcggcgac atctgcacgc gccgctgccc gttctgcgat
     1081 gttggccacg gccagccctt gccgcccaac ccggacgagc cgcgcgaact cgccgaatcg
     1141 gtcgccgcac tgaaactgcg ttacgtcgtg atcaccagcg tcgaccgtga cgacctgcgc
     1201 gacggcggcg cccagcactt cgtcgacgtc atcagcaacg tccgtgagct gtcgccgacg
     1261 accaccatcg aaaccctggt ccctgatttt cgcggccgca tggacgtggc ccttgacgtc
     1321 cttggcggcg ccctgcccga cgtcctcaac cacaacctgg aaaccgtgcc gcgcctctac
     1381 aagcaagccc gccccggcgc cgactaccag cactcgctcg acttcctcaa gcagttcaag
     1441 gcccgctacc cgaacgtctc gaccaaatcc ggcctgatgg tcggcctggg cgaaaccgac
     1501 gaggaaatcc tcgacgtcat gcgcgacctg cgcgcccacg acgtcgagat gctgaccatc
     1561 ggccagtacc tggcgccgtc cggccatcac ctgccggtga cgcgctacgt ccacccggac
     1621 gtattcaaga tgtacgagga agaggcgaag aaaatgggct tcaccggcgc cgcctgcgcg
     1681 ccgatggtcc gctcaagcta ctgggcggac cagcaggcgc acagcgccgg cgtcgcctga
     1741 gtcagcgcac gccgggcggc gccgccggcg gtttgcccgc cggtggcgcc ggaattccgg
     1801 cgtcctcggc gcgctgcgcg cgcccctgac tgaaactgcc cgacaccggc acgcggtgtc
     1861 ccttggcgta catgcactgg acataggcgt tgtcgtactg gcgctgcgtg ccaacccgcg
     1921 aagcccgacc atgctcggcg ccggcggcgg ttccgccgag caggccgacc ccggcaccca
     1981 ctccggcgcc gcgactgttg ccgccaatcg ccgcaccagc cagggcgcca accgcggcgc
     2041 cgacggcggc gctggtcacc gccgattcac gcgccgcctc accggcattg gtgccgccga
     2101 tctggcgcat cgcgtcgtcc ttgcaccagg cctcgtcggc gcggaagctt tcgatgctgc
     2161 gaccggtccc cggcagcacc attacgctgg gcccagtggg caagaccgtg caggcaccga
     2221 gcgccagcag gcagggaagc agggataaac ggaatgcgct ggacatggtt ttcagccttt
     2281 catttgccgg agcgcggcag caccggcacc cagtcttcgg gacaggagcg cacatcgggg
     2341 taataggccc gggcgctgct gcagtaatac cagtaatcct cgctggccgg cgccggagcc
     2401 ggttcggcct gttcgatata gacgggcggc ggcggtggcg gcgtgacgac caccggcgga
     2461 tagggaggat agtagggata ccaggggaag ggatcccaga acggccccca gagcggcgct
     2521 ccgatgacca cgccgacatg cgtccggcgt ccatgggcca tcgcttcctg gctggtcagt
     2581 gcgagcaggc cgcaacacag caaaaagact ttggcgtatt tcatcgaaat cccctagcta
     2641 atggagaaag cttaagtgct caaacccgcc ggagccaatt gaaaattgct acagattgta
     2701 attgccgatg gatcaggata tatcccgttc gagcagttcc gctcgcttgc ggcgcagtcc
     2761 ttccggcatc ttcagcgtct gccggtccca gccttcgagc gccaggacct gctccaggca
     2821 ggcttccatg atggcgtggg agcgctcggt ggcttcggca atgctgcggt gcacgctgcc
     2881 atcattttcc ggctcggtcg cgcagcacat ctggtagcgg tgcagttcgc gcaaactgcg
     2941 caactgcgcc gccacctggc ccggacatgc gcacatgtag atggttgcct cttcgacgat
     3001 ctgttccagt tcctggtcgg aaaatcgctt cggcaaaccc atctcagtgc ctcacagaag
     3061 attcagcttc ttcgccgccg cccgcagttc ctcgcggaaa ttgggatggg cgatggcgat
     3121 cagcgcctcg cagcgctgcc tggcgctctt gccgcgcaac tgggcaacac cgaactcggt
     3181 gacgacatag ttgatgtcgt tcttgctggt gctgacgtgc gctcccgggg tcagcgtcgg
     3241 cacgatccgg gaaatcgtgt cgtccttggc cgtcgacggc agcacgatga aagccttgcc
     3301 gccgttcgag cggtttgccg cgcgaacgaa gtcggactgg ccgccggtgc cggaataggg
     3361 cttcggtccc aggctttccg aaccgcactg gccgaggaaa tcgatctgca tggtggcgtt
     3421 gatgcaatgc aggttgtcgt tctggccagc caggaaggga tcgttggtga aatcgaccgg
     3481 gtgcatttcc agcgccgggt tacggtgcat gaaatcgtag agcttcttcg agccaagcgc
     3541 gaaagtcgcc agcatcttgc cgcgatggac atttttacgc tgattggtga cgacgccggc
     3601 ttcgaccagg gtcatgatgc cgtcgccgat catttcggtg tggatgccga ggtcgcgctt
     3661 gtgggtcaac tgcatgacca ccgcgtcggg aatgccgccg tagccgatct gcagggtggc
     3721 gccgtcggga atcatctcgg cgacgtgagc gccgatcgct tcctggaccg ggccgatctt
     3781 cggcaggccg acttcgagca ggggatcgct gctttcggtc agggccgcaa cgcgagagat
     3841 gtgaatgtgg caatcgccgt tggcgaaagg cacattggga ttgacttcga gcaccaccgc
     3901 ccgcgccttc tcgatcgccg ccatcgtgta gtccggcgcc agcgacagcg agaagtagcc
     3961 gtgttcgtcc atcggcgaag ccagcgtgaa gacgacgtcg gaaggcgtca ggccacgctt
     4021 gagcagcatc ggcagctcgg agaaataggc gggaacgaaa tcgatccagc cttcctggcc
     4081 gcccggacgg gtcgcaccac cgaagaaata ggcgctgtga cggacgtgat cgaccgtctc
     4141 cgggtcgatg tagccgtact tgcgcaaggg caggatctgc gaaacggtga tcccgcggta
     4201 gttgcgccgc gcgtcggaaa gcgcggtcag cagactgggc ggttcgccaa cgccggtcgg
     4261 cacgacgatg gtatcgccat ccctgagcac ctggatggcg tcggcagccg acatgcgttt
     4321 ggtttcgtaa agattctgga cggacatgct gatctcctgt gttgtttttt attttgagcc
     4381 ggatggctcg ctccgcattg tcgcggaaaa atccggagag tcaaacacca acgcagcctc
     4441 tgtccagctt cagcgatccc ggttactgcc tgccaccatg cggatctcga acagccggca
     4501 ttcgagcggc ccgttgaaca gcggtgtctt gcggctcggc ttgaggccga ccagcttggg
     4561 cagccgcagg tcggcggtga agaagaaaca gttccagccc gcccagcgcc gcttcagtgc
     4621 actgcccagc agggggtaga aggccgccag ttcgtcctgt tcgccgatgc gttcgccgta
     4681 gggagggttg ctgagcaggg tgccggccgg agcaggcgcc tcgatatcca gcaaatcggc
     4741 gagttcgacc cgcacgcagt cggcgaaacc ggcttcctgc aggttgcggc gggtggcacg
     4801 gacggcacgc tcgtcgttgt ctgaaccgta gatcggcagc ggcccggccg gtttcacccg
     4861 cgcctcggcg gcggcgcgga tctgcgccca ggccagggca tcgaacttct tcagcaactc
     4921 gaaggcgaag ccgcgatcga gaccgggcgc ccggtcgagc gccacctgca cggcttcgag
     4981 caggaatgtg ccgctgccgc acatcgggtc gatcagcggc gttcccggct gccagccggt
     5041 cattttcagg atgccggcgg caaggttttc cttcagcggc gcgtccacgc tcgccttgcg
     5101 gaagccgcgc tgccacagcg gtcggccgga agtatcgaga tacagcgtgc aacggttctc
     5161 ggtcaggaag acgtggatgc tgacgtcggg atcgcgcgtc tcgatatttg ggcgctcgcc
     5221 gaagtcctcg cgaaagcggt cgcagacggc gtccttgacg cgcagggtga cgtagtcgag
     5281 cgacttcagc gggcacttga tcgccgtcgt caccacacgc atcgtccgcg tcaccgcgaa
     5341 atggttcggc cacaactggc gcagcgccag ccggtagatg tcgtcttcct tgacgtaggg
     5401 accggtcacc aggtgccaaa gaatgcgtgt cgccaggcgc gactccaggt tggcgcggta
     5461 gcacactgcc cagtcgccgg aaaaatggac gccgccgtgg gtggtgcgca gttcttccgc
     5521 gccgatgccc cgcagttctt caagcaggag ttcttcgagg ccgcgcgggc agatggcgaa
     5581 atattcgttc atgtcagaaa ggcttgagga caacgaggaa aagaacgatc agcaggacga
     5641 tcaccgggat ttcattgaag aaacggtacc agacgtgcga cttggtgttg cgcccgcggt
     5701 tgaaatcgcc gagcagtcgg ccgcaataca ggtgatagac ggccagaccg gcgaccagcg
     5761 tggtcttggc atgcagccag gcaccggaaa aaccgtaggc aaaccacagc cacagcccgg
     5821 tcgccaccgc caggatgccg agcggcgtca tgaacttgta gagcttgccc gacatcagca
     5881 gcagacgttc gcgctcggca acgctgtcgg ccggcaccat cgccagattg acgtagatgc
     5941 gcggcaggta gaacaggccg gcgaaccagg aaaccaccat ccagatgtgc agggtcttca
     6001 cgacgagcat gcgtttctcc ttgagagggc ctcggcaatg ccggcgtcga ccgtcggata
     6061 ggcgaggcgt agttttaatt ctgttttcag ccggcggttg ccgatgcgcc gagattcgcg
     6121 catgaacgac aactgcgacg ccgagacggc ttgtgccagt tgcgccagcg gcaggcgcgg
     6181 tggccgcggc agatggaagg cagcggcgac gcggtcgaag taatcggcca ttttcaggtc
     6241 cgagtcatcg accgcattgt aggcgcgatt ggcggcaccg cgccggatcg ccgcgcggca
     6301 ggcggcggcg agatcgtcgg cgtggatgtg attggtgaat acgtcctcgt cggcggacag
     6361 cgccggcaac cgctgttcca gccgcgccag cggcagacga tcggcggcgt agataccggg
     6421 cgcgc
//
LOCUS       DAOSDX010000031         6386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4380_length_6386_cov_2.958458, whole genome shotgun sequence.
ACCESSION   DAOSDX010000031 DAOSDX010000000
VERSION     DAOSDX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4380_length_6386_cov_2.958458"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1186
                     /locus_tag="PLN02_01245"
                     /pseudo
     CDS             4..1186
                     /locus_tag="PLN02_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            1190..1384
                     /locus_tag="PLN02_01250"
     CDS             1190..1384
                     /locus_tag="PLN02_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50976.1"
                     /translation="MAKKLEPLILPQSRPRNPLLVPGLTRRAGPHGNTRKAARQQARQ
                     RTREMLDALLAGDVAEFEID"
     gene            1504..2466
                     /locus_tag="PLN02_01255"
     CDS             1504..2466
                     /locus_tag="PLN02_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI50977.1"
                     /translation="MQKALADEIVACLQGERTVYSYYRDRYGIGLLRHHCRQQPGRRA
                     PVAALRRSAYAGLLEKPRLKPLLATCGGSLDEAWLNAADYDAAQMPFILNLAQWGGEP
                     RSKWRWQQTSRPGVNLVLQLNFSSEHQRLFAPLGDVKHLFTYYGHPVARQRHTLAWSR
                     IDIDWASGTALIEEIQNDWLRYADDLEDWLLRQQRAGASDKDVVKYGYRKLPLPLGQS
                     LAYCRHLRERYRPIWAEAMLWAAIAFLREEIGIAHIYYHSEASGRLLKRIGHTAPPRS
                     LYTELPRRFCFQPTADWPDFLLADKANAGLRRQNPAVGMFRLAA"
     gene            complement(2572..3081)
                     /locus_tag="PLN02_01260"
     CDS             complement(2572..3081)
                     /locus_tag="PLN02_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HOI50978.1"
                     /translation="MAKRPLRKTNRTVLIVVEGETEEAFITHLKKLYYRRGMRLSVRI
                     RNAHGHGPQGIIDKLKSVAKTEDFSQRIAVLDADIPLTAAEARWLRKEKVDTVVSVPA
                     IEATLLAILDKPAPDTTEACKKELQKHAPGDPTEASYHEKYFPHEKLEQARGKLAVLD
                     ALIAAVSTE"
     gene            complement(3091..4284)
                     /locus_tag="PLN02_01265"
     CDS             complement(3091..4284)
                     /locus_tag="PLN02_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI50979.1"
                     /translation="MIHALRFSNFFSFADESEISFVLDARASGTESSFASTATDRHLS
                     KLLAVVGANGAGKTNVIKALDFVCWFITQSFFKEGGGALAVEPHLLGTDRASHFELEF
                     EAEHKLYRYTLAVSSERVLSESLQLKTSRLFSTLFTREWDEASGTEKIQQRGFGLPQK
                     QAEKVKPLASLISTAEQFGVETARSIVNALGKRRSNVGPAGRKAFRGMHDVMNATQFF
                     FDSEQHRQKMTTLLNQWDFGLQDVQIETLRSMQDGKEEEVLVPFGIHRDGEREMKLPM
                     FAESSGTQAAFVLLASLLPILAEGGVVIFDELESDLHPLMLEPILNLFISPKSNPHNA
                     QIIFTCHSLEILNLLRKGQIMLVEKTEHCRSEAWRLSEMEGVRADDNFYAKYMAGTYG
                     AVPML"
     gene            complement(4410..5837)
                     /gene="creC"
                     /locus_tag="PLN02_01270"
     CDS             complement(4410..5837)
                     /gene="creC"
                     /locus_tag="PLN02_01270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HOI50980.1"
                     /translation="MNISVRLFFGYFLVVGLAAWFVLYTFVKESEPSIRQATEESLVD
                     SANLLAELAAGELAAGRIEDGAFATAVAAALQRHPGAEIYGIKKDSVDLRIYLTDAHG
                     VVVYDSAGQARGADFSQWKDVARVLRGEYGARQSRYNPDDPTTSVMHVAAPVRRDGQL
                     IGVISLAKPMTSIAPFVERATDRVRASGLTLLLATAVIGLVFTAWLSWSIHRLRDYAR
                     AVSEGQKAEPPSGGGRQLSELAQALATMREKLEGKQYVERYVQNLAHEMKSPLTAVIG
                     AAEILEDELPAEDRRRFAASIGEQAGRLSDIIERMLQLARVEQLQAPGAKAAVDLAEL
                     LKTAVADRQLALAARHLHCVIDAEAGITLNGDAFLLRQAVLNLLDNAIEFSPDGGEIS
                     LTLRPTAEAAEIRVRDHGAGAPDYALAQLFNRFYSLPRPATGKKSTGLGLALVREVAR
                     LHGGEASFANHPDGGGEARLTLARG"
     gene            complement(5840..>6386)
                     /gene="creB"
                     /locus_tag="PLN02_01275"
     CDS             complement(5840..>6386)
                     /gene="creB"
                     /locus_tag="PLN02_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HOI50981.1"
                     /translation="GVDFVVLDVGLPDITGFDVCRALRRDSDLPVLFLTARSDEVDRI
                     VGLELGGDDYVTKPFSPREVASRVRAILRRLHANGESRGQAEKPAKFAVDRDGLRIAY
                     HGHWLALTRYEYLLLALLLERPGRILSRPQIMAAVWQDDGESLERTVDTHIKTLRGKL
                     RAVRDDEVILTHRGLGYSLAA"
BASE COUNT         1038 a         1964 c         2194 g         1190 t
ORIGIN      
        1 gaactgaaca aggggcgcgt cccggtcaag gtgtggacgc gcgatatcga agctgcggcc
       61 atgcagcagt tgctgaacgt ggccaatctg ccgatcgtgc atggccatgt cgcggcgatg
      121 ccggacgtgc atgcgggcat cggcgccacc gtcggcagcg tcattccgac gcggcaggcg
      181 atcattccgg cggcggtcgg cgtggacatc ggttgcggca tgaacgcggt gcgcacgacg
      241 ctgcgtgccg gggacttgcc ggacagcctg cgcgcgctgc gtctgggcat cgaaggcagc
      301 gtgccttgcg gcttcgagca gcacgcggcg agccggatgc gcggcagcgc cttgcagcgg
      361 gtcgggcggg tgctcgacca gcggctggat gcgattgtcg gcaagcatcc ggggttgatg
      421 aagatgcagc gccgtttcaa cgaaacctgg ctgtgccagc tcggcacgct gggcggcggc
      481 aatcacttca tcgagctgtg cctggacgag gccggcgcgg tgtgggtgat gctgcattcc
      541 ggctcgcgcg gcatcggcaa cgtcatcggt cgctatttca tcgccgccgc gcagaaggac
      601 atgcagcgtc accgcatcca tctgccggac cgcgacctcg cgtatttcag cgagggctcg
      661 gacctgttcg acgattacgt cgaggcggtg gagtgggcgc aggattacgc gctgctcaac
      721 cggcagcaga tgctggcggc ggtggtcgag gtgttgaaga agcagttgcc ggctggagag
      781 tgaggcgatc aactgccacc acaactatgt ggcgcgcgag cggcatttcg gcgagaacct
      841 gttcatcacc cgcaagggcg ccatttcggc gcaggcgggc gaactgggca tcatccccgg
      901 cagcatgggg gcgcgctcgt acatcgtgcg cgggctgggc aacgccctgt cgttctgctc
      961 gtgcgcgcac ggggcggggc ggcggatgag ccgcagcgcg gccaagcggc gcttttcccg
     1021 tttcgatctg gaagaccaga cggcgggcat cgagtgccgc aaggacagcg gcgtcgtcga
     1081 tgaaattccg gcggcgtaca aggacatcga tcaggtgatg gacaaccagc gcgatctggt
     1141 ggaagtggtc cataccctga agcaggtcct gtgcgtgaag gggtgagcga tggccaagaa
     1201 acttgaaccg ctgatcctgc cgcaaagccg gccgcgcaat ccgctgctgg tgcccggact
     1261 gacccgccgc gccggcccgc acggcaacac ccgcaaggcc gcccgccagc aggcgcggca
     1321 gcgcactcga gagatgctcg atgcgttgct cgccggcgac gtggcggagt ttgaaatcga
     1381 ttgaaggaca aggccgcgac ccagccggtc gcggcgcatt ttttaccaag gagaaaacca
     1441 tgactgacct ttcccagccc ccgctacgca cctcattccc gaatgcacct cccggagtca
     1501 tccatgcaaa aagcactcgc cgacgagatc gtcgcctgcc tgcagggcga acgcaccgtc
     1561 tattcctatt accgtgaccg ctacggcatc ggcctgttgc ggcatcactg ccgccagcag
     1621 ccggggcgcc gcgcgccggt cgccgccttg cgccgttccg cctacgccgg cttgctcgaa
     1681 aagccgcgcc tgaagccctt gctcgccacc tgcggcggta gtctcgacga agcctggctc
     1741 aacgccgccg attacgatgc ggcgcagatg cctttcatcc tcaacctcgc ccagtggggc
     1801 ggcgagccgc gcagcaaatg gcgctggcag caaacctcgc ggccgggcgt caatctggtg
     1861 ctgcaactca atttcagtag cgagcaccag cgccttttcg cgccgctcgg cgacgtcaag
     1921 cacctgttta cctactacgg ccacccggtt gcccgccagc gccacacgct ggcctggtcg
     1981 cgcatcgaca tcgactgggc gagcggtacg gcgctgatcg aggaaatcca gaacgactgg
     2041 ctgcgctacg cggacgatct ggaggattgg ttattgcgac aacagcgggc cggtgccagc
     2101 gacaaggacg tcgtcaagta cggctaccgc aagctgccgt taccgctcgg ccagtcgctc
     2161 gcctactgcc gccacttgcg cgaacgctac cggccgatct gggccgaagc catgctgtgg
     2221 gcggcgattg ccttcctgcg cgaggaaatc ggcatcgcgc acatctacta ccacagcgaa
     2281 gccagcggcc gcctgctcaa gcgcattggc cacacggcac cgccgcgctc gctgtacacc
     2341 gaactgccgc gccgcttctg cttccagcca accgccgact ggccggattt cctgctcgcc
     2401 gacaaggcca acgccgggct gcggcggcag aatccggcgg tggggatgtt tcggttggcg
     2461 gcgtagggag ggagaggttc cgcgctggtt ctcttgtgtg acgccagaaa gttctacatg
     2521 gattcgatca gcgctgtgta gaacttgccg gaagcgtttc cgggcgtgcg gctattcggt
     2581 gctgacggcg gcgatcaggg catcaagcac cgcaagcttg ccgcgcgcct gttcgagctt
     2641 ctcgtgcgga aagtactttt cgtgatagct ggcctcggtc gggtcgccgg gggcatgctt
     2701 ttgcaattcc ttcttgcagg cttcggtagt gtccggcgct ggcttgtcca ggatcgccaa
     2761 cagggttgct tcaatcgcgg gcacggaaac gacggtgtcg actttttcct ttcgtagcca
     2821 acgcgcttcc gctgccgtga gcggaatgtc ggcatccagc acggcaatgc gttggctgaa
     2881 atcttccgtc ttggcgaccg acttcagctt gtcgatgatg ccctgcgggc catgcccgtg
     2941 ggcattcctg atcctgacgg acagtcgcat cccccggcgg taatagagct ttttgaggtg
     3001 agtgatgaag gcttcttccg tttcgccctc gaccacgatc agcacggtcc ggttggtttt
     3061 gcgcagcgga cgctttgcca tgtgccggtc tcacagcatg ggcacggcgc cgtaagtgcc
     3121 ggccatgtac ttggcgtaga aattgtcatc ggcgcgcacg ccttccattt ccgacaggcg
     3181 ccaggcttcg ctgcggcagt gttcggtctt ctcgaccagc atgatctggc ctttcctgag
     3241 caggttgagg atttccagcg agtggcaggt gaagatgatc tgggcgttgt gcggattgct
     3301 cttcgggctg atgaacaggt tcaggatcgg ctccagcatc agcgggtgga ggtcggattc
     3361 cagttcgtca aagatcacca cgccgccttc cgccaggatg ggcagcaggc tggcaagcaa
     3421 cacgaaagcc gcctgggtgc cgctggattc ggcaaacatg ggcagtttca tttcccgttc
     3481 gccgtcgcgg tggatgccaa aaggcaccag gacctcttct tccttgccgt cctgcatgct
     3541 gcgcaaggtc tcgatctgga cgtcctgcaa gccgaaatcc cactggttga gcagcgtcgt
     3601 catcttctgg cgatgttgtt cgctatcgaa gaagaattgg gtggcgttca tgacatcgtg
     3661 catgccgcga aaggccttgc gaccggccgg gccgacattg ctccggcgct tgcccagggc
     3721 gttgacaatg ctccgggcgg tttccacgcc gaactgctcc gccgtggaaa tcagcgaggc
     3781 gagcggcttg accttctcgg cctgtttctg cggcaggccg aaaccgcgtt gctggatttt
     3841 ctccgtgccg ctggcttcgt cccattcgcg tgtgaacagc gtgctgaaca ggcggctggt
     3901 cttgagttgc agggattcgg aaagcacgcg ttcgctgctg actgcgagcg tgtagcggta
     3961 cagcttgtgt tcggcttcaa actccagttc aaagtggctg gcgcgatctg tgccgagcag
     4021 atggggctcg acggccaggg cgccgccgcc ctccttgaaa aaggactggg tgatgaacca
     4081 gcagacgaaa tccagcgcct tgatcacgtt ggttttaccc gcgccgttgg caccgacgac
     4141 cgccagcagc ttgctcaggt ggcggtcggt ggcggtcgag gcaaagctgc tctcggtgcc
     4201 gctggcgcgc gcatccagga cgaaagagat ttcgctttcg tcggcgaagg agaagaagtt
     4261 ggagaagcgc agggcgtgga tcaatttgtt gctcggctgt tttcaacaat atgttgttga
     4321 ttgtgcctta gtgtgcggaa ttttgcacgc tgctcgtatg agcacgggtg accattttcg
     4381 tggcgccacg aaaatggtcg cttacagggc tatccgcgcg ccagcgtcag ccgtgcttcc
     4441 ccgccgccat ccggatggtt ggcaaagctg gcctcaccac catgcagcct tgccacctcg
     4501 cgcaccagcg ccagccccag cccggtgctt ttcttgccgg tcgccggccg gggcagggag
     4561 tagaagcggt tgaagagttg cgccagggcg tagtcggggg cgccggcgcc gtggtcgcgg
     4621 acgcggattt cggcggcttc ggcggttgga cgtagagtca gcgagatttc gccgccgtcg
     4681 ggcgagaatt cgatggcgtt gtcgagcagg ttgagcacgg cctggcgcag caggaaggcg
     4741 tcgccgttga gggtgatgcc cgcttcggcg tcgatgacgc agtgcaggtg gcgagcggcg
     4801 agcgccagtt ggcgatcggc gacggcggtt ttcaggagtt cggcgaggtc gaccgcggcc
     4861 ttggcgccgg gcgcttgcag ttgttcgacg cgggcgagtt gcagcatgcg ctcgatgatg
     4921 tcgctcagtc gtccggcctg ttcgccgatg ctggcggcga agcggcggcg gtcttcggcc
     4981 gggagttcgt cctcaaggat ttcggcggcg ccgatcaccg cggtcagcgg gcttttcatc
     5041 tcgtgcgcca ggttctgcac gtagcgttcg acgtactgct tgccttcgag cttttcgcgc
     5101 atcgtcgcca gcgcctgcgc cagttcggag agttggcggc cgccgccgct cggcggttcg
     5161 gccttctgcc cttcgctgac ggcgcgggcg tagtcgcgca ggcggtggat cgaccagctc
     5221 agccaggcgg tgaataccag gccgatcacc gccgtcgcca gcagcagcgt caggccggag
     5281 gcgcgcacgc ggtcggtggc gcgctcgacg aagggggcga tcgaggtcat cggcttggcc
     5341 agcgagatga cgccgatcaa ctggccgtcg cggcgtaccg gcgcggcgac gtgcatcacc
     5401 gaggtggtcg ggtcgtccgg gttgtagcgg ctctggcggg cgccgtattc gccgcgcagg
     5461 acgcgggcga cgtccttcca ctgcgagaag tccgcgcccc gcgcctggcc ggccgagtcg
     5521 tagacgacga cgccgtgtgc gtcggtgagg tagatgcgca ggtcgacgct gtccttcttg
     5581 atgccgtaga tttcggcgcc aggatggcgt tgcagcgcgg cggcgacggc cgtggcgaag
     5641 gcgccgtcct cgatgcgccc ggcggcgagt tcgccggccg ccagttcggc gagcaggttg
     5701 gcgctgtcga ccaggctttc ctcggtggcc tggcggatgc ttggctcgct ctccttgacg
     5761 aaggtgtaca gcacgaacca ggcggccagg ccgaccacca ggaaatagcc gaaaaacagg
     5821 cggaccgaga tgttcacgct caggctgcca ggctgtagcc gaggccgcga tgggtgagga
     5881 tgacctcgtc gtcgcgcacc gcgcgcagct tgccgcgcag cgtcttgatg tgggtgtcga
     5941 cggtgcgctc caggctttcg ccgtcgtcct gccagaccgc cgccatgatc tgcgggcggc
     6001 tcaggatgcg ccccgggcgt tcgagcagca gcgccagcag caggtattcg tagcgggtca
     6061 gcgccagcca gtggccgtgg taggcgatgc gcaggccgtc acggtcgacg gcgaatttcg
     6121 ccggtttttc ggcttggccg cgagattcgc cgttggcgtg caggcggcgg aggatggcgc
     6181 gtacccgcga agccacttcg cgcgggctga acggtttggt cacgtaatcg tcgccgccca
     6241 gttcaaggcc gacgatgcgg tcgacctcgt cggaacgggc ggtgagaaag agcaccggca
     6301 ggtcggagtc gcggcgcagc gcgcggcaga cgtcaaagcc ggtgatgtcg ggcaggccga
     6361 cgtcgaggac gacgaaatcg acgccg
//
LOCUS       DAOSDX010000032         6369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4406_length_6369_cov_3.246753, whole genome shotgun sequence.
ACCESSION   DAOSDX010000032 DAOSDX010000000
VERSION     DAOSDX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_6369_cov_3.246753"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2469)
                     /gene="pepN"
                     /locus_tag="PLN02_01280"
     CDS             complement(<1..2469)
                     /gene="pepN"
                     /locus_tag="PLN02_01280"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOI50982.1"
                     /translation="MNRETPPTIYLKDYTPPAWWIDTVDLDFVIETGATTVTARLALR
                     RNPAVAAQPLVLDGEELETLGVSVDGNAASYTATANSLTLTDLPDTCTLTTVVRIDPD
                     KNTRLSGLYRSKDGYFTQCEAQGFRRITWFLDRPDVMSVYTVTLHADKGAFPVLLANG
                     NPVAAGDEADGRHWAKWADPFKKPAYLFAVVAGKLDVLRDSFRTASGREVQLAIYVEP
                     GKLDQCPHAMAALQKSMRWDEERFGLECDLDHYMIVAVGDFNMGAMENKGLNIFNTKY
                     VLARPDVATDVDFENIDRVVAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFGAD
                     LHNRQTARIREVRGLRAAQFPEDAGPMAHPIRPASFVEINNFYTATVYEKGAEVIRMI
                     QTLIGRDAFRAGMDEYFRRHDGQAVTCEDFVAAMAAASGFDFTQFMRWYNQPGTPKVV
                     VDGHYDPLERSYTLTCTQSNPRASDDAPALIPIRVALFTEAGELIAGSERTLQLTANS
                     QSFVFNDIGDEPVRSLVRDFSAPVNLEFDYTPEQLTLLLAFETDPFNAWEAGQRLAST
                     LILGAAAEIAAGRTPQWPDSFTAACRRLLQTQRERGSAFVAEALTLPGETTLAEQLEI
                     VDPDALHAARNGLRRHLAEQLEGEFAGLYAALTVKEAYAPDSEQAGRRALRNLCLSYL
                     LELDSDALRRLAYAQFREADNMTDQFAALAALANVNAAVCVQREEALAEFYDRWKDEA
                     LVVDKWLQAQSTSRRPDTLATVKALTQHPAFDAGNPNKIYALIRAFGANLVRFHAADG
                     SGYAFMAERILLLHDKNPQVASRLA"
     gene            2653..4650
                     /locus_tag="PLN02_01285"
     CDS             2653..4650
                     /locus_tag="PLN02_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI50983.1"
                     /translation="MFQMSEPARRLLLACGCLAATNVFAEDERGLAQVVVTATRTEQS
                     IREVPAAVSVIDRERIEASGATTVDQVLQGVPGVYAARMDASSPNRIAQTYSRGLPGN
                     SRTLVLLDGIPMNVLYDGQVDWSQLSTQDVERVEVVRGASSGLYGANAMGGVINVLSR
                     APEPGTKTRLGVEYGSLDSKRLLASHSQRFGATSIRVSASHFASDGYDMWRSDTTIPA
                     ANRDKIGTVKDNAGVKLLHEIDAQSFVEAGVSYLKDEATGFYHPGAPGYQPSKREQYV
                     PTVRYVRDDDSSNTSVALYGRFGKQWADTLNPATYATISEKGRYEDRSVGFNAQHSRA
                     LGSIHRVTLGLDFLDGDVDNHFSYPGSSRLRDTRGDLRRYGVFIQDEMALTARWKLNL
                     AGRYDHWTTGGNQVDSGVGQPSTSYRERSAGDFSPKLATLFKVNDAFNLRASAGRAFN
                     LPDMFNLYANTRRGTTTYWANPDLQPETVKAYDLGADYYFGRRGYVKATLYYNDAKDF
                     IYSVRRDATNVDKINVGAVTTRGLELEAAYRPSEALALSASFTYNRSRIEENASNPAL
                     EGKSLTSVPRRQGQVRADYRWPGGATLSAAVNRVGKRYADDINSKSYKAYTTLDRSAS
                     YPLGKTFGARLNVVNVGDKVYDGIGYLAPGRTVSAGLYASF"
     gene            4736..5389
                     /locus_tag="PLN02_01290"
     CDS             4736..5389
                     /locus_tag="PLN02_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOI50984.1"
                     /translation="MRPSSLPPSSPAAARWPGIVLVLAVHGLALWVLLAQRLLPLPEE
                     ASTLMVNFIAPPAPQQTAPKPAPRPPEPKPLPKPAPHQLVAEAPVLSPAEPVAPPPPP
                     APPVEVAPPAPPLPLPQRPVALGGELSVVCPERQAPAYPPMSRRLGETGKVVLRVTLD
                     ERGNVARASIERSSGHPRLDEAALGAVRGWRCTPARRNGQPVEATALQPFNFVLEGN"
     gene            5393..6103
                     /locus_tag="PLN02_01295"
     CDS             5393..6103
                     /locus_tag="PLN02_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOI50985.1"
                     /translation="MQPAVVEGLGFAHFLAQADGVGRVVLGLLLLLSVASWYLIVTKW
                     LANYLAQRQASAFLARFWQGGSRSGLGQRLRNEKIDNAFARLALNAIEAAEEAAAGDD
                     NLATAGGLAEYLTRTLNNTIDQEAALSEHGLTVLASAGSAAPYIGLFGTVWGIYHALV
                     QIGLSGQGTLDKVAGPVGEALIMTALGLAVAIPAVLAYNAFSRRNRLWLARLEGFAHD
                     LYVLLTVKNEAAGGARGG"
     gene            6103..>6369
                     /locus_tag="PLN02_01300"
     CDS             6103..>6369
                     /locus_tag="PLN02_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOI50986.1"
                     /translation="MAAGDFRRYRGRADGPKAEMNVVPLVDVMLVLLVIFIVTAPLLT
                     HAVKIDLPKASSAVNLTKPEHIEFGLRESGEMFWNGEPVTLEELS"
BASE COUNT          988 a         2130 c         2204 g         1047 t
ORIGIN      
        1 cgccagccgc gaggccacct gcggattctt gtcgtgcagc agcaggatgc gttcggccat
       61 gaaggcatag ccgctgccgt cggcggcatg gaagcggacc aggttggcgc cgaaggcgcg
      121 gatcagcgcg tagatcttgt tcgggttgcc ggcgtcgaag gccgggtgtt gcgtcagcgc
      181 cttcaccgtc gccagcgtgt cgggccggcg gctggtcgat tgcgcctgca gccacttgtc
      241 gacgaccagc gcttcgtctt tccagcggtc gtagaactcg gccagcgcct cctcgcgctg
      301 cacgcagact gcggcgttga cgttggccag cgcggccagt gcggcgaact ggtcggtcat
      361 gttgtcggct tcgcggaact gcgcgtaggc cagccggcgc aaggcgtcgc tgtcgagttc
      421 gaggagatag gacaggcaca ggttgcgcag cgcgcggcgg ccggcttgtt cgctgtccgg
      481 ggcgtaggct tccttgacgg tcaacgcggc gtaaaggccg gcaaactcgc cttcgagttg
      541 ttcggcgagg tggcggcgca ggccgttgcg ggcggcgtgc agggcgtccg gatcgacgat
      601 ctccaactgt tcggcgagcg tcgtttcgcc gggcagggtg agcgcttcgg cgacgaaggc
      661 ggagccgcgc tcgcgctgcg tctgcagcag gcggcggcag gcggcagtga agctgtccgg
      721 ccactgcggg gtgcggccgg cggcgatttc ggcggcggcg ccgaggatca gtgttgatgc
      781 caggcgctgc ccggcttccc aggcgttgaa cgggtcggtc tcgaaggcca ggagcagggt
      841 cagctgttcc ggcgtgtagt cgaactcgag attgaccggg gccgagaagt cgcgcaccag
      901 cgagcgcacc ggctcgtcgc cgatgtcgtt gaagacgaag gactggctgt tggcggtcag
      961 ttgcagggtg cgttcgctgc cggcgatcag ttctccggct tcggtgaaca gcgcgacgcg
     1021 gatcgggatc agcgccggcg catcgtcgct ggcgcgcggg ttgctctgcg tgcaggtcag
     1081 cgtgtagctg cgttcgagcg ggtcgtaatg gccgtcgacg acgaccttcg gcgtgcccgg
     1141 ctggttgtac cagcgcatga actgggtgaa gtcgaaacca ctggcggcgg ccatcgcggc
     1201 gacgaaatcc tcgcaggtca ccgcctgccc gtcgtggcgg cggaagtatt cgtccatgcc
     1261 ggcacggaag gcgtcgcggc cgatcagcgt ctggatcatg cggatgactt cggcgccctt
     1321 ttcatagacg gtggcggtgt agaagttgtt gatctcgacg aagctggccg ggcggatcgg
     1381 gtgcgccatc ggcccggcgt cttccgggaa ctgcgcggcg cgcaggccgc gcacctcgcg
     1441 gatgcgggcg gtctgccggt tgtgcaggtc ggcgccgaat tcctggtcgc ggaacacggt
     1501 caggccttcc ttcaaggaga gctggaacca gtcgcggcag gtgacgcggt tgccggtcca
     1561 gttgtggaag tattcgtgcg cgacgacccg gtcgatgttc tcgaaatcca cgtcggtcgc
     1621 aacgtccggg cgagcgagca catacttcgt gttgaagatg ttgagcccct tgttctccat
     1681 cgcgcccatg ttgaagtcgc cgacggcgac gatcatgtag tggtcgaggt cgcattcgag
     1741 gccgaagcgt tcttcgtccc aacgcatcga tttctgcagc gccgccatcg cgtgcgggca
     1801 ctggtcgagc ttgcccggtt cgacgtagat ggccagttgc acctcgcgcc cggacgcggt
     1861 gcggaagctg tcgcgcagca cgtcgagctt gccggcaacg acggcgaaca ggtaggccgg
     1921 cttcttgaac gggtcggccc atttggccca gtggcgaccg tcggcctcgt cgccggcagc
     1981 gaccgggttg ccgttggcga gcaggaccgg gaaagcgccc ttgtcggcgt gcagggtcac
     2041 ggtgtagacc gacatcacgt cggggcggtc gaggaaccag gtgatgcgcc ggaaaccctg
     2101 cgcctcgcac tgcgtgaagt agccgtcctt ggagcggtag aggccggaca ggcgggtgtt
     2161 cttgtcgggg tcgatgcgca cgacggtggt cagcgtgcag gtgtcgggca ggtcggtcag
     2221 cgtcaggctg ttcgccgtcg cggtgtagga cgcggcgttt ccatccacgc tgacaccgag
     2281 cgtttccagt tcctcgccgt cgagcaccag cggctgtgcg gcgacggctg ggttgcggcg
     2341 cagcgcgagg cgggcggtaa ccgtggtggc gccggtttcg atgacgaaat cgaggtcgac
     2401 ggtgtcgatc caccaggccg gcggggtgta gtccttgagg taaatggttg ggggcgtttc
     2461 gcggttcatc gtgtgcaggt tccggagaga agccggaaat gatacgtgaa gtggatgacc
     2521 cactgtcgcc ggcttgcccg gcgggatgcg gcaatttgcc acattccggg gtgccggggg
     2581 gctccgtata gtccgcccct gcacggcgga cggcagggga gccggccgct gaagaatctc
     2641 agggggaaaa ccatgttcca gatgtccgag ccggcccgcc gcctgctgct ggcctgcggc
     2701 tgccttgccg cgaccaatgt ttttgccgaa gacgaacgcg gcctcgccca ggtcgtggtc
     2761 actgcgaccc gcaccgagca gtcgatccgc gaggtgccgg cggcggtttc ggtgatcgac
     2821 cgcgaacgca tcgaagcttc cggcgccacc accgtcgacc aggtgctgca gggcgtgccg
     2881 ggcgtttacg cggcgcgcat ggacgcctcg tcgccgaacc gcatcgccca gacctatagc
     2941 cgcggcctgc ccggcaacag ccggaccctg gtgctgctcg acggcattcc gatgaacgtg
     3001 ctctacgacg gccaggtcga ctggtcgcaa ctgagcaccc aggacgtcga gcgcgtcgaa
     3061 gtggtgcgcg gtgccagctc cgggctctac ggcgccaacg cgatgggcgg cgtcatcaac
     3121 gtgctgtcgc gcgcccccga accgggcacg aaaacgcggc tcggcgtcga atacggcagc
     3181 ctcgacagca agcgcctgct ggcctcgcat tcgcagcgtt tcggtgccac ctcgatccgg
     3241 gtttcggcca gccatttcgc cagcgacggc tacgacatgt ggcggtcgga caccaccatt
     3301 ccggccgcca accgcgacaa gatcggtacc gtcaaggaca acgccggggt caagctgctg
     3361 cacgagatcg acgcccagtc ctttgtcgag gccggcgtca gctacctgaa ggacgaagcc
     3421 accggtttct accatccggg ggcgccgggt taccagccgt cgaaacgcga gcaatacgtg
     3481 ccgaccgtgc gctatgtccg tgacgacgac agttcgaata cctcggtcgc gctttacggg
     3541 cgtttcggca agcagtgggc ggacacgctg aacccggcga cctacgcgac gatctccgag
     3601 aaaggccgtt acgaggaccg cagcgtcggc ttcaacgccc agcattcccg cgcgctgggt
     3661 tccatccacc gggtcacgct gggcctcgat ttcctcgacg gcgacgtcga caaccatttc
     3721 tcctatccgg gcagcagccg cctgcgcgat acgcgcggcg acctgcgccg ctacggcgtc
     3781 ttcatccagg acgagatggc actgacggcg cgctggaaac tcaacctcgc cggtcgctac
     3841 gaccactgga ccaccggcgg caaccaggtc gatagcggcg tcggccagcc cagcacgagc
     3901 taccgcgagc gttccgccgg cgatttctcg cccaagctgg cgaccctgtt caaggtcaac
     3961 gatgccttca acctgcgcgc cagcgccggt cgcgccttca acctgccgga catgttcaac
     4021 ctctacgcca atacccggcg cggcacgacg acctactggg cgaaccccga tctccagccg
     4081 gaaaccgtca aggcctacga cctcggtgcc gactactatt tcggccggcg cggttacgtc
     4141 aaggcgacgc tctattacaa cgacgccaag gacttcatct actcggtccg gcgcgacgcg
     4201 accaacgtcg acaagatcaa cgtcggcgcc gtcaccacgc gcggcctgga actcgaagcc
     4261 gcctaccgcc cgagcgaggc gctggcgctg agcgccagct tcacctacaa ccgctcgcgg
     4321 atcgaggaga acgccagcaa cccggcgctc gaaggcaagt cgctgaccag cgtgccgcgc
     4381 cgccaggggc aagtgcgcgc cgattaccgc tggcccggcg gcgccaccct gtccgccgcc
     4441 gtcaaccggg tcggcaagcg ctacgccgac gacatcaaca gcaagtccta caaggcttac
     4501 acgacgctcg accggagcgc cagctacccg ctgggcaaga ctttcggcgc ccgcctgaat
     4561 gtcgtcaatg tcggcgacaa ggtgtacgac ggcatcggct acctggcgcc cgggcgcacg
     4621 gtgagcgccg gtctttacgc cagcttctga aagcgcgctg gcagcctgag ggcggatgcc
     4681 agcgcgctac ttcttgcgta tcatcccgaa gcttcgccat cgttcgtcgc ccaccttgcg
     4741 cccgtcgtcc cttcctcctt cctcgccagc ggctgcccgc tggccgggca tcgtcctcgt
     4801 cctggcggtg cacggcctgg ccttgtgggt cttgctggcg cagcggctgc tgccgctgcc
     4861 ggaggaagcg tcgacgctga tggtcaattt catcgccccg ccggcgccgc agcagacggc
     4921 acccaagccg gcgccgcgac cgcccgagcc gaagcccttg cccaagccgg cgccgcacca
     4981 actggtcgcc gaagcgccgg tgctcagtcc ggccgagccg gtggccccgc caccaccgcc
     5041 ggcgccgccg gtcgaggtgg cgccgccggc accgcccctg cccttgccgc agcgcccggt
     5101 ggccctgggc ggcgagttgt cggtggtttg cccggagcgg caggcgccgg cctatccgcc
     5161 gatgtcgcgc cggcttggcg agaccggcaa ggtggtgctg cgggtgacgc tcgacgagcg
     5221 cggcaacgtc gcccgggcga gcatcgaacg cagcagcggc catccgcggc tcgacgaggc
     5281 ggcgctcggc gccgtgcgcg gctggcgctg cacgccggcc cggcgcaacg gccagccggt
     5341 cgaggcgacc gccttgcaac cctttaattt cgtcctggaa ggaaactgat cgatgcaacc
     5401 cgcagttgtc gaaggcctgg gctttgccca ttttcttgcc caggcggatg gtgtcgggcg
     5461 cgtcgtgctc ggcctgctgc tgctgctgtc ggtggcgagc tggtacctga tcgtcaccaa
     5521 gtggctcgcc aactatctgg cgcagcgcca ggcgagcgcc ttcctcgccc gcttctggca
     5581 gggcggctcg cgcagcggcc tcggccagcg cctgcgcaac gagaagatcg acaacgcctt
     5641 cgcccgcctg gcgttgaatg ccatcgaggc ggccgaggaa gcggcggccg gcgacgacaa
     5701 cctggcgacc gccggcggtc tcgccgaata cctgacgcgg acgctgaaca acaccatcga
     5761 ccaggaagcg gcgctctccg aacacggcct caccgtgctc gcctcggccg gctcggcggc
     5821 gccctacatc ggcctcttcg gcaccgtctg gggcatctac cacgcgctgg tgcagatcgg
     5881 cctgtccggc caggggacgc tcgacaaggt cgccggaccg gtcggcgagg cgctgatcat
     5941 gaccgccctc ggtctcgccg tggcgattcc ggcggtgctc gcctacaacg ccttcagccg
     6001 gcgcaaccgg ctgtggctgg cgcgcctcga aggtttcgcg catgacctct acgtgctgct
     6061 gacggtgaag aacgaagccg ccggcggcgc ccggggcggc tgatggcggc cggcgacttc
     6121 cgccgctacc gcggccgggc cgacggcccg aaagccgaga tgaacgtcgt ccccctggtc
     6181 gacgtgatgc tggtgttgct ggtcatcttc atcgtcaccg cgccgctgct cacgcacgcg
     6241 gtcaagatcg acctgccgaa ggcgtcgagc gcggtgaacc tgaccaagcc cgagcacatc
     6301 gaattcggcc tgcgcgagag cggcgagatg ttctggaacg gcgagccggt gacgctcgaa
     6361 gaactgtcc
//
LOCUS       DAOSDX010000033         6322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4448_length_6322_cov_2.711824, whole genome shotgun sequence.
ACCESSION   DAOSDX010000033 DAOSDX010000000
VERSION     DAOSDX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_6322_cov_2.711824"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLN02_01305"
     CDS             complement(<1..385)
                     /locus_tag="PLN02_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOI50987.1"
                     /translation="MFNVVKKTFAYGEHQVTLETGEIARQAGGAVVVSMEETVVLVTV
                     VGAKNAKPGQDFFPLTVDYQEKVYAAGRIPGGFFKREGRPSEKETLTCRLIDRPIRPL
                     FPDGFYNEVQVVATVISLNPEIDSDI"
     gene            complement(523..792)
                     /gene="rpsO"
                     /locus_tag="PLN02_01310"
     CDS             complement(523..792)
                     /gene="rpsO"
                     /locus_tag="PLN02_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOI50988.1"
                     /translation="MAVTTEQKASIVAEYAQSKGDTGSPEVQVALLTARINDLQPHFK
                     EHSKDHHSRRGLLHLVSQRRKLLDYLKGKDVERYRTLITKLGLRK"
     gene            complement(899..1804)
                     /gene="truB"
                     /locus_tag="PLN02_01315"
     CDS             complement(899..1804)
                     /gene="truB"
                     /locus_tag="PLN02_01315"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOI50989.1"
                     /translation="MEPRVRKTWKQVDGVLLLDKAQGMTSNDALQKARRLFSAAKGGH
                     TGTLDPLATGLLPLCFGEATKFSADLLDADKTYEAELLLGVSTDSGDAEGVVTATAEV
                     NLTPADIARVLPQFTGDILQVPPMHSALKRDGRPLYELARQGITVDREARPVSIHAIE
                     LLEFAGSRLRLSVSCSKGTYIRVLAQDIGAALGCGAHLTALRRTRVGDLVIDGAMTLA
                     QIDSIEEGARVACLRPVDALLQSLPRIDLSGEAEQRFSHGNPVDLPEGLSGKIRVYAG
                     DRLIGVGEPGPGQRLWPKRLVQLAL"
     gene            complement(1812..2192)
                     /gene="rbfA"
                     /locus_tag="PLN02_01320"
     CDS             complement(1812..2192)
                     /gene="rbfA"
                     /locus_tag="PLN02_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOI50990.1"
                     /translation="MKKQGPRGFQRSDRVAEQVRRDLADLIRTELKDPRVGMISLTSV
                     ELTPDYAHAKVFFTTLNAEHLPEIEQGLKRAAGFLRRELGRRIHIHTLPELHFVYDSS
                     LEYGASMTALIQQANAISDQTPED"
     gene            complement(2192..4960)
                     /gene="infB"
                     /locus_tag="PLN02_01325"
     CDS             complement(2192..4960)
                     /gene="infB"
                     /locus_tag="PLN02_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOI50991.1"
                     /translation="MSATTVSQFAVELKMPVEALLEQLNRAGVGKSGGSDTLTDQDKA
                     GLLDYLRRAHGDAQPKNKITLTRKQTSEIKATDAHGRARTVQVEVRKKRVLVKRDVPD
                     ALAEGSTEGLEEQELVESAADLVQEPVVETLLEMVEPVAVEPEPEPEIEPEPQPEPEP
                     EPVPEPAAPVEAEPVAEAVVEAAPSETLAVRRPLDRAAIIGEKELKAREEEARRFSRL
                     REIQERELREKQAREAQLVQMRQQAEAAAAAAKQAEQAKQQAAAQAKTAEGEKGTLHK
                     RPDAPKKGEKGGRAADDGKKKGGLKTRGSDVGGGWKDNRHGHKKSHKSDDGQGSFQAP
                     TEPITREVHVPETISVSDLAHKMAVKATEVIKTMMKMGSMVTINQVLDQETAMIVVEE
                     MGHKALAAKLDDPDAFLEETGEHKDVPLEPRAPVVTVMGHVDHGKTSLLDYIRRAKVA
                     AGEAGGITQHIGAYHVETDRGMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGV
                     MPQTKEAIAHAKAAGVPLVVAVNKIDKPDANPDRVKQELVAEGVIPEEYGGDSPFCPV
                     SAKKGTGIDELLEQVLLQAEVLELTAQKDAPAKGLIIEARLDKGRGAVATMLVQSGTL
                     KRGDVVLAGQVFGKVRAMLDENGKPINEAGPSIPVEILGLSDVPAAGEEAIVLADEKK
                     AREIALFRQGKFRDVKLAKQQAAKLENMFQQMEEGQVKTLPLIVKADVQGSQEALVQT
                     LSKLSNEEVRVQIIHGAVGAISESDVNLAQASGAVIIGFNIRADAGSRKLAESFGVDI
                     RYYNVIYDAVDEVKAALSGMLAPEKREQVIGMVEIRQVFHVSKVGAIAGCYVLEGIVK
                     RNSRVRLIRNHTVQWDGELDSLKRFKDDVKEVRSNFECGLSLRGNNDIQEGDQLEVYE
                     IQEVARSL"
     gene            complement(4978..>6322)
                     /gene="nusA"
                     /locus_tag="PLN02_01330"
     CDS             complement(4978..>6322)
                     /gene="nusA"
                     /locus_tag="PLN02_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOI50992.1"
                     /translation="EVRVAIDRATGNYDSFRRWQVVADDEYVNEYLQIPLSDARKDDA
                     DVEAGDYLEEPLEPIDFGRIGAQAAKQVILQKIRDAEREQILQDFLERKEHVVSGTIK
                     RMERGNAIVEAGKIEAMLPRDQMIPKENLRVGDRVRAYLLRIDRGARGPQIILSRTAP
                     EFVIKLFELEVPEIEDGLMELKACARDPGMRAKIAVKSNDPRIDPIGTCVGLRGSRVT
                     AVRNEIGGENIDIVLWSADPAQFVIGALSPAEVTSIVVDEEKHAMDVVVDEDNLAIAI
                     GRSGQNVRLASELTGWTINLMTQDESAKRAEAEYAVTRVMFMEKLDIDEELADLLIEE
                     GFSTLEEVAYVPLAEMLEIDGLDEDIVNELRNRARNVLLTEAIATEEKLESVAEDLIG
                     LEGMTKELAAKLAEHNIKTRDDLAELAVDELLEIAGIDEARAKELILKARAHWFE"
BASE COUNT         1051 a         2033 c         1923 g         1315 t
ORIGIN      
        1 ggatgtcgga atcgatttcc ggattcagcg agatgacggt ggcgacgacc tggacttcat
       61 tgtagaaacc atccgggaac agcggacgga tcgggcggtc gatcagacgg caggtcagcg
      121 tttccttttc cgaaggacgg ccttcgcgct tgaagaagcc accggggatg cggccggcag
      181 cgtaaacttt ttcctgataa tcgacggtca gcgggaagaa atcctgaccg ggcttggcat
      241 tcttggcacc gacgacggtc accagaacga ccgtctcttc catcgagacg acaacggcac
      301 cgccggcctg gcgggcgatt tcgccggttt ccagggtgac ctggtgttcg ccgtaggcga
      361 aggtcttttt cacgacatta aacattgatt tcctttctct ctcatcacat taacgcaaca
      421 ataacaacaa cttacacaac aacaagaaac aacgggacaa caagaaaaca aaaaagcggc
      481 cgggaatttt ccgggccgct tttcgtggct acgccgtgct gcttatttac gcaggcccag
      541 cttcgtgatc agcgtgcgat aacgctcgac atccttgccc ttcaggtagt ccagcagctt
      601 gcggcgctgc gagacgaggt gcagcaggcc acgacgcgag tggtgatcct tgctgtgctc
      661 cttgaagtgc ggctggaggt cgttgatgcg ggcggtcagc agggcgacct gcacttccgg
      721 ggaaccggtg tcgcccttgg actgtgcgta ttcggcgacg atgctcgcct tttgttcggt
      781 ggtaacggcc atttcaaaac cctctttcat gaaaacgacg cagccccggt ttgatagagc
      841 gaacgccaat tggaaaattt tttcccgaca atatcggaaa gcggcggatt gtaccagctt
      901 aaagcgccag ttgcaccagt cttttcggcc acaggcgctg gcccggcccg ggctcgccga
      961 cgccgatcag gcggtctccg gcatagaccc ggatcttgcc gctcaagcct tccggcaaat
     1021 cgaccggatt accgtggctg aagcgctgtt cggcttcacc gctaagatca atccggggca
     1081 ggctctgcag cagcgcatcg accggacgca ggcaagcaac acgcgcgccc tcctcgatgc
     1141 tgtcgatctg cgccagcgtc atggcgccat cgatcaccag atcgccgacc cgggtccgcc
     1201 gcaaggcagt cagatgcgcg ccgcagccca gcgcggcgcc gatgtcctgg gccagcacgc
     1261 ggatataggt gcctttgctg caactgaccg acaggcgcag acggctgccg gcaaattcca
     1321 gcagctcgat ggcatgaatg ctcaccggcc gggcctcgcg gtcgacggtg atgccctgcc
     1381 gggcgagttc gtagagcggc cggccgtcgc gcttcagcgc tgaatgcatg ggcggcacct
     1441 gcaggatgtc gccggtgaat tgcggcagca cgcgggcgat gtcggctggc gtgagattga
     1501 cctcggcagt cgccgtgacg acgccctcgg cgtcaccgga atcggtgctg actccgagca
     1561 ggagctcggc ttcgtaggtc ttgtcggcgt cgagcagatc agcggaaaac ttggtcgcct
     1621 cgccaaaaca aaggggcagc aggccggtgg ccagcgggtc gagggtaccg gtgtgaccgc
     1681 ccttggcggc cgaaaacagg cgccgtgcct tctgcagcgc gtcgttcgaa gtcatgccct
     1741 gcgccttgtc gagcagcagc acgccgtcca cctgtttcca ggttttcctg accctcggct
     1801 ccatggcggg cctagtcttc cggcgtctgg tcgctgatcg cgttggcctg ctggatcagc
     1861 gccgtcatgc tggcgccgta ttccagggaa ctgtcgtaga cgaaatgcag ttctggcagg
     1921 gtgtgaatat ggatgcgccg ccccagctca cgccgcagga aaccggcggc acgcttcaga
     1981 ccctgctcga tctcgggcag gtgctcggcg ttgagcgtgg tgaagaaaac cttggcgtgc
     2041 gcgtaatccg gcgtcagttc gaccgaagtc aggctgatca tgccgacgcg cggatccttc
     2101 agttcggtac ggatcaggtc ggcgaggtcg cggcgcacct gctccgcgac ccgatcgcta
     2161 cgctggaatc ctcggggtcc ctgcttcttc attacagcga acgcgccact tcctggattt
     2221 cgtacacttc gagctgatcg ccttcctgga tgtcgttatt gccacgcagc gacagaccgc
     2281 attcgaagtt ggaacgcact tccttgacgt catccttgaa gcgcttcagc gaatccagct
     2341 cgccatccca ctgcaccgtg tggttgcgga tcaggcggac gcgggaattg cgcttgacga
     2401 taccttccag gacataacaa ccggcgatcg cgccaacctt cgaaacgtgg aagacctgac
     2461 ggatctcgac catgccgatc acctgctcgc gcttttccgg cgccagcatg cccgacaagg
     2521 ccgccttgac ttcatcgacc gcgtcgtaga tcacgttgta gtaacggata tcgacgccga
     2581 agctctcggc cagcttgcgc gaaccggcat cggcgcggat gttgaagccg atgatgacgg
     2641 cgcccgaggc ttgtgccagg ttgacgtcgg attcgctgat cgcaccgacc gcgccgtgga
     2701 tgatctggac gcggacttcc tcgttggaga gcttggacag ggtctgcacc agggcttcct
     2761 gcgaaccttg cacgtcggcc ttgacgatca gcggcagggt cttgacctgg ccttcttcca
     2821 tctgctggaa catgttttcc agcttggcgg cctgttgctt ggccagcttg acgtcgcgga
     2881 acttgccctg acggaacaag gcgatttcgc gcgccttctt ttcgtcggcc aggacaatgg
     2941 cttcctcgcc ggccgccggc acatccgaca gaccgaggat ttcaaccgga atcgacggcc
     3001 cggcttcgtt gatcggcttg ccgttctcgt cgagcatggc gcgcaccttg ccgaagacct
     3061 gaccggcgag cacgacgtcg ccgcgcttca gggttccgga ctggacgagc atggtcgcca
     3121 ccgcaccgcg acccttgtcg aggcgggctt cgatgatcag gcccttggcc ggcgcatcct
     3181 tctgcgccgt cagttcgagc acttcggcct gcagcaggac ttgttcgagc agttcgtcga
     3241 taccggtgcc cttcttggcg gacaccgggc agaacggcga atcgccgccg tattcttccg
     3301 gaatcacgcc ttcggcaacc agttcctgct tgacgcgatc cgggttggca tccggcttgt
     3361 cgatcttgtt caccgccacc accagcggca caccggccgc cttggcgtgg gcgatcgctt
     3421 ccttggtctg cggcatgacg ccgtcgtcgg ctgccactac caggatgacg atgtcggtgg
     3481 ccttggcacc acgcgcccgc atcgccgtaa aggcttcgtg acccggggtg tcgaggaagg
     3541 tgatcatgcc gcgatcggtt tcgacgtgat aggcgccgat gtgctgcgtg atgccgccag
     3601 cttcgcccgc ggcgaccttg gcgcggcgga tgtaatcgag cagcgaggtc ttgccatggt
     3661 cgacgtggcc catgacggta accaccggag cacgcggctc cagcggcacg tccttgtgct
     3721 cgccggtttc ctcgaggaag gcatcgggat cgtcgagctt ggcggccagc gccttgtgac
     3781 ccatttcctc gacgacgatc atcgccgttt cctggtcgag gacctggttg atggtgacca
     3841 tcgagcccat cttcatcatc gtcttgatga cttcggtcgc cttcaccgcc atcttgtgcg
     3901 ccaggtcgga aaccgagatg gtttccggca cgtgcacttc gcgggtgatc ggctcggtcg
     3961 gcgcctggaa gctaccctgg ccatcgtccg acttgtgcga tttcttgtgg ccgtggcgat
     4021 tgtccttcca gccaccgcca acgtccgaac cgcgggtctt cagaccgccc tttttcttgc
     4081 catcgtcggc tgcacgaccg cccttttcgc ccttcttcgg cgcatccgga cgcttgtgca
     4141 gggtgccctt ttcgccttcg gcggtcttcg cctgggcagc cgcctgctgt ttcgcctgtt
     4201 cagcctgctt ggcagcggcg gcagcggctt ccgcctgctg gcgcatctgc accagctggg
     4261 cttcgcgggc ctgtttttcg cgcagttcac gttcctggat ctcgcgcagg cggctgaagc
     4321 gacgcgcctc ttcctcgcgc gccttgagtt ccttttcacc aatgatcgcg gcccggtcca
     4381 gcggacgacg aacggccagc gtttcgctcg gcgcggcttc gacgacagcc tcggctaccg
     4441 gctcggcttc aaccggcgct gcgggttccg gcacaggctc cggctccggc tcgggttgcg
     4501 gctccggctc gatttccggt tccggttccg gttcaacagc gaccggctcg accatctcga
     4561 gcagcgtttc gacaacgggt tcctgcacca gatcggcagc cgattcgacc agctcctgct
     4621 cttcgaggcc ttcagtagag ccctccgcca gcgcatccgg aacatcgcgc ttgaccagca
     4681 cgcgcttctt gcgcacttcg acctgcaccg tgcgggcccg cccatgggca tcggtcgcct
     4741 tgatctcgga cgtctgcttg cgcgtcagcg tgatcttgtt cttcggctgc gcatcgccat
     4801 gcgcccgacg cagatagtcg agcaaaccgg ccttgtcctg atcggtcagt gtatcgctac
     4861 caccggactt gccgacaccg gcgcgattga gctgctcgag cagcgcctcg accggcatct
     4921 tgagttcaac ggcaaattgt gaaacggttg ttgcggacat acttgctacc tccccgctca
     4981 ctcgaaccag tgagcccgag ccttgagaat gagttccttg gcacgcgctt cgtcgatgcc
     5041 ggcgatttcc agcagttcat cgaccgccag ttcggcgagg tcgtcacgtg tcttgatgtt
     5101 gtgttcggcc agcttggccg ccagttcctt ggtcatgcct tcgagaccga tcaggtcctc
     5161 ggcgacgctt tcgagcttct cctcggtggc gatggcttcc gtcaggagca cattgcgcgc
     5221 ccggttgcgc aactcgttga cgatgtcttc gtccaggccg tcgatctcga gcatttcggc
     5281 gagcggcacg taggccactt cctccagcgt cgagaaacct tcctcgatca gcaggtcggc
     5341 cagttcttcg tcgatgtcca gcttttccat gaacatcacg cgggtcaccg cgtattcggc
     5401 ttcggcgcgc ttggccgact catcctgggt catcaggttg atcgtccagc cggtcagttc
     5461 cgaggccagg cgcacgttct gcccgctgcg gccgatggcg atcgccaggt tgtcctcgtc
     5521 gacgacgacg tccatggcat gcttttcttc atcgacgacg atcgaggtga cctcggccgg
     5581 cgacagcgcg ccgatcacga actgggccgg atcggccgac cacagcacga tgtcgatgtt
     5641 ctcgccgccg atctcgttgc gcacggcggt gacgcgcgaa ccgcgcagac cgacgcaggt
     5701 accgatcggg tcgatgcgcg ggtcgttcga cttgacggcg atcttggcgc gcatgccggg
     5761 atcgcgggcg caagccttca attccatcag accgtcctcg atctccggaa cttccagctc
     5821 gaacagcttg atgacgaatt ccggcgccgt gcgcgacagg atgatctgcg gaccgcgggc
     5881 accacggtcg atgcgcagca ggtaggcacg gacacggtcg ccgacacgca gattttcctt
     5941 cggaatcatc tggtcgcgcg gcagcatggc ttcgatcttg cctgcctcga cgatggcgtt
     6001 accacgttcc atgcgcttga tcgtgccgga aacgacgtgt tccttgcgct ccaggaagtc
     6061 ctgcaggatc tgctcgcgct cggcgtcgcg gatcttttgc aggatcacct gcttggcggc
     6121 ctgggcaccg atgcgaccga aatcgatcgg ctccagcggc tcctccagat aatcgccagc
     6181 ctcgacatcg gcgtcgtcct tgcgcgcatc cgacagcgga atctggaggt attcgttgac
     6241 gtattcgtcg tccgccacca cctgccagcg gcggaaggag tcgtaattgc cggtggcgcg
     6301 atcgatcgcg acacggactt ca
//
LOCUS       DAOSDX010000034         6205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4541_length_6205_cov_3.533659, whole genome shotgun sequence.
ACCESSION   DAOSDX010000034 DAOSDX010000000
VERSION     DAOSDX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4541_length_6205_cov_3.533659"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLN02_01335"
     CDS             complement(<1..495)
                     /locus_tag="PLN02_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI50993.1"
                     /translation="MNCDELIAIDFHTHAEVSCCQGHDPFGDEFDRAADKYFGSDRRP
                     TIAETVAYYRERKIGLVMFTVDAEAQIGRRRIPNEEIAEAARANADLMIAFASIDPHK
                     GVMGAREARRLIEEYGVRGFKFHPTVQGFQPYDRFAWPLYEVIAEYQLPAIFHTGHSG
                     IGSGM"
     gene            complement(492..1241)
                     /locus_tag="PLN02_01340"
     CDS             complement(492..1241)
                     /locus_tag="PLN02_01340"
                     /inference="COORDINATES: protein motif:HMM:NF006013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOI50994.1"
                     /translation="MLDVDFSDRLLRLTLARPAKRNAIDSALLGEIERAVDDLPESAG
                     AILIAGQGPHFCAGLDLAEHRARDAQAVFAISQRWQRLFSRLQESGLPIVVALSGAVI
                     GGGLELAALGHIRVADSTSFYQLPEGRHGVFVGGGGSVRIARLIGADRMGEMMLTGRR
                     LDAEEGARLGLSHYLVGPGEALARAEALARQVLENASLANWAIVSALPRIAELSAGNG
                     FFVESLTAALTQTGPEVAGRIGAFLDKGAVS"
     gene            complement(1249..3108)
                     /locus_tag="PLN02_01345"
     CDS             complement(1249..3108)
                     /locus_tag="PLN02_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HOI50995.1"
                     /translation="MNNPAFQSVIDSDFRPVSIGHPPPEIHHDADGVWHIRPLEALGD
                     YPPRLTDRLVDGARDFPERTLMAARGPDGEWIRIGYGEMLQRARRIGQWLLDHGVSVE
                     RPLLVLSGNDLEHIQLMLGAMYAGVPYSSVSPAYSLVATDFGKLRHIVGTVTPSVVYA
                     AAGEPFARAIAGAIDGDVMVVLGKGELSERASIPFAELLQAEPTTVDADNAAVGPDTI
                     AKFLFTSGSTKLPKAVTTTQRMLCANQQMLLQTFPVFAEEPPVLVDWLPWNHTFGGSH
                     NVGIALYNGGSFYIDDGQPTPKRFAETLRNLREISPTVYFNVPKAWEDLTVVLEHDAE
                     LREVFFAKLKLFFFAGAGLSQAAWDRLDRVTEAHCGHRIRMMVGLGMTETSPSCLFST
                     GGVIGAGYVGLPAPGCEVKLVPTDGKLEARMRGPHVMPGYWRNAEQTRAVFDADGYFC
                     TGDALKFVDEARPELGMVFDGRIAEDFKLSSGTFVSVGPLRAKVITEGAPYVQDIVVA
                     GMNRDEIGVLVFARVPECRQLAGLGDDADLFAVMTHPKVREHFAGLLTRLNRTATGSA
                     NRIAWLRLLETLPSIDHSEVTDKGSINQRAVLTRRAELIDALYAGQDPFLIAG"
     gene            complement(3147..4931)
                     /locus_tag="PLN02_01350"
     CDS             complement(3147..4931)
                     /locus_tag="PLN02_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOI50996.1"
                     /translation="MDSYAAPLRDMRFILDHLVGIDRIAALPGCADAEPDLVHSILEE
                     ADKFAAGVLAPLNKVGDREGCRLDGDRVVVPNGWQEAWDQFVASGWLGLSLPAEYGGQ
                     GLTKSLWAPVWEMWFSSNLAFTMLPQLTLGQTEALEVAADETLKARYLPKIVSGEWAC
                     NMSLTEPQAGSDLAAVRMRAEPAGDGSYRLSGQKIFISYGDHELAANIVHLVLARLPD
                     APPGIKGISLFLVPKFLVNADGSLGEKNDVRAVALEHKLGIHGSPTCTLAYGDHGGAV
                     GYLVGELNRGIEYMFVMMNDARFGVSVHGVALAERAYQMALRYASERVQGKVLGAAGL
                     PIAAHPDVKRQLLAMRTRIFAMRALVYTAAGWFDHARHNPDQEAAARSRRYIDLLMPV
                     LKAWNTETGNLVCDDAIQVFGGMGFVEETGITQHYRDSRVTRIYEGTTGIQANDLVGR
                     KILRENGATLRELLADIRAETTALALVNDLAGHAHALSVNLADLERSLDFLLAQGQGA
                     PALVQAAAVPFLHLSGLVCAGWQVARAARAAHAGLATGAEHADYLRGVLAIADFWFAT
                     YAPQVRAWSETVQAAACVGAAPDAALHG"
     gene            complement(5007..>6205)
                     /locus_tag="PLN02_01355"
     CDS             complement(5007..>6205)
                     /locus_tag="PLN02_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOI50997.1"
                     /translation="GVDEAAPTPIWYGFNAMLAANMLRDAAGLVTQVGGEVIPSDIDG
                     NLAMAVRQPAGVVLGIAPWNAPVILGTRAIAVPLACGNTVVLKSSELTPGLHRLIGEV
                     LLEAGIPAGVVNVISNAPADAPAIVERLIANPAVKRVNFTGSTHVGRIIGQLAAKHLK
                     PAVLELGGKNPMLVLDDADLDEAVTAAAFGAYFNQGQICMSTDRIIVSRSIAASFVER
                     LAAKLATMRAGKPGSEGCILGAMVTPEAAARVERLVADAVAKGASLPTGAVQCDGAVL
                     QPVLVDGLTPEMELYREEAFGPVVTVLRVDSDEEAIRLANDSEYGLSASVFSKDIGRA
                     LAVAKRIESGICHINSSTVHDEAQMPFGGVKASGYGRFGGKAGVAEFTDLRWITIQTT
                     HRHYPV"
BASE COUNT          996 a         2044 c         2196 g          969 t
ORIGIN      
        1 catgccgctg ccgatgccgg aatggccggt atggaagatc gccggcaatt ggtactcggc
       61 gatcacctcg tagagcggcc aggcgaaacg gtcatacggc tggaagccct gcaccgtcgg
      121 gtggaacttg aagccgcgca cgccgtattc ctcgatcagc cgccgcgcct cgcgggcacc
      181 catgacgccc ttgtgcggat cgatgctggc aaaggcgatc atcaggtcgg cattggctcg
      241 cgccgcctcg gcgatttcct cgttgggaat gcgccggcgg ccgatctgcg cctcggcatc
      301 gacggtgaac atgaccaggc cgatcttgcg ttcgcggtaa taggcgacgg tctcggcaat
      361 ggtcggccgg cgatccgagc cgaaatactt gtcggcggcg cggtcgaatt cgtcgccgaa
      421 ggggtcgtgg ccctggcagc acgagacttc ggcgtgcgta tggaaatcga tggcgatcag
      481 ctcgtcgcaa ttcatgacac agcccccttg tccaggaaag cgccgatccg cccggcgact
      541 tcaggcccgg tctgggtcag cgccgcggtc agcgattcga cgaagaagcc gttgccggcg
      601 ctcagttcgg caatgcgcgg caacgccgag acgatcgccc agttggccag gctggcgttt
      661 tccagcactt gccgcgccag tgcctcggcc cgggccaggg cttcgccggg gccgaccagg
      721 tagtgcgaga ggccgaggcg agcgccttcc tcggcatcga gccgacgacc ggtcagcatc
      781 atctcgccca tgcggtcggc gccgatcaga cgggcgatgc gcaccgagcc accaccgccg
      841 acgaacaccc cgtggcgccc ttccggcaac tggtagaagc tggtgctgtc ggcgacacga
      901 atatgaccga gcgccgccag ttcgagaccg ccgccgatca ccgcgccgga caacgcgacg
      961 acgatcggca agccgctttc ctggagccgg ctgaacagcc gctgccagcg ctgcgagatg
     1021 gcgaacaccg cctgcgcatc gcgcgcccgg tgttcggcga ggtcgagacc ggcgcagaaa
     1081 tgcggcccct ggccggcgat caggatcgcc ccggcgcttt ccggcaggtc gtcgaccgcc
     1141 cgctcgattt cgccgagcag cgcactgtcg atcgcgttgc gcttggccgg ccgcgccagt
     1201 gtcaggcgca gcaggcggtc gctgaagtcg acgtcgagca tgggcgtctc aaccggcgat
     1261 caggaaaggg tcctgaccgg cgtacaaggc gtcgatcagc tcggcgcggc gggtcagcac
     1321 cgcgcgctgg ttgatcgagc ccttgtcggt gacttcgctg tggtcgatcg acggcagggt
     1381 ttcgagcagg cgcagccagg cgatgcggtt ggccgagccg gtcgccgtgc ggttgaggcg
     1441 cgtcagcagg ccggcgaaat gctcgcgcac cttcgggtgg gtcatcaccg cgaagaggtc
     1501 ggcatcgtcg ccgaggccgg ccagttgccg acactcggga acccgcgcga agaccagcac
     1561 gccgatctcg tcgcggttca tgccggcgac gacgatgtcc tggacatagg gcgcgccctc
     1621 ggtgatcacc ttggcgcgca gcggcccgac gctgacgaag gtgccggagg agagcttgaa
     1681 gtcctcggcg atgcggccat cgaagaccat gcccagctcg ggccgggctt cgtcgacgaa
     1741 cttcagcgcg tcgccggtgc agaaatagcc gtcggcgtcg aacaccgccc gcgtctgctc
     1801 ggcattgcgc cagtagccgg gcatcacgtg cgggccgcgc atgcgcgcct cgagcttgcc
     1861 gtcggtcggc accagcttga cctcgcagcc gggcgccggc aggccgacgt agccggcgcc
     1921 gatgacgccg ccggtggaga acaggcagga cggcgaagtc tcggtcatgc cgaggccgac
     1981 catcatccgg atacggtgcc cgcaatgcgc ttcggtgacg cggtcgaggc ggtcccaggc
     2041 ggcctgcgag aggccggcgc cggcgaagaa gaagagcttg agcttggcga agaagacctc
     2101 gcgcaactcg gcgtcgtgct cgaggacgac ggtcaggtct tcccaggcct tgggcacgtt
     2161 gaaatagacg gtcggcgaaa tctcgcgcag gttgcgcagg gtttcggcga agcgcttggg
     2221 cgtcggctgg ccgtcgtcga tgtagaagct gccgccgttg tacaaggcga tgccgacgtt
     2281 gtggctgccg ccgaaagtgt ggttccaggg cagccagtcg accagcaccg gcggttcttc
     2341 ggcgaacacc gggaaggtct gcagcagcat ctgctgattg gcgcagagca tgcgctgggt
     2401 cgtcgtcacc gctttcggca gcttggtcga gccggaggtg aacaggaact tggcgatggt
     2461 gtcggggcca accgcggcgt tgtcggcatc gaccgtggtc ggctcggcct gcagcagttc
     2521 ggcgaaggga atgctggcgc gctcggacaa ctcgcctttg ccgagaacga ccatgacgtc
     2581 gccgtcgatc gcgccggcga tcgcccgcgc gaagggctcg ccggcggcgg catagacgac
     2641 gctcggggtg accgtgccga cgatgtggcg cagcttgccg aagtcggtgg cgaccagcga
     2701 ataggccggc gacaccgacg aatacggaac gccggcgtac atcgcgccga gcatcagctg
     2761 gatgtgctcg aggtcgttgc cggacagcac cagcagcggc cgttcgaccg agacgccgtg
     2821 gtcgagcagc cactggccga tacggcgcgc acgctggagc atctcgccgt agccgatgcg
     2881 gatccattcg ccgtccggac cgcgggccgc catcagcgtg cgctccggga aatcgcgcgc
     2941 gccgtcgacc aggcggtcgg tcaggcgcgg cgggtaatcg ccgagcgctt cgagcggccg
     3001 gatatgccag acgccgtcgg cgtcgtggtg gatttcgggg ggcggatggc cgatgctgac
     3061 cgggcggaaa tcactgtcga tgacgctttg aaatgctggg ttgttcatgt tgtctcctca
     3121 atttttttct gtgggtctgg cgccggctag ccgtgcagcg ccgcatccgg cgccgcgccg
     3181 acgcaggccg cggcctgcac cgtctcgctc caggcacgga cctgcggcgc gtaggtggcg
     3241 aaccagaaat cggcgatggc gagcacgccg cgcaggtagt cggcgtgctc ggcaccggtg
     3301 gccagcccag cgtgcgcggc gcgtgccgcc cgagccacct gccagccggc acagaccagg
     3361 ccgctcaggt gcaggaaggg cacggcggcg gcctggacca gcgccggcgc cccctggccc
     3421 tgcgcgagca ggaagtcgag cgagcgttcg aggtcggcaa gattgaccga caaggcatgg
     3481 gcgtggccgg ccaggtcgtt caccagcgcc agggcggtgg tctcggcgcg gatgtcggca
     3541 agcagttcgc gcagcgtggc gccgttctcg cgcaaaatct tgcggccgac caggtcgttg
     3601 gcctggatgc cggtcgttcc ctcgtagatc cgggtcaccc gcgaatcgcg gtaatgctgg
     3661 gtgatgccgg tttcctcgac gaagcccata ccgccgaaga cctggatcgc gtcgtcgcag
     3721 accaggttgc cggtttcggt gttccaggcc ttgagcaccg gcatcagcag gtcgatgtag
     3781 cggcggctgc gggcggcggc ttcctggtcg ggattgtgcc gggcgtggtc gaaccagccg
     3841 gcggcggtgt agaccaaggc ccgcatggcg aaaatgcgcg tacgcatcgc cagcaactgg
     3901 cgcttgacgt cgggatgcgc ggcgatcggc aggccggccg cccccagcac cttgccctgg
     3961 acgcgctcgc tggcatagcg cagcgccatc tggtaggcgc gctcggcgag cgcgacgcca
     4021 tggacgctga cgccgaaacg ggcgtcgttc atcatcacga acatgtattc gatgccgcgg
     4081 ttcagttcgc cgaccagata gccgacggcg ccgccatggt cgccgtaggc cagcgtgcag
     4141 gtcggactgc cgtggatacc aagcttgtgt tcgagggcga cggcgcgcac gtcgttcttc
     4201 tcgcccaggc tgccatcggc gttgaccagg aacttcggca cgaggaacag cgagatgccc
     4261 ttgatgccgg gcggcgcgtc gggcagccgg gcgagcacca gatggacgat gttggcggcc
     4321 agttcatggt cgccgtagga aatgaagatc ttctggccag acagccggta actgccgtcg
     4381 ccggccggtt cggcacgcat gcgcacggcg gcgaggtcgg aaccggcctg cggctcggtc
     4441 aggctcatgt tgcaggccca ttcgccggaa acgatcttcg gcagataacg cgccttgagc
     4501 gtttcgtcgg ccgccacttc gagcgcctcg gtctggccga gggtgagctg cggcagcatg
     4561 gtgaaggcga ggttggacga gaaccacatt tcccagaccg gcgcccacag cgacttggtc
     4621 agcccctgac cgccgtactc ggcgggcaag gacaggccga gccagccgct ggcgacgaac
     4681 tggtcccagg cttcctgcca accattcggc acgacgaccc ggtcgccgtc gaggcggcag
     4741 ccttcacggt cgccgacctt gttgagcggc gccagcacgc cggccgcgaa cttgtcggct
     4801 tcctcgagga tgctgtgcac gaggtccggt tcggcgtcgg cgcaacccgg cagggcggcg
     4861 atccggtcga tgccgaccag atggtcgagg atgaagcgca tgtcgcgcag gggcgcagcg
     4921 taactgtcca tacgaatttc cagaattgat cgtttgacgc ggcggagcgg cccggcgacg
     4981 gaccgccccg cccttgaggc agtgccttac accgggtagt ggcgatgcgt ggtctggatg
     5041 gtgatccagc gcaggtcggt gaattcggca acgccggcct tgccgccgaa gcggccgtaa
     5101 ccggacgcct tgacgccacc gaaaggcatc tgcgcctcgt catgcacggt cgacgagttg
     5161 atgtggcaga tccccgactc gatgcgcttg gccaccgcca gcgcccgccc gatgtccttc
     5221 gagaacaccg acgccgacaa gccgtactcg ctgtcgttgg ccaggcggat cgcctcttcg
     5281 tcgctatcga cgcgcagcac cgtgaccacc ggaccgaaag cctcttcccg gtacagctcc
     5341 atttccggcg tcagaccgtc gaccagcacc ggctgcagca cggcgccgtc gcactggacc
     5401 gccccggtgg gcaggctggc gcccttggcg acggcgtcgg cgaccaggcg ctcgacgcgc
     5461 gccgccgctt ccggcgtaac catggcgccg aggatgcagc cttcgctgcc cggcttgccg
     5521 gcccgcatcg tcgccagctt ggctgccagc cgctcgacga agctcgcggc aatgctgcgc
     5581 gagacgatga tgcggtcggt cgacatgcag atctggccct gattgaagta ggcgccgaag
     5641 gccgccgccg tcaccgcttc gtcgagatcg gcgtcgtcga gcaccagcat cgggttcttg
     5701 ccgcccagtt cgagcaccgc cggcttcagg tgcttggcgg cgagctggcc gatgatccgg
     5761 ccgacgtggg tcgagccggt gaaattcacc cgcttgaccg ccgggttggc gatcagccgc
     5821 tcgacgatgg ccggcgcgtc ggccggcgcg ttggagatga cgttgacgac gccggccgga
     5881 atgccggctt cgagcaggac ctcgccgatc aaccggtgca ggcccggggt cagctccgag
     5941 gacttgagga cgacggtgtt gccgcaagcc agcggcacgg cgatcgcccg cgtgccaaga
     6001 atcaccggcg cgttccacgg cgcgatgccg agcacgacac cggccggctg gcgcaccgcc
     6061 atcgccaggt tgccgtcgat gtcggagggg atgacctcgc cgccgacctg ggtgaccaga
     6121 ccggccgcgt cgcgcagcat gttggccgcc agcatggcgt tgaagccgta ccagatcggc
     6181 gtcggcgcgg cttcgtcaac gccgg
//
LOCUS       DAOSDX010000035         6191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4556_length_6191_cov_3.935626, whole genome shotgun sequence.
ACCESSION   DAOSDX010000035 DAOSDX010000000
VERSION     DAOSDX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4556_length_6191_cov_3.935626"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLN02_01360"
     CDS             <1..349
                     /locus_tag="PLN02_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOI50998.1"
                     /translation="ALLWAFGTLIGNTDMHAGNLSFVSRHGRPYQLAPAYDVLPMSFA
                     PRAGGALVDTLAPAALIATVDADTWRQALQLAEHFFARLSDTAAFSPRFQPCIAALDR
                     HIDEAARRIARLG"
     gene            complement(401..1267)
                     /locus_tag="PLN02_01365"
     CDS             complement(401..1267)
                     /locus_tag="PLN02_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI50999.1"
                     /translation="MSELRAKAYAARLGKVLDYIEAHLDENLSLEQLSQVANFSKFHF
                     HRQFADFVGASVGRYVLLLRLRRASYQLAFNRKVKVIDIALEAGFENPESFSRAFRNV
                     FGKSPSAFRKAPDWVSWHERYRFRIPERTRNMQVEIVNFIPTSVAVYEHRGPAERVNE
                     SVGQFIAWRQQSGLSPKERSRTFGIAYDNPDTTPPEQFRFDIAGEVQAEVLANAQGVV
                     TRQIPGGRCARVRHVGSHTRIGESIYPLYREWLPASGEELRDFPLYFHYLNLLPETAE
                     ADLLTDIYLPLK"
     gene            1462..3243
                     /gene="cptA"
                     /locus_tag="PLN02_01370"
     CDS             1462..3243
                     /gene="cptA"
                     /locus_tag="PLN02_01370"
                     /inference="COORDINATES: protein motif:HMM:NF007933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HOI51000.1"
                     /translation="MLPQTLGRADCDQIELESRPSKRTKHSSMRSIDWRGLARAFVFF
                     WYFSAVTHALLFFSGATGFLPLRQGFVFSLLWLVPLLLFPNRARPISAAIGVVLGLSA
                     LINLGYFSIYGQEFSQSVLFIVFESNPAEASEYVDQYLKWWMFPGLIAFCAGAYGLWR
                     GIRPLAMAPAGRYLTIAVILAGLFLQPTYKVLRKRGLNPAKYVEAIGNRMEPAVPWQF
                     VIGYARYREQLAGMEALRKNTERIPPIENLVDQRAGQPGTIVLLIGESTTRQHMSLYG
                     YGRPTNPKLEAMRDQLTIFDQVYATRPYTIEALQQILTFADQQNPDLYLTRPSLMSIM
                     KQAGYRTYWITNQQTLTKRNTMLTNFSEQMDEQVYLNHQRSQNSYSFDSNVLEPLQNI
                     LKDGAERRFIVVHLLGTHMRYKYRFPPEFAVFRDGSGLPEWASGERAEMIDEYDNAVR
                     YNDHVVSSVIGSLAAADKASLLVYFSDHGEDVYDTPPHDFIGRNEAKPTPPMYTVPFF
                     LWTSPQWRAATPVDLAAARSRPYQLSHFIHTWADLTGLRFAGYEPDKSLINPAFRERE
                     ILVGNPADQKSLLDLRQLLKPPAATRP"
     gene            3251..3907
                     /locus_tag="PLN02_01375"
     CDS             3251..3907
                     /locus_tag="PLN02_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOI51001.1"
                     /translation="MPRYPHILFDLDGTLIDSAPAILASFRDAFAQTGIAPARTIDDS
                     VIGPPLNETLQLLSGSADAALIARLAEAFKASYDSTGYQATAAYAGVGELLSQLAGAG
                     LTLSIATNKRLHPTRLILDHLGWGGYFAQVYALDLFTPRLPNKAAMIGRLLADQGIAR
                     EDVIYIGDRSEDGESADANGLPFVAVTWGYGSLNADEMRPGWQAAAQPAALAALLLAD
                     "
     gene            4117..5073
                     /locus_tag="PLN02_01380"
     CDS             4117..5073
                     /locus_tag="PLN02_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOI51002.1"
                     /translation="MKLRKQLRSLIVASTLAIGASAGQAAEFINVLTGGTSGVYYPLG
                     VSLTQIYGKVLPDAKMSVQATKASAENLNLLQAGKGEIAFTLGDTLSEAWKGNPEVGF
                     KAPLNKLRTVAAIYPNYIHFLASADSGIKSLADLKGKRVSVGAPKSGTELNSRDILKA
                     VGMGYQDFAKVEYLGYSESVELMKNRQLDATLLSSGLGVAAVRDLATALKIVVIPIPA
                     DVVAKIGDAAYQSGTIPANTYNGQTTDVNTVLVPNYLVTHAGVSEDMVYKMTKSMAEN
                     LDTMATAHAAAKAIKKERMASGSPVPLHPGAEKYYREAGLLK"
     gene            5080..>6191
                     /locus_tag="PLN02_01385"
     CDS             5080..>6191
                     /locus_tag="PLN02_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOI51003.1"
                     /translation="MSAHVSPPNIEQETFSEHGAQRRLGGVALLVLTGTALAFSLYQL
                     GIAAFHPLSSLVMRALHVGFLLTLTFLLFPIRKSGRLDRVAPLDWLLASGAFALACYQ
                     WIFEVDLIQRAGDPSTLDLIVGTAMIALVFEAARRILGLALPTICALFLLYGLFGQHL
                     PGDLAHRGYDFAQIVDQLGFGTEGIFGIPTLVSTNYIFLFVLFGAFLEHAGMINLFNS
                     VALGFVGHAKGGAAKVAVVSSGLMGSISGSGVANVLTTGQFTIPLMKRFGYSGVFAGA
                     VEATASMGGQIMPPVMGAVAFIMAENLNVPYVEIVKAALIPAVLYYLTVFWMVHLEAG
                     RRKLGGLAKEECPNPWQAIRSQWYLVLPLVGLVGML"
BASE COUNT         1077 a         2231 c         1812 g         1071 t
ORIGIN      
        1 cgccttgctc tgggccttcg gcacgctgat cggcaatacc gacatgcacg ccggcaacct
       61 gtccttcgtc agccgccacg gccgtcccta ccagctggcg ccggcctacg atgtgctgcc
      121 catgagcttc gccccgcgcg ccggtggcgc gctcgtcgat acactggcac cggctgccct
      181 gatcgccacc gtcgatgccg atacctggcg gcaggcgctg caactggccg agcatttctt
      241 cgcccggctc agcgacaccg ccgccttctc gccgcgcttc cagccctgca tcgcggcgct
      301 ggaccggcat atcgacgaag cggcacggcg catcgcccgg ctggggtaag ccgtgccgct
      361 gcccggcccc cccccggcgg ctaccgggca gtgcaatcgg ctatttcaac ggcagataga
      421 tgtccgtcag cagatcggct tccgccgttt ccggcagcag attcagataa tgaaaataca
      481 gcggaaagtc gcgcagttcc tcgccgcttg ccggtagcca ctcgcgatac agcgggtaga
      541 tgctttcgcc gatgcgggtg tgcgagccga cgtgccgcac ccgtgcgcag cggccgccgg
      601 gaatctgcct ggtgacgacg ccctgcgcat tggccagcac ctcggcctgc acttcgccgg
      661 cgatgtcgaa gcggaattgt tccggcggcg tcgtgtcggg gttgtcgtag gcgatgccga
      721 aggtgcggct gcgctccttg ggcgacagcc cgctttgctg ccgccaggcg atgaactggc
      781 cgaccgactc attgacgcgt tcggccgggc cgcggtgctc atacacggca accgacgtgg
      841 ggatgaaatt gacgatttcg acctgcatgt ttctggtcct ttccgggata cggaatcgat
      901 aacgctcgtg ccacgagacc caatccggcg cttttcggaa tgcggaaggc gatttgccga
      961 agacattccg gaaggcgcgg gaaaacgatt ccgggttttc aaaaccggcc tccagcgcga
     1021 tgtcgatcac cttcaccttg cgattgaagg ccagttggta gctggcccgc cgcaaacgca
     1081 gcaggagaac gtaacgaccg acgctggcgc cgacaaagtc ggcgaattgc cggtggaaat
     1141 ggaatttgga aaagttggcg acctggctca gttgctccag gctcaggttt tcatcgaggt
     1201 gggcctcgat gtaatcgagc actttgccca atcgggccgc ataggccttc gccctgagtt
     1261 cactcatcgt tcttcctcta tccacggcgc cactatgcgt gaagtcgagg cggaaatcct
     1321 gacgccgatt gctctaaacc cgcaccgccc tcccccttgc cgcgtgcgcc ttgcggtagc
     1381 tgtcgagcgg gggctggtgg cgcttcagac cttccactgg ctgatccgga tcaggcctcg
     1441 cggaaagcca acgccttgtt ggtgttgccc caaaccctgg gccgggcgga ttgcgaccaa
     1501 atcgaactag aatcacgtcc ttcgaaaaga acaaagcact cgtccatgcg ctcaatagat
     1561 tggcgcggcc tcgcccgcgc ctttgtcttt ttctggtatt tctcggccgt cacccacgcg
     1621 ctgctcttct tcagcggtgc caccggcttc ctcccgctgc gccagggctt cgtcttcagc
     1681 ctgctctggc tggtcccgct gctcctcttc cccaaccgcg cgcggccgat cagcgccgcc
     1741 atcggcgtcg tcctggggct gagcgcgctg atcaacctcg gctatttctc gatctacggc
     1801 caggagttct cgcagagcgt cctcttcatc gtcttcgaat ccaatccggc ggaagccagc
     1861 gaatacgtcg accagtacct gaagtggtgg atgttccccg ggttgatcgc cttctgcgcc
     1921 ggcgcctacg gcctctggcg cggcatccgg ccgctggcga tggcgccggc cggacgctac
     1981 ctgacgatcg ccgtgatcct cgccggcctg tttctacagc cgacgtacaa ggtgttgcgc
     2041 aaacgtggcc tgaacccggc gaaatatgtg gaagcgatcg gcaaccgcat ggaaccggcg
     2101 gtgccctggc agttcgtcat cggctacgcg cgttaccgcg agcaactggc cggcatggag
     2161 gcattgcgca aaaacaccga gcgcatcccg ccgatcgaaa atctcgtcga ccagcgcgcc
     2221 ggccagccgg gaaccatcgt gctgctgatc ggcgaatcga ccacccgcca gcacatgagc
     2281 ctctacggct acggccggcc gacgaacccg aaactggaag caatgcgcga ccagctgacc
     2341 attttcgacc aggtctatgc cacgcgcccc tacaccatcg aagcgctgca gcagatcctg
     2401 accttcgccg accagcagaa cccggacctc tacctgaccc ggccgtcgct gatgagcatc
     2461 atgaagcagg ccggctaccg cacctactgg atcaccaacc agcagacgct gaccaagcgc
     2521 aacacgatgc tgaccaactt ctccgagcag atggacgaac aggtctacct caaccaccag
     2581 cgctcgcaga attcctacag cttcgacagc aacgtcctcg aaccgctgca gaacatcctc
     2641 aaggacggcg ccgagcggcg cttcatcgtc gtccacctgc tcggcacgca catgcgctac
     2701 aagtaccgct tcccgcccga gttcgccgtc ttccgcgacg gcagcggcct gcccgagtgg
     2761 gcaagcggcg agcgcgcgga aatgatcgac gagtacgaca acgcggtccg ctacaacgac
     2821 cacgtcgtct cgtcggtgat cggcagcctc gccgccgccg acaaggcgtc gctgctggtc
     2881 tatttttccg accacggcga ggacgtctac gacacgccgc cgcacgactt catcggccgc
     2941 aacgaggcaa aaccgacgcc accgatgtac accgttccct tcttcctatg gacttccccg
     3001 cagtggcgcg cagcgacgcc ggtcgacctg gcggccgccc gcagccgccc ctaccagctc
     3061 tcgcatttca tccacacctg ggccgacctc accgggctgc gctttgccgg ctacgagccg
     3121 gacaagagcc tgatcaaccc ggccttccgc gagcgggaaa tcctggtcgg caacccggcc
     3181 gaccagaaat cgctgctcga cctgcgccag ctattgaaac cgcccgccgc aacacgccct
     3241 tgagccccaa atgccccgct acccgcacat cctcttcgac ctcgacggca ccctgatcga
     3301 ttcggcgcca gcgatcctcg ccagcttccg cgacgccttc gcgcagaccg gcatcgcgcc
     3361 agcacggaca attgacgaca gcgtgatcgg cccgccgctc aacgagaccc tgcaactcct
     3421 ctcgggcagc gccgatgccg cgttgatcgc ccgcctcgcc gaggccttca aggcgagcta
     3481 cgacagcacc ggctaccagg cgaccgccgc ctacgccggc gtcggcgaac tgctttccca
     3541 actggccggc gccgggctga cgctgtcgat cgccaccaac aagcgcctcc acccgacccg
     3601 gctgatcctc gatcacctcg gctggggcgg gtatttcgcg caggtctacg cactcgacct
     3661 gttcaccccg cgcctgccca acaaggcggc gatgatcggc cgcctgctcg ccgaccaggg
     3721 catcgccagg gaagacgtca tctacatcgg cgaccgcagc gaggacggcg agtcggccga
     3781 cgccaacggc ttgcccttcg tcgccgtgac ctggggctac ggcagcctga acgccgacga
     3841 aatgcgaccg ggctggcagg cggcggcgca accggccgcc ctggcggcac tgctgctcgc
     3901 cgactgaaag cgcagcgccg acggtgcgga attccgcacc gggcgcggcg gcgattccgg
     3961 aattccgcac gcgcggccgt cgccggccac ccggcggccg ccgcttaacc ccgtgaaatc
     4021 agtcgcttgc ctgcccaagc caacaactgg cacagcttct gcaaacgttt ctctgcagcc
     4081 gcccagcggc gcattccaat ttacggagga gaaaccatga aactgcgcaa gcaactgcgt
     4141 tccctgatcg tcgccagcac gctggccatc ggcgccagcg ccggccaggc ggccgaattc
     4201 atcaacgtcc tgactggcgg cacgagcggc gtctattacc cgctcggcgt ctcgctgacc
     4261 cagatctacg gcaaggtgct gcccgatgcc aagatgtcgg tgcaggcgac caaggcctcg
     4321 gccgaaaacc tcaatctgct gcaggccggc aagggtgaaa tcgccttcac gctcggcgac
     4381 acgctgtccg aagcctggaa gggcaatccg gaagtcggct tcaaggcgcc gctgaacaag
     4441 ctgcgtacgg tcgccgcgat ctacccgaac tacatccact tcctcgcctc cgccgactcc
     4501 ggcatcaagt cgctggccga cctcaagggc aagcgcgttt cggtcggcgc cccgaagagc
     4561 ggcaccgaac tgaacagccg cgacatcctc aaggcggtcg gcatgggcta ccaggatttc
     4621 gccaaggtcg aataccttgg ttactcggag tcggtcgagc tgatgaagaa tcgccagctc
     4681 gacgccaccc tgctctcctc cggcctcggc gtggccgccg tgcgcgacct ggcgacggcg
     4741 ctgaagatcg tcgtcatccc gatcccggcc gatgtggtgg ccaagatcgg cgacgccgcc
     4801 taccagtccg gcaccatccc ggccaacacc tacaacggcc agaccaccga cgtgaatacc
     4861 gtgctcgtcc cgaactacct ggtcacccac gccggcgttt ccgaggacat ggtctacaag
     4921 atgaccaagt cgatggccga aaacctcgac accatggcca ccgcgcatgc cgccgccaag
     4981 gcgatcaaga aggaaaggat ggccagcggt tcgccggtgc cgctgcaccc gggcgccgag
     5041 aagtactacc gcgaagccgg cctgctcaag taagtcatca tgtcggccca cgtctccccg
     5101 ccgaacatcg agcaggagac gttctccgag cacggcgccc agcgccggct cgggggcgtc
     5161 gcgctccttg tcctgaccgg caccgcgctg gcgttttcgc tctaccagct cggcatcgcc
     5221 gccttccatc cactgtccag cctggtcatg cgggcgctgc acgtcggctt cctgctcacc
     5281 ctgaccttcc tgctcttccc gatccgcaag agcggccgcc tcgaccgcgt cgccccgctc
     5341 gactggctgc tcgccagcgg cgccttcgcg ctcgcctgct accaatggat cttcgaggtc
     5401 gatctgatcc agcgcgccgg cgacccgagc acgctcgacc tgatcgtcgg cacggcgatg
     5461 atcgccctcg tcttcgaggc tgcccgccgc atcctcggcc tggcgctgcc gaccatctgc
     5521 gcgctgttcc tgctctacgg cctgttcggc cagcacctgc cgggcgacct cgcccaccgc
     5581 ggctatgatt tcgcgcagat cgtcgaccag ctcggcttcg gcaccgaagg catcttcggc
     5641 atcccgaccc tggtgtcgac caactacatc ttcctcttcg tgctgttcgg cgcctttctc
     5701 gaacacgccg gcatgatcaa cctgttcaac tcggtggcgc tcggcttcgt cggccacgcc
     5761 aagggcggtg ccgccaaggt ggcggtggtt tcgtccgggc tgatggggtc gatttccggt
     5821 tccggcgtcg ccaacgtgct gaccaccggc cagttcacca tcccgctgat gaaacgcttc
     5881 ggctactccg gcgtctttgc cggcgcggtc gaggccaccg cctcgatggg cggccagatc
     5941 atgccgccgg tgatgggcgc cgtcgccttc atcatggccg agaacctcaa cgtaccctac
     6001 gtcgagatcg tcaaggcggc gctgattccg gccgtcctct actacctcac cgtgttctgg
     6061 atggttcacc tggaagccgg ccgacgcaag ctcggcgggc tggccaagga agaatgccct
     6121 aacccctggc aggcgatccg cagccagtgg tacctggtgt tgccgctggt cggcctggtc
     6181 ggcatgctgt t
//
LOCUS       DAOSDX010000036         6176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4576_length_6176_cov_3.430485, whole genome shotgun sequence.
ACCESSION   DAOSDX010000036 DAOSDX010000000
VERSION     DAOSDX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4576_length_6176_cov_3.430485"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLN02_01390"
     CDS             <1..456
                     /locus_tag="PLN02_01390"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOI51004.1"
                     /translation="GGQFSIEVAAYPEWHPQARSPKDDLQNFARKVKAGANSAITQYF
                     YNADAYFHFVDEARALGVDIPIIPGIMPIVGFTKLARFSDACGADLPRWMRKKFESYG
                     DDSESIRAFGLDLVTELCARLLQGGAPGLHFYSMNQAPLTLEICRRLGN"
     gene            complement(491..1312)
                     /locus_tag="PLN02_01395"
     CDS             complement(491..1312)
                     /locus_tag="PLN02_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOI51005.1"
                     /translation="MDAQSLSRYQDWLLAAATDVGLKVLAAIVFWVVGRWLIGIAVGM
                     VRAALERQKVDPTVLRYVGSIVTVTLNILLVIGILGYFGMQTTTFAALVAGAGIAIGA
                     AWAGLLSNFAAGAFLIVLRPFKVGDFVTVGGVTGTVTEIGLFATTLNTMDNIATVVGN
                     NKIFGDNIQNYTHNPFRRVDLKCQLAGAADHRAAIGLLREKIAMIPNVLAEPAVDVEI
                     LEFNLVGPVLAVRPYCHNDHYWQVYFETNRILREALAEAGFPAPMPAQNVLLTQS"
     gene            1500..1575
                     /locus_tag="PLN02_01400"
     tRNA            1500..1575
                     /locus_tag="PLN02_01400"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1533..1535,aa:Lys,seq:ttt)
     gene            1587..1662
                     /locus_tag="PLN02_01405"
     tRNA            1587..1662
                     /locus_tag="PLN02_01405"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1620..1622,aa:Lys,seq:ctt)
     gene            complement(1810..2250)
                     /locus_tag="PLN02_01410"
     CDS             complement(1810..2250)
                     /locus_tag="PLN02_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HOI51006.1"
                     /translation="MSCPLSIMTTADSATPEKTTDVRALLKELQERFAVFRNYSPLAI
                     GIDKQLFAALPDLSRKSVRLAMRSHTMSTRYLKEMEKAAQRLNLDGTPAGEVTDENRQ
                     HATEQLKERFKKQAEQRKASEAAAKAEAMKQKKLLELAEKFGRK"
     gene            complement(2299..3174)
                     /locus_tag="PLN02_01415"
     CDS             complement(2299..3174)
                     /locus_tag="PLN02_01415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOI51007.1"
                     /translation="MNDTAEFDSLVPAQGFSRRDFLVTSLGAGFALATQPVAAQTAIH
                     TDAAGLTAGEVKVPVHDGEMVAYRATPAGVGKAPVVLLISEIFGVHEYIRDTARRLAK
                     LGYFVVAPELFARQGDPRQIESVAEIIDKISSKTPDAEVMKDLDAAVVWAAGQGADTG
                     RLAITGFCWGGRITWLYSAHNPGVRAGVAWYGRLVSPVNAYNPRHPIDLVGELKAPVL
                     GLYGGVDTGIPVDTVDAMEAALKTGSPAAKASQLQLYDNAPHAFHADYRPSYRKEEAE
                     DGWRRMLDWFRRNGV"
     gene            complement(3193..4644)
                     /gene="rng"
                     /locus_tag="PLN02_01420"
     CDS             complement(3193..4644)
                     /gene="rng"
                     /locus_tag="PLN02_01420"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HOI51008.1"
                     /translation="MTEEILINFTPQETRVAVMQQGVVQELHIERTASRGLVGNVYLG
                     RIVRILPGMQSAFVDVGLERTAFLHVADIWQPRESATERPIEKILAEGQSIVVQVVKD
                     PIGTKGARLTTQLSIAGRMLVYLPQEKHIGISQRIESETERELLRERLTRLVPDDETG
                     GFIVRTMAENASDEEFATDIAYLKKICADIRQRSRSSAPPTLLYQELSLGQRVLRDFV
                     NPETTRIVIDSRENFQKLSLFAGEYTPAVQSLLEHYIGQRPLFDLHGVEDEIQKALAR
                     RVDLKSGGYLIIDQTEAMTTIDVNTGGFVGVRNFDDTIFKTNLEAAVTIARQLRLRNL
                     GGIIIVDFIDMENEEHKKSVLEEFNKALSRDHTRLTVNGFTHLGLVEMTRKRTRESLA
                     HVLCEPCPTCGGRGEVKTARTVAYEILRELLREARQFNAREYRILAGPAVVDLFLDEE
                     SQSLAMLSDFIGKAVSLQSEPSYSPEQYDIVLM"
     gene            4679..5953
                     /gene="yegQ"
                     /locus_tag="PLN02_01425"
     CDS             4679..5953
                     /gene="yegQ"
                     /locus_tag="PLN02_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HOI51009.1"
                     /translation="MRKAPELLAPAGSPEMMRTAFAFGADAIYAGQPRYSLRVRNNAF
                     GDLAILGDAIAEAHAAGKQFFVVSNIYPHNAKLKTYLDDMAPVIALQPDALIMSDPGL
                     IDMVRTRWPEQVIHLSVQSNTVNWAAVRFWQKMDISRVILSRELSLDEVAEIRQQCPD
                     IELEVFVHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVSTSDGQPVNFYQN
                     PEQEILLEERQRPGELMPIEEDEHGTYIMNSKDLRAIEHVHRLTEIGVDSLKIEGRTK
                     SPYYVARTCQAYRQAIDDAVAGRPFDPTLLGELENLANRGYTDGFYQRHHDADYQNYL
                     RGHSESDKSQYVGDAVAFDAARGLMRVAVKNRFALGDTVECVQPRGNLRSRIGRLENA
                     AGEPVPVAPGSGHTVWIDLPAETAGAFVSRLF"
BASE COUNT         1137 a         1996 c         1875 g         1168 t
ORIGIN      
        1 ggtggccagt tcagcatcga ggtcgccgcc tatccggaat ggcacccgca ggcgcgtagc
       61 cccaaggacg acctgcagaa tttcgcgcgc aaggtgaagg ccggcgccaa ttcggcgatc
      121 acccagtatt tctacaacgc cgacgcctac ttccactttg tcgacgaagc gcgcgcgctc
      181 ggcgtcgaca tcccgatcat cccgggcatc atgccgatcg tcggtttcac caagctggcc
      241 cgcttctccg acgcctgcgg cgccgatctg ccgcgctgga tgcgcaagaa gttcgagagc
      301 tacggcgacg atagcgaatc gatccgcgcc ttcggcctcg acctggtcac cgaactgtgc
      361 gcccgcctgc tccagggcgg cgcgccgggg ctgcacttct acagcatgaa ccaggcgccg
      421 ctgaccctgg aaatctgccg ccgcctcggc aactgagcca agggccggca acgccggccc
      481 cttccccgtc tcaggactgc gtcagcagca cgttctgcgc cggcatcggc gccgggaacc
      541 ccgcttcagc cagcgcttcg cgcagaatcc ggttggtctc gaaataaacc tgccaatagt
      601 ggtcgttatg gcaatacgga cgcaccgcga ggaccggccc caccaggttg aattcaagaa
      661 tctcgacatc aaccgccggc tcggcgagca cattcggaat catcgcgatc ttctcgcgca
      721 gcaacccaat cgccgcccgg tgatcggcag caccggctag ctgacacttc agatcgaccc
      781 ggcggaacgg gttgtgggtg tagttctgga tgttgtcgcc gaaaatcttg ttgttgccga
      841 ccacggtcgc gatgttgtcc atcgtgttca gcgtcgtcgc gaacaagcca atctcggtca
      901 ccgtcccggt gacaccgcca accgtcacga agtcaccaac cttgaagggg cgcagaacga
      961 tcaggaaggc ccccgccgcg aaattcgaga gcaagccggc ccaggcggca ccgatggcaa
     1021 tgccagcgcc agcgacaagc gccgcgaagg tcgtcgtctg cattccgaag taaccgagga
     1081 tgccgatcac caacaggatg ttcaaagtca cggtgacaat cgaaccgacg tagcgcaaca
     1141 ccgtcgggtc gaccttctgc cgctccagcg ccgcacgcac catgccgacc gcaatgccga
     1201 tcagccagcg gccaaccacc cagaaaacaa tcgccgccag caccttgaga ccgacatcgg
     1261 tcgccgccgc caacagccag tcctgataac gggataaact ttgcgcatcc atgatcaagc
     1321 tcctgaacga taaggggaaa atcccgcttt gattttagcg gggctaatcc atgaaagctt
     1381 gctgactaca taacttcacc attcaacgga caggcttaca taggcaacac tcaccaaact
     1441 tgttcgcaga cgttgacgac aagcggcgac ccccatataa tgcgcgcttc ccgtggggcg
     1501 ggtcggtagc tcagtcggta gagcagcgga cttttaatcc gttggtcgcg agttcgagtc
     1561 tcgcccgacc caccaggaat aattctgggt cgttagctca gctggtagag cagcggactc
     1621 ttaatccgta ggtcgagtgt tcgaatcact cacgacccac cagaattcaa cgacttaggc
     1681 cgcctccggg cggcctaagt cgtttctgac ggctgatttg ctgcatttct ggcgcccccc
     1741 ccaagtaagc cagggcaaaa aaaacgggcc cgttcaaggg gcccggtgtt ttttagcagt
     1801 gcttgcctct tacttgcgcc cgaatttctc ggccagttcc agcagcttct tctgcttcat
     1861 ggcttcggcc ttggcggcgg cttcgctggc cttgcgctgt tcggcctgct tcttgaagcg
     1921 ttccttcagt tgctcggttg catgctggcg attctcgtcg gtgacttcgc cggcaggcgt
     1981 accgtcgaga ttgaggcgtt gcgccgcctt ttccatttcc ttgaggtagc gcgtgctcat
     2041 cgtgtggctg cgcatcgcca gccgtaccga cttgcggctc aggtccggca gcgcagcgaa
     2101 caactgcttg tcgatgccga ttgccagcgg actgtagttg cggaatacgg cgaagcgctc
     2161 ctgcagttcc ttgagcaagg cacgaacgtc ggtggttttt tcgggggtgg cggaatcggc
     2221 ggtggtcatg atcgacagcg ggcaggacaa ggggcgaagc gtagcatgaa agcgccgccc
     2281 cgtgccccgg aatggcgttt acacgccatt cctgcgaaac cagtcgagca tccggcgcca
     2341 gccgtcctcg gcttcttcct tgcggtagct cggccggtaa tcggcgtgga aggcatgcgg
     2401 cgcgttgtcg tagagctgga gctgcgaagc ctttgccgcc gggctgccgg tcttcagcgc
     2461 cgcttccatt gcatcgacgg tatcgaccgg gataccggta tcgacgccgc cgtagaggcc
     2521 gagcaccggc gccttcagtt cgccgaccag gtcgatcgga tggcgcggat tgtaggcatt
     2581 gaccgggctg accaggcggc cgtaccaggc gacgccggcc ctgaccccgg gattgtgagc
     2641 gctgtacagc caggtgatgc ggccgcccca gcagaaaccg gtgatcgcca gccgaccggt
     2701 gtcggcgccc tggccggcgg cccagacgac cgccgcgtcg aggtccttca tcacctcggc
     2761 gtctggtgtc ttgctgctga tcttgtcgat gatctcggca acgctctcaa tctggcgggg
     2821 atcgccctgg cgggcgaaca gttcaggggc gacgacgaaa tagccgagtt tggccaggcg
     2881 gcgcgcggta tcgcggatgt actcatgaac gccgaagatc tcggagatca gcaacaccac
     2941 cggcgccttg ccaacgccgg ccggcgtggc gcggtaagcg accatctcac catcgtgtac
     3001 cggcaccttg acctcaccag cggtgaggcc ggcggcatcg gtgtggattg cggtctgcgc
     3061 cgccaccggc tgggtggcca gcgcaaatcc ggcccccaga ctggtaacca gaaaatcacg
     3121 ccggctgaaa ccttgcgccg gaaccaggct gtcgaattct gcggtgtcgt tcatgcctct
     3181 ctccttggct aatcacatca ggacgatgtc gtactgttcg ggtgaataac tgggctctga
     3241 ctgcagggaa accgccttgc cgatgaagtc ggagagcatc gccagcgatt gcgactcctc
     3301 gtcgagaaac aggtcaacga cggccggacc agccaggatg cggtactcgc gggcgttgaa
     3361 ctggcgcgct tcgcgcagca gttcgcggag gatctcgtag gcgacagtgc gtgccgtctt
     3421 cacctcgccg cggccgccgc aggtcgggca cggctcgcac agcacatgcg ccagcgattc
     3481 gcgggtgcgc ttgcgggtca tctcgaccag gccgagatgg gtgaagccat tcaccgtcag
     3541 ccgcgtgtgg tcgcgcgaca gcgccttgtt gaactcttcc agcaccgact tcttgtgctc
     3601 ctcgttttcc atgtcgatga aatcgacaat gatgatgccg ccaaggttcc gcaagcgcag
     3661 ctggcgggcg atggtgaccg cggcttcgag gttggtcttg aagatggtgt cgtcgaagtt
     3721 gcgcacgccg acgaagccgc cggtattgac gtcgatcgtc gtcatcgcct cggtctggtc
     3781 gatgatcagg taaccgccgg acttcaggtc gacgcggcgg gccagcgcct tctggatttc
     3841 atcctcgacg ccgtgcaggt cgaacaacgg ccgctgaccg atgtaatgct cgagcaacga
     3901 ttgcaccgcc ggcgtgtatt cgccagcgaa cagcgagagt ttctggaagt tctcccgcga
     3961 gtcgatgacg atgcgcgtcg tctccgggtt gacaaaatcg cgcagcaccc gctggccaag
     4021 cgacagttcc tggtagagca gggtgggcgg agcgctgcta cgcgaacgct ggcggatgtc
     4081 ggcgcagatt ttcttcaggt aggcgatgtc ggtggcgaat tcttcgtcgc tggcgttctc
     4141 ggccatcgtc cggacgatga agccgccggt ttcgtcgtcc ggcaccagcc gggtcagacg
     4201 ttcgcgcagc agttcgcgct cggtttccga ctcgatccgc tgcgagatgc cgatgtgctt
     4261 ctcctgcggc aggtaaacca gcatgcggcc ggcgatcgaa agctgcgtcg tcagccgcgc
     4321 ccccttggtg ccgatcgggt ccttgaccac ctggacgacg atgctctggc cttcggccag
     4381 gattttctcg atcggccgct cggtcgccga ctcgcgcggc tgccagatgt cggcgacgtg
     4441 caggaaggcg gtgcgctcga ggccgacatc gacgaaggcc gactgcatgc cgggcaggat
     4501 acggacgatg cgtcccagat agacattgcc gaccagaccg cggctggcgg tgcgttcgat
     4561 gtggagttcc tggacgaccc cttgttgcat cacggcaaca cgggtctcct ggggcgtgaa
     4621 gttgatcagg atttcttcgg tcatgggagt aaaattcgcg gtttttcaag attttactat
     4681 gcgcaaggcg ccagaactcc tcgccccggc cggttcgccc gaaatgatgc gcaccgcctt
     4741 cgccttcggc gccgacgcga tctacgccgg ccaaccccgc tacagcctgc gcgtacgcaa
     4801 caacgccttc ggcgacctgg cgatcctcgg tgacgccatc gccgaagccc atgccgccgg
     4861 caagcagttc ttcgtcgtca gcaacatcta cccgcacaac gccaagctga agacctacct
     4921 cgacgacatg gcgccagtca tcgcgctcca gccggacgcg ctgatcatgt ccgaccccgg
     4981 tctgatcgac atggtgcgca cgcgatggcc ggaacaggta atccatctgt cggtgcagtc
     5041 gaacacggtg aattgggcgg cggtcaggtt ctggcagaag atggacatca gccgcgtcat
     5101 cctgtcgcgc gaactgtcgc tcgacgaggt cgccgaaatc cgccagcaat gcccggacat
     5161 cgagcttgag gttttcgtgc atggcgcgct gtgcatcgcc tactccgggc gctgtctgct
     5221 ttccggttat ttcaaccacc gcgaccccaa tcagggcacc tgcaccaact cctgtcgctg
     5281 ggactacaag gtatcgacct cggatggcca gccggtcaat ttttaccaga acccggagca
     5341 ggaaatcctg cttgaggaac gccagcgccc cggtgaactg atgccgatcg aggaagacga
     5401 gcacggcacc tacatcatga attcgaagga cctgcgcgcc atcgagcatg tgcatcggct
     5461 gaccgagatc ggcgtcgatt cgctgaagat tgagggccgc accaagagcc cgtactacgt
     5521 cgcccgtacc tgccaggcct accgccaggc gatcgacgat gccgtcgccg gccgcccctt
     5581 cgacccgacc ctgctcggcg aactggaaaa cctggccaac cgcggctaca ccgacggctt
     5641 ctaccagcgt caccacgatg ccgactatca gaactacctg cgcggccatt ccgaatcgga
     5701 caagagccag tacgttggcg acgccgtcgc cttcgacgcg gcgcgcggct tgatgcgggt
     5761 tgccgtcaag aaccgtttcg cactcggcga caccgtcgaa tgcgtgcagc cgcggggcaa
     5821 cctgcggtca cgcatcggcc gcctggaaaa cgcggccggc gaacccgttc ccgtcgcccc
     5881 gggcagcggc cacacggtgt ggatcgactt gcccgccgag acagccggcg ccttcgtcag
     5941 ccgcttgttc tgaaacgggt tttgtgcacc gcagcagttg gcgtaatatt accgtcatga
     6001 aaccgacaac ttcgcgcatg cctctgcttg atgcactcaa ggccggcgcc tcgcagcttg
     6061 tccttttgca ccatttcgcg ctctacggcc cgctggccgt ggctttcggc gtgaccttcc
     6121 cggatctcgg cgcctggctg gtcgattacg gacggatggc ggtccaggtc ttcctc
//
LOCUS       DAOSDX010000037         6157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4593_length_6157_cov_2.947394, whole genome shotgun sequence.
ACCESSION   DAOSDX010000037 DAOSDX010000000
VERSION     DAOSDX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4593_length_6157_cov_2.947394"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PLN02_01430"
     CDS             <1..1256
                     /locus_tag="PLN02_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI51010.1"
                     /translation="GLIEARPKSGYFVRARLGQKLPEPAMTHPAGGATELQVSDFIFQ
                     ILDSVRDPGIVPLGSSFPAAALFPFDKLGRFLAGAARRLDPQSTLSDLPPGNEELRRQ
                     IALRYLAQGASVSPQEIVVTAGAMEGLNLCLQAVTRPGDLIAVESPTFYAGLQASERL
                     GLRVIEIPSHPREGVSLAALEAALRQHPVKACLFMLNFANPTGSRLSDPRKQELLQLL
                     RRHQVPLIEDDVYGELYFDRQAPLCSKAADSDGLVMHVSSFSKCLAPGYRLGWVAAGR
                     YAEAIRRQKLATSLATSVPVQIAIADYLRQGGFDKHLRQLRRRLAEQAATLVAAVERH
                     FPEGTRLTRPQGGYFLWLELPPQVDALRLHREALARGISVAPGPIFSAKREFAHHLRL
                     NFGHPAGDAQLAAVATLGRLVAAAL"
     gene            complement(1466..2158)
                     /locus_tag="PLN02_01435"
     CDS             complement(1466..2158)
                     /locus_tag="PLN02_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOI51011.1"
                     /translation="MTQELEVIIVHLVGAVLAGGVIGLERSFHARPAGFRTHTLVCLA
                     SSLLMLVTVYQWKWLPGVPMETVRTDPTRMAQGIMTGIGFLGAGVIFKEGLSVRGLTT
                     AASIWITAAIGILIGVGFYGPALIATVLTLGVLSAFRWIEAKLPSHAYAHHYIRFSRD
                     DVMPESEVRAFLLEHGFTIANMSYRITEDGASFEYKMVIRTNNAANTAKLVEALRDIK
                     RIRAFRVSPTGD"
     gene            complement(2516..2839)
                     /locus_tag="PLN02_01440"
     CDS             complement(2516..2839)
                     /locus_tag="PLN02_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51012.1"
                     /translation="MNTDELILAIIAEMERVWGGVCNFHGDNGDYQWLQENYGISEED
                     DVRWQLFLEYDADDLSEEDREDEELMAFLEDADAVEQFSQSMLARYRSSSAVSLKVTG
                     TKKTA"
     gene            complement(2943..3455)
                     /locus_tag="PLN02_01445"
     CDS             complement(2943..3455)
                     /locus_tag="PLN02_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51013.1"
                     /translation="MTPTAPAPRLLAPTLAWLALLALTFVSLLLGRWLHGSPALPWLV
                     AAIVWVKGWLICHCFLEAPLAHPFIRRVLAGFVLAVPVALVQVALFALFGATGTASTK
                     PASTRYRHREQKVSGSTRPQAVTGHPVRIAVIRARRYATGLYAKSVAPTRNSGAQPRI
                     TPRLHPKEES"
     gene            complement(3462..3908)
                     /locus_tag="PLN02_01450"
                     /pseudo
     CDS             complement(3462..3908)
                     /locus_tag="PLN02_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
     gene            4092..5474
                     /gene="ccoG"
                     /locus_tag="PLN02_01455"
     CDS             4092..5474
                     /gene="ccoG"
                     /locus_tag="PLN02_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOI51014.1"
                     /translation="MNKPVSKVREAKLELYRKHGKIHARSVEGRFNRWRWGMVWFTQA
                     IFYGLCWLPWESEAGRRQAVLFDIAHEKLYLFDLVLWPQDALLLAFVLILAATALFLT
                     TALAGRLFCGFACPQTVYTMLFTWIEARVEGDHLARLKLDRSPPGPRKLALRLTKHLL
                     WAALAAWTAITFVGYFTPIRELLPSIAALSTGPWEAFWLIFYAAFTYVQAGLAREAVC
                     QHMCPYSRFQGVMFDPATRNVAYDRQRGEPRGSRGAGHGDCIDCGICVEVCPTGIDIR
                     DGLQYQCINCGLCIDACDRVMDRIGAPRGLIRFASENELSGRAGAGWRQRPRLAVYAG
                     VLVAVAGLAAWTLAERSLLRVDVLRDRGALARETADGRIENAYTLKLVNIADRPRRFT
                     VAVAGPLPLEIVGASEFAVGAGSLVPVAVTVAAPAGEQRAGIVPIRFAVSAVDDPALR
                     VDEASSFALP"
     gene            5471..5686
                     /locus_tag="PLN02_01460"
     CDS             5471..5686
                     /locus_tag="PLN02_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51015.1"
                     /translation="MTRHEDLPPLWRRADGRPLACVEKIRVLDENYRELAQVLRDALE
                     DGILLGCAESALRAALHELVERVRPGF"
     gene            complement(5692..>6157)
                     /locus_tag="PLN02_01465"
     CDS             complement(5692..>6157)
                     /locus_tag="PLN02_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51016.1"
                     /translation="APLPGLLFRHREADGEHFVYVVDAASGRLAGYTVFNRLIEIDRR
                     SDRHVRSPHSKYAPAWQGRGLASAVYAWALARGICLVSGARQSPAAFALWRSLGRRQP
                     LRYVDIRDKRIHDLGPTVDAAQHDQLTTRMFLAGNGWSALRLRRAGVLHDAR"
BASE COUNT         1067 a         2020 c         2018 g         1052 t
ORIGIN      
        1 gcggcttgat cgaggcgcgg ccgaaatccg gctatttcgt gcgcgcccgc ctcggccaga
       61 agctgcccga accggcgatg acgcatccgg ccggcggcgc caccgagcta caggtcagcg
      121 atttcatctt ccagattctc gacagcgtcc gcgatccggg catcgtgccg ctcggctcga
      181 gctttccggc ggcggcgctg ttccccttcg acaagctcgg gcgtttcctc gccggggccg
      241 cccgccggct cgatccgcag tcgacgctga gcgacctgcc gccgggcaac gaggaattgc
      301 gccggcagat cgcgctgcgc tacctggcgc agggcgcctc ggtttcaccg caggagatcg
      361 tcgtcaccgc cggcgcgatg gaaggcctca acctctgcct gcaggcggtg acccggccgg
      421 gcgacctgat cgccgtcgag tcgccgacct tctacgccgg cctgcaggcg agcgagcgcc
      481 tcggcctgcg cgtcatcgag attcccagcc atccgcgcga gggcgtcagc ctggcggcgc
      541 tcgaagcggc gctgcgccag catccggtga aggcctgcct gttcatgctc aacttcgcca
      601 acccgaccgg cagccgcctg tccgatccgc gcaagcagga actgctgcag ttgctgcgcc
      661 gccaccaggt gccgctgatc gaggacgatg tctacggcga actctatttc gaccgtcagg
      721 cgccgctgtg cagcaaggcc gcggacagcg acggcctggt catgcacgtt tcctcgttct
      781 ccaagtgcct ggcccccggc taccgcctcg gctgggtcgc cgccggccgc tacgccgagg
      841 cgatccgccg gcagaagctg gcgaccagcc tggcgacctc ggtcccggtg cagatcgcga
      901 tcgccgatta cctgcgtcag ggcggcttcg acaagcacct gcgccagttg cggcggcgcc
      961 tggccgagca ggcggcgacg ctggtcgccg ccgtcgagcg acatttcccg gaaggcaccc
     1021 ggctgacccg gccgcagggc ggctattttc tgtggctcga actgccgccg caggttgatg
     1081 cgctgcgcct gcaccgcgag gcgctggcgc gcggcatcag cgtcgcgccg gggccgatct
     1141 tctcggccaa gcgcgagttc gcccaccatc tgcggctgaa tttcggccat ccggccggcg
     1201 acgcgcaact ggcggcagtg gcgaccctgg gccgcctggt cgcggctgcg ctgtagccgc
     1261 agaatcgcac tgatcagacc tgagtcgcat caacttgacc ggctcgaatc gacccaaacc
     1321 ggaaatccga ctccccaact gcatatgtcc gctaccgaag gaagtctgga ccttcaaggg
     1381 tcttccttgg tggcccgtgc ttctgccaca gttaatggtc agaaggtcgg ttaaacctgg
     1441 ggaaattcag attgccgctc gggaatcaat ccccagttgg ggagacacgg aatgcacgga
     1501 tacgtttgat gtcccgcaac gcttcgacca gcttggccgt gttggcagca ttgttagtcc
     1561 tgatgaccat cttgtactcg aacgatgcgc catcctccgt gattcggtag ctcatgttcg
     1621 caatcgtgaa cccatgctcc agtaagaagg ctcggacctc actctccggc atcacatcat
     1681 ctcggctgaa gcggatatag tgatgggcgt aggcatgaga aggcaacttc gcttcaatcc
     1741 accgaaaggc cgacaggaca cctaaggtca gcacggtggc gataagggcc gggccataga
     1801 aacccacccc aatcaagatg ccgatagctg ccgtaatcca gatggaggct gccgtggtca
     1861 gaccccgaac actcaagccc tccttgaaaa tcactccagc acccaggaag ccgatgccgg
     1921 tcatgatgcc ctgggccatg cgggttgggt ctgtccttac agtttccatt ggaacgccag
     1981 ggagccactt ccactgatag accgtaacca gcatgagcag acttgaagca aggcagacca
     2041 gggtatgggt acgaaaacca gcaggtcgtg catggaagct ccgctccagg ccaataaccc
     2101 ctccggctaa taccgcaccg acaaggtgaa caatgatgac ttccaactct tgtgtcattg
     2161 tcagtctcca aattcttcag gctatcgtaa caccgacggc cccggctggc cgctctaggg
     2221 ttattcgtcg ggtgcagaac gaccgttgag ccgcctatag cagacgggct gactgtggtt
     2281 ttagttatga gtggatgtcg gcaagtgggc cgaaagcgga catcatagcg ccatgcctaa
     2341 tggatggcga caggccgcaa ctggccgatt gctgcccggc cggcatcttt caactctgca
     2401 agccgcgcga ccaccaggat gcccccattt ggcgaattcc ccgcgaggat aaccgggcag
     2461 tgctgtgctc tctgggaagg gagttgggtt gagactgctc ggcctggtcc tgatctcagg
     2521 cggtcttctt tgtgccagtg accttcagtg agaccgcaga agagctgcga tacctcgcaa
     2581 gcatgctttg cgagaactgt tcaacagcat cggcgtcctc caagaacgcc attagctctt
     2641 catcttctcg gtcttcttcg gagaggtcat cggcatcgta ttcgagaaac aactgccagc
     2701 gaacatcgtc ttcttcagaa atgccatagt tttcttgcag ccactggtaa tcaccgttgt
     2761 cgccatgaaa attgcagaca ccgccccaca cccgctccat ctcagcaata attgccaaaa
     2821 tcaattcatc tgtattcact ggagttttcc tttagaaaat caattgccgc cgacttgctt
     2881 tgcagcagcg acgtgcatgc cttcgatcca gacgtttgag ttgtcgacgt agagaagatt
     2941 ggtcacgatt cctccttagg gtgtaagcgc ggtgtgatac gaggctgggc gcctgaattg
     3001 cgggtcggtg cgaccgattt ggcatacagg ccagtagcat accgtctcgc ccggatgacc
     3061 gctatccgga ctgggtgacc agtgacagct tgtggccggg tgctgccaga gaccttctgc
     3121 tcacgatgac gatatcgcgt gcttgccggc ttcgtgctgg cggtgccggt ggcgccgaac
     3181 agggcgaaca gggcaacctg caccagcgcc accggcaccg ccagcacgaa gccggcaagc
     3241 acgcggcgga tgaacggatg cgccagcggc gcttcgagga agcagtggca gatcagccag
     3301 cccttgaccc agacgatcgc ggcgaccagc cagggcagcg ccggcgagcc gtgcagccag
     3361 cggccgagca gcaggctgac gaaggtcagc gcgagcagcg ccagccaggc cagggtcggc
     3421 gccagcaggc gtggggcggg ggcggtcggg gtcatggttc aggcgagcac ccagaacatg
     3481 ccgaggatgc cccagaccgc atgcaccagg tggttgaagg tcaggtagta gtagacggtg
     3541 aagaagatgc cggattcgcc gttgatgccg tgggcgaggt tccagcggat ctcgaggaac
     3601 ttggccagca ggctgccgct cagccagtac agcgactgcc gccggcgacc ggcgcggatg
     3661 gcctgcaccg cgcaggcgat caggaagctg ctgctgagca tgagcagtgt gattgccgtg
     3721 ccggcggtgc gcgacaggcg ctcgccgccc tcggcgaaag cctcggggaa atgcgcgcgg
     3781 gcgacgaagt agaccgtgaa cagcacgaga aactcgacga actcgcagca gatgccgacc
     3841 cagattccct tgttaccggg gatgcgtccg cccggcgcgg cgtgtgtggc gagggcggcg
     3901 gttgccatga tcttgcgacc gccatcgtgc catcgccggc ggcggcgcac agggacagat
     3961 ggcgcgaaag gcgatcgcta cagggagctg gtagtcaaat ctgtgcctat aacaaatcgc
     4021 agtttctgaa actgttcctg tctggcggcc ggtggtctac tggcgagcgt caaacatcaa
     4081 ggagcgggag catgaacaag ccggtcagca aggtcaggga agccaagctg gagttgtatc
     4141 gcaagcatgg gaagatccac gcccgctcgg tcgagggccg tttcaaccgc tggcgctggg
     4201 gcatggtctg gtttacccag gcgatcttct acggtctctg ctggctgccc tgggagagcg
     4261 aggccggacg ccggcaggcg gtgctcttcg acatcgcgca cgaaaagctc tacctgttcg
     4321 atctcgtgct ctggccgcag gatgcgctgc tgctcgcctt cgtgctgatc ctggcggcca
     4381 ccgcgctgtt cctgacgacg gcgctggccg gccggctgtt ctgcggcttc gcctgtccgc
     4441 agacggtgta cacgatgctg ttcacctgga tcgaggcgcg cgtcgaaggc gatcacctgg
     4501 cccgcctgaa gctcgaccgc agcccgcccg ggccgcgcaa gctggcgctg cgcctcacga
     4561 agcacctgct gtgggcggcg ctggcggcgt ggacggcgat caccttcgtc ggctacttca
     4621 cgccgatccg cgagttgctg cccagcatcg ccgcgctgtc caccggcccc tgggaagcct
     4681 tctggctgat cttctacgcc gccttcacct acgtccaggc cgggctggcg cgcgaggcgg
     4741 tgtgccagca catgtgcccg tattcgcgct tccagggcgt gatgttcgac ccggccacgc
     4801 gcaacgtcgc ctacgaccgc cagcgcggcg aaccgcgcgg cagccgcggc gccggccacg
     4861 gcgactgcat cgattgcggc atctgtgtcg aggtctgccc gaccggcatc gacatccgcg
     4921 atggcctgca gtaccagtgc atcaactgcg gcctctgcat cgacgcctgc gaccgggtga
     4981 tggaccggat cggcgcgccg cgcgggctga tccgcttcgc ttcggagaac gagctgagcg
     5041 gccgtgccgg cgccggctgg cggcagcggc cgcgcctggc ggtgtacgcc ggcgttctcg
     5101 tcgccgtcgc cggcctcgcc gcgtggacac tggccgagcg ttcgctgctg cgcgtcgacg
     5161 tcctgcgcga ccgcggcgcg ctcgcccgcg aaaccgccga cggccggatc gagaacgcct
     5221 acacgctgaa gctcgtcaac atcgccgacc ggccgcgccg cttcactgtt gcggtcgccg
     5281 gcccgctacc gctggaaatc gtcggcgcca gcgagttcgc ggtcggcgcc ggcagcctgg
     5341 tcccggtcgc ggtcaccgtc gccgcgccgg ccggcgagca gcgcgccggc atcgtgccga
     5401 tccgcttcgc ggtcagcgcc gtcgacgacc cggcgctgcg cgtcgacgaa gcgagcagct
     5461 tcgcgctgcc atgacccgtc acgaggattt gccgccgctc tggcggcgcg ccgacggccg
     5521 gccgctggcc tgcgtcgaga agatccgcgt cctcgacgaa aactaccgcg aactggcgca
     5581 ggtgctgcgc gacgcgctcg aggacggcat cctgctcggc tgcgccgaaa gcgcgctgcg
     5641 ggcggcgctg cacgaactgg tcgagcgcgt ccgcccgggt ttctgagacg ctcatcgggc
     5701 gtcgtgcagg acgccggcac ggcgcaggcg gagtgccgac cagccgttgc cggcgaggaa
     5761 catgcgcgtc gtcaactggt cgtgctgcgc ggcgtccacc gtcggtccga ggtcatggat
     5821 gcgcttgtcg cggatatcca cgtagcgcag cggctggcgg cggccgagcg agcgccacag
     5881 ggcgaaggcg gccggcgact ggcgggcgcc gctgaccagg cagatgccgc gcgccaacgc
     5941 ccaggcatag acggcgctgg ccaggccgcg cccctgccag gccggtgcgt acttcgaatg
     6001 cggcgagcgg acgtgacggt cgctgcggcg gtcgatctcg atcagccggt tgaataccgt
     6061 atagccggcg agccggccgc tggcggcatc gacgacatag acgaagtgct cgccgtcggc
     6121 ttcgcggtgg cgaaagagca ggccgggcag cggcgcc
//
LOCUS       DAOSDX010000038         6146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4603_length_6146_cov_3.516171, whole genome shotgun sequence.
ACCESSION   DAOSDX010000038 DAOSDX010000000
VERSION     DAOSDX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4603_length_6146_cov_3.516171"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /gene="ampD"
                     /locus_tag="PLN02_01470"
     CDS             <1..396
                     /gene="ampD"
                     /locus_tag="PLN02_01470"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HOI51017.1"
                     /translation="DPAAHPYFAEIADLQVSAHFYVRRDGRVVQFVGCDRRAWHAGRS
                     TWCGRDNCNDYSVGIELEGCDEQAFTDAQYAALWRLIDALRQRYPIAAIAGHSDIAPG
                     RKSDPGPYFDWAALAARYPDLGLPPAVTA"
     gene            complement(543..1379)
                     /locus_tag="PLN02_01475"
     CDS             complement(543..1379)
                     /locus_tag="PLN02_01475"
                     /inference="COORDINATES: protein motif:HMM:NF007341.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOI51018.1"
                     /translation="MRPNSGASYRAVQGLSRGLSILRAISQSANGWANIAELSVRTGL
                     HRTTVRRMLETLQAEGYVRRSASDDSYRLNLKVRQLSDGFTDDEWISEIANPILGELL
                     QKVVWPSDLSTLDGDCMLVRETTHRFSPLSFHRAMIRQRMPLLFTSAGRAYLAYCSDE
                     ERRQILQLLVAGNDEQAGFARNRILVEQMLEKVRRQGYATNEGEWGQQSMIAAIAVPV
                     RHKAQVLACINVVFLKKAVPIAEATERYLPALKAAVDKIELLLSDKDATSATEESNAM
                     AD"
     gene            1669..2607
                     /locus_tag="PLN02_01480"
     CDS             1669..2607
                     /locus_tag="PLN02_01480"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HOI51019.1"
                     /translation="MTIHLKCLSHTPLRGLNDPGADVEVEVDAVLAKAKAEVEAFDPE
                     LIIIFAPDHYNGLFYDMMPPFVIATAAESVGDYKTLLGALSIPKDLALDLARHILDSD
                     VDIAISHRLQVDHGCTQTLEELTGGLTRYPVIPIIINSVAPPFGPYRRVRKLGDAVGK
                     FVAKLNKRVLILGTGGLSHEPPVPLLAGANDDIANFLIAGRNPTPEFRAARQARTLAT
                     GMIFGTPECDLTPLNPEWDEDFMDLLVRGQLDRCDAFDIDEISRMAGRSTHEIRTWVA
                     AFAALAAIGPYNAHRDYYRPINEWIAGYGVLSASPK"
     gene            2660..3868
                     /locus_tag="PLN02_01485"
     CDS             2660..3868
                     /locus_tag="PLN02_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOI51020.1"
                     /translation="MTTHTIPSEAEIVQRAHDMIPMLRARAEECERNRMVPVETIQAF
                     KEAGFFRILQPKRWGGYEMSPNVLNKVLIELARGCPSSAWNVMVLGVHPFEVGLLPVK
                     VGDELWGEDNTQLVSSSYAPFGTVAKVEGGYKLNGEWLTSSGCDHAAGGAFVGGRVEE
                     NGELVFRSFWVQRSDYEIVDDWFVVGLAGTGSKKLVIKDVFVPEYRCHVIGAYDEQSH
                     GGVEKLYKMPFFYVFYAAVSSVIIGMARGMADLYIEHMVPRQNINQAVGAAVNDPFIK
                     GRLGEAYAKIAGATARVLMNTDEAWSYASNGQLVPLDVRVRHFATNQFTGGDCFEAAH
                     MIFKKTSTRGVWMNSPMQRQMRDILVGANHITQNQDNIGDLLGGQLLGNPLPQPNPFG
                     AKPAQAAQYQ"
     gene            3878..4429
                     /locus_tag="PLN02_01490"
     CDS             3878..4429
                     /locus_tag="PLN02_01490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOI51021.1"
                     /translation="MSQDINQLLDWKPNTRLAAMPTVSAEDHRYGMRHFAVGVTVITA
                     RDGDARAGLTATAVCSVTADPPRLVVFVNKNVMASEVILNSGALCVNVLAGEQEEVAK
                     VFAGMIKEVHGDARFQYGAWRELVTGAPSLDGALANFDCRVIKVFDESTHHAFLCEVL
                     ATCERNDGEALIYLNGAFRRIPQ"
     gene            4471..5604
                     /locus_tag="PLN02_01495"
     CDS             4471..5604
                     /locus_tag="PLN02_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI51022.1"
                     /translation="MKTNKLIAAVVAAIACVPAVADVNVGVIASLTGPAAALGAETKK
                     AVALFPNTLGGEKVNFILLDDGTDPTNATKNVRKLISEDKVDVILGPNLIVTANAMAD
                     VANADKTPMVSVAPLEVAGDKRGYVFRSEPSAELMVNKVVADMLEGGAKTVGFIGFSD
                     SWGELLHKALIKAAEGKLKIVASERYGRADPSVQAQVLKVMSARPDVVFVGASGTPAA
                     MPQITLRERGFKGKIYQSHGVTNKEFLRVGGKHVEGALIPVGPVLVAEQLPDNYPTKK
                     NAVAFVKDLEARNGPDSRSTFAGATWDAWLLAQNGLQAALKGKAKPGTPEFRAAVRDG
                     IEKSNKLVGTNGVYTMGANDHAGYDASAIVLIKVENNHWKLVK"
     gene            5604..>6146
                     /locus_tag="PLN02_01500"
     CDS             5604..>6146
                     /locus_tag="PLN02_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HOI51023.1"
                     /translation="MGEPLTPQAAAALLKAAHAGGRAIAPLRERLARVDQEGAYAVQE
                     ANTLAWLGEGRRLVGRKIGLTSLAVQAQLGVDQPDFGMLFADMAVGDGEIVATGRLIQ
                     PKVEAEVALVIGRDLTHERHTYADLISATEYALPAIEIVDSRIENWNIKFVDTVADNA
                     SSGLFVLGGRPVRLRDLDLTA"
BASE COUNT         1146 a         1963 c         1876 g         1161 t
ORIGIN      
        1 gatcccgcgg cccaccccta ttttgcagaa atcgccgacc tgcaggtttc ggcgcatttc
       61 tacgtccgcc gcgacgggcg cgtcgtccag ttcgtcggct gcgaccggcg cgcctggcat
      121 gccggccgct caacctggtg cggacgcgac aactgcaacg actactcggt cggtatcgaa
      181 ctcgagggct gcgacgagca agccttcacc gacgcccagt atgcggcgct atggcgcctg
      241 atcgacgcct tgcgccagcg ctatccgatc gccgccattg ctggtcacag cgacatcgcg
      301 cccgggcgca agagcgatcc cgggccgtat ttcgactggg cggcgctcgc cgcccgctac
      361 cccgacctcg gcttaccgcc cgcagtgacg gcgtaggcgc gcgaggtgcg atcagggccg
      421 ccgggcgagc ctggcagcgg acctgatgcc ttgtgcttcg gcacggatgg aacctctatt
      481 cgcttgaaac ccgtcctcgt ttcccacttt tgtcgctgcg acccatcaag tccgagcggc
      541 ggctagtcgg ccatggcgtt tgattcctcg gtggccgagg tggcgtcctt gtcgctcagg
      601 agcaactcga tcttatccac cgcggctttc agcgctggca gatatcgttc tgtggcttcc
      661 gcgatgggta cggctttctt caggaagacg acgttgatgc atgccaatac ctgagccttg
      721 tggcgaaccg ggacggcgat ggcggcgatc atcgattgct gcccccattc tccctcgttc
      781 gtcgcgtagc cttggcgtcg cactttttca agcatctgtt cgaccagtat ccggttgcgc
      841 gcaaagccgg cctgctcgtc gttgcctgct accagtagct gaagaatctg ccgcctttcc
      901 tcgtcgctgc aataggccag ataggctcgc ccggcagaag tgaacaggag gggcatgcgt
      961 tggcggatca tcgcccgatg gaaagagagg ggactgaagc ggtgcgtggt ttcccgcacc
     1021 agcatgcagt cgccgtcgag cgtgctgagg tcggaaggcc agacaacctt ctgcagcaat
     1081 tcgccaagga tcggattggc gatctcggaa atccattcgt catcggtgaa gccgtcgctc
     1141 aactggcgaa ctttcaggtt caggcggtag ctgtcgtcgg aggcgctgcg ccggacatag
     1201 ccttcggcct gcagggtttc cagcatgcgg cgcacggtgg ttcggtgtag tcccgtgcga
     1261 acgctcagtt cggcgatgtt cgcccagccg ttggcggact ggctgattgc ccgcaggatc
     1321 gacagcccgc ggctcaagcc ttgtacggcg cgatagctgg ctccactgtt cggtcgcatc
     1381 gcattccctt tgttcgataa aaaccatatt taaaacaatg ttgtgcatat tgtgaacatt
     1441 tgggaaataa atttttgttc tcccttgtct ctccctagaa tcgccccatc aacaaaaaca
     1501 tatggagaca aacatgacta tcgagtcgac gggcgacggt taaacctcgg cccgcttgtt
     1561 ggaagcggcg gcttcccttt caagaatcca agaatcgtct ttcgctgcgg aagatttcgc
     1621 tcttccggcg ggagcggctt gcccgaattt accgatcaag gataacgcat gaccattcac
     1681 cttaaatgtc tgtcgcatac accgttgcgc ggactgaacg acccgggcgc cgatgtcgaa
     1741 gtcgaagtcg atgccgtgct ggccaaggcc aaggccgagg tcgaggcttt cgacccggaa
     1801 ctgatcatca tcttcgcgcc ggaccattac aacggcctgt tctacgacat gatgccgccg
     1861 ttcgtcatcg ccacggcggc cgagtcggtc ggcgactaca agaccctgct gggggcgttg
     1921 tcgatcccca aggatctggc gctcgacctt gcccgccaca ttctcgacag cgacgtcgat
     1981 atcgccatct cgcaccgcct gcaggtcgat cacggctgca cgcaaacgct ggaggaactg
     2041 acgggggggc tgacgcgcta cccggtcatc cccatcatca tcaactcggt ggcgccgccg
     2101 ttcgggcctt atcgccgggt gcgcaagctc ggcgacgcgg tcggcaagtt cgtcgccaag
     2161 ctgaacaaac gcgtgctgat cctcggcacc ggcgggcttt cgcacgaacc tccggttcct
     2221 ctgctggccg gcgccaacga cgacattgcc aatttcctga ttgccggccg caatccgacg
     2281 ccggagttcc gtgccgcccg tcaggcgagg acgctggcaa ccgggatgat cttcggcacc
     2341 ccggagtgcg acctgacgcc gcttaatccc gagtgggacg aggatttcat ggacctgctg
     2401 gtccgcggcc agctggatcg gtgcgatgcc ttcgatatcg acgagatatc gcgcatggcc
     2461 ggccgttcga cgcacgagat ccgtacctgg gtggcggctt tcgctgctct ggccgccatc
     2521 ggtccctaca acgcccatcg cgattactac cgtccaatca acgaatggat tgctggctac
     2581 ggcgtcttga gcgccagccc gaagtgaatt cattcccgaa aaatctgcat cacgccccct
     2641 gcccaaggag atgtaagaca tgactaccca taccattccg agcgaagccg aaatcgttca
     2701 acgtgcgcac gacatgattc cgatgctgcg cgcccgcgcc gaagaatgcg aaagaaaccg
     2761 catggtgccc gtcgagacca tccaggcctt caaggaggcg ggtttcttca ggattctgca
     2821 gcccaagcgc tggggcggct acgaaatgtc ccccaatgtg ctcaacaagg tgctgatcga
     2881 actggctcgt ggctgtccgt ccagtgcctg gaacgtcatg gtgctcggcg tgcatccctt
     2941 tgaagtgggc ctgctgccgg tcaaggtggg cgacgaactg tggggtgaag acaataccca
     3001 actcgtctct tcgtcctatg cgcccttcgg caccgtggcc aaagtcgagg gcggttacaa
     3061 gctgaacggc gagtggctga cctccagcgg ctgcgaccat gctgccggcg gagccttcgt
     3121 cggcggccgc gtcgaggaaa acggcgaact ggtcttccgc tccttctggg tgcaacgcag
     3181 cgattacgaa atcgtcgatg actggttcgt tgtcggtctg gccggcactg gatccaaaaa
     3241 gctggtcatc aaggacgttt tcgtcccgga ataccgctgc catgtcatcg gcgcctatga
     3301 cgagcagtcg cacggcgggg tcgagaagct gtacaagatg ccgttcttct acgtcttcta
     3361 cgccgccgta tcctcggtca tcatcggcat ggcccgcggc atggccgatt tgtacatcga
     3421 gcacatggtg ccgcgccaga acatcaacca ggccgtcggt gccgcggtca acgacccgtt
     3481 catcaagggg cgtctgggcg aggcttacgc caagatcgcc ggcgccaccg cccgcgtcct
     3541 gatgaatacc gatgaagcct ggtcctacgc ctccaatggg caactggttc cgctcgatgt
     3601 ccgcgtgcgt catttcgcga ccaatcaatt taccgggggc gattgtttcg aagccgctca
     3661 catgatcttc aagaagacct cgacccgggg cgtctggatg aacagcccga tgcagcgcca
     3721 gatgcgcgac atcctggtgg gggcgaacca catcacgcag aaccaggaca acatcggcga
     3781 cctcctcggc ggccaactgc tcggcaaccc gctgccgcaa ccgaacccgt tcggcgccaa
     3841 gccggcgcag gctgcgcagt accagtaagg agtgctcatg agccaggaca tcaaccagct
     3901 actcgactgg aaacccaata cccggctggc ggcgatgccc accgtcagtg ccgaggacca
     3961 tcgctacggc atgcgccact tcgcggtcgg agtcaccgtc atcaccgcca gggacggcga
     4021 tgcccgggcc ggcctgaccg cgacggccgt ctgttcggta accgccgatc cgccccgcct
     4081 ggtcgtcttc gtgaacaaga acgtgatggc cagcgaggtc atcctcaaca gcggcgccct
     4141 ctgcgtcaac gtgctggccg gcgagcagga ggaggtcgcc aaggttttcg ccggcatgat
     4201 caaggaggtg catggcgatg cccgtttcca gtacggcgct tggcgcgaac tggtgactgg
     4261 cgcgccatcg ctggatggcg cgctggccaa tttcgattgc cgcgtgatca aggtgttcga
     4321 cgaaagcacc caccacgcct tcctgtgcga agtgctggcg acctgcgaac gcaacgacgg
     4381 cgaagcgctg atctacttga acggtgcctt ccgccgcatt ccccagtaac cgccccgacg
     4441 ttcgacccca ttacccagag gagacttacc atgaaaacaa ataagctgat tgctgccgtc
     4501 gttgccgcca tcgcctgcgt tcccgctgtg gccgacgtta atgtcggcgt catcgcctcg
     4561 ctgactggtc cggcggccgc gttgggcgcg gaaaccaaga aggctgtcgc cttgttcccg
     4621 aatacgctcg gcggcgagaa agtcaatttc attctgctcg acgacggcac cgatccgacc
     4681 aacgccacca agaacgtccg caagctgatc tccgaagaca aggtcgacgt catccttggc
     4741 ccgaacctga tcgtcaccgc caacgcgatg gccgacgtgg ccaatgccga caagacgccg
     4801 atggtttccg tcgccccgct cgaggtcgcg ggcgacaagc gcggctacgt ctttcgcagc
     4861 gagccgtcgg ccgagctgat ggtcaacaag gtcgtcgccg atatgctgga aggcggcgcc
     4921 aagacagtcg gcttcattgg tttctcggat tcctggggcg aactgctgca caaggcgctg
     4981 atcaaggcgg ccgaagggaa gctgaagatc gtcgccagcg agcgctacgg ccgggccgat
     5041 cccagcgtcc aggcgcaggt gctgaaagtc atgtcggcca ggccggacgt cgtcttcgtc
     5101 ggcgcctcgg gtacgccggc cgccatgcca cagatcacgc tgcgcgagcg cggtttcaag
     5161 ggcaagattt accagtcgca cggcgtgacc aacaaggaat tcctgcgcgt cggcggcaag
     5221 catgtcgaag gtgcgctgat cccggtcggc ccggtgctgg ttgccgagca gctaccggac
     5281 aactacccga ccaagaagaa tgccgtggcc ttcgtcaagg atctggaagc caggaatggt
     5341 ccggattccc gctcgacctt cgcgggcgcc acctgggacg cttggctgct cgcccagaac
     5401 ggcctgcagg ctgcactgaa gggcaaggcc aagccgggca cgccggagtt ccgcgccgcg
     5461 gtgcgcgacg ggatcgaaaa gagcaacaag ctggtcggca ccaacggcgt ctacaccatg
     5521 ggcgccaatg accacgctgg ctacgacgct tccgccatcg tcctgatcaa ggtcgagaac
     5581 aaccactgga aactggtgaa gtgatgggcg agccgcttac cccccaagct gcggcggcgc
     5641 tactcaaggc cgcccatgcc ggcggccggg ccatcgctcc gctgcgcgag cgtctggccc
     5701 gggttgatca ggagggcgcc tacgccgtcc aggaagccaa taccctggcc tggctgggcg
     5761 aagggcgtcg cctggtcggg cgcaagatcg gcctgacctc gttggccgtg caggctcagc
     5821 ttggtgtcga tcagccggat ttcggcatgt tgtttgctga catggcggtt ggcgacggcg
     5881 agatcgtcgc aactggccgg ctgatccagc cgaaggtcga agcggaggtc gccctggtga
     5941 tcgggcgtga cctgacgcat gagcgccata cctatgccga cctgatcagt gccaccgagt
     6001 acgccttgcc ggccatcgag atcgtcgata gccgtatcga gaactggaac atcaagttcg
     6061 tcgacacggt ggcggacaac gcttccagcg gtctgttcgt cctcggtggt cgtccggtca
     6121 ggctgcgcga tctcgacctt accgcc
//
LOCUS       DAOSDX010000039         6096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4652_length_6096_cov_3.705347, whole genome shotgun sequence.
ACCESSION   DAOSDX010000039 DAOSDX010000000
VERSION     DAOSDX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4652_length_6096_cov_3.705347"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PLN02_01505"
     CDS             complement(<1..1033)
                     /locus_tag="PLN02_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HOI51024.1"
                     /translation="MTETFYEVLRRQGITRRSFLKFCSLTATSLGLGSAAAPRIAQAM
                     ETKPRTPVIWLHGLECTCCSESFIRSAHPLTKDVVLSMLSLDYDDTLMAAAGHQAEAI
                     IEEVKKKYKGNYIVAVEGNPPLNEDGMFCIHGGRPFVEVLKETCADAKAIISWGACAS
                     YGCVQAAKPNPTRATPVHKVISGKPIINVPGCPPIAEVMTGVVTYMLTFDRIPELDRQ
                     GRPKMFYGQRIHDKCYRRGHFDAGQFAEAWDDEGSRKGYCLYKMGCKGPTTYNACSSM
                     RWNGGVSWPVQSGHGCIGCSEEGFWDKGSFYDRVTSITPFGAESNADTIGKAAAGTVG
                     AAIAAHAAVT"
     gene            1306..5925
                     /locus_tag="PLN02_01510"
     CDS             1306..5925
                     /locus_tag="PLN02_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI51025.1"
                     /translation="MPTVADLMTLHADSVAPSATLAEAAALMVEARISSVVVVDATRV
                     VGILTERDMLHAMRRHEDQRRPVAHFMTAPVHTVSHDTEVRSAFRQATRLGIRHLVVT
                     GGEGRLLGIVSETDFRRFFGLDFYRQLNTVEGLMDRVFPRLPRQATLDEALAAMETAR
                     ATSVVVVDAGQPCGIVTERDVVRLYLQASGNPTLGEVMTQPLTSVLPETSAAEAAALM
                     LESRIRHLVVVDAEGRLLGLLTEHSLIRPLEMDLLDETISEQRGLQTRYDEALLIRNA
                     AMTGMLRGERLAGLLELIALSAEHEVPGIICSIMLADADGSHLHVVAAPGLAEDARAL
                     LANMPVAEGVGVSGAAAWRRGEAYIENIHESSAGIDYRGFARRIGITSGWAEALLGPD
                     GRLLGTFAVYREQAGRLPDGSRETIRQASRLAALMIAHQQQADELGRSQQTFRELFES
                     VGDALFVIDGDGRCLDANAAAERMTGYRREELRGHAPDFLAAQGLNDLARIAAVIRSA
                     TGGAPQELEFWGCDRDGRVLLVRLQISATQYAGKPALIVTAIDISVTRAEQLRLGIER
                     DLANALNTEADPEALLATMLDLALRYPEFDSGGFYWQNAGGDYELRVHKGLSPAFVAA
                     ARCCVADSPKAELIRNGKVLCSCHTCCQQQDHCSLIDPAHYAGEGLRCLIALPIVVNG
                     RAIACLNLAGHRTVQISVATCQAVQHLVSQYAMALERIEAREAARQSRDNLSGLFASL
                     DDLIFVIDAGGRIVHYNPVVDQALGYAPGILHGEPVSRLYPASWREQAQHNLDEMFAG
                     RLNACPAPLLAADGRLVQTEVRAVHGHWNGEAALIAVARDVGERLAAEERLRLAASVF
                     ANAHEGIMITDVSGHIVEVNPTFSELTGYSRAEAIGQNAGMLNSGHHDAAFYRNMWEA
                     IRDQGHWRGEVWNRKKSGEIFVELLTISAVRDAERTITHFVGIFSDITLLKEHQQRLE
                     RLAHFDALTQLPNRMLLADRLQLAMANAARNRGLLAVCYLDLDGFKPVNDRFGHAAGD
                     RLLVEVAQRLRNCVRGGDTVARLGGDEFVLLLSTLNDLNECDQALSRISASLTQPFAV
                     AGENVTISASIGVTLYPHDGSDADTLLRHADQAMYVAKQAGRNRHHLFDPEKDRRARR
                     RREDITRIGQGLAAGEFVLHYQPKVDMRQGRVVGAEALIRWQHPQRGLLLPQEFLPAI
                     EGNELAIDLGNWVITTALDQLSSWAAAGIDLKLSINIAGEHLQHPGFAAELGRQLAAH
                     PELDPSRLELEVLETAAIEDVGRVAQLFTECRQLGVSFALDDFGTGYSSLTYFRRLPA
                     EILKIDQSFVRDMLDDPEDLAIVEGVIGLTRAFRRQVIAEGVESIEHGLVLLLLGCDL
                     AQGFGIARPMPAASLPQWMAEFRPDELWSAASAFTWAREDLPMLIAEVDHRRWMKALE
                     NALAVAPDSAPTIELDHLACGFGRWYQGPGRKRYGEMPTFAAIDPIHQYLHSLARQMF
                     EQRQAGCEESLAESWQALYAASREMSELLQQLQAEILIGSQTSKR"
     gene            complement(5907..>6096)
                     /locus_tag="PLN02_01515"
     CDS             complement(5907..>6096)
                     /locus_tag="PLN02_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HOI51026.1"
                     /translation="LPESALQDPYGNDMISAIPFLAPEPSRRVALAWRSCFVRPKAID
                     ALLAAVRGLDSPAYRLLV"
BASE COUNT          986 a         2052 c         2022 g         1036 t
ORIGIN      
        1 cggtcaccgc ggcatgggcg gcgatcgcag cgccgacggt gccggcggcg gccttgccga
       61 tggtgtcggc gttggattcg gcgccgaacg gcgtgatcga ggtgacccgg tcgtagaaac
      121 tgcccttgtc ccagaagccc tcttccgagc agccgatgca gccgtggccg gactgcaccg
      181 gccagctgac gccaccgttc cagcgcatcg acgagcaggc gttgtaggtg gtcgggccct
      241 tgcagcccat cttgtacagg cagtagccct tgcgcgaacc ttcgtcgtcc caggcttcgg
      301 caaactggcc ggcgtcgaag tggccgcggc ggtagcactt gtcgtggatg cgctggccgt
      361 agaacatctt cggccggccc tggcgatcga gctcgggaat gcggtcgaag gtcagcatgt
      421 aggtgacgac gccggtcatc acttcggcga tcggcgggca gccgggcacg ttgatgatcg
      481 gcttgccgga aatcaccttg tgcaccggcg tggcgcgcgt cgggttcggc ttggcggcct
      541 gcacgcaacc gtacgaggcg caggcgcccc agctgatgat ggccttggcg tcggcgcagg
      601 tttccttgag cacttcgacg aagggccggc cgccgtggat gcagaacatg ccgtcctcgt
      661 tgagcggcgg gttgccctcg acggcgacga tgtaattgcc tttgtacttc ttcttgacct
      721 cttcgatgat cgcctcggcc tggtggccgg cggcggccat cagtgtgtcg tcgtagtcga
      781 gcgagagcat cgacagcacg acgtccttgg tcagcggatg cgccgagcgg atgaaggact
      841 cggagcagca ggtgcattcc aggccatgca gccagatcac cggcgtgcgt ggcttggttt
      901 ccatcgcctg ggcgatgcgc ggcgcggcgg cactgcccag cccgagcgag gtcgcggtca
      961 ggctgcagaa tttcaggaag ctgcgccggg tgattccctg gcgacgcagc acttcataga
     1021 atgtttcagt cactttgctc cccccgatgg tcagtcttgc ggtggtgagc ttccgcaaag
     1081 gtcgtgccac acccggtgtt tcgccgatta gtcttgaaaa ccaagggttt ggctggaagc
     1141 gcccggtgcc ggtgccggcc cttgcgaagg tggtgatgaa agcttgatta ccgcttatgg
     1201 aaactaattt tgcattgctt gccaatcgcc gaaaattgcc gtgcatgccc cgagaatctt
     1261 gcactcgatc aacacaagcc ggtcgcgcct gaatagaatc cggggatgcc gaccgtagcc
     1321 gacctgatga ccctgcacgc cgacagcgtg gccccttccg caacacttgc cgaagcggcg
     1381 gcattgatgg tcgaggcgag gatttcctcg gtggtcgtcg tcgacgcgac gcgggtggtc
     1441 ggcatcctga ccgagcgcga catgctgcat gcgatgcgcc gacacgagga tcagcggcgg
     1501 ccggtggcgc acttcatgac tgcgccggtg catacggtga gccacgatac cgaggtgcgc
     1561 agcgccttcc gccaggcgac gcggctgggt atccgccacc tggtggtgac gggcggcgaa
     1621 ggccgtctgc tcggcatcgt cagcgagacc gatttccgcc gcttcttcgg gctcgatttc
     1681 tatcgccagc tcaataccgt cgaaggcctg atggaccggg ttttcccccg cctgccgcgg
     1741 caggcgacac tggatgaggc gctggcggcg atggagacgg cgcgggcgac cagcgtcgtc
     1801 gtcgtcgacg ccggtcagcc ctgcggtatc gtcaccgagc gcgatgtcgt ccgcctctac
     1861 ctgcaggctt ccggcaatcc gacgctgggc gaggtgatga cgcagccgct gaccagcgtc
     1921 ctgcccgaga cctcggccgc cgaggcggcg gcgctgatgc tggaaagccg catccggcat
     1981 ctcgtcgtgg tcgatgccga aggccgcttg ctcggcctgc tcaccgagca cagcctgatc
     2041 cggccgctgg aaatggatct gctcgacgag acgatcagcg agcagcgcgg gttgcagacc
     2101 cgctacgacg aagcgctgct gatccgcaac gcggcgatga ccggcatgct gcgcggcgaa
     2161 cggctcgccg gcctgctcga actgatcgcc ctctccgccg agcacgaggt ccccggcatc
     2221 atctgttcga tcatgctggc cgacgccgac ggcagccacc tgcacgtcgt cgcggcgccc
     2281 ggcctggccg aggacgcacg cgccctgctg gcgaacatgc cggttgccga aggggtcggc
     2341 gtttccgggg cggcggcctg gaggcgcggc gaagcctata tcgagaacat tcacgaaagc
     2401 agcgccggca tcgactatcg tggtttcgcc cgccggatcg gtatcacctc cggctgggcg
     2461 gaagccttgc tcggtcccga cggccgcctg ctcggcacct ttgccgtcta tcgcgagcag
     2521 gccggtcgcc tgcccgacgg cagccgcgaa accatccgcc aggccagccg tctcgctgcc
     2581 ttgatgatcg cccaccaaca gcaggccgat gagctcgggc gcagccagca gaccttccgg
     2641 gagttgttcg aatcggtggg cgacgcgctc ttcgtcatcg acggcgacgg tcgctgcctc
     2701 gacgccaacg cggcggccga gcggatgacc ggctaccggc gcgaggaact gcgtggtcat
     2761 gcgccggact tcctggctgc ccagggcttg aacgacctgg cccggatcgc cgcggtgatc
     2821 cgctcggcta ccggcggtgc gccacaggaa ctggagttct gggggtgcga ccgcgacggc
     2881 cgggtgctgc tggtgcgcct gcagatttcc gccacgcagt acgctggcaa gccggcgctg
     2941 atcgtcaccg cgatcgacat cagcgtcacc cgcgccgagc agttgcgctt gggcatcgag
     3001 cgcgatctgg ccaacgccct gaataccgag gccgaccccg aggcgctact ggcgaccatg
     3061 ctcgacctcg ccttgcgcta cccggaattc gacagcggcg ggttctactg gcagaacgcc
     3121 ggcggcgact acgagctgcg cgtgcacaag ggcctgtcgc ccgcgttcgt cgccgctgcc
     3181 cgttgctgcg tggcggattc tccgaaggca gaactgatcc gcaacggcaa ggtgctgtgc
     3241 agttgtcaca cctgctgtca gcagcaggat cactgcagtc tgatcgatcc cgcgcactac
     3301 gccggcgaag gcctgcgctg cctgatcgcc ctgcccatcg tcgtcaacgg tcgggcgatc
     3361 gcctgcctca acctggctgg acatcgtact gtgcagatct cggtcgccac ctgccaggcg
     3421 gtgcaacacc tggtcagtca gtacgcgatg gcgctcgaac gcatcgaagc ccgcgaggcg
     3481 gcgcggcagt cgcgggacaa tctgagcggc ctgttcgcct cgctcgacga cctgatcttc
     3541 gtcatcgacg ccggtggccg catcgtccat tacaacccgg tggtcgacca ggcgctcggc
     3601 tacgcgccgg gcatcctgca cggcgaaccg gtctcccggc tttacccggc gtcctggcgc
     3661 gagcaggcgc agcacaatct cgacgagatg ttcgccggtc gcctgaatgc ctgtccggcc
     3721 cccttgctgg ctgccgacgg tcgtctggtg cagaccgagg tccgcgccgt gcacgggcac
     3781 tggaacggcg aggcagcgct gatcgccgtc gctcgcgacg tcggcgaacg gctggcggcc
     3841 gaagagcggc tgcgcctggc cgccagcgtc ttcgccaacg cccacgaagg catcatgatc
     3901 accgatgttt ccgggcacat cgtcgaagtg aacccgacct tcagcgaact gaccggctac
     3961 agtcgcgccg aggcgatcgg ccagaacgcc ggcatgctca attccggcca tcatgacgcc
     4021 gccttctacc gcaacatgtg ggaagcgatc cgcgatcaag gccattggcg cggcgaagtc
     4081 tggaaccgca agaaatccgg cgagatcttc gtcgagttgc tgaccatctc ggcggtgcgc
     4141 gacgccgagc ggacgatcac ccatttcgtt ggcatctttt ccgacatcac gctgctcaag
     4201 gagcaccagc agcgtctcga acgtctcgcc catttcgacg ccctgaccca gttgcccaac
     4261 cgcatgctgc tcgccgaccg gctgcaactg gcgatggcca acgccgcccg caatcgcggc
     4321 ctgctggcgg tctgctatct cgacctcgac ggtttcaagc cggtcaacga ccgcttcggc
     4381 catgccgccg gcgaccgttt gctggtcgaa gtcgcccagc gtctgcgcaa ctgcgtgcgc
     4441 ggcggcgaca ccgtcgcccg cctgggcggc gacgagttcg tgctgctgct gtccacgctc
     4501 aacgacctca acgagtgcga tcaggcgctg agccggattt ccgcgtcgtt gacccagcct
     4561 ttcgcggtgg ccggcgagaa cgtgacgatt tcggccagca tcggcgtcac cctttacccg
     4621 cacgacggtt ccgatgccga caccctgctc cgccacgccg accaggcgat gtacgtggcg
     4681 aagcaggccg ggcgcaaccg ccaccacctg ttcgatcccg agaaagaccg ccgggcgcgc
     4741 cgtcgccgtg aggacatcac tcgcatcggc cagggcctgg cggccggcga gtttgtcctc
     4801 cattaccagc ccaaggtcga catgcggcag ggccgtgtgg tcggcgccga agccctgatc
     4861 cgctggcagc atccgcaacg cggcctgctg ctgccgcagg agttcctgcc ggcgatcgag
     4921 ggcaacgagc tggcgatcga cctcggcaac tgggtcatca ccacggcgct cgaccagttg
     4981 tcgagttggg cggcggccgg tatcgacctc aagctgagca tcaacatcgc cggcgaacac
     5041 ctgcagcatc ccggcttcgc cgccgaactc gggcggcaat tggcggcgca cccggaactc
     5101 gatccgtcac ggctcgaact ggaggtgctg gaaaccgcgg cgatcgagga tgtcggccgc
     5161 gtcgcccaac tgtttacgga atgtcgccag ctcggcgtca gcttcgcgct cgacgatttc
     5221 ggcaccggtt attcctcgct cacctatttc cgccgcctgc cggcggaaat cctgaagatc
     5281 gaccagtcct tcgtgcgcga catgctcgac gacccggaag acctcgccat cgtcgaaggg
     5341 gtgatcggcc tgacccgcgc cttccgccgc caggtgatcg ccgagggcgt cgaatcgatc
     5401 gagcacggcc tcgtcctgct gctcctcggc tgcgacctgg cgcagggctt cggcatcgcc
     5461 cggccgatgc cggcggcgag cctgccgcag tggatggcgg aattccgccc ggatgagtta
     5521 tggtcggcgg cctcggcctt cacctgggcg cgcgaggacc tgccgatgct gatcgccgaa
     5581 gtcgatcacc ggcgctggat gaaggccctg gagaacgcgc tcgcggttgc gccggacagt
     5641 gcgccgacca tcgagctcga tcacctggcc tgcggcttcg ggcgctggta ccaggggccg
     5701 ggccgcaagc gctacggcga aatgccgacc ttcgcggcga tcgatccgat ccatcagtat
     5761 ctgcattccc tcgcccggca aatgttcgag cagcggcagg ccggctgcga ggaatcgctg
     5821 gccgagtcct ggcaggcgct atatgcggcc agtcgggaaa tgagcgagct gctgcagcaa
     5881 ctgcaggccg aaatcctgat cggcagtcag accagcaagc ggtaggccgg cgaatcgagg
     5941 ccacgcacgg cggcgagcaa ggcgtcgatc gccttcggtc ggacgaagca actgcgccag
     6001 gccagcgcca cccggcgcga aggttccggg gcgaggaagg gaatggcgct gatcatgtcg
     6061 ttgccgtagg ggtcctgcag cgcactttcc ggcagc
//
LOCUS       DAOSDX010000040         6077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4665_length_6077_cov_3.686483, whole genome shotgun sequence.
ACCESSION   DAOSDX010000040 DAOSDX010000000
VERSION     DAOSDX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4665_length_6077_cov_3.686483"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /gene="tssK"
                     /locus_tag="PLN02_01520"
     CDS             complement(<1..385)
                     /gene="tssK"
                     /locus_tag="PLN02_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HOI51027.1"
                     /translation="MNGAQLLWGEGLFLRPQHFQLQDLHWNTTARQVLLSANPYAWGI
                     RELEIDRDALKNGVLRFNRVDVLFANGDSLIAPEIDQLPPPLALADAVTEDGNVEFYL
                     ALHHLNPHGGNTAENQENASQARYLS"
     gene            complement(401..1033)
                     /gene="tssJ"
                     /locus_tag="PLN02_01525"
     CDS             complement(401..1033)
                     /gene="tssJ"
                     /locus_tag="PLN02_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HOI51028.1"
                     /translation="MFFDIKYESHLSKKPRVGALLAAALLATNLTGCATSGVQVAGAA
                     IGAVLEAGGLIKRDGRPRDIAVKLVADKTLNQTSEGVPMSLVAKIYVLRSPERLRSLS
                     YTELSESTSEATIFGDDLISTREVVLIPGKSYDLRLKIPSDARTLAIVGMYRSPSPNR
                     WRIALDAQESVSDGIIATAHACALSLKEGILVNDISAESVRSLPEMRCNG"
     gene            1162..1794
                     /locus_tag="PLN02_01530"
     CDS             1162..1794
                     /locus_tag="PLN02_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI51029.1"
                     /translation="MTSPARLIFSVTLCATVFFGGCATAPTASSDEQFSELMMQTEIR
                     ASESIENGNKDDAVRIVSELVAANPGRKEPWIRIAKIHFDATDYAEAIVAADEALKID
                     NNDRTAKSIRAVSGLRVAAQSLADLRNDAELKGNARADAAGLAMTMRDILGEDVLVPP
                     AELEARKRREARARREAAIASRQRNVRAEPQQPARETQATATGNPFGNLK"
     gene            1864..2367
                     /gene="tssB"
                     /locus_tag="PLN02_01535"
     CDS             1864..2367
                     /gene="tssB"
                     /locus_tag="PLN02_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HOI51030.1"
                     /translation="MAKKESVQKRLQKVRPPRVQLTYDVEIGDAVEVKEIPFVVGVLG
                     DFAAQSKTPQGRVRDRKFVNIDLDNFDDVMTGMAPRAAYRVKNRLSPEGGEIGVDLEF
                     QKFEDFRPEAVVQQVEPLRKLQEARSKLADLRNKLAGNEKLEDLLSDVLSNTEQLHEL
                     DNDGGEK"
     gene            2370..3845
                     /gene="tssC"
                     /locus_tag="PLN02_01540"
     CDS             2370..3845
                     /gene="tssC"
                     /locus_tag="PLN02_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HOI51031.1"
                     /translation="MQQAVAQPAAQVQPGILDQIIAESRVAQSDQERHRARDIISELV
                     RQVLQGEVVVSENLAASLDARVAELDRLISEQLSEVMHAPEFQALESAWTGLHYLCKQ
                     TSTGEMMKIKLLNVGKRELVKDFKTAIDFDQSALFKKVYEEEFGTFGGAPFGALVGQY
                     ELNRQAEDMYFIEQMSHIAAAAHAPFVAAASPQLFGLDSFTDLSKPRDMSKVFDTVEY
                     AKWKSFRESEDSRYVGLALPRFLGRLPFNPIDGEVTEGFNFVEEVDGTNHDKYLWCNA
                     AFAFAARLTSAFENYGWCAAIRGVEGGGLVEDLPTHTFRTDDGEVALKCPTEVSITDR
                     REKELSDLGFIPLVHCKNTDYAAFFGAQSTQRPRKYDTDSANANASLSAQLQYMFAVS
                     RIAHYMKAMMRDKIGSFASAANVQNYLQRWVDQYVTADDSASQETKAQFPLREASIEV
                     AEVPGRPGVYRAVAFIRPHFQLDELSVSLRLVAELPQSGKA"
     gene            3882..4418
                     /locus_tag="PLN02_01545"
     CDS             3882..4418
                     /locus_tag="PLN02_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HOI51032.1"
                     /translation="MKDIYVEFKGSDIKGDSRDARHKDQVEVSTWSHTMRQPKSATAS
                     GAGGHTAERVEHGEMFFTKDIDGSSPKLYQACSSGLVVNDVVIYFYRAFGGKNTTGTP
                     SGTQNRHQYMKIELKNVIIASVSPSVDSEDIPSETFSLKYSAIKWTYDELNIDGNKSG
                     KVNIQGAWNLATNTPSLT"
     gene            4438..4944
                     /gene="tssE"
                     /locus_tag="PLN02_01550"
     CDS             4438..4944
                     /gene="tssE"
                     /locus_tag="PLN02_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HOI51033.1"
                     /translation="MAELVSAFFMNKGFEPSLFDKLFDDRPVAAARRRLSIEQLKDTV
                     ARDLESLLNTRSLIDETQSADFPLSRRSVAAFGMGDFAGLSLASVYDRKRICSAIEQA
                     ISAHEPRLRDVRVTLELHRKTINALYFSITAVLQVKPAQEPVAFDALLQPTSLQYSVQ
                     RQKPRPGM"
     gene            4945..>6077
                     /gene="tssF"
                     /locus_tag="PLN02_01555"
     CDS             4945..>6077
                     /gene="tssF"
                     /locus_tag="PLN02_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HOI51034.1"
                     /translation="MEDLLPYYERELAFLRQHSRQFAERYPKLAAQLLLSGEGCDDPH
                     VERMIESFALLTARVSKKLEDSYPQFTEALLNVLYPHYLRPFPSCSIAHFDDTGNTDT
                     TTIVPRHTELHSRPIRGAVCKFRTAWALKLAAVDLDQLVFNPIASAPSDIRLPVGSNA
                     LFSFSLGVRGHHGDEGFQRIGKLRFYIDAEPSVASALRDALFLRTLKVYAAGADERWH
                     AVDRGMIGEVGFAADEALIEMPDTAHDAYRYLTEYFCFPEKFNFFELDLGKLPARLRG
                     ERKVRLHVVFGNLRADSDASRLMQTLTTKNLRLGCVPVVNLFPQRGDPIRVSHRLSSY
                     PVVADGRRAYAYEVYAIDSVRRVRQTPQGEAITEFRPFFALRHG"
BASE COUNT         1263 a         1782 c         1788 g         1244 t
ORIGIN      
        1 gcgagaggta acgggcctgg ctagcgtttt cctgattctc ggcggtattg ccgccatgcg
       61 gattgagatg atgcagggcc aggtaaaatt caacgtttcc atcttcggtc accgcatcgg
      121 cgagcgccag cgggggcggt agctgatcga tttctggagc gatcagcgaa tcgccgttgg
      181 cgaacaacac gtcgacacga ttgaagcgca gaacgccgtt cttcaaggcg tcgcgatcaa
      241 tctccaactc ccggatgccc caggcgtagg ggttcgcgga aagcagcacc tgtcttgccg
      301 tggtattcca gtgcaggtcc tgcaactgaa aatgttgggg gcgaaggaat aatccctcac
      361 cccagagcaa ctgtgctccg ttcattccat atccttcagg tcagccattg cagcgcattt
      421 cgggcagcga gcgaaccgat tcggcgctga tgtcgttgac gagaattccc tccttcaaac
      481 tcagggcgca agcatgtgcg gtggcgatga tgccgtcgga aaccgattcc tgcgcgtcta
      541 gggcaattcg ccagcggttc ggggagggcg agcgatacat gccgacgatg gccagggttc
      601 tggcatcgct gggtatctta agacgaaggt cataagattt tcccggaatc agtaccactt
      661 cgcgcgtcga gatgagatcg tcgccgaaaa tggttgcctc cgatgtgctt tccgataatt
      721 cggtgtagct gaggcttctc aatcgctcag gggagcgcag gacgtaaatc ttggccacca
      781 gtgacattgg aacgccttcg ctggtctggt tgagcgtctt gtcggcgacc agttttaccg
      841 cgatgtcacg cgggcgaccg tcgcgcttga tcaatccgcc ggcttcgagg acggcgccaa
      901 ttgcggcgcc ggcgacctga acgccggagg tggcgcaacc ggtcaggttt gtagccagga
      961 gtgcggccgc gaggagagcg ccgactcttg gcttcttcga tagatgcgat tcatatttga
     1021 tatcaaaaaa caacgatggc atatatgcat acgtgttgat ttttccggag gcgttaatgt
     1081 actatgcctc cttgtttcag gcaaacccat cggcattact tgtcatgaca aatacaaagc
     1141 cggctttcag atggagcgac catgaccagc cccgcaaggc tcattttttc tgtaaccctc
     1201 tgtgccacag tcttttttgg tggatgcgcg acagccccga ccgcttccag cgacgagcag
     1261 ttttccgagt tgatgatgca gaccgagatt cgggcttctg aatcgatcga gaacggtaac
     1321 aaggacgacg ccgttcgcat cgtcagcgag ttggtggctg ccaatccagg gcgcaaggaa
     1381 ccgtggatca ggattgccaa gattcatttc gacgcgaccg actatgccga agccatcgtc
     1441 gctgccgacg aagccctgaa gatcgataac aacgaccgta ccgcaaagag catccgtgcg
     1501 gtatccgggc tgcgggttgc cgcgcagtcg ctggccgatt tgcgcaatga cgccgaactc
     1561 aagggcaatg cccgcgccga tgcagccggt ctggcgatga ccatgcgcga cattcttggc
     1621 gaagacgtcc tggttccccc agctgagctc gaagccagaa agcgtcgtga agcccgggcc
     1681 agaagagaag cagcgattgc ttcgcgtcag aggaacgttc gggccgagcc gcaacaaccg
     1741 gccagagaaa cgcaggcgac cgcgactggc aatccttttg ggaatctgaa gtagagggta
     1801 ggggggcgcc ggtcgccacg gaccggtaag ccgaggcatt tttcgatcca tcgggagaaa
     1861 tttgtggcaa agaaagaaag cgtccagaag cgtctgcaaa aggtcaggcc gccgcgtgta
     1921 caactcactt acgatgtcga aatcggcgac gccgtcgagg tcaaggagat tcccttcgtt
     1981 gttggggtgc tgggcgattt tgccgctcag agcaagaccc cgcaggggcg ggtgcgcgac
     2041 cgcaagttcg tcaatatcga tctcgacaac ttcgatgacg tgatgactgg catggcaccg
     2101 cgggcggcct atcgggtcaa gaatcgtctg tccccggaag gcggggagat tggtgtcgat
     2161 ctcgaattcc agaagttcga ggactttcgt ccagaagctg tagtgcagca ggttgagccc
     2221 ctgcgcaagt tgcaggaagc acgctccaag ttggccgatc tgcgcaacaa gctggccggc
     2281 aacgagaaac tcgaggatct gctcagcgac gtcctgagca ataccgaaca actgcacgaa
     2341 ctggacaacg acgggggcga aaaatgagca tgcagcaggc agtagcccaa ccggccgccc
     2401 aggtgcagcc gggaattctc gatcagatca tcgccgaaag tcgtgtcgcc caatccgacc
     2461 aggagcgtca tcgggcccgc gacatcatca gcgaactggt tcgccaggtc ttgcagggcg
     2521 aagtggtcgt ctcggaaaac ctcgccgcgt cgctcgacgc gcgggttgcc gaacttgatc
     2581 ggctgatttc cgaacagctc agcgaggtca tgcacgcgcc ggaattccag gcactggaaa
     2641 gcgcctggac cggcttgcac tacctgtgca agcaaacctc gaccggggag atgatgaaaa
     2701 tcaaacttct caacgtcggc aagcgggaac tggtcaaaga tttcaagacg gcgatcgact
     2761 tcgatcagag cgcgttgttc aagaaggtct acgaagaaga attcggtact ttcggcggtg
     2821 ctccttttgg cgccctggta ggtcaatacg aactgaatcg ccaggccgag gacatgtact
     2881 tcatcgagca gatgtcgcat atcgcggcgg cagctcacgc gccttttgtc gcggccgcct
     2941 cgccgcagct ctttggtctg gacagtttca ccgacctgag caagccgcgc gacatgtcga
     3001 aggtattcga tactgtcgaa tacgccaaat ggaagtcgtt ccgggaatcc gaggattcgc
     3061 gttatgtcgg gctggcgctg ccgcgcttcc tcgggcgcct gccgttcaat ccgatcgacg
     3121 gtgaagtgac cgagggcttc aacttcgtcg aggaagtcga tggcacgaat cacgacaagt
     3181 acctctggtg caatgccgcc tttgcctttg ccgcaagatt gacctcggct ttcgaaaact
     3241 acggctggtg cgcagccatc cgtggtgtcg aaggcggtgg cctggtcgag gatctgccga
     3301 cgcacacctt ccggactgac gatggcgagg tcgcgctgaa gtgcccgacc gaggtttcga
     3361 tcaccgaccg ccgggaaaag gagttgagtg atctcgggtt cattccgctg gtgcattgca
     3421 agaataccga ttacgcggcc ttcttcgggg ctcagtcgac gcagagaccc agaaagtacg
     3481 ataccgactc tgccaacgcc aatgcttcgc tctcggcaca gttgcagtac atgtttgcgg
     3541 tttcacgcat cgcccactac atgaaggcaa tgatgcgcga caagattggt agcttcgcct
     3601 cagcggcgaa cgtgcagaac tatctgcagc gctgggtcga tcagtacgtg accgcggacg
     3661 attccgcgtc gcaggaaacc aaggcgcaat ttccgttgcg cgaagcttcg atcgaggttg
     3721 cagaagttcc gggtcgcccc ggcgtctacc gggcagtagc gttcatcagg ccgcatttcc
     3781 agcttgatga gctttccgtt tcattgcgtt tggtcgccga gttgccgcag tctggcaagg
     3841 cctgatcttg tctttgtttt taaccaagga gaaagtagga aatgaaggat atatacgttg
     3901 agttcaaggg tagcgacatc aagggggatt cccgcgatgc ccgacacaag gatcaggttg
     3961 aagtttcgac ctggagccac acgatgcgcc agccgaagtc ggcgaccgcc tcgggtgccg
     4021 gcggccatac tgccgagcgc gtcgagcacg gcgaaatgtt cttcaccaag gacatcgacg
     4081 gttccagccc gaagctctac caggcctgct cgtccggcct cgtcgtcaat gacgtggtga
     4141 tctacttcta ccgcgccttc ggcggcaaga acacgaccgg caccccgtcg ggtacgcaga
     4201 accgccatca gtacatgaag atcgaactga agaacgtgat catcgcgtcc gtttcgccgt
     4261 cggtcgatag cgaggacatt ccgtccgaaa ccttctcgct caagtattcg gcgatcaagt
     4321 ggacctacga cgaactgaac atcgacggca acaagtccgg caaggtcaat atccagggtg
     4381 cctggaacct cgctaccaac acgccgagcc tgacctgatc cctgattcgt gcacgaaatg
     4441 gcggaactcg tttccgcctt ttttatgaac aaaggtttcg agccgtccct gtttgacaaa
     4501 ttgttcgacg accgacctgt cgccgcggcg cggcggcggc tgtcgatcga gcagttgaag
     4561 gacacggtcg ctcgcgatct cgaatcattg ctcaataccc gcagcctgat cgacgagacg
     4621 cagagcgccg actttccgct gtcccgtcgt tcggtcgccg cgttcggcat gggcgatttc
     4681 gccggcctta gcctggccag cgtctatgac cgcaagcgca tctgcagcgc cattgagcag
     4741 gcgatttccg cgcacgaacc gcgtctgcgg gatgtccggg tgactctgga gctgcatcgc
     4801 aagacgatca atgcgctgta cttttcgatc actgccgtgc ttcaggtgaa accggcacag
     4861 gagccggtcg ccttcgatgc cctgttgcaa ccgaccagtc tgcagtattc ggttcaacgt
     4921 cagaagcccc gcccaggaat gtaattggaa gacctgcttc cctactacga acgagaactg
     4981 gcatttctcc gccagcattc ccggcagttt gccgagcgtt atccgaagct ggcggcacag
     5041 ttgctgctgt ccggagaagg ctgcgatgac ccgcacgtcg aacggatgat cgaatccttt
     5101 gcgctgctga ccgcgcgcgt ctcgaaaaaa ctagaggaca gctatccgca gtttaccgag
     5161 gccctgttga atgttctcta tccgcactat ctgcgcccct tcccgagttg ttcgatcgcc
     5221 catttcgacg ataccggcaa caccgatacg acaaccatcg ttccccgcca taccgaactg
     5281 cattcgcggc caatccgcgg tgccgtctgc aagttccgca ccgcttgggc gctcaagctg
     5341 gcggcggtcg acctcgacca gctcgtcttc aatccgattg ccagcgcacc gagcgacatt
     5401 cggctgccgg ttggcagcaa cgcgcttttt tccttctcgc tcggcgtgcg cggacaccat
     5461 ggcgacgagg gtttccagcg catcggcaag ctgcgtttct acatcgatgc tgaaccatcg
     5521 gtcgcttcgg cactgcgcga cgctctgttc ctgcgcacgc tgaaagtcta tgcggccggt
     5581 gccgacgagc gctggcatgc cgtcgaccgg ggaatgatcg gcgaagtcgg tttcgccgcc
     5641 gacgaggcac tgatcgagat gcccgacacc gcacacgacg cctatcgcta tctcaccgag
     5701 tatttctgct ttcccgagaa attcaatttt ttcgaactcg atcttggcaa attgccagcg
     5761 cgcctgcgcg gcgagcgcaa ggtccgcctg cacgtcgttt tcggcaacct gcgcgcggac
     5821 agcgatgcct cgcgcctgat gcagacgctg accacgaaga acctgcgcct gggctgcgtg
     5881 ccggtggtca atctgtttcc ccaacgtggc gatccgatcc gcgtcagcca tcgactgtcg
     5941 tcctacccgg tcgttgccga cgggcgccgc gcttacgcct acgaagtcta tgccatcgac
     6001 tcggtacgcc gtgtccggca gacgccgcag ggtgaggcca tcaccgagtt ccgtcccttc
     6061 tttgccctgc gtcacgg
//
LOCUS       DAOSDX010000041         6045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4696_length_6045_cov_3.201836, whole genome shotgun sequence.
ACCESSION   DAOSDX010000041 DAOSDX010000000
VERSION     DAOSDX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4696_length_6045_cov_3.201836"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="PLN02_01560"
     CDS             <1..1657
                     /locus_tag="PLN02_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI51035.1"
                     /translation="FTHATEGMVITDASSRIVAVNPAFCSITGYAPEEVIGKTPALLN
                     SGRQGEDFYRNMWQSLIQIGQWQGEIWNRRKDGGIYPEWLSIAAVPDEPGESGHFIGV
                     FTDISERKQSEARIHHLAHHDALTGLPNRLLLEDRIEQGMLKSKRMHRQMGIIFIDLD
                     RFKNINDTLGHAVGDELLIQAAQRGLNALRETDTLCRQGGDEFVVVLPELEGGQDAAH
                     ICSKLLSAFCQPYLLAGHELTVSGSAGIALYPEDGESASELLRKADAAMYRAKEEGRN
                     TFCFFSPEISTATLGDLLLENDLFGALDRQELLMYYQPKVDAGSGEVVGAEALIRWQH
                     HERGLVSPEQFIRLAEENGLINAIGEWAIRNVCAQQRAWLDAGLLVVPVAVNISALQF
                     AQQDLPNMLAHVLADYGLPPQLIELELTESLLMRNSARAALVLDRLRKMHVQVALDDF
                     GTGYSSLSYLRQFPLHALKIDRSFVSQIDENGEPVRLASAIIAMAHELDLVVIAEGVE
                     NEVQANYLAAHGCDHFQGYFYGRPQAVEDFGKLLAGRRLGSVA"
     gene            complement(1622..2686)
                     /locus_tag="PLN02_01565"
     CDS             complement(1622..2686)
                     /locus_tag="PLN02_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOI51036.1"
                     /translation="MHLDDFRARLRALGAKPAHEARLLRAWLQGSSLDAGSRRQAAEN
                     YLPLALRQALPELEEELAGLARVVSEHPASDGSRLLVELADGQSVESVLLPRDGVCIS
                     TQIGCAVGCTFCMTGRSGLLRQVSSAEMLAQVVLARRRQSLRRVVFMGMGEPAHNLDN
                     VLETVDLLGTAGGFGHKNLVFSTVGDPRVFERLPQQKVRPALALSLHSTDAALRARLL
                     PRAPQMTPELLVALAEDYARLTGYPIQYQWTLLAGINDSLAEMDGVVRLLRGKYALIN
                     LIPYNTTAALDFQRPSSERITELAHYLHAHGIRTTVRQSAGQEVDGGCGQLRARRLDG
                     SLASGRRAGSGNAAKPAAGQ"
     gene            complement(2698..3390)
                     /locus_tag="PLN02_01570"
     CDS             complement(2698..3390)
                     /locus_tag="PLN02_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOI51037.1"
                     /translation="MTRDQFSKIAYNGLWKQNTALAQLLGLCPLLAVSTNIVNGVMLA
                     LATILVMAMSNLAIAALRNLIPHEIRIPVFILIVAALVTVVDLLFNAQLHELYVVLGI
                     FIPLIVTNCIVLARVEAFAAKNPPLDSTIDGIFMGFGLLWVLALLGGLRELLGNGMLF
                     GGIDMVFPALSPIRILPESYPGFLLALLPPGAFILLGCLIAWKNWMDARAAERARRKP
                     PVAGEATAEAGA"
     gene            complement(3387..4052)
                     /gene="rsxG"
                     /locus_tag="PLN02_01575"
     CDS             complement(3387..4052)
                     /gene="rsxG"
                     /locus_tag="PLN02_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HOI51038.1"
                     /translation="MTSTPREFTASGMALRTAAILFLFVIVFTGLLSAAYQWTKPSIE
                     ASALAEKMKLLNEVLPAADYDNNLLEDSLSLPPTPELGLKEASTLYRARKAGQPAALI
                     FEAAAPDGYAGRIRLLIAVRADGKVAGVRVTQHKETPGLGDYIDPRKDKNKAAPWIDQ
                     FVGLSLASAGDKAWRVRKDGGRLDYHAGATVSPRAVAKAVQRAVRWVDGERDRLFSEG
                     KQP"
     gene            complement(4049..5071)
                     /locus_tag="PLN02_01580"
     CDS             complement(4049..5071)
                     /locus_tag="PLN02_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOI51039.1"
                     /translation="MMFAPAPFLLKDASVSQVMTQVCLALIPGIAVYAWLVGPAILVQ
                     LAIASFTALLAEAAILRLRDKPLALFLSDGSAVVTAWLIALTFPPLAPWWLVATGTLF
                     AIVVAKHLYGGLGQNPFNPAMVAFAVCIVAFPALMSQWPSIGLKLPLLDQLNIIFGLA
                     PRVDALSGATPLDAMKTALKLGEGQVNVAHLLENQDIYGNFAGRGWEWVTGAYLLGGL
                     WLWQRKLITWHAPLAFLAAMVLISGGLWLYRPESFASPLFHLLSGGAMLGAFFIITDP
                     VSGCTTPRGKLIFGFGAGLLAYIVRVFGGYPDGVAFAVLLMNLSAPLIDRLTQPRIFG
                     MKEGRP"
     gene            complement(5068..>6045)
                     /gene="rsxC"
                     /locus_tag="PLN02_01585"
     CDS             complement(5068..>6045)
                     /gene="rsxC"
                     /locus_tag="PLN02_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOI51040.1"
                     /translation="IRVLTGKEVPGAMRSTADMGVQVFNVGTAYAAWRAIGHGEPLIS
                     RVVTLTGNVRQAHNVEALIGTPMDELLRLASPNPYTDGIIMGSPMMGFLVPDETAPIV
                     KTSNCLIAHSARLFPPKAPEMPCIRCGACARACPHELQPFELYWHSRAKDFGKTQQYH
                     IFDCIECGCCSFVCPSRIPLVQYFRFAKSEIWAREREKKASEEAKQRFEFKQFREERE
                     KADKAEKLAKAAAAQAAKKAAEAAAAAGGTVAAEAPAAAGDDPAAAAKKAAIAAAMAR
                     AKAQREAVQAKNTDELTPQQAHEMAAIEARRAAAGVSEAPAASTDSRTE"
BASE COUNT         1009 a         1924 c         2136 g          976 t
ORIGIN      
        1 gtttacccac gccaccgaag gcatggtgat caccgacgcc agctcccgca tcgtcgctgt
       61 caatccggcc ttctgctcga ttaccggtta cgcgcccgag gaagtcatcg gcaagacgcc
      121 ggccttgctc aattccgggc gccagggcga ggatttctac cgcaacatgt ggcagtcgct
      181 gatccagatc ggccaatggc agggcgagat ctggaaccgg cgcaaggacg gcggcatcta
      241 tccggagtgg ctgtcgatcg cggcagtgcc cgacgaaccc ggcgaatccg gccatttcat
      301 cggcgtcttc accgacattt cggaacgcaa gcagagcgag gcgcgcatcc atcacctggc
      361 gcaccacgac gcgctcaccg gcctgcccaa ccggctgctg ctggaggacc ggatcgaaca
      421 ggggatgctc aagtccaagc gcatgcaccg gcagatgggg atcatcttca tcgaccttga
      481 tcgcttcaag aacatcaacg ataccctcgg tcacgcggtc ggcgacgagc tgctgatcca
      541 ggcggcgcag cgtggactga acgcattgcg cgagaccgat acgctgtgcc ggcagggcgg
      601 cgacgaattc gtcgtcgtcc tgcccgagct ggaaggcggt caggacgcgg cgcacatctg
      661 cagcaagctg ctcagcgcct tctgtcagcc ctacctgttg gccgggcacg aactgacggt
      721 aagcggcagc gccggcatcg ccctctatcc cgaggacggc gagtccgctt ccgaactgct
      781 gcgcaaggcg gacgcggcga tgtaccgggc caaggaggaa gggcgcaata ccttctgctt
      841 cttctcgccg gaaatcagta ccgcgacgct cggcgacctg ctgctggaaa acgacctgtt
      901 cggcgccctc gaccgccagg aactgctgat gtactaccag cccaaggtcg atgccggcag
      961 cggcgaggtg gtcggcgccg aagcactgat ccgctggcaa catcacgaac gcggcctggt
     1021 ttcgccggaa cagttcatcc gcctggccga ggaaaacggg ctgatcaacg cgatcggcga
     1081 atgggcgatc cgcaacgtct gcgcccagca gcgtgcctgg ctcgacgccg gcctgctggt
     1141 ggtgccggtg gcagtcaata tttcggcgct gcagttcgcg cagcaggatc tgcccaacat
     1201 gctggcacac gtcctcgccg attacggcct gccgccgcaa ctgatcgagc ttgaactgac
     1261 cgaatccctg ctcatgcgca attcggcgcg cgccgcgctg gtcctcgacc gcttgcgcaa
     1321 gatgcacgtg caggtggcgc tcgacgattt cggcaccggc tattcttcgc tcagctacct
     1381 gcgccagttc ccgctgcatg cgctgaagat cgaccgcagc ttcgtcagcc agatcgacga
     1441 gaacggcgag ccggtccgcc tcgcttcagc gatcatcgcc atggcgcacg aactcgacct
     1501 ggtggtgatc gccgaaggtg tcgagaacga ggtgcaggcg aattacctgg cggcgcacgg
     1561 ctgcgaccat ttccagggtt atttctacgg ccgtccgcag gccgtcgagg atttcggcaa
     1621 gctactggcc ggccgccggc ttggcagcgt tgcctgatcc ggcgcggcgt ccgcttgcca
     1681 gcgagccgtc caggcggcgg gcgcgcagct ggccgcaacc gccatcgact tcctgcccgg
     1741 ccgactggcg gaccgtggtg cggatgccgt gggcgtgcag gtaatgcgcc agttcggtga
     1801 tgcgttcgct cgaagggcgc tggaaatcga gagcggccgt cgtgttgtac gggatcaggt
     1861 tgatcagcgc gtacttgccg cgcagcaggc ggacgacgcc gtccatttcg gcgaggctgt
     1921 cgttgattcc ggcgagcagc gtccattggt actggatcgg ataaccggtc aggcgggcgt
     1981 agtcctcggc cagcgccacc agcagttccg gcgtcatctg cggggcgcgc ggcagcaggc
     2041 gggcgcgcag ggcggcgtcg gtgctgtgca gcgacagcgc cagcgccggg cgcaccttct
     2101 gttgcggcag gcgctcgaag acgcgcggat cgccgaccgt cgaaaagacc aggttcttgt
     2161 gcccgaagcc gccggcggtg ccgagcaggt cgacggtttc gagcacattg tcgaggttgt
     2221 gcgccggctc gcccatgccc atgaagacga cccggcgcaa gctctggcga cggcgggcaa
     2281 ggaccacctg ggccagcatc tcggcgctgc tcacctggcg cagcaggccg ctgcgaccgg
     2341 tcatgcagaa agtgcagccg acggcgcagc cgatctgcgt cgagatgcag acgccgtcgc
     2401 gcggcagcag cacgctctcg acgctctggc cgtcggccag ctcgaccagc agtcggctgc
     2461 cgtcgctggc cggatgttcg gaaacgacgc gggcgagccc ggcgagttct tcctcaagtt
     2521 cgggcagcgc ctggcgcagc gccagcggca ggtaattttc cgccgcctgg cggcggctgc
     2581 cggcgtccag ggacgagccc tgcagccagg cgcgcagcag gcgtgcctcg tgggccggtt
     2641 tggcgccaag ggcgcgcagc cgggcgcgga aatcgtccag gtgcactgcg ctggctgtca
     2701 ggcgccggct tcggcggtcg cttcgccggc gaccggcggc ttgcgccggg cgcgttcggc
     2761 ggcgcgggca tccatccagt tcttccaggc gatcaggcag ccgagcagga tgaaggcgcc
     2821 cggcggcagc agagcgagca ggaagccggg ataactctcg ggcaggatgc ggatcggact
     2881 cagcgccggg aagaccatgt cgatgccgcc gaacagcatg ccgttaccga gcagctcgcg
     2941 caggccgccg agcagggcga gcacccagag caggccgaaa cccatgaaga tgccgtcgat
     3001 cgtcgagtcg agcggcgggt tcttggcggc gaaggcctcg acccgggcga gcacgatgca
     3061 gttggtgacg atcagcggaa tgaagatgcc cagcaccacg tagagttcgt gcaactgcgc
     3121 gttgaacagc aggtcgacga cggtgaccag cgcggcgacg atgaggatga agaccgggat
     3181 gcggatctca tgcggaatca ggttgcgcag cgcggcgatt gccagattcg acatggccat
     3241 caccaggatc gtcgccaacg ccagcatcac cccattgacg atgttggtac tgaccgccag
     3301 cagcgggcag aggccgagta gctgggcgag tgcggtgttc tgtttccaca ggccgttgta
     3361 agcgattttc gagaactggt cgcgggtcat ggttgcttcc cctccgaaaa cagccggtcg
     3421 cgttcgccgt ccacccagcg cacggcgcgc tgcaccgcct tggcgacggc gcgcgggctg
     3481 accgtggcgc ctgcatggta gtcgagacgg ccgccgtcct tgcgcacgcg ccaggccttg
     3541 tcgccagcgc tggcgagcga aagaccgacg aactggtcaa tccagggcgc cgccttgttt
     3601 ttgtccttgc gcgggtcgat gtaatcgccc aggcccggcg tttccttgtg ctgggtgacg
     3661 cgcaccccgg ccaccttgcc gtcggcgcga accgcgatca gcaggcggat acggccggcg
     3721 tagccgtccg gtgccgcagc ttcgaaaatc agcgcggcgg gctgacccgc cttgcgcgcc
     3781 cggtacagcg tgctggcttc cttcagcccc agttccgggg tcggcggcag cgacaggctg
     3841 tcctcgagca gattgttgtc gtaatcggcg gccggcagta cctcgttgag cagcttcatc
     3901 ttctcggcca gcgccgaggc ttcgatcgac ggcttggtcc actggtaggc ggcggagagc
     3961 aggccggtga acacgatgac gaagagaaag aggatggcgg cggtgcgcag tgccatgccg
     4021 gaggcggtga attcgcgcgg cgtgctggtc atggccgccc ctccttcatg ccgaagatgc
     4081 gtggctgggt caggcggtcg atcagcggtg ccgacagatt catcagcaat accgcgaagg
     4141 cgacgccgtc ggggtagccg ccgaagacac ggacgatgta ggcgagcagc ccggcgccga
     4201 agccgaagat cagcttgccg cgcggcgtcg tgcaaccgga gaccgggtcg gtgatgatga
     4261 agaaggcgcc gagcatcgca ccgccggaca gcaggtggaa cagcgggctg gcgaagctct
     4321 ccggccggta cagccagagg ccgccggaga tcagcaccat ggcggcgagg aaggcgagcg
     4381 gcgcgtgcca ggtgatgagc ttgcgctgcc acagccagag gccgccgagc aggtaggcgc
     4441 cggtcaccca ttcccagccg cggccggcga agttgccgta gatgtcctgg ttttccagca
     4501 ggtgggcgac gttgacctgg ccttcaccaa gtttcagtgc cgtcttcatc gcgtcgagcg
     4561 gcgttgcgcc ggacagggcg tcgacgcgcg gcgcgaggcc gaagatgatg ttgagctggt
     4621 cgagcagcgg cagcttgagg ccgatgctcg gccactgcga catcagtgcc gggaaggcga
     4681 cgatgcagac ggcgaaggcg accatcgccg gattgaacgg gttttgcccg agaccgccgt
     4741 agaggtgctt ggcgacgacg atggcgaaca gggtgccggt ggcgaccagc caccacggcg
     4801 ccagcggcgg gaaggtgagg gcgatcagcc aggcggtgac gaccgccgag ccgtcggaca
     4861 ggaagagcgc cagcggcttg tcgcgcagtc ggaggatggc ggcttcggcg agcaaggcgg
     4921 tgaacgaggc gatcgccagc tggaccagga tcgccgggcc gaccagccag gcatagacgg
     4981 caatgccggg gatcagggca aggcagacct gggtcatcac ctggctgacg ctggcgtcct
     5041 tgagcaggaa gggggcgggg gcgaacatca ttcggtcctg gaatcggtgc tggcggcagg
     5101 cgcttcggag acgccggcag cggcgcggcg ggcttcgatg gcggccattt catgcgcctg
     5161 ttgcggggtc agttcgtcgg tattcttcgc ctgcacggct tcccgctggg ctttggcgcg
     5221 cgccatcgcg gcggcgatgg cggccttctt ggccgccgcg gcggggtcgt cgccggccgc
     5281 tgccggcgct tcggcggcga ccgtgccgcc agccgccgcc gcggcttccg ccgccttctt
     5341 ggcggcctgg gcggcagccg ccttggccag cttctccgcc ttgtcggcct tttcgcgctc
     5401 ctcgcggaac tgcttgaatt cgaagcgctg cttggcttcc tctgaggcct tcttctcccg
     5461 ctcgcgggcc cagatttcgc tcttcgcgaa gcggaaatac tggaccagcg gaatccgcga
     5521 cggacagacg aaggaacagc agccgcattc gatgcagtcg aagatgtggt attgctgggt
     5581 cttgccgaaa tccttggcgc gcgagtgcca gtacagttcg aacggctgca gttcgtgcgg
     5641 gcaggcgcgg gcgcaggcgc cgcagcggat gcacggcatt tccggcgcct tcggcgggaa
     5701 caggcgcgcc gaatgggcga tcaggcagtt gctggtcttg acgatcggtg ccgtttcgtc
     5761 gggcaccaga aagcccatca tcggactgcc catgatgatg ccgtcggtgt acgggttggg
     5821 gctggccagg cgcagcagtt cgtccatcgg cgtgccgatc agcgcttcga cgttgtgcgc
     5881 ctggcggacg ttgccggtca gcgtgacgac gcgcgagatc agcggttcgc cgtggccgat
     5941 ggcacgccag gcggcgtagg cggtaccgac gttgaatacc tggacgccca tgtcggcggt
     6001 cgagcgcatg gcgccgggga cttccttgcc ggtcagcacg cggat
//
LOCUS       DAOSDX010000042         6005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4733_length_6005_cov_4.169244, whole genome shotgun sequence.
ACCESSION   DAOSDX010000042 DAOSDX010000000
VERSION     DAOSDX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4733_length_6005_cov_4.169244"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLN02_01590"
     CDS             <1..730
                     /locus_tag="PLN02_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOI51041.1"
                     /translation="HLNPGAAGPGSPRHSWRERLLALVETWPVLLVFLVVIGGIYGGL
                     FTPTEAAAIGALATGVVAWKSGGLKGKGLLECCFGTAKGTGMMFLILLGADTLNSFMA
                     ISQLPAEAANWVQQMGFGPWTVLLAIIVVYLLLGCVMDSLSMILLTIPIFLPIILGMD
                     YWGLSVDDKAIWFAIVALVVMEVGLITPPVGMNVYIIGGIAKDIPMATIFRGVMPFLA
                     WDVLRIGILLLFPSISLVALRVFG"
     gene            complement(776..1648)
                     /locus_tag="PLN02_01595"
     CDS             complement(776..1648)
                     /locus_tag="PLN02_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI51042.1"
                     /translation="MSILVPEFSLYGERQRETVREFLHVEEISARSAARNWQIDTHVH
                     NGLLQLLFVFSGSARIRLDSEEQDYGAAFAVVIPEGVIHAFKFQPGTEGCVLTLDAAG
                     LASASPDGSQMFSVLRGSAEVLALTRDDTAARVHGILEQIKAEFAHPGAGSTTLYVWL
                     VRSLLLLLVRATLRQADAQPDEGMRETAEGFRQMIEQHYLDHWPVSSYARQLNLSESR
                     LNRLCEKRFGRTAFALIQERLLLEAQRRLIYTGVPVSQLAYELGFSDPPYFCRFFKRL
                     TGHSPGAFRGHNRQ"
     gene            1990..3159
                     /gene="pobA"
                     /locus_tag="PLN02_01600"
     CDS             1990..3159
                     /gene="pobA"
                     /locus_tag="PLN02_01600"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HOI51043.1"
                     /translation="MKTSVCIIGGGPSGLMLSQLLHLQGIETVVLERQSREYVLSRIR
                     AGVLEHGFAELMREAQCGERMDREGEIHEGFIIAHNGKQSRVDLHKYSGGSSVVVYGQ
                     TELTRDLYEARDRMNGIVIHNAEDVQPHDLTGKPYVTYRQGDEIVRIDCDYVIGADGF
                     HGVSRKSIPKNVLKEYEKVYPFGWLGVLSRTKPVSPELIYARHERGFALCSLRSQVLS
                     RYYVQVPLSDSVEDWSDERFWEELKRRLPEDVAGRLITGPSIEKSIAPLRSFVAEPMR
                     YGNLFLAGDAAHIVPPTGARGLNSAASDIYYLYHALVDHYKKGDDNGLDNYSAKALAR
                     VWKAQRFSWWMTTMLHTFPDSLPYDQKLQQTDLDYLFSSEAAQRSLAENYVGLPF"
     gene            complement(3181..4077)
                     /locus_tag="PLN02_01605"
     CDS             complement(3181..4077)
                     /locus_tag="PLN02_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51044.1"
                     /translation="MDLKQLEYFVRVAELGSFTRASILLDIAQPALSRQVRMLELEFE
                     QNLLLRNGRGVVTTDAGKLLLEYGRGILYQVERLREELGRARGALVGHVALGLPPSLL
                     KILAVPIFREFKENFPAAKLAIREGMSTGMHESLVSGRLDVALLYNATPSPDVDLTPL
                     LEEELFLVSPETDSRPTTPVAIDELPDYPLIVPSRPNAIRMLIESALANLGLRPSIAF
                     EVDGINAILDLVGEGFGYAILSQTAVANLAGERKFRLRSVQGDGLKAKLSLGMNSHRQ
                     ITATQRALIELIQRVVATRLAQ"
     gene            complement(4106..4609)
                     /locus_tag="PLN02_01610"
     CDS             complement(4106..4609)
                     /locus_tag="PLN02_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOI51045.1"
                     /translation="MNSLIDGPSSQDKLRPSGFCYLIGRLDHAVNRRIRDALTALGLS
                     VAQYTALSFLESQSQISNAQLAERSLISPQSANEMIKLMEKKGWVERAPDPSHGRIVR
                     LSLTGAGQALLDQAHEQVARLEGAMLTKLSASQRGALQQNLRQVLHTLSAIMIDPTLA
                     GETGKDN"
     gene            4829..5665
                     /locus_tag="PLN02_01615"
     CDS             4829..5665
                     /locus_tag="PLN02_01615"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HOI51046.1"
                     /translation="MSKYEGRWSCVKVELEEGIAWVTLNRPEKRNAMSPTLNREMIDV
                     LQTLELDEEAVVLVLTGAGDSWTAGMDLKEYFRETDGKPEIFQERSRREACEWQWKLL
                     RMYNKPTIAMVNGWCFGGGFSPLVACDLAIAADEATFGLSEINWGIPPGNLVSKAMAD
                     TVGHRESLYYIMTGEPFSGQKAAEMGLVNKSVPRAQLRDEVVALAKKLLAKNPVVLRY
                     AKNGFKRCRELDWDQNEDYLYAKLDQCIGRDPEQGRKEGMKQFLDDKTLKPGLQTYQR
                     AK"
     gene            5731..>6005
                     /locus_tag="PLN02_01620"
     CDS             5731..>6005
                     /locus_tag="PLN02_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOI51047.1"
                     /translation="MRKVELLIGGESRPAANGASFERRNPVSGEVATLAAAATLADAD
                     AAVAAAAAAFPAWAALAPSARRALLLKAAEIMDRRAPEFVATGVDEAA"
BASE COUNT         1041 a         1846 c         2017 g         1101 t
ORIGIN      
        1 ccacctgaat ccgggtgccg ccggcccggg gtctccgcgt cactcctggc gcgagcgcct
       61 gctggcgctg gtcgagacct ggccggtatt gctggtcttc ctggtggtga tcggtggcat
      121 ctacggcggt ttgttcacgc cgaccgaagc ggctgccatc ggcgccctgg caaccggggt
      181 cgtcgcctgg aagtccggcg gcctgaaagg caaggggctg ctcgagtgct gtttcggcac
      241 ggccaaggga accggcatga tgttcctgat cctgctcggc gccgataccc tcaactcctt
      301 catggcgatt tcccagcttc ccgccgaggc ggccaactgg gtgcagcaga tgggtttcgg
      361 gccatggacg gtgttgctgg cgatcatcgt cgtctacctg ctgctcggct gcgtcatgga
      421 cagcctgtcg atgatcctgc tgaccatccc gatcttcctg ccgatcatcc tgggcatgga
      481 ttactggggg ctgagcgtcg acgacaaggc gatctggttc gccatcgtcg ctctggtcgt
      541 catggaggtc gggctgatca cgccaccggt cgggatgaac gtctatatca tcggcggcat
      601 cgccaaggac atcccgatgg cgactatctt ccgcggcgtc atgcccttcc tcgcctggga
      661 tgtcctgcgc atcggcatcc tgctgctctt cccgagcatt tccctggtgg cgctgcgggt
      721 cttcggctga gcggccatcc cgcattaccg gacggccgcc ccgggcggcc gtttttcatt
      781 gccggttgtg accgcggaag gcgcctggag agtgtccggt caggcgcttg aagaagcggc
      841 agaaatacgg cggatcggaa aatcccagtt cgtaggccag ctgggaaacc gggacgccgg
      901 tgtagatcag ccggcgctgc gcttccagca acaggcgctc ctgaatcagg gcgaacgccg
      961 tgcgaccgaa gcgtttctcg cacaggcggt tgagccggct ttcgctgagg ttgagctggc
     1021 gggcgtagct gctgaccggc cagtggtcga gatagtgctg ctcaatcatc tggcgaaagc
     1081 cttcggccgt ttcgcgcatg ccttcgtccg gttgcgcgtc tgcctggcgc agcgtggcgc
     1141 gcaccaggag cagcagcagc gagcgcacca gccagacgta gagcgtcgtg ctgccggcgc
     1201 ccgggtgcgc gaactcggcc ttgatctgct cgaggatgcc gtggacgcgg gccgccgtat
     1261 cgtcgcgcgt cagggcgagg acttcggcac ttccccgcag caccgaaaac atctggctgc
     1321 cgtccggcga ggcgctggcc agtccggcgg cgtcgagggt cagcacgcag ccctcggtgc
     1381 cgggctggaa tttgaaggca tggatgacgc cctcgggaat gaccacggca aacgcggcgc
     1441 cgtagtcctg ttcctcgctg tcaaggcgga tgcgtgcgct gccggaaaaa acgaagagca
     1501 gttgcagcag gccgttgtgt acatgggtgt ctatctgcca gttgcgcgcc gcgctacgcg
     1561 ccgagatttc ctcgacatgc agaaactcgc ggacggtttc gcgctggcgt tccccgtaaa
     1621 gcgaaaactc gggaaccaga atgctcatgg tcgttgctgt cctctcgttt ctgacaggtt
     1681 gttgctggaa tagtgcaatc ggttccggtg ctcgggcggg tggtggccgg gcctggccga
     1741 aaaccgtggc tgtgccttgg tttgatcttc ctggcgccgc atcaggaacg caatcaactt
     1801 tttctgtata gcagatatac ggatttcaac aaaagaaaag tccaattgat tgcccggatc
     1861 gtccaaggct ttgacgccgc cagcccctag tcttgcaagt agtccggagc cacggacacg
     1921 acgattcgag cgctgcgaaa tccctggctg gatgtccggg cgctcagtcc aatccaacag
     1981 gagacaagaa tgaaaaccag cgtttgtatc atcggcggtg gcccctcggg cctgatgctt
     2041 tcccagttgc tgcacctgca gggcatcgag acggtcgttc tcgaacggca gagccgcgag
     2101 tacgtgttga gccgcatccg tgccggcgtt ctcgagcacg gctttgccga gctgatgcgc
     2161 gaggcgcagt gcggcgagcg catggatcgc gagggggaaa tccacgaggg cttcatcatc
     2221 gctcacaacg gcaagcagag ccgcgtcgat ctgcacaagt acagcggcgg cagctcggtc
     2281 gtcgtctacg gccagaccga actgacccgc gatctctacg aagcgcgcga ccggatgaac
     2341 ggcatcgtca tccacaacgc cgaggacgtg cagccgcacg acctcaccgg caagccctac
     2401 gtgacttacc ggcagggcga cgagatcgtg cgcatcgact gcgattacgt gatcggcgcc
     2461 gacggcttcc acggcgtcag ccgcaagtcg attccgaaga acgtgctcaa ggagtacgag
     2521 aaggtctatc ccttcggctg gctgggcgtg ctgtcgcgca ccaagccggt gtcgccggaa
     2581 ctgatctatg ctcgccacga acgcggtttc gcgctgtgct cgttgcgctc gcaggtgctg
     2641 agccgttact acgtccaggt gccgctcagc gactcggtcg aggactggtc cgacgagcgc
     2701 ttctgggaag aactgaagcg ccgcctgccg gaagacgtgg ccgggcgcct gatcaccggc
     2761 ccgtcgatcg agaagtcgat cgccccgctg cgttccttcg tcgccgagcc gatgcgctac
     2821 ggcaatctgt tcctcgccgg cgacgccgcc cacatcgtgc cgccgaccgg tgcccgcggt
     2881 ctgaacagcg ccgcttccga catctactac ctgtatcacg cgctggtcga ccactacaag
     2941 aagggcgacg acaacggtct cgacaactat tcggccaagg cgctggcccg ggtgtggaag
     3001 gcgcagcgct tctcgtggtg gatgacgacg atgctgcaca ccttcccgga cagcctgcct
     3061 tacgaccaga aactgcagca gaccgatctc gactacctgt tctcgtcgga ggcggcgcag
     3121 cgctcgctgg ccgagaacta cgtcggcctg ccgttctgag ccggccttgc cacccgccgg
     3181 ctactgcgcc aggcgggtgg cgacgacacg ctggatcagc tcgatcagcg cccgctgcgt
     3241 cgcggtgatc tggcggtgcg agttcatccc cagcgacagc ttggctttca gcccgtcgcc
     3301 ctggaccgag cgcagccgga acttgcgctc gccggccagg ttggcgacgg cggtctgcga
     3361 caggatcgcg tagccgaaac cttcgccgac caggtcgaga atggcgttga tcccgtcgac
     3421 ctcgaaggcg atgctcggcc gcagcccgag gttggcgagc gcgctttcga tcagcatgcg
     3481 gatcgcgttg gggcggctgg gaacgatcag cggatagtcg ggcagctcgt cgatggcgac
     3541 cggggtggtc ggccggctgt cggtttcggg gctgaccagg aagagctctt cctcgagcag
     3601 cggcgtcagg tcgacgtcgg gcgatggcgt cgcgttgtag agcagggcga cgtcgaggcg
     3661 gccggaaacc agcgattcgt gcatgccggt gctcatgccc tcgcggatcg ccagtttggc
     3721 ggcggggaag ttctccttga attcgcggaa gatcggcacc gccaggatct tcagcaggct
     3781 cggtggcaaa ccgagggcga catggccgac cagcgcgccg cgcgcccggc cgagttcctc
     3841 gcgcaggcgt tcgacctgat agaggatgcc gcgaccatat tcgagcagca gcttgccggc
     3901 gtcggtggtg acgacgccgc ggccgttgcg caggagcagg ttctgttcga actccagttc
     3961 gagcatccgg acctggcggc tcagcgccgg ctgggcgatg tcgaggagga tggaggcgcg
     4021 cgtgaaactg ccgagctcgg cgacgcggac aaaatactcc agttgtttga ggtccattca
     4081 ggtgactccg gtgatggcgg gcagctcagt tgtccttgcc ggtttcgccg gcgagcgtgg
     4141 ggtcgatcat gatcgcgctg agcgtgtgca gtacctgccg cagattctgc tgcagcgcgc
     4201 cgcgttggct ggcggagagc ttggtcagca tggcgccttc caggcgggcg acctgctcat
     4261 gcgcctggtc gagcagcgcc tggccggccc cggtcaggct gaggcggacg atgcggccgt
     4321 gcgaggggtc gggcgcgcgt tcgacccagc ccttcttttc catcagcttg atcatctcgt
     4381 tcgccgactg tggcgagatc agcgagcgct cggcgagctg ggcgttgctg atctgcgatt
     4441 gcgattcgag gaaggagagc gcggtgtatt gggcgacgct caggccgagc gcggtcagcg
     4501 cgtcgcggat gcgccggttg accgcgtggt cgaggcggcc gatcaggtag cagaagccgc
     4561 tgggacggag cttgtcctgg ctgctcgggc cgtcgatcag tgagttcatg gcgattctcg
     4621 gtgaatttac ggtgcgggtc cgggaagtga gggttggcag gttgcctgat atcaaggccc
     4681 gatcttaccg cagccggctg ccgtctgggg agtgcagaaa attaatcatt ggggaatcga
     4741 ttgcagggta ttgcgttttt atttttatcc cttattattc gtctcatatc aggtagcctg
     4801 atatttccaa ccactaagga gacagaatat gtccaagtat gaaggccgct ggagctgcgt
     4861 gaaggtcgag ctcgaagaag gcattgcctg ggtcaccctg aaccgcccgg aaaagcgcaa
     4921 cgcgatgagc ccgacgctca atcgcgaaat gatcgacgtg ctgcagacgc tggaactcga
     4981 cgaggaggcc gtggtcctgg tgctgaccgg cgccggcgat tcgtggaccg ccggcatgga
     5041 tttgaaggaa tacttccgcg agaccgacgg caagccggaa atcttccagg aacgttcgcg
     5101 tcgcgaagcc tgcgaatggc agtggaagct cttgcgcatg tacaacaagc cgaccatcgc
     5161 catggtcaat ggctggtgtt tcggcggcgg cttctcgccg ctggtcgcct gtgacctggc
     5221 gattgccgcc gacgaagcga ccttcggcct gtccgagatc aactggggca tcccgccggg
     5281 caacctggtc agcaaggcga tggccgatac cgtcggtcac cgcgaatcgc tctactacat
     5341 catgaccggc gagccgttca gcggccagaa ggcggccgag atgggcctgg tcaacaagag
     5401 cgtgccgcgc gcccagttgc gtgacgaagt cgtcgccctg gccaagaagc tgctcgccaa
     5461 gaacccggtc gtgctgcgct acgccaagaa cggcttcaag cgctgccgcg aactcgactg
     5521 ggatcagaac gaggactacc tgtacgccaa gctcgaccag tgcatcggcc gtgatccgga
     5581 gcaggggcgc aaggaaggca tgaagcagtt cctcgacgac aagaccctga agccgggcct
     5641 gcaaacctat cagcgcgcca agtaagcgcc cacgtgcaaa aaataccggt ggccccacgg
     5701 ggcgccggaa tcactaccca ggaggagacg atgagaaaag ttgaattgct gattggcggc
     5761 gaaagccgcc ccgcggccaa cggcgccagc ttcgagcggc gcaatccggt cagcggcgaa
     5821 gttgcgacgc tggctgccgc cgctaccctg gccgacgccg atgcggcggt ggctgctgcc
     5881 gctgccgcct tcccggcctg ggcggcgctg gcgccgagcg cccgccgcgc gttgttgctg
     5941 aaagcggccg agatcatgga ccggcgcgcg ccggagttcg tcgccaccgg cgttgacgaa
     6001 gccgc
//
LOCUS       DAOSDX010000043         5998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4740_length_5998_cov_3.489988, whole genome shotgun sequence.
ACCESSION   DAOSDX010000043 DAOSDX010000000
VERSION     DAOSDX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4740_length_5998_cov_3.489988"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLN02_01625"
     CDS             complement(<1..277)
                     /locus_tag="PLN02_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI51048.1"
                     /translation="MNKQRKLNSPEGTEVFCTTREAAEMLHVSLRTVQLWVEAGVLKA
                     WKTDGGHRRLPLSSVQALIKERLGDTATPAPAADPAANDSGEFSVLVL"
     gene            complement(346..1065)
                     /locus_tag="PLN02_01630"
     CDS             complement(346..1065)
                     /locus_tag="PLN02_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51049.1"
                     /translation="MKHLRWLLCLACLLAAPAFAQNPAPAGETPTETRRDENNPDFYL
                     ELIASMQEKRLYYASLAHLDLFEQRWPKDRRALLLRADALRQTGDYAQARDCYRRLLE
                     QETSAGAHHGLGLIAAAEGDLALASVSLHEAARLAPIDARILNDLGYLQLLRGERAAA
                     RLNLFKASELAPDNRQVAANLALYLLLEGQGERAEQLMDKHGFAEAQRASVRSDAVRM
                     GRAGGEPGKPLTDGDRVEMVK"
     gene            complement(1062..2015)
                     /locus_tag="PLN02_01635"
     CDS             complement(1062..2015)
                     /locus_tag="PLN02_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI51050.1"
                     /translation="MNAALLPWLVAASVALAVLGVGGLVYGLFLAAQRKILRQRRFAR
                     LDQMLAPAGEGSEAIREAEELSFAEKLLLLFSGRLPGAAGGEADSEDRRLLIRAGYRG
                     LHALVFFQSLRWLTLLAVLLGFGGYALLAGSLQDGLRGVALAILIFLGSRYLLRYLAH
                     RRMTMLAEELPVFVDFLRMMHSVGISFEQAITLFAGDSRLGLPVLSNELGAVSLSIRS
                     GRSRSDALQLMARQLDVDDLSELVALICHTDYYGAAVQEPLRQFSQRLTERKRFQMQE
                     YVGKLATRMVIVMVLFLLPALIIVTAGPGFVSVIKALGDMA"
     gene            complement(2018..2932)
                     /locus_tag="PLN02_01640"
     CDS             complement(2018..2932)
                     /locus_tag="PLN02_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI51051.1"
                     /translation="MPELVWLVLAASFCLLAAAFLFLGGALRRAPGSGRRMSEVLARH
                     EAPFDGKAPAAASGWIPSWFERYLQRAGLPETPLVYGALLAPGAVLFLFAELLFGFAY
                     ALAGILIVYPFLLWSFVQWRIAVYRQQVVDQLPVFLESVSRILSVGCSLELAFRNASE
                     ECDDPLLTITRQVLLRTRAGQSLEDAMMQVADVYAIRELGFVASVFHLGIRYGGNAHA
                     VLERLSTTMRERRRSQQELKAMTAETRASAWILSALPVVVAILTLASNPGYLLGMWID
                     PFGRKILLGALAMQIAGMALLFRMARID"
     gene            complement(2925..4226)
                     /locus_tag="PLN02_01645"
     CDS             complement(2925..4226)
                     /locus_tag="PLN02_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOI51052.1"
                     /translation="MDFDFALGGDPRGDFRQSDEFQDIKAVVHQRLVERIEELGVDFA
                     TWAPHVTQRFVAQEVEDFVAANRVPLNVTDIQVIVKELNDELTGLGPLQELMNDDAVA
                     DILVNGYKEVFIEKNGLLQKSRVRFIDNRHLMRIVQRMIAPLGRRVDEGSPMVDARLP
                     NGGRLNVIIPPVSLNGPVVSIRKFRSKPLQASDMLAYGTLNQEMLDFLAHAAEMRCNI
                     LISGGTGSGKTSFLNMLAQYIREGERVITIEDAAELQLLHEHVVRLESRPGGLENVAE
                     VTPRDLLRNSLRMRPDRIIVGEVRGGEAVEMLQAMNTGHDGSMSTIHTNSPRECLHRL
                     EMLLHFGGWQGSEFNLRRQIVGAIDLIVQLARLPSGARRVMSISQITGMQDEVVAMQE
                     LFRFEPAANGRDGTFVSGCKPQSLKLKRYAAFPGRPGGNGA"
     gene            complement(4231..5418)
                     /locus_tag="PLN02_01650"
     CDS             complement(4231..5418)
                     /locus_tag="PLN02_01650"
                     /inference="COORDINATES: protein motif:HMM:NF028278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51053.1"
                     /translation="MEQRFIVVTEHEAHFNALSEAIQSQGVSVWLHWNSDVLVQLVGS
                     MGTNVVFIEFAESRLAQARDLAGKLLSVYPRLWIIGFAQRADNSLIIEAMRAGARDFV
                     ELQSAPEIICRSLRQVLAQSPQLPAQSSGTMISLLGARPGVGTSTAAVHLALLLKKEV
                     MPGEPVLLLDFGFPAADAALYLDTKVNFNFCDAVRSLRRFDHALLNSAFAHHKSGLSL
                     LSLPQNLAELRDVSPVDAMTLLSLLKSYFKVVVVDLGGFAGTDLLLYALGVSDRTLLI
                     SEQSIPSVFSARQMLASFRERGVETERIGLLTGKYDTRIEMSSAQLAQHLQLHWVADL
                     PQRNERLIACANQGKLLYELAPNDPYVNVLRDLAARESGGTAAAVPGGGGWRRRLQGL
                     IGG"
     gene            complement(5436..>5998)
                     /locus_tag="PLN02_01655"
     CDS             complement(5436..>5998)
                     /locus_tag="PLN02_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HOI51054.1"
                     /translation="SGRDAEFLAGGEFPVPVPQALGQTTIQYKPYGIRLQVTPTELAD
                     NRIALTVAPEVSDLNFENGITINGVSVPSLSTRRATTSIELGNGESFVIGGLVSQAMM
                     RNVNKIPGLGDLPLLGAFFKSISLSREDKELLFIVTPRLVRPLAAGTDLGPMPGEELA
                     NRESGLWRDWLLSDQDVNTRYTGFSK"
BASE COUNT          999 a         1938 c         2089 g          972 t
ORIGIN      
        1 cgagcaccag caccgagaac tcgccgctgt cgttggccgc cggatcggct gccggcgccg
       61 gcgtcgcggt gtcgcccagg cgttccttga tcagcgcctg taccgaactg agcggcagac
      121 ggcgatggcc gccgtcggtc ttccacgcct tgaggacgcc ggcctcaacc cagagctgca
      181 cggtgcgcag cgaaacgtgc agcatttcgg ccgcctcgcg ggtggtgcag aagacttccg
      241 tgccttcggg actattgagt tttcgctgtt tattcatttt gtctcgatta ccgtggttga
      301 tcggttggaa ttggggctaa aacgccaagg ctttcaatat tagcgtcatt tcaccatttc
      361 cacgcgatcg ccgtcggtaa gcggcttgcc gggttcgccg ccggcccggc ccatgcgaac
      421 agcatcgctg cgcaccgagg cgcgctgggc ctcggcgaaa ccgtgcttgt ccatcagttg
      481 ttcggcgcgc tcgccctgcc cttcgagcag caggtagagc gccagattgg cggcgacctg
      541 gcggttgtcc ggcgccagtt cggaagcctt gaacaggttc aggcgggcgg cggcgcgctc
      601 gccgcgcagc agttgcaggt aaccgaggtc gttgaggatg cgtgcgtcga tcggcgccag
      661 ccgggccgcc tcgtgcagcg atacggaagc cagcgccaga tcgccctcgg cggcggcgat
      721 caggccgagg ccgtgatggg cgccggccga cgtctcctgc tccagcaggc gccggtagca
      781 gtcgcgcgcc tgggcgtagt cgccggtctg gcgcagcgcg tcggcgcgca gcaggagcgc
      841 ccggcgatcc ttcggccagc gctgctcgaa caggtcgagg tgggcgagcg acgcgtaata
      901 gagccgcttt tcctgcatgc tggcgatcag ctcgaggtag aagtccgggt tgttctcgtc
      961 gcgccgcgtc tccgtcggcg tttcgccggc cggcgccggg ttctgggcga aagccggggc
     1021 cgccagcaga caggcgagac agagcagcca gcgcaagtgt ttcatgccat gtctcccagg
     1081 gccttgatca ccgaaacgaa acccggcccg gcggtgacga tgatcagcgc cggcagcagg
     1141 aaaagaacca tgacgatgac catgcgcgtc gccagcttgc cgacgtattc ctgcatctgg
     1201 aagcgcttgc gctcggtcag gcgctgcgag aactggcgca gcggctcctg caccgcggcg
     1261 ccgtagtaat cggtgtggca gatcagggcg accagttcgc tgaggtcatc gacgtcgagc
     1321 tggcgggcca tcagttgcag cgcgtcgctg cgcgagcggc cgctgcggat cgacaggctg
     1381 accgcgccca gttcgttcga cagcaccggc aggccgagcc gggaatcgcc ggcaaacagc
     1441 gtgatcgcct gttcgaagct gatgccgacg ctgtgcatca tgcgcaggaa atcgacgaac
     1501 accggcagtt cctcggccag catcgtcatc cgccggtgcg ccaggtagcg cagcaggtag
     1561 cgcgaaccga ggaagatcag gatcgccagc gccacgccgc gcagcccgtc ctgcaggctg
     1621 ccggcgagca gcgcgtagcc gccgaaaccg agcagcacgg caagcagcgt cagccagcgc
     1681 aggctctgaa agaagaccag cgcgtgcagg ccgcggtagc cggcccggat cagcaaccgg
     1741 cggtcttcgg aatcggcctc gccaccggca gcgccgggca gacgcccgga gaagaggagg
     1801 agcagcttct cggcgaagct cagttcctcg gcctcgcgga tcgcctcgct gccttcgcca
     1861 gccggcgcca gcatctggtc gagacgggcg aagcggcgct ggcgcaggat cttgcgctgg
     1921 gcggcgagga acaggccata gaccagcccg ccaacaccga ggacggcaag ggcgacgctg
     1981 gcggcgacca accagggcag gagggcggcg ttcatgttca gtctatcctc gccatgcgga
     2041 acagcagcgc catcccggca atctgcatgg ccagcgcgcc gagcaggatc ttgcggccga
     2101 acgggtcgat ccacatgccg agcaggtagc ccgggttgga cgccagcgtc aggatcgcca
     2161 cgaccaccgg cagggcactc aggatccacg cgctggcgcg ggtttcggcg gtcatcgcct
     2221 tgagttcctg ctggctgcgg cggcgctcgc gcatcgtcgt cgacagccgt tcgagcacgg
     2281 cgtgcgcgtt gccgccgtag cggatgccga gatgaaacac ggaagcgacg aagccgagtt
     2341 cgcggatcgc gtagacgtcg gccacctgca tcatcgcgtc ttccagcgac tggccggcgc
     2401 gggtgcgtag caacacctgg cgggtgatgg tcagcagcgg gtcgtcgcac tcctcgctgg
     2461 cgttgcggaa cgccagttca aggctgcagc cgacgctgag gatgcgcgat acgctttcca
     2521 ggaaaaccgg caactggtcg accacctgct gccgatagac agcgatccgc cactggacga
     2581 agctccacaa caggaaggga tagacgatca ggatgccggc cagcgcgtag gcaaaaccga
     2641 acagcagttc ggcgaacagg aagaggacgg cgcccggcgc cagcaaggcg ccgtagacca
     2701 gcggcgtttc cggcaggccg gcgcgctgca ggtagcgctc gaaccacgac ggaatccagc
     2761 cgctcgccgc cgccggtgcc ttgccgtcga acggcgcctc gtggcgggcc agcacttcgc
     2821 tcatccgccg cccgctgccc ggcgcccggc gcagggcgcc gccgaggaag aggaaggcgg
     2881 cagccagcag gcagaaactg gcggcgagga cgagccagac cagttcaggc accgttgcct
     2941 cccggccggc cggggaaggc ggcgtagcgt ttcagcttca gcgactgcgg cttgcagccg
     3001 ctgacgaaag tgccatcgcg gccgttcgcc gcgggctcga agcggaacag ctcctgcatg
     3061 gcgacgactt cgtcctgcat gccggtgatt tggctgatcg acatcacccg gcgggcgccg
     3121 ctcggcaggc gggcgagctg gacgatcagg tcgatggcgc cgacgatctg ccgccgcagg
     3181 ttgaactcgc tgccctgcca gccgccgaag tggagcagca tttccaggcg gtgcaggcat
     3241 tcgcgcggcg aattggtgtg aatcgtcgac atcgagccgt cgtggccggt attcatcgcc
     3301 tgcagcatct cgaccgcttc gccgccgcgc acctcgccga cgatgatgcg gtccgggcgc
     3361 atgcgcaggc tgttgcgcag gaggtcgcgc ggcgtcactt cggcgacgtt ctccagcccg
     3421 ccggggcgcg attcgaggcg gacgacgtgt tcgtgcagca actgcagttc ggcggcgtcc
     3481 tcgatggtga tcacccgctc gccctcgcgg atgtactggg ccagcatgtt gaggaaggag
     3541 gtcttgcctg agccggtacc gccgctgatc aggatgttgc agcgcatttc ggcggcgtgc
     3601 gccaggaaat cgagcatttc ctggttgagc gtgccgtagg cgagcatgtc ggaagcctgc
     3661 aacggcttgc tgcggaactt gcggatcgac accaccggcc cgttcagcga caccggcgga
     3721 atgatcacgt tgagccggcc gccgttgggc aggcgggcgt cgaccatcgg gctgccttcg
     3781 tcgacgcggc gaccgagcgg cgcgatcatg cgctggacga tgcgcatcag gtggcggttg
     3841 tcgatgaagc ggacgcggct tttctggagc aggccgttct tctcgataaa gacttccttg
     3901 tagccgttga ccaggatgtc ggcgacggcg tcgtcgttca tcagttcctg cagcgggccg
     3961 aggccggtca gctcgtcgtt cagttccttg acgatgacct gtatgtcggt gacgttgagc
     4021 ggtacccggt tggcggcgac gaaatcctcg acctcctggg cgacgaagcg ctgggtgaca
     4081 tgcggcgccc aggtcgcgaa atcgacgccg agttcctcga tgcgttcgac caggcgctgg
     4141 tgcaccaccg ccttgatgtc ctggaattcg tcggactggc gaaaatctcc gcgcggatcg
     4201 ccgccgaggg cgaaatcgaa atccatgccg tcagcctccg atcaatccct gcaggcgccg
     4261 ccgccagccg ccgccgcccg gtacggcggc cgccgtgccg ccgctctcgc gggcggcgag
     4321 gtcgcgcagc acgttgacgt aggggtcgtt gggcgccagc tcgtagagca gcttgccctg
     4381 gttggcgcag gcgatcagcc gctcgttgcg ctgcggcagg tcggcgaccc agtgcagttg
     4441 caggtgctgg gcgagctgtg ccgagctcat ttcgatgcgc gtatcgtact tgccggtgag
     4501 caggccgatg cgctcggtct cgacgccgcg ttcgcggaag ctggccagca tctggcgcgc
     4561 cgagaacacc gacgggatgc tctgctcgct gatcagcagg gtgcggtcgc tgacgccgag
     4621 cgcgtagagc agcaggtcgg tgccggcgaa accgccgaga tcgacgacca ccaccttgaa
     4681 gtaggacttg agcaggctga gcagggtcat cgcgtcgacc ggcgagacgt cgcgcagctc
     4741 ggccaggttc tgcggcagcg agagcagcga gaggccgctc ttgtggtgcg cgaaggccga
     4801 gttgagcagg gcgtggtcga agcggcgcag gctgcgcacg gcgtcgcaga aattgaaatt
     4861 gaccttggtg tcgaggtaaa gcgcggcgtc ggcggccggg aagccgaaat cgagcagcag
     4921 caccggctcg ccgggcatca cttccttctt cagcagcaac gccagatgca ccgccgccgt
     4981 cgaggtgccg acgccgggcc gggcgccgag caggctgatc atcgtcccgc tcgattgcgc
     5041 cggcagttgc ggcgactggg cgagcacctg gcgcagcgag cggcagatga tttccggtgc
     5101 cgactgcagc tcgacgaaat cgcgcgcgcc ggcgcgcatc gcctcgatga tcaggctgtt
     5161 gtcggcgcgc tgcgcgaagc cgatgatcca caggcgcgga tagaccgaca agagcttgcc
     5221 ggcgagatcg cgggcctgcg ccagacggct ttcggcgaat tcgatgaaga ccacgttggt
     5281 ccccatcgaa ccgaccagct ggaccaggac gtcgctgttc cagtgcagcc agaccgacac
     5341 cccctgactc tgtatggctt cgctgagcgc attgaaatga gcttcgtgct cggtcaccac
     5401 gatgaagcgt tgttccatgc ctgttctccg tcggcttact tggaaaatcc ggtgtagcgg
     5461 gtattcacat cctgatcgga cagcagccag tcgcgccaca gcccggattc gcggttcgcc
     5521 agttcctcgc ccggcatcgg cccgaggtcg gtgcccgccg ccagcggccg caccaggcgc
     5581 ggcgtgacga tgaacagcag ttccttgtcc tcgcgcgaca ggctgatgct cttgaagaag
     5641 gcgccgagca gcggcaggtc gccgaggccg ggaatcttgt tcacgttgcg catcatcgcc
     5701 tggctgacca ggccgccgat gacgaagctc tcgccgttgc ccagctcgat gctggtggtc
     5761 gcccgccgcg tgctcaggct gggaacgctg acgccgttga tggtgatgcc gttctcgaag
     5821 ttgaggtcgc tgacttccgg cgccacggtg agcgcgatac ggttgtcggc gagctcggtc
     5881 ggcgtcacct gcaggcggat gccgtagggt ttgtactgga tcgtcgtctg cccgagcgcc
     5941 tgcggcaccg gcaccgggaa ctcgccgcca gcgaggaatt cggcgtcgcg gccactct
//
LOCUS       DAOSDX010000044         5997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4742_length_5997_cov_3.381016, whole genome shotgun sequence.
ACCESSION   DAOSDX010000044 DAOSDX010000000
VERSION     DAOSDX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4742_length_5997_cov_3.381016"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PLN02_01660"
     CDS             <1..1527
                     /locus_tag="PLN02_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI51055.1"
                     /translation="LAQRARLPLLPTRNAQPSQAAVEERGLVVHSAAPIHDAQGRLLG
                     VLAGGVLLNGNLEFIDHLNEIVYPDDKLLDSVGTATLFLGDVRIATNVRLFEGERAIG
                     TRVSQAVRDSVLGRGETWLARAFVVQDWYYSGYRPLLDSRGERVGMLYVGFLETPFVA
                     AKKSAFVGIVALFALAMLIAAIFAVLWARRIFRPIERMHTTMNAIEAGHTEARVGPID
                     NADEIGELARHFDRLLERQQAQALALQRWGESLDGKVAQRTTELEEALANLRSTQGKL
                     ITNEKMAAIGQLTAGVAHEINNPIAVIQGNLELARELLGAQAKPVEPEFRLILDQVHR
                     IRLIVTKLLQFARPQEYVGYLEPVNASQLIHDCLLLVGHLLRKGNISIEQEISTNRLV
                     TCNKNELQQVMINLLANAIQVMPDGGRLRLRAEEWDEADMPIGLRLSVADSGPGIPEA
                     EREHLFEPFFTAHKPGGHGLGLWVSKSLVERYDGRITVDCPDSGGAVFTVWLRCEPLG
                     "
     regulatory      1586..1655
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1729..5388
                     /gene="metH"
                     /locus_tag="PLN02_01665"
     CDS             1729..5388
                     /gene="metH"
                     /locus_tag="PLN02_01665"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOI51056.1"
                     /translation="MQPDRSTELHALLEQRLLVLDGAMGTMIQRHGLQEKDYRGERFR
                     DHPHDLKGNNDLLVLTRPDIIGGIHREYLEAGADILETCTFNSTAVSQADYNLTELVY
                     ELNFEGAALARRLCDEFTAHNPAKPRFVAGVLGPTSRTASISPDVNDPGYRNVTFDEL
                     VANYVEATTGLIEGGADILLVETVFDTLNAKAALFAIEKYFDIAGRRWPVMISGTITD
                     ASGRTLSGQTAEAFWNSLRHVQPLSFGLNCALGASELRQYVEELSRVCDCYVSAHPNA
                     GLPNAFGGYDETPEQLADEIAGWAERGIVNIVGGCCGTSPDHIRAIAERVAAVAPRQR
                     PVIAPALRLSGLEAFNVTADSLYVNVGERTNVTGSRAFARMILEGRFDDALAVARQQV
                     ENGAQVIDINMDEAMLDSLAAMDKFLKLIASEPDISRVPIMIDSSKWEVIEAGLKCIQ
                     GKGIVNSISMKEGEARFLEQAKLARRYGAAVIVMAFDEKGQADTYARKIEICGKAYEL
                     LTGMGFPAEDIIFDPNIFAIATGIPEHDNYAVDFIESVRWIKENLPHAHISGGVSNVS
                     FSFRGNDPVREAIHTVFLYHAIKNGMTMGIVNAGMLGVYDDLEPELRAKVEDVVLNKN
                     PGAGEALVDFAQTVKEGKAKDAGPDLSWREQSVEKRLEHALIKGITDFVVADTEEVRV
                     KLEGEGKPPLAVIEGPLMSGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLLPYIEA
                     EKARTGLGSKGKILMATVKGDVHDIGKNIVGVVLGCNGYDVIDLGVMVPCDNILKAAL
                     EHKVDIIGLSGLITPSLEEMAHVAGEMKRLGMNQPLLIGGATTSRAHTAIKIATNTDS
                     PVVYVPDASRAVGVATKLLSTDQKAGYVAEIAAEYETVRAEHAGRKGATLVSLADARA
                     NRFTWNEQYTPVKPAQLGRQTLDIPLADLVPYIDWSPFFQSWDLAGRYPAILNNDVVG
                     ETARQLFHDAQAMLAKIVAEKWLTARAVFGLYPARGDNEDIVLYTDESRSVEATRWVG
                     LRQQIKQPAGRFNTALADFVGENDYAGAFAVTAGLGIDNRVAAFEAAHDDYSAIMLKA
                     LADRFAEAAAEWLHAQVRTHYWGYAKDEKLDNDALIAEAYSGIRPAPGYPACPDHTTK
                     RELFALLDAPAIGMGLTESCAMTPAAAVSGFYIGHPGAKYFAIPKIGRDQLEDWATRK
                     GMDVKEAERWLAPLL"
     gene            5401..5640
                     /locus_tag="PLN02_01670"
     CDS             5401..5640
                     /locus_tag="PLN02_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HOI51057.1"
                     /translation="MAKRFPLHPKHPERICWGCDKYCAARDLQCGNGSGRTMHPAEML
                     GDDWYTWGDWGIEPDENDPSAAARPLTAAEDKADK"
     gene            complement(5664..>5997)
                     /locus_tag="PLN02_01675"
     CDS             complement(5664..>5997)
                     /locus_tag="PLN02_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51058.1"
                     /translation="GGACLIDMPGMRELGMLDVSEGIADSFADIRALARDCRFADCSH
                     ESEPGCAIQRAIAEGEVDAEHFRNYQKLLRESAFHEMSLHEKRDKDRAFGKLVRAAGK
                     ARGRRHEY"
BASE COUNT         1124 a         2174 c         1789 g          910 t
ORIGIN      
        1 ctcgcccaac gtgcgcgcct accgctgctg ccgacccgca acgcgcaacc cagccaggcc
       61 gccgtcgaag agcgcggcct cgtcgtgcac agcgccgccc cgatccatga cgcccaaggt
      121 cgcctgctcg gcgtgctggc gggtggcgtg ctgctcaacg gcaacctcga attcatcgac
      181 cacctcaacg aaatcgtcta tcccgacgac aagctgctcg acagcgtcgg caccgcgacg
      241 ctgttcctcg gcgacgtgcg catcgccacc aacgtccgcc tgttcgaggg cgagcgggcg
      301 atcggcacgc gcgtctcgca ggcggtacgc gatagcgtgc tcggccgcgg cgaaacctgg
      361 ctagcccgcg ccttcgtcgt ccaggactgg tactactccg gctaccggcc gctgctcgac
      421 agccgcggcg agcgggtcgg catgctctac gtcggcttcc tcgaaacgcc cttcgtcgcc
      481 gccaagaaat ccgccttcgt cggcatcgtc gccctcttcg cgctggccat gctgatcgcc
      541 gcgatcttcg cggtgctctg ggcgcgccgc atcttccggc cgatcgagcg gatgcacacg
      601 acgatgaacg cgatcgaggc cggccacacc gaggcccgcg tcggcccgat cgacaacgca
      661 gacgaaatcg gcgaactggc ccgccacttc gaccgcctgc tggaacgcca gcaggcccag
      721 gcgctggccc tgcaacgctg gggcgagtcg ctcgacggca aggtggcgca acgcaccacc
      781 gaactggaag aagcactggc caacctgcgc tcgacccagg gcaagctgat caccaacgag
      841 aaaatggccg ccatcggcca attgaccgcc ggcgtcgccc acgaaatcaa caatccgatc
      901 gccgtcatcc agggcaacct cgaactcgcc cgcgaactgc tcggcgcgca agccaaaccg
      961 gtcgaacccg aattccggct gatcctcgac caggtgcacc gcatccgcct gatcgtcacc
     1021 aagctgctgc agttcgcccg tccgcaggaa tacgtcggct acctcgaacc ggtcaatgcc
     1081 agccagttga tccacgactg cctgctgctg gtcggccact tgctgcgcaa gggcaacatc
     1141 tcgatcgaac aggaaatcag caccaaccgg ctggtcacct gcaacaagaa cgagttgcag
     1201 caggtgatga tcaacctgct cgccaacgcg atccaggtca tgcccgacgg cggccgcctg
     1261 cgcctgcgcg ccgaggaatg ggacgaagcc gacatgccga tcggcctgcg cctgagcgtc
     1321 gccgactccg gcccgggcat ccccgaagcc gagcgcgaac acctgttcga gcccttcttc
     1381 accgcccaca agccgggcgg ccacggtctc ggcctgtggg tcagcaagtc gctggtcgaa
     1441 cgctacgacg gccgcatcac cgtcgactgc ccggacagcg gcggcgccgt gttcaccgtc
     1501 tggctgcgct gcgagccgct gggctgagcg actgtttttg cagggaaaac ttggctttcg
     1561 cgccgtcgac ggctacactt cgcctcctgc cgaggagcgc tgcgacctat ctgacgaggg
     1621 ccaggctcgg caacgatcaa cggcgctcgc aaaccctgtt ccactcgccg gtttgctgcg
     1681 ccgttcgctt ttggcggcgt caccggtgct ttgccgaggt aatccatgat ccagcccgac
     1741 cgctccaccg aactccacgc cctgctcgaa caacgcctgc tcgtcctcga cggcgcgatg
     1801 ggcacgatga tccagcgcca cggcctgcag gagaaggatt accgcggcga gcgcttcagg
     1861 gatcacccgc acgacctcaa gggcaacaac gacctgctgg tgctgacccg cccggacatc
     1921 atcggcggca tccaccgcga gtacctggaa gccggcgccg acatcctgga aacctgcacc
     1981 ttcaattcga ccgccgtctc gcaggccgac tacaacctga ccgaactggt ctacgagctg
     2041 aacttcgaag gcgccgcact ggcccgccgc ctgtgcgacg aattcaccgc ccacaacccg
     2101 gccaagccgc gcttcgtcgc cggcgtcctc ggcccgactt cgcgcaccgc gtcgatctcg
     2161 cccgacgtca acgaccccgg ctaccgcaac gtcactttcg acgaactggt cgccaactac
     2221 gtcgaagcga cgaccggcct gatcgagggc ggcgccgaca tcctgctcgt cgaaaccgtc
     2281 ttcgacacgc tcaatgccaa ggccgcgctg ttcgccatcg agaagtactt cgacatcgcc
     2341 ggccgccgct ggccggtgat gatctcgggc acgatcaccg acgcttccgg gcgcacgttg
     2401 tccgggcaga ccgccgaggc tttctggaat tcgctgcgcc acgtccagcc gctttccttc
     2461 ggcctcaact gcgcgctcgg cgccagcgaa ctgcgccagt acgtcgagga actctcgcgc
     2521 gtctgcgact gctacgtctc ggcccacccc aatgcgggcc tgccgaatgc cttcggcggc
     2581 tacgacgaaa ccccggaaca actggccgac gaaatcgccg gctgggccga gcgcggcatc
     2641 gtcaacattg tcggcggctg ctgcggcacc tcgcccgacc acatccgcgc catcgccgag
     2701 cgggtggctg ccgtcgcccc gcgccagaga ccggtgatcg caccggcgct gcgcctgtcc
     2761 gggctggaag ccttcaacgt caccgccgac tcgctctacg tcaacgtcgg cgagcgcacc
     2821 aacgtcaccg gctcgcgcgc cttcgcgcgg atgattttgg agggccgctt cgacgacgcg
     2881 ctcgccgtcg cccgccagca ggtcgaaaac ggcgcccagg tgatcgacat caacatggac
     2941 gaagcgatgc tcgattcgct cgccgcgatg gacaaattcc tcaagctgat cgcctcggaa
     3001 ccggacattt cgcgcgtgcc gatcatgatc gactcgtcga agtgggaagt gatcgaggcc
     3061 ggcctcaagt gcatccaggg caagggcatc gtcaattcga tctcgatgaa ggaaggcgaa
     3121 gccaggttcc tggaacaggc gaaattggca cgtcgctacg gcgccgccgt catcgtcatg
     3181 gccttcgacg aaaagggcca ggccgacacc tacgcgcgca agatcgaaat ctgcggcaag
     3241 gcctacgaac tgctcaccgg catgggtttc ccggccgaag acatcatttt cgacccgaac
     3301 atcttcgcca tcgccacggg catcccggaa cacgacaact acgccgtcga tttcatcgag
     3361 tcggtgcgct ggatcaagga aaacctgccg cacgcgcaca tttccggcgg cgtttccaac
     3421 gtctcgttca gcttccgcgg caacgacccg gtacgcgagg ccatccacac cgtcttcctc
     3481 taccacgcca ttaaaaacgg catgaccatg ggcatcgtca acgccggcat gctcggcgtg
     3541 tacgacgacc tggagccgga gctgcgcgcc aaggtcgaag acgtggtttt gaacaagaac
     3601 cccggcgccg gcgaggcgct ggtcgatttc gcccagacgg tcaaggaagg caaggccaag
     3661 gacgccggcc ccgatctctc ctggcgcgag cagtcggtcg aaaagcgcct ggaacacgcg
     3721 ctgatcaagg gcatcaccga tttcgtcgtc gccgacaccg aggaagtccg cgtcaagctg
     3781 gaaggcgaag gcaagccgcc gctggcggtc atcgaaggcc cgctgatgag cggcatgaac
     3841 gtcgtcggcg acctgttcgg cgccggcaag atgttcctgc cgcaggtggt caaatcggcg
     3901 cgcgtcatga agcaggcggt cgcccacctg ctgccctaca tcgaagccga aaaggcccgc
     3961 accggcctcg gctccaaggg taagatcctg atggcaaccg ttaaaggcga cgtgcacgac
     4021 atcggcaaga acatcgtcgg cgtggtgctg ggctgcaacg gctacgacgt catcgacctc
     4081 ggcgtcatgg tgccttgcga caacatcctc aaggcggcgc tcgaacacaa ggtggacatc
     4141 atcggcctct ccggcctgat caccccgtcg ctggaagaaa tggcccacgt cgccggcgag
     4201 atgaaacgcc tcggcatgaa ccagccgctg ctgatcggcg gcgcgacgac cagccgcgcc
     4261 cacaccgcga tcaagatcgc caccaacacc gacagcccgg tggtctacgt accggacgcc
     4321 tcgcgcgccg tcggcgtcgc taccaagctg ctgtcgaccg accagaaggc cggctacgtt
     4381 gccgaaatcg ccgccgagta cgaaaccgtg cgcgccgagc acgccggccg caagggcgcg
     4441 acgctggtca gcctggccga cgcccgcgcc aaccgtttca catggaacga gcagtacacg
     4501 ccggtcaaac cggcccaact cgggcgccag acgctggaca tcccgcttgc cgacctcgtc
     4561 ccctacatcg actggtcgcc cttcttccag agttgggacc tggccggccg ctacccggcc
     4621 atcctcaaca acgatgtggt cggcgaaacc gcccgccagt tgttccacga tgcgcaggcg
     4681 atgctggcga aaatcgtcgc tgaaaaatgg ctgactgccc gtgccgtctt cggcctctac
     4741 ccggcccgcg gcgacaacga ggacatcgtg ctgtacaccg atgaatcgcg tagcgtcgag
     4801 gccacccgct gggtcggcct gcgccagcag atcaagcaac cggccggccg tttcaacacc
     4861 gcgctcgccg atttcgtcgg tgaaaacgac tacgccggcg ccttcgccgt caccgccggc
     4921 ctcggcatcg acaatcgcgt cgccgccttc gaagccgccc acgacgacta ctcggcgatc
     4981 atgttgaagg ccctggccga ccgctttgcc gaagccgccg ccgagtggct gcacgcgcaa
     5041 gtacgcactc actactgggg ctacgccaag gacgaaaagc tcgacaacga tgcgctgatc
     5101 gccgaagcct acagcggcat ccgccccgcc cccggctacc cggcctgccc cgaccacacc
     5161 accaagcgcg aactgttcgc cctgctcgac gcgccggcga tcggcatggg cctgaccgaa
     5221 tcctgcgcga tgaccccagc cgccgccgtc tccggtttct acatcggcca cccgggcgcc
     5281 aagtacttcg ccatcccgaa gatcggccgc gatcaactgg aagactgggc gacgcgcaag
     5341 ggcatggacg tcaaggaagc cgaacgctgg ctggcgccgc tgctgtaatg agggggagcg
     5401 atggccaagc gcttcccgct ccaccccaag caccccgagc gcatctgctg gggctgcgac
     5461 aaatactgtg cggcccgcga cctgcaatgc ggcaacggct ccggccgcac catgcacccc
     5521 gccgaaatgc tcggcgacga ctggtacacc tggggcgact ggggcatcga gccggacgaa
     5581 aacgatccgt cagcggcggc aaggccgctg acggctgctg aggacaaggc ggacaagtag
     5641 cccgccttcg ctcccatcag acctcaatac tcgtgccgcc gccccctggc cttgccggcg
     5701 gcgcgcacca gcttgccgaa ggcccgatcc ttgtcgcgtt tctcgtgcag ggacatctcg
     5761 tgaaacgccg attcccgcag cagcttctgg tagttgcgga aatgctcggc gtccacttca
     5821 ccctcggcga tggcgcgctg gatagcgcaa cccggctcgc tctcgtgcga gcagtcggcg
     5881 aagcggcaat cgcgcgccag cgcccggatg tcggcgaaac tgtcggcgat gccctcgctg
     5941 acatcgagca tgcccagttc gcgcatgccc ggcatgtcga tcaggcaggc accgccc
//
LOCUS       DAOSDX010000045         5964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4774_length_5964_cov_3.240142, whole genome shotgun sequence.
ACCESSION   DAOSDX010000045 DAOSDX010000000
VERSION     DAOSDX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4774_length_5964_cov_3.240142"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLN02_01680"
     CDS             complement(<1..388)
                     /locus_tag="PLN02_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HOI51059.1"
                     /translation="MAEPPNQKAVANGLCAHCPLYPSFLEAGRCQPGEACIRAHSGRQ
                     IDRFLRRNPDEAAHYLDDIFWERRAIAARHAPVDRIFRLKSDSDEVVRRVVASRLPVD
                     QLAAMADDPDREVRLSVAARLPEEQLP"
     gene            complement(457..732)
                     /gene="fdxB"
                     /locus_tag="PLN02_01685"
     CDS             complement(457..732)
                     /gene="fdxB"
                     /locus_tag="PLN02_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HOI51060.1"
                     /translation="MADYTALTRAGNPWIPQFVTALDEEKCIGCGRCYKVCPRDVLNL
                     IDSDEDDDEDESAAAKMTLKDLGDCIGCSACGRVCPKNCFTYEALAA"
     gene            complement(1023..1283)
                     /locus_tag="PLN02_01690"
     CDS             complement(1023..1283)
                     /locus_tag="PLN02_01690"
                     /inference="COORDINATES: protein motif:HMM:NF040002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6129 family protein"
                     /protein_id="HOI51061.1"
                     /translation="MTPERLAEVVSAVERGDNASTLRASFSELIFTECSEDDISPRLQ
                     AVATTPAALIYLITGISGHCLEATSDLAVANGVIIASRDSDE"
     gene            complement(1285..2010)
                     /locus_tag="PLN02_01695"
     CDS             complement(1285..2010)
                     /locus_tag="PLN02_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOI51062.1"
                     /translation="MSQPVASREAAYRIALAARALEGLEVRALVNALAEKLELPITES
                     KLSRLTVEDLREILAGDHADENCTVGVSPEALKETVRILWGEDVAGSDKPTPEAYQEG
                     DMPGSIRVAIAANRGENLDGHFGSCENFLIYQLSPTEIRLIDVRSGLDADAAEDRNAA
                     RAAVINDCQIVYLQSIGGPAAAKVVRAGVHPLKKPKGGAAREVLGELQGALLNPPPWL
                     ARIMGIAPGSLARYAEALDAEEA"
     gene            complement(2077..2268)
                     /locus_tag="PLN02_01700"
     CDS             complement(2077..2268)
                     /locus_tag="PLN02_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI51063.1"
                     /translation="MAMYIDPDMCTACDDCRPICPTKAIKKGKVFYTIDAATCTECEG
                     ENDTPMCVNVCPSGCISPA"
     gene            complement(2285..2512)
                     /gene="nifT"
                     /locus_tag="PLN02_01705"
     CDS             complement(2285..2512)
                     /gene="nifT"
                     /locus_tag="PLN02_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HOI51064.1"
                     /translation="MANIMIRQNAAGGLVFYLPKRDLEAAIESIEFDTAEKWGGVLML
                     ENGGSYYVDPIAKPARLPISVRARRLGAAED"
     gene            complement(2569..4137)
                     /gene="nifK"
                     /locus_tag="PLN02_01710"
     CDS             complement(2569..4137)
                     /gene="nifK"
                     /locus_tag="PLN02_01710"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HOI51065.1"
                     /translation="MQTVEKIKPCYPLFRDDDYKATLTNKKAQFEEGHSKDKVWEIFN
                     WTTTQEYQELNFKREALTVNPAKACQPLGSALCGLGFKKSLVYIHGSQGCVAYFRTYF
                     NRHFKEPIAFIADSMTEDAAVFGGQKNVHEGLANATKLYGAEMIAMSTTCIAEVIGDD
                     LNAFINNAKNEGHLDKDFPVPFAHTPSFVGSHVTGWDNMEEGILRYFTLNTMEGKVVG
                     SNGKINIVPGFETYLGNYRVIKRYMKEMGVDYTMLSDPEEVLDTPADGEFRMYAGGTT
                     MDEVKDAPNAITTFLVQPLTCEKTKKFVENTWNHEVPKTNIPMGVEWTDEFLMKVSEA
                     TGKPIADSIKLERGRLIDMMTDSHAWLHGKKMALYGDPDFLMGMVKLLLEFGIEPTHI
                     VCNNGSKKWAKAMEKLLASSPYGVNGKAYPGADLWHLRSLCFTDKPDFLIGNSYGKYI
                     QRDTVHKGKEFEVPLIRIGFPIFDRHHLHRMTTLGYEGAMYVLTTLTNAVLERLDDQT
                     REMGVTDYNHDLVR"
     gene            complement(4203..5675)
                     /gene="nifD"
                     /locus_tag="PLN02_01715"
     CDS             complement(4203..5675)
                     /gene="nifD"
                     /locus_tag="PLN02_01715"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein alpha chain"
                     /protein_id="HOI51066.1"
                     /translation="MAALTREESAALIAEVLEVYPEKAKKDRAKHLMVNDTTLESSKK
                     CITSNRKSLPGVMTMRGCAYAGSKGVVWGPIKDIISISHGPIGCGQYARAGRRNYYVG
                     TTGADAFCEMNFTSDFQERDIVFGGDKKLAKLIEEIEILFPLNKGISVQSECPIGLIG
                     DDIEAVSKKAAAQIGKPVVPVRCEGFRGVSQSLGHHIANDSVRDWILSNRDGQAFETT
                     PYDVAILGDYNIGGDAWASRILLEEMGLRVVAQWSGDGTLAEMENTPKVKLNLLHCYR
                     SMNYISRHMEEKYGIPWMEYNFFGPTKIKESLRKIASFFDDKIKAGAEAVIEKYTPMM
                     DEVIAKYKPRLEGKKVMLYVGGLRPRHVVGAYEDLGMEVVGTGYEFAHNDDYDRTIKE
                     MGDATLIYDDVTGYEFEEFVKACKPDLIGSGIKEKYVFHKMGIPFRQLHSWDYSGPYH
                     GCDGFAIFAKDMDLTLNNPCWNMMKAPWDKAAVAAEQKAA"
     gene            complement(5782..>5964)
                     /gene="nifH"
                     /locus_tag="PLN02_01720"
     CDS             complement(5782..>5964)
                     /gene="nifH"
                     /locus_tag="PLN02_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287173.1"
                     /note="nitrogenase iron protein; nitrogenase component 2;
                     with component 1, an molybdenum-iron protein, catalyzes
                     the fixation of nitrogen to ammonia; nitrogen reductase
                     provides electrons to the nitrogenase complex; in R. etli
                     there are three essentially identical copies of nifH which
                     are actively expressed during symbiosis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase reductase"
                     /protein_id="HOI51067.1"
                     /translation="PKAKQADEYRARASKIVNNTKFVIPTPLEMEELEDLLMEFGIME
                     AEDESIVGKTAAELAA"
BASE COUNT          939 a         1729 c         2005 g         1291 t
ORIGIN      
        1 gtggcaattg ctcttccggc aggcgggcgg cgaccgagag gcggacttcg cggtcggggt
       61 cgtcggccat cgccgccagc tggtcgaccg gcaggcggct ggcgacgacg cggcgaacga
      121 cttcgtcgct atcgctcttg agccggaaga tgcggtcgac cggcgcgtgg cgcgcggcga
      181 tggcgcgacg ttcccagaag atgtcgtcga ggtagtgcgc ggcctcgtcc gggttgcggc
      241 gcaggaaacg gtcgatctgc cggccgctgt gggcacggat gcaggcctcg cccggctggc
      301 agcgacccgc ctcgagaaag gacgggtaca gtgggcaatg ggcgcacagg ccgttggcaa
      361 cggctttctg gtttggcggc tcggccatga tcgcggcctg ttcctggcgg gatgaaaagg
      421 cgggctgccg gacggaccgg caacccatgg tccggctcag gcggcgagcg cttcgtaggt
      481 gaagcagttc ttcgggcaga cccgaccgca ggcgctgcaa ccgatgcagt caccgagatc
      541 cttcagggtc atcttggcgg cggcgctttc gtcctcgtcg tcgtcctcgt cggagtcgat
      601 gaggttgagc acgtcgcgcg ggcagacctt gtagcaacga ccacagccga tgcacttttc
      661 ctcgtcgagg gcggtcacga attgcggaat ccaggggttg ccggcccggg tcagcgcggt
      721 gtagtcagcc atgctacttc ctttctaagc agtgttccgg ccggtggctg ccggccgcga
      781 gtggttgaat gatgtcatct gccagatcgg cggccggttt cttccggaac gtccttcctg
      841 gctcacgcat tcatgatgag caaatttggt gccactcatg aaatcgttgt ttttcaatgg
      901 tttgtcgggt ctttgtggga aatgcgacag gtggcttggc ggcttcgcac gaattcgggg
      961 cgtagatgcg acaacgccgg cctggcgccc tggagggcaa caagccggcg tttgcgcggc
     1021 gtttattcgt cgctgtcgcg gctggcgatg atcacgccgt tggcaaccgc caggtcgctg
     1081 gttgcttcca ggcagtggcc gctgatgccg gtgatcaggt agatcagggc ggccggcgtg
     1141 gtggccaccg cttgcaggcg cggcgagatg tcgtcttcgc tgcactcggt gaagatcagt
     1201 tcgctgaagc tggcgcgcag cgtactggcg ttgtcgccgc gctcgacggc gctcaccact
     1261 tcggccaggc gttcgggggt catctcaggc ctcctcggcg tcgagcgctt cggcgtagcg
     1321 ggcgagcgaa ccgggagcga tgcccatgat ccgcgccagc cagggcggcg ggttgagcag
     1381 ggcgccttgc agttcgccga gcacttcgcg ggcggctccg cccttcggct tcttcagcgg
     1441 atggacgccg gcacgcacga ccttggccgc cgccgggccg ccgatcgatt gcagatagac
     1501 gatctggcaa tcgttgatca ccgccgcccg tgccgcattg cggtcttcgg cggcatcggc
     1561 gtcgaggccg gagcggacgt cgatcaggcg gatttcggtg ggcgagagct ggtagatcag
     1621 gaagttctcg caggagccga agtggccgtc gagattctcg ccgcggttgg cggcgatggc
     1681 gacgcggatc gaaccgggca tgtcaccttc ctgataggcc tccggggtcg gcttgtcgct
     1741 gccggcgacg tcctcgcccc agaggatgcg cacggtttcc ttgagcgctt ccgggctgac
     1801 gccgaccgtg cagttctcgt cggcgtgatc gccggcgagg atttcgcgca agtcctcgac
     1861 ggtcaggcgg gagagcttgc tttcggtgat cggcagctcg agtttttcgg ccagcgcgtt
     1921 gaccagggcg cggacttcca gaccttccag ggccctggcg gccagtgcga tacggtaggc
     1981 ggcttcgcgg gaagcgacgg gttggctcat gactatctcc tggcgtgaaa gcgggtaatg
     2041 gaacgcccgg cgggccggac gctcggttgc cggctctcag gcgggcgaaa tacagcccga
     2101 ggggcagacg ttcacgcaca tcggcgtgtc gttttcgcct tcgcactcgg tgcaggtggc
     2161 ggcgtcgatg gtgtagaaca ccttgccctt cttgatcgcc ttggtcgggc agatcggacg
     2221 gcaatcgtcg caagcggtgc acatatcggg atcgatatac attgccatga tgtttctcct
     2281 gttttcagtc ttccgccgcg cccaggcgcc tggcccgaac cgaaatgggc aggcgggcgg
     2341 gtttggcgat cgggtcaacg tagtagctgc caccgttctc cagcatgagg acgccacccc
     2401 acttttcagc tgtgtcgaac tcgatggatt cgatggcagc ttccaggtcg cgcttgggca
     2461 ggtagaaaac cagcccgcct gcggcattct ggcgaatcat gatgttggcc atgaggcact
     2521 ccggtggcag gaccgggcgg cgggtgccgc ccggtccctg gcggcgggtt agcgaaccag
     2581 gtcgtggttg tagtcggtga cgcccatttc gcgggtctgg tcgtcgaggc gttcgagaac
     2641 cgcgttggtc agggtggtca ggacgtacat cgcgccttcg tagcccagcg tcgtcatccg
     2701 gtgcaggtgg tggcggtcga agatcgggaa gccgatgcgg atcagcggaa cttcgaattc
     2761 cttgccctta tgcacggtgt cgcgctggat gtacttgccg tagctgttgc cgatcaggaa
     2821 gtccggcttg tcggtgaagc acaggctgcg caggtgccag aggtcggcgc ccgggtaggc
     2881 cttgccattg acgccgtagg gcgagctggc gagcagcttt tccatcgcct tcgcccactt
     2941 cttgctgccg ttgttgcaga cgatgtgggt cggctcgatg ccgaattcca gcagcagctt
     3001 gaccatgccc atcaggaagt ccggatcgcc gtagagggcc atcttcttgc cgtgcagcca
     3061 ggcgtgcgag tcggtcatca tgtcgatcag gcggccacgc tccagcttga tggagtcggc
     3121 gatcggcttg ccggtggctt ccgagacctt catcaggaac tcgtcggtcc attccacacc
     3181 catcgggatg ttggtcttcg gcacttcgtg gttccaggtg ttttcgacga acttcttcgt
     3241 cttctcgcag gtcagcggct ggaccaggaa ggtggtgatg gcgttcggcg catccttgac
     3301 ctcgtccatc gtcgtgccgc cggcgtacat gcggaattcg ccgtcagccg gggtgtcgag
     3361 cacttcttcc ggatcgctga gcatcgtgta atcgacgccc atttccttca tgtagcgctt
     3421 gatcacacgg tagttgccga ggtaggtctc gaagcccggc acgatgttga tcttgccgtt
     3481 ggagccgacg accttgcctt ccatggtgtt cagcgtgaag tagcgcagga tcccttcttc
     3541 catgttgtcc cagccggtga catggctgcc gacgaaggac ggggtgtgcg cgaagggaac
     3601 cgggaaatcc ttgtccaggt gaccttcgtt cttcgcgttg ttgatgaagg cgttcaggtc
     3661 gtcgccgatg acttcggcga tacaggtggt cgacatggcg atcatctcgg caccgtagag
     3721 cttggtggcg ttggccaggc cttcatgaac gttcttctga ccgccgaaca cggctgcatc
     3781 ttcagtcatc gagtcggcga tgaaggcgat cggttccttg aagtggcggt tgaagtaggt
     3841 gcggaagtag gccacgcaac cttgcgagcc gtggatgtac accagggact tcttgaagcc
     3901 cagaccgcac agggcggaac cgagcggctg gcaggccttg gccggattga cggtgagcgc
     3961 ttcgcgcttg aagttcagtt cctggtattc ctgggtggtc gtccagttga aaatttccca
     4021 gactttgtcc ttggaatgac cttcctcgaa ctgagccttc ttgttggtca gggtcgcctt
     4081 gtagtcgtca tcgcgaaaca aggggtagca cggcttgatc ttctctacgg tttgcatctt
     4141 gatctccttc gcccgcacca ccaatccgtg gatacgggcg taagtttagg attgccgcct
     4201 gatcaggcag ccttctgttc agctgcgacg gcggccttgt cccacggggc cttcatcatg
     4261 ttccagcacg ggttgttcag cgtcaggtcc atgtccttgg cgaagatggc gaagccgtcg
     4321 cagccgtggt acgggcccga gtagtcccag gagtggagct ggcggaaagg aatgcccatc
     4381 ttgtggaaga cgtacttttc cttgatcccg gagccgatca ggtcgggctt gcaggccttg
     4441 acgaactctt cgaactcgta gccggtgacg tcgtcgtaga tcagggtggc gtcgcccatt
     4501 tccttgatcg tgcggtcgta gtcgtcgttg tgggcgaatt cgtagccggt gccgaccact
     4561 tccatgccca gatcctcgta ggcgccgacc acgtgacgcg gacgcaggcc gccgacgtag
     4621 agcatgacct tcttgccttc caggcgcggc ttgtacttgg cgatcacctc gtccatcatc
     4681 ggcgtgtatt tttcgatgac ggcttcggcg ccagccttga tcttgtcgtc gaagaaggaa
     4741 gcgatcttgc gcagcgattc cttgatcttg gtcgggccga agaagttgta ttccatccag
     4801 ggaatcccgt acttctcttc catgtgccgg ctgatgtagt tcatcgagcg gtagcagtgc
     4861 agcaggttca gcttgacctt cggggtattt tccatttccg ccaaggtgcc gtcgccggac
     4921 cactgggcga ccacgcgcag gcccatttct tcgagcagaa tgcgcgaagc ccaggcgtca
     4981 ccgccgatgt tgtagtcgcc gaggatggca acatcgtagg gggtggtttc gaaggcttga
     5041 ccgtcgcggt tggagaggat ccagtcacgg acggagtcgt tggcgatgtg gtggccgagc
     5101 gattgcgaaa cgccgcggaa gccttcgcag cgcaccggca cgaccggctt gccgatctgg
     5161 gcggcggcct tcttggagac ggcttcgatg tcgtcgccga tgaggccgat cgggcattcc
     5221 gactgaaccg agataccctt gttcagcggg aacaggattt cgatttcctc gatcagcttg
     5281 gccagcttct tgtcaccgcc gaagacgatg tcgcgttcct ggaagtccga ggtgaagttc
     5341 atttcgcaga aggcatcggc gccggtggtg ccgacgtagt agttgcggcg gccggcgcgg
     5401 gcgtactggc cgcagccgat ggggccgtgc gagatcgaga tgatgtcctt gatcggaccc
     5461 cagaccacgc ccttggaacc ggcgtaggca caaccgcgca tggtcatgac gcccggcagg
     5521 gacttgcggt tggaggtgat gcacttcttc gacgactcca gcgtggtgtc gttgaccatc
     5581 aagtgcttgg cccggtcctt cttggccttt tcggggtaaa cctccagcac ctcggcgatg
     5641 agggcggcgg attcttcgcg ggtgagcgct gccatttgaa actcctttcg ttgcgccggc
     5701 atctatccgt gcacggcgcc acagatggtt tggtaagact ttgccgggat gccgccgcag
     5761 cggcgatccc gttggcgact atcaggcggc cagttcggca gcggtcttgc cgacgatcga
     5821 ctcgtcttcc gcttccatga tgccgaattc catcagcagg tcttccagtt cctccatctc
     5881 cagcggcgtg gggatgacga acttggtgtt gttgacgatc ttgctggcgc gggcgcggta
     5941 ctcgtcggcc tgcttggcct tcgg
//
LOCUS       DAOSDX010000046         5914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4827_length_5914_cov_3.030893, whole genome shotgun sequence.
ACCESSION   DAOSDX010000046 DAOSDX010000000
VERSION     DAOSDX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_5914_cov_3.030893"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PLN02_01725"
     CDS             complement(<1..1529)
                     /locus_tag="PLN02_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI51068.1"
                     /translation="MKNNQPVTQNEVRLASDTFIVSRTDLKGSITFVNNTFVEVSGFS
                     REELIGQNHNIVRHPDMPEAAFADLWKTVKAGLPWRGLVKNRCKNGDHYWVEAFVVPI
                     KKDGQPSGYLSIRSPAPADKRAAAEAAYRAAGQQGSLPATGRRTLSLRARIWLAMGAL
                     IVLMATIGLVGLANLKSTDEELNAMYRNNLVPSNLVNRMMFLLSDNRSQIMLALQHDP
                     ANAAAALHDHALDLHIEATLKNRQEINSLLEQLQAIKLSDKETALLQKFGEVRERYSK
                     EGINLARDHLKQGLFMDTNITLLRSVNPLYAEMQREGNALIEEISGAANARYEAALSH
                     YYWVRNFSIGLLLGAILLTLIGGSLLVRGVLGPIRKAIHLFDRIAEGKLTDEIDIDGR
                     DEPGLLLCNLGIMQNTLKAMLDEINSASRAIDTRCRLLENQMRQVSLQSEQQQASVEG
                     VAAATEEFSQSVQEVAANAQETASAASQSQQRVNESNANISQSMAATSRVVDAVAASN
                     R"
     gene            complement(1700..2266)
                     /locus_tag="PLN02_01730"
     CDS             complement(1700..2266)
                     /locus_tag="PLN02_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI51069.1"
                     /translation="MRPIPLILLLASLLSACATGNDPSRSRQIAQSGALKVHPGLLGQ
                     EVPSELQPATPVVAAAETGEKPMRMDEEGLRTQRSVYFDLDQAAIKADYDPILQAHAR
                     YLASHPQTRVRIEGHADERGSSGYNERLGLKRAQSVRATLLGHGAGSTQVTVKTWGKR
                     KPKLLGRNEEAWAENRRADVIYEQDAGK"
     gene            2401..3648
                     /gene="hemA"
                     /locus_tag="PLN02_01735"
     CDS             2401..3648
                     /gene="hemA"
                     /locus_tag="PLN02_01735"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOI51070.1"
                     /translation="MIFTLGINHHSAPLAIRERVAFGADKLQHALAELAQAKAVKEVA
                     ILSTCNRTEIYCAAESAQQVIDWLTSYHQVAYEELAPYLYVHDQPAAVRHAFRVACGL
                     DSMVIGEPQILGQVKDAVRAAEDVGTLGSQLHKLFQRAFSVAKEVRSTTAIGANIVSM
                     AAAGVHLAERIFDSVAGQRILFIGAGEMIELCAAHFCAEKPRQVTVANRTVERGRELA
                     ERYAGRAIRLDELADALPQHDIVVSCTASPLPIIGLGMAERAVKLRRHRPIFIVDLAV
                     PRDVEEEVGELDDVFLYTVDDLAQIVESGMESRQAAVVEAEGIIAQRVQEFIGWLEAR
                     DTVPVIRALRDSAERMRRHEVEHALKLLARGEAPEKVVEQLAQRLSNKFLHAPTSALG
                     AAEGEDRQRLQQTVARLFHLHGD"
     gene            complement(3663..5291)
                     /locus_tag="PLN02_01740"
     CDS             complement(3663..5291)
                     /locus_tag="PLN02_01740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI51071.1"
                     /translation="MSNFPNSVLALAATLERGWASYREQGVFDIFVEFTLSLNGLTEQ
                     LHRLQLPGLVRACQELENTALSFYGERDTHPISLDQAVAIERKLSVILSELRRHETPA
                     IQARRVLDNSEGKAAETWNKPRRVVLVSRAGHPWSTSLSEQLGFFGFKPESIRWEEQP
                     TDDEPPLAIIFIPDVEGRAYPPAAITAIIALRTRFPTSYFYCLSVPSNLQSIVLLQRA
                     GADACVPVGNKVSDVLSRILDLVDMHEQEMYRVLIVEDSATAVAHIRRSLTQHGIDSK
                     AINDPRLLLEVASEYRPDAILMDMYMPFCTGVEVTSALRQIPEYQSLPVIYLSSENDI
                     VQQVEALRLGGDQFLTKPVNPIILASVVKTKIERYREMLYSGQHDSLTGLFNHSTSKL
                     QVERMLNAAQAGGQLVVAMIDIDRFKVINDTYGHPVGDQVIRSLAWLLRGRLRNTDLV
                     GRYGGEEFLIALPDIDPEHAVRLIDQIRLDFGQLPHAHAQGTLRVTFSCGVAALPDYL
                     TTSTLIEAADDALLDAKRSGRNRIVLNRPTSGSD"
     gene            5416..>5914
                     /locus_tag="PLN02_01745"
     CDS             5416..>5914
                     /locus_tag="PLN02_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOI51072.1"
                     /translation="MKASIRQKLDLLVDRLDEIDRLLSSPDAAGDMAQFTRLSRERAE
                     VEPVTIRYNAFRQAESDLAEAEAMRGDPDLREFAEEEIAAVKARLPALEIELQKLLLP
                     KDPNDDKSVILEIRAGTGGDESALFAGDLFRMYARYAERQRWKVEIASQNESELGGYR
                     EIIARI"
BASE COUNT          945 a         1790 c         2034 g         1145 t
ORIGIN      
        1 atccggttgg aggcggcgac ggcatcgacc acccgcgacg tggcggccat gctctggctg
       61 atgttggcgt tgctctcgtt gacccgctgt tgcgactggc tggcggcgct ggcggtttcc
      121 tgggcgttgg ccgccacttc ctggaccgac tggctgaact cctcggtcgc cgccgcgacg
      181 ccctcgacgc tcgcctgttg ctgctccgat tgcagcgaca cctggcgcat ctggttttcc
      241 agcaggcggc agcgggtgtc gatggcgcgc gaggcactgt tgatctcgtc aagcatcgcc
      301 ttcagcgtgt tctgcatgat gccgaggttg cacaagagca ggccgggctc gtcgcgaccg
      361 tcgatgtcga tctcgtcggt cagcttgcct tcggcgatcc ggtcgaacag gtggatcgcc
      421 ttgcggatcg ggccgagcac accgcgcacc agcagcgagc cgccgatcag cgtcagcagg
      481 atggcgccga gcagcaggcc gatgctgaag ttgcgcaccc agtagtagtg gctcaatgcg
      541 gcttcgtaac gagcgttggc ggcacccgat atttcctcga tcagggcatt gccttcccgt
      601 tgcatctcgg cgtagagcgg gttcacgctg cgcagcaggg tgatgttggt gtccatgaac
      661 aagccctgct tgagatggtc gcgggcgaga ttgatgccct ccttggagta gcgttcgcgc
      721 acttcgccga atttctgcag cagcgccgtt tccttgtcgc tgagtttgat ggcctgcagt
      781 tgttcgagca ggctgttgat ctcctggcgg ttcttcaggg tcgcctcgat gtgcaggtcg
      841 agggcatgat cgtgcagtgc cgcagccgcg ttggccgggt cgtgctgcag cgccaacatg
      901 atctgcgagc ggttgtccga aagcaggaac atcattcggt tgaccaggtt cgaaggtacc
      961 aggttgttgc ggtacatcgc gttcagttct tcgtcggtgc ttttcaggtt ggcgagcccg
     1021 accaggccga tcgtcgccat cagcacgatc agtgccccca tcgccagcca gatgcgggcg
     1081 cgcaggctca gcgtccggcg gccagtggcg ggcagcgaac cttgctggcc ggcagcccgg
     1141 taggcggcct cggcggcggc tcgtttgtcg gccggggccg gtgagcggat cgacaggtag
     1201 ccgctcggct ggccatcctt cttgatcggc acgacgaagg cttcgaccca gtaatgatcg
     1261 ccgttcttgc agcggttctt gaccaggccg cgccaaggca agccggcctt gaccgtcttc
     1321 cacaggtcgg cgaaggcggc ttccggcatg tccgggtggc ggacgatgtt gtgattctgg
     1381 ccgatcagtt cctcgcggct gaagccgctg acttcgacaa aggtgttgtt gacgaaggtg
     1441 atactgcctt tgaggtcggt gcgggagacg atgaaggtat cgctcgccag acgcacctcg
     1501 ttctgcgtga caggctggtt gtttttcatg ttcgctccag ggggcaagcg ttgctggccg
     1561 gacttttccg ccgcatttcc tcatccgctg cggttgccgt ttgggggcga atatataact
     1621 ttagttctag ttcgtgtggg gctttgcctt cccgaaacac tgtgcaaaaa aaacccggcg
     1681 ggcgccgggt tggtttgttt tatttgccag catcctgctc gtagatcacg tcggcccgtc
     1741 ggttctccgc ccaggcttcc tcgttgcgtc cgagcagctt cggcttgcgc ttgccccagg
     1801 tcttgaccgt gacctgggtg ctgccggcgc cgtggccgag cagcgtggcg cgcacgcttt
     1861 gcgcccgctt caggccgagg cgttcgttgt agccgctgct gccgcgctcg tcggcgtggc
     1921 cttcgatgcg tacccgggtt tgcggatggc tcgccaggta gcgggcgtgt gcctgcagga
     1981 tgggatcgta gtcggccttg atcgctgcct gatcgaggtc gaaatagacg ctgcgctgcg
     2041 tacgcaggcc ttcttcgtcc atgcgcatcg gcttttcgcc ggtttccgcg gcggccacca
     2101 ccggcgttgc cggttgcagt tcggacggca cttcctggcc gagcagcccg gggtgcacct
     2161 tgagcgctcc gctctgcgcg atctgccggg agcgtgacgg atcgttgccg gtggcgcagg
     2221 cgctgagtag ggaggcaagc aacaggatga ggggaatcgg gcgcatgatg gtcgggcaac
     2281 taagcaatcg caggatgata gccgctctcg gcccgctcta ccagtttccc ttgcgcccgg
     2341 gaaggtgggg tttcggggcg gtgtataatc gcgcaccaaa tttcgttacg ccagtttccc
     2401 gtgatcttca ccttaggcat caaccaccac tcggcaccgc tcgccatccg cgaacgcgtg
     2461 gcttttggcg ccgacaagct gcagcatgcg ctggccgaac tggcgcaggc caaggcggtg
     2521 aaggaggtgg cgatcctgtc gacctgcaac cggaccgaga tctactgtgc cgcggaatcg
     2581 gcgcagcagg tgatcgactg gctgacgtcc taccaccagg tggcgtacga ggaactggcg
     2641 ccctatctct acgtgcatga ccagccggct gccgtgcgtc atgccttccg ggttgcctgc
     2701 ggacttgatt cgatggtcat cggcgagccg cagattcttg gtcaggtcaa ggacgcggtg
     2761 cgcgccgcgg aagatgtcgg cactctcggc agccagttgc acaagctgtt tcagcgcgcc
     2821 ttctcggtgg ccaaggaagt gcgcagcacc accgcgatcg gcgccaacat cgtgtcgatg
     2881 gcggcggctg gggtccatct cgccgagcgc atcttcgatt cggtggccgg tcagcgcatc
     2941 ctgttcatcg gcgccggcga gatgatcgaa ttgtgcgccg cccacttctg tgccgaaaag
     3001 ccgcgtcagg tgacggttgc caaccgcacc gtcgaacgtg gccgggagct ggccgaacgc
     3061 tacgccggtc gcgccatccg cctcgacgag ctggccgatg ccttgccgca gcacgacatc
     3121 gtcgtttcct gtaccgccag tccgctgccc atcatcggcc tggggatggc cgagcgggcg
     3181 gtcaagctgc gccgtcaccg gccgatcttc atcgtcgacc tggcggtgcc gcgcgacgtc
     3241 gaggaagagg tcggcgaact cgacgacgtc ttcctgtaca ccgtcgacga cctagctcag
     3301 atcgtcgaga gcggcatgga gtcgcgccag gcggcggtgg tcgaggccga gggaatcatt
     3361 gcccagcggg tgcaggaatt catcggctgg ctcgaggcgc gcgacacggt gccggtgatc
     3421 cgcgccctgc gcgattcggc cgagcgcatg cggcgccacg aagtcgagca cgcgctgaag
     3481 ttgctggcgc gtggcgaggc gccggagaag gtggtcgaac aactggcgca gcgcctgagc
     3541 aacaagttcc tgcacgcgcc gaccagcgcc ctcggcgcgg ccgaaggcga ggacaggcaa
     3601 cgcctgcagc agacggtagc gcgtctgttc cacctgcacg gcgactgagc gccagggggg
     3661 cttcagtcgc tgccgctcgt cgggcggttg aggacgatgc ggttgcggcc gctgcgcttg
     3721 gcgtcaagca gcgcgtcgtc ggcggcttcg atcagggtcg aggtggtcag gtaatcgggt
     3781 agcgcggcga cgccgcaact gaaggtgacg cgcaaggtgc cctgggcgtg ggcgtgcggt
     3841 agctggccga aatcgagacg gatctggtcg atcaggcgga ccgcgtgctc cgggtcgatg
     3901 tcgggcagcg caatcaggaa ttcctcgcca ccgtagcggc cgaccaggtc ggtgttgcgc
     3961 aggcggccac ggagcagcca ggccaggctg cggatcacct ggtcgccgac cgggtggccg
     4021 taggtgtcgt tgatcacctt gaagcggtcg atgtcgatca tcgccaccac caactggccg
     4081 ccggcctgcg cggcattgag catgcgctcg acctggagct tcgaggtcga atggttgaac
     4141 agcccggtca ggctgtcgtg ctggccggaa tagagcattt cccggtagcg ctcgatcttg
     4201 gtcttgacca ccgaggcgag gatgatcggg ttgaccggct tggtcaggaa ctggtcgccg
     4261 cccaggcgca gggcttcgac ctgctgcacg atgtcgttct cgctcgacag gtagatcacc
     4321 ggcagcgact ggtattcggg aatctggcgc agcgcgctgg tgacttcgac gccagtgcag
     4381 aagggcatgt acatgtccat caggatggcg tcagggcggt attcgctggc cacttcgagg
     4441 agcaggcgcg ggtcgttgat cgccttgctg tcgatgccgt gctgggtcag cgagcgccgg
     4501 atgtgggcga cggcggtggc cgagtcctcg acgatcagca cgcggtacat ctcctgctcg
     4561 tgcatgtcca ccagatcgag gatacgcgac aggacgtcgc tgaccttgtt gccgaccggc
     4621 acgcaggcgt cggcgccggc gcgctgcagc aggacgatgc tttgcaggtt ggagggtacc
     4681 gacaggcagt agaaatagct ggtcgggaag cgcgtgcgca gggcgatgat cgcggtgatc
     4741 gccgccggcg gataggcccg gccttcgacg tcggggatga agatgatggc gagcggcggc
     4801 tcgtcgtcgg tgggttgttc ttcccagcgg atgctttccg gcttgaagcc gaagaaaccg
     4861 agctgttccg acagcgaggt ggaccagggg tggccggcgc ggctgacgag aaccacgcgg
     4921 cgcggcttgt tccaggtctc cgcggctttc ccctcgctgt tgtcgaggac gcgccgggcc
     4981 tggatggccg gtgtttcgtg gcgccgcagt tcgctcagga tgacgctcag cttgcgctcg
     5041 atggcgaccg cctggtcgag cgagatcggg tgggtgtcgc gttcgccgta gaaggacagc
     5101 gcggtgtttt ccagttcctg gcaggcgcgg accagccccg gcaactgcag gcgatgcagt
     5161 tgttcggtga ggccgttcag cgatagggtg aattcgacga agatgtcgaa cacgccctgt
     5221 tcgcggtaac tggcccagcc tcgctcaagg gtggcggcga gggcgagcac ggaatttgga
     5281 aaatttgaca tcgcgcattc agaacaatcg aacaattatg ctgacggaac ccgatcctgg
     5341 caatctcggt gtattaccag gggggaactc gggtaccata acggccccat tctcctagtc
     5401 ccgccgcccg gatcgatgaa agctagcatt cgtcaaaaac tggatctgct ggtcgatcgc
     5461 ctcgacgaaa tcgatcgtct gctgtcttcg cccgacgcgg ctggcgacat ggcccagttt
     5521 acccgtctct cgcgcgagcg ggcggaagtc gaaccggtga ccattcgcta caacgccttt
     5581 cgtcaggcgg aaagcgatct ggcggaagcg gaggcgatgc gcggcgatcc cgatctgcgc
     5641 gagttcgccg aagaggaaat tgccgccgtc aaggcgcgcc tgccggcact cgaaatcgaa
     5701 ctgcagaaac tgctgctgcc caaggatccc aacgacgaca agagcgtcat cctggaaatc
     5761 cgcgccggca ccggcggcga cgagtcggcg ctgttcgccg gcgacctgtt ccgcatgtac
     5821 gcgcgctacg ccgagcgcca gcgctggaag gtcgagatcg cctcgcagaa cgagtccgag
     5881 ctcggcggct atcgcgagat catcgcccgc atcg
//
LOCUS       DAOSDX010000047         5884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4850_length_5884_cov_3.802539, whole genome shotgun sequence.
ACCESSION   DAOSDX010000047 DAOSDX010000000
VERSION     DAOSDX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4850_length_5884_cov_3.802539"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2025)
                     /locus_tag="PLN02_01750"
     CDS             complement(<1..2025)
                     /locus_tag="PLN02_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI51073.1"
                     /translation="MSELPFLVWALVALGSGLLIHQRWRIAGLQAEQGRYQAIFDNAM
                     VGMANSTPALRWTDANPTLCQILDQSVANLTAVDWLDFVHPEDRPVTQRLLDAIRHGE
                     RATGTLEVRCLRRDGSIVFVNLGMQAIRGDSGELDSLFMMIEDITQRQVAETQFANQV
                     NRSLVLIELPRQAELLDEREFMRYALERAEALTGSRIGFMHFVNDDGENIELAIWTRK
                     TVEHYCTAVADRHYPISQAGIWADAARTGQAMMVNDYAAAENKRGLPEGHSQLTRLLS
                     VPVVENGVVRMLAGVGNRESAYAAYDSETVQLIANETWRIVRRLRADRALRLAMQVVN
                     ASPVVCFRWAATTGWPVVFVSENVRQWGYTQEQLQSGTPSFSSIIHPDDLARVCDEVA
                     RHTAAGLNAYEQEYRLITAQGEVIWVVDRTKVSRDAQDQPTYYDGVITDISERKQQQL
                     LIADALAQQRVLNKRLEEANNQLLQSEKMASIGQLAAGVAHELNNPIGFVHSNLGTLD
                     GYIRDLMAIIDAYAAKSGDDYPHQKEMQLLREERDFDFVREDIFKLLAESKDGLARVR
                     KIVQDLRTFSRVGEQEWQEADLHEGLDSTLNIVWNELKYKCEVVKDYGDLPPVYCLIS
                     QLNQVFMNLLVNAGHAIAVKGTITIRTRQLDEARVSVEISDTGCGIAQENL"
     gene            complement(2022..4979)
                     /locus_tag="PLN02_01755"
     CDS             complement(2022..4979)
                     /locus_tag="PLN02_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI51074.1"
                     /translation="MADLAASDAKTPRPVVLAVDDMKANLAIVVAMLEDLEIEVRCAE
                     SGEAALRAAVEPPQPDLILLDVMMPGMDGHAVLARLRADPATSQIPVIFVTALNSPND
                     EQSGLLEGAVDYITKPLTASTLRARVKNHLELKWARDRLARHNANLEIEVQRRLLENQ
                     DLAARLQLTLSASSLGIWEYHHDDDRLELNPDLCRMLGLERTPGSMAAALPLIHPDDS
                     KLVLAAIRKPPPPGGAAQIREFRLRHADGRWLWVESRGQALYRDADGAPLVSAGTIAD
                     ITRRKEIEIERQLTSVVFTGINDGICIADPASRILSVNEAFCRITGYSAEEAIGQTTA
                     ILRSGKHGPAFYQSLWQSLEQCGGWQGEITNRRKNGELYSAWLSVSAIRDPHGRVTHY
                     VGLISDISERLQAAERIQYLSSFDPLTDLPNRALLSDRLAQALHNASRMARQTAILLL
                     DLDRFRVINESFGALFGDALLQEVAQRLKTLVRDGDTVGRRSGAEFGFVMVNLEHESD
                     IIALAQRLLDALSLPFTINGQSVSLNACIGISLSPRDGGTVDELMKTADVALSRAKHA
                     GSNVFRFYSPEMDADAARRLALESALRSALENCELAVYYQPQVSLDSGRLIGMEALLR
                     WHSPQFGTVSPGEFIPLAEDNGLIVTIGEWVLKTACRQARRWLDLGIGSPLRLAVNLS
                     ARQFRQADLTRMVGHALSNSGLPAAALELEITESAFIDDVDQAIAMCHDLKKLGVKLS
                     LDDFGTGYSSLSYVSRFPFDKIKLDQSFVRDIIENPVNAAIATAAIVMARSLNLAILA
                     EGVETEAQARFLRGRRCDAIQGYLFAPALPVNEFEQLLTGRQTLPIADLPSVGENTLL
                     IVDDEPNILAALSRLLRREGFHILTAETPSAAFEHLAKHPVQVVLSDQRMPEMSGSEF
                     LSRVRQLYPDTVRIVLSGYTDLDSVTSAINQGAIYKFLTKPWDDDALREEIRDAFRLV
                     KERRQHFATSHQP"
     gene            5133..>5884
                     /locus_tag="PLN02_01760"
     CDS             5133..>5884
                     /locus_tag="PLN02_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOI51075.1"
                     /translation="MLKKLDSFAGVAGPLVVVVMDGYGLPENPVGSAIAEARQPNLDR
                     LFASSPHIRLRAHGTAVGMPSDDDMGNSEVGHNAIGAGQVYAQGAALVANAIAEGTIW
                     AGQAWQEIVGAAQDGVLHFIGLFSDGNVHSHIDHLRAMVERAKAEGVKTVRIHVLLDG
                     RDVPETSALDYVVPFEAFLAGISDAGFDARIASGGGRQNITMDRYDANWPMVERGWRT
                     HVLGEGPQFASASAAVEALRAQYPGTIDQDLPP"
BASE COUNT          926 a         1766 c         2076 g         1116 t
ORIGIN      
        1 gaggttttcc tgggcgatgc cgcagccggt gtcgctgatc tcgacgctga cccgtgcctc
       61 gtcgagttgc cgggtgcgga tggtgatcgt gcccttgacg gcgatcgcgt ggccggcatt
      121 gaccagcagg ttcatgaata cctggttgag ttgcgagatc aggcaataga ccggcggcag
      181 gtcgccgtag tccttgacca cctcgcactt gtatttgagt tcgttccaga cgatgttgag
      241 cgtcgaatcg agcccttcgt gcaggtcggc ttcctgccat tcctgttcgc cgacgcgcga
      301 gaaagtgcgc aggtcctgga cgatcttgcg cacgcgggcg aggccgtcct tggattccgc
      361 caacagcttg aagatgtctt cacggacgaa atcgaaatcg cgctcctccc gcagcaactg
      421 catttccttc tggtgcgggt agtcgtcgcc gcttttcgcg gcgtaagcgt cgatgattgc
      481 catcaggtcg cggatgtagc cgtcgagggt gccgaggttg gagtgcacga agccgatcgg
      541 gttgttcagt tcgtgggcga cgccggccgc cagttggccg atcgaggcca ttttttcgga
      601 ttgcagcaac tggttgttgg cttcctccag gcgcttgttg agaacccgtt gctgcgccag
      661 ggcgtcggcg atcaacagtt gctgctgctt gcgttcgctg atgtcggtga tcacaccgtc
      721 atagtacgtc ggctgatcct gcgcgtcacg actgaccttg gtccggtcga ccacccagat
      781 cacctcgccc tgggcggtga tcaagcggta ttcctgctcg taggcgttga gcccggcggc
      841 ggtgtggcgg gcgacttcgt cgcagacccg agcgagatcg tcggggtgga tgatgctcga
      901 gaaggagggg gtgccggact gcaactgctc ctgggtatag ccccactggc ggacgttttc
      961 ggaaacgaag acgaccggcc agccagtggt cgccgcccag cggaaacaaa ccaccgggct
     1021 cgcgttgacc acctgcatgg ccaggcgcaa ggcccggtcc gcccgcaggc gacggacgat
     1081 gcgccaggtt tcgttggcga tcaactgcac cgtctcgctg tcgtaagccg cgtaggcgct
     1141 ttcccggttg ccgacgccgg cgagcatgcg gacgacacca ttttcgacca ccggcacgct
     1201 gagcaggcga gtcagttgcg aatggccttc cggcaggccg cgcttgtttt cggcggcggc
     1261 gtaatcgttg accatcatcg cctgtccggt gcgggcggcg tcggcccaga tgcctgcctg
     1321 gctgatcggg tagtggcgat cggcgactgc cgtgcagtag tgctcgaccg ttttgcgggt
     1381 ccagatcgcc agttcgatgt tttcgccgtc gtcgttaacg aaatgcatga agccgatccg
     1441 gctgccggtc agcgcctcgg ctcgctccag cgcgtagcgc atgaattccc gctcgtcgag
     1501 gagttcggcc tggcgcggca gttcgatcag gaccagcgaa cggttcacct ggttggcgaa
     1561 ctgcgtttcc gcgacctggc gttgggtgat gtcctcgatc atcatgaaca gcgagtcgag
     1621 ttcgccgctg tcgccacgga tcgcctgcat cccgagattg acgaagacga tgctgccgtc
     1681 acggcgcagg cagcgcactt cgagcgtgcc ggtagcgcgt tcgccgtggc ggatggcgtc
     1741 gagcaggcgt tgggtgaccg ggcggtcttc cgggtgcacg aagtcgagcc agtcgacggc
     1801 ggtcaggttt gccacgcttt ggtcgaggat ctggcaaagg gtcgggttgg cgtcggtcca
     1861 gcgcaacgcc ggggtcgagt tggccatgcc gaccatggca ttgtcgaaaa tcgcctggta
     1921 acgcccttgc tctgcctgca ggccggcgat gcgccagcgc tgatggatca gcagtccgct
     1981 accgagggcg acgagcgccc aaaccaggaa gggtagttcg ctcatggctg gtggctggtg
     2041 gcgaagtgct ggcgtctttc cttgaccagg cggaaggcgt cgcggatttc ctcgcgcagc
     2101 gcatcgtcgt cccagggctt ggtcaggaat ttgtagatgg cgccctggtt gatcgcgctg
     2161 gtcacgctgt cgaggtcggt gtagccgctg aggacgatgc gcacggtgtc cgggtaaagc
     2221 tggcggacgc gggagaggaa ttcgctgccc gacatttccg gcatccgctg gtcggagaga
     2281 acaacctgca ccgggtgctt ggccaggtgt tcgaaggcgg cggacggggt ttccgcggtc
     2341 aggatgtgga agccctcgcg gcgcagcagg cgcgacaggg cggcaaggat gttcggttcg
     2401 tcatcaacga tgagcagcgt gttttcgccg accgacggca gatcggcgat cggcagcgtc
     2461 tggcggccgg tcagcagttg ctcgaattcg ttgaccggca gcgccggggc gaacaggtag
     2521 ccttgaatcg cgtcgcaccg gcggccgcgc aggaagcggg cctgggcctc ggtttcgacg
     2581 ccttcggcga ggatcgccag gttcaggctg cgtgccatga cgatggcggc ggtggcgatc
     2641 gccgcgttga ccggattttc gatgatgtcc ctgacgaagc tctggtcgag cttgatcttg
     2701 tcgaagggga agcgcgaaac gtaggacagt gacgaatagc cggtgccgaa gtcgtcgagc
     2761 gacagcttga cgccgagctt cttcaggtcg tggcacatgg cgatcgcctg gtcgacatcg
     2821 tcgatgaagg cgctttcggt gatttccagc tccagcgcgg cggccggcag gccgctgttg
     2881 ctgagggcgt ggccgaccat gcgggtcagg tcggcctggc ggaactggcg agccgacagg
     2941 ttgaccgcca gccgcagcgg tgagccgatg cccaggtcga gccagcgcct ggcctggcgg
     3001 caggcggttt tcaacaccca ttcgccgatc gtcacgatca gaccgttgtc ttcggcgagc
     3061 gggatgaact cgccgggcga gaccgtgccg aactgtgggc tgtgccagcg cagcaaggct
     3121 tccatgccga tcagccggcc gctgtcgaga ctgacctggg gctggtagta aaccgccagc
     3181 tcgcagttct ccagcgcgct gcgcagcgcg ctttccaggg ccaggcggcg ggcggcgtcg
     3241 gcatccattt ccggcgaata gaagcggaag acgttgctgc cggcgtgctt ggcgcgcgac
     3301 agcgcgacgt cggcggtctt catcagttcg tcgaccgtgc cgccgtcgcg cgggctgagg
     3361 ctgatgccga tgcaggcatt gagcgagacc gactggccgt tgatcgtgaa gggcaggctg
     3421 agagcgtcga gcaggcgctg ggcgagggcg atgatgtcgc tctcgtgctc caggttgacc
     3481 atcacgaaac cgaactcggc gccggaccgg cggccgacgg tgtcgccgtc gcgcaccagc
     3541 gtcttcagcc gctgggcgac ttcctgcagc agtgcgtcgc cgaacagggc gccgaagctc
     3601 tcgttgatca cccggaagcg gtcgaggtcg agcagcagga tcgccgtctg gcgcgccatg
     3661 cggctcgcgt tgtgcagtgc ctgggcgagg cggtcgctga gcagggcgcg gttcggcagg
     3721 tcggtcagcg ggtcgaaact ggacagatac tggatgcgtt cggccgcctg caggcgttcg
     3781 gagatatcgc tgatcaggcc gacgtaatgg gtgacgcggc catgcgggtc gcggatcgcc
     3841 gaaacgctca gccaggccga atagagctcc ccgttcttgc gccggttggt gatctcgccc
     3901 tgccagccgc cgcattgctc caggctctgc cacaagcttt ggtagaaggc cgggccgtgt
     3961 ttgccggaac gcaggatggc ggtggtctgg ccgatcgctt cctcggcgct gtaaccggtg
     4021 atgcggcaga aagcttcgtt gacgctgagg atgcggctgg ccgggtcggc gatgcagatg
     4081 ccgtcgttga tgccggtgaa gacgaccgag gtcagttggc gctcgatctc gatttccttg
     4141 cgccgggtga tgtcggcgat ggtgccggcg ctgaccagcg gggcgccatc cgcatcacgg
     4201 tagagggcct ggccgcggct ttcgacccag agccagcggc cgtcggcgtg gcgcagccgg
     4261 aattcgcgga tctgcgcggc gccgcccggc gggggcggct tgcggatggc ggccaggacc
     4321 agcttgctgt cgtccgggtg gatcagcggc agcgcggcgg ccattgagcc cggcgtcctt
     4381 tcgaggccga gcatgcggca gagatcggga ttcaactcaa ggcggtcgtc gtcgtggtgg
     4441 tattcccaga tcccgagact ggaggcggac agggtcagct gcaggcgggc ggcgaggtcc
     4501 tggttttcca gcaggcggcg ctgcacctcg atttccaggt tggcattgtg tcgggccagg
     4561 cggtcgcgtg cccacttcag ttcgaggtgg ttcttgaccc gggcgcgcaa ggtcgaagcg
     4621 gtcagcggct tggtgatgta atcgacggcg ccttcgagca gtccgctctg ttcatcgttg
     4681 ggcgagttga gggcggtgac gaagatcacc gggatctggc tggtggcggg gtcggcacgc
     4741 aggcgggcga gcacggcatg gccgtccatc cccggcatca tgacgtcgag caggatgagg
     4801 tcgggctggg ggggctcaac ggcggcacgc agcgcggctt ccccactttc ggcgcagcgg
     4861 acctcaatct cgaggtcttc gagcatggcc acgacgatgg ccaggtttgc cttcatgtca
     4921 tcgacggcaa gcactaccgg acggggtgtc ttggcatcgg aggcagcaag gtcagccatc
     4981 gtgtttcctc ttacatttgt tattgttgtc attttggact gggccagccg ttcaggcaag
     5041 cgtcaagcgt taaaataccg caactccatt tttctgggcg gattgtccag gcgccggcgg
     5101 cgttttcgcc ccgttttttt gtagggaacc cgatgctgaa gaaactcgac tcctttgccg
     5161 gcgtggccgg tccgctcgtc gtcgtcgtca tggatggcta cggtctgccg gaaaaccccg
     5221 tcggcagtgc catcgccgaa gcgcgccagc ccaatctcga ccgcctgttc gcgagcagcc
     5281 cgcacatccg cttgcgcgcg cacggcaccg cggtcggcat gccgtccgac gacgacatgg
     5341 gcaattcgga agtcggccac aacgcgatcg gtgccggtca ggtctatgcg cagggggcgg
     5401 cgctggtcgc caacgcgatc gccgaaggca cgatctgggc cggtcaggcc tggcaggaaa
     5461 ttgtcggtgc ggcgcaggac ggcgttctcc atttcatcgg tctcttttcc gacggtaacg
     5521 tgcatagcca cattgaccac ctgcgggcga tggtcgagcg ggccaaggcg gaaggggtga
     5581 agacggtacg catccacgtc ctgctagacg gccgcgacgt gccggaaacc agcgccctcg
     5641 actacgtcgt gccgttcgag gccttcctcg ccggcatcag cgatgccggc ttcgacgccc
     5701 gcatcgcttc cggcggtggc cgccagaaca tcacgatgga ccgctacgac gccaactggc
     5761 cgatggtcga acgcggctgg cgcacccacg tgctcggcga aggaccgcag ttcgccagtg
     5821 ccagcgcggc ggtcgaagcc ttgcgcgcgc agtatcccgg caccatcgac caggacctgc
     5881 cgcc
//
LOCUS       DAOSDX010000048         5820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4910_length_5820_cov_3.337554, whole genome shotgun sequence.
ACCESSION   DAOSDX010000048 DAOSDX010000000
VERSION     DAOSDX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4910_length_5820_cov_3.337554"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..999
                     /gene="cbiB"
                     /locus_tag="PLN02_01765"
     CDS             112..999
                     /gene="cbiB"
                     /locus_tag="PLN02_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOI51076.1"
                     /translation="MPLAALAAVLLDRLFGEVRRFHPLVGFGTLAAAIERRLNRRTIA
                     GGVLGWALAVLPWVVLALALRPLAPFAVDVLLLYFSLGARSLCEHAEAVARPLREGRL
                     DEARQRVGWIVSRDTATLDESGVAKAGVESVLENGNDAIFGALFWFALLGGPGALLFR
                     LANTLDAMWGYRTERYNLYGRWAARCDDALNWLPARLTALSYALLGRWRDALTCWRAQ
                     APGWESPNAGPVMAAGAGSLGVQCGGAAIYHGREEIRPPLGRGPAPVAADLGRAITLI
                     RRSLWLWLAVLFLIGALHA"
     gene            992..1984
                     /gene="cobD"
                     /locus_tag="PLN02_01770"
     CDS             992..1984
                     /gene="cobD"
                     /locus_tag="PLN02_01770"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HOI51077.1"
                     /translation="MLDHGGRLREAAAHYDIPLADWLDLSTGINPQGWPVPPLPAAAW
                     QRLPEDNDGLEAAAAAYYGNANLLPVAGSQAAIQWLPALLPRAAVACIAPLYNEHPQA
                     WQRAGHKLRFLQNATLPRALAAATPYVLLCNPNNPTADRHPRELAVDAAAQLKKRGGW
                     LIVDEAFVDATPEHSLTPLAGTADAPNLIVFRSLGKFFGLAGLRVGFLFAAPELLAKM
                     AEAIGPWSIAGPSRAAATLALADRAWQAATRERLQADGERLRQLLAPYGELKATPLFA
                     TLNAPATAELHEHLARRAILSRRYESNPLLRVGLPPDEAGWQRLADALKAWKPA"
     gene            1981..2862
                     /locus_tag="PLN02_01775"
     CDS             1981..2862
                     /locus_tag="PLN02_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOI51078.1"
                     /translation="MKRRLFALLAAACLGGAHAEIVVRDDSGRDVHLREPARRIVALA
                     PHIAETLFAAGAGGKLVGTVDYSDYPPAAKKVPRVGGYSRIDLEAVVALKPDVVLVWE
                     SGNNMAQADKLRALGLTVFVSQPNRMDDIAGQVERYGRLAGTEAIANAEAHRFRERLA
                     ALRAANAGKPKVRTFYQVWKAPLVTVGGPQIISDAMRICGGENVFGQLDQMAPRVSLE
                     AVIEADPEAIVATGMGDAKPEWLDDWRQWKRMTAVQRGNLFHINPDIMQRHTPRILDG
                     TAKLCDHLDVARSRRPK"
     gene            2870..3670
                     /locus_tag="PLN02_01780"
     CDS             2870..3670
                     /locus_tag="PLN02_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOI51079.1"
                     /translation="MSRLPQRIVCLTTETVEVLYALGEQERIVGISGYTVRPPQARKE
                     KPKVFAFTSGDIAKILAVEPDLVLTFSDLQADIARDLIKAGIPVYALNMRSVDDILAM
                     IETLGRLVGAEDRALALVADLEAQIAAARAHAEASIARLGRRPRVYFEEWDEPLISGL
                     RWVSELIEIAGGEDVFAERARSPLAADRRPTPEEVIAAAPDIIIGSWCGKHFRPERVA
                     ARPGWEAIPAVKQGRLHEIKSAIVLTPGPVAISEGLPLLEKILWPASA"
     gene            3720..4613
                     /locus_tag="PLN02_01785"
     CDS             3720..4613
                     /locus_tag="PLN02_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOI51080.1"
                     /translation="MLRSALILLIFSLFTLLARAGEIAIVLADSSAPYQEFASGLQQR
                     LKGSNWQISYVDTEPHAPERQPVALIVTAGSEALRKTLASSPKVPVLATLLTEHAYQN
                     QIGTSRPPAVSAIYLDQPAQRQARLLRLLFPELQRVGLLLSEQSRQQADSLRTALAGQ
                     RLQPVSEFANGEEYILPTLEALMQRSEVLLALPDPLLYSRNTIKPILITSYRHRRPVI
                     AFSPAMVKAGALAALYSTPLQIGRQAAELLIAHGTHLPLPATPSKFSIDINRSVADAF
                     GLRLPEESDLFQKLLASEVNP"
     gene            4610..>5820
                     /locus_tag="PLN02_01790"
     CDS             4610..>5820
                     /locus_tag="PLN02_01790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF021499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI51081.1"
                     /translation="MKNLTLRQRLLVLTLLPSALITTLLVVYFAITGINTLENQLRAK
                     GLATVRYLAPISEYGIIAGQMDSVYGLVQAAMQEPGIKAAIIVNPKGRTLAVSGRVSL
                     SAETIRQRLNAPALVGEGDTWIAFGAPVVRSQSDSDPLFDIAGLDENPQETIGHVFIE
                     LDKEELQVRQQELVLQGFIAVLCGLGLMGALAIAMADHLARPLQRLAAAVRNMSAGRF
                     DTRVQASSSGELRVLEDGFNEMASHIEEAHQSMQTRIEEATAQLVFQARHDALTGLVN
                     RREFELRLDKALAAVHAGGDEFSVLYIDLDRFKAVNDACGHIAGDELLRQIALLFQGR
                     LREEDTLARLGGDEFGIMLANCSLAKARQVAQDICALSAEYRFVWQDKIFAIGASIGL
                     TPVTRDVRDVH"
BASE COUNT          966 a         2137 c         1831 g          886 t
ORIGIN      
        1 gatcggctcg acctgctggc acaacagtcc acaagcgcgc gcggcgatcg acgccgccat
       61 cgccctggcc cgccaatggc cgaactgaac ctgctcgccg gcccctacgc catgccgctg
      121 gcagcgctcg ccgcggtgct gctcgaccgc ctgttcggcg aagtgcgacg ctttcacccg
      181 ctggtcggct tcggcacgct ggcggcggcg atcgaaagac ggctgaaccg gcgcacgatt
      241 gccggcggtg tcctcggctg ggcgctggcg gtgctgccct gggtggtgct ggcgctggcc
      301 ttgcggccat tggcaccgtt cgcggtcgac gtgctgctgc tctatttttc gctcggcgcg
      361 cgcagcctgt gcgagcacgc cgaagcggtc gcccggccgc tgcgcgaggg gcggctcgac
      421 gaagcgcggc agcgcgtcgg ctggatcgtt tcccgcgata ccgcaacact cgacgagtcc
      481 ggcgtcgcca aggccggcgt cgaatcggtg ctggaaaacg gcaacgacgc gatcttcggc
      541 gcgctgttct ggttcgccct gctcggcggc cccggcgccc tgcttttccg gctggccaac
      601 acgctcgacg cgatgtgggg ctaccgcacc gaacgctaca acctgtacgg ccgctgggcc
      661 gcccgctgtg acgacgcgct caactggctg ccggcccgac tgaccgcgct gagctacgcc
      721 ctgctcggcc gctggcgcga cgcgctcact tgctggcgcg cccaggcgcc cggctgggag
      781 agcccgaacg ccggcccggt gatggcggcg ggcgccggca gcctcggtgt gcaatgcggc
      841 ggcgccgcca tctaccacgg ccgcgaggaa atccgcccgc cgctcggccg cggcccggca
      901 ccggtcgccg ccgacctcgg ccgggcgatc acgctgatcc ggcgcagcct gtggctgtgg
      961 ctggctgtcc ttttcctgat cggagccctg catgcttgac catggcggcc gcctgcgcga
     1021 agccgcggcc cactacgaca tcccgctcgc cgactggctc gacctgtcca ccggcatcaa
     1081 cccgcagggc tggccggtgc cgccgctgcc ggcggcagcc tggcagcgcc tgccggaaga
     1141 caacgacggc ctggaggccg ccgctgccgc ctactacggc aacgccaacc tgctgccggt
     1201 ggccggttcg caggccgcca tccagtggct gccggcgctg ttaccgcgcg ccgcggtggc
     1261 ctgcatcgcg ccgctctaca acgaacatcc gcaggcctgg cagcgcgccg gccacaagct
     1321 gcgcttcctg cagaacgcga cgctgccgcg cgcgttggcg gcggcaaccc cttacgtgct
     1381 gctgtgcaac ccgaacaacc cgaccgccga ccgccatccg cgcgagctgg cggtcgacgc
     1441 cgcggcgcag ctgaaaaagc gcggcggctg gctgatcgtc gacgaggcgt tcgtcgacgc
     1501 cacacccgag catagcctga cgccgctggc cggcacggcg gacgccccta acctgatcgt
     1561 cttccgttcg ctgggcaagt tcttcggcct ggccggactg cgcgtcggct tcctgttcgc
     1621 cgcccccgag ctgctggcga agatggccga agcgatcggc ccgtggagca tcgccggccc
     1681 gtcacgggcg gcggcaacgt tggcgctggc cgaccgcgcc tggcaggcgg caacgcgcga
     1741 gcgcctgcag gccgacggcg aacgcctgcg ccagttgctg gcgccgtacg gcgagctcaa
     1801 ggcaacgccg ctgttcgcga cactgaacgc gccggctacc gccgaactgc acgaacatct
     1861 ggcgcgccgc gccatcctca gccgccgtta cgaatcgaac ccgctactac gcgtcggcct
     1921 gccgcccgac gaagccggct ggcagcgcct ggccgacgcg ctcaaggcgt ggaagcccgc
     1981 atgaaacgcc gtctctttgc cctgcttgcc gccgcctgcc tgggcggcgc ccacgccgaa
     2041 atcgtcgtgc gcgacgacag cggccgcgac gtccacctgc gcgagccggc acggcgcatc
     2101 gtcgccctcg ccccgcacat tgccgaaacg ctgttcgccg ccggtgccgg cggcaagctg
     2161 gtgggcaccg tcgattacag cgactaccca ccggcggcga agaaagtgcc gcgcgtcggc
     2221 ggctattcgc gcatcgatct ggaagccgtc gtcgccctca agcctgacgt ggtgctggtc
     2281 tgggaaagcg gcaacaacat ggcgcaggcc gacaagctgc gggcgctcgg cctgaccgtt
     2341 ttcgtttcgc agcccaaccg catggacgat atcgccggcc aggtcgaacg ttacggccga
     2401 ctggccggca ccgaggccat cgccaacgcg gaggcccacc gcttccgcga gcgtctggcg
     2461 gcgctgcgcg cggccaacgc cggcaaaccc aaggtgcgga cgttctacca agtatggaag
     2521 gcgccgctgg tcaccgtcgg cggtccgcag atcatcagcg acgcgatgcg catctgcggc
     2581 ggcgaaaacg tgttcggcca gctcgaccag atggcgccgc gggtcagcct ggaagccgtg
     2641 atcgaagccg atccggaagc catcgtcgcc accggcatgg gcgacgccaa accggaatgg
     2701 ctggacgact ggcggcagtg gaagcggatg accgccgtcc agcgcggcaa cctcttccac
     2761 atcaacccgg acatcatgca gcggcacacg ccgcgcattc tcgacggtac ggcgaaactc
     2821 tgcgaccacc tcgatgtcgc acgcagccgt cgccccaagt gagaacatca tgtcccgcct
     2881 cccccaacgc atcgtctgcc tgaccaccga aaccgtcgaa gtcctctacg ccctgggcga
     2941 acaggagcgc atcgtcggca tcagcggcta caccgtgcgg ccgccgcagg cacgcaagga
     3001 aaagcccaag gttttcgcct tcaccagcgg cgacatcgcc aagatcctcg ccgtcgaacc
     3061 cgacctggtg ctgaccttct ccgacctgca ggccgacatc gcgcgcgacc tgatcaaggc
     3121 cggcattccg gtctacgcac tgaacatgcg cagcgtcgac gacatcctgg cgatgatcga
     3181 aacgctcggc cgcctggtcg gtgccgagga tcgcgcgctg gcgctggtcg ccgatctgga
     3241 ggcgcagatt gccgcagccc gggcacatgc cgaggccagc atcgcccgcc tcggccgccg
     3301 gccgcgcgtc tatttcgagg aatgggacga accgctgatc agcggcctgc gctgggtttc
     3361 cgaactgatc gaaatcgccg gcggcgagga cgtcttcgcc gaacgcgccc gctcgccgct
     3421 cgccgccgac cggcggccga cgccggaaga agtgatcgcc gccgccccgg acatcatcat
     3481 cggctcctgg tgcggcaagc acttccgccc cgaacgcgtc gccgcccgcc cgggctggga
     3541 agccatcccg gcggtcaagc aggggcgcct gcacgagatc aagtcggcaa tcgtcctgac
     3601 cccgggaccg gtggcgatca gcgaaggcct gccactgctg gaaaagatcc tctggccggc
     3661 aagcgcctga cggtaaacgc ttattgccat ggcatcacgc aaacccctat agttattgca
     3721 tgttgcgttc ggcattgatt ctcctgattt tttccctgtt cacgctgctt gcgcgggcag
     3781 gggagatcgc catcgtcctg gccgacagca gcgcccccta tcaggaattc gcgagcggcc
     3841 tgcaacagcg gctcaaaggc agcaattggc agatcagtta tgtcgatacc gaaccacatg
     3901 ctccggagcg ccaaccggtc gcactgatcg tcaccgccgg cagcgaggct ttgcgcaaga
     3961 cgctggcgag ttcgccgaag gtgcccgtcc tcgccaccct gctgaccgag cacgcctatc
     4021 agaatcaaat cggcaccagc cgcccgccgg cggtctcggc gatctatctc gatcaaccgg
     4081 cgcagcgcca ggctcgcctg ctgcgcctgc tctttcccga gctgcagcgg gtcggtttgc
     4141 tgctcagcga gcagtcgcgc caacaggccg acagcctgcg caccgccctc gccggccagc
     4201 gcctgcaacc ggtcagcgag ttcgccaacg gcgaggagta catcctgccg acactggaag
     4261 cgctgatgca gcgcagcgaa gtactgctcg ccctgcccga tcctctgctc tattcgcgca
     4321 acacgatcaa acccatcctg atcacctcgt accgccaccg gcgaccggtg atcgccttct
     4381 cgcccgcgat ggtcaaggct ggcgcgcttg cggcgctgta ctcgacgcca ctgcagattg
     4441 gccgccaggc cgccgaactg ctcatcgcac atggtaccca cctgccgtta ccggccaccc
     4501 catcaaaatt ctcgatcgac atcaaccgca gcgtcgccga cgccttcggc ctgcgcctgc
     4561 cggaggagag cgatctgttc cagaaactgc ttgccagtga agtcaatcca tgaagaacct
     4621 gaccctgcgc caacgcctgc tggttctgac gctgctgccc agcgccctga tcaccacgct
     4681 gctcgtcgtc tatttcgcca tcaccggcat caacaccctg gaaaaccaac tacgcgccaa
     4741 agggttggcc accgtccgct acctggcgcc gatcagtgaa tacggcatca ttgccggtca
     4801 gatggacagc gtttacggtc tggtccaggc cgccatgcag gaacccggga tcaaagcggc
     4861 gatcatcgtc aatccgaagg gacgcaccct ggccgtcagc gggcgcgtct cactttcggc
     4921 cgaaaccatt cgtcaacgct tgaacgcccc cgccctggtc ggcgagggcg acacctggat
     4981 tgccttcggt gcgccggtcg tgcgcagcca gagcgacagc gacccgctgt tcgacatcgc
     5041 cggcctcgac gagaaccccc aggaaaccat cggtcacgtc ttcattgaac tggacaagga
     5101 agagctgcag gtccgccagc aggaactggt gctgcaaggt ttcatcgccg tactctgcgg
     5161 cctcggcctg atgggcgccc tcgccatcgc catggccgac cacctggcgc gcccgctgca
     5221 acgactcgcc gccgccgtcc gcaacatgtc ggctggacgc ttcgacaccc gcgtccaggc
     5281 cagttcgagc ggcgagctgc gggtactcga agatggcttc aacgagatgg ccagccatat
     5341 cgaggaagcc catcagtcga tgcagacacg gatcgaggaa gccaccgccc aacttgtctt
     5401 ccaggcccgc cacgacgcgc tgaccgggct ggtcaaccgg cgcgaattcg aactgcgcct
     5461 cgacaaggcg ctggccgccg tccatgccgg cggcgacgaa ttcagcgtgc tctacatcga
     5521 cctcgaccgc ttcaaggcgg tcaacgatgc ctgcggccat atcgccggcg acgaactgct
     5581 gcgccagatc gccctcctct tccagggacg cctgcgcgag gaagacacgc tggcccgcct
     5641 gggcggcgac gaattcggca tcatgctggc caactgcagc ctggccaagg ctcgccaggt
     5701 ggcgcaggac atctgcgcgc tctcggccga ataccgcttc gtctggcagg acaagatctt
     5761 cgccatcggc gccagcatcg gtctgacgcc ggtcacccgc gacgtgcgcg acgttcatga
//
LOCUS       DAOSDX010000049         5774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_4967_length_5774_cov_2.616017, whole genome shotgun sequence.
ACCESSION   DAOSDX010000049 DAOSDX010000000
VERSION     DAOSDX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4967_length_5774_cov_2.616017"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLN02_01795"
     CDS             complement(<1..306)
                     /locus_tag="PLN02_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51082.1"
                     /translation="MRPITLRYRPEKRRLRWLLTLPKLGLVLLMAALLTLLWLLQRSE
                     HEEQRNILIKDVLWLEQNLRFHLEGNRDLMQQLAVDMADPREREKLFQVRSTHLLKNS
                     "
     gene            436..1440
                     /locus_tag="PLN02_01800"
     CDS             436..1440
                     /locus_tag="PLN02_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOI51083.1"
                     /translation="MKVSKLLVGLFACAVSLGAYAQQPIVIKFSHVVANDTPKGKAAD
                     FFAQKAAELTKGKVKVEVYANSTLYKDKEEMEALQLGAVQMLAPSLAKFGPLGVKEFE
                     VFDLPYIFNGYDDLHKVTTGPVGKQLLAKLEPKGISGLAFWDNGFKSFSANTPIKMPE
                     DLKGKKLRIQSSKVLEEQIRALKALPQVMAFSEVYQALQTGVVDGTENPISNLYTQKM
                     HEVQKHLTLTEHGYLGYAVIVNKKFWDGLPADIRKQLDEAMVQATRYANQIAKVENDT
                     ALENVRKSGKTQVYSPTAAERSAFKKALVPVHKKMESRIGKETIGAVYKATGFDPNKL
                     "
     gene            1510..2226
                     /locus_tag="PLN02_01805"
     CDS             1510..2226
                     /locus_tag="PLN02_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOI51084.1"
                     /translation="MGKIIDRLEEFIIGTLMAVATVVIFVSVVHRYGTGFEIPGVQDW
                     LLEINMGWAQEFCIILFVWMAKFGAAYGVRTGIHVGVDIVINKLPSKPRAVMVQLGLF
                     CGVLFTAVIGVFGAAFVWENGMAYETLSLLGSDTGAHFEGPTTPDLEWPTWMVYSAVP
                     LGSFLMCFRFIQVMIKFARSGELPTHDHGHVEGLEEGEVLVVGEAIAIAEQREHAHAG
                     LSDADWEKEHGAEDAANKEK"
     gene            2230..3513
                     /locus_tag="PLN02_01810"
     CDS             2230..3513
                     /locus_tag="PLN02_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOI51085.1"
                     /translation="MSALIIFGLLLTLMVIGMPVGVALGLTVLGFMFAFTETPIASVA
                     LKMFTGIEKFEIMAIPFFILAGNFLTHGGVARRMINFATSMVGHLTGGLGMSAVLACA
                     LFAAVSGSSPATVVAIGSILIPAMLKQGFPMNFSAGVVASAGGLGILIPPSIVMVMYA
                     VTTNSSVGALFMAGVVPGLLLALTLGVTTWWQARKGAYPTLPKASWGERLRTFREAFW
                     GLMLIVVVMGGIYSGMFTPTEAAAMSAVYAFFIAVFVYKDLTFKQIPKVLLDSANMSS
                     MLLFIIASAILFSFILTSEQIPQDIADWIVHLGLGPVGFLVIVNILLLVAGNFMEPSS
                     IILILAPILFPVAVALGIDQVHFGIIITVNMEIGMITPPVGLNLFVASGITRRGLSEM
                     SVAVLPWLYAMLVFLIAITYIPEISLWLPRTLGML"
     gene            3614..4291
                     /locus_tag="PLN02_01815"
     CDS             3614..4291
                     /locus_tag="PLN02_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51086.1"
                     /translation="MKFALSIIALIVAALIIPFLIPGLGQREGVNPNANLPWQIEVDG
                     QGGSRVFGIRPGTQTLGEVRAILGGEAPEVAIIATPDEVGTLEAYYSELAFGFVLAKV
                     ILTVDTTPDMITAMRDRALKGKYMESSTRKILLHPDDLLAADAMPVRAISIIPKASLD
                     EATVIQRFGAPGARVPIGDKRVHLLYPEKGLDVVVDADGKELLQYVAPRDFERLREPL
                     QKEAAAQ"
     gene            4365..4793
                     /gene="ndk"
                     /locus_tag="PLN02_01820"
     CDS             4365..4793
                     /gene="ndk"
                     /locus_tag="PLN02_01820"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOI51087.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYSRFEGAGLKVIAARMTWLSE
                     QEAGQFYAVHKERPFFKDLVSFMTSGPVMIQCLEGENAIAKNRELMGATDPKKAEKGT
                     IRADFAESIDANAVHGSDAPETAAVEVAFFFPGMNTYAGR"
     gene            4845..>5774
                     /gene="rlmN"
                     /locus_tag="PLN02_01825"
     CDS             4845..>5774
                     /gene="rlmN"
                     /locus_tag="PLN02_01825"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOI51088.1"
                     /translation="MQNLLDLDADGLTAWFAEQGEKPFRARQVLRWMHRSGVADFDAM
                     TDIAKSLRNKLKARAVVAPPTVVSDKLSDDGTRKFLVDVGNGNAVETVFIPEDERGTL
                     CISTQAGCALDCAFCSTGKQGFNRNLTVAEIIGQLWQANRALGAVHGDERVISNVVMM
                     GMGEPLANYDNTVAALRLMLDDNAYGLSRRRVTVSTSGLVPAIDRLREECPVALAVSL
                     HAPNDALRDQLVPINQKYPLRELMAACRRYLEDAPRDFITFEYVLLAGVNDGDADARA
                     LLALTRDIPCKFNLIPFNPFPGSDYRKPGADRVR"
BASE COUNT         1042 a         1799 c         1778 g         1155 t
ORIGIN      
        1 gctattcttg agcaagtggg tcgagcggac ctgaaagagt ttttcccgtt cccgcggatc
       61 ggccatgtcg acggccagtt gttgcatcag gtcgcgattg ccttcgaggt ggaagcgcag
      121 gttctgttcc agccacagca catccttgat caggatgtta cgctgctcct cgtgttccga
      181 gcgctgcagc agccagagca gggtcagcag tgcggccatg agcaggacca ggccaagttt
      241 cggcagggtc agcagccagc gcaggcggcg tttttcgggg cggtagcgga gggtgatcgg
      301 gcgcatggcg gcgatgttaa cccgtggtca acggatgtgg atatccacaa tacgcactcg
      361 ttagcctttt cgtaatactt tgcagcagtc gcaatcggat ttcgcgacat tccaacttca
      421 ggaggagata tatcaatgaa agtttccaag cttctcgtcg gcctgtttgc ctgtgccgtt
      481 tcgctcggtg cctatgcgca gcagccgatc gtcatcaagt tcagccacgt cgtcgccaat
      541 gacacgccca agggcaaggc cgccgatttc ttcgcccaga aggctgccga actgaccaag
      601 ggcaaggtca aggtcgaggt ttatgccaac tcgacgctgt acaaggacaa ggaagagatg
      661 gaagcgctgc agcttggcgc cgtgcagatg ctcgccccgt cgctggccaa gttcggcccg
      721 ctcggcgtca aggaattcga ggtcttcgac ctgccctaca tcttcaatgg ctacgacgac
      781 ctgcacaagg tcaccaccgg cccggtcggc aagcaactgc tggccaagct cgaacccaag
      841 gggatcagcg gcctggcttt ctgggacaac ggtttcaaat ccttctcggc gaataccccg
      901 atcaagatgc cggaagacct gaagggcaag aagctgcgca tccagtcgtc caaggtgctc
      961 gaagagcaga ttcgcgcgct gaaggcactg ccgcaggtga tggccttctc cgaggtctat
     1021 caggcgttgc agactggcgt tgtggacggt accgagaacc cgatctccaa cctctacacc
     1081 cagaagatgc acgaagtgca gaagcacctg acgctgaccg agcacggcta cctgggctac
     1141 gcggtgatcg tcaacaagaa gttctgggac ggtctgccgg ccgacatccg caagcagctc
     1201 gacgaggcca tggtgcaggc gacgcgttac gccaaccaga tcgccaaggt ggaaaacgac
     1261 acggcgctgg aaaatgttcg caagagcggc aagacccagg tctattcgcc gacggcggcc
     1321 gagcgttcgg ccttcaagaa ggccctggtg ccggtgcaca agaagatgga aagccggatc
     1381 ggcaaggaaa ccatcggcgc ggtctacaag gccaccggtt tcgatccgaa caagctgtaa
     1441 gcaacaattg acctccgacg cccgctgttg cgctttgccg cagcgggcgt ttttgcaagg
     1501 agaggggaca tgggaaaaat cattgaccgg ctggaagagt tcatcatcgg caccctgatg
     1561 gcggtggcga ccgtggtcat cttcgtttcc gtggtgcatc gctacggaac gggtttcgag
     1621 attccggggg tgcaggactg gctgctcgaa atcaacatgg gctgggccca ggaattctgc
     1681 atcattcttt tcgtctggat ggccaagttc ggcgccgctt acggcgtgcg caccggcatc
     1741 cacgtcggcg tcgatatcgt gatcaacaag ctgccgagca agccgcgcgc cgtcatggtg
     1801 cagctcggct tgttctgcgg ggtgctgttt actgcggtga tcggcgtttt cggcgccgct
     1861 ttcgtctggg aaaacggcat ggcttacgag acgctgagcc tgcttggcag cgacaccggc
     1921 gctcacttcg aggggccgac cacgcctgac ctcgaatggc cgacctggat ggtgtactcg
     1981 gcggtaccgc tgggctcctt cctgatgtgc ttccgtttca tccaggtgat gatcaagttt
     2041 gcccgcagcg gcgagttgcc gacccatgac cacggtcacg tcgaaggcct ggaagagggc
     2101 gaagtgctgg tcgtcggcga ggcgatcgcc attgccgaac agcgcgaaca cgctcatgcc
     2161 ggcctttctg atgccgactg ggaaaaagag catggcgcgg aagacgcggc caacaaggag
     2221 aaataagcga tgagcgctct gattatcttt ggcctgctgc tgaccctgat ggtcatcggc
     2281 atgccggtcg gcgtcgccct cggcctgacc gtcctcggct tcatgttcgc cttcaccgag
     2341 acgccgatcg cttcggtggc gctgaagatg ttcaccggca tcgagaagtt cgagatcatg
     2401 gcgatcccct tcttcatcct cgccggcaac ttcctcaccc acggcggtgt cgcccggcgc
     2461 atgatcaact tcgccacttc gatggtcggt cacctgaccg gcggtctcgg catgtcggca
     2521 gtgctggcct gcgcgctgtt cgcggcggtg tccggttcgt cgccggcaac cgtggtggcg
     2581 atcggctcga tcctgattcc ggccatgctc aagcagggct ttccgatgaa cttctcggcc
     2641 ggtgtcgtcg cttcggccgg cggtctgggg atcctgattc cgccgtcgat cgtcatggtg
     2701 atgtacgcgg tgacgaccaa ttcgtcggtc ggcgccttgt tcatggccgg cgtcgttccc
     2761 ggcctgctgc tggcgctgac gctcggtgtc accacctggt ggcaggcgcg caagggcgct
     2821 tacccgaccc tgcccaaggc ttcctggggc gagcgtctgc gcaccttccg cgaagctttc
     2881 tggggcttga tgctgatcgt cgtggtcatg ggcggcatct actccggcat gttcacgccg
     2941 accgaagcgg cggcgatgtc ggcggtctat gccttcttca tcgcggtgtt cgtgtacaag
     3001 gacctgacct tcaagcagat tccgaaggtg ctgctcgact cggccaacat gagctcgatg
     3061 ctgctgttca ttatcgccag cgccatcctg ttcagcttca tcctgacctc ggagcagatt
     3121 ccgcaggata tcgccgactg gatcgtccat ctcggtctgg ggccggtcgg tttcctggtc
     3181 atcgtcaaca tcctgctgct ggttgccggc aacttcatgg agccgtcgtc gatcatcctg
     3241 atcctggcgc cgatcctgtt cccggtggcc gtcgcgctgg gcatcgatca ggtgcacttc
     3301 ggcatcatca tcacggtgaa catggaaatc ggcatgatca cgccgccggt cggtctcaac
     3361 ctgttcgtcg ccagcggcat cacccgccgc ggcctgtccg agatgtcggt ggcagtgctg
     3421 ccgtggctgt acgcgatgct ggtcttcctg atcgccatca cctacattcc ggaaatctcg
     3481 ctctggctgc cgcgtaccct gggcatgctc tgagtcgttc gggtcaagcc gacgcaggcc
     3541 gcgctttccg caaggggcgc ggccttttcc ttctctgtgg cacaattcgg cttccgccgc
     3601 ccacctgtcc atcatgaaat tcgccctctc catcattgcg ctgatcgttg ccgcgctgat
     3661 cattcctttc ctcattcccg gtctcggtca gcgggaaggg gtcaatccga atgccaacct
     3721 gccctggcag atcgaagttg acggccaggg gggcagccgt gtcttcggta tccggccggg
     3781 cacgcagacg ctgggtgaag tgcgtgcgat cctcggcggc gaggcgccgg aagtggcgat
     3841 catcgccacc ccggacgaag tcggcacgct cgaagcctac tattcggaat tggcgttcgg
     3901 cttcgtgctg gccaaggtca tcctgactgt cgatacgacg ccggacatga tcacggcgat
     3961 gcgcgacagg gcgctcaagg ggaagtacat ggaaagctcg acacgcaaga ttctcctgca
     4021 tccggacgac ctgcttgccg ccgacgccat gccggtacgg gcgatcagca tcattcccaa
     4081 ggccagtctc gacgaggcga ccgtcatcca gcgtttcggt gcaccggggg cgcgtgtgcc
     4141 gatcggcgac aagcgtgttc acctgctcta cccggaaaaa gggcttgatg tcgtggtcga
     4201 cgccgacggc aaggaattgc tgcaatacgt tgcgccgcgc gatttcgagc gcctgcgcga
     4261 acccctgcaa aaggaagccg ccgcccagta aggcgggtgt ctcccgtcct tatctgctaa
     4321 aattccccgt ttttcaaacg actagctttt ttcggagcaa acacatggct atcgaacgca
     4381 ctctttccat catcaagccg gacgccgttg ccaagaacgt catcggtcag atctactccc
     4441 gtttcgaagg cgccggcctc aaggtcatcg ccgcccgcat gacctggctg tccgagcagg
     4501 aagccggcca gttctacgcc gtgcacaagg aacgtccttt cttcaaggac ctggtctcgt
     4561 tcatgacctc cggcccggtg atgatccagt gcctggaagg cgagaacgcc atcgccaaga
     4621 accgcgagct gatgggcgcc accgacccga agaaggccga gaagggcacc atccgcgccg
     4681 atttcgccga gtcgatcgac gccaatgccg tgcatggttc cgatgcgccg gaaactgccg
     4741 ctgtggaagt tgccttcttc ttcccgggca tgaacaccta cgctggtcgc tgatttgttg
     4801 ttgtcgtgcc ggccgttggg gctggcacga accccgggat gcagatgcag aatctccttg
     4861 atttagatgc cgacggcctg accgcctggt ttgccgagca gggcgaaaag cccttccggg
     4921 cgcgccaggt gctgcgctgg atgcatcgtt ccggggtggc ggatttcgat gccatgaccg
     4981 acatcgccaa gagcttgcgc aacaagctca aggcgcgggc ggtcgtggcg ccgccgacgg
     5041 tggtctccga caagctgtcg gacgacggga cgcgcaagtt cctggtcgac gtcggcaacg
     5101 gcaacgcggt cgagacggtg ttcattcccg aggacgagcg cggcaccctg tgcatttcca
     5161 cgcaagccgg ttgcgcgctc gattgcgcct tctgttcgac cggcaaacaa ggtttcaacc
     5221 gcaacctgac ggtggcggaa atcatcggcc agctctggca ggccaaccgg gcgctgggcg
     5281 ccgtacacgg cgacgaacgg gtgatctcca acgtcgtcat gatgggcatg ggcgagccgc
     5341 tcgccaacta cgacaatacc gtcgccgccc tgcgcctgat gctcgacgac aacgcctacg
     5401 ggctgtcgcg gcgccgggtc acggtgtcga cttccggcct ggtgccggcg atcgaccgcc
     5461 tgcgcgagga atgcccggtc gccctggcgg tctcgctgca cgcgccgaac gatgcgctgc
     5521 gcgaccagct ggtgccgatc aaccagaaat atcccctgcg cgaactgatg gccgcctgcc
     5581 ggcgctatct ggaagacgcg ccgcgcgatt tcatcacctt cgaatacgtg ctgctcgccg
     5641 gcgtcaatga cggcgatgcc gacgcccggg cgctgctcgc gctgacgcgc gacatcccct
     5701 gcaagttcaa cctgattccg ttcaacccct tccccggttc cgactatcgc aagccgggcg
     5761 ccgaccgcgt gcgc
//
LOCUS       DAOSDX010000050         5716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5032_length_5716_cov_3.982689, whole genome shotgun sequence.
ACCESSION   DAOSDX010000050 DAOSDX010000000
VERSION     DAOSDX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5032_length_5716_cov_3.982689"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..146
                     /locus_tag="PLN02_01830"
                     /pseudo
     CDS             1..146
                     /locus_tag="PLN02_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764237.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            168..1103
                     /gene="hprK"
                     /locus_tag="PLN02_01835"
     CDS             168..1103
                     /gene="hprK"
                     /locus_tag="PLN02_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOI51089.1"
                     /translation="MRHISLEQLYQDNREQLLFNWVVGQRADRRVELKAASNYGADVV
                     GHINIIHPERLQVMGQAEYDWAMRIGERRFGQLFSDLLSARTPAMIVADGLKPPDFVL
                     EACRLADVPLILSPKHCSAVIDHLRIYLSARLADTVSLHGVFMDVLGMGVLITGDSGV
                     GKSELALELISRGHGLVADDVVEMARIAPTTIEGRCPGMLRDFLEVRGLGLLNIRTIF
                     GETASRRKMKLKLIVHLQRTIAAEDAPRLPLDAQTQEVLGVPVRKVVIPVAAGRNLAV
                     LLEAAVRNTILQYRGIDSMQDFIDRQSRVMNDETL"
     gene            complement(1125..2024)
                     /locus_tag="PLN02_01840"
     CDS             complement(1125..2024)
                     /locus_tag="PLN02_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOI51090.1"
                     /translation="MNAQRPIPVIMVADITGSASLHQRLDEQEAERAIDRCIKRMQRA
                     IEAYDGQLLQQVGDEILVSFPTPEDACHAAVDMHQRIADLPPVSGHKLSIRVALHDKG
                     NDANQATQPETLANLMRIGGHARGDQILCSGAIVAALPPSSVITTQVRPDVSDLSEAD
                     NKLLVFEINWPAKTVATPQHHSMFGPQNAPRIAERLCLRYRGQAYLIDSKAPVISLGR
                     DPSCKLLVENRRVSRLHARIERRDDGYYLVDTSTNGSFLTLQGRREIMVRKHEALLDG
                     RGIICFGSSANDTAAERIEFEHL"
     gene            complement(2080..2949)
                     /gene="ttcA"
                     /locus_tag="PLN02_01845"
     CDS             complement(2080..2949)
                     /gene="ttcA"
                     /locus_tag="PLN02_01845"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOI51091.1"
                     /translation="MDTPVSANLQKLRKFLEGRTGKAIMDYNMIADGDTVLVCCSGGK
                     DSYTLLAMLMALQKRAPVKFRLIAMNLDQKQPGFPADVLPAYFDSIGIEHRIVEADTY
                     SIVKDKIPEGKTTCSLCSRLRRGIIYRTAKELGANKIALGHHRDDMVHTLFLNMLFGG
                     KMKAMPPKLVTDDKAHVVIRPLAYCSESDIAKFARGMAFPIIPCNLCGSQENLQRQKI
                     KEMMQDWDRRYPGRTESVFTAMQNVVPSHLADNTLFDFSGLTLDTQVDEGDIAFDTEP
                     LEFSGAIPISPLA"
     gene            3057..4217
                     /locus_tag="PLN02_01850"
     CDS             3057..4217
                     /locus_tag="PLN02_01850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI51092.1"
                     /translation="MSQLPAPSPEALAHSARLQAEICRAIDQQGGWLSFADFMEMLLY
                     APGLGYYTAGARKFGSAGDFVTAPEMTSLFGEALARQLAQVMALSVPQVLEVGAGSGR
                     LAADLLLALEGLGQLPERYAILDLSADLRQRQQETLARAAPHLLARVEWLDRLPDEFS
                     GAVLANELLDALPAHLVRWNGDEIRECGVVRDADGGFMWNERAAGGALLAAAREIADS
                     CELEPGYLSEISLAARAWAAEWGHRLGCGALLLIDYGFPRHEYYHPQRGAGTLMCHYR
                     HHAHADPFYLPGLQDVTVHVDFTAIIAAAHAAGLDLLGYASQGQFLLNCGMLDLLAAI
                     PRDTPDYVRAASAAGKLLMPHEMGELFKVMAIGRGIDGGLLGFAQGDQGRRL"
     gene            4269..4460
                     /locus_tag="PLN02_01855"
     CDS             4269..4460
                     /locus_tag="PLN02_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HOI51093.1"
                     /translation="MDTSGIGSFNTAMSAEQTSMAVSTAVLKKAIDIEEQNSMQLLQT
                     LPPTYNNPANLGTQVDVKA"
     gene            complement(4450..5256)
                     /locus_tag="PLN02_01860"
     CDS             complement(4450..5256)
                     /locus_tag="PLN02_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI51094.1"
                     /translation="MIKKQNIAGLEVFSCLPDTPTSRPNLLFIHGAFAGAWMWTETYM
                     PYFAAAGYPCHALSLRGHGDSDGHDRINWLSVADYVDDVRMVIEALAIDPVLLGHSMG
                     GFVVQKYLEHHAAPAVALLCSVPPQGLIAAQFHLLLQKPQLYLEINRILAGNYTETDT
                     LKEALFAGEVDEKMLETWLGRMQNESQRAIWDMSMFNLPNLYCMQKAPMLVVGAEMDA
                     LVPAFLAQSTAQTYGEPCHILRGMGHAITHEKDWTKGAALILNWLETLKP"
     gene            5315..5512
                     /locus_tag="PLN02_01865"
     CDS             5315..5512
                     /locus_tag="PLN02_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOI51095.1"
                     /translation="MLKWLLTLVVAIFLLGLVTPHLARFIRFGQLPGDIALRWRGRVY
                     HFPFVSILLFSLLLWLIGRLL"
     gene            complement(5477..>5716)
                     /gene="cca"
                     /locus_tag="PLN02_01870"
     CDS             complement(5477..>5716)
                     /gene="cca"
                     /locus_tag="PLN02_01870"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HOI51096.1"
                     /translation="DQLLDACLCDYTGRGGWQDRPYTAPARLHKALAAVSALDAGKIA
                     AASPNPGSIPERIRQARIAAVRQTLEEAPDQPEQQ"
BASE COUNT          989 a         1680 c         1861 g         1186 t
ORIGIN      
        1 ccctgctttt cgtcctgctg gtaccggaac aggcgaccga ggaacatctg cagatcctgt
       61 ccgagctggc gcaacgcttc tccgatcgcg ctttccgcga agccctgctg gcagctcccg
      121 acgtagcgac cgttctctcc ctgttcaaga aatcctgatt ccgcgccgtg cgccacatca
      181 gccttgagca gctatatcag gacaaccgcg agcagttgct gttcaactgg gtggtcggtc
      241 agcgcgccga ccgccgggtc gaactgaagg cggccagcaa ttacggcgcc gacgtcgtcg
      301 ggcacatcaa catcatccac ccggaacgcc tgcaggtcat ggggcaggcc gagtacgact
      361 gggcgatgcg catcggcgaa cgccgtttcg gccagttgtt cagtgacctg ctgtcggcca
      421 ggacgccggc gatgatcgtc gccgacggac tcaagccgcc tgatttcgtg ctcgaagcct
      481 gtcgcctggc cgatgtgccg ttgatcctct cgcccaagca ctgttcggct gtcatcgatc
      541 acctgcgcat ctatctttcc gcccgcctcg ccgacacggt cagcctgcac ggtgtgttca
      601 tggacgtgct cggcatgggc gtgctgatca ccggcgattc cggggtcggc aagtcggaac
      661 tggcgctcga actgatttcc cgcggccatg gcctggtggc cgacgatgtc gtggaaatgg
      721 cgcgcattgc accgaccacg atcgagggcc gctgcccggg catgttgcgc gattttctcg
      781 aggtacgtgg gcttgggctg ctcaacatcc ggaccatctt cggtgaaacg gcgtcgcgcc
      841 ggaaaatgaa gctgaagctg atcgtccatc tgcagcggac gatcgctgcc gaagatgcgc
      901 cacgcttgcc gctcgatgcc cagactcagg aagtgctggg cgttccggtg cgcaaggtgg
      961 tgattcccgt cgccgccggg cgcaacctgg cggtacttct ggaagcggcg gtacgcaaca
     1021 ccatcctgca gtatcgcggg atcgattcga tgcaggattt cattgaccgg cagagccggg
     1081 tgatgaacga cgaaacgctt tgaaaagcgg cgttgccgcg ctgcttacag gtgttcgaat
     1141 tcgatgcgct cggcggccgt atcgttggcc gaactgccga agcagatgat gcctctgccg
     1201 tcgagcaggg cctcgtgctt acgcaccatg atttcccgcc tgccctgcag ggtcaggaag
     1261 ctgccgttgg tgctggtgtc gaccagataa tacccgtcgt cacggcgctc gattcgggca
     1321 tgcaggcggg agacgcgacg gttctccacc agcagcttgc atgaggggtc gcgtcccagg
     1381 ctgatcaccg gcgctttcga atcgatcagg taagcctgtc cccggtaacg cagacagagt
     1441 cgttcggcga tccgcggtgc attttgcggc ccgaacatcg aatgatgctg cggcgtggcg
     1501 acggtttttg ccggccagtt gatctcgaag accagtaact tgttgtccgc ttcggagagg
     1561 tcgctgacat ccgggcgcac ttgcgtggtg atgacgctgc tcggcggcag cgcagcaaca
     1621 atcgcgccgc tgcacagaat ttgatcgccc cgggcgtgcc cgccgatacg catcaggttg
     1681 gcgagagttt cgggttgtgt cgcctgattc gcatcgttgc ccttgtcgtg cagggcaacg
     1741 cgaatgctca gtttgtgacc ggaaaccggg ggcagatcgg cgatccgctg atgcatgtcg
     1801 accgccgcgt ggcaggcgtc ttccggagtt ggaaaggaaa ccaggatttc atcgcccacc
     1861 tgctgcagca actggccatc gtaagcttcg atggcacgct gcatgcgctt gatgcagcgg
     1921 tcgatggcac gttccgcttc ctgttcgtcc agtcgctgat ggagcgaagc gctgccggtg
     1981 atatcggcga ccatgatgac ggggatcggg cgctgcgcgt tcatagctgg actggatgga
     2041 aaggacgtcc gattatatga cggactgcat ttcgtggcat caggcgagcg gggaaattgg
     2101 aatcgcgccg ctgaattcca gcggttcggt gtcgaaggcg atatcgccct cgtcgacctg
     2161 cgtatccagc gtcagcccgc tgaaatcgaa cagcgtgttg tcggcaagat gcgacggcac
     2221 gacgttctgc atggcggtga acacggattc ggtccgtcct gggtaacggc ggtcccaatc
     2281 ctgcatcatt tccttgatct tctgccgttg caggttctcc tgcgagccgc agaggttgca
     2341 gggaatgatg ggaaaagcca tgccgcgggc gaacttggca atatcgctct cactgcagta
     2401 agccagcggc cggatcacga catgggcctt gtcgtcggtg accagtttcg gcggcatcgc
     2461 cttcatcttg ccgccaaaca gcatgttcag gaacagggtg tggaccatgt cgtcacggtg
     2521 gtgacctaac gcgatcttgt tggcgccgag ttccttggcg gtgcggtaga tgatgccgcg
     2581 acgcagtcgc gagcacagcg agcaggtggt ctttccctcg gggatcttgt ccttgacgat
     2641 cgaataggta tcggcctcga cgatgcggtg ctcgataccg atcgaatcga agtaagccgg
     2701 caggacatcg gccggaaatc cgggctgctt ctggtcgagg ttcatggcga tcaggcggaa
     2761 tttaaccggc gcccgcttct gcagcgccat cagcatggcg agcagggtgt aggaatcctt
     2821 gccgccggag caacagacca ggacggtatc cccgtcggcg atcatgttgt agtccatgat
     2881 cgccttgcca gtgcgacctt cgaggaattt gcggagtttt tgcaggttgg cggagacggg
     2941 agtatccatg atcggcggtc tttgctcgtt ggagtggcgg tcgttgcggc cgcaagttta
     3001 cacggatcgc ccgtgaacgc gtcgccggcc gttagaatgc cgctatttgc ccgcccatga
     3061 gccagcttcc cgcgccctct ccggaggcgc tcgcacacag cgcccgtctg caagccgaaa
     3121 tctgccgcgc catcgatcag caaggtggct ggctgtcgtt cgcggatttc atggaaatgc
     3181 tgctctatgc gccggggctc ggctattaca cggcgggcgc gcgcaagttt ggcagtgccg
     3241 gcgacttcgt caccgcgccg gagatgactt ctttgttcgg tgaggcgctg gcgcggcagc
     3301 ttgcacaagt catggcgctt tccgtgccgc aggtgcttga agtcggcgcc ggttccgggc
     3361 ggttggcggc cgatcttctg ttggcgctgg agggcctcgg gcaattgccg gagcgctacg
     3421 cgatcctcga tctctccgcc gatctgcgcc agcgccagca ggagacgctg gcccgggcgg
     3481 cgccacatct gctggcccgg gtcgaatggc tggatcgcct gcccgacgaa ttcagcggcg
     3541 ccgtgctggc caacgaattg ctcgatgcct tgccggctca tctggtgcgc tggaatggcg
     3601 acgaaatccg cgaatgcggc gtggtccgtg atgccgacgg cggtttcatg tggaacgaac
     3661 gtgctgccgg cggagccttg ctcgccgctg cgcgcgagat tgccgacagt tgcgaactgg
     3721 aacccggcta cctgagcgaa atcagcctcg ccgcgcgtgc ctgggcagcg gaatggggac
     3781 accgactggg ttgcggcgcc ctgctcctga tcgactatgg atttccccgc cacgagtact
     3841 accacccgca acgcggggcc ggcaccctga tgtgtcacta ccggcaccac gcccatgccg
     3901 atcccttcta cctgcctggt ttgcaggatg tgacggtgca tgtcgatttc acggcgatca
     3961 tcgccgccgc ccatgctgcc ggactcgatc tgctgggtta cgcgagccag ggacaattcc
     4021 tcctcaactg cggcatgctc gacctgctgg cggcaattcc ccgtgatacg ccggactacg
     4081 tccgggccgc ctccgccgcc ggaaaattgc tgatgccgca cgaaatgggc gaattgttca
     4141 aggtgatggc gattggtcgc ggtatcgacg gcggactgct ggggtttgcc cagggcgacc
     4201 agggtcgccg tttgtaagaa agtggtctaa actggaagcg cttaacagaa gctagcggga
     4261 gcagaatcat ggacacttcg ggtatcggca gtttcaatac ggcgatgagc gctgagcaga
     4321 ccagcatggc cgtgtccacg gccgttctga agaaggcgat cgatatcgag gaacagaact
     4381 cgatgcagtt gctgcagact ttgccgccga cctacaacaa cccagcaaat ctcggcactc
     4441 aggtcgacgt caaggcttga gcgtttccag ccagttcagg ataagcgccg caccttttgt
     4501 ccagtctttc tcgtgcgtga tcgcgtgacc catgccgcgc aggatgtggc atggttcgcc
     4561 gtaggtctgg gcggtggatt gtgcgaggaa ggccggcacg agggcgtcca tttcggcgcc
     4621 aacaacgagc atcggcgctt tctgcatgca gtagaggttg ggcaggttga acatcgacat
     4681 gtcccagatt gcccgctgtg attcgttctg cattcgcccc agccaggttt cgagcatctt
     4741 ttcgtcgact tcgccggcga agagggcttc cttcagcgta tcggtttcgg tgtagttgcc
     4801 ggccaggatg cggttgattt ccaggtagag ttggggcttc tgcagcagca ggtgaaactg
     4861 ggcggcaatc aggccttgcg gtgggaccga gcagagcagc gcaaccgccg gagcggcgtg
     4921 atgttcgaga tatttctgga cgacgaaacc acccatcgaa tggcctagca gcaccggatc
     4981 gatggcgagc gcttcgatca ccatacggac gtcgtcaaca taatcggcca ccgacagcca
     5041 gttgatgcgg tcgtgaccgt cgctgtcacc atggccgcgc agcgacagtg cgtggcaagg
     5101 atagccggcg gccgcgaaat aaggcatgta ggtctcggtc cacatccagg caccggcaaa
     5161 cgcgccgtgg atgaacagca ggttgggccg ggaggtcggc gtgtcgggga ggcaggaaaa
     5221 gacttcgagt cctgcgatat tctgtttttt tatcatcggt ttaccatggg ttcatggggc
     5281 ggccaggttt gtcggtcgcg gggaggtcgg gttcatgctg aagtggttgt tgacgctggt
     5341 ggtcgcgata ttcctgctcg gactggtgac gccgcatctg gcgcgcttca tccgtttcgg
     5401 gcaactgccc ggcgatatcg cgttgcgctg gcggggcagg gtttaccact tcccttttgt
     5461 cagcatcctg ctcttctcat tgctgctctg gctgatcggg cgcctcctct agggtctggc
     5521 ggacggcagc gattctggcc tggcggatgc gctccgggat gctgcccggg ttcggactag
     5581 cggcggcgat ttttccggcg tcgagcgcgc tgaccgcggc gagtgcctta tgcagtctgg
     5641 ctggcgcggt gtacggccgg tcttgccagc cgccacggcc ggtgtagtcg cacaggcagg
     5701 catcgagcaa ctggtc
//
LOCUS       DAOSDX010000051         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5035_length_5714_cov_3.588973, whole genome shotgun sequence.
ACCESSION   DAOSDX010000051 DAOSDX010000000
VERSION     DAOSDX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_5714_cov_3.588973"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLN02_01875"
     CDS             complement(<1..252)
                     /locus_tag="PLN02_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI51097.1"
                     /translation="MPPSSRSTRGLALLLACLSALGPFSIDTYLPSFPDIAQRLGATQ
                     IEVQQTLSAYLFSFAAMTLWHGAIADRFGRRRVILGALAL"
     gene            complement(257..1744)
                     /locus_tag="PLN02_01880"
     CDS             complement(257..1744)
                     /locus_tag="PLN02_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOI51098.1"
                     /translation="MALAVALSRGLDGLDAPPVTVEVHLANGLPGLTLVGLPDTEVRE
                     ARDRVRAAIVNSGFEYPARRITVNLAPADLPKESGRFDLPIALGILAASGQIPAASLN
                     GHEFAGELSLSGELRPVRGALAMALQAAGDGKTFILPAASAGEAALTGQGDILAASSL
                     LAVCAHLAGQTPLTPPAPTTAAPADAPPDLADVRGQAQARRALEVAAAGRHSLLMIGP
                     PGAGKSMLAARLPGLLPALSDGEARASAAVLSLVGQFRAERYRQRIFRSPHHTASAAA
                     LVGGGNPPRPGEISLAHHGVLFLDEIVEFDRKVLETLREPLETGRIHIARAARQAEFP
                     ARFQLVAAMNPCPCGYHGDARCQCTPEQVARYRGKLSGPLLDRIDLIIEVPALPGEAL
                     SGTPSGESSAVVRERVNRAWARQQKRQDSANARLDAVGFDTHCQPDNAGRALLQQAAS
                     RLGLSARAWHRVLRVARTVADLAESDIITAAHIAEAIQYRRFAAD"
     gene            complement(1749..1997)
                     /locus_tag="PLN02_01885"
     CDS             complement(1749..1997)
                     /locus_tag="PLN02_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HOI51099.1"
                     /translation="MLDPKILEDFGNKMSALLANSPAKDIEKNAKALLGGLFGKLDLV
                     TREEFDIQAQLLARTRERLQALEARVEALEKARTDSPQ"
     gene            2239..3084
                     /locus_tag="PLN02_01890"
     CDS             2239..3084
                     /locus_tag="PLN02_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HOI51100.1"
                     /translation="MKKSLIAAALLGAFAAPGAMQVAMADEASPHTITGNLTLASSYR
                     FRGIDQTFGKPTLQGGIDYSHASGFYAGNWNSNVSSGAGFAEGNLEMDFYGGYRTAFG
                     DVGVDVGGIYYYYPGSHSQPLSGTNAKHPTSNASGTVDNTELYVAASYKFLSAKYSYS
                     VGDYFNLPKSKGTSYLELNANYDLGNGWGVNGHVGHLFPSGYKYNTAGSQSISYTDWK
                     VGVTKDVSGWLVGLAYVGTNANGSCSSGEFYCFASSLDKSGALGSKTKDAGRGIAVLS
                     VTKSF"
     gene            3166..3504
                     /gene="glnK"
                     /locus_tag="PLN02_01895"
     CDS             3166..3504
                     /gene="glnK"
                     /locus_tag="PLN02_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOI51101.1"
                     /translation="MKFVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEAAVKADQIDQVIEAIEKSASTGKIGDGKIFVFDLEQVVRI
                     RTGETGGDAL"
     gene            3516..5018
                     /gene="amt"
                     /locus_tag="PLN02_01900"
     CDS             3516..5018
                     /gene="amt"
                     /locus_tag="PLN02_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOI51102.1"
                     /translation="MKRIFAMLALLGAVTFGAPAWAEEKAADAAPVAAAVAEAPAAEA
                     AAPAPAAEAAAPAEAPAAAAPVPDKGDNAWIMICAALVILMSIPGLALFYGGLVRAKN
                     MLSVLMQVFVVFSLISVLWVVYGYSLAFTEGNAFFGTLDKLFLNGITIESVGATFTKG
                     VVISELIFVIFQGAFAAITCGLIVGSFAERAKFSAILVFMVIWFTLSYLPMAHMVWYW
                     AGPDAYTDAAAAEAAGATAGFLFQKGALDFAGGTVVHINAAIAGLVGAYMIGRRTGLG
                     SVAMAPHSLTFTMIGASLLWFGWFGFNAGSALEASGGSALAMVNTWVATSCATLSWMF
                     AEWIFKGKPSMLGAASGAVAGLVAITPAAGFVGVMGAIVIGLLAGIVCLWGVNGLKRL
                     LGADDSLDVFGVHGVGGILGAILTGVFADPALGGTGVYDYVANAVGSYDMVAQVTAQA
                     WGVGTVIVWSGVVSLVAYKLVDIAIGLRVPEDEEREGLDLTSHGESAYHH"
     gene            5088..>5714
                     /locus_tag="PLN02_01905"
     CDS             5088..>5714
                     /locus_tag="PLN02_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI51103.1"
                     /translation="MRIVQISDLHLQSDLLSGGIDPWAALAMALTRLAGLQADALLIS
                     GDIAEAGDSGSYTRLASILAGLPLPLALLPGNHDRRAALHAAFPGHWDNPEMLCRRFD
                     LGPLTLLLLDSLVPGKPWGEIGSAQLHWLDANYPERRRSIVVLHHPPFAVGIPGMDEI
                     GCRCGEGLANWLLRRPQVEAVWCGHVHRPVFTCFAGKMAATAPSTVHQI"
BASE COUNT          889 a         1761 c         1939 g         1125 t
ORIGIN      
        1 cagcgcgagc gcgccgagga tcacccgacg gcgcccgaag cggtcggcaa tcgcgccgtg
       61 ccacagggtc atcgcggcga aggagaaaag gtaggcggac agcgtctgtt gcacctcgat
      121 ctgcgtcgcg cccaggcgtt gggcgatgtc cgggaaggac ggcaggtagg tgtcgatcga
      181 gaacggaccg agcgcggaca ggcaggcgag cagcagtgcc aggccgcggg tactgcggga
      241 ggatgggggc atggaatcag tcggcggcga agcggcggta ttggatcgct tcggcaatat
      301 gggcagccgt gatgatatcg ctttccgcca ggtcggcgac ggtgcgcgcc acgcgcagca
      361 cgcggtgcca ggcgcgcgcc gacaggccga gccggctggc cgcttgctgc agcagggcgc
      421 ggccggcgtt gtcgggctga caatgggtat cgaagccgac ggcgtcgagg cgggcgttgg
      481 cgctgtcctg gcgcttttgc tggcgggccc aggcgcggtt gacccgctcc cggaccaccg
      541 ccgagctttc gccgctgggc gttccgctca gcgcttcgcc gggcagggcg gggacctcga
      601 tgatcaggtc gatgcggtcg agcagcgggc cggacaattt tccccggtag cgggcgacct
      661 gctcgggcgt gcattggcag cgggcgtcgc cgtggtagcc gcagggacag gggttcatcg
      721 ccgccaccag ctggaagcgg gcgggaaact cggcctggcg ggcggcgcgg gcgatgtgga
      781 tgcggccggt ttcgagcggc tcgcgcaggg tttccaggac tttgcggtcg aattcgacga
      841 tttcgtcgag gaacagcacg ccgtggtggg ctaggctgat ttcgccgggg cgcggcgggt
      901 taccgccgcc gaccagcgcc gccgccgacg cggtgtggtg cggactacgg aaaatgcgct
      961 ggcgatagcg ttcggcgcgg aactggccga ccagcgacag cacggcggcc gatgctcttg
     1021 cttcgccgtc gctcaacgcc ggcagcaggc cgggtaaacg ggcggcgagc atcgacttgc
     1081 cggcgccggg cgggccaatc atcaatagcg aatggcgccc ggcggcagcg acttcgagcg
     1141 cccgtcgcgc ctgcgcctgg ccgcgcacgt cggcgaggtc gggcggggcg tccgcgggcg
     1201 ctgccgtggt cggcgccggt ggtgtcagcg gcgtctgccc ggcgaggtgg gcgcagacgg
     1261 cgagcagtga agacgcggcg aggatgtcgc cctgcccggt cagcgccgct tcgccggcgc
     1321 tcgccgcagg caggatgaaa gtcttgccgt cgccggctgc ctgcagggcc atcgccagcg
     1381 cgccgcgtac cgggcgcagt tcgccggaga gcgagagttc gccagcgaat tcgtggccgt
     1441 tcaggctggc cgccggaatc tgcccgctgg cggcgaggat gccgagggcg atgggcaggt
     1501 cgaaacggcc ggattccttg ggcaggtcgg ccggcgccag gttcacggtg atgcggcggg
     1561 ccgggtattc gaagccggaa ttgacgatcg cggcacggac gcggtcgcgc gcttcgcgca
     1621 cttcggtgtc gggcaggccg accagggtca gtccgggcag gccgttggcc aggtgcacct
     1681 cgaccgtgac cggcggcgca tcgaggccgt cgagtccccg gctgagggcg acggccagcg
     1741 ccatgaattt attgcgggct gtcggtgcgc gccttttcca gcgcctcgac gcgggcttcc
     1801 agcgcctgca ggcgctcgcg ggtgcgggcc agcaattgcg cctggatgtc gaattcctcg
     1861 cgggtgacca ggtcgagctt gccgaacagg ccgccgagca gcgccttggc attcttctcg
     1921 atgtccttgg ccggcgaatt ggcgagcagg gcactcatct tgttgccgaa atcttccagg
     1981 atcttggggt cgagcatggt tttcctccgt ggccggcgag tttagcacag cggtatttgc
     2041 tgcgttttac cccgtcgctc gtcgctggtg cgtaacggag tgtaactgca ccgccttggt
     2101 gctctgtttc ggtgcgcatt cctggtgcgg cgcagcaagt caatgaaaaa gcagggaaat
     2161 gtgatgttgg cacgggtcat gcattagtgg cagtacaaaa cgttttcctt ttcaattttc
     2221 ctttacagga gagacaccat gaagaaatcc ctgatcgccg cggcattgct tggcgctttc
     2281 gcagcacccg gtgcgatgca ggttgcgatg gccgacgaag ccagcccgca caccatcacc
     2341 ggcaacctga cgctggccag cagctaccgt ttccgcggta tcgaccagac tttcggcaag
     2401 ccgacgctgc agggtggtat cgattactcc cacgccagcg gtttctacgc cggtaactgg
     2461 aactcgaacg tttcgtcggg tgcgggcttt gccgaaggta acctggaaat ggacttctat
     2521 ggtggttatc gcaccgcctt cggcgacgtt ggtgtcgatg ttggcggcat ctattactac
     2581 tatccgggct cccattccca gccgctgtcc ggcaccaatg ccaagcatcc gacttcgaac
     2641 gccagcggta ccgtggacaa cactgaactg tatgttgccg ccagctacaa gttcctgagc
     2701 gcgaagtaca gctactccgt cggcgattat ttcaatctgc cgaagtccaa gggcaccagc
     2761 tatctcgaat tgaacgccaa ctacgatctg ggtaacggtt ggggtgtcaa tggtcacgtc
     2821 ggtcacttgt tcccgagcgg ctacaagtac aacaccgctg gctcgcagag catttcctac
     2881 accgactgga aagttggcgt gaccaaggat gtcagcggct ggctggttgg tttggcctat
     2941 gttggtacca atgccaatgg ttcttgcagc agcggcgagt tctactgctt cgccagttcg
     3001 ctggacaagt ccggtgccct gggtagcaag acgaaggatg ccggtcgtgg catcgccgtc
     3061 ctgtcggtga ccaagtcctt ctgattcatc agtcatctct ccgccgttgc acgggcccgg
     3121 gcggcgggga gataaaaaaa gtttcaacca gttaggggaa cactcatgaa attcgttact
     3181 gccatcatca agccgttcaa gcttgacgaa gtgcgtgagg cgctgtccgc catcggcgtg
     3241 cagggcatca cggtaaccga ggtcaagggt ttcggccgcc agaaaggtca taccgagctg
     3301 tatcgcgggg ccgagtacgt cgtcgatttc ctgcccaagg tgaagctgga agctgccgtc
     3361 aaggccgacc agatcgacca ggtcatcgaa gccatcgaga agtccgccag caccggcaag
     3421 atcggtgacg gcaagatctt cgtcttcgat cttgaacaag tcgtccgcat ccgcaccggc
     3481 gagaccggcg gcgatgccct gtaaggagtc gtgacatgaa acgaattttc gcaatgctgg
     3541 ccctgctggg cgcggtgacc ttcggggcgc cggcctgggc cgaggaaaag gcggcggatg
     3601 ccgcgcccgt ggccgctgcc gttgccgaag cgccggccgc tgaagccgct gctcccgctc
     3661 ctgccgctga agccgccgct ccggcggaag cgccggccgc tgcggcgccg gttcccgaca
     3721 agggcgacaa cgcctggatc atgatctgtg ccgcattggt gatcctgatg tcgatccccg
     3781 gcctggcgct cttctacggc ggtctggtgc gcgcgaagaa catgctgtcg gtactgatgc
     3841 aggtcttcgt cgtgttctcg ctgatctccg tcctttgggt ggtctatggc tactcgctgg
     3901 ccttcaccga gggtaacgcc ttcttcggca cgctcgacaa gttgttcctc aacggaatca
     3961 cgatcgagtc ggttggagca accttcacca agggcgtggt gatctcggaa ctgatcttcg
     4021 tgatcttcca gggggctttt gcggccatca cctgcggtct gatcgtcggt tccttcgccg
     4081 aacgcgccaa gttctccgcg atcctggtct tcatggtcat ctggttcacc ctgtcctacc
     4141 tgccgatggc tcacatggtc tggtactggg ccggtccgga cgcctacacc gatgccgccg
     4201 cggctgaagc tgccggtgcc accgctggct tcctgttcca gaagggtgcc ctcgacttcg
     4261 ctggcggtac agtcgtccat atcaacgccg cgatcgccgg tctggtcggt gcctacatga
     4321 tcggtcgccg tactggtctg ggctcggttg ccatggctcc gcactcgctg actttcacga
     4381 tgatcggtgc ctcgctgctg tggttcggct ggttcggttt caacgccggc tcggcgctcg
     4441 aagcttccgg cggttccgcc ctggcgatgg tcaacacctg ggttgccacc tcctgcgcga
     4501 cgctgtcctg gatgtttgcc gagtggatct tcaagggcaa gccgtcgatg ctgggtgccg
     4561 cttcaggcgc cgtcgccggc ctggtggcga tcaccccggc tgccggtttc gtcggcgtca
     4621 tgggcgccat cgtcatcggt ctgctcgccg gcatcgtctg cctgtggggc gtcaatggtc
     4681 tgaagcgcct gctcggtgct gacgactcgc tcgatgtctt cggcgtccat ggcgtcggcg
     4741 gcatcctcgg tgcgatcctg accggtgtct tcgctgaccc ggcgctgggc ggtaccggtg
     4801 tatatgacta cgttgcgaat gcggttggca gctacgacat ggttgcccaa gtgacggccc
     4861 aggcctgggg cgtcggcacc gtgatcgtct ggtcgggggt tgtctccctg gtcgcctaca
     4921 agctggtcga tatcgcgatc ggtctgcggg tgccggaaga cgaagagcgt gaaggtctcg
     4981 acctcacctc gcacggcgaa agcgcctacc accactaagc agattcgtag tctctctccc
     5041 ttcacgggca ccccagggtg cccgcttttt tttgctatgc tgggcccatg cgcatcgtcc
     5101 agatttccga cctgcatctg caatccgatc ttctgtcagg cgggatcgat ccgtgggcgg
     5161 cgctggccat ggcgctgacc cgtcttgccg gtctgcaggc tgacgcgctg ttgatcagcg
     5221 gcgatatcgc cgaagcgggt gattccggct cttacacgcg actggcgtcg atcctggccg
     5281 gtctgcccct gccgctggca ttgttgcccg gcaatcacga ccggcgcgcc gcgctgcacg
     5341 cagctttccc cgggcactgg gacaatcccg aaatgctctg ccgacgcttc gatctggggc
     5401 cgctgacttt gctcctcctc gacagcctgg tgccgggcaa gccttggggc gagatcggca
     5461 gcgcccagtt gcactggctg gatgcgaatt atccggagcg ccggcgcagc attgtcgtcc
     5521 tgcatcaccc tccgtttgcg gtgggcatcc ccggcatgga tgaaataggc tgtcggtgtg
     5581 gcgaggggct ggcgaactgg ctattgcgcc ggccgcaggt cgaggccgtc tggtgcggcc
     5641 acgtgcatcg cccggtgttt acctgttttg ccggaaaaat ggcggcaacg gcgccgtcca
     5701 ccgtgcatca gatt
//
LOCUS       DAOSDX010000052         5697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5058_length_5697_cov_3.132577, whole genome shotgun sequence.
ACCESSION   DAOSDX010000052 DAOSDX010000000
VERSION     DAOSDX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5058_length_5697_cov_3.132577"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLN02_01910"
     CDS             <1..442
                     /locus_tag="PLN02_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51104.1"
                     /translation="WAGGFSGRLAYTGLRAEFAKAYTGNSGVVAAGNRLPGVPSSQLF
                     GELAWKHAASGFQAAIEGVARSRVYVDDRNAARSAPGYAIANLRFAVERNYGAFSVAT
                     FLRIDNLFDREYIGSVVVGDGNGRYYEPAPGRGWLAGVSLKHQF"
     gene            complement(450..1793)
                     /gene="gdhA"
                     /locus_tag="PLN02_01915"
     CDS             complement(450..1793)
                     /gene="gdhA"
                     /locus_tag="PLN02_01915"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOI51105.1"
                     /translation="MRYKSFAEFNQHVAARNPGQPEFHQAVAEVIESLWPFIQNHPRY
                     AEHGLLDRLVEPERVLTFRVSWVDDHGEVRVNRGYRIQHSSAIGPYKGGIRFHPSVNL
                     SILKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGRSPGEVMRFCQAFVAELFRHIGSD
                     TDVPAGDIGVGGREVGFMAGMMKKLSNRADCVFTGKGLSLGGSLIRPEATGYGTVYFA
                     EEMLKQRGRSFDGLRVSVSGAGNVAQYAVEKAMALGARVVTVSDSSGTVVDEDGFSAE
                     KLAELMEVKNHLYGRVSDYAARVGATFLPGEKPWRVPVDVALPCATQNELDVNDARSL
                     LANGAICVAEGANMPSTAEAVKLFEASDVLYAPGKASNAGGVATSGLEMSQNAMRLSW
                     PREEVDARLHRIMVDIHETCLRYGRNAEGKVSYVDGANVAGFVKVADAMLAQGVV"
     gene            complement(1823..2197)
                     /gene="rnk"
                     /locus_tag="PLN02_01920"
     CDS             complement(1823..2197)
                     /gene="rnk"
                     /locus_tag="PLN02_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOI51106.1"
                     /translation="MQQNNLIIRESDFVRLMAIQPPDDLRDELERAIVIPDDSIPADI
                     VTMGSRVRYTEQETGNSRQVEIVYPDEADPAASKVSVFAPVGAALIGLGVGHEIDWDF
                     PGGSSRRLTVVEVIQPAAAAPH"
     gene            2336..3283
                     /locus_tag="PLN02_01925"
     CDS             2336..3283
                     /locus_tag="PLN02_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI51107.1"
                     /translation="MHDINDMLLFAQVVKARSFSGAAARLGISKSRVSKSVARLEATL
                     GVRLLQRSTRRLSLTEVGEAYFEHCDRILDEMAQAENTIGSLHREPRGKLRFSAPVAF
                     STLHVAPALADFMLQYPELSVDMTISDRLVNLVDEGYDLALRIAPAPGDNLVARRLAP
                     IRRKICGSPAYLARWGVPEKPEDLAGHNCLDYTYLTTQGFWHLRGPSGDISVPVAGKL
                     RINDDEALSQAVLGGLGLALLPTFIVGSELQAGRLVEVLPGYVPVERFIYAVHLPNRH
                     LPLKVRAFIDFLQQRFGDEPEWDRPASPGSARRWLGLSR"
     gene            complement(3286..4029)
                     /gene="fnr"
                     /locus_tag="PLN02_01930"
     CDS             complement(3286..4029)
                     /gene="fnr"
                     /locus_tag="PLN02_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HOI51108.1"
                     /translation="MPSPIPLNQEISLTVIKTACSNCNLRELCLPFGLSLDELERLDD
                     LISTRRRIKRGDHLYRAGESFDSIYAIRSGFFKTDVLLEDGRDQVTGFQMAGELLGLD
                     GISTEHHTCNAIALEDSEICAIPFNRLESLSREIHNLQHHFHKVMSREIVRDHGVMML
                     LGTMRAEERLAAFLLNLSQRFTARGFSHAEFYLRMTREEIGSYLGLKLETVSRAFSKF
                     QEEGYIAVQQKHIRILNVSGLKALMNHRC"
     gene            4115..5518
                     /gene="hemN"
                     /locus_tag="PLN02_01935"
     CDS             4115..5518
                     /gene="hemN"
                     /locus_tag="PLN02_01935"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOI51109.1"
                     /translation="MNFATENLVFDPQIIRRFDVNGPRYTSYPTADRFVEAFDAEAAA
                     LWLSKRNIGGISRPLSLYFHIPFCNTICYYCACNKIITKDHGRSAKYLKYLGKELALQ
                     SAALEGSEGEHEVIQLHWGGGTPTFLSHDEMRQLMAETRRHFKLLDGGEYSIEVDPRK
                     VDTATVALLGELGFNRMSVGVQDFDERVQVAVNRVQTEEETANVISAARANGFKSVSI
                     DLIYGLPFQSVMGFNRTLERVLAMDPDRLSIYNYAHLPGLFKPQRRIADADLPNADTK
                     LQILALAIKKLTDAGYVFIGMDHFAKPDDELAVAQRQGRLHRNFQGYSTYADCDMLSF
                     GISSISKVGPTYYQNVKTLDEYYDRLDAPMLPVVRGIELTADDILRRSIIQALMCHFE
                     LSIESIESSHLIDFHKYFAAEIEDLREMERGGLLKIERDWITVLPPGRMLVRVIAMAF
                     DRYLRADRQRTRYSKVI"
     gene            5583..>5697
                     /locus_tag="PLN02_01940"
     CDS             5583..>5697
                     /locus_tag="PLN02_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOI51110.1"
                     /translation="MPDSGFLALFLVGLLGGAHCVGMCGGIVGALSLQTAQG"
BASE COUNT          949 a         1787 c         1897 g         1064 t
ORIGIN      
        1 ctgggccggc ggtttctccg gccggctcgc ctacaccggt ctgcgtgccg agttcgccaa
       61 ggcctatacc ggcaacagcg gcgtcgtcgc tgccggcaac cgcctgcccg gcgtgccgtc
      121 ctcgcagctg ttcggcgagc tggcctggaa acatgcagcg agcggcttcc aggcggcaat
      181 cgaaggtgtc gcgcgcagcc gggtctatgt cgacgaccgg aacgcggcgc gctcggcgcc
      241 cggctacgcg atcgccaacc tgcgcttcgc cgtcgagcgc aactacggcg ccttctcggt
      301 cgctaccttc ctgcgcatcg acaacctgtt cgaccgcgag tatatcggtt cggtggtggt
      361 cggcgacggc aacggccgct actacgaacc ggcgcccggc cgcggctggc tggccggggt
      421 gagcctgaag caccagttct gagcgcggct cagacgacgc cctgggcgag catcgcgtcg
      481 gcgaccttga cgaagccggc gacgttggcg ccatcgacgt agctgacctt gccttcggcg
      541 ttgcggccgt agcgcaggca ggtttcgtgg atgtcgacca tgatccggtg cagccgggcg
      601 tcgacttcct cgcgcggcca ggagaggcgc attgcgttct ggctcatttc cagaccggaa
      661 gtggcgacgc cgccggcgtt gctcgccttg cccggggcgt agagcacgtc gctcgcttcg
      721 aacagcttca ccgcctcggc ggtcgacggc atgttggcgc cttcggcgac gcagatcgcg
      781 ccgttggcga gcaggctgcg ggcatcgttt acgtcgagtt cgttctgggt cgcgcagggc
      841 agggcgacgt cgaccggcac ccgccacggc ttctcgccgg gcaggaaagt ggcgccgacg
      901 cgcgccgcgt agtcgctgac gcggccgtac agatggttct tcacctccat cagttcggcg
      961 agtttttcgg cgctgaagcc gtcctcgtcg accaccgtgc cgctcgaatc ggagacggtg
     1021 acgacgcggg cgccgagcgc catcgccttc tcgaccgcgt actgggcgac gttgccggcg
     1081 ccggagacgc tgacgcgtag cccgtcgaaa ctgcgaccgc gctgcttgag catttcctcg
     1141 gcgaaataga cggtgccgta gccggtcgct tccgggcgga tcagcgaacc gcctaagctc
     1201 aggcccttgc cggtgaacac gcagtcggcg cggttcgaga gcttcttcat catgccggcc
     1261 atgaagccga cctcgcggcc gccaacaccg atgtcgccgg ccggcacgtc ggtgtcgctg
     1321 ccgatgtggc ggaacagttc ggcgacgaag gcctggcaga agcgcatcac ttcacccggg
     1381 ctgcgcccct tcgggtcgaa atcggaaccc cccttgccgc cgcccatcgg cagcgtggtc
     1441 agcgcgttct tgaaagtctg ctcgaaggcg aggaatttca ggatcgacag attgaccgag
     1501 ggatggaaac ggatgccgcc cttgtagggg ccgatcgccg acgaatgctg gatacggtag
     1561 ccgcgattga cccgcacctc gccgtgatcg tccacccagg agacgcggaa ggtgagtacc
     1621 cgctccggct cgaccaggcg gtcgagcagg ccgtgttcgg cgtagcgggg gtggttctgg
     1681 atgaacggcc agaggctctc gatgacctcg gcgaccgcct ggtggaactc gggttgcccg
     1741 gggtttcgtg cggcaacatg ttgattgaat tctgcgaatg acttgtagcg cacgctgcgg
     1801 gctccctggt tttttgtctg gatcagtgtg gtgcggcggc ggccggctgg atgacctcga
     1861 ccacggtcag ccggcggctg ctgccgccgg gaaaatccca gtcgatttca tggccgacgc
     1921 cgaggccgat cagcgcggcg ccgaccgggg cgaagacgga gaccttgctg gccgccgggt
     1981 cggcttcgtc gggatagacg atctcgacct ggcggctgtt gccggtttcc tgttcggtat
     2041 agcggacgcg cgaccccatt gtgacgatat ccgccggtat tgagtcgtcg gggataacaa
     2101 tggcgcgctc aagctcgtcg cgcagatcgt cgggcggctg gattgccatc aggcgaacga
     2161 aatcgctctc cctgatgatc aggttgttct gctgcattta tctgaatttt ctagtgattt
     2221 ttgttgatcg gttttttgtg tcgtgggatt gcggatggta aaaggaagtc caaggcttga
     2281 taagccggcg actttgcaca agactgtttc cttttgaata acaattggag cgcgcgtgca
     2341 cgacatcaac gacatgctgc tgttcgccca ggtggtcaag gcgcgcagtt tttccggggc
     2401 ggcggcgcgc ctgggaattt ccaagtcgcg cgtcagcaag tcggtggcgc ggctcgaagc
     2461 gacgctcggc gtccgcctgc tgcaacgcag tacgcgtcgc ctcagtctga ccgaggtcgg
     2521 cgaggcctat ttcgagcatt gcgaccgcat cctcgatgaa atggcgcagg ccgagaacac
     2581 gatcggcagc ctgcaccgcg agccgcgcgg caagctgcgc ttcagcgcac cggtcgcctt
     2641 cagcaccctg cacgtggcgc cggcgctggc cgatttcatg ctgcagtacc cggaactgtc
     2701 ggtggatatg acgatcagcg accgcctggt caatctggtg gacgagggct acgacctggc
     2761 gttgcgcatc gccccggcgc ccggcgacaa cctggttgcc cgccggctgg cgccgatccg
     2821 tcgcaagatc tgcggcagcc cggcctacct ggcgcgctgg ggcgtaccgg aaaagccgga
     2881 ggatctggcc gggcacaact gcctcgacta tacctatctg acgacgcagg gcttctggca
     2941 cctgcgcggc cccagcggcg acatctcggt gccggtggcg ggcaagctgc gcatcaacga
     3001 cgacgaggcg ctatcgcagg cagtgctggg cgggctgggg ctggcgctgc tgccgacctt
     3061 catcgtcggc agcgagctgc aggccgggcg tctggtcgag gtgctacccg gctacgtgcc
     3121 ggtcgagcgc ttcatttacg cggtgcatct gccgaaccgg cacctgccgc tcaaggtgcg
     3181 cgccttcatc gatttcctgc agcagcgctt cggcgacgag ccggaatggg atcgcccggc
     3241 atcgccgggc agtgcgcgcc gctggctggg gctgagccgt taggctcagc agcggtggtt
     3301 catcagcgcc ttcaggccgc tgacgttgag gatgcggatg tgcttctgct ggaccgcgat
     3361 gtagccttct tcctgaaatt tggagaaggc gcgcgacacg gtttccagct tgaggccgag
     3421 atagctgccg atttcctcgc gcgtcatgcg caggtagaac tcggcgtgcg agaagccgcg
     3481 cgccgtgaag cgctgcgaca ggttgagcag gaaggcggcc agccgctctt cggcgcgcat
     3541 cgtgccgagc agcatcatga cgccgtggtc gcggacgatt tcgcggctca tcaccttgtg
     3601 gaagtgatgc tgcaggttgt ggatctcgcg cgacaggctt tccaggcggt tgaacgggat
     3661 cgcgcagatt tcgctgtctt ccagcgcgat ggcgttgcag gtgtggtgtt cggtgctgat
     3721 gccgtcgagg ccgagcagtt cgccggccat ctggaagccg gtgacctggt cgcggccgtc
     3781 ctcgagcagg acgtcggtct tgaagaagcc gctgcggatc gcgtagatcg agtcgaagga
     3841 ctcgccggcg cggtacaggt gatcgccgcg cttgatccgg cggcgggtcg aaatcaggtc
     3901 gtcgagacgc tccagttcat cgagactgag accgaaggga aggcagagtt cccgcaggtt
     3961 gcagttggaa caagccgtct tgatgacggt cagcgaaatc tcctgattca gcgggattgg
     4021 gctgggcatt ggcaacctgc gttggcttga tccatgtcaa ccggaaatgc gatccatccc
     4081 cacataatcg ggccaaaact tggcgccatg ttcaatgaat ttcgcaactg aaaacctcgt
     4141 cttcgaccca cagatcattc gccgtttcga cgtaaacggc ccccgctaca cctcgtatcc
     4201 cactgccgac cgcttcgtcg aggctttcga cgccgaggct gccgctctct ggctcagcaa
     4261 gcgcaacatc ggcgggatca gccgaccgct ctcattatac ttccacatcc ctttctgcaa
     4321 caccatctgt tattactgcg cctgcaacaa gatcatcacc aaggatcacg ggcgcagcgc
     4381 caagtacctg aagtacctgg gcaaggaact ggcgctgcag agcgccgcgc tggaaggcag
     4441 cgaaggcgag catgaggtga tccagctgca ctggggcggc ggtacgccga ctttcctgtc
     4501 gcacgacgag atgcgccagt tgatggcgga gacgcgccgg cacttcaagc tgctcgacgg
     4561 cggcgagtat tcgatcgagg tcgatccgcg caaggtcgat acggcgaccg tggcgctgct
     4621 aggcgagctc ggtttcaacc ggatgagcgt cggcgtccag gatttcgacg agcgcgtgca
     4681 ggttgcggtc aaccgcgtgc agaccgagga agaaacggcc aatgtgatca gcgcggcacg
     4741 cgccaacggt ttcaaatcgg tgtcgatcga cctcatctac ggcctgccct tccagagcgt
     4801 gatgggtttc aaccggacgc tggaacgcgt cctggcgatg gacccggatc gcctgtcgat
     4861 ctacaactac gcgcacctgc ccgggctgtt caagccgcag cggcggatcg ccgacgccga
     4921 cctgccgaac gccgacacca agctgcagat cctggcgctg gcgatcaaga agctgaccga
     4981 cgccggttac gtcttcatcg gcatggacca cttcgccaag cccgacgacg agctggcggt
     5041 ggcgcagcgc caggggcggc tgcaccgcaa cttccagggc tattcgacct acgccgactg
     5101 cgacatgctg tccttcggca tctcgtcgat cagcaaggtc ggtccgacct actaccagaa
     5161 cgtcaagacg ctcgacgagt actacgaccg gctcgacgcg ccgatgctgc cggtggtgcg
     5221 cggcatcgag ctgaccgccg acgacatcct gcggcgttcg atcatccagg cgctgatgtg
     5281 ccatttcgag ctgtcgatcg aaagcatcga gagttcgcac ctgatcgact tccacaagta
     5341 tttcgcggcc gagatcgagg atttgcgcga gatggaacgc ggcggtctgc tcaagatcga
     5401 acgcgactgg atcaccgtgc tgccgccggg gcgcatgctg gtccgtgtca tcgccatggc
     5461 cttcgaccgc tacctgcgcg ccgaccggca gcggacgcgt tactccaagg tgatctgagc
     5521 ggcgtgtcag gaaggcccgg ggcgggttac actgcgggct ttcctgtcgc aagccttgcc
     5581 ccatgcctga ttccggcttc ctggcgctgt ttctcgttgg tctgctcggt ggtgcgcact
     5641 gcgtcggcat gtgcggcggc atcgtcggcg cgctgtcgct gcagacggcg caggggg
//
LOCUS       DAOSDX010000053         5695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5063_length_5695_cov_4.021809, whole genome shotgun sequence.
ACCESSION   DAOSDX010000053 DAOSDX010000000
VERSION     DAOSDX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5063_length_5695_cov_4.021809"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLN02_01945"
     CDS             <1..501
                     /locus_tag="PLN02_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOI51111.1"
                     /translation="GASVFAATMVLLYLTSTLYHAFPAGKLKRLFQKLDHGAIYCFIA
                     GSYTPFALGALAGSWGWTLFGLVWSMAAAGIALKAFNRLSHPWLSTGLYLAMGWLVLI
                     AAVPLVEHVSLPGVILLVAGGVAYTAGVVFFMLDSRVRYAHAVWHAFVATGTGCHFFA
                     VLHYAA"
     gene            579..2777
                     /locus_tag="PLN02_01950"
     CDS             579..2777
                     /locus_tag="PLN02_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC domain/YcaO domain-containing protein"
                     /protein_id="HOI51112.1"
                     /translation="MEIKVNFLDKLRQEAKFDDFTVIADQSIRYKGDGSAPGPFDYFL
                     ASSALCAAYFVKLYCDTRNIPTEHIRLSQNNIVDPGNRYKQTFKIQVELPPDISAKDR
                     LGILRSIDRCTVKKVVQTGPEFVIEEVENLDADAQALLTLNPEAGTQTFIAGKDLPLE
                     QTIANMSGLLAGLGIKIEIASWRNLVPNVWSLHIRDAHSPMCFTNGKGATKESALASA
                     LGEYIERLSCNHFYNDQYWGEEFANAPFVHYPNERWFKPGKKDALPKGLLDDYTLGIY
                     NPDGELRASHLFDTNSGNTERGVCALPYVRQSDGETVYFPTNLIDNLYLSNGMSAGNT
                     LAEAQVQCLSEIFERAVKREILEGEIALPDVPAEVLAKYPGIVAGIEELEKQGFPVLV
                     KDASLGGQFPVMCVTLMNPRTGGVFASFGAHPSLEVALERSLTELLQGRSFEGLNDLP
                     RPTFESNAVTEPNNFVEHFIDSSGVVSWRFFSAKANYDFVEWDFSGHGENSNADEAAT
                     LFGILAEMGKEVYVAVYDQLGANACRILVPGYSEIYPVDDLIWDNTNKALAFREDILN
                     LHRLDDAGLEALLERLEESELDDYTDIITLIGVEFDENTDWGQLTILELKLLINLALQ
                     DFEAAKEQVEAYLQYNENTAERGLFYQAVNVVLEVLLDDELELEDYEPNFRRMFGNER
                     MDAVLGSVDGSIRFHGLTPTSMKLEGLDRHQRLIDSYRKAHAARAKAAGF"
     gene            complement(2806..>5695)
                     /locus_tag="PLN02_01955"
     CDS             complement(2806..>5695)
                     /locus_tag="PLN02_01955"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOI51113.1"
                     /translation="NNVQFADGEWRRFVETWLDKPSDGIVEKTRKIHDDYVHDFVFDD
                     GHIQNIYLLDKKNIARNKVQVIRQFEQTGSHANRYDVTILVNGLPLVQVELKKRGVAI
                     REAFNQVHRYSKESFNSEHSLFKYLQLFVISNGTDSRYFANTTQRNKNSFDFTMNWAK
                     ADNTLIKDLKDFTATFFQKKTLLNVLLHYSVFDASNTLLVMRPYQIAATERILWKINA
                     AWQAKNWSNTESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVFFVVDRKDLDYQTM
                     KEYQRFSPDSVNGSDSTAGLKRNLDKDDNKIIVTTIQKLNNLMKGESDLPIYGKQVVF
                     IFDECHRSQFGEAQKNLKKRFKKFYQFGFTGTPIFAGKNALGAEDTASVFGRELHAYV
                     ITDAIRDEKVLKFKVDYNDVRPQFKAVESEQDEQRLNAAENRQALLHPDRIREITQYI
                     LNNFRQKTHRLQAGAKGFNAMFAVSSVEAAKLYYASFKDLQKNSDKPLKVATIFSFAA
                     NEEQNAVGDIQDESFDVSAMSVSAKEFLSAAIADYNALFKTNFSVDSNGFQNYYRDLA
                     KQVKAKEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAYSRTNRIFDATKTFG
                     NIVTFRDLEHATIDAITLFGDKNTKNVVLEKSYKEYMEGFTDVLTGEARCGFVDVVRE
                     LEQRFPDPSAIEKESDKKAFVKLFGEYLRVENVLQNFDEFASLKALQGVDMNDPAAVE
                     AFKAQRYLSDDDLAALQAIKIPAERKIQDYRSTYNDVRDWLRREKSSAEKEKSTIDWD
                     DVVFEVDLLKSQEINLDYILELIFEHNKKIKDKAALIEDVRRVIRASLGNRAKESLLV
                     DFINQTDLDQIGDKASVIDAFFTFAQAEQQREAQELISDESLNAEAARRYITTSLKRE
                     FASDNGTELNAVLPKMSPLNPQYLTKKQSVFQKIAAFVEKFKGVGGVV"
BASE COUNT         1060 a         1770 c         1624 g         1241 t
ORIGIN      
        1 ggggccagtg tgtttgcggc gacgatggtt ctcctttacc tgacctcgac gctctaccac
       61 gccttcccgg cgggcaagct gaaacgcctg ttccagaagc tggatcacgg cgccatctac
      121 tgcttcattg ccggcagcta caccccgttc gcactcggcg ccctggccgg ttcctgggga
      181 tggacgctgt tcggtctggt ctggtcgatg gccgccgccg gcatcgccct gaaggcattc
      241 aaccggctct cccatccctg gctgtccacc ggcttgtatc tggccatggg ctggctggtg
      301 ctgatcgcgg cggtgccgct ggtcgagcac gtctcgctgc ccggcgtcat cctgctcgtc
      361 gccggtggtg tcgcctacac cgccggtgtc gtcttcttca tgctcgattc ccgcgtccgc
      421 tacgcccatg ccgtatggca cgctttcgtt gcaacgggta ccggctgcca cttcttcgcg
      481 gtgctgcatt acgcggcctg agcggcttca cctcgtaccc ggccgccggg aaacccggat
      541 aatggcgacc gattcgttta gaacgctggg acagccgcat ggaaatcaag gtcaattttc
      601 tcgacaagct tcgtcaggaa gccaaattcg acgacttcac ggtcatcgcc gaccagtcga
      661 tccgctataa aggcgacggc tcggcgccgg ggccgttcga ttacttcctg gcttcgtcgg
      721 ccttgtgcgc ggcctatttc gtcaagctct actgcgacac gcgcaacatc ccgaccgagc
      781 acatccgcct gtcgcagaac aacatcgtcg atccgggaaa ccgctacaag cagactttca
      841 agatccaggt cgagctgccg ccggacattt ccgcgaagga ccgcctgggc attttgcgct
      901 ccatcgaccg ctgtaccgtc aagaaggtcg tccagaccgg ccccgagttc gtcatcgagg
      961 aagtcgagaa tctggatgcc gacgcgcagg cgctgctgac cctcaacccg gaggccggca
     1021 cgcagacttt catcgccggc aaggacctac cgctcgaaca gaccatcgcc aacatgtccg
     1081 gcctgctcgc cgggctgggc atcaagatcg agatcgcctc ctggcgcaac ctggtgccca
     1141 atgtctggtc gctgcacatc cgcgacgcgc attcgccgat gtgcttcacc aacggcaagg
     1201 gcgcgaccaa ggaaagcgcg ctggcttccg ccttgggtga gtacatcgag cgcctgagct
     1261 gcaaccattt ctacaacgac cagtactggg gcgaggaatt cgccaacgcg cccttcgtgc
     1321 attacccgaa cgagcgctgg ttcaagccgg gcaagaaaga cgcgctgccc aagggcctgc
     1381 tcgacgacta cacgctggga atttacaacc cggacggcga gctgcgcgcc tcgcacctgt
     1441 tcgacaccaa ctccggcaac accgaacgcg gcgtctgcgc gctgccctac gtgcgccagt
     1501 cagacggcga gacggtctat ttcccgacca acctgatcga caacctctac ctcagcaacg
     1561 gcatgagcgc cggcaacacg ctggccgaag cgcaggtgca gtgcctgtcg gaaatcttcg
     1621 agcgggccgt caaacgcgaa atcctcgaag gcgaaatcgc gctgcccgac gtgccggccg
     1681 aggtactggc caaatacccc ggcatcgtcg ccggcatcga ggaactggag aagcagggct
     1741 tcccggtgct ggtcaaggac gcctcgctcg gcggccagtt cccggtgatg tgcgtgaccc
     1801 tgatgaaccc gcgcaccggc ggcgtcttcg cctccttcgg cgcccatccc agcctggaag
     1861 tcgcgctcga acgcagcctc accgaactgc tgcaggggcg cagctttgaa ggcctgaacg
     1921 acctgccgcg gccgaccttc gagagcaacg ccgtcaccga gccgaacaac ttcgtcgagc
     1981 acttcatcga ctccagcggc gtcgtctcct ggcgcttctt cagcgccaaa gccaattacg
     2041 acttcgtcga atgggatttc tccggtcacg gcgagaactc caacgccgat gaggccgcca
     2101 cgctgttcgg catcctcgcc gagatgggca aggaggtgta cgtcgccgtc tacgaccagc
     2161 ttggcgccaa cgcctgccgc atcctggtgc ccggctactc ggaaatctac ccggtcgacg
     2221 acctgatctg ggacaacacc aacaaggcgc tggccttccg cgaagacatc ctcaacctgc
     2281 accgcctcga cgacgccggt ctggaagcct tgctggagcg cctggaagag agcgaactcg
     2341 acgactacac cgacatcatc acgctgatcg gcgtcgaatt cgacgagaac accgactggg
     2401 gccagctgac catcctcgaa ctcaagctcc tgatcaacct ggcgctgcag gatttcgagg
     2461 cggccaagga acaggtcgaa gcctacctgc agtacaacga aaacaccgcc gaacgcggcc
     2521 tgttctacca ggcggtcaat gtggtgctgg aagtgctgct cgacgacgaa ctggaactgg
     2581 aagactacga gcccaacttc cgccgcatgt tcggtaacga acgcatggac gccgtgctcg
     2641 gctcagtcga tggcagcatc cgcttccacg gcctgacccc gaccagcatg aaactggaag
     2701 ggctggaccg ccaccagcgc ctgatcgaca gctaccgcaa ggcgcacgcg gcgcgggcga
     2761 aggcagcggg cttttagttg ctttggggct gccgccccac tttcatcaaa ccaccccgcc
     2821 cacgcccttg aatttctcga caaacgccgc gattttctga aacacgctct gcttcttggt
     2881 taggtattgc ggattgagcg ggctcatctt gggtaacacg gcattgagct cggtgccatt
     2941 gtcgctggca aactcccgtt tcagtgaagt cgtgatgtag cgtcgggccg cttcggcgtt
     3001 caagctttcg tcactgatca gttcttgtgc ctcgcgttgt tgctccgcct gggcaaaggt
     3061 gaagaaggcg tcgatcacgc tggccttgtc gcctatctga tccaggtcgg tctgattgat
     3121 gaaatcgacc agcaggcttt ccttggcgcg gttgcccagg ctggcgcgaa tcacccggcg
     3181 cacgtcttcg atcaacgcgg ccttgtcctt gatcttcttg ttgtgctcga agatcagttc
     3241 caggatgtag tccagattga tttcctgcga tttcagcagg tcgacctcga agaccacgtc
     3301 gtcccagtcg atggtcgatt tttccttctc ggccgacgat ttctcccggc gcagccagtc
     3361 gcgcacgtcg ttgtaggtcg aacggtaatc ctgaatcttc cgttcggccg gtatcttgat
     3421 cgcttggagc gccgccaggt cgtcgtcgct caaataacgc tgcgccttga atgcctcgac
     3481 cgccgccggg tcgttcatgt ccacgccttg caaagccttc agactggcga attcatcgaa
     3541 attctgtagc acgttctcga cgcgcaaata ctcgccgaac agcttcacaa aggccttctt
     3601 gtcagattcc ttttcgatgg cagacgggtc ggggaaacgc tgctccagct cgcgcaccac
     3661 atccacgaag ccacaccgcg cctcaccggt cagcacatcg gtgaagccct ccatgtattc
     3721 cttgtagctc ttctccagca ccacgttctt ggtgttcttg tcgccgaaca aggtgatggc
     3781 gtcgatggtc gcgtgctcga ggtcgcggaa agtgacgatg ttgccgaagg tcttggtcgc
     3841 gtcaaaaatt cggttggtac gcgaataggc ctgcatcagg ccgtggtagc gcaggttctt
     3901 gtcgacgaac aaggtgttca gcgtcggtgc atcgaagccg gtcaggaaca tgccgaccac
     3961 gatcagcagg tcgatttcct tggctttcac ctgtttggcc agatcgcggt aatagttctg
     4021 gaagccgttg ctatcgacgc tgaaattggt tttgaacagc gcgttgtaat cggcgatggc
     4081 ggcgctcagg aactccttgg cgctgacgct catggcggaa acgtcgaagc tttcgtcctg
     4141 gatgtcgccc accgcattct gttcctcgtt ggcggcgaac gagaagatgg tggccacttt
     4201 cagcggcttg tcgctgttct tctgtaaatc cttgaaggac gcgtagtaca gcttggcggc
     4261 ctccacgctg cttaccgcga acatggcgtt gaagcccttt gcgccggctt gcaggcggtg
     4321 ggttttctgc cggaaattgt tcaggatgta ctgggtgatt tcgcggatgc ggtcgggatg
     4381 caacaaggcc tgccgattct ccgccgcatt gagcctttgc tcgtcctgct cgctttcaac
     4441 ggccttgaac tgcgggcgga catcgttgta gtccaccttg aacttcagca ccttttcatc
     4501 gcggatggcg tcggtgatta cataggcatg taactcccgg ccaaatacac ttgccgtgtc
     4561 ttccgcgccc agcgcattct ttcctgcaaa gatcggcgtg ccggtaaagc caaactgata
     4621 gaacttcttg aacctcttct tcaggttttt ctgggcttcg ccaaactggc tgcgatggca
     4681 ctcgtcgaaa atgaacacca cctgcttgcc gtagatgggc aggtcgcttt cgcccttcat
     4741 caggttgttg agcttctgga tggtggtgac gatgatcttg ttgtcgtcct tgtccaggtt
     4801 gcgcttgagg ccggccgtgc tgtccgaacc attgacgctg tccggcgaaa aacgctggta
     4861 ttccttcatg gtctggtaat ccagatcctt gcggtcgacc acgaaaaaca ccttgtcgat
     4921 gaaatccagt tcggtcgcca gccgtgccgc cttgaagctg gtcagggtct tgccggagcc
     4981 ggtggtgtgc cagatataac cgccgctttc cgtgttgctc cagttcttcg cctgccaggc
     5041 agcgttgatc ttccacagga tgcgttcggt agcggcgatc tggtaggggc gcatcaccag
     5101 cagtgtgttg ctggcatcga agaccgaata atgcagcagc acgttgagca aggtcttttt
     5161 ctggaagaag gtggcggtga agtccttcaa gtccttgatc agcgtgttat ccgccttcgc
     5221 ccaattcatg gtgaagtcga agctgttctt gttgcgctgc gtggtgttgg cgaaataacg
     5281 gctatcggtg ccgttcgaga tcacgaacag ttgcaggtac ttgaacaggg aatgctcgct
     5341 gttgaagctc tccttgctgt agcggtgcac ctgattgaag gcctcgcgaa tcgccacgcc
     5401 gcgctttttc agttccacct gcaccagcgg caggccattg accaggatcg tcacgtcgta
     5461 acgattggca tggctgcccg tctgctcgaa ctgccggatc acctgcacct tgttgcgggc
     5521 aatgttcttc ttgtccagca ggtagatgtt ctggatatgc ccgtcgtcaa agacaaagtc
     5581 gtgcacgtag tcgtcgtgaa tcttgcgggt tttctcgacg atgccgtcgc tcggtttgtc
     5641 cagccaggtt tccacaaagc gccgccactc gccgtcggca aactgcacat tgttc
//
LOCUS       DAOSDX010000054         5639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5121_length_5639_cov_3.265759, whole genome shotgun sequence.
ACCESSION   DAOSDX010000054 DAOSDX010000000
VERSION     DAOSDX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5121_length_5639_cov_3.265759"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PLN02_01960"
     CDS             <1..1058
                     /locus_tag="PLN02_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HOI51114.1"
                     /translation="VISATYTDQLTGDADSLDIELEDTDGRWLDSWYPEKGASLAYRF
                     GYADTPLISAGRFDVDELELDGPPSIVRIRALATGVQDAVRTRKGRAYENTTLAAIAQ
                     RIAKRHKMTLVGKIEPLRIDRVTQYQEGDISFLQRLANQYGYAFKVTENNSRLVFWKT
                     ADLHLQKAIRSYAPEDLASWNFRDKVSDVPAGVEVKHHDTKTKKLVVYEVKDGDTTVV
                     GSTTAGKATSADTVKMTSRAKSQASAESQAEAELDRRLLERTSGEINLEGDPALAAGA
                     NIELVGFGKLSGLYTVTRATHAIVRNAGYTMSLELKRAAPPASAAPATPAQPQGLKVY
                     GMKDGQVEVLGTTVEEK"
     gene            1055..1675
                     /locus_tag="PLN02_01965"
     CDS             1055..1675
                     /locus_tag="PLN02_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HOI51115.1"
                     /translation="MKNDTFDESAPTLKFGTVSAVDEKTMRVRVRLPELDNLRTDWLP
                     VLTRKSLRDKDYWLPDVGEHVAVLLDANGDDGVVLGAIFSDADAVPVISRDKWHRRFD
                     DSAELEYDRASHTLTVRGGIQNVIVEVGETILLKAGTKVTIDVPETECTGNLLVKGKL
                     TYQGGMAGSSESGAAAVIAGDVEVDGDIDASGSIMDSSGNSNHHSH"
     gene            1738..2094
                     /locus_tag="PLN02_01970"
     CDS             1738..2094
                     /locus_tag="PLN02_01970"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOI51116.1"
                     /translation="MTRLADIQSLHWQPAFGGDGVVENLGDLDQAIQVILRTPKGADP
                     LRPEFGSNLHQYIDYPITRARPHIVRESIEAIRTWEKRLTVVDVVVEVVETSTIRVRV
                     VWKVAAGVLNETEVLL"
     gene            2091..3194
                     /locus_tag="PLN02_01975"
     CDS             2091..3194
                     /locus_tag="PLN02_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOI51117.1"
                     /translation="MSTRPKFVDDDSANITQELIAAYEAMTGKTVYPAHVDRVFIDLV
                     AYSKTLVMSSINDVGCQSLLAFSRAPIIDYLGELVGVSRLPAQSARTQVRITFAAPLD
                     TGLAIPAGLRAEAAGSIQFQTEEALIVPAGASSVDLTMVAIEPGTGSNGLLAGQVNNL
                     VDDLGVDVESVGNLVVTAGGSDQESDDRLRERIQLAPESFSVAGSLLAYRHHALRAHP
                     DILDVAVLSPSPGVVNLHPLAKTGLPGAAIMDAVADTTSHKKVRPLTDHVVVLPPVEF
                     AYTITARLMLYDASEPAVVLTAAQDGAAGFASRHQAVLGRDVVPSQIIAALSVPGVYQ
                     VELLSPAALMVVPENGWAHCTGIDIELIGGVNG"
     gene            3187..4038
                     /locus_tag="PLN02_01980"
     CDS             3187..4038
                     /locus_tag="PLN02_01980"
                     /inference="COORDINATES: protein motif:HMM:NF021220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HOI51118.1"
                     /translation="MAEHIPNVLALDPRFGPLAKLAVDAHHADLSKLLIYLIDLVDEQ
                     AIPHLAEQFHVVGEEGWLLATTDQARRELIKRSIEVHRHKGTPWAIERLLESVGARAE
                     LREWWEMDPPGVPHTFEIDVYAGSLPVTGNSPLLGEVATKRMADLINSVKPVRSHYTL
                     RVGASYIGSLAQGAALGHPVAVVTNKGNQPLETHLHGSQSWQSGAALSRPSAVATPTG
                     YQRPDRALSASIPTRPAAVMSRPATIVSPLGGQQPDRMLAATIPTRPCAAMSRPAVFA
                     TAVFRNP"
     gene            4051..>5639
                     /locus_tag="PLN02_01985"
     CDS             4051..>5639
                     /locus_tag="PLN02_01985"
                     /inference="COORDINATES: protein motif:HMM:NF023985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOI51119.1"
                     /translation="MTTLNLEVITTTVGLQAVLDAQNQGITLRLTHIGLGDGAYTPTK
                     QRTALANRRVLLPIASSKINANNYQLDLSAAAQGQEEFWVREVGIYDEAGRLIFVWSH
                     PSQSLGYKSAPSRFLIGLSLVVTEVPLGGITIIDQGQPLNLAIGDIEADLTGEHAAGG
                     SHHYPLTNWIERDWLNEANLWEYHAEIMRGLGQSGLYTCRGYIRSGTESFNRTFDGAY
                     STINLHDHPNYPGMPGLGELSAVINGYYLRTRHNDYLLNAPVDGAYLATAPIAPPAVP
                     ASVTAAGNVAAQVAEMRAYFQALAARDITQRDYRPHFRWTLSVLELWPEILSGDALTD
                     TFGSFRHQEDSASLRKQLEQVLFYANSGHKNRFENVSFIPASLRQVSSTGKPQWVVWR
                     CRIVAADVGSVGDYPIEQLLTAVDRPGERWHYDMTHESMLTSRFARFRVNSSLSTDPA
                     FGATGIAEVLDELMGKVPGFDGPAANLAETYTENGQAITLTQWNSNAPLNAARYNRRY
                     SAPADASNRTSAMRGFNDPSL"
BASE COUNT         1095 a         1915 c         1690 g          939 t
ORIGIN      
        1 acgtgatatc tgctacctac accgaccagc taacgggcga tgcggatagc ctcgacattg
       61 agctggaaga cacggacggg cgctggctgg atagctggta cccggaaaaa ggcgccagcc
      121 tggcctatcg attcggctac gcggatacgc cgctgatctc ggccggccgc ttcgacgtcg
      181 acgaactaga gcttgacggg ccgccgtcga tagtacgcat cagggcgctg gccacgggcg
      241 tgcaagatgc cgtacgcacc cgtaagggcc gcgcctacga aaataccact ctggcggcga
      301 tcgcccagcg catcgccaag cggcacaaga tgacgctggt gggcaagatc gagccgctgc
      361 ggatcgaccg ggtgacgcag taccaggaag gcgatatctc tttcctgcag cgcctggcga
      421 atcagtacgg ctacgccttc aaggtgaccg agaacaactc tcgcctggtg ttctggaaaa
      481 cggccgacct gcacctgcag aaggccatcc gcagttatgc cccggaagac ctggcgagct
      541 ggaatttccg cgacaaggtg tccgacgtac cggccggcgt cgaggtgaag catcacgaca
      601 ccaagaccaa gaagctggtg gtctatgaag tgaaggacgg agatacaaca gtcgtcggca
      661 gcactacggc cggcaaggcc accagcgccg acacggtgaa aatgacgagc cgggcgaaaa
      721 gccaagcctc ggccgagtct caggcagagg ccgagctgga ccggcgcctg ttggaacgga
      781 ccagcggtga gatcaacctc gaaggcgatc ctgccctagc tgctggcgcc aatatcgagc
      841 tagtcggctt cggcaagctg tccggccttt acaccgtcac tcgcgccact catgccatcg
      901 tccgcaacgc gggctacacc atgtcgctgg agctgaagcg ggcggcgccg ccggccagcg
      961 cggcgccggc aacccctgcc cagcctcagg ggctgaaggt ctatggcatg aaggatggcc
     1021 aagtagaggt gttgggtacg acggtggaag aaaagtgaag aacgatacct tcgatgaatc
     1081 agcccccacg ctgaagttcg gcaccgtatc ggccgtcgac gaaaagacga tgcgtgtgcg
     1141 ggttcgcctg cccgaactgg acaacctacg cacggactgg ctgccggtgc tgacccgcaa
     1201 gagcctgcgc gacaaggact actggctgcc cgacgtcggc gagcacgtgg cggtgttgct
     1261 ggatgccaac ggcgacgatg gcgtggtgct gggagccatc ttttcggatg ctgacgcggt
     1321 gccagtgatc agccgtgaca agtggcaccg ccgcttcgac gactcggccg agctggagta
     1381 tgaccgtgcc agccatacgc tgaccgtgcg cggcggcatc cagaatgtga tcgtcgaggt
     1441 cggcgagacc attctgctca aggctggtac caaggtgacg atcgacgtgc cggaaaccga
     1501 atgcaccggc aatctgctgg tcaagggcaa gctcacctac cagggcggca tggctggttc
     1561 cagcgaatcg ggcgcggcgg ccgtcatcgc gggcgatgtc gaagtcgacg gcgatatcga
     1621 tgcctcggga tcgatcatgg acagcagcgg aaacagcaac caccacagcc actagacggc
     1681 gagcgtctat aaacctcatt aatatcccgc gcggctgggc ttggcgatca tgtcgccatg
     1741 actcggctcg ctgacatcca atccctgcac tggcaacccg cattcggcgg tgacggtgtc
     1801 gtcgaaaacc tcggcgacct cgaccaggca atccaggtga tcctgcgcac cccaaaaggt
     1861 gccgatccgc tgcggccaga gttcggttcc aacctgcacc aatacatcga ttaccccatc
     1921 actcgggcgc gcccccatat cgtgcgcgag agcatcgaag ccatccgtac ctgggaaaaa
     1981 cggctgaccg tagtggatgt agtggttgag gtggtggaaa cgtcgaccat ccgggttcgc
     2041 gttgtctgga aagtcgctgc aggggttctg aatgaaacgg aggtgctgct gtgagcaccc
     2101 gaccgaagtt cgtcgacgac gactccgcca acatcactca ggagctgatt gctgcctacg
     2161 aggccatgac gggaaagacc gtttatccgg cccatgtgga tcgggtgttc attgacctgg
     2221 tggcgtacag caaaactctg gtcatgtcgt ccatcaacga tgtcggttgc cagtcgctgc
     2281 tcgccttcag ccgtgccccc atcatcgact acctgggcga gctggtcggc gtatcccgtc
     2341 ttccagccca gagcgcccgc actcaggtgc gcattacctt cgccgcaccg ctcgataccg
     2401 gcctggccat ccccgccggc ttgcgagccg aggcggccgg cagcattcag tttcagaccg
     2461 aggaagcgtt gatcgtgccg gccggcgcct cctcggtcga cctgaccatg gtggccatcg
     2521 aacctggcac cggcagcaac gggctcttgg ccggccaggt taataatctg gtcgacgatc
     2581 ttggcgtcga tgtcgagagc gtaggcaacc tcgttgtgac tgcgggcggc tcagaccagg
     2641 aaagcgatga ccgcctgcgg gaacgcatcc agttggcacc ggagagcttc agcgtggccg
     2701 gcagcctgct ggcctaccgg catcatgccc tacgcgctca tccggacatt ctggacgtgg
     2761 cggtcctgtc gccgtcaccc ggtgtcgtca atttgcaccc gctggcaaag accggcctgc
     2821 ccggcgcggc gattatggat gcggtggccg ataccacttc gcacaagaag gttcggccgc
     2881 ttaccgacca cgttgtggtg ttgccgccgg tcgagttcgc ctacacgatc accgcccgcc
     2941 taatgctgta cgacgcgagt gaacccgctg ttgttctgac tgccgcccag gatggcgccg
     3001 ctggtttcgc cagcaggcac caggcagtcc tggggcggga cgttgtgcca tcgcaaatca
     3061 tcgccgcact gtcggtgcca ggcgtgtatc aagttgagtt gctatccccg gccgctctga
     3121 tggtcgtacc ggaaaacggc tgggcacact gcaccgggat cgacatcgaa ctgatcggag
     3181 gggttaatgg ctgagcacat cccgaacgtc ctggccctcg atccacgctt cggcccgctc
     3241 gctaaactgg ctgtcgatgc gcatcacgcc gatctgtcaa agctgctgat ctacctgatc
     3301 gacctcgtcg acgaacaggc cattccccac ttggctgagc agttccatgt agtcggtgaa
     3361 gaagggtggc tactggcgac caccgaccag gcgcgccgcg agctgatcaa gcgatcgatc
     3421 gaggtgcacc ggcacaaagg aacgccatgg gcgatcgaac gccttcttga atccgtaggc
     3481 gcgagagccg agctgcgcga atggtgggaa atggacccgc cgggcgtacc gcatacattt
     3541 gaaatcgatg tctacgccgg atccctcccc gtcactggca actcgccgct gctcggcgag
     3601 gttgcgacca agcgcatggc ggacctgatc aacagcgtga agccggtgcg cagtcactac
     3661 accctgcgcg tcggcgccag ctatatcggt agcctggcgc aaggcgccgc actcggccat
     3721 cccgtcgccg tcgtcaccaa caaaggcaac cagccgctcg aaacccacct acatggcagc
     3781 caatcctggc agagcggcgc cgccctttcg cgtccttccg ccgtcgctac gccaaccggc
     3841 taccagcgac cggatcgcgc cctgtccgcc tcgatcccga ctcgcccggc ggccgtcatg
     3901 tctcgcccgg ccacgatcgt ctcgccgctc ggcggacaac agccagaccg gatgctcgcc
     3961 gccacgatcc caacccggcc gtgtgccgca atgtcccgcc cggcggtttt cgcaactgcc
     4021 gtttttcgca acccctgacc gaggcaccac atgaccacgc tcaacctcga agtcatcacc
     4081 accaccgtcg gcctgcaggc cgtgctcgat gcccagaacc agggcatcac gctgcgcctc
     4141 acgcacatcg gcctcggtga tggtgcctat actccgacca agcagcgcac cgcgcttgcc
     4201 aaccgccgcg tcctgctgcc catcgccagc agcaagatta acgccaacaa ctatcaactt
     4261 gacctctcgg ccgcggcaca aggccaagaa gaattctggg tccgcgaagt cggcatctac
     4321 gacgaagccg gccggctgat cttcgtctgg tcgcatccga gccagtccct cggctacaag
     4381 tcggcgccga gtcgcttcct gattggtctg agcctcgtcg tcaccgaagt cccgctcggc
     4441 ggcatcacca tcatcgacca gggccagccg ctcaatctcg ccatcggcga cattgaggcc
     4501 gacctcaccg gcgagcacgc cgccggcggc agccatcact acccgctgac caactggatc
     4561 gagcgcgact ggctcaacga ggcgaacctc tgggaatacc acgccgagat catgcgcggc
     4621 ctcggccagt ccggcctcta tacctgccgc ggctacatcc gctccggtac cgaatcgttc
     4681 aaccggacgt tcgacggcgc ctactcgacc atcaacctgc acgaccaccc gaactacccc
     4741 ggcatgcccg gcctcggcga actctcggcg gtgatcaacg gctactatct gcgcacgcgc
     4801 cacaacgact atctgctcaa cgccccggtc gacggcgcct acctcgccac cgcgccgatc
     4861 gcgccgcccg ccgtgccggc cagcgtcacc gccgccggca atgtcgcggc gcaggtcgcc
     4921 gagatgcgcg cctatttcca ggcactggcg gcgcgcgaca tcacccagcg cgactaccgg
     4981 ccgcactttc gctggaccct ctccgtgctg gagctgtggc ccgaaatcct cagcggcgac
     5041 gcgctgaccg acaccttcgg ctcgttccgc caccaggagg attccgccag cctgcgcaaa
     5101 cagctcgaac aggtgctctt ctatgccaac tcgggccaca agaaccgctt cgagaacgtc
     5161 tcgttcatcc cggcaagcct gcgccaggtg agcagcaccg gcaagccgca gtgggttgtc
     5221 tggcggtgcc gcatcgtcgc ggccgacgtc ggatcggtcg gcgattaccc gattgagcag
     5281 ctactcaccg ccgtcgatcg gcccggcgag cgctggcact acgacatgac tcacgagagc
     5341 atgctgacca gccgtttcgc tcgtttccgg gtcaattcga gcctctcgac cgacccggcc
     5401 ttcggtgcca ccggcatcgc cgaggtgctc gacgaactga tgggcaaggt gcccggcttc
     5461 gacggcccgg cggccaacct ggccgagacc tacaccgaaa acggccaggc catcacgctg
     5521 acacagtgga acagtaacgc gccgctcaat gcggcccgct acaaccgccg ctactccgcc
     5581 ccggccgatg ccagcaaccg gacgtcggcc atgcgcggct tcaacgaccc gagcctctt
//
LOCUS       DAOSDX010000055         5615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5159_length_5615_cov_2.947842, whole genome shotgun sequence.
ACCESSION   DAOSDX010000055 DAOSDX010000000
VERSION     DAOSDX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5159_length_5615_cov_2.947842"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /gene="pgk"
                     /locus_tag="PLN02_01990"
     CDS             complement(<1..631)
                     /gene="pgk"
                     /locus_tag="PLN02_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOI51120.1"
                     /translation="MNVIKLTDLDVSGKRVFIRADLNVPQDEAGNITEDTRIRASLPS
                     IRYCLEKGAAVMVTSHLGRPTEGLLNPEDSLMPVAVRLGQMLHLPVRLVADWVDGGFE
                     VKPGEVVLLENCRVNKGEKKNNDELAQKMAKLCDIYVNDAFGTAHRAEATTHGMAKYA
                     PVACAGVLMGAEIDALSKALHEPKRPLVAIVGGSKVSSKLTILRSLADKV"
     gene            complement(648..1682)
                     /locus_tag="PLN02_01995"
     CDS             complement(648..1682)
                     /locus_tag="PLN02_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOI51121.1"
                     /translation="MLRLAINGYGRIGRCFLRALHESSANRRFEVVAINEPANLDSIA
                     YLTRYDSTHGRFPAEIGLGEGRLFIDGRPIAVSHARTPEEVDWGEHGIDLLVECSGAY
                     GRRDELTRFLAAGCPRLLLSHPGHSADDVDATIVAGINQHALDGEQRLVSAASCTTNA
                     IVPVLDLLDREIGVDQALLTTLHSVMNDQPLIDGYHHDDLRRTRSAMQSIIPVSTGLA
                     RGIERLLPQLAGRVHAKAIRVPTLNVSAIDLNVHTRQAVSVKLINRLLGDAAAGPLRG
                     RLACSDQAHASIDFNHDPHSAIVDGSQTRTAGSHLLNLFVWFDNEWGFANRMLEVADH
                     WSRHWTAHQQ"
     gene            complement(1811..2506)
                     /locus_tag="PLN02_02000"
     CDS             complement(1811..2506)
                     /locus_tag="PLN02_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51122.1"
                     /translation="MAQIASASQTAKARRRPVVASTDETAASLSPAMQRIVSLLRAKS
                     PLSPKEIARDAFIALTTLEGGGYLKKMRGLGLIRIEGWLKNHNGFTTPLYALGRGTDC
                     PRPKFASIDRDSRGMAKIVAILNTRSKLDYREIAKLAGISANTIKNAKYMDSLLEQGR
                     VHVAAWRRGRNGHLYPLFNAGADESAVRPEQLSRQEIMQRHRTRQKILSGKATAFDCQ
                     LKSLPLAGPPVTP"
     gene            complement(2522..4147)
                     /locus_tag="PLN02_02005"
     CDS             complement(2522..4147)
                     /locus_tag="PLN02_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI51123.1"
                     /translation="MLNHLSLRARLWLLGIVAALGMLLLAVSSLIFTGRSEVLFTSFV
                     DERIAVRHMATLAYANGLQKGQAIRNILLDPGKQTAYDNFDKAHAVFLQETEKLLPAL
                     ANDAATHDIAPRLKANLERWQPLQKEVIDLVRAGNGEAAKALLVSQETPAWRQVRDDL
                     LEIGKLAETAAATERAELIDGLDWLSKLSIIIGSLSLLLVASIIVLVGRSIYRQIGGE
                     PAVVAIALQRIAQGDLSESLTLIPGDESSVVAAMQRMQGQIRQLIAETVHSADSVVKE
                     SEAMLTEAAQLDKTAEEQSNATAAIAAAVEEFTVSIGVMSDNAGEAGRLSGESENHAH
                     ESLGVVSDATGIIQRVATGMSDAAGTMEELSGKVAAITGIVKTIRDIADQTNLLALNA
                     AIEAARAGESGRGFAVVADEVRKLAERTSVSTGEIGKMVNAIQQSTGQVVSGVAQGVQ
                     LADSSVELAQQAGSAIAEMRAMAQQVAEVISDVDSGLREQSTASTDVAVKIEQIATQA
                     EEASAIAHETSQASTSLDSTARQLQAAVSRFRI"
     gene            complement(4232..>5615)
                     /locus_tag="PLN02_02010"
     CDS             complement(4232..>5615)
                     /locus_tag="PLN02_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51124.1"
                     /translation="PHRDGSIVPTEVVTTVLLDDHGQPTRILGITRDISERRRNEAAL
                     ARHQAELEQRVEERTVALSIAKEAAEAASRAKSTFLATMSHELRTPMNAILGMTDLAL
                     RRASDAKQKDQLGKVADAARHLLAVINDILDLSRIEADRLTLEHIPFQLNEIVAPVQR
                     LLEHLAAEKGLYLVFEVPETLARTQVSGDPHRLKQVLLNLCINAIKFTDCGGVTVHLA
                     LSGEAPHFVLHCSVEDTGIGVTPEIQRRLFEPFVQADGSMTRRYGGSGLGLAICKRLV
                     EMMDGRIGLDSQPGRGSRFWFEARLGGSDALLPLPRNSTLDAIAALERRFAGKRILLA
                     EDEPVNREVFRELLGDAGLLVDTANNGEAAIAMAGSKDYALILMDIQMPGIDGVAASQ
                     AIRGQPGGSELPIIALTANAFSEDRQRCLDAGMDDHISKPVDPERLFATLYRWLAIAD
                     ERNGQAAGAL"
BASE COUNT          848 a         1688 c         1991 g         1088 t
ORIGIN      
        1 cgaccttgtc ggccagcgaa cgcaggatgg tcagtttgga cgaaaccttg gagccgccga
       61 cgatcgccac cagcgggcgc ttcggttcgt gcagcgcctt ggacagggcg tcgatctcgg
      121 cgcccatcag cacgccggcg caggcgaccg gcgcgtactt ggccatgccg tgggtggtgg
      181 cttcggcgcg gtgggcggtg ccgaaggcgt cattgacgta gatgtcgcag agcttggcca
      241 tcttctgcgc cagctcgtcg ttgttcttct tctcgccctt gttgacgcgg cagttctcaa
      301 gcagcacgac ctcgcccggc ttgacctcga aaccgccatc gacccagtcg gcgaccaggc
      361 gcaccggcag gtgcagcatc tggccgagac gcacggcgac cggcatcagg ctgtcttccg
      421 ggttgagcag gccttcggtc gggcgaccga ggtgtgaggt gaccatcacc gcggcgccct
      481 tttccaggca gtagcggatc gacggcagcg aggcgcggat gcgcgtgtcc tcggtgatgt
      541 tgccggcttc gtcctgtggc acgttgaggt cggcgcggat gaaaacgcgc ttgccggaaa
      601 catcgaggtc ggtcagcttg atgacgttca tggtcttctc caaggggtta ttgttggtga
      661 gcggtccagt ggcgcgacca gtggtcggcc acttcgagca tgcggttggc gaatccccat
      721 tcgttgtcga accagacgaa gagattcagc aggtggctgc cggcggtgcg cgtctggctg
      781 ccgtcgacga tcgccgagtg cgggtcgtgg ttgaagtcga tcgacgcgtg cgcctggtcg
      841 gagcaggcca ggcggccgcg cagcgggccg gcggcggcgt cgccgagcag ccggttgatg
      901 agcttgaccg aaaccgcctg gcgggtgtgc acgttgaggt cgatcgccga gacgttcagc
      961 gtcggcaccc ggatcgcctt ggcgtgcacg cggccggcca gttgcggcag caggcgctcg
     1021 atgccgcgcg ccagaccggt cgacaccggg atgatcgact gcatcgccga acgggtgcgg
     1081 cgcaggtcgt cgtggtgata gccgtcgatc agcggctggt cgttcatcac cgagtgcagc
     1141 gtggtgagca gcgcctggtc gacgccgatt tcgcggtcga gcaggtcgag caccgggacg
     1201 atcgcgttgg tcgtgcacga ggcggcggaa accaggcgct gctcgccgtc cagcgcgtgc
     1261 tggttgatgc cggcgacgat ggtggcatcg acgtcatcgg cgctgtggcc cgggtgggag
     1321 aggagcaggc gcgggcagcc ggcggcgagg aagcgcgtca gctcgtcgcg ccggccgtag
     1381 gcgccggagc attcgaccag caggtcgatg ccatgctcgc cccagtcgac ctcctccggc
     1441 gtgcgcgcat ggctgacggc gatcggccga ccatcgatga acaggcgtcc ctcgccgagc
     1501 ccgatttcgg ccgggaaacg gccgtgcgtc gagtcgtagc gggtgaggta ggcgatgctg
     1561 tcgaggttgg ccggctcatt gatggcgacg acctcgaaac gccggttggc cgaagattcg
     1621 tgcagcgcac gcaggaagca gcgaccgatc cggccgtagc cattgatggc gagacgcagc
     1681 acttcagggg acggaaagtt ggcaggcaag tacggtggtg cgacgcggat ggtcaagccg
     1741 caattttacc agcacggcat ggattccggg tacgcgtgac aaatggaggc gcctggctgt
     1801 ccgagctgcc tcatggagta actggcgggc cagccagggg cagggatttc agttggcaat
     1861 caaaggcagt cgcttttccg gagaggattt tttggcgggt tcgatggcgc tgcatgattt
     1921 cctgccggct cagttgttcc ggcctgactg cgctttcgtc ggcgccggcg ttgaagagcg
     1981 ggtagaggtg gccgttgcgg ccgcgccgcc acgctgccac atggactctt ccttgttcca
     2041 ggagcgaatc catgtatttg gcgttcttga tcgtgttggc ggagatgccg gccagcttgg
     2101 cgatttcccg gtagtccagc ttgcttcggg tattgaggat ggcgacgatt ttcgccatgc
     2161 cgcgggagtc ccggtcaatc gacgcgaatt tcggacgggg gcaatccgtt cctcggccca
     2221 gcgcataaag cggcgtggta aaaccgttgt ggtttttcag ccagccttcg atgcgaatca
     2281 gcccaaggcc tctcatcttt ttgaggtatc cgccgccttc gagggtggtc agggcgatga
     2341 aggcgtcgcg cgcgatctcc ttgggagaga ggggggattt tgctcgcagc agcgaaacga
     2401 tccgctgcat cgccggggag agcgaggcgg cggtttcgtc agtgctggcg acgacgggcc
     2461 gccgccgggc tttggccgtt tggctggcgg atgcgatttg agccatcggg catcgccgcc
     2521 atcagatacg gaagcgggac accgcggcct gcaactggcg ggcggtgctg tcgagagagg
     2581 tcgaggcttg cgaagtctcg tgcgcgatgg cgctggcttc ttccgcctgg gtcgcgatct
     2641 gttcgatctt gacggcgaca tcggtggatg cggtcgattg ttcgcgcagg ccgctatcga
     2701 catcgctaat gacctcggcg acctgctgcg ccatcgcacg catctcggcg atcgccgatc
     2761 cggcctgctg cgccagctca acgctgctgt cggcgagctg gacgccctgg gcgacgccgc
     2821 tgaccacctg gccggtcgat tgctggatgg cattgaccat cttgccgatt tctccggtgg
     2881 acaccgaggt gcgctcggcc agcttgcgca cttcgtcggc gacgacggca aagccacgcc
     2941 cgctttcgcc ggcgcgggcg gcttcgatgg cggcgttcag ggcgagcagg ttggtctggt
     3001 cggcaatgtc gcggatcgtc ttgacgatgc cggtgatcgc tgcaaccttg cctgaaagtt
     3061 cttccatggt gccggctgcg tcggacattc cggtggccac ccgctggatg attccggtgg
     3121 catcggagac cacgcccaga ctctcgtggg catggttttc ggactctccg gagaggcgcc
     3181 cggcttcgcc ggcgttatcc gacatgacgc cgatgctgac ggtgaattcc tcgacggcgg
     3241 cggcaatggc tgccgttgcg ttgctttgct cttcggctgt tttgtccagt tgcgccgctt
     3301 cggtgagcat cgcttcgctt tccttgacca cggaatcggc gctatggacg gtttcggcga
     3361 tcagttggcg gatctgtccc tgcatgcgct gcatggcagc gacgacgctg ctctcgtcgc
     3421 cggggatcag ggtcagggat tcgctcaggt cgccctgggc gatccgttgc agggcgatgg
     3481 cgacgacggc gggttcgccg ccgatctgcc gatagatgct gcggccgacc aggacgatga
     3541 tgctggccac cagcagcagg ctgagcgagc cgatgatgat cgacagcttg ctcaaccagt
     3601 cgaggccatc gatcaattca gcgcgctcgg ttgctgcagc cgtctcggcg agcttgccga
     3661 tttccagcag gtcgtcgcgc acctgacgcc aggccggggt ttcctggctc accagcagtg
     3721 ccttggcagc ctcgccgttg cccgcccgga ccagatcgat gacttccttc tgcaaaggtt
     3781 gccagcgctc gagattggct ttcaggcgcg gggcgatatc gtgggtggcg gcgtcgttgg
     3841 ccagggctgg cagcagtttt tcggtttcct gcaggaaaac cgcatgcgcc ttgtcaaagt
     3901 tgtcgtaagc cgtctgtttg ccggggtcga gcaggatgtt gcggatggcc tggcctttct
     3961 gcaggccgtt ggcgtaagcc agcgtcgcca tgtgacgaac ggcgatccgt tcatcgacga
     4021 aggaagtgaa caggacttcg ctacgcccgg tgaaaatgag cgaagagacg gcgagcagca
     4081 gcatgcccag cgcggcgacg atgcccagca gccagagacg agccctgagc gaaagatggt
     4141 tcagcatgtt tcctcctgat gcgatggtcg ggatatttcc cttttctcga catgctaatc
     4201 cagctaggaa acatggcgga agctttgttg ctcagagagc tccggcagct tgtccgtttc
     4261 tttcgtcagc gatggccagc cagcggtaga gcgtcgcgaa caggcgctcc gggtcgaccg
     4321 gcttgctgat gtggtcgtcc atgccggcgt ccaggcaacg ctggcggtct tcgctgaagg
     4381 cgttggcggt cagcgcgatg atcggcagtt cgctgccgcc gggctggccg cggatcgcct
     4441 ggctggcggc gacgccgtcg atgccgggca tctggatgtc catcaggatc agcgcgtagt
     4501 ccttgctgcc ggccatggcg atcgccgctt cgccattgtt cgcggtatcg accagcagcc
     4561 cggcgtcgcc gagcagttcg cggaagacct cgcggttcac cggttcgtcc tcggcgagca
     4621 ggatgcgttt gccggcaaac cggcgctcca gggctgcgat ggcgtccagc gtgctgttgc
     4681 gcggcagcgg caggagcgca tcgctgccgc ccagccgggc ttcgaaccag aagcggctgc
     4741 cgcgaccagg ctggctgtcg aggccgatgc ggccgtccat catctccacc agccgcttgc
     4801 agatcgccag cccgaggccg gagccgccgt agcggcgggt catcgaaccg tcggcctgga
     4861 cgaagggttc gaacagacgg cgctggattt ccggcgtgac gccgatcccg gtatcttcga
     4921 ccgaacagtg caggacgaaa tgcggggcct cgccgctcag cgctaggtgg acggtgacgc
     4981 cgccgcagtc ggtgaacttg atcgcgttga tgcacaggtt gagcagcacc tgcttgagcc
     5041 ggtgcgggtc gccgctgacc tgggtgcggg cgagcgtttc cggtacttcg aaaaccaggt
     5101 agaggccctt ttcggcggcc aggtgttcga gcaggcgctg caccggggcg acgatctcgt
     5161 tcaactggaa gggaatgtgc tccagcgtca ggcggtcggc ctcgatgcgc gacaggtcga
     5221 ggatgtcgtt gatcaccgcc agcaggtggc gggcggcgtc ggcgaccttg cccaactggt
     5281 ccttctgctt ggcgtcgctg gcacggcgca gcgccaggtc ggtcatgccg aggatggcgt
     5341 tcatcggcgt ccgcagctcg tggctcatcg tcgccaggaa ggtgctcttc gcccggctcg
     5401 ccgcttccgc cgcttccttg gcgatcgaca gcgccaccgt gcgctcttcg acgcgttgtt
     5461 ccagttcggc ctggtggcgg gcgagcgccg cctcgttgcg gcgccgttcg gaaatgtcgc
     5521 gggtgatgcc gaggatgcgc gtcggctggc cgtggtcgtc gagcagcacg gtggtgacga
     5581 cttcggtcgg gacgatgctg ccgtcccggt gcggc
//
LOCUS       DAOSDX010000056         5613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5160_length_5613_cov_3.135121, whole genome shotgun sequence.
ACCESSION   DAOSDX010000056 DAOSDX010000000
VERSION     DAOSDX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5160_length_5613_cov_3.135121"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="PLN02_02015"
     CDS             complement(<1..1958)
                     /locus_tag="PLN02_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOI51125.1"
                     /translation="MSISVEAVKTGVRADLLHHDPLLDCLVELTRIHGRPGTRAALVA
                     GLPLEKGVLTPSLFPRAAARAGLSAKLSRRPLERIDTVLLPAVLMLHGEEACVLLGWD
                     ESGENARLLFPETGQGTVVLGRAELAARYTGIAIFARPHFRFDKRTPQVGEIKLRHWF
                     WGAMADQWPVYRDVLAAALLINVMALTMPLFTMNVYDRVVPNRAIETLWVLAFGVALV
                     IVFDLVLRTLRGYFIDLASARIDMQLSAKIMERVLGVRMEARPAAVGSFASNLRSFES
                     VRDFITSATVTTFIDLPFALLFLLVIGIIAWPLIFPVLVAMILVTVYAYVLQHKMHEL
                     SETTYRAGALRNATLIESLTALETIKTQGAEGVMQSKWEKSVAFVARVNNQMRFLSAA
                     ATNGAMEVQQLVNVMVVIAGVYLISEGMLSMGGLIACTMLSSRAVAPLGQVVGLLMQY
                     HNAKVALTSLEQVMANPVERPNDANFVHRPELRGNIEFRDVAFNYPNTQVAALKSVNF
                     SIREGEKVVVIGRVGSGKTTLQKLLMGLYQTTGGAVSIDGVDVRQLDPADLRRNIGYV
                     PQDVTLFYGTMRDNISIGAPFADDAAILAAAEAGGLTEFVNRHPDGFEMMIGERGDSL
                     SGGQRQGVAIARAFLMDPPILLLDEPTSA"
     gene            complement(2063..3334)
                     /locus_tag="PLN02_02020"
     CDS             complement(2063..3334)
                     /locus_tag="PLN02_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOI51126.1"
                     /translation="MTGRNAFYAQSGGVTAVINATACGLIQEARRLRLQQPGSIGKVY
                     AGHDGILGALTEEMIDTDLESDADIARLRYTPGGAFGSSRYHLGPLATHRRHFERLIE
                     VFRAYDIGYVFINGGDGSMGAAWHLSQTLAELGYPLQVIGLPKTVDNDLVLTDTCPGF
                     GSVAKYVATSMREAGFDVASMARTSTRIFILEAMGRHAGWIAAAAGLASGHEGEPPHL
                     LLFPEVRFDAERFLARVEACVQRFGYCTIAAAEGLCDEGGCAIGALPQSELPSHTQAG
                     GVGRYIAELIHARLGYKYHLALADYLQRAARHLASGTDVEQAHALGVAAVDRAVAGGR
                     DIMLTLHRLADRPYRWEIGEVPLTAVANRERRMPAEFIAADGFHITDICRAYLQPLIA
                     GEDPPPFRDGLPDYVHLGNHRVPRRLQAFLP"
     gene            complement(3339..3992)
                     /gene="adk"
                     /locus_tag="PLN02_02025"
     CDS             complement(3339..3992)
                     /gene="adk"
                     /locus_tag="PLN02_02025"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOI51127.1"
                     /translation="MKLILLGAPGAGKGTQAKYICEKYGIPQISTGDMLRAQVKAGTP
                     LGLEAKKHMDAGGLVPDAVIIGMVKDRLQQDDCKNGYLFDGFPRTIPQAQAMKDAGVP
                     IEFVLEIDVPDSDIIERMAGRRAHLASGRTYHVKFNPPKVEGKDDVTGEDLVQRDDDK
                     EETVKKRLDVYHSQTKPLVEFYGKWAAEGDAAAPQVRKIAGVGSVDSITAAVFDALK"
     gene            complement(4078..4848)
                     /gene="kdsB"
                     /locus_tag="PLN02_02030"
     CDS             complement(4078..4848)
                     /gene="kdsB"
                     /locus_tag="PLN02_02030"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOI51128.1"
                     /translation="MTPAFKIVIPARYASSRLPAKPLLDLGGKPMVVRVAERARLAGA
                     EEVWVATDHPEVRAACEAHEVAALMTRADHATGTDRLAEVVAQRGWSNETIVVNVQGD
                     EPLIEPEVIVHTARQLAVSGADIATVAHPIAEPADFFNPNVVKVVCRADGDAAYFSRA
                     PVPYARDHFAREGGGANLPDGLPALRHVGLYAYRASFLKAYAGLAVAPTEQFEALEQL
                     RALWHGYRISVALVDAAPAPGVDTPEEAERMRKLFDRA"
     gene            complement(4848..5024)
                     /locus_tag="PLN02_02035"
     CDS             complement(4848..5024)
                     /locus_tag="PLN02_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOI51129.1"
                     /translation="MDARLLEILVCPICKGPLEFRKAAQELVCKPCKLAYPIRDDIPV
                     MLEDEARPLGADEA"
     gene            complement(5005..>5613)
                     /gene="lpxK"
                     /locus_tag="PLN02_02040"
     CDS             complement(5005..>5613)
                     /gene="lpxK"
                     /locus_tag="PLN02_02040"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOI51130.1"
                     /translation="AGRALLAAHPAVDLLLCDDGLQHYRLARDVEIAVFDRRGAGNGW
                     RLPLGPLREPLARLAEVDAVVGNDFAATELAGGRPAFAMTLRAGDFYRLDDPAVRCSA
                     DGLRGRPLHAVAGIGDPGRFFRTLAGLGLDCVPHPFPDHHAYAAGDLAFGDDAVLLMT
                     EKDAVKCAGLTAAETWVLPVDADLPPALTDLIVEKLRGRPIA"
BASE COUNT          951 a         1827 c         1907 g          928 t
ORIGIN      
        1 gcactggtcg gttcgtcgag cagcaggatc ggcgggtcca tcaggaaggc gcgggcgatc
       61 gccacgcctt ggcgctggcc gccggacaag gagtcaccgc gctcgccgat catcatctcg
      121 aaaccgtccg gatggcgatt gacgaattcc gtcaggccac ccgcttcggc cgccgccagg
      181 atcgccgcgt cgtcggcgaa gggcgcgccg atcgagatgt tgtcgcgcat ggtgccgtag
      241 aacagcgtca cgtcctgcgg cacgtaaccg atgttgcggc gcaggtcggc cggatcgagc
      301 tggcggacat cgacgccgtc gatgctgacc gcgccgccgg tcgtctggta gagccccatc
      361 aagagtttct gcaaggtcgt cttgccggaa ccgacgcggc cgatgacgac gaccttctcg
      421 ccctcgcgga tcgagaaatt gacgctcttc agcgctgcaa cctgggtatt cgggtagttg
      481 aaggcgacgt cgcggaactc gatgttgccg cgcagctccg ggcgatggac gaaattggca
      541 tcgttcggac gctcgaccgg gttggccatg acctgttcga gcgaggtcag cgccaccttg
      601 gcgttgtgat actgcatcag caggccaacc acctggccga gcggcgccac cgcccgcgac
      661 gacagcatgg tgcaggcgat caggccaccc atgctgagca tgccttcgct gatcagatag
      721 acgccggcga tgacgaccat cacgttgacc agttgctgca cttccatcgc gccgttggtc
      781 gccgccgccg agaggaaacg catctggttg ttgacgcggg cgacgaaggc caccgatttt
      841 tcccacttgc tctgcatgac gccttcggcg ccttgcgtct tgatcgtttc cagcgccgtc
      901 aggctttcga tcagcgtcgc gttgcgcaag gcgccggcgc ggtaggtggt ttccgaaagc
      961 tcgtgcatct tgtgctgcag cacgtaggcg tagaccgtca ccaggatcat cgccaccagc
     1021 accgggaaga tcagcggcca ggcaatgatg ccgatgacga gcaggaaaag cagtgcaaac
     1081 ggcagatcga tgaaggtggt caccgtcgcc gaggtgatga agtcgcgcac cgattcgaag
     1141 gaacgcaggt tggaagcgaa ggagccgacc gccgccggcc gcgcttccat gcgcacgccg
     1201 aggacgcgtt ccatgatctt ggccgacaac tgcatgtcga tgcgggcgct ggcgagatcg
     1261 atgaaatagc cgcgcagggt acgcagcacc agatcgaaga cgatcaccag cgcgacaccg
     1321 aaagccagca cccagagcgt ctcgatcgcc cggttgggca ccacccggtc atagacgttc
     1381 atcgtgaaca gcggcatggt cagcgccatc acgttgatca gcaaggcggc ggccagcacg
     1441 tcgcgataga ccggccactg atcggccatg gcgccccaga accagtggcg cagcttgatc
     1501 tcgccgacct gcggcgtgcg cttgtcgaaa cggaaatgcg gccgggcgaa gatggcgatc
     1561 ccggtgtagc gcgcggccag ttcggcccgc ccgaggacaa ccgtgccctg cccggtttcc
     1621 gggaacagca ggcgggcgtt ctcgccgctt tcgtcccaac cgagcaggac acaggcttct
     1681 tcgccgtgca gcatgaggac cgccggcaac agcaccgtat cgatccgctc cagcggccgc
     1741 cgcgacagtt tggcggagag gccggcacga gccgcggcac gcgggaacaa cgacggcgtg
     1801 aggacgcctt tttccagcgg tagcccggcg accaatgccg cccgggtgcc aggacgccca
     1861 tgaatacggg tcagttcaac caggcagtca agcagcggat cgtggtgcaa cagatccgcc
     1921 cgaactccgg ttttgaccgc ctcaacggaa atgctcatcc ctgttggtgc ccttcatgtt
     1981 atttgtatgg ttgccggatt ctacctttca gcgcagcggc aaatgcaaaa cattgcaaat
     2041 tgcgtcactc cacgagcatg cctcagggca ggaaagcctg caagcgccgg ggaacccggt
     2101 gattgccgag atgcacgtag tccggcaagc catcgcggaa aggcggcgga tcctcgcctg
     2161 cgatcagcgg ctgcagatac gcccggcaga tgtcggtgat atgaaagccg tcggcggcaa
     2221 tgaattcggc cggcatccgc cgctcgcggt tggccaccgc ggtcagcggc acttcgccga
     2281 tttcccagcg gtagggccgg tcggcgaggc gatgcagggt cagcatgatg tcgcgcccgc
     2341 cggccaccgc ccggtcgacc gcggcgacgc ccagggcatg cgcctgttcg acgtcggtgc
     2401 cggaggcgag atggcgcgcc gcccgctgca ggtaatcggc gagcgccaga tggtacttgt
     2461 agccgagccg ggcatggatc agctcggcga tgtagcggcc gacgccgccg gcctgggtat
     2521 ggctgggcag ctcgctctgc ggcagggcgc cgatcgcgca accgccctcg tcgcacaggc
     2581 cttcggcggc agcgatcgtg caatacccga aacgctggac gcaggcctcg acgcgggcga
     2641 ggaagcgctc ggcgtcgaaa cggacttcgg gaaacagcag caggtgcggc ggctcgcctt
     2701 cgtgcccgct ggccagcccg gcggcggcgg cgatccagcc ggcgtggcgg cccatcgctt
     2761 cgaggatgaa aatgcgggtc gaggtgcgcg ccatcgaggc gacgtcgaag ccggcctcgc
     2821 gcatcgaggt cgcaacgtac ttggcgaccg aaccgaaacc ggggcaggta tcggtcagca
     2881 ccaggtcgtt gtcgacggtt ttcggcaggc cgatcacctg cagcggatag ccgagctcgg
     2941 cgagcgtctg cgacaggtgc caggcggcgc ccatgctgcc atcgccaccg ttgatgaaga
     3001 cgtagccgat gtcgtaggcg cggaagacct cgatcagccg ttcgaaatgg cgccggtgcg
     3061 tggccagcgg cccgaggtgg tagcggctgg agccgaaggc gccgccgggc gtgtagcgca
     3121 ggcgggcaat gtcggcatcg ctttcgagat cggtatcgat catctcctcg gtcagcgcac
     3181 cgaggatgcc gtcgtgaccg gcgtacacct tgccgattga tccgggctgc tgcaaacgca
     3241 aacggcgtgc ttcctggatc aggccgcacg ccgtcgcgtt gatgacggcg gtaacgcctc
     3301 ccgactgcgc gtagaaggcg ttcctcccgg tcatcggctt acttcagggc gtcgaaaacc
     3361 gccgcggtga tgctgtccac gctgccgacc ccggcgatct tgcgcacctg cggcgctgcg
     3421 gcgtcgccct cggccgccca cttgccgtag aactcgacca gcggcttggt ctgcgagtga
     3481 tagacgtcga gccgcttctt gaccgtttct tccttgtcat cgtcgcgctg caccaggtct
     3541 tcgccggtga cgtcgtcctt gccctcgacc ttgggcgggt tgaacttgac gtggtaggta
     3601 cggcccgacg ccaggtgggc gcggcggccg gccatgcgct cgatgatgtc gctgtcggga
     3661 acatcgattt cgagcacgaa ctcgatcggc acgccggcgt ccttcatcgc ctgcgcctgc
     3721 gggatggtgc gcgggaagcc gtcgaacagg taaccgttct tgcagtcgtc ctgctgcagg
     3781 cggtccttga ccatgccgat gatcacggcg tccgggacca ggccgccggc atccatgtgc
     3841 ttcttggctt ccaggccgag cggggtgccg gccttgacct gggcgcgcag catgtcgccg
     3901 gtcgatatct gcggaatgcc gtacttttcg cagatgtact tggcctgcgt acccttgccg
     3961 gcgccgggtg cgcccaaaag aatcaatttc attgagaagt ccctctggtt acgtatgaaa
     4021 attcttgttg tcggctgttg tggcgccgtt ctctagcgcc cgaaattacc tgcttattca
     4081 ggctcggtca aacagtttgc gcatccgctc ggcctcttcg ggcgtatcga caccgggcgc
     4141 cggcgccgca tccaccaggg cgacgctgat ccggtagccg tgccacaggg cgcgtagttg
     4201 ttcgagcgcc tcgaactgct cggtcggcgc gacggcgagg ccagcgtagg ccttcaggaa
     4261 gctggcgcgg taggcgtaga ggccgacgtg gcgcagcgcc ggcaggccgt cgggcaggtt
     4321 ggcgccgccg ccctcgcggg cgaagtgatc gcgcgcatag ggcaccggcg cgcgcgagaa
     4381 ataggcggcg tcgccgtcgg cgcggcagac caccttgacc acgttgggat tgaagaaatc
     4441 ggccggctcg gcgatcggat gggcgacggt ggcgatatcg gcgccgctga cggcgagctg
     4501 gcgcgcggtg tggacgatga cttccggctc gatcagcggc tcgtcgccct gcacgttgac
     4561 aacgatggtt tcgttgctcc agccgcgctg ggccaccact tctgccaggc ggtcggtgcc
     4621 ggtggcgtgg tcggcgcgcg tcatcaacgc ggcgacttca tgcgcctcgc aggcggcccg
     4681 gacttccgga tgatcggtcg ccacccagac ttcttcggca ccggccaggc gcgcccgttc
     4741 ggcaacgcgg acgaccatcg gcttgccgcc gagatcgagc aacggtttgg ccggcaggcg
     4801 gctcgacgcg taacgcgccg gaatgacgat cttgaacgcc ggcgtcatca cgcttcgtcg
     4861 gcgcccagcg gccgtgcctc gtcctcgagc atgaccggaa tgtcgtcgcg gatcgggtag
     4921 gccagcttgc acggcttgca caccagttcc tgcgccgcct tgcggaattc gagggggccc
     4981 ttgcagatcg ggcagacgag tatttcaagc aatcgggcgt ccacggagtt tctccactat
     5041 caaatcggtc aatgccggcg gcagatcggc gtcgacgggc agaacccagg tctccgccgc
     5101 cgtcagtccg gcgcatttta ctgcatcctt ctcggtcatc agcagcaccg cgtcgtcgcc
     5161 gaaggcgagg tcgccggccg cgtaggcgtg atgatcgggg aaagggtggg gcacgcagtc
     5221 caggccgagg ccggccagcg tgcggaaaaa gcgtccggga tcgccgatcc ctgccaccgc
     5281 gtgcagcggc cggccgcgca agccgtcggc tgagcaacgg acggccggat cgtcgagacg
     5341 gtagaagtcg ccggcgcgca gcgtcatcgc gaacgccggc cggccgccgg ccagctcggt
     5401 cgcggcaaaa tcgttgccga ccacggcatc gacttcggcc aaccgcgcca gcggctcgcg
     5461 cagcggcccc agcggcaggc gccagccgtt gccggcgccg cgccggtcga agacggcgat
     5521 ctcgacgtcg cgcgccagcc ggtaatgctg caggccgtcg tcgcaaagca gcaggtcgac
     5581 cgccggatgc gccgccagca gcgcccggcc ggc
//
LOCUS       DAOSDX010000057         5556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5230_length_5556_cov_3.268678, whole genome shotgun sequence.
ACCESSION   DAOSDX010000057 DAOSDX010000000
VERSION     DAOSDX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_5556_cov_3.268678"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1146)
                     /gene="dmeF"
                     /locus_tag="PLN02_02045"
     CDS             complement(157..1146)
                     /gene="dmeF"
                     /locus_tag="PLN02_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HOI51131.1"
                     /translation="MNAHLDLSRWAQTHEYGAGNARAEAGTRWVLWLTLITMIVEIAA
                     GWWFNSMALLADGWHMSSHALAIGLASLAYAAARKYAEDPSFAFGTWKIEVLAGYTSA
                     LCLLGVAALMVWGAVERLIEPAPIAYGEAMLVAVLGLAVNLGSAAILLRAGGHDHHGH
                     DHGHSHDHGHDHHHGHDDLNLKAAYIHVLTDALTSVLAIAALAGGWFWGWNWLDPACA
                     MVGAVLVALWAKNLLRDTSRVLLDREMDHPVVEEIREVIGELPPGTQITDLHVWRVGN
                     GAYACAISLLTHDSALTPLAIRQALAIHEEIAHVTVEIHRCDECSPGAAFSGR"
     gene            complement(1143..1784)
                     /locus_tag="PLN02_02050"
     CDS             complement(1143..1784)
                     /locus_tag="PLN02_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOI51132.1"
                     /translation="MSELRAKLIAAARAMRPAGLNRGTAGNLSVRCGDGFLITPTGMP
                     YDALEADDVPLMAIDDSADGNFSGRRKPSSEWRFHRDLYASRPEVGAVLHAHSPFAVT
                     LACLRRAIPPFHYMIARFGGDTVRCADYALFGTPALSAAALAAMADRRGCLLANHGLL
                     VAGRDLDEAFALAVELEELCEQYWRACQLGEPVLLDAVDMAAVMNKFKTYGQQ"
     gene            complement(1781..2866)
                     /gene="mtnA"
                     /locus_tag="PLN02_02055"
     CDS             complement(1781..2866)
                     /gene="mtnA"
                     /locus_tag="PLN02_02055"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOI51133.1"
                     /translation="MNIHGTPTRTLRAATARRAIEIIDQTRLPHALHWVGVVTLDEAA
                     HAIRAMQVRGAPLIGATAAYGLAIALNDEAGDARLDDAIAILGATRPTAVNLHWALAR
                     MDKVLRPLPPAQRSNAAWAEAAAIAEEDVAQNAAIGRHGLELLRRLPRRDGRVLNVMT
                     HCNAGWLATVDWGTALAPVYAAHDAGLPVHVWVSETRPRNQGLLTAWELAQHGVPHTL
                     LADNAAGLLMRTGQVDAVIVGADRIAANGDVANKVGTYLKALACADNDIPFYVAAPRS
                     TLDFACADGAAIPIEERDGDEFRFVHGTDPRGTPAAVRQLPAGEAVANPAFDVTPARL
                     VAAIITERGACPASRAGLLGLYPEETA"
     gene            complement(2863..3255)
                     /locus_tag="PLN02_02060"
     CDS             complement(2863..3255)
                     /locus_tag="PLN02_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOI51134.1"
                     /translation="MTGWIWLETSVVGAVHEAQLTEHGGPIGIRDTGLLTSALARPVN
                     LAADDAQADAAALAAAYAFGIARNHPFIDGNKRTAFVCSELFLTLNGLCLMASDSDCV
                     GAMLALAAGDIGETEFAAWLRLHLLPAP"
     gene            complement(3252..3476)
                     /locus_tag="PLN02_02065"
     CDS             complement(3252..3476)
                     /locus_tag="PLN02_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOI51135.1"
                     /translation="MYALKLTQIGNSVGVVLPKELLAKLHVEKGDTVYATESPDGVRL
                     TPFDPAFEKQMEAAKKIMKARRNVLHELAK"
     gene            3591..4760
                     /locus_tag="PLN02_02070"
     CDS             3591..4760
                     /locus_tag="PLN02_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOI51136.1"
                     /translation="MQTSVHIDSRFPAMGTTIFTVMSRLAAETGAVNLAQGFPDFQAE
                     DALFDAMHRHLRAGRNQYAPMAGMPELRTAIAGKVATLYGAAYDSETEITVTAGATQA
                     IFTAIAAFVRPGDEVIVFAPVYDSYVPAIETVGGKAIYAHLRFPDYAPDWDEVRALIT
                     PRTRMIVVNSPHNPTGALLSAADLDALAGVVRGSDIVVLSDEVYEHILFDGAKHASLS
                     GHPELAARSIVVSSFGKTYHITGWKIGYVVGPAALMAEFRKVHQFNVFTVHGPSQLAI
                     ADYMQDASRHLGLAAFYQEKRDAFRQLLAATPFELLPCRGTYFQLARYERMSELADRD
                     FAEWLTREVGVAVIPVSVFQPDGRDDKVVRFCFAKREETLVAAAARLQRAFPGQS"
     gene            4845..5270
                     /gene="mscL"
                     /locus_tag="PLN02_02075"
     CDS             4845..5270
                     /gene="mscL"
                     /locus_tag="PLN02_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOI51137.1"
                     /translation="MGMLQEFKEFAVKGNAMDLAVGVIIGAAFGKIVDSVVGDLIMPL
                     VSMVVGKLDFSNLFVVLGDNPENLATLAELKKAGIPVFAYGSFFTILVNFVILAFIIF
                     MIVKQMNRLRKEDAAPAPTEPPPPEDVVLLREIRDALRK"
BASE COUNT          868 a         1732 c         2005 g          951 t
ORIGIN      
        1 tactttcttt tttccacaaa aaagaaagta cgcccgcgcg tcaggcgcgg aacccggcgt
       61 ttcagaagcc aaagccgcaa agccgcccgc cggtcaacgg cggaaaacag cgcttctact
      121 ggaacagaaa cccgctactt cgccgccgga tcggcattac cgcccggaga acgccgcgcc
      181 gggggagcac tcgtcgcagc ggtgtatctc caccgtgacg tgggcgattt cctcgtggat
      241 ggcgagggcc tggcggatgg cgagcggggt cagcgcgctg tcgtgggtga gcagcgagat
      301 ggcgcaggcg taggcgccgt tgccgacgcg ccagacgtgg aggtcggtga tctgcgtgcc
      361 gggcggcagt tcgccgatga cttcgcggat ttcctcgacc accgggtggt ccatctcgcg
      421 gtcgagcagg acgcggctgg tgtcgcgcag caggttcttc gcccacagcg cgacgagcac
      481 ggcgccgacc atggcgcagg cggggtcgag ccagttccag ccccaaaacc agccgccggc
      541 gagggcggcg atggcgagga ccgaggtcag cgcgtcggtg agaacgtgga tgtaggcggc
      601 cttgaggttg aggtcgtcgt ggccgtgatg atgatcgtgc ccgtggtcat ggctgtgccc
      661 atgatcatga ccgtggtggt cgtggccgcc ggcgcgcagc aggatcgccg cgctgccgag
      721 gttgacggcg aggccgagga cggcgaccag catggcttcg ccgtaggcga tcggcgccgg
      781 ttcgatcagg cgttcgacgg cgccccagac catcagcgcg gcgacgccga gcaggcagag
      841 ggcgctggtg tagccggcga ggacttcgat cttccaggtg ccgaaggcga agctggggtc
      901 ttcggcgtac ttgcgggcgg cggcgtaggc gaggctggcg aggccgatgg ccagcgcgtg
      961 cgagctcatg tgccagccgt cggcgagcag cgccatcgag ttgaaccacc agccggcggc
     1021 gatctcgacg atcatcgtga tcagggtcag ccacagcacc cagcgggtgc cggcttcggc
     1081 gcgtgcgttg ccggcgccgt attcatgcgt ttgtgcccag cgcgagaggt cgaggtgtgc
     1141 gttcattgtt gtccgtaggt tttgaatttg ttcatcactg ctgccatgtc gaccgcgtcg
     1201 agcagtaccg gttcgccgag ctggcaggcg cgccagtatt gttcgcagag ttcctcgagt
     1261 tcgacggcga gggcaaaggc ttcgtcgagg tcgcggccgg cgacgagcag gccgtggttg
     1321 gcgagcaggc agccgcggcg gtcggccatg gcggcaagcg ccgccgccga cagcgccggc
     1381 gtgccgaaca gtgcatagtc ggcgcagcgc acggtatcgc cgccgaagcg ggcgatcatg
     1441 tagtggaagg gcgggatggc gcggcgcagg caggccagcg tgacggcgaa cggcgagtgg
     1501 gcgtgcagga cggcgccgac ttccgggcgg ctggcgtaga ggtcgcggtg aaagcgccat
     1561 tccgacgacg gcttgcggcg gccgctgaag ttgccgtcgg cgctgtcgtc gattgccatc
     1621 aacgggacgt cgtcggcttc cagggcgtcg tagggcatgc cggtcggcgt gatcaggaag
     1681 ccgtcgccgc agcgcacgct gaggttgccg gcggtgccgc ggttgaggcc ggccgggcgc
     1741 atggcgcggg cggcggcaat cagcttggcg cgcagttcgc tcatgccgtt tcttccgggt
     1801 agaggccgag caggccggca cggctggccg ggcaggcgcc gcgttcggtg atgatggcgg
     1861 cgaccaggcg ggccggggtg acgtcgaaag ccgggttggc gacggcttcg ccggccggaa
     1921 gctggcgcac ggcggccggc gtgccgcgcg ggtcggtgcc gtgcacgaag cggaactcgt
     1981 cgccgtcgcg ttcctcgatc gggatcgcgg cgccgtcggc gcaggcgaag tcgagcgtcg
     2041 agcgcggcgc ggcgacgtag aagggaatgt cgttgtcggc gcaggccagc gccttcaggt
     2101 aggtgccgac cttgttggcg acgtcgccgt tggcggcgat gcggtcggcg ccgacgatca
     2161 ccgcatcgac ctggccggta cgcatcagca ggccggccgc gttgtcggcg agcagggtat
     2221 gcggcacgcc gtgctgggcg agttcccagg cggtgagcag gccctggttg cgcggccggg
     2281 tttcggaaac ccagacatgc accggcaggc cggcgtcgtg cgcggcatag accggggcga
     2341 gcgcggtgcc ccaatcgacg gtggcgagcc agccggcgtt gcagtgggtc atcacgttga
     2401 gcacgcggcc gtcgcggcgc ggcaggcggc gcagcagctc cagcccgtgc cggccgatcg
     2461 ccgcgttctg cgccacgtcc tcctcggcga tcgccgccgc ttccgcccag gcggcgttgc
     2521 tgcgttgtgc cggcggcagg gggcgcagga ccttgtccat gcgggccagt gcccagtgca
     2581 ggttgaccgc ggtcggccgg gtggcgccga ggatggcaat cgcatcgtcc agacgggcgt
     2641 cgccggcctc gtcgttgagc gcgatggcca ggccgtaggc ggcggtggcg ccgatcagcg
     2701 gcgcgccgcg cacctgcatg gcgcggatgg cgtgcgccgc ttcgtccagc gtgacgacgc
     2761 cgacccagtg cagcgcgtgc ggcaggcggg tctggtcgat gatttcgatg gcgcgccgag
     2821 cggtggcggc acgcagggtg cgggtggggg tgccgtggat gttcatgggg cggggagaag
     2881 gtggaggcgc agccaggcgg cgaactcggt ttcgccgatg tcgccggcag cgagggcgag
     2941 catggcgccg acacagtcgc tgtcggaagc catcaggcaa aggccgttga gggtgaggaa
     3001 gagttcgctg cagacaaagg cggtgcgttt gttgccgtca atgaagggat ggttgcgggc
     3061 gatgccgaaa gcgtaggcgg cagcgagcgc cgccgcgtcg gcttgcgcgt cgtcggcggc
     3121 caggttgacc gggcgggcca gcgccgaggt cagcaggccg gtgtcgcgga tgccgatcgg
     3181 accgccgtgc tcggtcagct gcgcttcgtg gaccgccccg accaccgatg tttcgagcca
     3241 gatccagccg gtcatttggc cagttcgtgc agcacgttgc ggcgtgcctt catgattttt
     3301 ttcgccgctt ccatctgctt ctcgaaggcc gggtcgaaag gcgtcagacg cacgccgtcg
     3361 ggcgattccg tggcatagac ggtgtcgccc ttctcgacat gcagtttggc cagcaactcc
     3421 ttgggcagca cgacgccgac cgaattgccg atctgggtca gtttgagcgc gtacatgatg
     3481 gcctccggct gttataacgg atgtaatact acgcgcaatt tcggcgcccg gcttgtccgc
     3541 cgtccggcct tggcgcaaga ttgggcccct ttccgtccgc catgcttgcg atgcaaactt
     3601 ccgtccacat cgactcccgc ttccccgcca tggggacgac gatcttcacc gtgatgtcgc
     3661 ggctcgccgc cgagaccggc gccgtcaatc tggcgcaggg ctttcccgat tttcaggccg
     3721 aagacgcgct gttcgacgcc atgcatcgtc acctgcgggc cggccgcaac cagtacgcgc
     3781 cgatggccgg catgccggag ctgcgcacgg cgattgccgg caaggtggcg acgctgtacg
     3841 gcgcggctta cgacagcgaa acggaaatca ccgtcacggc cggggcgacg caggcgatct
     3901 tcaccgcgat tgccgccttc gtccggccgg gcgacgaggt catcgtcttc gcgccggtgt
     3961 acgacagcta tgtgccggcc atcgaaacgg tcggcggcaa ggcaatctac gcccacctgc
     4021 gttttcccga ttacgcgccg gactgggacg aggtgcgggc gctgatcacg ccgcggacgc
     4081 ggatgatcgt cgtcaactcg ccgcacaatc cgaccggcgc gctgctctcc gcggccgatc
     4141 tcgacgcgct ggcgggcgtc gtccggggga gcgacatcgt cgtcctgtcc gacgaggtct
     4201 acgagcacat cctgttcgac ggcgcgaagc acgccagcct gtccggtcat ccggaactgg
     4261 cggcacgcag catcgtcgtt tccagcttcg gcaagaccta tcacatcacc ggctggaaga
     4321 tcggctacgt cgtcggcccg gcggcgttga tggccgaatt ccgcaaggtg caccagttca
     4381 acgtgttcac cgtgcacggg ccgtcgcaac tggcgatcgc cgactacatg caggacgcct
     4441 cgcgccatct tggcctggcc gccttctacc aggagaagcg cgatgccttc cggcaactgc
     4501 tggcggcgac gcccttcgag ctgctgccct gtcgtggcac ctactttcaa ctggcgcgtt
     4561 acgagcggat gtcggagctg gcagaccgcg atttcgccga atggctgacg cgcgaggtcg
     4621 gcgtcgcggt gatcccggtt tccgtcttcc agccggacgg gcgcgacgac aaggtggtgc
     4681 gattctgctt cgccaagcgt gaggaaaccc tggtcgctgc agcggcgagg ctgcagcggg
     4741 cgtttcccgg tcaatcttga aatattgcaa atcgccttga ccgcgcttgg cgcgtcgccg
     4801 aataatccgc ccggtaaaac tacttcaccg gaaaggggaa aagcatgggc atgttgcagg
     4861 agttcaagga atttgccgtc aagggcaatg cgatggatct ggcggtcggc gtcatcatcg
     4921 gtgccgcctt tggcaagatc gtcgattcgg tggtcggcga cctgatcatg ccgctggtca
     4981 gcatggtcgt cggcaagctc gatttctcca acctgttcgt cgtcctcggc gacaacccgg
     5041 agaacctggc caccctggct gaactgaaaa aggccggcat cccggtcttc gcctacggtt
     5101 ccttcttcac catcctggtc aatttcgtca tcctggcctt catcatcttc atgatcgtca
     5161 agcagatgaa ccgcttgcgc aaggaagacg cggcgccggc gccgaccgaa ccgccgccgc
     5221 cggaggatgt cgtcctgttg cgggaaatca gggacgcgtt gcgcaagtag gcgctcacaa
     5281 tttgtgtttc tgggccgcca tcagggcggc ctttttgcgt cgtggggctg ctggctggtg
     5341 caagcaatcg taagcaagtg ttaaatcggt ccactgaagg cggcagcagc cgttaatgtg
     5401 atcatgaaaa cacaaacgga gagaagatca tgaagcgcag gctcatcctc atcgcgctgc
     5461 tcggcagcct gctcggcgct tgcgccgtgg tgccggccga tccttattac gacgacaacg
     5521 tgatcatcac ggcgccgccg ccaccgccgc gcgtcg
//
LOCUS       DAOSDX010000058         5518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5280_length_5518_cov_3.083288, whole genome shotgun sequence.
ACCESSION   DAOSDX010000058 DAOSDX010000000
VERSION     DAOSDX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_5518_cov_3.083288"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLN02_02080"
     CDS             complement(<1..932)
                     /locus_tag="PLN02_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOI51138.1"
                     /translation="MKIAFRPKLLDCLPTYDRAQFGKDLGAGITVGVLALPLAMAFAI
                     ASGVSPTAGIWTAIVAGLIISLLGGSRVQIGGPTGAFIPIIYGIVAAHGFANLLGATM
                     LAGVMLLAMGLTRMGQLIRFIPVTVVIGFTNGIAVVIFLAQIKDFFGLTIASLPAEFF
                     ARIKVLAAHAHTVDLPTLALSIACFVFLLSYNRLAQRFAPLRRAPGPLAVLVVGTVAA
                     WALDLPVETIGSRFNGIPQELPAFGLPAMSIHDFGKLISPAITIALLGAIESLLSARV
                     ADSQIDDRHDPNQELVAQGLANIAAPLCGGFAATG"
     gene            1043..1234
                     /locus_tag="PLN02_02085"
     CDS             1043..1234
                     /locus_tag="PLN02_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOI51139.1"
                     /translation="MEKRTARLTLLIDPRKKQIFEEICAAQDLTPSQVVRRLIRQYII
                     DNAGERELPDWLRASVRGD"
     gene            complement(1231..1485)
                     /locus_tag="PLN02_02090"
     CDS             complement(1231..1485)
                     /locus_tag="PLN02_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51140.1"
                     /translation="MKAQPSPLRSRLLGYLLADLFGFLCLALGVAWFAGGTGVILDSF
                     PRSTAEALASAGGGLVVMVWAIGRILRELGRLAPPRADGD"
     gene            complement(1883..3964)
                     /gene="metG"
                     /locus_tag="PLN02_02095"
     CDS             complement(1883..3964)
                     /gene="metG"
                     /locus_tag="PLN02_02095"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOI51141.1"
                     /translation="MSRKILVTSALPYANGAIHLGHLVEYIQTDIWVRFQKMSGNECW
                     YVCADDTHGTPIMLRAEKEGITPEQLIARVHGEHSRDFAGFLVGFDNYYSTHSPETRD
                     CANDIYGKLKAAGLIEVRSIEQYYDPVKQMFLPDRFIKGECPKCHAKDQYGDNCEVCG
                     AADAPTDLIEPFSAVSGAKPELRHSDHYFFKLSDPRCEAFLREYTSSAVLQAEAANKM
                     QEWLGAPGDNKLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPIGYMGSFRNLCAQKG
                     LDFDEFFKKDSTTELYHFIGKDILYFHALFWPAELQHAGYRTPTKIFAHGFLTVDGAK
                     MSKSRGTFITAESFLNSGMNPEWLRYYFAAKLSNTMEDIDLSFDDFVARVNSDLVGKY
                     VNIASRAAGFIAKRFNGQLAPADDSLPAIRAIREAAPRVAELFEAREFSKAIREIMAL
                     TDGANQYVDSVKPWELAKQEGKDSELHAACSNALNLFRLLTVLLKPVLPATAAKVEAF
                     MNLAPLSWADAGTLLAGGHAINAYSHLMTRVDAKQIEALIEANKQSLAPAAAEAPAKP
                     AAKAAEKKEVPASTDGLCNIDDFMKVDLRIAKIVNAEHVDGADKLVRLTLDIGEEKTR
                     NVFAGIKAAYDPAQLVGRLTVMVANLAPRKMKFGLSEGMVLAASDLDGKTPGLYLLAP
                     DSGAQPGMRVK"
     gene            4135..4818
                     /locus_tag="PLN02_02100"
     CDS             4135..4818
                     /locus_tag="PLN02_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI51142.1"
                     /translation="MNFAVIEDSRSQAEVLKALLKSEGYQVEVFADGQSFLDVLATRS
                     FDFYVVDWVLPDIGGDVLVGKIRERYGWDVPIVVCTARTEEEHASDILRLGADDYVPK
                     PVRYMEFLARVDALLRRRRAKQPDLLRVGNIEVDIEGRRVRLAGQEVDLTQREFDLAA
                     VFLKNIGRVLPREELLAAVWARETDVDTRTVDTHASRLRKKLGLSGESGLMLTSVYGQ
                     GYRLDTVSG"
     gene            4894..>5518
                     /locus_tag="PLN02_02105"
     CDS             4894..>5518
                     /locus_tag="PLN02_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOI51143.1"
                     /translation="MSLTEQQVRNVLAETIDPNTGKSYLAGKCLKNLRLDADGVAFDI
                     ELGYPANSQHEIIRQQIVDALAALPGAGRINVRVSSNIVAHAVQLGVKLLPGVRNIIA
                     VASGKGGVGKSTTAVNLALALAQEGARVGLLDADIYGPSQPQMLGLAGRQPESLDGKT
                     MEPLEAYGVQAMSIGFMVDVETPMVWRGPMVAQALDQLLNETRWRDVD"
BASE COUNT          982 a         1585 c         1919 g         1032 t
ORIGIN      
        1 ccggtcgccg cgaagccgcc gcagagcggg gcggcgatgt tggccagccc ctgggcgacg
       61 agttcctggt tggggtcgtg gcggtcgtcg atctggctgt cggcgacgcg cgccgagagc
      121 agcgattcga tggcgccgag cagggcgatg gtgatcgccg gcgagatcag cttgccgaag
      181 tcgtggatcg acatggcggg caaaccgaaa gccggcagtt cctgcggaat gccgttgaag
      241 cggctgccga tggtttcgac cggcagatcg agtgcccagg cggcgacggt gccgacgacc
      301 agcacggcga gcgggccggg cgcccggcgc agcggcgcga agcgttgcgc cagccggttg
      361 taggaaagca ggaagacaaa gcaggcgatc gacaatgcca gcgtcggcag gtctacggta
      421 tgcgcgtgtg ccgccagcac cttgatccgg gcgaagaatt cggccggcag gctggcgatg
      481 gtgaggccga agaaatcctt gatctgggcg aggaaaatga cgacggcaat gccgttggtg
      541 aagccgatga cgacggtcac cgggatgaag cggatcagct ggcccatgcg cgtcaggccc
      601 atggccagca gcatgacgcc ggcgagcatg gtggcgccga gcaggttggc gaagccgtgg
      661 gcagcgacga tgccgtagat gatggggatg aaggcgccgg tcgggccgcc gatctgcacc
      721 cgcgaaccgc cgagcagcga aatgatcagg ccggcgacga tggcggtcca gatgccggcg
      781 gtcggcgaca ccccggaggc aatggcgaag gccatcgcca acggcagcgc cagaacgccg
      841 acagtgatcc cggcgccgag gtccttgccg aactgggccc ggtcgtaagt cggcaggcaa
      901 tccagcagct tgggacggaa ggcgatcttc atcatgacgc gcactcgggg agggaagcgt
      961 tgcattatat gagtatgcta ttaatcgtca atgttaatcg tatgttaata tcacgacgaa
     1021 ggaatacgac tagagaagac tcatggagaa acgcactgcc cgcctgacgc tgttgatcga
     1081 cccgaggaag aagcagatct tcgaggaaat ctgtgccgcg caggatctga cgccgtcgca
     1141 ggtcgtgcgc cggctgatcc gtcagtacat catcgacaat gccggcgaac gcgaattgcc
     1201 ggactggctg cgcgcttcgg tacgcggcga ttaatcgcca tccgcgcggg gcggcgccag
     1261 gcgtccgagt tcgcgcagga tgcggccgat cgcccagacc atgacgacca gcccgccgcc
     1321 agcgctggct agcgcttccg cggtgctgcg cggaaaactg tcgaggatca cgcctgtgcc
     1381 gccagcgaac caggcgacgc cgagggcgag gcaaaggaaa ccgaacaggt cggccagcag
     1441 gtagccgagc aggcgggagc gaagggggct gggctgggct ttcattgcgc cattctgaca
     1501 aaaaaatgcg tttcgggctt gacaagcgac tttgtcaagc ttggttatgc acagcgacga
     1561 ttgtttgtcg cggcccgcct tgctatctcg ctgatgaatt tgacagattc gcctggtggg
     1621 tgattagtcg cattgaatca gtgacttacg aatttgcgca agttttgcgc agggtaagga
     1681 aaagccttgt ggggcggtca cttagcgata tatcccgcaa ataaccaaca gacttatcca
     1741 caggttttgt ggatagatcg aaaacctcta ggccggggtg gctattgacc gccaaactta
     1801 aagtaacgct ttaagagttg ggcgaaaatg ctgcgctgca aaagaattgg gccgctattg
     1861 cggcccgggt ttttcttgcg tcctacttga cccgcatgcc aggttgcgcc ccgctgtcgg
     1921 gcgccagcag gtagagcccc ggcgtcttgc cgtcgaggtc ggaagcggcg agcaccatgc
     1981 cctcggagag gccgaacttc atcttgcgcg gcgccaggtt ggcgaccatc acggtcagcc
     2041 ggccgaccag ttgcgccgga tcgtaggccg ccttgatgcc ggcgaagacg ttgcgcgtct
     2101 tctcctcgcc gatgtccagc gtcaggcgca ccagcttgtc ggcgccgtca acgtgctcgg
     2161 cattgacgat cttggcgatg cgcaggtcga ccttcatgaa gtcgtcgatg ttgcacaggc
     2221 cgtcggtgct ggccgggact tccttctttt cggctgcctt ggcggcgggc ttggccgggg
     2281 cttcggcggc cgccggggcc agcgactgct tgttggcttc gatcagtgcc tcgatctgct
     2341 tggcgtcgac acgcgtcatc aggtggctgt aggcgttgat cgcgtggccg ccggcgagca
     2401 gtgtgccggc gtcggcccag ctcagcggcg ccaggttcat gaaagcctcg accttggctg
     2461 cggtagccgg cagcaccggc ttgaggagta cggtgagcag gcggaacagg ttgagcgcgt
     2521 tcgagcaggc ggcgtgcagt tcgctgtcct tgccttcctg cttggccagt tcccacggct
     2581 tgacgctgtc gacatactgg ttggcgccgt cggtcagggc catgatctcg cggatcgcct
     2641 tgctgaattc gcgcgcttcg aacagttcgg caacacgcgg tgctgcttcg cggatggcgc
     2701 ggatcgccgg caggctatcg tcggccggcg ccagttggcc gttgaagcgc ttggcgatga
     2761 aaccggcggc gcggctggcg atgttgacgt acttgccgac caggtcggaa ttcacgcggg
     2821 cgacgaagtc gtcgaagctg aggtcgatgt cttccatcgt gttcgacagc ttggcggcga
     2881 agtagtagcg cagccattcc gggttcatgc cgctgttgag gaagctctcg gcggtgatga
     2941 aggtgccgcg cgatttcgac atcttggcgc cgtcgacggt gaggaagccg tgcgcgaaga
     3001 tcttggtcgg cgtgcggtag ccggcgtgct gcagttcggc cggccagaac agcgcgtgga
     3061 agtagaggat gtccttgccg atgaagtggt agagctcggt cgtcgagtcc ttcttgaaga
     3121 actcgtcgaa atcgaggccc ttctgcgcgc acaggttgcg gaacgacccc atgtagccga
     3181 tcggtgcgtc gagccagacg tagaagtact tgcccggcgc gtccggtatc tcgaaaccga
     3241 agtaaggcgc gtcgcgcgag atgtcccagt cggacagctt gttgtcgccg ggggcgccca
     3301 gccattcctg catcttgttg gcggcttcgg cctggagcac ggcgctactg gtgtattcgc
     3361 gcaggaaggc ttcgcagcgc gggtcggaga gcttgaagaa gtagtggtcg gagtggcgca
     3421 gttccggctt ggcgccggag acggccgaga acggctcgat caggtcggtc ggcgcgtcgg
     3481 cggcgccgca gacctcgcag ttgtcgccgt actggtcctt ggcgtggcac ttcgggcatt
     3541 cgcccttgat gaagcggtcg ggcaggaaca tctgcttgac cgggtcgtag tactgctcga
     3601 tcgaacgcac ctcgatcagg ccggcggcct tgagcttgcc gtagatgtca ttggcgcagt
     3661 cgcgggtctc cggcgagtgg gtgctgtaat agttgtcgaa gccgaccagg aagccggcga
     3721 agtcgcgcga gtgctcacca tggacgcggg cgatcagctg ttccggcgtg atcccctctt
     3781 tttcggcgcg caacatgatc ggcgtgccgt gcgtgtcgtc ggcgcagacg taccagcatt
     3841 cgttgccgga catcttctgg aagcgcaccc agatgtcggt ctggatgtat tcgacgaggt
     3901 ggccgaggtg gatggcgccg ttggcgtagg gcagggcgga ggtgacgagg atcttgcgcg
     3961 acatggggcg acggagttgg gcaaaacccg gattctaccg cgctctggcc caaagccgag
     4021 ggagggcttg ttgcggcgcg ggagaaagct ggatgtcgca agcgtggatt gatacattta
     4081 tcagttgatc cggcggccag tgctgggcag aattcgtcct atccaggagg gattatgaat
     4141 tttgccgtta tcgaggacag ccgtagtcag gccgaagtgc tgaaggcttt gttgaagagc
     4201 gagggctatc aggtcgaggt gtttgccgac gggcagtcct ttctcgacgt gctggccacg
     4261 cgcagcttcg atttctacgt cgtggactgg gttctccccg atatcggcgg cgacgtgctg
     4321 gtcggcaaga ttcgcgagcg ttacggctgg gatgtgccga tcgtcgtttg taccgcgcgc
     4381 acggaggaag agcacgcctc cgacatcctg cgcctgggcg ccgacgacta tgtgccgaag
     4441 ccggtccgct acatggaatt cctcgcccgc gtcgatgcgc tattgcgtcg ccgccgggcc
     4501 aaacagccgg atctgctgcg cgtcggcaac atcgaagtcg atatcgaggg ccggcgggtt
     4561 cgtctggcgg gccaggaagt tgacctgacg cagcgcgagt tcgacctcgc cgccgtcttc
     4621 ctgaagaaca tcggccgggt gctgccgcgt gaggaactgc tggccgccgt ctgggcgcgc
     4681 gagaccgacg tcgatacgcg taccgtcgat acccacgcca gccgcttgcg caagaagctc
     4741 ggcctgtccg gcgaaagcgg cctgatgctg acctcggtct acggccaggg ttatcgcctg
     4801 gataccgtct ccggctgatt tgcggcgcgg ctttgggcta tactctacga aatcagtcgg
     4861 gtattgtccg acgccagccc aagcggagtg tccatgagcc ttaccgaaca gcaggtcagg
     4921 aatgtcctgg ccgaaaccat cgatcccaat accgggaaga gttatcttgc cggcaagtgc
     4981 ctgaagaatc tgcgccttga cgccgacggg gtcgccttcg acatcgaact cggctacccg
     5041 gcgaacagcc agcacgagat catccgccag cagatcgtcg atgcgctggc cgcgttgccg
     5101 ggcgccggtc ggatcaacgt ccgcgtctcc agcaacatcg tcgcccacgc cgtccagctc
     5161 ggcgtcaagc tgctgcctgg ggtgcgcaac atcatcgccg tcgcctccgg caagggcggt
     5221 gtcggcaaga gtacgacggc ggtcaacctg gcgttggcgc tggctcagga aggcgcccgg
     5281 gtcggcttgc ttgacgccga tatctacggc ccgtcgcagc cgcagatgct gggtctggcc
     5341 ggccgtcagc cggaatcgct cgacggcaag acgatggagc cgctcgaggc ttacggcgtg
     5401 caggcgatgt cgatcggttt catggtcgat gtcgagacgc cgatggtctg gcgcgggccg
     5461 atggtggcgc aggcacttga ccaattgctc aacgagacgc gctggcggga cgtcgatt
//
LOCUS       DAOSDX010000059         5454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5351_length_5454_cov_3.280793, whole genome shotgun sequence.
ACCESSION   DAOSDX010000059 DAOSDX010000000
VERSION     DAOSDX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5351_length_5454_cov_3.280793"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..2430
                     /locus_tag="PLN02_02110"
     CDS             10..2430
                     /locus_tag="PLN02_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HOI51144.1"
                     /translation="MTPVAQALLVVFLGAAAGAGLLPARAHAAEAGATVNFDIPAGSL
                     DQALSRFGRQAGIQIAVNAELTRGRQSTGLSGKHGIAEGLRRLLAGSGLEAVRDSSGE
                     YTLRRLPSTATGETTLAPMTVTAGGLGGTTENSGSYTTGAMSTATKLSLSPRETPQSV
                     SVLSRRQIEEQNLLSLDDAVNNVTGLVMQKGYYSGESGSFFARGFPVANLLIDGLPTS
                     AGANGTFNADNDALDIYDRVEVVRGATGLTTGAGTPSAAINLVRKRPTAENQASVTLS
                     AGSWQNLRSTADVSGPLNAAGTLRGRAVATAQDSKQFYDTAHDRNQQLYAIVEADLTP
                     ATVATLGFHYRNVDNDGFVPGHPTNPDGSFLAGLSRSANTANDFDYWQQTDKSVFAEL
                     SHKFANGWQLKAAALWKQPEQDMMFSGLYRNGGVLRQSTQRYRLDNRQDSYDVTANGP
                     FTLLGRSHELMFGASYREFDNKNWGGWSSYAWTAAGPAVDPYHWDSSAVAQPAIDMSL
                     WNWAYTARQKGVYAATRLNLADPLKLILGARINWYENDNHSNGTRFAINREVTPYAGL
                     VFDLDDSHSLYASWTEIFEPQSNRDSNGQLLAPITGTNHELGIKGEYFGGRLNASLAT
                     FVVRQQNRAVDDLAGPNPCPGSSWGYCKRAAGEVESKGVEFEVGGALTPSWQVMAGYT
                     YVAAEFTKDADRANIGQSYDSDLPRHQLKLSTSYRLPGELSRWRIGGNVYAQNRTKSS
                     DDANIQQAGYAIVGLNGSYQLDARTEIRLNINNLFDKHYYQGLGWTTGGNVFGAPRNF
                     VVTARYSF"
     gene            2896..3186
                     /locus_tag="PLN02_02115"
     CDS             2896..3186
                     /locus_tag="PLN02_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51145.1"
                     /translation="MHTNKDVLDRFQSLYEGLIAHDGFGEMRLEFRILKRGQKEVVIH
                     CGKQYRFVVDYRPNNPLAWEVRREPATAESPRPTPERRRRQEAIAFPDRRQR"
     gene            3337..4299
                     /locus_tag="PLN02_02120"
     CDS             3337..4299
                     /locus_tag="PLN02_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI51146.1"
                     /translation="MSNMLSRLMGKKDAEIQAIVANVDAAAAPRGLRRKFEKIRSKKQ
                     GGFTLLELLVVVAILAAIAGTATIMLHDTDKKAFAAAHVAMMDELSKGILSFQQLNAG
                     KYPNAWDSLMTNATGVLDATATPSILLNEGLADNLAQHVLTTEEFNALEAVGITQLRV
                     LNEAATGIPDQGTLTCANTEAGIRALIESKANNVTSQNIFRSPASNGCGAAATIADGN
                     ALLMWDQAQNERVNAKATDVLIAFGVGPDSTLFQSANYGALTNTPIYRHVEGNEYNRF
                     VALFNVTSDSTKPTNGKATFQAIVDGAGDTKDEELGEFDNVRPT"
     gene            4378..5301
                     /locus_tag="PLN02_02125"
     CDS             4378..5301
                     /locus_tag="PLN02_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI51147.1"
                     /translation="MTLSKAGELRDCGFTLIELLVVCSLLAALSYTAWGAYTGVQERA
                     EDDIARAEMLRLADALKRFRADTGYYPGQGPFALAAPGTVETASGDGYTCDPVGGVRR
                     SWAAPALDADKDAWFASPANLALLFAAPALCANHPLAYLERWQPASGRGWHGPYLDIS
                     ARAWVDHGNDFNADRNCALNGAPDGDGNPVAGSKIVDIPAFGNGPRFSAVGPAWEPCA
                     EQAHSTPSATCMLGWRSVPRQAAGYAAMRHELPAHARPFVVFGLADNDAPRLVYFGRD
                     GRYGGRNAADPCLPNLPDPDGADDQVLCLNR"
BASE COUNT         1028 a         2028 c         1604 g          794 t
ORIGIN      
        1 cgccacccgc tgacgcccgt cgcgcaagcg ctgctcgtcg tcttcctcgg cgctgccgcc
       61 ggcgccggcc tgctgcccgc ccgggcccac gccgccgaag ccggcgccac ggtcaatttc
      121 gacatccccg ccggcagcct cgaccaggcc ctgagccgct tcggccgcca ggccggcatc
      181 cagatcgcgg tcaatgccga actgacccgc ggccggcaaa gcacgggcct gagcggcaag
      241 cacggcatcg ccgagggcct gcgccgactg ctcgccggca gcggcctgga agcggtacgc
      301 gacagcagcg gcgagtacac cttgcgtcgt ttgccatcca ctgcaaccgg tgaaaccacg
      361 ctggcgccaa tgacggtgac cgccggcggc ctcggcggga ccaccgaaaa cagcggctcc
      421 tacaccaccg gcgcgatgag cacggcgacc aaactgagcc tgtcgccgcg cgagacgccg
      481 caatcggtca gcgtcctcag ccggcgccag atcgaggagc agaatctgct cagcctcgac
      541 gacgcggtca acaacgtcac cggcctggtc atgcagaagg gttactacag cggcgaatcg
      601 ggcagcttct tcgcccgcgg ctttcccgtc gccaacctgc tgatcgacgg cctgccgacc
      661 agcgccggcg ccaacggcac cttcaatgcc gacaacgacg cgctcgacat ctacgaccgg
      721 gtcgaggtgg tgcgcggcgc caccgggctg accaccggcg ccggcacgcc ctcggcggcg
      781 atcaacctgg tgcgcaagcg gccgaccgcg gaaaaccagg ccagcgtcac gctgagcgcc
      841 ggcagctggc agaacttgcg cagtaccgcc gacgtcagcg gcccgctcaa cgccgccggc
      901 acgctgcgcg gccgggccgt ggccacggcg caggacagca agcagttcta cgacaccgcc
      961 cacgaccgca accagcagct gtacgccatc gtcgaggccg acctgacgcc ggcgacggtg
     1021 gcgacgctgg gcttccacta ccgcaacgtc gacaacgacg gcttcgtccc cggccatccg
     1081 accaatccgg acggcagctt ccttgccggc ctgagccgtt cggccaacac cgccaacgat
     1141 ttcgactact ggcagcagac cgacaagagc gtcttcgccg agctcagcca caagttcgcc
     1201 aacggctggc agctcaaggc cgccgccctg tggaaacagc cggaacagga catgatgttc
     1261 tccggcctct accgcaacgg cggcgtgctc cgccagagca cccagcgcta ccggctcgac
     1321 aaccgccagg acagctacga cgtcaccgcc aacggcccgt tcaccctgct cgggcgcagc
     1381 cacgagctga tgttcggcgc cagctaccgc gaattcgaca acaagaactg gggcggctgg
     1441 tcgagctacg cctggaccgc cgccggaccg gcggtcgatc cctaccactg ggattcgtcc
     1501 gcggtcgccc agccagcgat cgacatgagc ctctggaact gggcttacac ggcccgccag
     1561 aaaggcgtct atgccgccac ccgcctcaat ctcgccgatc cgctcaagct catcctcggc
     1621 gcccggatca actggtacga gaacgataac cacagcaacg gtacgcgctt cgccatcaac
     1681 cgcgaagtca cgccctacgc cggcctggtt ttcgacctcg acgacagcca ctcgctctac
     1741 gccagctgga ccgagatctt cgagccgcag agcaaccgcg acagcaacgg ccagctgctg
     1801 gcgccgatca ccggcaccaa ccacgaactc gggatcaagg gcgaatactt cggcggccgc
     1861 ttgaacgcca gcctggcgac cttcgtcgtc cgccagcaga accgcgccgt ggacgatctc
     1921 gccggcccca atccctgccc cggcagcagc tggggctact gcaagcgggc cgccggcgag
     1981 gtcgagagca agggcgtcga gttcgaggtc ggcggcgccc tgacgccgag ctggcaggtg
     2041 atggccggct acacctacgt cgccgccgaa ttcaccaagg acgccgaccg ggccaacatc
     2101 ggccagtcct acgacagcga cctgccgcgc caccagctca agctgagcac cagctaccgc
     2161 ctgcccggcg agctctcgcg ctggcgcatc ggcggcaacg tctatgcgca gaaccggacc
     2221 aagtcgagcg acgacgccaa catccagcaa gccggctacg ccatcgtcgg cctcaacggc
     2281 agctaccagc tcgacgcgcg caccgaaatc cggctcaaca tcaacaacct gttcgacaag
     2341 cactactacc agggcctcgg ctggacgacc ggcggcaacg tcttcggcgc cccgcgcaat
     2401 ttcgtggtga ctgcgcgtta cagtttctga caggccgcct gtcgggcttg atgagcccgc
     2461 ccggaggagg aatatcgaaa cggcgtgcca ttttgcgggc cgacggggtg ttttgagcag
     2521 cttgtcggta attttcatgg tttctcaacg ccacaggccg cggggattgc agttgtttca
     2581 gcaactgcag tccggcattt gagccgtaag gcctgatcgg catccccgtc aaattcatcc
     2641 ttcccgaatg cgcggcggcg aaatattgtg gcaacgagtt gacttttagt tggtaacggt
     2701 tatcatttgt gtcaatgaca ttgcgcaaga ttgatgacag cgcaaactgc caccgccgcc
     2761 tgccaaagcg gtatgggcac acaagacagt cgccagccag ccctcgggca gccagccaga
     2821 ttcctcgaat ttcgatctgc tgcatggccg actggacgcc ggcatgacgc ggatcctgca
     2881 caggagtacc gctgggtgca taccaacaag gatgttctcg atcgtttcca gagcctttac
     2941 gaaggcctga tcgcgcacga cggcttcggc gaaatgcggc tcgagttccg catcctcaag
     3001 cgcggccaga aagaagtcgt catccactgc ggcaaacagt accgcttcgt cgtcgactat
     3061 cgcccgaaca acccgctcgc ctgggaagtg cgacgcgagc ctgccaccgc ggaaagcccg
     3121 cggccgaccc cggagcgccg ccgccgtcag gaggccatcg cctttcccga ccgccgccag
     3181 cggtaacaga catcaagccc cggtcgtcgg cgaccgggca agctcgcaag ccatcggcga
     3241 ggggtcgagg cagatattgg cagtaaagca atgcaggttg tgcgcaccgg gggacggtgg
     3301 gcgacggcaa tcccacttat ctatggagtt ttgactatga gcaacatgct gtctcgcctg
     3361 atgggcaaaa aggacgccga aatccaggcg atcgtcgcca acgttgacgc cgccgccgcg
     3421 ccgcgcggcc tgcgccgcaa gttcgagaag atccgcagca agaaacaggg cggtttcacc
     3481 ctgctcgaac tgctggtcgt cgtcgccatc ctcgccgcga tcgccggcac cgccacgatc
     3541 atgctgcacg ataccgacaa gaaggccttc gccgccgccc acgtggcgat gatggacgaa
     3601 ctgtcgaaag gcatcctgag cttccagcaa ctgaacgccg gcaagtatcc gaatgcctgg
     3661 gattcgctga tgaccaacgc caccggcgtt ctcgacgcta ccgcgacgcc gagcatcctg
     3721 ctcaacgaag gcctcgccga caacctcgcc cagcacgtgc tgaccaccga ggaattcaat
     3781 gcccttgaag ctgtcggcat cacccagctt cgcgtgctca acgaagccgc caccggcatc
     3841 cccgaccagg gcaccctgac ttgtgccaac accgaagccg gcatccgcgc gctgatcgag
     3901 agcaaggcca acaacgtgac ctcgcagaat atcttccgca gccccgcttc caacggttgc
     3961 ggcgccgcgg cgacgatcgc cgacggcaac gcactgctga tgtgggacca ggcccagaac
     4021 gaacgggtca atgccaaggc taccgacgtg ctgatcgcct tcggcgtcgg cccggattcg
     4081 acgctgttcc agtccgccaa ctacggcgcc ctgaccaaca cgccgatcta ccgccacgtc
     4141 gaaggcaacg agtacaaccg cttcgtcgcg ctgttcaacg ttacttccga ctcgaccaag
     4201 ccgaccaatg gcaaggccac cttccaggcc atcgttgacg gtgccggcga caccaaggac
     4261 gaggaactcg gcgaattcga caacgttcgc ccgacctaag cctcacctga gctgaacccg
     4321 ggcgctggcg gccgaatccg ccggcgccct tcttccagca acacacccga gaatcccatg
     4381 acgctgtcca aggcaggcga actgcgcgac tgcggtttca ccctgatcga actcctggtt
     4441 gtctgcagcc tcctcgctgc cctctcctat accgcctggg gcgcctacac cggcgtccag
     4501 gaacgcgccg aggacgatat cgcccgcgcc gagatgctgc gcctggccga cgcgctcaag
     4561 cgcttccgcg ccgataccgg ctattacccg ggccaggggc cgttcgcgct ggccgccccg
     4621 ggcaccgtcg aaaccgccag cggcgacggc tacacctgcg accccgtcgg cggcgtgcgg
     4681 cgcagttggg ccgcgcccgc gctcgacgcc gacaaggacg cctggttcgc ctcgccggcc
     4741 aacctcgccc tgctcttcgc agcaccggcg ctctgcgcca accatccgct ggcttacctg
     4801 gaacgctggc agcccgccag cggtcgcggc tggcacggcc cctatctgga catttccgcc
     4861 cgcgcctggg tcgaccatgg caacgacttc aacgccgacc gcaactgcgc cctgaacggc
     4921 gcccccgatg gcgacggcaa cccggtcgcc ggcagcaaga tcgtcgacat cccggccttc
     4981 ggcaacggtc cgcgcttcag cgccgtcggc ccggcctggg aaccctgcgc cgagcaagcg
     5041 cacagcacgc caagcgcaac ctgcatgctc ggctggcgga gcgtcccgcg ccaggccgcc
     5101 ggctacgccg cgatgcgcca cgaactaccc gcccacgccc gccccttcgt cgtcttcggc
     5161 ctggccgaca acgatgcccc gcgcctcgtc tatttcgggc gcgatggccg ctacggcggg
     5221 cgcaacgccg ccgacccctg tcttcccaac cttcccgatc ccgatggcgc cgatgaccag
     5281 gtgctgtgcc tgaatcgctg agcgccccct tcactgatga aaaaccaccg cttcctcgcc
     5341 atctttgccc tcgccgccat cgtcgccctg ccgctgcatg ccgccgaccc ggcgcagcag
     5401 attccggtca gccgcctcga cccggccgcc gcctacaagg acgtcgacct caag
//
LOCUS       DAOSDX010000060         5411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5397_length_5411_cov_3.234130, whole genome shotgun sequence.
ACCESSION   DAOSDX010000060 DAOSDX010000000
VERSION     DAOSDX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5397_length_5411_cov_3.234130"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="PLN02_02130"
     CDS             complement(<1..1561)
                     /locus_tag="PLN02_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOI51148.1"
                     /translation="MPPPAPTDFHALSAAQVLSGLRATRAGLDDDEAARRLATHGPNR
                     LPAPPRDSLLRRFAKHFQDILILILIGAAVITAMLHHWIDTGVILGVVVINAIIGFLQ
                     EGKAEAALAGIRKMLSASAQVRRQGKWRQIDAAELVPGDIVRLRSGDRVPTDLRLLEA
                     TNLRIEESALTGESVPSDKRIDPVAADAGIGDRACMAYSGTLVAAGRALGVVIATGAS
                     TEIGRINRLIAEVETLQTPLTRQMAQFGRQLSVLIVGLAALMLVVGIGLHGLPLGEVL
                     LAAIGFAVAAIPEGLPAILTITLALGVQRMAQRQAITRRLTAVETLGSVTVICSDKTG
                     TLTKNEMTARHVITRAGNYLVSGSGYAPQGDITLDGQFVELAEHAHLHALIEVIAVAN
                     DTHVAENAGQWCVTGEPTEGALRTLAHKAGFSSGQHTRHATIPFESANKFMATLASTP
                     TGLRRILLKGAPDRLLDRCREELGADGQPVPLDRAWWEREIDRLSQEGLRVLAAAARR
                     VDDAHVRLSEND"
     gene            complement(1583..2041)
                     /locus_tag="PLN02_02135"
     CDS             complement(1583..2041)
                     /locus_tag="PLN02_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOI51149.1"
                     /translation="MNFEHLIVINDPGNPLIVPLNRRQVWEGLLQRVYHPLPFLPGLE
                     SCTLLQRDDAFLRRELDFGAARICDRVTMAVEEWVHFEIEPGEQHPGGSLTIRIEEPA
                     PEQLCLRFIYATALAAEDQAYIDYIKSAYQQSDIDCVQIIRTLAADGPRQ"
     gene            2064..2771
                     /locus_tag="PLN02_02140"
     CDS             2064..2771
                     /locus_tag="PLN02_02140"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HOI51150.1"
                     /translation="MQLERILQKHGFGTRKACRAMIRDERVAIAGNLLADPFADIPTA
                     GLVFTVDDVDWPFCEFASVLLHKPAGYECSRKPQHHPSVLELLPLPLRERGVQPIGRL
                     DEDTTGLLLLTDDGQLNHQLSSGKRKVPKVYLATVKHPLDAGQIGQLLAGVLLHDEPQ
                     PVAAAAAEIVDELHLRLTVTEGKYHQVKRMVAAVGNRVEALHREALAELILPPDLAPG
                     QWRWLTAEDYQNLGVAR"
     gene            2768..3682
                     /locus_tag="PLN02_02145"
     CDS             2768..3682
                     /locus_tag="PLN02_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51151.1"
                     /translation="MTLTEMRYIVALARERHFGRAAEACHVSQPTLSVALKKVEGQIG
                     AALFERTATDVRITTLGERIVEQATRVLAEAVKLEEIAEATGDPMSGQLRVGIIYTIA
                     PYLLPQLIPALSRQAPNMPLFLKEDFTANLIPALKSGELDVIIIALPFAEPGLVAQAV
                     YEEPFRVVVPANHPWAGYEAIDADELDGQNLLLLGQGNCFRDQVLESCPRLNGQDALA
                     HSLEGSSLETIRYMVASGAGVAVMPSSAADPLLDKEPLVRVLPFVGTPPSRTVGLVWR
                     VTYPRSQAIDAMRAAVLSCQLPGATARR"
     gene            3691..>5411
                     /locus_tag="PLN02_02150"
     CDS             3691..>5411
                     /locus_tag="PLN02_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51152.1"
                     /translation="MKPRRLLLAFLFCLAGSQSASAAPERVRVVSDENFPPYIFLGAD
                     GTPEGYLVDLWKLWEKQTGIRVELTATAWAEAQAMISRGEADVIEAIYRTPGREGSYE
                     FSQPYSTQHIYIFADRSISGISGPNTLKGFQIGVEQGDACIEMLERQGITSLQRYRDY
                     SQMIQAAIDQEVKLFCLDEIPANFYLYRMHAQERFQKTFQLYSGQMHRAVRKGEGELL
                     AVVEAGMAAINPADVEALREKWMGQPLRLAPYARYFTLAIVGLLLLGGLLLLWVRTLR
                     RQVRQRTTELDLRRAQLATLVDTIPDLVWLKDAKGVYLACNRRFEKLYGHLESAIVDR
                     TDYDFVDRETADFFRARDEAAIAAGGPVSNEEWLTFADGGERLLFETIKTPMLDSDGQ
                     LIGVLGVAREITARRRAEDALRASEEKLRIAQSSAALGIWEVNLAVGVTYWSPEVEAM
                     YGIAIGSFDGRQASWLARVHPDDRSAVLQAVGEYMQRNEAFDLAFRIVRPDGEVRWIA
                     SRGQVHRSAQGAAERIIGVNFDITEKKRTEEELQHYRDQLEELVDERTRQLAQARDAA
                     EAANRAK"
BASE COUNT          894 a         1676 c         1888 g          953 t
ORIGIN      
        1 ggtcgttttc gctcaggcgg acgtgcgcgt catcgacgcg gcgggcggcg gcggcgagca
       61 cgcgcaggcc ttcctggctg aggcggtcga tctcgcgttc ccaccaggcc cggtcgagcg
      121 gcaccggctg gccgtcggcg ccgagctctt cgcggcagcg gtcgagcagg cggtcggggg
      181 cgcccttgag caggatgcgg cgcaggccgg tcggcgtgct ggcgagcgtg gccatgaact
      241 tgttggccga ctcgaagggg attgtcgcgt gccgggtatg ttggcccgac gaaaatcccg
      301 ccttgtgcgc cagggtgcgt agtgcgcctt cggtcggttc gccggtgacg caccattggc
      361 cggcgttttc cgcgacatgg gtatcgttgg cgacggcgat gacctcgatc aacgcgtgca
      421 gatgggcgtg ttcggccagt tcgacaaact gcccgtcgag cgtgatgtcg ccctgcggcg
      481 catagccgct gccgctgacc aggtagttgc cggcgcgggt gatgacatgg cgggcggtca
      541 tctcgttctt ggtcagggtg ccggtcttgt ccgagcagat gacggtgacc gaacccagcg
      601 tttcgacggc ggtcagccgg cgggtgatcg cctggcgttg cgccatgcgc tgcacgccga
      661 gcgccagcgt gatggtgagg atggccggta gcccttcggg aatggcggcg acggcgaagc
      721 cgatggcggc gagcaggact tcgccgagcg ggaggccgtg gagaccgatg ccgaccacca
      781 gcatcaacgc ggcaaggccg acgatgagca ccgacaactg cctgccgaac tgcgccatct
      841 gccgggtcag cggtgtctgc agggtttcca cttcggcgat caggcggttg atgcgaccga
      901 tttcagtgct ggcgccggtg gcgatgacga caccgagtgc ccggccggcg gcgaccaggg
      961 taccggaata ggccatgcag gcgcggtcgc cgatgccggc gtcggcggcg accgggtcaa
     1021 tgcgcttgtc actgggcacc gattcgccgg tcagtgccga ttcctcgatg cgcaggttgg
     1081 tcgcttccag caggcggagg tcggtcggga cgcggtcgcc ggagcgcagg cggacgatgt
     1141 cgccgggtac cagttcggcg gcgtcgatct gccgccattt cccctggcgc cggacctggg
     1201 cgctcgccga caacatcttg cggatgccgg ccagcgccgc ctcggccttg ccttcctgga
     1261 ggaagccgat gatcgcgttg atgacgacga cgccgagaat gaccccggtg tcgatccagt
     1321 ggtggagcat ggcggtgatt accgcggcgc cgatcaggat gaggatcagg atgtcctgga
     1381 agtgcttggc aaagcgcctg aggaggctgt cgcggggtgg cgcgggcagg cggttggggc
     1441 catgcgtggc gagccggcgg gctgcctcgt cgtcgtccag gccggcgcgc gtggcccgca
     1501 aaccgctcag tacctgggcg gcgctcaggg catggaagtc ggtgggggcg ggcggcggca
     1561 ctggccttcc tgtggcgccg gcctactggc gggggccgtc ggcggccagg gtgcggatga
     1621 tctgtacgca gtcgatgtcg gactgctggt aagccgattt gatgtaatcg atgtaggcct
     1681 ggtcttcggc ggcgagcgcg gtggcgtaga tgaaacgcag gcagagttgc tcaggcgccg
     1741 gttcctcgat gcggatggtc aggctgccgc cggggtgttg ttcgccgggc tcgatctcga
     1801 aatgcaccca ttcttcgacc gccatggtga cgcggtcgca gatgcgggcg gcgccgaaat
     1861 cgagttcgcg gcgcaggaag gcgtcgtcgc gctggagcag ggtgcaggac tcgaggccgg
     1921 gcaggaaggg cagcggatgg taaacccgct gcaatagccc ttcccagacc tggcggcggt
     1981 tcagcggaac gatcagcgga ttgccgggat cgttgatgac gatcaggtgt tcgaagttca
     2041 tgcccagatg gtagcatcgt cacatgcaac ttgaacgtat cctgcaaaag cacggctttg
     2101 gaacgcgcaa ggcctgccgg gcaatgatcc gcgacgagcg ggtggcaatt gccggcaatc
     2161 tcctggccga tcccttcgcc gatatcccca ccgccggact ggtgttcacg gtcgacgatg
     2221 tcgactggcc gttctgcgag ttcgccagcg tcctgctgca caagccggcc ggctacgagt
     2281 gctcgcgcaa gccgcagcac catccttcgg tccttgaact gctgcccttg ccgctgcgcg
     2341 agcgcggcgt ccagccgatc ggccgcctcg acgaggacac caccggcctg ctgttgctca
     2401 ccgacgacgg ccagttgaac catcagctat cgtcgggcaa acgcaaggtg cccaaggttt
     2461 atctggcgac ggtgaaacat cctctcgatg ccgggcagat cgggcaactg cttgccggtg
     2521 tgctgctcca tgacgagccg caaccggtgg ccgcggccgc cgccgaaatc gttgatgaac
     2581 tgcacttgcg cctgacggtg accgaaggca agtatcacca ggtcaagcgc atggtcgccg
     2641 ccgtcggcaa ccgggtcgag gccttgcacc gcgaggcgct ggccgagctg atcctgccgc
     2701 cagacctggc acccggacag tggcgctggc tgacggcaga ggattatcaa aacctgggag
     2761 tcgcacgatg accctgaccg agatgcgcta catcgttgcc ttggctcgcg aacgccattt
     2821 cggccgggcc gccgaggcct gtcatgtcag tcagccgacg ctgtcggtgg cgctgaagaa
     2881 ggtcgagggg cagatcggcg ccgcgctgtt cgagcgtacc gcgaccgatg tccgcatcac
     2941 cacgctgggc gagcgtatcg tcgagcaggc gacgcgcgtc cttgccgagg cggtcaagct
     3001 ggaagaaatc gccgaggcca ccggcgaccc gatgagcggc cagttgcgcg tcggcatcat
     3061 ctacaccatc gccccctatc tgctgccgca actcatcccg gcactcagcc ggcaggcgcc
     3121 gaacatgccg ctgttcctca aggaggactt caccgccaac ctgattccgg cgctgaaatc
     3181 cggcgagctc gacgtcatca tcattgccct gccgttcgcc gagccggggc tggtggcgca
     3241 ggcggtatac gaggagccgt tccgcgtcgt cgtcccggcc aaccatccct gggccgggta
     3301 cgaagccatc gacgccgatg aactcgacgg tcagaatctg ctgctcctcg gccagggcaa
     3361 ctgtttccgc gatcaggtcc tggaatcctg cccgcgcctc aatggccagg atgcgcttgc
     3421 ccattcgctc gaaggcagtt cgctggagac cattcgctac atggtggcga gcggcgccgg
     3481 tgtcgccgtg atgccaagtt cggccgccga tccgctgctc gacaaggagc cattggtacg
     3541 cgtcctgccc ttcgtcggca cgccgccgtc gcgcaccgtc ggtctggttt ggcgggtgac
     3601 ctacccgcgc tcgcaggcga tcgacgcgat gcgggcggcg gtcctgtcct gccagttacc
     3661 cggcgccacc gccagacgct gaggtttcac atgaaaccgc gccgacttct cctggccttc
     3721 cttttttgtc ttgccgggag tcagtcggca agtgccgccc cggagcgggt cagggtggtc
     3781 agcgacgaga attttccgcc ttacatcttc ctcggtgccg atggtacgcc tgaaggttac
     3841 ctggtcgatc tctggaaact atgggagaag cagaccggca tccgcgtcga gttgacggcg
     3901 acagcctggg ccgaggcgca ggcgatgatc agccgtggcg aggccgatgt catcgaggcg
     3961 atctaccgga cgccggggcg cgagggcagc tatgaatttt cccagcctta ttcgacccag
     4021 cacatctaca tcttcgccga tcgttcgatc agcggcatca gcggaccgaa cacgctgaag
     4081 ggctttcaga tcggtgtcga gcaaggcgac gcttgcatcg agatgctgga acgccaggga
     4141 atcaccagcc tgcagcgcta tcgcgactat tcgcagatga tccaggcggc gatcgatcag
     4201 gaggtcaagc tgttctgcct cgacgagatt ccagctaatt tctatctcta ccggatgcac
     4261 gcgcaggagc gtttccagaa gactttccag ttgtatagcg ggcagatgca ccgcgcagtg
     4321 cgcaagggcg agggcgaact gctggcggtg gtcgaggccg gcatggcggc aatcaatccg
     4381 gccgatgtcg aggctctgcg cgagaaatgg atgggccagc cgctgcgctt ggcgccctac
     4441 gcgcgttatt tcaccctggc gatcgttggt ctgctgctgc tcggcggttt gctgctgctc
     4501 tgggtccgca ccctgcgccg gcaggtgcgg caacgcacta ccgaactgga cctgcggcgc
     4561 gcccaactgg caacgctggt cgatacgatt cccgatctgg tctggttgaa ggacgccaaa
     4621 ggcgtctatc tcgcgtgcaa ccggcgcttc gaaaagctgt acggacatct cgaatcggcg
     4681 atcgtcgatc gtaccgacta cgacttcgtc gatcgcgaga ctgccgactt tttccgggcc
     4741 cgggatgagg cggcgattgc cgccggtggc ccggtgagca acgaggaatg gctcactttc
     4801 gccgatggcg gcgagcgcct gctgttcgaa acgatcaaga cgccgatgct cgacagcgac
     4861 ggccagctta ttggggtgct cggcgttgcc cgcgagatca ccgctcgccg gcgggcggag
     4921 gacgcgttgc gggcgagcga ggaaaagctg cgcattgccc agtcgagcgc tgcgctcggc
     4981 atctgggagg tcaatctggc ggttggcgtg acctactggt cgccggaggt cgaagcgatg
     5041 tacggcatcg ccatcggcag tttcgatggc cgtcaggcaa gctggctggc gcgcgtgcac
     5101 cccgacgatc gttccgcggt gctgcaggcg gtgggggagt acatgcagcg caacgaggca
     5161 ttcgatcttg ccttccgcat cgtccgaccg gatggcgagg tgcgctggat cgcctcgcgt
     5221 gggcaggtgc atcgttccgc gcagggggcg gcggagcgca tcatcggcgt caatttcgac
     5281 atcaccgaga agaaacggac cgaagaggaa ctgcaacact atcgggacca gctcgaggaa
     5341 ctggtcgacg agcggacccg ccaactggcg caggcccgtg acgcggccga ggcggcaaat
     5401 cgcgcgaaaa g
//
LOCUS       DAOSDX010000061         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5509_length_5315_cov_3.476046, whole genome shotgun sequence.
ACCESSION   DAOSDX010000061 DAOSDX010000000
VERSION     DAOSDX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5509_length_5315_cov_3.476046"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2995)
                     /gene="fdnG"
                     /locus_tag="PLN02_02155"
     CDS             complement(<1..2995)
                     /gene="fdnG"
                     /locus_tag="PLN02_02155"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOI51153.1"
                     /translation="MNINRRQFFKISAASLSSSGLVALGMTPTAAQAEVRQYKLFRAT
                     ETRNNCTYCSVGCGILMYSLGDGAKNAKKKIFHVEGDPDHPVSRGSLCPKGAGVLDFI
                     HSEQRLKFPEVREAGSNAWKRISWDEAIERIARLMKDDRDANFIEKNEKGQLVNRWTT
                     TGFLGSSAASNETGILDVKFARALGMVHLENQARLCHGPSVAGLAPSFGRGAMTNNWV
                     DIKNANVILVMGGNPAEAHPVGFKWAVEAKVRNKAYMMVVDPRFNRTAAVADFYSPIR
                     AGADSAFLLGVANYLLGKDKIQHEYVKAYTNAPLIVREDYKFEDGLFSGYDAEKRSYD
                     RSSWAYELGADGYALRDETLQHPRCVFNLLKKHVERYTPEMVSSICGTPKEDFLHVCE
                     KLASTCVPDKTTSILYALGWTHHVGGSQMIRASAIVQLLLGNIGMPGGGINALRGHSN
                     VQGFTDLGVLTTMLPGYLTLPNENQETLQTYLEQRTPKTLVDKQVNFWKNTPKFMVSF
                     LKGMYGDKATPENNFGYDWLPKWDRSYDVMAFVEQMHKGKANGYIIQGFNVIAAAASS
                     GKVREGLAKLKYLVVMDPLQTETSSFWQNHGEFNNVNPADIQTTVFRLPSSCFAEENG
                     SIANSGRWLQWHWAAQTPPHEAWHDGRFLGTLLMKLRELYAKEGGVCPEPLINVDWNY
                     HDPKDPHPEEVAKQLNGYALADLFDDKGNQIAKKGELLSSFAHLRDDGTTTSLCWIYT
                     GQWTQAGNMMARRDNTDTGLGNTPGWGFAWPANRRLLYNRASANPAGEPWDPKRKFLH
                     WDGAKWGGADVPDYPPTAAPGSGVSPFIMLQDGVGHLFSMGAMTDGPFPEHYEPEESP
                     IPANPLHSGSGKSPVARVFKDDRDSFGSREEFPYVATSYSITEMFRHWTKHSLLNAIV
                     QPEQFVEISEKLAASKGIKAGDLIKVSSKRGHIRGKAVVTKRVMTLQVAGQEVEQVGI
                     PVHWGFEGTARKGYLANTLSPRVGDA"
     gene            complement(3228..4532)
                     /locus_tag="PLN02_02160"
     CDS             complement(3228..4532)
                     /locus_tag="PLN02_02160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI51154.1"
                     /translation="MSFAKLGLLPPLTQTLADLGYAAPTAIQQQAVPAVLAGRDVLAA
                     AQTGTGKTAAFLLPLLQMLAATPAAGPAPRAVVLVPTRELAEQVLASCQRYGAGLPLR
                     CYALYGGVSHGPQLTALRDGVDLLVATPGRLIDLFGKGELRLREVRHVVLDEADRMLD
                     LGFADDLATVFQALPRERQVLLFSATFADAVRALAQARLRKPLSIEATPRNTAARTIR
                     QWVVPVDKKRKPELFLFMWRDRGWQQVLVFVKTKLGADELAERLRGKGIAAEAIHGDR
                     PQASRQAALAAFKAGTVAVLVATDVAARGLDIDGLPQVVNFDLPLQAEDYVHRIGRTG
                     RAGAAGEAISFVCADEAPQLAAIESLLKKKLSRDEEPGFEPRHQVPATLGAAPPRRPP
                     PLAKAGAGRGRTPGNWVGFDERPARGAGRGRPGGAKGSRGGR"
     gene            4682..>5315
                     /locus_tag="PLN02_02165"
     CDS             4682..>5315
                     /locus_tag="PLN02_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI51155.1"
                     /translation="MDDKSPADAPAAAPQQPFTILVVDDEPGMISFLQRALAQRYGQV
                     DTAGSVEQAIPQLRRRLYDLIVLDISLPGRSGIEWLHELRDDGFVGDVILITAYADLD
                     TAINALRAGASDFLLKPFSVAQMLNGVARCFERSRLRRENFVLRREIEERSTAIDGVI
                     CESDAMQTVCERLKRIAPTNATVLVTGESGTGKEVAARALHRMSARHAGPF"
BASE COUNT          803 a         1784 c         1807 g          921 t
ORIGIN      
        1 tggcgtcgcc gacgcgcggg ctcagcgtgt tggccaggta gcccttgcgc gccgtgccct
       61 cgaagcccca atgcaccggg atgccgacct gctcgacttc ctgccccgcc acctgcagcg
      121 tcatcacgcg cttggtcacc accgccttgc cgcggatgtg accgcgcttg ctcgacacct
      181 tgatcaggtc gccggccttg atgcccttgc tcgccgccag cttctcgctg atttcgacga
      241 actgctcggg ctggacgatg gcgttgagca gcgagtgctt ggtccagtgg cggaacatct
      301 cggtgatcga gtacgaggtg gcgacgtagg ggaactcctc gcggctgccg aaactgtccc
      361 tgtcgtcctt gaacacgcgc gccaccgggc tcttgccgct gcccgaatgc agcggattgg
      421 ccggaatcgg gctttcctcg ggttcgtagt gctcggggaa cggcccatcg gtcatcgcgc
      481 ccatcgagaa caggtggccg acgccgtcct ggagcatgat gaacggactg acaccgctgc
      541 ccggcgccgc cgtcggcgga tagtcgggaa catcggcacc gccccacttg gcgccgtccc
      601 agtgcaggaa cttgcgcttc gggtcccacg gctcgccggc cgggttggcc gaggcgcggt
      661 tgtacaacag gcgacggttg gccggccagg cgaagcccca gcccggcgtg ttgccgaggc
      721 cggtgtcggt attgtcgcgg cgggccatca tgttgccggc ctgcgtccat tgcccggtgt
      781 agatccagca caggctggtc gtcgtgccgt cgtcgcgcag gtgggcgaag ctcgacagca
      841 gctcgccctt cttggcgatc tggttgccct tgtcgtcgaa caggtcggcc agcgcgtagc
      901 cgttgagctg cttggcgact tcttccggat gcgggtcctt gggatcgtgg tagttccagt
      961 ccacgttgat cagcggctcg gggcagacgc cgccttcctt ggcgtagagt tcgcgcagct
     1021 tcatcagcag ggtgccgagg aagcggccgt cgtgccaggc ttcgtgcggc ggcgtctgcg
     1081 ccgcccagtg ccattgcagc cagcggccgg agttggcgat cgaaccgttc tcctcggcga
     1141 agcaggacga cggcaggcgg aacaccgtgg tctggatgtc ggccggattg acgttgttga
     1201 actcgccgtg gttctgccag aacgacgagg tttcggtctg cagcgggtcc atcaccacca
     1261 ggtacttgag cttggccagg ccttcgcgca ccttgcccga cgacgcggcg gcggcgatca
     1321 cgttgaaacc ctggatgatg tagccgttgg ccttgccctt gtgcatctgc tcgacgaagg
     1381 ccatcacgtc gtagctgcga tcccacttgg gcagccagtc gtagccgaag ttgttctcgg
     1441 gcgtggcctt gtcgccgtac atccccttga ggaagctgac catgaacttg ggcgtgttct
     1501 tccagaagtt cacctgcttg tcgaccaggg tcttcggcgt gcgctgttcg agataggtct
     1561 gcagcgtttc ctggttctcg ttgggcagcg tcaggtagcc cggcaacatg gtggtgagca
     1621 cgcccaggtc ggtgaagccc tggacgttgg agtggccgcg cagggcattg atgccgccgc
     1681 cgggcatgcc gatgttgccg agcagcaact ggacgatggc cgaggcgcgg atcatctgcg
     1741 agccgccgac gtgatgggtc cagccgagcg cgtagaggat gctggtggtc ttgtccggaa
     1801 cgcaggtcga agcgagtttc tcgcagacgt gcaggaagtc ttccttgggc gtgccgcaga
     1861 tgctgctgac catctccggc gtgtagcgct cgacgtgctt cttgagcagg ttgaagacac
     1921 agcgcggatg ctgcagcgtc tcgtcgcgca acgcgtagcc gtcggcgccc agctcgtagg
     1981 cccaggagga acggtcgtag ctgcgtttct cggcgtcata gccgctgaac aggccatcct
     2041 cgaacttgta gtcctcgcgc acgatgagcg gcgcgttggt gtaggccttg acgtactcgt
     2101 gctggatctt gtccttgccc agcaggtagt tggcgacgcc gagcagaaag gccgaatcgg
     2161 caccggcacg gatcggcgaa tagaagtcgg cgacggcggc ggtgcggttg aagcgcggat
     2221 cgacgaccat catgtaggcc ttgttgcgca ccttggcctc gaccgcccac ttgaagccga
     2281 ccgggtgggc ctcggccggg ttgccgccca tcaccagaat gacgttggcg tttttgatat
     2341 cgacccagtt gttggtcatc gcaccgcgcc cgaaggacgg cgccaggccg gccaccgacg
     2401 ggccgtggca gagacgggcc tggttctcca ggtgcaccat gcccagcgcg cgggcgaact
     2461 tgacgtcgag gatgccggtt tcgttgctcg ccgccgacga gccgaggaag ccggtggtcg
     2521 tccagcgatt gaccagttgt cccttctcgt tcttctcgat gaagttggcg tcgcggtcgt
     2581 ccttcatcag gcgggcgatg cgctcgatcg cctcatccca ggagatacgc ttccatgcgt
     2641 tgctgccggc ctcgcgcact tccggaaact tgaggcgctg ctcgctgtgg atgaagtcga
     2701 gcacgccggc acccttcggg cacagcgagc cgcggctgac cgggtgatcc gggtcgccct
     2761 cgacgtggaa gatcttcttc ttcgcgttct tggcgccgtc gcccaggctg tacatcagaa
     2821 tgccgcagcc gaccgagcaa taggtgcagt tgttgcgcgt ctcggtggcg cggaacaact
     2881 tgtactggcg gacttccgcc tgggcggcgg tcggcgtcat gccgagcgcg acgaggccgg
     2941 aactgctcag gctggccgcc gaaatcttga aaaactgccg tcgattgatg ttcattttgt
     3001 ccttccctcc tggtcggccc tggcggccac ggggaaaaac catggcaacg accgtgccaa
     3061 gacaggcaag tcattgaact aaatgaaatc gacgccatac ggtgaaacaa atcccctttg
     3121 gcggaatgga cataatgtcc caacggcaaa gcgcgcgcgg gacaaaatgg gacagcttgg
     3181 ggcgcgttcg cagtaagcgg cgacccgctg cctgccgcaa acgcgcccta gcgtccgccg
     3241 cgactgccct tggcgccacc cggccggccc cggccggcgc cgcgggcggg acgttcgtcg
     3301 aagccgaccc agttgcccgg cgtcctgccc cgcccggcgc cggccttggc cagcggcggc
     3361 gggcggcgcg gcggcgccgc cccgagcgtc gccggcacct ggtggcgcgg ctcgaaaccc
     3421 ggttcctcgt cgcggctgag cttcttcttc agcaggcttt cgatggccgc cagttgcggt
     3481 gcctcgtcgg cgcagacgaa ggagatcgcc tcgccggcgg caccggcgcg cccggtgcgg
     3541 ccgatgcggt gcacgtaatc ctcggcctgc agcggcaggt cgaaattgac cacctgcggc
     3601 agcccgtcga tgtccaggcc gcgcgcggcg acgtcggtgg caacgaggac cgccaccgta
     3661 cccgccttga acgccgccag cgctgcctga cggctggcct gcggccggtc gccatggatc
     3721 gcctcggcgg cgatgccctt gccgcgcagg cgctcggcca gttcgtcggc gcccagcttg
     3781 gtcttgacga agaccagcac ctgctgccag ccgcggtcgc gccacatgaa caggaatagc
     3841 tcgggcttgc gcttcttgtc gaccggcacc acccactggc ggatcgtccg cgccgcggtg
     3901 ttgcgcggcg tcgcctcgat gctcagcggc ttgcgcaggc gcgcttgggc cagcgcccgc
     3961 accgcgtcgg cgaaggtggc cgagaacagc agcacctggc gctcgcgcgg cagcgcctgg
     4021 aagacggtcg ccaggtcgtc ggcgaagccg aggtcgagca tgcggtcggc ttcgtcgagg
     4081 acgacatggc ggacttcgcg caggcgcagc tcgcccttgc caaacaggtc gatcagccgg
     4141 cccggcgtcg ccaccagcag atcgacgccg tcgcgcaacg cggtcagttg cggcccgtgg
     4201 ctgacgccgc cgtaaagcgc gtagcagcgc agcggcaggc cggcgccgta gcgctggcaa
     4261 ctggccagca cctgctcggc gagttcgcgc gtcggcacca ggacgacggc gcgcggcgcc
     4321 gggccggcgg ccggcgtggc ggcgagcatc tgcagcagcg gcaggaggaa ggcggcggtc
     4381 ttgccggtac cggtctgcgc cgcggccagc acgtcgcgcc cggccagcac cgctggcacg
     4441 gcctgttgct ggatcgccgt cggcgcggcg tagccgagat cggcgagggt ttgcgtgagc
     4501 ggcggcagga ggccgagttt ggcgaagctc atggcggctt gtcccggtgg aaaagcgcga
     4561 ttttacccgg tcgaccggcg gcctccgcca gaaccggcga gcggcaacga cggccggcgg
     4621 tcgcctgcgt ttgcgtcacc ggccgctgct ttgcgatcat gcgcgctacg cggagaaaaa
     4681 catggacgac aaatccccgg ccgacgcccc cgcggccgcc ccccagcaac ccttcaccat
     4741 cctcgtcgtc gacgacgaac cgggaatgat cagcttcctg cagcgcgcgc tggcccagcg
     4801 ctacggccag gtggacaccg ccggcagcgt cgagcaggcg attccccaat tgcgccgccg
     4861 gctctacgac ctgatcgtcc tcgacatctc gctgcccggc cgctccggca tcgaatggct
     4921 gcacgaactg cgcgacgacg gcttcgtcgg cgacgtcatc ctgatcaccg cctacgccga
     4981 cctcgacacc gccatcaacg cgctgcgcgc cggtgcttcg gatttcctgc tcaagccctt
     5041 ctcggtcgcc cagatgctca acggcgtcgc ccgctgcttc gagcgttcgc gcctgcgccg
     5101 ggagaatttc gtgctgcgcc gcgagatcga ggaaagatcg acggcgatcg acggcgtcat
     5161 ctgcgagtcc gacgccatgc agaccgtctg cgagcgcctc aagcgcatcg cgccgaccaa
     5221 cgcgaccgtc ctcgtcaccg gcgaatccgg taccggcaag gaggtcgccg cgcgcgccct
     5281 gcaccggatg agcgcccgcc acgccggccc cttcg
//
LOCUS       DAOSDX010000062         5307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5519_length_5307_cov_3.582254, whole genome shotgun sequence.
ACCESSION   DAOSDX010000062 DAOSDX010000000
VERSION     DAOSDX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5519_length_5307_cov_3.582254"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLN02_02170"
     CDS             <1..853
                     /locus_tag="PLN02_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI51156.1"
                     /translation="GTWIAGRVIAQELSAPLAVAALRFVVAGLVVGGVMLVSGGGIAL
                     PKDRQEWGLVWALGFFGIFLYGLCFFFGLQHIPAGRGALVVALNPVVIVLLAWAIGKE
                     GMNRRKALGSAIALAGCLTVIGNGDPLALLQGTVGIGEWLIVGCVLSWAAYTFIGWRA
                     TGHFSALATTLYASFSGAFLLGLAALVQGDVDFAGWSWRVWSSMLFLAVFGTAIAYTW
                     FTAAVQQLGAGHASVFINLVPVFAVLQAAVLLDERLGLPVLFGGLLVIAGVWLTTVQK
                     TTLEKTA"
     gene            850..1986
                     /locus_tag="PLN02_02175"
     CDS             850..1986
                     /locus_tag="PLN02_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOI51157.1"
                     /translation="MILTQEQEMIRDTMRAFAQERLAPFAAEWDKNHTFPADALKELG
                     ELGAMGMCVPEEWDGAGMDYMSLVLTLEEIAAGDGATSTIVSVQNSLACGITQKYGTD
                     AQKEEWLKPLARGEKWGCFCLTEPHTGSDAAAITTRADRDGDHFVLNGVKQFITTGKH
                     AHMAIVFAVTDKAAGKKGISCFLIPTSTPGFVVGRTEDKMGQHASDTVQIILENCRVP
                     ASALLGKEGEGYKIALSNLEAGRIGIAAQSIGMARAAFEAAVRYAKERVTFGVPIIDH
                     QAVNFKLADMNTLLDAARLMVWRAAQLKDAGKPCLKEASMAKMFASEAAEKIASDAIQ
                     IHGGVGYTSDFPVERIYRDVRICQIYEGANDIQRLVIGRSIAAE"
     gene            1995..2804
                     /locus_tag="PLN02_02180"
     CDS             1995..2804
                     /locus_tag="PLN02_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOI51158.1"
                     /translation="MASPKSNSRPDVEEHSLRLAVLIDADNAQASVIEGLLAEIARFG
                     EASVKRIYGDFTSPNSAQWKKVLNQHAIKPVQQFAYTTGKNATDGTMIIDAMDLLYTR
                     RFDGFCLVSSDSDFTGLALRIREEGLSVFGFGEEKTPDAFRNACHKFVFTEVLRALPM
                     TTPPAEPESDEQPIQQTSSSPVAKKALPLRFVLQALDQACDDRGWANLGAFGSYLTKL
                     QSDFDPRLYGFKKLSELVRSKPHLFVVEERQLTGSNSPVIYVRKRPGKGIQ"
     gene            complement(2798..3199)
                     /locus_tag="PLN02_02185"
     CDS             complement(2798..3199)
                     /locus_tag="PLN02_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOI51159.1"
                     /translation="MTLVDSSVWIDYFRGAVTPQTDCLDRLLGSQLIVVGDLILAEVL
                     QGFVSDRDYAQARALLTALPVVPLVGAEIAQQAASNFRQLRARGITVRKTIDTLIATS
                     CIEHGYALLYSDRDFDPFVEHLGLVSAWPGH"
     gene            complement(3196..3390)
                     /locus_tag="PLN02_02190"
     CDS             complement(3196..3390)
                     /locus_tag="PLN02_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOI51160.1"
                     /translation="MRTNIVIDDGLMQAAMQLTGLKTKREAVEAGLKTLLRLAQQEEI
                     RKFRGRLDWQGDLDRMRQDR"
     gene            3465..4082
                     /locus_tag="PLN02_02195"
     CDS             3465..4082
                     /locus_tag="PLN02_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HOI51161.1"
                     /translation="MTQDNINPIDFWFDFSSPYGYLMAEKIDDVAARFGRKVRWHPIL
                     LGVIFQATGSRPPADGVSNKGRYMLHDFARSARFLGVPYKMPSRFPLPTQNAARAYYW
                     LHGQDCALARRFARAVYRGFFVDDLDISAPDTVLDIAAGLGIDCPALAAALQAPEIKA
                     RLKDECEQALAAGVFGSPHVIIDGEAFFGADRLPQIERWLESGGF"
     gene            4085..4903
                     /locus_tag="PLN02_02200"
     CDS             4085..4903
                     /locus_tag="PLN02_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOI51162.1"
                     /translation="MSLRLVLYASLLAIAGWSLAIEPGQVQRRDLTLSTARLKQPLTI
                     AMASDLHVGAPHVSREALRRLVGRINEANPDLILLPGDFMIQGVLGGTQVAPEDLAAD
                     LAGLHARLGVFATLGNHDWRFDGPRVRRALEQVGIRVLENEAIELPESGLWLAGIGDD
                     MTCHAEPAAAFAGVPNGADVIAMLHDPANAPDLPTSTRVALAGHTHGGQVRLPFVGAL
                     IVPGRSAREHAYGWIPGSAVPTWVSSGVGTSILPIRFNCPPEFVVLRLANKESS"
     gene            4900..>5307
                     /locus_tag="PLN02_02205"
     CDS             4900..>5307
                     /locus_tag="PLN02_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOI51163.1"
                     /translation="MKSLCVFCGANAGNKPVYRATAENLGRLLAGRGIQLIYGGGNVG
                     LMGAVADACLDAGGTVIGVIPQALMGKEVEGRPVDHRTLTRLEVVDSMHTRKARMAEL
                     AEGFIAMPGGFGTFEEYCEILTWAQLGFHVKPIG"
BASE COUNT          971 a         1821 c         1578 g          937 t
ORIGIN      
        1 cggcacctgg attgccgggc gagtgatcgc ccaggaactc agtgccccgc tcgccgtcgc
       61 cgcgctgcgc ttcgtcgtcg ccggcctggt ggtcggcggc gtcatgctcg tctccggcgg
      121 cggcatcgcg ctgcccaagg accggcagga atggggactg gtctgggccc tcggtttctt
      181 tggcatcttc ctttacggcc tgtgcttctt tttcgggctg cagcacattc cggccgggcg
      241 cggcgccctc gttgtcgcgc tcaacccggt ggtcatcgtg ctgctcgcct gggccatcgg
      301 caaggaaggc atgaaccggc gcaaggcgct gggcagcgca atcgcgctcg ccggctgcct
      361 gacggtgatc ggcaacggcg acccgctggc cctgctgcaa ggcacggtcg gcatcggcga
      421 gtggctgatc gtcggctgcg tgctgagctg ggcggcctac accttcatcg gctggcgggc
      481 gaccggccat ttctcggcgc tggcgaccac gctctacgcc agcttcagcg gcgccttcct
      541 gctcggcctg gccgcgctgg tccagggcga cgtcgatttc gccggctggt cgtggcgggt
      601 gtggagcagc atgctcttcc tcgccgtctt cggcaccgcc atcgcctaca cctggttcac
      661 cgccgccgtg cagcaactcg gcgccggcca cgcctcggtc ttcatcaatc tggtaccggt
      721 cttcgccgtg ctgcaagcgg ccgtcctgct cgacgagcgg ctcggcctgc cggtgctttt
      781 cggcggcctg ctggtgattg ccggcgtctg gctgaccacc gtacaaaaaa caactctgga
      841 gaaaacagca tgattctgac gcaagaacag gaaatgatcc gcgacaccat gcgcgccttc
      901 gcgcaggaac gcctcgcccc cttcgccgcc gaatgggaca agaaccatac cttcccggcc
      961 gacgccctga aggaactcgg cgaactcggc gccatgggca tgtgcgtgcc cgaggaatgg
     1021 gacggcgccg gcatggacta catgagcctg gtgctgacgc tggaagaaat cgccgccggc
     1081 gacggcgcca cctcgaccat cgtctcggtg cagaactcgc tggcctgcgg catcacgcag
     1141 aaatacggta ccgacgcgca aaaggaagaa tggctgaagc cgctcgcccg cggcgaaaag
     1201 tggggctgct tctgcctgac cgaaccgcac accggctccg acgccgccgc catcaccacg
     1261 cgcgccgacc gtgacggcga ccatttcgtg ctcaacggcg tcaagcagtt catcaccacc
     1321 ggcaagcacg cccacatggc catcgtcttt gcggtgaccg acaaggcggc cggcaagaag
     1381 ggcatttcct gcttcctgat cccgaccagc acgcccggct tcgtcgtcgg ccgcaccgag
     1441 gacaagatgg gccagcacgc ctccgacacc gtgcagatca tcctggaaaa ctgccgcgtc
     1501 ccggcttccg cgctgctcgg caaggaaggc gaaggctaca agatcgccct ctccaacctc
     1561 gaagccggcc gcatcggcat cgccgcccag agtatcggca tggcgcgcgc cgcgttcgaa
     1621 gccgccgtgc gctacgccaa ggagcgcgtc accttcggcg tgccgatcat cgaccaccag
     1681 gcggtcaact tcaagctggc cgacatgaac accctgctcg acgccgcccg cctgatggtc
     1741 tggcgcgccg cccagctcaa ggacgccggc aagccctgcc tgaaggaagc gtccatggcc
     1801 aagatgttcg cctccgaagc cgccgagaag atcgcctccg acgccatcca gatccacggc
     1861 ggcgttggct acaccagcga cttcccggtc gaacgcatct accgcgatgt gcgcatctgc
     1921 cagatctacg aaggcgccaa cgacatccag cggctggtga tcggtcgttc gatcgccgcc
     1981 gagtaaggaa agaaatggcc tcgccaaaat caaacagccg tcccgacgtc gaagagcact
     2041 cccttcggct ggcagtactg atcgatgccg ataatgcgca ggcctcggtc attgagggct
     2101 tgttggcgga aattgcccgt tttggcgagg ccagcgtcaa acgtatctat ggcgatttca
     2161 cgtcgcccaa tagcgcgcag tggaaaaaag tgctcaatca gcacgccatc aaaccggtgc
     2221 agcagtttgc ttacaccacc ggcaaaaatg cgaccgatgg gacaatgatc atcgatgcga
     2281 tggatcttct ctacacccgt cgttttgatg gtttctgtct ggtttccagc gacagcgact
     2341 tcaccggact ggccctgcgc atccgcgaag aaggcctgag cgtctttggt ttcggcgaag
     2401 aaaaaacacc ggatgcattc cgtaatgcgt gccacaagtt cgtttttacc gaggttcttc
     2461 gggcgctacc gatgacaacg ccccctgctg agcccgaatc agacgaacag cccatccagc
     2521 agacaagctc ctcccctgtc gccaagaaag ccctgccgct tcgctttgtt ttgcaagcac
     2581 tggatcaggc ctgtgatgac aggggctggg caaatctggg cgcttttggc agctatctga
     2641 cgaaactgca gtctgatttc gatccgcgtt tgtacggatt caagaaactg agcgaactgg
     2701 tcagatcaaa accccaccta tttgttgttg aagagcggca gctcaccggc agcaactcgc
     2761 cagtcattta tgtccgaaaa aggccgggaa agggaattca atgaccaggc caggccgaaa
     2821 ccagtcccag gtgttcaacg aaaggatcga aatcccggtc gctgtacagg agtgcatagc
     2881 cgtgttcgat gcaactggtc gcgatcaggg tgtcgatggt cttgcgcaca gtgatgcccc
     2941 gcgcccgcag ttggcggaag ttactcgccg cctgctgggc aatttcggcc cccaccagag
     3001 gcaccaccgg cagcgctgtc agcagcgccc tggcctgggc gtaatcccgg tcgctgacaa
     3061 acccctgcag gacttcggcc aggatcaggt cgccaacgac gatcaactga ctaccgagca
     3121 gtcggtccag gcagtcggtt tgtggcgtga cagcaccccg aaagtaatcg atccagacgc
     3181 tggaatcgac aagggtcacc gatcctgcct catgcgatca aggtcaccct gccagtcgag
     3241 gcgcccacga aacttgcgga tttcctcctg ctgggcaaga cgcaacaagg tcttcagccc
     3301 ggcctcaacc gcctcgcgct tggttttcaa accggtaagc tgcattgcgg cctgcatcag
     3361 gccgtcgtca ataacgatgt tggtacgcac gatacacacc ctttagcaaa cacatacaca
     3421 tcctagtttt tttccagaca attacaagca ggaggaaaca cggcatgact caggacaata
     3481 ttaacccgat cgatttctgg ttcgattttt ccagccccta cggctacctg atggccgaga
     3541 agatcgacga cgtcgccgcc cgcttcgggc gcaaggtgcg ctggcatccg atcctgctcg
     3601 gcgtcatctt ccaggccacc ggctcgcgtc cgccggccga cggcgtcagc aacaagggcc
     3661 gctacatgct gcacgacttc gcccgctcgg cgcgttttct gggcgttccc tacaagatgc
     3721 ccagccgctt tccgctgccg acgcagaacg ccgcccgtgc ctactactgg ctgcatggcc
     3781 aggattgcgc gctggcccga cgctttgccc gcgccgtcta tcgcggcttc ttcgtcgacg
     3841 acctcgacat ctccgcgccc gacaccgtgc tcgacatcgc cgccggcctc ggcatcgact
     3901 gcccagccct cgccgccgcg ctgcaggccc cggaaatcaa ggcccgcctc aaggacgaat
     3961 gcgagcaggc gctggctgcc ggcgtcttcg gctcgccgca cgtgatcatc gacggcgaag
     4021 ccttcttcgg cgccgaccgc ctgccgcaga tcgagcgctg gctggaaagc ggcggcttct
     4081 gagcatgtcg ctgcgcctgg tcctctacgc cagcctgctg gctattgccg gctggtcgct
     4141 ggccatcgag ccgggccagg tgcagcgccg cgacctgacg ctgagcaccg ccaggctcaa
     4201 acagcccttg accatcgcca tggccagcga cctccacgtc ggcgccccgc atgtcagccg
     4261 cgaagccctg cgtcgtctgg tcgggcgcat caacgaggca aaccccgacc tgatcctgct
     4321 gccgggcgat ttcatgatcc agggcgtcct cggcggaacc caggtcgccc cggaagatct
     4381 cgccgccgac ctggccggcc tgcatgcccg gcttggcgtt ttcgcaacgc tcggcaacca
     4441 cgactggcgt ttcgatggac cgcgcgtgcg gcgggcgctg gaacaagtcg gtatccgcgt
     4501 gctggaaaac gaagcgatcg agctgcccga aagcggactc tggctggccg gtatcggcga
     4561 cgacatgacc tgccatgccg aaccggcggc cgcctttgcc ggcgtgccga acggcgccga
     4621 cgtcatcgcc atgctgcacg atccggccaa cgccccagac ctgccgacct cgacccgcgt
     4681 cgccctggcc ggccacacac acggcggaca agtccgcctg cccttcgtcg gcgcgctgat
     4741 cgtccccggt cgctccgccc gggaacacgc ttacggctgg attccgggaa gcgccgtgcc
     4801 gacctgggtg agttcgggcg tcggcaccag cattctgccc atccgtttca actgcccacc
     4861 ggaattcgtc gtcctgcggc tcgccaacaa ggaaagctca tgaaaagcct ctgtgtcttc
     4921 tgcggcgcca acgccggcaa caaacccgtc tatcgcgcca cggccgagaa tctcggccgc
     4981 ctgctcgccg gccgcggtat ccagctcatc tacggcggcg gcaacgtcgg tctcatgggt
     5041 gccgtcgccg atgcctgtct cgacgccggc ggcacggtca tcggcgtcat cccgcaagcc
     5101 ttgatgggca aggaagtcga aggtcgcccg gtggaccacc ggacactgac ccggctcgaa
     5161 gtcgtcgatt cgatgcacac ccggaaggcg cggatggccg agctggccga gggtttcatc
     5221 gccatgcccg gcggcttcgg caccttcgag gaatactgcg aaatcctcac ctgggcgcaa
     5281 ctcggcttcc atgtgaaacc gatcggc
//
LOCUS       DAOSDX010000063         5279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5559_length_5279_cov_2.575995, whole genome shotgun sequence.
ACCESSION   DAOSDX010000063 DAOSDX010000000
VERSION     DAOSDX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5559_length_5279_cov_2.575995"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1564
                     /locus_tag="PLN02_02210"
     CDS             407..1564
                     /locus_tag="PLN02_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOI51164.1"
                     /translation="MANYEMRKFVVPEFIFGAGARHRVGDYARHFSARRVLIVSDPGV
                     IAAGWLKEVQDDLEKVGIPWVVFQALTPNPKDHEVMAGALLYAREHCDVIVAVGGGSV
                     VDCAKGIGIVHSNRCDITAFEGVDRVNIPGPPLICIPTTAGAAADVSQFCIICNTPGR
                     YKMAIISKSMVPDVSLVDPETTLTMNPYLTACTGLDALTHAIEAYVSTASSPIVDLHA
                     LAAIKLLWHNIETAVSQPTLMPARENMVLGSLQAGIAFSNASLGAVHALAHPLGGYLD
                     LPHGECNALLLEHVIRFNAATAPERYQQIAEAMGIDPRGLSPREMVGRITDAILARRT
                     RLGVLGSLETRGVRAADIPELASHAVKDACLVTNPRRATINDIKVIYGEAL"
     gene            1551..3593
                     /locus_tag="PLN02_02215"
     CDS             1551..3593
                     /locus_tag="PLN02_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51165.1"
                     /translation="MKPSDVNDDEATLQDMIVGLSGRSFRKSYYPQLRQNLEHLERFR
                     TLLDHTSDFVILIALPEDSVADANAALGQLLGRPVDTLVGQPFLSLGLTDAENVLAVL
                     HREMESTGGDGESPAYSQVVAFSSTQGAVWLEISYRLALLGERNYGVLVGRDITDRRR
                     NEQMLAEALAEKAALLDNAMVGIGLLHNRRIASCNRRFEEIFGYPPGGMLDLCTRQLY
                     PSEASFQMLGDEAYRQLAHGENFSHTLPLIKADGSSFWAEITGHSLNPAAPQESSIWI
                     VNDITEKKRAHELSVAKEAAEAANQAKSAFLASMSHEIRTPLNAITGMVHLVMRSGVT
                     PEQKDRLEKIDAASKHLLEIINAILDLSKIEAGKFTLETVPVKLNEIFSSVATMIAER
                     AHLKGLSLLTEIHPVPDNLLGDPTRLRQALLNYANNAVKFTDHGSVTLRASLLENTDD
                     HVLVRFEVSDTGHGIPPSALPRLFSSFEQADNSITRKYGGTGLGLSITKSIAKLMGGE
                     VGVSSEPEQGSTFWFTARLLKDSRKPPASDRHAGIDSEAILKSTYAGARILLVEDDPI
                     NREVAQIMLGDVGLRIDQAEDGTEAVRLAEKNDYALILMDMQMPNMDGVTATRILRQA
                     QRMKEVPIIAMTANVFAEDKRNCLAAGMNDFLAKPVDPDDLYDKLLIWLARTANRG"
     gene            3688..4908
                     /gene="sbcD"
                     /locus_tag="PLN02_02220"
     CDS             3688..4908
                     /gene="sbcD"
                     /locus_tag="PLN02_02220"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOI51166.1"
                     /translation="MRLLHTSDWHLGQHFMGKSRQAEHQALIVWLLEQVAEHGVDAVL
                     IVGDIFDTGTPPSYARELYSQLVVRLHEAGVALLLLGGNHDSPATLGESRELLARLGT
                     TVIAATHDDVRSQVVVLAQRDGTPGCIVSAVPFVRPRDVLQSQAGQSAEDKQLSLQQA
                     IQQHYDAVDAAARARMAELGVTLPVVATGHLTTVGASASESVREIYVGALEAFPTAAF
                     PPADYIALGHIHRPQKVGGLEHIRYCGSPIPLGFDEAKQQKEMLLVDFSADGLQAVTP
                     LPVPRFQALVQISGKLVDLSAQIGAAAALGTPERPTWLEVTVAEDDYLADLPARIEKM
                     TDGWPVEVLRIRRQRGNAAARLASVASETLDELDPHEVFARRLQQEELDAELQQALCE
                     RYRSVVAGLHGEEA"
     gene            4905..>5279
                     /locus_tag="PLN02_02225"
     CDS             4905..>5279
                     /locus_tag="PLN02_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI51167.1"
                     /translation="MKILSLRLKNLNSLKGEWTIDFTRPPFADNALFAITGPTGAGKS
                     TLLDAICLALYHQTPRLKTISAADNDIMTRHTADCLAEVEFEVKGAVYRAFWSQRRAR
                     DKADGALQAPKVELAAGDGTILS"
BASE COUNT         1043 a         1866 c         1575 g          795 t
ORIGIN      
        1 gaggaaaaca gcgccgccgc gcactcgacc gccagcactt ccgacgagat gctgcaactg
       61 gccgaccaga tgcgccagat cgtcgcccag tacaaggtct gaacgactct cccacgccag
      121 accgggccgg ccctgcgccg gcctttttca tgggcaggag gcgaagggcc cagcgacagg
      181 cgcggatcgg atcagcgccc gcagacaata caactcgcga catgcgtttg acccatcgca
      241 cttgccaggc ccggattcag gaaagcaacg ccttgcgcgc aagtggggca atcagggaaa
      301 gaagcaacac cgataccgcg tagctgcgcc ataatgaaac aaacaattcg cgcccaagcg
      361 cagcgattca ggccgggcaa tccagcaaag aggagatgca gaagccatgg caaattatga
      421 aatgcgcaag ttcgtcgtcc ccgaattcat cttcggcgcc ggcgcccggc accgggtcgg
      481 cgattacgcc cggcacttca gtgcccgccg ggtattgatc gtctccgacc ccggcgtgat
      541 cgccgctggc tggctgaagg aggtacagga cgacctggaa aaagtcggca ttccgtgggt
      601 ggtcttccag gccctgacgc ccaaccccaa ggatcacgaa gtgatggcgg gcgccctgct
      661 ctatgcccgc gaacactgcg acgtcatcgt cgccgtcggc ggcggcagcg tcgtcgactg
      721 cgccaaggga atcggcatcg tccattcgaa tcggtgcgac atcaccgcct tcgagggcgt
      781 cgaccgggtc aacattcccg gcccgccgct gatctgcatc ccgaccacgg ccggtgccgc
      841 cgccgacgtc tcgcagttct gcatcatctg caacacgccg ggccgctaca agatggccat
      901 catcagcaag agcatggtgc ccgacgtttc gctggtcgat cccgaaacca cgctgaccat
      961 gaatccctac ctgaccgcct gcaccggtct ggatgccctg acccatgcca tcgaggccta
     1021 cgtatcaacc gccagttcgc cgattgtcga tctgcatgcg ctggccgcca tcaaactgct
     1081 ctggcacaac atcgagaccg ccgtcagcca gccaacgctg atgccggccc gcgaaaacat
     1141 ggtgctgggc agcctgcagg ccggcattgc cttttccaac gccagcctcg gcgcggtaca
     1201 cgccctcgcc catccgctgg gcggttatct cgatctgccg cacggcgaat gcaacgcgct
     1261 gctgctcgaa cacgtgatcc gtttcaatgc ggcaacagca ccggagcgct accagcagat
     1321 tgccgaagcc atgggcatcg acccgcgggg cctgagcccg cgcgaaatgg tcggccgcat
     1381 caccgatgcc atcctcgcca ggcgtacccg gctgggcgtc ctcggcagcc tcgaaacgcg
     1441 cggcgtgcgc gctgccgaca tccccgaact ggccagccac gccgtcaagg atgcctgcct
     1501 ggtgaccaac ccgcggcggg cgacgatcaa cgacatcaag gtgatctatg gtgaagccct
     1561 ctgacgtgaa cgatgacgag gcaacgctcc aggacatgat cgtcggcttg agcgggcgtt
     1621 ctttccgcaa gagctactac ccgcaactca ggcagaatct cgagcacctg gaacgcttcc
     1681 ggacgctgct cgaccacact tcggatttcg tcatcctgat cgccctgccc gaggacagcg
     1741 tcgccgatgc caatgccgcg ctcggccagt tgctcggtcg gccggtcgac accctggtcg
     1801 gccagccttt cctgtcgctg ggcctgaccg atgccgaaaa cgtccttgcc gtactgcacc
     1861 gcgagatgga aagcaccggt ggcgacggcg aatcgccggc ctattcccag gtcgtcgcct
     1921 tcagttcgac ccagggcgca gtctggctgg aaatttctta tcggctggcc ttgctcggcg
     1981 aacgcaatta cggtgtcctg gtcggccgcg acatcaccga tcgccgccgc aacgagcaga
     2041 tgctggccga agcgctggcg gaaaaagcgg cgctgctcga caacgcgatg gtcggcatcg
     2101 ggctcctgca caatcggcgg atcgccagtt gcaaccgtcg tttcgaggaa atcttcggct
     2161 atcccccggg gggtatgctc gacctgtgca cccgtcagct ctatccctcg gaagccagct
     2221 tccagatgct gggcgacgaa gcctaccggc aactggcgca cggtgagaac ttcagccaca
     2281 cgctgcccct gatcaaggcc gacggcagca gtttctgggc cgagatcacc ggccattcgc
     2341 tgaatccggc ggcgccgcaa gaaagcagca tctggatcgt caacgacatc accgagaaga
     2401 aacgggcaca cgaactgagc gttgccaagg aagccgccga agccgccaac caggcgaaaa
     2461 gcgctttcct ggccagcatg agccacgaaa tccgcacccc gctcaacgcc atcaccggca
     2521 tggtgcatct ggtgatgcgt tcgggcgtga cgccggagca gaaggatcgc ctggaaaaaa
     2581 tcgatgccgc cagcaaacac ctgctggaaa tcatcaacgc gatcctcgat ctgtcgaaga
     2641 tcgaagccgg caagttcacg ctggaaacgg taccggtcaa gctcaacgaa atcttcagca
     2701 gcgtcgccac gatgatcgcc gagcgcgccc atctcaaggg gctgagcctg ctcaccgaaa
     2761 tccatccggt gcccgacaac ctgctcggcg atccgacccg tttgcgccag gcgctgctca
     2821 actacgccaa caacgcggtc aaattcaccg accacggcag cgtcaccctg cgcgccagcc
     2881 tgctggaaaa taccgacgac cacgtcctgg tgcgcttcga agtcagcgac accggccacg
     2941 gcatcccgcc gtccgccctg ccccgcctgt tttccagctt cgaacaggcg gacaacagca
     3001 tcacccgcaa atatggcggc accggcctcg gactttccat caccaaaagc atcgccaaac
     3061 tgatgggcgg cgaggtcggc gtcagcagcg aaccggaaca gggcagcact ttctggttca
     3121 ccgcccgcct gctcaaggac agccgcaaac cgccggccag cgaccggcat gcgggcatcg
     3181 acagcgaagc gatcctgaaa tcgacttacg ccggcgcccg catccttctc gtcgaagatg
     3241 atccgatcaa ccgcgaagtc gcccagatca tgctgggcga cgtcggcctg cgcatcgacc
     3301 aggccgagga cggcaccgaa gccgtgcggc tggccgaaaa gaacgactac gcgctgatcc
     3361 tgatggacat gcagatgccc aacatggacg gcgtcacggc aacgcgcatc ctcaggcagg
     3421 cgcagcggat gaaggaagtg ccgatcatcg ccatgaccgc caatgttttc gccgaagaca
     3481 aacggaactg cctcgctgcc ggcatgaacg acttccttgc caagccagtc gatcccgacg
     3541 atctctatga caagctgctg atctggctgg cgcgcaccgc caaccgcggc tagtcgccaa
     3601 ggccttgccg actgccccat gggcccgcgc cgcgtccgtg ttgacgacgg caacgcttag
     3661 aatgccatct ttttcccctt cccccacatg cgcctcctcc acacctccga ctggcacctc
     3721 ggccagcatt tcatgggcaa gagccggcag gccgagcacc aggcgctgat cgtctggttg
     3781 ctcgaacagg tggcggaaca cggcgtcgac gcggtgctga tcgtcggcga catcttcgat
     3841 accggcacgc caccgagtta cgcccgcgag ctgtacagcc agctggtcgt gcgcctgcac
     3901 gaagccggcg tcgccctgct gctgctcggc ggcaaccacg attcgccggc gacgctgggc
     3961 gaaagccgcg aactgctggc gcgcctgggg acgacggtga tcgccgcaac gcacgatgac
     4021 gtgcgcagcc aggtggtcgt cctggcgcag cgcgacggca cgcccggctg catcgtcagc
     4081 gccgtgccct tcgtccgccc gcgcgacgtg ctgcagagcc aggccgggca gagcgccgaa
     4141 gacaagcagt tgtcgctgca gcaggcgatc cagcagcact acgacgcggt cgacgccgcc
     4201 gcccgggcca gaatggccga gctcggcgtc accctgcccg tcgtcgccac cggccacctg
     4261 accacggtcg gcgccagcgc cagcgagtcg gtgcgcgaaa tctacgtcgg cgcgcttgaa
     4321 gcgttcccca ccgccgcctt cccgccggcc gactacatcg cgctcggcca catccaccgg
     4381 ccgcagaagg tcggcgggct ggagcacatc cgctactgcg gctcgccgat tccgctcggc
     4441 ttcgacgaag cgaaacagca gaaggaaatg ctgctcgtcg acttcagcgc cgacggcctg
     4501 caggcggtga caccgctgcc ggtaccgcgc ttccaggcgc tggtacagat ttccggcaag
     4561 ctcgttgacc tgtcggcgca gatcggcgcc gccgccgcgc tgggcacgcc ggaacggccg
     4621 acctggctgg aagtcaccgt cgccgaggac gactacctcg ccgacctgcc ggcgcgcatc
     4681 gaaaagatga ccgacggctg gccggtcgaa gtcctgcgca tccgccgcca gcgcggcaac
     4741 gccgccgccc gcctggcatc cgtcgccagc gagacgctgg acgaactcga tccgcacgaa
     4801 gtcttcgccc gccgcctgca gcaggaggaa ctcgacgccg aattgcagca ggcgctttgc
     4861 gagcgctacc gcagcgtcgt cgccggcctg cacggggagg aagcatgaaa atcctgagcc
     4921 tgcgcctgaa gaacctcaat tcgctgaaag gcgaatggac catcgacttc acccggccgc
     4981 cgttcgccga caacgcgctg ttcgcgatca ccggaccgac cggcgccggc aagtccacgc
     5041 tgctcgacgc catctgcctg gcgctctacc accagacgcc gcgcctgaag acgatctcgg
     5101 ccgccgacaa cgacatcatg acgcggcaca ccgccgactg cctggccgag gtcgaattcg
     5161 aggtcaaggg cgccgtgtac cgcgccttct ggagccagcg ccgcgcccgc gacaaggccg
     5221 acggcgcgct gcaggcgccg aaagtcgaac tcgcggccgg cgacggcacc atcctcagc
//
LOCUS       DAOSDX010000064         5271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5571_length_5271_cov_3.621549, whole genome shotgun sequence.
ACCESSION   DAOSDX010000064 DAOSDX010000000
VERSION     DAOSDX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5571_length_5271_cov_3.621549"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLN02_02230"
     CDS             complement(<1..758)
                     /locus_tag="PLN02_02230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI51168.1"
                     /translation="MKLNLRTLTAADANEIERVRQFFRNYAGWLGVDLSFQNFDQEMA
                     SLPGAYAPPHGRLFFAEYDGQGAGCVGIRPLADATGVCEMKRLYVEPNFRGLGIGRRL
                     ALAAIKAAKELGYRKLLIDTLPSMRMAVRLYRELGFTEAPSYYHTPIEGTIFLALDLD
                     NWSEEEIRSENLCHLFDFNRAWARQMQEVDPQYFDKLSTLQAPEFMWIGCSDSRVPAN
                     QIVGLLPGEVFVHRNIANIVVHTDLNALSVIHYAV"
     gene            complement(890..2062)
                     /locus_tag="PLN02_02235"
     CDS             complement(890..2062)
                     /locus_tag="PLN02_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HOI51169.1"
                     /translation="MNIPSLDFALGEDINLLRDAVKAFADAEIAPRAAEIDRANEFPA
                     DLWQKFGDMGLLGMTAGEEYGGTNLGYLAHIVALEEISRASASVGLSYGAHSNLCVNQ
                     IRRNGSEAQKQKYLPKLISGEHVGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGAKM
                     WITNGGDADTLVVYAKTDPEAGAKGMTAFIVEKGMPGFSHGTHLDKLGMRGSNTFPLF
                     FDDCEVPEENVLGGVGNGTRVLMSGLDYERAVLCGGPLGIMAACMDVVVPYLHERKQF
                     GQAIGEFQLMQGKVADMYSTWQATRAYVYAVGRACDSSDHSRTLRKDAAGAILYSAEK
                     ATWMAGEAIQTLGGVGYTNEYPTGRLWRDAKLYEIGAGTSEIRRLLTGRELFNETT"
     gene            complement(2059..2535)
                     /locus_tag="PLN02_02240"
     CDS             complement(2059..2535)
                     /locus_tag="PLN02_02240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOI51170.1"
                     /translation="MAPPTDPGFAARVLASFARQSAMHLIRATMPVVDYGCTEIHVPH
                     WDGIEQQHGFVHGGVVGMIADSAAGYAAMTTVPASASVLTVEYKMNLLAPADGDKLIA
                     RGKLVRAGRTLIVTQAEVFAVRDGRENLCALMQQTIMAMHGKSDSKTSNNKPETNQ"
     gene            complement(2597..2986)
                     /locus_tag="PLN02_02245"
     CDS             complement(2597..2986)
                     /locus_tag="PLN02_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOI51171.1"
                     /translation="MSQPATFSISDLAREFAITPRTIRFWEDQGILAPQREGQKRIFT
                     RRDRARLKMALRGKRLGLSLAEIKDLIGMYNSTEDETPQLLECLRIIEKRRAALEQQR
                     EDIEAVLAEIGQFEQQCQQEVARRKNA"
     gene            3084..4124
                     /locus_tag="PLN02_02250"
     CDS             3084..4124
                     /locus_tag="PLN02_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI51172.1"
                     /translation="MSLHYPFTEIPAAGEQREVAPGIFWLRMPLPFQLDHINLWLLRD
                     GEGWTIVDTGFPDDGVRATWRSILDRLDGPVKRLIVTHFHPDHLGLATWLMEQTGAPL
                     AMTSAEFLTAHAVWNEVGGHGAQYMVEQFRRHGLDADRLDKFASRGSGYRRAVPGLPA
                     HYQRLKAGEPVTIDGQAWKILIGYGHSPEHLALYCAKLGVLISGDMLLPKISTNISVF
                     AATPDADALRWYLESLTVMAEEIPPQTLVLPSHGLPFTGVQARVGALHAHHEERLHAL
                     ENSCEQAPQSAAGLLDVLFGRALDTHQTMFAMGEAIAHLNYLEQAGRLSREIGDDGVI
                     RYSRLSKQSAAH"
     gene            4140..>5271
                     /locus_tag="PLN02_02255"
     CDS             4140..>5271
                     /locus_tag="PLN02_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOI51173.1"
                     /translation="MPQQTEEQGLNLPNPAEMAKTYAEVAQRASRIITQYMEKKAKDG
                     VEAPSDEMGVARAFMDLSARLMANPYKLAQTQMNMMWDYFSLWQGSMMKMLGMPGAAP
                     VAVPQKGDKRFKDEDWEQHFLFDFVKQSYLIAARHIHETVCCTDGLDEATQQKVNFFT
                     RQYIDALSPSNFALTNPEVFRETVKSHGQNLIKGLNNLLHDMNEDGQLRIRMTDTAAF
                     EMGRNVATTPGKVVYQNELFQLIQYNPGTTEQYKKPFLIVPPWINKYYILDLREKNSF
                     VKWATDQGHTTFIISWVNPDEKMAQVGFEDYMLNGTLEAISQVCAQTGEDSVNLAAYC
                     IGGTLTMSTLAYMAAKKDKRVNSATFFTTMLDFSEPGDLSVFL"
BASE COUNT          954 a         1592 c         1708 g         1017 t
ORIGIN      
        1 acggcgtagt ggatgaccga cagcgcgttg aggtcggtat ggacgacgat gttggcgatg
       61 ttgcgatgga cgaatacctc gcccggcagc aggccgacga tctggttggc cggcacccgc
      121 gagtcggagc agccgatcca catgaattcg ggcgcctgca gcgtcgacag cttgtcgaaa
      181 tactgcgggt cgacttcctg catctggcgc gcccaggcgc ggttgaagtc gaacaggtgg
      241 cagaggtttt cgctgcggat ttcctcttcc gaccagttgt cgaggtcgag ggcgagaaag
      301 atcgtgcctt cgatcggcgt gtggtagtag ctcggcgcct cggtgaaacc cagttcgcgg
      361 tagagcctga ccgccatgcg catgctgggc agggtatcga tcagcagctt gcggtagccg
      421 agttccttgg ccgccttgat cgcggccagt gccagtcggc ggccgatgcc gagaccacgg
      481 aagttcggct cgacgtagag gcgcttcatt tcgcagaccc ccgtcgcatc ggcgagcggc
      541 cggatgccga cgcaaccggc gccctgaccg tcgtattcgg cgaagaacag ccggccgtgc
      601 ggcggtgcgt aggcaccggg caacgaggcc atttcttggt cgaaattctg gaacgacagg
      661 tcgacgccca gccagcccgc gtagttgcgg aagaattggc ggacgcgctc gatttcgttg
      721 gcgtcggcgg cggtcagggt gcgtaggttc agtttcatgc ggtgtggctc ctgatagggc
      781 ggcgctgcga ggacgcgccg cgatgtgcgt ttacccagcc cgaggctgcg gtcaggcgca
      841 cattctatcg tgccgataat tctgaattca ggaagtcgcc agccatctgt caggtcgttt
      901 cgttgaacag ctcccggccg gtcagcaagc gacggatctc cgaagtcccg gcgccgatct
      961 cgtagagctt ggcgtcgcgc cagaggcggc cggtcgggta ttcgttggtg tagccgacgc
     1021 cgcccagtgt ctggatcgcc tcgccggcca tccaggttgc cttttcggcg gagtagagga
     1081 tggcgccggc agcgtccttg cgcagcgtcc ggctgtggtc gctgctgtcg caggcgcggc
     1141 cgacggcata gacataggcg cgggtggcct gccaggtcga gtacatgtcg gcgaccttgc
     1201 cctgcatcag ctggaactcg ccgatcgcct ggccgaactg cttgcgttcg tgcaggtagg
     1261 ggacgacgac gtccatgcag gcggccatga tgccgagcgg gccgccgcag agcacggcgc
     1321 gctcgtaatc gaggccggac atcagtaccc gggtgccgtt gccgacgccg ccgagcacgt
     1381 tctcctccgg tacttcgcag tcgtcgaaga acaacgggaa ggtgttggaa ccgcgcatgc
     1441 cgagcttgtc gaggtgggtg ccgtgggaaa agcccggcat gcctttctcg acgatgaagg
     1501 cggtcatgcc cttggcgccg gcctccggat cggtcttggc atagacgacc agggtatcgg
     1561 cgtcgccgcc gttggtgatc cacatcttgg cgccattgag cacgtagcgg tcgcccttct
     1621 tgtcggcgcg cagcttcatc gagacgacgt cggagccggc gttcggctcg gacatcgcca
     1681 gcgcgccgac gtgctcgccg gaaatcagct tgggcaggta tttctgcttc tgcgcttcgc
     1741 tgccgttgcg gcggatctgg ttcacgcaca ggttggagtg ggcgccgtag gagaggccga
     1801 ccgaggcgga ggcgcgcgag atttcctcaa gggcgacgat atgcgcgaga taaccgaggt
     1861 tggtgccgcc gtattcttcg ccggcggtca tgccgagcag gcccatgtcg ccgaatttct
     1921 gccacaggtc ggcggggaat tcgttggctc ggtcgatctc ggcggcgcgc ggcgcgatct
     1981 cggcgtcggc gaaggctttc accgcgtcgc gcagcaggtt gatgtcttcg ccgagggcga
     2041 agtcgaggct gggaatgttc attggtttgt ctccggtttg ttgttgcttg tcttgctatc
     2101 cgatttgccg tgcatcgcca tgatcgtctg ctgcatcaag gcgcacaggt tttccctgcc
     2161 gtccctgacg gcgaacacct cggcctgggt gacgatcagc gtgcgcccgg cgcgtactag
     2221 cttgccgcgg gcgatcagct tgtcgccgtc ggccggggcg agcaggttca tcttgtattc
     2281 gacggtgagc accgaggcgc tggccggcac cgtggtcatc gccgcgtagc cggcggccga
     2341 gtcggcgatc atgccgacga cgccgccatg gacgaaaccg tgctgctgct cgatgccgtc
     2401 ccagtgcgga acgtggattt cggtgcagcc gtaatcgacg accggcatcg tcgcgcggat
     2461 caggtgcatg gcgctttggc gcgcgaaact ggcgagaacg cgggcagcga atccgggatc
     2521 ggtcgggggg gccatggcgg tcagggtagt gaattaattt acgttgacgt aaacgtaaac
     2581 taaccggcaa aaaaaatcac gcatttttcc ggcgcgccac ttcctgctgg cactgctgct
     2641 cgaactggcc aatctcggcg agcaccgcct cgatgtcttc gcgctgctgt tccagtgcgg
     2701 cgcggcgctt ctcgatgatg cgcaggcatt cgagcagttg cggcgtttcg tcctcggtcg
     2761 agttgtacat gccgatcagg tccttgatct cggccagact caagccgagg cgcttgccgc
     2821 gcagcgccat cttcaggcgg gcgcggtcgc ggcgggtgaa gatgcgcttc tggccctcgc
     2881 gctgcggcgc caggatgccc tggtcttccc agaaacggat ggtgcgcggg gtgatcgcga
     2941 actcgcgcgc gaggtcggaa atgctgaagg tggccggctg gctcatcatg actccttggt
     3001 tgcggcgaag ttaagcagat ggcggggctt ttttcaatcc gctaccgtat ggtaacttgc
     3061 tcgaaaaacg aaaacggaaa accatgtccc tgcattatcc ctttacagag attcccgctg
     3121 ccggtgagca gcgtgaagtc gcgccaggca ttttctggct gcgcatgccg ctgcctttcc
     3181 agctcgacca tatcaacctc tggctgttgc gcgacggcga gggctggacc atcgttgata
     3241 ccggctttcc cgacgacggc gtgcgcgcca cctggcgcag catcctcgat cgccttgatg
     3301 gtccggtgaa gcgcttgatc gtcacccact tccatcccga tcacctcggt ctggcgacct
     3361 ggttgatgga acagaccggc gcgccgctgg cgatgaccag cgccgagttc ctgaccgcgc
     3421 acgcggtgtg gaacgaggtc ggcggccatg gcgcccagta catggtcgaa cagttccggc
     3481 ggcatggcct ggacgctgac cgcctggaca agttcgccag ccgtggctcg ggctaccggc
     3541 gggcagtgcc gggattgccg gcgcactatc agcgcttgaa ggcgggcgag ccagtaacga
     3601 tcgacggaca ggcctggaaa attctcatcg gctatggtca ttcgccggaa catctggcgc
     3661 tttactgtgc caaactgggc gtgttaattt caggcgacat gctgctgccg aaaatctcga
     3721 ccaacatcag cgtttttgcc gcgacgccgg atgccgatgc cctgcgctgg tatctcgaat
     3781 cgctgacggt gatggccgag gaaattccgc cgcagacatt ggtcctgcca tcgcacggtc
     3841 tgcccttcac cggggtgcag gcgcgtgtgg gtgccttgca tgcgcaccac gaagaacgat
     3901 tgcatgcgct ggagaatagc tgtgaacaag cgccacagag cgcagccggg ttgctcgacg
     3961 tgttgtttgg tcgggcgctc gatacacacc aaaccatgtt cgcgatgggt gaagcgattg
     4021 cccatctgaa ctatctggaa caggccggac gattgtcgag ggaaatcggc gacgacggcg
     4081 tgattcgcta ttcgcggttg tcaaaacaat ccgccgcgca ctgacagagg gagtttcaaa
     4141 tgccgcagca aaccgaagaa cagggcctca acctgcccaa cccggccgaa atggccaaga
     4201 cctatgccga agtcgcccag cgcgcctcgc gcatcatcac ccagtacatg gaaaagaagg
     4261 ccaaggacgg ggtcgaggca ccgtccgacg aaatgggtgt cgcccgcgcc ttcatggacc
     4321 tgtcggctcg cctgatggcc aatccctaca agctggcgca gacccagatg aacatgatgt
     4381 gggattactt ctcgctgtgg cagggctcga tgatgaagat gctcggcatg ccgggcgccg
     4441 caccggttgc agtgccgcag aagggcgaca agcgcttcaa ggacgaggac tgggaacagc
     4501 atttcctgtt cgatttcgtc aagcagtcct atttgatcgc cgcccgccac atccatgaga
     4561 cggtctgctg caccgacggc ctggacgaag cgacgcagca gaaggtgaac ttcttcaccc
     4621 gccagtacat cgacgcgctg tcgccgtcca atttcgccct gaccaacccg gaagtcttcc
     4681 gcgaaaccgt caagagccac ggccagaacc tgatcaaggg tctgaacaac ctgctgcacg
     4741 acatgaacga ggacggtcag ttgcgtatcc ggatgaccga caccgccgcc ttcgagatgg
     4801 gccggaacgt cgcgacgacg ccgggcaagg tggtctacca gaacgaactg ttccagttga
     4861 tccagtacaa cccgggcacc accgagcagt acaagaagcc cttcctgatc gtgccgccgt
     4921 ggatcaacaa gtactacatc ctcgatctgc gcgagaagaa ttccttcgtc aaatgggcga
     4981 ccgatcaggg tcacaccacc ttcatcatct cctgggtcaa tccagacgag aagatggccc
     5041 aggtcggctt tgaagattac atgctgaacg gtacgctgga agcgatcagc caggtctgcg
     5101 cgcagaccgg cgaggactcc gttaacctgg ccgcttactg catcggcggc acgctgacca
     5161 tgtccacgct cgcctacatg gcggcgaaga aggacaagcg cgtcaattcc gccaccttct
     5221 tcaccaccat gctcgatttc tccgagccgg gcgacctctc ggtcttcctc g
//
LOCUS       DAOSDX010000065         5259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5584_length_5259_cov_3.114527, whole genome shotgun sequence.
ACCESSION   DAOSDX010000065 DAOSDX010000000
VERSION     DAOSDX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5584_length_5259_cov_3.114527"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLN02_02260"
     CDS             complement(<1..237)
                     /locus_tag="PLN02_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOI51174.1"
                     /translation="MAIKHFLQFKDFTREEHQYVFERTRWIKDQFKSYQKYWPLTDRT
                     LVMIFEKASTRTRLSFEAGVQQLGGSAIYLNTRDS"
     gene            complement(242..1414)
                     /locus_tag="PLN02_02265"
     CDS             complement(242..1414)
                     /locus_tag="PLN02_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOI51175.1"
                     /translation="MSHVMNTYARLPVAFSHGKGSRVTDTEGREYLDALSGIAVNTLG
                     HAHPKLVAAIAEQAGRLIHTSNLYGVTGQEKLADKLCALSGMQEVFFCNSGCEANEAA
                     IKLARFYGHKRGIELPTIIVMEKAFHGRTMATLSATGNYKVQVGFEPLVGGFVRVPYG
                     DLDAIRAVAEHNRNIVAVMLEVIQGEGGIHLRDAAYYRGVREICDAHEWLMICDEVQC
                     GMGRTGTWFGYQQVGVQPDVATLAKGLASGVPIGACMAGGRAAGLFGPGNHGSTFGGN
                     PLACTAALTTLDCIEEEGLLANAERIGALIRQGFAEGLAGTSGVVDIRGHGLMIGVEL
                     DRPCGALVAQGLAAGLLINVTGDTVIRLLPPLNFSEADARELVARMLPLIKAFLAG"
     gene            1554..2330
                     /locus_tag="PLN02_02270"
     CDS             1554..2330
                     /locus_tag="PLN02_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOI51176.1"
                     /translation="MRIAIFNQKGGVGKTTTALNLGAALHRSGNAPLLIDLDPQGHLS
                     SIHGNIPLASDKSLFAFFQDQQPLQSLEVAWEHIGSLIPAHQQLIKVDSIFGKGPAVL
                     NKLRLGLDALDAEHPDRHCLIDCCPYIGVLALNAIFACDTLLIPVSTDYLSLQAAEHI
                     TRTLQVLEPVMKRRVNRLYLLTRFDRRRRMSEDVRQRLCERYGDDVLDVAISENVTVA
                     ESPSLQQDVFRHNASSTGAHDYRSLLAELAERGCLPITTS"
     gene            complement(2317..2532)
                     /locus_tag="PLN02_02275"
     CDS             complement(2317..2532)
                     /locus_tag="PLN02_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51177.1"
                     /translation="MNKFLFRIRTRNGVVVDHLSFYGRDEDEARRKVAQIYNHCEVLE
                     CTQRRVSMNGRLGTVNYESLALPLSLS"
     gene            complement(2643..3704)
                     /locus_tag="PLN02_02280"
     CDS             complement(2643..3704)
                     /locus_tag="PLN02_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51178.1"
                     /translation="MEPTPQANPVLRRAIDEADLLLAYAAENGIELDDGVLATIVGAR
                     QLPDVAADATAVAATTKFWEAYGSLARAVQPVSSDSIEATMSRQGRRGFAGRLLDRLF
                     GPKLTTLAERSIYRFSLASFVVLVLLLAVQMYWVIGNRLEAQAAQLVADAERVGERLA
                     TLNKAQLADDDRELLKLEAASEQLDLDLAIRVAALEQWNDIWRALVPGIARRSAALDE
                     TAGSPSPFSTMRKARLEASLAKECVELYLLPLLYGLLGSCLHVLRTLAAGIERRTHVA
                     GRLYQVRIYTGGLTGLVIAWFGVGGDATSPLASLTPFALAFLAGYSVELLFAFMDRLV
                     SAFVGRSEALPGPGGGAAK"
     gene            complement(3706..4245)
                     /locus_tag="PLN02_02285"
     CDS             complement(3706..4245)
                     /locus_tag="PLN02_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51179.1"
                     /translation="MKAANILAGILAALAASADAAQQEDTAGAAPPLVEIAEARWASA
                     VDPGSRQPAVLLNDARARRPLVLWMRIRASEAALARLEQAGRLPIRHRWFRDSFAGVQ
                     AEGQTEMTDSIALSAGSRELLAALRQEVSARGHFDWRTWSAKARPGPGRWSVAVVYAD
                     HTPVLCDGGKPCRYSITVK"
     gene            complement(4353..>5259)
                     /locus_tag="PLN02_02290"
     CDS             complement(4353..>5259)
                     /locus_tag="PLN02_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOI51180.1"
                     /translation="LIASLVWGWLALYITDLAPYVVDALEQVDFTIGKQHLNLWLIFS
                     GIVTVFLTVVFALWIAGVIEGRLMRMQSVDGNLRIVGVRVAKAVLTVVAVLISLSLVG
                     IDMTALSVFTGALGVGLGFGLQKIASNYVSGFIILLDRSIRIGNIVQVGTDSGTVTQI
                     TTRYTVLRHPGGTEFIVPNETLIGSTVQNQTYSDNSMRLFTTVGVAYDSDLELAMRLM
                     VECAQRQERVLADPAPSVLLTQFADSSINLELGFWVADPENGKMNVVSAINLDIWRCF
                     REHGVQIPFPQREVRLLGGAPQGLS"
BASE COUNT          973 a         1733 c         1689 g          864 t
ORIGIN      
        1 ggaatcgcgg gtattcaggt agatcgccga gccgccgagc tgctgcacgc cggcttcgaa
       61 cgacaggcgg gtgcgcgtgc tcgccttctc gaagatcatc accagcgtgc ggtcggtcag
      121 cggccagtac ttctggtagg acttgaactg gtccttgatc cagcgcgtgc gctcgaaaac
      181 gtactggtgt tcttcgcggg tgaaatcctt gaactgcagg aaatgcttga tcgccatcgt
      241 ctcaccccgc caggaaggcc ttgatcagcg gcagcatgcg ggccaccagt tcgcgggcgt
      301 cggcctcgct gaagttgagc ggcggcagca ggcggatcac ggtgtcgccg gtgacgttga
      361 tcagcaggcc ggcggccagc ccctgggcga ccagggcgcc gcacggccgg tcgagttcga
      421 cgccgatcat caggccgtgg ccgcggatgt cgacgacgcc cgaagtgccg gccaggcctt
      481 cggcgaagcc ctggcggatc agcgcgccga tgcgttcggc gttggccagc aagccttctt
      541 cctcgatgca gtcgagggtg gtcagcgccg ccgtgcaggc gagcgggttg ccgccgaagg
      601 tcgagccgtg gttacccggc ccgaacagcc cggcagcgcg gccgccggcc atgcaggcgc
      661 cgatcggcac gccggaagcg aggcccttgg cgagcgtcgc gacgtccggc tggacgccga
      721 cctgctggta gccgaaccac gtgccggtac ggcccatgcc gcactgcact tcgtcgcaga
      781 tcatcagcca ctcgtgggcg tcgcagatct cgcgcacgcc gcggtagtag gccgcgtcgc
      841 gcagatggat accgccctcg ccctggatca cctcaagcat caccgcgacg atgttgcggt
      901 tgtgctcggc gaccgcgcgg atggcgtcga gatcgccgta gggaactctg acgaagccgc
      961 cgaccagcgg ctcgaagccg acctgcacct tgtagttgcc ggtggccgac agggtcgcca
     1021 tggtccgccc gtggaaggcc ttttccatga cgatgatggt cggcaactcg atgcccctct
     1081 tgtggccgta gaaacgcgcc agcttgatcg ccgcctcgtt ggcttcgcaa ccggaattgc
     1141 agaagaacac ttcctgcatg ccggacagcg cgcacagctt gtcggccagt ttttcctggc
     1201 cggtgacgcc gtagaggttg gaagtgtgga tcaggcgtcc ggcctgttcg gcgatcgccg
     1261 cgaccagctt gggatgggcg tggcccaggg tgttgacggc gatcccggag agcgcgtcca
     1321 ggtactcgcg gccctcggta tcggtgaccc ggctaccctt gccgtgactg aaagccaccg
     1381 gcaggcgggc gtaggtattc atgacatgcg acatgaggca gaccccataa aacgaaaacg
     1441 gcggccatcg ccgccggaac gattgcaacg cggaccggcg aaaataaccg gaaagagttg
     1501 cagaaagctg aatgttaagt gaaaaatagc gccttgtata gaacggccac accatgcgaa
     1561 tcgccatatt caaccagaaa ggcggcgtcg gtaaaacgac caccgcgctc aacctcgggg
     1621 ccgccctgca tcgttcggga aacgccccac tgctcatcga tctcgatccg caaggccatc
     1681 tttccagtat tcacggcaac atcccgctcg cctccgacaa gagcctgttc gccttttttc
     1741 aggaccagca gccgctgcag tcgctcgaag tggcgtggga acacatcggc agcctgattc
     1801 cggcccacca gcagctgatc aaggtggact cgatcttcgg caaggggccc gccgtcctca
     1861 acaaactgcg tctcggcctc gacgcgctgg atgcggagca tcccgatcgc cactgcctga
     1921 tcgactgctg cccctacatc ggcgtcctgg cgctcaacgc catcttcgcc tgcgacacgc
     1981 tgctcatccc ggtatccacc gactacctgt cgctgcaggc cgccgagcac atcacgcgca
     2041 cgctgcaggt gctcgaaccg gtgatgaagc gccgcgtcaa ccggctctac ctgctgaccc
     2101 gtttcgaccg ccgccggcga atgagcgaag acgtgcgcca gcgcctgtgc gaacgctacg
     2161 gcgacgatgt cctcgatgtc gcgatttcgg aaaacgtgac cgtggccgaa agcccgtcgc
     2221 tacagcagga cgtcttccgt cacaacgcct cgagcaccgg cgcccacgac taccgcagcc
     2281 tgctcgccga actggccgaa cgcggctgcc tgccgatcac gacaagctga gcggcagcgc
     2341 cagactttcg tagttcaccg tccccagccg gccgttcatg ctgacccgcc gctgcgtgca
     2401 ttcgagcacc tcgcagtggt tgtagatctg cgccaccttg cggcgcgcct cgtcctcatc
     2461 acgcccgtaa aaggaaagat ggtcgacgac gacgccgttg cgcgtcctga tccggaagag
     2521 aaatttgttc atcgtcccag ccagcataaa aagtccagat agatcaatgt gctacgggga
     2581 gtatatgccg ttggtactaa caacacaatc catttacatt gtaaaaaact gcgaaccacg
     2641 acctacttcg cagcgccgcc accaggcccc ggcagggcct cgctgcgccc gacgaatgcc
     2701 gacaccagac ggtccatgaa agcgaacagc agttcaacgc tgtagccggc aaggaaagcc
     2761 agcgcgaacg gggtcagcga ggcgagcgga ctcgttgcgt cgccgccgac gccgaaccag
     2821 gcgatgacca gcccggtcag cccaccggta tagatacgca cctgatagag gcggccggcg
     2881 acgtgggtac ggcgctcgat gccggccgcc agcgtgcgca gcacgtgcag gcaactgccg
     2941 agcaggccgt agagcagcgg cagcaggtag agttcgacgc attctttggc cagactggcc
     3001 tccagccggg ccttgcgcat cgtcgagaaa ggactgggac tgccggcggt ctcgtcgagc
     3061 gccgcactgc ggcgcgcaat accgggcacc agcgcgcgcc agatgtcgtt ccactgttcg
     3121 agggcggcga ctcggatcgc cagatcgaga tcgagttgct cgctggccgc ctcgagcttc
     3181 agcagttccc ggtcgtcgtc ggcgagttgc gccttgttca gcgtcgccag ccgttcgccg
     3241 acccgctccg cgtcggcgac cagttgcgcc gcctgggctt cgagacggtt gccgatcacc
     3301 cagtacatct gcaccgccag caacaggacg agcacgacga aggaagcgag cgagaagcgg
     3361 tagatgctgc gctcggccag tgtcgtcagc ttggggccga acaggcggtc gagcagccgg
     3421 ccagcgaaac cacgccgccc ctggcggctc atcgtcgcct cgatactgtc cgagctgacc
     3481 ggctgcacgg cacgggccag actgccgtag gcctcccaga atttcgtcgt cgccgccacc
     3541 gccgtcgcat cggcagccac atccggcagt tgtcgggcgc cgacgatggt ggccagtacg
     3601 ccgtcgtcga gttcgatacc gttctcggcg gcgtaggcga gcaacaggtc ggcttcgtcg
     3661 atggcgcggc gcagtaccgg gttggcttgc ggggtcggct ccatgtcatt tcaccgtgat
     3721 cgagtagcga cagggcttgc cgccgtcgca caggaccggc gtatggtcgg catagacgac
     3781 ggcgacgctc cagcggccgg gaccggggcg cgccttggcg ctccaggtcc gccagtcgaa
     3841 atgaccgcgc gcgctgactt cctggcgcag cgcggcgagc agttcgcgac tgccggcgct
     3901 gagggcgatc gaatcggtca tctcggtctg cccctcggcc tgcacgccgg caaagctgtc
     3961 gcggaaccag cggtggcgga tcggcaggcg ccccgcctgt tcgaggcgag cgagcgccgc
     4021 ctcgctggcc cggatccgca tccacagtac cagcggtcgg cgcgcccgtg cgtcgttgag
     4081 gaggacggcc ggctgccgcg atccgggatc gaccgcggaa gcccaacgcg cttcggcgat
     4141 ttcaaccagt ggcggggcgg cgcccgcggt atcttcctgc tgcgccgcgt cggccgatgc
     4201 cgccagggca gccaggatac cggccagaat atttgctgcc ttcatcgatc gcccgccttt
     4261 ccacctgctt ccttgcatag ccaggcaaaa taggccgcgc caccagcaat gtcaacgaca
     4321 ttgcttcacg ccaaaagcat gaaccaagaa cttcaggaaa gcccctgcgg tgcgccgccg
     4381 agcagccgga cctcgcgctg cgggaaggga atctgcacgc cgtgttcgcg gaaacagcgc
     4441 cagatgtcga ggttgatcgc cgacaccacg ttcatcttgc cgttctccgg gtcggcgacc
     4501 cagaagccca actcgagatt gatgctgctg tcggcaaact gggtcagcag cacgctcggt
     4561 gccggatcgg cgagcaccct ttcctggcgc tgcgcgcact cgaccatcag gcgcatcgcc
     4621 agctcgagat cgctgtcgta ggcgacgcca accgtggtga acaaacgcat gctgttgtcg
     4681 gagtaggtct ggttctgtac cgtactgccg atcagggttt cgttgggaac gatgaattcg
     4741 gtaccgccgg gatggcgcag caccgtgtag cgggtcgtga tctgggtgac cgtgccgctg
     4801 tcggtaccga cctggacgat gttgccgatg cggatcgagc ggtcgagcag gatgatgaaa
     4861 ccggaaacgt agttgctggc gatcttctgc aggccgaagc cgaggccgac gccgagcgcc
     4921 ccggtgaata ccgagagcgc cgtcatgtcg atgccgacca aggacagact gatgagcacg
     4981 gcgaccacgg tgagcaccgc cttggcgacg cgcacgccga cgatgcgcag gttgccgtcg
     5041 accgactgca tgcgcatcag gcgcccttcg atgacgccgg cgatccacaa ggcgaagacg
     5101 acggtcagga agacggtgac gatgccgctg aaaatcagcc acaggttgag atgttgcttg
     5161 ccgatggtga aatcgacctg ctccagcgca tcgaccacat agggcgcgag atcggtgatg
     5221 tagagcgcca gccagcccca gaccaggctc gcgatcagc
//
LOCUS       DAOSDX010000066         5221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5635_length_5221_cov_2.915989, whole genome shotgun sequence.
ACCESSION   DAOSDX010000066 DAOSDX010000000
VERSION     DAOSDX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5635_length_5221_cov_2.915989"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLN02_02295"
     CDS             complement(<1..220)
                     /locus_tag="PLN02_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51181.1"
                     /translation="MNQASANDPLNFMRNLWSNMGFGLPGMVTPTFDLEELDKRIKDL
                     KAVEGWLRMNLSMLQMTIQGLEMQRSTVS"
     gene            317..979
                     /locus_tag="PLN02_02300"
     CDS             317..979
                     /locus_tag="PLN02_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI51182.1"
                     /translation="MLKLLVVEDHALVREGLVGLLSQVEDGVHVFESADFESALSVLD
                     SEGDFDLVLLDLALPGIDGFAGLDILRRRYPAMPVAVVSAFDDQPTVTRVMNLGASGF
                     IPKALNGERLLLAVRDVLAGNIVRPSGQMPARLDDAVPLPPSSVSVKPGEIGLTERQA
                     QVLCLMVRGLSNRDIADQLDLSEGTVKIHATAVFKALGVTSRTQALVTAARYGIDFSD
                     LF"
     gene            complement(983..2908)
                     /locus_tag="PLN02_02305"
     CDS             complement(983..2908)
                     /locus_tag="PLN02_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51183.1"
                     /translation="MAEHTARAFEAVDVLVKEVATDLSLNRADWPDWEPVKGWEYIAQ
                     RHSRAMPQLRDLSVFDALGNQRFISSYFPSPQINISDRPYFATLARGTPWTTYGPYVG
                     RNSDHYTYAIAHRLSGPGNSFAGAAFAAIEPAYMQDFCWANRLSDDFDAVLTNAKGEI
                     VASCRPSDLSPRSGILGQQAGVALFGGRAAGMIPETGIVEVSGLLISTTPVPGFSDLR
                     MVAMMPTDSLLANWRNRLFEIGTLGIMLSALLFFGAVLVRRQVRDLRAMAEALADSRD
                     LLEERIDQATAALAAEKDTAERANAAKSRFLAAASHDLRQPLHALSLFTTDLLRQAAA
                     GKLREVPRIAEQIAASTQTLGDMLNALLDISRLDIDGVRANLQPFPLAAVFQRLHDAF
                     FRQAEAHQLRLRFHTPRYFVYSDPQLLERMLGNLISNALRYTPAGGAIVVGARLQGDR
                     LRIEVRDSGIGIAREHRAAIFAEFYQVANVAREQDGGLGLGLSIVQRLAKGLGIEVSL
                     ASQIGNGTTFSLLVERAAEQATPSVRSNREASNGAVHFVGESEELAACRELIASWSYA
                     TSSGDRQSVERLRSDTIVVCDAAWVPDLDPGMALIVIGPSGGAPLPANAHVLAPPVRP
                     ARLRALLRASAPRGPIA"
     gene            complement(2911..3060)
                     /locus_tag="PLN02_02310"
     CDS             complement(2911..3060)
                     /locus_tag="PLN02_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51184.1"
                     /translation="MQLTRKPEKLALLVALLGTLTVLVIFLTDLVLSYQRDIDSGEPA
                     SSSSR"
     gene            complement(3067..3816)
                     /locus_tag="PLN02_02315"
     CDS             complement(3067..3816)
                     /locus_tag="PLN02_02315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI51185.1"
                     /translation="MSSTPLRQFALQGAAVILVLSLAWPYYGWAGGSMPWQQTSLLIG
                     AVALALATITRQAWWWRLIHALFMPLVWLTWTLNIDPGWFLLAFILLLLVYRGALSGQ
                     VPLYLSNRETVAALDELIGKEESQRFLDLGAGIGSTLIPLADRWPDRQFTGAENAPLT
                     WLIGRILGVGRPNLHWLWEDLWQMPLGEHDLVYAFLSPVPMPKLWEKARAEMKPGSLF
                     VSNSFPVPGVTPECIVEVACSPPRPLYCYRI"
     gene            complement(3836..>5221)
                     /locus_tag="PLN02_02320"
     CDS             complement(3836..>5221)
                     /locus_tag="PLN02_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOI51186.1"
                     /translation="SCLKGFCPSFVTIEGGTLKKPSPAVDSTNWPILPAPALPSTATP
                     WNLIITGVGGQGTITLGALIGMAAHLDGKGVSTLDMTGLAQKYGAVFSHLRIADRPED
                     IHAARIATGEAHAILGGDLVVSASSDALSKMLEGKTRAVVSSTDTPTAEFTRNRDWRF
                     PQAGLQKQLTDTLGADAIDFLDAQHLAERLLGDTLFAGALLLGYAWQKGLVPVSAASL
                     DRAIELNGTAVAANRQAFQWGRRAAVDRQKVESIAGPLQTQRQEMTLAELIAHRATHL
                     TAYQDAALAERYRQRVAAIRALGDEALTRTVAEQYARLLAPKDEYEVARLYTEGDFAQ
                     RLAETFQGGKLAFHLAPPGLHRPGPDGRPKKWRFGPWLLPALALLAKARRLRGSWLDP
                     LRNSGERKTDRALLADYEADLELILKHRPSSARNLAAWPAEVRGYGPVREAALAPARQ
                     KREQARAASMA"
BASE COUNT          829 a         1589 c         1906 g          897 t
ORIGIN      
        1 tgctcaccgt gctgcgttgc atttccaggc cctggatggt catctgcagc atcgacaggt
       61 tcatccgcag ccagccttcg accgccttga ggtccttgat ccgcttgtcg agttcttcca
      121 ggtcgaaggt tggggtgacc atgccgggca gaccgaaacc catgttgctc cagaggttgc
      181 gcatgaagtt gagcgggtcg ttggcagatg cttggttcat ttttgtctcc tccggggtca
      241 tctgatgcaa cgatcttaaa ccacttttat cgtgcaaaat gataaattag cgcctgctat
      301 gaaaaaggaa taaccgatgc tcaagctgtt ggtggttgaa gatcacgcat tggtccgcga
      361 gggactggtc ggcctgcttt cccaggtgga agacggtgtg cacgtattcg agagcgccga
      421 tttcgagagc gcgctgagcg tgctcgacag cgaaggcgat ttcgatctgg tcctgctcga
      481 tcttgccctg ccggggatcg acggcttcgc cgggctcgac atcctgcgcc gtcgctatcc
      541 ggcgatgccg gtggcggtgg tttcggcttt cgacgaccag ccgacggtga cccgggtgat
      601 gaacctcggc gcttccggct tcattcccaa ggcgctcaac ggtgaacgtc tgctgctggc
      661 ggtgcgcgac gtgctggccg gcaatatcgt ccgcccgagt ggccagatgc cggcgcgtct
      721 cgacgacgcg gtgccgctgc cgccctccag cgtcagcgtc aagcccggcg aaatcggcct
      781 gaccgagcgc caggcgcagg tgctgtgcct catggtgcgc ggtctgtcga accgcgacat
      841 cgccgatcag ctcgacctct ccgaaggtac ggtgaagatt cacgcgaccg cggtcttcaa
      901 ggcgctcggc gttaccagcc ggacccaggc gctggtcacg gcggcccgct acggcatcga
      961 tttcagcgac cttttctgag actcaggcaa tcggcccgcg cggcgcgctg gcgcgcaaca
     1021 gggcgcgcag gcgcgctggc cggaccggcg gcgccagcac gtgggcgttg gccggcaggg
     1081 gggcgccgcc gctcgggccg atgacgatca aggccattcc cggatcgaga tcgggaaccc
     1141 aggcggcgtc gcagacgacg atggtgtcgc tgcgcaagcg ctccaccgac tgccggtcgc
     1201 cgctgctggt ggcgtagctc cacgaggcga tcagttcgcg gcaggcggcc agttcttccg
     1261 attcgccgac gaaatggacg gcgccgttgc tggcttcgcg gttgctccgg acgctcggtg
     1321 tcgcctgttc cgccgctcgt tcgacgagca gcgagaaggt cgtcccattg ccgatctgcg
     1381 aggccagcga aacttcgata cccagcccct tggccagtcg ctggacgatg gagagaccga
     1441 ggccgaggcc gccgtcctgt tcgcgggcga cgttggcgac ctgatagaac tcggcgaaga
     1501 tcgccgcccg gtgttcgcgg gcgatgccga tgccgctgtc gcgtacctcg atgcgcaggc
     1561 ggtcgccctg aaggcgggca ccgaccacga tggcgccgcc ggccggcgtg tagcgcaggg
     1621 cgttggaaat caggttgccg agcatgcgtt cgaggagttg cgggtcgcta tagacgaaat
     1681 agcgcggcgt gtggaagcgc aggcgcaact ggtgggcttc ggcctgccgg aaaaaagcgt
     1741 cgtgcaggcg ctgaaaaacc gcggccagcg ggaagggctg cagattggca cggaccccgt
     1801 cgatgtcgag gcgggaaata tcgagcaggg cgttgagcat gtcgcccagg gtttgcgtcg
     1861 aggcggcaat ctgttcggca atgcgcggca cttcgcgcag tttgccagcg gcagcttggc
     1921 gcagcaggtc ggtggtgaac agcgaaagcg catgcagcgg ctgccggagg tcgtggctgg
     1981 cggcggcgag gaagcggctc ttggcggcgt tggcgcgttc ggcggtgtct ttttcggcag
     2041 ccagcgcggc ggtcgcctgg tcgatgcgct cttcgagcag gtcgcggctg tcggcgaggg
     2101 cttcggccat cgcccgcagg tcgcgcacct ggcggcggac caggacggcg ccgaagaaga
     2161 gcagggcgct gagcatgatg ccaagggtgc cgatttcgaa caggcggttg cgccagttgg
     2221 cgagcagcga atcggtcggc atcatcgcca ccatgcgcag atcggagaag ccggggacgg
     2281 gcgtcgtcga gatcagcagg ccgctgactt cgacgatgcc ggtttccggg atcatgccgg
     2341 cggcgcggcc gccgaacagg gcgaccccgg cttgctggcc gagaatgccg gagcgggggc
     2401 tgagatcgct gggccggcag gaggcgacga tttccccctt ggcattggtc agcaccgcgt
     2461 cgaagtcgtc ggaaagacgg ttggcccagc agaaatcctg catgtaggcc ggttcgattg
     2521 cggcgaaggc ggcgccggcg aagctgttgc ccgggccgct caggcggtgg gcaatggcgt
     2581 aggtgtagtg gtcggaattg cgcccgacat aggggccata agtggtccac ggtgtgccgc
     2641 gggcgagggt ggcgaaatag gggcggtcgc tgatgttgat ctgcgggctg ggaaaatagc
     2701 tggagatgaa gcgctggttg cccagggcgt cgaagacgct caggtcgcgt aactggggca
     2761 tcgcgcggga atggcgctgg gcgatgtact cccagccttt gaccggctcc cagtccggcc
     2821 agtcggcgcg attgagcgag agatctgttg ccacttcctt gaccaggacg tcgacggctt
     2881 cgaaggcgcg ggcggtgtgt tcggccatca tcaccgagaa ctgcttgagg cgggttcgcc
     2941 gctgtcgatg tcgcgctggt aggaaagcac caggtcggta aggaaaatga ccagcaccgt
     3001 cagcgtcccg agcagggcca ccagcagggc gagtttttcc ggcttgcgcg tcagctgcat
     3061 gcgggatcag atccggtagc agtacaacgg ccgcggcggc gagcaagcga cctcgacaat
     3121 gcactccggc gtcaccccgg gtaccgggaa actgttgctg acgaagaggc tgcccggttt
     3181 catttcggcc ctggcctttt cccacagctt gggcatcggc accggcgaca ggaaggcata
     3241 gaccagatcg tgttcgccga gcggcatctg ccacaggtcc tcccacagcc agtgcaggtt
     3301 gggccggccg acgccgagga tgcggccaat cagccaggtc aatggcgcgt tttcggcgcc
     3361 ggtgaattgg cgatccggcc agcggtcggc gagcgggatg agtgtgctgc cgatgccggc
     3421 gccgaggtcg aggaagcgtt ggctttcctc cttgccgatc aactcgtcga gggcggcgac
     3481 ggtctcgcgg ttggagaggt agagcggcac ctgcccggaa agcgcgccgc gatagaccag
     3541 cagcaacagg atgaaagcga gcaggaacca gccggggtcg atgttgagcg tccaggtcag
     3601 ccagaccagg ggcatgaaca gggcgtggat caggcgccac caccaggcct ggcgggtgat
     3661 cgtcgccagc gccagggcga cggcgccgat cagcaggctg gtctgctgcc agggcatgct
     3721 gccgccggcc cagccatagt agggccaggc cagcgagagg acgaggatga cggcggcgcc
     3781 ctgcagggcg aactggcgaa gcggggtcga tgacatcccg cgattctacc cagatttatg
     3841 ccatgctcgc cgcgcgggct tgctcccgtt tctgcctggc cggcgccagc gccgcctcgc
     3901 gcaccgggcc gtagccccgg acttcggctg gccaggcggc aaggttccgc gccgagcttg
     3961 ggcgatgctt gaggatcagc tcaaggtcgg cctcgtagtc ggcgagcagg gcgcgatccg
     4021 tttttcgctc gccgctgttg cgcaaggggt ccagccaact gccgcgcagc cggcgggcct
     4081 tggcgagcag cgcgagagcg ggcagtagcc aggggccgaa acgccatttc ttcggccgcc
     4141 cgtccggccc cgggcggtgc agtccgggcg gcgccagatg gaaggcaagc ttgccgccct
     4201 ggaaagtttc cgccaggcgc tgggcgaagt cgccctctgt atacagccgc gccacctcgt
     4261 attcgtcttt gggggcgagg aggcgggcgt actgctcggc caccgtgcgc gtcagcgcct
     4321 cgtcgccgag ggcgcggatg gcggcgacgc gctgcctgta gcgctcggcc agcgccgcgt
     4381 cctggtaggc agtgaggtgg gtggcgcggt gggcgatcag ttcggctagc gtcatttcct
     4441 ggcgctgcgt ctgcagcggc ccagcgatgc tttcgacttt ctgccggtcg accgccgcgc
     4501 gccggcccca ctggaaggcc tggcggttgg cggccaccgc cgtgccgttg agttcgatgg
     4561 cgcggtcgag cgacgctgcc gataccggca ccagcccctt ctgccaggcg tagccgagga
     4621 gcagggcgcc ggcgaacagg gtgtcgccga gcaggcgttc ggccaggtgc tgggcgtcga
     4681 ggaaatcgat ggcgtcggcg ccgagggtgt cggtcagctg cttctgcagg ccggcctgcg
     4741 ggaagcgcca gtcgcggttg cgggtaaatt cggcggtcgg cgtgtcggtg ctgctgacca
     4801 cggcgcgtgt cttgccttcg agcattttcg acaaggcgtc gctgctggcg ctgacgacca
     4861 ggtcgccgcc gagaatggcg tgcgcctcgc cggtggcgat gcgggcggcg tggatgtcct
     4921 cgggccggtc ggcgatgcgc aggtgcgaga agacggcgcc gtacttctgc gccagcccgg
     4981 tcatgtccag cgtcgacacg cccttgccgt cgaggtgggc ggccatgccg atgagcgcgc
     5041 cgagggtgat cgtgccctgg ccgccgacgc cggtgatgat caggttccag ggtgttgccg
     5101 tggacggcag cgcaggcgcc ggcaaaatcg gccagtttgt gctgtcgacc gcagggctcg
     5161 gttttttcag cgtaccgccc tcgatagtca cgaaactcgg acagaagccc ttcaggcatg
     5221 a
//
LOCUS       DAOSDX010000067         5216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5640_length_5216_cov_3.123813, whole genome shotgun sequence.
ACCESSION   DAOSDX010000067 DAOSDX010000000
VERSION     DAOSDX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_5216_cov_3.123813"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLN02_02325"
     CDS             complement(<1..1122)
                     /locus_tag="PLN02_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI51187.1"
                     /translation="MLRHLTLRDFVIVDRLELEFAAGFGALTGETGAGKSILIDALAL
                     ALGERGDAGVVRAGCDKAEIAATFDIAGLPVVTAWLDENGLDASEDLLLRRVVDANGR
                     SRAWINGSPATVQQLREVGEWLVDIHGQHAHQSLLRADAQRQLLDSHGGLLDLGREVA
                     AAWKTWRDAEQAWQAANDGVQALQREREQLEWQVGELAALAFTADEWETLEGEHRRLA
                     HAASLIEGAQFALAVLADDDAACERQIDQVATRLADLAAYDPALADVAGVLASMQAEL
                     ADAVSTLRRYADRVDLDPGRLADVEKRIEAILAQVRRHRVSPPELPALLAGWQKRLAE
                     LAAAADVDALRAASDAAQAAYRTLAQRLSAGRQQVAADMG"
     gene            complement(1122..2039)
                     /locus_tag="PLN02_02330"
     CDS             complement(1122..2039)
                     /locus_tag="PLN02_02330"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOI51188.1"
                     /translation="MQEFAPMNRLRHPRTIALVGKYHSPEIAESLRRLAEYLHERGVS
                     VFVERETAEHIGKRVDLARWVTCGFNDIGAHADAAIVLGGDGTMLNAARRLARYCVPL
                     VGVNQGRLGFMTDIARDDMLSCMDDLLDGRFQRENRMLLAAEVVRDGKELASNVALND
                     VVVDKGAIGRMIELELFIDGEFIYNLRSDGLIVSTPTGSTAYSLSAGGPILHPTLTGI
                     AMVPLCPHALTNRPVIVNDNADIELRVVRADEPRVHFDGQLTLDLQPGDSVRLYRSEH
                     TICFLHPPGYSYFAMLRQKLQWSERPKGS"
     gene            2088..3116
                     /gene="hrcA"
                     /locus_tag="PLN02_02335"
     CDS             2088..3116
                     /gene="hrcA"
                     /locus_tag="PLN02_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOI51189.1"
                     /translation="MLDDRSRTLLKTLVEHYIADGQPVGSRALSKFSGLELSPATIRN
                     VMADLEEAGFVASPHTSAGRIPTARGYRLFVDNLLTVQPLEAHQRGAIEDALHGLPSK
                     QIIANASQLLSSLTHFAGVVIAPRRTANRIRQIEFLSLSDKRILLIIVTADGDVQNRI
                     LNCERAYSPSELTSAANYLNQNFTGLDFEQIRQRLAGEIQQLRDDIKPLMNLALEAGD
                     AALSESAQPYVISGEKNLLDVEELSSNMKRLRELFDLFEQRSSLMRLLDVSSRAEGVK
                     IFIGGESGIATLDECSVITAPYTVDGQVVGSLGVIGPTRMAYERVIPIVDITARLLSS
                     ALSHKNEN"
     gene            3116..4219
                     /gene="hemH"
                     /locus_tag="PLN02_02340"
     CDS             3116..4219
                     /gene="hemH"
                     /locus_tag="PLN02_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOI51190.1"
                     /translation="MPRFQPEPPHRHGSPRGTALVLVNLGTPDAATAPALRRYLGQFL
                     ADPRVVEIPRLLWWPILNGIILNTRPKKSAAKYASVWTRDGSPLRVYTERQAKLLQGL
                     LGERGHRVSVAWAMRYGQPSVGSVLDRLKAAGHERILIVPMYPQYAASTTATVVDEAA
                     RWLLQTRNQPEMRFLRHYHDDTGYLAALAASVRKHWQQVGRPATDDRLLISFHGLPKR
                     SLELGDPYFCECHKTGRLLAEHLGLAADQYLITFQSRFGKAEWLQPYTAPTLEAWGKA
                     GVRRVDVICPGFSADCLETLEEIAQEGRDDFLKAGGKEYHYIPALNDAPDWIAALADI
                     TERHLAGWDTRGEPDAASLATEASEARRFGAPS"
     gene            4272..4715
                     /gene="trxC"
                     /locus_tag="PLN02_02345"
     CDS             4272..4715
                     /gene="trxC"
                     /locus_tag="PLN02_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOI51191.1"
                     /translation="MSDTLHLVCPHCAAVNRLPAARLSAAPNCGQCKQPLFTGEPLEV
                     DGASFERHLQRSELPLVVDFWAPWCGPCRMMAPTFHQAAIDLEPAARFLKVNTEEEQA
                     LAGRFNIRSIPTLAVFRNGREIARQSGALDLASLKRWLQPHLMAL"
     gene            4769..>5216
                     /locus_tag="PLN02_02350"
     CDS             4769..>5216
                     /locus_tag="PLN02_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOI51192.1"
                     /translation="MKIASSELQFTASHSSAQSREIEESLKMWSGPQRPDFENRGRGN
                     VGGERVVLSDAGKAAAAADSAAPVDETLDEDPKTLLIRLMLEMMTGKSVRTISASELS
                     GKSAPAAPQAPPTAAANNGRPATAGFGIEYDYRASYSESESTSFAAN"
BASE COUNT          922 a         1887 c         1616 g          791 t
ORIGIN      
        1 gcccatgtcg gcggcaacct gctgtcgccc ggccgacaga cgctgtgcca gcgtccggta
       61 ggcggcctgc gccgcatcgc tcgccgcgcg tagcgcgtcg acgtcggcag cggcggccag
      121 ttcggccagc cgcttctgcc aaccggcgag cagggccggc aattccggcg gcgaaacgcg
      181 gtggcgacgg acctgggcga gaatcgcctc gatccgcttc tcgacatccg ccaggcgccc
      241 gggatcgaga tcgacgcggt cggcgtagcg gcgcagcgtt gacaccgcat cggcgagttc
      301 ggcctgcatc gaggccagca cgccagcgac gtcggccagc gccgggtcgt aggcggccag
      361 gtcggcgagc cgcgtcgcca cctggtcgat ctgccgttcg caggcggcat cgtcgtcggc
      421 cagcacggcg agcgcgaact gcgcgccttc gatcaggctg gcggcatggg ccaggcggcg
      481 atgctcgcct tcgagcgtct cccactcgtc ggcggtaaac gccagcgccg ccagttcgcc
      541 aacctgccat tcgagctgtt cgcgttcgcg ctgcagcgcc tgtacgccat cattcgccgc
      601 ctgccaggcc tgttcggcat cgcgccaggt tttccaggcg gcagcgactt cgcgccccag
      661 gtcgagcagg ccgccgtgac tgtcgagcaa ctgacgctgg gcatcggccc gcaacaagga
      721 ctgatgggcg tgctggccgt ggatgtcgac cagccattcg ccgacttcgc gcaactgctg
      781 gacggtggcc ggcgagccgt tgatccaggc gcgcgaacgg ccgttggcgt cgaccacgcg
      841 gcgcagcaac aggtcttcgc tggcgtccag gccgttctcg tcgagccagg cagtcaccac
      901 aggcaggccg gcgatgtcga aagtggcggc gatctccgcc ttgtcgcaac cggcgcgtac
      961 gacgccggcg tcgccgcgct cgccgagcgc cagcgccagg gcgtcgatca ggatcgactt
     1021 gccagcaccg gtttcgccgg tcagcgcgcc aaaaccggcg gcgaactcca gctccagccg
     1081 gtcgacgatg acgaaatcgc gcagggtcag gtggcgcagc atcagcttcc cttaggcctt
     1141 tcgctccact gcagcttctg gcgcaacatc gcgaaatagc tgtagccggg cgggtgcaag
     1201 aagcagatgg tgtgctcgga acggtacagg cggacgctgt cgcccggttg caggtcgagg
     1261 gttagctggc catcgaagtg cacgcgcggc tcgtcggcgc ggacgacacg cagctcgatg
     1321 tcggcgttgt cgttgacgat caccggccgg ttggtcagcg catgcggaca cagcggcacc
     1381 atcgcaatgc cggtcagcgt cggatgcagg atcggcccgc cggcagacag cgaataggcg
     1441 gtcgagccgg tcggcgtgga cacgatcagg ccgtcggagc gcaggttgta gatgaactcg
     1501 ccgtcgatga acagctcgag ctcgatcatc cggccgatcg cccccttgtc gacgacaacg
     1561 tcgttgagcg ccacgttgct tgccagttcc ttgccgtcgc gcaccacttc ggcggccagc
     1621 agcatccggt tctcgcgctg gaatcggcca tcgagcaggt cgtccatgca actgagcatg
     1681 tcgtcgcggg cgatgtcggt catgaagccg agccgcccct ggttgacgcc gaccagcggc
     1741 acgcagtagc gggccagccg gcgcgccgcg ttgagcatgg tgccgtcgcc gccgaggacg
     1801 atggcggcgt cggcatgggc gccgatgtca ttgaaaccgc aggtgaccca gcgcgccaaa
     1861 tcgacccgct tgccgatatg ctcggcggtc tcgcgctcga cgaagaccga gacgccgcgc
     1921 tcgtgcagat actcggccag acggcgcagg gactcggcga tttccgggct gtggtatttg
     1981 ccgaccaggg cgatcgtgcg gggatgacgg aggcggttca tgggtgcgaa ttcttgcata
     2041 aaaaacgcct tgccgtgccg ccgccgcttt tcggtagaat cggcggcatg cttgatgatc
     2101 gctcgcgcac cctgctcaag actctggtcg aacactacat tgccgacggg caaccggtcg
     2161 gctcgcgcgc cctgtccaaa ttctccgggc tcgaactgtc gccggcgacg atccgcaacg
     2221 tcatggccga cctcgaagaa gccggtttcg tcgccagccc gcacacctcg gccgggcgca
     2281 tcccaaccgc ccgcggctac cggctgttcg tcgacaacct gctcaccgtg cagccgctcg
     2341 aagcgcacca gcgcggcgcc atcgaagatg cgctgcacgg gctgccgtcg aagcagatca
     2401 tcgccaacgc ctcgcaactg ctgtccagcc tgacccattt cgccggcgtc gtcatcgctc
     2461 cccggcgcac cgccaaccgc atccggcaga tcgaattcct cagcctgtcg gacaaacgca
     2521 tcctgctcat catcgtcacc gccgacggcg acgtgcagaa ccgcatcctc aattgcgagc
     2581 gcgcctactc gccgagcgag ctgaccagcg ccgccaacta cctgaaccag aatttcaccg
     2641 gcctcgactt cgagcagatc cgccagcgtc tggccggcga gatccagcaa ctgcgcgacg
     2701 acatcaagcc gctcatgaac ctggccctcg aggccggcga tgcggcgctc agcgaaagcg
     2761 cccagcccta cgtgatcagc ggcgagaaaa atctcctcga tgtcgaagag ctttcgtcca
     2821 acatgaaacg cctgcgcgaa ctgttcgacc tcttcgagca gcgctccagc ctgatgcgtc
     2881 tgctcgacgt ttcgagccgc gccgaaggcg tcaagatctt catcggcggc gaatccggca
     2941 tcgccaccct cgacgagtgc agcgtcatca ccgcgcctta cacggtcgac ggccaggtcg
     3001 tcggctcgct cggcgtcatc ggaccgaccc ggatggccta cgagcgcgtc atccccatcg
     3061 tcgacataac cgcccgcctg ctctcctcgg cactctccca caagaacgaa aactgatgcc
     3121 cagattccag cctgaaccgc cgcaccgcca cggcagcccg cgcggcaccg ccctcgtcct
     3181 ggttaacctc ggcacccccg acgccgcgac ggcgccggcg ctgcgccgct acctagggca
     3241 gttcctcgcc gacccgcgcg tcgtcgagat tccgcgcctc ctctggtggc cgatcctcaa
     3301 cggcatcatc ctcaacacgc ggccgaagaa atccgccgcc aagtacgcca gcgtctggac
     3361 ccgcgacggt tcgccgctgc gcgtgtacac cgaacgccag gccaagctgc tccaaggcct
     3421 gctcggcgaa cgcggccacc gcgtcagcgt cgcctgggcg atgcgctacg gccagccctc
     3481 cgtcggcagc gtgctcgacc gcctgaaggc cgccggccac gaacgcatcc tgatcgtccc
     3541 gatgtacccg caatacgccg ccagcaccac cgccaccgtc gtcgacgagg ccgcccgctg
     3601 gctgctgcag acccgcaacc agccggaaat gcgcttcctg cgccactacc acgacgacac
     3661 cggctacctg gcggcgctcg cagcctcggt gcgcaaacat tggcagcagg ttggccggcc
     3721 cgccaccgac gaccgcctgc tgatcagctt ccacggcctg cccaaacgct cactggagct
     3781 cggcgacccc tacttctgcg aatgccacaa gaccggccgc ctgctcgccg aacacctcgg
     3841 cctcgctgcc gatcaatacc tgatcacctt ccagtcgcgt ttcggcaagg ccgaatggct
     3901 acagccgtac acggcaccga ccctggaagc ctggggcaag gccggcgtgc ggcgggtcga
     3961 cgtgatctgc cccggtttct ccgccgactg cctggagacg ctggaagaaa tcgcccagga
     4021 aggccgcgac gacttcctca aggccggcgg caaggaatac cactacatcc cggcgctcaa
     4081 cgacgcgccg gactggatcg ccgccctcgc cgacattacc gaacgccacc tcgccggttg
     4141 ggacacccgg ggcgaaccgg atgccgccag cctcgccacg gaagccagcg aagcccgacg
     4201 cttcggcgcg ccctcttgaa atctggggcg gcgcctatat atcaggattc actgatacaa
     4261 cggagcccct catgagcgat accctgcatc tcgtctgccc gcattgcgcg gcggtcaatc
     4321 gcctgcctgc cgccaggcta agcgccgccc cgaactgcgg ccagtgcaag cagccgctgt
     4381 tcaccggcga gccactggaa gtcgacggtg ccagtttcga gcgccatctg cagcgcagcg
     4441 aactgccgct ggtcgtcgat ttctgggcgc cctggtgcgg cccctgccgg atgatggcgc
     4501 caaccttcca ccaggcggca atcgacctcg aaccggcggc ccgcttcctc aaggtgaaca
     4561 cagaggaaga gcaggcgctc gccggccgtt tcaacatccg cagcatcccg accctggccg
     4621 tcttccgcaa cggccgcgag atcgcccggc aaagcggcgc gctcgacctc gcctcgctca
     4681 aacgctggct gcaaccccac ttgatggcgc tttagttttc cccggcggcc gccgttaaca
     4741 atgtagtgaa caatgactgg aggccgccat gaaaatcgcc agcagcgaac tgcaattcac
     4801 cgcctcgcat agcagcgcgc aatcgcgcga aatcgaggag tcgctgaaga tgtggagcgg
     4861 cccgcaaaga ccggatttcg aaaaccgtgg ccgcggcaat gtcggcgggg aacgcgtcgt
     4921 cctctccgat gccggcaagg cggcagcggc agccgatagc gccgcaccgg tcgacgaaac
     4981 cctggacgaa gacccgaaga ccctgctgat tcgcctgatg ctggaaatga tgaccggcaa
     5041 gtcggtacgc accatcagcg ccagcgagtt gtccggcaaa tccgctccgg ctgcgccgca
     5101 agccccgccg acagccgcgg cgaacaacgg ccggccagcc actgccggct tcggcatcga
     5161 gtacgactac cgcgcatcct acagcgagag cgaaagcaca agcttcgccg ccaacg
//
LOCUS       DAOSDX010000068         5190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5678_length_5190_cov_3.014411, whole genome shotgun sequence.
ACCESSION   DAOSDX010000068 DAOSDX010000000
VERSION     DAOSDX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5678_length_5190_cov_3.014411"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1279
                     /gene="urtA"
                     /locus_tag="PLN02_02355"
     CDS             17..1279
                     /gene="urtA"
                     /locus_tag="PLN02_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HOI51193.1"
                     /translation="MNTRRNFIKATVLAAALSSIGMTAHAADTIKVGILHSLSGTMAI
                     SETALKETALMTIAEINAKGGVMGKKLEPVVVDPASNWPLFAEKARQLLTQDKVAVTF
                     GCWTSVSRKSVLPVYKELNGLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSK
                     DGGEAKRFVLLGTDYVYPRTTNKILRAFLKSKGVAEADIMEEYTPFGHSDYQTIIAKI
                     KKFASEGKKTAVVSTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPL
                     VGHLAAWNYFQSLKNPENTKFIKMYKDWAAKQKLPNASTVVTNDPMEATYIGIHMWAQ
                     AVEKAKSTDTDKVIAAMAGQSFKAPSGFTVKMDEKNHHLHKPVFIGEVKADGQFNVVW
                     KTKGPVRAAPWSPYIPGNDKKKDVPEGK"
     gene            complement(1333..2010)
                     /locus_tag="PLN02_02360"
     CDS             complement(1333..2010)
                     /locus_tag="PLN02_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HOI51194.1"
                     /translation="MSEYLPAIELEQGSKPQFAVIWLHGLGADGSDFVPVAPALGLAE
                     SPGVRFVFPHAPVMPVTCNGGWAMPAWYDILSLTPGERRIDEAGLLRSRAALRRLIER
                     EGERGIPAARVFLAGFSQGGAVAYTTALTHPQALGGVIALSTYLPSPGLVEREATEAN
                     RGIPVFAAHGDDDDVVAPALGRQARDFLQAAGYAVGWREYPMAHEVCFEEIAAIGDWL
                     RERFAAA"
     gene            complement(2088..2648)
                     /locus_tag="PLN02_02365"
     CDS             complement(2088..2648)
                     /locus_tag="PLN02_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOI51195.1"
                     /translation="MTQAIPDSMTLGQLCRQVGLARSSVLHYEALQLLAPSGRTAAGY
                     RRYGAAELARLRTIRRLRDAGLTLADIHTLLVPPGDSAASRPVALLEKRLLELCQEAA
                     RLHAQQRILARLLATPEFRDRRQSYDKAAWVALLRRAGFDEAAMDLWHGEFERENPAG
                     HAAFLKSLGLEEGAIDAIRQRAQARG"
     gene            complement(2645..3061)
                     /locus_tag="PLN02_02370"
     CDS             complement(2645..3061)
                     /locus_tag="PLN02_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51196.1"
                     /translation="MSDPKQMSNAQFLAAFLDASMPAAGFDHYGHLRAAWLLLQREPL
                     ERAVSDTCEAIARLAARLGVPGKYHRTLSEALVRLMAEGGGADPVLSWDDFLVANGEL
                     VNDARAVLARYYSASLLNSPAARERFLPPDRQPLPS"
     gene            3262..3957
                     /locus_tag="PLN02_02375"
     CDS             3262..3957
                     /locus_tag="PLN02_02375"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HOI51197.1"
                     /translation="MDHPDQLRWKQNWRANRIDFHLDHVHPLLLRCWAEIGVAPAARV
                     FVPLCGKSLDLMWLHGQGHDIVGVELSTVAVNAFFRESRLRPQRSAHGALECWAQERL
                     TIHCGDFFALRRQDLVGVSAVYDRAALTALPEALRPAYVAHLRAILPDDCRILLLTVE
                     DLDDGESEADASGSSAEVEGLYAAHFRVRLLHVEHCAALLAGNGTISEPRSVHKAYLL
                     GGDESAPPVLFSD"
     gene            complement(3947..4123)
                     /locus_tag="PLN02_02380"
     CDS             complement(3947..4123)
                     /locus_tag="PLN02_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51198.1"
                     /translation="MNIENRRHEALHLLAAARITALPYGNAWWLVGDGISHVVGELAG
                     ISPDHLTRFAVVNR"
     gene            complement(4218..4421)
                     /locus_tag="PLN02_02385"
     CDS             complement(4218..4421)
                     /locus_tag="PLN02_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOI51199.1"
                     /translation="MANGTVKWFNDSKGFGFITPDEGGEDLFAHFSAIQGQGFRTLAE
                     GQRVSFDVTTGQKGKQAGNIRAA"
     gene            4791..>5190
                     /locus_tag="PLN02_02390"
     CDS             4791..>5190
                     /locus_tag="PLN02_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51200.1"
                     /translation="MNREEFFSSIDAKISRASRADTGSADMPAENQAFLETVVARLQP
                     VVASYQAKLAERQISAEVKSFPACISFTLRYRDGGHCGLTLDGRLADRRIEITAHYTN
                     DNGRNYVNTGGVTYGKDNWQDSIFEEALQKC"
BASE COUNT         1032 a         1733 c         1616 g          809 t
ORIGIN      
        1 agatccagga gcactcatga acactcgtcg caacttcatc aaggccacag tcctggccgc
       61 cgccctctcc tccatcggca tgacggcgca cgccgccgac acgatcaagg tcggcatcct
      121 gcactcgctg tccggcacca tggcgatttc cgaaacggcg ctcaaggaaa ctgcgctgat
      181 gaccatcgcc gaaatcaacg ccaagggcgg cgtcatgggc aagaagctgg aaccggtcgt
      241 cgtcgacccc gcctccaact ggccgctgtt cgccgaaaag gcccgccagc tgctgaccca
      301 ggacaaggtc gccgtcacct tcggctgctg gacctcggtg tcgcgcaagt cggtcctgcc
      361 ggtctacaag gaactgaacg gcctgctctt ctacccggtc cagtacgaag gtgaagaact
      421 cgagaagaac gtcttctaca ccggcgccgc gcccaaccag caagccatcc cggccgtcga
      481 atacctgatg tccaaggacg gcggcgaagc caagcgcttc gtgctgctcg gcaccgacta
      541 cgtctatccg cgcaccacca acaagatcct gcgcgccttc ctgaagtcga agggcgtcgc
      601 cgaagccgac atcatggaag agtacacccc gttcggtcat agcgactacc agaccatcat
      661 cgccaagatc aagaagttcg cctccgaagg caagaagacc gccgtcgtgt cgaccatcaa
      721 cggcgactcc aatgtgccct tctacaagga actcggtaac gccggcctca aggcgaccga
      781 cgtgccggtc gtcgccttct cggtcggcga ggaagaactg cgcggcgtcg ataccaagcc
      841 gctggtcggc cacctggccg cctggaacta cttccagtcg ctgaagaacc cggaaaacac
      901 caagttcatc aagatgtaca aggactgggc cgccaagcag aagctgccga acgcatccac
      961 cgtcgtcacc aacgacccga tggaagctac ctacatcggc atccacatgt gggcgcaggc
     1021 cgttgaaaaa gccaagtcca ccgacaccga caaggtcatc gccgccatgg ccggccagtc
     1081 gttcaaggcg ccgtcgggct tcaccgtcaa gatggacgaa aagaaccacc acctgcacaa
     1141 gccggtgttc atcggcgaag tgaaggccga cggccagttc aacgtggtct ggaagaccaa
     1201 gggcccggtc cgcgccgccc cgtggtcgcc ctacatcccg ggcaacgaca agaagaagga
     1261 cgtgccggaa ggcaagtaag tcagcttttc ctggacgaag aacgaacggg gccgcggccc
     1321 cgtttttcgc attcaggcgg cggcgaaacg ctcgcgcagc cagtcgccga tggcggcgat
     1381 ttcctcgaag cagacctcgt gcgccatcgg atattcccgc caaccgaccg cgtaaccggc
     1441 ggcctgcagg aaatcccgcg cctggcgccc aagcgccggc gccaccacgt catcgtcgtc
     1501 gccgtgcgcg gcgaatactg gaatgccacg attggcttct gtcgcctcgc gctcgaccag
     1561 ccccggcgat ggcaggtaag tggacagcgc gatcaccccg cccagcgcct gtggatgcgt
     1621 cagcgccgtc gtgtaggcaa ccgccccacc ctgcgagaaa ccggccagga aaacccgcgc
     1681 tgccgggatg ccacgctcgc cctcgcgctc gatcaggcgg cgcagcgccg cccgcgaacg
     1741 caacaggccg gcctcgtcga tgcgccgctc gcccggcgtc agcgacagga tgtcgtacca
     1801 cgccggcatc gcccagccgc cattgcaggt caccggcatc accggcgcgt gcggaaagac
     1861 gaaacgcact ccgggcgatt ccgccaggcc gagcgccggc gccaccggca cgaagtcgga
     1921 accatcggcc cccaggccat gcagccagat gaccgcgaac tgcggcttgc tgccctgttc
     1981 gagttcgatt gccggcaagt attcgctcat ttcacaactc ctctgtcctg cccggcgttt
     2041 tacccggaaa cggcgggctg cgccagcgct tccggcgccc cgggatgtca cccccgggcc
     2101 tgcgcccgct gccggatggc gtcgattgcg ccttcctcca gcccgagcga cttgaggaag
     2161 gcggcatggc cggcggggtt ttcccgctcg aactcgccgt gccacagatc catcgccgcc
     2221 tcatcgaagc cggcgcggcg cagcaaagct acccaggcgg ccttgtcgta cgactgacgc
     2281 cggtcgcgga actcgggcgt ggccagcagg cgggccagaa tccgctgctg ggcgtgcagc
     2341 cgcgcggctt cctggcagag ctcgagcaag cgcttttcca gcagcgccac cggccgggaa
     2401 gctgcgctgt cgccgggcgg gaccaagagg gtgtggatgt cggcaagcgt caggccggcg
     2461 tcgcgcaggc gccggatcgt gcgcaggcgc gccaactcgg cggcgccata gcgccgatag
     2521 ccggcagcgg tacgcccgct cggcgccaac aactggagag cttcgtagtg gaggacggaa
     2581 ctgcgcgcca ggccgacttg ccggcaaagc tgtccgagcg tcatcgagtc gggaatggcc
     2641 tgggtcatga tggcagcggc tggcggtcgg gcggaaggaa gcgttcccgt gccgccggcg
     2701 aattgagcag cgatgcggaa taatagcgcg ccaggacggc gcgggcgtcg ttgaccagct
     2761 ctccgttggc gaccaggaag tcatcccagg aaagcaccgg atcggcgccg ccaccttcgg
     2821 ccatcaagcg gaccagggct tcggagaggg tgcgatggta tttcccgggc acgcccaggc
     2881 gcgccgccag ccgggcaatg gcctcgcagg tatcgctgac cgcccgttcc agcggctccc
     2941 gttgcagcag caaccaggct gctcgcagat gaccgtagtg gtcgaagccg gcagcaggca
     3001 tgctggcgtc gagaaaggcg gcaaggaact gggcgttcga catctgtttc ggatcggaca
     3061 tgatgagact ccggtgaaat ggaaagcgcc agtctcaacc ctgcgactgt cgtcaggtca
     3121 acaccacttc cgtttgccgc cagccccgca tgcgcgcata gtggaggggc ttgaatctgc
     3181 gcccggcgcc ttgaaaagct tccgcgaccg cgcggcgatg ccgccgccgt cgtcctccga
     3241 cgctttccga aaggccccgc catggaccat cccgaccagt tgcgctggaa acagaactgg
     3301 cgcgcgaacc gcatcgattt tcaccttgac catgtccatc ccctgctctt gcgctgctgg
     3361 gccgaaatcg gcgtcgcgcc ggcagcgcgc gtcttcgtgc cgctttgcgg caagagcctc
     3421 gacctgatgt ggttgcacgg ccaggggcat gacatcgttg gcgtcgaact gagtacggtg
     3481 gcggtcaacg cctttttccg ggaaagccgc ttgcgcccgc aacgcagcgc gcacggcgcg
     3541 ctggaatgct gggcgcagga acggctgacc atccactgcg gcgatttctt cgcgctgcgt
     3601 cggcaagacc tggtcggcgt cagcgcggtt tacgatcgcg ccgcgctgac cgcgctaccg
     3661 gaagccctgc gccccgctta cgtcgcccac ctgcgggcga tcctgcccga cgactgccgc
     3721 attctgctgc tgacggtcga agatctcgat gacggggaaa gcgaagccga tgccagcggc
     3781 agttcggcgg aagtggaagg tctgtacgcg gcgcatttcc gcgtcagact gctgcatgtc
     3841 gagcattgtg ccgcgctgct ggccggaaac ggcacgatca gcgagccgcg ctcggtgcat
     3901 aaggcgtatt tgctgggcgg ggacgaaagc gcgccgccag tgctgttcag cgattgacga
     3961 cggcgaagcg ggtcagatga tcgggggaaa tgccggcgag ttcgccgacc acgtggctga
     4021 tgccgtcgcc gaccagccac caggcgttgc cgtagggtaa tgcggtaata cgcgcagcag
     4081 ccagcagatg caaggcttcg tgccggcgat tttcaatatt catgggtgtg gccacaaggc
     4141 ctcctgtctc aataaaacaa aaccccggcg atgccggggt tgcagtgcca gcgggccgac
     4201 cagcggcccc gccggtatca ggcagcgcgg atgttgccgg cctgcttgcc cttctgaccg
     4261 gtcgtcacgt cgaagctgac gcgctgacct tcggcgaggg tgcggaaacc ctggccctga
     4321 atggcggaaa aatgagcgaa aaggtcttcg ccgccctcat cgggggtgat gaagccgaaa
     4381 cccttggaat cgttgaacca cttgacggtg ccgttggcca tgtctgttat ctccggtaaa
     4441 aaatgaatcg gggcccgagc ccctgtaaat ggggcgagat caagacggcg aggtcagaat
     4501 ggggagaaac gccgcaacaa cggggctaaa ccggcagaaa acgacactgt ttgaaaatcg
     4561 ctgcggtgca caatacgcgt tgccaagaga aatagcaaag caattctcga cagcccggtc
     4621 attttccgcg cgggaaagtc cccaaagcgt ctgccgacga tggcataatc gcccggattc
     4681 caaaaacgac aagacttccc ctccccaatc agacagcagt tcgactgcgc gggcacgccc
     4741 gcgcggcggt cgccagacca aggaatttca acacgtcagg gaggtgtgca gtgaatcgcg
     4801 aagagttctt cagcagcatc gatgccaaga tttcccgtgc cagccgagcc gataccggtt
     4861 ccgccgacat gccggccgaa aaccaggctt tcctggaaac ggtggtcgcc cgcctgcagc
     4921 cggtggtcgc ttcctaccag gccaagctcg cggaacggca gatcagcgcc gaggtcaaga
     4981 gtttccccgc ctgcatttcc ttcaccctgc gctatcgcga cggtggccat tgcggcctga
     5041 ccctcgacgg ccgcctggcc gaccggcgca tcgagattac cgcccactac accaacgaca
     5101 acggccgcaa ctacgtcaat accggcggcg tcacctatgg caaggacaac tggcaggaca
     5161 gcatcttcga agaagcgctg cagaaatgca
//
LOCUS       DAOSDX010000069         5187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5682_length_5187_cov_3.826578, whole genome shotgun sequence.
ACCESSION   DAOSDX010000069 DAOSDX010000000
VERSION     DAOSDX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5682_length_5187_cov_3.826578"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /gene="ssrA"
                     /locus_tag="PLN02_02395"
     tmRNA           <1..223
                     /gene="ssrA"
                     /locus_tag="PLN02_02395"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01850"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="betaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01850"
     gene            complement(241..816)
                     /locus_tag="PLN02_02400"
     CDS             complement(241..816)
                     /locus_tag="PLN02_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI51201.1"
                     /translation="MGLFRIDRKTPATAGPRRLDCGVSLYRAGDEGVAWRLRRGVLRL
                     DSPQAGGERFASLAIANDIVGCETLLFGVYTFSASALTDCEIVPWPEGEAGLAGDSLL
                     ATLARAQQRAAEVVALRGGQAVDRVLALVRLLVESDGQVVLPTRQAIADITDLRFETV
                     SRVIKQLERSGILNPARVVGVHATRSFHYAG"
     gene            complement(883..3186)
                     /locus_tag="PLN02_02405"
     CDS             complement(883..3186)
                     /locus_tag="PLN02_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51202.1"
                     /translation="MSFSVRPLAAAIGFAFSSLALSLPATAQTLGEITVSAERDKSAG
                     STTQLDAAELRRRGVRNSDVASLLQEVPGVSFYGAGAVSSLPVIHGLADDRLRIKVDG
                     MDFIAACPNHMNPALSYLDPSQVGALTVFAGISPVSVGGDSIGGSIVAETRAPEFAAP
                     GERLVKGEVGAFYRSNNDARGVNASATVASDSLSLSYSGAYSAADNYSAGGNFKNYTA
                     TGRAGHRLDRDEVGSTSYRSGNHTLGLAFRNANHLFEAKLGYQDMPEQLYPNQRMDLL
                     DNEQKRINLRWLGDYDWGKLEARVYHETVDHFMDFGDDKRYWYGSNSGAIDGSPCTPI
                     RYTGDPAGTCAAGMPMKTASKTTGASLKADVQLGSKDLLRVGGELQKYRLDDWWPASG
                     GGMGPGTFVNINDGQRDRSALFAEWESRRSERWTTSLGLRFERVAMDAGKVRGYDVDP
                     APPGSFGMTSADAARFNASDRSRHDNNWDFTALARYAASNSLDIDIGFARKTRSPGLY
                     EAYPWSTWPMAATMNNYVGDGNGYVGNPDLDPEKAYTLSTTFDWHNAERSRELKLTPY
                     YTHVSDYIDAVQWNAATNAPRTTLLTDQFTVLRYVNQSARLYGLDLYGKTPLASTGFG
                     QFGLKGVLSYTRGKNRKTGDDLYNIMPLNATLTLTQKLAGWENALEVVGVAAKNKVSD
                     MRNEVKTAGYGLVNLRLSHTWKQVRLDFGVENLFDRYYEMPLGGAYLGQGRTMSMNPP
                     VADGMIGWGNPVPGMGRSLYAGINIKF"
     gene            complement(3303..5045)
                     /gene="ptsP"
                     /locus_tag="PLN02_02410"
     CDS             complement(3303..5045)
                     /gene="ptsP"
                     /locus_tag="PLN02_02410"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOI51203.1"
                     /translation="MSFILHGLGVSTGIAIGRAMLMSHATLEVSHLTLAPRLVDKEIA
                     RFDAAVAAVKQELVQMREHAEHAPAELGAFIDIHTMFLEDNELVGKPREIIRERRCNA
                     EWALVQQMEHLVGQFEEFDDPYLRERRFDVIQVVERVIKELLGHPGRASLKAAKQTKE
                     ESLIVVAHDLSPADTIAFKDHRFASFITDVGGATSHTAILARSMAIPAVLGLENARAL
                     IRDGEQLIVDGLRGVVIVNPDPRVLDEYRLRAEQIELEKTKLRQLRSAPTQTIDGIEV
                     ALHANIELPGDVPDALDGGAEGIGLFRTEFLFLDRGDMPDEQEQYEAYKKVVKGMAGR
                     PVTIRTFDLGADKDIKPEATAGDRVKTNPALGRRAIRLSLAEPKMFQTQLRAILRASR
                     FGPIKLLIPMLAHAHEIDQTLAAVEQAKSSLRGEKIAFDEHIQVGGMIEIPAAALAVG
                     IFLRRLDFLSIGTNDLIQYTLAIDRSDEQVAALYDPLHPAVLMLLAHTISSAEKAGVP
                     VSVCGEMAGDPKLTRLLLGMGLRIFSMHPSQILKVKSRVLKSELAELAPNVRKMLRLD
                     EPARLREALEKLNA"
     gene            complement(5051..>5187)
                     /locus_tag="PLN02_02415"
     CDS             complement(5051..>5187)
                     /locus_tag="PLN02_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOI51204.1"
                     /translation="SIMGVMMLAAGKGSTVTIETDGKDEAEAMEALLALIADYFGEGE
                     "
BASE COUNT          875 a         1623 c         1753 g          936 t
ORIGIN      
        1 tttgcggggc aaagaatgat tatcaaaaaa atttgggggt gtactggttt cgacagggcg
       61 gacaaaggtg cgcaggcaac tcgaaaggcg acggacgtaa tccgagcaaa tccataattg
      121 ccaacgatga gcaattcgct attgccgcct aaaaacggat agccggggct gctgaagcct
      181 tgttaccaaa gacagccggc ggggacttcg gtccccgtcg tcacgtttac gaccggcaat
      241 tcagccggcg taatggaagc tgcgcgtggc atggacaccg acgacgcgcg ccggattgag
      301 gatgccgcta cgctccagtt gcttgatcac ccgcgacacc gtctcgaagc gcaggtcggt
      361 gatgtcggcg attgcctgcc gcgtcggcag caccacctgc ccgtcgcttt cgaccagcag
      421 acggaccagc gccaggacac gatcgaccgc ctggccgccg cgcaacgcca ccacctcggc
      481 cgcccgctgc tgggcgcggg ccagcgtggc gagcagggaa tcgccggcca gcccggcctc
      541 gccctccggc caggggacga tttcgcagtc ggtcagggcg ctggccgaga aggtatagac
      601 gccgaacagc agggtttcgc agccgacgat gtcgttggcg atggccaggc tggcgaagcg
      661 ctcgccgccc gcctgcgggc tgtcgagacg gagcacgccg cggcgcaggc gccaggccac
      721 gccttcatca ccggcgcggt agaggctgac gccgcagtcg agacgacgtg gtccggcagt
      781 cgccggggtc ttgcgatcga tcctgaacaa gcccatcttc cactcctcca caaacgacaa
      841 aaccccggtc gggatcgacc ggggtcattt caggcaattg cctcagaact tgatgttgat
      901 gccggcatac agcgagcggc ccatgccggg caccgggttg ccccagccga tcatcccgtc
      961 ggccaccggc ggattcatcg acatcgtccg cccctgaccg aggtaagcgc cgccgagcgg
     1021 catctcgtag tagcgatcga ataggttttc aaccccgaag tcgagacgga cttgcttcca
     1081 ggtgtggctc aaccgcaaat tgaccagccc gtaaccggcg gtcttgacct cgttgcgcat
     1141 gtcggacacc ttgttcttcg ccgccacgcc gacgacctcc agcgcattct cccagccagc
     1201 cagcttctgg gtcagcgtca gcgtcgcgtt gagcggcatg atgttgtaga gatcgtcgcc
     1261 ggtcttgcgg ttcttgccgc gcgtgtagct caacacgccc ttcaggccga actggccgaa
     1321 accggtgctg gccagcggcg ttttgccgta caggtcgaga ccgtagaggc gggcggactg
     1381 attgacgtag cgcagcactg tgaactggtc ggtgaggagc gtggtgcgcg gcgcgttggt
     1441 ggcggcgttc cactggacgg cgtcgatgta gtcgctgaca tgcgtgtagt aaggcgtcag
     1501 cttgagttcg cggctgcgct cggcgttgtg ccagtcaaag gtggtcgaca gggtgtaggc
     1561 cttttccgga tcgagatccg gattgccgac gtaaccgttg ccatcgccga catagttgtt
     1621 catcgtcgcc gccatcggcc aggtcgacca cggataggcc tcgtacaggc cgggcgaacg
     1681 cgtcttgcgc gcgaaaccga tgtcaatatc gagcgagttg ctggcggcgt agcgggccag
     1741 ggcggtgaaa tcccagttgt tgtcgtggcg cgaacggtcg ctggcgttga aacgcgcggc
     1801 atcggcactg gtcatgccga aacttcccgg cggcgccggg tcgacgtcgt agccgcgcac
     1861 cttgccggca tccatcgcca cccgttcgaa acgcaagccc agcgacgtgg tccagcgttc
     1921 gctgcgacgg ctttcccatt cggcgaacaa ggcgctgcgg tcgcgctggc cgtcgttgat
     1981 gttgacgaag gtcccgggcc ccataccgcc gccggaagcc ggccaccagt cgtcgagacg
     2041 gtatttctgc agctcgccgc cgacgcgcag cagatccttg ctgccgagtt gcacatcggc
     2101 cttcaggctg gcgccggtgg ttttgctggc ggtcttcatc ggcatcccgg cggcgcaggt
     2161 accggccggg tcgccggtat agcggatcgg cgtgcaaggc gaaccatcga tagcgccgga
     2221 attcgagccg taccagtaac gtttgtcgtc gccgaagtcc atgaagtgat cgacggtctc
     2281 gtgatagacg cgggcttcca gcttgcccca gtcgtagtcg ccgagccagc gcaggttgat
     2341 gcgcttctgc tcgttgtcga gcaggtccat gcgctggttc gggtagagct gctccggcat
     2401 gtcctggtag ccgagcttgg cttcgaacag gtggttggcg ttgcggaagg ccaggccgag
     2461 agtgtggttg ccgctccggt aactggtcga accgacctcg tcgcgatcaa ggcggtggcc
     2521 ggcgcgaccg gtggcggtgt agttcttgaa gttgccgccg gcgctgtaat tgtcagcggc
     2581 actgtaagcg ccgctatagc tcaggctgag gctgtcgctg gcgacggtgg cgctggcgtt
     2641 taccccgcgg gcgtcgttgt tgctgcgata gaaggcgcca acttcgccct tgaccaggcg
     2701 ctcgccgggc gcggcgaatt ccggggcgcg cgtctcggcg acgatgctgc cgccgatgct
     2761 gtcgccaccg acgctgaccg ggctgatccc ggcaaagacg gtgagggcgc cgacctgact
     2821 cggatcgagg taggagagcg ccgggttcat gtgattgggg caggcggcga tgaaatccat
     2881 gccgtcgacc ttgatgcgca ggcggtcgtc ggccaggccg tggatcaccg gcaggctgga
     2941 aacggcgccg gcgccgtaga agctgacgcc gggaacctcc tgcagcagcg atgcgacatc
     3001 gctgttgcgt acgccacgcc ggcgcagttc ggcggcgtcg agctgggtgg tgctgccggc
     3061 gctcttgtcg cgctcggcgc tgacagtgat ttcaccgagg gtctgcgccg ttgcgggaag
     3121 actcagggcg agcgaggaaa aggcgaagcc gatggccgcc gccagcggcc gaacggaaaa
     3181 agacatacga acccccaatt atttttgatc gacgacaagg gggcgattgt cggcaaggaa
     3241 gctgcccacg ggaatgtgac aaatcgtcgc acccctgcgg cagcgggcgt cacgccgctg
     3301 gttcaggcgt tgagcttttc cagcgcctcg cgcaaacggg ccggctcgtc caggcgcagc
     3361 atcttgcgca cattgggcgc cagttcggcg agttccgact tgaggacgcg actcttgacc
     3421 ttgaggatct gcgacggatg catcgagaaa atgcgcaggc ccatgccgag cagcagccgg
     3481 gtcagcttgg ggtcgccggc catttctccg cacaccgaga ccggcacgcc ggccttttcg
     3541 gcgctggaaa tggtatgcgc cagcagcatc agcaccgccg ggtgcagcgg gtcgtagagc
     3601 gcggcgacct gctcgtcgga gcggtcgatc gccagcgtgt actggatcag gtcgttggtg
     3661 ccgatcgaca ggaagtcgag ccggcgcagg aagatgccga cggccagcgc ggcggcggga
     3721 atctcgatca tgccgccgac ctgaatgtgc tcgtcgaagg cgatcttctc gccgcgcagg
     3781 ctggatttgg cctgctcgac ggcggccagc gtctggtcga tctcgtgcgc gtgcgccagc
     3841 atcgggatca gcagtttgat cgggccgaaa cgcgaagcgc gcaggatcgc ccgcaactgg
     3901 gtctggaaca tcttcggttc ggccagcgac aggcggatcg cccgccggcc gagtgccggg
     3961 ttggtcttga cgcggtcgcc ggcggtggct tccggcttga tgtccttgtc ggcaccgagg
     4021 tcgaaggtgc ggatggtcac cggccgaccg gccatgccct tgaccacctt cttgtacgcc
     4081 tcgtactgct cctgctcatc gggcatgtcg ccgcgatcga ggaagaggaa ctcggtgcgg
     4141 aacaggccga tgccttcggc accgccgtcg agcgcgtcgg gcacatcgcc gggcagttcg
     4201 atgttggcgt gcagcgccac ttcgatgccg tcgatggtct gcgtcggcgc gctgcgcaac
     4261 tggcgcagct tggtcttctc gagttcgatc tgctcggcac gcaagcggta ctcatcgagc
     4321 acgcgcggat cggggttgac gatgacgacg ccgcgcaggc cgtcgacgat cagttgctcg
     4381 ccatcacgga tcagcgcgcg ggcgttttcc aggcccagta cggcgggaat cgccatgctg
     4441 cgggcgagga tcgccgtgtg cgaggtggcg ccgccgacgt cggtgatgaa ggaggcaaag
     4501 cggtggtcct tgaaggcgat ggtgtcggcc ggcgacagat cgtgagcgac gacgatcagc
     4561 gactcttcct tggtctgctt ggccgccttc agtgaagcgc gcccgggatg gccgagcaac
     4621 tccttgatca cccgttcgac cacctggatg acgtcgaaac ggcgttcgcg caggtagggg
     4681 tcgtcgaact cctcgaactg cccgaccaga tgttccatct gctggaccag cgcccactcg
     4741 gcattgcagc ggcgctcgcg gatgatctcg cgcggtttgc cgaccagctc gttgtcctcg
     4801 aggaacatcg tgtggatgtc gatgaaggca cccagttcgg ccggagcgtg ttcggcgtgc
     4861 tcgcgcatct gcaccagttc ctgcttgacc gccgcgacgg cagcgtcgaa acgggcgatt
     4921 tccttgtcca ccagacgcgg cgccagcgtc aggtgcgaca cctccagcgt cgcatgcgac
     4981 atcagcatcg cacggccgat ggcgatgccg gtggaaacgc ccagtccgtg caggatgaag
     5041 ctcatcgcgc ctattctccc tcaccgaaat agtcggcgat caacgccagc agcgcctcca
     5101 tcgcctcggc ctcgtccttg ccgtcggttt cgatggtcac cgtcgagccc ttgccggcgg
     5161 ccagcatcat cacgcccatg atgctct
//
LOCUS       DAOSDX010000070         5141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5748_length_5141_cov_3.276249, whole genome shotgun sequence.
ACCESSION   DAOSDX010000070 DAOSDX010000000
VERSION     DAOSDX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5748_length_5141_cov_3.276249"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1136
                     /locus_tag="PLN02_02420"
     CDS             315..1136
                     /locus_tag="PLN02_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI51205.1"
                     /translation="MSENILVNIEDLHFTYDGRPVLQGINMRIPRGKVVAIMGGSGCG
                     KTTLLRCIGGQLRPSGGTVRLADAVVSKMSEAELYRLRRRMGMLFQFGALFTDLSVFE
                     NVAFPLREHTDLSDEMIRDLVLMKLEAVGLRGAHALMPGSLSGGMARRVALARAIALD
                     PMLVMYDEPFTGLDPIALGVIGRLIRTLNDALGATSIMVTHDVQESLQIVDYIYFLNG
                     GRVVAEGTPDEIRASRDPFVHQFVHAEPDGPVHFDYPAASMQADFSLGGGYAGAA"
     gene            1120..1914
                     /gene="mlaE"
                     /locus_tag="PLN02_02425"
     CDS             1120..1914
                     /gene="mlaE"
                     /locus_tag="PLN02_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HOI51206.1"
                     /translation="MPGPLDLLRKIGAPTVDRIWRLGFAARFFFAILTHSGTAFRRLP
                     LTLREIYFSGVLSLLIILVSGLFVGLVLGLQGYETLQRYGSTEALGILVALSLVRELG
                     PVVAGLLFASRAGSSITAEIGLMKTTEQLKAMDMMAVNPLARVVAPRFWGGVVSMPLL
                     AAMFSAMGVFGGWLIGVVFIGVDDGAFWSQMQSSVDFRYDILNGIIKSVVFGFAVSLI
                     AVFEGYDAVPTAEGVSRAITRTVVTSAMTILALDFILTSFMFRGTS"
     gene            1911..2381
                     /gene="mlaD"
                     /locus_tag="PLN02_02430"
     CDS             1911..2381
                     /gene="mlaD"
                     /locus_tag="PLN02_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOI51207.1"
                     /translation="MNRTVIDLWVGFFVALGIAAVLFLALKVGNLSAAHLSETYVLQA
                     KFDNIGGLKVRGPVRSAGVLVGRIADIRFDPESYQAVVTMNIDGRYRFPRDTFASIYT
                     AGLLGEQYIGFDVGGDEKMLQPGDTVKMTQSAVVLEKLISQFLFGKAAEGQDSK"
     gene            2378..3100
                     /locus_tag="PLN02_02435"
     CDS             2378..3100
                     /locus_tag="PLN02_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOI51208.1"
                     /translation="MTLKAGFSVRRLLRLLALGGALAVSTAHASAQEAQRDPYENFNR
                     SMFSVNEALDKYAARPVAQAYDAAFPLPVKASVGNFFGNVGEVWGGANNALQGKPADA
                     GLGLTRFLINSTVGILGLFDVASELGFERYNEDFGQTMAVWGVGNGPYVFLPVVGPRT
                     SRDLLGWAVDTYVDPVSTIHPESVRIGTSVLRFVDVRAGLLPADKVVEEAALDKYAYV
                     RDAYLQRRRNLIYDGRPPRLDD"
     gene            3116..3745
                     /locus_tag="PLN02_02440"
     CDS             3116..3745
                     /locus_tag="PLN02_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI51209.1"
                     /translation="MIKRLLAVCLACFLAAPLFAAEAPDALIRRVSEEVLEIVRQDKD
                     IRNGDIQKISALVDAKVLPYFNFQRMTSLAVGRDWRSANPAQKNRLAEEFKNLLVRTY
                     SNALSSYSNQRIVFKPFKMKSGETEVLVRSEVTQPGSQPVQIDYWLEQSGDNWKVFDV
                     VVAGISLVTNYREQFANEVRAGGIDGLIASLAAKNKSLEVAAAAKNGKK"
     gene            3742..4020
                     /locus_tag="PLN02_02445"
     CDS             3742..4020
                     /locus_tag="PLN02_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI51210.1"
                     /translation="MIERSNGALAVRGAMVMANARALLAAGCAEMGGDSVVDLSAVDA
                     ADSSALAVMLGWLRHAQAAKQRLSFVGVPAGVLSLAELYGISDLLPLA"
     gene            4026..4697
                     /locus_tag="PLN02_02450"
                     /pseudo
     CDS             4026..4697
                     /locus_tag="PLN02_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362073.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4748..>5141
                     /locus_tag="PLN02_02455"
     CDS             4748..>5141
                     /locus_tag="PLN02_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI51211.1"
                     /translation="MSINELLIGFWTLFYKEVLRFWKVAFQTVAAPVLTAMLYLMIFG
                     HVLEDHVRVHGIGYTSFLIPGLVMMQVLQNAFANSSSSLIQAKITGSIVFVLLPPIPY
                     GAFFAAYVLAALLRGLMVGVGVLLATVVF"
BASE COUNT          842 a         1507 c         1650 g         1142 t
ORIGIN      
        1 ggggacctgt cggtggcgga ctcatctgtt cgatgatcgg gggaatgtct ccgctgtcgg
       61 ggctggggcg cgcatgcaga tgcccggcca acgcttgccg gggaacgggc gcactgcacg
      121 cggagagcag cggaaccatg gcgaacagga gcgacatgaa tttgagcatg gcagtccgga
      181 cggggtgatt gcgtgatgct agatgcaaat tatctatttg tcatcttgtt tttggtcgcc
      241 ttgtctttgt ttcaaggaaa tgacgatagc gagttctgtt agaatgccgc caatcttttg
      301 cggggaagca ggcgttgtcg gaaaacatcc tggtcaatat cgaagaccta catttcacct
      361 atgatgggcg gccggtgctg caggggatca acatgcgcat cccgcgcggc aaggtggtgg
      421 ccatcatggg cggctccggc tgcggcaaga ccaccctcct gcgctgtatc ggcggccaat
      481 tgcggcccag tggcggaacg gttcggctgg ccgatgccgt ggtcagcaag atgtcggagg
      541 ccgagcttta tcgactgcgt cgacgcatgg ggatgctgtt ccagttcggc gccttgttca
      601 ccgatctgtc ggtgttcgag aacgtggcct ttccgttgcg cgagcatacc gatctttccg
      661 acgaaatgat ccgcgacctg gtgctgatga agctggaagc cgttggcctg cgtggcgccc
      721 atgccttgat gccgggttcg ctgtccggcg gcatggcgcg gcgggtggca ctggcccggg
      781 cgatcgccct ggatccgatg ctggtcatgt acgacgaacc cttcaccggc ctcgatccga
      841 tcgcgctcgg cgtcatcggc cgcctgatcc ggaccctgaa cgacgcgctg ggggcgacct
      901 cgatcatggt cacgcacgat gttcaggagt cgctgcagat cgtcgattac atctatttcc
      961 tgaacggcgg ccgggtggtt gccgaaggga cgccggacga aatccgtgcc tcgcgcgacc
     1021 cgttcgtgca ccagttcgtg catgccgagc ctgacgggcc ggtgcatttc gactatccgg
     1081 cagcgtcgat gcaggccgat ttttccctgg ggggcggata tgccggggcc gcttgacctg
     1141 ctgcgcaaga tcggcgcgcc gaccgtcgat cgcatctggc gcctcggttt cgcggcgcgt
     1201 ttcttcttcg ccatcctgac ccattccgga acggccttcc gtcgcctgcc gctgaccttg
     1261 cgcgaaatct acttcagtgg cgtgctatcg ctgttgatca tcctggtttc aggcttgttc
     1321 gtcggtctgg tgctcggcct tcagggctac gaaaccctgc agcgctatgg ctcgaccgag
     1381 gccctgggga ttcttgttgc actgtcgctg gtgcgcgagc tgggaccggt ggttgccggc
     1441 ctgttgttcg cctcgcgggc cggttcgtcg atcaccgccg agatcggtct gatgaagacg
     1501 accgagcagt tgaaggcgat ggacatgatg gcggtcaatc cgctggcccg tgtcgtggcg
     1561 ccgcgcttct ggggcggggt ggtgtcgatg ccgctgctgg cggcgatgtt ctcggcgatg
     1621 ggcgtgttcg gcggctggct gatcggggtc gttttcatcg gggtcgatga tggcgccttc
     1681 tggtcgcaga tgcagtcaag cgtcgatttt cgctacgaca ttctcaatgg catcatcaag
     1741 agtgtcgtct tcggttttgc tgtttcgctg attgcagtgt tcgaggggta cgacgcggtc
     1801 ccgacggcgg aaggggtgtc gcgagcgatc acccgcaccg tcgtgacttc ggcgatgacc
     1861 attctggcgc tggatttcat tctgacatcg ttcatgttcc ggggaacatc atgaaccgta
     1921 ccgtaatcga cctctgggtc ggcttcttcg ttgctctcgg cattgccgca gtgttgtttt
     1981 tggcgctcaa ggtgggcaat ctttcggcgg cccacttgtc cgagacctat gtgctgcaag
     2041 ccaagtttga taacatcggc ggcctgaagg tgcgcggtcc ggttcgtagt gccggcgttc
     2101 tggtaggcag gatcgcggat atccgcttcg atcccgaaag ctaccaggcg gtggtgacga
     2161 tgaacatcga cgggcgttac cgctttccgc gggatacctt cgcatcgatt tatacggcgg
     2221 gtctgctggg cgagcagtac atcggtttcg acgtcggtgg cgacgagaag atgctgcaac
     2281 cgggcgacac ggtgaaaatg acccagtcag cggtggtctt ggaaaaactg atcagccagt
     2341 tccttttcgg caaggcggct gaaggacagg atagcaaatg acattgaagg caggattttc
     2401 ggttcggcga ttgcttcgtc tgctggctct cggcggtgcg ctggcggtct cgaccgctca
     2461 tgccagcgcc caggaagctc agcgcgatcc ttacgaaaac ttcaatcgtt cgatgttctc
     2521 ggtgaacgaa gcgctggata aatacgccgc ccgtccggtc gcccaggcct acgatgctgc
     2581 ctttcccttg ccggtcaagg ccagtgtcgg caatttcttc ggcaatgtcg gtgaagtctg
     2641 gggcggtgcc aacaacgcgc tgcagggcaa gccggccgat gccggtctgg gactgacgcg
     2701 tttcctgatc aactcgactg tcggcatcct ggggctgttc gatgtcgcca gcgaactcgg
     2761 cttcgagcgt tacaacgagg atttcggcca gacgatggcg gtttggggcg tcggcaacgg
     2821 cccctacgtc ttcctgccgg tggttggtcc gcgcacctcg cgcgacctgc tgggctgggc
     2881 tgtcgatacc tatgtcgacc cggtgtcgac gatccacccg gaatcggtgc gcattggtac
     2941 ctcggtcctg cgtttcgtcg atgttcgcgc cggtttgctg cccgccgaca aggtggtcga
     3001 ggaagcggcg ctcgacaaat acgcctacgt gcgcgatgcc tacctgcagc gtcgccgcaa
     3061 cctgatttac gatggccgcc cgccgcgtct ggatgattga gttgagggtt ctttgatgat
     3121 caagcgtttg cttgctgtat gcctggcctg ttttctcgcc gctccgctgt ttgccgccga
     3181 agcgcccgac gcgctgattc gtcgtgtttc cgaagaagtg ctggaaatcg tccgccagga
     3241 caaggacatc cgcaacgggg acatccagaa gatcagcgcg ctggtcgacg ccaaagtgct
     3301 gccttatttc aatttccagc gcatgacctc gctggcggtc ggtcgcgact ggcgcagcgc
     3361 caacccggcc cagaagaacc gtctggccga agaattcaag aacctgctcg ttcgcacata
     3421 ctccaatgca ctgagtagct acagcaacca gcgtatcgtt ttcaagccgt tcaagatgaa
     3481 gagtggcgaa acggaagtgc tggtgcgctc cgaagtgacg caacccggtt cgcaaccggt
     3541 gcagatcgat tactggctgg aacaatctgg tgacaactgg aaggtcttcg atgttgtcgt
     3601 ggccggcatc agtctggtta ccaattaccg cgagcagttc gccaatgaag tgcgcgctgg
     3661 cggcatcgac ggcctgatcg ccagcctggc agccaagaac aagtcgctgg aagtcgctgc
     3721 ggcggcaaaa aacggcaaga aatgatcgag cgtagcaacg gtgccctcgc cgttcgcggc
     3781 gccatggtga tggcgaatgc cagggccttg ctggcggccg gctgcgccga gatggggggc
     3841 gattcggtcg tcgatctgtc cgccgtcgac gcagccgatt cctcggcgct ggcggtgatg
     3901 ctcggctggc tgcggcatgc ccaggcggcc aagcagcgcc tttcctttgt cggcgttccc
     3961 gccggcgtgc tctccctggc cgaactctac ggcatttccg acctgttgcc cctggcctga
     4021 gcggcctgtc catgcctgca gccgtttccg tcgtcgacgt cgtcaagtcc tttgcttccc
     4081 tcaaggcact ggccggaata tcgctgcgca tcgagcccgg cgaatttttt ggcctgctcg
     4141 gccccaacgg cgccggcaag acgacgctga tttcctgcct ggccgggctg acccgggtcg
     4201 acagtggttc gatcagcgtc cttggccacg atgtcgtgcg tgacttccgc caggcgcggc
     4261 ggctgctcgg cgtggtgccg caggaactgg tgttcgatcc cttcttcagt gtgcgcgaga
     4321 ccttgcgcat ccagtcgggc tacttcggta tccgcaacaa cgatgactgg atcgacgaaa
     4381 tcctgcatca cctcgatctc accagcaagg ccgacgccaa catgcggcgt ctgtcgggcg
     4441 gtatgaagcg gcgtgtcctg gttgcccagg cgctggtaca caagccgccg gtgatcgttc
     4501 tcgacgagcc gaccgccggg gtcgatgtcg aattgcgcca aggtctgtgg cagttcgtcc
     4561 gccgcctcaa cagcgagggg cacaccatca tcctgaccac gcattacctc gaggaggcgg
     4621 aagccctgtg caaccgcatc gccctgatga aacaggggca gctgattgcg ctcgacagta
     4681 cggccaacct gatggcggcg catcccggcg cttcgctgga agaggttttc atgcggacgc
     4741 tacggcaatg agcatcaatg agttgctgat cggtttctgg accctgtttt acaaggaagt
     4801 cctgcgcttc tggaaggttg ccttccagac cgtcgccgcg ccggtgctga cggcgatgct
     4861 ctacctgatg atcttcggtc atgtgctcga ggaccatgtc cgggtgcatg gcattggcta
     4921 caccagtttc ctgatccccg ggctggtgat gatgcaggtg ctgcagaacg ctttcgccaa
     4981 ttcatcgtcg agcctgatcc aggccaagat caccggttcc atcgtgttcg tcctgctgcc
     5041 gccgattccc tatggcgcgt tctttgctgc ctacgtgctg gcggctctgc tgcgcggcct
     5101 gatggtcggt gtcggcgtgc tgctggcgac cgtcgttttt g
//
LOCUS       DAOSDX010000071         5124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5780_length_5124_cov_3.808838, whole genome shotgun sequence.
ACCESSION   DAOSDX010000071 DAOSDX010000000
VERSION     DAOSDX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_5124_cov_3.808838"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLN02_02460"
     CDS             complement(<1..575)
                     /locus_tag="PLN02_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51212.1"
                     /translation="MRKQAIFPVLFGLAVLLATPALACRPFGSYQLVEDKTGGIWFTE
                     GDNNAVSRLAPDGSVKAYALPTKNAEPSALALDRRGNVWFVEMDGGKIGRLAPNGRIR
                     EFPTRDGHPGMVAVDRWGEAWFTQMSGHENDSDEHAGHGSAMIAKVGRIDRQGRMHDF
                     PLREGWPTSLAFDRRDRAWVTILVPGRNGAQP"
     gene            complement(593..2467)
                     /locus_tag="PLN02_02465"
     CDS             complement(593..2467)
                     /locus_tag="PLN02_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51213.1"
                     /translation="MLLASPAWGGEASETDVSLITAADIAREKPASLLEMLKRRVGLD
                     ENNSVISMRGVRGIAVVVDGFVSSVSELSALRPEQVERIEVLRGAASARFGAEAMGGA
                     IAVTTRRAGGRQNPALNLTQGVDSRGGNYTRLGGSGERGELAWSLLAENREDQGFRTV
                     PRSPFPYQITVADEHGRSTAVDGKLAWTGRDFAASLNLKHSDNHSFFGRPNWAFDWQT
                     DTVRSQFAWQVNERWSLEAALGEDRNATAGVRDRGSGIDAAGLAADQWLTQDTRQREA
                     SAALAWQDGGWQGRLGGSLIELSEQFSASDYASRATRTQADSLIRKEALFASGEMPLG
                     AGRLELGLRHDWQRYISSRVFDAGPPGQETRGGGVVKAATSPKIALSWPLGVTTRLRG
                     SLGSGFSPPQATQLYNGFVSAGSVTLANPALRPERSTTADLALLGSAAGGNGGVTLFA
                     TRWQDKIAVRILDYGSPVVQQPQNVGEVRAHGLETHWSQPLAGHWQLDANYTYTRTRI
                     VRDQSAPQLVGNVLPDMPRHKANFVLNYASTAGFSARAKWRLLGSAYTDEANTVVDSR
                     GYRWKKAAYDVLDLAASWRAKGWEATLALDNVFDRDYVSGFFWHGEPRTLRGEFILRF
                     "
     gene            complement(2484..4706)
                     /locus_tag="PLN02_02470"
     CDS             complement(2484..4706)
                     /locus_tag="PLN02_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51214.1"
                     /translation="MFQKAFAGERRPLLSTIATALFLAFQSSAVLAVPVDVAAQPLGS
                     ALRALASQTGVQIAHASDVVGTLQASAVKGDMRLEEALRRLLAGTGLEFRKDGDSSYV
                     IVRAGHSEKVLPEMVVTATRTERAVDEVPASVSVISSEDLRRLHLDRVEDALKRVEGI
                     DFNTSAASPFGSSPMMRGIGGSYAGVTSSVLIDGMATDSAISGIAGRGGLDFLAPQDI
                     ERIEVVRGPASALYGSNVMGGVINAIPARWRGDTGGEAHAEFGSHDTQKLGAVVGTAN
                     DRFDVRLSVYDAQSDGFKAKPKPDPWGGQDVRKRGWTDRKWNLNGAFRPTDDQEIGFS
                     YQQYRTKQNYVGGDVFINSERREGDAYTLSYLKDFSDTASLKLKYRHLDLLQDWIDSD
                     MGMGVGYRKSVSDNLDAQLDLRLSAQNTLVVGAFYQSADFKTVSLSDNYRDVSTADSY
                     GVFVQDEHRFGDLTAVLGGRFDRFSQGASYTDGAAVHRGTKENVFSPRLGLRYRLTPM
                     ASLYASAGSAFLPANADMKFHGGSARWQDNPGLKPEKSVTYEIGINLKPAWGDLRSAV
                     FHTDYRDMISTMSVGATPWPRKFVNIGKVEVDGFEFAFEGNLDGRWRPYANYAYTRSI
                     IKENPSAPQTVGKRVQRIAPHKFNFGVTYAPADAWSASFGGRYVSERYFTDNNTADRK
                     ADAYFVADAKLVVELPAFANARWNAYLAVNNLFDKKHTVWEYEYADGRSAWLGLNARF
                     "
     gene            4849..>5124
                     /locus_tag="PLN02_02475"
     CDS             4849..>5124
                     /locus_tag="PLN02_02475"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOI51215.1"
                     /translation="MNATTCPPTEPLVLDLYRRHFDELRHFLGRRLDCPEAGRDAAQE
                     IFLRLLINPPAVDIRNPRAFLLRSARNFLIDLGRAAQSRPQLVVVDET"
BASE COUNT          898 a         1702 c         1586 g          938 t
ORIGIN      
        1 ggttgggcgc cattgcgccc cggcaccagg atcgtcaccc aggcgcggtc ccggcggtca
       61 aaggccagcg aggtcggcca accttcgcgc aggggaaaat cgtgcatccg gccctggcgg
      121 tcgatgcgac cgaccttggc gatcatcgcg ctgccgtggc cggcgtgttc gtcgctgtcg
      181 ttttcatgcc cggacatctg cgtgaaccac gcttcgcccc agcgatcgac ggcgaccatg
      241 ccggggtggc cgtcgcgcgt ggggaattcc cggatgcgcc cgttcggcgc cagacggccg
      301 atcttgccgc cgtccatttc gacgaaccag acgttgcccc ggcgatccag cgccagagcg
      361 ctgggctcgg cgttcttcgt cggcagcgca taggccttga cgctaccgtc cggcgccagc
      421 cgcgacacgg cgttgttgtc gccctcggta aaccagatgc cgccggtttt gtcctcgacc
      481 agttggtagg aaccgaaagg ccggcaagcc agggccggcg tcgccagcag cacggcgagg
      541 ccgaagagaa cggggaaaat cgcttgcttg cgcatgtata actccttgcc agtcagaaac
      601 gcaaaatgaa ttcgccgcgc agggtgcgcg gctcgccatg ccagaagaaa ccgctgacgt
      661 agtcgcggtc gaagacgttg tcgagcgcca gcgttgcttc ccagcccttg gcccgccagc
      721 ttgccgccag gtcgaggacg tcgtaggccg cctttttcca gcgatagccc cggctgtcga
      781 ccacggtgtt ggcttcgtcg gtgtaggcgc tgccgagcaa gcgccatttg gcgcgggcgg
      841 aaaatcctgc ggtcgacgcg taattcagca caaaatttgc cttgtgccgg ggcatgtccg
      901 gcaggacgtt gccgaccagt tgcggcgccg actggtcgcg gacgatgcgg gtgcgggtgt
      961 aggtgtaatt ggcgtcgagc tgccaatgcc cggcgagcgg ctgcgaccaa tgcgtttcca
     1021 gcccgtgcgc tcgcacctcg ccgacgtttt gcggctgttg caccactggc gaaccgtaat
     1081 cgaggatacg cacggcaatc ttgtcctgcc agcgggtggc gaagagggtg acgccgccat
     1141 tgccacccgc cgcgctgccg agcagggcga ggtcggcggt ggtcgaacgt tccggcctca
     1201 gcgccgggtt ggccagcgtc accgagccgg cactgacaaa accgttgtac aactgtgtcg
     1261 cctgcggcgg cgaaaaaccg ctgcccaggc tgccgcgcag gcgggtggtc acgcccagcg
     1321 gccaggacag cgcgatcttc ggactggtcg ccgccttgac cacgccgccg ccgcgcgttt
     1381 cctgcccggg tgggccggcg tcgaacacgc gtgacgagat atagcgttgc cagtcgtggc
     1441 gcagccccaa ttccaggcgg ccggcgccga gcggcatttc gccggaagca aacagcgcct
     1501 ccttgcggat cagcgaatcg gcctgcgtgc gcgtggcgcg gctggcgtaa tcgctggcgc
     1561 tgaattgttc cgacagttcg atcaggctgc cgcccagccg tccctgccag ccgccgtcct
     1621 gccaggccag cgccgccgag gcctcgcgct ggcgggtatc ctgagtcagc cattggtccg
     1681 ccgccagacc ggcggcgtcg ataccgctgc cgcgatcacg cacgccggcc gtggcattgc
     1741 ggtcttcacc cagcgccgct tccagcgacc agcgttcgtt cacctgccag gcaaactggc
     1801 tgcgcacggt atcggtctgc cagtcgaagg cccagttcgg ccggccgaaa aagctatggt
     1861 tgtccgaatg cttgaggttc agcgacgcgg cgaaatcgcg cccggtccac gccagcttgc
     1921 cgtccacggc agtgctgcgg ccgtgctcgt cggcgaccgt gatctggtag ggaaaaggcg
     1981 agcgcggcac ggtgcggaaa ccttggtctt cgcggttttc cgccagcagt gaccaggcca
     2041 gttcaccccg ctcgccgctg ccacccagac gggtgtagtt gccgccccgg gaatccaccc
     2101 cctgcgtcag gttgagcgca ggattctgcc gaccgccggc ccgccgcgtc gtcaccgcaa
     2161 tcgccccgcc catcgcctca gcgccgaaac gcgccgacgc cgcgccgcgc aacacctcga
     2221 tgcgctcgac ctgctccggg cgcaaggccg acaactcgct gacggaagaa acgaagccat
     2281 cgaccaccac ggcaatgccg cgcaccccgc gcatgctgat gacgctgttg ttctcgtcca
     2341 gcccgacccg ccgcttcagc atctccagca gactggccgg cttctcgcgg gcgatgtcgg
     2401 cggcggtgat cagggagacg tcggtttctg atgcctcgcc tccccaggcc ggactggcca
     2461 acagcatcag caggaccgcc gttctagaaa cgcgcattca gcccgagcca cgcgcttcgg
     2521 ccatcggcat actcgtattc ccaaacggtg tgttttttat cgaacaaatt attcaccgcc
     2581 agataggcat tccagcgagc gttggcaaac gcaggtagtt caacgaccag cttggcatcg
     2641 gcgacgaagt acgcgtcagc cttgcggtcc gcggtgttgt tatcggtaaa gtagcgctca
     2701 ctcacatagc gcccgccaaa gctggcactc caggcatccg ccggtgcata ggtcacgccg
     2761 aagttgaact tgtgcggcgc gatacgctga acccgcttgc cgacggtttg cggcgcgctc
     2821 ggattctcct tgatgatgga gcgggtgtaa gcgtagttgg cgtaaggacg ccagcggcca
     2881 tccagattgc cttcgaaggc gaattcgaag ccgtcgacct cgaccttgcc tatattcacg
     2941 aacttccgcg gccacggggt agcccccacg gacatcgtcg aaatcatgtc ccgatagtcc
     3001 gtgtggaaga ccgcgctgcg gagatcaccc caagccggtt ttaaattgat cccgatttcg
     3061 taggtgaccg atttttcggg ttttaggcca ggattgtcct gccagcgtgc cgacccccca
     3121 tgaaatttca tgtccgcatt ggccggcagg aacgcgctac cggcggaagc atacagagaa
     3181 gccatcggcg tcagtcgata acgcaaaccc aggcggggac tgaagacgtt ttctttggtt
     3241 cctcgatgca cggccgcacc gtccgtatag gatgcccctt ggctgaagcg atcaaagcgc
     3301 ccccccagca cggcggtaag atcgccgaaa cggtgctcgt cctgcacaaa cacaccgtac
     3361 gaatcggcag tcgagacatc ccgatagttg tcggagagcg agaccgtctt gaaatccgcg
     3421 gattgataga aagcgccgac aactaaagtg ttctgcgccg atagccgaag gtcgagttgc
     3481 gcatcaaggt tatcgctgac gcttttgcga tagccaacgc ccatgcccat gtcggagtcg
     3541 atccagtcct gcaggaggtc gaggtgacga tatttcagct tcaagctggc tgtatcggag
     3601 aaatctttca ggtaagacag cgtgtaggcg tcgccttccc gtcgctcgct gttgatgaac
     3661 acgtcgccac caacgtaatt ctgtttggtt cggtattgct ggtagctaaa gccgatttcc
     3721 tgatcgtcgg tcggccggaa ggccccgttg agattccatt tcctgtctgt ccagcctctt
     3781 ttcctgacat cctgcccacc ccacggatcc ggtttcggct tggccttgaa accatccgat
     3841 tgcgcgtcat acaccgacag gcgaacgtcg aaacgatcgt tcgccgtacc aacgacggcg
     3901 cccaatttct gcgtatcgtg agagccgaat tcggcatgcg cctcgccgcc ggtgtccccc
     3961 cgccaacggg caggaatggc gttgatcacc ccacccatca cattcgagcc gtaaagcgcc
     4021 gatgccgggc cgcgcaccac ctcgatgcgc tcgatatcct gcggagcgag aaagtcgagg
     4081 cctcctcggc cggcgattcc ggatattgcc gagtcggtgg ccatcccatc gatgagcacg
     4141 gaagaggtca ccccggcata tgagccgccg ataccacgca tcatcggaga actaccgaaa
     4201 ggagaagcgg cgctggtgtt gaaatcgata ccctcgactc gcttcaaggc atcctcgacc
     4261 cggtccaggt gcaggcgtcg caggtcctcg ctggaaatca cgctgacgct cgccggcacc
     4321 tcgtccacgg cgcgctccgt ccgcgttgcc gtgaccacca tttccggcag caccttttcg
     4381 ctgtgcccgg cgcgcacgat gacgtagctg ctgtcgccat ccttgcggaa ctccagcccg
     4441 gtgccggcga gcaggcggcg cagggcttct tccaggcgca tgtcgccttt gactgcgctg
     4501 gcctggagcg tgccgacgac atcgctggcg tgggcgatct gcacgccagt ctggctggcc
     4561 agggcacgca atgcgctgcc cagcggctgg gcggcaacgt cgaccgggac ggcgaggacg
     4621 gcgctgctct ggaaggccag gaacaaggcc gtggcgatgg tgctgagaag aggccggcgc
     4681 tcgccggcga aggctttttg aaacacgtga tttccccctt ggttgtctgg cgatgcgctc
     4741 gcctctcccc accaagacgg atgaatttgc cctctcccta cctctgccgg gcctagcgcg
     4801 acaatatgcc gcatggagtt gcgcggcatc gccgattagc atgcggccat gaatgccacc
     4861 acttgtccgc ctaccgagcc gctggtactt gatctctatc gcaggcattt cgacgagttg
     4921 cggcattttc tcgggcgccg gctcgactgt ccggaggccg gccgcgatgc cgcgcaggaa
     4981 atcttcctgc gcctgctgat caatccgccg gctgtcgaca tccgcaatcc gcgcgccttc
     5041 ctgctccgct cggcacgcaa cttcctcatc gacctcggcc gcgccgccca gagccggccg
     5101 caactggtgg tggtcgatga gacg
//
LOCUS       DAOSDX010000072         5104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5812_length_5104_cov_3.108140, whole genome shotgun sequence.
ACCESSION   DAOSDX010000072 DAOSDX010000000
VERSION     DAOSDX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_5104_cov_3.108140"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..820)
                     /gene="fliP"
                     /locus_tag="PLN02_02480"
     CDS             complement(77..820)
                     /gene="fliP"
                     /locus_tag="PLN02_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236830.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOI51216.1"
                     /translation="MRNKLPLALLALTIAGPVFAQATGLPAVTSTPAGGGGTTYSLTI
                     QTLVLLTALTFIPAAVLMMTSFTRIVIVLSLLRHAMGTQTAPPNQVLVGLSLFLTFFI
                     MAPTLDRIYEEAYVPLAENRIDIMQAGERAAVPLRAFMLKQTREADIALFAGIARIDK
                     IEKPEDVPFRILVPSFVISELKTAFQIGFILFIPFLVIDMVVASILMSMGMMMMSPIM
                     VALPFKLMLFVLVDGWHLVIGSLVQSFNP"
     gene            complement(810..1229)
                     /gene="fliO"
                     /locus_tag="PLN02_02485"
     CDS             complement(810..1229)
                     /gene="fliO"
                     /locus_tag="PLN02_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOI51217.1"
                     /translation="MPRLLPISAFLPLPALAAETAAPGISSGTYVQATLALIFIVGLL
                     FLATWAARKVSGGKRFGQGNMRVISGIALGPKERIVLVEVEEQWLVIGIVPGQIRTLH
                     TLPKGVAEKASGDGQAPATPPFADWLQRIRERRDHAE"
     gene            complement(1319..1732)
                     /gene="fliN"
                     /locus_tag="PLN02_02490"
     CDS             complement(1319..1732)
                     /gene="fliN"
                     /locus_tag="PLN02_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOI51218.1"
                     /translation="MADDQISDDDWAAAMAEQATIDANGAAAQPAAQPADIFPSFAAP
                     PATGGVMNELDMILDIPVQITVELGRTKITIKNLLQLAHGSVVELDAMAGEPMDVLVN
                     GTLIAQGEVVVVNDKFGIRLTDIITPSERMRKINR"
     gene            complement(1752..2765)
                     /gene="fliM"
                     /locus_tag="PLN02_02495"
     CDS             complement(1752..2765)
                     /gene="fliM"
                     /locus_tag="PLN02_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOI51219.1"
                     /translation="MAGDFLSQDEVDALLKGVTGETDDPEEGGGDESGIRAYNLGTQE
                     RIVRGRMPTLELVNERFARYLRIGLFNYMHRNAEISVGPIRVQKYSEFIRNLVVPTNL
                     NLVAAKPLRGTALFVFDPNLVFLVVDNMFGGDGRFHTRVEGRDFTATEQRIIQGLLGV
                     VFAEYSKAWKPVHTIQFEYIRSEMNSQFANIATPSEIVVSTSFTLEFGGATADMHICF
                     PYSMLEPIRDLLYSTMQSDQLSTDKRWISTLRRQLQGAEVEIVAQLGSGKISLREILR
                     LKPGDVIPITIPASIEALVDNVPLMECSYGQQNGQYALKVEKFIASPPELSGDPAKGD
                     KNV"
     gene            complement(2772..3341)
                     /locus_tag="PLN02_02500"
     CDS             complement(2772..3341)
                     /locus_tag="PLN02_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOI51220.1"
                     /translation="MAKDAKPAEEGSEAPPKKSKKLIIIIVAVVLLLVIAGGAAALLL
                     KKDADAEAGDEEVAEETEKPAKKAAKKKDHGAPPVFVNLDAFTVNLVPETGDQYLQVV
                     LSLELDNPEAQAQLTQLMPRIRNNVTLLLSSKKASELLPKEGKEKLAGEIMNEINSAI
                     EPPTKNKKGEVVHAEGPVISVLFTSFIIQ"
     gene            complement(3378..>5104)
                     /locus_tag="PLN02_02505"
     CDS             complement(3378..>5104)
                     /locus_tag="PLN02_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOI51221.1"
                     /translation="PAPTESSSAKRSAPQDALAAAASALDAITPRNAAPIDGAAESTT
                     RIAPSNNAPRTPAAPSTPIVDSGRFTDQLALASTRTAAPTAAQGDSQANRQASTGEQT
                     LTTEIPAATSATTPSVSNDNNARGIARKPASVTPATQPAQQTQQNMAEKPAPTQRKAI
                     DQAFTSDRRNLATASANAPANASADPLASATRAASPTQPATTSSDTPLPVNNQARGIA
                     TGIERMPETPSAPTPAFEFASAQRPAPRPLPSAQPAVSSQPTPDVDTAPEDPTPVLAK
                     TTQNQVQTSSAPLPSVPLSSATAAPAIVTAATTESLSGRRAAIRNTVQESRGTPPSPL
                     AAASDAGDEAFAVPQTGQAIRGAASASPVAASQAQSAAIAVTTDVKPENTDGSSVPSD
                     SAKLAAMPAAPANTAPPAGLAVSGEASAAQHPETTLQLATHLRDPRWGEQLGERVVVL
                     AQGEKNSSAQIDINPAQLGPIRVKIDMNGDQVSVQFSSASSEVRQVLEDALPRLREQL
                     ANSGIQLGQANVGSQSQQTPREAFAQSTPSPRSPGEGAILPAESPLEAQSSGRLIQSG
                     RGLVDLFA"
BASE COUNT          786 a         1486 c         1800 g         1032 t
ORIGIN      
        1 gccaggagca gcggcgccga cagcatcaat gccgcctcga tggcctggcg gccgatttcc
       61 atcacggtcc ccggcgtcat gggttgaagc tctggaccag cgagccgatg accagatgcc
      121 agccgtcgac gaggacgaac agcatcagct tgaacggcag ggcgaccatg atcggcgaca
      181 tcatcatcat ccccatcgac atcaggatgc tggcgacgac catgtcgatg accaggaagg
      241 ggatgaagag aatgaagccg atctggaacg cggtcttcag ttcgctgatg acgaatgagg
      301 gcaccaggat gcggaagggc acatcttccg gtttttcgat cttgtcgatg cgggcgatgc
      361 cggcgaacag cgcgatgtcg gcctcccgcg tctgcttgag catgaaagca cgcaggggaa
      421 cggcggcgcg ttcgccggcc tgcatgatgt cgatgcggtt ctcggcgagc ggtacgtagg
      481 cttcctcgta gatgcggtcg agggtcggcg ccatgatgaa gaaggtcagg aacagcgaca
      541 gcccgaccag gacctggttg ggcggcgcgg tctgggtacc catcgcgtgg cgcagcagcg
      601 aaaggacgat gacgatgcgg gtgaagctgg tcatcatcag cacagcggcg ggaatgaagg
      661 tcagcgccgt cagcaggacc agcgtctgga tggtcaggct gtaagtcgtg ccgccgccac
      721 cggccggcgt gctggtgacc gccggcaggc cggtggcttg ggcgaatacc gggccggcga
      781 tagtcagcgc gagcagggcc aggggcagct tattccgcat ggtcgcgtcg ctcgcggatg
      841 cgttgcagcc agtcggcgaa aggcggcgtg gcaggcgctt ggccatcacc cgaggccttt
      901 tcggcaacgc ctttgggcag ggtgtgcagg gtccggatct ggccggggac gatgccgatg
      961 accagccact gctcctcgac ttcaacgagg acgatacgct ccttggggcc gagcgcgatg
     1021 ccgctgatca ccctcatgtt gccctggccg aaacgcttgc cgccggaaac cttgcgtgcc
     1081 gcccaggtgg cgaggaacag gaggccgacg atgaagatca gcgccagcgt cgcctgcacg
     1141 taagtgccgc tggaaatgcc gggggcggca gtttccgcgg cgagcgccgg tagtggcaga
     1201 aaagcggaaa tcgggagaag gcgcggcaag gcggcaggcg gcaggtaagg aaagtgcgct
     1261 atcttaaacc caaaaccccg gccgctggct ttctgacggt gcagccgggg gcgccggctc
     1321 agcggttgat cttgcgcatg cgctccgaag gcgtgatgat gtcggtcagc cggatgccga
     1381 acttgtcatt gaccaccacc acttcgccct gggcaatcaa ggtgccattg accaggacat
     1441 ccatcggttc gccggccatc gcgtcgagtt cgacgaccga gccatgggcg agctgcagca
     1501 ggttcttgat ggtgatcttg gtgcggccga gttcgacggt gatctggacc gggatatcca
     1561 ggatcatgtc gagttcgttc atgacgccgc cggttgccgg tggcgcggcg aaggaaggaa
     1621 agatatcggc cggttgcgcc gcgggctggg cggccgcccc gttggcatcg atggtggcct
     1681 gctcggccat ggcggcggcc cagtcgtcgt cgctgatctg atcgtcggcc atgccggggt
     1741 cctgcatttc ttcagacatt cttgtctccc ttggcggggt cgccgctcaa ttcggggggt
     1801 gaggctatga atttttcgac cttcagggcg tactgcccgt tctgctggcc gtaactgcat
     1861 tccatcaacg gaacattgtc caccagtgct tcgatgctgg ccgggatggt gatgggaatg
     1921 acgtcgccgg gcttcaggcg gaggatctcg cgcaacgaaa tcttgcccga gccaagctgg
     1981 gcgacgattt ccacttcggc tccctgcaat tgtctgcgca aggtactgat ccagcgcttg
     2041 tcggtcgata gctggtcgct ctgcatcgtg ctgtagagca ggtcgcggat cggctcgagc
     2101 atcgagtacg ggaaacagat gtgcatgtcg gcggtggcgc cgccgaattc cagcgtgaac
     2161 gaggtcgaga caacgatttc cgatggcgtc gcgatgttgg cgaactgcga gttcatctcc
     2221 gagcggatgt actcgaactg gatggtgtgc accggcttcc acgccttcga atattcggcg
     2281 aagacgacgc ccagcaagcc ctggatgatg cgttgttcgg tggcggtgaa atcacgcccc
     2341 tcgacccggg tatggaagcg gccgtcgccg ccgaacatgt tgtcgacgac caggaagacc
     2401 agattggggt cgaagacgaa cagcgccgtg ccgcgcagcg gcttggccgc gaccaggttc
     2461 aggttggtcg ggacgaccag gttgcggatg aactcgctgt atttctgcac gcggatcgga
     2521 ccgaccgaga tttccgcgtt gcggtgcatg tagttgaaga ggccgatgcg caggtaacgg
     2581 gcgaaacgct cgttgaccag ttccagcgtc ggcatccggc cgcggacgat gcgctcctgg
     2641 gtgcccaggt tgtaggcgcg aatcccggat tcgtccccgc cgccttcttc cggatcgtcg
     2701 gtttccccag tgacgccctt gagcagggcg tcaacttcgt cctgggagag aaagtcgcca
     2761 gccatggctc cttactggat gatgaacgag gtgaacagca ccgagattac cgggccttcg
     2821 gcgtgaacga cttcgccctt cttgttcttg gtcggcggct cgatcgccga attgatttcg
     2881 ttcatgatct cgccagccag cttttccttg ccttccttgg gtagcagttc ggaagccttc
     2941 ttgctcgaca gcagcagggt cacgttgttg cggatgcgcg gcatcagttg cgttagttgc
     3001 gcctgcgctt ccggattgtc gagttccagc gagaggacga cttgcaggta ctggtcgccg
     3061 gtttccggga ccaggttgac cgtgaaggcg tcaaggttga cgaagaccgg cggcgcgccg
     3121 tgatccttct tcttggccgc cttcttcgcc ggcttctcgg tttcttccgc cacttcctcg
     3181 tcgcccgcct cggcgtcggc atccttcttc agcagaagcg ccgcggcgcc gccggcgatg
     3241 accagcagca agacgaccgc cacgatgatg atgatcagtt tcttgctttt cttcggtggg
     3301 gcttcgctgc cctcttcggc tggcttcgca tccttcgcca tgattctccc tctcctttta
     3361 ccctcgggtc atcttcttta ggcgaacagg tcaaccagac cacgtccgct ctggatcaag
     3421 cgaccggagg attgtgcctc cagcggcgat tcagcgggaa gtatagcgcc ttcgccaggg
     3481 gaacggggcg agggtgtgga ttgggcaaaa gcctcgcgcg gcgtttgctg cgactgcgag
     3541 ccgacgttgg cctggccaag ctggatgccc gagttcgcca gctgttcgcg cagacgcggc
     3601 aaggcgtctt ccagtacctg gcggacttcc gacgaggcgc tggagaactg tacggagacc
     3661 tggtcgccgt tcatgtcgat cttgacccgg atcggcccga gctgggccgg gttgatatcg
     3721 atctgcgccg acgagttctt ttctccctgg gccaggacca cgacacgttc gccaagctgt
     3781 tcgccccagc gcggatcgcg cagatgggtg gctagctgga gggtggtttc gggatgctgc
     3841 gctgcgctcg cttcgccact cacggcaagt ccagccgggg gcgcggtatt tgccggcgct
     3901 gccggcatgg cggcaagttt tgccgagtca ctcgggacgc tggagccgtc cgtgttctcc
     3961 ggtttgacgt cggtcgttac cgcgatcgca gcgctttgtg cttggcttgc ggcgactggc
     4021 gatgcgctgg cggcgccacg gatcgcttgc ccggtttggg gcacggcgaa ggcttcatcg
     4081 ccagcatcgc ttgccgcggc aagcggggaa ggaggcgtgc cgcgactttc ctgaacggtg
     4141 ttgcggatcg ctgctcggcg cccggacagg gattcggtgg tggcagccgt cacgatggcc
     4201 ggtgccgccg tcgcgctgct caacgggacg ctagggagcg gtgccgacga tgtctggacc
     4261 tggttctgcg tggttttggc gagaacgggt gtcgggtctt ccggcgcggt atcgacgtcc
     4321 ggcgtcggct gcgaactgac agcaggctgg gcgctgggga gcgggcgcgg cgcagggcgc
     4381 tgcgcgcttg caaactcgaa cgcaggcgtc ggcgcgctcg gcgtctctgg catgcgctcg
     4441 atcccggtgg caatgcctct cgcctggttg ttgacgggca agggagtgtc gctgctggtc
     4501 gtcgccggct gggtcggact tgccgcgcgg gtggccgatg ccagcgggtc ggccgatgcg
     4561 tttgccgggg cgtttgccga ggctgtggcg agatttcgcc ggtcgctggt gaaagcctgg
     4621 tcaatggcct tgcgctgggt cggcgctggc ttttccgcca tgttttgttg cgtttgctgc
     4681 gcgggctgcg tggcgggcgt gacgctggca ggtttccgtg caatgcctcg ggcgttgttg
     4741 tcgttgctca cgctcggggt tgtggcgctc gttgcggcgg gtatctcggt agtcagtgtc
     4801 tgctcgccgg tgctggcctg acggtttgcc tggctgtcgc cttgtgccgc ggtcggggcg
     4861 gcagtgcggg tgcttgccag ggcgagttgg tcggtgaagc gcccgctatc gacaatcggc
     4921 gttgacgggg cggcaggggt gcggggagcg ttgttcgaag gcgcgatgcg cgtcgtgctt
     4981 tcggccgcgc cgtcgatcgg cgcagcgttt ctcggcgtga tcgcgtcgag ggcggaggcg
     5041 gcggcagcca gggcgtcttg cggggcgctc cgctttgcgc tgctgctctc tgtcggcgcg
     5101 ggtt
//
LOCUS       DAOSDX010000073         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5855_length_5081_cov_2.900915, whole genome shotgun sequence.
ACCESSION   DAOSDX010000073 DAOSDX010000000
VERSION     DAOSDX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5855_length_5081_cov_2.900915"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLN02_02510"
     CDS             complement(<1..508)
                     /locus_tag="PLN02_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51222.1"
                     /translation="MLRSSLRIVISLILAAVVWWIGPMVAIGRYHPFGWLWLRQLLVV
                     LLLFWGFYPLLRWLLGKFLSLFRRRKQKAPTVDNDRISSRLADLELTLRKIWVDRQKG
                     VFRRLSYRLRKGHLREQPWFMLLGSSGAGKTSFLNHTGQNALPGEVAGLPRLGESLGQ
                     TEDCGFWVN"
     gene            complement(618..2057)
                     /gene="icmH"
                     /locus_tag="PLN02_02515"
     CDS             complement(618..2057)
                     /gene="icmH"
                     /locus_tag="PLN02_02515"
                     /inference="COORDINATES: protein motif:HMM:NF021377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HOI51223.1"
                     /translation="MRGINEIMSALRNIPTSGGVSSMGALGDIAQRISSSMPRGADNP
                     QPFGNLLEHSDDTPALAAPVEQPASKEKPVDRSVGAVAMLGMRNAIARPGVAASKWQN
                     PYISAAIPPLLLLHRLEDQGITFDTSLRGQLGLEVRMFREKLASSGEESRWIDDASYL
                     LCTHIDELLNEACRKQDLPLPEKSLLVEFHGDAWGGEDCFIKLETAMSQERPPIALLE
                     LYEVILGLGFQGRYQVIDRGDVLLQDLRSKLHRMIWDRAPDGLALRSGLPKVFKPRWL
                     TPFRLFLIGAALLSIAYLAGVFDIAMRGSPLREALAAYEPPVREINISETLPPPLPEL
                     IAEGWLTAERRENGWLLIFRADGAFEPGKAEIRPEFLPNLTRLGAAFAPWPGDLEVIG
                     HTDLTPILASKFPSNQALSEARAREVAKLIEQQVAGGKAQRQVTASGMGEKAPVIAEI
                     NPAANLKNRRVEVLWKVAATRRVVTEIIR"
     gene            complement(2054..3415)
                     /gene="tssK"
                     /locus_tag="PLN02_02520"
     CDS             complement(2054..3415)
                     /gene="tssK"
                     /locus_tag="PLN02_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HOI51224.1"
                     /translation="MATHNINRVVWTDGLLVEAQHFQQQERYIEHQVNSRLSQITNLG
                     WGFSELEIDQDLLQQGLLAIIRARGIFDDGTPFSIPTCDAIPEALDLSDAPIGTRICL
                     ATVRSDVVNSSVGFGEAEAAARYKVVEAEVQDANVGLDPEGSPLRIHMQVGQLRTRLC
                     REDHVGASETWQPIARLAARQGNRGIVLDSDFIPAMLDVRAHTRLLAVCNEFQGLLQH
                     RLSASAGQRLLASGGGLADLIEMLATQAISEYRMRLDHLEAYDPLHPADLYLELTGLL
                     GRLSVLPSVEPMLTAQKWRYQHDDLQACFSALAHALRRALSLIIESPVIPLRFEDRGD
                     QVYLCINDPQWKLQKMVFAFTSAMPGENLRRMLPTQVKFGPVEHINKLVDLQLPGARL
                     VPVSAVPRNLPFYAQSVYFEVESVDPYWKETMRGAAMALRVVGDFPQLKFEAWGLREG
                     KVA"
     gene            complement(3430..3957)
                     /locus_tag="PLN02_02525"
     CDS             complement(3430..3957)
                     /locus_tag="PLN02_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51225.1"
                     /translation="MSWKAVLIAACLAAGIQGCTIGDATRVLFRGMPDRLIPEKRNIS
                     LTIHGGPKLNAKFEGDVARPLRLCIYLHKQEDWNPPMDLSAPCAEQDSRVSKAQKITL
                     LADRVAYITESVPYGDAYWITVTGDFADQGGNGKGILKVQSPADLNSCHWLNIDRNQI
                     QEVKKNSAGNQPTCK"
     gene            complement(3962..4345)
                     /locus_tag="PLN02_02530"
     CDS             complement(3962..4345)
                     /locus_tag="PLN02_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HOI51226.1"
                     /translation="MFANSSAGGIDISPADVCKTPPLCIPIPYPNLAFGSMAIPNVPN
                     ILYCGGPVHNLATIIPVTFCDEPGACGGIISGTVKGLSIHCTAKLNFLVGGAPITRMT
                     SINLPNTLNTLGVRLVPSQLKILVI"
     gene            complement(4412..4897)
                     /locus_tag="PLN02_02535"
     CDS             complement(4412..4897)
                     /locus_tag="PLN02_02535"
                     /inference="COORDINATES: protein motif:HMM:NF023482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3540 domain-containing protein"
                     /protein_id="HOI51227.1"
                     /translation="MPGSSYMTRIRRGEQGWQLESDTNTPVRVAVSCLVEPLDGDQVL
                     VASINGVCYVLAVLERDWQQGQRIDVDLGGGELHLKAANIVVEVERNLDLQAENFRLN
                     AELSVQNASTQVVNVAGISVLNANSIALDGKQSLTMHTSLGAIQASALLKIDGTQMHF
                     G"
BASE COUNT          930 a         1587 c         1635 g          929 t
ORIGIN      
        1 cgttgaccca gaagccgcaa tcctcggtct gccccagcga ttcgccgagg cgcggcaggc
       61 cggccacttc gccgggcaag gcattctggc cggtgtggtt gaggaaactg gtcttccccg
      121 ctcccgacga accgagcagc atgaaccagg gctgttcgcg cagatgcccc ttgcgcaggc
      181 gatagctcag gcgacggaag acgcctttct ggcgatcgac ccagatcttg cgcagggtca
      241 gctcgaggtc ggcgagacgg ctgctgatgc ggtcgttgtc caccgtcggc gccttctgct
      301 tgcggcggcg gaagagcgac aggaacttgc cgagcagcca gcgcagcaag ggatagaagc
      361 cccagaacag caacagcacg accagcagct ggcgcagcca cagccagccg aacggatggt
      421 agcggccgat ggcgaccatc ggtccgatcc accagacgac ggcggcaaga atcaaggaga
      481 tgacgatgcg taacgaactt ctcaacattt ctgatgctcc caacccaggg tttcgccagt
      541 gcaaacccct gcgctgatca acttcgatga accccggtct tttccggacc ggtaattgct
      601 gcctaccctc ttcccgttca gcggatgatt tcagtcacca cgcgccgggt tgccgccacc
      661 ttccacaaga cctcgacccg acggttcttg aggttggccg ccggattgat ttcggcgatc
      721 accggtgcct tctcgcccat cccggacgcc gtcacctgac gctgtgcctt gccgccggca
      781 acctgctgct cgatcagctt ggcgacttcg cgggcacggg cttcggaaag cgcctgattg
      841 gaggggaact tgcttgccag gatcggcgtc aggtcggtgt gaccgatgac ttccagatca
      901 ccgggccagg gcgcgaaagc ggcgccgagg cgggtcaggt tgggcaggaa ctcgggccgg
      961 atttccgcct tgccgggctc gaatgccccg tcggcgcgga agatcagcag ccagccgttt
     1021 tcccgccgct cggccgtcag ccagccttcg gcgatcagct ccggcagcgg cggcggcagg
     1081 gtttccgaga tgttgatttc gcgcaccggc ggttcgtagg cggccagcgc ctcgcgcagc
     1141 gggctgccgc gcatggcgat gtcgaaaaca ccggccaggt aggcgatcga cagcagcgcg
     1201 gcgccgatca ggaagagccg gaacggcgtc agccagcgcg gcttgaacac cttgggcaaa
     1261 cccgagcgca gggccaggcc gtcgggcgcg cgatcccaga tcatgcggtg cagcttgctg
     1321 cgcaaatcct gcagcaacac gtcgccgcga tcgatgacct ggtagcgccc ctggaagccg
     1381 aggccgagga tcacctcgta cagttcgagc agcgcgatcg gcggccgttc ctggctcatc
     1441 gccgtttcca gcttgatgaa gcagtcctcg ccgccccagg cgtcgccgtg aaattcgacg
     1501 agcagggatt tttccggcaa gggcagatcc tgcttgcggc aggcttcgtt gagcaactca
     1561 tcgatatgcg tgcacaacag gtaggaggcg tcgtcgatcc agcgcgattc ttcgccgctg
     1621 ctcgccagct tctcgcggaa catccgtact tccagcccga gctgtccgcg caacgaggta
     1681 tcgaaggtga tgccctggtc ttccagacga tgcagcagca ggagcggcgg gatcgccgcc
     1741 gagatgtagg gattctgcca cttcgacgca gccacgccgg gccgggcgat agcgttgcgc
     1801 atgccgagca tggcgaccgc cccgaccgat cgatcgaccg gtttttcctt gctcgccggt
     1861 tgctcgaccg gcgccgcgag ggccggcgtg tcgtcgctgt gttcaagcag gttgccgaag
     1921 ggctggggat tgtcggcgcc gcgcggcatc gagctggaaa tgcgctgggc gatgtcgccc
     1981 agcgcgccca tcgacgaaac accaccggag gtcgggatgt tgcgcagcgc gctcatgatt
     2041 tcattgatgc ccctcatgcc actttccctt cacgcagccc ccaggcctcg aacttcagtt
     2101 gcgggaaatc gccgaccacg cgcagcgcca ttgccgcgcc gcgcatcgtt tccttccagt
     2161 agggatcgac cgattcgacc tcgaaataga cgctctgcgc atagaaaggc agattgcgcg
     2221 gcaccgccga caccggcacc aggcgcgcgc ccggcagttg cagatcgacc agcttgttga
     2281 tgtgctcgac cgggccgaac ttgacctggg tcggcagcat gcggcgcagg ttctcgccgg
     2341 gcatcgccga agtgaaagca aagaccatct tctgcagctt ccactgcgga tcgttgatgc
     2401 acaggtagac ctggtcgccg cgatcttcga agcgcagcgg gatcaccggc gattcgatga
     2461 tcaacgacag ggcgcggcgc aacgcatggg cgagcgcgct gaaacaggct tgcaggtcgt
     2521 cgtgctggta gcgccacttc tgcgcggtca acatcggctc gacgctgggc agcacggaaa
     2581 ggcggccgag caagccggtc aattcaagat agaggtcggc cggatgcagg ggatcgtagg
     2641 cttcgagatg atcaaggcgc atccggtatt cggagatcgc ctgcgtcgcc agcatctcga
     2701 tcaggtcggc caggccgccg ccgctggcga gcaggcgctg gccggcggaa gcgctgaggc
     2761 ggtgctggag cagcccctgg aactcgttgc agacggccag cagacgggta tgggcgcgaa
     2821 cgtcgagcat cgccgggatg aaatcgctat cgaggacaat gccgcggttg ccctggcgcg
     2881 ccgccaggcg ggcgatcggt tgccaggttt cgctggcgcc gacgtgatcc tcgcggcaca
     2941 ggcgggtgcg cagctggccg acctgcatgt ggatgcgcag cggcgaccct tccgggtcga
     3001 gaccgacgtt ggcgtcctgc acttcggctt cgaccacctt gtaacgggcg gcggcttcgg
     3061 cctcgccaaa gccgaccgaa ctattgacca cgtcgctgcg cacggtggcg agacagatcc
     3121 gggtgccgat cggcgcatcc gaaagatcaa gcgcttccgg aatggcgtcg caggtcggaa
     3181 tcgagaacgg cgtaccgtcg tcgaaaatgc cgcgcgcccg gatgatggcg agcaagccct
     3241 gttgcagcag atcctggtcg atttcgagtt cgctgaatcc ccaaccgagg ttggtgatct
     3301 gtgacaggcg gctgttcacc tggtgttcga tgtagcgttc ctgttgttga aaatgctgcg
     3361 cttcaaccag caagccatcg gtccagacca ccctgttgat attgtgtgtt gccatgaaga
     3421 agtgaacccc tatttgcatg tgggctgatt gcccgcactg tttttcttga cttcctgaat
     3481 ctggttgcgg tcgatgttca gccagtggca actattgaga tcggccggcg actggacctt
     3541 gagaatgccc ttgccattcc ctccctggtc ggcaaaatca ccagttaccg tgatccagta
     3601 ggcgtccccg tagggcaccg attccgtgat gtaggcgacg cggtcggcaa gcaaggtgat
     3661 cttctgcgcc ttgctcaccc gggaatcctg ttcggcgcag ggcgcgctca gatccatcgg
     3721 cggattccag tcttcttgct tgtgcagata gatgcacagg cgcagcggcc gcgcgacgtc
     3781 gccctcgaac ttggcgttga gcttggggcc gccgtggatg gtcagcgaga tgttccgttt
     3841 ctctgggatc agccggtccg gcatccccct gaacagcacc cgcgtcgcgt cgccgatcgt
     3901 gcagccctgg atgcccgctg cgagacaggc agcaatcagt acagctttcc aggacatggg
     3961 atcagatgac cagaatcttc aactgactgg gcaccaggcg gacgcccagc gtattcaagg
     4021 tgttcggcag gttgatgctg gtcatccggg tgatcggcgc gccgccgacc aggaagttca
     4081 gcttggccgt gcaatggatg gaaagcccct tgacggtacc ggagatgatg ccgccgcagg
     4141 cgccgggttc atcgcagaag gtgaccggga tgatggtcgc caggttatgc accgggccgc
     4201 cgcaatagag gatgttcggc acgttgggaa tggccatcga accgaatgcc aggttgggat
     4261 agggaatcgg aatgcacaag ggcggcgtct tgcagacatc ggccggagaa atatcgatac
     4321 cacctgcgga gctattggca aacatacgag cctcttccta aaacaatagt gaacatccca
     4381 agcaacagtc caacccgccg cttccccaaa atcacccgaa atgcatctgg gtgccgtcga
     4441 tcttcaacaa tgcgctggcc tggatcgcgc ccagactggt gtgcatggtc aggctctgct
     4501 tgccgtcgag ggcgatcgag ttggcattga gcacgctgat cccggcgaca ttgaccacct
     4561 gcgtcgatgc gttctgcacc gacagttcgg cgttgagccg gaagttctcg gcctgcagat
     4621 cgagattgcg ctcgacctcg acgacgatgt tggccgcctt caggtgcagt tcgccgcccc
     4681 ccagatcgac gtcgatccgc tgcccctgct gccagtcccg ttcgagcacg gcgaggacgt
     4741 agcagacgcc gttgatgctg gcgaccagca cctggtcgcc atcgagcggc tcgaccaggc
     4801 aactgacggc gacccgcacc ggggtattgg tatcgctttc gagctgccag ccctgttcac
     4861 cgcgacggat tcgcgtcatg taactggagc cgggcatcag gctcggatag ttggcagccg
     4921 ccggccgttg gctgaccggg gcgacgggag cttgtgtaac gggttccagc aatttcattg
     4981 ggtactcctg gtagccccgg ggcggcaggt cttccaccac tcgaggtcct gcagctcttc
     5041 gggaatttca aaacgcgcca gatcgaggtc ggcgccgcgc c
//
LOCUS       DAOSDX010000074         5016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5948_length_5016_cov_2.719815, whole genome shotgun sequence.
ACCESSION   DAOSDX010000074 DAOSDX010000000
VERSION     DAOSDX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5948_length_5016_cov_2.719815"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLN02_02540"
     CDS             <1..291
                     /locus_tag="PLN02_02540"
                     /inference="COORDINATES: protein motif:HMM:NF022609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HOI51228.1"
                     /translation="DGAAFVAHTGAPAGEIRTVLIDGDGSLYLDAALGIGLLDDRDLA
                     AFFEECRPHDEVSLLRLMAGNGGEIRWQGLPLQYIAADRLAQRYAYLKQPRP"
     gene            complement(298..501)
                     /locus_tag="PLN02_02545"
     CDS             complement(298..501)
                     /locus_tag="PLN02_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51229.1"
                     /translation="MTHDDIRAAIRSGLPFFGVTRQGQVMARYLPFGPVYRWKRNQMI
                     PAPLQGEDLLWWLQASDDDPGEP"
     gene            649..1023
                     /locus_tag="PLN02_02550"
     CDS             649..1023
                     /locus_tag="PLN02_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51230.1"
                     /translation="MTPAEIATWASSKLVAPLDIDCVITLMLKILDGKCKMSDADQLV
                     ASQLYDAVGQRPVHRLDAASCHALIAESRRHCDENLKMRIYEQRLLAETMLSRPVMKA
                     FKARLREAGILYHDSADRSTAA"
     gene            1098..1547
                     /locus_tag="PLN02_02555"
     CDS             1098..1547
                     /locus_tag="PLN02_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51231.1"
                     /translation="MPKRPHLTTIARSFSAAVVLLALVACTPRLLAPYDEVTDRSVSA
                     LQRQTEAHLIGLEAAAGTPDCVFDRHQRFYTDARIEISALAVRTAAIPGNERTAEQTT
                     LLLASLDSLRQLHRLGCLAPEQIAILRTQFNSSFTAILRLELAKRRP"
     gene            1562..1891
                     /locus_tag="PLN02_02560"
     CDS             1562..1891
                     /locus_tag="PLN02_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51232.1"
                     /translation="MKLDTGELSQAILAAFQGSLHERWPEIREYGESEAKKLAQTLVM
                     IEALQASGKITPAQAALHLEIQKNACRSVLLTLEGLGLLAVEAALDAALDVVRETVNG
                     ALGFALL"
     gene            1917..3404
                     /locus_tag="PLN02_02565"
     CDS             1917..3404
                     /locus_tag="PLN02_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51233.1"
                     /translation="MAKTMAPYYPIIYVRGYAMTPGEIADAVSMPYMGFNQGATKLRQ
                     AWDGRVTKHIFESPLIRLMKEYGYRDCFADGAEISGSLPARSVVIYRYYETADSDLGD
                     GKTLSIPEAARGLQALIQRVRRQVCGDDDELLAAFKVHLVAHSMGGLVIRAFLQNDAI
                     STPADRALVAKVFTYGTPHNGIEMAGINVPSVLGLWDINNFNRQRMAEYLGLPGKPER
                     VDSLDGKFDPQRFFCFVGTNHRDYAAALGVSRLLAGEMSDGLVKIENATVSGAPRAFA
                     YRSHSGPFGVVNSEEGYQNLVRFLFGDLRVDGSLDVDVLPLPPSVQKAKDAGKQIRGS
                     YYFEATVAPRGSFSAKLTERRRDTYSAVLRRYDELLRVDRAGRDAPRSPALFSTFLDS
                     KRITVGKTLVFSVELAVSSTGYTIDDSFWLDQHIEGEYLFRDTLTLKLTLGQDGFRLR
                     YLSSDENWSEGQGRLVDPTGDQYLIPLTSSKGFRGQLRLNVSRSG"
     gene            complement(3409..4284)
                     /locus_tag="PLN02_02570"
     CDS             complement(3409..4284)
                     /locus_tag="PLN02_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HOI51234.1"
                     /translation="MSDGRRVRVTGAASGIGAQDVACADGPVAFHHSQAWQALARHPA
                     LDWGRTLFAPNGERLSRTARIAALCRTLADEVETTLRQGQFPLVIGGDHSVAVGTWSG
                     VARGIGQPLGLLWIDAHLDSHTPESSYSGAVHGMPLACLLGQGDRRLLGIGLPGAQVS
                     AGHCVVLGARSYEPEEIDFLCRQGVRIIDGEEIARRGFAACLDEAVGIVASAPAGFGV
                     SLDLDAIDPRFAPGVGSPEPDGLPAADVRTAWRWLAGLPGLRALEIVEYNPDRDRHGM
                     TAQLIADLIGETVAG"
     gene            complement(4289..4654)
                     /locus_tag="PLN02_02575"
     CDS             complement(4289..4654)
                     /locus_tag="PLN02_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI51235.1"
                     /translation="MTADPLAFSPEMDVLKAVHLLLEHQLSGAPVLDGDGHLLGFFSE
                     KDGLKVALNASYYEQPGGPVGHYMTREVLTLSASSSMADAIELFVAHHYRCYPVLDDG
                     RLVGQLCRRDALTALEKLW"
     gene            complement(4663..4893)
                     /gene="nqrM"
                     /locus_tag="PLN02_02580"
     CDS             complement(4663..4893)
                     /gene="nqrM"
                     /locus_tag="PLN02_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HOI51236.1"
                     /translation="MQTFLVTFGIIAFLVLIMAIGVMFGRKPIAGSCGGYKALNLDCA
                     AGCKEPCEKRLARLQAGTEAQAEEESCSGRCS"
BASE COUNT          919 a         1760 c         1545 g          792 t
ORIGIN      
        1 gacggcgcgg ccttcgtcgc ccataccggt gccccggccg gtgagatcag gaccgtgttg
       61 atcgacggcg acggcagcct ctaccttgac gcggcgctcg gcatcggttt gctcgacgac
      121 cgcgacctgg ccgctttttt cgaggaatgc cggccccatg acgaggtcag cctgctgcgc
      181 ctgatggcgg gcaacggcgg cgaaatccgc tggcagggcc tgccgctgca atacatcgcg
      241 gccgatcggc tggcccagcg ctacgcttat ctcaaacaac cacgcccgta agcgcgctca
      301 gggctcgccc ggatcgtcgt cgctggcctg cagccaccac aacagatcct cgccctgcaa
      361 tggcgccgga atcatctggt tgcgcttcca gcgatagacc ggcccgaacg gcaggtaacg
      421 cgccatcacc tggccctgcc gggtgacgcc gaaaaaaggc aggccggaac ggatcgcggc
      481 gcggatatcg tcgtgtgtca tgcgtctgtc acctttttta caaacgcccc gagtatcccg
      541 cgcctgccgc tgccggacaa gtcccaaacg gcgcgaacgg cacgggcacc cgggcgactg
      601 gccctgttct tgctgtgtct ctggcgccaa tcactaccac acaacgccat gacccctgct
      661 gaaatcgcca cctgggcaag cagcaagctc gtcgccccgc tcgatatcga ctgcgtcatc
      721 accctgatgc tcaagatact cgacggcaaa tgcaaaatga gcgacgccga ccagctggtc
      781 gccagccagc tctacgacgc agtcggccag cggccggtgc accgccttga tgccgccagt
      841 tgtcacgcgc tgatcgccga gtcgcgtcgg cattgtgacg aaaacctgaa aatgcgcatt
      901 tacgaacagc gcctgctggc cgaaaccatg ctctctcgcc cggtgatgaa ggctttcaag
      961 gcacgcctgc gcgaggccgg catcttgtac cacgacagtg ccgaccgctc cacagcggcc
     1021 tagggccttg ccttcgtcat gacgcggtat atgatgcaga caagtccatc gtcgatcacc
     1081 gccagccggg agcctgcatg ccgaagcgac cgcatctcac caccattgcc cgctcatttt
     1141 cggccgccgt cgtactactg gcgctggtcg cctgtacacc gcgcctgctg gcgccttacg
     1201 atgaagtcac cgaccgcagc gtcagcgccc tgcagcggca gaccgaagcc cacctgatcg
     1261 gccttgaggc ggctgccggc acgcccgatt gcgttttcga caggcaccag cgcttctata
     1321 ccgacgcccg cattgaaatc agcgcccttg ccgtgcgcac cgcagccatc cccggcaacg
     1381 agcgcaccgc cgaacagacg acactgctcc tcgccagcct cgacagcctg cgccagttgc
     1441 accggctcgg ctgtctggcg ccggaacaga tcgccatcct gcgcacccag ttcaacagca
     1501 gcttcaccgc catcctcagg ctggaactgg cgaagcggcg cccatgacca cggagccaac
     1561 aatgaagctc gacaccggag aattgagcca ggccatcctg gccgccttcc agggcagcct
     1621 gcacgagcgc tggccggaaa tccgcgagta cggcgagagc gaggcgaaga agctggcgca
     1681 gacgctggtc atgatcgaag ccttgcaggc gtcgggcaag atcacgccgg cccaggccgc
     1741 gctgcatctg gaaatccaga agaacgcctg ccgcagcgtt ttactgacgc tcgaaggact
     1801 cggcctgctc gccgtcgaag ccgcgctcga cgcggcgttg gacgtcgtcc gcgaaacggt
     1861 caacggggcg cttggcttcg ccctgctgta agcccatacc acccgaggag acgatcatgg
     1921 caaagacaat ggcaccgtac tacccgatca tctatgtccg cggctacgcg atgacgcccg
     1981 gcgaaatcgc cgatgccgtg tcgatgccgt acatgggctt caaccagggc gcgaccaagt
     2041 tgcgccaggc ctgggatggg cgcgtcacca agcacatctt cgagtcgccg ctgatccggc
     2101 tgatgaagga atacggctat cgcgactgct tcgccgacgg cgccgagatc agcggcagcc
     2161 tgccggcgcg cagcgtcgtc atctatcgct attacgagac ggcggacagc gacctcggcg
     2221 acggcaagac gctgtcgatt cccgaagcgg cgcgcggcct gcaggccttg atccagcgcg
     2281 tgcgccgtca ggtctgcggc gacgacgacg aactgctcgc cgccttcaag gtgcatctgg
     2341 tcgcccattc gatgggcgga ctggtgatcc gcgccttcct gcagaacgat gcgatcagca
     2401 ccccggccga ccgcgctctg gtcgccaagg tgttcaccta cggcacgccg cacaacggga
     2461 tcgagatggc gggaatcaac gtgccgtcag ttctcggcct gtgggacatc aacaacttca
     2521 accggcagcg gatggccgaa tacctcggcc tgcccgggaa gcccgagcgg gtcgactcgc
     2581 tggacgggaa attcgacccg cagcgcttct tctgcttcgt cggcaccaat caccgcgact
     2641 acgccgccgc gctcggcgtt tcccgcctgt tggcgggcga gatgagcgac ggcctggtga
     2701 agatcgagaa cgccaccgtc agcggtgccc cgcgcgcctt cgcctatcgc agccacagtg
     2761 gcccgttcgg cgtcgtcaat tcggaagagg gttaccagaa cctggtccgt ttcctgttcg
     2821 gcgacctgcg cgtcgatggc agtctcgacg tcgacgtcct acccctgccg ccctcggtgc
     2881 agaaggccaa ggacgccggc aagcagatcc gcggctccta ctatttcgag gcgacggtcg
     2941 cgccacgcgg cagcttcagc gccaaactga ccgagcgccg ccgcgacacc tacagcgccg
     3001 tgctgcgccg ctacgacgaa ctgctgcgcg tcgaccgtgc cggccgcgac gcgccacgct
     3061 cgcccgccct gttctcgacc ttcctcgaca gcaagcgcat caccgtcggc aagaccctgg
     3121 tcttctcggt tgaactggcg gtgtcaagca ccggctacac catcgacgac agcttctggc
     3181 tcgaccagca catcgaaggc gaatacctgt tccgcgacac cctgacgctg aagctgacgc
     3241 tgggacagga cggcttccgg ctgcgctacc tgagctcgga cgagaactgg agcgagggcc
     3301 agggacgact ggtcgacccc acgggcgacc agtacctgat cccgctcacc tcgagcaagg
     3361 gattccgcgg tcagctccgt ctgaacgtca gccgttccgg ctgagccctc aaccggccac
     3421 cgtctcgccg atcaggtcgg cgatcagttg cgcggtcatg ccgtggcggt cgcgatccgg
     3481 gttgtactcg acgatttcca gggcgcgcag gccgggcaag ccggccagcc agcgccaggc
     3541 cgtgcgtacg tcggccgccg gcaagccgtc cggctcggga ctaccgacgc ccggcgcgaa
     3601 gcgcggatcg atggcgtcga gatcgaggct gacgccgaaa ccggccggcg cgctggcgac
     3661 gataccgacc gcctcgtcga ggcaggcggc gaagccgcgg cgggcgattt cctcgccgtc
     3721 gatgatgcgc acgccctggc gacacaggaa atcgatttct tccggctcgt agctgcgcgc
     3781 cccgagcacg acgcaatgcc cggcgctgac ctgcgcgccg ggcaggccga tgcccagcag
     3841 acggcggtcg ccctggccga gcaggcaggc cagcggcatg ccatggacag cgccggaata
     3901 gctgctttcc ggcgtatggc tgtcgaggtg ggcgtcgatc cacagcaagc ccaatggctg
     3961 cccgatgccg cgggcgacgc cgctccaggt gccaaccgcc accgaatggt cgccgccgat
     4021 gaccagcgga aactgcccct ggcgcagggt ggtttcgacc tcgtcggcga gcgtccggca
     4081 gagggcggcg atccgcgccg tgcgcgacag ccgctcgccg ttcggggcaa acagggtgcg
     4141 tccccaatcg agcgccgggt gccgggccag cgcctgccag gcctgcgaat gatgaaaggc
     4201 aaccggcccg tcggcacaag ccacatcctg ggcgccgata ccgctggcgg cgccggtcac
     4261 gcgcacccga cggccgtcgc tcatcggcct accagagctt ttccagcgcc gtcagcgcat
     4321 cgcgccggca caactgaccg accaggcgcc cgtcatcgag caccggataa cagcggtagt
     4381 gatgcgccac gaacagttcg atggcatcgg ccatcgacga cgacgccgac agggtcagca
     4441 cctcgcgggt catgtaatgg ccgaccgggc cgcccggctg ttcgtagtag gaggcgttga
     4501 gcgccacctt gaggccgtcc ttttcggaaa agaaaccgag caggtgacca tcgccatcaa
     4561 gcaccggcgc accactgagc tggtgttcga gcagcaggtg caccgccttc agcacgtcca
     4621 tctccgggct gaaagccagg ggatcggcgg tcatgtaatc ctttacgagc aacgacctga
     4681 gcatgactct tcctccgctt gtgcctcagt cccggcctgc aggcgggcca ggcgtttttc
     4741 acagggttcc ttacagccgg ccgcacaatc caggttgagc gccttgtaac cgccgcaact
     4801 gccggcgatc ggcttgcgcc cgaacatgac accgatcgcc atgatcagga ccaggaaggc
     4861 gatgattccg aaagtcacca gaaatgtctg catggcatcc tcccttcccg ctcagccgcc
     4921 gaaatcgtcg agcatgatgt tctcgcgctc gacgccgaga tcgagcaaca tcttgatcac
     4981 cgcggcgttc atcatcggtg gcccgcacat gtagaa
//
LOCUS       DAOSDX010000075         5010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5959_length_5010_cov_2.994147, whole genome shotgun sequence.
ACCESSION   DAOSDX010000075 DAOSDX010000000
VERSION     DAOSDX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5959_length_5010_cov_2.994147"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLN02_02585"
     CDS             <1..946
                     /locus_tag="PLN02_02585"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HOI51237.1"
                     /translation="QTGLRHLCLAGGVALNCVSNGRLLREGPFKEIWIQPAAGDAGGA
                     IGCALAAYYAKSGAARVPEAGDSMQGSYLGPRYATDEIRRQLDEYGAVYRELDDNTFF
                     DQVSDVLADEKVVGWFQGRMEFGPRALGGRSIIGDPRSRNMQSVMNLKIKYRESFRPF
                     APSVRAEKVGEWFEQESASPYMLIVAPVREDKRIAMTAEEEKLFGIDKLNVPRSQIPA
                     VTHVDYSARIQTIHRQTNPRYYRLIEAFEAKTGCPVLINTSFNVRGEPIVCTPEDAYR
                     CFMRTEMDTLVIENFVLAKSDQPAWEGDESWKDEFQLD"
     gene            969..1376
                     /locus_tag="PLN02_02590"
     CDS             969..1376
                     /locus_tag="PLN02_02590"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOI51238.1"
                     /translation="MFHEIPVLDRKGLRDFGLLTGALFAGIFGLFLPWHFGWAWPLWP
                     WLILAVLGGLALVAPLALNPFYKLWMRFGMVMGAIMSRLILGIVFFLVVTPLGLLMRA
                     TGKDPMHRRFDRQAASYRELIGANKSNSLDKPF"
     gene            1385..1537
                     /locus_tag="PLN02_02595"
     CDS             1385..1537
                     /locus_tag="PLN02_02595"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOI51239.1"
                     /translation="MLELLKDLWAFMQVRKKYWLAPVIIVLLLLGGLLVLSQGSAIAP
                     FIYTLF"
     gene            complement(1539..2306)
                     /locus_tag="PLN02_02600"
     CDS             complement(1539..2306)
                     /locus_tag="PLN02_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOI51240.1"
                     /translation="MIDWQVNPDYATGEAGTAFASLDAVFALQGERVTVDPESEVIRL
                     RLDGILYYVKRYTIGRRKLARRWFGLRDLFGPQRAVKEWRNLQRFHAWGLPTATLVAW
                     GQERRGGRFVRGALVTEELRDTVDLAQLAKRDDPRLRDRAWVAVVSTQVAACTRAMHA
                     HGFVHNDLKWRNLLVTGGDAPQLYLIDCPNGGFWMQPFLGYRIVKDLACLDKVAKYAL
                     SRTQRLRFYLDYVQRQRLTAADKRRLRRILAFFAGRE"
     gene            complement(2303..3127)
                     /gene="rfaP"
                     /locus_tag="PLN02_02605"
     CDS             complement(2303..3127)
                     /gene="rfaP"
                     /locus_tag="PLN02_02605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HOI51241.1"
                     /translation="MRDELRLAPPFDRLWAGQDAFAAVDRLEGEVFRELDGRRTFRTE
                     VEGRGYFVKIHRGVGWGEILKNLVSLRLPVLGAGNEWRAIRRLEELGVDTMRAVAYGR
                     RGANPAREHSFIVTEELAPTVSLEDFCREWPKMPPAPALKRALIRRVATMVGKMHRGG
                     VNHRDCYLCHFLLHLDPAPSAAALKLSLIDLHRAQIRAQTPRRWRDKDLAGLYFSALE
                     IGLTQRDRLRFLRDYFARPLREILRDEQALLAHLHAEGERLLAKYRRKYVPDGGRA"
     gene            complement(3112..4245)
                     /locus_tag="PLN02_02610"
     CDS             complement(3112..4245)
                     /locus_tag="PLN02_02610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI51242.1"
                     /translation="MQLAFCLYKYFPFGGLQRDFLRIALACQARGHAIRVYTLSWQGE
                     VPAGFEVVTVPVRALTNARRYQKFSAWVGRDLAQRPAERVVGFNKMPGLDVYFAADPC
                     YEEKAQTLRNPLYRFSGRYRHFAAYERAVFAPESRTEILMISQVQQPFFEKHYATPKS
                     RFHLLLPGIARDRRAPPEAPAIRAAFRAEFGLADDDLLILQIGSGFKTKGLDRSLQAL
                     AHLPADLRGRTRLIAIGQDEPSQFQRMAAGLGLAGQVNILAGRDDIPRFLLGADLLVH
                     PAYNENTGTVLLEALVAGLPVLCSAVCGYAHYIDEAAGGRVIPEPFAQATLDALLREM
                     LVDRAQRAVWQRNALAWAESADIYSNAERAAEIILKDGPCATN"
     gene            complement(4315..>5010)
                     /locus_tag="PLN02_02615"
     CDS             complement(4315..>5010)
                     /locus_tag="PLN02_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOI51243.1"
                     /translation="STSQPVIARPALAAVFAQARQLFGHSDVHIRHILGKLLMLHLIQ
                     LDEDRDGAKCVVFSPGDIVAKARKAGEADPDNGKLEYEYISVDDFAAMVDLDRNRVLQ
                     KLARNELTEDLFTFRKSEVIRLLDEKGKKFFQERRMKAPEEFCDISDIEFADANSIQA
                     VLSRIDSYDLGKLLHLVEEDAVRDRILGNLPRTKRAEIESEKFDRVDEIEVRLIGEGI
                     IKAIQEKMLARRS"
BASE COUNT          843 a         1680 c         1658 g          829 t
ORIGIN      
        1 ccagaccggc ctgcggcacc tgtgcctggc cggcggcgtg gcgctgaact gcgtgtccaa
       61 cggccgcctg ctgcgtgaag ggccgttcaa ggaaatctgg attcagccgg cggcgggcga
      121 cgccggcggt gccatcggct gcgcgctggc cgcctactat gcgaagagcg gcgctgcgcg
      181 tgtgccggaa gccggcgaca gcatgcaggg cagctacctc ggcccgcgct acgcgaccga
      241 cgaaatccgc cgccagctcg acgagtacgg cgccgtctac cgggaactcg acgacaacac
      301 cttcttcgac caggtttccg acgtcctcgc cgacgagaag gtggtcggct ggttccaggg
      361 gcgcatggag ttcggcccgc gcgccctcgg cggccgttcg atcatcggcg acccgcgcag
      421 ccgcaacatg cagtcggtga tgaacctgaa gatcaagtac cgcgaatcct tccgcccctt
      481 cgccccgtcg gtgcgcgccg agaaggtcgg cgaatggttc gagcaggaaa gcgccagccc
      541 ctacatgctg atcgtcgcgc cggtgcgcga ggacaagcgg atcgcgatga ctgcggaaga
      601 agagaagctg ttcgggatcg acaagctgaa cgtgccgcgc tcgcagattc cggcggtcac
      661 ccacgtcgat tactcggcgc gcatccagac catccaccgc cagaccaacc cgcgctacta
      721 ccggctgatc gaagccttcg aggcgaagac cggctgtccg gtactgatca acacctcgtt
      781 caacgtgcgc ggcgaaccca tcgtctgtac gccggaggac gcctaccgct gcttcatgcg
      841 caccgaaatg gacaccctgg tgatcgagaa cttcgtcctc gccaagtccg accagcccgc
      901 ctgggaaggc gacgaaagct ggaaggacga gttccagctc gactgacgcg cgccccggag
      961 aatcgaccat gttccatgaa atccccgtcc tcgaccgcaa gggcctgcgc gacttcggcc
     1021 tgctcaccgg cgcgctgttc gccggcatct tcggcctttt cctgccctgg cacttcggct
     1081 gggcctggcc gctgtggccg tggctgatcc tggccgtgct cggtggcctg gcgctcgtcg
     1141 caccgctggc gctcaacccc ttctacaagc tgtggatgcg cttcggcatg gtgatgggcg
     1201 cgatcatgtc gcgcctcatt ctcggcatcg tcttcttcct cgttgtcacg ccgctcggcc
     1261 tgctcatgcg agccaccggc aaggacccga tgcaccggcg cttcgaccgc caggccgcga
     1321 gctaccgcga gctgatcggc gccaacaaat ccaattccct cgacaaacct ttctaggcgg
     1381 caacatgctc gaacttctga aagacctctg ggccttcatg caggtccgca agaaatactg
     1441 gctggcgccg gtcatcatcg tccttctgct gctcggcggc ctgctcgtgc tgtcgcaggg
     1501 ctcggccatc gcccccttca tctacacgct gttctgacct actcgcggcc tgcgaagaag
     1561 gccaggatgc ggcgcagccg ccgcttgtcg gcggcggtca ggcgctgccg ctggacgtag
     1621 tcgaggtaga agcgcagccg ctgcgtgcgg ctcagcgcgt acttggcgac cttgtcgaga
     1681 caggcgaggt ccttgacgat gcggtagccg agaaaaggct gcatccagaa accgccgttc
     1741 gggcagtcga tcaggtaaag ctgcggcgca tcgccgccag tgaccagcag gttgcgccac
     1801 ttcaggtcgt tgtgcacgaa gccgtgcgcg tgcatggcgc gcgtgcaggc ggcgacctgc
     1861 gtcgacacga ccgccaccca ggctcggtcg cgcaagcgcg ggtcgtcacg tttcgccagt
     1921 tgcgccaggt caaccgtgtc gcgcagctcc tcggtgacca gcgcgccacg cacgaagcgc
     1981 ccgccacgcc gttcctgccc ccaggcaacc agcgtcgcgg tcggcagccc ccaagcgtgg
     2041 aagcgctgca ggttgcgcca ttccttgact gcccgctgcg gcccgaagag gtcgcgcagg
     2101 ccgaaccagc gccgcgccag cttgcgccgg ccgatggtgt agcgcttgac gtagtagagg
     2161 atgccgtcaa ggcgcaggcg gatgacttcg gactcgggat cgaccgtcac ccgctcgccc
     2221 tgcagcgcga acaccgcgtc gagcgaggcg aaagcggtgc cggcctcgcc cgtcgcatag
     2281 tcgggattga cctgccagtc gatcacgccc tgcccccgtc cggcacgtat ttgcgccggt
     2341 atttcgcaag caagcgctca ccttctgcat gcaggtgggc gagcagcgcc tgttcgtcgc
     2401 gcagtatctc gcgcaagggc cgggcgaagt agtcgcgcag gaagcgcagc cggtcgcgtt
     2461 gcgtcaggcc gatttccagc gccgagaagt agagcccggc gaggtccttg tcgcgccagc
     2521 ggcgcggcgt ctgcgcgcgg atctgggcgc ggtgcaggtc gatcagcgag agcttcagcg
     2581 cggcggcgct cggcgccggg tcgaggtgga gcaggaagtg gcagaggtag cagtcgcggt
     2641 gattgacgcc gccgcggtgc atcttgccta ccatcgtggc aacgcggcgg atcagcgccc
     2701 gcttgagcgc cggggccggc ggcatctttg gccactcgcg gcagaaatct tccaggctga
     2761 cggtcggcgc cagttcctcg gtgacgatga acgaatgttc gcgcgccggg ttggcgccgc
     2821 gccggccgta ggcgacggcg cgcatggtgt cgacgccgag ttcctccagg cgacggatcg
     2881 cccgccattc gttgccggcg ccgagcaccg gcaggcgcag cgagacgaga ttcttgagaa
     2941 tctcgcccca gccaacgccg cgatgaatct tgacgaaata gccgcgccct tcgacttcgg
     3001 tgcggaaggt gcggcgcccg tcgagctcgc ggaacacctc gccttccagc cggtcgaccg
     3061 cagcaaacgc atcctgcccg gcccacaggc ggtcgaaggg cggcgccagc ctcagttcgt
     3121 cgcgcacggg ccgtccttca ggatgatctc ggcagcgcgc tcggcattgc tgtagatgtc
     3181 ggcgctttcc gcccaggcca gcgcgttgcg ctgccagacg gcgcgttgcg cgcggtcgac
     3241 cagcatttcc cggagcagag cgtccagcgt cgcctgggcg aaaggctccg ggatcacccg
     3301 accgcccgcc gcttcgtcga tgtagtgcgc gtaaccgcag accgccgagc agagcaccgg
     3361 cagcccggcg accagcgctt cgagcagcac cgtgccggtg ttctcgttgt aggccggatg
     3421 gaccagcagg tcggcgccga gcaaaaagcg cggaatgtcg tcgcggccgg ccaggatatt
     3481 gacctgcccg gccagcccga ggccagcggc catgcgctgg aactggctcg gctcgtcctg
     3541 accgatggcg atcagccggg tgcgcccgcg caaatcggcc ggcagatgcg ccaacgcctg
     3601 cagcgagcgg tccagcccct tggtcttgaa gccggagccg atctgcagga ttagcaggtc
     3661 gtcgtcggca aggccgaatt cggcgcggaa ggcggcgcgg atcgccggcg cctcgggcgg
     3721 cgcccggcgg tcgcgggcga tgcccggcag cagcaggtgg aaacgcgact tcggcgtcgc
     3781 gtagtgcttt tcgaaaaacg gctgctgcac ctgcgagatc atcaggattt cggtccggct
     3841 ctccggcgca aacaccgccc gttcatacgc cgcgaaatgc cggtagcggc cggagaagcg
     3901 gtagagcggg ttgcgcagcg tctgcgcctt ctcttcatag caggggtcgg cggcgaaata
     3961 gacgtcaagc ccgggcatct tgttgaagcc gacgacgcgc tctgccggcc gctgcgccag
     4021 atcgcggccg acccaggcag agaatttctg gtagcggcgg gcgttggtca gcgcccgcac
     4081 cggcaccgtg accacctcga aaccggccgg cacctcgccc tgccaggaca aggtgtaaac
     4141 ccggatggcg tggccgcgcg cctggcaagc cagggcgatg cgcagaaaat cgcgctgcag
     4201 gccaccgaag gggaagtatt tgtagaggca gaaagccagt tgcatgggca gggtcgccgc
     4261 ataagtcagg cggcggattt tactggcaaa cccgcacctg ggtcacgccg gcggtcagct
     4321 gcggcgggcc agcatctttt cctggatcgc cttgatgatg ccctcaccaa tcagccgcac
     4381 ctcgatttcg tcgacgcggt cgaacttttc gctctcgatc tcggcgcgct tggtgcgcgg
     4441 caggttgccg agaatgcggt cgcgcactgc atcctcctca acgaggtgga gcagcttgcc
     4501 gaggtcgtag gaatcgatgc gtgagaggac ggcctggatt gaattggcat cggcgaactc
     4561 gatgtcgctg atgtcgcaga attcttccgg tgccttcatc cggcgctcct ggaagaattt
     4621 cttgcccttc tcgtcgagca ggcggatcac ttccgacttg cggaaggtga acaggtcctc
     4681 ggtcagctca ttgcgcgcca gtttctgcag gacgcgattg cggtcgagat cgaccatcgc
     4741 cgcaaaatca tcgacactga tgtactcata ctccagcttg ccgttgtcgg gatcggcctc
     4801 acccgccttg cgcgccttgg cgacgatgtc gccgggcgag aaaacgacac acttggcccc
     4861 gtcgcgatcc tcgtcgagct ggatcaggtg caacatcagc agtttgccca ggatgtgccg
     4921 gatatgcacg tcggaatgcc cgaacaactg gcgcgcctgg gcaaaaaccg cagccagcgc
     4981 cggccgcgcg atgaccggct gcgaggtgct
//
LOCUS       DAOSDX010000076         4993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5978_length_4993_cov_3.205549, whole genome shotgun sequence.
ACCESSION   DAOSDX010000076 DAOSDX010000000
VERSION     DAOSDX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_4993_cov_3.205549"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLN02_02620"
     CDS             complement(<1..1134)
                     /locus_tag="PLN02_02620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI51244.1"
                     /translation="MTRVRTAVFDHIAEVPAAAWDALGAGHSLVYSRRFWEILEHSGI
                     NDFRYRYILFSDAADQPIGIAGFYSITTDLAIFAPPALRKLLTQLRRLFPNFLKFSIL
                     ECGTPVTPSSDPFVLAPDAPGAAVCKALDEVLQDIARRDGQLLIVMRDFEGAAWTHQA
                     DLETLGYHWLESMPNTYVEVTWPTTEAYIAAMRSHYRRKLLRYLAQGREQQFRYEIRD
                     DFADLAETLHRQWQVVHEQADEYQREVLTPAFYRELSQRLAGKVKVMLFYRQDALVGH
                     ILLMLDGDLMRFMFFGRQQAAHDGLWIYGGYALIETAIRLGIPRIEFGFTTYDLKQEL
                     GATISPVRMALRTARRILNPFVGPVFHLLNRPPEVAVRHVFRKP"
     gene            complement(1215..1682)
                     /locus_tag="PLN02_02625"
     CDS             complement(1215..1682)
                     /locus_tag="PLN02_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HOI51245.1"
                     /translation="MKLRHLAFLLLFSATAQAEEIGCVTTAWKLIGANHRVCVYAFDD
                     PKIPGVTCHVSQAKAGGIKGSLGLAEDPSQFSIACRQVGPVALPADLPESEIVFSENT
                     SLLFKETAIHRSYDAKRKVLIYLAISRRLIEGAPANAISTVPVMPWGSVAERH"
     gene            complement(1811..3121)
                     /gene="aceA"
                     /locus_tag="PLN02_02630"
     CDS             complement(1811..3121)
                     /gene="aceA"
                     /locus_tag="PLN02_02630"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HOI51246.1"
                     /translation="MSTREQQIAALEKDWAENPRWKGVKRGYSAADVVRLRGSLPIEY
                     TLAKRGAENLWAKVNGGAKKGYVNAFGAITAGQAMQQAKAGLEAVYLSGWQVAADGNT
                     SETMYPDQSLYAYDSVPTMVRRINNTFKRADEIQWSRGINPGDEGFIDYFLPIVADAE
                     AGFGGVLNAFELMKNMIASGAAGVHFEDQLAAAKKCGHMGGKVLVPTREAVEKLISAR
                     FAADVMGVSTLILARTDAEAANLLTSDYDENDKPFLTGERTQEGFYRVKNGLEQSISR
                     GVAYAPYADLVWCETGVPDLGFAREFAQAVQAACPGKLLAYNCSPSFNWKKNLDDATI
                     AKFQQGLADLGYKYQFITLAGIHINWYNTFKFAHAYARGEGMKHYVEMVQEPEFAARE
                     QGYTFVSHQQEVGTGYFDDVTTVIQGGSSSVKALTGSTEEEQFH"
     gene            3432..>4993
                     /locus_tag="PLN02_02635"
     CDS             3432..>4993
                     /locus_tag="PLN02_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI51247.1"
                     /translation="MEFLKRLYDWNERTFWNSLTKKLMSFLLLFFIDLGYLAIYFDQQ
                     RTVAETLKASAASAEAMQRIADSLDHGLILMITLTVVALSWNLLQILYIRYLILRPVR
                     MIAQIFDEIARGEGDFSRNLPTITHDELRTLAEAYNRFADKMREIISEVRKMSVNIAR
                     EAVVVKRSVASTAERANQQGEIANSVFGASSEAVQAIQEVSSSADLISHSTDSNLATA
                     RHSLAEMQEIVAKVQGVSDQLNNFNNTVGHLAERSDSIRQIAGLIKEIADQTNLLALN
                     AAIEAARAGEMGRGFAVVADEVRKLAERVNKATEEISSNIGGMIDLVRETQRENEVIN
                     GDIRQAREVVERSSGDFQQMVGNFERTGDQLTQIAAAMEELSATNSLVHDSVRQVHDL
                     SVEVVGSMKDSEQTSQMLSKATESVQELVSRFKIGRGAFDFNVDKARLFRDRVQDKLA
                     QLAASGVNVWDQDYRPQPETNPQKYLVSYCTVFEQQMQPLFEEALGSLRGGVYALIVD
                     ARGYGAIHNRKYS"
BASE COUNT          951 a         1384 c         1672 g          986 t
ORIGIN      
        1 gggcttgcgg aagacgtgcc ggactgcgac ctcaggcggc cggttgagga ggtggaagac
       61 cgggccgacg aaggggttga ggatgcggcg cgcggtgcgt agggccatgc gcaccggcga
      121 gatggtggca cccagttcct gtttcaggtc gtaggtggtg aagccgaatt cgatgcgcgg
      181 aatgcctagc cggattgccg tctcgatcag tgcgtagccg ccatagatcc atagaccgtc
      241 gtgggcggcc tgctggcggc cgaagaacat gaaacgcata aggtcgccgt cgagcatcag
      301 caggatatga ccgaccagcg cgtcctggcg gtagaagagc atgactttca cttttcccgc
      361 gagacgttgc gagagttccc ggtagaacgc cggggtcagc acttcgcgct ggtattcgtc
      421 agcctgctca tggacgacct gccattgccg gtgcagggtc tcggcaaggt cggcgaaatc
      481 gtcgcgaatc tcgtaacgga actgctgttc ccggccctgg gcaaggtagc gcaggagctt
      541 gcgccgataa tggctgcgca tggcggcgat atacgcctcc gtcgtcggcc aggtgacctc
      601 gacgtaggtg ttgggcatgc tctccagcca gtggtaaccg agggtttcga ggtcggcttg
      661 atgcgtccag gctgcccctt cgaagtcgcg catgacgatg agcagctgtc cgtcgcgccg
      721 ggcgatgtcc tgcaagacct cgtccagtgc cttgcagacc gcggcgccag gtgcgtcggg
      781 cgccagcacg aagggatcgc tgcttggggt gaccggcgtg ccgcattcga ggatgctgaa
      841 cttgaggaaa ttcggaaaca gccgacgcaa ctgggtcagc agcttgcgca gggcgggcgg
      901 cgcgaagatc gccagatcgg tggtgatcga gtagaagccg gcgatgccga tcggctggtc
      961 ggccgcatcg gagaacagga tgtagcggta gcggaagtcg ttgattccgg agtgttccag
     1021 gatttcccag aagcggcgcg aatagaccag cgaatggccg gcgccgaggg catcccaggc
     1081 cgccgccgga acctcggcga tgtggtcgaa tacggctgtt ctcacacggg tcatgggttt
     1141 gcttcagtgc gttgcggttt tacgacgcga tgatccggag catagtcgat gcggcccgga
     1201 atcccaagct tgcgtcaatg ccgctccgcg acgctgcccc agggcatcac gggcacagtc
     1261 gatatcgcgt tggccggcgc cccctcgatc aggcgccggc tgatcgccag gtagatgagc
     1321 accttgcgct tcgcgtcgta gctgcgatgg atggcggttt ccttgaagag cagtgaggtg
     1381 ttctcgctga agacgatttc gctttcgggc aggtcggcgg gcagggcgac cgggccgacc
     1441 tggcgacagg cgatggagaa ttgcgacggg tcttcggcca gccccaggct gcccttgatg
     1501 ccgcccgcct tggcctggct gacatggcag gtgacgccgg gaatcttcgg gtcgtcgaag
     1561 gcatagacgc agaccctgtg gttggcgccg atcagcttcc aggcggtggt gacgcaaccg
     1621 atttcctcgg cctgggcagt ggcggaaaac agcagcagaa aggccagatg gcgaagtttc
     1681 attctcatgt ttccgttaag gatggtcggc gaacagcata gcaagcggcc atcccggcgg
     1741 ccaacgaaaa aaaggccggc cccttgtggg agccggcctt tgccgccgcg ggatggtgcg
     1801 gctgtgcttc ttagtggaac tgttcttctt cggtcgagcc ggtcagcgcc ttgaccgagg
     1861 acgagccgcc ctggatcacg gtggtgacgt cgtcgaagta accggtgccg acttcctgct
     1921 ggtgcgaaac gaaggtgtag ccctgttcgc gagcggcgaa ttccggctcc tgaaccatct
     1981 ccacgtagtg cttcatgcct tcgccgcgag cgtaggcgtg cgcgaacttg aaggtgttgt
     2041 accagttgat gtggatgccg gccagggtga tgaactggta cttgtagccg agatcggcca
     2101 ggccttgctg gaacttggcg atggtcgcat cgtccaggtt cttcttccag ttgaaggacg
     2161 gcgagcagtt gtaggcgagc agcttgcccg ggcaggcggc ctgcacggct tgggcgaatt
     2221 cgcgggcgaa gccgaggtca ggcacgccgg tttcgcacca gactaggtcg gcgtaaggcg
     2281 cgtaggcaac accgcgggag atcgactgct cgaggccgtt cttgacgcgg tagaaacctt
     2341 cctgggtgcg ttcgccggtc aggaagggct tgtcgttttc gtcgtaatcg gaggtcagca
     2401 ggttggcggc ttcggcatcg gtacgggcca ggatcagcgt cgacacgccc atgacgtcgg
     2461 cggcgaaacg ggcagagatc agcttctcga cggcttcgcg ggtcggcacc agcaccttgc
     2521 cgcccatgtg gccgcacttc ttggcggcag ccagctggtc ttcgaagtgc accccggcgg
     2581 cgccggaggc aatcatgttc ttcatcaatt cgaaggcgtt cagcacgcca ccaaagccgg
     2641 cttcggcgtc ggcaacgatc ggcaggaagt agtcgatgaa gccttcgtca cccgggttga
     2701 tgccacgcga ccactggatt tcgtcagcgc gcttgaaggt gttgttgatg cggcgaacca
     2761 tcgtcggcac cgagtcgtag gcgtatagcg actggtccgg gtacatggtt tccgaggtgt
     2821 tgccgtcggc ggcaacctgc cagccggaca gatagaccgc ttccaggccg gccttggcct
     2881 gttgcatggc ctgaccggcg gtgatggcgc cgaaggcatt gacgtagccc ttcttggcgc
     2941 cgccgttgac cttggcccac aggttttcgg cgccgcgctt ggcgagcgtg tattcgatcg
     3001 gcagggagcc gcgcagacgg accacgtcag cagcggagta gccgcgcttg acgcccttcc
     3061 aacgggggtt ttcagcccag tccttttcca gggcggcgat ttgctgttcg cgagtgctca
     3121 tgtctgttcc tcagtagttg ggttgggaaa tcgggttgtt gcgccgggac ggaaactgtc
     3181 gggcgatgga agaagtatag agaaattcgt gcatggcagc aatggtctta tataagacat
     3241 aagataaaaa attttatttt aaaaacaatg gattgttgag tattttttgc aatacgaaac
     3301 gccattcgcg ccggtgaaat gcgctgcaac aaattggggc tgggtcgttc gcgtaggcgg
     3361 ctcaacattg tggcttgact gcaacgagaa ataggcgttg aattcgtcaa atttactaaa
     3421 tttggttgca tatggaattt ttaaaacgcc tctacgactg gaacgagcgc actttctgga
     3481 attctctgac caagaagttg atgagtttcc tgctgctctt tttcatcgat cttggctatc
     3541 tggcgatcta tttcgaccag cagcggacgg tagccgagac cttgaaggcg tccgcggcct
     3601 cggcggaagc gatgcagcgc atcgccgaca gtctcgacca cggcctgatc ctgatgatca
     3661 cgctgacggt tgtcgccttg agctggaatt tgctgcagat tctctacatc cgttacctga
     3721 tcctgcgccc ggtccgcatg atcgcccaga tcttcgacga gatcgcccgc ggcgaggggg
     3781 atttctcgcg caacctgccg accatcacgc acgatgaatt gcgcacgctg gccgaggctt
     3841 acaaccgctt cgccgacaag atgcgcgaaa tcatcagcga ggtgcgcaag atgagcgtca
     3901 acattgctcg cgaggcggtg gtggtgaaac gctcggtggc gtctaccgcc gagcgcgcca
     3961 accagcaggg tgaaatcgcc aactcggtgt tcggcgcgag cagcgaggcg gtgcaggcaa
     4021 tccaggaggt gtcgagttcg gccgatctga tttcgcattc gaccgacagc aacctggcga
     4081 ccgcccgcca ttcgctggcc gaaatgcagg aaatcgtcgc caaggtgcag ggggtcagcg
     4141 atcagctcaa caatttcaac aataccgtcg gccatcttgc cgagcgttcc gacagcatcc
     4201 ggcagattgc cgggctgatc aaggagattg ccgaccagac caacctgctg gcgctcaacg
     4261 cggcgatcga ggcggcgcgg gccggcgaga tggggcgggg gttcgcggtg gtggccgacg
     4321 aggtgcgcaa gctggcggag cgcgtgaaca aggcgaccga ggaaatttcc agcaacatcg
     4381 gcggcatgat cgatctggtt cgggaaactc agcgcgagaa cgaggtcatc aacggcgaca
     4441 tccgtcaggc gcgcgaagtg gtcgaacgct cgtccggcga tttccagcag atggtcggca
     4501 atttcgagcg taccggcgac cagttgacgc agattgcggc ggcgatggag gagttgtcgg
     4561 cgaccaattc cctggttcat gactcggtcc gccaagtgca tgacctgtcg gtcgaagtgg
     4621 tcggcagcat gaaggattcg gaacagacct cgcagatgct gagcaaggcg accgagagcg
     4681 tccaggagct ggtgtcgcgc ttcaagatcg ggcgtggcgc cttcgatttc aacgtggaca
     4741 aggcacgcct gttccgcgac cgggtccagg ataagcttgc ccagttggcc gcgtccggcg
     4801 tcaatgtctg ggatcaggac taccggccgc agccggagac caatccgcag aagtatctgg
     4861 tgagttattg cactgtattc gaacaacaga tgcagccgct gttcgaggag gcattgggca
     4921 gcctgcgtgg cggagtctac gcgctgatcg tcgatgcgcg cggttacggg gcgatccaca
     4981 atcgcaagta ttc
//
LOCUS       DAOSDX010000077         4990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_5983_length_4990_cov_3.427356, whole genome shotgun sequence.
ACCESSION   DAOSDX010000077 DAOSDX010000000
VERSION     DAOSDX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5983_length_4990_cov_3.427356"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2447)
                     /locus_tag="PLN02_02640"
     CDS             complement(<1..2447)
                     /locus_tag="PLN02_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51248.1"
                     /translation="MSDALVKAGKRFGHRWALLAFLALPLIGSWLLHNSQLEKQRLHE
                     EQHIQALSTIYEASLQTYAKATSILFFERIKQEEVLRLMDAASHTRGEAQIPYRARLY
                     RLLAPSYRNLRQLGIRQLHFQTADGNSFLRFHEPQKYGDNLLAIRPSIRKALESRQAV
                     QGFEAGRVKSGFRFVHPLLDGERLLGSVETSIGFRVISDAMHQLQPSREFHFVLAKAT
                     TLPVLFESERWLYGESDIHPDFLVEDPQVVLPDSPPAPSPVLKAINRKLYRMPEVRSG
                     MHDFRAFSISLGIDEQPWVISFLPIKDVSGLSSAYILSYTPAPYIDVLRREFLTQLAL
                     MLVTLGILLFLLLRLIKSRERVVREQRNLVAVTETMGDGLYVLDEYGRVMLINAAALQ
                     LLGFARQDLVGQVGHYLFHRHGDQGCGPLDECPIYRNVKQGKHFFGEEQFSRADGSPL
                     PVEVSSTPLYDGRRIAGSVTAFRDITRRKADELQLRQAMADAEAANQAKSAFLANMSH
                     EIRTPMNGILGLTALTLDSELDATQRQYLELVRQSAESLMLILNDILDFSKMEAGRMV
                     LEVTPFRLRELALGTARVLAARAAEKGLEVLVDIAPEVPDALLGDPGRLRQVLLNLIG
                     NAIKFTEHGEVTLRIALATTGKNPCRLRLAVIDTGIGIDHAKQATIFEAFGQADASVT
                     RRFGGTGLGLTISREIVRLMGGELAVDSTPGAGSNFHFELEFPLAEEGDAGPTPAPEA
                     GIDSLWLLAVRNERLRKLLAAGLRKQRRQVQLCASSESLREQMRQSARLGETARYDVL
                     VAEQDWLPANFAEMPLYR"
     gene            complement(2450..3310)
                     /locus_tag="PLN02_02645"
     CDS             complement(2450..3310)
                     /locus_tag="PLN02_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOI51249.1"
                     /translation="MRFPASRRCAFLFVFFLALLGYRPLLAQTLYFAPLPMEQPEEVI
                     KQFKPLLDYLERRLGTKVEIRYSTSYGEILERFRTGQIDLAYLGPLPYVSLRENYPQA
                     EPLVHFLEKNGQATYSCALIGAGAKLTKPLSGKTFALTQPLSTCGFLAVDGMLRERGE
                     KLENQRFRYLDKHDEVALAVASGDFDFGGMKTAIAQRYAHLGVSVLAETAPLPAFALI
                     ANSRRLSAQRIEELRQALTSLDAKGRDQALLATWGAQLRHGAIVASDADYDVVRRLRR
                     QASIPEKGNF"
     gene            complement(3381..>4990)
                     /locus_tag="PLN02_02650"
     CDS             complement(3381..>4990)
                     /locus_tag="PLN02_02650"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOI51250.1"
                     /translation="TARLGKAFTFHPEDVVAIGHRVVHGGNRFSSSAVITPAVIADIE
                     ELAVFAPLHNPVNVAGIRVALKQFPNVPQVAVFDTAFHQTLAPYAYLYGLPYDLYKQH
                     GIRRYGFHGTSHRYVSLKAAEVLQRPLGELEIVSCHLGIGASLCAIDHGRSVDTTMGM
                     TPTDGLIMPSRSGSIDPAVMIHLMEQHQMSPAQLSTLINCDSGLKGISGISNDIHEIE
                     AAAADGHHRALLAHKAFCYQVRKNIGAYAAAMGGIDVLAFTGDIGETSATVRSLACQG
                     LGYMGIKLDEEKNRHLGKFDSYAVISADDSPVQILVVANDDERLVAWETLRAIERNEL
                     LLEAQAEEAPIPVEISAHHVHLAQADVEALFGPGHQLTPMHELSQPGQYACEEKVHLV
                     GPKGRIANVRVLGPTRKETQVEIAMTEQFKLGVQPPIRQSGDLAGTPGLTLEGPYGSS
                     QIERGVICAQRHIHMSPDDAIRFRVRDNTVVRVRVEGERQLIFGDVVVRVNPAFRLAM
                     HIDTDEGNAGNIQTGMLGYIEEIQSRA"
BASE COUNT          779 a         1471 c         1838 g          902 t
ORIGIN      
        1 cggtagagcg gcatctcggc gaaattggcc ggcagccagt cctgttcggc gaccagcacg
       61 tcgtagcgtg ccgtctcgcc cagtctggcg ctttgccgca tctgctcgcg caacgattcg
      121 ctgctggcgc acagttgcac ctggcggcgc tgcttgcgca ggccggcggc gagcagcttg
      181 cgcaggcgct cgttgcgcac ggcgagcagc cacaggctgt cgatgccggc ttcgggcgcg
      241 ggcgtcggcc cggcgtcgcc ctcctcggcg agggggaatt ccagttcgaa gtggaagttg
      301 ctgccggcgc ccggggtgct gtcgacggcc agctcgccgc ccatcaggcg gacgatttcg
      361 cggctgatgg tgaggcccag tccggtgccg ccgaagcgcc gggtgacgct ggcgtcggcc
      421 tgaccgaaag cttcgaagat ggtcgcctgt ttggcgtggt cgatgccgat gccggtgtcg
      481 atcaccgcca ggcgcaggcg gcacgggttc ttgccggtgg tggcaagggc gatgcgcagc
      541 gtcacctcgc cgtgctcggt gaacttgatc gcgttgccga tcaggttgag cagcacctgg
      601 cgcaagcggc cagggtcgcc gagcagcgcg tcgggaactt cgggggcgat gtcgaccagt
      661 acttccaggc ctttttcggc agcccgcgcg gcgaggactc gggcagtgcc gagcgccagc
      721 tcgcgcaggc ggaacggcgt cacttcgagg accatgcggc cggcttccat cttcgagaag
      781 tcgaggatgt cgttgaggat caacatcagc gattcagccg actggcggac cagttcgagg
      841 tactggcgct gcgtggcgtc gagctcgctg tccagggtca gcgcggtgag gccgaggatg
      901 ccgttcatcg gcgtccggat ctcgtggctc atgttggcga ggaaggcgct cttcgcctgg
      961 ttggcggctt cggcgtcggc cattgcctgg cgcaactgca gttcgtccgc cttgcgccgg
     1021 gtgatgtcgc ggaaagcggt gaccgagccg gcgatgcgcc ggccgtcgta gagcggcgtg
     1081 ctgctgactt cgaccggcaa ggggctgccg tcggcacgcg agaactgttc ctcgccgaag
     1141 aagtgcttgc cctgtttgac gttgcggtag atcgggcatt cgtcgagcgg gccgcaaccc
     1201 tggtcgccat ggcggtgaaa caggtaatgg ccgacctggc cgaccagatc ctggcgcgcg
     1261 aaaccgagca actgcaaggc ggcggcgttg atcagcatga ccctgccgta ttcgtcgagg
     1321 acgtagaggc cgtcgcccat ggtttcggtg acggcgacca agttgcgctg ctcgcgcacg
     1381 acccgctcgc gcgacttgat caggcgcagc agcaggaaga ggagaatgcc cagggtgacc
     1441 agcatgaggg caagctgggt caggaattcg cggcgcagga cgtcgatgta gggggccggc
     1501 gtgtaggaca ggatgtaggc gctggacagg ccggagacat ccttgatcgg caggaaactg
     1561 atcacccagg gttgctcgtc gatgccgagg ctgatcgaga aggcgcgaaa gtcatgcatg
     1621 ccgctacgca cttcgggcat cctgtacagc ttgcggttga tcgccttgag cacgggcgaa
     1681 ggtgctggcg ggctgtccgg caggacgacc tgcgggtcct cgacgaggaa gtcgggatgg
     1741 atgtcggatt cgccgtacag ccagcgctcg gactcgaaca gcacgggcag cgtcgtcgcc
     1801 ttggccagca cgaaatgaaa ctcgcgcgat ggctgtagct gatgcatggc atcgctgatg
     1861 acgcggaagc cgatgctggt ttcaacgctg ccgagcaggc gctcgccgtc gagcagcggg
     1921 tgtacgaagc ggaagccgga cttgacgcgg ccggcctcga aaccctggac ggcttgccgg
     1981 ctttccaggg ccttgcggat cgaggggcgg atggcgagca ggttgtcgcc gtatttctgc
     2041 ggttcgtgga agcgcaggaa actgttgcca tcggcggtct ggaaatggag ctggcggatg
     2101 ccgagctggc gcaggttgcg gtagcttggc gcgagcaggc ggtagagccg ggcgcggtag
     2161 ggaatctgcg cctcgccccg ggtgtgcgag gcggcatcca tcagacgcag cacttcttcc
     2221 tgcttgatcc gctcaaaaaa caggatgctc gtcgccttgg cgtaggtctg caggctggct
     2281 tcgtagatcg tgctcagcgc ctggatgtgt tgttcttcgt gcaggcgttg cttttcgagc
     2341 tgactgttgt gcagcagcca gctgccgatc agcggcagcg ccaggaaggc gagcagcgcc
     2401 cagcggtggc cgaagcgctt gcctgccttg accaatgcgt cggacatgct cagaagttgc
     2461 ctttttccgg gatgctggct tgccgccgca ggcgccggac gacatcgtag tcggcatcgc
     2521 ttgccacaat cgcgccatgg cgcaactgtg ctccccaggt ggcgagcagg gcctggtcgc
     2581 ggcccttggc gtcgaggctg gtcagcgcct ggcgcagttc ctcgatgcgc tgcgcggaca
     2641 ggcggcggct gttggcgatc agcgcgaagg cgggcagcgg cgcggtctcg gccagcacgc
     2701 tgacgcccag atgggcgtag cgctgggcga tcgctgtctt catgccaccg aaatcgaaat
     2761 cgccgctggc gacggcgagg gcgacctcgt catgcttgtc gaggtagcgg aagcgctggt
     2821 tctcgagctt ttcgccgcgt tcgcgcagca tcccgtcgac ggccagaaag ccgcaggtcg
     2881 ataggggttg ggtgagggcg aaggtcttgc cgctcagcgg tttggtcagt ttggcgccgg
     2941 cgccgatcag ggcgcaggaa taggtcgcct ggccgttctt ctccaggaaa tgcaccagcg
     3001 gctcggcctg cggatagttt tcgcgcaggc tgacataggg cagcgggccg aggtaggcga
     3061 ggtcaatctg gccggtgcgg aagcgttcga ggatttcgcc gtaggaggtc gaatagcgga
     3121 tctcgacctt ggtgcccaga cgtctttcca ggtaatcgag cagcggcttg aactgcttga
     3181 tcacttcttc cggctgctcc atcggcaatg gcgcgaagta cagcgtctgc gccagtagcg
     3241 gacggtagcc gagcagggcc aggaaaaaga cgaacaggaa ggcgcagcgg cgggacgcgg
     3301 ggaagcgcat gatcggcgtc gaatcgggac aaagcctcat tctacgccga tcgcccggca
     3361 atccgctggg cggcccgggc ttacgcccgg ctctggattt cctcgatgta gccgagcatg
     3421 ccggtctgga tgttgccggc gttgccttcg tcggtgtcga tgtgcatggc cagccggaaa
     3481 gccgggttaa cgcgcaccac gacgtcgccg aagatcaact ggcgctcgcc ttcgacgcgg
     3541 acgcggacga cggtgttgtc gcgcacccgg aagcggatcg cgtcatccgg cgacatgtgg
     3601 atgtggcgtt gcgcgcagat cacgccgcgt tcgatctggc tgctgccgta ggggccttcg
     3661 agcgtcagtc cgggcgtgcc ggcgaggtcg cccgactggc ggatcggcgg ctggacgccg
     3721 agcttgaact gctcggtcat cgcgatctcg acctgggttt ccttgcgcgt cgggccgagc
     3781 acgcggacgt tggcgatgcg ccccttgggt ccgaccagat gcaccttttc ctcgcaggcg
     3841 tactggccgg gctgcgacag ttcgtgcatc ggtgtcagct ggtggccggg gccgaacagc
     3901 gcctcgacgt cggcttgcgc caggtgcacg tggtgggccg agatttcgac cgggatcggc
     3961 gcctcttcgg cctgcgcttc cagcagcagt tcgttgcgtt cgatggcgcg cagggtttcc
     4021 caggcgacca ggcgttcgtc gtcgttggcg acgacgagga tctgcaccgg cgaatcgtcg
     4081 gcggaaatga ccgcgtagga atcgaacttg ccgagatggc ggttcttctc ctcgtcgagc
     4141 ttgatgccca tgtagccgag gccctggcag gccaggctgc gcaccgtggc gctggtttcg
     4201 ccgatgtcgc cggtgaaggc gaggacgtcg atgccgccca tcgccgccgc ataggcgccg
     4261 atgttcttgc gcacctggta gcagaaggcc ttgtgcgcga gcagcgcgcg gtggtggccg
     4321 tcggcggcgg cggcctcgat ctcgtggatg tcgttggaaa tgccggagat gcccttcaga
     4381 ccgctgtcgc agttgatcag cgtggacaac tgggccggcg acatctggtg ttgttccatc
     4441 aggtggatca tcaccgccgg gtcgatgctg cccgagcggc tgggcatgat caggccgtcg
     4501 gtcggcgtca tgcccatcgt cgtgtcgacc gagcggccgt ggtcgatggc gcacagcgag
     4561 gcgccgatgc cgaggtggca ggagacgatt tccagctcgc cgagcggccg ctgcaggact
     4621 tccgccgcct tcagcgagac gtagcggtgc gaggtgccgt ggaagccgta gcggcggatg
     4681 ccgtgctgct tgtagaggtc gtagggcagg ccgtagaggt aggcgtaggg ggccagcgtc
     4741 tggtggaagg cggtgtcgaa gacggcaacc tgcggcacgt tcgggaactg cttcagtgcg
     4801 acgcggatgc cggcgacgtt gaccgggttg tgcagcggcg cgaagacggc aagttcctcg
     4861 atgtcggcga tcaccgccgg ggtgatcacc gccgagctgg agaagcggtt gccgccgtgg
     4921 acgacgcggt ggccgatggc gacgacgtct tccgggtgga aggtgaaggc cttgccgagc
     4981 cgggcggtgg
//
LOCUS       DAOSDX010000078         4959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6030_length_4959_cov_3.188418, whole genome shotgun sequence.
ACCESSION   DAOSDX010000078 DAOSDX010000000
VERSION     DAOSDX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6030_length_4959_cov_3.188418"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..594)
                     /locus_tag="PLN02_02655"
                     /pseudo
     CDS             complement(49..594)
                     /locus_tag="PLN02_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(862..2019)
                     /locus_tag="PLN02_02660"
                     /pseudo
     CDS             complement(862..2019)
                     /locus_tag="PLN02_02660"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
     gene            complement(2099..2677)
                     /locus_tag="PLN02_02665"
     CDS             complement(2099..2677)
                     /locus_tag="PLN02_02665"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOI51251.1"
                     /translation="MRLYLASRSPRRRELLHQIGVEFDTLIGDVDESPLPGEPAAAYV
                     ERVTRAKAAHGLHLLKERHLVAHPVLAADTTLEFAGEIIGKPADATDAEAILHRLSGQ
                     THRVLTGVAVADAERCAFVLSVSEVSFRVLSDEEIRHYVLSGEPMDKAGAYGIQGRAG
                     LFVAHIAGSYTGIMGLPVCETGELLKQFGWRF"
     gene            complement(2677..3147)
                     /gene="rlmH"
                     /locus_tag="PLN02_02670"
     CDS             complement(2677..3147)
                     /gene="rlmH"
                     /locus_tag="PLN02_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOI51252.1"
                     /translation="MKLAILAVGHRQPAWVDAGCAEYLKRMPREAAASVVEIKPEPRG
                     SKTREQLLAAERGRIEDALPTSCRLVVLDEKGDDLTTLKLAQRLEHWMQDGRDVALLI
                     GGADGIDEALKQRAAEKIRLSSLTLPHGMARLLLCEQLYRAVSVLKNHPYHREG"
     gene            complement(3144..3509)
                     /gene="rsfS"
                     /locus_tag="PLN02_02675"
     CDS             complement(3144..3509)
                     /gene="rsfS"
                     /locus_tag="PLN02_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOI51253.1"
                     /translation="MDIRKLQKIVVSALEDIKGKDIEVINTTKLTSMFDRLVIATGDS
                     NRQVKALARNVQEKVREAGGEIVSVEGEDAGEWVLVDIGDLVVHVMQPAVRSYYNLEE
                     LWQVTPAQRRKLADVAAGE"
     gene            complement(3509..4162)
                     /gene="nadD"
                     /locus_tag="PLN02_02680"
     CDS             complement(3509..4162)
                     /gene="nadD"
                     /locus_tag="PLN02_02680"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOI51254.1"
                     /translation="MSEPLAIFGGTFDPVHFGHLRLAEESLAHLGLDGVRWLPAGNPP
                     HRGVPQVTGEQRLAMVRLATADNPRFAVDAAEVEASAPSYTVHTLERLRGELGAARPL
                     VLLVGADAFAGLAGWHRWREIIELAHVAVSHRPGFPVEAASLPAELAAVFHQRRLTDA
                     SGLRSAPGGGIVTFAMTQLAISATQIRKLLANGLSARYLLPDAVLDYIRAHSLYRNP"
     gene            complement(4155..>4959)
                     /locus_tag="PLN02_02685"
     CDS             complement(4155..>4959)
                     /locus_tag="PLN02_02685"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOI51255.1"
                     /translation="DDLRQLARDEVAALEPQLDAHAALLSPSTGIVDSHALMLSLLVI
                     TYWFIVVFLNLFPMLGALAASLVIPGLSVGLMLAARNIERHQPVSIQTLFGGFRDNTK
                     TLVVLGALYLVATFAVLGLSTVADGGALLKFMLADSRAERALVEDADFSLSALIVVVG
                     MVPVLMAWWFAPVLAAWHRLPLGKSLFFSFVACQMNWRPFVVYGLGLLIVAAMLPGLV
                     LGLLLSLLPGLQGLATTLVTLLMGLVIAPTVFASFYVVYRDIFGISEIV"
BASE COUNT          855 a         1593 c         1742 g          769 t
ORIGIN      
        1 gggcgggagc gcagcaagcg gcgggcagca gcttactgcg aacatgcgct aggaagtcgc
       61 caagcgctgc agcagggtga gttgttccac cgcttcctgc atcgccccca tcccctgcgc
      121 cagttcctcg agacgggcca ggctggcatt gagttcgtca cgttccgtac cggcgttctg
      181 gctcatgttg acgatctggt cggcagcaag cgtgctcttg tcggccattt cctgcgtcgt
      241 gccgacggtt tcggtggcca ccgcgcgtat cttggcgatc gtcgtcaccg cctcgctgat
      301 gctcttggtc gtcgcctgcg cctggtcctt cgccgactgg gcgagcttgc gcacttcgtc
      361 ggcaacgacc gcgaagccgc gcccggcctc gccggcccgc gccgcctcga ttgccgcgtt
      421 gagcgccagc aggttggtct ggtcggcgat cgcgccgatg ccaccggtga tctggctgat
      481 tccctgggag atgcgctcga ggtcgtcgag ttcgctgccc agccgggact gggccgagcg
      541 cgtctgggtg gcagcctcgc tgatcgagcg gatgctgcgc tgggcgatgt ccagcgtgtc
      601 ggcctggcgc agggtggcct gctccagctg gggtagcgcg gcaagcagcg gagctatcgc
      661 ctgctggtaa tcgacgacac gttcggtcac cgcagtctgg atgccgttga gcgccgccca
      721 gcggtgcagg gcgcgctggg cgtagtgccc ggcgtaggcc gcgtaatcga cggggaagcg
      781 cgccatcgcc tcgttgcgct gatcgaagaa gacaagcgcc gacagggtct ggttgatcgg
      841 cacaccgagg atttcgccga aggtcgagaa accggcggcc ggcacgtcgt cgaagaagcg
      901 cgcctcgctc agccgcccgc tgttgccgac gcggcgcagc acgcaatcgt tgagcagcac
      961 ggcgctcggc cggccatggc ggctgacgaa ctgctgccag tcgttgcgcg tggcggcgat
     1021 gaaatcggtg gccttgagca ggtgcagccg gtcgccgaat tcgaggtcgc agaagaaggc
     1081 gatgcggtcg ttacggattt ccgcgaccga gcggatgaaa tactcgtcct cgaccttgac
     1141 cgcgaaggtc ttgccttcca gctgccgggc cagatcgccg cggtcgcaac gcagatgcgc
     1201 ggcgagcacg tcgaggatgg gctgcggccg gccgtcgctg ccgaacagcg aggtgacggt
     1261 acgggcgacc gggtcggcct cggcgaccag ccagctttgc tgggtcggtt cgaagttctg
     1321 gctcttgaac ggggcaaagg atttgccctc cgccatctgg cagaagacca gcaccgcctt
     1381 gccctgcagc accgagccgc cggcgccgat gtaggtgccg ctgaaattga gccggccgcc
     1441 ggcggagcca ccgatcgcca gacaggggaa acgcccgctc tggtaccagg cctgcatcag
     1501 gaaaccttca gacgccgaca gcccgtcaca gaagacgagg gcgaagctgc gctcgccgga
     1561 gagcgggatg cccggccgga tttgctccag ttcgcggcgg atggcggcga cgcgctggct
     1621 ggcggtggcc gtgtccttga tgtggaggtc gacgacatgc acctcgtggc gggcgatcag
     1681 gccctgcggc agccacaacc agctgccctg cgtgccttgc atctcgcagt agggagaagc
     1741 gccagcctga ccgcacaggg cgccggtcga cgacaggctg agcatcgagc ggccgctacc
     1801 ggacaagcgc tgccaggccg tactgacccg cgaaaaatcg gtttccggcg gcacgaagga
     1861 aagcagcagg ccggcttcgc cggcgacgcc gagctggctg aagtcggtcg gcggaacgtt
     1921 acagtcgaag cgcccactct gcggctggcc cgcctgaggc gccccggatg tcggccgtgc
     1981 taccgacgta tcgccggaaa acagcgaaaa caggcccatg atgtctctcc tcggttgtcg
     2041 ttatgaggag caatgtagcg agccgtggcg acgccggcaa gctaggcccg caagcggctc
     2101 agaaacgcca gccgaactgc ttgagcagct ccccggtttc gcacaccggc agacccatga
     2161 tgccggtata gctgccggcg atatgggcga cgaacagccc ggcccggccc tggatgccat
     2221 aggcgccggc cttgtccatc ggctcgccgg agaggacgta gtggcggatc tcctcatcgg
     2281 agaggacgcg gaaggagact tcgctgaccg agagaacgaa ggcgcagcgc tcggcgtcgg
     2341 cgacggcgac gccagtcagg acgcggtgcg tctgcccgga caaacggtgc aggatggcct
     2401 cggcgtcggt ggcatcggcc ggcttgccga tgatctcgcc ggcgaattcg agcgtggtgt
     2461 cggcggccag caccgggtga gcgaccaggt gacgttcctt gagcaggtgc aggccatgcg
     2521 ccgccttcgc ccgcgtcacg cgttcgacgt aggcggcagc cggctcgccg ggcaaggggc
     2581 tttcatcgac atcgccgatc agggtatcga actcgacgcc gatctggtgc agcagttcgc
     2641 gccggcgcgg gctacgcgag gccaggtaga gacgcatcag ccttcccggt ggtaggggtg
     2701 gttcttcagc acgctgaccg cgcggtacaa ctgctcgcac agcagcaggc gcgccatgcc
     2761 gtgcggcagg gtcaggctgg acaggcggat cttctcggcg gcgcgctgct tgagcgcctc
     2821 gtcgatgccg tcagcgccgc cgatcagcaa cgcgacgtcg cgcccgtcct gcatccagtg
     2881 ttcgagccgc tgcgccagct tcagcgtggt caggtcgtcg cccttctcgt cgaggacgac
     2941 caggcggcag gaggtcggca gggcatcctc gatccgcccg cgttcggcgg cgagcagttg
     3001 ttcgcgggtt ttcgagccgc gcggttcggg cttgatctcg acgacactgg ccgccgcctc
     3061 acgcggcatg cgcttgaggt attcggcgca accggcatcg acccaggcgg gttgccggtg
     3121 gccgacggcg aggatggcca gtttcattcg ccggcggcga cgtccgccag cttgcgccgc
     3181 tgggccgggg tcacttgcca gagttcctcg aggttgtagt agctgcgtac cgcgggctgc
     3241 atgacgtgga cgaccaggtc gccgatatcg accagcaccc actcaccggc gtcctcgcct
     3301 tcgaccgaga cgatttcgcc gccggcctcg cgcaccttct cctggacgtt gcgggccagc
     3361 gccttgacct ggcggttgga atcgccggtg gcaatgacca ggcgatcgaa catcgaggtc
     3421 agcttggtgg tgttgatcac ttcgatgtcc ttgcccttga tgtcttcaag ggcggaaacg
     3481 acaatttttt gcagcttgcg tatgtccatc agggatttct gtaaagggaa tgggcgcgaa
     3541 tatagtcgag aacggcgtcc ggcagcaaat agcgcgccga caagccgttg gccagcagct
     3601 tgcggatctg cgtcgccgag atggcgagct gggtcatcgc gaaggtgacg atgccgccgc
     3661 ccggcgcgct acgcaggcca ctcgcatcgg tcagccggcg ctggtgaaag acggcggcca
     3721 gctccgccgg caggctggcg gcttcgaccg gaaagccggg acggtgcgag accgcgacat
     3781 gggcgagttc aatgatttcg cgccagcggt gccagcccgc caggccggcg aaggcatcgg
     3841 cgccgacgag caggaccagc ggccgggcgg cacccagttc gccacgtagg cgttcgagcg
     3901 tgtgtaccgt gtagcttggt gcgctggctt cgacttcggc ggcatcgacc gcgaagcggg
     3961 ggttgtcggc ggtggccagg cggaccatcg ccagccgctg ctcgccggtc acctgcggca
     4021 ccccgcggtg cggcggattg ccggccggca gccagcgcac gccgtccagc ccgagatggg
     4081 cgagcgactc ctcggccagc cgcagatgtc cgaaatgcac cgggtcgaaa gtcccgccga
     4141 aaatcgccag cggctcagac aatttcggag atgccgaaga tgtcgcgata gaccacgtaa
     4201 aagctggcga acacggtcgg cgcgatgacc agacccatca gcagggtgac cagcgtcgtc
     4261 gccagcccct gcagccccgg caacaggctg agcagcaagc ccagcaccag gccgggcaac
     4321 atggcggcga cgatcagcag gcccagcccg tagacgacga aggggcgcca gttcatctgg
     4381 caggcgacga aactgaagaa cagcgacttg ccgagcggca gccggtgcca ggcggcgagc
     4441 accggcgcaa accaccaggc catcagcacc ggcaccatgc caacgacgac gatcagcgcc
     4501 gacaagctga agtcggcatc ctcgaccagg gcgcgttcag cgcggctgtc ggcgagcatg
     4561 aacttgagca gggcgccacc gtcggcgacg gtcgacaggc cgagcacggc gaaagttgcc
     4621 accaggtaga gggcgccgag cacgaccaga gtcttggtat tgtcgcggaa accgccgaac
     4681 agggtctgga tggaaaccgg ctggtgacgt tcgatattgc gcgccgccag catcagaccg
     4741 accgacaagc caggaatgac cagcgacgcg gccagcgcgc cgagcatcgg aaacaggttg
     4801 agaaagacga cgatgaacca gtaggtgatg accagcagcg acagcatcag cgcgtggctg
     4861 tccacgatgc cggtggaggg cgacagcagg gcggcatggg cgtccagttg cggttccagc
     4921 gcagcgactt cgtcgcgggc gagctggcgc aggtcatcc
//
LOCUS       DAOSDX010000079         4881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6141_length_4881_cov_2.973891, whole genome shotgun sequence.
ACCESSION   DAOSDX010000079 DAOSDX010000000
VERSION     DAOSDX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6141_length_4881_cov_2.973891"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLN02_02690"
     CDS             <1..415
                     /locus_tag="PLN02_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51256.1"
                     /translation="LASTLAAAGATRLVALPIRTPSHTARLLSATNAFAEHLLALPDA
                     RLSFPVFSAVDATAARRVAPALRALARQLAEPLDWAACLQAIREMQPDAVLEIGPGNA
                     LARLFGEMAPEIPVRASDDFRSSDGIVAWLASCAG"
     gene            426..1232
                     /locus_tag="PLN02_02695"
     CDS             426..1232
                     /locus_tag="PLN02_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOI51257.1"
                     /translation="MSLMLLAAYLATGAVAGFFAGLLGVGGGVVVVPMLALLFAGQGF
                     PENEILHLALGTSMATILFTAASSLRAHHAHRAVLWPTVRRLTPGILLGTLCGAYLAT
                     FVSSRLLALIFAAFMLFVASQMISQRRPRADRSLPGALGLSLAGGVIGAFSSLVAIGG
                     GALTVPFLIWCGVRPQQAIGTSAAVGLPIAIGGTCGYVWHGWGHAGLPPGSLGFVYLP
                     ALAVILVASVLVAPLGARLTHQLPVRLLRGIFAGLLLLLSAKMLWNLFAP"
     gene            complement(1243..2019)
                     /locus_tag="PLN02_02700"
     CDS             complement(1243..2019)
                     /locus_tag="PLN02_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOI51258.1"
                     /translation="MSSIDLKIDGEIATLTLNNPAKLNAIDLAMWRALSHHMATLAAD
                     SSVRCIVLRGAGEAAFAAGGDLEEFVSARTTLDQALLYHGEVAAALNAIADSPHPTVA
                     LIQGACIGGGLEIAGACDLRIAGEGAKFGAPINKLGFSMYPGEMEGLLRLAGAAVVKE
                     ILLEGRILGAREAYEKGLLTRVVPDVQVADEAYASARRICQGAPLVAGWHKQWIRRLQ
                     DGRPLTDAEKAASFAFLDTEDYREGLAAFLEKRKPQFKAR"
     gene            2136..2456
                     /locus_tag="PLN02_02705"
     CDS             2136..2456
                     /locus_tag="PLN02_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOI51259.1"
                     /translation="MSSVMYERMRGNPKFQELVAKRGRFAWTLAAIVLTMFYGFVMIV
                     AFAPASLGQPIAEGSRWTVGVVAELFMFIFFWVLTAVYVRRANTEFDALSQEVVRDAW
                     KENK"
     gene            2456..4120
                     /locus_tag="PLN02_02710"
     CDS             2456..4120
                     /locus_tag="PLN02_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOI51260.1"
                     /translation="MRKIIPALGLLGLSTLAFAAGDALGPTEQQATNWHAIIMFCLFV
                     AMTMGITYWAASRTKSTSDFYTAGGGITGFQNGLAIAGDYMSAATLLGLTAMVYSKGY
                     DGYIYMLCFFAGWPIILFLMAERLRNLGKFTFSDITAYRLDQGKVRTMAAVSSLVVVC
                     FYLIAQMVGAGQLIKLLFGLEYNVAIFAVGILMMVYVTFGGMVATTWVQIIKACMLLA
                     GGTLVMVLAMSQFGFSFGALVDQAMSVHTLGEKLMHPGSLLADPVTAISLGLGLMFGT
                     AGLPHILMRFFTVTDAKEARKSVLYASGFVAYFFNVIFLMGLAGIIIVGQNPEFFEGG
                     EIGKKLIGGGNMVAMHLAKAVGGDMLLGFLAAVAFATILAVVSGLALAGASAISHDIY
                     ARVIKKGTASEAAEIRVSKMATIALGFVAIILGIAFEKQNIAFMVGLAFGVAAAANFP
                     VLILSMYWKGLTTRGALWGGYGGLISAVLFVLFSKSVWVDVLGNAAPLFPYTQPALFA
                     MPVAFLLAFIFSKSDSSVRAKSEIEAFDDQYVRAQTGYGAAGAAKH"
     gene            4315..4734
                     /locus_tag="PLN02_02715"
     CDS             4315..4734
                     /locus_tag="PLN02_02715"
                     /inference="COORDINATES: protein motif:HMM:NF021457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HOI51261.1"
                     /translation="MENTLKEKLDGLDKLVGGLMGGLAVQLHPIAGPVPVVQVSIETR
                     EELPIFITCSDSQILCLCYLWSDSEVLPERRTELLETLLDLNPSVPLSSFGRIGERYV
                     LSGALARDARGEDIAQDIAALSDNALDALDALSEFLH"
BASE COUNT          780 a         1616 c         1498 g          987 t
ORIGIN      
        1 gctcgccagc acgctggcgg cggccggtgc gacgcgcctg gtggcactac cgattcgcac
       61 cccttcgcat acggcgcggc tcttgtctgc gacgaatgct ttcgcggaac atctgctggc
      121 attgcccgat gcccgtttgt ctttccccgt gttcagtgct gtcgatgcga cggcagcacg
      181 ccgggttgca ccagcgcttc gcgcactggc ccgccagttg gccgagccgc tcgattgggc
      241 agcctgcctg caggcaattc gcgagatgca gccggatgcg gttcttgaga tcgggccggg
      301 caatgccctg gcgcgactgt tcggggaaat ggcgccggag attccggtac gggccagcga
      361 cgattttcgg agcagcgacg gtatcgtcgc ctggctggcg tcatgcgccg gttgaggctg
      421 gcttgatgtc gctgatgctg cttgccgcct atctggcgac tggtgcggtc gccggctttt
      481 tcgccggcct gctcggcgtc ggcggcggcg tcgtggtggt gccgatgctg gccttgctgt
      541 tcgccggaca aggcttcccg gaaaacgaaa tactgcatct ggcgctcggc acctcaatgg
      601 cgaccatcct gttcaccgcc gcttccagtc tgcgcgccca ccatgcccac cgggccgtgc
      661 tctggccaac ggtacgtcga ctgaccccgg gcatcctgct cggtaccttg tgcggcgcct
      721 atctcgcgac tttcgtctcg tcgcgcctcc tggccttgat cttcgctgcc ttcatgctct
      781 tcgtcgccag ccagatgatc agccaacgac gtccgcgagc cgaccgcagc ctgcccggag
      841 cgctcggcct gagcctggcg ggcggcgtga tcggcgcttt ctcgagtctg gtggcgatcg
      901 gcggtggcgc cctgacggta cctttcctga tctggtgcgg cgttcgcccc cagcaggcga
      961 tcggcacctc ggccgcggtc ggcctgccga tcgccatcgg cggcacctgc ggctatgtct
     1021 ggcatggctg ggggcatgcc gggctgccgc cgggcagtct cggcttcgtc tatctgccgg
     1081 cgctggccgt catcctcgtc gccagcgtgc tggtcgcgcc gctcggcgcg cggctcacgc
     1141 accaactacc ggtgcgcctg ctgcgcggca tcttcgccgg gctgttgctg ctgctttcgg
     1201 cgaagatgct gtggaacctg ttcgccccct gagtccggcg cttcaacgcg ccttgaactg
     1261 cggcttgcgc ttttccagga aggcggccag cccctcgcgg taatcctcgg tgtcgagaaa
     1321 ggcgaacgag gcggcctttt ccgcgtcggt cagcggccgg ccgtcctgca gacgacggat
     1381 ccactgcttg tgccagccgg cgacgagcgg cgccccctgg cagatgcggc gggcgctggc
     1441 ataggcttcg tcggcgacct ggacgtcggg cacgacgcgc gtcagcaggc ctttttcgta
     1501 agcctcgcgg gcgccgagga tgcgcccttc gagcaggatt tccttgacca cggcagcacc
     1561 ggcgagacgc agcaggcctt ccatctcccc cggatacatc gaaaagccga gcttgttgat
     1621 cggcgcgccg aacttcgcac cttcgccggc aatacgcagg tcgcaggcgc cggcgatctc
     1681 cagcccgccg ccgatgcagg cgccctggat cagtgccacc gtcgggtgcg gcgaatcggc
     1741 gatggcgttg agcgcggcgg caacttcgcc gtgatagagc agcgcctgat cgagcgtggt
     1801 ccgggcactg acgaattcct cgagatcgcc gccggcggcg aaagcggcct cgccagcacc
     1861 gcgcaggacg atgcaacgga cgctggaatc ggcggccagc gtcgccatgt ggtggctcaa
     1921 ggcgcgccac atcgccaggt cgatggcatt cagcttggcg ggattgttca gggtcagggt
     1981 ggcgatttca ccgtcaattt tcaggtcaat gctgctcatg ttccatgtcc aaaattacgc
     2041 atgcgtaaac ttacatacac tgtaaggtta tcgggtctta tataagatac aatgcgcacg
     2101 ccgcttagcg gtaattatta ataaggagaa caactatgag cagcgtgatg tacgaaagga
     2161 tgcggggaaa tcccaaattc caggagctgg tcgcaaagag gggacgtttc gcctggaccc
     2221 tggccgcgat cgttttgacc atgttctacg gcttcgtgat gatcgtggcg ttcgcgcccg
     2281 cgtcgctcgg ccagccgatt gccgaaggtt cgcgatggac cgttggcgtc gtcgccgaac
     2341 ttttcatgtt cattttcttc tgggtgctga ccgcggtcta tgtccgccgc gccaataccg
     2401 aattcgacgc cctttcccag gaagtggtgc gtgacgcctg gaaggagaac aaataatgcg
     2461 caagatcatt cccgcgctcg gactgctcgg cctgagcact ctggcctttg ccgccggcga
     2521 cgccctcggc ccgacggaac agcaagccac caactggcac gccatcatca tgttctgcct
     2581 gttcgtcgcg atgacgatgg gcatcaccta ctgggcggcc agccgcacca agtcgacgtc
     2641 cgacttctac accgccggtg gtggcatcac cggtttccag aacggcctgg cgatcgccgg
     2701 tgactacatg tccgccgcca ccctgctcgg tctgacagcg atggtgtaca gcaagggcta
     2761 cgacggctac atctacatgc tgtgcttctt cgccggctgg ccgatcatcc tcttcctgat
     2821 ggccgaacgt ctgcgcaacc tgggcaagtt caccttctcc gacatcaccg cctaccgcct
     2881 ggatcaaggc aaggtgcgca ccatggccgc ggtttcctcg ctggtcgtcg tctgtttcta
     2941 cctgatcgcc cagatggtcg gtgccggcca gctgatcaag ctgcttttcg gcctcgaata
     3001 caacgtcgcg atcttcgccg tcggcatcct gatgatggtc tatgtcacct tcggcggcat
     3061 ggttgccacc acctgggtgc agatcatcaa ggcctgcatg ctgctggccg gtggcacgct
     3121 ggtcatggtg ctggcgatga gccagttcgg cttctcgttc ggcgccctgg tcgatcaggc
     3181 gatgtcggtc cacaccctgg gtgaaaagct gatgcacccg ggcagcctgc tcgccgaccc
     3241 ggtcaccgcg atctcgctcg gcctcggcct gatgttcggt accgccggcc tgccgcacat
     3301 cctgatgcgc ttcttcaccg tcaccgacgc caaggaagcc cgcaagtcgg tgctctatgc
     3361 ttccggcttc gtcgcctact tcttcaacgt catcttcctg atgggcctcg ccggcatcat
     3421 catcgtcggc cagaaccccg agttcttcga aggcggcgag attggcaaga agctgatcgg
     3481 cggcggcaac atggttgcca tgcacctggc caaggcggtc ggcggcgaca tgctgctcgg
     3541 cttcctcgcc gcggttgcct tcgccaccat cctggcggtg gtttccggtc tcgccctggc
     3601 cggtgcctcg gcgatctcgc acgacatcta cgctcgcgtg atcaagaagg gtacggcttc
     3661 cgaagctgcg gaaatccgcg tctccaagat ggccaccatc gccctcggct tcgtcgccat
     3721 catcctcggc atcgccttcg aaaagcagaa catcgccttc atggtcggcc tcgccttcgg
     3781 cgtcgctgcc gccgccaact tcccggtcct gatcctctcc atgtactgga agggcctgac
     3841 cacgcgcggc gcgctgtggg gcggttacgg cggcctgatc tcggcggtgc tgttcgtgct
     3901 cttctcgaag tcggtgtggg ttgacgtgct gggcaacgcc gccccgctct tcccctacac
     3961 ccagccggcg ctgttcgcga tgccggtcgc cttcctgctc gccttcatct tctcgaagag
     4021 cgactcgagc gtccgtgcca agagcgaaat cgaagctttc gacgaccagt acgtccgtgc
     4081 ccagaccggc tacggcgctg ctggcgcagc caagcactaa gcccgctgcg gctttcaccc
     4141 gaaaccccga ccggtccgcc ggtcggggtt ttttattgcc tgtgcctcac acctagtcat
     4201 gaaccaccgg caaatgtcat attatagcga catgcgtcgt ctgacataaa atgtcttgac
     4261 attttatgtc tccacttcta gtctggatgc cacacgaacc ataacgagga caacatggaa
     4321 aacacactga aggaaaagct ggatgggctg gacaagctgg tcggcggcct gatgggcggc
     4381 ctcgcggtac agttgcatcc gattgccgga ccggtgccgg tggtccaggt cagcatcgag
     4441 acgcgcgaag aactgccgat cttcatcacc tgttcggatt cgcagatcct gtgcctgtgc
     4501 tacctgtgga gcgacagcga agtgctgccg gaacgccgca ccgaactgct cgaaacgctg
     4561 ctcgacctca acccgtcggt gccgctttcg tctttcggcc gcatcggcga gcgctacgtg
     4621 ctgtccggcg ccctggcgcg cgacgcgcgc ggcgaggaca tcgcccagga catcgccgcc
     4681 ctgagcgaca acgcgctcga tgcgctggac gccctgtccg aatttctgca ctgaaggaga
     4741 cgaacatgtc cgtgatcaag aaagtggtga ccctgttgcg cggcagcgcc cgcgaactgg
     4801 ccgagaacgt cgtcgatgcc aacgccacgc gcatctacga gcaggaaatc atcgacgcca
     4861 agcagagcat cctcgaagcg c
//
LOCUS       DAOSDX010000080         4878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6147_length_4878_cov_3.229525, whole genome shotgun sequence.
ACCESSION   DAOSDX010000080 DAOSDX010000000
VERSION     DAOSDX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_4878_cov_3.229525"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..2707
                     /gene="ggpS"
                     /locus_tag="PLN02_02720"
     CDS             470..2707
                     /gene="ggpS"
                     /locus_tag="PLN02_02720"
                     /EC_number="2.4.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033828 - glucosylglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerol-phosphate synthase"
                     /protein_id="HOI51262.1"
                     /translation="MLLATDLDGTFLGGSAEHRQTLYQLVSGSQDIQLVFVTGRGLEA
                     VMPLLSDPLVPTPDYILCDVGGTVVEGVSLQPVQPLQAAIDMRWPGERMVADAMAAFP
                     ELERQEVPQQRRCSYFCHQDAVTPALIEAARALGCEVMYSASRYLDLLPVGVNKGSTL
                     RALVDQLGVAHHEVLVAGDTLNDLAMYEAGFQGVCVGDSEPALLAATGNLTNALHASD
                     PGCGGILQAIVHFSLLRPGYLDAATGNAKRGPAELVMVYHRLPYEEYRINGRIVQRRP
                     KSPNGIIPTLLSFFATGQRGSWVAWSIHDEARDPPFEVHTTVDARAYPGLLAARVALS
                     KEEVDVFYKRFSKEAFWPTLHTFWERAVFREADWQVFLAVNRKFAERTAAEAAEGATV
                     WLHDYNLWMVPAALRELRPDLKIAFFHHTYFPSADAFNVLPWRREIVGSLLQCDYIGF
                     HIPRQAENFVDVAAGVASLRVLERRACAPRYLSYGCAVGLETMTTRIEVHGRQIGLGA
                     HPVGLDLGRIGRSLQHPKTPGRRTAIRDEFAGKHLVLSVERLDYTKGTLEKIRAFGDL
                     LERHPQMRAKVSLVVVCVPAAKEMTVYDELQVQIEQAVGRVNGQYAEVGWSPIRFFFR
                     PLPFEEVVAWYAEADVMWITPLRDGLNLVCKEYVATQGQVDGRGVLVLSEFAGAAAEL
                     HGALLTNPHDPADLRERLRQALEMDPVERESRLRGLNEIVRHNDIERWGREFLEAVAA
                     AGVPR"
     gene            2774..3874
                     /locus_tag="PLN02_02725"
     CDS             2774..3874
                     /locus_tag="PLN02_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOI51263.1"
                     /translation="MQQLDSWLSRLTGGDPTLGLALQVFLVVSLVAVGNFLLRRLLDR
                     LAARTAHTANPWDEALVNAARRPLPLLAWIVGLSFAARIVQGEADAALFDAIDPLRTI
                     GVIASLTWFLVRLIGNMQAAILQRQLDRGESVDRTTVDAIGKLLRVSVLITAVLVGLQ
                     SLGFSISGVLAFGGIGGIAVGFAAKDLLANFFGGLMVYLDRPFTVGDWIRSPDKEIEG
                     TVEDIGWRLTRIRTFDKRPLYVPNSIFTQITVENPSRMSHRRIMERIGVRYDDMAAVE
                     GIVAEIREMLRVHPEIEPSQTTIVNLDAFGASSVDILIYTFTRTTVWVDYHAIKQDVL
                     LRIAAIVAAHGAEIAFPTRTLHLVPEASPAAV"
     gene            complement(3883..>4878)
                     /locus_tag="PLN02_02730"
     CDS             complement(3883..>4878)
                     /locus_tag="PLN02_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI51264.1"
                     /translation="VACAALLHELLASAWVWGLLRLVTGMAMVGLYTTIESWLNSHAA
                     PPQRSRIFATYMAVNLGSLALAQQLLHWGDPGGHVLFALAALSFCAAVMPVAATRLAQ
                     PVVEEVHGIGLATLYRRAPVACAAGLLSGFAQGAFWGLAPVWADTSQLGHAGVAWFMS
                     IAIVGGAAFQWPIGVLTNRLDRGHVISVIALIAALCAGGLLLAAEHGLRLVLAAAFAY
                     GGFAFAMYPLAVARLMDRLEKPEIIAGSGSLLLLHGIGASLGPLLAGTLMHHFGPSAL
                     PVWFIAIQCLLAVTAWWLSVRAPADIAHQTPLTPMERTTPGVIEMLEPESTAPTR"
BASE COUNT          764 a         1538 c         1667 g          909 t
ORIGIN      
        1 ctgggggtgc tggtggaagt attggggctg acgctgctca ccatgggcat attcgtgcta
       61 tcgcaggctt ggccacgcct atgagcatcc aggggcgttt tgcccaatag cgcccagcag
      121 ttttttcggg gcgtgattgg acgaggatgg cgtcgatgcg cagggcccgg cgagccgttg
      181 gagcgggggg ggggcgaggg aatccagtgg aggtgtgaac ctggggggca ttctcccggg
      241 cttgctgtcc tgatatgttg gtttgcaaac taatttattg gctttgtttt gtgatgaatt
      301 aaaaaatata ttttgaatca atcatctata ttgctacaat attttgtgtg gctgaaatct
      361 gcattgattt tcaatgcttt tttgtgtcgt atcgctaggg gtgcggcgtt ttttggtgat
      421 tgatgtcctg tggtctggcc gctccggggg ggcgtccggg agtgacttga tgttattggc
      481 aaccgatctc gacggaacct ttctcggggg gtcggcggag caccgccaaa ccctgtatca
      541 actggtttcc ggcagtcagg acatccagct ggtattcgtc accgggcgcg ggctggaggc
      601 ggtgatgccg ctgttgtccg atccgttggt gccgacgccc gattacatcc tctgcgatgt
      661 cggcggcacc gttgtcgagg gcgtctcctt gcagccggtg caacccctgc aggccgccat
      721 cgacatgcgc tggccgggcg agcgcatggt cgccgatgcg atggcggctt ttcccgaact
      781 ggaacgccag gaagtgccgc agcagcggcg ctgttcctat ttctgtcatc aggacgcggt
      841 gacgccggcg ctgatcgagg cggcgcgcgc actcggttgc gaggtcatgt actcggcgag
      901 ccgctatctc gatctgttgc cggtcggcgt gaacaagggc agcaccctgc gggcgctggt
      961 cgaccagctc ggcgttgccc atcatgaagt gctggtggcc ggcgacacgc tcaacgacct
     1021 ggcgatgtac gaagccggtt ttcagggggt ctgtgtcggc gattccgagc cggccctgct
     1081 ggcggccacc ggtaatctga ccaacgcctt gcacgcgagc gatcccggtt gcggcggcat
     1141 cctgcaggcg atcgtccatt tttcgctgct gcggccgggc tatctcgacg cggcgacggg
     1201 caacgccaag cgcggcccgg ccgaactggt catggtctat caccggctgc cctacgaaga
     1261 gtaccggatc aacgggcgta tcgtgcagcg acggccgaaa tcgccgaacg gcatcatccc
     1321 gaccctgctt tcctttttcg ctaccgggca acgcggatcc tgggtcgcct ggagcatcca
     1381 tgacgaggcg cgcgacccgc ccttcgaggt gcataccacg gtcgatgccc gggcctatcc
     1441 cggtctgctc gccgcccgcg ttgccttgtc caaggaggaa gtcgacgtct tctacaagcg
     1501 tttctccaag gaggccttct ggccgaccct gcataccttc tgggagcggg cggtgttccg
     1561 cgaggcggac tggcaggtgt ttctcgccgt caaccgcaag ttcgccgaac ggaccgccgc
     1621 cgaggccgcc gagggggcga ccgtctggct gcacgattac aacctgtgga tggtgccggc
     1681 ggcactgcgc gagttgcgcc ccgacctgaa gattgccttc ttccatcaca cctatttccc
     1741 gtcggccgat gccttcaacg tcctgccctg gcgccgcgaa atcgtcggca gcctgctgca
     1801 gtgcgattac atcggcttcc acattccacg gcaggcggag aatttcgtcg atgtcgcggc
     1861 cggcgtggcg tcgctgcgtg tactggaaag gcgcgcctgc gcgccgcgtt acctgagcta
     1921 cggttgcgcc gtcggcctgg aaacgatgac tacccggatc gaggtccatg ggcggcagat
     1981 cggcctcggc gcgcatccgg tcggcctcga tcttgggcgc atcgggcgtt ccttgcagca
     2041 tccgaaaacc cccgggcggc ggacggcgat ccgtgatgag tttgccggca agcacctggt
     2101 gctgtcggtc gaacgactcg attacaccaa gggcacgctg gaaaagatcc gcgccttcgg
     2161 cgatctgctc gaacgccatc cgcagatgcg cgccaaggtg tcgctggtcg ttgtctgcgt
     2221 gccggcggcc aaggaaatga ccgtgtacga cgaactgcag gtgcagatcg agcaggcggt
     2281 gggccgcgtt aacggtcagt atgccgaggt cggctggtcg ccgatccgct tcttcttccg
     2341 gccgttgccg ttcgaggagg ttgttgcctg gtacgccgag gccgacgtga tgtggatcac
     2401 ccccctgcgc gacgggctca acctggtgtg caaggagtac gtggcgaccc aggggcaggt
     2461 cgacgggcgc ggcgtcctgg tgctctccga gttcgccggc gccgccgccg aactgcacgg
     2521 tgcgctgctg accaacccgc acgatccggc cgacctgcgc gagcgtctgc gccaggcgct
     2581 ggagatggat ccggtcgagc gcgaatcgcg cctgcgcggc ctcaacgaga tcgtccggca
     2641 caacgacatc gagcgctggg gccgggagtt cctcgaggcg gtcgccgccg ccggcgtccc
     2701 gcgctgaacg cgcaagtcct gtcatctggc gatggcagac tgttttttcc cccggtcatg
     2761 agattctgtg gacatgcaac aacttgattc ctggctgagc cgcctgaccg gcggtgatcc
     2821 caccctgggc ctggcgctgc aggtttttct ggtcgtttcc ctggtcgccg tcggcaattt
     2881 cctgttgcgc cggctgcttg accggctcgc cgcccgcacc gcgcacaccg ccaatccctg
     2941 ggacgaggca ctggtcaacg cggcacgccg ccctttgccc ctgctggcct ggatcgtcgg
     3001 tctcagcttt gccgcgcgca tcgttcaggg cgaggcggac gccgccttgt tcgatgccat
     3061 cgatccgctg cgcacgatcg gcgtcatcgc cagcctgacc tggttcctgg tcaggctgat
     3121 cggcaacatg caggccgcca tcctgcaacg ccagctcgac cgcggcgaaa gcgtcgaccg
     3181 gacgacggtg gacgcgatcg gcaagttgct gcgggtgtcg gtgctgatca ccgccgtgct
     3241 ggtcggcctg cagagcctgg gcttctcgat ctccggcgtc ctcgcctttg gcgggatcgg
     3301 cggcatcgcc gtcggcttcg cggcgaagga cctgctggcc aatttcttcg gcggcctgat
     3361 ggtctatcta gaccggccat ttaccgtcgg tgactggatc cgttcgccgg acaaggagat
     3421 cgaaggcacg gtcgaggaca tcggctggcg cctgacgcgc atccgcacct tcgacaagcg
     3481 gccgctctac gtgccgaatt cgatcttcac ccagatcacc gtcgagaacc cgtcgcgcat
     3541 gagccatcgc cgcatcatgg agcgcatcgg cgtccgttac gacgacatgg cggcggtcga
     3601 aggcatcgtc gccgagatcc gtgaaatgtt gcgggttcat ccggaaatcg agccgtcgca
     3661 gacgacgatc gtcaatctcg acgccttcgg cgcctcgtcg gtcgatatcc tgatctatac
     3721 cttcacgcgg accacggtct gggtcgatta ccacgcgatc aagcaggatg tcctgctccg
     3781 gatcgccgcc atcgttgccg cgcatggcgc cgaaatcgcc ttcccgacac ggaccctgca
     3841 cctggttccg gaggcctcgc cggcggccgt ctgatcgggg gctcagcgcg ttggcgcggt
     3901 gctttccggt tcgagcatct cgatgactcc cggcgtcgtc ctttccatcg gcgtcagcgg
     3961 cgtctggtgg gcaatatcgg ccggggcccg cactgacagc caccaggcgg tgacggcgag
     4021 caggcactgg atggcaatga accagacggg gagggcggac gggccgaaat ggtgcatcag
     4081 ggtaccggcg agcagcgggc cgaggctggc gccgatgccg tgcagcagca gtaggctgcc
     4141 gctgccggcg atgatctcgg gtttttccag gcggtccatc aggcgggcga cggccagcgg
     4201 gtacatggcg aaggcgaagc ccccgtaggc gaaagccgcc gccagcacga ggcgcaggcc
     4261 atgctcggcg gcgagcagca gaccgccggc gcagagggcg gcgatcagcg cgatgacgct
     4321 gatcacgtgg ccgcggtcga gacggttggt gaggacgccg atcggccact ggaaggcggc
     4381 gccgccgacg atggcgatgc tcatgaacca ggcgacgcca gcgtggccga gctggctggt
     4441 gtcggcccag accggcgcca ggccccagaa ggccccctgg gcgaagccgg agagcaggcc
     4501 ggcggcgcag gcgaccggcg cccgccggta gagggtggcg aggccgatgc cgtgcacttc
     4561 ctcgaccacc ggctgggcca ggcgggtcgc cgccacaggc atcacggcgg cgcagaagga
     4621 cagggcagcg agcgcgaaca gcacgtgccc gccggggtcg ccccagtgca gcagttgctg
     4681 cgccagcgcc agcgagccga ggttgaccgc catgtaggtg gcgaagatgc ggctgcgctg
     4741 gggcggtgcg gcgtggctgt tgagccagct ttcgatggtc gtgtacaggc cgaccatggc
     4801 catcccggtg accaggcgca gcagtcccca gacccaggcg ctggcgagca gttcgtggag
     4861 caatgcggcg caggcgac
//
LOCUS       DAOSDX010000081         4857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6172_length_4857_cov_4.200750, whole genome shotgun sequence.
ACCESSION   DAOSDX010000081 DAOSDX010000000
VERSION     DAOSDX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6172_length_4857_cov_4.200750"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /gene="dmpH"
                     /locus_tag="PLN02_02735"
     CDS             complement(<1..603)
                     /gene="dmpH"
                     /locus_tag="PLN02_02735"
                     /EC_number="4.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047437 - 4-oxalocrotonate decarboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-3-hexenedioate decarboxylase"
                     /protein_id="HOI51265.1"
                     /translation="MKLNQNTIAALAELLENAELQAHDVVKITDAHPEMDWDDAYAIQ
                     NEILRRKLARGNKVVGLKCGLTSHAKMKQMGVDTPCFGFLVDYFTVPDGGEVKIKELI
                     HPKVEPEIVFVLKKALSGPGCHVGSVLAATDFVMAGIEVIDSRYRDFKFDLKSVIADN
                     CSSSRYVLGGMAVPVADLDLRTLGVVMEKNGQVVSIGAGAA"
     gene            complement(600..1631)
                     /gene="dmpG"
                     /locus_tag="PLN02_02740"
     CDS             complement(600..1631)
                     /gene="dmpG"
                     /locus_tag="PLN02_02740"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HOI51266.1"
                     /translation="MTLQGKKITVHDMTLRDGMHPKRHLMTLDQMVSIASGLDEAGIP
                     LIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPKMKQAKVSALLLPGIGTVDHLKMAR
                     DLGVNTIRVATHCTEADVSEQHITLARKLEMDTVGFLMMAHMNSPEGLVKQAKLMESY
                     GANCIYVTDSAGHLLPDTVKARLSAVRAALKPETELGFHGHHNLAMGVANSIAAIEVG
                     ANRIDAAAAGLGAGAGNTPMEVLVAVCSLMGIETGVDVAKITDVAEDLVVPIMDFPIR
                     IDRDALTLGYAGVYGSFLLFAKRASAKYGVPAREILVELGRRGMVGGQEDMIEDTAIT
                     MARERGLIA"
     gene            complement(1643..2560)
                     /locus_tag="PLN02_02745"
     CDS             complement(1643..2560)
                     /locus_tag="PLN02_02745"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HOI51267.1"
                     /translation="MTQKIKCALIGPGNIGTDLLYKLKRSPFLEPAWMVGIDPESEGL
                     KRAAEMGLKVTAGGVDGLLPHVLEDNIQIAFDATSAYVHAENSRKLNELGVLMIDLTP
                     AAIGPYCVPPVNLVEHVGKREMNVNMVTCGGQATIPMVAAVSRVQPVAYGEIVATVSS
                     KSAGPGTRKNIDEFTRTTAGAVEKVGGAKKGKAIIIINPAEPPLIMRDTVHCLTETAP
                     DQAKITESIHAMIKEVQKYVPGYRLVNGPVFDGNRVSVYLEVQGLGDFLPTYAGNLDI
                     MTAAGARTAEMFAEEMIKGTLKLAPTQAA"
     gene            complement(2584..3378)
                     /locus_tag="PLN02_02750"
     CDS             complement(2584..3378)
                     /locus_tag="PLN02_02750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI51268.1"
                     /translation="MDLQINGKTALVTGSTAGIGFAIAAELAREGAVVTINGRSQAGV
                     DAALGRLREQVPGARVSGVAADLATAAGCAELFAACPRAEIVVNNLGIFDPAPFEAIA
                     DSEWQRFFDTNVMSGVRVARHYLPAMKAANWGRIVFISSESGINTPGEMVHYGMTKSA
                     QLAVSRGLAQSCAGTGVTVNAVLPGPTRSEGVTDFFGKLAADAGIAVDEMERRFFAEG
                     RPTSILKRMIAPAEVAALVAYVCSARASATTGAALRVEGGVVSTMC"
     gene            complement(3389..4171)
                     /gene="dmpE"
                     /locus_tag="PLN02_02755"
     CDS             complement(3389..4171)
                     /gene="dmpE"
                     /locus_tag="PLN02_02755"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017243903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxopent-4-enoate hydratase"
                     /protein_id="HOI51269.1"
                     /translation="MEQAQILQLGDELYEALASRQTISPLTSRGFDITIEDAYHIQQQ
                     MLARRLANGEKVIGKKIGVTSKVVMDMLGVYQPDFGYLLDGMVYNEGESIPMDTLIQP
                     KAEGEIAFVLKKDLMGPGVTNADVLAATEGVMACFEIVDSRIQDWKIKIQDTVADNAS
                     CGVFVLGDRLVDPKCLDLTMCGMVVEKNGEIIGTGAGAAALGSPINAVAWLANTLGKL
                     GIPLKAGEVILSGSLAPLFPVKAGDSLRVTVGGIGGCSVRFV"
     gene            complement(4189..>4857)
                     /locus_tag="PLN02_02760"
     CDS             complement(4189..>4857)
                     /locus_tag="PLN02_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI51270.1"
                     /translation="LGKTCRAIAPDMVGFGYTEPPAGIDYNIDAWVAHAVGLLDALGI
                     ERTDLIGNSFGGAIALALAIRHPQRVRRLVLMGSVGVPFAITPELDAVWGYQPSLAAM
                     RGLLDIFAHDRGLVTDELAELRYQASIRPGFQESFAAMFPAPRQRWVDAMASAEADIR
                     AIQHKTLIIHGRDDRVIPLSNSQTLNRWIDDSQLHVFGRCGHWVQIEHSADFNHLVGH
                     FLAD"
BASE COUNT          758 a         1552 c         1640 g          907 t
ORIGIN      
        1 cgcggccccg gcaccgatcg agacgacctg gccgttcttc tccatcacca cgcccagcgt
       61 gcgcaggtcg agatcggcca ccggcaccgc catgccgccc agcacatagc gcgacgacga
      121 gcagttgtcg gcgatcacgc tcttcaggtc gaacttgaag tcacggtagc gcgagtcgat
      181 gacctcgatg ccggccatca cgaaatcggt cgcggcgagc accgagccga catggcagcc
      241 cggcccgctc agcgccttct tcaatacgaa gacgatttcc ggctcgacct tggggtggat
      301 cagttccttg atcttcactt cgccgccgtc gggcaccgtg aagtaatcga ccaggaaacc
      361 gaagcacggc gtgtcgacgc ccatctgctt catcttggcg tgcgaggtca ggccgcactt
      421 gaggccgacc accttgttgc cgcgcgccag cttgcggcgc aggatctcgt tctggatcgc
      481 gtaggcgtcg tcccagtcca tctcgggatg ggcgtcggtg atcttgacga cgtcgtgcgc
      541 ctgcagctcg gcgttttcca gcagttcagc cagcgcggcg atggtgttct ggttcagttt
      601 catgcgatca gtccccgttc tcttgccatc gtgatggcgg tgtcttcaat catgtcttcc
      661 tggccgccga ccatgccgcg ccggcccagt tcgaccagga tttcgcgggc cggcacgccg
      721 tacttggcgg aagcgcgctt ggcgaacagt aggaaggagc catagacgcc ggcgtaaccc
      781 agggtcaggg cgtcgcggtc gatgcggatc gggaagtcca tgatcggcac caccaggtct
      841 tcggcaacgt cggtgatctt ggcgacatcg acgccggtct cgatccccat caggctgcac
      901 acggcgacca gcacttccat cggcgtgttg ccggcaccgg caccgagccc ggcggccgcc
      961 gcgtcgatgc ggttggcgcc gacctcgatc gccgcgatgc tgttggccac gcccatcgcc
     1021 aggttgtggt ggccgtggaa accgagttcg gtttccggct tcagcgccgc gcgcaccgcg
     1081 ctcaggcggg ccttgacggt gtccggcagc aggtggccgg cggagtcggt gacgtagatg
     1141 cagttggcgc cgtaggactc catcagcttg gcctgcttga ccaggccttc cgggctgttc
     1201 atgtgggcca tcatcaggaa gccgacagtg tccatctcca gcttgcgcgc cagcgtgatg
     1261 tgctgctcgg agacgtcggc ctcggtgcag tgggtggcga cgcggatggt gttgacgccg
     1321 aggtcgcgcg ccatcttcag gtgatcgacg gtgccgatgc ccggcagcag cagcgccgag
     1381 accttggcct gcttcatctt cggaatcacc gcgccgaggt attcctcgtc ggtgtgtgcc
     1441 gggaagccat aattgaccga cgagccgccg aggccgtcgc cgtgggtgac ttcgatcagc
     1501 ggaatgccgg cttcgtcgag tccgctggcg atgctgacca tctggtcgag cgtcatcagg
     1561 tggcgcttgg ggtgcatgcc gtcacgcagc gtcatgtcgt ggacggtgat tttcttgccc
     1621 tgcagtgtca tctcgtttct cctcaggcag cttgggtcgg cgcaagcttg agcgtgccct
     1681 tgatcatttc ttcggcgaac atctcggcgg tgcgcgcccc ggcggcggtc atgatgtcca
     1741 ggttgccggc gtaggtcggc aggaaatcgc ccaggccctg cacttccagg tagacggaaa
     1801 cgcggttgcc gtcgaacacc gggccattga ccagacggta gcccggcacg tatttctgca
     1861 cttccttgat catggcgtgg atcgactcgg tgatcttggc ttggtccggt gccgtttcgg
     1921 tcaggcaatg cacggtgtcg cgcatgatca gcggcggctc ggccgggttg atgatgatga
     1981 tcgccttgcc cttcttcgcc ccgccgacct tctcgacggc accggcggtg gtgcgggtga
     2041 attcgtcgat gttcttgcgc gtgcccggac cggccgactt ggacgagacg gtggcgacga
     2101 tttcgccgta ggccaccggc tgcacgcggg acaccgcggc gaccatgggg atcgtcgcct
     2161 ggccgccgca ggtgaccatg ttgacgttca tctcgcgctt gccgacatgc tcgaccagat
     2221 tgaccggcgg cacgcagtag ggaccgatgg cggccggcgt caggtcgatc atcagcacgc
     2281 cgagttcgtt cagtttgcgg ctgttctcgg catggacgta ggccgaggtc gcgtcgaagg
     2341 cgatctggat gttgtcttcc aggacgtgcg gcagcaggcc gtcgacgcca ccggcggtga
     2401 ctttgaggcc catctcggcg gcccgcttga ggccttccga ttcgggatcg ataccgacca
     2461 tccaggccgg ttcgaggaag gggctgcgct tgagtttgta gagcagatcg gtgccgatat
     2521 tgccgggacc gatcagcgcg catttgattt tttgggtcat ggggatagtc cttgttgtca
     2581 gggtcaacac atggtggaaa cgacgccgcc ctcgacgcgc agcgccgcgc cggtcgtcgc
     2641 cgacgctcgc gcgctgcaga cgtaggcgac cagcgccgcc acttcggcgg gggctatcat
     2701 tcgtttgagg atcgaggtcg gccggccctc ggcgaagaag cggcgctcca tctcgtcgac
     2761 agcgatgccg gcgtcggcgg cgagcttgcc gaagaagtcg gtaacgcctt ccgagcgggt
     2821 cggtccgggc agcacggcat tgaccgtgac gccggtgccg gcacagctct gggccaggcc
     2881 gcgggaaacg gcgagctggg ccgacttggt catgccgtaa tgcaccattt cgcccggcgt
     2941 gttgatgccg gattcgctgg agatgaagac gatgcggccc cagttggcgg ccttcatcgc
     3001 cggcaggtag tgacgggcga cgcgcacgcc gctcatcacg ttggtgtcga agaagcgctg
     3061 ccactcgctg tcggcaatcg cctcgaaggg cgccgggtcg aagatgccga ggttgttcac
     3121 gacgatctcg gcccgcggac aggcggcaaa cagttcggcg cagccggcgg ccgttgccag
     3181 gtcggcggca acaccgctga cgcgggcgcc cggcacctgc tcacgcaggc ggccgagcgc
     3241 cgcatcgacg ccggcctgac tacggccatt gatggtgacc acggccccct cgcgcgccag
     3301 ttcggcggcg atggcgaagc cgatgccggc ggtcgagccg gtcaccagcg ccgtcttgcc
     3361 gttgatctgc aagtccattg ccgctgcctc agacgaaacg cacggagcag ccgccgatgc
     3421 cgccgacggt gacgcgcagg ctgtcgccgg ccttgaccgg gaacagcggc gccagcgaac
     3481 cagagaggat gacctcgccg gccttcagcg gaatgccgag cttgcccagg gtattggcca
     3541 gccaggcgac ggcgttgatc ggcgaaccga gcgcggcggc gccggcaccg gtgccgatga
     3601 tttcgccgtt tttctcgacg accatgccgc acatcgtcag gtcgaggcac ttcggatcga
     3661 ccaggcggtc gccgagcacg aagacgccgc acgaagcgtt gtcggcgacg gtgtcctgga
     3721 tcttgatctt ccagtcctgg atgcgcgaat cgacgatctc gaagcaggcc atgacgcctt
     3781 cggtggcggc cagcacgtcg gcgttggtaa cacccggccc catcaggtcc ttcttcagca
     3841 cgaaggcgat ctcgccttct gccttgggct ggatcagcgt gtccatcggg atcgactcgc
     3901 cctcgttgta caccatgccg tcgagcaggt agccgaagtc cggctgatag acgccgagca
     3961 tgtccatcac caccttgctg gtgacgccga tcttcttgcc gatgaccttt tcgccattgg
     4021 ccaggcggcg ggccagcatc tgctgctgga tgtggtaggc gtcctcgatc gtgatgtcga
     4081 agccgcgcga ggtcagcggg ctgatcgtct ggcggctggc cagcgcctcg tagagttcgt
     4141 cgcccagttg caggatttgt gcttgttcca tcgtcttgtt tcctcgtttc agtcggccag
     4201 gaaatggccg accagatggt tgaaatcggc gctgtgctcg atctgcaccc agtggccgca
     4261 gcggccgaag acgtgcagtt gcgagtcgtc gatccagcgg ttcagggtct gcgagttgct
     4321 cagcggaatg acccggtcgt cgcggccgtg aatgatcagc gtcttgtgct ggatcgcccg
     4381 gatgtcggcc tcggcgctgg ccatcgcatc gacccagcgc tgacgcgggg ccgggaacat
     4441 cgccgcgaac gattcctgga agccgggccg gatgctcgcc tggtagcgca gctcggccag
     4501 ttcgtcggta accagcccgc ggtcgtgggc gaagatgtcg agcaaaccgc gcatcgccgc
     4561 cagtgacggc tggtagcccc agaccgcgtc aagttccggc gtgatggcaa agggcacgcc
     4621 gacgctgccc atcagcacca gccggcgcac ccgttgcgga tgacggatgg ccagcgccag
     4681 cgcgatggcg ccgccgaagg agttgccgat caggtcggtc cgctcgatgc ccagcgcgtc
     4741 aagcaggccg accgcgtgcg ccacccaggc gtcgatgttg tagtcgatgc cggccggcgg
     4801 ctcggtgtag ccgaagccga ccatgtcggg cgcaatcgcc cggcaggtct tgcccag
//
LOCUS       DAOSDX010000082         4831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6223_length_4831_cov_3.659548, whole genome shotgun sequence.
ACCESSION   DAOSDX010000082 DAOSDX010000000
VERSION     DAOSDX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6223_length_4831_cov_3.659548"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..492)
                     /locus_tag="PLN02_02765"
     CDS             complement(13..492)
                     /locus_tag="PLN02_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51271.1"
                     /translation="MDDFSALWLARAAHVIGVVLWIGGVAFVTTTLLPAIRRDFPVAA
                     QLAEFERYERGFGRQARWTTQLVGGSGLFMVWKLDLWFRFATPDAFWMHAMLAIYLLF
                     TLLLFVIEPLFFHRLLRHLAQRDPALPPRLLGRLHWLLLTLSLLTTGFAVAGAHGWG"
     gene            complement(492..2033)
                     /locus_tag="PLN02_02770"
     CDS             complement(492..2033)
                     /locus_tag="PLN02_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOI51272.1"
                     /translation="MTARLLLCLAIVWPLFTSAAEPAALYRQHCASCHGADRLGGIGP
                     ALLPESLERLRRPEAEKTIRDGRIATQMPAFATSFDDAQVKALADYLYQPVTPAPAWS
                     EADIRASRIEHVDPATLADHPVFSADPLNLFVVVESGDHHVSILDGDRLEPIHRFASR
                     YALHGGPKFSPDGRYVYFASRDGWLSKFDLWNLTTVAEVRAGLNTRNAAVSGDGRLVI
                     VANTLPGNLVVFDNELRLVKTIAAASLDGRQSSRVSAVYDASPRQSFIAALKDLPEVW
                     EISYDEQAAPIHDGLVHDYRMGEAIARPGYLHPRRTPLDTVLDDFFFDQSYTHVMGAS
                     RDGKGQVVNLDIRRKVADLALPGMPHLGSGITFTWQGKPVMASTNLKTGEVSVIGMSD
                     WQLVKAIPTRGPGFFLRSHSQSRYAFVDSMMSREGRNHLQLIDKESLEVVREFTAPPG
                     QTLAHVEFTRDGRYALASLWEMDGALVVYDMQTLQEVKRLPMKKPVGKYNVWNKTRKD
                     EGTSH"
     gene            complement(2030..3223)
                     /gene="nirJ"
                     /locus_tag="PLN02_02775"
     CDS             complement(2030..3223)
                     /gene="nirJ"
                     /locus_tag="PLN02_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HOI51273.1"
                     /translation="MFRISQFLREIDQPTPAGPRRHPPGPVVIWNLLRRCNLTCKHCY
                     SISADKHFPGELSTDEINSVMDDLRAFRVPVLILSGGEPLLHPDIYSIASRAKRLGFY
                     VALSTNGTLIDRDNIDLIDAIAFDYVGISLDGMAATHDRFRRQEGAFAASLAALRLCR
                     ERRIKVGVRYTMTEDNAHDLPALLQLVADEGIDKFYFSHLNYAGRGNKHRADDARHAS
                     TRAAMDLLFDTCLDSLRRGEDKEFVTGNNDADGVYLLHWAARRCPQSLPHLLAKLRQW
                     GGNASGVNVANIDNLGNVHPDTMWWHQTLGNVRERPFSAIWSDPANPLLERLREHPRR
                     LEGRCAGCRYLAICNGSSRVRAEQVTGNPWAEDPGCYLDDAEIGGAASERIRTTPWTR
                     LEKVA"
     gene            complement(3227..3745)
                     /locus_tag="PLN02_02780"
     CDS             complement(3227..3745)
                     /locus_tag="PLN02_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOI51274.1"
                     /translation="MNAPTAPDAFERRLVRVTQEGLPLVPRPYASIAAVLGVSEAQVI
                     ERLQAMLERGAIRRIGAVPNHYAIGYTANGMSVWDIDDDAVDRVGELLGRQAAVSHCY
                     RRPRRLPDWPYNLFAMLHGRQRPEVDAQLAALAALVERECGAVVRARDILFSSRILKK
                     TGLRLGAGPRET"
     gene            complement(3742..4194)
                     /locus_tag="PLN02_02785"
     CDS             complement(3742..4194)
                     /locus_tag="PLN02_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HOI51275.1"
                     /translation="MAELDAIDRAIINALQGDFPLCPAPFAAAAAPLGLNEGELLERL
                     RALRDAGTLSRFGPLYQIERLGGAFCLAAMAVPEADFERVAGIVNGFAEVAHNYRRDH
                     RLNMWFVLATETPAEIAATCAAIEAASGLPVLPFPKEREYFVDLRLQA"
     gene            complement(4187..>4831)
                     /locus_tag="PLN02_02790"
     CDS             complement(4187..>4831)
                     /locus_tag="PLN02_02790"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOI51276.1"
                     /translation="ARIAAETDCPLLELPLQTAYHIDLGFDLAAPPGHRPAAPAPATG
                     GAPACALDGIDRALLGALADGLPLCAHPYAPVAAAAGVSEAWLLSRLAEWLDSGLLKR
                     FGVVVRHHELGYVANAMCVWNVPEERIDALGRELAREPGVTLCYRRRRQMPDWPYNLY
                     CMIHGRSREGVLQLRDALAGRTGLDRHPGQVLFSRRRFKQCGARYQLSAGACHG"
BASE COUNT          695 a         1468 c         1904 g          764 t
ORIGIN      
        1 gtaatactgt tctcaacccc acccatgcgc cccggctacc gcgaagccgg tggtgagcag
       61 gctgagggtg agcagcagcc agtgcaggcg gccgaggagg cgtggcggca gggcggggtc
      121 gcgttgcgcg aggtggcgaa gcaggcggtg gaagaagagc ggttcgatga cgaagaggag
      181 cagcgtgaag agcaggtaga tggccagcat cgcgtgcatc cagaaggcgt cgggggtggc
      241 gaagcggaac cagaggtcga gtttccagac catgaacagg ccgctgccgc cgaccagttg
      301 cgtggtccag cgcgcctggc ggccgaagcc gcgttcgtag cgttcgaatt cggcgagctg
      361 ggcggcgacc gggaagtcgc ggcggatcgc cggcagcagc gtggtggtga cgaaggcgac
      421 gccgccgatc cacagcacga cgccgatcac gtgcgcggcg cgggccagcc agagggcgct
      481 gaagtcgtcc atcagtggct ggtcccttcg tccttgcgcg tcttgttcca gacgttgtac
      541 ttgccgaccg gtttcttcat cggcaggcgc ttcacctcct gcagcgtctg catgtcgtag
      601 acgaccagcg cgccgtccat ttcccacagg ctggcgagcg cgtagcggcc gtcgcgggtg
      661 aattcgacgt gggccagcgt ctggccgggc ggggcggtga attcgcgcac cacctcaagg
      721 ctttccttgt cgatcagttg caggtggttg cggccttcgc ggctcatcat cgaatcgacg
      781 aaggcgtagc gcgactggct gtggctgcgc aggaagaagc cggggccgcg ggtcgggatg
      841 gccttgacca gctgccagtc gctcatgccg atgacgctga cttcgccggt cttcaggttg
      901 gtcgaggcca ttaccggctt gccctgccag gtgaaggtga tgccggagcc gaggtggggc
      961 atgccgggta gcgccaggtc ggcgaccttg cgccggatgt cgaggttgac cacctgccct
     1021 ttgccgtcgc ggctggcgcc catgacgtgg gtgtagctct gatcgaagaa gaaatcgtcg
     1081 agcacggtgt cgagcggcgt ccgccgcgga tgcaggtagc cggggcgggc aatcgcctcg
     1141 cccatccggt agtcgtgcac caggccgtcg tggatcggcg ccgcctgctc gtcgtagctg
     1201 atttcccaga cttccggcag gtccttcagc gcggcgatga aactctggcg cggcgaggcg
     1261 tcgtacaccg ccgagacgcg cgagctctgg cggccgtcca ggctggcggc ggcgatcgtc
     1321 ttgaccaggc gcagctcgtt gtcgaagacc accaggttgc cgggcagggt gttggcgacg
     1381 atgaccaggc gaccgtcgcc ggagacggcg gcattgcgcg tgttgaggcc ggcgcggact
     1441 tcggcgacgg tggtcaggtt ccacaggtcg aatttgctca gccagccgtc gcgcgaggcg
     1501 aaatagacgt agcggccgtc cggcgagaac ttggggccgc cgtgcagggc gtagcgcgag
     1561 gcgaagcggt ggatcggttc gaggcggtcg ccgtcgagga tgctgacgtg gtggtcgccg
     1621 ctttcgacga cgacgaagag gttgagcggg tcggcgctga acaccggatg gtcggcgagt
     1681 gtcgccggat cgacgtgctc gatgcgcgag gcgcggatgt cggcctcgct ccaggccggc
     1741 gccggcgtca ccggctggta gaggtagtcg gccagcgcct tgacctgggc gtcgtcgaag
     1801 ctggtggcga aggccggcat ctgggtggcg atgcggccgt cgcggatggt cttttcggct
     1861 tccgggcggc gcaggcgttc gaggctttcc ggcagcagcg ccgggccgat gccgccgagg
     1921 cggtcggcac cgtggcagga agcgcagtgc tggcggtaga gggcggcggg ttcggcggcg
     1981 ctggtgaaga gtggccagac gatggcgagg cagagcagga ggcgggcggt catgcgactt
     2041 tctccaggcg ggtccacggc gtggtgcgga tgcgttcgct ggcggcgccg ccgatctcgg
     2101 cgtcgtcgag gtagcagccg gggtcttcgg cccatggatt gccggtgacc tgctcggcgc
     2161 gcacgcgcga actgccgttg cagatggcga ggtagcggca gccggcgcag cgcccttcga
     2221 ggcggcgcgg gtgttcgcgc aggcgctcga gcagcgggtt ggccgggtcg ctccagatgg
     2281 cggagaaggg ccgctcgcgg acgttgccca gggtctggtg ccaccacatg gtgtccgggt
     2341 gcacgttgcc caggttgtcg atgttggcga cgttgacgcc gctggcgttg ccgccccact
     2401 ggcgcagctt ggccagcagg tggggcaggc tttgcgggca gcggcgggcc gcccagtgca
     2461 gcaggtagac gccgtcggcg tcgttgttgc cggtgacgaa ttccttgtcc tcgccgcgcc
     2521 gcaggctgtc caggcaggtg tcgaacagga ggtccatggc ggcgcgcgtg ctggcgtggc
     2581 gggcgtcgtc ggcgcggtgc ttgttgccgc ggccggcata gttgaggtgc gagaagtaga
     2641 acttgtcgat gccctcgtcg gcgaccagct gcagcagcgc cggcaggtcg tgggcgttgt
     2701 cctcggtcat cgtgtagcgg acgccgacct tgatgcgccg ttcgcggcac agccgcagcg
     2761 ccgccagcga ggcggcgaag gcgccttcct ggcggcggaa gcggtcgtgc gtggcggcca
     2821 tgccgtcgag gctgatgccg acgtagtcga aggcgatggc gtcgatcagg tcgatgttgt
     2881 cgcggtcgat cagggtgccg ttggtcgaca gcgcgacgta gaagccgagg cgcttggcgc
     2941 ggctggcgat gctgtagatg tccgggtgca gcagcggctc gccgccggaa aggatcagca
     3001 ccggcacgcg gaaggcgcgc aggtcgtcca tcacggagtt gatttcgtca gtgctcagct
     3061 cgccggggaa gtgcttgtcg gcggagatcg agtagcagtg cttgcaggtc aggttgcagc
     3121 gacgcagcag gttccagatc accaccgggc cgggcgggtg gcggcgcgga ccggccggcg
     3181 tcggctgatc gatttccctg aggaactggc tgatgcggaa catggttcag gtctcccggg
     3241 ggccggcgcc aaggcgcagg ccggttttct tgaggatgcg gctggaaaac aggatgtcgc
     3301 gcgcgcgcac cacggcgccg cattcgcgct cgaccagcgc ggccagcgcc gccagttggg
     3361 cgtccacttc ggggcgctgg cgaccgtgca gcatggcaaa caggttgtac ggccagtccg
     3421 gcaggcggcg cggccggcgg tagcagtggc tgaccgccgc ctgccggccg agcagctcgc
     3481 cgacccggtc gacggcgtcg tcgtcgatgt cccagacgct catgccgttg gccgtgtagc
     3541 caatggcgta gtggttgggc acggcgccga tgcggcggat ggcgccgcgt tcgagcatcg
     3601 cctgcaggcg ctcgatgacc tgcgcctcgc tgacgccgag gacggcggcg atgctggcgt
     3661 aagggcgcgg caccagcggc aggccttcct gggtgacgcg caccaggcgg cgttcgaagg
     3721 cgtcgggggc ggtgggcgcg ttcatgcctg caacctcagg tcgacgaagt attccctttc
     3781 cttgggaaag ggcagcaccg gcaagccgct cgccgcctcg atggcggcac aggtggcggc
     3841 gatctccgcc ggcgtctcgg tggcgagcac gaaccacatg ttgaggcggt ggtcgcgccg
     3901 gtagttgtgg gcgacttcgg caaagccgtt gacgatgccg gcgacccgct cgaagtccgc
     3961 ttccggcacc gccatcgccg ccaggcagaa ggcgccgccg aggcgttcga tctggtagag
     4021 cgggccgaag cggctcaggg tgccggcgtc gcgcagggcg cgcaggcgtt ccagcagttc
     4081 gccctcgttc agtccgagcg gcgccgcggc ggcggcgaag ggcgccgggc agagcgggaa
     4141 atcgccctgc agggcgttga tgatggcgcg gtcgatggcg tcgagctcag ccatggcagg
     4201 ctccggcgga aagttggtag cgggcgccgc actgcttgaa gcggcggcgg ctgaacagca
     4261 cctggccggg gtggcggtcg agcccggtgc gcccggccag cgcgtcgcgc agttgcagca
     4321 cgccctcgcg gctgcggccg tggatcatgc agtagaggtt gtacggccag tccggcatct
     4381 gccgccgccg gcgatagcac agggtgacgc cgggttcgcg tgcgagttcg cggccgagcg
     4441 cgtcgatgcg ttcttccggg acgttccaga cgcacatcgc gttggctacg tagccgagtt
     4501 catggtgccg gacgacgacg ccgaagcgct tgagcaggcc gctgtccagc cattcggcga
     4561 gacgcgacag cagccaggcc tcgcttacgc cagcggcggc ggcgaccggc gcgtaggggt
     4621 gcgcgcacag cggcaggccg tcggccaggg cgccgagcag ggcgcggtcg atgccgtcca
     4681 gagcacaggc cggggcaccg cccgtcgccg gcgccggggc ggccggccgg tggccgggcg
     4741 gagcggcgag atcgaagccg aggtcgatat ggtaggcggt ctgcagcggc agttcgagca
     4801 gcgggcagtc agtttcggca gcgatccggg c
//
LOCUS       DAOSDX010000083         4824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6234_length_4824_cov_3.130426, whole genome shotgun sequence.
ACCESSION   DAOSDX010000083 DAOSDX010000000
VERSION     DAOSDX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6234_length_4824_cov_3.130426"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLN02_02795"
     CDS             <1..476
                     /locus_tag="PLN02_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOI51277.1"
                     /translation="EQGIPFVFDPGQGLPMFGGDDLMHFLRLADYACFNDYEAKLACD
                     RTGRSLEQLASEVRALIVTRGGEGSEIHADGARYDIPCVAPDAIVDPTGCGDAYRAGL
                     LYGIANGWDWTNTGRLASVMGSIKIAHRGGQNHQPSRAEIARRYSQAFGELPWQD"
     gene            557..1318
                     /locus_tag="PLN02_02800"
     CDS             557..1318
                     /locus_tag="PLN02_02800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOI51278.1"
                     /translation="MENLHRATQRSRPERKHLAVGFARSASDILDAQRLRHRVFAGEM
                     GANLPSRTPGVDHDIYDPYCEHLVVRDTQSGEIVGTYRILPPENARQVGYYSENEFDL
                     TRLQHLRSRLVEIGRSCVHPDYRTGATITLLWSGLAQYMTEHGHDYLIGCASISMADG
                     GHAAASLYNRLVVDHLGPQEYRVFPRCPLPLAALQQDRPAEVPPLIKGYLRAGAWICG
                     EPAWDPDFNTADLPILMPMAKVSNRYAKHYLGQAD"
     gene            1519..1839
                     /locus_tag="PLN02_02805"
                     /pseudo
     CDS             1519..1839
                     /locus_tag="PLN02_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            complement(2030..2860)
                     /locus_tag="PLN02_02810"
     CDS             complement(2030..2860)
                     /locus_tag="PLN02_02810"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HOI51279.1"
                     /translation="MSTYAIGDIQGCHDSLLRLLELCAFDPAQDRLWLVGDLVNRGPK
                     SLETLRLIKSFGDSAVTVLGNHDLYLLMVAEGGAKYRAKDDTLDDVLAALDRDELLDW
                     LRRQPLCHTEGDFCLVHAGLLPQWTAKRARELAREVEARLQGKDYRDFILNLWGSEPA
                     AWEDDLKGWGRLRVIVNAMTRMRFCTKKGVMEFKVKGKLNNAPAGYLPWFEIPGRKSA
                     DSVLVTGHWSALGLRITQNLLALDSGCLWGGHLTAVRLEDRRVFQVDCSPAEALPLKQ
                     "
     gene            complement(2983..>4824)
                     /gene="pbpC"
                     /locus_tag="PLN02_02815"
     CDS             complement(2983..>4824)
                     /gene="pbpC"
                     /locus_tag="PLN02_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOI51280.1"
                     /translation="YLSYAPMGGVLEGVEAASRAYLGKPAASLSHGDAALLAVLPQAP
                     SRLRPDRYPAAARAARDKVLDRLVPRWGEVTVADARQEAVIAQTVREPLLAPLFAERV
                     RKLHPGVARIETTLDAATQSSVEQLLGSRLGVLPPRVSMAALVVDNATLEVRAYAGSA
                     DFSDNQRFAHVDMVLGRRSPGSALKPFLYGLAFDEGLIHSESLLSDVPQSFGGYQPGN
                     FQASFSGPVGVTEALQRSLNVPAVEVLDRLGPQRFVGLLRAGGLKLDLPRDAQPNLSV
                     ILGGVAVSLEDLVGAYTAFARGGVAGRPRYTTTSPVEERRMLSEGAAFIIRDLLEAGG
                     PVGRQVEAGVGVRRGIAWKTGTSFGFRDAWAIGVSDRHTVGVWVGRPDGTPNPGFFGA
                     NIAAPLLVDIFSAIEAPGRVDRPPPASVSQARICWPLGLRSADTPAELCHQERSAWLL
                     NGAAPPTFSDRLRSGAARYTDYRDASSGLRVRAACAGGEMFAVDMARWPAALEPWLDA
                     TTRSRALPPPWSAECGQAQAPGDGLKIVGVARGETIRRAAGGAAPLLRLEARGSRQAL
                     IWLVDGRQVGRTAPGKALQQRFAEPGRHTITVMDDSGRYDRVEISVQ"
BASE COUNT          788 a         1713 c         1594 g          729 t
ORIGIN      
        1 acgaacaagg catccccttc gtcttcgatc cgggccaggg cctgccgatg ttcggcggcg
       61 acgacctgat gcatttcctg cgcctcgctg actacgcctg cttcaacgac tacgaggcca
      121 agctcgcctg cgaccgcacc gggcgcagcc tcgaacaact ggcgagcgag gtaagagcgc
      181 tgatcgtcac ccgcggcggc gaaggctcgg aaatccacgc cgacggcgcc cgttacgaca
      241 ttccctgcgt cgcgcccgac gccatcgtcg accccaccgg ctgtggcgac gcctatcgcg
      301 ccggcctcct ctacggcatc gccaacggct gggactggac taacaccggt cgcctggctt
      361 ctgtgatggg atcgatcaag atcgcccacc gcggcggcca gaaccaccag ccgagccgtg
      421 ccgaaatcgc ccgccgctac agccaggcct tcggcgaact cccctggcag gactgaaacc
      481 gcgacgtcat ccgcgggaaa tcggcgtgta acacgcctgt tccatgctgt tctccactgc
      541 ctacaaggag aacaacatgg aaaacctgca ccgcgctacg cagcgcagcc gtcccgaacg
      601 caagcatctg gccgtcggct ttgcccgcag cgccagcgac atcctcgacg cgcaacgtct
      661 gcgccaccgc gtcttcgctg gcgaaatggg cgccaacctg ccgagccgca cgcccggcgt
      721 cgaccacgac atctacgatc cctactgcga acacctggtc gtgcgcgaca cgcaaagcgg
      781 cgaaatcgtc ggcacctacc gcatcctgcc gccggaaaac gcccgccagg tcggctacta
      841 ctcggaaaac gaattcgacc tcacccgcct ccagcacctg cgctcccgcc tcgtggaaat
      901 cggccgctcc tgcgtccatc ccgattaccg gaccggcgcg acgatcacct tgctctggtc
      961 gggcctcgcc cagtacatga ccgaacacgg ccacgactac ctgatcggct gcgcctcgat
     1021 cagcatggcc gacggcggcc atgccgcggc cagcctgtat aatcgcttgg ttgtcgatca
     1081 tctcggaccg caggaatacc gcgtctttcc gcgctgtccg ctgccgctgg cggcgctgca
     1141 gcaggatcgc ccagccgagg tgccgccgct gatcaagggt tacctgcgcg ccggcgcctg
     1201 gatctgcggc gaaccggcct gggatcccga tttcaacacg gccgacctgc ccatcctgat
     1261 gccgatggcc aaggtttcca accgttacgc caaacactat ctgggacagg cagactgaac
     1321 gagaccaccg cccttcgccg cgccttccgg ctgttccggc tctttctgct ggtactggcc
     1381 ggcgcgctgg tgtcgttgac cgtcttccgc atttgcggcg acacgcgccg actgcggctc
     1441 aaacaaagct ggtcggcggc ggtgctcgac gcgctcggcg tcgaactgga agccgacctg
     1501 acccacctgg cacccggcgt gctgttggtc gccaaccatg tttcctggat cgacatcttc
     1561 gtcatcaacg ccgccgtgcc ggccgccttc gtctccaagg aagaagtccg gcgctggccg
     1621 gtgatcggct ggctggcctg gaagaacgat accgtcttcc tgcgtcgcgg cagccgcggc
     1681 cacgcccgcc tgatcaacga ggaaatcgcc ggcatcctgg ggcagggcaa ggtggttgcc
     1741 gtcttccccg aaggcacgac gaccgacggc cgcagcctgc tgcatttcca cgccgcgctg
     1801 ctgcagccgg cgctggccgc cggccggccg gtgctgccgg cagcgatttc ctactgggaa
     1861 ctgaacggcg aacgcagcct ggcgccgcgt tacgacggcg atatctcgct cggcgaatgc
     1921 actaccgcca tcctcaagcg gcgccgcatc atcgcccgcc tggtgacgac gccgctccgc
     1981 ggtagtaacg gcgaggaccg caagcaggtt gccgccgccg cgcgcgaggc tattgcttta
     2041 gcggcagggc ttccgctggc gagcaatcca cctgaaacac ccgccggtct tccagccgca
     2101 ctgcggtcag atgaccgccc cacaagcacc ccgaatcgag cgccagcaga ttctgcgtga
     2161 tgcgcaggcc gagcgccgac cagtggccgg tgaccagcac gctgtcggcg ctcttgcggc
     2221 cggggatctc gaaccacggc aggtaaccgg ccggcgcgtt gttcagcttg cccttgacct
     2281 tgaactccat gacgcccttc ttggtgcaga aacgcatccg ggtcatcgcg ttgacgatca
     2341 cccgcagccg cccccagccc ttcaggtcgt cttcccaggc ggccggttcg ctgccccaca
     2401 ggttgaggat gaagtcgcgg taatctttgc cctgcagcct ggcctcgact tcgcgcgcca
     2461 gttcgcgggc gcgtttggcc gtccattgcg gcagcaggcc ggcgtgcacc aggcagaaat
     2521 cgccctcggt gtggcaaagc ggctgccggc gcagccagtc gaggagttcg tcgcggtcca
     2581 gcgcagcgag cacgtcgtcc agtgtgtcgt ccttggcccg atacttggca ccgccctcgg
     2641 caaccatcaa caggtagagg tcgtggttgc ccagcacggt caccgccgag tcaccgaaag
     2701 acttgattag gcgcagcgtt tcgagcgact tcggaccgcg attgaccagg tcgccgacca
     2761 gccacagccg gtcctgcgcc gggtcgaagg cgcagagctc gagcaggcgc aacagggaat
     2821 cgtggcaacc ctggatgtcg ccgatggcgt aagtactcat ggccgggact ccggcgggcg
     2881 ctggggtagg taagtcatct ctgcactggc cgtctggcgg tccgggtttg ttttatccga
     2941 acgataacaa atgccggcgg tttttgctgg ccggcgagat acctactgga cgctgatttc
     3001 cacccggtcg taacgaccgc tgtcgtccat caccgtgatc gtatgccgcc ccggctcggc
     3061 gaaacgctgc tgcagggcct tgccgggcgc cgtgcggccg acctgccgac catcgaccag
     3121 ccagatcagc gcctgccggc tgccgcgcgc ctccaggcgc agcagcgggg cggcgccgcc
     3181 ggcggcgcgg cggatcgtct cgccgcgggc gacaccgacg atcttcaggc catcgcccgg
     3241 cgcctgtgcc tggccgcatt ccgcactcca cggcgggggc agggcacggc tgcgggtcgt
     3301 cgcgtcgagc cagggttcga gcgccgccgg ccaacgcgcc atgtcgacag caaacatctc
     3361 gccgccggcg caggcggcgc gcacgcgcag gccgctgctg gcatcacggt aatcggtata
     3421 acgggcggca ccgctgcgca ggcggtcgga gaaggtcggc ggcgcggcgc cgttgagcag
     3481 ccaggcgctg cgttcctggt ggcagagttc ggccggcgta tcggcgctgc gcaggccgag
     3541 cggccagcag atgcgggcct ggctgacgct ggccggcggc ggccggtcga cgcggcccgg
     3601 cgcctcgatc gccgagaaga tatcgaccag cagcggcgcc gcgatgttgg cgccgaagaa
     3661 acccgggttg ggcgtgccgt ccgggcggcc gacccagacg ccgaccgtgt ggcggtcgct
     3721 gacgccgatt gcccaggcgt cgcggaaacc gaagctggtg ccggtcttcc aggcgatgcc
     3781 gcggcgtacg ccgacgccgg cctcgacctg gcggccgacc gggccgccgg cttcgagcag
     3841 gtcgcgaatg atgaaggcgg cgccttcgct cagcatccgc ctttcctcga ccggcgatgt
     3901 cgtcgtataa cgcggccgac cggcgacgcc gccgcgggcg aaagcggtgt aggcgccgac
     3961 caggtcttcg aggctgacgg cgacgccgcc gaggatcacc gacagattgg gctgggcgtc
     4021 gcgcggcagg tcgagtttca gcccgccggc ccgcaatagg ccgacgaagc gctgcgggcc
     4081 gagacggtcg aggacttcga ccgccggcac gttgagcgaa cgctgcaggg cttcggtgac
     4141 gccgaccggc ccggaaaagc tcgcctggaa attgcccggc tggtagccgc cgaaggactg
     4201 cggcacgtcg gaaagcagcg attccgaatg gatcagccct tcgtcgaaag ccagtccgta
     4261 gaggaagggc ttgagcgccg aacccggcga gcggcggccg agcaccatat caacgtgggc
     4321 gaaacgctgg ttgtcggaaa aatcggccga accggcgtag gcgcggactt ccagcgtcgc
     4381 gttgtcgacc accagggcgg ccatcgaaac gcgcggcggc agcacgccga gacggctgcc
     4441 gagcagttgc tcgacactgc tctgggtggc ggcgtcgagc gtcgtctcga tgcgggcgac
     4501 accgggatgc agcttgcgca cgcgctcggc gaacagcggc gcgagcagcg gctcgcgcac
     4561 ggtctgggcg atcaccgctt cctgccgggc gtcggcgacg gtcacctcgc cccagcgcgg
     4621 gaccaggcgg tcgagcacct tgtcgcgggc ggcgcgcgcc gccgccggat agcgatccgg
     4681 acgcagccgc gacggcgcct gcggcagcac cgcgagcagg gcggcgtcgc cgtgcgacaa
     4741 cgaggccgcc ggcttgccca ggtaggcacg gctggccgcc tcgacgcctt cgaggacgcc
     4801 gcccatcggc gcatagctca ggta
//
LOCUS       DAOSDX010000084         4809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6256_length_4809_cov_3.545646, whole genome shotgun sequence.
ACCESSION   DAOSDX010000084 DAOSDX010000000
VERSION     DAOSDX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6256_length_4809_cov_3.545646"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLN02_02820"
     CDS             complement(<1..1378)
                     /locus_tag="PLN02_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI51281.1"
                     /translation="MQISIKEHHRQYRHPYYLYAPDFTELSCGIRVMHYLCHILNLHG
                     YEAYVVTPKTSPELWTPCLTEAVINRHFALDLKPIVIYPEITRGTPLGVGVPVRWLLA
                     PPGKISGHADFAEGEITIGYREAFVDHPEIPLLTVPASDPRLFGPGLPEAERSGRYFF
                     FNRLLSSGGELLPVTDGAIEISPRQPRSLQELSDIFKTAELLYCYEEGAITVEARMCG
                     CPIVYLPNERWLPAMPVSDFSNDGIAWGDSEDEIRRAKATVHEIHRQALAIYQRFEPQ
                     FEAFVELTQNAAAAASMESCYSKSSRNIAGLPRNDLNAGIAQRQAGNLEGALLLLSRY
                     VSNFPDDGEAFFHLALVSADLGMFEHSASFVAEAMLLEPQRADYLAELARHLLRCGNP
                     RDAIRYFQEYQQRDPSDPSTYPELAEALYRNGQAQDALWMLSAASATSKQPEILAKLI
                     ELASRQGKL"
     gene            complement(1425..2354)
                     /locus_tag="PLN02_02825"
     CDS             complement(1425..2354)
                     /locus_tag="PLN02_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOI51282.1"
                     /translation="MFGQAILALAESCPELLVLSADLGNSSGLDRFKQTYPERYLNVG
                     IAEQNLIGVAGGLAKEGFTVFATSFAPFITMRAAEQIRMNLGYMGFNVKAVGIGSGLG
                     MGFLGNSHYGLEDAAIMRSIPNLTVVSPADCGAIAKTVFAAASHAGPMYIRLTGAPNT
                     PIVYREDFPFQLGRAIPLREGGDVALVACGSMVHAALTAADLLAQEGIACSVTDMHTL
                     KPLDTAHLDHLAGSARLLVTIEEHSRIGGLGSAVAEHLCAMDAHPPLLPIALPDAFGK
                     SGEYAFLLDHYGLTPGKLADRIGERYRRLVPAA"
     gene            complement(2443..3372)
                     /locus_tag="PLN02_02830"
     CDS             complement(2443..3372)
                     /locus_tag="PLN02_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51283.1"
                     /translation="MNNNGYILCRPQGGLNDMLCQIEHCCRYAEKFSRTVIVDTKYEK
                     AIHFRDRISNYFVSQQQGLILDATEADIEAANNSIFPYCVMGKVRDYDFHFSWDKRLY
                     VESSTLTPLTFETHKHYPTKYLLHHTGGSGQHSICALQRMRLHDSVVDLLIERLEAIG
                     RDYVGIHIRNTDYETDYHNSLHELKTFENTPILVCTDSQAVLDDARAIIGEDRVISFS
                     NLETGEDKRIHFFTDDSLARQRNLDAIVDLLLLAFSTYLVTLPLKINKEAPQGGYSGF
                     SVLAGLLHNNRTTLMSIIGRQHRTLRKFAFSLI"
     gene            complement(3429..4247)
                     /locus_tag="PLN02_02835"
     CDS             complement(3429..4247)
                     /locus_tag="PLN02_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOI51284.1"
                     /translation="MNFDELAEMARFMRRKILDISHACNLSTHLGGGLSMVDITATLY
                     GSVLRFDRNDPLWPERDRFILSKGHGVLGYYSALLAAGIISEETFASFQTNGSDLIAH
                     PVMNPALGIESSNGSLGHGLSLAIGIALAARKKGQAHKTYVLLGDGECNEGAIWEGVM
                     LAAQLKLANLVAIVDYNGLQSDGESSQIIDLGDLAAKFAAFGWNAVTVDGHDIPALHA
                     ALTAPSPDRPRAVIARTVKGKGISFMENNNEWHHNRLTKASYDSAVEELSAPCR"
     gene            complement(4261..>4809)
                     /locus_tag="PLN02_02840"
     CDS             complement(4261..>4809)
                     /locus_tag="PLN02_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI51285.1"
                     /translation="TSADATKEAVTRIVGLRRRIDILVNNAGAASGALFQMTTQRDLR
                     QIFEINFFAQLQFTQSISRLMTRQRSGSIINLASIAGLIGEPGMTAYGASKAALIQAT
                     RTLASELGGAGIRVNAIAPSITHTDMYEQMDPAARERLIAASALKRAARPEEIANVAL
                     FLASDLSSYITGQVIRADGGIL"
BASE COUNT          777 a         1366 c         1755 g          911 t
ORIGIN      
        1 ccagcttgcc ctggcgggag gcgagctcga tcagcttggc gaggatttcc ggctgcttgc
       61 tggtcgccga agccgcgctc agcatccaca gcgcgtcctg cgcctgaccg ttgcggtaga
      121 gggcttcggc cagttcggga taggtcgacg gatcggaggg atcgcgttgc tggtattcct
      181 ggaagtagcg gatggcgtcg cgcgggttgc cgcaacgcag caggtggcgc gccagttcgg
      241 cgagatagtc ggcgcgttgc ggctccagca gcatcgcctc ggcgacgaag gaagcgctgt
      301 gctcgaacat gccgagatcg gcgctgacca gcgcgagatg gaagaaggcc tcgccgtcgt
      361 ccggaaagtt ggaaacgtag cgcgacagca gcagcaaggc gccctcgagg ttgccggcct
      421 ggcgctgggc gatgccggca ttcaggtcgt tgcgcggcag gccggcgatg ttgcggctgc
      481 tcttgctgta acagctttcc atgctcgccg ccgcggcggc attctgggtg agctcgacga
      541 aggcttcgaa ctggggttcg aatcgctggt agatggccag tgcctggcga tggatttcgt
      601 gcaccgtcgc cttggcgcgc cggatttcgt cttcgctgtc gccccaggca atcccgtcat
      661 tgctgaaatc gctgaccggc atcgccggaa gccagcgctc gttggggaga tagacgatcg
      721 ggcagccgca catccgggct tcgacggtga tcgctccctc ttcgtagcag tacagcagtt
      781 cggcggtctt gaagatgtcg ctgagttcct gcaggctgcg cggctgccgc ggcgagattt
      841 cgatggcgcc gtcggtgacg ggcagcagtt cgccgcccga actgagcagg cggttgaaga
      901 agaaatacct gcccgaacgc tctgcctcgg gaagccccgg tccgaacagg cgggggtcgg
      961 aggccgggac ggtcagcaac ggaatctcgg ggtgatcgac aaatgcctcg cgatagccga
     1021 tcgtgatttc gccttcggcg aagtcggcat ggccggatat cttgcccggc ggcgccagca
     1081 gccagcgcac cggaacgccg acgccgagcg gggtgccgcg ggtgatttcg gggtagatga
     1141 cgatcggctt gagatcgagc gcgaaatggc gattgatgac ggcttcggtc aggcacgggg
     1201 tccacagttc gggcgaagtc ttaggcgtga cgacataggc ttcgtaaccg tgcaggttca
     1261 ggatgtgaca caggtagtgc atcacccgga tgccgcagga aagttcggtg aagtccggcg
     1321 cgtacaggta ataggggtgc cggtactgcc ggtggtgttc tttgatcgag atctgcattg
     1381 cggcctgggc ggtaggggag agggcggggg cgggaggtgt cggttcaggc ggccgggaca
     1441 aggcgtcggt agcgttcgcc gatccggtcc gccagtttgc cgggcgtcag cccgtagtgg
     1501 tcgagcagga atgcgtactc cccgctcttg ccgaatgcgt cgggcagcgc gatcggcaac
     1561 agcggcggat gcgcgtccat ggcgcagaga tgctcggcga cggcgctccc cagcccgccg
     1621 atccggctgt gttcctcgat cgtcacgagc agccgggcgc tgccggcgag atggtcgagg
     1681 tgcgcggtgt ccagcggctt cagggtgtgc atgtcggtga cgctgcaggc gatgccttcc
     1741 tgcgccagga ggtcggcggc ggttagcgct gcgtgcacca tcgaaccgca ggcgacgagc
     1801 gcgacgtcgc cgccttcgcg caaggggatc gcccgtccga gctggaacgg aaaatcctcg
     1861 cgatagacga tcggcgtgtt gggggcgccg gtcagccgga tgtacatcgg cccggcatgg
     1921 ctggccgcgg cgaacacggt cttggcgatg gcgccgcaat cggcggggct gaccacggtc
     1981 aggttgggga tgctgcgcat gatggcggcg tcttccagcc cgtagtgcga gttgcccaga
     2041 aagcccatgc ccagtccgct gccgatgccg accgccttga cgttgaagcc catgtagccg
     2101 aggttcatgc ggatctgctc ggcggcgcgc atggtgatga agggggcgaa cgaggtggcg
     2161 aaaacggtga agccttcctt ggccaggccg ccggcgacgc cgatcaggtt ctgctcggcg
     2221 atgccgacgt tgaggtagcg ctcgggatag gtctgcttga agcggtcgag gccggacgaa
     2281 ttgccgaggt cggcggaaag gaccagcagt tcggggcagc tctcggccag cgccaggatg
     2341 gcctgcccga acatgcctcg cggtcccagt ctcgaccaga ggcgaacatt tccagtagaa
     2401 aactccatcg gcgatctttc ggcgtaaggg ttgggcgcgg gctcagatca ggctgaacgc
     2461 gaacttccgc agtgtacggt gctggcggcc gatgatcgac atcagcgttg tccggttgtt
     2521 gtgcagcagc ccggccagca ccgaaaaacc ggagtagccg ccttgcggcg cttccttgtt
     2581 gatcttcagc ggcagcgtca ccaggtaggt ggagaaggcc agcagcagca ggtcgacgat
     2641 ggcgtccagg ttgcgctggc gggcgaggct gtcgtcggtg aagaaatgga tgcgcttgtc
     2701 ctcgccggtc tccaggttcg agaacgagat gacgcggtcc tcgccgatga tcgcgcgggc
     2761 gtcgtcgagc accgcctggg agtcggtgca caccaggatc ggcgtgttct cgaaggtctt
     2821 cagttcgtgc aggctattgt ggtagtcggt ctcgtaatcg gtgttgcgga tgtggatgcc
     2881 gacgtaatcg cggccgatcg cttccagacg ctcgatcagc aggtcgacca cgctgtcgtg
     2941 caggcgcatg cgctgcaggg cgcagatcga atgctggccg ctgccgccgg tgtggtgcag
     3001 caggtatttg gtcgggtagt gcttgtgcgt ttcgaaggtc agcggggtca gggtggaaga
     3061 ttcgacgtag agccgcttgt cccacgagaa atggaaatcg tagtcgcgca ccttgcccat
     3121 gacgcagtag gggaagatcg aattgttggc cgcctcgatg tcggcttccg tcgcgtccag
     3181 gatcagcccc tgctgctggc tgacgaaata attggaaatg cggtcgcgga aatggatggc
     3241 cttctcgtat ttggtatcga cgatgacggt gcggctgaat ttctcggcgt agcggcagca
     3301 gtgctcgatc tggcacagca tgtcattgag gccgccttgc gggcggcaga ggatgtatcc
     3361 gttgttgttc atctgggcta tggcgcgggt ggcggaagga gtgatgggag gcggcgggtt
     3421 ggccgggctt agcggcaggg cgctgacagt tcttcgacgg cgctatcgta gctggccttg
     3481 gtcagccggt tgtggtgcca ttcgttgttg ttttccatga aggagatgcc cttgcccttg
     3541 accgtgcggg cgatcaccgc gcgcggccgg tccggcgagg gggcggtgag cgccgcgtgc
     3601 agcgccggga tatcgtggcc gtcaaccgtg acggcgttcc agccgaaggc ggcgaacttt
     3661 gccgcgaggt cgccgagatc gatgatctga ctgctttcgc catcgctctg cagaccgttg
     3721 tagtcgacga tcgccaccag attggctagc ttgagctggg cggcgagcat cacgccctcc
     3781 cagatcgcgc cctcgttgca ttcgccgtcg ccgagcagca cgtaggtctt gtgtgcctgg
     3841 cctttcttgc gggcggcgag cgcgatgccg atcgccagcg agaggccgtg cccgaggctg
     3901 ccgttcgacg actcgatgcc gagcgccggg ttcatcaccg ggtgggcaat caggtcgctg
     3961 ccgttggtct ggaaggaagc gaaggtttcc tcggaaatta ttccggctgc cagcagtgcg
     4021 ctgtagtagc cgaggacgcc gtgccccttg ctcaggatga agcggtcgcg ttccggccac
     4081 agcggatcgt tgcggtcgaa acgcagcacg ctgccgtaga gggtggcggt gatgtcgacc
     4141 atcgacaggc cgccgccgag gtgggtactc agattgcagg cgtggctgat gtcgaggatt
     4201 ttgcgccgca tgaagcgcgc catttctgcc agttcgtcga aattcatcgc gggggtgctc
     4261 ctacagaatg ccgccgtcgg cgcggatgac ctggccggta atgtagctgg aaaggtcgct
     4321 ggccaggaac agggcgacgt tggcaatctc ttccggccgg gcggcacgct tcagcgccga
     4381 ggcggcgatc agccgttcgc gggcggccgg gtccatctgc tcgtacatgt cggtatgggt
     4441 gatgctgggg gcgatggcat tgacccggat gccggcgccg ccgagttcgc tggccagggt
     4501 gcgggtggcc tggatcagcg ccgccttgct ggcgccgtag gcggtcatcc ccggttcgcc
     4561 gatgaggccg gcgatcgagg ccaggttgat gatgctgccc gaacgctggc gcgtcatcag
     4621 gcgcgagatc gactgcgtga actgcaactg ggcaaagaaa ttgatttcga agatctgccg
     4681 caggtcccgc tgcgtggtca tctggaacag cgcgccgctg gccgcgccgg cgttgttgac
     4741 caggatgtcg atccggcgcc gcaggccgac gatgcgggtg acggcttcct tggtcgcgtc
     4801 ggccgaggt
//
LOCUS       DAOSDX010000085         4775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6308_length_4775_cov_3.548729, whole genome shotgun sequence.
ACCESSION   DAOSDX010000085 DAOSDX010000000
VERSION     DAOSDX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6308_length_4775_cov_3.548729"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /gene="lptD"
                     /locus_tag="PLN02_02845"
     CDS             complement(<1..2044)
                     /gene="lptD"
                     /locus_tag="PLN02_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOI51286.1"
                     /translation="MTGFSRRPIAALVCCLFAGVPFAQANTGEAESAAAAIGAAPLRL
                     EKRFNLLATRKTAPKLAAAGGLVPLSSVDDPLPLFLTADHLEGQASELAVAEGNVELR
                     KSGLELRADRLTYKPLDDEVDASGKVVLLQDGAEIETTHLRMKLSDQLGFAEESRYSV
                     TKEVASRVYETNQNVVTVASVSGANSGAPMMLNVANVYGLPTEQAAPRQITAQGSAER
                     IEFAGENRMRLDNSTFSTCKPASPDWYLKAGEIELDYNENYGNASDASLWFKEVPLFY
                     APKAWFPMNSQRRSGLLTPSFKQSTKTGLDISLPIYWNIAPNYDATFYPRYMSKRGTQ
                     LGLETRYLSERSEGEWRGEYLGDDASDHTRRYAYSIRHLQRFTDDLTGALNWNGVSDD
                     TYWEDMSSRLLQTSQVQLERRASLNYSPSPWWQGSLQVLRYQTLQPDPANPVARPYFL
                     EPQLNLLGYKPDLLGFDFTMLGQYSRFTHDDSANKDRGDRFVLYPQLSYPVIGAAYQI
                     TPKLGLHMSSYAIDRATLNGGGSDNITRVLPTFSLDATTVFERDLDLLGHRFLQTLEP
                     RLYYVYIPYKDQSEIPVFDTALSDFNFAQVFSENRYSGYDRINDAHQLTAALTTRILD
                     GTTGAERFKAMIGQRYYFAKQRVTLPGETSRPDNFSNLIASVTGLVAPKTYAEATWE"
     gene            2132..3145
                     /locus_tag="PLN02_02850"
     CDS             2132..3145
                     /locus_tag="PLN02_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOI51287.1"
                     /translation="MSRDQLVTDWVASRFPGQSVSITPASADASFRRYFRLTWPDGST
                     RILMDAPPEKEDCKPFIKVAGLLAKADLAAPRILDQDLENGFLVLTDLGRIGYLDALN
                     ADLSLADLLIRPVLDVLVKWQLSSTAATLPPYDAPLLRRELDLFPEWFIGRHLGIELD
                     ANDKLMLDRTFKFLINSALNQPKVFVHRDFMPRNLMVVESAETLTPGVIDFQDAVYGP
                     ITYDVVSLFRDAFISWEEEQEIDWVVRYWEKARAAGLPVRTDFGAFWRDYELMGLQRH
                     LKVLGIFCRLNYRDGKDKYIADLPRFMTYAKKTASRYLQLKPLLNLLDRLDGQTEQIG
                     YRR"
     gene            3152..3838
                     /locus_tag="PLN02_02855"
     CDS             3152..3838
                     /locus_tag="PLN02_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOI51288.1"
                     /translation="MKAMILAAGRGERMRPLTDHTPKPLLEAGGKPLIVWHLEKLAAA
                     GFREVVINHAHLGEKIENALGDGSQWGLSIAYSPEPPGALETAGGIARALPRLGNAPF
                     LVVNGDVYCDVAFPAFAARGYPPGGARLLLVDNPAHHGGGDFSLSGERIVPADGERTL
                     TYAGIGVFSPAMFDHVPADQPMKLRPLLDAAIASGTLTGERHAGRWVDVGTPQRLAEL
                     DTELRAAQAD"
     gene            3882..4733
                     /gene="dinD"
                     /locus_tag="PLN02_02860"
     CDS             3882..4733
                     /gene="dinD"
                     /locus_tag="PLN02_02860"
                     /inference="COORDINATES: protein motif:HMM:NF008573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HOI51289.1"
                     /translation="MTDSPVSRQHHASFEGLRQLDDAGNEYWLARKLATALDYSQYRH
                     FLPAIERARIACEQSGQAPSDHFEDVLTMVDIGSGAKRQIEDIRLSRYACYLIVQNGD
                     PSKPVIANGQTYFAIQTRRQELANDTAFGQLSEDEKRLAIRNELAQHNKYLAAAAAEA
                     GVESGLDFAVFQDHGYRGLYGGLGAKAIHARKGLKKSQKILDHMGSTELAANLFRATQ
                     TEEKLRRDGVKGKQEANQTHLAVGRKVRETIRELGGTMPEDLPTPERSIQQIETAQQR
                     ITKKDND"
BASE COUNT          906 a         1584 c         1476 g          809 t
ORIGIN      
        1 attcccaggt cgcctcggca taggtcttcg gcgcgacgag gccggtgacc gaggcgatca
       61 ggttggagaa attgtccggc cgcgaggtct cgccgggcag ggtgacgcgc tgcttggcaa
      121 agtagtagcg ctgaccgatc atcgccttga agcgctcggc accggttgtg ccgtcgagga
      181 tgcgggtggt cagtgccgct gtcagctggt gggcgtcgtt gatccggtcg tagccgctgt
      241 aacggttttc ggagaagacc tgggcgaagt tgaaatcgga cagcgcggtg tcgaagaccg
      301 gaatctcgct ctggtccttg tacgggatgt agacgtagta gagccgcggc tccagcgtct
      361 gcaggaaacg gtgaccgagg aggtcgaggt cgcgctcgaa gacggtggtg gcatcgagcg
      421 agaaggtcgg caacacccgc gtgatattgt cggagccgcc gccgttgagc gtcgcccggt
      481 cgatcgcgta gctgctcatg tgcaggccga gcttgggcgt gatctggtag gcggcgccga
      541 tcaccgggta gctcagttgc ggatagagga caaaacggtc gccgcggtcc ttgttggcgc
      601 tgtcatcgtg ggtgaagcgc gaatactggc cgagcatcgt gaagtcgaaa cccagcaggt
      661 ccggcttgta gccgagcagg ttcaactgcg gctcgaggaa gtagggacgg gccaccggat
      721 tggcagggtc aggctgcagc gtctggtagc gcagcacctg cagcgagccc tgccaccacg
      781 gactgggcga gtaattgagc gaggcccgcc gttcgagttg tacctgcgag gtctgcagca
      841 ggcgcgacga catgtcttcc cagtaggtgt cgtcggaaac gccgttccag ttcagggcgc
      901 cggtgaggtc gtcggtgaaa cgctgcaggt ggcggatcga gtaagcgtaa cgacgggtgt
      961 gatcgctcgc gtcgtcgccc aggtattcgc cgcgccactc gccctcgctg cgctcgctca
     1021 ggtaacgcgt ttccaggcca agctgggtgc cgcgcttgct catgtagcgc ggatagaagg
     1081 tggcgtcgta gttgggcgcg atgttccagt agatgggcag actgatgtcg agaccggtct
     1141 tcgtcgactg cttgaacgac ggcgtcagca ggccggaacg ccgctggctg ttcatcggga
     1201 accaggcctt cggcgcgtag aacagcggca cctccttgaa ccacagcgag gcatcgctgg
     1261 cgttgccgta gttctcgttg taatcgagtt caatctcgcc ggccttcagg taccagtccg
     1321 gtgacgccgg cttgcaggtg gaaaaggtgc tgttgtcgag gcgcatgcgg ttctcgccgg
     1381 cgaactcgat acgttcggcg ctgccctgcg cagtgatctg gcgcggcgct gcctgctcgg
     1441 tcggcagacc atagacgttg gcgacattga gcatcatcgg cgcgccactg ttggcgccgc
     1501 tgacactggc cacggtgacg acgttctggt tggtctcgta gacgcggctg gcgacttcct
     1561 tggtcaccga ataccgcgat tcctcggcga aacccagctg gtccgacaac ttcatgcgca
     1621 ggtgcgtcgt ctcgatctcg gcaccatcct gcagcagcac aaccttgccg ctggcatcga
     1681 cctcgtcgtc gagcggcttg taggtcaggc ggtcggcacg cagttccagg cccgacttgc
     1741 gcagttcgac attgccttcg gcaacagcca gttcagaagc ttgaccttcc agatgatcgg
     1801 cagtgaggaa gagcggcagc ggatcgtcaa cgctggacag gggcaccaga ccgccggcag
     1861 cggccagctt cggagccgtc ttgcgcgtcg ccagcaggtt gaaacgcttc tccagacgca
     1921 gcggcgcggc accgatggct gcggcagcgc tttcggcctc gcccgtgttc gcctgggcga
     1981 acggaacgcc ggcaaacagg caacagacca gcgcggcaat cggacgtcgg gaaaaaccag
     2041 tcatcggggc attctggagg tgccgggacc ggaaaggccg atgcccgggt gttaaaatcg
     2101 cgccatttta caacgaacat tcccctactc gatgtcgcgc gatcaacttg ttaccgactg
     2161 ggttgccagt cgcttccccg ggcagtccgt ctccatcacg ccggcttccg ccgacgccag
     2221 cttccgccgc tatttccgcc tgacctggcc ggacggcagc acccgcatcc tgatggacgc
     2281 gccaccggaa aaagaagact gcaagccctt catcaaggtc gccggcctgc tcgccaaggc
     2341 cgacctggcg gcaccgcgca ttctcgacca ggacctcgag aacggcttcc tggtgctcac
     2401 cgacctcggc cgcatcggct acctcgacgc cctcaacgcc gatctctcgc tggccgacct
     2461 gctgatccgc ccggtgctcg acgtgctggt caaatggcag ttgtcgtcca cagccgccac
     2521 cctgccgccc tacgacgcgc cgctgctgcg ccgcgaactc gacctcttcc cggaatggtt
     2581 catcggccgc cacctcggca tcgaactcga cgccaacgac aaactgatgc tcgaccggac
     2641 cttcaagttc ctgatcaact cggcgctcaa ccagcccaag gtcttcgtcc atcgcgactt
     2701 catgccgcgc aacctgatgg tcgtcgaaag cgccgagacg ctgaccccag gcgtcatcga
     2761 cttccaggac gcggtctatg ggccgatcac ctacgacgtc gtctcgctgt tccgcgacgc
     2821 cttcatttcc tgggaggaag agcaggaaat cgactgggtc gtccgctact gggaaaaggc
     2881 ccgcgccgcc ggcctgccgg tgcgcactga cttcggcgct ttctggcgcg actacgaact
     2941 gatgggcctg cagcgccacc tcaaggtgct cggcatcttc tgccgcctca actatcgcga
     3001 cggcaaggac aagtacatcg ccgacctgcc gcgcttcatg acctacgcca agaagaccgc
     3061 cagccgctac ctgcaactga aaccgctgct caacctgctc gaccgcctcg acggccagac
     3121 cgagcagatc ggctaccgcc gctaaacgcc gatgaaagcg atgatcctcg ccgccggccg
     3181 cggcgaacgc atgcggccgc tgaccgacca cacgccgaaa ccgctgcttg aagccggcgg
     3241 caagccgttg atcgtctggc acctggaaaa gctggccgcc gccggtttcc gcgaagtggt
     3301 catcaaccac gcccacctcg gcgaaaagat cgaaaacgcg ctgggcgacg gatcgcagtg
     3361 gggactcagt atcgcctact ccccggaacc gccgggcgca ctggaaaccg ccggcggcat
     3421 cgcccgggcc ttgccgcggc tcggcaacgc ccccttcctg gtggtcaatg gcgacgtcta
     3481 ctgcgacgtc gcttttcccg ccttcgccgc gcgcggctac ccgcccggcg gcgcccgcct
     3541 gctgctggtc gacaacccgg cgcaccacgg cggcggcgac ttctcgctga gcggcgaacg
     3601 gatcgtcccg gccgatggcg agcgaacgct cacctacgcc ggcatcggcg tcttttcgcc
     3661 ggccatgttc gaccatgtgc cggccgacca gccgatgaag ctgcgcccgc tgctcgacgc
     3721 agcaattgcc tccggcacgc tgaccgggga acgccacgcc ggccgttggg tcgacgtcgg
     3781 cacaccacaa cggctggccg agctggatac cgaattgcgc gcagcacagg cagattgaat
     3841 tccaatagca tggcgctttc agcccctgga ggaatgccgc gatgaccgac tcacccgttt
     3901 cccgccagca ccacgccagc tttgaaggcc tgcgccaact cgacgacgcc ggcaacgaat
     3961 actggctggc ccgcaaactg gcaacagcac tggattattc gcaataccga cactttcttc
     4021 ccgccatcga acgcgccagg attgcctgtg aacaaagtgg ccaagctcct tccgaccatt
     4081 ttgaggatgt cctcacaatg gtcgacatcg gctcgggggc aaaacggcag atcgaagaca
     4141 tccgcctctc ccgctatgcc tgctacctga tcgtccagaa cggcgacccc tcgaagccgg
     4201 tcattgccaa cggccagact tactttgcca tccagacccg ccgccaggaa ctggcgaacg
     4261 acaccgcctt cgggcaactg agcgaagacg agaagcggct cgccatccgc aacgaactcg
     4321 cacaacacaa caaatacctt gctgccgctg ccgccgaggc cggcgtcgaa agtggcctcg
     4381 acttcgccgt tttccaggac catggctatc gcggcctgta cggcgggctc ggtgccaagg
     4441 ccatccacgc tcgaaaaggc ttgaagaaaa gccagaaaat cctcgaccac atgggcagca
     4501 ccgagctggc cgccaacctg ttccgcgcca cgcaaaccga ggagaaattg cgccgcgacg
     4561 gggtcaaagg caagcaggag gcgaaccaga cccacctcgc cgtcggccga aaagtgcgcg
     4621 agacgatcag ggaactcggc ggtaccatgc cggaagacct gccgacgccc gagcgcagca
     4681 tccagcaaat cgagaccgcg cagcaaagaa tcaccaagaa agacaacgac tgaatgactc
     4741 acgcccactt catcgcccgc cgccggcgcc tgctg
//
LOCUS       DAOSDX010000086         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6311_length_4773_cov_3.158966, whole genome shotgun sequence.
ACCESSION   DAOSDX010000086 DAOSDX010000000
VERSION     DAOSDX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6311_length_4773_cov_3.158966"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1972)
                     /locus_tag="PLN02_02865"
     CDS             complement(128..1972)
                     /locus_tag="PLN02_02865"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOI51290.1"
                     /translation="MTVALNAPEYVNHEALKKWVAEIAELTQPDQIHWCDGSQEEYDR
                     LCAEMVAAGTLIKLNPEKRPNSYLAFSDPSDVARVEDRTYICSEKKEDAGPTNNWEEP
                     AKMRETLNGLFKGCMKGRTMYVIPFSMGPLGSPIAHIGFEITDSAYVVTNMKTMTRMG
                     KAVFDVLGSDGEFVPCIHTVGAPLEAGQKDVAWPCNPTVKYIVHYPETREIWSYGSGY
                     GGNALLGKKCFALRIASNMAREQGWLAEHMLILGVESPEGEKTYVAAAFPSACGKTNF
                     AMLIPPKTLGGWKITTIGDDIAWIKPRVDADGVTRFYAINPEAGFFGVAPGTSEKTNY
                     NALATLKENIIFTNVALTDDGDVWWEGMTKEAPAHLIDWQGKDWTPEDGKNGRKAAHA
                     NSRFTAPANQCPSVDPAWEDPAGVPISAFIFGGRRATTVPLVYQAFNWNYGVYMAATL
                     GSETTAAAFGQQGVVRRDPFAMLPFCGYHMGDYFNHWLRMGKAVDATPKIFCVNWFRM
                     DKDGNFMWPGFGDNMRVLKWIVDRCKGKVAAKETTLGWMPKFEDIDWTGLDIKPEDFE
                     ALTTIDADAWKAELAGHKEWFDKMGEKMPRELVLKRELFEMGIFKDGV"
     gene            2218..4497
                     /locus_tag="PLN02_02870"
     CDS             2218..4497
                     /locus_tag="PLN02_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOI51291.1"
                     /translation="MDPRQLREAALFYHRSPKPGKIAVTPIKQLTNQYDLSMAYSPGV
                     AFACEEIVADPAEASTLTARANLVGVITNGTAVLGLGPIGPLAAKPVMEGKGVLFKKF
                     ANIDVFDLEIDERDPDKLIETIASLEPTFGGINLEDIKAPECFYIEKKLRERMKIPVF
                     HDDQHGTAIVVGAAILNGLHLIGKDLSKVKIVTSGAGAAALACLDLLVMLGAPVENIW
                     VTDIKGLVYEGRVEEMDEIKARYAKKTEQRTLGEAIADADVFLGLSAGGVLKPEMVAK
                     MAARPLIMALANPTPEITPELVKSVRDDAIMCTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATTITEEMKMAAVKAIAELARAEQSEVVASAYGERISGFGPEYLIPKPFDPRLIVKI
                     APAVAQAGMDSGVATRPIQDWDAYLQGLNEFVYHSGLIMKPVFSQARLAPKRIVFAEG
                     EDERVLRAVQVVRDEGIAWPILIGRHAEIKRRIEAAGLRIREGEDFEVIHSDNSAFFD
                     EHYEEFWTTYHRIMERKGVSPDYARREVRRRNTLYGALMVRLGYADGLICGTFGRHET
                     HREYVQSVIGTRAEHDRYYAMNLLLLPGRTVFIADTYVNYDPSAEQLVDMTLMAAEEI
                     RNFGIVPKVALLSHSTFGTENTPTAIKMREVLAELNRRAPELEVEGEMHGDAALDEKI
                     RQTAFPNSRLKGQANLLVMPTLDAANISFNLLKVAAGDNLTVGPILLGAAKPVNILTP
                     TATVRRIVNMTALTVVDAS"
BASE COUNT          877 a         1423 c         1539 g          934 t
ORIGIN      
        1 acgacgtgga agggcgcgat gtcggcaaga cgggcggcag aaccgggcat tgcatactcc
       61 agaaagacga cggccaccga aatgatcggt ggccgtggcg gactcagctc attccgttat
      121 ccggatatca gacgccgtcc ttgaagatgc ccatttcgaa cagttcgcgc ttcaacacca
      181 gttcgcgcgg catcttctcg cccatcttgt cgaaccactc cttgtggccg gccagttcgg
      241 ccttccaggc gtcggcgtcg atcgtcgtca gggcttcgaa gtcttcgggc ttgatgtcga
      301 ggccggtcca gtcgatgtcc tcgaacttcg gcatccagcc gagcgtggtt tccttggcgg
      361 caaccttgcc cttgcagcgg tcgacgatcc acttcaggac gcgcatgttg tcgccgaagc
      421 ccggccacat gaagttgccg tccttgtcca tgcggaacca gttgacgcag aagatcttcg
      481 gcgttgcatc gacggccttg cccatgcgca gccagtggtt gaagtagtcg cccatgtggt
      541 agccgcagaa gggcagcatg gcgaacgggt cgcggcgcac cacgccctgc tggccgaagg
      601 cggcagcggt ggtttcggag ccgagggtgg cggccatgta gacgccgtag ttccagttga
      661 aggcctggta gaccagcggc acggtggtgg cgcgacggcc gccgaagatg aaggccgaga
      721 tcggcacgcc ggcgggatct tcccaggccg ggtcaaccga cgggcactgg ttggccgggg
      781 cggtgaagcg cgagttggcg tgcgccgcct tgcgaccgtt cttgccgtct tccggcgtcc
      841 agtccttgcc ttgccagtcg atcaggtggg ccggggcttc cttggtcatg ccttcccacc
      901 agacgtcacc gtcgtcggtc agcgcgacgt tggtgaagat gatgttttcc ttcagcgtgg
      961 ccaaggcgtt gtagttggtc ttttccgagg tgcccggggc gacgccgaag aagccggctt
     1021 ccgggttgat ggcgtagaag cgggtgacgc cgtcggcatc gacgcgcggc ttgatccagg
     1081 cgatgtcgtc gccgatggtg gtgatcttcc agccgcccag ggtcttcggc gggatcagca
     1141 tggcgaagtt ggtcttgccg caggccgacg ggaaggcggc ggcgacgtag gtcttttccc
     1201 cttccggcga ttcgacgccg aggatgagca tgtgttcggc cagccagcct tgttcgcgcg
     1261 ccatgttgga agcgatgcgc agcgcgaagc acttcttgcc gagcagggcg ttgccgccgt
     1321 aaccggaacc gtaggaccag atttcgcggg tttccgggta atgcacgatg tacttgacgg
     1381 tcgggttgca cggccaggcg acatccttct ggccagcttc gagcggggcg ccgacggtgt
     1441 ggatgcaggg gacgaattcg ccatcgctgc cgagcacgtc aaagacggcc ttgcccatgc
     1501 gggtcatcgt cttcatgttg gtgacgacgt aggcggagtc ggtgatttcg aaaccgatgt
     1561 gggcgatcgg cgaaccgagc gggcccatcg agaaggggat gacgtacatc gtgcggccct
     1621 tcatgcagcc cttgaacagg ccgttcaggg tttcgcgcat cttggccggt tcttcccagt
     1681 tgttggtcgg gccggcgtct tccttctttt cggagcagat gtaggtgcgg tcttcgacgc
     1741 gggcgacgtc ggaggggtcg gagaaggcga ggtaggagtt cgggcgcttt tccggattga
     1801 gcttgatcag cgtgccggct gcaaccattt ccgcgcacag acggtcgtat tcttcctgcg
     1861 agccgtcaca ccagtggatc tggtcgggtt gagtcagttc ggcaatctcg gcgacccact
     1921 tcttgagggc ttcgtggttg acgtactctg gtgcgttcag cgctacggtc atggcggtgt
     1981 ctctctaata agttactgaa ataactgatt tttcacgcca gtcgccgggt gctgccacca
     2041 agacatctgc gcggcgaggg gccgtcggct gggaagcccg taattatgcc tcaggcaggg
     2101 ggaaatatct acttgacttt gcctgctaag tgatgtccga aatatcgctg tcaaatgttg
     2161 tgtgctatag ttttccgcat ctcaaaaaca attttcataa tataaaacgg aggcgacatg
     2221 gatcccaggc aattgcgcga agccgcgctg ttctatcacc gtagtccgaa acccggcaag
     2281 atcgcggtca cgccgatcaa gcagctcacc aaccagtacg acctgtcgat ggcttattcg
     2341 ccgggcgttg ccttcgcctg cgaggaaatc gtcgccgatc cggccgaggc gagcacgctg
     2401 accgcccgcg ccaacctggt cggtgtcatc accaacggca ccgccgtcct cggtcttggg
     2461 ccgatcggcc cgctcgccgc caagccggtg atggaaggca agggcgtgtt gttcaagaag
     2521 ttcgccaaca tcgacgtctt cgacctcgag atcgacgagc gcgatccgga taagctgatc
     2581 gagacgatcg cctcgctgga gccgactttc ggcggcatca acctcgaaga catcaaggcg
     2641 cccgagtgct tctacatcga gaagaagctg cgcgagcgga tgaagatccc ggtcttccac
     2701 gatgaccagc acggcacggc gattgtcgtc ggcgccgcca tcctcaacgg tctgcacctg
     2761 atcggcaagg atctgtccaa ggtcaagatc gtcacttccg gtgctggtgc cgccgcgctc
     2821 gcctgtctcg acctgctggt catgctcggc gcgccggtgg agaacatctg ggtcaccgac
     2881 atcaagggcc tggtctatga aggccgggtc gaggaaatgg acgagatcaa ggcgcgctac
     2941 gccaagaaga ccgagcagcg cacgctcggc gaggcgattg ccgatgccga cgttttcctg
     3001 gggctctcgg ccggcggcgt actgaagccg gaaatggtcg ccaagatggc cgccaggccg
     3061 ctgatcatgg cgctcgccaa cccgacgccg gaaatcacgc ccgagctggt caagtcggta
     3121 cgcgacgacg ccatcatgtg cactggccgt tcggattacc cgaaccaggt caataacgtg
     3181 ctctgctttc ctttcatttt ccgtggcgcg ctcgacgtcg gggcgacgac gattaccgag
     3241 gaaatgaaaa tggcggcggt gaaagcgatc gccgagctgg cccgcgccga gcagtcggaa
     3301 gtcgtcgctt ccgcctatgg cgagcggatt tccggctttg gtccggaata cctgattccg
     3361 aaacccttcg atccgcggct gatcgtcaag attgcgccgg cagtggcgca ggcaggcatg
     3421 gattcgggcg tcgcgacgcg gccgattcag gactgggacg cctatctgca gggcttgaac
     3481 gaattcgtct atcactccgg cctgatcatg aagccggtgt tctcgcaggc caggctggcg
     3541 cccaagcgca tcgtcttcgc cgagggcgag gacgagcgcg tgttgcgcgc tgtgcaggtg
     3601 gtgcgcgacg agggcatcgc ctggccgatc ctgatcggtc gtcatgccga gatcaagcgc
     3661 cggatcgagg cggccggcct gcgcatccgc gaaggcgagg acttcgaggt catccactcc
     3721 gacaacagcg ccttcttcga cgaacattac gaggaattct ggaccaccta ccaccggatc
     3781 atggagcgca agggcgtgtc gcccgattac gcgcgccgcg aggtgcgccg ccgcaacacg
     3841 ctgtacggtg cgctgatggt ccgtctcggc tacgccgacg gcctgatctg cggtaccttc
     3901 ggtcgccatg agacgcatcg tgaatacgtg cagagcgtca tcggcacccg tgccgagcat
     3961 gaccgctact acgcgatgaa cctgctgctg ctgccggggc gcaccgtttt tatcgccgac
     4021 acctacgtaa actacgaccc gagcgccgag caactggtcg acatgacgct gatggcggcc
     4081 gaggaaatcc gcaacttcgg catcgtgccg aaggtcgcgc tgctctcgca ctccactttc
     4141 gggaccgaga acacgccgac ggcgatcaag atgcgcgagg tgctggccga actcaaccgg
     4201 cgcgccccgg agctcgaggt cgaaggcgaa atgcacggcg acgcggcgct cgacgagaag
     4261 atccgccaga ccgccttccc gaattcgcgg ctcaaggggc aggccaacct gttggtcatg
     4321 ccgaccctgg atgcggcgaa catctcgttc aatctgctca aggtggcggc tggcgacaac
     4381 ctgacggtcg ggccgatcct gctcggcgcg gcgaagccgg tcaatatcct gacgccgacg
     4441 gcgacggtgc gccgcatcgt caacatgacg gcgctcaccg ttgtcgacgc ttcctgagta
     4501 agtgcctgaa tatccggttc tatcttgctg ctttgattgt aattttggat ttgaatcata
     4561 aactcggata gaatcggcgc ttgcttcggg agtgcctcca tgcgtcgaaa attacttgtc
     4621 ctgctcgcgt ccttgtcgct gatcgtcggc gtgtcgccgg tcgaggcagc aaaacgggaa
     4681 gcgacaacca agaagccggt gtcgcataaa tcggcgaaga aaacgcataa ggcacagaag
     4741 aagttggtgg cggcgcgcag caccaaaccg gtg
//
LOCUS       DAOSDX010000087         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6327_length_4767_cov_3.410229, whole genome shotgun sequence.
ACCESSION   DAOSDX010000087 DAOSDX010000000
VERSION     DAOSDX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6327_length_4767_cov_3.410229"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /gene="polA"
                     /locus_tag="PLN02_02875"
     CDS             <1..1644
                     /gene="polA"
                     /locus_tag="PLN02_02875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOI51292.1"
                     /translation="CYIPLAHNGPGVAEQLPRDEVLAKLKPWLENAERRKVLQNAKYD
                     QHVFANHHIALAGIAHDTMLQSYVIESDKGHDLGQLCSRHLGLDTIAYEDLCGKGAKQ
                     IGFDQVDIERAATYAAEDADVTLRVHDVLHPQFAKEPGLSRIYHEIELPARQVIWQME
                     RSGILIDGDTLARQSHEIGQKIMALEAQAYELAGQPFNLASPKQLGEILFGKLGLPVK
                     KKTASGGASTDEEVLSELALDYPLPKLLLEHRSLSKLKGTYTDKLPRMINPDSGRVHT
                     HFSQASVVTGRLASSEPNLQNIPVRTDEGRKIRTAFVAPEGSSIVSADYSQIELRIMA
                     HLSGDARLLDAFAHGEDVHRATAAEIFGVTPLEVGPDQRRVAKSINFGLIYGMSAFGL
                     ARQLGLERSAAQTYIDRYFARYPGVARHMEEARATVREHGYVETVFGRRLWLPEIRSS
                     NGNRRQAAERAAINAPMQGTAADLIKLAMIAVQNWLEQTARQSKLVLQVHDELVLEVP
                     DAELMEIRTHLPRLMTQVAQLKVPLVVEVGVGPNWEAAH"
     gene            1692..2282
                     /locus_tag="PLN02_02880"
     CDS             1692..2282
                     /locus_tag="PLN02_02880"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOI51293.1"
                     /translation="MKLADDIYVTETEYLAHEVRSPVRHEYVAGRIHAMTGASLRHNV
                     IALNIASLLRNHLRGSPCRAFMADTKLRVAKTHSIYYPDVLVTCDPQHLSVGPADQVV
                     DNPRLIVEVLSESTAATDRREKLQAYRSLPTLLEYMLVSQDEALIEIHRRSGDIGWQL
                     ATLTAGDPVELKSVELLTDFAAIYEESGLPLQPGSD"
     gene            complement(2298..2915)
                     /locus_tag="PLN02_02885"
     CDS             complement(2298..2915)
                     /locus_tag="PLN02_02885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOI51294.1"
                     /translation="MAYTRNVGLSASEDRIEKLIAERLAPGADKDKIDARIWDLFGEE
                     WAVMFTDLSGFSSGVASFGIIHFLQLIYESQRLLSPCIDAHDGILLKVEGDSLLIIFR
                     NIAKAIECADAMQRVCHDYNFGREPAERIGLCVGLGFGRVLRIGDRDVYGSEVNAAAK
                     LGEDIARAGEVLVTDAVRDAAAGVYAFEPIANVPPGANAAFRMVR"
     gene            3060..3680
                     /locus_tag="PLN02_02890"
     CDS             3060..3680
                     /locus_tag="PLN02_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HOI51295.1"
                     /translation="MFNEIIIVAAIQKIFQTRRPVATVRHTIVGAPRRAQAEAFIGDI
                     FRRHYGATVRSFAPNLMLLEDNERITAAAGWRCAGEDRLFLETYLDEPIEAAVARLAG
                     QPVKREAIVEVGNLAADRPGGSVDVILNLARHLDRLGYEWVAFTATRELIGIFRRLGL
                     PPLALAPADPERLGADAADWGSYYDSRPIVVAGRIALALEMVKRHG"
     gene            3673..>4767
                     /locus_tag="PLN02_02895"
     CDS             3673..>4767
                     /locus_tag="PLN02_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI51296.1"
                     /translation="MADFSWQAELVARPADTPLLLDSQRSWNAGEIVAELLNLRERLA
                     GSRVVGVLADNSPAWIIADLACQDAGRVHLPLPAFFHTEQLRNALEQAGADTVLTDQP
                     ERIGALDLGFAITGRWQGLTWMRRVVEPVALPPGTAKISFTSGSTGAPKGVCLSRDGL
                     LDTARAVHERLADLPLERHLAVLPLALLLENVAGVYAPLLRGMPVYLPPLAELGWAGM
                     QGFDPAALDRKVKETGAGSVILVPELLKAWATYLGFTRSQPSATLQFVAVGGARVAPE
                     LLAGARALGIPAYQGYGLTEGGSVLAINRPGDDADGVGRPLGHARLAIEDGELTVRTR
                     AFLGYTGQAATSDAAFATGDLASIDGNGHLH"
BASE COUNT          855 a         1769 c         1439 g          704 t
ORIGIN      
        1 tgctacattc cgctcgccca caacggcccc ggcgtcgccg agcagttgcc gcgcgacgaa
       61 gtgctggcaa aactgaaacc ctggctggaa aacgccgaac gccgcaaggt gctgcagaac
      121 gccaagtacg accagcacgt tttcgccaac caccacatcg cgctggccgg catcgcccac
      181 gacacgatgc tgcaaagcta cgtcatcgag tccgacaagg gccacgacct cggccagttg
      241 tgcagccgcc accttggcct cgacaccatt gcctacgaag acctgtgcgg caagggcgcc
      301 aagcagatcg gcttcgacca ggtcgacatc gagcgcgccg ccacctacgc cgccgaggac
      361 gccgacgtca cgctgcgcgt ccatgacgtg ctgcacccgc agttcgccaa ggaaccgggc
      421 ctctcccgca tctaccacga gatcgaactg cccgcccgcc aggtgatctg gcagatggag
      481 cgcagcggca tcctgatcga cggcgacacg ctggcccgtc agagccacga gatcggccag
      541 aagatcatgg cgctggaagc gcaagcctac gaactcgccg gccagccgtt caacctcgcg
      601 tcgcccaagc aactgggcga aatcctgttc ggcaagctgg gcctgccggt gaagaagaaa
      661 accgcctccg gcggcgcctc gaccgacgag gaagtgctgt ccgaactggc gctcgactac
      721 ccgctgccca agctgctgct cgaacaccgc tcgctgtcga aactcaaggg cacttacacc
      781 gacaagctgc cgcgcatgat caacccggac agcggccgcg tgcatacgca cttctcgcag
      841 gcgtccgtgg tcaccggccg gctcgcttcc agcgaaccca acctgcagaa catcccggtg
      901 cgcaccgacg aagggcggaa aatccgcacc gccttcgtcg ctcccgaagg cagcagcatc
      961 gtctcggccg actactcgca gatcgagctg cgcatcatgg cgcatctctc cggcgacgcc
     1021 cgcctgctcg acgccttcgc ccacggcgag gacgtgcacc gcgccaccgc tgccgagatt
     1081 ttcggcgtca cgccgctcga agtcggcccg gaccaacgcc gcgtcgccaa gagcatcaac
     1141 ttcggcctga tctacggcat gagcgccttc ggcctcgccc gccagctcgg cctggagcgc
     1201 agcgcggcgc agacctacat cgaccgctac ttcgcccgtt accccggcgt cgcccgccac
     1261 atggaagaag cccgggcgac ggtgcgcgag catggttacg tcgagaccgt cttcggccgc
     1321 cgcctgtggc tgccggaaat ccgttcaagc aacggcaacc gccgccaggc cgccgagcgc
     1381 gcagcgatca acgcgccgat gcagggcacc gccgccgact tgatcaagct cgcgatgatc
     1441 gccgtccaga actggctgga acagaccgcc cgccagtcga aactggtcct gcaggtgcac
     1501 gacgaactgg tgctcgaagt gccggacgcc gagctgatgg aaatccgcac ccacctgccc
     1561 cgcctgatga cccaggtggc gcagttgaag gtgccgctgg tggtcgaagt cggggtcggg
     1621 ccgaactggg aagccgccca ctaagacggt gatttattgc ataatttttc cgtcaaccgc
     1681 aggagggcgc catgaaactc gccgatgaca tctacgtgac ggaaaccgaa tacctggccc
     1741 acgaagtgcg cagcccggtg cgccacgaat acgtcgccgg ccgcatccac gcgatgaccg
     1801 gcgcctcgct gcgccacaac gtcatcgcgc tcaacatcgc gtcgctgctg cgcaatcacc
     1861 tgcgcggctc gccgtgccgc gccttcatgg ccgacaccaa gctgcgcgtc gccaagacac
     1921 acagcatcta ctaccccgac gtgctggtca cctgcgaccc gcagcacctg agcgtcggcc
     1981 ccgccgacca ggtggtggac aacccgcgcc tgatcgtcga ggtgctttcc gaaagcaccg
     2041 ccgccaccga tcgccgggaa aagctgcagg cctaccgcag cctgccgacc ctgctcgagt
     2101 acatgctcgt cagccaggac gaggcgctga tcgaaattca ccggcgcagc ggcgacatcg
     2161 gctggcagct cgccacgctg acggccggcg acccggtcga actgaagagc gtcgaactgc
     2221 tcaccgattt cgcggcgatc tacgaggaaa gcggactacc gctccagcct ggtagcgact
     2281 gacgccgccg gagcgcctca gcggaccatc cggaacgccg cattggcacc cggcggcaca
     2341 ttggcgatcg gttcgaacgc gtagacaccc gctgccgcat cgcgcacggc gtcggtcacc
     2401 agcacttcgc cagcgcgggc gatgtcttcg cccagcttgg ctgccgcgtt gacctcgctg
     2461 ccatagacat cgcggtcgcc gatccgcagc acccggccaa agccgagacc gacgcagagg
     2521 ccgatgcgtt ccgccggctc ccggccgaag ttgtagtcgt gacagacgcg ctgcatcgca
     2581 tcggcacact cgatcgcctt ggcgatattg cggaagatga tcagcaggct atcgccctcg
     2641 accttgagca ggatgccatc gtgggcatcg atgcacggcg agagcaggcg ctgcgattcg
     2701 tagatcagtt gcaggaaatg gatgatcccg aacgacgcga cgccgctgga aaagccggac
     2761 aggtcggtga acatcaccgc ccattcctcg ccgaacagat cccagatgcg ggcgtcgatc
     2821 ttgtccttgt cggcgccggg cgccagccgc tcggcgatca gtttttcgat ccggtcctcg
     2881 gatgcgctga ggccgacgtt gcgcgtgtat gccatatccc ccccccgggt acttttcagt
     2941 tggaaggaca atgatgcccc aagcggcaaa cttccggaac gcaagcgacg aatcgcgcat
     3001 ggcaaaaaag caaacgctta agtttccttt aattcgccgg tcgcatcctt ggcgccatag
     3061 tgttcaacga gatcatcatc gtggctgcca tccagaagat attccagacc cgccgaccgg
     3121 tggcgacggt ccgtcacacc atcgtcggcg cgccgcgccg ggcgcaggcg gaagccttca
     3181 tcggcgacat cttccgccgc cactacggcg ccacggtgcg cagcttcgcc cccaacctga
     3241 tgctgctcga agacaacgag cgcatcacgg ccgccgccgg ctggcgctgt gccggcgaag
     3301 accggctctt cctcgaaacc tacctcgacg aaccgatcga agccgccgtc gcccgcctcg
     3361 ccgggcagcc ggtcaagcgc gaggcgatcg tcgaagtcgg caacctcgcc gccgaccgcc
     3421 ccggcggcag cgtcgatgtc atcctcaacc tggcccgcca cctcgaccgg ctcggctacg
     3481 agtgggtcgc ctttaccgcc acccgcgagc tgatcggcat cttccgccgc ctcggcctgc
     3541 cgcctctggc cctggcgccg gccgacccgg agcgcctcgg cgccgatgcc gccgactggg
     3601 gcagctacta cgacagccgg ccgatcgtcg tcgccggccg tatcgccctg gctctggaaa
     3661 tggtgaagcg ccatggctga tttctcctgg caagccgaac tcgttgcccg cccggccgac
     3721 acgccgctgc tgctcgacag ccagcgcagc tggaatgccg gcgagatcgt cgccgaactg
     3781 ctcaacctgc gcgaacgcct ggccggcagc cgcgtcgtcg gcgtgcttgc cgacaacagc
     3841 ccggcctgga tcatcgccga tctcgcctgc caggacgccg gccgcgtaca cctgccgctg
     3901 cccgcctttt tccacacgga acaactgcgc aacgcgctcg aacaggccgg cgccgacacc
     3961 gtgctcaccg accagccgga acgcatcggc gccctcgacc tcggcttcgc gatcaccggt
     4021 cgctggcagg gcctgacctg gatgcgccgt gtggtcgaac cggtcgcgct gccgccgggc
     4081 accgccaaga tctccttcac ctcgggcagc accggcgccc ccaagggcgt ctgcctgagc
     4141 cgcgacggcc tgctcgacac ggcccgcgca gtccacgagc gcctcgccga cctgccgctc
     4201 gaacgccatc tggcggtatt gccgctggcc ctgctgctgg aaaacgtcgc tggcgtctat
     4261 gcgccgctgc tgcgcggcat gccggtatac ctgccgccgc tggccgaact cggctgggcc
     4321 ggcatgcagg gtttcgaccc ggcagcgctc gaccgcaagg tcaaggaaac cggcgccggc
     4381 agcgtgatcc tggtgcccga attgctcaag gcctgggcaa cttaccttgg tttcacccgc
     4441 agccagccgt cggccacgct gcaattcgtt gccgtcggcg gcgctcgcgt cgccccggaa
     4501 ctgctggccg gtgcgcgcgc cctcggcatt cccgcctacc agggctacgg cctgaccgaa
     4561 ggcggctcgg tactggcgat caaccgcccc ggcgacgacg ccgacggcgt cggccgcccg
     4621 ctcggccacg cccggctggc catcgaggat ggcgaattga ccgtgcgcac ccgcgccttc
     4681 ctcggctaca ccggacaggc ggcgaccagc gacgccgcct tcgccaccgg cgacctcgcc
     4741 agcatcgacg gcaacggtca cctgcac
//
LOCUS       DAOSDX010000088         4765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6330_length_4765_cov_4.281316, whole genome shotgun sequence.
ACCESSION   DAOSDX010000088 DAOSDX010000000
VERSION     DAOSDX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_4765_cov_4.281316"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLN02_02900"
     CDS             <1..613
                     /locus_tag="PLN02_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOI51297.1"
                     /translation="IHGGEADILVGTQMLAKGHDFPKLTLVGVLGADSALYAADWRAP
                     ERLFAQLMQVAGRAGRAELEGEVLIQTQYPEHPLFAALVAQDYPGFAAGQLKEREQAG
                     FPPYAFQAVLRAEAPAMADAMAFLAQAVVLPPVAEHEQVMLYDPVPMRMARLANLERA
                     QLLVEAGSRPALQAFLPRWRAAIEQLKQPARLRWHLEVDPLEL"
     gene            complement(621..2927)
                     /locus_tag="PLN02_02905"
     CDS             complement(621..2927)
                     /locus_tag="PLN02_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI51298.1"
                     /translation="MIGRLSLRWKLLLPLLLAALLVALLMDRFWLQRSLANIEQAQLQ
                     SAQRHLDSVGESLLPLIMGQQLDIINENLDRLLEKNPEWVAITLIDADGRQLYPLLIE
                     SEETRLGNSPDLRRISLPLDFAGRNLARIEALYDLAPQMNLQRREHRSMSLILLGVLA
                     GSVLLLWILVERVVHRPLRYLSQAASELARRNYDTTLPPGGDDDLGRLIESFERMRRE
                     LQNHHAMLDQEIAERRQAEEGLRMFSLAVEQSPASILITDTNSRIEYVNAAFVRTTGF
                     RRDEVLGENPRLLHSGKTALETYASLRRALAYGETWAGEFHNRRRDGSEFIQAAIITP
                     LREPDGKVSHYVAVQEDITEKKRLAAELDKHRHHLEELVARRTRELGEAKAAAEQANQ
                     AKSAFLANMSHEIRTPLNAIGGMAHLMRRGGLPPEQLERLDKLDAASRHLLETINTVL
                     DLSKIEAGKLMLEEIAFNPVSLLENVRSMLLEKAESKNLAFTIERTPLPERLLGDPTR
                     LQQCLLNYASNAIKFTERGSIALRADVIDQTESRVLLRFAVSDTGIGIPVEAQSRLFS
                     AFEQADTSTTRRFGGTGLGLAITAKIAEAMGGAVGLDSAPARGSSFWFTARLRKAPDL
                     GAAPHEAPAEPEEDIRQHCLNCRVLLVEDEPVNREVALELLAETGLSADSAEDGEEAV
                     AMVAANEYQLVLMDMQMPRMDGLEATRRIRRLPGREKLPIVAMTANAFAEDKAHCFEA
                     GMNDFASKPIDPELLYRLLRKWLPRRCG"
     gene            complement(2924..3763)
                     /locus_tag="PLN02_02910"
     CDS             complement(2924..3763)
                     /locus_tag="PLN02_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOI51299.1"
                     /translation="MKSLSRRIVRQFFLAACVLLGTTLPASAKESYTFSVVPQFERRM
                     LFSIWQPIVDQLERRTGLHLHLVTSLSVSDYESDVQKGQYDFIYLNPYMMPVVAETPG
                     YIPLVRDARPIHGILVVRKDSPLQKVSELQGKTLAVPSMTALGASLLLRAELDRQFGV
                     RTKVIVAKTHSSVFLHVINGFADAGGSVQKTLGEQDPRIQGALRVLHRTAAVPSHPLA
                     AHRRVPVEVREKLRQAFIELASTAEGRALLEKVPMLSPVAAQIGDYDVLKSLKLENYL
                     EQP"
     gene            3898..>4765
                     /locus_tag="PLN02_02915"
     CDS             3898..>4765
                     /locus_tag="PLN02_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51300.1"
                     /translation="MKIETSAVNLTASHEASYDRRLEVASQSGFRQIFESMAGTGDES
                     AAELRERVQRMLDALVGAIIAAMDGRQCRQDDAAWEPLPAQAEENRNGREFAWQSTIS
                     ERIEESESTRVCASGKVNTCDGRELAFDLSLDMSRHYEKTSVLSESGSVRLRDPLVLS
                     FPGQACELSEKRFSFDIDCDGKVECLPELGGGSCFLVFDRNGNGRADDGSELFGASSG
                     DGFADLAAYDLDGNGWIDEGDAAFDKLALWSGERFESLATAGVGALYSGAVEAPFSLK
                     SPDNALLGQIRAA"
BASE COUNT          744 a         1483 c         1660 g          878 t
ORIGIN      
        1 tatccatggc ggcgaggccg acatcctggt cggcacgcag atgctggcca aagggcacga
       61 ttttccgaag ctgacgctgg tcggcgtgct cggcgccgac tcggcgctct acgccgccga
      121 ctggcgcgcg ccggagcgcc tgttcgcgca gttgatgcag gttgccgggc gggccggccg
      181 cgctgaactg gaaggcgagg tgctgatcca gacccagtat cccgaacatc ccttgtttgc
      241 cgcgctggtc gcccaggatt acccgggttt cgccgctggc cagctcaagg aaagggaaca
      301 ggcgggtttc ccgccctacg ccttccaggc ggtgctgcgt gccgaggcgc cggcaatggc
      361 cgacgcgatg gctttcctgg cgcaggcggt ggtgctgccg ccggtcgccg agcacgagca
      421 ggtcatgctt tacgatccgg tgccgatgcg catggcccgc ctggccaacc tggagcgcgc
      481 ccagttgctg gtcgaagccg gttcgcgtcc ggcgctgcag gccttcctgc cgcgctggcg
      541 ggcggcgatc gagcagctga aacagccggc gcgcctgcgc tggcatctcg aagtcgatcc
      601 gctggaactc tgagggtcgt tcagccgcag cgccgcggca gccacttgcg caacaggcgg
      661 tagagcagtt cggggtcgat cggcttgctc gcgaaatcgt tcatgccggc ctcgaaacaa
      721 tgtgccttgt cctcggcgaa ggcgttcgcc gtcatcgcca cgatcggcag tttttcccgg
      781 ccgggcaggc ggcgaatgcg gcgggttgcc tcgaggccgt ccatgcgcgg catctgcatg
      841 tccatcagga ctagctggta ttcgttggcg gcaaccatgg cgacggcctc ttcgccgtct
      901 tcggcggagt cggcgctgag gccggtttcg gccagcagtt cgagggcgac ctcgcggttg
      961 accggctcgt cctcaacgag caggacgcgg cagttcaggc aatgctgccg gatgtcttct
     1021 tctggctcgg caggcgcttc gtggggcgct gcgccgagat cgggcgcctt gcgcaggcgg
     1081 gcggtgaacc agaagctgct gcccctggcg ggggcgctgt ccagacccac cgcgccgccc
     1141 atcgcttcgg cgatcttggc ggtgatcgcc aggcccaggc cggtgccgcc gaagcgccgg
     1201 gtggtcgatg tgtcggcctg ctcgaaggcg gagaacagcc ggctctgggc ttcgaccgga
     1261 atgccgatgc cggtgtcgct gaccgcgaag cgcagcaaca cgcggctctc ggtctggtcg
     1321 atgacgtcgg cgcgcagagc gatgctgccc cgttcagtga atttgatcgc gttgctggcg
     1381 taattgagca ggcactgctg cagccgggtc gggtcgccaa gcaggcgttc cggcagcggg
     1441 gtgcgttcga tggtgaaggc cagatttttg ctctctgcct tttccagcag catcgagcgg
     1501 acgttttcga gcaggctgac cggattgaaa gcgatttcct cgagcatcaa cttgccggcc
     1561 tcgatcttcg acaggtcgag caccgtgttg atggtttcca gcagatgccg gctcgcggca
     1621 tcgagcttgt cgagccgttc gagctgttcc ggcggcaggc cgccacggcg catcaggtgg
     1681 gccatgccgc cgatggcgtt gagcggggta cggatttcgt ggctcatgtt ggcgaggaag
     1741 gcgcttttcg cctggttggc ctgttcggcg gcggccttgg cctcgcccag ctcgcgggtg
     1801 cgccgggcaa ccagctcttc gaggtgatga cgatgcttgt ccagttcggc ggccaggcgc
     1861 ttcttttcgg tgatgtcttc ctgcaccgct acgtaatggc tgacctttcc gtcgggttcg
     1921 cgtagcgggg tgatgatcgc cgcctggatg aattcgctgc cgtcccggcg ccggttgtgg
     1981 aactcgcccg cccaggtttc gccgtaggcc agcgcccgcc gcaggctggc gtaggtttcc
     2041 agggcggtct tgccggagtg cagcaggcgc gggttttcgc ccaagacttc gtcgcggcgg
     2101 aagccggtgg tgcggacaaa agccgcgttc acgtactcga tgcgcgagtt ggtgtcggtg
     2161 atcaggatgc tggccggact ctgctcgacc gccagcgaga acatgcgcag gccttcctcg
     2221 gcctggcggc gctcggcgat ttcctggtcg agcatggcgt ggtggttctg cagttcgcgg
     2281 cgcatccgct cgaaactttc gatcaaccgg ccgaggtcgt cgtcgccgcc cggcggcagc
     2341 gtcgtgtcgt aattgcgccg ggcaagttcc gaggcggcct gcgacaggta gcgtagcggc
     2401 cggtgcacga cccgttcgac caggatccac aggagcagca cgctgccggc caggacaccg
     2461 agcaggatca ggctcatcga ccggtgttcg cgacgctgca ggttcatctg cggcgccaga
     2521 tcgtaaaggg cctcgatgcg ggccagattg cgaccggcga agtccagggg cagcgagatg
     2581 cggcgcaggt cggggctgtt gccgagccgg gtttcctcgg actcgatcag cagcgggtag
     2641 agttgtcgtc cgtcggcatc gatcagggtg atcgccaccc attccgggtt tttctcgagc
     2701 aagcggtcga gattctcgtt gatgatgtcg agctgctgtc ccatgatcag cggcaacagg
     2761 ctttcgccga cgctgtcgag atggcgctgc gccgattgca gttgcgcctg ctcgatgttg
     2821 gccaggctgc gctgcagcca gaagcggtcc atcagcaggg caaccagcag cgccgccagc
     2881 agcagcggca ggagcagctt ccagcgcagc gagagcctgc cgatcacggt tgttccaggt
     2941 agttttccag cttcagcgac ttcagtacgt cgtagtcgcc gatctgtgcc gccaccggcg
     3001 acagcatcgg taccttttca agcagggcac gcccttccgc ggtgctggcc agttcgatga
     3061 aggcctggcg cagcttttcc ctgacctcga ccggcacccg ccggtgcgcg gcaagcggat
     3121 gcgaggggac ggccgcggtc cggtgcagca cgcgcagggc gccctgaatc cgcgggtcct
     3181 gttcgcccag cgtcttctgc accgagccgc cggcgtcggc gaagccgttg atcacatgca
     3241 ggaagaccga actgtgcgtc ttggcgacga tgaccttggt cctgacgccg aattgccggt
     3301 cgagttcggc gcgcagcagg aggctggcgc caagcgcggt catcgacggc acggccagcg
     3361 tcttgccctg caattcgctg accttctgca gcgggctgtc cttgcgtacg accaggatgc
     3421 cgtggatcgg cctggcatcg cgcaccagcg gaatgtagcc gggcgtctcg gcgaccaccg
     3481 gcatcatgta ggggttgagg tagatgaagt cgtactgacc tttctgcacg tcgctttcgt
     3541 agtcgctgac cgacagcgag gtgaccagat gcaggtgcag gccggtgcgt ctttccagtt
     3601 ggtcgacgat gggttgccag atgctgaaca acatgcgccg ctcgaactgc ggcacgaccg
     3661 agaaggtata gctttccttg gcggaggcgg gaagggtggt gccgagcagc acgcaggctg
     3721 ccaggaaaaa ttgccggacg atccggcggc tgagggactt catggctgct gccttgtggt
     3781 gttaccggtt tactgccgat tctccgtctc tcggcgaggg ctgtccatcg gactttgggc
     3841 gctgcccggc acgtcccttg cgggtgtggt gacccgaaca cagcccggag ggtgatcatg
     3901 aaaatcgaaa catccgccgt caacctgacg gcaagccatg aagccagtta cgatcgtcgg
     3961 ctcgaagtcg cctcccagag cggcttccgg cagatattcg aaagcatggc ggggactggc
     4021 gacgaaagcg ccgcggaact cagggagcgg gtccagcgca tgctcgacgc gctggtcggg
     4081 gcgatcattg cggcaatgga tggccggcaa tgccggcagg acgatgccgc ctgggagccc
     4141 ttgccggccc aggcggaaga aaaccggaac ggccgcgaat tcgcctggca gagcacgatc
     4201 agcgagcgca tcgaggaaag cgagagcacc cgcgtctgcg ccagcggcaa ggtcaatacc
     4261 tgcgacggcc gcgagctggc cttcgacctc tcgctcgaca tgtcgcgtca ttacgagaaa
     4321 accagcgtgc tctcggaaag cggcagcgtc cgcctgcgcg acccgctggt gttgagcttt
     4381 cccggccagg cctgcgagtt gtcggaaaaa cgtttcagtt tcgacatcga ctgcgacggc
     4441 aaggtcgaat gcctgcccga actgggtggc ggtagctgct tcctggtctt cgaccgcaac
     4501 ggcaacggcc gagccgacga cggcagcgaa ctgttcggtg ccagcagcgg cgacggcttc
     4561 gccgacctgg cggcctacga cctcgacggc aacggctgga tcgacgaggg cgatgccgcg
     4621 ttcgacaagc tcgccctgtg gtcgggtgag cgtttcgaat cgctcgccac ggctggtgtc
     4681 ggcgccttgt atagcggggc ggtagaggcg ccgttctcgc tcaagtcgcc ggacaacgcc
     4741 ctgctcgggc agatccgcgc ggccg
//
LOCUS       DAOSDX010000089         4698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6431_length_4698_cov_3.406634, whole genome shotgun sequence.
ACCESSION   DAOSDX010000089 DAOSDX010000000
VERSION     DAOSDX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6431_length_4698_cov_3.406634"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLN02_02920"
     CDS             complement(<1..397)
                     /locus_tag="PLN02_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI51301.1"
                     /translation="MKGRLTLSVLVALLGLILLGTFQVLHLRAQLLEDRKATLNAAID
                     IATSTIKAFQEQEAKGELSREQAQKHATDALRGMRYLGPEYFYIYDSKGMNVMHPVRP
                     EYVGKSHWDRQDKQGNYTVRDLVKTALDKT"
     gene            596..4540
                     /gene="hrpA"
                     /locus_tag="PLN02_02925"
     CDS             596..4540
                     /gene="hrpA"
                     /locus_tag="PLN02_02925"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOI51302.1"
                     /translation="MSANFRKPASRDDVSLPTLTADARRLRALQRDLPRSTGEKRQKL
                     QADLERLLAASQAAVGERRGQLPKPEFQPDLPVNERRDEIAELIARHQVVIVCGETGS
                     GKTTQLPKICLDLGIGARGLIGHTQPRRLAARSVATRLAQELKTQVGAGVGVKIRFHD
                     KSTPESWVKLMTDGILLAESQSDPYLNAYEAIIIDEAHERSLNIDFLLGYLKQLLPRR
                     PDLKLIITSATIDAERFSQHFSGAPVIEVSGRLYPVEVRHRPVEDIDKKDDERDLYDA
                     IVDAADELARLGSGDILVFLPGEREIREAAEALRKHALARPGLSTAHAPEILPLFSRL
                     SASEQDRIFKPSGGQRRIVLATNVAETSLTVPGIRYVIDTGLARVKRYSYRNKVEQLQ
                     VEKISQAAARQRAGRCGRVAAGVCIRLYDEADFNARPPFTDPEILRSSLAGVILRMKS
                     LRLTDVESFPFIEPPPAKAISDGYALLQELGALDDENRLTKVGEALAKLPLDPRIGRM
                     LVAARDLGCLSEVLVIAAGLSTQDPRERPQERQQAADEKHKLFADEKSEFLSWVKLWA
                     WFKQAVEHKKSNKSLVDTCHAHFLSYLRLREWREVHGQLHAMVAELGWKENEIPGSYD
                     AIHMALLSGLLGNIGCKSDESGYYLGARGIRFLIHPSSPLQKKAGKWIMAAEITETTR
                     LFGRCVARIEDAWIEKVGAHLIKRQYFDPHWEKKAMQVAGWERTTLYGIVINPKRRIH
                     YGPLAPAEAREIFIRQGLVGEEIEESFAKRWPFFQHNQKQIREIEKIEHKQRRQDVLV
                     DDELIFAFYDHLIPEDIHNGATFDHWRKTAEQDNPKLLFLAKDDLMRHSAAGVTTEAF
                     PHKLKAGGVEYALTYHFEPGSPRDGVTLTLPLAQLNQIPAARMEWLVPGLIKEKLVQL
                     IKTLPQKIRAKLVPVPEFVEEFLAAVEGNDRKLNQGIINPLIEFILEARGLNARGWAV
                     TPDAFRPDALPPHFSMNYKLIDEHGRQLDMNRSLVALRGEWGKEAKEEFAELHETPSE
                     YTQLTDWTFGELPELMEVPVGKQTVIGYPALIDDGETVSLKVFDSAEEAADAHRKGLS
                     RLFMLQFKEQVKYFDKNVPGLTQMAMQFMALGSSDDLKRQLVELTFERACLSEPLPTT
                     PEAFKARCAEAKARLGLIMQEVCRLVGTVLSEWQAAVKKLPAFKAHAAAVADIEAQLK
                     RLIGKNFVVDTPFERLQHYPRYLKAVGVRLDKLKANPARDAQLMAEYAPLWTNYERRA
                     IQLAKMGSVDPQIEQFRWLLEELRVGLYAQELRTPVPVSVKRLQKQWEGMAR"
BASE COUNT          874 a         1538 c         1501 g          785 t
ORIGIN      
        1 cggtcttgtc gagcgcggtc ttgaccagat cgcggacggt gtagttgcct tgcttgtcct
       61 ggcggtccca gtgactcttg ccgacgtatt ctggacggac cggatgcatg acgttcatgc
      121 ccttgctgtc gtagatgtaa aagtactccg gtccaaggta gcgcatgccg cgcagcgcgt
      181 cggtcgcgtg cttctgcgcc tgctcgcggc tgagttcgcc tttcgcttcc tgttcctgga
      241 aagccttgat cgtgctcgtg gcgatgtcga tggcggcgtt cagcgtcgcc ttacggtcct
      301 cgagcagttg tgcgcgcagg tgcagaacct ggaaggtgcc gagcaggatc aggccaagca
      361 gggcgaccag gacggacagg gtcaggcgcc ccttcatgct ggcaaaactg aataaattca
      421 cgattttgtc tccaagcgtt tgctgatttt ttcagctttg cattttctgg cgacaagagt
      481 atgacatcct ggctgacctg tcgatacgtg atcttgctta gatgaccatc tttccggcgg
      541 aggagtggga ttgcgccagt ctgtccgcct cgtcgggcga atataattcg cccccatgtc
      601 cgccaacttc cgcaagcccg cgtcgcgcga cgacgtttcc ctgcccaccc tgactgccga
      661 tgcccgccgc ctgcgcgcgt tgcagcgcga cctgccacgg tcgaccggcg aaaaacggca
      721 aaaactgcag gccgatctgg aaaggctgct cgccgcctcg caggccgccg tcggcgaacg
      781 gcgcggccag ctgccgaagc cggaatttca gcccgacctg ccggtcaatg agcggcgcga
      841 cgaaatcgcc gaactgatcg ccaggcatca ggtcgtcatc gtctgcggcg agaccggctc
      901 gggcaagacc acccagttgc cgaaaatctg cctcgatctc ggcatcggcg cccgcggcct
      961 gatcggccat acgcagccgc gccggctggc cgcgcggtcg gtggcgacgc gcctggcgca
     1021 ggaactgaag acccaggtcg gtgccggcgt cggcgtgaag atccgcttcc acgacaaatc
     1081 gacgccggag agctgggtca agctgatgac cgacggcatc ctgctggccg agtcgcaaag
     1141 cgacccctac ctgaatgcct acgaggcgat catcatcgac gaggcgcacg agcgcagcct
     1201 gaacatcgac ttcctgctcg gctacctgaa gcagttgctg ccgcgccggc cggacctcaa
     1261 gctcatcatc acctcggcga ccatcgatgc cgagcgtttc tcgcagcatt tcagcggcgc
     1321 gccggtgatc gaggtgtccg gccgactcta cccggtcgaa gtgcgccatc gcccggtcga
     1381 ggacatcgac aagaaggacg acgagcgcga cctctacgat gccattgtcg acgccgctga
     1441 cgaactggcc cggctgggtt ccggcgacat cctcgtcttc ctgcccggcg agcgggaaat
     1501 ccgcgaggcg gcggaggcac tgcgcaagca tgccttggct agaccgggct tgagcaccgc
     1561 gcacgcgccg gaaatcctgc cgctgttctc gcgcctgtcg gcttccgagc aggaccgcat
     1621 cttcaagccc tcgggcggcc agcggcgcat cgtgctggcg accaacgtcg ccgaaacctc
     1681 gctcaccgtg ccgggcatcc gctacgtcat cgacacgggc ctggcgcggg tcaagcgcta
     1741 ctcctaccgc aacaaggtcg agcagttgca ggtcgagaag atttcgcagg cggcagcgcg
     1801 gcagcgcgcc ggccgctgcg gccgcgtcgc cgccggcgtg tgcatccgct tgtacgacga
     1861 ggccgatttc aacgcgcggc cgccgttcac cgacccggaa atcctgcgtt cctcgctggc
     1921 cggcgtcatc ctgcgcatga agtcgctgcg cctgaccgat gtcgagagct tccccttcat
     1981 cgagccgccg ccggccaagg cgatcagcga cggctacgcg ctgttgcagg aactcggcgc
     2041 gctcgacgac gaaaaccgcc tgaccaaggt cggcgaggcg ctggccaagc tgccgctcga
     2101 cccgcgcatc ggccgcatgc tggtcgccgc gcgcgatctc ggttgtctga gcgaagtgct
     2161 ggtcatcgcc gccgggctgt ccacgcagga cccgcgcgag cggccgcagg aacggcagca
     2221 ggcggcggat gagaagcaca agctgttcgc cgacgagaag tccgaattcc tcagctgggt
     2281 caaattgtgg gcctggttca agcaggcggt cgagcacaag aaatcgaata aatcgctggt
     2341 cgatacctgc cacgcgcatt tcctctccta cctgcgcctg cgcgaatggc gcgaggtgca
     2401 tgggcagttg cacgcgatgg ttgcggaact cggctggaag gaaaacgaga ttcccggcag
     2461 ctacgacgcc atccacatgg cgctgctgtc cgggctgctc ggcaacatcg gctgcaagtc
     2521 ggacgagtcc ggctactacc tcggcgcgcg tggcatccgc ttcctcatcc acccgtcgtc
     2581 gccgctgcag aagaaggccg gcaagtggat catggccgcc gagatcaccg agacgacgcg
     2641 cctcttcggg cgctgcgtcg cccgcatcga ggatgcctgg atcgagaaag tgggcgctca
     2701 cctgatcaag cgccagtact tcgacccgca ctgggaaaag aaggcgatgc aggtcgccgg
     2761 ctgggaacgc acgaccctct acgggatcgt catcaacccc aagcggcgta tccattacgg
     2821 cccgctggcg ccggccgagg cgcgcgagat cttcatccgt caggggttgg tcggcgagga
     2881 gatcgaggag agttttgcca agcgctggcc cttcttccag cacaaccaga agcagatccg
     2941 cgagatcgag aagatcgagc acaagcagcg ccgccaggac gtgctggtcg atgacgaact
     3001 gatcttcgcc ttctacgatc acctgatccc ggaagacatc cacaacggcg ccaccttcga
     3061 ccactggcgc aagaccgccg agcaggacaa cccgaagctg ctcttcctgg ccaaggacga
     3121 cctgatgcgc cattcggcgg ccggcgtcac caccgaggcc ttcccgcaca aactcaaggc
     3181 cggcggtgtg gagtacgcgc tcacttatca cttcgagcct ggctctccgc gtgacggcgt
     3241 caccctgacg ctgccgctgg cgcaactcaa ccagattcca gcggcgcgca tggaatggct
     3301 ggtgccgggg ctgatcaagg aaaagctggt gcagttgatc aagacgttgc cgcagaaaat
     3361 ccgcgccaag ctggtgccgg tgccggaatt cgtcgaggag ttcctcgccg ctgtcgaagg
     3421 caacgaccgc aagctcaacc agggcatcat caatccgctg atcgaattca tcctcgaagc
     3481 gcgcgggctg aacgcgcgcg gctgggcggt gacgcccgac gccttccgcc ccgacgcgct
     3541 gccgccgcac ttctcgatga actacaagtt gatcgacgaa cacggccggc aactcgacat
     3601 gaaccgcagc ctggtcgcgc tgcgcggtga atggggcaag gaagccaagg aggaattcgc
     3661 cgaactgcac gaaacgccgt ccgaatacac ccaactgacc gactggactt tcggcgaact
     3721 gccggagctg atggaagtgc cggtcggcaa gcagacggtg atcggctatc cggcgctgat
     3781 cgacgacggc gaaacggtca gcctgaaagt cttcgattcc gccgaggaag ccgccgacgc
     3841 gcaccgcaaa ggcttgtcgc gcctgttcat gctgcagttc aaggagcagg tgaagtactt
     3901 cgacaagaac gtccccggcc tgacccagat ggcgatgcag ttcatggcgc tgggcagcag
     3961 cgacgacctg aagcggcagc tcgtcgaact gaccttcgag cgtgcctgcc tgagcgaacc
     4021 gctgccgacc acgccggaag ccttcaaggc gcgttgtgcc gaggccaagg cgcggctcgg
     4081 gctgatcatg caggaagttt gccgcctggt cggcaccgtg ctcagcgaat ggcaggcggc
     4141 ggtcaagaag ctgccggcgt tcaaggcgca cgcggcggcc gtcgccgaca tcgaggcgca
     4201 gttgaagcgg ctgatcggca agaacttcgt cgtcgacacg cccttcgagc gtctgcagca
     4261 ctacccgcgc tacctgaagg cggtcggcgt gcggctcgac aagctcaagg ccaaccccgc
     4321 ccgcgacgcg caattgatgg ccgagtacgc gccgctatgg accaactacg agcggcgggc
     4381 gatccagttg gcgaagatgg gttcggtcga cccgcaaatc gagcagttcc gctggctgct
     4441 ggaagaacta cgcgtcggcc tctacgcgca ggagttgcgc acgccggtgc cggtgtcggt
     4501 gaagcggctg cagaagcaat gggaagggat ggctcgctga gtcaggccat gacgctgatg
     4561 ctgccggcga tgctgctcag cgtcagcgtc agcgtcagcg tcggcgccat cccggcggcg
     4621 gcaccgagtc cgccatagag gtcgccgccg cccttgagcg cggtatcgac ctggtgcagc
     4681 gcattttccg ccgctctg
//
LOCUS       DAOSDX010000090         4680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6463_length_4680_cov_4.050595, whole genome shotgun sequence.
ACCESSION   DAOSDX010000090 DAOSDX010000000
VERSION     DAOSDX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6463_length_4680_cov_4.050595"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..649)
                     /locus_tag="PLN02_02930"
     CDS             complement(203..649)
                     /locus_tag="PLN02_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011254973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51303.1"
                     /translation="MTARKSEFEKLLGRVVSQGVARADSGLLREVAEKLWYGRSVPNL
                     SKFPEPERREAGYLLDRLARVNVMAVARKQKVLAALTPYKPSTPDLQVGHRDVLAVTW
                     GAREELTPRMAELLPYQTRTYEAERKAHAKRLRAQAAKEVLPGKKP"
     gene            complement(646..1230)
                     /locus_tag="PLN02_02935"
     CDS             complement(646..1230)
                     /locus_tag="PLN02_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011254972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HOI51304.1"
                     /translation="MTPMYRAILQLFANLAADLAARNLPEHSVHAYVFGGCAVHMHTR
                     SRASSDVDVEFDYDAMVKVSEVRLVLEQLPPVDYDDPVTGPSQLIWDANFNTTFGPLH
                     EDYQARAQEIERAPGSPVVVWLPSAEDLALSKLGRFADVDQNDILELLSLPESSWELF
                     ESLARDVSGYYVGPPLDSKIAYIRKRLEKHRKKP"
     gene            1287..1682
                     /locus_tag="PLN02_02940"
     CDS             1287..1682
                     /locus_tag="PLN02_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51305.1"
                     /translation="MWQQNLALQGEPQTPPELMQHLALQLIGGNGSAQPWRLTRGSEH
                     WEGLPNGPLAANSPGLQREMAAHGLGIIGLDERMARPWVERGLLQRILPDWSLPSVTL
                     WCVPPGRRLIPSRTTAFIELLRAALVGER"
     gene            complement(1750..1926)
                     /locus_tag="PLN02_02945"
     CDS             complement(1750..1926)
                     /locus_tag="PLN02_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51306.1"
                     /translation="MPGGGFKKLTRLPTKGKHCAGNTNPANPVTAMNLTMEFGGDFGE
                     KSSLAASGCGLPLG"
     gene            complement(1999..3399)
                     /locus_tag="PLN02_02950"
     CDS             complement(1999..3399)
                     /locus_tag="PLN02_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI51307.1"
                     /translation="MKRILAVDGLSVRAGDNALLRDVGFALAPGEALTLVGQSGAGKS
                     LLAQAIMGNLPDALQAAGTVEVAGVASAAADHHTRRPLWGRQLGLLPQEPWLALDPTM
                     RILAQVEETYRHVRRDGNAANARQRAREALAGLGLQDKAGHYPHQISGGMAQRVAFAA
                     VYAGGAPILIADEPTKGLDRRMRDEVVTLLREALLEGCALLTVTHDLEVAEALGGRLA
                     VIEQGRIVESDATTTVMQAPQHPYTQALLAAQPRNWPAEPAPASGQPLLTAQGLAKAF
                     GRQRLFAGIDLCVHAGERLALTGPSGCGKTTLGNILLGVLKPDAGRVVSHAARPFAFQ
                     KLYQDPVAAFPARQTLRQTFVDLCRLHRLDTGAAAPLLAELGLDTALLDRLPGEVSGG
                     ELQRIAIARILLLQPQLVFADEPTSRLDAITQKQTMAVLLRALERSGAALVLVTHDDA
                     LAERVAGRRLAIDTAY"
     gene            complement(3396..4184)
                     /locus_tag="PLN02_02955"
     CDS             complement(3396..4184)
                     /locus_tag="PLN02_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI51308.1"
                     /translation="MKLTTLHRIGWLLLAAVAVFAAFGPALTGTDPFAQDLRAFLQAP
                     GLAHPLGTDHLGRSMLARLADAAQLSLGLAALSVLSAAIPGTVLGILAAWYGGALEKV
                     LLFISDSVLALPGLLLVLLLATLAPGEFWPLYLGLSLAFWVEYFRVVRASARTLFASP
                     GVEASLLLGFGLGYIVRRHVWPELAPVVRTLASFGLAAAVLAMAALGFVGVGVKPPRP
                     ELGVMMTELLPYYQEAPWLIAAPVGLLATTLLALILIQHEEKNA"
     gene            complement(4196..>4680)
                     /locus_tag="PLN02_02960"
     CDS             complement(4196..>4680)
                     /locus_tag="PLN02_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI51309.1"
                     /translation="PPFLLGLLMVIVFAGADLPAAGHGEAANLFLPALTLALGLAAGS
                     SRIVREAIRNVLASEHYAFAQTKGLPLSLAVWRHALRNMALPVVAYLGVQLVFVIEGV
                     VILETLFAWPGIGHALVHAVFWRDVPMIQGTCLMLALCFVLLNALVDLLCLALDPRHR
                     "
BASE COUNT          847 a         1558 c         1553 g          722 t
ORIGIN      
        1 tggcccgccc ctgggtcgaa cgcggcctgc tccagcgcat cctcccggac tggtcactac
       61 cctcggtcac cctctggtgc gtcacccccg gccgccgcct gatcccgagc cggacgacgg
      121 cgttcattga attgctaagg gcagcgctga ttggggagcg gtgagtgcga cagtgggttt
      181 gctttgccgg ccagcgcgaa cgctacggct ttttacccgg caagacctct ttggccgcct
      241 gcgcccgcag cctctttgcg tgcgcctttc tctcggcttc ataggttcgc gtctggtacg
      301 gcaaaagttc ggccatacgt ggcgtcagct cttcgcgagc cccccaggtg acggccaata
      361 cgtctctgtg cccgacttgc aaatcgggag tggatggctt atagggagtc aaagccgcca
      421 acactttctg cttgcgcgcc accgccatca cattcacgcg cgccaggcga tcaagcagat
      481 agcccgcttc gcggcgctcg ggctcgggaa atttcgaaag attgggcacg ctgcgaccat
      541 accagagctt ttcggcgacc tcgcgtagca acccggaatc agcacgagcg acaccttggc
      601 tcaccactcg gccgaggagc ttttcgaatt cggatttacg agcggtcacg gtttcttcct
      661 gtgtttttct aggcgttttc gaatgtaagc gattttagaa tcaagcggcg ggccgacata
      721 gtagccgctg acatcccgtg ccagcgactc gaacagctcc caacttgatt cgggcagcga
      781 cagcagttcg agaatgtcat tctggtcgac atccgcaaag cgaccgagtt tcgacagggc
      841 aaggtcttcg gcactcggca gccacacgac gaccggagaa ccgggcgccc gctcgatctc
      901 ttgggcacgc gcctggtaat cctcgtgcag cggaccaaag gtggtgttga agttggcatc
      961 ccagatcagt tgagatggac cagtgactgg gtcgtcataa tccaccggcg gcaactgctc
     1021 gagtaccaga cgaacctccg acaccttgac catggcatcg tagtcaaact ccacatcgac
     1081 atcggaactc gccctggacc gtgtgtgcat atgcacggca caaccgccga aaacatacgc
     1141 atgcactgaa tgctcaggga gattgcgtgc ggcaagatcc gctgcaagat tcgcaaatag
     1201 ctgcaggatt gcgcggtaca tcggggtcat attgatctgc ggcgcgatgt ggaatggctg
     1261 attttagcgc ttagccatca gacaacgtgt ggcaacagaa tctcgcgctc caaggcgagc
     1321 cgcaaacgcc gcccgagctg atgcaacatc tcgccctgca actgatcggc ggcaacggca
     1381 gcgcccagcc ctggcgcctg acgcgcggca gcgaacactg ggaaggcctg cccaacggcc
     1441 cgctcgccgc caactcaccc ggcctgcaac gcgaaatggc cgcgcacggc ctcggcatca
     1501 tcggcctcga cgagcgcatg gcccgcccct gggtcgaacg cggcctgctc cagcgcatcc
     1561 tcccggactg gtccctgccc tcggtgacgc tctggtgcgt cccccccggc cgcaggctga
     1621 taccaagccg gacgacggcg ttcattgaat tgctgagggc ggcgctggtt ggggagcggt
     1681 gaatgatctg cctgaggagt ttccttgccg acctccttgc cagatattcc gctttggcga
     1741 aatccggtct catcccaatg gcagcccgca gccagaagcg gccaatgacg atttctcccc
     1801 aaaatcgcca ccaaattcca ttgtcaaatt cattgcagtt acagggtttg ccggatttgt
     1861 attgccggcg cagtgtttcc ctttcgtcgg caggcgcgtc agtttcttaa agccgccacc
     1921 cggcattttc aggccgacga ttcatcttcc tcgcccaatc cggcaaacag gctccgctca
     1981 cacagccaca taacgcgttc agtacgccgt gtcgatagcc aggcgccgtc cggcaacccg
     2041 ttcggccaaa gcatcgtcgt gggtgaccag gaccagcgcc gcgccgctgc gctcaagggc
     2101 gcgcagaagc acggccatgg tctgcttctg ggtgatcgcg tcgaggcgcg aggtcggctc
     2161 gtcggcaaag accagttgcg gctgcagcag caggatgcgg gcgatcgcga tgcgctgcag
     2221 ttcaccgccc gaaacttcgc ccggcaggcg gtcgagcaag gcggtgtcga ggccgagctc
     2281 ggcgagcagc ggcgccgccg caccggtatc gaggcgatgc aggcggcaca ggtcgacgaa
     2341 ggtttgccgc agcgtctgcc gcgccgggaa ggcggcgacc gggtcctggt acaacttctg
     2401 gaaggcgaac gggcgcgctg catggctgac gacacgaccg gcgtcgggct tgagcacgcc
     2461 gagcaggatg ttgcccagcg tcgtcttgcc gcaaccgctc gggccggtca gcgccagccg
     2521 ctcgccggca tggacacaga gatcgatacc ggcgaacagc cgctgccggc cgaaagcctt
     2581 ggccaggccc tgcgccgtca gcagcggctg gccgctggcc ggcgccggct ctgccggcca
     2641 gttgcgcggc tgggcagcga gcagcgcctg ggtgtaggga tgctgcggcg cctgcatcac
     2701 cgtcgtcgtg gcatcggatt cgacgatccg cccctgttcg atcaccgcca ggcggccgcc
     2761 gagggcctcg gcgacttcca ggtcgtgggt caccgtgagc agcgcacagc cttcgagcag
     2821 ggcctcgcgc agcaaggtga cgacctcgtc gcgcatgcgc cggtcgaggc ccttggtcgg
     2881 ttcgtcggcg atcaggatcg gcgcgccgcc ggcatagacc gcggcgaagg cgacgcgctg
     2941 tgccatgcca ccggaaatct ggtgcggata atgcccggcc ttgtcctgca ggccgaggcc
     3001 ggccagcgcc tcgcgcgccc gctggcgggc gttggcggcg ttgccgtcgc ggcggacatg
     3061 gcgataggtt tcctcgacct gggccaggat gcgcatggtc gggtcgagcg ccagccaggg
     3121 ctcctgcggc agcaggccga gctggcgacc ccacagcggc cggcgcgtgt ggtggtcggc
     3181 ggcggccgag gcgacgccgg cgacctcgac cgtgccggcc gcctgcaggg catccggcag
     3241 attgcccatg atcgcctgcg ccagcagcga tttgccggcg ccgctctggc cgaccagggt
     3301 cagcgcttcg cccggagcca gcgcaaaacc cacgtcgcgc agcagcgcgt tgtcgccggc
     3361 gcgaacgctc agcccgtcaa ccgccaggat gcgcttcatg cgttcttctc ctcgtgctgg
     3421 atcaggatca gcgccagcaa ggtcgtcgcc agcaggccga ccggcgcggc gatcagccag
     3481 ggggcttcct ggtagtacgg cagcagctcg gtcatcatca cgccgagctc cgggcgcggc
     3541 ggcttgacgc cgacgccgac gaagccgagc gcggccatcg ccagcaccgc ggcggccagg
     3601 ccgaagctgg ccagcgtccg caccaccggc gccagttccg gccagacgtg acggcggacg
     3661 atgtagccga gaccgaaacc gagcagcaac gaggcctcga cgccgggcga ggcgaacagc
     3721 gtccgggcac tggcgcggac gacgcggaaa tactcgaccc agaaggccag cgacaggccc
     3781 aggtaaagcg gccagaactc gcccggcgcc agtgtcgcca gcagcagcac cagcagcagg
     3841 ccgggcagcg ccagcaccga atcggagatg aacagcagca ccttttccag cgcgccaccg
     3901 taccaggccg cgaggatgcc gagcacggtg ccgggaatcg ccgccgacag cacgctcagc
     3961 gcggccagcc cgagcgagag ctgggcggca tcggcgagcc gggcgagcat gctgcggccg
     4021 aggtggtcgg tgccgagcgg atgcgccagg ccgggcgcct gcaggaaggc gcgcaggtcc
     4081 tgggcgaagg gatcggtacc ggtgagcgcc ggcccgaagg cagcgaagac tgcgacggcg
     4141 gcgagcagca gccagccgat gcgatgcagg gtagtcagct tcatgggcgg ttcattcagc
     4201 gatggcgcgg atcgagcgcc aggcaaagca ggtcgaccag cgcgttcaac aggacgaagc
     4261 acagcgccag catcaggcag gtgccctgga tcatcggcac gtcgcgccag aacaccgcgt
     4321 gcactagtgc atgaccgatg ccgggccagg cgaacagggt ttccaggatg accacgcctt
     4381 cgatgacgaa aaccagctgg acgccaagat aggcgaccac cggcagcgcc atgttgcgca
     4441 gtgcgtggcg ccacaccgcc agcgacagcg gcaggccctt ggtctgcgcg aaggcgtaat
     4501 gctcgctggc gagcacgttg cggatcgcct cgcggacgat gcgcgacgag ccggcggcga
     4561 ggccgagcgc cagtgtcagc gccggcagga acaggttggc agcttcgccg tgtccggccg
     4621 ccggcaggtc ggcgccggcg aagacgatga ccatcagcag cccgagcagg aaaggcggaa
//
LOCUS       DAOSDX010000091         4675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6473_length_4675_cov_3.063636, whole genome shotgun sequence.
ACCESSION   DAOSDX010000091 DAOSDX010000000
VERSION     DAOSDX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_4675_cov_3.063636"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLN02_02965"
     CDS             complement(<1..598)
                     /locus_tag="PLN02_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOI51310.1"
                     /translation="MRFDKFTTMFQQALADAQSLANGNDQQFIEPQHLLLALLVQDDG
                     GTASLLSRAGVNVAGLKRDLEQALTRLPKVEGHGGDVQVGRDLGNLLNLTDKEAQKRG
                     DQFIASEMFLLALCDDKGETGRIAKQHGLQKKALEAAIMAVRGGQNVDSQEAEGQRES
                     LKKFCIDLTERAAQGKLDPVIGRDDEIRRAIQILQRRTK"
     gene            755..2380
                     /locus_tag="PLN02_02970"
     CDS             755..2380
                     /locus_tag="PLN02_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI51311.1"
                     /translation="MAKSMTLRGKLTAMTLATIGALIVLFAVLLVSGKQRMLEDREAK
                     VRNLVEATHALVAHFHAESVAGRLTDAEARKAAADAVRAIRYDQVEYFWINDLDDLMV
                     MHPIKPELDGKKLDQLKDKNGKLLFLEFNTVVKNQGAGYVEYLWPKPGHEEGVPKISY
                     VMGFKPWGWVIGSGIYIDDVDARFREDAAILLLWGLAIGGFIAVSLMLVSRSIIKTLG
                     GDPSAASAVTRRIAAGDLSSPVDCPPGKNLLSDIRAMQDTLRAMISTIVSNAEHLASA
                     ADQLLSSSDEVADRARQQSDAASSMAASVEQMAVSIDQVKENAAEAHGISREAGNISE
                     EGAAVIHNAASEMRKISEAVQSSSQIVEDLGHRSDQITSIVNTIREIADQTNLLALNA
                     AIEAARAGEQGRGFAVVADEVRKLAERTSLSTTEIAEMVTKIQNGTRSAVSSMQEGVV
                     KVGNGVQLANQAGDSIERIRDGAQRVTFVVNGISDSISEQSQASNDIAQKLETIAQMS
                     EESAIAVRQTADAARRLHKLSGELHQAVAQFKT"
     gene            complement(2387..3340)
                     /locus_tag="PLN02_02975"
     CDS             complement(2387..3340)
                     /locus_tag="PLN02_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI51312.1"
                     /translation="MDHIDYPAFRRLDLNLLVAFDALLREGGVSRAAARLCIGQPAMS
                     HALARLRELFQDDILYREGAGMQPTAHALALAPRIRRLLAEAQAVTRSAAEFDPAQVT
                     GDFRLALTDPLEALLLPGLVARIRARAPGLSLSVRPIPASRQLEHLDAGNVNLAVGHF
                     PDAREVHEVQLLFRAGFCCVFNPALLDMPSPIGLADLVRHPHIHTSYTGDAPGLTDRL
                     LADAGLRRHVVAQAASPLAIPFVVKQSPLLAVVPDLVARLFAAHADLAIEPLAVDRLV
                     LPISRVSHRRDRGDPLTGFVADQVAQAAAALFGETAAGNSR"
     gene            3425..>4675
                     /locus_tag="PLN02_02980"
     CDS             3425..>4675
                     /locus_tag="PLN02_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI51313.1"
                     /translation="MSATPTPQVSGLLRCIAAITCIEFFETGLVMFGATHIMAGLGLS
                     PEQFALAYTLYGVSSIYMLYKHQWMVERLGYRRFVLASLGVFALGGILAATAGGFGQF
                     ACGRLLQGASGATFFTAGRLAINRLPAEARFPGLLAFIGSLLGASAVAPVAAAVLLAA
                     FDWRALFWAALPLCLLIARIAGPHLDQSTVPPEQRSEEHWGWLLWLVIGIFGLQYAIQ
                     EIPAAGAAAHRLVFVVGLASIAALTVFAWRQWRKERPLIDYRGLLQWRYLLGIALYFS
                     GYFMLGAFGFLLPIFFHNGLGLSLASTAVILGLSAAGSVVAAFVHAWAARRVASQRHF
                     MLGGLGLYALACLAFSQAGRLADWPLLLLPALVCGVAVPFYIGPVAFGTFSELPARVF
                     SHAYQVKNIVRQLGISSSIALSTAG"
BASE COUNT          798 a         1600 c         1459 g          818 t
ORIGIN      
        1 tcttggtccg gcgctgcagg atctggatgg cgcggcggat ttcgtcgtcg cggccgatca
       61 ctgggtcgag cttgccctgg gcggcgcgtt cggtgaggtc gatgcagaac ttcttcagcg
      121 attcgcgctg gccctcggct tcctgcgaat cgacattctg gccgccgcgg acggccatga
      181 tcgccgcttc cagcgccttc ttctgcaggc cgtgctgctt ggcgatgcgg ccggtttcac
      241 ccttgtcgtc gcacagggcg agcaggaaca tctcgctggc gatgaactgg tcgccacgct
      301 tctgcgcttc cttgtcggtg agattgagca ggttgcccag gtcgcggccg acctggacat
      361 cgccgccgtg cccctcgacc ttgggcagcc gggtcagcgc ctgctccagg tcgcgcttga
      421 gacccgccac attgacgcca gcccgggaca acaaggatgc ggtaccgccg tcgtcctgga
      481 caagcagtgc cagcagcagg tgctgcggtt caatgaactg ttggtcgttg ccattggcca
      541 ggctctgtgc gtcggccagt gcctgctgga acatggtggt gaatttgtca aatcgcatgc
      601 tgtgctcccg cggtaatttc tcgatagccc acagatgcgt ccgtcccgcc agttttcaag
      661 taccgttcgg cactttacgc aataacccct attgcctcat ggactagcga gtgttagctt
      721 cccctttgca gagcaattcc cacaagagga aaaaatggca aaatcaatga cactgcgcgg
      781 caagctgacc gcgatgacac tggccaccat cggcgcactg atcgtcctgt tcgccgtcct
      841 gctggtcagc ggcaagcagc gcatgcttga ggacagggag gccaaggtgc gcaatctggt
      901 cgaggcaacc cacgcactgg tcgcccactt ccacgccgag tcggtggccg gccgcctgac
      961 cgatgccgag gccagaaaag ccgccgcaga cgccgtgcgg gcaatccgct acgaccaggt
     1021 cgagtacttc tggatcaacg atctcgacga cctgatggta atgcacccga tcaagcccga
     1081 actcgacggc aagaagctcg accagctcaa ggacaagaac ggcaagttgc tgttcctcga
     1141 attcaacacc gtggtgaaga accagggtgc cggctacgtc gagtacctgt ggcccaagcc
     1201 cggccatgag gaaggcgtac cgaaaatatc ctacgtcatg ggcttcaagc cctggggctg
     1261 ggtgatcggt tccggcatct atatcgacga cgtcgatgcc aggttccgcg aagacgccgc
     1321 catcctgttg ttgtggggcc tagccatcgg cggcttcatc gccgtctcgc tgatgctggt
     1381 ctcgcgcagc atcatcaaga ccctcggcgg cgatccctcg gccgcctccg ccgtcacccg
     1441 gcgcatcgcc gccggcgacc tctcgtcgcc ggtcgattgc ccgcccggca aaaacctgct
     1501 ctccgacatc cgcgccatgc aggacacctt gcgagcgatg atctcgacca tcgtcagcaa
     1561 cgccgagcac ttggccagcg ccgccgacca gttgctcagt tcgtccgacg aggtcgccga
     1621 ccgcgctcgc caacaaagcg atgctgcgtc gtcgatggcc gcctcggtcg agcagatggc
     1681 agtgagcatc gaccaggtca aggaaaacgc tgccgaagcc cacggcatct cgcgcgaggc
     1741 cggcaacatc tcggaagaag gcgccgcggt gatccacaac gcggccagcg agatgcgcaa
     1801 gatttccgag gcggtgcaat cctcgtcgca aatcgtcgaa gacctcggcc atcgctcgga
     1861 ccagatcacc tccatcgtca acaccatccg cgagatcgcc gaccagacca atctgctggc
     1921 gctcaacgcc gccatcgagg cggcccgcgc tggcgaacag ggacgcggtt tcgccgtggt
     1981 cgccgacgaa gtgcgcaagc tggccgaacg gaccagtctt tcgacgaccg agatcgccga
     2041 aatggtgacc aagatccaga acggcacgcg cagtgcggtg agcagcatgc aggaaggcgt
     2101 cgtcaaggtc ggcaacggcg tccagttggc caatcaggcc ggcgattcga tcgagcgcat
     2161 ccgcgacggc gcgcagcgcg tcaccttcgt ggtgaacggc atttccgact cgatttcgga
     2221 gcagagccag gccagcaacg acatcgcgca gaagctcgaa accatcgccc agatgtccga
     2281 ggaaagcgcc atcgccgtcc gccagacggc cgacgccgcg cgccgcctgc acaagctgtc
     2341 gggcgaactg caccaggccg tcgcccagtt caagacctga ggcgatttac cgcgaattgc
     2401 cagccgccgt ttcgccaaac agggcggcgg ctgcctgcgc cacctgatcg gcgacgaagc
     2461 cagtcagtgg atcgccccgg tcgcgccgat ggctgacgcg cgaaatcggc aagaccagtc
     2521 tatccaccgc cagcggctcg atcgccagat cggcatgggc cgcgaacagg cgcgccacca
     2581 ggtcgggaac caccgccagc agcggactct gcttgaccac gaaggggatc gccagcggcg
     2641 acgccgcctg cgccacgacg tgacgccgca ggccggcatc ggccagcagg cggtcggtca
     2701 gccccggcgc atccccggta tagctggtat ggatgtgcgg atggcggacc aggtcggcga
     2761 ggccgatcgg cgacggcatg tcgagcaggg ccggattgaa gacgcagcag aagccggcgc
     2821 gaaacagcaa ctgcacctca tgcacctcgc gcgcgtcggg aaaatggcca accgcgagat
     2881 tgacgttgcc ggcatcgaga tgttcgagct ggcgtgaggc gggaatcggc cgtaccgaca
     2941 ggctcaggcc cggcgcccgg gcccggatgc gggcgaccag gccgggcagc agcaaggctt
     3001 caagcggatc ggtgagggcc aggcggaagt cgccggtgac ctgcgccgga tcgaattccg
     3061 ccgcgctgcg ggtcaccgcc tgcgcttccg cgagcagacg gcggatgcgc ggcgccaggg
     3121 ccagggcgtg ggcggtcggc tgcatgccgg cgccttcgcg gtagaggatg tcgtcctgga
     3181 acagttcgcg cagacgggcc agggcatgac tcatcgccgg ctggccgatg cacaggcggg
     3241 cagcggcccg gctgacaccg ccctcgcgca gcagggcgtc gaaggcaacc agcaggttga
     3301 gatcgagacg gcgaaaggcc gggtaatcaa tatgatccat atcgatagtc tagatcatat
     3361 caatcgattt cgtgcattca agcagcgctt ttaaactgtc aggcttttcc tccgcccgat
     3421 accgatgtcc gccaccccta cgccgcaggt cagcggcctg ctgcgctgca ttgccgcgat
     3481 cacctgcatc gagttcttcg aaaccggcct ggtcatgttc ggcgccaccc acatcatggc
     3541 cggccttggc ctcagccccg aacaattcgc gctggcctac acgctgtacg gcgtcagctc
     3601 gatctacatg ctctacaagc accaatggat ggtcgaacgc ctcggctatc gccgtttcgt
     3661 tctcgcttcg ctgggcgtct tcgcgcttgg cggcatcctc gccgcgaccg ccggtggctt
     3721 cggccagttt gcctgcggcc gcttgctgca gggcgccagc ggcgccacct tcttcaccgc
     3781 cgggcggctg gcgatcaatc gcctgcccgc agaggcgcgc ttccccggcc tgctcgcctt
     3841 catcggcagc ctgctcggcg cttctgccgt ggcgccagtg gccgctgccg tgttgcttgc
     3901 cgccttcgac tggcgggcgc tgttctgggc ggcgctgccg ctctgtctgc tgatcgcccg
     3961 gatcgccggc ccccacctcg accaaagtac cgttcccccc gaacagcgca gcgaggaaca
     4021 ctggggctgg ctgctctggc tggtgatcgg cattttcggc ctgcagtatg cgatccagga
     4081 aatcccggca gccggcgccg cagcgcatcg cctggtcttc gtcgtcggcc tcgcctcgat
     4141 cgccgcgctg accgtcttcg cctggcggca gtggcgcaag gaaaggccgc tgatcgatta
     4201 ccgcggtctg ctgcaatggc gctacctgct ggggattgcc ctgtatttct cgggctattt
     4261 catgctcggc gccttcggct tcctgctgcc gatcttcttt cacaacggcc tcggcctgtc
     4321 gctcgccagc accgccgtga tcctcggcct gagcgccgcc ggcagcgtcg tcgccgcctt
     4381 cgtccatgcc tgggcggcgc gccgggtagc cagccagcgc cacttcatgc tcggcggcct
     4441 cggtctttac gccctcgcct gtctcgcctt cagccaggcc ggacgcctcg ccgactggcc
     4501 gctcctgctg ctgcctgcac ttgtctgcgg cgtcgctgtg cccttctaca tcggcccggt
     4561 cgctttcggc accttcagcg aactgccggc gcgcgtcttt tcgcacgcct accaggtcaa
     4621 gaacatcgtc cgtcagctcg gcatttccag cagcatcgcg ctatccaccg ccggc
//
LOCUS       DAOSDX010000092         4663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6486_length_4663_cov_3.941406, whole genome shotgun sequence.
ACCESSION   DAOSDX010000092 DAOSDX010000000
VERSION     DAOSDX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6486_length_4663_cov_3.941406"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..733)
                     /gene="fliJ"
                     /locus_tag="PLN02_02985"
     CDS             complement(275..733)
                     /gene="fliJ"
                     /locus_tag="PLN02_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOI51314.1"
                     /translation="MSSTFSLQPLLELMQEKTDEATRRLGQLIAAEQNARSRLQMLEN
                     YRAEYAERLRQAISQGITRQVLANYQDFLARIDEAILQQRKIVSQSENNTARGQQDWQ
                     EKNTRLKAIGTLSDRHDERERRKEDKKEQKLLDEFTTRKFATRSKDGTPE"
     gene            complement(737..2155)
                     /gene="fliI"
                     /locus_tag="PLN02_02990"
     CDS             complement(737..2155)
                     /gene="fliI"
                     /locus_tag="PLN02_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOI51315.1"
                     /translation="MAAALNESPIDHGGRWQKFLDGCRLAAGNAQPLWPSGRLTRING
                     LVMEASGLKLPLGSTCRIYPTIGAPVEAEVVGFAGEKLYLMPSDEVFGLASGARVVPF
                     DAPSPAPQIDQPGHLKRRAIDRAKQVPVGDELLGRVVDGVGRPLDGQGPLHAAHARPL
                     QSRPINPMSRAAIEHPLDVGIRAINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTE
                     AEVIVVGLIGERGREVKEFIEQILGEEGRRRSVVVAAPADTGPLMRLQGAAYATTIAE
                     YFRDQGRQVLLIMDSLTRYAMAQREIALAIGEPPATRGYPPSVFARLPQLVERAGNGP
                     EGGGSITAFYTVLAEGDDQQDPIADSARAILDGHIVLTRSLADAGHYPAIDIEQSISR
                     AMVNLIDNHQLDNIRRFKVLFSRYQRSRDLIAVGAYVPGSDPVLDQAVANYPNMEAFL
                     QQRIDEKSDFPTGVAHLAQLFM"
     gene            complement(2140..2853)
                     /locus_tag="PLN02_02995"
     CDS             complement(2140..2853)
                     /locus_tag="PLN02_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOI51316.1"
                     /translation="MAGVIPKEQLAAYQRWQANAFDAPAAPPAAPAREAEAAPGAALP
                     EAEVESVSAAAFALPTADEIEQMHEQAHDEGYRAGYEEGLQAAQAATLAAREQEVARL
                     AAMTGALREALNRLDQEVAEQLLDLALEVAAQVVGSSLKTQRELLLPVIREALQALPL
                     HHGSIALHANPDDLENLRDALDELVAQSNLKLVPDSAIAPGGCLLKAGHSEVDASLAT
                     RWKRVLEAIGVDPDKWLLR"
     gene            complement(2857..3855)
                     /gene="fliG"
                     /locus_tag="PLN02_03000"
     CDS             complement(2857..3855)
                     /gene="fliG"
                     /locus_tag="PLN02_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOI51317.1"
                     /translation="MPADDGLEKSATLLIALGEDHAAEVLKHLGPREVQKLGHAMAAL
                     KSVPRTTVEAVLDNFHKDIEEHSAVHVDTDNYIRSVLTKALGDDKASNLISRILQGGE
                     TSGIEGLKWMDAQTVADLIKNEHPQIIATILVHLEHDHASEILNHFTERLRNDVVLRI
                     ATLEGIQPSALRELNEAMLRILSGSASIKRTAMGGVRTVAEILNFIGTANETSVVDAI
                     REYDPDLAQKILDEMFVFENLMDIDDRSIQMILREVQSDSLVLALKGASPELREKIFK
                     NMSQRAAEMLREDLESKGPVRLSEVEAEQKEILKVVRRLADEGQIVLGGAGGEQMI"
     gene            complement(3845..>4663)
                     /locus_tag="PLN02_03005"
     CDS             complement(3845..>4663)
                     /locus_tag="PLN02_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HOI51318.1"
                     /translation="NETASVNEATGGVPGALTNQPPVPATAPLTAPATGNTAAAGQRP
                     GNIPGNIDAAGITAPINAAGQPISTAKNSTVNFELDRTIRHTKQGMGAVRRLTAAVVV
                     NHRKDVDAKTGKDITKPLADNEMKQINELVREAMGFSQERGDSISVANAPFTAVEKEE
                     ISLPVWKDPEMLGVGKEVLQYLLIVGIIAFLYFKIIQPALQTMFPPPPKAAETAATAG
                     AAGHLGAAGETGEEEENVEIDHYAIKVQKARDLAQNDAKAVANIIKEWMGANAS"
BASE COUNT          622 a         1420 c         1668 g          953 t
ORIGIN      
        1 aggacttgcc gtccgtggct tcgcttgggc ccagggggct gctcaggtgg ccttgcaaca
       61 gcccggcaaa gccgccggcc agcgattccc caccggcgtt ctgggaattg ccggtgtttt
      121 tgccgtggcg gccgtggatg gtcgagtgca gtaaggcttc gatgcccatg atgattcctg
      181 gtttatgctt gcactagggg ttcagcaatt aacgtgccgg gtcgcgggct tgttccgctc
      241 aaccgcgttc tccgccgccg attgcgcacg cccgctattc cggcgtgccg tctttcgacc
      301 gggtggcgaa tttccgggtg gtgaattcgt ccagcagctt ttgttccttc ttgtcttcct
      361 tgcgccgctc gcgttcgtcg tggcggtcgg agagtgtacc gatcgccttc agacgggtgt
      421 tcttttcctg ccagtcctgc tggccgcggg ccgtgttatt ttccgactgg ctgacgattt
      481 ttcgctgttg caggatcgcc tcgtcgatgc gggccaggaa gtcctggtag ttggccagca
      541 cttgtcgggt gatcccttgc gagatggcct ggcgcagccg ctccgcgtat tcggcacggt
      601 agttctcgag catctgcagg cgactgcggg cgttctgctc ggcggcgatg agctggccaa
      661 ggcggcgggt ggcttcgtca gtcttttcct gcatcagttc gagcaggggc tgcagggaga
      721 aggtggaact catggcttac atgaaaagct gggcgaggtg ggcgaccccg gtcgggaaat
      781 cggatttctc gtcgatccgc tgttgcagga aggcttccat gttcggatag ttggccacgg
      841 cctggtcgag caccgggtcg gagccgggaa cgtaggcgcc gaccgcgatc aggtcgcgcg
      901 agcgctggta gcgcgagaac aggaccttga agcggcggat gttgtcgagc tggtggttgt
      961 cgatcaggtt gaccatggcg cggctgatcg actgttcgat gtcgatcgcc gggtagtggc
     1021 cggcatcggc caggctgcgg gtgagcacga tgtggccgtc gaggatggcg cgcgccgagt
     1081 cggcgatcgg gtcctgctgg tcgtcgcctt cggcaagtac ggtgtagaag gcggtgatcg
     1141 aaccgccgcc ttccggcccg ttgccggcgc gctcgaccag ttgcggcagg cgggcgaaaa
     1201 ccgacggcgg atagccgcgg gtcgccggcg gttcgccgat ggccagggcg atttcccgtt
     1261 gcgccatggc gtagcgggtg agcgagtcca tgatcagcag gacctggcgg ccctgatcgc
     1321 ggaagtattc ggcgatcgtc gttgcgtagg cggcgccctg caggcgcatc agcggtccgg
     1381 tatcggccgg ggcggcaacg acgacggaac ggcgccggcc ttcctcgccg agaatctgct
     1441 cgatgaactc cttgacttcg cgaccgcgct cgccgatcag gccgacgacg atcacttcgg
     1501 cttcggtgta acgcgccatc atgccgagca gcaccgactt gccgacgccg gaaccggcga
     1561 ataggcccat gcgctggccg cggccgacgg tgagcagagc gttgatggcg cggatgccga
     1621 cgtctagcgg gtgttcgatc gccgcccggc tcatcgggtt gatcggccgg ctctgcagcg
     1681 gccgggcgtg cgcggcgtgc agcggtccct ggccgtcgag cgggcggccg acgccgtcga
     1741 cgacgcgacc gagcagttcg tcgccgaccg gcacctgctt ggcgcggtcg atggcgcgcc
     1801 gcttgaggtg gccgggttgg tcgatctgcg gggccgggct gggggcgtcg aagggaacga
     1861 cgcgggcgcc ggaggcgagg ccgaagactt cgtcggacgg catcaggtag agcttttcgc
     1921 cggcaaagcc gacgacttcg gcttcaacgg gggcgccgat ggtcggatag atgcggcagg
     1981 tgctgccgag cggcagtttg agcccggaag cctccatgac caggccgttg attcgggtca
     2041 gacggccgct cggccagagc ggctgcgcat tgccggcagc gaggcggcag ccgtcgagga
     2101 atttctgcca gcgtccgccg tggtcgatcg ggctttcgtt caacgcagca gccatttgtc
     2161 gggatcgacg ccgatcgctt cgagcacccg cttccagcgc gtcgccaggc tggcatcgac
     2221 ttcgctgtgg ccggccttga gcaggcagcc gccgggggcg atggcgctgt ccggcaccag
     2281 cttgaggttg ctctgggcga ccagctcgtc gagcgcgtcg cgcaggtttt ccaggtcgtc
     2341 cgggttggcg tgcagggcaa tgctgccgtg atgcagcggc aacgcctgca gcgcttcgcg
     2401 gatcaccggc agcagcagct cgcgctgggt tttcaggctg ctgccgacga cctgcgcggc
     2461 tacttccagc gccaggtcga gcaattgctc ggcgacttcc tggtcgaggc ggttcagcgc
     2521 ctcgcgcagg gcgccggtca tcgccgccag gcgggcgacc tcctgctcgc gcgcggcgag
     2581 cgttgccgcc tgggcggctt gcaggccttc ttcgtaaccg gcgcggtagc cctcgtcatg
     2641 ggcctgttcg tgcatctgct cgatttcgtc ggcggtcggc agggcgaagg cggcagcgct
     2701 gacgctttcg acttccgctt ccggcagcgc tgcgcccggc gccgcttccg cttcgcgcgc
     2761 gggcgccgcc ggcggcgcgg cgggagcgtc gaaggcgttg gcctgccagc gctggtacgc
     2821 cgccaactgt tccttgggaa tgacgccggc catgctttag atcatctgct cgccgccagc
     2881 gccgccgagg acgatctgtc cttcgtcggc cagtcggcgg accaccttga ggatttcctt
     2941 ctgttccgcc tcgacttcgg agaggcggac cggacccttc gattcgagat cctcgcgcag
     3001 catttcggcg gcgcgctgcg acatgttctt gaagatcttt tcgcgcagtt ccggcgaggc
     3061 gcccttcagc gccagcacca gcgagtcgga ctggacttcg cgcaggatca tctggatcga
     3121 gcggtcgtcg atgtccatca ggttttcgaa gacgaacatc tcgtcgagga tcttctgcgc
     3181 caggtcgggg tcgtactcgc ggatcgcgtc gaccaccgag gtttcgttgg cggtgccgat
     3241 gaagttgagg atttcggcga cggtacgcac gccgcccatc gccgtccgct tgatgctggc
     3301 cgagccggag aggatgcgca gcatcgcctc gttcaactcg cgcagcgccg aaggctggat
     3361 accttccaga gtggcgatgc gcagcacgac gtcgttgcgc aggcgctcgg tgaagtggtt
     3421 gaggatttcg ctggcgtggt cgtgttcgag gtgcacgagg atggtggcga tgatctgcgg
     3481 gtgctcgttc ttgatcaggt cggcgacggt ctgtgcgtcc atccacttca ggccttcgat
     3541 gcccgaggtt tcgccgccct gcaggatgcg cgagatcagg ttggaggcct tgtcgtcgcc
     3601 gagcgccttg gtcagcaccg agcggatgta gttatcggtg tctacatgga cggccgaatg
     3661 ttcctcgatg tccttgtgaa agttgtcgag cacggcctcg accgtggtcc gcggcacaga
     3721 cttgagggcg gccatcgcat ggccgagctt ttgcacctcg cgcgggccga ggtgcttgag
     3781 cacttcggcg gcgtggtcct cgcccagcgc gatgagcagg gtcgcgctct tttccagtcc
     3841 gtcgtcagct ggcattggcg cccatccatt ccttgatgat gttggctacc gccttggcgt
     3901 cgttctgcgc caggtcgcgg gccttctgta ccttgatcgc gtagtggtcg atttccacgt
     3961 tttcctcttc ctcgcctgtc tcgccagcgg cgcccaggtg gccggccgcg ccagcggtgg
     4021 ctgcggtttc ggcagccttc ggcggaggcg ggaacatggt ctggagcgcc ggctggatga
     4081 tcttgaagta gaggaaggcg atgatgccga cgatcagcag gtactgcagc acttccttgc
     4141 cgacgcccag catttccgga tccttccaca ctggcaggct gatttcttcc ttctcgacgg
     4201 cggtgaacgg cgcgttggcg accgagatcg aatcgccgcg ttcctggctg aagcccatcg
     4261 cttcccggac cagttcgttg atctgcttca tttcgttgtc ggcgagcggc ttggtgatgt
     4321 ccttgccggt cttggcgtcg acatccttgc gatggttgac gacgacggcc gcggtcaggc
     4381 ggcgcaccgc gcccatgccc tgcttggtgt gacggatggt gcggtcgagt tcgaaattga
     4441 cggtcgagtt cttggctgtg ctgatcggct ggccggcggc gttgatcggc gccgtgatgc
     4501 cggcggcgtc gatgttgccc gggatattgc cgggacgctg gccggcggcg gcagtgttgc
     4561 cagtggccgg ggcggtcagc ggcgcggtgg cggggaccgg cggctggttg gtcagcgcgc
     4621 cgggcacgcc gccggtggct tcgttgacgc tggcggtctc gtt
//
LOCUS       DAOSDX010000093         4643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6513_length_4643_cov_3.346992, whole genome shotgun sequence.
ACCESSION   DAOSDX010000093 DAOSDX010000000
VERSION     DAOSDX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6513_length_4643_cov_3.346992"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PLN02_03010"
     CDS             complement(<1..419)
                     /locus_tag="PLN02_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOI51319.1"
                     /translation="MQKKIIALAVAGLASTAAFAQSNVQIYGVVDVGYNRSSSSDTGA
                     LKSRNALDSGLQSGSRIGFRGTEDLGNGLKASFVLEYGITADTGAGLSGPARQSFLAL
                     SNSYGTVAAGRQYTPQYELVSKVDPFGAGTVGDVTFGR"
     gene            complement(594..1382)
                     /gene="trpC"
                     /locus_tag="PLN02_03015"
     CDS             complement(594..1382)
                     /gene="trpC"
                     /locus_tag="PLN02_03015"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOI51320.1"
                     /translation="MSDILNKIIATKREEIAAGLALKPLAALEAEAAQQAAPRDFVGA
                     IRAKLACGQAAVIAEIKKASPSKGVIRADFRPADIARSYAEHGAACLSVLTDRQYFQG
                     APEYLQAARAACSLPVLRKDFIVDAYQVAEARAMGADAILLIAAALSLPEMQELERQA
                     MDYGMAVLVEVHNGEELDAALQLQTPLLGINNRNLRTFEVSLDTTLGLLGRIPQERIV
                     VTESGILKPEDVRLMRQNAVHAFLVGEAFMRADEPGEELGRLFS"
     gene            complement(1385..1639)
                     /locus_tag="PLN02_03020"
     CDS             complement(1385..1639)
                     /locus_tag="PLN02_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOI51321.1"
                     /translation="MLIKFSDRAWDDYLYWQQSDKITLRRINSLIKDIQRSPFEGIGK
                     PEPLRHNLAGFWSRRIDEQHRLVYAIEADCILLAQCRYHY"
     gene            complement(1629..1889)
                     /locus_tag="PLN02_03025"
     CDS             complement(1629..1889)
                     /locus_tag="PLN02_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOI51322.1"
                     /translation="MEAMTYSYTRQHFADVMDRVNEDRAPLLITRQQGKPVVMMSLDD
                     FNALEETAYLLRSPANAARLIEAVGQLRSGGGEVRELRPDAD"
     gene            complement(1934..2524)
                     /locus_tag="PLN02_03030"
     CDS             complement(1934..2524)
                     /locus_tag="PLN02_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOI51323.1"
                     /translation="MALVRENAKFDAAGYLAWEAEQPNRNEYIAGEVFAMVGVRLSHN
                     VATGNLYNALRNALRGSPCRVYIESVKTRVEAADCFFYPDVVVTCDARDCATPDYISH
                     PLLVVEVLSETTAAFDRGDKFAAYRKLESLQEYVLVDLPAKRLEIFRRNAEQHWVLYE
                     YFDGDEIGFASLSLKLPVATVLEDTEEIADLPAQQA"
     gene            complement(2555..3580)
                     /gene="trpD"
                     /locus_tag="PLN02_03035"
     CDS             complement(2555..3580)
                     /gene="trpD"
                     /locus_tag="PLN02_03035"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOI51324.1"
                     /translation="MTPQAALQRVIEHREIFHDEMVSLMRQIMSGEVSPVMIAAIVTG
                     LRVKKETIGEIAAAASVMRELATPVKVPYDKHFVDIVGTGGDAAHSFNISTTSIFVAA
                     AAGAKVAKHGGRSVSSSSGSADVLEALGANINLSPEKVAECIEQCGIGFMFAPNHHSA
                     MKHVAPVRREMGVRTIFNILGPLTNPAAAPNTLMGVFHPDLVGIQVRVMQRLGAERVL
                     VVHGKDNLDEISLGATTLIGELKDGEVREYEVHPEDFGLQMMSLRNLRVGSADESKAM
                     MLAALDNQPGPAREIVCLNAGAALYVANAADSIADGIAKAREAIASGAARAKLAQFVE
                     CTQALAA"
     gene            complement(3577..4149)
                     /locus_tag="PLN02_03040"
     CDS             complement(3577..4149)
                     /locus_tag="PLN02_03040"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOI51325.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGQEVKVFRNDAITLAEIARLKP
                     DYLVISPGPCAPAQAGVSLAAIKEFAGKIPLLGVCLGHQSIGEAFGGRIVHARTLMHG
                     KVSPVHHKNEGVFRNLPNPLTCTRYHSLAIERASLPDCLDVTAWTEDGEIMGVRHKTL
                     AVEGVQFHPESILTERGHDLLNNFLEEHKQ"
     gene            complement(4160..>4643)
                     /locus_tag="PLN02_03045"
     CDS             complement(4160..>4643)
                     /locus_tag="PLN02_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOI51326.1"
                     /translation="TQLLDLGRNDCGRVAKIGSVKLTERMVIERYSHVMHIVSNVEGR
                     LEKGLDAVDVLKATFPAGTVSGAPKVRAMEIIDELEPVKRGIYAGSVGYLGFNGDMDL
                     AIAIRTAVVKDGKLHVQAGAGIVADSDPHSEWEETRNKASAVLRAAELAEQGLNTRMD
                     "
BASE COUNT          843 a         1378 c         1515 g          907 t
ORIGIN      
        1 cgaccgaagg tcacatcacc aacggtaccg gcgccgaacg gatcaacctt ggaaaccagt
       61 tcgtattgcg gggtgtattg acgaccagcg gcaacggtac cgtagctgtt ggacagagcc
      121 aggaaggact ggcgagccgg gccagacagg ccagcgccgg tgtcggcggt gatgccgtat
      181 tcgagaacga aggaagcctt caggccgttg cccaggtctt ccgtaccacg gaagccgatg
      241 cgcgagccgg attgcagacc gctgtccaga gcgttacggc tcttcagagc gcccgtgtcg
      301 gagctggaag aacggttgta accgacgtca acaacaccgt agatctgaac gttcgactgc
      361 gcgaaagcag cggtggaagc caggccagcg acggccagag cgataatctt cttttgcatc
      421 agaaaatctc cttagtggtt agtttttggc cgcccccttg cggaaccggc caagctttac
      481 ctctcggatc gagaagttga agggcattgt gcaaggggga cttgcaggcg gcaaggctta
      541 atccggagag tcactacaca ttgtgaaact ctgttgcatc gatgcaacat gactcaggaa
      601 aaaagccggc ccagttcttc gcccggctcg tcggcacgca tgaaggcctc gccgacgagg
      661 aaggcgtgaa ccgcgttttg gcgcatcaga cggacatctt cgggcttcag gatgccgctt
      721 tcggtgacca cgatgcgctc ctgcgggatg cgcccgagca ggccgagggt ggtgtcgaga
      781 ctgacctcga aggtgcgcag gttgcggttg ttgatgccga gcaagggtgt ctgcaactgc
      841 agcgcggcgt cgagttcctc gccgttgtgc acttcgacca gcacggccat gccgtagtcc
      901 atggcctggc gctccaattc ctgcatttcc ggcaggctca gcgcggcggc gatgagcagg
      961 atggcgtcgg cgcccatggc gcgggcttcg gcgacctggt aggcgtcgac gatgaagtcc
     1021 ttgcgcagca ccggcaggct gcaggcggcg cgggcggcct gcaggtattc gggcgcccct
     1081 tggaaatact ggcggtcggt cagtaccgac aggcaggcgg cgccgtgttc ggcgtagctg
     1141 cgggcgatat cggccggccg gaaatcagcg cggatgacgc ccttcgacgg gctggccttc
     1201 ttgatttcgg caatcacggc agcttggccg caggcgagct tggcgcggat ggcgccgacg
     1261 aaatcgcgcg gtgcggcctg ctgcgcggct tcggcctcaa gcgcagcaag cggcttgagc
     1321 gccagaccgg cggcgatttc ctcacgcttg gtggcgatga tcttgttcag gatgtcggac
     1381 atgttcaata gtggtaacgg cattgggcaa gcaggatgca atccgcttcg atggcataga
     1441 ccagacggtg ctgttcgtcg atgcgacgcg accagaagcc ggcaaggttg tggcgcaagg
     1501 gttcgggctt gccgatccct tcaaacgggc tgcgttgtat gtctttgatc agactgttga
     1561 tgcgccgcag ggtgattttg tcggactgtt gccagtaaag gtaatcatcc caggcgcgat
     1621 cggaaaactt aatcagcatc gggccgcaat tcccggacct caccaccacc actgcgtaac
     1681 tgccccacag cttcgatcaa gcgcgcggca ttggcgggac tgcgcaacaa ataggctgtt
     1741 tcctccagcg cattgaaatc gtcgagcgac atcatgacca ccggcttgcc ctgctgacgg
     1801 gtaatcagca agggcgcacg atcttcattg acgcgatcca tcacatcggc gaaatgctgg
     1861 cgggtgtagc tgtaggtcat ggcttccatg gcaaactcct ttgtacaaat aatcgtacaa
     1921 caactcgctt gcgtcaagcc tgctgcgccg gcagatcggc gatttcctcg gtatcctcca
     1981 gcactgtggc aacgggaagc ttcagcgata gcgaggcgaa gccgatttcg tcaccgtcga
     2041 aatattcgta cagtacccag tgctgctcgg cgttgcgacg gaagatttca aggcgtttag
     2101 ccggcaggtc aacgaggacg tactcctgca ggctttccag cttgcggtag gcggcgaact
     2161 tgtcgccgcg atcgaaggcg gcggttgttt cggaaagcac ttcgacgacc agtagcgggt
     2221 ggctgatgta gtccggcgtg gcgcagtcac gggcatcgca agtaacgacg acgtcggggt
     2281 agaagaagca gtctgctgct tcgacgcggg ttttgaccga ttcgatgtac acccggcagg
     2341 ggctgcccct gagagcattg cgcaaggcgt tgtacaaatt tccggtagca acgttatggg
     2401 acaagcgcac gccaaccatc gcaaagactt cgccagcgat gtactcgtta cggtttggct
     2461 gttccgcctc ccatgccaga tagccagcag cgtcgaattt cgcattttcc cgcaccaggg
     2521 ccatgacgaa ctcctgtggt ctgcgcttcc gtgattatgc cgccaaagcc tgcgtgcatt
     2581 cgacgaactg cgccagcttt gcccgagcgg cgccgctggc gatggcttcg cgtgctttcg
     2641 caatgccgtc ggcgatgctg tcagcagcgt tggcgacata gagcgcggcg cccgcattca
     2701 ggcaaacgat ctcgcgggcg gggcccggct ggttgtcgag ggcggcaagc atcatcgcct
     2761 tcgactcgtc ggcgctgccg acgcgcagat tgcgcagcga catcatctgc agaccgaagt
     2821 cttccggatg cacttcgtac tcgcggactt cgccgtcctt cagttcgccg atcagcgtcg
     2881 tcgcgccgag cgaaatttcg tcgaggttgt ccttgccgtg cacgaccagc acgcgctcgg
     2941 cgccgaggcg ttgcatgacg cgcacctgga tgccgaccag gtcggggtgg aagacgccca
     3001 tcagggtgtt cggcgcggca gccgggttgg tcaacgggcc gaggatgttg aagatggtac
     3061 gcacgcccat ctcgcgtcgc accggcgcga cgtgcttcat cgcgctgtgg tggttgggcg
     3121 cgaacatgaa accgatgccg cactgctcaa tgcactcggc gaccttttcc ggcgacaggt
     3181 tgatgttggc accgagcgct tcgagcacgt cggcgctacc cgagcttgac gacacgctgc
     3241 gcccgccgtg cttggccacc ttggcgccgg ccgcggcggc gacgaagatc gaggtggtgg
     3301 aaatattgaa gctgtgggcg gcgtcgccgc cggtgccgac gatatcgacg aagtgcttgt
     3361 cgtagggcac cttgaccggc gtcgccagct cgcgcatgac gctggcggcg gcggcgatct
     3421 cgccgatggt ttccttcttg acgcgcagtc cggtgacgat ggcggcgatc atcaccggcg
     3481 agacttcgcc gctcatgatc tggcgcatca gcgagaccat ttcgtcgtgg aagatttcgc
     3541 gatgctcgat gacgcgctgg agggcggctt gcggggtcat tgcttgtgct cctcgaggaa
     3601 gttgttgagc aggtcgtgac cgcgttcggt caggatcgat tccgggtgga actggacacc
     3661 ttcgacggcc agcgtcttgt ggcgcacgcc catgatctcg ccgtcctcgg tccatgccgt
     3721 gacgtcgaga caatcgggca ggctggcgcg ctcgatggcc agcgagtggt agcgggtgca
     3781 ggtcagcgga ttgggcaggt tgcggaagac gccctcgttc ttgtgatgca ccggcgacac
     3841 cttgccgtgc atcagcgttc tggcgtgcac gatgcgcccg ccgaaggctt cgccaatcga
     3901 ttggtggccg aggcagacgc cgagcagcgg aatcttgccg gcgaactcct tgatcgccgc
     3961 cagcgacacc ccggcctgcg ccggcgcgca agggccgggc gaaatgacca ggtagtcggg
     4021 cttgagacgg gcgatctcgg ccagcgtgat ggcgtcgttg cggaagactt tcacctcctg
     4081 gccaagttcg ccgaaatact ggaccaggtt gtaggtgaag ctgtcgtagt tgtcgatcat
     4141 caataacatg gcgcgcccct caatccatcc gcgtgttcag gccctgctcg gccagttcgg
     4201 cggcgcgcag cacggcgctc gccttgttcc gggtttcttc ccattcggaa tgcggatcgg
     4261 agtcggcgac gatgccggca ccggcctgga cgtgcagctt gccgtccttg accacggcag
     4321 tgcggatggc aatcgccagg tccatgtcgc cattgaagcc gagatagccg accgaaccgg
     4381 cgtagatgcc gcgcttgacc ggttccagtt cgtcgatgat ttccatcgcc cgcaccttgg
     4441 gcgctcccga cacggtaccg gccgggaagg ttgccttgag cacgtcgacc gcatcgagcc
     4501 ccttctccag ccggccctcg acgttggaga cgatgtgcat cacgtgcgag taacgctcga
     4561 tgaccatgcg ctcggtcagc ttgacgctgc cgatcttcgc cacgcggccg cagtcgttgc
     4621 gaccgaggtc gagcaactgg gtg
//
LOCUS       DAOSDX010000094         4639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6520_length_4639_cov_3.416449, whole genome shotgun sequence.
ACCESSION   DAOSDX010000094 DAOSDX010000000
VERSION     DAOSDX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6520_length_4639_cov_3.416449"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLN02_03050"
     CDS             <1..778
                     /locus_tag="PLN02_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51327.1"
                     /translation="QGQLLHAEKMVSLGRLVAGVAHELNNPISFVLGNVLSLQRYATR
                     LGRYLDAVHGSGAGEHDGLPALRDELRIDRILADMPNLIDGMIEGAERTRDIVDALKR
                     FSAVDKAAPEEVDLNEVIERSVRWVTRSGPGQFAVDLDLPPAMRCRGSSGQLQQVVMN
                     LVQNACDATADRSNARLRISGTIAEQQIVLRFADNGPGIPETNLAHIFEPFFTTKPVG
                     QGTGLGLSISYGIVERHGGRLEVHNIPDGGAEFTLTLPRH"
     gene            complement(791..2518)
                     /locus_tag="PLN02_03055"
     CDS             complement(791..2518)
                     /locus_tag="PLN02_03055"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI51328.1"
                     /translation="MALAAIKQEPAPQASAAAFLEISGVAKSYGEGKQRHEVLKDINL
                     SIREGEFVAIVGFSGSGKTTLISMIAGLIAPDTGSIQLKGKAVTEPGPDRGVVFQNYS
                     LMPWMTVRQNVALAVDQVFPEMPAAERRARVEKYIDMVSLTHAIERRPSELSGGMRQR
                     VNVARALAASPEILLLDEPLSALDALTRANLQDEILRIWEQERKTVILITNDVDEGLI
                     MADRIIPLNPGPGATFGPEFEIDLARPRDRSAMNHDPEFKRLRKEVTQYLMQMGAAQA
                     TASDNVIPLPGILPNTSADYVAGQRPAVGGAKPQSAETLPHYHRYVEFSRVSKIYSTK
                     KGPLTVVEKFDLRIRKGEFVSVIGHSGCGKSTVLSMIAGLNEISEGVALLDNREISSA
                     GPDRGIVFQAPSLIPWLTAYDNVALGVEQVYPHATAAERDAIIRHFLVRVGLADSMDK
                     LAGDLSNGMKQRVGIARAFALSPKLLLLDEPFGMLDSLTRWDLQEVLMEVWSREQLTA
                     MVITHDVDEAILLADRVVMMTNGPRAKVGKILEVDLPRPRSRKALLEHPDYYRLREEL
                     LSFLNDCNH"
     gene            complement(2533..3549)
                     /locus_tag="PLN02_03060"
     CDS             complement(2533..3549)
                     /locus_tag="PLN02_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI51329.1"
                     /translation="MLIKLANFFQQIGLTWFVPFLRMAAGDDPRGQVREILLQIGVPL
                     LALGIFLAAWNGLASGVQTSLGEIPGPAAVYEEALGLVDDHKAERAKEVAFYERQEKR
                     NAETLAADPTAEVSIRPWTGKRTYLDQIVTSLYTVFTGFAIGTLVAIPLGILCGLSRT
                     FQTALNPLIQIFRPVSPLAWLPIVTIVVSAVYVSDNPTFEKSFIVSAITVTLCSLWPT
                     LINTALGVASIDKDLINVSKVLRLSAWTKVTKIVLPSSLKLIFTGLRLSLGVGWMVLI
                     AAEMLAQNPGLGKFVWDEFQNGSSHSLGRIMVAVFTIGLVGFMLDRVMNTLQQLVSFG
                     EAGH"
     gene            complement(3649..>4639)
                     /locus_tag="PLN02_03065"
     CDS             complement(3649..>4639)
                     /locus_tag="PLN02_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOI51330.1"
                     /translation="EKLKAKLPKGADGKPEHPVKAQYLKSVVDDYRKEGKAFKMGMVF
                     PVSTHNYELRYWLASGGMNPGYYAPLKGDTAGTIGADVQLSVTPPPQMPATMEAGTID
                     GYCVGEPWNQQAVFKGIGAPVITDYEIQKNNPEKVFGVTQSWNDKYPNTHKAVVKALI
                     RAAYWLDAENNKNRQEAAKMISKSNYVGADYKVIANSMTGTFEYEKGDVRKVPDFNVF
                     FRYNATYPYYSDAIWYMTQMRRWGQIPEFKPDSWYKDMAKKVYRPDIYATAAKELIAE
                     GKMKAADFPDFAKETGFKPTQNDFIDGIPYDGSKPNAYIDSFKIGLKGKDKVK"
BASE COUNT          783 a         1443 c         1544 g          869 t
ORIGIN      
        1 gcaagggcaa ctgctgcacg ccgagaaaat ggtgtcgctc ggtcgcctgg tcgccggcgt
       61 cgcccacgaa ctgaacaacc cgatcagctt cgtcctgggc aacgtgctct cgctgcagcg
      121 ctacgcgacc cgcctcggcc gctatctcga cgccgtgcat ggcagcggtg ccggcgaaca
      181 cgacggcctg ccggcactgc gcgacgaact gcgcatcgac cgcatcctgg ccgacatgcc
      241 caacctgatc gacggcatga tcgagggcgc cgaacgcacc cgcgacatcg tcgatgcgct
      301 gaagcgtttc tcggcggtgg acaaggcagc gccggaagaa gtcgacctca acgaggtgat
      361 cgagcgctcg gtgcgctggg tgacgcgcag cggtccgggc cagttcgcgg tcgatctcga
      421 cctgccgccg gccatgcgtt gccggggttc ttcagggcag ttgcagcagg tggtgatgaa
      481 cctggtgcag aacgcctgcg acgccacggc cgaccgcagc aacgcccgcc tgcggatcag
      541 cggcacaatc gccgagcagc agatcgtcct gcgtttcgcc gacaacggcc caggaatacc
      601 ggaaacgaac ctcgcccaca tcttcgaacc cttcttcacc accaagcccg tcggccaggg
      661 caccggcctc gggttgtcga tcagctacgg catcgtcgag cggcacggcg gccgcctcga
      721 agtccacaac atcccggacg gtggggccga attcaccctc acgctgcccc gccactgacc
      781 ccgaccgcgc tcagtggttg cagtcgttga ggaagctgag cagttcctcg cgcagacgat
      841 agtagtccgg gtgctcgagc agcgccttgc gcgaacgcgg ccgcggcagg tcgacctcga
      901 ggatcttgcc gaccttggca cgcggtccgt tggtcatcat cacgacgcgg tcggcgagca
      961 ggatcgcttc gtcgacatcg tgcgtgatga ccatcgcggt cagttgctcg cggctccaga
     1021 cctccatcag gacttcctgc aggtcccagc gggtcagcga atcgagcatg ccgaacggct
     1081 cgtcgagcag cagcagcttg ggtgacaggg caaaggcgcg ggcgatgcca acccgctgct
     1141 tcatgccgtt cgacagatcg ccggccagct tgtccatgct gtcggccaga ccgacgcgaa
     1201 ccaggaaatg gcggatgatg gcgtcgcgtt cggcggccgt cgcgtgcgga tagacctgct
     1261 cgacgccgag cgccacgttg tcgtaggcgg tcagccaggg aatcaggctg ggtgcctgga
     1321 agacgatgcc gcggtccgga ccggccgaac tgatttcgcg gttgtcgagc agggcgaccc
     1381 cttcgctgat ctcgttgaga ccggcgatca tcgacaggac ggtggatttg ccgcaccccg
     1441 agtggccgat caccgagacg aactcgccct tgcggatgcg caggtcgaac ttttcgacga
     1501 cggtgagcgg tcccttcttg gtcgagtaga tcttggagac ccgcgagaac tcgacgtagc
     1561 gatggtagtg cggcagggtc tcggccgatt gcggcttggc gccgccgacc gccggccgtt
     1621 gccccgccac gtaatcggcg ctggtgttgg gcaggatgcc gggcagcgga atcacgttgt
     1681 cggacgccgt tgcctgggcc gcccccatct gcatcaggta ctgcgtcact tccttgcgca
     1741 ggcgcttgaa ttcgggatcg tggttcatcg ccgaacggtc gcgcggacgc gccaggtcga
     1801 tctcgaactc cggtccgaag gtggcaccgg gaccgggatt gagcgggatg atgcggtcgg
     1861 ccatgatgag gccttcgtcc acatcgttgg tgatcaggat gaccgtcttc cgctcctgtt
     1921 cccagatgcg caggatttcg tcctgcaggt tggcgcgggt cagcgcgtcg agcgccgaca
     1981 gcggttcgtc gaggaggagg atttccgggc tggccgccag cgcccgcgcc acattgacgc
     2041 gctggcgcat gccgccggac agctcggacg gccgccgttc gatggcgtgc gtcaggctga
     2101 ccatgtcgat gtacttctcg acccgggccc gccgttcggc cgccggcatc tcggggaaga
     2161 cctgatcgac ggcgagcgcc acgttctggc gcacggtcat ccagggcatc agcgagtagt
     2221 tctggaagac cacgccgcga tccggccccg gctcggtcac cgccttgccc ttgagctgga
     2281 tcgacccggt atcgggggcg atcaggccgg cgatcatcga aatcagcgtc gtcttgccgc
     2341 tgccggagaa accgacgatg gcgacgaatt cgccttcgcg gatgctcagg ttgatgtcct
     2401 tgagaacctc gtgccgctgc ttgccctcgc cgtaggattt ggccaccccg gaaatttcga
     2461 ggaaggctgc cgccgacgcc tgcggcgccg gttcctgctt gatcgctgcc aatgccatga
     2521 tgcgtgctcc tcttagtgac cggcttcgcc gaagctgacc agttgttgca gggtgttcat
     2581 gacgcgatcg agcatgaagc cgaccagacc gatggtgaac acggcgacca tgatgcggcc
     2641 gagggaatgc gagctgccgt tctggaactc atcccacacg aacttgccga ggcccgggtt
     2701 ctgggccagc atttcggcgg cgatcagcac catccagccc accccgaggg agaggcgcag
     2761 accggtgaag atcagcttga gcgaggacgg caggacgatc ttggtgacct tggtccaggc
     2821 cgacaggcgc agcaccttgg aaacgttgat caggtccttg tcgatcgacg ccacgccgag
     2881 cgcggtgttg atcagcgtcg gccacagcga gcacagggtg acggtaatgg cggagacgat
     2941 gaaggacttc tcgaaggtcg ggttgtcgct gacatagacc gcgctgacga cgatggtgac
     3001 gatcggcagc caggccagcg gcgacaccgg ccggaaaatc tggatcagcg ggttgagcgc
     3061 cgtctggaag gtgcgcgaca ggccgcacag gatgccgagc ggaatggcga ccagggtgcc
     3121 gatggcgaaa ccggtgaaca ccgtgtacag gctggtcacg atctggtcga gataggtccg
     3181 cttgccagtc cagggacgga tgctgacttc ggcggtcgga tcggcggcca gggtttccgc
     3241 gttgcgcttt tcctgccgct cgtagaaggc gacttccttg gcccgttcgg ccttgtggtc
     3301 gtcgaccaga ccgagcgctt cctcatagac cgcagccggt ccgggaattt cgccgaggct
     3361 ggtctgcacg ccggaagcca gcccgttcca ggcggccaga aagatgccca gggcgagcag
     3421 ggggacgccg atctggagga ggatttcccg cacctggcca cgcggatcgt cgccggccgc
     3481 catgcgcagg aaaggaacaa accaggtcag cccgatttgc tggaagaagt tggcgagttt
     3541 gatgagcatg tcgttcacct ttgttcggaa tcggcgcgcc tggcgcgcac ggttgcgggg
     3601 gcttggcggt cgccggccgg agacaggccg gcgaccggac aaagccgctt acttgacctt
     3661 gtctttaccc ttgaggccga tcttgaagct gtcgatgtag gcgttcggct tggaaccgtc
     3721 atacggaatg ccgtcgatga agtcgttctg cgtcggcttg aagcccgttt ccttggcgaa
     3781 atccgggaag tcggcggcct tcatcttgcc ttcggcgatc agctccttgg cagcggtggc
     3841 gtagatgtcc ggccggtaga cctttttcgc catgtccttg taccagctgt ccggcttgaa
     3901 ctccggaatc tggccccagc ggcgcatctg ggtcatgtac cagatggcgt cggagtagta
     3961 gggataggtc gcgttgtagc ggaagaagac gttgaaatcc ggcaccttgc gcacatcgcc
     4021 cttctcgtac tcgaaggtac cggtcatgct gttggcgatg accttgtagt cggcaccgac
     4081 gtagttggac ttggagatca tcttggccgc ttcctggcgg ttcttgttgt tttccgcatc
     4141 cagccagtaa gcggcgcgga tcagcgcctt gaccacggcc ttgtgggtgt tcggatactt
     4201 gtcgttccag gactgggtga cgccgaagac cttctccggg ttgttcttct ggatttcgta
     4261 gtcggtgatc accggcgcgc cgatgccctt gaacacggcc tgctggttcc acggttcgcc
     4321 gacgcagtaa ccgtcgatgg tgccggcttc catggtggcc ggcatctgcg gcggcggcgt
     4381 taccgagagc tggacgtcgg cgccgatggt gccggcggta tcgcccttga gcggggcgta
     4441 gtagcccgga ttcatgccgc ccgaagccag ccagtagcgc agttcgtagt tatgggtgga
     4501 caccgggaag accatgccca tcttgaaggc cttgccttcc ttgcggtaat cgtcgaccac
     4561 ggacttcagg tactgcgcct tgaccggatg ttccggcttg ccgtcggcgc ccttcggcag
     4621 cttggccttc agcttctcc
//
LOCUS       DAOSDX010000095         4635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6525_length_4635_cov_3.233624, whole genome shotgun sequence.
ACCESSION   DAOSDX010000095 DAOSDX010000000
VERSION     DAOSDX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6525_length_4635_cov_3.233624"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLN02_03070"
     CDS             <1..413
                     /locus_tag="PLN02_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOI51331.1"
                     /translation="FVAFSPLARGFLGGELSDPAAQLPPGDLRLAMPRFAPDNYARNR
                     RLLDGYRAIADEAGCSLAQLALAWVLHQQPHTLIIPGTRSPAHLADNLGAARVSLSAE
                     LIARLDALINRHTVSGPRYNAATLAEIDTERFPE"
     gene            410..1645
                     /locus_tag="PLN02_03075"
     CDS             410..1645
                     /locus_tag="PLN02_03075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI51332.1"
                     /translation="MSAAGLASRIVDRAPALGQPNFRRYFYGQICSVLGTFIQAVAMS
                     WLVYRISGSPVLLGLTAFLSQAPQLVVAPLAGVMIDRYDRRRLMIGLQCVFAAQALVL
                     AGLVFFDAVAVWHLLLLAGLLGILNSFDLPTRQSLLLYLVDDRTTLPNAVALNAAVVH
                     SGRLIGPPVAGLLLAVAPESLCFLINALSYLGPISAVLAIRAEIPRQHGLKTASALVE
                     VAAFVRSTVDARVALLAVAMVNVTASSYVVLMPVFAKEVFAGSAQSLGWLLGMGGAGA
                     LVASLYLSTRARSEIRPDFAIGSCAVAAVGLLGFALAGYVGSLALALPMVCLIGAGIG
                     ATNIAANTRLQQLAPEGLRGRVISIFGSTRFGFDALGGLLAGGLASLLGVLPVVGALG
                     LLLLPGSLALLRRLPRSPG"
     gene            complement(1617..2525)
                     /locus_tag="PLN02_03080"
     CDS             complement(1617..2525)
                     /locus_tag="PLN02_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI51333.1"
                     /translation="MNDFDYLDLDGKLLRLLISVHEEGSVTRAAQRLGVTQSAVSHML
                     DKLRGIVGDPLFVKSGRSVVATAQAGALVAEARELLDRLHQFGSKAGFTPASLERTLT
                     IAANDLQRDLLLPALFGLLKEEAPGISLRVIPSNIPSAELLRSDRCQLAISPRPPEGA
                     DILQRRLFNDSYAVFFDPAVRPAPADLADYLAAEHITVVYEPQRNLDIDDHLQRRGIQ
                     RRFAVSVASFGGIRAFLKGSNYLATLPHRLGGNQLAGFARTATPFAGPEMPMYAIWHQ
                     RYQHDPMMRWLLDKLYRVSQEIEAAA"
     gene            2647..3003
                     /locus_tag="PLN02_03085"
     CDS             2647..3003
                     /locus_tag="PLN02_03085"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HOI51334.1"
                     /translation="MSIRPPKRTIPGAPIFDHEAARRGYALNKMCYSFNSKANRDEFV
                     SDEAAYCRKFGLSQEQTQAILERDVLSLIRVGGGVYFLGKLAGGVLGLDMQDIGALQT
                     GMSKEDFKAKLVAAGN"
     gene            3007..3843
                     /locus_tag="PLN02_03090"
     CDS             3007..3843
                     /locus_tag="PLN02_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HOI51335.1"
                     /translation="MAKIVGIMTTSHVPALGKAIHLNLQQDPYWKPFFDGFPAIREWL
                     DRVKPDIVVSCYNDHGLNFFLDQQPTFAIGTAPEYVSEDEGWGIPTMPSFPGAEDLSW
                     HIRDSLVRDDFDMVTCQEMLVDHAFTLPMALLWPEQKWPVKSVPVCVNTVLFPLPSAR
                     RCYRLGEAIGRAIAAFPEDAKVLVIGSGGLSHQLEGERAGFINKEFDLKFMDSMVNDP
                     TWATQFSDVDLVEQTGNEGVELLNWLVARGTVPGAVRVVHSNFHIPISNTAAATMLFE
                     PV"
     gene            4006..4347
                     /locus_tag="PLN02_03095"
     CDS             4006..4347
                     /locus_tag="PLN02_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51336.1"
                     /translation="MEKTLPEGHPDLQRIVQAAHADVPAADAKPAGNSLAGALAAALG
                     RQPGDAEDRENIEFLVQVLAVNRAEGNIDDAAFAALLERIATRAGRAALVASFTAELE
                     AEHGYADDPPA"
     gene            complement(4360..>4635)
                     /locus_tag="PLN02_03100"
     CDS             complement(4360..>4635)
                     /locus_tag="PLN02_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOI51337.1"
                     /translation="EEIRDKLIYYPTVTREPFRNEGRQTDLILSGKLFADLGIAPLDP
                     ATDRGMICGSPAMLREISAMLDGFGFHISKHIGEMGDYVIERAFVEQ"
BASE COUNT          711 a         1563 c         1541 g          820 t
ORIGIN      
        1 ccttcgtcgc cttcagtccg ctggcgcgcg gcttcctcgg cggcgagttg agcgacccgg
       61 cggcgcagtt gccgcccggc gacctgcgcc tggcgatgcc gcgtttcgcg cccgacaact
      121 acgcgcgcaa ccggcgcctg ctcgacggct atcgcgccat cgccgacgaa gccggctgca
      181 gtctggcgca actggcgttg gcctgggtcc tgcaccagca gccgcatacg ctgatcattc
      241 ccggtacccg ttcgccggcg catctcgccg acaacctcgg cgccgcccgg gtcagcctga
      301 gcgccgagct gatcgcccgc ctcgacgcac tgatcaaccg gcataccgtc agcggtccgc
      361 gctacaacgc ggcgacgctg gccgaaatcg acaccgagcg cttcccggaa tgagcgcggc
      421 gggcctcgcc agccgtattg tcgaccgcgc gccagcgctc ggccagccga atttccggcg
      481 ctatttctac ggccagatct gttccgtcct cggtaccttc atccaggccg tcgccatgtc
      541 ctggctggtg taccggatca gcggttcgcc ggtcctgctc gggctgaccg ccttcctgtc
      601 gcaggcaccg caactggtgg tggcgccgct cgccggtgtg atgatcgatc gctacgaccg
      661 gcgccggctg atgatcggcc tgcaatgcgt gtttgccgcg caggccctgg tcctggccgg
      721 gctggtcttc ttcgacgcgg tcgccgtctg gcacctgctg ctgctcgccg gcctgctcgg
      781 catcctcaac agtttcgacc tgccgacccg gcagagcctg ctgctctatc tggtcgacga
      841 ccggacgacg ctgcccaacg ccgtcgcgct caatgccgcg gtggtgcaca gcggacgcct
      901 gatcggtccg ccggtcgccg ggctgctcct ggccgtcgcc cccgaaagtc tgtgtttcct
      961 gatcaacgcg ctgtcctacc tgggtccgat ctcggcggtg ctcgccatcc gcgccgaaat
     1021 tccccggcag cacggcctga agaccgccag cgcgctggtc gaggtcgccg ccttcgtccg
     1081 tagcaccgtg gacgccaggg tcgccctgct ggcggtggcg atggtcaatg tcaccgcttc
     1141 ctcctacgtc gtgctgatgc cggttttcgc caaggaagta ttcgccggca gcgcccagtc
     1201 gctgggctgg ctgctcggca tgggcggcgc cggcgcgctg gtcgcctcgc tctatctgtc
     1261 gacccgggca aggagcgaaa tccggccgga tttcgcgatc ggcagttgcg cggtcgccgc
     1321 cgtcggcctg ctcggttttg cgctggccgg ctacgtcggc agcctggcgc tggcgctgcc
     1381 catggtctgc ctgatcggcg ccggcatcgg cgccaccaac atcgccgcca atacgcgctt
     1441 gcagcaactc gccccggaag gactgcgcgg ccgggtgatc tcgatcttcg gttcgacccg
     1501 cttcggcttc gatgcgctgg gcggtttgct cgccggcggg ctggcctcgt tgctcggtgt
     1561 cctgccggtc gtcggcgcgc tcggtctgct cctgctgccc ggcagtctgg cgttgctcag
     1621 gcggctgcct cgatctcctg gctgacccgg tacagcttgt cgagcagcca gcgcatcatc
     1681 gggtcgtgct ggtagcgctg gtgccagatc gcgtacatcg gcatctccgg gccggcgaag
     1741 ggcgtcgcgg tgcgggcgaa accggccagt tggttgccgc ccaggcgatg cggcagggtc
     1801 gccaggtagt tcgaaccctt gaggaaggcg cggatgccgc cgaagctggc gacgctgacg
     1861 gcaaagcggc gctggatgcc gcgccgctgc agatggtcgt cgatgtcgag gttgcgttgc
     1921 ggctcgtaga ccacggtgat gtgttcggcc gccaggtagt cggcgaggtc ggcgggggcc
     1981 gggcgtactg ccgggtcgaa gaagaccgcg taactgtcgt tgaacagccg gcgctgcagg
     2041 atgtcggcgc cttccggcgg gcgcggcgag atcgccagtt ggcagcggtc cgagcgcagc
     2101 agttcggcgc tcgggatatt ggacggaatg acgcgcaggc tgattcccgg cgcctcttcc
     2161 ttgagcagcc cgaacagcgc cggcagcagc aagtcgcgct gcaggtcgtt ggcggcgatg
     2221 gtcagtgtcc gttcgaggct ggccggggtg aagccggcct tgctgccgaa ctggtgcagg
     2281 cggtcgagca gttcgcgcgc ctcggcgacc agcgcgccgg cctgggcggt ggcgaccacc
     2341 gagcggccgg acttgacgaa cagcgggtcg ccgacgatgc cgcgcagctt gtcgagcatg
     2401 tggctgaccg ccgactgggt gacgcccagg cgctgcgcgg cgcgggttac cgagccttct
     2461 tcatggacgg agatgagcag gcgcagcagt tttccgtcga ggtccaaata atcgaaatcg
     2521 ttcatgaatt gcattataag caatctgttt attttgttgt tctctggatg gagaatccct
     2581 ctcgggctaa gggttatcgc agtccctcag caagcagcga ttcacaacac ttacaggaga
     2641 cgcaacatga gcattcgtcc gccgaagcga acgattcccg gcgcaccgat cttcgatcac
     2701 gaggccgccc ggcgcggtta tgcgctgaac aagatgtgct attcgttcaa cagcaaggcc
     2761 aaccgcgacg agttcgtcag cgacgaggcc gcctattgcc gcaagttcgg cctcagccag
     2821 gaacagaccc aggccatcct cgagcgcgac gtgctgtcgc tgatccgcgt cggcggcggc
     2881 gtctatttcc tcggcaagct ggccggcggc gtcctgggac tcgacatgca ggacatcggt
     2941 gccctgcaga cggggatgag caaggaagat ttcaaggcca aactggtcgc ggcggggaac
     3001 tgaatcatgg caaaaatcgt aggcatcatg accacttcgc acgtgccggc actgggcaag
     3061 gcgattcatc tcaacctcca gcaggacccg tactggaagc ccttcttcga cggcttcccg
     3121 gcgatccgcg aatggctcga ccgggtcaag ccggacatcg tcgtttcctg ctacaacgac
     3181 cacggcctga atttcttcct cgaccagcag ccgaccttcg ccatcggcac cgcgccggaa
     3241 tacgtcagcg aggacgaagg ctggggcatt ccgacgatgc cgagcttccc gggcgccgaa
     3301 gacctttcct ggcacatccg cgacagcctg gtccgcgacg atttcgacat ggtcacctgc
     3361 caggaaatgc tggtcgacca tgccttcacg ctgcccatgg cgctgctctg gccggaacag
     3421 aagtggccgg tgaagagcgt cccggtctgc gtcaataccg tgctcttccc gctgccgtcg
     3481 gcgcgtcgct gctaccggct gggcgaggcg atcggccgcg ccatcgcggc tttcccggaa
     3541 gacgccaagg tcctggtgat cggctccggc ggcttgtcgc accaacttga aggcgagcgt
     3601 gccggcttca tcaacaagga attcgacctc aagttcatgg acagcatggt caacgacccg
     3661 acctgggcca cccagttctc cgacgtcgac ctggtcgagc agaccggcaa cgaaggcgtc
     3721 gagctgctca actggctggt cgcccgcggc accgtgccgg gcgcggtgcg ggtggtgcat
     3781 tccaacttcc acatcccgat ctcgaatacc gcggcggcga ccatgctgtt cgagccggtc
     3841 tgagacaggt cttagcctaa agagagaagg gcgggtccgg gtggcccgcc cttcgtcatt
     3901 tccggcacaa tgacggcgcc ggcgccgaag ccgcccgcgc gctgcgggcg cagccactga
     3961 cgcttcacgt cgccaactgt ctgattgcaa ccgaaggcgc attccatgga aaagacccta
     4021 cccgaaggac atcccgacct gcagcgcatc gtccaggcgg cgcacgccga cgtgccggcg
     4081 gccgatgcca aaccggccgg caattcgctg gccggcgcgc tcgccgccgc gctgggccgc
     4141 cagccgggcg atgccgagga ccgcgagaac atcgaatttc tggtccaggt gctggcggtc
     4201 aatcgcgccg aaggcaacat cgacgacgcg gctttcgccg cgctgctcga acggattgcg
     4261 acgcgcgccg ggcgcgccgc gctggtcgcc agcttcaccg ccgagctgga ggctgagcac
     4321 ggctacgccg acgacccgcc ggcctgaggc cggccccgct tactgctcga cgaaggcgcg
     4381 ctcgatcacg tagtcgccca tctcgccgat gtgcttcgag atgtggaagc cgaagccgtc
     4441 gagcatggcc gagatttccc tgagcatcgc cgggctgccg cagatcatgc cgcggtcggt
     4501 ggccgggtca agcggtgcga tgccgaggtc ggcgaacagc ttgccggaga ggatcaggtc
     4561 ggtctgccgg ccttcgttgc ggaagggctc gcgggtcacg gtcgggtagt agatcagctt
     4621 gtcgcggatt tcctc
//
LOCUS       DAOSDX010000096         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6534_length_4632_cov_3.421018, whole genome shotgun sequence.
ACCESSION   DAOSDX010000096 DAOSDX010000000
VERSION     DAOSDX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6534_length_4632_cov_3.421018"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..742)
                     /locus_tag="PLN02_03105"
     CDS             complement(170..742)
                     /locus_tag="PLN02_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51338.1"
                     /translation="MSELYEKLYFHEIEIRDKLVERLQVPLALIVSLMGALVYMLQNT
                     ALESPLPILVPYFFVLAVPAILLIATAVTFFLSYYNYSYGLIPTAEQLEAYRQQLLAT
                     YTEFDNGQVLAEKYHREALMNAFVECSTINAATNAKRGGYIHTTNTLTLISAIFLLIA
                     FVFFYLGGLDKNGLDTPKQIVISKPIELRR"
     gene            complement(1310..2374)
                     /gene="fba"
                     /locus_tag="PLN02_03110"
     CDS             complement(1310..2374)
                     /gene="fba"
                     /locus_tag="PLN02_03110"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929444.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HOI51339.1"
                     /translation="MPLVSLRQLLDHAAEHDYGLPAFNVNNMEQVWAIMEAASQLDAP
                     VIMQASAGARKYAGEPFLRHQILAALEAYPHIPIVMHQDHGQSPAVCMAAIKSGFSSV
                     MMDGSLEADGKTVASFEYNVEVTRKVTELAHSIGVSVEGELGVLGSLETMKGDKEDGH
                     GAEGTMTRDQLLTDVEQAADFVRQTNCDALAIAIGTSHGAYKFTKKPTGDILAIDRIA
                     EIHARIPNTHLVMHGSSSVPQELLAEIREFGGDMKETYGVPVEEIVKGIKHGVRKVNI
                     DTDIRLAMTGAIRRYLAENPGKFDPRDYLKPAREAAKKICLARFEAFGCAGQASTIKP
                     VSLDKMADRYAKGELNQIVK"
     gene            complement(2420..>2885)
                     /locus_tag="PLN02_03115"
     CDS             complement(2420..>2885)
                     /locus_tag="PLN02_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOI51340.1"
                     /translation="MARICVEAERSAEVTLDREFLDRVFTRIDQSIAMAAIWTAHHLK
                     VKAIAALTQSGSTALWMSRLNCGVPIYALTPDAAAIGRMALYREVRPLLMQQQHTERD
                     QLLAEAEALLIERGIVDRGDLIVLTIGEPIGSIGGTNTLKIVRVGEHLPTRP"
     assembly_gap    2886..2895
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2896..3870)
                     /gene="pyk"
                     /locus_tag="PLN02_03120"
     CDS             complement(<2896..3870)
                     /gene="pyk"
                     /locus_tag="PLN02_03120"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOI51341.1"
                     /translation="MSRHTKIVATLGPASSSPEVLERMVQAGIDVVRMNFSHGTAADH
                     KARAEGIRAAAAKHGRVVGILGDLQGPKIRVGKFESSPITLVADAHFILDAACPLGNQ
                     ERVGLDYKDLPKDVVPGDILLLDDGRLKLTVEAVRGTEIHTRVLVGGELSNNKGINRQ
                     GGGLTAPALTAKDMDDIKTAAEIGVDFVAVSFPKSAADMYMARQLLRAAGSEAVLIAK
                     IERVEAVDNMAEILDASDGVMVARGDLAVEVGDAAVPALQKKMIRMARDKNKLAITAT
                     QMMESMIHSPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVETVES"
     gene            complement(3919..>4632)
                     /locus_tag="PLN02_03125"
     CDS             complement(3919..>4632)
                     /locus_tag="PLN02_03125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI51342.1"
                     /translation="WDIEAVPDDADSQAAFGNRTQACRHLCNQMAEIADPEIWSASVL
                     AFGLESESAISERFGAAVMQDIGQRFARVIRAKLGPRDLIAHLGGSTYVVASPGTSPA
                     TATAFAQRVCSALGESRIRIAETTVQVAVSAGLAALPAERGKSAAQLIEMAVSRLELA
                     RQTPGGHVISSDPPADDPFARIQALLRQENTREQLGETGQQLLPILRLLEQQFHFGLP
                     LAQMEEQLRKYASHQPDPE"
BASE COUNT          782 a         1275 c         1568 g          997 t
ORIGIN      
        1 gaaagttaga tagagcgcta ttttttcggt ggtggcgttg gtgtcttatt ctgcggcgga
       61 ggcgtgttgc tattgttcga tcggactgat ggcctgtcca cgtggctctt taccatacgg
      121 attggtggcg gcgggggcgg cgggggcgga tttttcgtat ttgacatgac tatctcctaa
      181 gttcaattgg cttactgatc acgatttgtt tcggtgtatc cagaccgttc ttgtcgagtc
      241 ctcccaaata gaaaaacacg aacgctatta gaaggaatat ggcagagata agcgtcagcg
      301 tattagttgt gtggatgtag ccacctcgtt tggcatttgt agctgcattg atcgtggagc
      361 actcaacgaa ggcattcatt agcgcctcgc ggtgatattt ttccgccaat acctgaccat
      421 tgtcgaattc ggtgtacgtc gcaagcaact gttgcctgta tgcctctagc tgttcggcgg
      481 tcgggataag cccataggaa tagttgtagt aagagagaaa aaatgtgaca gcagttgcaa
      541 tgagcaagat tgccggaacg gcaagtacaa agaagtaagg aacaaggatt ggaagcggcg
      601 actcaagtgc ggtgttttgc aacatgtaga ccagggcgcc catgagcgac actatgagcg
      661 cgagaggtac ttgaagacgc tccacaagct tgtcacgaat ttctatctcg tgaaaataaa
      721 gcttctcgta aagctcagac atgctgtcgt tctttctcta acgttaaatg tggaacctaa
      781 ccaacggaaa acaatccgtc ttgtaggctg cataaaaaac cagattcatg ttgtttcaac
      841 tacttggcag attttagggg ggcgcctcaa gagcgccttg gatcgttcgc gtccaccgtc
      901 ttcggcagta acttgatgaa tacccgccat gcgggcagtt gatatccaat ccgatacgga
      961 accccaacac gccgagcatg aatttctgcc tttcattcct tctgtggctg gttgcatgca
     1021 gaggcaaaaa gattcgggcg attgagaatg gtacggacgc cggcgaacgg cctctgggtt
     1081 tttgaatgac atgaagatta tcccgagtgg ggctccttat caatgactga tcatatggac
     1141 gcagccaacg tcggctatgc acgtgttttc ttgaaggccc gctgctggcc gggctgagac
     1201 tttgccatga tcgggcagcg tgacgcagtt aagtcggttc cggcgtccag cgaccaaaca
     1261 aaacagccac ccgaaggtgg ctgtttgtcg tggggcctga aaagccggct tacttgacga
     1321 tctggttcag ctcgcccttg gcgtagcggt cggccatctt gtccagcgat accggcttga
     1381 tcgtgctcgc ctggccggcg cagccgaagg cttcgaaacg ggcgaggcag atcttcttgg
     1441 cggcttcgcg ggccggcttg aggtagtcgc gcgggtcgaa cttgcccggg ttttcggcca
     1501 ggtagcggcg gatggcgccg gtcatcgcga ggcggatgtc ggtgtcgatg ttgaccttgc
     1561 gcacgccgtg cttgatgccc ttgacgattt cctcgaccgg cacgccgtag gtttccttca
     1621 tgtcgccgcc gaattcgcgg atttcggcga gcagttcctg cggcacgctg gaggagccgt
     1681 gcatcaccag gtgggtgttc gggatgcggg cgtggatttc ggcgatgcgg tcgatggcga
     1741 ggatgtcgcc ggtcggcttc ttggtgaact tgtaggcgcc gtggctggtg ccgatggcga
     1801 tggcgagcgc gtcgcagttg gtctggcgga cgaagtcggc ggcctgctcg acgtcggtca
     1861 ggagctggtc gcgggtcatc gtgccttcgg cgccgtggcc gtcttccttg tcgcccttca
     1921 tggtttccag cgagccgagc acgcccagtt cgccttcgac cgagacgccg atgctgtgcg
     1981 ccagttcggt gaccttgcgg gtgacttcga cgttgtattc gaagctggcg acggtcttgc
     2041 cgtcggcttc cagcgagccg tccatcatca ccgacgagaa gccggacttg atcgccgcca
     2101 tgcagacggc cgggctctgg ccgtggtcct ggtgcatgac gatggggatg tgcgggtagg
     2161 cttcgagggc ggcgaggatc tggtggcgca ggaagggttc gccggcgtat ttgcgggcac
     2221 cggccgaggc ctgcatgatg accggcgcat cgagctggct ggcggcttcc atgatggccc
     2281 agacctgctc catgttattg acgttgaagg cgggcagacc gtagtcgtgt tcggcggcat
     2341 ggtcgagcag ttggcgcagg gaaacgagcg gcatgggaac tcctggatag ttgggtcgct
     2401 gaattaaccg gctattttat tacggacggg tcggcagatg ctcgccgacg cgcacgatct
     2461 tcagcgtgtt ggtaccgccg atgctgccga tcggttcgcc gatggtgagc acgatcaggt
     2521 cgccgcggtc gacgatgccg cgttcgatga gcagggcctc ggcttcggcc agcaactggt
     2581 cgcgctcggt atgctgttgc tgcatgagca gcggacgcac ttcgcggtac agcgccatgc
     2641 ggccaatcgc ggcggcatcc ggggtcagcg cgtagatcgg cacgccgcag ttgagccggc
     2701 tcatccacag cgcggtcgac ccggactggg tcagcgcggc gatcgccttg accttgaggt
     2761 ggtgcgcggt ccagatggcg gccatggcaa tcgactggtc gatgcgggtg aacacgcggt
     2821 cgaggaactc gcggtcgagc gtgacttcgg ccgagcgctc ggcttcgacg cagatgcgtg
     2881 ccatcnnnnn nnnnncgatt cgacggtctc gaccgggtac ttgccggcgg cggtttcggc
     2941 cgacagcatc accgcgtcgg taccgtcgag gacggcgttg gcgacatcgg aaacttcggc
     3001 gcgggttggc accggcgaat ggatcatcga ttccatcatc tgcgtcgcgg tgatcgccag
     3061 cttgttcttg tcgcgcgcca tgcggatcat cttcttctgc agcgccggca cggcggcgtc
     3121 gccgacttcg accgcgaggt cgccacgggc gaccatgacg ccgtcggagg catcaaggat
     3181 ctcggccatg ttgtcgaccg cttcgacgcg ttcgatcttg gcgatcagta ccgcctcgct
     3241 gccggcggcg cgcagcaact ggcgggccat gtacatgtcg gcggcgctct tggggaagga
     3301 gacggcgacg aaatcgacgc cgatctcggc ggcggtcttg atgtcgtcca tgtccttggc
     3361 ggtcagcgcc ggtgcggtca ggccgccgcc ctggcggttg atccccttgt tgttcgacag
     3421 ctcaccgccg accagcacgc gggtgtggat ttcggtgccg cgcacggctt cgaccgtgag
     3481 cttgagacgg ccgtcgtcga gcagcaggat gtcgccgggg acgacgtcct tcggcaggtc
     3541 cttgtagtcg aggccgaccc gctcctgatt gccgagcggg caggcggcgt cgaggatgaa
     3601 atgggcatcg gcgaccagcg tgatcgggct gctctcgaat tttccgacac ggatcttcgg
     3661 cccctgcagg tcgccgagga tgccgacgac gcggccgtgt ttggcggcgg cggcacggat
     3721 gccttcggcc ctggccttgt ggtcggcggc ggtgccgtgc gagaagttca tgcggacgac
     3781 atcgatgccg gcctgcacca tgcgctccag gacttccgga gacgacgagg cggggccgag
     3841 ggtggcgacg atcttggtat ggcgggacat tgtctcttcc tgtctatagg tgtggttatt
     3901 ctgtcttgct acgacgattc attctgggtc aggctgatga ctggcgtatt tccggagctg
     3961 ttcctccatt tgtgccaggg ggaggccgaa atggaactgc tgttcgagca ggcgcaggat
     4021 gggcagcagt tgctggccgg tttcgcccag ttgctcgcgg gtgttttcct ggcgcagcag
     4081 ggcctggatg cgggcgaagg ggtcgtcggc cggagggtcg ctgctgatga catggccgcc
     4141 aggggtctgg cgggcgagtt cgaggcggct gaccgccatt tcgatcaact gcgcagccga
     4201 tttgccgcgt tccgccggca gggcggcgag accggcgctg acggcgacct gtaccgttgt
     4261 ttcggcgatc cggatgcggc tctcgccgag ggcgctgcac acgcgttggg cgaaggcggt
     4321 tgccgtcgcc gggctggtgc cgggcgaggc gacgacatag gtcgagccgc cgaggtgggc
     4381 aatcaggtcg cgcgggccga gcttggcgcg gatcacccgc gcgaaacgct ggccgatgtc
     4441 ctgcatcacc gccgcgccga agcgttcgct gatcgccgat tcgctctcca gcccgaaagc
     4501 cagcacgctg gccgaccaga tttccggatc ggcaatttcc gccatctggt tgcatagatg
     4561 gcggcaggcc tgggtgcggt tgccgaaggc tgcctggctg tcggcgtcgt cggggacggc
     4621 ttcgatatcc ca
//
LOCUS       DAOSDX010000097         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6561_length_4619_cov_3.221516, whole genome shotgun sequence.
ACCESSION   DAOSDX010000097 DAOSDX010000000
VERSION     DAOSDX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6561_length_4619_cov_3.221516"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..739
                     /locus_tag="PLN02_03130"
     CDS             95..739
                     /locus_tag="PLN02_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOI51343.1"
                     /translation="MPVQDGKRLSFKLVYYGPAQSGKTTNLLALHEQLAPEQKGEMMS
                     METQDDRTLFFDLLPLGFHAPSGLSIRCRLFTVPGQVAHDGTRKAVLSRADGVVFVAD
                     LQRNQETNNAEAFRSLSENCARVGIDLERLPLVVQFNKSDLPSAIPQTEIRERWQSAP
                     WPLHFASALHGEGVRETFASLLGALYRRQDEACGLSREHGLNEAAFVAGLMGTP"
     gene            736..1944
                     /locus_tag="PLN02_03135"
     CDS             736..1944
                     /locus_tag="PLN02_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51344.1"
                     /translation="MSGAHFDHDWQLGELIDSHELPELAAALARLLGGDLALADGSGR
                     IVWGTASPGARRQAVVIELDPVATLCSASASADALTAAGELLHDLLHTRIRYRMASAL
                     HLEVVAEDFASLKREHARLAESEARYRQLASELDARVKTQLTELEERQQMLYAAERLA
                     SIGQLAAGMAHEINNPLGFARSNLGSFRGYLQKFAALRDDLDHARAAWQALDLDFLLA
                     DGDELLDETLAGLERIAHIVADLKAFSNVDRSSEAYADLNTCLREAAAVIEAQLPAGI
                     GLRLDLLPLPPIVCLPGHLKQAFYNILHNALQAIIDSGRPGTIILSSAAEAGRLTVRI
                     RDNGIGMRPEQLAHAFEPFYTTRDVGHGVGLGLATARSIVVAHGGKITLDSAVDAGTT
                     VTLSFPIPDD"
     gene            1937..4177
                     /locus_tag="PLN02_03140"
     CDS             1937..4177
                     /locus_tag="PLN02_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI51345.1"
                     /translation="MTDYSNLFTGKQTVAQAPATHLTPPRYRLLLVDDEPGILRALVR
                     VFRQENYEVISAGSGEEGLQRLAEGSFHLVISDFLMPGMDGARFLREVKEKSPDTIRI
                     MLTGHANTDAVMGAINDGAVYKFILKPWNDDDLRITVALALEQHDLISRNRVLKADNE
                     KKQKEISALSRLAASHRSRLGIMLHKHQLLSAAQLQELSQLQERRKEALIVLLLEKDW
                     VPERRIRELLRREMLIDEVQLPEFQVDSALTELIPHSFCARQLVVPLKLQGRRLLLAM
                     ADPLDEGLIDEVRFAAGLDIDVVTADIAAIKRKIEELYGTDTSDFRELETLISTSDPY
                     EGIEIVIDDDAQASLEELLQETTAPPAIRLANAIILEAIRLGSSDIHIQPRTKSVVVR
                     YRIDGVLADKISIPHQLHQSLVSRLKIMAELDISERRRPQDGRITVKTPLRLVDLRIS
                     TLPTINGEKVVMRILDRNAAVHSLEHLGFSTVDLPRVADMVARPQGIILATGPTGSGK
                     TTTLYSLLQHDATPGKNYVTIEDPVEYYLDMAGQVLIREKIGLTFPMVLRALLRQDPD
                     VILLGEIRDFETAEVAFHAALTGHLVYSTLHTNSAIATIARLFDLGLKPYVIATALEG
                     IIAQRLVRRVCPKCSKVGAADPLLRARLGGAFADIRETRRGAGCGECHGSGYKGRVGI
                     YEVLTLNDELRDRIGSSASILEIRRLVRHQGLRSIAHHAAERVAAGITTFEEILRVLG
                     PQAGED"
     gene            4180..>4619
                     /locus_tag="PLN02_03145"
     CDS             4180..>4619
                     /locus_tag="PLN02_03145"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOI51346.1"
                     /translation="MDAFVWDQRFTTGEPSVDAEHRELVRLINQIIESQSKYANTATI
                     ATILDQLVAYAALHFANEEALMAECGCDPRFIAKHTAVHTGFADQVIKLKSMPAGSIT
                     LPNLVNYLTAWLAQHILGMDQSMARQINAIRAGSSAAAAYAAEDA"
BASE COUNT          852 a         1695 c         1399 g          673 t
ORIGIN      
        1 gcggaacgtc tggaacgcct ggaacccggc atcaccaagg tcaactgggg accggacggc
       61 tcggtgctgc tcgacgaggc aggggccgac tgaaatgcct gtccaggacg gcaagcgcct
      121 gagcttcaag ctggtctatt acggaccggc gcagagcggc aagacgacca acctgctggc
      181 gctgcacgaa caactggccc ccgagcagaa gggcgaaatg atgagcatgg agacgcagga
      241 cgaccgcacc ctgttcttcg acctgctgcc gctcggtttc cacgccccct ccgggctgtc
      301 gatccgctgc cgcttattca ccgtacccgg ccaggtcgcc cacgacggca cccgcaaagc
      361 ggtgctgtcg cgcgccgacg gcgtcgtctt cgtcgccgat ctccagcgca accaggaaac
      421 caacaatgcc gaagccttcc gcagcctgag cgagaactgt gcgcgcgtcg gcatcgacct
      481 cgaacgcctg ccgctggtcg tccagttcaa caagagcgac ctgccgagcg ccatcccgca
      541 gacggaaatc cgcgagcgct ggcagtcggc gccgtggccg ctgcatttcg cctcggcgct
      601 gcatggcgaa ggcgtgcgcg agaccttcgc cagcctgctc ggcgccctct accggcgcca
      661 ggacgaggcc tgcggcctga gccgcgaaca tggcctgaac gaggccgcct tcgtcgccgg
      721 attgatgggg acgccatgag cggggcgcat ttcgaccatg actggcagct cggcgagctg
      781 atcgacagcc acgaactacc cgaactggcc gccgccctcg cccgcctgct cggcggcgat
      841 ctggcgctgg ccgacggcag cggccgcatc gtctggggca cggccagccc aggcgcccgc
      901 cggcaagcgg tggtgatcga actcgacccc gtcgccaccc tgtgcagcgc cagcgccagc
      961 gccgatgccc tgacggcggc cggcgagctg ctgcacgacc tgctgcacac ccgtatccgc
     1021 taccggatgg cgtcggcgct gcatctcgaa gtggtggccg aggatttcgc cagcctgaag
     1081 cgcgaacatg cgcgcctcgc cgaatcggaa gcccgctacc gccaactggc gagcgagctc
     1141 gacgcccggg tcaagaccca gcttaccgaa ctggaagagc gccagcagat gctctacgca
     1201 gcggaaaggc tcgcctcgat tggccaactg gcggccggca tggcacacga aatcaacaac
     1261 ccgctgggct tcgcgcgcag caacctgggc agcttccgcg gctatttgca gaaattcgcc
     1321 gccctgcgcg acgatctcga ccacgcccgc gccgcctggc aggcgctcga cctcgatttc
     1381 ctgctcgccg acggcgacga actgctcgat gaaacgctcg ccggactcga acgcatcgcc
     1441 cacatcgtcg ccgacctcaa ggccttttcc aacgtcgacc gcagcagcga agcctacgcc
     1501 gacctcaaca cctgcctgcg cgaagccgcc gccgtgatcg aggcccaatt gccggccggc
     1561 atcggcctgc gcctcgacct gctgcccttg ccgcccatcg tctgcctgcc cggccatctc
     1621 aagcaggcct tctacaacat tctgcacaac gccctccagg ccatcatcga tagcggccgg
     1681 ccgggcacga tcatcctctc ttccgccgcc gaagccggac ggctgaccgt gcgcatccgc
     1741 gacaacggca tcggcatgcg cccggaacaa ctcgcccacg ccttcgaacc cttctacacg
     1801 acgcgcgacg tcggccatgg tgtcggtctc gggctggcca ccgcacgcag catcgtcgtc
     1861 gcccacggcg gcaagatcac gctcgacagc gccgtggacg ccggtaccac cgtcaccctc
     1921 agcttcccga tcccggatga ctgactacag caacctgttc accggcaagc agacggtcgc
     1981 ccaggccccg gccacgcatc tgacgccccc ccgttaccgg ctgctcctgg tcgacgacga
     2041 gccgggcatc ctgcgcgcgc tggtccgcgt cttccgccag gaaaactacg aggtgatcag
     2101 cgccggcagc ggcgaggaag gcctgcagcg cctcgccgaa ggcagcttcc acctggtgat
     2161 cagcgacttc ctgatgcccg gcatggacgg cgcgcgcttc ctgcgcgagg tcaaggagaa
     2221 gtcgcccgac accatccgca tcatgctcac cggccacgcc aacaccgacg cggtgatggg
     2281 ggcgatcaac gacggcgcgg tctacaagtt catcctcaag ccgtggaacg acgacgacct
     2341 gcgcatcacc gtcgccctgg ccctcgaaca gcacgacctg atcagccgca accgggtgct
     2401 gaaggcggac aacgagaaga agcagaagga aatctccgct ctgtcgcggc tggcggcgtc
     2461 gcaccgcagc cgactgggca tcatgctgca caagcaccag ttgctgagcg ccgcccagtt
     2521 gcaggagctc agccagctcc aggaaaggcg caaggaggcg ctgatcgtcc tgttgctgga
     2581 aaaggactgg gtgccggaac ggcgcatccg cgagttgctg cgccgcgaaa tgctgatcga
     2641 cgaagtccag ttaccggagt tccaggtcga cagcgcactg accgaactga tcccgcacag
     2701 cttctgcgcc cgccaactgg tggtgccgct caagctgcag ggccgccgcc tgctgctggc
     2761 gatggccgat ccgctcgacg aggggctgat cgacgaggtg cgttttgccg ccggcctcga
     2821 catcgacgtc gtcaccgccg acatcgcggc gatcaagcgc aagatcgagg aactctacgg
     2881 caccgacacc agcgatttcc gggaactgga aacgctgatc agcaccagcg acccctacga
     2941 gggcatcgag atcgtcatcg acgacgacgc gcaggccagc ctcgaggagt tgctgcagga
     3001 aacgacagcg ccaccggcga tccggctggc caatgcgatc atcctcgaag cgatccgcct
     3061 cggcagttcg gacatccaca tccagccgcg caccaagagc gtcgtcgtcc gctaccggat
     3121 cgacggggtg ctggccgaca agatcagcat tccccaccag ctgcaccagt cgctggtgtc
     3181 gcgcctgaag atcatggccg agctcgacat ttccgagcgc cggcggccgc aggacggacg
     3241 gatcacggtg aagacgccgc tgcgcctcgt cgacctgcgc atctcgacgc tgccgacgat
     3301 caacggcgag aaggtggtga tgcgcatcct cgaccgcaac gccgccgtgc actcgcttga
     3361 gcacctcggc ttttccaccg tcgatctgcc gcgggtcgcc gacatggtgg cccgaccgca
     3421 gggcatcatc ctcgccaccg gccccaccgg cagcggcaag acgacgacgc tgtactcgct
     3481 gctgcagcac gacgcgacgc cgggcaagaa ctacgtgacg atcgaggacc cggtcgagta
     3541 ctacctcgac atggccggcc aggtgctgat ccgggagaag attggcctga ccttcccgat
     3601 ggtgctgcgc gccctgctcc gccaggaccc ggacgtcatc ctgctcggcg aaatccgcga
     3661 tttcgagacc gccgaggtcg ccttccatgc cgcgctgacc ggccacctcg tctattcgac
     3721 gctgcacacc aattcggcga tcgccaccat cgcccgcctg ttcgaccttg ggctcaagcc
     3781 ctacgtgatc gccaccgccc tcgaaggcat catcgcccag cgcctggtgc gccgcgtctg
     3841 cccgaagtgc agcaaagtcg gcgccgccga cccgctgctg cgcgctcgcc tcggcggtgc
     3901 cttcgccgac atccgcgaga cccgccgcgg cgcgggttgc ggcgaatgcc acggcagcgg
     3961 ctacaaggga cgggtcggca tctacgaggt actgacgctc aacgacgagt tgcgcgaccg
     4021 catcggcagc agcgccagca tcctggaaat ccgccgcctg gtccgccacc agggcctgcg
     4081 cagcatcgcc caccacgccg ccgagcgggt cgccgccggc atcaccactt tcgaagaaat
     4141 cctgcgcgtc ctcgggccgc aggccgggga ggattgacca tggacgcctt tgtctgggac
     4201 cagcgcttca ccaccggcga gccaagcgtc gacgccgagc accgggaact ggtccgcctg
     4261 atcaaccaga tcatcgaatc gcaatcgaaa tacgccaaca cggcgaccat tgccaccatc
     4321 ctcgatcagc tagttgccta tgccgccctg catttcgcca acgaagaggc gctgatggcc
     4381 gaatgcggct gcgatccccg cttcatcgcc aagcacaccg ccgtgcacac gggtttcgcc
     4441 gaccaggtga tcaagctgaa gagcatgccg gccggcagca tcaccctgcc caatctggtc
     4501 aattacctca ccgcctggct ggctcagcac atcctcggca tggaccagtc gatggctcgc
     4561 cagatcaacg ccatccgcgc cggcagcagc gccgctgccg cctatgccgc cgaggacgc
//
LOCUS       DAOSDX010000098         4610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6578_length_4610_cov_4.007245, whole genome shotgun sequence.
ACCESSION   DAOSDX010000098 DAOSDX010000000
VERSION     DAOSDX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6578_length_4610_cov_4.007245"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..2505
                     /locus_tag="PLN02_03150"
     CDS             79..2505
                     /locus_tag="PLN02_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOI51347.1"
                     /translation="MSARLLHIRGLVQGVGFRPYVWRLAGELGIAGWVRNDGDGVTLL
                     AAGARLDDFLARLPREIPPLARIDAIDSGLSDERPAPGFVIRDSVAGAVRTAIGPDAA
                     ICPDCISDLCDPTNRRWRYAFTTCTHCGPRHTVSAGIPYDRARTSLAAFPLCAPCAAE
                     YTDPADRRFHAETTCCPDCGPQLQLLDGNGADLAGGDDPIAATLALLRAGRIVAIKGL
                     GGFHLACDARNPDAVAELRRRKAREAKPFAVMGLNPAALAAFAEFGDGERALFADLAA
                     PIVLCRKGGEELAGVAPGLPWLGVMRPATPLHLLLWHEAAGRPAGTDWLGEENDLLLV
                     MTSANPQGEPLVTGNAEALLRLNGIADAFLMHDRDIVVRCDDSVVRAVFLAPTGPGDA
                     PAEPLPEKPAVDDDLPGAAPGAVPPRSPAAAGHPGFTPAFLRRARGYVPLPIPLAADG
                     PPVLALGAHLKNAVCVLRGREAFVSQHIGSLDNAATLAFLEETVAHLLAVLDVKPALI
                     AHDLHPDYASTALALRLAGELGVPTRAVGHHHAHVGAICAEQGVNDPLLGLALDGVGL
                     GPDGGAWGGELLRVDGADCRRLGHLRPLALPGGDRAAREPWRMAVAALHGAGLGERVA
                     GWLARTWPEREAGPLLTLLDRQLRCPPTSSLGRWFDAAAGLLGVRDVSRYEGQAAMEL
                     EGRAAAHGPLEPLAGGWRIAGGVLDFSPLLPALLDCRDAGAGAALFHATVAAGLAEWA
                     MLAVDREKLAKMAVGGGCAANAILLDALRRRLTAAGIEVLEARAVPAGDGGLALGQAW
                     IARQMHGDKA"
     gene            2502..2999
                     /gene="hybE"
                     /locus_tag="PLN02_03155"
     CDS             2502..2999
                     /gene="hybE"
                     /locus_tag="PLN02_03155"
                     /inference="COORDINATES: protein motif:HMM:NF023365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HOI51348.1"
                     /translation="MNDLASAAIHTDNPAPRVLARYRRWQAALNGGHPGLSVDIAGFR
                     RYRGDWIGAVVTPWFIHLLLLPGGGELWRELPVEQTLRVGFPAGDLDLISERSDDADL
                     PASFFVPILAPVNELADQDDALRTAMDALHLLFEPPAAAPTPTNPEPQPVDRRGFFRR
                     LAGRG"
     gene            3021..3269
                     /locus_tag="PLN02_03160"
     CDS             3021..3269
                     /locus_tag="PLN02_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOI51349.1"
                     /translation="MCLAIPALVVELRDGDNAVVDLAGVRKEISLSLVEGVAVGDYVI
                     VHVGFALNKLDPEEAEQTLKLFAEMGQLTADGDGLTLH"
     gene            3269..4396
                     /gene="hypD"
                     /locus_tag="PLN02_03165"
     CDS             3269..4396
                     /gene="hypD"
                     /locus_tag="PLN02_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOI51350.1"
                     /translation="MKYIDEFRDGDVAQKIAASIRAEADPARHYHFMEFCGGHTHAIS
                     RYGVSDLLPANVRMIHGPGCPVCVLPIGRVDQAIRLALDEGVTLCTYGDCLRVPASDS
                     LSLLKAKAKGGDIRMVYSSADAVTLAQKNPDKQVVFFAIGFETTTPPTAVAIKQAAAL
                     GLKNFSVLCCHVLTPSAISSILESPEVRQWGTVPLDGFVGPAHVSTIIGSRPYEFFAE
                     EYRKPVVIAGFEPLDVMQAIRMLIRQVNEGRAEVENEFSRAVDRDGNLKAQALVAEVF
                     EMRKHFEWRGLNVLPYSALRIRGAFAEFDAEKRFPVTYASVADHKACECGAILRGVKR
                     PQDCKIFGTVCTPENPVGSCMVSSEGACAAHYTYGRYKDVA"
BASE COUNT          679 a         1702 c         1505 g          724 t
ORIGIN      
        1 cgagtaccac accaaatcgc tgcccgacga cgagctgaag gcgcgcatcg ccgacctgga
       61 gcgacagctg gcggccaagt gagcgctcgc ctgctgcaca tccgcggcct cgtccagggc
      121 gtcggctttc gcccctacgt ctggcggctg gccggagaac tcggcatcgc cggctgggtg
      181 cgcaacgacg gcgacggcgt cacgctgctc gccgccggcg cccggctcga cgacttcctc
      241 gcccgcctgc cgcgcgagat cccgccgctc gcgcgcatcg acgccatcga cagcggcctc
      301 agcgacgaac gccccgcgcc cggcttcgtc atccgcgaca gcgtcgccgg cgcggtgcgc
      361 accgccatcg gcccggacgc agcgatctgc ccggactgca tctccgacct ctgcgaccca
      421 accaaccgcc gctggcgcta tgccttcacc acctgcaccc actgcggccc gcgccatacc
      481 gtcagcgccg gcattcccta cgaccgcgcc cgtaccagcc tggccgcttt tccgctgtgc
      541 gcgccgtgcg ccgccgaata caccgacccg gccgaccgcc gcttccacgc cgaaaccacc
      601 tgctgccccg actgcggacc gcagttgcaa ctgctcgacg gaaatggcgc cgatcttgcc
      661 ggcggtgacg atcccatcgc cgccacgctc gccctgctgc gcgccggccg catcgtcgcg
      721 atcaagggtc taggcggctt ccatctcgcc tgcgacgcgc gcaatcccga cgccgtcgcc
      781 gaactgcgcc ggcgcaaggc gcgcgaggcc aagcccttcg cggtaatggg cctcaacccc
      841 gccgcgctcg ccgctttcgc cgaattcggc gacggcgagc gggcgctgtt cgccgatctc
      901 gccgcgccca tcgtcctctg ccgcaagggc ggcgaggaac tggccggcgt cgcccccggc
      961 ctgccctggc tcggcgtcat gcgcccggcg acacccttgc atctgctgct ctggcacgaa
     1021 gccgccggcc gtcctgccgg caccgactgg ctcggcgagg aaaacgacct gctgctggtg
     1081 atgaccagcg ccaacccgca gggcgaaccg ctggttaccg gcaacgccga agccttgctc
     1141 cgcctgaacg gcatcgccga cgccttcctg atgcatgacc gcgacatcgt cgtccgctgc
     1201 gacgattcgg tggtgcgcgc ggtgtttctg gcaccgaccg gccctggcga cgcgccagcg
     1261 gagccgctgc ccgaaaaacc ggcggtcgac gacgatcttc ccggcgcagc gccaggcgcc
     1321 gtgccgcccc gctcaccggc cgcggcaggc catcctggct tcacgccagc cttcctgcgc
     1381 cgggcgcgcg gttacgtgcc gctgcccatt ccgctcgccg ccgacggccc gccggtactg
     1441 gcgctcggcg cccatctgaa gaacgccgtc tgcgtgctgc gcgggcgcga ggctttcgtc
     1501 tcgcagcaca tcggcagcct cgacaacgcg gcgacgctgg cttttctcga agaaaccgtc
     1561 gcccacctgc tcgccgtgct cgacgtcaaa ccggcgctga tcgcccacga cctgcacccg
     1621 gactacgcgt cgaccgcgct ggccctgcgt ctggccggcg aactcggcgt gccgacacgc
     1681 gccgtcggcc atcatcacgc gcatgttggc gcgatctgcg ccgaacaagg cgtcaacgac
     1741 ccgcttttag ggctggcgct cgacggcgtc ggcctcggcc cggatggcgg cgcctggggc
     1801 ggtgaactgc tgcgcgtcga cggcgccgac tgtcgccgcc tcggccatct gcgcccgctg
     1861 gccctgcccg gcggcgaccg tgcggcgcgc gagccatggc ggatggcggt tgccgcgctg
     1921 cacggcgccg gcctcggcga gcgcgtcgcc ggctggctgg cgcgcacctg gccggaacgc
     1981 gaggccggcc cgctgctgac gctgctcgac cggcaactgc gctgcccgcc gacctccagc
     2041 ctcggccgct ggttcgacgc cgccgccggc ctgctaggcg tgcgcgacgt ttcgcgctac
     2101 gaaggtcagg cggcgatgga actcgaaggc cgggcggcgg cgcacggacc gctcgaaccc
     2161 ttggccggcg gctggcggat agccggcgga gtgctcgact tctcgccgct gctgccggcg
     2221 ctgctcgact gccgcgatgc cggcgcgggt gccgcgctat tccacgccac cgtcgccgcc
     2281 ggcctcgccg agtgggcgat gcttgcggtc gaccgtgaaa aacttgcgaa aatggcggtc
     2341 ggcggcggct gcgcggcgaa cgcgatcctc ctcgacgcgc tgcgccgccg tttgacggcg
     2401 gccggcatcg aagtcctcga agcgcgcgcc gtccccgccg gcgacggcgg tctggcgctg
     2461 ggccaggcct ggatcgcccg gcaaatgcac ggagacaagg catgaacgat ctggccagcg
     2521 ccgccatcca caccgacaac ccggcgccgc gcgtgctcgc ccgttaccgg cgctggcagg
     2581 ccgcgctgaa cggcggccac cccggcctct cggtcgacat cgccggcttc cggcgctatc
     2641 gcggcgactg gatcggcgcc gtcgtcaccc cctggttcat ccacctgctg ctgctccccg
     2701 gcggcggcga actgtggcgc gagctgccgg tcgaacagac actgcgcgtc ggcttcccgg
     2761 ccggcgatct cgacctgatc agcgaacgca gcgacgacgc cgacctgccg gcgagttttt
     2821 tcgtccccat cctggcgccg gtcaacgaac tcgccgacca ggacgacgca ttgcgcaccg
     2881 cgatggatgc gctgcacctg cttttcgaac cgccggccgc cgcgccgacg cccacgaacc
     2941 ccgagccgca accggtcgac cggcgcggct tcttccgccg cctggccggg cgcggttgaa
     3001 ctcccccgaa aggaagcccc atgtgtctcg cgattcccgc cctggtcgtc gaactgcgtg
     3061 acggcgacaa tgccgtcgtc gatcttgccg gcgtgcgcaa ggaaatctcg ctgtcgctgg
     3121 tcgaaggcgt cgccgtcggc gactacgtca tcgtccatgt cggtttcgcg ctgaacaagc
     3181 tcgacccgga agaagccgag cagacgctca agctgtttgc cgaaatgggc cagttgaccg
     3241 ccgacggcga cggcctgacg ctgcactgat gaagtacatc gacgaattcc gcgacggcga
     3301 cgtggcgcag aagatcgccg ccagcatccg cgccgaggcc gatcccgccc gccactacca
     3361 tttcatggaa ttctgcggcg gccacaccca cgccatctcg cgctacggcg tcagcgacct
     3421 gctgccggcc aacgtacgca tgatccacgg ccccggctgt cccgtctgcg tgttgccgat
     3481 cggccgcgtc gaccaggcca tccgcctggc gctggatgaa ggcgtgacgc tatgcaccta
     3541 cggcgactgc ctgcgcgtgc cggcttccga cagcctgtcg ctgctgaagg cgaaagcgaa
     3601 gggcggtgac atccgcatgg tctattcgag tgccgacgcc gtgacattgg cgcaaaagaa
     3661 cccggacaaa caggtcgtct tcttcgccat cggcttcgaa acgacgacgc cgcccaccgc
     3721 tgtagcgatc aagcaggccg ccgcgctcgg cctgaagaat ttttccgtgc tctgctgcca
     3781 cgtgctgaca ccatcggcga tttccagcat cctcgaatcg ccggaagtgc gccagtgggg
     3841 taccgtgccg ctcgacggct tcgtcggccc ggcgcacgtg tcgacgatca tcggcagccg
     3901 gccctatgaa ttcttcgccg aggaataccg aaaaccggtg gtcatcgccg ggttcgagcc
     3961 gctcgacgtg atgcaggcga tccgcatgct gatccgccag gtcaacgaag gccgcgccga
     4021 agtcgaaaac gagttctcgc gcgccgtcga ccgcgacggc aacctgaagg cgcaggcgct
     4081 ggtcgccgaa gtcttcgaga tgcgcaagca cttcgaatgg cggggcttga acgtgctgcc
     4141 gtactcggcc ttgcgcattc gcggcgcgtt cgccgaattt gacgcggaga agcgctttcc
     4201 tgtgacctat gcgtcggtgg cggatcacaa ggcttgtgaa tgcggggcga ttttgcgcgg
     4261 ggtcaagcgg ccgcaggact gcaagatctt cgggacggta tgtacgccgg agaatccggt
     4321 ggggtcgtgc atggtgtcgt cagaaggtgc ctgtgcggcg cattacactt atgggcggta
     4381 taaggatgtg gcctgaggcg cgggcctttt tggtcactga aacactgggt tccgcgcctg
     4441 gcgggcgggc gtggttgttt gctgaaccac tgggttccgc cgttgaccgg cgggcggctt
     4501 tggcttccga cgcgccgttt tccgcgcctg acgcgcgggc gtactttctt ttttttggaa
     4561 aaaagaaagt agccaaagaa aaagccaccc caggttacgc ggtcggctac
//
LOCUS       DAOSDX010000099         4607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6586_length_4607_cov_4.036028, whole genome shotgun sequence.
ACCESSION   DAOSDX010000099 DAOSDX010000000
VERSION     DAOSDX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6586_length_4607_cov_4.036028"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLN02_03170"
     CDS             <1..779
                     /locus_tag="PLN02_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51351.1"
                     /translation="PKGRLATLSRDGQWTTVAAWDNSCPSNLTPAAGGLAFSDHCRFV
                     LGRVAVDGKLSEQKLPDATYIQQMSAAPDGVLWFSGDQRGRIGRIAADGAVAYLPERP
                     ASDDQAMAIKVLRNGDVVFSEFYNYNINRRTKDGDYVEHLVNVEERRGSREVRDGEVC
                     YVQFAARIAGKAEMDSKRAEEVRSGRFKADGAGTEKLVEQKCLACHDARRLLLSRRSD
                     WTPSLTRMHSYRQLRGVAPLTPEETTRLVRYFNSYYGLAR"
     gene            825..2306
                     /locus_tag="PLN02_03175"
     CDS             825..2306
                     /locus_tag="PLN02_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOI51352.1"
                     /translation="MFASALMVQGTTSDAGKSTLVAALCRIFKRRGIKVAPFKPQNMA
                     LNSAVTVDGGEIGRAQALQALACGLEPHTDFNPVLLKPTTDRRAQVIIHGQVATDLDA
                     KAYHEYKPRAMGAVLESWARLTAHYDCVLVEGAGSPAEINLRDRDIANMGFAEAVDCP
                     VIIVADIDRGGVFAHLVGTLELLSASEQARVVGFVINRFRGDIALLEPGLDWLEQRTG
                     KPVLGVLPYLHGLMLDAEDAIATAAVDGKQAAKIRVVAPAYPRTSNHNDLDPLRLHPQ
                     VDFRWIAPGEALPPADLIVLPGSKAVRADLDWLRTQGHDQAIARHLRYGGKVIGLCGG
                     YQMLGRSIADPLGLEGSPGTTPGLGFLAVDTTLECEKQLRNVSGHLRLEGQPAMIGYE
                     IHLGVTQGDGLTRAAVQLADGRADGAISADDQLLGTYCHGVFEHPEALTALLRWAGVG
                     EVDRVDFAARREADLERLADSVEAAFDWQKLSVVLPLLAVNTP"
     gene            2399..3070
                     /locus_tag="PLN02_03180"
     CDS             2399..3070
                     /locus_tag="PLN02_03180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI51353.1"
                     /translation="MSQAGPIELEFSRKYDRKHAQQYFDKHRAGPMRRLSDWREQRIA
                     RAALAMAGDPASVLDLPCGAGRFWPMLAERSERRILAADNSADMIAVAQATQAPEIVA
                     GVEAFQTSAFAIDLGDGAVDCVFSMRLLHHIAEPAHRLAMLREFHRVSCESVVLSLWV
                     DGNFKAWRRARLEARRQVNNNRFVVPRRVIEAEFAEAGFDIVGHRDFLPGYAMWRVYT
                     LRKRG"
     gene            complement(3071..3631)
                     /gene="cobU"
                     /locus_tag="PLN02_03185"
     CDS             complement(3071..3631)
                     /gene="cobU"
                     /locus_tag="PLN02_03185"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOI51354.1"
                     /translation="MPAMAKRTLILGGARSGKSRHAEQIAAASGQRVVYVATAESRDG
                     EMVRRIAHHRARRPADWGCVEEPLYLATRLRELAAPDSCLLVDCLTLWLSNLLFAGRA
                     AAQAETGEAISCPLFAGETAALVETLPQLPGQVILVSNEVGWGIVPMHPVSRLFADEQ
                     GRLNQRVAAVCERVTLVAAGLPLTLK"
     gene            3669..>4607
                     /locus_tag="PLN02_03190"
     CDS             3669..>4607
                     /locus_tag="PLN02_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51355.1"
                     /translation="MNSFTAKNFYNKIIVQNFCCETAMKISVGNPVEGEDFFDREQEQ
                     ARAWRKLAGSHLLMLAPRRIGKTSLILRLCATARQHGRYAVHCSFAKCENESDCIREL
                     FKALATEHTIEQRRKALFDKIKSVKLGALGIDWHEARPESWREAGEELGKALADGGDD
                     WLVCVDELPVFIINLLQQGDDGRRRARAFLYWLRDLRQTHYRRIKWLLAGSIGLDTLA
                     ARLGLSDTINDLEPFPLDAFSEESALRFLGELARSYAIDLDEGLRAVIVRRVGWPVPY
                     YLQLMFEHLREECVESGRTPDAAMVERVFEKLLGMGY"
BASE COUNT          817 a         1433 c         1520 g          837 t
ORIGIN      
        1 aacccaaggg ccggttggcg acgctgtcgc gcgatggcca atggaccacg gtggcggcct
       61 gggacaacag ttgcccgagc aacctgacgc cggccgccgg cggcctcgcc ttctccgacc
      121 actgccgttt cgtgttgggc cgggttgccg tcgatggcaa actcagcgaa cagaaactgc
      181 ccgacgccac ctacatccag caaatgtcgg cggcgccaga cggcgtgctg tggttttccg
      241 gcgaccaacg cggccgtatc gggcgcatcg ccgcagacgg cgcagtcgcc tatctgccgg
      301 aacggcccgc cagcgacgat caggccatgg ccatcaaggt gctgcgcaac ggcgacgtcg
      361 tgttctccga gttctacaac tacaacatca accgccggac gaaggacggc gactacgtcg
      421 aacacctggt caatgtcgag gaacgccggg gcagccgcga agtgcgcgac ggcgaagtct
      481 gctacgtcca gttcgccgcg cgcatcgccg gcaaggccga gatggacagc aaacgcgccg
      541 aggaagtgcg cagcggccgc ttcaaggccg acggcgccgg tacggaaaag ctggtcgaac
      601 agaaatgtct ggcctgccac gatgcccgcc gcctgctgct gtcgcggcgc agcgactgga
      661 cgccgagcct gacgcgcatg cattcctacc gccaactgcg cggcgtcgcg ccgctgacgc
      721 cggaggagac gacacggctg gtgcgttact tcaatagcta ttacgggttg gcgcgttgag
      781 aggagggcaa tgcgataatg cgcctcccgt tttccgaggc tgccatgttc gcttccgccc
      841 tgatggtgca aggcaccact tccgacgccg gcaaatcaac gctggtcgcc gcgctgtgcc
      901 gcattttcaa gcgccgtggc atcaaggtgg cgccgttcaa gccgcagaac atggcgctca
      961 attcggcggt caccgtcgat ggtggcgaaa ttggccgggc gcaggcgctg caggcgctgg
     1021 cctgcgggct ggaaccgcat accgacttca acccggtgct gctgaagccg accaccgacc
     1081 gcagggcgca ggtgatcatc cacggtcagg ttgccaccga cctcgatgcc aaggcctacc
     1141 atgagtacaa gccgcgcgcg atgggcgctg tgctcgaatc ctgggcgcgg ctgaccgcgc
     1201 actacgactg cgttctcgtc gaaggcgccg gctcgccggc cgagatcaac ctgcgcgacc
     1261 gcgacatcgc caacatgggc tttgccgagg ccgtcgattg cccggtgatc atcgtcgccg
     1321 acatcgaccg cggcggcgtt tttgcccacc tggtcggcac gctggaactg ttgtcggcgt
     1381 ccgagcaggc gcgcgttgtc ggtttcgtca tcaaccgttt tcgcggcgac atcgcactgc
     1441 tcgagcccgg tctcgactgg ctcgaacagc gcaccggcaa gccggtgctt ggtgttctgc
     1501 cctacctgca cgggctgatg cttgacgctg aggacgcgat cgcgacggct gcggtggacg
     1561 ggaagcaagc agcaaaaatc cgcgttgttg cgccggccta tccgcgcacc tcgaaccaca
     1621 acgatcttga tccactgcgc ctgcacccgc aagtcgactt ccgctggatc gcgccgggcg
     1681 aggcgctgcc gccggccgac ctgattgtcc tgcctggatc gaaagcggtg cgcgccgacc
     1741 tcgactggct gcgcactcag ggccacgatc aggccattgc ccgccatctg cgctacggcg
     1801 gcaaggtgat cggcttgtgt ggtggctacc agatgctcgg gcgcagcatc gccgacccgc
     1861 tcggtctcga aggatcaccg ggaacgacgc ccggtctcgg cttcctggcg gttgatacga
     1921 cgctcgaatg cgaaaagcag ttgcgcaatg tgagcggtca cttgcgtctc gaaggacagc
     1981 cggcgatgat cggctacgaa atccaccttg gcgtcactca gggcgatggc ctgacgcggg
     2041 ccgccgtgca gcttgccgac ggccgtgccg acggagccat ctcggctgat gatcagctac
     2101 tcggcaccta ctgtcacggc gtcttcgagc atcccgaggc gttgaccgcg ctgttgcgct
     2161 gggccggtgt cggcgaggtt gatcgggtcg atttcgcggc gcgccgcgaa gccgatctgg
     2221 aacgtctggc cgattcagtc gaagcggcat tcgactggca aaagctgagc gtcgtgctac
     2281 ccctgcttgc tgtgaatacg ccctagaaac atccggtagc aatctgtcga gattcgttca
     2341 tgtttggtta agggggaggc ctctagtatc cgggttcgca gatacccgga tatcctccat
     2401 gagccaagca ggaccgatcg aactcgagtt ctcccgcaag tacgaccgaa agcacgccca
     2461 gcagtatttc gacaagcatc gggctggccc gatgcgccgt ctttccgact ggcgcgagca
     2521 gcggatcgcg cgggcggcgc tggccatggc tggagacccg gccagcgtgc ttgatctgcc
     2581 ctgcggcgcc gggcgcttct ggccgatgct ggcggaacgg agcgagcgtc gcatcctcgc
     2641 cgccgacaac tcggccgaca tgattgccgt ggcccaggcc acgcaggcgc cggagatcgt
     2701 tgccggcgtc gaggccttcc agacctcggc cttcgccatc gatctgggcg acggcgctgt
     2761 tgactgcgtg ttcagcatgc gcctgctgca ccatatcgcc gaaccggcgc accggctggc
     2821 gatgctgcgc gaatttcacc gagtttcctg tgaaagcgtc gtcctttcgc tttgggtgga
     2881 cggcaatttc aaggcctggc ggcgggcccg gctggaagcg cggcggcagg tcaacaacaa
     2941 ccgcttcgtc gtgccgcgcc gcgtcattga ggcggagttc gctgaggccg gcttcgacat
     3001 cgtcggccat cgggattttc tgcccggcta cgcgatgtgg cgggtttata cgttgcgcaa
     3061 gcgcggctga ctatttcagc gtcagcggca ggccggcggc gaccagcgta acccgctcgc
     3121 agaccgccgc gacacgctgg ttgagccggc cctgttcgtc ggcaaacagg cgcgagaccg
     3181 gatgcatcgg cacgatgccc cagccgacct cgttggagac cagaatgacc tgcccgggta
     3241 gctgcggcag cgtttcgacc agcgccgccg tttctccggc gaacagcggg caggaaatgg
     3301 cttcgccggt ttccgcctgc gccgcagcgc ggccggcgaa gagcaggttg gacagccaca
     3361 gcgtcaggca atcgaccagg aggcaggagt cgggcgccgc cagttcgcgc aggcgggtag
     3421 ccagatagag cggttcctcg acgcagcccc agtcggccgg gcggcgtgcg cggtggtggg
     3481 cgatgcggcg caccatctcg ccatcgcgcg actcggcggt ggcgacgtag acgacccgct
     3541 ggccggatgc ggcggcgatt tgttcggcgt ggcggctctt gcccgaacgg gcgccgccga
     3601 ggatcaaggt tctttttgcc atggccggca ttgtggcgtc tcccttgttt ttagtgaagg
     3661 tttgagcagt gaatagcttt acagcaaaaa atttttacaa caaaataatt gtacaaaatt
     3721 tctgttgtga gacggcgatg aaaatcagtg tcggtaatcc ggtggaaggt gaggactttt
     3781 tcgaccgtga gcaggagcag gcgcgcgcct ggcgcaagct ggcggggagc cacctgctga
     3841 tgcttgcgcc acggcgcatc ggcaagactt cgctgatcct gcgcttgtgt gccactgccc
     3901 ggcagcatgg gcgctacgcc gtacattgca gttttgccaa gtgtgaaaac gagagcgact
     3961 gcatccgcga attgttcaag gcactggcga ccgagcacac catcgaacag cggcggaagg
     4021 ccttgttcga caagatcaaa agcgtcaagt tgggggcatt gggtatcgat tggcacgaag
     4081 ctcggcccga aagctggcgc gaggccggcg aggaactggg caaggcactg gcggatggcg
     4141 gcgacgactg gctggtgtgt gtcgatgaac ttcccgtctt catcatcaac ctgctgcaac
     4201 agggcgatga cggtcgtcgt cgggcgcggg cctttctcta ttggctgcgc gatctgcggc
     4261 aaacccacta ccggcggatc aaatggttgc ttgccggttc catcggtctg gacacgctcg
     4321 ccgcccggtt gggcctgagc gatacgatca acgaccttga gccttttccg cttgatgcat
     4381 tcagcgagga aagcgctctt cgctttttag gtgaacttgc caggagctac gccatcgatc
     4441 ttgacgaagg tttgcgtgca gtcatcgtcc ggcgggtagg ctggccggta ccttactacc
     4501 tgcaactgat gttcgaacac ttgcgcgaag aatgcgtgga aagcggtaga acgccggatg
     4561 cggcaatggt cgagcgggtg ttcgagaaac tgctgggcat gggttat
//
LOCUS       DAOSDX010000100         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6636_length_4586_cov_2.869124, whole genome shotgun sequence.
ACCESSION   DAOSDX010000100 DAOSDX010000000
VERSION     DAOSDX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_4586_cov_2.869124"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..295
                     /locus_tag="PLN02_03195"
     CDS             95..295
                     /locus_tag="PLN02_03195"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HOI51356.1"
                     /translation="MSILLFAQIYLIEGRTDEQKKLLIEKVSAAMAEALGAPLENTRV
                     WIQDVPKGQWGIGGKTAKDLGR"
     gene            549..1517
                     /locus_tag="PLN02_03200"
     CDS             549..1517
                     /locus_tag="PLN02_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOI51357.1"
                     /translation="MGRISAARRLPTTSVIAASFLLAACTQAPDMSGIASERARPVQR
                     YDISQAIASNGEAIVVGTQSGVTLVSHDQGKTWERQALGHVSLVDIAVCSDKTFIAID
                     HYRKLWHADASGANWQSVGLEQPRIPIAVACSPQGGWWVAGNNATIAGSADQGKTWQV
                     TDLGEDTQITALQFIDAQRVVAFGEFGLSLVSTDGGASWQKNPPLPGEFYPYAALFRN
                     AGEGWVSGIAGQILRTRDGGQTWQKQENATQLPLNRLFMHDGAPFGVGNGGVVARLEG
                     EVWRNVPYPDPLPMFLGGGASIAGQAAIVVGGPGGLLRTISTANNK"
     gene            1535..3925
                     /locus_tag="PLN02_03205"
     CDS             1535..3925
                     /locus_tag="PLN02_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOI51358.1"
                     /translation="MVSQLRHSITHRLHVGEEWIFRNPKIVLSIILAVTFLFGLALPK
                     LRIFTDFSDLLPQNHAYIKVYNRLKENFGGANMIIMSVEVEQGTIFNDDTLSLIHEAT
                     QGVDNLPGVNHNLVSSLTHRTARKVFLDDQGGFASESYYDPLQPTRTVAELERLKNDV
                     IASPTIYGLLVSPDLKAALIKAQLNEADIDYAATFAALQEVRAKLEKPGHKIYVTGNP
                     VLTGWVYTYLNQIVEILAYTLALLATLLIVYFRRFYGVALPLLGIALSSIWGLGFMAI
                     MGINLEPLSLPIPFLIAARATSHGVQLVVRYYEELALVKNGPQAARNALDALFRPGSL
                     AIIVDAVGIAVLVLGAAPFNHKLGIAAGFWGFAVIFTVHFMVPLALTILPAPKKHENG
                     NEGIRQFLGKAMAMTGGTDGGARSILILTLIGAIGATYLVGKVQLGESEPGSPLLHRD
                     HDYNVSTTAINTRFPGSEELHVIARTDEPGGIKRPEVMAAIERFQAHMLSDPTLGGTK
                     AIPGVVRVVNKLIHNDDPRWMQLPDSQDEIGGLMFTYMASSPIPGALKEFVSPDENEA
                     DMVFYYKDHQAETINRIVALAEEGIRKIEAEVPGLHIELGGGIIGVTAAGNQALHTDH
                     MIIIPAVMLLAFVIVMAYYSSLHAGWLMVLPMLFATLMTYAFMGALDIGINVNTVPVI
                     AVGVGVGIDYAVYFMDRIREEFAVLRDIKQSVINAVATTGYAVSFTAATLIAGVVMWI
                     FMSDLRFQSDAAILLSFMLIVNAIAAVLIVPSWCVVFKPKFVTAVHYDEDGVLEND"
     gene            3989..>4586
                     /locus_tag="PLN02_03210"
     CDS             3989..>4586
                     /locus_tag="PLN02_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HOI51359.1"
                     /translation="METLNNGAWQRSLLAIAVSCAFAGPAGADELPAMGEESSPWQVD
                     VTYENHTVRREHVGLAKFRNTLQAEFDKGVGNGWKVHGIMRGSWDGVYRMNSDEYGNK
                     AGGSINMQSTTPGGYLTTPWGTGPVTYSLVNGAFGLVNNAFVDAYPASNPNAGLRVLG
                     DRWHSINGGVAFAVPVRPCDVDKRGCRDFGGYGDLKRSE"
BASE COUNT          878 a         1495 c         1432 g          781 t
ORIGIN      
        1 caagagcaag aaaaagagca agaaaaagaa aggaaattat tttcttttcc gctcaacatc
       61 tttgttccta agttggtaga aaaccccatg atgattgtct atattgcttt tcgcccagat
      121 ttacctgatc gaaggtcgca ccgacgaaca gaagaagctg ctgatcgaga aggtcagcgc
      181 cgcgatggcc gaagccctcg gcgcgccgct ggaaaacacc cgggtgtgga tccaggacgt
      241 gccgaaaggg caatggggca tcggcggcaa gaccgccaag gacctcgggc gctagccgaa
      301 cgatgcaacg ggctgcccgg cgaagcgccg ggcagcgccg ggtcggcctg gctcaggcaa
      361 tacctgaaag gtatctattg aggcagtaaa tggtattgga cggtattggc gccattcggc
      421 catagtaggg tcagtaaaaa aatatcgaga ttttgtgggg ccgcagccca gatgctgccg
      481 gcaaccgcag gagacaaggc aagtcagggt tggtcggtaa gcgaccgacc cgcaaaggag
      541 gaagttttat ggggcggatt tctgccgcaa gacgattgcc gaccacctcg gtgatcgcag
      601 cgagtttcct gctggccgcg tgcacgcagg cgccggacat gtccggcatt gccagcgagc
      661 gggcgcggcc ggtgcagcgt tacgacatca gccaggccat cgccagcaac ggcgaggcca
      721 tcgtcgtcgg cacccagagt ggcgtcacac tggtttcgca cgaccagggc aagacctggg
      781 aacggcaggc gctcggccac gtgtcgctgg tcgatatcgc cgtctgcagc gacaagacct
      841 tcatcgccat cgaccactat cgcaagctct ggcatgccga cgcgagcggc gccaactggc
      901 agtcggtcgg gctcgaacag ccgcgcattc cgattgccgt cgcctgcagc ccgcagggcg
      961 gctggtgggt ggccggcaac aacgcgacga tcgccggcag cgccgatcag ggcaagacct
     1021 ggcaggtcac cgacctcggc gaggataccc agatcaccgc cctgcagttc atcgacgcgc
     1081 agcgcgtcgt tgccttcggc gagttcggcc tgtcgctcgt gtcgaccgac ggtggcgcca
     1141 gctggcagaa gaatccgcca ctgcccggcg agttctaccc ctacgccgcg ctctttcgca
     1201 atgccggcga aggctgggtg agcggcatcg ccggccagat cctgcgcacc cgcgacggcg
     1261 ggcagacctg gcagaagcag gagaacgcca cgcaactgcc gctcaaccgc ctgttcatgc
     1321 atgacggcgc gccgttcggc gtcggcaacg gcggtgtcgt ggcgcggctg gaaggggagg
     1381 tctggcgcaa cgtgccgtat cccgatccgc tgccgatgtt cctcggcggt ggcgcttcca
     1441 tcgccggtca ggcggcgatc gtcgtcggcg gcccgggcgg cctgctgcgc acgatcagca
     1501 cggcgaacaa caagtagaac aggaagcaag catcatggtc agtcaactcc gtcattccat
     1561 cacgcatcgc ctgcacgtcg gcgaagagtg gattttccgc aacccgaaga tcgtcctctc
     1621 gatcatcctg gcagtcactt tcctgttcgg cctggcgctg cccaagctgc gcatcttcac
     1681 cgacttttcc gacctgctgc cgcagaacca cgcctacatc aaggtctata accggctgaa
     1741 ggagaatttc ggcggtgcca acatgatcat catgtcggtc gaagtcgagc agggaacgat
     1801 cttcaacgac gacaccctga gcctgatcca cgaggcgacg cagggcgtcg acaacctgcc
     1861 cggggtcaac cacaacctgg tttccagcct gacccaccgc acggcgcgca aggtcttcct
     1921 cgacgaccag ggcggtttcg cgtccgagtc ctactacgat ccactgcagc cgacgcgcac
     1981 cgtcgccgag ctggaacggc tgaagaacga cgtcatcgcc agcccgacca tctacggcct
     2041 gctcgtctcg cccgacctca aggcggcgct gatcaaggcc cagctgaacg aggccgacat
     2101 cgactacgcc gccaccttcg ccgcgctgca ggaagtgcgc gccaagctgg agaagcccgg
     2161 tcacaagatc tacgtcaccg gcaacccggt gctcaccggc tgggtttaca cctacctcaa
     2221 ccagatcgtc gaaatcctcg cctacacgct ggccctgctg gcgacgctgc tgatcgtcta
     2281 tttccgccgc ttctacggcg tcgcgctgcc cttgctaggg attgcgctgt cgtcgatctg
     2341 gggcctcggt ttcatggcga tcatgggcat caacctcgag ccgctgtcgc tgccgattcc
     2401 cttcctgatt gccgcccgcg ccacttcgca cggcgtgcaa ctggtggtgc gctattacga
     2461 ggaactggcg ttggtcaaga acggcccgca ggcggcgcgc aacgcgctcg atgcgctgtt
     2521 ccggcccggt tcgctggcga tcatcgtcga cgccgtgggg attgccgtgc tcgttctggg
     2581 cgccgcgccg ttcaaccaca aactcggtat tgccgccggc ttctggggct tcgccgtgat
     2641 cttcacggtg cacttcatgg tgccgctggc gctgaccatc ctgccggcgc cgaagaagca
     2701 cgagaacggc aatgaaggca tccgccagtt cctcggcaag gcgatggcaa tgaccggcgg
     2761 caccgacggc ggtgcccgct cgatcctgat cctgacgctg atcggcgcga tcggtgcgac
     2821 ctacctggtg ggcaaggtgc agctcggcga atccgagccg ggttcgccgc tgctccaccg
     2881 cgaccacgac tacaacgtgt cgacgacggc gatcaacacc cgcttcccgg gttccgagga
     2941 actgcacgtc atcgcccgca ccgacgaacc cggcggcatc aagcggccgg aagtgatggc
     3001 ggcgatcgag cgcttccagg cgcacatgct gtccgacccg acgctgggcg gcaccaaggc
     3061 gatccccggc gtcgtgcgcg tggtcaacaa gctgatccac aacgacgatc cgcgctggat
     3121 gcaactgccg gacagccagg acgagatcgg cggcctgatg ttcacctaca tggcgtcgag
     3181 cccgatcccg ggggcgctca aggagttcgt cagcccggac gagaacgagg cggacatggt
     3241 gttctattac aaggaccacc aggcggaaac gatcaaccgc atcgtcgccc tggccgaaga
     3301 aggcatccgc aagatcgagg ccgaagtgcc gggcctgcac atcgaactgg gcggcggcat
     3361 catcggcgtc acggcggccg gcaaccaggc gctgcacacc gaccacatga tcatcatccc
     3421 ggccgtcatg ctgctcgcct tcgtcatcgt catggcctac tacagctcgc tgcacgccgg
     3481 ctggctgatg gtcctgccga tgctgttcgc gacgctgatg acctatgcct tcatgggcgc
     3541 gctcgacatc ggcatcaacg tcaacacggt gccggtgatc gccgtcggcg tcggcgtcgg
     3601 catcgactac gcggtctatt tcatggaccg catccgcgag gagttcgccg tcctgcgcga
     3661 catcaagcag tcggtgatca acgccgtcgc caccaccggc tacgccgtca gcttcaccgc
     3721 ggcgacgctg atcgccggcg tcgtgatgtg gatcttcatg tccgacctgc gcttccagtc
     3781 ggacgccgcg atcctgctgt ccttcatgct catcgtcaat gcgatcgccg ccgtgctgat
     3841 cgtgccgtcc tggtgcgtcg tcttcaagcc gaagttcgtc accgcggtgc attacgacga
     3901 ggacggcgtc ctggaaaacg actaacaaga cccggcaaac cggggaacag caaaaccttt
     3961 gttcacaacc agatggggga tcaaaaacat ggagacactc aataacggag catggcagcg
     4021 cagcctgctc gcaatcgcgg tgagctgcgc cttcgccggt ccggctgggg ccgacgagtt
     4081 gccggcgatg ggcgaggaaa gctcgccctg gcaggtggac gtgacttatg aaaaccatac
     4141 cgtgcgtcgc gaacacgtcg gcctggccaa gttccgcaac accctgcagg ccgagttcga
     4201 caagggcgtc ggcaacggct ggaaggtgca cggcatcatg cgcggcagct gggacggcgt
     4261 ctatcgcatg aatagcgacg agtacggcaa caaggcgggc ggttcgatca acatgcagtc
     4321 gacgacgccc ggtggttacc tgacgacgcc gtggggcacc gggccggtga cctattcgct
     4381 ggtcaatggc gccttcggcc tggtgaacaa cgcctttgtc gatgcctacc cggcgagcaa
     4441 cccgaacgcc ggtctgcgcg ttctcggcga ccgctggcac agcatcaacg gcggtgtcgc
     4501 ctttgccgtg ccggtccgtc cctgcgacgt ggacaagcgc ggctgccggg atttcggcgg
     4561 ctacggcgac ctcaagcgtt ccgagc
//
LOCUS       DAOSDX010000101         4585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6639_length_4585_cov_3.815673, whole genome shotgun sequence.
ACCESSION   DAOSDX010000101 DAOSDX010000000
VERSION     DAOSDX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6639_length_4585_cov_3.815673"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLN02_03215"
     CDS             <1..965
                     /locus_tag="PLN02_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOI51360.1"
                     /translation="GALALFGVASLGCMLASSIGQLWFWRAIQGITAGAGIVISRAIV
                     RDLYDGADAQRLMAQITMMFALAPAIAPVIGGWLQSAFGWRSVFAFLSLSTIVLLIAC
                     LHLLPETLPPEKRQSLRPAYLASTYRKVFSSPRFLFACAALALNFGGFFIYVLSSPVF
                     LMRHLGLPETAFLWLFGPAMLGLMSGSWCSGRLAGRLAPGRTIALGYLVMGCAASLNL
                     ALNLSLPPGLPWSVAPLFVYTLGMSLAMPNLTLFALDPFPAQRGLAASCQTFFQSGFN
                     GVAAALIAPALWSSTLHLALGMAGLLAIGGLAALAHRRLRLLPG"
     gene            complement(945..1337)
                     /locus_tag="PLN02_03220"
     CDS             complement(945..1337)
                     /locus_tag="PLN02_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI51361.1"
                     /translation="MQHLDPHAAHLFLQQTPEAVLVDCRTEIEHMYVGHPIGAEHVAW
                     QEGPDWEIDPDFAAKVKRLLRGELARPVLLICRSGVRSLAAGEALEAAGFTQVINVVE
                     GFEGPLDEDFHRGTLGGWRYRGLPWQQS"
     gene            1428..1925
                     /locus_tag="PLN02_03225"
     CDS             1428..1925
                     /locus_tag="PLN02_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51362.1"
                     /translation="MKLPGWIATVFSALAATLLPACDAVNLPEIKPGITTLAEVRSRM
                     GEPGFIHRNDDGTATWEYSRQPAGIHCYMISFDCRDIVSELEQVLTPENLARVAPGMT
                     QQELRRRLGQPARREVFSNLGEEVWEWHIAGDIPTEETYFSAHFDLGDGTLRKAGQRI
                     EPKGR"
     gene            complement(1922..2524)
                     /gene="mobA"
                     /locus_tag="PLN02_03230"
     CDS             complement(1922..2524)
                     /gene="mobA"
                     /locus_tag="PLN02_03230"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HOI51363.1"
                     /translation="MHNKCSPERSRITGVVLAGGLGRRMGGVDKGLQELDGRPLVAHV
                     IARLAPQVGSLLINANRNTERYAAFGYPVVADAIDGFAGPLAGLHAALAAATTPYVAT
                     APCDSPFLPDDLVPRLFAALTANAAQIAVARTGEQTHPVFCLCRRELLPSLAAWLQAG
                     ERRFGGWTATLAAVEVAFDDQSAAFENINTREELARYGGA"
     gene            complement(2541..3428)
                     /gene="fdhE"
                     /locus_tag="PLN02_03235"
     CDS             complement(2541..3428)
                     /gene="fdhE"
                     /locus_tag="PLN02_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOI51364.1"
                     /translation="MQFPPQTLPSTDDYPSLLRPGTSVFARRASRLRTLAGASPAADW
                     LNRLALLCDAQQTAFDALGDLGLRLPAGFDWQQAAAAGRQAEIEAAWPAIYPQLAGAM
                     AAAGVAIADLEAAKASLPAVRALLEGEAEAALRAPTEAVLAAALQVVWTRFAQGLDVP
                     AGKVLLAERGHCPCCGSMPLGSIVFAGQGRGGARYQECSLCATRWNAVRARCTLCDDP
                     GVVAYLGIEDAFPAVRAEACEHCKGYSKLYFQDKDLAVDPLADDLASLALDVLVGEQG
                     YARGGPNPFLLLGGNAEPD"
     gene            complement(3442..4323)
                     /gene="fdhD"
                     /locus_tag="PLN02_03240"
     CDS             complement(3442..4323)
                     /gene="fdhD"
                     /locus_tag="PLN02_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOI51365.1"
                     /translation="MSLRNLSLADTGSEEEMPAVVSTGVHRLRGDAWSATSDDLIEEV
                     PVALVYNGISHAVMLASPADLEDFALGFSLSEGILRRPGELYDIEIGTACDGITLDLS
                     IAHERFAGLKERRRQLAGRTGCGLCGVDSLAALPAMPQSVPPGAAAISLAAIREALRQ
                     LPHCQTLRQRCGSAHAAAWADEQGNIVCVREDVGRHNALDKLIGALARGKVASAGQGF
                     VVVTSRASYEMVQKTASAGLSALVAVSAPTALAVRQARAAGLLLAGFARAEGLVVYAG
                     AERLCGDGVGPAPAILM"
BASE COUNT          741 a         1620 c         1497 g          727 t
ORIGIN      
        1 tcggcgcgct cgcgctgttc ggcgtcgcct cgctcggctg catgctcgcc agcagcatcg
       61 ggcaactgtg gttctggcgc gccatccagg gtatcaccgc cggcgccggc atcgtcatca
      121 gccgtgccat cgtccgcgat ctctacgatg gcgccgacgc gcaacgcctg atggcgcaaa
      181 tcaccatgat gttcgcactg gcgccggcca ttgcgccggt aatcggcggt tggctgcaaa
      241 gcgctttcgg ctggcgctcg gtcttcgctt tcctgagcct gtcgaccatc gtcttgttga
      301 tcgcctgtct ccacctgctg ccggaaaccc tgccgccgga aaagcgccag tcgctacgcc
      361 cggcctatct cgccagcacc tatcgcaagg tcttcagctc gccccgcttc cttttcgcct
      421 gcgccgcgct ggcgctcaat ttcggcggct tcttcattta cgtgctgtcg tcgccggtat
      481 tcctgatgcg tcacctcggt ctgccggaaa ccgccttcct ctggctgttc ggcccggcga
      541 tgctgggact gatgagcggt tcatggtgtt ccggacggct cgccggccgc cttgccccgg
      601 gccgcacgat cgccctcggt tatctggtga tgggctgcgc cgccagcctc aatctggcac
      661 tcaacctgag tttgccgccc ggcctgccgt ggagcgtcgc gccgcttttc gtctacaccc
      721 tgggcatgtc gctggcgatg cccaacctga cgctgttcgc gctcgacccc ttccccgccc
      781 aacgcggctt ggcggcgtcc tgccagacct tcttccagtc cggtttcaac ggcgtcgcag
      841 cggccttgat tgcaccggcc ctgtggagtt caaccctgca tctggcgctg ggcatggccg
      901 gcctgctcgc catcggcggc ctggccgccc tcgcccaccg gcggctaaga ttgctgccag
      961 ggtaggccgc gatagcgcca gccacccagc gtgccgcgat gaaaatcctc gtcgagcggc
     1021 ccctcgaagc cttccacaac gttgatcacc tgcgtgaaac ccgccgcctc caacgcctcg
     1081 ccggcggcca gcgagcgcac cccgctgcgg cagatgagca gcaccggccg ggccagctcg
     1141 cccctgagca ggcgtttcac cttggccgcg aaatccgggt cgatttccca gtccgggcct
     1201 tcctgccagg cgacgtgttc ggcgccgatc gggtgaccga cgtacatgtg ctcgatctcg
     1261 gtccggcagt cgaccaggac cgcctccggc gtctgttgca ggaaaaggtg ggcggcgtgg
     1321 gggtcgagat gctgcattgg cggtcggaaa atggatcagg agccctatta tgaaagcccg
     1381 acggcaattt gcgtaaactg agcaacatca cccaagagga aatttcgatg aaactgcccg
     1441 gctggattgc caccgtcttc tccgcccttg ccgccaccct cctgccggcc tgcgatgcgg
     1501 tcaatctgcc cgagatcaag ccgggcatca ccactctggc cgaagtgcgc agccgcatgg
     1561 gcgagcccgg cttcatccac cgcaacgacg acggtacggc gacctgggaa tacagccgac
     1621 agccggccgg catccactgc tacatgatca gcttcgactg ccgcgacatc gtcagcgaac
     1681 tggaacaggt cctgactccg gaaaacctgg cacgcgtcgc gccgggcatg acgcagcagg
     1741 agttgcgtcg ccgactcggc cagccggcgc gccgcgaggt attcagcaat ctcggcgaag
     1801 aggtctggga atggcacatt gccggcgaca tcccgaccga ggagacctat ttctcggcgc
     1861 acttcgacct cggcgacggc acgctgcgca aggccggcca gcgtatcgaa ccgaaagggc
     1921 gctaggcgcc gccgtaacgc gccagctctt cgcgcgtgtt gatgttctcg aaggccgccg
     1981 actggtcgtc gaaagcgacc tcgaccgcgg ccagcgtcgc cgtccagccg ccgaagcggc
     2041 gctcgccggc ctgcagccag gccgccagcg acggcagcaa ctcgcgtcgg cacaggcaga
     2101 ataccgggtg cgtctgctcg ccggtacggg cgacggcaat ctgcgcggca tttgcggtca
     2161 aggccgcaaa cagccgggga accagatcgt ccggcagaaa gggcgaatcg cacggagcgg
     2221 tcgccacgta aggcgtcgtt gccgccgcca gcgccgcgtg cagcccggcc agcggcccgg
     2281 cgaaaccgtc gatggcatcg gccaccaccg gatagccgaa ggcggcgtaa cgctcggtat
     2341 tgcggttggc gttgatcagc aggctgccca cctgcggcgc cagccgggca atcacgtgcg
     2401 ccaccagcgg ccgcccgtcg agttcctgca ggcccttgtc gacgccgccc atgcgccggc
     2461 cgagaccgcc ggccaggacg acgccggtga tccgggagcg ctcaggtgag catttgttgt
     2521 gcattcaccc agccttcgtt tcagtccggt tcagcattcc cgccgagcag caggaaggga
     2581 ttcggcccgc ctctggcata gccctgttcg ccgaccagga catcgagcgc gaggctcgcc
     2641 agatcgtcgg cgagcggatc gacggcgagg tccttgtcct ggaaatagag cttgctgtag
     2701 cccttgcagt gttcgcacgc ctcggcgcgg accgccggaa aggcgtcctc gatgccgagg
     2761 taggcgacca cgcccggatc gtcgcacaag gtgcaacggg cgcgcaccgc gttccagcgc
     2821 gtcgcgcaca ggctgcattc ctgatagcgg gcgccgcccc ggccctgccc ggcaaagacg
     2881 atgctgccga gcggcatgct gccgcagcac gggcaatgcc cccgttcggc cagcaacacc
     2941 ttgccggccg gcacgtccag cccctgggca aagcgcgtcc acaccacctg cagcgcggcg
     3001 gcgagcaccg cttcggtcgg cgcccgcaag gcggcctcgg cctcgccctc gagcaaggcg
     3061 cgcactgccg gcaggctggc cttggccgct tccaggtcgg cgatggcaac gcccgcggcg
     3121 gccatggcgc cggccagttg cgggtagatc gccggccacg ccgcttcgat ttccgcctgg
     3181 cgaccggccg ccgcggcctg ctgccagtca aaaccggccg gcaagcgcag gccgaggtcg
     3241 cccagcgcgt cgaacgccgt ctgctgcgcg tcgcacagca gtgccaggcg attcagccag
     3301 tcggctgccg gcgaggcgcc ggccagcgtg cgcaggcggc tggcccggcg ggcgaagacg
     3361 ctggttcccg gacgcagcag gctcgggtag tcgtcggtgg aaggcagggt ttgcggggga
     3421 aattgcatgt gttttctcgg gttacatcag gatggccggc gccgggccga ccccgtcgcc
     3481 gcacaggcgt tcggcgccag cataaacgac caagccttcg gcgcgcgcaa aacccgccag
     3541 cagcaggccg gcggcgcggg cctggcggac cgccagcgcg gtcggcgcgg aaaccgcgac
     3601 cagcgccgac aggccggcgc tggccgtttt ctggaccatt tcataggagg cccggctggt
     3661 gacaacgacg aagccctgtc cggcgctcgc caccttgccg cgcgccaagg cgccgatcag
     3721 cttgtcgagc gcattgtgcc gaccgacatc ctcgcgcacg cagacgatgt tgccctgctc
     3781 atcggcccag gccgccgcat gcgccgaacc acagcgctgg cgcagggtct ggcagtgcgg
     3841 caactggcgc agcgcttcgc ggatggcggc gagcgagatg gcggccgcgc ccggcgggac
     3901 ggattgcggc atggccggca gggcggccag gctatcgaca ccgcacaagc cgcagccggt
     3961 acgaccggcg agctggcgcc gccgctcctt gaggccggcg aaacgttcgt gggcgatgct
     4021 cagatcgagc gtgatgccat cgcaggcggt accgatctcg atgtcgtaga gctcgcccgg
     4081 gcgcctgagg atgccctccg agaggctgaa gccgagcgcg aaatcctcca ggtcagccgg
     4141 cgaggcgagc atcacggcat gcgagatgcc gttatagacg agcgcgaccg gaacttcttc
     4201 aatcaggtcg tcgctcgttg ccgaccaggc atcgccgcgc agacgatgca cgccggtgct
     4261 gacaaccgcc ggcatttcct cttccgagcc ggtgtcggca aggctcaaat tccgcagact
     4321 catggcgcta tccgttttgc caaaaaagcg gggatgcagg aaaacctgca gagccccgat
     4381 aacgcaaggt aaacaatcgc tgcaaaggcg cggcttactt gccggtgacc tggcggtacc
     4441 agccgcgatg atgctgcttg gcccaggcgc gggtgaccgt gccgtaccac atggcgcgga
     4501 tcgtaccctt gacccagatc gcggcataga cgtgcaccat gatcaggccg atcatcaccg
     4561 ccccggcagc ggcgtgaacc accgc
//
LOCUS       DAOSDX010000102         4551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6704_length_4551_cov_3.495329, whole genome shotgun sequence.
ACCESSION   DAOSDX010000102 DAOSDX010000000
VERSION     DAOSDX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6704_length_4551_cov_3.495329"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLN02_03245"
     CDS             complement(<1..415)
                     /locus_tag="PLN02_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51366.1"
                     /translation="MIDLQRRRTARPDAANALERSIHSPFIEGDVIGRVRLVPDPEAI
                     AGQVRAEIIERSLQLTWQLTLIAGAMVVTLLFFVIQPISAISTELHRMDPTAGDRLQI
                     PAGHAKTEIGRLVRDVNALAGRLVHALDEEHALRLQ"
     gene            complement(428..703)
                     /locus_tag="PLN02_03250"
     CDS             complement(428..703)
                     /locus_tag="PLN02_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51367.1"
                     /translation="MRAALTRDGILFRSILVTLLAFALVGFATLAYTVQTARSAAEEQ
                     SRVRLEQLLDTVQSTLRVACFVKDQELAREVASGLLSNTEVLRVTIH"
     gene            complement(681..1133)
                     /locus_tag="PLN02_03255"
     CDS             complement(681..1133)
                     /locus_tag="PLN02_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51368.1"
                     /translation="MPTRLFFLLLLLANCPTVDAEPVVVVAAQQATERLSREEVANIF
                     LGRYRALASGLPAQPIDQPPASALRSAFYQQLVNKDTASINAYWSRLHFSGKATPPLQ
                     TTEENEVIRQLLSIPGGIAYVERKQVDQRMRIVFEFMPRVTHESRVNP"
     gene            complement(1140..2345)
                     /locus_tag="PLN02_03260"
     CDS             complement(1140..2345)
                     /locus_tag="PLN02_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51369.1"
                     /translation="MPTRFRIICLLAVSMAIARPAYADETAPAPPWPAIEFKGFGTLG
                     VARSDSKDSQFVRDLSQPDGLGKHWSGKTDTMFGLQANLTLSPRTGAVMQGLVRYRYD
                     GSWRPELNLAFLRHDLAPDFTLRAGRMGTEFYLQADARQVGYASTMLRPPADYFGPIV
                     VSYFDGVDASLTGDTGYGLLRGKLFAGWAAEKTPVVKPHTWDLRGSRMAGAYLDYLNG
                     PWQMRVSRSQIRFQHELPLDAVAGFPILTLAPELGIEGHHATYDSLGLIYDEGRLQVQ
                     GQVSSIHYDTSAYEDTRAGYVMLAYRVGPVTPYLSYSRSRSRAASLSTPTVPALQTAI
                     RQFMAQTHSDQATYTLGARWDFQRNLALKAQLDMIRADRSSRFPQRYTQNDWDGSLNV
                     FSLALDFVF"
     gene            2581..3132
                     /locus_tag="PLN02_03265"
     CDS             2581..3132
                     /locus_tag="PLN02_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51370.1"
                     /translation="MDRYQSLFFNANFCEAASLAGCMAYRGELVLQEGEIADAQGRRK
                     PPQQVMKQAVLMTVGDELKLLIGSLDELQFMPALLDKYGADIKAGSQVVLYTVNIDQP
                     FVSTINGAQFVFIPMVQGMVWTELCETLGLEKSDFKGMGAADKVVRVYEELLVTPFKY
                     PEMALDEAMGKTNANKRENHGAI"
     gene            3132..3512
                     /locus_tag="PLN02_03270"
     CDS             3132..3512
                     /locus_tag="PLN02_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOI51371.1"
                     /translation="MPWQSEMVLGVAEMDATHRAFVDLADALLAASDAEFPALFAQLR
                     EDTRRHFENEDKLMVACRFPATGEHRAEHLRVLGELAFFGRGVATGRLRAAREYARSL
                     PGWFMTHLQTMDAALAARLNSAAA"
     gene            complement(3498..4298)
                     /locus_tag="PLN02_03275"
     CDS             complement(3498..4298)
                     /locus_tag="PLN02_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOI51372.1"
                     /translation="MSFLAALLQQTFLQHAVAASLLAALACGVVGVLVVLRRIAFLAG
                     GIAHGALAGMGAAYLLGWPPLPGAIAAALAMAVLLWRLTTRQRHDEEVLIAAMWSVGM
                     AAGLIMIARSAGENPDLMSYLFGNILMVGRDQLLWMAGLDLLTLGIVAAFYPAIATTV
                     FDEEYARLRGLPVEAIRLLLLLLVALTVVLLIQVVGLILVIALLTLPAAAALSSRSML
                     AMMWRAALIAGAACLGGLWLAYAQDWPAGASMVLLTAAAYLVASLRRR"
     gene            complement(4295..>4551)
                     /locus_tag="PLN02_03280"
     CDS             complement(4295..>4551)
                     /locus_tag="PLN02_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51373.1"
                     /translation="PTASIDPAGQEALYTLLGELNLQMPLLVVSHDLSLISRQARRVW
                     CLNRRLHVHPALELTAETLASLYGFPVRAVDHRSDHAETA"
BASE COUNT          783 a         1363 c         1665 g          740 t
ORIGIN      
        1 gctgcaggcg cagcgcgtgc tcctcgtcca gcgcatgcac caggcgaccg gccagcgcgt
       61 tgacgtcgcg gaccaggcgg ccgatttcgg tcttggcgtg accggccgga atctgcaggc
      121 ggtcgccggc ggtcgggtcc atccggtgca gttcggtcga gatggcgctg attggctgga
      181 tgacgaagaa cagcagggtg accaccatgg cgccggcgat caaggtcagt tgccaggtca
      241 gctggagcga acgttcgatg atttcggcgc gtacctgacc ggcgatcgcc tccgggtcgg
      301 gaacgaggcg gacgcgaccg atcacgtcgc cttcgatgaa cggcgaatgg atgctgcgtt
      361 caagggcgtt ggccgcatct ggacgagctg ttcggcgccg ttgcaggtcg atcaactggt
      421 tgtcgcccta gtggatggtg acgcgcagga cttcggtatt gctcagcagg ccgctggcca
      481 cctcgcgggc gagttcctgg tccttgacga agcaggcgac gcgcaaggtg ctctgcacgg
      541 tgtcgagcaa ctgttcgagc ctgacccgcg actgttcctc ggccgcgctg cgcgccgtct
      601 gcacggtata ggcgagggtt gcgaaaccga ccagcgcgaa ggcgaggagg gtgaccagga
      661 tcgatcggaa caggatgccg tcacgggtta acgcggctct catgagtcac cctcggcatg
      721 aattcgaaaa cgatgcgcat gcgttggtcg acctgcttgc gttcgacgta ggcgatgccg
      781 cccggaatgc tgagcaactg tcggatcacc tcgttctcct cggtggtctg cagtggcggt
      841 gttgccttgc cggagaagtg caggcggctc cagtaggcgt tgatgctggc ggtgtccttg
      901 ttcaccagtt gctgatagaa ggcgctgcgc aacgcgctgg ccgggggctg gtcgatcggc
      961 tgggccggca ggccggaggc cagggcgcgg taacggccga ggaagatgtt ggcgacctct
     1021 tcccggctca gccgctcggt ggcctgctgc gcggccacga ccaccaccgg ctcggcgtcc
     1081 accgtcgggc agttggcgag gagcagcagg aggaagaaga ggcgggtcgg catgccgggt
     1141 cagaagacga aatcgagcgc cagactgaag acgttcaggc tgccgtccca gtcgttttgc
     1201 gtatagcgtt gcgggaagcg ggagctgcgg tcggcgcgga tcatgtccag ttgcgccttg
     1261 agcgccaggt tgcgctggaa gtcccagcgg gcgcccaggg tgtaggttgc ctggtcgctg
     1321 tgggtctgcg ccatgaactg gcggatcgcc gtttgcaagg ccgggacggt cggtgtgctc
     1381 aggctggcgg cgcgcgagcg gctgcgcgag tagctgaggt agggggtcac cgggccgacc
     1441 cggtaggcca gcatcacgta gccggcgcgg gtgtcctcgt aggcggatgt gtcgtagtgg
     1501 atgctgctga cctggccctg aacctgcagt cggccttcgt cgtagatcag gccgaggctg
     1561 tcgtaagtgg cgtggtgtcc ctcgatgccg agttccggtg ccagcgtcag gatcggaaag
     1621 ccggccacgg cgtcgagcgg tagctcgtgc tggaagcgga tctgcgagcg gctgacgcgc
     1681 atttgccagg ggccgttgag gtagtcgagg taggcgccgg ccatgcgcga accgcgcaga
     1741 tcccaggtgt gcggcttgac caccggcgtc ttttccgccg cccagccggc gaacagcttg
     1801 ccgcgcagca ggccgtaacc ggtatcgccg gtcaggctgg cgtcgacgcc gtcgaaatag
     1861 gaaacgacga tcggcccgaa atagtcggcg ggcgggcgca gcatggtgct ggcgtagccg
     1921 acctggcggg cgtcggcctg caggtagaac tcggtgccca tgcggccggc gcgcagcgtg
     1981 aaatcgggcg ccagatcgtg gcgcaggaag gccagattca gctcggggcg ccaactgccg
     2041 tcgtagcgat agcgcaccag gccctgcatg acggcgccgg tgcgcgggct gagcgtcagg
     2101 ttcgcctgca gaccgaacat ggtgtcggtc ttgccgctcc agtgcttgcc cagtccgtcg
     2161 ggttgcgaca ggtcgcggac gaactggctg tccttgctgt cgctgcgggc gacgccgagc
     2221 gtcccgaaac ccttgaattc gatcgctggc cagggcgggg caggcgctgt ttcgtcggcg
     2281 taagccggcc tggcgatggc catggagacg gcaagcaggc agatgatccg gaagcgggtg
     2341 ggcatggtcg gccaatgcgc tggagggcgg ttagtctttg ttcgggatta tgcactttgt
     2401 ctagcggctt ggggttttgc gagcggggcc aaaccgacaa gcggtatgtc ggaaagccga
     2461 cagctacccc ggtgcggcat ccggattccc ttgtgcgaca aggaaggccg atccggcaag
     2521 gaaattgctg taaggccagg accaagacga atcaacttca gggaaggaag aaaaatgctg
     2581 atggaccgtt accaatcctt gttcttcaac gccaatttct gcgaagcggc gagcctggcc
     2641 ggttgcatgg cctatcgcgg cgaactggtg ctgcaggaag gcgaaatcgc cgatgcccag
     2701 gggcgccgca agccgccgca acaagtcatg aagcaggccg tgctgatgac cgttggtgat
     2761 gaattgaagc tgctgatcgg ttcgctcgac gaactgcagt tcatgccggc gctgctcgac
     2821 aagtacggcg ccgacatcaa ggccggtagc caggtcgtgt tgtatacggt caatatcgac
     2881 cagcccttcg tgtcgacgat caacggcgcg cagttcgtct tcatcccgat ggtgcagggc
     2941 atggtgtgga ccgaactgtg cgagacgctg ggcctggaaa agagcgactt caagggcatg
     3001 ggcgccgccg acaaggtggt ccgtgtgtac gaggaactgc tggtcacccc gttcaagtac
     3061 ccggaaatgg ccctcgacga ggcgatgggc aagaccaacg ccaacaagcg cgaaaaccac
     3121 ggcgccatct gatgccctgg cagagcgaaa tggttctcgg ggtggcggaa atggacgcca
     3181 cccaccgcgc cttcgtcgat ctcgcggacg ccctgctggc cgcttcggac gccgagtttc
     3241 cggcgctttt cgcgcagttg cgcgaggaca ccaggcggca cttcgagaac gaggacaagc
     3301 tgatggtggc ctgccgcttc ccggcgaccg gcgagcatcg tgccgagcat ctgcgggtgc
     3361 tcggcgaact cgcctttttc ggccgtggtg tcgccaccgg ccgcttgcgc gcggcgcgcg
     3421 aatacgcccg cagcctgccg ggctggttca tgacgcatct gcagaccatg gatgcggcac
     3481 ttgccgcgcg cctgaattca gcggcggcgt aggctggcga cgagataggc ggcggcggtg
     3541 agtagcacca tgctggcgcc ggccggccag tcctgcgcat aagccagcca caggccgccc
     3601 aggcaggcgg cgccggcgat cagcgcggcc cgccacatca tcgccagcat cgagcgggac
     3661 gacagcgcgg cggcagccgg cagggtgagc agggcgatga ccaggatcag gccgaccacc
     3721 tggatcagca ggacgacggt cagcgccacc agcaacagca gcagcaggcg gatcgcctcg
     3781 accggcaggc cgcgcaggcg ggcgtattcc tcgtcgaaga cggtggtggc aatcgccggg
     3841 tagaaggcgg cgacgatgcc cagcgtcagc aggtcgaggc cggccatcca cagcagctgg
     3901 tcgcggccga ccatcaggat gttgccgaac aggtagctca tcaggtccgg gttttcgccg
     3961 gcgctgcggg cgatcatgat cagcccggcg gccatgccga ccgaccacat ggcggcgatc
     4021 agcacctcct cgtcgtggcg ctggcgcgtg gtcagtcgcc agagcaggac cgccatcgcc
     4081 agcgcggcgg cgatggcgcc gggcagcggc ggccagccga gcaggtaggc ggcgcccatg
     4141 ccggccagcg cgccgtgggc gatgccgcca gcgaggaagg cgatgcgccg cagcacgacc
     4201 aggacgccga cgacgccgca ggccagcgcg gcaagcagcg aagcggcgac ggcgtgctgg
     4261 aggaaggtct gttgcagcag ggcggcaagg aaactcacgc ggtctccgcg tggtcgctgc
     4321 ggtgatcgac ggcgcgcacc gggaaaccgt agaggctggc cagggtttcc gccgtcagtt
     4381 cgagcgcggg atggacatgc aggcggcgat tcaggcacca gacccggcgc gcctggcggc
     4441 tgatcaggct gaggtcgtgc gagacgacga gtagcggcat ctgcagattg agttcgccga
     4501 gcagcgtata gagcgcttcc tggccggccg ggtcgatgct ggccgtcggt t
//
LOCUS       DAOSDX010000103         4533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6731_length_4533_cov_3.229567, whole genome shotgun sequence.
ACCESSION   DAOSDX010000103 DAOSDX010000000
VERSION     DAOSDX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6731_length_4533_cov_3.229567"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..2145)
                     /locus_tag="PLN02_03285"
     CDS             complement(88..2145)
                     /locus_tag="PLN02_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51374.1"
                     /translation="MNLPRLTLLAASLALAFSSVHAEETTLSTVNVSAKGYAAADLET
                     PIATSSLERDEIARRGAANVGDALRGEPGIAVANDSGQGQNPIIRGLGKESIVLLVDG
                     MRLNSAQPAGAIASFMTLGLAERVEVVRGAASTLYGTGALGGAINVLLPQAKFTPGVG
                     VEAATSYDSASQGWRGSAVLNASQGDHALMLGASLARIDDYDTPHGKVERSGYDSNAF
                     IGQYRFRIDSRQQLRVSAQVHRDEDVWYPGSTRPHKSPLVGTTTVHSPEQERRLYELG
                     YQRKGDGERPLNLDLRVYRQDMERQIRSYSNNLQRDIVVNSVTFRTDGLDAKADWLVH
                     PQHLLSFGINAWEMSGNPDRSMASAAPFTTLSANNPFQDAEIRAFGAYLQDDMRFGKL
                     NLLAGLRYDTVKADAASMNNGARTSDLDGKDSALSGSLGAIYEIAPLLRPYASYARAF
                     RAPGMRERYESGLRGDGYYYAGSPEVEAEKADQFEIGLKGASRGVDYRVAAFYNRIDN
                     YITGQILSGAAASAACGAANAANCKKTVNLGSATIKGLEASVRWQVAAGHWLNAGYSR
                     IRGENDDLDEALFQMPADEISLGWQGAVLPGVKADFTLRLVDEQKRVATRFTNGAENR
                     TAGFVTADLGATWQYAKNQSLRLAVKNLADKAYHEHLTEGLSNQEIQAPGRSVQLAWR
                     GSF"
     gene            2338..2754
                     /locus_tag="PLN02_03290"
     CDS             2338..2754
                     /locus_tag="PLN02_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51375.1"
                     /translation="MKKSLIAAAFALAAGVAQAEGQAVTVIVNSASTMSQGVALVLAT
                     QMQAQGGQVHILLCDKAGDLALKGAGGETLKPQNVTPAQMLDGAMKKGATASVCALYL
                     PNSGHTPDQLKDGVTAAKPDAMGKALLEPQRKVVVF"
     gene            complement(2869..3288)
                     /locus_tag="PLN02_03295"
     CDS             complement(2869..3288)
                     /locus_tag="PLN02_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51376.1"
                     /translation="MKIHLLTLILVASLNHSAFAHDHPVEDLLELPHPLRSLVQGELA
                     QTLNDDQKAQVQTLRSEIQPQFLALMGEALPIQKRLQAAASDYDNRNPPSAADLERLA
                     RIRIRMSEIMAGAYNRLKVILDAPSWQTLQADLAHRR"
     gene            complement(3285..3752)
                     /locus_tag="PLN02_03300"
     CDS             complement(3285..3752)
                     /locus_tag="PLN02_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51377.1"
                     /translation="MLAPLKNRAILEREAAPDNTPSNPLLAENLALRHALRQARSSRK
                     RLLTLHAARLAALDERLNALLSERDALRLRLEQLESGQAIIELGQQLMAAREAEQQLM
                     AAIQRVWQLDKTLCAAHRECERLAGERDRLARCLCHRPADAGYPTVKQAEPRD"
     gene            complement(3736..3939)
                     /locus_tag="PLN02_03305"
     CDS             complement(3736..3939)
                     /locus_tag="PLN02_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51378.1"
                     /translation="MPNSNLWAATLSLVLIHEETGCRQSALHAARLLDHLCDLEIDDE
                     TRQLCERASLRLGDPEKVHACAA"
     gene            complement(4202..4444)
                     /locus_tag="PLN02_03310"
     CDS             complement(4202..4444)
                     /locus_tag="PLN02_03310"
                     /inference="COORDINATES: protein motif:HMM:NF022103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HOI51379.1"
                     /translation="MEQNKQTLRIPFLQATGPRGTLHLNRDKTPVSSREATDVCGARC
                     ISSDSLFGNCNEICIRHCGADYRLRITRQDKLILTK"
BASE COUNT          710 a         1321 c         1598 g          904 t
ORIGIN      
        1 gcaccaccag cggcagcttg ccggcggcgg cggccggtcc ggccagccag ccggcgagcg
       61 ccagggcggc gacgagcagg cgccgcatca gaaactcccg cgccaggcga gctggacgct
      121 gcggcccggc gcctggattt cctggttgga gaggccttcg gtcaggtgtt cgtggtaggc
      181 cttgtcggcg aggttcttga cggccaggcg caggctctgg tttttcgcgt actgccaggt
      241 ggcgccgagg tcggcggtga cgaagccggc cgtccggttt tccgcaccgt tggtgaagcg
      301 ggtggccacc cgcttctgtt cgtcgaccag gcgcagcgtg aagtcggcct tgacgccggg
      361 tagcacggcg ccttgccagc cgagcgagat ttcgtcggcg ggcatctgga acagcgcttc
      421 gtcgagatcg tcgttctcgc cgcggatgcg cgagtagccg gcgttcagcc agtggccggc
      481 ggcgacctgc cagcggacgc tggcttcgag gcccttgatc gtcgcgctgc ccagattcac
      541 cgttttcttg caattcgccg cgttggccgc gccgcaggcg gcgctggcgg cggcaccgct
      601 gaggatctgg ccggtgatgt agttgtcgat ccggttgtag aaggcggcga cgcgatagtc
      661 gacgccacgg ctggcgcctt tgaggccgat ttcgaattgg tcggctttct cggcttcgac
      721 ttccgggctg cccgcatagt aataaccatc gccgcgcagg ccggattcat aacgctcgcg
      781 catgcccggt gcccggaagg cgcgggcgta actggcgtag gggcgcagca gcggggcgat
      841 ttcgtagatg gcgccgagac tgccggaaag cgcgctgtcc ttgccgtcaa gatcgctcgt
      901 gcgggcgccg ttgttcatgc tggcggcgtc ggccttgacc gtgtcgtagc gcaggccggc
      961 aaggaggttc agcttgccga aacgcatgtc gtcttgcagg taggcgccaa aggcgcggat
     1021 ttccgcgtcc tgaaaaggat tgttggcgct caaggtggtg aacggcgcag cgctggccat
     1081 gctgcgatcc gggttgccgc tcatttccca ggcgttgatg ccgaacgaga gcaggtgttg
     1141 cggatgcacc agccagtcgg ccttggcgtc gagcccgtcg gtgcggaagg tgacgctgtt
     1201 gacgacgatg tcccgttgca gattgttgga ataggagcgg atttggcgtt ccatgtcctg
     1261 gcggtagacg cgcaggtcga ggttgagcgg tcgttcgccg tcgcccttgc gctggtaacc
     1321 gagttcatag agacggcgtt cctgttccgg cgagtgcacc gtcgttgtgc caaccagcgg
     1381 acttttgtgc gggcgcgtcg aacccggata ccagacatcc tcgtcccggt gcacttgcgc
     1441 cgagacgcgc aactgctgcc ggctgtcgat gcggaaacgg tactggccga tgaaggcgtt
     1501 cgaatcgtag ccgctgcgct cgaccttgcc gtggggcgtg tcgtaatcgt cgatgcgggc
     1561 cagcgaggcg cccagcatca aggcatggtc gccctggctg gcgttgagca cggcgctgcc
     1621 gcgccagccc tggctggcgc tgtcgtagct cgttgcggct tcgacgccga cgccgggggt
     1681 gaatttcgcc tgcggcagca acacgttgat cgcgccgccc agggcgccgg tgccgtaaag
     1741 cgtcgatgcc gcgccgcgca cgacttcgac ccgctcggcc aggcccagtg tcatgaagga
     1801 ggcgatggcg ccggccggtt gcgccgaatt gaggcgcatg ccatcgacca gcagaacgat
     1861 gctttccttg cccaggccgc ggatgatcgg gttctgtcct tgcccgctgt cgttggcgac
     1921 ggcgatgccg ggctcgccgc gcagggcgtc gccgacattg gcggcgcccc ggcgggcgat
     1981 ttcgtcgcgc tcgagcgagc tggtggcgat tggcgtctcc aggtcggcag cggcatagcc
     2041 cttggcgctg acattgaccg tggacagtgt ggtttcttcg gcatggaccg aggaaaaggc
     2101 gagggcaagg gatgcggcaa gcagggtgag gcgcggcagg ttcatgactg actccagtgg
     2161 aagggctggc tggcgtcgag ctggcttgcc ggaacgaaaa ctgcctgcat tttatcagtt
     2221 gagaatggtt cgcaatttaa ttcaaatcaa aaaatcattt tattagtaag ttctaatata
     2281 ctgccggtgt gccgctcgct gcggtcccgc tttttaaccc ctgacaggag tccttccatg
     2341 aaaaaatccc tcattgctgc cgctttcgct ctcgccgccg gtgtcgccca ggccgaaggg
     2401 caggcagtca ccgtgatcgt caattccgcc agtacgatga gccagggcgt cgctctggta
     2461 ctggcgaccc agatgcaggc ccaggggggc caggtgcata tcctgctgtg cgacaaggcc
     2521 ggcgatctgg cgctcaaggg cgctggcggc gaaaccctca agccgcagaa cgtgacgccg
     2581 gcgcagatgc tcgacggcgc gatgaagaag ggtgcgaccg cttcggtttg cgcgctctac
     2641 ctgccgaaca gcggccatac gccggaccag ctgaaggatg gcgtcaccgc cgccaagccg
     2701 gacgcgatgg gcaaggcgct gcttgaaccg caacgcaagg tcgttgtttt ctaagcggcc
     2761 tcgctgcatg aaaaatggcg ctggcttcgg tcagcgccat ttttttcaga agcgccagtc
     2821 gtcgtccggc ttgagctcgc gtgcgcatgc cggatggcgg cgaagcgttc agcgtcgatg
     2881 tgccagatcg gcctgcaacg tctgccagct cggggcgtcc aggatcactt tgagacggtt
     2941 gtaggcgcca gccatgatct cgctcatccg gatgcggatc ctggcgaggc gttcgaggtc
     3001 ggcggctgac ggcggattgc ggttgtcgta atcgctggct gccgcttgca gccttttctg
     3061 gatcggtagc gcttcgccca tcagggccag gaactgtggc tgaatctcgc tgcgtagtgt
     3121 ctggacctgg gctttctggt cgtcattgag cgtttgcgcc agttctccct gaaccaggga
     3181 gcgtagcggg tggggaagtt ccagcaggtc ctcgaccggg tggtcatgcg cgaaggcgct
     3241 gtggttcagg ctggcgacga ggatcagggt cagtagatgg attttcaatc gcggggctcc
     3301 gcttgcttga cggtggggta tccggcgtcc gccggccgat ggcacaggca gcgggcgagc
     3361 cggtcgcgtt cgccggccag tcgctcgcat tcgcgatgcg cggcgcacag ggttttgtcg
     3421 agttgccaga cgcgctgtat ggcggccatc agttgttgct cggcctcgcg cgccgccatc
     3481 aactgttgcc cgagttcgat gatggcctga cccgattcga gctgttcgag gcgcaggcgc
     3541 agggcgtcgc gctcgctcag cagcgcgttc aggcgctcgt ccagtgcggc cagacgggcg
     3601 gcgtgcaggg tcagcagacg cttgcggctg ctgcgtgcct ggcgcagcgc atggcgcagg
     3661 gccaggttct cggcgagcag gggattgctc ggtgtgttgt ccggcgccgc ctcgcgttcg
     3721 aggatggcac ggttcttaag cggcgcaagc atgcactttc tccggatcgc cgaggcgcag
     3781 gctggcgcgc tcgcacagtt ggcgtgtctc gtcgtcgatc tccagatcgc agagatggtc
     3841 gagcagacgg gcggcatgca gggccgactg ccggcaaccg gtttcttcat gaatcaggac
     3901 cagactcagg gtggcggccc acagattgct gttcggcatg gtattcctcc tcaggctgcg
     3961 gcgctggcga tcatcagcca gagcaggagc gcggcgatgg cggtattgat caatagactg
     4021 ttcatgatgt cacctccata attgagaact gatctcaata taccgattgc ggaaaattca
     4081 gtcaaataag aattgtttct atttgtgcct acggaattgg gtggagggcg ccggcgtgtg
     4141 atcggcgccc tccggtggag caggcggggt cagcgacctg ctccggcttg ctcgggcttg
     4201 cttacttggt caggatcagt ttgtcctggc gagtgatgcg caggcggtaa tcggcgccgc
     4261 agtgacggat gcagatttcg ttgcagttgc cgaacaggct atcgctggaa atgcagcggg
     4321 cgccgcagac gtcggtggct tcgcggcttg agacgggcgt cttgtcgcga ttcaggtgca
     4381 gcgtgccacg cggaccagtg gcctgaagga agggaatccg cagggtctgc ttgttctgtt
     4441 ccatggggag ttcctttcag agtcttacat ccgggtggcg taatactcga cgacgcgctg
     4501 caggtcgagc gggaaggggg cggcgtcgcc gcc
//
LOCUS       DAOSDX010000104         4526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6741_length_4526_cov_4.105122, whole genome shotgun sequence.
ACCESSION   DAOSDX010000104 DAOSDX010000000
VERSION     DAOSDX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6741_length_4526_cov_4.105122"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PLN02_03315"
     CDS             <1..1436
                     /locus_tag="PLN02_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOI51380.1"
                     /translation="LLHYDPELESDIAAAAALIAELAPHPVLAARGLAILFLGNDDEV
                     EAWLRERAGADYPRLENLRQTARANADGDLPTLLARERTAAADALATAVTRRAVNSSP
                     LLSQRVGQAVVHPVWGIPILLGVLYAVYQFVGVFGATTLVGLFEEDLFEGILNPAFTQ
                     LVENAAGGTWVSDLLVGQYGLWTMGMTYALALILPIVTTFFIAFGVLEDSGYLPRLTV
                     IANRLFAMIGLNGRAVLPMVLGLGCVTMATLTTRILHSPRERLITIFLLALAIPCSAQ
                     LGVVLGMLGGFSFTAVLVWALAMIGVLLLSGFLAARPIPGRRIPLVTELPPMRLPIAG
                     NVLKKTGGRLKWYLIEVIPLFLIGTFLMFALDRFGILPAIVEAGEPLVTGWLGLPKEA
                     SAAFVMGFLRRDFGATGLFAMADQLSPIQAVVGMITITLFIPCFASLLMMVKEQGIRV
                     AATMVAIIVPFAFFIGGLFNFALRALW"
     gene            1444..1686
                     /locus_tag="PLN02_03320"
     CDS             1444..1686
                     /locus_tag="PLN02_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOI51381.1"
                     /translation="MSPQHDARLPLAFIPPGNEVRLAALGQEIDPLQREQLTAYGLAE
                     NRPIRVLQQKPMTVILADEVELALEHAVARHIWVVR"
     gene            complement(1703..1960)
                     /locus_tag="PLN02_03325"
     CDS             complement(1703..1960)
                     /locus_tag="PLN02_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51382.1"
                     /translation="MKQGKSMELTAEQLVARTMYLLSRAATVGVCAGRVRALIQHLEC
                     VASDETLDPAIRSTSRDLIADWRAAQVEQFGEAALAQHVTH"
     gene            complement(1957..2499)
                     /locus_tag="PLN02_03330"
     CDS             complement(1957..2499)
                     /locus_tag="PLN02_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOI51383.1"
                     /translation="MARIGIFFGSTSGKTRKVAKMIKKRFDDATMADPVNVNRASADD
                     LAAYDFLILGTPTMGQGQLPGLSADCDEESWEEALPRFAGIDFTGRTVAIFGLGDQVS
                     YANEFLDAMIEIYDFVTERGARVVGSWPTEGYEFNTSQAVLDGRFVGLALDLDNQSEQ
                     TTARVESWLQQIAPDFGLAL"
     gene            2844..3329
                     /gene="bfr"
                     /locus_tag="PLN02_03335"
     CDS             2844..3329
                     /gene="bfr"
                     /locus_tag="PLN02_03335"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOI51384.1"
                     /translation="MKGDKKIIDILNDLLAGELTAVDQYLIHGEMYADMGLNELAEKS
                     IHESDHERQHARALIQRILFLEGKPDLSKRDALKIGKNVPDMLKADLDVEYKVDGDLK
                     KAIAACEKAQDYVSRDMLAAQLEDTEVDHAYFLEKQLRLIDLVGLPNYLQSQMGSGSP
                     A"
     gene            3340..3822
                     /gene="bfr"
                     /locus_tag="PLN02_03340"
     CDS             3340..3822
                     /gene="bfr"
                     /locus_tag="PLN02_03340"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOI51385.1"
                     /translation="MKGDKKVIEILNKVLKNELTSINQYFLHARMFRNWGLEKLNDYQ
                     YKQSIRVMKEADELIERILFLEGLPNLQNLGKLLIGENVVECLAGDLKYEQTIQRPLL
                     IEAIALCEEAQDYVSRELLEDLLEHCEKAIDWLETQQSLITDVTLPNYLQAHMEPGEG
                     "
     gene            4019..4222
                     /locus_tag="PLN02_03345"
     CDS             4019..4222
                     /locus_tag="PLN02_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOI51386.1"
                     /translation="MYICVCHAVTDRQIRQAAADGARTLKDLRRELGVTRDCGRCASC
                     AHECLKEAQDKGGKPGKVALMAA"
     gene            complement(4291..>4526)
                     /locus_tag="PLN02_03350"
     CDS             complement(4291..>4526)
                     /locus_tag="PLN02_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI51387.1"
                     /translation="TTKHREAALRFLEYLASDEAQRYFANGNNEWPAVPTVKVANEEL
                     AELGSFKVDTTPLQNIAKQLPAAQKLLDRVGYR"
BASE COUNT          858 a         1554 c         1278 g          836 t
ORIGIN      
        1 gcctgttgca ctacgatccc gaactggaaa gcgacatcgc cgccgccgcc gcgctgatcg
       61 ccgagctggc gccgcacccg gtgctcgccg cccgcggcct ggccatcctc ttcctcggca
      121 acgacgacga agtcgaagcc tggctgcgcg aacgcgccgg cgccgactac ccccgcctcg
      181 aaaacctgcg ccagaccgcc cgcgccaatg ccgacggcga cctgccgacg ctgctcgccc
      241 gcgaacgcac cgccgccgcc gacgcgctgg caacggcggt cacccgacgc gccgtgaaca
      301 gcagcccgct gctctcgcaa cgggtcggcc aggccgtcgt ccatccggtc tggggcattc
      361 ccatcctgct cggcgtgctc tacgcggtct accagttcgt cggcgtcttc ggcgcaacga
      421 cgctggtcgg gctgttcgag gaagacctct tcgaaggcat cctcaacccg gccttcaccc
      481 agctcgtcga aaacgctgcc ggcggcacct gggtcagcga cctgctggtc ggccagtacg
      541 gcctgtggac gatgggcatg acctacgcgc tggcgctgat cctgccgatc gtcaccacct
      601 tcttcatcgc tttcggcgtg cttgaggact ccggctacct gccccgcctg acggtgatcg
      661 ccaatcgcct gttcgccatg atcggcctca acggccgcgc cgtgctgccg atggtgctcg
      721 gtctcggctg cgtgacgatg gcgacgctga ccacgcgcat cctgcactcg ccacgcgaac
      781 gcctgatcac catcttcctg ctggcgctgg ccattccctg ctcggcccag ctcggcgtcg
      841 tcctcggcat gcttggcggc ttctccttca ccgcagtgct ggtctgggcc ctggccatga
      901 tcggcgtcct gctgctttcc ggtttcctcg ccgcccgccc gattcccggc cggcgcatcc
      961 cgctggtcac cgagctgccg ccgatgcgcc tgccgatcgc cggcaacgtc ctcaagaaaa
     1021 ccggcggccg cctcaagtgg tacctgatcg aggtcattcc gctgttcctg atcggcacct
     1081 tcctgatgtt cgcgctcgac cgcttcggca tcctgcctgc catcgtcgaa gccggcgaac
     1141 cgctggtcac cggctggctc ggcctgccca aggaagcctc ggcggccttc gtgatgggct
     1201 tcctgcgccg cgacttcggc gccaccggcc tgttcgcgat ggccgaccag ctctcgccga
     1261 tccaggccgt cgtcggcatg atcaccatca ccctgttcat tccctgcttc gccagcctgt
     1321 tgatgatggt caaggaacag ggcatccgcg ttgccgcgac gatggtcgcc atcatcgtcc
     1381 ccttcgcctt cttcattggc ggcctgttca acttcgcgct gcgcgccctc tggtaaaccg
     1441 atcatgagcc ctcaacacga tgcccgtctg ccgctcgcct tcattccacc cggcaatgaa
     1501 gtccggctgg cagccctggg ccaggaaatc gacccgctgc aacgggaaca actgaccgcc
     1561 tacggccttg ccgaaaaccg cccgatccgc gtcctccagc aaaaaccgat gaccgtgatc
     1621 cttgccgacg aagtcgaact ggcgctcgaa cacgccgtcg cccgccacat ctgggtcgtc
     1681 agataagcca ggcgatgccg gctcagtgcg tgacgtgctg cgccagcgcc gcctcgccga
     1741 actgctcgac ctgcgccgcc cgccagtcgg cgatcaggtc gcgcgaggtc gagcggattg
     1801 ccggatccag tgtctcgtcg ctggcaacgc attcgagatg ctggatcaag gcgcgtaccc
     1861 gccccgcaca aacgcccacg gtcgccgcgc gcgacaacag gtacatggtg cgtgccacca
     1921 gttgctccgc cgtcagttcc atgcttttcc cctgcttcat agcgctagtc cgaaatcggg
     1981 tgcgatctgt tgcaaccagg actcgacgcg cgccgtggtc tgctcggact ggttgtcgag
     2041 atcgagtgcc aagccgacaa aacgcccgtc gagcacggcc tgcgaggtat tgaactcgta
     2101 gccttcggtc ggccatgagc cgacgacacg agcgccgcgc tcggtgacga aatcgtagat
     2161 ctcgatcatt gcgtcgagaa actcgttggc atagctcacc tggtcgccga gcccgaaaat
     2221 ggccaccgtc ctgcccgtga aatcgatgcc ggcaaagcgc ggcaaggcct cttcccagct
     2281 ctcttcatcg caatcggcgc tgagccccgg caactgcccc tgtcccatgg tcggcgtgcc
     2341 gaggatgagg aaatcgtagg cggcgagatc gtcggctgac gcgcggttaa cgttgaccgg
     2401 atcggccatc gtcgcgtcgt cgaagcgttt cttgatcatc tttgccacct tgcgggtctt
     2461 gccgctggtg ctgccgaaaa agataccaat tctggccatg cttgaatctc ctgaagattg
     2521 gacaagacct taccaagcgc aaggtttatt aatgataaca attcccatcc gcaaatcaac
     2581 cccgtcacgg atgacttctc ctgcgaaatc accgccattc gtcgacggcg cctgctccgc
     2641 aaaatcaaac ctgcccggga ttgaagatcg gtgtgccaag cagcacacca ataaaacaaa
     2701 tgcgaattgt tcgtaattcg ttgaaaacgc agcatttttt ttgaacaaag caactcttcc
     2761 gcggtcgcag ctttacaatc cggcacacgg caagccagcc gcgccatgcc agccagccat
     2821 ttcataactt gcggagaaat gccatgaagg gcgacaagaa aatcatcgac atcctgaacg
     2881 acctgctggc cggcgaactg actgccgtcg accagtacct gatccacggc gagatgtacg
     2941 ccgacatggg tctcaacgaa ctggccgaga agtcgatcca cgaatccgac cacgaacgcc
     3001 agcacgcccg cgcgctgatc cagcgtatcc tcttcctcga aggcaagccg gacctgtcga
     3061 agcgcgacgc actgaaaatc ggcaaaaacg ttcccgacat gctgaaggcc gaccttgacg
     3121 tcgagtacaa ggtcgatggc gacctcaaga aggcgatcgc cgcctgcgag aaggcccagg
     3181 actacgtttc ccgcgacatg ctggcggcgc agctggaaga caccgaagtc gaccacgcct
     3241 acttcctcga aaagcagttg cgcctgatcg acctggtcgg cctgcccaac tacctgcaga
     3301 gccagatggg ctcgggcagc ccggcctgag gagcgcgaca tgaaaggcga caagaaggtc
     3361 atcgaaatcc tcaacaaggt cctcaagaac gagctgacct cgatcaacca gtatttcctg
     3421 cacgcccgga tgttccgcaa ctggggtctg gaaaagctca acgactacca gtacaagcag
     3481 tcgatccgcg tcatgaagga agccgacgag ttgatcgagc gcatcctctt cctcgaaggc
     3541 ctgcccaacc tgcagaacct cggcaagctg ctgatcggcg agaatgtcgt cgagtgcctg
     3601 gccggcgacc tcaagtacga gcaaaccatc cagcgcccgc tgctgatcga ggcgatcgcc
     3661 ctgtgcgagg aagcccagga ctacgtcagc cgcgaattgc tcgaagacct gctcgaacac
     3721 tgcgaaaagg cgatcgactg gctggaaacc cagcaatcgc tgatcactga cgtgaccctg
     3781 ccgaactacc tgcaggcaca catggaaccg ggcgaaggct gattcacgcc ccactcgtca
     3841 gcaagccgcg gccggtccgc ggcttttttt tgttgaccgc cactggcacc gtatttgcgt
     3901 atgaccttcc cgcaaggaga agtttgtcgc gcttgccaca aggcaaaaac gagtattgac
     3961 attcgttctc aactgcatac aataggaatt gttcgcatta cctttcaagg tgcctttgat
     4021 gtacatctgt gtctgccatg ccgtcaccga tcgccagatt cgccaggctg ccgccgacgg
     4081 cgcgcgcacc ctgaaagacc tgcgccgtga gctgggcgtc acccgcgact gcggtcgctg
     4141 cgcctcctgc gcccacgaat gcctgaagga agcccaggac aagggcggca agccgggtaa
     4201 ggtggccctg atggccgcct gaaacggaca ccggcccaac aaaaacggga acccgcgggt
     4261 tcccgttttt gttgcccgaa gtgcccttgc tcagcgataa ccgacgcgat cgagcagttt
     4321 ctgggcggcc ggcagttgct tggcgatgtt ctgcagcggc gtcgtatcga ccttgaagct
     4381 gcccagctcg gcgagttcct cgttcgccac cttgaccgtc ggcaccgccg gccactcgtt
     4441 gttgccgttg gcgaaatagc gttgcgcctc gtcgctcgcc aggtattcga ggaaacgcag
     4501 cgccgcctcg cggtgtttcg tggtct
//
LOCUS       DAOSDX010000105         4526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6743_length_4526_cov_3.243346, whole genome shotgun sequence.
ACCESSION   DAOSDX010000105 DAOSDX010000000
VERSION     DAOSDX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6743_length_4526_cov_3.243346"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /gene="glnA"
                     /locus_tag="PLN02_03355"
     CDS             <1..229
                     /gene="glnA"
                     /locus_tag="PLN02_03355"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289488.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOI51388.1"
                     /translation="KNLYDLPPEEDAKIPTVCASLEEALENLDKDREFLTRGGVFSND
                     WIDAYIALKMEEVNKIRMTTHPVEFDLYYSC"
     gene            342..791
                     /locus_tag="PLN02_03360"
     CDS             342..791
                     /locus_tag="PLN02_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOI51389.1"
                     /translation="MTRKLLILSLALAPLALSAQTIYKCTDAGGGVLISNSRVNKNCQ
                     AIVSEEHNTIPAPRSAPRAAGNPTPAGFPRVQEDTQKARDGDRRLILEQELAGEQRKL
                     EQARKELAEQEAVRTGNPAERTAPYRDRVAQHERNLQAIQKELANLR"
     gene            complement(800..3949)
                     /locus_tag="PLN02_03365"
     CDS             complement(800..3949)
                     /locus_tag="PLN02_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI51390.1"
                     /translation="MSQRFNLSDWTLHHRTLVGYFMLALALMGIVAYGRLGQAEDPPF
                     TFKAMVIQAYWPGATAQQVEQQLTERIEKKLQEMPSVDRLTSYSRAGEATVIFFAKDS
                     YRSAQMPEIFYQVRKKIGDIRHTLPEGIQGPFFNDEFGDVFGNLFAVTGEGLDYPLLK
                     DQAERIRDALLPVKGVGKIDIFGIQDEKIFVDLDNAKLATLGIAPTAIGQALAQQNAV
                     NGAGFFEMDDERIQLRPSGAFSSEQAVADTLIRSNNRSFRLGDIATVHRGTSEPPGIQ
                     VRFAGKPALVIGVAMAPGGDIIALGKALEQKLASLRPTLPVGVDIHEAVSQPAAVTRS
                     IDAFVKALAEAVIVVLAVTFISLGLRTGMVVAVSIPLVLAATFLAMHSLDIGLHKVSL
                     GALVLALGLLVDDAIIAVEMMWVKMEQGWERTRAASFAFTSTAGPMLSGTLVTVAGFI
                     PIALAKSSTGEYAFAFFQINAIALLISWLAAVIAIPWLGYKLLPDPRAAQTPGRLAAR
                     WPRLDRLARRLGAYGPAHPVDHDVYGTPFYQRFRRFVGFCVDHRWLVIGVTLLLFVLA
                     IFGMGKVQKQFFPNSTRLELNVELRLQEGASLTATADAARALENWLEADRASHDDFSH
                     YVSWIGSGVPRYFLSLDRQLPASNVAQFIIETRDMAAREALRERLIAHYRQAPDGVRA
                     NVARIENGPPVGYPVQYRVAGEDIAALRRIAEEVASIMRSDERLSNVNFDWHELSKTI
                     AVDIDQDKARLLGVSSQDVAALLAMSLQGYSVSALREGSKRIEVVLRGGDKERHDPAA
                     LAGLMLPTPAGRSVPLSQIARLTPVFEPGLVWRRDRVPTITVRANLYGTTQPATIVDH
                     LQGPIDSLRQRLPAGFSIVPGGSVEESAKGGNSVGAGLPLFILAVVTILMIQLQNVSR
                     VVMVLLTAPLGMIGITLFLLVFNQPFGFMALLGTIALFGMVMRNSVILVDQIEQDLAS
                     GRARCEAIVESTVRRLRPIVLTAAAAVLAMIPLSRNDFFGPMAIAIMGGLIVATALTL
                     FFLPALYAAWYKVGKTAPA"
     gene            complement(3951..>4526)
                     /locus_tag="PLN02_03370"
     CDS             complement(3951..>4526)
                     /locus_tag="PLN02_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI51391.1"
                     /translation="ADFPGVVAAVLADAGQVVGAGQAVLRIARPEEKEVLVAVPEGRV
                     AALRQAEKITVRLWAAPELAIAGKLRELGAVADPQTRTYPARISLLEAPPQAMLGMTA
                     SVSLDAAPVASVVVPLAAVGDNGQGPFVWVVEADKLRRVPVRVGSFRADGAEIIGGLR
                     GGEAVVIAGISQLLEGQAVTARPAPAPAAQR"
BASE COUNT          771 a         1465 c         1583 g          707 t
ORIGIN      
        1 caagaacctc tacgacctgc cgccggaaga ggatgccaag atcccgaccg tctgcgcttc
       61 gctcgaggaa gccctggaaa acctggacaa ggatcgcgag ttcctgaccc gtggcggtgt
      121 cttctccaac gactggatcg atgcctacat cgccctgaag atggaagaag tgaacaagat
      181 ccgcatgacc acgcacccgg tcgagttcga tctctactat tcctgctgat atctcgccgg
      241 aatcaggaag ggcgggggtt tccccgccct ttttttgttc acttttcggc aagtcgctcg
      301 tttactccgg tacactgacc cggtttccag gaagcatgac gatgacacgc aagctgctaa
      361 ttctctccct ggcgctggca ccgctggcgc tgtcggccca gaccatctac aagtgcaccg
      421 atgccggcgg cggcgtgctg atctccaaca gccgggtcaa caagaactgc caggcgatcg
      481 tcagcgaaga gcacaacacg atcccggcgc cgcgcagcgc cccgcgcgcc gccggtaacc
      541 cgaccccggc gggtttcccg cgtgtccagg aagataccca gaaagcgcgt gatggcgacc
      601 gtcgcctgat tctcgaacag gaactggccg gcgagcaacg caagctggag caggcgcgca
      661 aggaactggc cgaacaggaa gccgtgcgca ccggcaatcc ggccgaacgg acggcaccct
      721 accgcgaccg cgttgcccag cacgagcgca acctgcaggc gatccagaag gaactggcga
      781 acctgcgctg agccttcgct caggcgggcg cggtttttcc gaccttgtac caggcggcgt
      841 agagcgccgg caggaagaac agcgtcagcg cggtggcgac gatcaggccg cccatgatgg
      901 cgatcgccat cggcccgaag aaatcgttgc gcgacagcgg gatcatcgcc agtaccgccg
      961 ctgctgcggt cagcacgatc ggccgcaggc ggcgcaccgt cgattcgacg atcgcttcac
     1021 agcgggcgcg gccgctcgcc aggtcctgct cgatctggtc gaccaggatt accgaattgc
     1081 gcatcaccat gccgaacagg gcgattgtcc cgagcagcgc catgaagccg aagggctggt
     1141 tgaagacgag caggaagagg gtgatgccga tcatcccgag cggtgcggtg agcagcacca
     1201 tgacgacgcg cgagacattc tgcaactgga tcatcaggat ggtgacgact gccaggatga
     1261 acaagggcag gccggcgccg accgagttgc cgcccttggc cgactcctcg accgagccgc
     1321 cgggaacgat gctgaaaccg gcgggcagcc tttggcgcag gctgtcgatg gggccctgca
     1381 gatggtcgac gatggtcgcc ggttgcgtcg tgccgtagag attggcgcgc acggtgatgg
     1441 tcggcacgcg gtcgcggcgc cagaccaggc cgggttcgaa gaccggcgtc aggcgcgcga
     1501 tctgggacag cggcacgctg cgcccggcgg gcgtcggcag catcaggccg gcgagggcgg
     1561 ccgggtcgtg gcgttccttg tcgccgccgc gcaggacaac ctcgatgcgc ttgctgcctt
     1621 cgcgcagtgc gctgacgctg tagccctgca gcgacatggc gagcagcgcg gcgacgtcct
     1681 ggctggaaac gccgagcagg cgcgccttgt cctggtcgat gtcgacggcg atggtcttcg
     1741 acagctcgtg ccagtcgaaa ttgacgttcg atagccgttc gtcgctgcgc atgatgctcg
     1801 ccacttcctc ggcgatgcgc cgcagtgcgg caatgtcctc gccggccacc cggtactgca
     1861 ccgggtaacc caccggcggc ccgttctcga tccgcgcgac gttggcgcga accccgtccg
     1921 gcgcctgccg gtaatgggcg atcaggcgtt cgcgcagggc ttcacgggcc gccatgtcgc
     1981 gcgtctcgat gatgaactgg gcgacgttgc tggccggcaa ctggcggtcg agcgacagga
     2041 aatagcgggg gacgccgctg ccgatccagg agacgtaatg ggagaaatcg tcgtggctgg
     2101 cgcggtcggc ctccagccag ttttccagag cccgcgcggc gtccgcggtg gcggttagcg
     2161 aggcgccttc ctgcaggcgc agttcgacgt tgagttcgag tcgggtcgag ttggggaaga
     2221 actgcttctg caccttgccc ataccgaaga tggcgagcac gaacagcagc agcgtgacgc
     2281 cgatcaccag ccagcgatga tcgacgcaga agccgacgaa gcgccggaag cgctggtaga
     2341 acggcgtgcc gtaaacgtcg tggtcgaccg gatgggccgg tccgtaggcg ccgagccggc
     2401 gtgccaggcg gtcgaggcgc ggccagcgcg cggccaggcg gccgggcgtc tgcgccgcac
     2461 gcgggtcggg cagcagcttg tagccgagcc agggaatggc gatgacggcg gccagccagg
     2521 aaatcagcag ggcaatggcg ttgatctgga agaaggcgaa ggcgtattcg ccggtcgacg
     2581 acttggccag ggcgatcggg atgaagccgg cgacggtgac cagggtgccg gagagcatcg
     2641 ggccggcggt gctggtgaag gcgaacgagg cggcgcgggt gcgctcccag ccctgttcca
     2701 tcttcaccca catcatctcg acggcgatga tcgcgtcgtc taccaaaagg cccagcgcca
     2761 gcaccagcgc gcctagcgac accttgtgca ggccgatgtc gaggctgtgc atggcgagga
     2821 aggtggcggc taggaccagc gggatcgaga cggcgaccac catgccggtg cgcaggccga
     2881 ggctgatgaa ggtgacggcg aggacgacga tcaccgcctc ggccagggcc ttgacgaagg
     2941 cgtcgatcga gcgcgtcacg gccgccggct ggctgaccgc ctcgtggatg tcgacgccga
     3001 ccggcagcgt tggccgcagg ctggcgagct tctgctccag cgccttgccc agggcgatga
     3061 tgtcgccgcc cggcgccatg gcgacgccga tgaccagcgc cggcttgccg gcgaagcgga
     3121 cctggatgcc gggcggctcg ctggtgccgc ggtgcacggt agcgatgtcg ccgagccgga
     3181 agctgcggtt gttgctgcgg atcagggtat cggccactgc ctgctcgctg ctgaaggcgc
     3241 cgctcggccg caactggatg cgttcgtcgt ccatctcgaa gaaaccggcg ccgttgaccg
     3301 cgttctgctg cgccagcgcc tggccgatcg ccgtcggagc gatgcccagc gtcgccagct
     3361 tggcgttgtc gagatcgaca aagatcttct cgtcctggat gccgaagatg tcgatcttgc
     3421 cgacgccctt gaccggcagc agcgcgtcac ggatgcgttc ggcctggtcc ttgagcagcg
     3481 gatagtcgag gccttcgccg gtgaccgcga acaggttgcc gaaaacgtcg ccgaactcgt
     3541 cgttgaagaa gggcccctgg atgccttcgg gcagggtgtg gcggatgtcg ccgatcttct
     3601 tgcgcacctg atagaagatt tccggcatct gcgccgaacg gtagctgtcc ttggcgaaaa
     3661 agatcaccgt cgcttcgccg gcgcgcgaat aactggtcag gcgatcgacg gagggcatct
     3721 cctgcagctt cttctcgatc cgctcggtca gctgctgttc cacttgctgc gcggtggcgc
     3781 ccggccagta ggcctggatc accatcgcct tgaaggtgaa cggcgggtct tccgcctggc
     3841 cgagccggcc gtaggcgaca atgcccatca gggcgagggc gagcatgaag tagccgacca
     3901 gcgtccggtg atgcagtgtc cagtcggaca ggttgaagcg ctggctcatc tcagcgctgc
     3961 gcggccggtg ccggggcggg gcgggcggtc accgcctggc cctcgagcag ctgcgagatg
     4021 ccggcgatca ccacggcttc gccgccgcgc aggccgccga tgatctcggc accgtcggcg
     4081 cggaagctgc cgacgcgtac cggcacccgg cgcagcttgt cggcctcgac gacccagacg
     4141 aaaggcccct ggccgttgtc gccgaccgcc gccagcggca ccaccacgct ggcgaccggt
     4201 gccgcatcga ggctgacgct ggccgtcatg cccagcatcg cctgcggcgg cgcttcaagc
     4261 aggctgatgc gggccggata ggtgcgtgtc tgcggatcgg cgacggcgcc cagttcgcgc
     4321 agtttgccgg cgatcgccag ctcgggcgcc gcccacaggc ggacggtgat tttttcggcc
     4381 tggcgcagcg cggcgacgcg gccttccggc acggcgacca ggacttcctt ttcttccggg
     4441 cgggcgatgc gcagcaccgc ttggccggcg ccgaccacct ggcccgcgtc ggcgaggacg
     4501 gcggcgacga cgcccgggaa atcggc
//
LOCUS       DAOSDX010000106         4526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6745_length_4526_cov_3.119436, whole genome shotgun sequence.
ACCESSION   DAOSDX010000106 DAOSDX010000000
VERSION     DAOSDX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6745_length_4526_cov_3.119436"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLN02_03375"
     CDS             complement(<1..823)
                     /locus_tag="PLN02_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA--succinate CoA transferase"
                     /protein_id="HOI51392.1"
                     /translation="MQTNNASSRILCPHLRGKIMSAEEAAALIPSGVNVGMSGFTGAG
                     YPKAVPSTLAKRIMDANLHGKKFKIGVWTGASTAPELDGALAMVDGVEMRLPYQSDPT
                     CRKRINAGEMEYIDIHLSHVAQFVWGGFLGKLDVAVVEVAGILEDGRLIPSSSVGNNK
                     TWLDQADKIILEVNAWQNPALEGMHDIYYGTALPPHRKPIPIVKTDDRIGEPYYRIDP
                     NKVIAVVETNKADRNSPFTPPDANSKLIAGHILDFLQHEVKKGRLPANLLPLQSGV"
     gene            1103..2040
                     /gene="gcvA"
                     /locus_tag="PLN02_03380"
                     /pseudo
     CDS             1103..2040
                     /gene="gcvA"
                     /locus_tag="PLN02_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993025.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
     gene            2114..2392
                     /locus_tag="PLN02_03385"
     CDS             2114..2392
                     /locus_tag="PLN02_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51393.1"
                     /translation="MLATRRFALVLLLCLAQLGALLHELGHARDDVGAAPESCSICLA
                     AHALAAALPPALLAIAALPVAFPPPTPALETALRAEAPQPTQRGPPTA"
     gene            2469..3872
                     /locus_tag="PLN02_03390"
     CDS             2469..3872
                     /locus_tag="PLN02_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51394.1"
                     /translation="MLQKPIALALLAAFSCAAPAAEDAELAAIRSQLAELKKNYEQRI
                     AALEDKLAQSRAQAEKPVAVAAATEAPRLGTPAGGFNPEISLTLQGQYKSMKNIDERG
                     IAGFWPAGGHDHESEGIKGISKRGFSVDHTELMLAANVDPYWRGQAILAALDGEVEVE
                     EAWFQSLGLDHGIGLKGGRFLSGIGYQNEQHPHAWDFASAPLMYQALFGEHGSYAQDG
                     VQFKWLAPTPIWLEFGAEAGRGANFPGTDRNQNRAGAGALFVHLGDDVGSSHSWRAGL
                     SYLHTRAREREAEVHDANDVHGDASFTGRSRTWLADFVWKWAPDGNPKYRNFKFQTEY
                     FHRTERGDIGCVGEEAGAACDGTRDSYRSRQSGWYAQAVYQFTPHWRAGLRHEQLDSG
                     SLRIGANVLAQLGDENQMFARYRPKRSALMLDYSWSEFSRVRLQYERDRSMPGVTDNQ
                     FTVQYIMSLGAHGAHKF"
     gene            3882..>4526
                     /locus_tag="PLN02_03395"
     CDS             3882..>4526
                     /locus_tag="PLN02_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOI51395.1"
                     /translation="MRRLIVLLAALASLPAWSAVEVFATVPEWGALAREIGGERVAVF
                     VATNALQDPHRIEARPSLLARARRAQLVVATGAELETGWLPVVLRESGNAAVQPGRDG
                     YFEAAALVERLEVPATLDRSHGDVHASGNPHVHLDPHRLLRIGEALAERLARVDPANA
                     AAYRTGFADFAGRWRSAIGRWEKAAAPLRGVPVLVHHGSFAYLTHWLGMPEIGTL"
BASE COUNT          702 a         1519 c         1529 g          776 t
ORIGIN      
        1 cgacgccgga ttgcagcggc agcaggttgg ccggcaggcg gcctttcttc acttcgtgct
       61 gcaggaaatc gaggatgtgg ccggcgatca gcttggagtt ggcatccggc ggggtaaacg
      121 gcgagttgcg gtcggccttg ttggtttcga cgacggcgat caccttgttc gggtcgatcc
      181 ggtaatacgg ttcgccgatc cggtcgtcgg tcttgacgat ggggatcggc ttgcggtgtg
      241 gcggcagcgc ggtgccgtag tagatatcgt gcatgccttc gagggccggg ttctgccagg
      301 cattcacttc gaggatgatc ttgtcggcct ggtccagcca ggtcttgttg ttgccgaccg
      361 acgatgatgg aatcagtcgc ccgtcttcca ggatgccggc gacttcgacg acggcaacgt
      421 caagcttgcc gaggaaaccg ccccagacga actgggcgac gtgcgacagg tggatgtcga
      481 tgtattccat ttcgccagcg ttgatccgct tgcggcaggt cgggtcggac tggtagggca
      541 ggcgcatctc gacgccgtcg accatggcca gtgcgccgtc gagttcgggc gcggtcgagg
      601 cgccggtcca cacgccgatc ttgaatttct tgccatgcag gttggcatcc atgatgcgct
      661 tggccagcgt ggacggtacc gccttcggat agccggcgcc ggtgaagccg ctcatgccga
      721 cgttgacgcc ggaaggaatc agcgcggcgg cctcttcggc ggacatgatc ttgccgcgca
      781 ggtggggaca aaggatgcgg gaggaagcgt tgtttgtctg catgttgttt gttggtcgtc
      841 aggtacgaat agccaaaaaa aagcccgcac ggggcgggct caagcccaca actcaagggc
      901 gcgtgatgct cagaggagac tgaggcatga ggttgaaacc ggcagtgcgc caatctggcg
      961 cactgccggc tccggaatgc tgtgccgccc acgggggcgt ccctgaatgt cgaaaactcg
     1021 atggggcgca acaatacgga tcggcttgcc gggcgacaaa cgacacttta tgatgcgatg
     1081 gaatagaaaa acttaatcgt ccgtggccta tcgtttacct cccctgtcgg ccttgcgcgc
     1141 tttcgaagcg gcagcccgcc atctcagctt caagctggcg gcggaggaac tgcacgtgac
     1201 gccggccgcc gtcagccagc agatccgcct gctcgaggac tatctgggcg taccgctgtt
     1261 tcgccgcctg acccgggcga tcgaactgac cgcgaccggc gccgccatgc tgcccgatct
     1321 gcgccgcggc ttcgattgcc tggcggcggc tgtggacggc acgcgcagcg gcgacgacgg
     1381 cgtgttgacc atccacgccc cacccagctt cgcctcgggc tggtgccgcg cttgccgcgc
     1441 tttgccgccg cccacccggg catcgccctg cgtctgtcga gcggcgacga taccgtcgac
     1501 cgcgacggcg acaccggctg gatcagcccg ctcgatccgc gtagcgccga ctgcgaggtg
     1561 gccatccgct acggccgcgg cgattatccc ggcctgcgcg tcgagaagat attttcgccg
     1621 gactatgtgc cggtgtgcag tccgcgcctg ctcgcagccg gacggccgct gacgacgccg
     1681 gacgacttgc gccaccacct gctgatccac gacgagacga tcagcgacga ggacctgcca
     1741 ccgggttggc agcaatggct ggcgcaggcc ggcgtgcgcg gtgtcgatgc cgggcgcggc
     1801 ctgcgtttct ctaacgccat cctggccgtc gaggcggcgc tcgacgggca gggcgtcgcc
     1861 ctcgcgctgt cgccgctggt cgaggccgat gttgccgccg gccgcctgat ccggcctttc
     1921 gcactgagca tcccgtcgcc ctacgcctac tggctggcga tgccggaaat gctcgccggc
     1981 cgggctgcgg ttgccgcctt ccgggcctgg atcctgaccg aaggggcggc tggcgcctga
     2041 atccggcgcg atcggctggt gaggtctgtt acaaaatgca ataatataac attgcatttt
     2101 caccgccgcc gccttgctcg ccacgcgccg ttttgccctc gtcctgttgc tttgtctggc
     2161 ccaactcggc gcgctgctgc acgagctcgg ccatgcccgg gacgatgtcg gcgcggcgcc
     2221 ggagagctgc agcatctgcc tggccgccca tgcgctggcg gcggcgctac cccctgcgct
     2281 gttggcgatc gccgcgttgc ccgtggcttt tccgccgccg acaccggcgc tcgagacggc
     2341 gctgcgagct gaagcgccgc agccgacaca gcgcggaccg ccgaccgcct gaaatcccct
     2401 tcccgtgttt cgccgtctgc tgtcctgtcc ggtcagcggc ggccccgtgt cgatttcagg
     2461 agtctcccat gttgcagaaa cccatcgccc tggccttgct ggccgccttt tcctgtgccg
     2521 cgccagcggc cgaggatgcc gaactggccg ccatccgcag ccagctcgcc gaactgaaga
     2581 agaattacga gcagcgcatc gccgcgctcg aagacaagct ggcgcagagc cgcgcccagg
     2641 cggagaaacc cgtcgctgtc gcggcggcga ccgaggcgcc gcgcctcggt actccggccg
     2701 gcggcttcaa ccccgagatc tcgctgaccc tgcaggggca gtacaagtcg atgaagaaca
     2761 tcgacgagcg ggggatcgcc ggtttctggc cggctggcgg tcacgaccac gaaagcgaag
     2821 ggatcaaggg catttcgaag cgcggtttct cggtcgacca caccgagctg atgctggccg
     2881 ccaacgtcga tccctactgg cgcggccagg cgatcctggc ggcgctcgac ggcgaggtcg
     2941 aagtcgagga agcctggttc cagtcgctcg gcctcgacca cggcatcggc ctcaagggcg
     3001 ggcgtttcct gtccggcatc ggctaccaga acgagcagca tccgcatgcc tgggacttcg
     3061 ccagcgcgcc gctgatgtac caggcactgt tcggcgagca cggcagctac gcgcaggacg
     3121 gggtgcagtt caagtggctg gcgccgacgc cgatctggct cgaattcggc gccgaagccg
     3181 ggcgcggcgc caatttcccg ggcaccgacc gcaaccagaa ccgcgctggt gccggcgcct
     3241 tgttcgtaca cctgggtgat gatgtcggca gctcgcatag ctggcgcgcc ggtttgtcct
     3301 acctgcatac ccgcgcccgg gagcgcgagg cggaggtgca tgacgccaac gacgtgcatg
     3361 gcgacgccag cttcaccggc cgtagtcgga cctggctggc cgatttcgtc tggaaatggg
     3421 caccggacgg caacccgaag taccgcaact tcaagttcca gaccgagtat ttccatcgca
     3481 cggaacgcgg cgacatcggt tgtgtcggcg aggaagccgg tgccgcctgc gacggcacgc
     3541 gcgacagcta ccgcagccgg caatccggct ggtacgcgca ggcggtctac cagttcacgc
     3601 cgcactggcg tgccggcctg cgccacgagc agctcgattc gggcagcctg cgcatcggcg
     3661 ccaacgtact tgcccagctc ggcgacgaga accagatgtt cgcccgctac cggccgaagc
     3721 gcagcgcgct gatgctcgat tacagctgga gcgagttttc ccgcgttcgc ctgcagtacg
     3781 aacgcgaccg ttcgatgccc ggcgtcaccg acaaccagtt caccgtccag tacatcatga
     3841 gcctcggtgc ccatggtgcg cacaagttct gaggaaacgc catgcgcaga ttgatcgtat
     3901 tgctcgccgc cctcgccagc ctgccggcct ggtcggcggt cgaagtgttc gcgacggtgc
     3961 cggagtgggg ggcgctggcg cgcgaaatcg gcggcgagcg cgtcgccgtc ttcgtcgcca
     4021 cgaacgcgct gcaggacccg caccggatcg aggcgcggcc gtcgctgctc gcccgcgccc
     4081 gccgagcgca actggtggtc gccaccggcg ccgaattgga aaccggctgg ttgccggtgg
     4141 tcctgcgcga atccggcaac gccgcggtgc agccggggcg cgacggctat ttcgaagcgg
     4201 cggcgctggt cgaacgcctg gaggtgccgg ccacgctcga ccgctcccac ggcgacgtgc
     4261 atgcctccgg caacccgcac gtccatctcg acccgcaccg tctgctgcgc atcggcgagg
     4321 cgctggccga gcggctggcg cgcgtcgatc cggcgaacgc cgccgcttac cgcaccggct
     4381 tcgccgattt cgccggccgc tggcggagcg cgattgggcg ctgggagaag gccgccgcgc
     4441 cgctgcgcgg ggtgccggtg ctggtccatc acggctcctt cgcctacctg acgcactggc
     4501 tgggcatgcc ggaaatcggc acgctc
//
LOCUS       DAOSDX010000107         4505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6793_length_4505_cov_3.137303, whole genome shotgun sequence.
ACCESSION   DAOSDX010000107 DAOSDX010000000
VERSION     DAOSDX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6793_length_4505_cov_3.137303"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLN02_03400"
     CDS             complement(<1..684)
                     /locus_tag="PLN02_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI51396.1"
                     /translation="MSEIDLKRFFLEQVPLFASFPADKVEEIVIKSRLATFEGNEAML
                     ETGDETRHFGIMISGHAEISNADNTGTRTVIAQLGPGDVFGVLSLLTGDRIAADVIAG
                     NRCFVLMVPQEVFASHILVNPKAVAYLSRLLAERTRAISVGELAADGQAKAGAGDLLS
                     LTTRQPGKVVALNVGISQIHFGIYDTEESGADIHGIIDNADQQHAQITIMVGPQRQTL
                     EHEPFKLSDL"
     gene            complement(802..1722)
                     /locus_tag="PLN02_03405"
     CDS             complement(802..1722)
                     /locus_tag="PLN02_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI51397.1"
                     /translation="MPLLLGVLFAIGLVAPYAVYPTFLMKILCYALFACAFNLLLGFV
                     GLLSFGHAAFLGTAGYVCGMVVRDVGLTPELGILAGTVAAGLLGWLFGWLAIKRTGIY
                     FAMITLALAQMVFFLCVQIKETGGEDGLQGVPRGHLLGVIDLSDNIAMYYLVYAIFCC
                     GFFVIYRTIHSPFGQILKAIRENEPRAISLGYDVTKFKLIAFTISAALAGTAGATKTL
                     VFQLASLTDVHWHASGEVVLMTLLGGLGTILGPIVGAATIVSLQTQLADSVGSWVTVI
                     MGVIFVLCVLAFRRGMVGELQALIKRSGVN"
     gene            complement(1775..2656)
                     /locus_tag="PLN02_03410"
     CDS             complement(1775..2656)
                     /locus_tag="PLN02_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI51398.1"
                     /translation="MEIFGIPTALLGSQLLVGLINGSFYAILSLGLAIIFGLLNIINF
                     CHGAQYMMGAFVAWIALTRFGINYWVALIAAPLIVGAVGVVIERLMLRRLYKLDHLYG
                     LLLTFGLALIIEGIFRDQYGISGESYDVPELLSSGINLGFMFLPIYRAWIIVASLTVC
                     FGTWYMIERTKLGAYLRAGTENSQMVQALGINVPLLITFTYGYGVALAAFAGVLAAPV
                     FQVNPLMGSNLIIVVFAVVVIGGMGSIMGSILTGLGLGLIEGLTKVFYPEASSVIVFV
                     IMVIVLLVRPAGLFGKT"
     gene            complement(2715..3929)
                     /locus_tag="PLN02_03415"
     CDS             complement(2715..3929)
                     /locus_tag="PLN02_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI51399.1"
                     /translation="MKTKQLAITACAAALATLSAGASAQISGGSVKIGVLTDLSGTYS
                     DLAGSGAVLATRMAIDDFIAREKPAFKIEMVTADHQNKADIASNKAREWIERENVDTV
                     TELVTTSTALAVMKVAKEKDRIAIMNGPASTPITNEQCNDVTVHYTYDTYALANGTAK
                     AVTTQGGKNWFFLTADYAFGHSLEKDSSAVVTANGGKVLGSVRHPFPGSDFSSFLLKA
                     QASGAQVIGLANAGADTTNAIKQAAEFGITPKQSLAGLLMFITDVHSLGLKPTQGMFL
                     TTGFYWDRNDETRAWSKRFFDKQKKMPTMVQAGQYSSIYHYLKAVKAAGSDDTGKVMA
                     MMKKTPINDFFAKNGQIRDDGRMVHDMYLVQVKKPADSKYPWDYYTIKATIPAAEAFQ
                     PLAQSRCPLIKK"
     gene            complement(3958..>4505)
                     /locus_tag="PLN02_03420"
     CDS             complement(3958..>4505)
                     /locus_tag="PLN02_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI51400.1"
                     /translation="LVGRRTGSIMISGNEVIHEPTHKIAHHGVGYCPEERAIFTSLST
                     EENLTLAPIISSGGMSVDELYQMFPNLEERRASPGGKLSGGEQQMLAMARILRTGAKL
                     LLLDEITEGLAPVIVKKLGEVIVELKRRGFTIVLVEQNFRFAAPLADRHYVLEHGEII
                     ATISHEELPGKMDWLHQTLGV"
BASE COUNT          794 a         1298 c         1598 g          815 t
ORIGIN      
        1 caggtcggac agcttgaacg gctcgtgttc cagcgtctgc cgctgcggcc cgaccatgat
       61 cgtgatctgc gcgtgctgct ggtcggcgtt gtcgatgatg ccgtggatgt cggcgccgct
      121 ttcctcggtg tcgtagatgc cgaagtggat ctggctgatg ccgacgttga gggcgacgac
      181 cttgcccggc tggcgggtgg tcagcgacaa caggtcgccg gcgccggcct tggcctgacc
      241 gtcggcggcg agttcgccaa cgctgatcgc ccgcgtccgt tcggcgagca gccgcgacag
      301 gtaggcgacc gccttgggat tgaccaggat atgcgaggcg aagacttcct gcggcaccat
      361 cagcacgaag cagcggttgc cggcgatcac gtcggcggca atgcggtcgc cggtgagcaa
      421 ggagagcacg ccgaagacgt cgcccgggcc gagctgggcg atgaccgtgc gggtgccggt
      481 gttgtcggcg ttggaaatct cggcgtggcc gctgatcatg atgccgaaat ggcgcgtttc
      541 atcgccggtc tcgagcatcg cctcgttgcc ctcgaaggtg gcgagccggg atttgatgac
      601 gatttcctcg accttgtcgg ccgggaagct ggcgaacagg ggcacctgtt cgaggaagaa
      661 gcgtttcagg tcgatttcgc tcatgctgat cccaccattt tttgcctagt atagtgggcg
      721 aagactacca ttgtgttgcg gtgcagcata gaggggtttc cacaggtttt accggcagcc
      781 ggtgcgcggc taccggctgc ttcaattcac cccggatcgc ttgatcagtg cctgcagctc
      841 gcccaccatg ccgcgccgga aggcgagcac gcagagaacg aagatgacgc ccatgatcac
      901 cgtcacccag gagccgaccg aatcggccaa ctgggtctgc agggagacga tggtggcggc
      961 gccgacgatc ggtccgagga tggtgcccag gccgccgagc agggtcatca gcaccacttc
     1021 gccggaagcg tgccaatgca cgtcggtcag cgaggcgagc tggaacacca gggtcttggt
     1081 cgccccggcg gtgccggcca gcgcagccga gatggtgaag gcgatcagct tgaatttcgt
     1141 gacgtcgtaa ccgagcgaga tggcgcgcgg ttcgttttcg cggatcgcct tgaggatctg
     1201 gccgaacggc gaatggatgg tgcggtagat gacgaagaag ccgcagcaga agatggcata
     1261 caccaggtag tacatggcga tgttgtcgga aagatcgatg acgccgagca ggtggccacg
     1321 cggcacgccc tgcaggccgt cctcgccgcc ggtttccttg atctggacgc agaggaagaa
     1381 aaccatctgg gccagggcca gcgtgatcat cgcgaagtag atgccggtgc gcttgatcgc
     1441 cagccagccg aacagccagc cgagcagccc ggcggcgacg gtgccggcga ggatgccgag
     1501 ctccggcgtc aggccgacat cgcgcaccac catgccgcag acgtagccgg cggtgccgag
     1561 aaaggcggca tgaccgaagg agagcaggcc gacgaagccg agcaacaggt tgaaggcgca
     1621 ggcgaacagc gcgtagcaga ggattttcat caggaaggtc gggtacaccg cgtagggggc
     1681 gactaggccg atggcgaaga ggacgcccag cagcagcggc attttcgact cggggaaggc
     1741 ggcgggcgca ttcatgtgct tttcctttga cggctcaggt cttgccgaac agcccggccg
     1801 ggcgcaccag caggacgatg accatgatga cgaagacgat gacgctggac gcttccgggt
     1861 agaacacctt ggtcagcccc tcgatcaggc cgagaccgag cccggtgagg atcgagccca
     1921 tgatcgagcc catgccgccg atgacgacga cggcgaagac gacgatgatc aggttggagc
     1981 ccatcagcgg attgacctgg aagaccgggg cggccagtac gccggcaaag gcggcgagcg
     2041 ccacgccgta gccgtaggtg aaggtgatga gcagcggcac gttgatgccc agcgcctgca
     2101 ccatctgcga attctcggtg cctgcgcgca ggtaggcgcc gagcttggtg cgctcgatca
     2161 tgtaccaggt gccgaagcag acggtgagcg aggcgacgat gatccaggcg cggtagatgg
     2221 ggaggaacat gaaaccgagg ttgatgccac tggacagcag ttcgggaacg tcgtagcttt
     2281 cgccggagat gccgtactgg tcgcggaaga tgccttcgat gatgagcgcc aggccgaagg
     2341 tgaggagcag gccgtagagg tggtcgagct tgtacaaccg gcgcagcatc aggcgttcga
     2401 tgacgacgcc gacggcgccg acgatcagcg gcgcggcgat cagcgccacc cagtagttga
     2461 tgccgaagcg ggtcagcgcg atccaggcga cgaaggcgcc catcatgtac tgcgcgccgt
     2521 ggcagaagtt gatgatgttg agcagaccga agatgatcgc cagcccgagg ctgaggatgg
     2581 cgtagaacga gccgttgatc agcccgacca gcagctggct gccgagcagg gcggtgggga
     2641 tgccgaagat ttccatgggg cgctccaggc ccgttgccga gcggcgccgg caacgggcat
     2701 tgcgatggct gctcttattt cttgatcagc gggcaacgcg actgcgccag cggctggaag
     2761 gcctcggccg ccgggatcgt cgccttgatc gtgtagtagt cccacggata cttggagtcg
     2821 gccggcttct tgacctgcac caggtacatg tcgtgcacca tccggccgtc gtcgcggatc
     2881 tgcccgttct tggcgaagaa atcgttgatc ggcgtcttct tcatcatcgc catcaccttg
     2941 ccggtgtcgt cgctgcccgc cgccttgacc gccttcaggt agtggtagat cgacgaatac
     3001 tggccggcct ggaccatggt cggcatcttc ttctgcttgt cgaagaagcg cttcgaccag
     3061 gcgcgggttt cgtcattgcg gtcccagtag aagccggtgg tcaggaacat gccctgggtc
     3121 ggcttgaggc cgagcgaatg gacgtcggtg atgaacatca ggaggccggc cagcgactgc
     3181 ttgggcgtga tgccgaattc ggccgcttgc ttgatcgcgt tggtggtgtc ggcgccggcg
     3241 ttggccaggc cgatgacctg ggcgcccgag gcctgcgcct tgagcaggaa ggacgagaaa
     3301 tcggaaccgg ggaaggggtg gcgcaccgag cccagtacct tgccgccgtt ggcggtgacc
     3361 acggccgacg aatccttttc cagcgagtgg ccgaaggcgt agtcggcggt caggaagaac
     3421 cagttcttgc cgccctgggt ggtgaccgcc ttggccgtgc cgttggccag cgcgtaggtg
     3481 tcgtaggtgt agtgcacggt gacgtcgttg cactgttcgt tggtgatcgg tgtcgaggcc
     3541 gggccattca tgatggcgat gcggtccttc tccttggcca ccttcatcac cgccagcgcg
     3601 gtcgaggtgg tgaccagctc ggtgacggtg tcgacgtttt cgcgctcgat ccactcgcgc
     3661 gccttgttgg aggcgatgtc ggccttgttc tggtggtcgg cggtgaccat ctcgatcttg
     3721 aaagcgggtt tttccctggc gatgaagtcg tcgatggcca tgcgggtggc cagcacggcg
     3781 ccggagccgg ccaggtccga ataggtgccg gacaagtcgg tgagtacgcc gattttgacc
     3841 gaaccgcccg aaatctgggc cgaagctccg gcggacaggg tggccagcgc ggcggcgcag
     3901 gcggtaatgg caagttgctt ggttttcatg ggtgtctcct cctggtgggc ggctcggcta
     3961 gacgccgagt gtctggtgca gccagtccat cttcccgggg agttcctcgt ggctgatggt
     4021 ggcgatgatc tctccgtgtt cgagcacgta atggcggtcg gccagcggcg ccgcgaaacg
     4081 gaagttctgt tcgacaagga cgatggtgaa gccgcgtcgc ttcagttcga cgatcacctc
     4141 gcccagcttc ttgacgatga ccggggccag gccttcggtg atctcgtcga gcagcagcag
     4201 cttggcgccg gtgcgcagga tgcgcgccat ggccagcatc tgctgctcgc cgccggacag
     4261 cttgccgccc gggctggcgc ggcgttcctc gagattgggg aacatctggt acagctcgtc
     4321 gaccgacatg ccgccgctcg agatgatggg cgccagggtc aggttctcct cggtactgag
     4381 cgaggtgaag atggcgcgtt cctcggggca gtagccgacg ccgtggtggg cgatcttgtg
     4441 ggtgggttcg tggatcacct cgttgccgct gatcatgatc gagccggtgc gccggccgac
     4501 caggc
//
LOCUS       DAOSDX010000108         4499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6804_length_4499_cov_4.066832, whole genome shotgun sequence.
ACCESSION   DAOSDX010000108 DAOSDX010000000
VERSION     DAOSDX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6804_length_4499_cov_4.066832"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLN02_03425"
     CDS             <1..789
                     /locus_tag="PLN02_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOI51401.1"
                     /translation="RAIHDASGRRGPFVAVNCSALPESVFESELFGHEAGAFTGAGKR
                     RVGRIEHADGGTLFLDEIESMPLALQVKLLRVLQERSVERLGSNQSIAVDCRVVAASK
                     ADLKALADAGQFRADIYYRLNVVSIDLPPLRERRDDIPLLIAAFLQEAAQRYQRPPAS
                     WTPADLARWQAHDWPGNVRELKNVANRWALGLPDGLASPVSGPAASSLSAQVDAAERR
                     AIEAALQANAGNVAQAAEALQVPKKTLYDKLTRHGISAEGFRAH"
     gene            854..1444
                     /locus_tag="PLN02_03430"
     CDS             854..1444
                     /locus_tag="PLN02_03430"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HOI51402.1"
                     /translation="MSLDLLPSFIREHYEVHEWKHACAILKQDFPDEWQEIVGVLSDF
                     RLLKSWLTVGGGRKSKVAEAFDGALGKLGWQEKGFDTKMVVDGVSLDSPTHKVDCFKN
                     RVALEIEWNNKDPFFDRDLNNFRLLFDLRVVSVGIIVTRCDELQDIFDELGRGTSYGS
                     STTHIGKLLPRIQGGGGGGCPLLVFGIRKSLYEEDC"
     gene            1446..2108
                     /locus_tag="PLN02_03435"
     CDS             1446..2108
                     /locus_tag="PLN02_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HOI51403.1"
                     /translation="MQPTAAEDFKSSITGQFSTILADPPWQFQNRTGKMAPEHKRLSR
                     YPTLTLDDIKAIPVADAAAPASHLYLWVPNALLAEGLAVMQAWGFTYKSNIVWHKIRK
                     DGGPDGRGVGFYFRNVTELVLFGIRGSMRTLPPGRRQVNFLATQKREHSRKPDEFYDI
                     VESCSPGPYLELFARHRREGWQNWGNEVPETVGEHILSERKAAREAQLTLLQDTAPYS
                     VG"
     gene            2118..3131
                     /gene="murB"
                     /locus_tag="PLN02_03440"
     CDS             2118..3131
                     /gene="murB"
                     /locus_tag="PLN02_03440"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOI51404.1"
                     /translation="MQQLNTAIDLTPFNTLALPGRAAHYLKVTDPAQLAAVPPGERRF
                     VLGGGSNLVLTGDFPGLLLHMAIAGKRLLREDDKAFYIEAGAGENWHDFVQWTLAQGW
                     PGLENLSLIPGTVGAAPVQNIGAYGLEVGERLDSVTAWDFEKRVFFTLDREACRFAYR
                     DSLFKQRGWHLDGRIAIVSVVFRLAKAWQANIGYADLRQELAARGIAEPGASDVADAV
                     IAVRQRKLPDPALTPNAGSFFHNPVVERSVAEALKAQHPALPCYAQPDGRVKLAAGWL
                     IEQAGWKGKALGPVGMYEKQALVLVNRGGATGADVRRTMAAVQAAVREKFAIELSPEP
                     VFL"
     gene            complement(3106..3807)
                     /locus_tag="PLN02_03445"
     CDS             complement(3106..3807)
                     /locus_tag="PLN02_03445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOI51405.1"
                     /translation="MRLPILCLFLLSTANAFAAAIAVDVGHGGRDTGAISARGRAEFD
                     FNRDFAGVLADRLEAGGQQVRRVNFDGAIASLAARPEAAAGSDFFISIHHDSIGEAWL
                     LPWTWEGVERTHTEAKRGYGLFVSAANPDLPTSLLCASTMGAMLRRAGFVPSDWHGRR
                     HVAADARNGVWYYDNLVVLYRTTLPAVLFEAGVIKHKDEELELLDPARQARMADALAT
                     GLAACLQVKEIPAPD"
     gene            3869..4261
                     /locus_tag="PLN02_03450"
     CDS             3869..4261
                     /locus_tag="PLN02_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI51406.1"
                     /translation="MPTPPPSRIAMPELTIFSRQSPAPEYDRPRPERLLEGNPLRTTH
                     EHFAAPRGDLVAGVWACEPGAWRIAFGENKDEFFCIIEGRIRIADNEGNSAEFGPGEA
                     GVIPAGFSGSFTVLEAVRKHYVVLERAD"
     gene            4301..>4499
                     /locus_tag="PLN02_03455"
     CDS             4301..>4499
                     /locus_tag="PLN02_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51407.1"
                     /translation="MTEKTLPPRGTSAHNPDLNWSQVRETVLMLELAAVQIEHAMKDS
                     NASVDVLTRSFTAMAGCMQEIA"
BASE COUNT          817 a         1539 c         1435 g          708 t
ORIGIN      
        1 cgcgccatcc acgacgccag cggccggcgc gggcctttcg tcgcggtcaa ctgctcggcg
       61 ctaccggaaa gcgtcttcga gagcgaactc ttcggccacg aagcgggtgc cttcaccggc
      121 gccggcaagc ggcgcgtcgg ccgcatcgaa cacgccgacg gcggcacgct cttcctcgac
      181 gagatcgagt cgatgccgct cgccctccag gtcaagctgc tgcgcgtgct gcaggaacgc
      241 agcgtcgaac gcctgggcag caaccagagc atcgccgtcg attgccgcgt cgtcgccgcc
      301 agcaaggccg acctcaaggc gctggccgac gccggccagt tccgcgccga catctactac
      361 cgcctcaacg tcgtcagcat cgacctgccg ccgctgcgcg aacggcgcga cgacattccg
      421 ctgctcatcg ccgccttcct ccaggaagcc gcccagcgct accagcgccc accggccagc
      481 tggacgccgg ccgacctggc gcgctggcag gcgcacgact ggccgggcaa cgtgcgcgaa
      541 ctgaagaacg tcgccaaccg ctgggcgctc ggcctgcccg acggcctcgc cagtcccgtt
      601 agcggtccgg cggccagttc gctgagcgcc caggtcgacg ccgccgagcg acgcgccatc
      661 gaagcggcgc tgcaggccaa cgccggcaac gtcgcccagg cagccgaagc cctgcaggtg
      721 ccgaagaaga cgctctacga caagctcacc cgccacggca tcagcgccga aggcttccgg
      781 gcacactgac cctgcggcgc tttttcccct tccaggccga cgccatgctc cggccggctg
      841 atagaatggc gccatgagtc ttgacctcct gccctccttc atccgcgagc attacgaagt
      901 gcacgaatgg aaacacgcgt gcgccatcct gaagcaggat tttccggacg aatggcagga
      961 aatcgtcggt gtgctgagcg atttccgttt gttgaaaagc tggcttaccg ttggcggcgg
     1021 ccgtaaatcc aaagttgccg aagccttcga tggcgccctc ggcaaactcg gctggcagga
     1081 gaaaggcttc gacaccaaga tggtggttga tggggtcagc ctcgattcac cgacccacaa
     1141 ggtcgactgc ttcaagaacc gggttgcgct ggaaatcgag tggaacaaca aggacccgtt
     1201 ctttgaccgg gatctcaaca atttccgcct actcttcgat ctgcgcgtag ttagcgtcgg
     1261 catcatcgtg acccgctgcg acgaactgca ggacattttc gacgaactgg gccgcggcac
     1321 gtcgtatggt tcctcgacaa cgcacatagg caaactgctc ccccgcatcc agggtggtgg
     1381 cggtggcgga tgccccttgc tcgtcttcgg catccgcaaa tcactttacg aggaggactg
     1441 ctgagatgca gccgacggca gcagaagatt tcaagtcctc gatcaccggg caattcagca
     1501 ccatcctggc tgatccgccc tggcagttcc agaaccggac cggcaagatg gcgccggaac
     1561 acaagcgcct ttcccgttac ccaacgttga cgctggacga tatcaaggca attccggtcg
     1621 ccgacgctgc cgcccccgcc agccacctct acctgtgggt gccgaatgcg ctgctggccg
     1681 aagggttggc ggtgatgcag gcctggggat tcacctacaa atcgaacatc gtctggcaca
     1741 aaatccgcaa ggacggcggt ccggacggac gcggtgtcgg tttctacttc cgcaacgtca
     1801 ccgaactggt gctcttcggc attcgcggca gcatgcgcac gctgccgccc ggacgccgcc
     1861 aggtcaattt cctcgccacg caaaaacgcg agcattcgcg caaaccggac gaattctacg
     1921 acatcgtgga atcctgtagc cccggcccct acttggaact gttcgcccgg catcggcgcg
     1981 aaggctggca gaactggggt aacgaagttc cggaaaccgt cggcgagcac attttgagcg
     2041 aacgcaaagc tgctcgcgaa gcccaactga cgctgcttca ggatactgcg ccttacagtg
     2101 tcggttgatt cgggcagatg cagcagctta acaccgccat cgacctcacc cccttcaaca
     2161 ccctcgccct gcccggccgc gcggcgcatt acctgaaggt gaccgatccg gcccaactgg
     2221 ctgcggtgcc gccgggcgag cgccgtttcg tcctcggcgg cggcagcaac ctggtgctta
     2281 ccggcgattt tccggggctg ttgctgcaca tggcgatcgc cggcaagcgg ctgttgcgtg
     2341 aggacgacaa ggccttctac atcgaggccg gcgctggcga gaactggcac gacttcgtgc
     2401 aatggacgct ggcacaaggc tggcccgggc tggaaaacct gtcgctgatc ccgggcacgg
     2461 tcggtgctgc gccggtgcag aacatcggcg cctacgggct ggaagtcggc gagcgcctgg
     2521 attcggtgac ggcctgggat ttcgagaaac gggtgttttt cacgctcgac cgcgaggcct
     2581 gccgcttcgc ctaccgcgac agcctgttca agcaacgggg ctggcacctt gacgggcgga
     2641 tcgccatcgt ctcggtcgtc ttccggctgg ccaaggcctg gcaggcgaac atcggctacg
     2701 ccgacctgcg ccaggaactc gccgcccgcg gcatcgccga acccggcgcc agcgatgttg
     2761 ccgacgcggt gatcgccgtg cgccagcgca agctgcccga tccggcgctg acgccgaacg
     2821 ccggcagctt cttccacaac ccggtggtcg agcgcagcgt cgccgaggcg ctgaaagcac
     2881 agcatccggc gctgccctgt tacgcgcagc cggacggccg ggtcaagctg gccgccggtt
     2941 ggctgatcga gcaggctggc tggaagggca aggcgctcgg tccggtcggc atgtacgaga
     3001 agcaggcgct ggtcctggtc aatcggggcg gcgcgaccgg cgccgacgtg cggcggacga
     3061 tggccgcggt gcaggcggcg gtgcgcgaga aattcgctat cgaactcagt ccggagccgg
     3121 tatttctttg acttgcaggc aggcggccaa cccggtcgcc aaggcgtcgg ccatgcgcgc
     3181 ctgacgcgcc ggatcgagca gttccagctc ctcgtccttg tgcttgatga cgccggcctc
     3241 gaacagcacc gccggcagcg tcgtgcggta gaggacgacc aggttgtcgt agtaccagac
     3301 gccgttccgc gcgtcggccg cgacgtgccg gcggccgtgc cagtcgctcg gcacgaaacc
     3361 ggcgcgacgc agcatcgccc ccatcgtcga ggcgcagagc aggctggtcg gcagatccgg
     3421 attggccgcc gagacgaaca ggccgtagcc gcgcttggcc tcggtgtggg tgcgctcgac
     3481 gccttcccag gtccacggca gcagccaggc ctcgccgatc gaatcgtgat ggatggagat
     3541 gaagaagtcg ctaccggcgg ccgcctcggg acgcgccgcc aggctagcga tggcgccgtc
     3601 gaaattgacc cggcgcacct gctggccgcc ggcttcgaga cggtcggcga ggacaccggc
     3661 gaaatcgcgg ttgaagtcga attcggcgcg gccgcgcgcc gaaatggcgc cggtatcgcg
     3721 tccgccgtgg ccgacatcga cggcgatcgc ggcggcaaag gcgtttgcag tggacaacag
     3781 gaacaagcag agaatcggca ggcgcatgcg ccgattatcg cggcaaatcg cccgccggcc
     3841 cagcccgcga cgacaccctt ggcttaaaat gcctaccccg cccccgtccc gcatcgccat
     3901 gcccgaactg accatcttct cccgccagtc gcccgccccg gaatacgacc ggccacgccc
     3961 cgagcgcttg ctcgaaggca acccgctgcg caccacgcac gagcatttcg cggcgccgcg
     4021 gggcgacctg gtcgccggcg tctgggcctg cgaaccgggc gcctggcgca tcgccttcgg
     4081 cgagaacaag gacgagttct tctgcatcat cgaagggcgt atccggatcg ccgacaacga
     4141 aggcaacagc gccgaattcg gtcccggcga ggcgggcgtc attccggccg gattcagcgg
     4201 cagcttcacc gtcctcgaag ccgtgcgcaa gcactacgtg gtgctggaac gggctgactg
     4261 aattgcgcta tagtcggcgc caggccgaca ggaaagaaca atgaccgaga agaccctccc
     4321 gccgcgcggt acctccgccc acaatcccga cctgaactgg agccaggtcc gcgaaaccgt
     4381 cctcatgctc gaacttgccg cggtgcagat cgagcacgcg atgaaggaca gcaatgcctc
     4441 ggtcgacgtg ctgacccgct cattcacggc gatggccggc tgcatgcagg aaattgccg
//
LOCUS       DAOSDX010000109         4472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6857_length_4472_cov_2.733303, whole genome shotgun sequence.
ACCESSION   DAOSDX010000109 DAOSDX010000000
VERSION     DAOSDX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6857_length_4472_cov_2.733303"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="PLN02_03460"
     CDS             complement(<1..1272)
                     /locus_tag="PLN02_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51408.1"
                     /translation="MSAAMLRPFLAYLLRGRLAFPGGHRPPAGHTVPARFAGVGVAAS
                     PDPAVDDWIIATLRGAGIVNVRLDFTYGDADGPAGRLLGRLLDAGFHVVLHLLQPTDA
                     ARAMPDAAAGAAWREFVGATLDAFGERVAMIEACSTINRRRWAGYTPDGFFAAWDIAW
                     QEIRQRGLLLAGPSITDFEPPWSVGILAGLQARGRLSDIHTDNLFAERATEPERDDPK
                     VLGPRLAGLLRMNLVKKARLLQRIGADFGVPRLFSPAAFWTLPRIGRQLPATEQKQAD
                     YLTRYLVLAAGSGALEGAWWGPLICHREGLVDDGVSDYPALERITHYAAVDGALADFR
                     PRPALAALAAFNARIPGRRYRGCLCGGDGLEIHAFVGTDGALTHVAWTINGRAAALVD
                     VYDAATLVAASFHDRDGTALDEAPTLLTEMPV"
     gene            complement(1269..1862)
                     /locus_tag="PLN02_03465"
     CDS             complement(1269..1862)
                     /locus_tag="PLN02_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOI51409.1"
                     /translation="MKTLTQQDYQAMSEGARVLEADPHGAKVLLLADGTILKLFRRKR
                     LFTSAMIFPYATRFASNARKLAALGVPVPEVIDVLRIPELGRDAVHYRPLPGVTLRQL
                     AGEVLPEERKRRLREAFNRLVVLLHDNGVYFRSLHIGNVIVTPDDRLGLIDFSDMRIY
                     PWSLGTYLRRRNIQRLYKVATEIEWIDLPFILGKGRR"
     gene            1983..3770
                     /gene="msbA"
                     /locus_tag="PLN02_03470"
     CDS             1983..3770
                     /gene="msbA"
                     /locus_tag="PLN02_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOI51410.1"
                     /translation="MSHDSDPSASSLKIYLRLLSYVRPFVGHFAISLLGFAIFASSEP
                     MLASVLKYFVDGLSAPPETTHHDLPLIGSVQLMFGVPLLMLLIVAWRSVGSFLGNYFL
                     AKVSLGLINDLRQALFNSLLQLPNRYFDENNSGHLISRITYNVTMVTGAATDAIKVVV
                     REGLTVIFLFGYLLWMNWKLTAVMIVILPVIGAMVSRASRKFRKQSRKIQAAMGELTH
                     IASETIQGYRAVRSFGGEPYETRRFKAASEDNMRKQLRMVKTGATFTPALQLVSFSAM
                     AVLLTLVLYLRGDATAGDLVAYITAAGMLPKPIRQLSEVSATIQKGLAGAESIFAQLD
                     ELPEENRGTVEREKIAGRLEVRELSFSYPGSEQPVLRNISFAAEPGQMIALVGRSGSG
                     KSTLANLIPRFYHHDTGAILLDGVDVENYTLRNLRRHIALVSQQVTLFNDTVADNIAY
                     GDLAGCPREDIVAAARAGYADEFIARLPDGYDTLVGENGVLLSGGQRQRLAIARAILK
                     DAPLLILDEATSALDTESERHIQAALETATANRTTLVIAHRLSTIERADLILVMERGE
                     IVERGSHAELLALNGHYARLHAMQFSEMS"
     gene            complement(3767..>4472)
                     /locus_tag="PLN02_03475"
     CDS             complement(3767..>4472)
                     /locus_tag="PLN02_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOI51411.1"
                     /translation="EFLGKIGRECTQFAVERGIKRFAVVAREASELLRDFLSRPDTCL
                     DEGIRLKSGGTCTVAEIAAGGRCLVVKRYNLKHWRHALSRAWRPSRAWHSWREAHRLG
                     FYGIATPRPLAVLEERFGPLRGRAFLVTEHCAGRSLLDSLDPQQPPSPAVAEALQTLF
                     AELRALRITHGDLKASNLLWEDGRVVLIDLDAMCQHRSEGAFVRAWRRDRARFLRNWP
                     AESVLYRWLDERLPAA"
BASE COUNT          804 a         1534 c         1434 g          700 t
ORIGIN      
        1 gacgggcatt tcggtcaaca gcgtcggcgc ttcgtcgagc gccgtaccgt cacggtcgtg
       61 aaagctggcg gcaaccagcg tcgccgcatc gtaaacgtcg accagcgccg ccgcccggcc
      121 gttgatggtc cacgcgacat gggtcagggc gccgtcggtg ccgacaaagg catggatctc
      181 cagaccgtcg ccgccgcaca ggcaaccgcg gtagcgccgc cccggaatgc gggcattgaa
      241 agcggccagg gcggccagcg ccggtcgcgg gcggaagtcg gccagcgccc cgtcgaccgc
      301 ggcgtaatga gtgatccgct cgagcgccgg gtagtcgctc acgccgtcgt ccaccagtcc
      361 ctcgcggtgg cagatcagcg gcccccacca cgccccctcg agcgcccccg agccggcggc
      421 gaggaccagg tagcgggtca ggtaatcggc ctgtttctgc tcggttgccg gcagctggcg
      481 gccgatcctt ggcagggtcc agaaggcggc cggtgagaac agccggggta cgccgaagtc
      541 ggcgccgatg cgctgcagca ggcgtgcctt cttgaccagg ttcatgcgca gcaggccggc
      601 cagccgggga ccgaggacct tcgggtcgtc gcgctccggc tcggtcgccc gctcggcgaa
      661 caggttgtcg gtatggatgt cggacaggcg gccgcgggcc tgcaagccgg ccaggatgcc
      721 gacactccac ggtggttcga agtcggtgat gctcggcccg gcgagcagca ggccgcgctg
      781 ccggatttcc tgccaggcga tgtcccaggc ggcgaaaaaa ccgtccggcg tatagccggc
      841 ccagcgtcgg cggttgatcg tcgaacaggc ttcgatcatc gccacccgct caccgaaagc
      901 atccagcgtg gcaccgacga actctcgcca ggctgcgccg gcggcggcat cgggcatcgc
      961 ccgcgcggca tcggtcggct gcagcaggtg gaggacgaca tggaaaccgg cgtcgagcag
     1021 gcgaccgagc aggcgcccgg ccggtccgtc ggcatcgccg taggtgaagt cgagtcggac
     1081 gttgacgatg ccggccccgc gcagggtggc gatgatccag tcgtcaaccg ccgggtcggg
     1141 cgaagcggca acgccgacgc cggcgaatcg tgccgggacg gtgtgcccgg ccggcggccg
     1201 gtgcccgccc gggaaagcga gacggccgcg cagcagatag gccaggaaag gccgcaacat
     1261 ggccgcactc atcgccgtcc cttgccgaga atgaacggca ggtcgatcca ttcgatctcc
     1321 gtcgccacct tgtacaggcg ctggatgttg cggcggcgca gataggtgcc gagcgaccac
     1381 ggataaatgc gcatgtcgga aaagtcgatc aggccgagcc ggtcgtccgg cgtgacgatg
     1441 acgttgccga tgtgcagcga acggaaatag accccgttgt cgtgcagcag cacgaccagc
     1501 cggttgaatg cttcgcgcag gcgacgcttg cgctcttccg gcaagacctc accggcaagc
     1561 tggcgcaggg tgacaccggg cagcggacga tagtgcacgg cgtcgcggcc aagttcggga
     1621 atacgcagga cgtcgatcac ctccggcact ggcacgccga gcgcggccag cttacgggca
     1681 ttgctggcaa agcgcgttgc ataggggaag atcattgccg aggtgaacag gcgcttgcgc
     1741 cggaacagct tgaggatcgt cccgtcggcc aggagcagca ccttggcgcc gtgcggatca
     1801 gcctcaagta cgcgtgcgcc ttcgctcatc gcctgatagt cctgctgcgt cagcgttttc
     1861 aatgccaatt acccggagaa atcgggggat tttagcatcg aaggtgcaac aggttcgtca
     1921 tggcgcacat ggtacactcg cgcccacttt ctttccgcgc gtcgccccac gatcgccaag
     1981 ccatgtcgca cgacagtgac ccgtccgctt cctcactgaa aatctacctc cggctgctgt
     2041 cctacgtccg cccctttgtc ggacactttg ccatcagcct gctcggcttc gccatcttcg
     2101 cctcgtcgga accgatgctg gcctcggtac tcaagtactt cgtcgacggc ctgagcgcgc
     2161 cgccggaaac cacgcaccac gacctgccgc tgatcggcag cgtgcaactg atgttcggtg
     2221 ttccgctgct gatgctgctg atcgtcgcct ggcgtagcgt cggctccttc ctcggcaact
     2281 acttcctggc caaggtttcc ttgggcctga tcaacgacct gcggcaggcc ttgttcaaca
     2341 gcctcctgca actgccgaac cgctatttcg acgagaacaa ctccggccac ctgatctcgc
     2401 gcatcaccta caacgtcacc atggtcaccg gcgccgccac cgacgccatc aaggtcgtcg
     2461 tccgcgaagg cctgaccgtg atcttcctgt tcggttacct gctgtggatg aactggaaac
     2521 tgaccgcggt gatgatcgtc atcctgcctg tcatcggcgc catggtcagc cgcgccagcc
     2581 gcaagttccg caagcagagc cgaaagatcc aggcggcgat gggcgaactg acgcatatcg
     2641 cctcggaaac catccagggc taccgcgctg tccgcagttt cggcggcgaa ccttacgaaa
     2701 ccagacgctt caaggcggcc agcgaggaca acatgcgcaa gcagctgcgc atggtgaaga
     2761 ccggcgccac cttcacgccg gcgctgcaac tggtctcctt ctcggcgatg gctgtcctgc
     2821 tgacgctggt cctctacctg cgcggcgacg ccacagcagg cgacctggtc gcctacatca
     2881 ccgctgccgg catgctgccg aagccgatcc gccagttgtc ggaagtcagc gcgacgatcc
     2941 agaagggcct ggccggcgcc gaaagcatct tcgcccagct tgacgaactg ccggaggaaa
     3001 accgcggcac cgtcgaacgg gagaagatcg ccggccggct tgaggtgcgc gagctgagct
     3061 tcagctatcc cggcagtgag cagccggtgt tgcgcaatat cagcttcgcc gccgaaccgg
     3121 gccagatgat cgctctggtc ggccgctccg gcagcggcaa atcgacactc gccaacctga
     3181 tcccgcgctt ctaccaccac gacaccggcg ccatcctgct cgacggcgtc gatgtcgaga
     3241 attacacgct gcgcaacctg cgccgccaca tcgccctggt cagccagcag gtgacgctgt
     3301 tcaacgatac cgtcgccgac aacatcgctt acggcgacct tgccggctgc ccgcgcgaag
     3361 acatcgtcgc cgccgcccgc gccggctacg ccgacgagtt cattgcccgc ctgcccgacg
     3421 gctacgacac gctggtcggt gaaaacggcg tgctgctctc gggcggccag cggcaacgcc
     3481 tggcgatcgc ccgcgccatc ctcaaggatg cgcccttgct gatcctcgac gaagcgacct
     3541 cggccctcga caccgaatcc gagcgccaca tccaggccgc gctggaaacc gccaccgcca
     3601 accggacgac gctggtcatc gcccaccgcc tgtcgaccat cgaacgcgcc gacctgatcc
     3661 tggtcatgga gcgaggagaa atcgtcgaac gcggcagcca cgccgaactg ctcgcattga
     3721 acggccatta cgcccgtctg cacgcgatgc agttcagcga aatgagttag gcggcgggca
     3781 agcgctcatc gagccagcga taaagcaccg actcggccgg ccaattgcgc aggaaacgcg
     3841 cccggtcgcg ccgccaggcg cggacgaaag cgccctccga gcgatgctgg cacatcgcgt
     3901 cgaggtcgat caggacgacc cgcccatctt cccagagcag attgctcgcc ttgaggtcgc
     3961 cgtgcgtgat gcgcaaggca cgcagttctg cgaagagcgt ctgtaacgct tcggccacgg
     4021 ccggcgacgg tggctgttga gggtcgagac tgtcgagcag gctgcgcccg gcgcaatgct
     4081 cggtcaccag gaaggcccgg ccgcgcagcg gaccgaaacg ttcctcgagc accgccagcg
     4141 ggcgtggcgt ggcgatgccg tagaaaccca gacgatgcgc ctcgcgccac gaatgccagg
     4201 cccggctcgg tcgccaggcg cgcgacaggg cgtggcgcca gtgcttgagg ttgtaacgct
     4261 tgacgaccag gcaccgtccg cccgcagcaa tttcggcaac cgtgcaggta ccgccgcttt
     4321 tcagacggat cccctcatcg agacaggtgt cagggcgcga caggaagtcc cgcagaagtt
     4381 cgctggcttc ccgcgccacc acggcaaagc gtttgattcc ccgctcgacg gcgaattgcg
     4441 tgcattcgcg gccgatcttg ccgagaaact cg
//
LOCUS       DAOSDX010000110         4463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6870_length_4463_cov_2.708258, whole genome shotgun sequence.
ACCESSION   DAOSDX010000110 DAOSDX010000000
VERSION     DAOSDX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_4463_cov_2.708258"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLN02_03480"
     CDS             complement(<1..463)
                     /locus_tag="PLN02_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI51412.1"
                     /translation="MKSSSLARCLGALLLAAASTLAAAADHATPREARALFNEAVAYL
                     KANGPEKAWAAFSDPKGQFVRKDLYVYVIDRQGTYVANGAAPDSLIGLKVLDSVDAAG
                     LPLFREMIAVTEKQPEARIGYVWLNRKSNRVEPKFAWLHRQGDYILGVGYYA"
     gene            complement(497..1144)
                     /locus_tag="PLN02_03485"
     CDS             complement(497..1144)
                     /locus_tag="PLN02_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI51413.1"
                     /translation="MIRIILVDDHAVVRNGYRRLLDAEPGLQVVGEAASADEANALAL
                     RTVADVALVDLSLRGSSGIEAIRGMLARQPALKVLVLSMHEGAGHVTQALRSGAHGYL
                     TKYSEPAEVIDGIRRIAAGKRVFSPEIAQVLAAEAIDGDGVLAHLTPREFEVLRMLVH
                     GESASGIASSMHLSPKTVLNYLTLIRQKLDADNDFKLLHLAARHGLVDIHHALGA"
     gene            complement(1122..1544)
                     /locus_tag="PLN02_03490"
     CDS             complement(1122..1544)
                     /locus_tag="PLN02_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51414.1"
                     /translation="MKRRAGRLGRCARAFSGVLLLSLSCALPALAEAASLALYRVVQE
                     ALTNVVRHSGARHCRVGIECRAAALLLCIEDDGQGLPADRPAHQGGLLGMVERLDMVG
                     GRLQVAGGPGRGVQLQVSLPLQEEETKEEEQDDPHNSG"
     gene            complement(1541..2656)
                     /locus_tag="PLN02_03495"
     CDS             complement(1541..2656)
                     /locus_tag="PLN02_03495"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI51415.1"
                     /translation="MRILVLSVSAGAGHVRAAQSICAAGAALPGLELTHLDVLDLVSG
                     GFRKFYGSVYLKLVEKAPQLWAYLYDRTDHPPSSSWLESARRHVERLNTRRLDAEIAQ
                     VAPDAIVCTHFLPAELLSRRIASGQPTPPVWINVTDFDVHGLWLHPRIAGYCVATEEV
                     AVRLAARGIPEQQIHVTGIAVMPQFGKPPTRATAAAELGLDPARPTLLMMSGGAGVGG
                     IDRLAEQAALAFPEVQIVALAGRNEDLLRRLGEVAARHPGRVYPQGFTSTIERVMAAA
                     DLAITKPGGLTTSECLAMQLPMIVVSPIPGQEERNADTLLEAGCALKAVDTAALVYKI
                     RRLLDDPARLARMRESQVQLARPQAARAIVDLVRAAA"
     gene            complement(2750..3595)
                     /gene="rsmI"
                     /locus_tag="PLN02_03500"
     CDS             complement(2750..3595)
                     /gene="rsmI"
                     /locus_tag="PLN02_03500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOI51416.1"
                     /translation="MTNELIAALYVVPTPLGNLGDLTRRAEEVLGAVPWVACEDTRHS
                     APLLKQLGARARTLPAHQHNEHEAAARIVAKLQAGEAVALVSDAGTPGISDPGARIVA
                     AVRAAGCKVVPLPGACAATTALSAAGLADEHFLFYGFLPSKAGQRQQALAALAGHPYA
                     LVFYEAPHRVLETVADMAAVFGERTLVVARELTKLFENVDAMPLAAALDWFKADPNRQ
                     RGEFVLIVSGAPADAASGEGERVLRLLLDEGLGTKQAAKLAAAISGEAKNALYERALA
                     LKAAD"
     gene            3604..3966
                     /locus_tag="PLN02_03505"
     CDS             3604..3966
                     /locus_tag="PLN02_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOI51417.1"
                     /translation="MQAKTNDTTVARGREAESEAAAYLAGRGLRLRERNFRVRGGEID
                     LVMQDGPTVVFVEVRARSRSDFGDAGASIDAFKQERIVLAARHWLARHGECACRFDCV
                     LIDDGRLRWLRNAFAGEH"
     gene            4030..>4463
                     /locus_tag="PLN02_03510"
     CDS             4030..>4463
                     /locus_tag="PLN02_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOI51418.1"
                     /translation="MDLIARVAQHFEDSAQTKLNAVDALSAPIAAAIGTLTGALLEGG
                     KILACGNGGSAADAQHFAAELVGRFEAERQELAAIALSTDSSILTAVGNDYGFAHIFA
                     KQVRALGHAGDVLLAISTSGNSGNVIEAIKAAHEHDMRVIALT"
BASE COUNT          684 a         1521 c         1577 g          681 t
ORIGIN      
        1 cggcgtagta gccgacgccg aggatgtagt cgccctggcg gtgcagccag gcgaacttgg
       61 gttcgacgcg gttgctcttg cggttgagcc agacgtagcc gatgcgcgct tccggctgtt
      121 tctcggtgac cgcgatcatc tcgcggaaca gcggcaggcc ggcggcgtcg acgctgtcga
      181 ggaccttgag gccgatcagg ctgtccggcg cggcgccgtt ggcgacgtag gtgccctggc
      241 ggtcgatcac gtaaacatag aggtccttcc tgacgaactg gcctttcggg tcgctgaagg
      301 cggcccaggc tttttccgga ccgttggcct tgaggtaggc gacggcctcg ttgaacaggg
      361 cgcgggcttc gcgcggcgtc gcgtggtcgg cggctgcggc cagggtcgag gcggctgcga
      421 gcagcagggc gcccaggcag cgggcgagtg acgatgactt cattgtggtc tccgggtgtg
      481 gtggatggtt atggattcag gcgccgagcg cgtgatggat atccaccagg ccgtgccggg
      541 cggccaggtg gagcagtttg aaatcgttgt cggcgtcgag tttctggcgg atcagcgtga
      601 ggtagttgag aaccgtcttc gggctcaggt gcatcgagct ggcgatgccg ctggccgatt
      661 cgccatgcac cagcatgcgc aggacctcga attcgcgcgg cgtcaggtgg gccagcacgc
      721 cgtcgccgtc gattgcctcg gcggccagga cctgggcgat ttccggggaa aagacgcgct
      781 tgccggcggc gatgcggcgg atgccgtcga tcacctcggc cggttcgctg tacttggtca
      841 ggtagccgtg ggcgccgctg cgcagcgcct gggtgacgtg gccggcgcct tcgtgcatgg
      901 acagtaccag caccttgagc gccggctggc gggcgagcat gccgcggatc gcctcgatgc
      961 cgctgctgcc acgcaggctg aggtcgacca gggcaacgtc ggcgacggtg cgcagggcca
     1021 gcgcgttcgc ctcgtcggcg ctcgccgcct cgccgaccac ctgcagcccg ggctcggcgt
     1081 cgagcaggcg gcggtagccg ttcctgacca cggcgtggtc gtcaaccaga attatgcgga
     1141 tcatcttgtt cttcctcctt tgtctcttcc tcttgcagcg gcaggctgac ctgcaactgc
     1201 accccgcgcc ccggcccgcc cgccacctgc aggcggccgc cgaccatgtc caggcgctcg
     1261 accatgccga gcaggccgcc ctgatgcgcc gggcggtcgg ccggcagccc ctggccgtcg
     1321 tcctcgatgc acagcagcaa cgccgctgcc cggcactcga tgccgacccg gcagtggcgg
     1381 gcgccgctgt ggcgcacgac attggtcagc gcctcctgca ccacccggta cagcgccagg
     1441 ctagccgcct cggccagcgc cggcagggcg cacgacaacg acaggaggag cacgccggaa
     1501 aacgcccggg cgcaccgccc gagccgcccc gcccggcgct tcacgctgcc gcccgcacca
     1561 ggtcgacaat ggcgcgcgcc gcctgcggac gggccagctg gacctggctt tcgcgcatgc
     1621 gggcaaggcg cgccggatcg tcgagcaggc ggcggatctt gtagaccagc gccgcggtgt
     1681 cgaccgcctt aagggcacaa ccggcttcca gcaaggtgtc ggcgttgcgt tcctcctggc
     1741 cgggaatcgg cgagacgacg atcatcggca actgcatggc caggcattcc gaagtggtca
     1801 ggccgcccgg cttggtgatc gccagatcgg ccgctgccat gacgcgctcg atggtcgagg
     1861 tgaagccctg cgggtacacg cgtcccggat gacgggcggc gacttcgccg aggcgacgca
     1921 gcaagtcctc gttgcgcccg gccagggcga cgatctgcac ctcagggaaa gccagtgccg
     1981 cctgctcggc caggcggtcg atgccgccga cgccggcgcc gccggacatc atcagcaggg
     2041 tcgggcgtgc cgggtcgagg ccgagttcgg cggcggccgt cgcccgcgtc ggcggcttgc
     2101 cgaactgcgg catgacggcg atcccggtga cgtggatttg ctgctccggg atgccgcggg
     2161 cggccaggcg cacggcaact tcctcggtgg cgacgcaata gccggcgatg cgcgggtgca
     2221 gccacaggcc atggacgtcg aaatcggtga cgttgatcca caccggcggc gtcggctggc
     2281 cgctggcgat ccgccgcgac agcagctcag ccggcaggaa atgcgtgcac acgatggcgt
     2341 cgggggcaac ctgggcgatt tccgcgtcga ggcggcgggt attcaggcgc tcgacatgac
     2401 gccgcgccga ttccagccag gaactgctcg gcggatgatc ggtgcgatcg taaagatagg
     2461 cccacagttg cggcgccttc tcgaccagct tgaggtagac gctgccgtag aacttgcgga
     2521 agcccccgga aaccagatcg agcacgtcga gatgggtcag ttccaggccc ggcagcgccg
     2581 cacctgctgc acaaatcgat tgcgcggcgc ggacatggcc ggcgccggcg gacacactga
     2641 gaacgaggat gcgcatgcgg ttctggccgg gacagccggc gacggtcggg aaagcggccg
     2701 attctggcag tcaccgccgc accctgtcaa tgcattcggg ataacacctt cagtccgccg
     2761 ccttcagcgc cagcgcgcgc tcgtagaggg cgttcttcgc ctcgccgctg atcgccgccg
     2821 ccagcttcgc cgcctgtttg gtgccgagtc cttcgtcgag cagcaggcgg agtacgcgct
     2881 cgccttcgcc gctggcggcg tcggccggcg cgccggagac gatcagcacg aattcgccgc
     2941 gctgccggtt gggatcggcc ttgaaccagt cgagggcggc ggcgagcggc atggcgtcga
     3001 cgttctcgaa caacttggtc agttcgcggg cgacgaccag cgtgcgttcg ccgaacaccg
     3061 cggccatatc ggcgacggtc tcaagaacgc ggtgcggcgc ctcgtagaag accagcgcgt
     3121 agggatggcc ggcgagtgcc gcgagcgcct gctgacgctg gccggccttg ctaggcaaaa
     3181 agccgtagaa caggaaatgc tcgtcggcca gcccggcggc ggacagcgcg gtggtcgccg
     3241 cgcaggcgcc gggcagcggc acgaccttgc agccggcggc gcgaacggcg gcgacgatgc
     3301 gcgcgccggg gtcggaaatg cccggcgttc cggcatccga gaccaacgcg acggcctcgc
     3361 cggcctgtaa tttggcgacg atgcgggcag cggcttcatg ttcgttgtgc tggtgcgccg
     3421 gcagcgtccg ggcgcgggcg ccgagctgct tcaacagcgg cgcgctgtgc cgggtgtcct
     3481 cgcaggcgac ccagggcacg gcgcccagga cttcctcggc ccgccgcgtc aggtcgccga
     3541 ggttccccag cggcgtcggc acgacgtaca atgccgcgat caattcgttt gtcattttcg
     3601 gcgatgcagg caaaaaccaa cgataccacc gtcgcccgcg gccgcgaagc cgagagcgaa
     3661 gcagcagcct acctggcggg gcgcggcctg cgcctgcgcg agcgcaactt ccgggtgcgc
     3721 ggcggcgaga tcgaccttgt catgcaggac gggccgaccg tcgtcttcgt cgaggtgcgc
     3781 gcccgcagcc gcagcgattt cggcgacgcc ggcgccagca tcgacgcctt caagcaggag
     3841 cgcatcgtcc tcgccgcccg ccactggctg gcccgccacg gcgaatgcgc ctgccgcttc
     3901 gactgcgtgc tgatcgacga cggccgcctg cgctggctgc gcaacgcctt tgccggcgag
     3961 cattgaagcg acgccgccac ggcacgctga ggtaaaatca tgagccgatc atctgaaaga
     4021 agccgttcca tggatttgat tgcccgcgtc gcccagcatt tcgaggacag cgcccagacc
     4081 aagctcaacg ccgtagacgc cctctcggcg ccgatcgccg ccgccatcgg cacgctgacc
     4141 ggcgccctgc tcgaaggcgg caagatcctc gcctgcggca atggcggttc ggccgccgac
     4201 gcccagcatt tcgccgccga actggtcggc cgcttcgagg ccgagcgcca ggagctggcg
     4261 gcgatcgccc tgtccacaga cagctcgatc ctgaccgcgg tcggcaacga ctacggtttc
     4321 gcccacatct tcgccaagca ggtgcgcgcc ctcggccacg ccggcgacgt gctgctggca
     4381 atctcgacct cgggcaattc cggcaacgtc atcgaagcga tcaaggccgc ccacgaacac
     4441 gacatgcgcg tcatcgcgct gac
//
LOCUS       DAOSDX010000111         4462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6873_length_4462_cov_3.405945, whole genome shotgun sequence.
ACCESSION   DAOSDX010000111 DAOSDX010000000
VERSION     DAOSDX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6873_length_4462_cov_3.405945"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLN02_03515"
     CDS             <1..564
                     /locus_tag="PLN02_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HOI51419.1"
                     /translation="GATTRAGMLTNPLRDRFGIVARLEFYNAEELRSIVSRSASLLNA
                     PIDPDGALEIAKRSRGTPRIANRLLRRVRDYAEVKGDGSIGRDIADRALHMLDVDPAG
                     LDLMDRKLLSAVIDKFGGGPVGVDNLAAAIGEARDTIEDVLEPYLIQQGYLQRTLRGR
                     IATPAIYRHLGLQEPAGAAVRDLLSDS"
     gene            complement(565..2025)
                     /locus_tag="PLN02_03520"
     CDS             complement(565..2025)
                     /locus_tag="PLN02_03520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI51420.1"
                     /translation="MPGKLRLIDRRSLILLLGLLLSVGFFATTLFGYFVSREAIRSAI
                     IGQDLPLTASNIYSEIQKDLVRPVLISSTMAHDTFLRDWVLGGERDVRAMARYLQEVK
                     VRHKAFSSFFVSEKSTNYYTGDGILKQVSPSEPRDVWYYRVRKMSEDYEINVDPDLAN
                     RDALTIFINYRVFDFDNRYIGATGIGLTVDSVRRLIREYQARFKRTIYFTDASGRVVL
                     QGDQAQLPNLRQRPGLAAQLDTILKQGGGMYQFVDEGDNHILNVNYLPELQWYLFVEQ
                     NEDVALAGVRQTLYINLAISLLVTLIVTWLCYITLGHYQSRIEKMASTDKLTGLLNRH
                     AFGILIDKLIASYRRSPRPISFLLADIDHFKAVNDGYGHRVGDRVLVQVAAALRAQLR
                     EADFAVRWGGEEFLLVLPGCTADEARSVAEKLRQAVAASIPCAEWQELRVTVSIGVSE
                     FDGEETPDQAIGRADNALYKVKQGGRNGVAMAAASA"
     gene            2150..2572
                     /gene="ybgC"
                     /locus_tag="PLN02_03525"
     CDS             2150..2572
                     /gene="ybgC"
                     /locus_tag="PLN02_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HOI51421.1"
                     /translation="MSAHAHQPSFSIPVRVYYEDTDAGGVVYYANYLRFFERCRTEWM
                     RSAGYGQAQLAAEAGIGFVARKASCEYLKPARLDDLVEVGLSVEKLTRVRVVFRQSVR
                     RGDEELVTGEVEIACVNMATMTPAPIPDFLHSRLEQLK"
     gene            2569..3246
                     /gene="tolQ"
                     /locus_tag="PLN02_03530"
     CDS             2569..3246
                     /gene="tolQ"
                     /locus_tag="PLN02_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOI51422.1"
                     /translation="MNVTQDLSILHLILEASAVVQIVMALLAGVSFMSWYYIAMKWFA
                     VRQARAKTEDFERDFWSGGDLSNLYQSAVEGRHQTGSMERIFEAGFREFTKLRGQKHL
                     DSKDVVDGSRRAMRATYQREMDALEAHLAFLASVGSVSPYIGLFGTVWGIMHAFRGLS
                     NVGQATLASVAPGIAEALVATAIGLFAAIPAVLAYNRFSHDIDRLAVRYESFMEEFSN
                     ILQRQMR"
     gene            3250..3648
                     /locus_tag="PLN02_03535"
     CDS             3250..3648
                     /locus_tag="PLN02_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HOI51423.1"
                     /translation="MRQRRLKNEINVVPYIDVMLVLLVIFMVATPMMTTGSVELPSAG
                     TAPQKPDKYLKVQVDKDGALVLFDLDGKERPLAGMRELRDQLAGRQEARETAVLVAGD
                     GEAPYKEVIRVLDEVKKAGFSRVALETAVK"
     gene            3653..4438
                     /locus_tag="PLN02_03540"
     CDS             3653..4438
                     /locus_tag="PLN02_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOI51424.1"
                     /translation="MRPETPVEPGKKKALTFTLLVHLALVGALFLGVQWKRSEPEAMQ
                     VELWSPTPQKATRVAPPEPAPEPKPEPKPEPKPEPKPEPPKKLETPKPPAIVVKEEKK
                     KPEPKKPEPKPEPPKPEPKPEPKPVPKPKNDPFKEMLERENHQRQAVAEQDRLAELAN
                     QAQKAAANRRGLESYAAKIRAKVRGNIVLPPNLPGNPEALFEVNQLPGGDVLSIRLKR
                     SSGVPALDEAIERAIRKSSPLPKPDDPSQFQRTLEIKYKPFEE"
BASE COUNT          854 a         1488 c         1415 g          705 t
ORIGIN      
        1 ggcgcgacca cccgcgccgg catgctgacc aacccgctgc gcgaccgctt cggcatcgtc
       61 gcccgcctcg agttctacaa cgccgaggag ttgcgcagta tcgtcagccg ctcggcctcg
      121 ctgctgaacg cgccgatcga ccccgacggc gcgctggaaa tcgccaagcg ctcgcgcggc
      181 acgccgcgca tcgccaaccg cctgctgcgc cgggtgcgcg attacgcgga agtaaagggc
      241 gacggcagca tcggccgcga catcgccgac cgcgccctgc acatgctcga cgtggacccg
      301 gccggcctcg acctgatgga caggaagctg ctgtcggcgg tgatcgacaa gttcggcggc
      361 ggcccggtcg gcgtcgacaa cctggcggcg gcgatcggcg aggcgcgcga caccatcgag
      421 gatgtgctcg agccctacct gatccagcaa ggctacctgc agcgcacgct gcgcgggcgc
      481 attgcgacgc cggcgatcta ccgccacctc ggcctgcagg aaccggccgg cgcagcggtg
      541 cgcgacctgc tttcggacag ttgatcaggc gctggcggcg gccatcgcca cgccgttgcg
      601 gccgccctgc ttgaccttgt agagcgcgtt gtcggcgcgg ccgatcgcct ggtccggggt
      661 ttcctcgccg tcgaactcgc tgacgccgat gctgaccgtc acccgcagtt cctgccattc
      721 ggcgcaggga atcgacgcgg cgacggcctg gcgcagtttt tcggcgacgc tgcgcgcctc
      781 gtcggcggtg caaccaggca ggaccaacag gaattcctcg ccgccccagc gcaccgcaaa
      841 atcggcctcg cgcaactggg cacgcaaggc ggcggcaacc tggaccagga cgcgatcgcc
      901 gacgcgatgc ccgtacccgt cgttgaccgc cttgaaatgg tcgatgtcgg cgagaaggaa
      961 gctgatcggt cgcgggctgc gccggtagct ggcgatcagc ttgtcgatca ggatgccgaa
     1021 ggcgtgccgg ttgagcagcc cggtcagctt gtcggtcgac gccatcttct cgatccgcga
     1081 ctggtaatgg ccgagcgtga tgtagcagag ccaggtaacg atcagcgtca ccagcaggct
     1141 gatcgccaga ttgatgtaca aggtctggcg aacgccggcg agcgcgacgt cctcgttctg
     1201 ctcgacgaac aggtaccact gcagttcggg caggtaattg acgttgagga tgtggttgtc
     1261 gccctcatcg acgaactggt acatgccacc gccctgcttg aggatggtgt cgagctgcgc
     1321 cgccagcccg gggcgctggc gcaggttggg tagctgcgcc tggtcgccct ggaggacgac
     1381 gcgaccgctg gcgtcggtga agtagatggt gcgcttgaag cgcgcctggt attcgcggat
     1441 caaccggcgc accgaatcaa cggtgaggcc gatgccggtg gcgccgatgt agcggttgtc
     1501 gaagtcgaac acccggtaat tgatgaagat ggtcagcgcg tcgcggttgg ccaggtcggg
     1561 atcgacgttg atctcgtaat cctcgctcat cttgcgcacc cggtaatacc agacgtcgcg
     1621 cggttcgctc ggcgaaacct gcttcaggat gccgtcaccg gtgtagtagt tggtgctctt
     1681 ctcggaaacg aagaagctgc tgaaggcctt gtgcctgacc ttgacctcct gcaggtagcg
     1741 cgccatcgcc cgcacgtcgc gttcgccgcc gagcacccag tcgcgcagga aggtgtcgtg
     1801 cgccatggtc gacgagatca gcaccggccg caccagatcc ttctggattt ccgagtagat
     1861 gttgctcgcc gtcagcggca gatcctggcc gatgatcgcg ctgcggatcg cctcgcgcga
     1921 gacgaagtag ccgaacagcg tcgtggcgaa gaagccgacg ctgagcagca ggccgagcag
     1981 caggatcagg ctgcgacgat cgatgaggcg gagttttccg ggcatggtgg gcattatgcg
     2041 caagggaccg ccagaagacc agcttccccg gcgacagttc aaaatggcga tattttcgtg
     2101 gaaacaagcc gttacaatag gatgttccag cacaagataa aatcgccgga tgtcagccca
     2161 cgcccaccag ccctcgtttt ccatcccggt ccgcgtttac tacgaggaca cggatgccgg
     2221 cggcgtcgtc tattacgcca attacctgcg tttcttcgag cgctgccgca ccgaatggat
     2281 gcgcagcgcc ggctacggcc aggcccagct cgccgccgaa gccggcatcg gcttcgtcgc
     2341 ccgcaaggcc agttgcgaat acctgaagcc ggcgcggctc gatgatctgg tcgaggttgg
     2401 cctgagcgtc gagaaactca cccgcgtccg cgtcgtcttc cgccagtcgg tacgccgcgg
     2461 cgacgaggaa ctggtcaccg gcgaggttga aatcgcctgc gtcaacatgg cgacgatgac
     2521 ccccgccccg attcccgatt tcctgcattc ccggctcgaa caactcaaat gaacgtcacc
     2581 caggaccttt ccatcctcca tctgatcctc gaggcttcgg ccgtcgtcca gatcgtcatg
     2641 gcgctgctcg ccggcgtctc cttcatgtcc tggtactaca tcgcgatgaa gtggttcgcg
     2701 gtacgccagg cgcgcgccaa gaccgaggac ttcgagcgcg acttctggtc cggcggcgat
     2761 ctcagcaacc tctaccagag cgcggtcgaa ggccgccacc agaccggctc gatggagcgt
     2821 atcttcgagg ccggcttccg tgaattcacc aaactgcgcg gccagaaaca cctcgactcg
     2881 aaggacgtgg tcgatggttc gcggcgcgcc atgcgcgcca cctaccagcg cgagatggac
     2941 gccctcgaag cgcacctcgc cttcctcgcc tcggtcggct cggtatcgcc ctacatcggc
     3001 ctgttcggta ccgtctgggg gatcatgcac gccttccgcg gcctctccaa cgtcggccag
     3061 gcgacgctcg cctcggtcgc ccccggcatt gccgaagccc tggtggcgac ggcgatcggc
     3121 ctgttcgcgg cgatcccggc agtgctcgcc tacaaccgct tctcgcacga catcgaccgc
     3181 ctggcggtgc gctacgagtc gttcatggaa gagttctcca acatcctgca gcggcagatg
     3241 cgttaagcca tgcgccagcg ccgactcaag aacgaaatca acgtcgtccc ctacatcgac
     3301 gtgatgctgg tgctgctcgt catcttcatg gtggcgacgc cgatgatgac gaccggctcg
     3361 gtcgagttgc cgagcgccgg cacggcacca caaaaaccgg acaagtacct gaaggtgcag
     3421 gtcgacaagg acggcgccct ggtcctcttc gatctcgacg gcaaggaacg cccgctggcc
     3481 ggcatgcgcg agttgcgcga ccagctcgcc ggtcgccagg aagcccgcga gacggcggtg
     3541 ctggtcgctg gcgacggcga agcgccgtac aaggaagtga tccgcgtgct ggacgaggtc
     3601 aagaaggccg gcttctcccg cgtcgcgctg gaaaccgcgg tcaagtaagg cgatgcgtcc
     3661 ggaaaccccc gtcgaaccag gcaagaagaa ggcactgacc ttcacgctgc tggtccatct
     3721 ggcactggtc ggcgcccttt tcctcggcgt gcagtggaag cgcagcgagc cggaggcgat
     3781 gcaggtcgaa ctgtggtcgc cgacaccgca gaaggcgacg cgcgtcgccc cgccggaacc
     3841 ggcaccggag ccgaagcccg aacccaagcc ggagccaaag ccggaaccga agcccgagcc
     3901 gccgaaaaag ctggaaacgc cgaagccgcc agccatcgtc gtcaaggaag aaaagaagaa
     3961 gccggaaccg aaaaagccgg agcccaagcc cgaaccgccc aaaccggaac cgaaacccga
     4021 acccaagccg gtaccgaagc cgaagaacga cccgttcaag gaaatgctcg aacgcgaaaa
     4081 ccaccagcgc caggctgtcg ccgagcagga ccgcctggcc gaactggcca accaggcgca
     4141 gaaggcggcc gccaatcggc gcggcctgga aagctacgcg gcgaagatcc gcgccaaggt
     4201 gcgcggcaac atcgtgctgc cgcccaacct gcccggcaat ccggaagcgc tgttcgaggt
     4261 caaccagttg ccgggcggcg acgttctgtc gatccgcctg aaacgatcaa gcggcgtgcc
     4321 ggcgctcgac gaagccatcg aacgggcgat ccgcaaatcc tcgccgctgc cgaaacccga
     4381 cgaccccagc caattccagc gcacgctcga aatcaaatac aagccatttg aagagtaaaa
     4441 tcgcgcacaa ttcagagaaa cc
//
LOCUS       DAOSDX010000112         4460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6875_length_4460_cov_3.600681, whole genome shotgun sequence.
ACCESSION   DAOSDX010000112 DAOSDX010000000
VERSION     DAOSDX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6875_length_4460_cov_3.600681"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLN02_03545"
     CDS             complement(<1..715)
                     /locus_tag="PLN02_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI51425.1"
                     /translation="MSHSLAISRRQSALLLLPAAIFFTAFFLLPMLRLFFIGASGKAG
                     WSAYLAIVTDANYFRALWTTLAVAAVTTLLTLAISGISGVFLQRHAFPGRALLVSALT
                     LPLAFPGVVVGFMVIMLAGRQGLIGEVFAMLGGERPVFAYSLAGLCMGYVYFSIPRTI
                     LTIMAAAEKLDPRLEEAARSLGGSPWHVVRDVILPGLRPAFVSAGAICFATAVGAFGT
                     AFTLATRIEVLPMVIYTEFT"
     gene            complement(777..1799)
                     /locus_tag="PLN02_03550"
     CDS             complement(777..1799)
                     /locus_tag="PLN02_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOI51426.1"
                     /translation="MIPTRFARTLAAVVLGGAALFGAAAVSAQETAVCYNCPPEWADW
                     GSQLKAIHKRLNIVVPPDNKNSGQAIAALIAEKANPVADVTYLGGIAADPARDAGVLT
                     PYKPKGWDKIPASLKDPEGHWFTIHSGTLGLFVNKAALGGKPVPQSWSDLLKPEYKGL
                     VGYLDPTSAAVGQLGVMAINLALGGSYDNLDPAIRFFKELQKNQPIVPKQTSYARVIS
                     GEIPILLDYDFNAYRGQYKDMAPVAFVIPKEGTLVFPYVMGLVKNGPHAVNGRKMLDF
                     VLSDESQAMWGDAYLRPVFADKLSAAAKARFLPEREYARAKPVDLKKLAAAQAAIIER
                     YRNEVK"
     gene            complement(1796..2605)
                     /locus_tag="PLN02_03555"
     CDS             complement(1796..2605)
                     /locus_tag="PLN02_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOI51427.1"
                     /translation="MQAEQRARAIVEVVRERGFQTIESLAGEFAVTPQTIRRDVNALS
                     DQGMLRRRHGGVELPQQGENLAYPARQQLNLAAKRAIAQLVARDVPARASLFFGIGTT
                     PEQCALALVHHPQLRVMTNNLNVAMRLAHNPDCELIVAGGRVRNLDLDVVAGEAHDFF
                     ARFNVDIGIFGVGAVAPDGTLLDFSLDEVAMRESLMRHCRQRFLVLDHSKFARDATVR
                     GGHITDATVVYSDRPLPSPIRAMLVAAGVRAVVPAEAAKVSQVSLTPGECS"
     gene            2863..>4460
                     /locus_tag="PLN02_03560"
     CDS             2863..>4460
                     /locus_tag="PLN02_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51428.1"
                     /translation="MKLRHWLLIVAGIPGGLFVPLSYALFAGLALLLGYIACHPPRWV
                     ELQRACAYLFGGLCTGAGLQVLAGYGMRGHLLSMIIAPLVLWLVTAVIFSLLRDRYSL
                     TPAPALASPPLAGAGRGSSAWSGTASGGSTQAGSDGCRVLWRFYDTGEICMGGPTYGD
                     VVFSNRCAFRDVGPSVAIADDGRYAALTQPSRGTWGMLLVDFERRLTYYLSAREVPWE
                     IDAISDGKIHGRVSPLTGDTPVSLDIDHLIAEQPAIPLVEDDGWWLLDDAQREPLPRH
                     DAVSVGSSGGRHTLIFVPDRRPLRDNPFLRFAAPSYALLVDGYLLDIETRHTRACWID
                     STEHEGRFLVIGERLFDLMPDGVFAPAAARELPLTSVDEFPHASLGEMRAGDPGMLLA
                     EVALLQRSVAHDRAESCQESSTYPWDEEEVDYRDADGQPQRRTRTRIERHALYRIDLA
                     ACFNGGDQRLATVIELKNRAHPDYRAELFPLPGYGPATPYAPYRCATSCGVDPGPVLG
                     EFIWSHCGRYLALVIAEPPPAVPGT"
BASE COUNT          769 a         1493 c         1476 g          722 t
ORIGIN      
        1 tggtgaattc ggtatagatg accatcggca gcacctcgat ccgcgtcgcc agcgtgaacg
       61 ccgtgccgaa ggcgccgacg gcggtggcga agcagatggc gccggccgag acgaaagccg
      121 gccgcagccc cggcaggatc acgtcgcgca cgacatgcca gggcgaaccg ccgagcgaac
      181 gcgctgcctc ttcgaggcgc ggatcgagtt tttcggcggc ggccatgatg gtcaggatgg
      241 tgcgcggaat cgaaaaatag acataaccca tgcacaggcc ggccagcgaa taggcgaaca
      301 ccgggcgctc gccgccgagc atggcgaaaa cctcgccgat cagcccctgg cgaccggcca
      361 gcatgatcac catgaaaccg acgacgacgc cgggaaaggc cagcggcaag gtcagcgccg
      421 agaccagcag ggcgcgcccg ggaaaggcat ggcgttgcag gaagacgccg gagatgccgc
      481 tgatcgccag cgtcagcagg gtggtcacgg cggccacggc aagcgtcgtc cacagggcgc
      541 ggaagtagtt cgcgtcggtg acgatcgcca ggtaggcgct ccagccggct ttgccgctgg
      601 caccgatgaa gaacaggcgc agcatcggca acaggaagaa ggcggtgaag aagatggccg
      661 ccgggagcag cagcaaggcg ctctggcgac gggaaatggc gagggaatgg gacatgggca
      721 aggggctggt gcacggggac tggcacgcgg cgccggagcg ccgcgtgccg cgtcgattac
      781 ttcacttcgt tgcgataacg ttcgatgatc gccgcctggg cagccgccag tttcttcaga
      841 tcgaccggct tggcgcgggc gtactcgcgc tcgggcagga acctggcctt ggctgcggcc
      901 gacagcttgt cggcgaaaac cgggcgcagg taggcatcgc cccacatcgc ctggctttcg
      961 tcggagagga cgaaatcgag cattttgcga ccgttgaccg catgcggccc gttcttgacc
     1021 agtcccatga catacgggaa gaccagcgtg ccttccttgg gaatgacgaa ggcgaccggc
     1081 gccatatcct tgtactgacc gcgataggcg ttgaaatcgt agtcgagcag gatgggaatc
     1141 tcgccggaga tgacgcgggc gtaggacgtc tgcttcggca cgatcggctg gttcttctgc
     1201 agttccttga agaagcggat cgccgggtcg aggttgtcgt agctgccgcc gagcgccagg
     1261 ttgatggcca tgacgccgag ctggccgacc gccgccgagg tcgggtcgag atagccgacc
     1321 aggcccttgt attccggctt gagcaggtcg ctccagctct gcggtaccgg cttgccgccg
     1381 agcgccgcct tgttgacgaa gagaccgagg gtgccggaat ggatggtgaa ccagtggcct
     1441 tccgggtcct tcagactggc cggaatcttg tcccagccct tgggcttgta cggggtcagc
     1501 acgccggcat cgcgcgccgg gtcggcggcg atgccgccaa ggtaggtgac gtccgccacc
     1561 gggttggcct tctcggcgat cagcgcggcg atcgcctggc cggagttctt gttgtccggc
     1621 ggcacaacga tgttcagacg cttgtggatc gccttcaact gcgaccccca gtccgcccat
     1681 tccggcggac agttgtagca gacggcggtt tcctgcgcgc tgacggcagc ggcgccaaac
     1741 agggcggcac cgccgaggac gacggcggcc agggtacggg caaaacgggt cgggatcatg
     1801 agcattctcc aggggtgagg gaaacttgcg aaaccttggc ggcttccgcc ggcaccaccg
     1861 cacgcacacc ggcggcgacg agcatggcgc ggatcggcga cggcagcgga cgatcgctat
     1921 agaccaccgt cgcatcggtg atgtgaccgc cacgcacggt ggcatcacgg gcaaacttgc
     1981 tgtggtcgag gaccaggaaa cgctggcggc aatggcgcat cagcgattcg cgcatggcca
     2041 cctcgtcgag gctgaaatcg agcagcgtgc catccggcgc cacggcgccg acgccaaaaa
     2101 tgccgatatc gacattgaag cgggcaaaaa agtcatgcgc ctcgccagcc accacgtcga
     2161 ggtccaggtt gcgcacccgg ccaccggcaa cgatcagttc gcagtccggg ttatgcgcca
     2221 gccgcatggc gacattcagg ttgttggtca tgacccgtaa ctgcgggtga tgcaccaggg
     2281 ccagcgcgca ctgttcgggc gtcgtaccga tgccgaagaa cagcgaggca cgcgccggaa
     2341 catcgcgcgc cacgagttgg gcaatcgccc gcttggcagc cagattgagt tgctgacgcg
     2401 ccggataggc caggttttct ccctgctgcg gcagttcgac accaccgtga cggcggcgca
     2461 gcatgccttg atcgctcagc gcattgacgt cgcgtcggat cgtctgcggc gtcacggcaa
     2521 actcgccggc cagcgactcg atggtctgga aacctcgctc gcgtaccacc tcgacgatcg
     2581 cccgggcgcg ttgttccgcc tgcatgcctg tcctttcctg aatgggatgt ctcgattccg
     2641 cgcctcggcg cgttcgttcg aaatgctagg cggggcatat ttcaggaaga tggcgctcaa
     2701 atgaaacctg aaacactcat tcgaaagcgc ggaacgaaaa gggcaattcc ccgatgagca
     2761 tgcttttggc ggagcgatcg cctatcatcc cgttttcatg aaaagtcgcc cttctgccat
     2821 gccatcccgg ccttccccct gcgtcgctct gccgaacgcg cgatgaaact ccgtcactgg
     2881 ctgctgatcg tcgccggcat tccgggcggc ctgttcgtcc cgttgagcta cgcgctgttc
     2941 gccggattgg cgctgctcct cggctacatc gcctgccacc cgccgcgctg ggtcgaactg
     3001 cagcgcgctt gcgcctacct gttcggcggg ctgtgcaccg gcgccgggct acaggtgctg
     3061 gccggctacg gcatgcgcgg gcacctgctg tcgatgatca tcgccccgct cgtcctctgg
     3121 ctggtgacgg cggtgatctt cagcctgctg cgggatcgct attcgctgac cccggccccc
     3181 gctttggcgt ctcccccgct ggccggcgcc gggcgtggca gttcggcctg gtccggcacg
     3241 gccagcggcg gctcgacgca agccggcagc gacggctgcc gcgttctctg gcgcttttac
     3301 gataccggcg aaatctgcat gggcgggccg acctacggcg acgtggtgtt cagcaaccgc
     3361 tgcgccttcc gggacgtcgg accgtcggtg gcgatcgccg acgacgggcg ttacgcggcg
     3421 ctgacgcagc cgtcgcgcgg cacctgggga atgctgctgg tcgacttcga acgccgcctc
     3481 acctactacc tgtcggcacg cgaagtgccg tgggagatcg acgccatcag cgacggcaag
     3541 attcacggcc gggtttcgcc gctcaccggc gatacgccgg tcagcctcga catcgaccac
     3601 ctgatcgccg aacagccggc catcccgctg gtcgaagacg acggctggtg gctgctcgac
     3661 gacgcgcaac gcgagccgct gccgcgccac gacgcggtca gcgtcggcag cagcggcggt
     3721 cgccacacgc tgatcttcgt tcccgaccgc cgaccgctac gcgacaaccc cttcctgcgc
     3781 ttcgcggcgc cgagctatgc gctactcgtc gacggctatc tgctcgatat cgaaacgcgc
     3841 cacaccaggg cctgctggat cgacagcacc gaacacgaag gacgctttct ggtcatcggc
     3901 gagcgcctct tcgacctgat gccggacggc gtcttcgccc ccgccgccgc ccgcgaactg
     3961 ccgctcacca gcgtcgacga atttccgcac gccagcctgg gcgagatgcg cgccggcgac
     4021 cccggcatgc tgctcgccga agtcgccctg ttgcagcgct cggtcgccca cgaccgcgcc
     4081 gaaagctgcc aggaatcgtc gacctatccc tgggacgaag aagaggtcga ctaccgggat
     4141 gccgacggcc agccgcagcg ccgcacgcgt acccggatcg agcgccacgc gctctaccgc
     4201 atcgacctgg cggcctgctt caacggtggc gaccagcgcc tggcgacggt gatcgaactg
     4261 aagaaccgcg cccatcccga ctaccgcgcc gaacttttcc ccctgcccgg ctacggcccg
     4321 gccacgccct acgcccccta ccgctgcgcg acttcctgcg gcgtcgatcc cggcccggtg
     4381 ctcggcgaat tcatctggtc gcactgcggc cgctacctgg cgctggttat cgccgagccg
     4441 ccgccggcgg taccggggac
//
LOCUS       DAOSDX010000113         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6918_length_4437_cov_3.369238, whole genome shotgun sequence.
ACCESSION   DAOSDX010000113 DAOSDX010000000
VERSION     DAOSDX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6918_length_4437_cov_3.369238"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="pstB"
                     /locus_tag="PLN02_03565"
     CDS             <1..748
                     /gene="pstB"
                     /locus_tag="PLN02_03565"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOI51429.1"
                     /translation="EISIRNLNFYYGKAIALKNINLEIYKGKVTAFIGPSGCGKSTLL
                     RTINRMYDLYPGQHADGELIVAGKNILEKGVDTIELRAKVGMVFQKPTPFPMSIYENI
                     AFGVRMHEKLTRNEMDDRIEWALTKAALWNEVKDKLNKPGTGLSGGQQQRLCIARGVA
                     VKPQVLLLDEPTSALDPISTMRIEELIHELKREFTIAIVTHNMQQAARVSDYTAYMYL
                     GELVEFGKTDELFIKPQDKRTEDYITGRMG"
     gene            762..1451
                     /gene="phoU"
                     /locus_tag="PLN02_03570"
     CDS             762..1451
                     /gene="phoU"
                     /locus_tag="PLN02_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOI51430.1"
                     /translation="MNETLHVSSQFDEDLARLRTHVLQMGGVVETQISAAIEAYSSGE
                     VASVKTIVETDRRINQLERSIDEDCAHIIAQRQPAASDLRLVLGISKIVTDLERTGDE
                     AKKIAKSVRRIYEAGHQPTQYGIGIRHLAEAVLALVRRSLDAFARQDAAQAIEVIRAD
                     QDVDLEFKSIIRQLITHMMEDPRTITTSLEIMAISRAIERIGDHAKNICEQVIYVVEG
                     RDIRHDKEAIK"
     gene            1448..2134
                     /gene="phoB"
                     /locus_tag="PLN02_03575"
     CDS             1448..2134
                     /gene="phoB"
                     /locus_tag="PLN02_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HOI51431.1"
                     /translation="MTPTILVVEDEPAIQELVVINLKHAGFLVVRAGSAEEADSAIRA
                     ALPDLIVLDWMLPGQSGVALARKLRADERTREIPIIMLTARVHEEDKVQGLDAGADDY
                     VTKPFSPKELVARVRAVLRRRAPHLAGEAVEVGSLQINPATHRVVAAGQSVDLGPTEF
                     RLLFFFMTHAERVYTRAQLLDEVWGDHVFIEERTVDVHIRRLRAALEPSGHHERVETV
                     RGTGYRFRGA"
     gene            2140..3420
                     /gene="phoR"
                     /locus_tag="PLN02_03580"
     CDS             2140..3420
                     /gene="phoR"
                     /locus_tag="PLN02_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HOI51432.1"
                     /translation="MQRALFYGVLTAAVAFPLGYFVEAWMGWVLFYAGLSLHLAMHYR
                     NFSRLERWTRKPVLDASLEGDGDWDRIFRRLYRHEKELLEKIELRERDIARLIAAVHA
                     MNDGIVLLDGNFYIQFCNKTAERQLGLDSARDRGASIANILRQPEFVAYLAEADFSRP
                     LTLRLDRYFERVLSLYLILYAEDCWLLQVKDITQTDRLDSMRRDFVANVSHELRTPLT
                     VLSGFVETLQEIDVDAESRKHYLQLMGQQSTRMLSIVQDLLTLSSIESAPPPQDEVVD
                     MATLVDKLHRDAEALSAGRHTIHLETEGQGDLLGAESELISAFANLVANAVRYTPNGG
                     TITLSWKVSPAGAEFAVQDTGIGIDAKHIPRLTERFYRVDRGRSRDAGGTGLGLAIVK
                     HSLSRHQATLEVTSKPGEGSRFAAKFPPSRVSGV"
     gene            complement(3421..>4437)
                     /gene="purT"
                     /locus_tag="PLN02_03585"
     CDS             complement(3421..>4437)
                     /gene="purT"
                     /locus_tag="PLN02_03585"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HOI51433.1"
                     /translation="DGAALRRLVELEKPQLIVPEIEAIATDTLVELEAEGLAEVIPTA
                     RAARLTMNREGIRRLAAEELGLPTSPYRFADSLAELQAAIDGGIGYPCIVKPVMSSSG
                     KGQSLLRGPDDVQKAWDYAASGGRVNQGRVIVEGFIDFDYEITLLTVRARDASGEVVT
                     HFCEPIGHIQVSGDYVESWQPQAMSPAALARAQEIAGAVTGNLGGRGLFGVELFVKGD
                     MVWFSEVSPRPHDTGLVTLCSQRFSEFELHARAILGLPVDTALREPGASAVIYGGMEG
                     QGIAFAGVAEALAVPRSDLRLFGKPEAFSKRRMGVAVANGADTDEARSRAKLAASRVR
                     PVKA"
BASE COUNT          796 a         1505 c         1408 g          728 t
ORIGIN      
        1 cgagatcagc atccgcaatc tcaactttta ctatggcaag gccatcgccc tcaagaacat
       61 caacctcgag atctacaagg gcaaggtcac cgctttcatc ggcccctcgg gctgcggcaa
      121 atccacgctg ctgcgcacga tcaaccgcat gtacgacctt tacccggggc agcacgccga
      181 tggcgaactg atcgtcgccg gcaagaacat cctggaaaag ggggtcgata ccatcgaact
      241 gcgcgccaag gtcggcatgg tcttccagaa gccgacgccg ttcccgatgt cgatctacga
      301 gaacatcgcc ttcggcgtgc gcatgcacga gaagctgacg cgcaacgaga tggacgaccg
      361 gatcgaatgg gcgctgacca aggcggcgct gtggaacgag gtcaaggaca agctgaacaa
      421 gcctggcacc ggtctttccg gcggccagca gcaacgcttg tgcatcgccc gcggcgtcgc
      481 cgtcaagccg caggtgctgc tgctcgacga accgacctcg gcgctcgacc cgatctcgac
      541 gatgcgcatc gaggaactga ttcacgaact gaagcgcgag ttcaccatcg ccatcgtcac
      601 ccataacatg cagcaggcgg cgcgggtgtc ggactacacc gcctacatgt acctgggcga
      661 gctggtcgaa ttcggcaaga ccgacgagct gttcatcaag ccgcaggaca agcgcactga
      721 agactacatc accggccgca tgggctgatc ggagaccaga aatgaatgaa accctgcatg
      781 tatccagcca gttcgacgag gatctggcgc gtctgcgcac ccacgtgctg cagatgggcg
      841 gcgttgtcga aacgcagatt tcggcggcga tcgaagccta ttccagcggc gaggtggcga
      901 gcgtcaagac catcgtcgag accgaccgcc ggatcaacca gctcgagcgc tcgatcgacg
      961 aggattgcgc ccacatcatc gcccagcgcc agccggcggc gtccgacctg cgtctggtgc
     1021 tcggcatcag caagatcgtc accgacctcg aacggaccgg cgacgaagcc aagaagatcg
     1081 ccaagagcgt ccgccgcatc tacgaagccg gccatcagcc gacgcaatac ggcatcggca
     1141 tccgccacct tgccgaagcg gtgctggcgc tggtgcgtcg ctcgctcgac gccttcgccc
     1201 gccaggacgc cgcccaggca atcgaggtga tccgcgccga ccaggacgtc gatctcgaat
     1261 tcaagtcgat catccgtcag ttgatcacgc atatgatgga agacccgcgt accattacga
     1321 cttcgctcga aatcatggcc atctcgcggg ccatcgagcg catcggcgat catgccaaga
     1381 acatttgcga acaggtgatc tacgtggttg aaggccgcga catccgtcac gacaaggagg
     1441 cgatcaagtg acaccgacga tactggtcgt cgaagacgaa ccggccatcc aggaactggt
     1501 tgtcatcaac ctcaaacacg cgggctttct cgtcgtccgc gccggcagcg ccgaagaagc
     1561 cgattcggcg attcgcgccg cgctgcccga tctgatcgtc ctcgactgga tgctgcccgg
     1621 ccagtccggc gtcgcgctgg ccaggaagct gcgcgccgac gagcggacgc gcgagattcc
     1681 gatcatcatg ctgaccgccc gcgtccatga ggaagacaag gtgcaggggc tcgacgccgg
     1741 tgccgacgat tacgtgacca aacccttctc gcccaaggaa ctggtggctc gggtgcgcgc
     1801 cgtgctgcgc cgccgtgccc cgcatctggc gggtgaagcg gtcgaggttg gcagcctgca
     1861 gatcaatccg gcgacgcacc gggtggtcgc cgccggccag tcggtcgatc tcggtcctac
     1921 cgagttccgc ttgctgttct tcttcatgac ccacgccgag cgcgtgtaca cccgcgccca
     1981 actgcttgac gaagtctggg gcgaccacgt cttcatcgaa gaacgcacgg tcgatgtgca
     2041 catccgccgc ctgcgcgccg cgcttgagcc gagtggtcat catgagcggg tggagacggt
     2101 gcgcggtacc ggctaccggt tccggggagc ctgagcggcg tgcagcgggc cttgttctat
     2161 ggcgtactga cggcggcggt ggcctttccg ctcggttact tcgtcgaggc ctggatgggc
     2221 tgggtgctgt tctatgccgg cctcagcctg cacctggcga tgcactaccg caacttttcg
     2281 cgcctcgaac ggtggacccg caaaccggtc ctcgacgcca gcctggaagg cgacggcgac
     2341 tgggaccgga tcttccgccg cctctaccgc cacgagaagg agctgctcga gaagatcgaa
     2401 ctgcgcgagc gcgacatcgc ccgcctgatc gccgccgtcc atgcgatgaa cgacggcatc
     2461 gtgctgctcg acggcaactt ctacatccag ttctgcaaca agaccgccga gcgccagctc
     2521 ggtctcgatt cggcgcgcga ccgcggcgcc tcgatcgcca acatcctgcg ccagccggag
     2581 ttcgtcgcct acctcgccga ggccgatttc tcgcgtcccc tgaccttgcg tctcgaccgc
     2641 tacttcgaac gcgtgctctc gctctacctg atcctctacg ccgaggattg ctggctgctg
     2701 caggtcaagg acatcacgca gaccgaccgc ctcgacagca tgcgccgtga cttcgtcgcc
     2761 aacgtctcgc acgagttgcg cacgccgctc accgtgctct ccggttttgt cgagaccctg
     2821 caggagatcg acgtcgacgc cgaatcgcgc aagcactacc tgcaactgat ggggcagcaa
     2881 tcgacgcgca tgctgtcgat cgtccaggac ctgctgaccc tgtcgtcgat cgagtcggcg
     2941 ccgccgccgc aggacgaagt ggtcgacatg gcgaccctgg tcgacaagct tcatcgcgac
     3001 gccgaggcgt tgtccgccgg ccgccacacc atccatctcg aaaccgaggg ccagggcgac
     3061 ctgctcggcg ccgaatcgga actgatcagc gctttcgcca acctggtcgc caacgccgtg
     3121 cgctacacgc cgaacggcgg gacgatcacg ctgagctgga aggtatcgcc cgcaggcgcc
     3181 gaatttgccg tccaggatac cggcatcggc atcgacgcca agcacatccc gcggctgacc
     3241 gaacgcttct accgcgtcga ccgcggccgc tcgcgcgacg ccggcggtac cggcctcggc
     3301 ctggcgatcg tcaagcactc gctgagccgt caccaggcga cgctggaagt gaccagcaag
     3361 ccgggcgagg gcagccgttt cgccgccaag tttccgccca gccgggtgag cggcgtctga
     3421 tcaggccttg accggtcgta cccggctcgc cgccagtttg gcgcggctgc gcgcttcgtc
     3481 ggtgtcggcg ccgttggcga cggcgacgcc catgcgtcgt ttgctgaagg cttccggttt
     3541 gccgaacagg cgcaggtcgc tgcgcggcac ggcgagcgct tcggcaacgc cggcgaaggc
     3601 gatgccctgt ccttccatgc cgccgtagat caccgccgag gcgcccggct cgcgcagcgc
     3661 ggtgtcgacc ggcaggccga ggatggcgcg ggcgtgcagt tcgaactccg agaaacgctg
     3721 cgaacacagc gtcaccagcc cggtgtcgtg cggccgcggg ctgacttccg agaaccagac
     3781 catgtcgccc ttgacgaaga gttcgacgcc gaacaggccg cgaccgccga ggttgccggt
     3841 caccgcgccg gcgatttcct gggcgcgggc cagcgccgcc gggctcatcg cttgcggctg
     3901 ccaggattcg acgtagtcgc cggagacctg gatgtggccg atcggctcgc agaagtgggt
     3961 gaccacttcg ccgctggcgt cgcgggcgcg caccgtgagc agcgtgatct cgtaatcgaa
     4021 atcgatgaag ccctcgacga tcacccgccc ctggttgacc cggccgccgc tcgccgcgta
     4081 gtcccaggcc ttctgcacgt cgtccgggcc gcgcagcagc gactggcctt tgcccgacga
     4141 cgacatcacc ggcttgacga tgcacgggta gccgatgccg ccgtcgatcg ccgcctgcag
     4201 ctcggcgagc gagtcggcga agcggtaggg cgaggtgggt agccccagtt cctcggcggc
     4261 cagacggcgg atgccttcgc ggttcatggt caggcgggcg gcgcgggcgg tcgggatgac
     4321 ttcggccagg ccttcggctt ccagttcgac cagggtgtcg gtggcgatgg cctcgatttc
     4381 ggggacgatc agttgcggct tttccagctc gaccaggcgg cgcagcgcgg cgccgtc
//
LOCUS       DAOSDX010000114         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6929_length_4431_cov_2.708410, whole genome shotgun sequence.
ACCESSION   DAOSDX010000114 DAOSDX010000000
VERSION     DAOSDX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_4431_cov_2.708410"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLN02_03590"
     CDS             complement(<1..890)
                     /locus_tag="PLN02_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOI51434.1"
                     /translation="MSIIEVQVPQLSESVAEGTLASWKKKIGEAVGRDEILIDIETDK
                     VVLEVPSPAAGVLVEIIKGDGETVVSGELIAKIDTAATAGAVAPAAEAPKAAAPAAAP
                     APAAAAPAGTASPAARKILDEKGIAAADVAGSGRGGRVTKEDAVAAQAKPAAAAGPVV
                     AAKSTTSLPVPPTGVALGDRTEERVPMTRLRARIAERLLQSQQTNAILTTFNEVNMAP
                     VMALRKQYGEKFEKTHGVRLGFMGFFVKAAVAALQKFPILNASVDGNDIVYHGYVDIG
                     IAVGSPRGLVVPILRNADQMS"
     gene            complement(916..3747)
                     /locus_tag="PLN02_03595"
     CDS             complement(916..3747)
                     /locus_tag="PLN02_03595"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOI51435.1"
                     /translation="MNDKFDSTLYFGGNAPFVEEVYESYLNDPTSVAPEWRDYFDRLA
                     QMPGFVARDVAHAPVVAAFAELAKDGGFKHSAPASSSAEHRKQSAVGQLVTAYRSLGT
                     RWADLDPLKRLPRPKLDELEPSFYGFTDADLKQTFNVGSLKGLPENAKLSDILETLKQ
                     TYCGTIGVEYMYMTDYNEKRWLQEKLESIRSRPSYTADQKKRLLERLTAAETLERYLH
                     TKYVGQKRFSLEGGESLIVAMDEAIRTGGANGVDEVVVGMAHRGRLNVLANTLGKAPS
                     MLFAEFEGKKKSDLTAGDVKYHLGYSSDVATPGGPCHLTLAFNPSHLEIVNPVVAGSV
                     YARQVRRGEGSKSKVLPILVHGDAAVAGQGVNQEMLNFSQTRGYGVGGVLHIVVNNQV
                     GFTTSDPRDYRSGHYCTDIFKMVDAPIFHVNGDDPEAVALVTQLAIEYRQLFQKDVVV
                     DIICFRKLGHNEQDEPMVTQPLMYKIISKHPGTRKLYGDKLIAEGTLPADGPDQMIAE
                     YRAHLDKGELLYNPVLAGYKHPMTIDWTPFLAKDYIETCDTTVPKAELQRLAQRLSTL
                     PEGFTLHSRVKKIVEDRAAMGEGKLPVDWGMAENLAYASLLVSGYGVRISGEDVGRGT
                     FFHRHAAFHDQNRSSWDSGTYHPLKNLQEKQAGFQCYDSVLSEEAVLAFDYGYASANP
                     YELVIWEAQFGDFVNGAQVVIDQFITSGEAKWGRACGLVMLLPHGYEGQGPEHSSARL
                     ERFMNQAAEMNMEVCVPTTAAQVFHMLRRQAVRMQRKPLVVMSPKSLLRHKDAASSLD
                     ELANGEFQRVIGEVDEIDAKKVKRVVLCCGKVYYDLVNARREKQIKDIAIVRIEQLYP
                     FPSGSLAKELAKYPKATEIVWAQEEPRNQGAWYWFASRNHLDSGLDAKQKLLLVARPA
                     SPSPAVGYLAKHNEQQKALVESALGKIDY"
     gene            complement(3862..>4431)
                     /locus_tag="PLN02_03600"
     CDS             complement(3862..>4431)
                     /locus_tag="PLN02_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOI51436.1"
                     /translation="AGSSGANPFACISAGIACLWGPAHGGANEACLQMLEEIGDVSRV
                     GEYIKRAKDKNDSFKLMGFGHRVYKNFDPRAKLMRKVCNEVLSELGLENDRLFKLAME
                     LEKIALEDEYFVEKKLYPNVDFYSGIVQKAIGIPTEMFTCIFALARSVGWMTQWEEMI
                     SDPEYKIGRPRQLYVGAAKRDVVPLAQRG"
BASE COUNT          803 a         1326 c         1417 g          885 t
ORIGIN      
        1 gacatctggt cggcgttgcg caggatcggc acgaccaggc cgcgcggcga accgacggcg
       61 ataccgatat cgacgtagcc gtgatagacg atgtcgttac cgtcgaccga ggcgttcagg
      121 atcgggaact tctgcagcgc ggcaacagcg gccttgacga agaagcccat gaagccaagg
      181 cggacgccgt gggttttctc gaacttctcg ccgtactgct tgcgcagagc catgaccggg
      241 gccatgttca cttcgttgaa cgtggtcagg atggcgttgg tctgttgcga ctgcagcagg
      301 cgctcggcga tacgggcacg cagacgggtc atcggcacgc gttcttcggt gcgatcaccg
      361 agggcgacgc cggtcggcgg caccggcagc gaggtggtcg acttggcagc gacgaccgga
      421 ccagcagcgg cagccggctt ggcctgggcg gcaacggcat cttccttggt gacgcgaccg
      481 ccgcgaccgg aaccggcgac gtcggcagca gcaatgccct tctcgtcgag gatcttgcgg
      541 gcggccgggc tggccgtgcc agccggagcg gctgcggcag gcgccggagc agcggcggga
      601 gcagcagcct tcggtgcttc agcggccggc gcgacggcgc cagcggtggc ggcggtgtcg
      661 atcttggcga tcagctcgcc ggaaacgacg gtttcgccat cgcccttgat gatttcaacg
      721 agaacgccag ccgccggcga cggcacttcg aggacgacct tgtcggtctc gatgtcgatc
      781 aggatttcgt cgcggccgac cgcttcgccg atcttcttct tccaggaagc cagcgtgcct
      841 tcggcgacgg attcggacag ttgcggaact tggacttcaa tgatgctcat gggtgactcc
      901 actctgcttt cgttattagt agtcgatctt gcccagtgcg gactcgacca atgctttctg
      961 ctgttcgttg tgcttggcca gataccctac cgccggcgac ggcgaggcgg gacgcgcaac
     1021 cagcagcagt ttctgcttgg cgtcgaggcc gctgtccaga tgattacgcg aggcgaacca
     1081 gtaccaggca ccctggttgc gcggttcctc ctgcgcccag acaatttccg tcgccttcgg
     1141 atacttggcc aattccttgg ccagggaacc cgaggggaac ggataaagct gttcgatacg
     1201 gacgatggcg atatccttga tctgcttctc acgacgtgcg ttgaccaggt cgtaatagac
     1261 cttgccgcag cacagcacaa cacgcttgac ctttttcgcg tcgatctcgt cgacttcgcc
     1321 gatgacgcgc tggaattcgc cattggccag ttcgtcgagc gacgacgcgg catccttgtg
     1381 gcgcagcagc gatttcggcg acatcacgac cagcggcttg cgctgcatgc gcacggcttg
     1441 ccggcgcagc atgtggaaaa cctgggcggc cgtggtcggg acgcaaactt ccatgttcat
     1501 ttcggcggcc tggttcatga agcgctcaag gcgagccgac gagtgctccg gaccctgacc
     1561 ttcgtaaccg tgcggcagca gcatgaccag accgcaggcg cggccccact tggcttcacc
     1621 ggaagtgatg aactggtcga tgacgacctg ggcgccattg acgaagtcgc cgaactgggc
     1681 ctcccagatc acgagttcat aggggttggc cgaggcgtaa ccatagtcga aagccagcac
     1741 agcttcttcc gacagcaccg agtcatagca ctggaagcca gcctgctttt cctgcaggtt
     1801 cttcagcgga tggtaggtgc cactatccca actgctgcga ttctggtcat ggaaggcggc
     1861 gtggcggtgg aagaaggtgc cgcgcccgac gtcttcaccg gaaatgcgca cgccgtaacc
     1921 ggaaaccagc aaggaagcgt aggccaggtt ctcggccatg ccccaatcga ccggcagctt
     1981 gccttcgccc atcgccgcgc gatcctcgac gatcttcttg acccgcgagt gcagggtgaa
     2041 gccttccggc agcgtcgaca gacgctgcgc cagacgctgc agttcggcct tcggcacggt
     2101 ggtgtcgcag gtctcgatgt aatccttggc caggaacggc gtccagtcga tcgtcatcgg
     2161 gtgcttgtag ccggccagca ccgggttgta gagcagctcg cccttgtcca ggtgagcgcg
     2221 gtattcggcg atcatctggt ccggaccgtc agccggcagc gtcccttcgg cgatcagctt
     2281 gtcaccgtag agcttgcgcg tgcccggatg cttgctgatg atcttgtaca tcaacggctg
     2341 cgtgaccatc ggctcgtcct gctcgttgtg gccgagcttg cggaagcaga tgatgtcgac
     2401 gacgacatcc ttctggaaga gctgacggta ctcgatggcc aattgggtaa ccagcgcgac
     2461 ggcttccgga tcgtcgccat tgacgtggaa gatcggcgca tcgaccatct tgaagatgtc
     2521 agtgcagtag tgaccggagc ggtagtcgcg cgggtcggaa gtggtgaagc cgacctggtt
     2581 gttgacgacg atgtgcagca cgccaccgac gccgtagccg cgggtctgcg agaagttcag
     2641 catttcctgg ttgacgccct gaccggcgac ggcggcgtcg ccgtgaacca ggatgggcag
     2701 caccttggac ttgctgccct cgccgcgccg gacctggcgc gcgtagaccg aaccggccac
     2761 caccgggttg acgatttcca agtgcgacgg gttgaaggcc agcgtcaggt ggcaggggcc
     2821 gcccggggtg gcaacgtcgg acgagtagcc gaggtgatac ttgacgtcgc cggcggtgag
     2881 atcgctcttc ttcttgcctt cgaattcggc gaagagcatc gacggtgcct tgcccagggt
     2941 attggccagc acgttcaggc ggccgcggtg agccatgccg acgaccactt cgtcaacgcc
     3001 attggcgccg ccggtgcgga tcgcttcgtc catggcgacg atcagcgatt cgccaccttc
     3061 aagcgaaaag cgcttttggc cgacgtactt ggtatgcagg taacgttcga gggtttcggc
     3121 ggcggtcagg cgctcgagca ggcgcttttt ctgatcggcc gtgtaggacg gacgggaacg
     3181 gatgctctcc agcttttcct gcagccagcg cttctcgttg tagtcggtca tgtacatgta
     3241 ctcgacgccg atcgtgccgc agtaggtctg cttcagcgtt tcgagaatgt cgctgagctt
     3301 ggcgttttcc ggcaggccct tgagcgaacc gacgttgaag gtctgcttca ggtcggcatc
     3361 ggtaaaaccg tagaacgacg gctccagttc gtcgagtttc ggacgcggca ggcgcttgag
     3421 cggatcgaga tcagcccagc gggtaccgag cgaacggtag gcagtgacca gttgaccgac
     3481 cgcggattgc ttgcggtgtt cggcggaaga cgaggccggc gcactgtgct tgaagccacc
     3541 atccttcgcc agttcggcaa aggcggcaac gaccggcgca tgcgccacgt cacgggcgac
     3601 gaagcccggc atctgggcca ggcgatcgaa ataatcccgc cattccgggg caacggaagt
     3661 cggatcgttc aggtagcttt catatacttc ttcgacaaag ggtgcgttac cgccaaagta
     3721 aagcgtgctg tcgaacttgt cattcatcgt agtcataagc gtcccctgta tgggttttat
     3781 tttcctgcga tacgcattga tgcgcaccgg tgatgctccg catgtaaaaa agggcggccc
     3841 gcgagccgcc cgtttattgt attagccgcg ctgggcaagc ggtaccacgt cgcgcttggc
     3901 tgcaccaaca tacaactggc gcggacgacc gatcttgtat tccggatcgg agatcatttc
     3961 ttcccactgc gtcatccagc caaccgagcg ggccagcgcg aagatgcagg tgaacatttc
     4021 ggtcgggatg ccgatcgcct tctggacgat gccggagtag aagtcgacgt tcgggtagag
     4081 cttcttctcg acgaagtatt cgtcttccag agcgatcttt tccagttcca tcgcgagctt
     4141 gaacaggcga tcgttttcca gacccagttc cgacagcact tcgttgcaca ccttgcgcat
     4201 cagcttggcg cgcgggtcga agttcttgta gacgcggtgg ccgaagccca tcagcttgaa
     4261 ggaatcgttc ttgtccttgg cgcgcttgat gtattcacca acgcgcgaga cgtcgccgat
     4321 ttcttccagc atctgcaggc aggcttcgtt cgcgccgccg tgcgccggac cccacagaca
     4381 ggcgataccg gccgagatac aggcaaacgg gttggcgccc gacgaaccgg c
//
LOCUS       DAOSDX010000115         4430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6930_length_4430_cov_3.214171, whole genome shotgun sequence.
ACCESSION   DAOSDX010000115 DAOSDX010000000
VERSION     DAOSDX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6930_length_4430_cov_3.214171"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLN02_03605"
     CDS             complement(<1..903)
                     /locus_tag="PLN02_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOI51437.1"
                     /translation="MNQNAPAQRIIKIRRDYNTWAANETLEDYALRFTPRSFRKWSEL
                     RVANTAFGAASFLVLEAVGATMLVNAGFINAFWAILATGLIIFLLGLPISHTAAKHGL
                     DMDLLTRGAGFGYIGSTITSLIYASFTFIFFALEAAIMAYALELAFGLPPAWGYLVCA
                     LVVIPMVTHGVTAISRFQAWTQPLWLFLLVLPYVFVLIEEPDAFAGLLAYAGEKGNGG
                     SFNPHLFGAALTVGIAMVTQMGEQVDYLRFMPEKTPANRGRWWLGVIVGGPGWVLPGI
                     LKMLGGALLAWLVLRNGFSPDKAVD"
     gene            1175..4210
                     /locus_tag="PLN02_03610"
     CDS             1175..4210
                     /locus_tag="PLN02_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOI51438.1"
                     /translation="MPGLPRRGFLHPDVLPWIVLGLLLALTFGAWYLAQETANRRASE
                     RFLYRAEKERDYIVQRMSAHLQVLRGAAAFFEASDQVSRDEWRRYVDRLQLGGNLHGA
                     QGLGLALLVTSVDKTAFEERLREDGISPHEIRPPGGREQYSVVTYLEPQNERNRRGIG
                     YDMLIDAARRAAMERARDSGLPALTDKVILKQEPSGPQRQPGFMIYLPVYRTDLPRNT
                     VEQRRAALVGFVFSPFRAGDLLREIMASRERDIDLALFDVTVAPDNLLYDTRADGEAG
                     LAGRHVVELPLELGGHRWVARLQSHHEFDVASTSSLPESIAGYGVLLGLMIFGVLFGN
                     SRYSRRIEAIAERLRESEQSLRSVLDNSPDAVFIARPDGHYEYVNQKASEFVGYTSAE
                     LLAMDIGGLSPNGLIEAHHRIFQRIVGEGHCFTEIELRRKDGTSAFAEMSAVLLPNGN
                     VLGLCRDIARRRQAEQALLAAERKFRGLIEQSLVGVYIIQDGRFPYVNPRFAQMFGFA
                     SPEEVIDRVAVSDLVAPEARETVAENLRRRVSGEVEAINYSFVGQRRDGSRLDVEVYG
                     RIMEHEGRPAVIGVLLDVSERKRVEAELEQNRLHLEELVAARTSELSVAKEVAEAANR
                     AKSSFLANMSHELRTPMNAIIGLAGILQRRYDDPALTDKLDKIAAAAKHLLHLLNDIL
                     DLSKIDAERLTLEQLPFPFGSILADVESLVHERVLARQLTFERRIDSRLASLELLGDP
                     LRLQQILLNLVTNAVKFTEQGGVAIAVRCEAETPADIVLRLSVEDTGIGIPPDAQRRI
                     FEPFEQADSSTTRRHGGTGLGLAICKRLVELMGGGIAVESTPGVGSRFSFVLRFAKVD
                     AATPRSASATIGGSEAEVVIRQHYRDRRVLLVEDDPINQEVARELLGESLGLAVDVAS
                     DGAEAVSKAGASSYDLILMDIQMPIMDGLTATQAIRQLSGHAATPILAMTANAFVDDR
                     RRCLAAGMNDFITKPVDPDVLFLVLMRWFERSGEDGGGR"
     gene            complement(4243..>4430)
                     /locus_tag="PLN02_03615"
     CDS             complement(4243..>4430)
                     /locus_tag="PLN02_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51439.1"
                     /translation="PGVAFCLRQRYTPPLATFFFPQKRKYARASGAENSASKTKAAHR
                     STRESNGSATQAARAAQ"
BASE COUNT          779 a         1354 c         1475 g          822 t
ORIGIN      
        1 gtcgaccgcc ttgtccggcg aaaaaccgtt gcgcagcacc agccaggcga gcagcgcgcc
       61 gcccagcatc ttgaggatgc cgggcagcac ccagcccggc ccgccgacga tcacccccaa
      121 ccaccaacgg ccccggttgg cgggcgtctt ctcgggcatg aagcgcaggt aatcgacctg
      181 ttcccccatc tgggtcacca tggcaatgcc gacggtcagt gctgcaccaa ataggtgcgg
      241 gttgaaactg ccgccgttgc ctttctcgcc ggcataggcg agcagcccgg cgaaggcgtc
      301 gggttcctcg atcagcacga agacgtaggg cagcaccagc aggaagagcc acagcggctg
      361 cgtccacgcc tggaagcggc tgatcgcggt gacgccgtgg gtgaccatgg ggatcaccac
      421 cagcgcgcag accaggtagc cccaggccgg cggcaagccg aaggcaagct ccagcgcata
      481 ggccatgatc gctgcttcga gggcgaagaa gatgaaggtg aacgaggcgt agatcagcga
      541 ggtgatggtc gagccgatgt agccgaaacc ggcgccgcgc gtcagcaggt ccatgtccag
      601 gccgtgcttg gcggcggtgt gcgagatcgg caggccgagc aggaagatga tcagcccggt
      661 cgccaggatc gcccagaagg cgttgatgaa gccggcgttg accagcatcg tcgcgccgac
      721 cgcttcgagc accaggaaag aggccgcgcc gaaggcggtg ttggcgacgc gcagctccga
      781 ccacttgcgg aaactgcgcg gcgtgaagcg cagcgcgtag tcttcgagcg tctcgttggc
      841 ggcccaggtg ttgtagtcgc ggcggatctt gatgatgcgt tgggcggggg cgttctggtt
      901 catgcaccag attgaagcag gttctgtgcc gtcgattttc cgccgtctgt tcggggcgtc
      961 tatcccattc gtgatactgg acgagagccc tttggcgtaa gacacttgcg tcgttggctg
     1021 gcgtatcgcg gcagtcgtcg ccactccgtt tctggtgctt tagaagtctt tttgaatcag
     1081 tgtcttggcg ttttactggc gtgcttccga aacggcccgc ctagtgactg gagcaaattt
     1141 gcccatccat tccgtcagtt cgcgagagtc atcgttgccg ggcctgccgc ggcggggttt
     1201 cctgcatccc gatgttctgc cctggatcgt cctcggcttg ctcctggcgc tgaccttcgg
     1261 cgcctggtat ctggcgcagg aaacagccaa ccgccgcgcc agcgaacgct tcctctaccg
     1321 cgccgagaag gagcgcgatt acatcgtgca gcggatgtcc gcgcatctgc aggtgctgcg
     1381 cggagcagcc gctttttttg aagcgagcga tcaggtcagc cgcgatgaat ggcggcgcta
     1441 tgtcgaccgc ctgcaactgg gcgggaatct gcacggcgcg caagggcttg gtctggcgct
     1501 gctggtgacg tccgtggaca agactgcgtt cgaggagagg ctaagggagg acggcatctc
     1561 gccccatgaa atccggccgc cgggggggcg cgagcaatac agtgtcgtca cctatctcga
     1621 accgcagaac gaacgcaacc ggcgggggat aggctacgac atgctgatcg atgccgcgcg
     1681 gcgcgcggcc atggagcggg cgcgcgacag cgggctgccg gcgctgaccg acaaagtgat
     1741 cctcaagcag gaaccgagcg gcccgcagcg gcaaccgggc ttcatgatct acctgccggt
     1801 gtatcgcacc gatctgccac gcaataccgt cgagcagcgg cgagccgctc tggtcggctt
     1861 tgttttcagt cccttccggg cgggcgatct gctgcgcgag atcatggcca gtcgggaaag
     1921 ggatatcgat ctggccttgt tcgacgtgac ggtggcgccg gataacctgc tctacgacac
     1981 ccgggccgac ggcgaggcag ggctggctgg ccgccatgtc gtcgaattgc cgctggaact
     2041 gggcggtcac cgctgggttg cacgcttgca gagtcaccac gagttcgatg tcgcatcaac
     2101 cagcagcttg ccggagagca tcgccggtta cggggtgttg ctgggcttga tgatcttcgg
     2161 cgtgctcttc ggcaattccc gctacagccg ccggatcgaa gcgatcgccg agcgcttgcg
     2221 cgaaagcgag cagtcattgc gcagcgttct cgacaactcg ccggatgcgg ttttcatcgc
     2281 ccgcccggac ggccattacg agtacgtcaa ccagaaggcc agtgaattcg tcggctacac
     2341 gagcgccgag ctgttggcca tggatatcgg cgggctctcc ccgaatggcc tgatcgaggc
     2401 gcaccaccgg atcttccagc gtatcgtcgg cgaagggcac tgttttaccg agatcgaact
     2461 gcggcgcaag gatggtacga gcgccttcgc cgaaatgagc gcggtcctgc tgccgaacgg
     2521 caacgtcctc ggcctgtgcc gcgacatcgc ccggcgccgc caggcggagc aggcgctgct
     2581 cgccgccgag cgcaagttcc gtggcctgat cgagcaatcg ctggtcggcg tctacatcat
     2641 ccaggacggg cgttttccct acgtcaatcc gcgctttgcc cagatgttcg gctttgccag
     2701 cccggaggag gtgatcgacc gcgttgccgt cagcgatctg gtcgccccgg aagcccggga
     2761 aaccgtcgcc gaaaacctgc gccggcgggt cagcggcgaa gtcgaggcga tcaattattc
     2821 cttcgtcggc cagcgccggg acggtagcag gctggatgtc gaggtctatg ggcggatcat
     2881 ggagcacgag ggccggccgg ccgtcatcgg cgtcctgctc gacgtttccg aacgcaagcg
     2941 ggtggaagcg gaactcgaac agaaccgcct gcatctggaa gagctggttg ccgcccgcac
     3001 cagcgaactg tcggtggcga aggaggtcgc cgaagccgcc aaccgggcaa aaagcagttt
     3061 cctcgccaac atgagtcacg aactgcgcac gccgatgaac gccatcatcg gtctggccgg
     3121 catcctgcag cgccgctacg acgacccggc gctgaccgac aaactggaca agattgccgc
     3181 tgcggccaag catctgctgc atctgctcaa cgacattctc gacctctcca agatcgacgc
     3241 cgaacggctg acgctggagc agctgccttt ccccttcggc agcatcctcg ccgatgtcga
     3301 gagcctggtc cacgagcgag tgctggcgcg gcaactgact ttcgagcgcc gcatcgattc
     3361 ccgcctcgcc agtctggaac tgctgggcga ccccctgcgc ctgcagcaga tactgctgaa
     3421 cctggtaacc aatgccgtga agttcaccga gcagggcggc gtggccatcg cggttcgctg
     3481 cgaggcggaa actcctgccg acatcgtgtt gcgcttgagt gttgaggata ctggcatcgg
     3541 cattccgccc gatgcccagc ggcgtatttt cgaacccttc gagcaggccg acagttcgac
     3601 cacccgccgc catggcggca ccggcctcgg tctcgccatc tgcaagcggc tggtcgagtt
     3661 gatgggcggc gggattgcgg tggaaagcac gccgggcgtc ggcagccgct tcagcttcgt
     3721 gctgcgcttc gccaaggtcg acgccgcaac gccgcgaagt gcgtcggcaa ctatcggcgg
     3781 cagcgaagcc gaagtcgtca ttcgacagca ttatcgggat cggcgtgtcc tgttggtcga
     3841 ggacgacccg atcaaccagg aagttgcccg cgagctgctc ggggaatcgc tcggacttgc
     3901 cgtcgatgtc gcctcggatg gcgccgaggc ggtgagcaag gcaggcgcct cgtcctatga
     3961 cctgatcctg atggacatcc agatgccgat catggacggt ctgaccgcga cacaggcgat
     4021 ccgccagctt tccgggcatg cggcaacgcc gattctggcg atgaccgcca atgccttcgt
     4081 cgatgaccgt cggcgttgtc tggccgcggg gatgaacgat ttcatcacca agccggttga
     4141 tccggatgtg ctttttctgg tgttgatgcg gtggtttgag cgaagcgggg aggacggagg
     4201 ggggcgttga ggtgattgct tccgtggggg ttgaggcttg ttctattgcg ctgcgcgggc
     4261 ggcttgggtt gctgaaccgt tgctttctct tgtgcttcgg tgggcggctt tggtttttga
     4321 tgcgctgttt tccgcgcctg acgcgcgggc gtactttctt ttttgtggaa aaaagaaagt
     4381 agccaaaggc ggggtgtatc gctgccgcag gcaaaaagcc accccaggtt
//
LOCUS       DAOSDX010000116         4417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6962_length_4417_cov_3.308803, whole genome shotgun sequence.
ACCESSION   DAOSDX010000116 DAOSDX010000000
VERSION     DAOSDX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6962_length_4417_cov_3.308803"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLN02_03620"
     CDS             <1..489
                     /locus_tag="PLN02_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOI51440.1"
                     /translation="AHYTADDWQVFGELAYDFAAGRTQLAPFADLAFAGLRREAFSEK
                     GGGAFNLAGRAGSESVATGSLGLRAAQDVELGASKGQWSASLAWQHAAGDLTPESRLA
                     FAGGARFVNAGVPLARNALAVAASLRVALSPTARLGIDYTGQFAAGRREHGVGASLAV
                     EF"
     gene            complement(499..1059)
                     /locus_tag="PLN02_03625"
     CDS             complement(499..1059)
                     /locus_tag="PLN02_03625"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HOI51441.1"
                     /translation="MTTDIEEIHRAMREADCLATAAEVAVALDRMAGEITTALADSNP
                     LVYTVMNGGLIFAGRLLSRLHFPLETSYLHASRYGQQLKGNSLLDWRVRPTQDLKGRT
                     VLVLDDILDEGHTLQAICQHLRDEGAARVLTAVMVHKVHDRKAVPGMRADFSGLDIAD
                     RYLFGCGMDYKGYWRNAPGIYALKGH"
     gene            complement(1084..1962)
                     /gene="menA"
                     /locus_tag="PLN02_03630"
     CDS             complement(1084..1962)
                     /gene="menA"
                     /locus_tag="PLN02_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOI51442.1"
                     /translation="MNPSTAWFLACRPKTLTVSLSPVLVGTAIGWHEAGQLLWLPLLA
                     AAFGAALIQIGTNLFNDVGDYLRGIDRPGRFGPRRATAEGWLTPNAVRVGAWISFALA
                     FLCGVYLVAHAGWPIVIIGLCSLYAGWSYTGGPRPIAYGPFGEFFVLVFFGIVAVGGS
                     HYLQVPAWSALALLAGSLLGLHAAAVITVNNYRDLDTDRDAGRRTLAVRLGRPATCRL
                     YIAEMLLPYLLLALLAAPLGAYAALPLLTLPLALLLCRRFLCTPPGPAFNGILAATAA
                     HQLGFAALLALAFIRP"
     gene            complement(2031..3161)
                     /locus_tag="PLN02_03635"
     CDS             complement(2031..3161)
                     /locus_tag="PLN02_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOI51443.1"
                     /translation="MTEPVATVDTLIIGAGPVGMTLQLALAAGGRQALLLDRRAPAAL
                     QADPRALALSHGARELLETLGAWPAKAATPIETIHISQRGGFGRTLIDRSEHDLPALG
                     YVVRYRDLASALAARLAPDALRAEVEILAITPDRDGVTVALREQGVSRRLRARTLVHA
                     EGTPWDDPSTLLGDYEQHAVICEVTPTPGHARRAWERFTPDGPLALLPLGDEYSIVFT
                     LPPAKADAVMALDDAGFIAALEAQFARPMRFARPGQRSRYPLALRMRDTLVREREVWV
                     GNSAQTLHPVSGQGFNLGLRDAWQLAQVLLDGDDLSRYSSLRRLDRQGGAFFTDKVVR
                     AFSNDFPPLRAARGLGLLALDLCPAARRFVAKRMIWGARAWP"
     gene            complement(3154..4416)
                     /locus_tag="PLN02_03640"
     CDS             complement(3154..4416)
                     /locus_tag="PLN02_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOI51444.1"
                     /translation="MLQTIGDGVAIIPTAPEVVRNRDAHYPYRFDSYFWYLSGFPEPE
                     AVIVLVGGKRPKSILFCRDKHEEREIWDGYRYGPKAAKSAFGFDAAYSIEQLDKKLPE
                     LLVDRDTLWHAVGHDDAWDARIARALNAVRAQARAGKRAPRAIHDLRAELDAMRLYKD
                     EAELATMQRAADIASAGHARAMRACRPGMAEYELEAELSYEFRKRGADGHAYTPIVAG
                     GANACVLHYVSNDKLLNDNTLVLIDAGCEVQGYAADITRTFPVGGRFNAAQKDVYEIV
                     LAAQAAAIAATGPGRHFMEAHDAAVRVLTRGLVDLKLLTGDIDTLIEKGDFRRFYMHR
                     TGHWLGLDVHDAGEYKIGNDWTTLQPGMTITVEPGLYIRPGADVPTELAGIGIRIEDD
                     VRVTPSGCAVYTTAPKTVAEIEEVMRHD"
BASE COUNT          657 a         1386 c         1688 g          686 t
ORIGIN      
        1 gctcactaca cggccgacga ctggcaggtc ttcggcgaac tggcttacga cttcgccgcc
       61 ggccggacgc agctggcgcc gtttgccgac ctggccttcg ccggtctacg gcgcgaggcg
      121 ttcagcgaaa agggcggcgg cgcgttcaat ctggcggggc gtgccggcag cgaatcggtt
      181 gccactggct cgctggggct gcgcgccgcg caggatgtcg aactgggcgc aagcaaggga
      241 caatggtcgg ccagtctggc ctggcagcat gcggccggcg acctgacgcc ggaaagccgc
      301 ctggccttcg ccggcggagc gcgtttcgtc aatgccgggg tgccgctggc gcgcaatgcg
      361 ctggccgtgg cggccagcct gcgggtggcg ctgtcgccga cggccaggct ggggatcgat
      421 tacaccgggc aattcgctgc cgggcgccgc gaacacggcg tcggcgccag cctcgccgta
      481 gagttctgag ccgcccgctc agtgcccctt gagcgcgtag atgcccggcg cgttgcgcca
      541 gtagcccttg tagtccatgc cgcagccgaa caggtaacgg tcggcgatgt cgaggccgga
      601 aaagtcggcg cgcatgccgg gcacggcctt gcggtcgtgc accttgtgca ccatcaccgc
      661 ggtcagcacg cgggcggcgc cttcgtcgcg caggtgctgg cagatcgcct gcagcgtgtg
      721 gccttcgtcg aggatgtcgt cgaggaccag gacggtgcgc cccttcagat cctgggtcgg
      781 ccgcacgcgc cagtcgagca ggctgttgcc tttcagttgc tggccgtagc ggctggcgtg
      841 caggtaggag gtttcgagcg ggaagtgcag gcgcgacagc agccggccgg cgaagatcag
      901 gccgccgttc atcaccgtgt acacaagcgg gttgctgtcg gccagcgcgg tggtgatctc
      961 gccggccatg cggtcgagcg cgacggcgac ttcggccgcc gtcgccaggc agtcggcttc
     1021 gcgcatggcg cggtggattt cttcgatgtc ggtggtcatg tggtctcctg gcagggcgaa
     1081 gggtcagggg cggatgaagg ccagcgcgag cagcgcggcg aaacccagtt ggtgcgccgc
     1141 ggtggccgcg aggatgccgt tgaaggccgg gccgggcggc gtgcagagaa agcggcggca
     1201 gagcagcagg gcgagcggca gggtgagcag gggcagggcg gcgtacgcgc cgagcggtgc
     1261 agcaagcagg gcgagcagca ggtagggtag caacatttcc gcgatgtaga ggcggcaggt
     1321 ggcggggcgg ccgaggcgga ccgccagcgt gcggcgaccg gcgtcgcggt cggtgtcgag
     1381 gtcgcggtag ttgttgacgg tgatcaccgc cgccgcgtgc aggccgagca ggctaccggc
     1441 gagcagggcg agcgccgacc aggccggtac ctgcaggtag tggctgccgc cgacggcgac
     1501 gatgccgaag aagacgagga cgaagaattc gccgaacggg ccgtaggcga tcggccgcgg
     1561 tccgccggtg taggaccagc cggcgtagag cgaacagagg ccgatgatga cgatcggcca
     1621 gccggcatgg gcaacgagat agacgccgca gaggaaggcc agcgcgaagc tgatccaggc
     1681 accgacgcgc acggcgttcg gcgtcagcca gccttcggcg gtggcgcgcc gcgggccgaa
     1741 gcggccggga cggtcgatgc cgcgcaggta gtcgccgacg tcgttgaaca ggttggtgcc
     1801 aatctggatc agcgccgcgc cgaaggctgc ggcaagcagc ggcagccaga gcagttgtcc
     1861 ggcttcatgc cagccgatgg cggtgccgac aagtaccggc gacagcgata cggtcagggt
     1921 cttcgggcgg caggcgagaa accaggcggt actggggttc atgcggggct tcggtgacgg
     1981 acagcccgaa ctttaacccg aatttcccgc cgcggcggcg cctgggcggg tcatggccag
     2041 gcgcgggcgc cccagatcat gcgcttggcg acgaagcggc gcgccgccgg gcagaggtcg
     2101 agggcgagca ggccgaggcc gcgcgccgcg cgcaagggcg ggaaatcgtt ggaaaacgcg
     2161 cgcacgacct tgtcggtgaa gaatgcgccg ccctggcggt caaggcggcg caggctggag
     2221 taacgactca ggtcgtcgcc gtcgagcagc acttgcgcca gttgccaggc gtcgcgcagg
     2281 ccgaggttga aaccctggcc ggaaaccggg tgcagggtct gggcgctgtt gccgacccac
     2341 acttcgcgtt ccctgaccag ggtgtcgcgc atgcgcagcg cgagcggata gcggctgcgt
     2401 tggcccgggc gggcaaagcg catggggcgg gcaaactgcg cttccagcgc ggcgatgaag
     2461 ccggcgtcgt cgagtgccat caccgcgtcg gccttggccg gcggcagggt gaacacgatc
     2521 gagtattcgt cgcccagcgg cagcagggcg agcgggccgt ccggcgtgaa gcgttcccag
     2581 gcgcgcctgg cgtggcccgg cgtcggtgtc acttcgcaga tcaccgcatg ctgttcgtag
     2641 tcgccgagca gcgtgctcgg gtcgtcccaa ggcgtgcctt cggcgtgtac cagagtgcgc
     2701 gcgcgcagac ggcggctgac gccctgttcg cgcagggcga cggtgacgcc gtcgcggtcc
     2761 ggcgtgatgg cgaggatttc cacttcggcg cgcagcgcgt ccggggccag acgggcggcc
     2821 agcgcgctgg ccaggtcgcg gtagcggacg acgtaaccga gcgccggcag gtcatgttcg
     2881 ctgcggtcga tcagggtgcg gccgaagccg ccgcgttgcg agatatggat cgtctcgatc
     2941 ggcgtcgccg ccttcgccgg ccaggcgccg agcgtttcca gcagttcgcg cgcgccatgc
     3001 gacagcgcca gggcgcgcgg gtcggcctgc aatgcggctg gcgcccgccg gtcgagcagc
     3061 agcgcttgcc ggccgccggc ggcgagcgcc agttgcagcg tcatgcctac cgggccggcg
     3121 ccgatgatca gggtatcgac ggttgcgacg ggttcagtca tggcgcatca cctcctcgat
     3181 ttcggcaacc gtcttcggcg ccgtggtgta aaccgcgcaa ccggatggtg tgacgcgcac
     3241 gtcgtcctcg atgcggatgc cgatgccggc cagttcggtc ggcacgtcgg cgcccgggcg
     3301 gatgtacagg ccgggttcga cggtgatggt catgcccggc tgcagcgtcg tccagtcgtt
     3361 gccgatcttg tattcaccgg catcatgcac atccaggccg agccagtggc cggtgcggtg
     3421 catgtagaaa cgacggaaat cgcccttttc gatcaaagtg tcgatgtcgc cggtcagtag
     3481 tttcagatcg accaggcctc gcgtcagcac gcgcacggcg gcgtcgtgcg cttccatgaa
     3541 gtggcgcccg ggcccggtgg cggcgatggc ggcggcctgg gcggccagca cgatctcgta
     3601 cacatccttc tgcgccgcat tgaagcgccc gccgaccggg aaggtgcggg tgatgtcggc
     3661 agcgtagccc tgcacctcgc agccggcgtc gatcaggacc agcgtgttgt cgttgagcag
     3721 cttgtcgttg ctgacgtaat gcagcacgca ggcgttggcg ccgccggcga cgatcggcgt
     3781 gtaggcatgg ccgtcggcgc cgcgcttgcg gaactcgtag gacagctcgg cttccagttc
     3841 gtactcggcc atgcccggcc ggcaggcgcg catggcgcgg gcgtggccgg cgctggcgat
     3901 gtcggcggcg cgctgcatgg tggccagttc ggcctcgtcc ttgtagaggc gcatggcgtc
     3961 gagttcggcg cgcagatcgt ggatcgcgcg cggcgcccgc ttgccggcac gggcctgtgc
     4021 gcgcaccgcg ttcagcgccc tggcgatgcg cgcatcccag gcgtcgtcgt ggccgacggc
     4081 gtgccatagc gtgtcgcggt cgaccagcag ttcgggcagt tttttgtcca gttgttcgat
     4141 cgagtatgcg gcgtcgaagc cgaaggcgct tttcgccgct ttcgggccgt agcggtagcc
     4201 gtcccagatt tcgcgttctt cgtgcttgtc gcggcagaac aggatggatt tcggccgttt
     4261 gccgccgacc agcacgatca ccgcttccgg ttccgggaaa cccgacaagt accagaagta
     4321 gctgtcgaaa cggtagggat agtgcgcgtc gcggttgcgc acgacctccg gcgccgtcgg
     4381 gatgatggcg acgccgtcgc ctatggtctg cagcagg
//
LOCUS       DAOSDX010000117         4413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_6974_length_4413_cov_4.408444, whole genome shotgun sequence.
ACCESSION   DAOSDX010000117 DAOSDX010000000
VERSION     DAOSDX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6974_length_4413_cov_4.408444"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1897
                     /locus_tag="PLN02_03645"
     CDS             377..1897
                     /locus_tag="PLN02_03645"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOI51445.1"
                     /translation="MIYAAPGAAGAKIAYKAKYDNFINGQWTAPVKGQYFDVVTPVNG
                     KVYTQVARSTAEDIELALDAAHAAFPKWGKTDAATRSNILLKIADRLEANLELLAYAE
                     TVDNGKPIRETLAADIPLAVDHFRYFAGCLRSQEGGISEIDENTMAYHIHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALGAGNCVVLKPAESTPISILILVDLIADLLPPGVLNIVNG
                     LGREAGMPLATSKRIAKIAFTGSTATGRVIAQAAANNLIPATLELGGKSPNIFFADVM
                     DKDDGFLDKAIEGLVLFAFNQGEVCTCPSRALIQESIYEKFMEKALKRVAAIKQGSPL
                     DTETMMGAQASQIQMDKIESYLKLGKEEGAEVLIGGERAHLGGDIEGGYYIQPTLFKG
                     HNKMRIFQEEIFGPVLAVTTFKDEAEALAIANDTLYGLGAGVWSRNGNVAYRMGRAIQ
                     AGRVWTNCYHAYPAHAAFGGYKESGIGRETHKVMLDHYQQTKNLLVSYAEQKLGFF"
     gene            2009..3160
                     /gene="yiaY"
                     /locus_tag="PLN02_03650"
     CDS             2009..3160
                     /gene="yiaY"
                     /locus_tag="PLN02_03650"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HOI51446.1"
                     /translation="MAQSAFFIPAVNLMGEGCLETAVQSLGGYGYRRALIVTDSWLEK
                     AGVAARVAGLLEQAGIGAEIFAGAQPNPTIGNVEAGLARLRAADCDCVVSLGGGSPHD
                     CAKGIALVAANGGSIADYEGVDRSAKPQLPLVAINTTAGTASEMTRFCIITDESRHIK
                     MAIVDRHVTPLLSVNDPLLMLAKPPALTAATGMDALTHAVEAYVSTAATPITDACALK
                     AVTMIAANLRQAVADGQNVAAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVEAFNASVASERLADVAAAMGVAESGADSAANAQRCLEAIRRLAADV
                     GIPRGLVDLGVKEADIPTLAANALKDACGLTNPRRADQADIEAIFRAAL"
     gene            3245..>4413
                     /locus_tag="PLN02_03655"
     CDS             3245..>4413
                     /locus_tag="PLN02_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOI51447.1"
                     /translation="MISIVAYRVVSEGSTRSLISQRVGELTQTNTVESLKNLAWAHAG
                     VIQSKFDLALNAARTMAHTFEVGKKSGGLGRDQINDVLYHVLVNNPEFNGTYSNWEPN
                     ALDGKDAAFQNGRDGNNAQTGRFTPYWNRGQDGKIAVQPLVEYDTMDRHPNGVLKGGW
                     YIGPRENHQESVLDPFPYIVQGKSVWLTTLSVPIMLNQRFVGVAGTDYNLDFVQKLAV
                     QADQELFEGKGGVTIISNAGLVVADSEHPERIGQAFSASKSADDSELLKNVQEGRAKG
                     WFDESNKHMKAVAPITLGRTGKPWAVLVSVPQDVVLAQAQALDQEIQARGTSSTIWQI
                     LVGLVVTAVATALLWFVAGNIARPIRAAADFARAVLAGDFSRRFQHELNDEVGQLA"
BASE COUNT          875 a         1469 c         1353 g          716 t
ORIGIN      
        1 caggcgcgtg tcgctcgaga acaggttgcg cacgaggtcg atggacatgg cggtggcggc
       61 ggaatgggaa gccggaagga tagcgaagac atcgtcatgt gacaacggga ttgttgttgc
      121 aagcctgcta tccaagcctg cccggccact gctccacatt tgaacagccg ttgatcaatg
      181 gagcagcccg gcccccggca ggcgcccgaa caaaccgcac aagccattga ttaaaaagcg
      241 ctttgaaaaa ccgggaacgg gctggcacgc ggcgtgcgaa tgataggtca cgagtcacgg
      301 cgcagcgtcg atcgcgaggc aacgccggat ttccaaagac ttgtccgcaa cccatttcga
      361 gaacactgga gacaaaatga tctacgcagc ccccggcgcc gctggcgcaa aaatcgccta
      421 caaggccaag tacgacaact tcatcaacgg ccaatggacc gcgccggtca agggccagta
      481 cttcgacgtg gtgaccccgg tcaacggcaa ggtgtacacg caggtcgccc gctccaccgc
      541 cgaagacatc gagctggcgc tcgacgccgc ccacgccgcc ttcccgaagt ggggcaagac
      601 cgatgccgcg acccgctcca acatcctcct gaagatcgcc gaccgcctcg aagccaacct
      661 cgaactgctc gcctacgccg aaaccgtcga caacggcaag ccgatccgcg aaaccctggc
      721 tgccgacatc ccgctcgccg tcgaccactt ccgctacttc gccggctgcc tgcgctcgca
      781 ggaaggcggc atctcggaaa tcgacgagaa caccatggct tatcacatcc atgagccgct
      841 cggcgtcgtt ggccagatca ttccgtggaa cttccccatc ctgatggccg cctggaagct
      901 cgccccggcg ctcggcgccg gcaactgcgt ggtcctcaag ccggccgaat cgacgccgat
      961 ctcgatcctg atcctggtcg acctgatcgc cgatctgctg ccaccgggcg tcctgaacat
     1021 cgtcaatggt ctgggtcgcg aagccggcat gccgctggct accagcaagc gcatcgccaa
     1081 gatcgccttc accggctcca ccgccaccgg ccgcgtgatc gcccaggccg ccgccaacaa
     1141 cctgatcccg gccaccctcg aactgggcgg caagtccccg aacatcttct tcgccgacgt
     1201 gatggacaag gacgacggct tcctcgacaa ggcgatcgaa ggcctggtcc tgttcgcctt
     1261 caaccagggc gaagtctgta cctgcccgag ccgtgcgctg atccaggaat cgatctacga
     1321 gaagttcatg gaaaaggcgc tgaagcgcgt cgccgcgatc aagcagggct cgccgctcga
     1381 caccgaaacg atgatgggcg cccaggcctc gcagatccag atggacaaga tcgagtccta
     1441 cctcaagctg ggcaaggaag aaggcgccga agtgctgatc ggtggcgaac gtgcccacct
     1501 cggcggcgac atcgaaggcg gttactacat ccagccgacg ctgttcaagg gccacaacaa
     1561 gatgcgcatc ttccaggaag aaatcttcgg gccggtgctc gcggtgacca ccttcaagga
     1621 cgaagcggaa gccctggcga tcgccaacga cacgctctac ggcctcggcg ccggcgtctg
     1681 gagccgtaac ggcaacgtcg cctaccgcat gggccgcgcc atccaggccg gccgcgtgtg
     1741 gaccaactgc taccacgcct acccggccca cgccgctttc ggcggctaca aggaatccgg
     1801 catcggccgc gagacgcaca aggtcatgct cgaccactac cagcagacca agaacctgct
     1861 cgtctcctac gcggaacaga agctcggctt cttctaaacc agccaccgac gcccgccttg
     1921 cgcgggcgtt ttgccaacta ccgaccggtc agcgggttca aggggaacct cgcgcggtcg
     1981 gatcatcatt tcacggagaa caagcaaaat ggcacaaagc gcatttttca ttcccgccgt
     2041 caacctgatg ggcgaaggct gtctcgagac ggccgtccag tccctgggag gatacggtta
     2101 ccggcgggcg ctgatcgtta ccgatagctg gctggagaaa gccggcgtcg ccgcccgggt
     2161 ggcgggtctg ctggaacagg ccgggatcgg tgccgagatt tttgccggcg cccagcccaa
     2221 cccgaccatc ggcaacgtcg aagccggcct ggcgcgtctg cgcgctgccg attgcgattg
     2281 cgtcgtctcg ctcggcggcg gttcgccgca cgattgcgcc aagggcatcg cgctggtcgc
     2341 cgccaatggc ggcagcatcg ccgattacga aggcgtcgac cgctcggcca agccgcagtt
     2401 gccgctcgtt gcgatcaaca cgacggccgg cacggccagc gaaatgacgc gcttctgcat
     2461 catcaccgac gagtcacgcc acatcaagat ggccatcgtc gaccgccacg tgacgccgct
     2521 gctttcggtc aacgatccgc tgctgatgtt ggccaagccg ccggcactga ccgcggcgac
     2581 cggcatggac gcgctgacgc acgccgtcga ggcctatgtc tccaccgcgg cgacgccgat
     2641 caccgacgcc tgcgccctga aggcggtgac gatgattgcc gccaatctgc gccaggcggt
     2701 cgccgatggc cagaacgtcg ccgcccgcga ggccatggct tacgcccagt tcctcgccgg
     2761 catggccttc aacaacgcct cgctcggcta cgtgcacgcc atggcgcacc agctcggcgg
     2821 cttctacaac ctgccgcatg gcgtctgcaa cgcggtgctg ctgccgcacg tcgaggcttt
     2881 caatgcctcg gtggcgagcg agcgtctggc cgatgttgcg gcagcgatgg gcgtggcgga
     2941 aagcggcgcc gacagtgcgg cgaacgccca gcgctgcctc gaggcgatcc gccgcctggc
     3001 tgccgatgtc ggcattccgc gcggcttggt cgatcttggc gtcaaggaag ccgacatccc
     3061 gaccctggcc gccaacgcgt tgaaggacgc ctgcggcctg accaatcccc ggcgcgccga
     3121 ccaggccgac atcgaagcga tcttccgcgc cgccctgtaa gtctgttgaa aaggtgccgt
     3181 tgaagtcatg aagtcaattc agggaaggat atcgttcctg gcaggcgcct gcctgctcgg
     3241 gtcggtgatc agcatcgtcg cttacagagt ggtttccgaa ggaagcacgc gcagcctgat
     3301 ttcgcagcga gtcggcgagc tgactcagac aaatacggtc gagagtctga aaaacctcgc
     3361 ctgggcgcac gccggggtga ttcagagcaa gttcgacctg gcgctcaatg ccgcccgcac
     3421 catggcccac actttcgagg tgggcaagaa gagcggtggg ctggggcgcg atcagatcaa
     3481 cgatgttctt tatcacgtcc tggtcaacaa ccctgaattc aacggcacct attcgaactg
     3541 ggagcccaac gcgctggacg gcaaggatgc tgccttccag aacggcaggg atggcaacaa
     3601 cgcccagacc gggcgcttca ccccgtactg gaatcgcggt caggacggca agatcgccgt
     3661 tcaaccgctg gtcgagtacg acaccatgga ccgtcacccc aacggcgtgt tgaagggggg
     3721 ctggtacatc ggtccgcgcg agaatcacca ggaaagcgtg ctcgacccgt tcccgtacat
     3781 cgttcagggc aagtcggtgt ggctgaccac cctgtcggtg ccgatcatgc ttaaccaacg
     3841 cttcgtcggc gttgccggta ccgactacaa cctcgatttc gtgcagaaac ttgccgtcca
     3901 ggccgaccag gagctgtttg aaggcaaggg cggggtcacc atcatcagca acgccggcct
     3961 ggtcgtggcc gacagcgagc atccggaacg tatcgggcag gctttttcgg cgagcaagtc
     4021 cgccgatgac agcgaactgc tcaagaatgt ccaggaaggt cgggccaagg gctggttcga
     4081 cgaaagcaac aagcacatga aggcggtcgc gccgattacc ctcggccgca ccggcaagcc
     4141 ctgggcggtg ctggtgagcg tgccgcagga cgtggtgctc gcccaggccc aggcgcttga
     4201 tcaggaaatc caggcgcgcg gtacctcaag cactatctgg cagatcctgg tcggcctggt
     4261 ggtcaccgcc gtggcgacag ccttgctgtg gttcgtcgcc ggtaatatcg cgcgtccgat
     4321 ccgagccgcc gcggattttg cccgggccgt gctggccggc gatttttcgc gccgcttcca
     4381 gcacgaattg aacgatgagg tcgggcaact cgc
//
LOCUS       DAOSDX010000118         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7050_length_4378_cov_2.913023, whole genome shotgun sequence.
ACCESSION   DAOSDX010000118 DAOSDX010000000
VERSION     DAOSDX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7050_length_4378_cov_2.913023"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLN02_03660"
     CDS             complement(<1..426)
                     /locus_tag="PLN02_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOI51448.1"
                     /translation="MKFPPSASAALTALRQGETLPSALLEECIARIERHNPAINALVT
                     LDLASARASAARCDQYLAAHGFPAEQPLFGLPISVKDAFATASLRTTSSHPPLRDHVP
                     TADATVVARLRAAGAILVGKSNLSQLAGDPQCWSPLFGPT"
     gene            complement(468..953)
                     /locus_tag="PLN02_03665"
     CDS             complement(468..953)
                     /locus_tag="PLN02_03665"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HOI51449.1"
                     /translation="MSLPSISRLAKDAERYPFQDCRRVPLAGAPDASQLLDAFAHAAP
                     PWVDALMRLRNGLVAPFGLKTGTLAEVGPPYVVGQHIGLFRVIHLDQLEAILGEDDRH
                     LDFRISMFCHDGALWGCTLVRPHNRFGRLYLALVKPWHHLIAGAMFRRMAARLNAAVK
                     P"
     gene            complement(1048..1881)
                     /locus_tag="PLN02_03670"
     CDS             complement(1048..1881)
                     /locus_tag="PLN02_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI51450.1"
                     /translation="MHHNLAPLTPALRPLFGELLQIRLPGGPYQLPATLQPTLVCSAS
                     GGLSVADEYGGWRQMPLVFLAGATTGVHRVRAEPDTEMLLLAVRSGQLPRLFGPSAGS
                     LLDRNVALEDLLPAGLAEALSDGLRENTQAARRQETLERQLLRCHAAHAHRQPDLQLP
                     EAWLFRPLAEIAAAFTLSERQFERRFRSSTGQSLRAFRQQFRWSRLVREVVFGRGQAP
                     NWVDAAYGGGYADQSHLHRDFRRYTGHTPGELGRRLGNGNPELWPYHLNAAEIGRLFA
                     G"
     gene            complement(2040..3029)
                     /gene="rlmF"
                     /locus_tag="PLN02_03675"
     CDS             complement(2040..3029)
                     /gene="rlmF"
                     /locus_tag="PLN02_03675"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HOI51451.1"
                     /translation="MHPRNKNADGYDLAALTATSAALAKYLKTTPAGEPTIDFANPAT
                     VKALNRALLMHHYGVKGWEIPEGYLCPPIPGRADYLHALADLLATCNKKAVPTGPGVR
                     ALDIGTGANLVYPLIGHAEYGWSFLGVDIDEAALANAQKILAKNPELAGSIELRHQTV
                     WDNIFTGLLRSGESFDVSLCNPPFHNSPDEVLAVSQRKWNNLGKPGAKKGSTQPRLNF
                     GGGGTELWCNGGERAFVKSMIEQSTQIPKRVLWFTSLLSKADNLVHIEAALKKAKVVE
                     SRILPMTQGQKQSRIVAWTFCANGEREKWRRERWSLATDTVSAATPTDSAPAD"
     gene            3190..>4378
                     /locus_tag="PLN02_03680"
     CDS             3190..>4378
                     /locus_tag="PLN02_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI51452.1"
                     /translation="MKLSIERRHIEPLLAAFPRLAHLREQLRFGNRVEVSFAQLEAAE
                     LDMLQAQYAAGDRELRAQAAQIATLRAALAETGHRFAAEELEMAVPAIARYLADGSER
                     GWLFSAAITGKPLAWVVARLDYTPPSEEETGKIFLELRANSRAKMATATLRISGPDIA
                     GHTVAEILAGKGFVKETPALLAAYEEGAARYFEWRAQYGAQFSGSGTGWFAENPTATH
                     RDTDYSRKDQIVLSSTGSPARLVNDEGILGERVLAMDATGDILAKFVRRVQRNSRLSG
                     KEEAEVEETRDGIAKGLFTELPVHFFILMFHLDLHHYVWVHVDDIAPYAYQPELKDKL
                     VLPPEQTDLIDILTAEMDVLMDDIIAGKSGGTTVLCAGPPGVGKTLTAEVYSEIIRRP
                     LYRV"
BASE COUNT          709 a         1309 c         1592 g          768 t
ORIGIN      
        1 cgtggggccg aacagcgggc tccagcattg cgggtcgccg gccagttgcg acagattgct
       61 cttgccgacc aggatggcgc cggcggccct gaggcgggcg acgacggtgg catcggctgt
      121 cggcacatga tcgcgcaggg gcgggtggct gctggtggtg cgcaggctgg cggtggcaaa
      181 ggcatccttg accgagatcg gcaggccgaa caagggctgt tcggcaggaa agccgtgcgc
      241 cgccagatat tggtcgcaac gcgctgcgct ggcccgggcg ctggccaggt cgagggtgac
      301 gagggcgttg atcgccgggt tatggcgttc gatgcgggcg atgcattctt ccagtagcgc
      361 cgacggcagt gtttcgccct gtcggagggc ggtgagggcg gcgctggcgc tgggcgggaa
      421 tttcaatttt tggttttttt cccggttgac cgtggaatcc tcggcgttca tggcttgacg
      481 gcagcattca ggcgcgcggc catgcggcgg aacatcgcgc cggcgatcag atggtgccag
      541 ggtttgacca gcgccaggta caggcggcca aaacggttgt gcgggcggac gagcgtgcag
      601 ccccagagag cgccgtcgtg gcagaacatg ctgatgcgaa agtcgagatg ccggtcgtcc
      661 tcgccgagga tggcttcgag ctgatcgagg tgaatgacgc gaaagagtcc gatgtgctgg
      721 ccgacgacgt agggtgggcc gacttcggcc agcgttccgg tcttcaggcc gaacggcgcg
      781 acgagcccgt tgcgcaggcg catcagggcg tcgacccagg gtggcgcggc gtgggcaaag
      841 gcgtcgagca actggctggc gtcgggcgcg ccggccagcg gtacgcgccg gcaatcctgg
      901 aaggggtagc gctcggcatc cttggcaaga cgggaaatgg acggcaggga catggcaggc
      961 tccggcaatg atgttggcgg gactgtagtc caggcgcgtc gccagcgtct tgaaccgaaa
     1021 cgacattgcc gggcgtcatc cgccggctca tccggcaaag agccggccga tttccgcagc
     1081 gttgaggtgg tagggccaga gttccgggtt gccgttaccc aggcgccgtc cgagttcgcc
     1141 tggcgtatgg ccggtgtagc ggcggaaatc gcgatgcagg tgcgactggt cggcgtagcc
     1201 gccaccgtag gcggcatcga cccagttcgg cgcttgcccc cggccgaata cgacttctcg
     1261 cacgagccgg ctccagcgaa actgctggcg gaatgcgcgc aggctttgtc cggtgctgct
     1321 gcggaagcgg cgttcaaatt ggcgctcgct cagcgtgaag gccgcagcaa tctcggccag
     1381 ggggcgaaac agccacgcct ccggcaactg caggtcgggt tggcgatggg catgggcggc
     1441 atgacagcgg agcagttgcc tttccagtgt ttcctggcgc cgggcggcct gagtgttctc
     1501 gcgcagcccg tcgctcagcg cttcggccaa tcctgcaggc agcaagtctt ccagcgcgac
     1561 attccggtcg aggaggctgc cggcagaagg cccgaagagg cgaggcaact gtccggagcg
     1621 aacggcgagc agcagcatct cggtgtccgg ctcggccctg acgcggtgaa cgccggttgt
     1681 tgcacccgcc aggaagacca gcggcatctg ccgccagccc ccatactcat cggcgacgct
     1741 gagcccgccg ctggccgagc agaccagggt cggttgcaag gtcgcgggca actgatacgg
     1801 gcctcccggc aggcgaatct gcagcaactc cccgaacagg gggcgcaagg cgggcgtcag
     1861 gggggccagg ttgtgatgca tcgccagact cggaaagggg atatttgcta cgcattttga
     1921 atgcagtcct gagcggcagg caagtgatcc tcttgtcagg gcgccattcg gccgggagct
     1981 gcttgggcgg gcgaaatcgc caaactgcgg aacggcgtaa gcaaatccgt cagcgcgtcc
     2041 tagtcggcag gggcggaatc tgtcggggtt gctgcggaga ccgtgtcggt ggccagcgac
     2101 caccgttccc gccgccattt ctcccgttcg ccgttggcgc agaaggtcca ggcgacgatg
     2161 cggctttgtt tctggccctg ggtcatcggc aggatgcgcg attcgacgac tttcgccttt
     2221 ttcagcgcgg cttcgatgtg taccaggttg tcggctttcg agagcagcga ggtgaaccac
     2281 agcacgcgct tcgggatctg cgtgctttgc tcgatcatcg atttgacgaa ggcgcgttcg
     2341 ccgccgttgc accagagttc ggtgccgccg ccgccaaagt tgaggcgcgg ttgggtgctg
     2401 cccttcttgg cgccgggttt gcccaggttg ttccatttgc gctggctgac ggcgaggact
     2461 tcgtcgggcg agttgtggaa gggcgggttg cacagcgaga cgtcgaagct ttcgccgctg
     2521 cgcagcaggc cggtgaagat gttgtcccag accgtttggt ggcgcagttc gatgctgccg
     2581 gcgagttccg ggttcttggc gaggattttc tgggcgttgg cgagcgccgc ttcgtcgatg
     2641 tcgacgccga ggaaggacca gccgtattcg gcgtggccga tcagcggata gaccaggtta
     2701 gcgccggtgc cgatgtcgag cgcgcgcacg ccggggccgg tcggaacggc tttcttgttg
     2761 caggtggcga gcaggtcggc cagcgcgtgc aggtagtcgg cgcggccggg gatcggcggg
     2821 cacaggtagc cttccggaat ctcccagccc ttgacgccgt agtggtgcat gagcaacgcg
     2881 cggttgagcg ccttgaccgt tgccgggttg gcgaagtcga tggtcggctc gccggccggc
     2941 gtggtcttca ggtatttggc gagcgcggcc gaggtcgcgg tgagtgcggc gaggtcgtag
     3001 ccgtcggcat tcttgttgcg cgggtgcatg gtgatctttc cgggaacggg gcgcgattgt
     3061 cccacgtgtt gcaggtgcga caaaatcgtc ggcgcgcaag cgcatgattt ttctggtgtt
     3121 tggcgggcga gcggcaaggc atgaaactgg cttgacgaag cgacaagcca atctccgggg
     3181 aatccgccga tgaagttgtc gatcgaacgc cgccacattg aaccgctgct cgcagccttt
     3241 ccgcgcctgg cgcacttgcg cgagcaattg cgtttcggca accgggttga ggtttccttc
     3301 gcccagctgg aagctgccga actcgacatg ctgcaagcgc agtacgccgc cggcgaccgc
     3361 gagttgcgcg cgcaggcggc gcagattgcg acgctgcgcg cggcgctggc cgagaccggg
     3421 catcgctttg cggccgagga gctggaaatg gcggtgccgg ccatcgcgcg ctacctggcc
     3481 gacggcagcg aacgcggctg gctgttctcg gcggcgatca ccggcaagcc gctggcctgg
     3541 gtggtggcgc ggctcgatta cacgccgccg tcggaggagg aaacaggcaa gattttcctc
     3601 gaactgcggg ccaactcgcg ggcgaagatg gcgacggcga ccttgcgcat ttccggcccc
     3661 gacatcgccg gccacacggt ggccgaaatc ctcgccggca agggtttcgt caaggagacg
     3721 ccggccttgc tggcggccta cgaagaaggc gcggcacgct atttcgagtg gcgggcgcag
     3781 tacggcgcgc agttttccgg cagcggcacc ggctggttcg ccgagaaccc gacggcgacg
     3841 caccgcgaca ccgattattc gcgcaaggac cagatcgtcc tttcgtccac cggctcgccg
     3901 gcacgcctgg tcaacgacga gggcatcctc ggcgagcgcg tgctggcgat ggacgccacc
     3961 ggcgacatcc tggccaagtt cgtccgtcgc gtgcagcgca actcgcggct gtccggcaag
     4021 gaggaggcgg aagtcgagga gacccgcgac ggcatcgcca agggcctgtt cactgaactg
     4081 ccggtgcatt tcttcatcct gatgttccac ctcgacctgc accattacgt ctgggtgcat
     4141 gtggacgaca tcgcgcccta cgcctaccag ccggaactga aggacaagct ggtgctgccg
     4201 ccggagcaga ccgacctgat cgacatcctg accgccgaga tggacgtgct gatggacgac
     4261 atcattgccg gcaagtcggg cggcacgacg gtgctctgcg ccgggccacc gggggtgggc
     4321 aagacgctga ccgccgaggt gtactcggaa atcatccgcc gcccgctcta ccgcgtgc
//
LOCUS       DAOSDX010000119         4372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7058_length_4372_cov_3.029882, whole genome shotgun sequence.
ACCESSION   DAOSDX010000119 DAOSDX010000000
VERSION     DAOSDX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7058_length_4372_cov_3.029882"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLN02_03685"
     CDS             complement(<1..786)
                     /locus_tag="PLN02_03685"
                     /inference="COORDINATES: protein motif:HMM:NF025014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOI51453.1"
                     /translation="MNKTSFRYLQLAASLTLALPALLIAPAAVAEALPASGEIELAAG
                     GQRLVDTPKTVSRLAVGNEAVAAVKVIGSRQFVIQGNQPGVTTLMVWQRGEKTADRYP
                     LRVNKAVALPPEGAGELQLKAAGDLSVLSGTASDLAAQAVAREYAAHGAGDAKPAVVD
                     RSVMGFGGEVQIDVRVVEFSRKILNKAGLSILSNKSGFTFGSYAPGSLSKAQLSNGAI
                     SVEAELPINQALNLLVGNAKKNLLGVVSVLEGNGFARTLARPTL"
     gene            complement(783..1661)
                     /gene="cpaB"
                     /locus_tag="PLN02_03690"
     CDS             complement(783..1661)
                     /gene="cpaB"
                     /locus_tag="PLN02_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOI51454.1"
                     /translation="MLIVAAIGLAAYAWVLSKDMAAQQPAPMQQVVVAAQRIPAGTLL
                     TAQHLQLMAFPSRPAGSYAAVEAVLGKTVPVDLAAGEPLLAERIEGPATPSLKQLQPG
                     ERAVAIKVDEVVAVGNRLAPGDLVDVFATFRRNNEEIADSQARLLLSGLRILAFGGLD
                     APEAKKTGSTMRNAAETPRTAVLAVPLADVERLALAAEAGRLLLALRPLDAATAAAPE
                     AATPGQAAPAALLSAPAQAVSLRELAGKVGAVPLPAKPSAGVAGRPAAPAPTVKVMHG
                     LKETSVQVSNRNGAGQ"
     gene            complement(1723..2154)
                     /locus_tag="PLN02_03695"
     CDS             complement(1723..2154)
                     /locus_tag="PLN02_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOI51455.1"
                     /translation="MSQRAGRSAQRGAVVIEFALVFVLFFIVMYGIVAYGVIFAVKHS
                     LVQAANEGARAAVRDVGGVTARVELARTVAAESLAWLGDKAPEPQVSAALCAATPYVC
                     LKVELAYDYATNPLVPALPALGVALPERLTGQAMVQLDAVY"
     gene            complement(2170..2649)
                     /locus_tag="PLN02_03700"
     CDS             complement(2170..2649)
                     /locus_tag="PLN02_03700"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOI51456.1"
                     /translation="MSLAAQLLLSFWAGALAFADWRHRRLPNAWLVLGLAFGVMHLAV
                     RGVMPFGGSPFDAAMASLAALLCFLPLYAAGWMGAGDVKFLAVIGWLGGFPVLAGALL
                     YGSLLAGGLALLLLQPGGLPGVRVAGVLRGRIPYGSCLAVALLPQLWGWLDFGSVLP"
     gene            complement(2698..2862)
                     /locus_tag="PLN02_03705"
     CDS             complement(2698..2862)
                     /locus_tag="PLN02_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOI51457.1"
                     /translation="MWDSIKKFVKDEEGATAVEYGLIAALIAVAIILSVTDVGTKLKA
                     VFDSIKDALP"
     gene            complement(2937..3302)
                     /locus_tag="PLN02_03710"
     CDS             complement(2937..3302)
                     /locus_tag="PLN02_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI51458.1"
                     /translation="MKALQRVLIVEDQADLRLLIRLTLEPLGVELREVANGMEALLAA
                     RSFKPDVLILDVMLPGGVDGYQICQAIKQDPLTRATRVIMVSARGQKSDIERGYQAQA
                     DRYLVKPFSPAQLLKEVGA"
     gene            complement(3299..>4372)
                     /locus_tag="PLN02_03715"
     CDS             complement(3299..>4372)
                     /locus_tag="PLN02_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOI51459.1"
                     /translation="LLRSNRERDAILTLGPDGFVFADEQGRVSFVNPAFAGMTGLAAG
                     LVVGRPLAELDLHIAQLGGGSPGFAEVGEGADGLLQLERPHKTVIRWLARLIPDGAGG
                     IHGRVIYLRDITRESEVDRMKSDFLSTAAHELRTPMASIFGFSELLLSREFEPAMQRD
                     LLERIHRQTRNLINLVNELLDLSRIEARGGKSFKMQVQEITPVVLNAVAAQYVPAPTH
                     KLEFDWPKDLPKVRIDAEKFQQCLINVLSNAVKYSPGGGVIRVNALRRGEAESACIGV
                     AISDPGIGMTPEQSARIFERFYRADPSGAILGSGLGMSLVKEYMAVFGGDVEVVSAPG
                     QGTTVVLWLPAADALGESGGSGR"
BASE COUNT          754 a         1575 c         1367 g          676 t
ORIGIN      
        1 cagcgtcggc cgcgccaggg tgcgggcgaa accgttgcct tcgaggacgc tgacgacgcc
       61 gagcaggttc ttcttggcgt tgccgaccag caggttgagc gcctggttga tcggcagttc
      121 ggcctcgacg ctaatcgcgc cgttgcttag ctgtgccttg ctcaggctgc ccggcgcgta
      181 gctgccgaaa gtgaagcccg acttgttgga aaggatgctg agaccggcct tgttgaggat
      241 cttgcggctg aactcgacga cccggacgtc gatctgcact tcgccgccga agcccatcac
      301 gctgcggtcg accaccgccg gcttggcgtc gccggcaccg tgcgccgcgt actcgcgagc
      361 caccgcctgc gctgccagat cgctggcggt gcccgacagc acgctgaggt cgccggccgc
      421 cttcagctgc agttcgccgg cgccttccgg cggcagcgcc accgccttgt tgacgcgcaa
      481 cgggtagcgg tccgccgtct tttcgccgcg ctgccagacc atcagcgtgg tgacgccggg
      541 ctggttgccc tgaatcacga actggcggct gccgatcacc ttgaccgcgg ccaccgcttc
      601 attgccgacc gccagccggc tgaccgtctt cggcgtatcg accaggcgct gcccgccggc
      661 ggcaagctcg atctcgccgc tcgccggcag ggcctcggcg acggcggccg gagcgatcag
      721 cagcgccggc aacgccagcg tcaggctggc agccaattga aggtagcgga aactcgtctt
      781 gttcattgcc cagccccatt ccgattactg acctgcacgc tggtctcctt gagcccgtgc
      841 atcaccttga ccgtcggtgc cggcgccgcc ggcctgcccg cgacgccggc cgacggcttg
      901 gccggcagcg ggacggcgcc aaccttgccg gccagctcgc gcaggctgac cgcctgcgcc
      961 ggcgccgaga gcaaggcggc gggcgccgcc tggccgggcg tcgcggcttc cggcgccgcc
     1021 gccgtggcgg catcgagcgg acgcagcgcg agcagcaggc ggccggcctc ggcggccagc
     1081 gccagccgtt cgacgtcggc cagcggtacg gcgaggacgg cggtacgcgg cgtctcggcg
     1141 gcattgcgca tggtgctgcc ggtcttcttc gcttccggcg cgtcgagccc gccgaaggcc
     1201 aggatgcgca gtccggacag caacaggcgg gcctggctgt cggcgatttc ctcgttgttg
     1261 cggcggaagg tggcgaagac atcgaccagg tcacccggcg ccagccggtt gccgacggca
     1321 accacttcat cgaccttgat cgccaccgcc cgctcgccgg gctgcaactg cttcagcgac
     1381 ggcgtcgccg gcccttcgat gcgttcggct agcagcggct cgccagcggc aaggtcaacc
     1441 ggcacggtct tgccgagcac cgcctcgacc gccgcatagc tgccggccgg gcgcgacgga
     1501 aacgccatca gttgcagatg ttgcgccgtc agcagcgtgc cggcgggaat ccgctgcgcc
     1561 gccaccacca cctgctgcat cggcgccggc tgctgcgccg ccatgtcctt gctgagcacc
     1621 catgcatacg ccgccagccc gatggccgca acgatcagca atgcccccac tatcctttgc
     1681 gtcttgccca atttgcctta ctccctgagg actccaaagc aatcaataaa ccgcgtcgag
     1741 ctgtaccatg gcctgcccgg tcagccgttc cggcagcgca acccccagtg ccggcagcgc
     1801 cgggaccagc ggattggtgg cgtagtcgta ggccagctcg accttcaggc agacgtaggg
     1861 cgtcgccgcg cagagcgccg cgctgacctg cggctcgggt gccttgtcgc cgagccaggc
     1921 gaggctttcg gcggccaccg tgcgcgccag ctcgacgcgc gccgtgacac cgccgacatc
     1981 gcgcaccgcc gcccgcgccc cttcgttggc ggcctgcacc agggagtgct tgacggcaaa
     2041 gatgacgccg taggcgacga tgccgtacat gacgatgaaa aacagcacga acaccagcgc
     2101 gaactcgatg accaccgccc cgcgctgggc actccgaccc gccctctgcg acaccatgca
     2161 ctcccccgac tacggcagca cggacccgaa atccagccac ccccacaact gcggcagcag
     2221 cgccaccgcc agacagcttc cgtagggaat ccgcccccgc aacacacccg ccacccgcac
     2281 ccccggcaag ccacccggct gcagcagcag caaggccagc cccccggcca gcagcgagcc
     2341 gtacaacaag gccccggcca gcaccgggaa accgcccaac cagccgatga cggccaggaa
     2401 cttgacgtcc cccgccccca tccagcccgc tgcatagagg ggcagaaaac agagcagcgc
     2461 cgccagcgaa gccattgccg catcaaacgg tgaaccgccg aaaggcatga cgccgcgtac
     2521 cgccagatgc atcaccccga aagccagacc gagcaccagc caggcgttcg gcagacgccg
     2581 gtggcgccag tcggcaaagg ccagcgcccc cgcccagaaa ctcaacaaca attgcgcagc
     2641 cagcgacacg cgcccgctct caagaaacgg acgagacgcc gacgccggaa cgatccatca
     2701 gggcagggcg tccttgatcg aatcgaaaac tgctttcagc ttggtaccga cgtcggtcac
     2761 cgaaagaatg atcgccacgg cgatcagggc ggcaatcagc ccgtactcga cagcggttgc
     2821 accttcttca tctttaacga atttcttgat cgaatcccac atgacacgct ccataaaatt
     2881 ttcaaaaaaa tatgatttat tcatttgccg caattattct ctttccagca tgattgtcaa
     2941 gccccgacct ccttgagtag ctgcgccgga ctgaagggtt tcaccaggta acgatcggcc
     3001 tgcgcctggt agccgcgttc gatatcgctt ttctggccac gggcgctgac catgatcacc
     3061 cgcgtcgctc gggtcagcgg gtcctgcttg atcgcctgac agatctggta gccatcgaca
     3121 ccaccgggca gcatgacgtc gaggatcagc acgtccggct tgaagctgcg cgccgccaac
     3181 agcgcctcca tgccgttggc cacctcgcgc agttcgacgc cgagcggctc cagggtcagg
     3241 cggatgagca ggcgcaggtc ggcctgatcc tcgacgatca atacccgttg caatgccttc
     3301 accgtccgct cccgccagat tcgcccagag cgtcggccgc cggcagccag aggacgacgg
     3361 tcgtcccttg cccgggcgcg ctgaccacct cgacatcgcc accgaaaacc gccatgtatt
     3421 ccttgaccaa ggacatgccg agaccggaac cgaggatggc gccactcgga tcggcgcggt
     3481 agaagcgctc gaagatgcgt gcgctctgct ccggcgtcat gccgatcccc gggtcgctga
     3541 tggcgacgcc gatgcaggcg ctttccgcct cgccgcggcg cagcgcgttg acccggatga
     3601 cgccgccgcc cggcgaatac ttgacggcat tgctcagcac gttgatcagg cactgctgga
     3661 acttctcggc gtcgatcctg actttgggca gatccttcgg ccagtcgaac tccagcttgt
     3721 gcgtcggcgc cggcacgtac tgcgccgcta ccgcgttgag cacgaccggg gtgatttcct
     3781 ggacctgcat cttgaagctc ttgccgccgc gcgcctcgat ccgcgacagg tcgagcagtt
     3841 cgttgaccag gttgatcagg ttgcgcgtct ggcgatggat gcgctcgagc aggtcgcgct
     3901 gcatggccgg ctcgaactcg cgcgacagca gcagttcgga gaaaccgaaa atgctcgcca
     3961 tcggcgtccg cagctcatgc gccgcggtgg acaggaaatc gctcttcatc cggtccacct
     4021 cgctttcgcg ggtgatgtcg cgcaggtaga tgacgcggcc gtggatgccg ccggcgccgt
     4081 cggggatcag ccgggccagc cagcggatca ccgtcttgtg cgggcgttcg agctggagca
     4141 ggccgtcggc gccttcgccg acttcggcaa aacccggcga gccaccgccc aactgggcga
     4201 tgtgcaggtc gagttcggcc agcggccgac cgaccaccag cccggcggcc aagccggtca
     4261 tgccggcgaa ggcgggattg acgaaactga cccgcccctg ttcgtcggcg aagacgaagc
     4321 catccggccc cagggtgagg atcgcatcgc gttcgcggtt gctgcgcagc aa
//
LOCUS       DAOSDX010000120         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7150_length_4330_cov_3.746901, whole genome shotgun sequence.
ACCESSION   DAOSDX010000120 DAOSDX010000000
VERSION     DAOSDX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7150_length_4330_cov_3.746901"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PLN02_03720"
     CDS             <1..1122
                     /locus_tag="PLN02_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HOI51460.1"
                     /translation="KTAAGTPISGRPVPAGAVGNSGEIPLTDAFTYDATRAGYDSNGL
                     DPESLIYDSANGVFWTSDEYGPFIAKIDAATGKIVQKYQPGTAAGDLPAVLAHRRANR
                     GMEGLTMSGGVLHGFLQSPIDPFDAKGKSIEAVDVSDLDEDGKTSDKVKVKDFARFAR
                     WLAFDPATGTSHLYAYPLDYAIAGEKWSKNRSGSAKLGDVVALGNNRFLVIEQGADAN
                     GAVRNFLMLVELPANATDIAGDGYGLEMNSIDGSTLTDTTRSWASVVPLRKRLLVDLN
                     ALGWKAEKAEGLTLVDGQTIGLINDNDFGLRSILVDAAGNEVDGSPEDCTLDASTGVL
                     SNCPNGATGARVTRGKASERPTRLWLLKLPQALSSYTMN"
     gene            1207..2616
                     /locus_tag="PLN02_03725"
     CDS             1207..2616
                     /locus_tag="PLN02_03725"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOI51461.1"
                     /translation="METAPALVWFRRDLRDHDHAALAAALASGRPVHCAFVFDRDILD
                     ALPSRVDRRVEFILGSLQQLDAALRRRGGGLIVRHGRAGDELPRLAGELGVTEVYANR
                     DYEPAAQARDAAVDRQLAAAGGRLRLYKDQVIFERDELLTGAGRPYTVFTPYKNAWLK
                     RLSAADHAPFSAQGRLAPAPESLPTLAELGFAPTDLGQLGIVPGMAGARQLWDEFRAG
                     RIRRYGTLRDFPALKGVSYLSVHLRFGTISIRELVSAALAEKADPWLNELIWRDFYFM
                     ILDRFPHVVDRAFKPEYDAIRWADWPEGLAAWQQGRTGYPLVDAAMRQLNHSGWMHNR
                     LRMVVASFLCKDLGLDWRLGERYFAERLNDFDLAANNGGWQWAASSGCDAQPYFRIFN
                     PVTQSEKFDPDGKFIRRYVPELARVPDKYIHAPWTMGRLEQEALGVVIGRDYPGPIVD
                     HAAAREKTLARYAVVKKAV"
     gene            complement(2657..>4330)
                     /locus_tag="PLN02_03730"
     CDS             complement(2657..>4330)
                     /locus_tag="PLN02_03730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOI51462.1"
                     /translation="RRQLREIEIQAETQMQARVAHTPEGQEEFDPLELDRFTRFQELT
                     RFMAESVNDVATVQQNLFKNLDDANAAILAQARLNRGLQQELMGVRMLPFASQAERLY
                     RIVRQTAKEVGKRANFDIVGGQVEIDRSVLEKIMAPIEHMLRNSVTHGIETREQRAAA
                     GKPETGEVTLKLAQEANEIVLSLSDDGRGLDRQRIRARAEEMGLLQPGQEVADAALLD
                     FIFRPGFSTATEVTQVAGRGVGMDVVRNAVIELGGRIEIVSEAGHGSTFRLYLPLTLA
                     VTQTLLVRAGSTVYAVPSTMIEQVLEVKEKGLAEIRDAGAADWQGNHYPFHFLPRLLG
                     DAEALPETRRRYWILLLRAGSQRIALLVDELQGNQEVVVKNIGSQLARVAGIAGATVL
                     GDGRVVLILNPVALAARAPLIAVAEVAAPAVVAAPLPVAAPHLPTVMVVDDSLTVRKI
                     TSRLLLREGYQVVVAKDGVDALEQLIDALPDVILSDIEMPRMDGFDFVRNLRADERLR
                     DIPVVMITSRTADKHRNYALEIGANHYLGKPYDEDELLGLLAGYCGKST"
BASE COUNT          718 a         1489 c         1429 g          694 t
ORIGIN      
        1 aagaccgccg ccggcacgcc gatcagcggc cgcccggtac cggccggcgc ggtcggcaac
       61 tcgggcgaga ttccgctcac cgacgccttc acctacgacg ccacgcgcgc cggctacgac
      121 agcaacggcc tcgacccgga aagcctgatc tacgacagcg ccaacggcgt cttctggacc
      181 tccgacgagt acggcccctt catcgccaag atcgacgcgg cgaccggcaa gatcgtccag
      241 aaataccagc caggcactgc cgccggcgac ctgccggcgg tgctcgccca tcgtcgcgcc
      301 aaccggggca tggaaggcct gaccatgagc ggcggcgtcc tgcacggttt cctgcagagc
      361 ccgatcgatc cgttcgacgc caagggcaag tcgatcgagg cggtcgacgt cagcgacctc
      421 gacgaggacg gcaagaccag cgacaaggtc aaggtcaagg acttcgcccg tttcgcccgc
      481 tggctcgcct tcgacccggc gaccggcact tcccacctct acgcctaccc gctcgattac
      541 gcgattgccg gcgaaaagtg gtccaagaac cggagcggca gcgccaaact gggcgatgtc
      601 gtggccctgg gcaacaaccg cttcctggtc atcgaacagg gcgccgacgc caacggtgcg
      661 gtccgcaact tcctgatgct ggtcgaactg ccggccaacg cgaccgatat cgcgggcgac
      721 ggttacggtc tggaaatgaa cagcatcgac ggcagcacgc tgaccgacac cacgcgtagc
      781 tgggccagcg tcgtgccgct gcgcaagcgc ctgctggtcg atctcaacgc actcggctgg
      841 aaggccgaga aggccgaggg gctgaccctg gtcgacgggc agacgatcgg gctgatcaac
      901 gacaacgatt tcggcctgcg ctcgatcctg gtcgacgccg ccggcaacga agtcgatggc
      961 agcccggagg actgcacgct cgacgcctcg accggcgtcc tgagcaactg cccgaacggc
     1021 gccactggcg cccgcgtcac ccgcggcaag gcatccgagc gaccgacccg gctgtggctg
     1081 ctgaagctgc cgcaagcctt gagcagctac acgatgaact gaagccgccg acagagcggt
     1141 tcactttcgg ccaccccgcg cgggtggcct ttttcttgga catgcgccgg ccactataat
     1201 cggccgatgg aaaccgcccc cgccctcgtc tggttccgcc gcgacctgcg cgaccacgac
     1261 cacgccgccc tcgccgccgc cctggccagc ggccggccgg tgcattgcgc cttcgtcttc
     1321 gatcgcgaca tcctcgacgc gctgcccagc cgcgttgacc gccgtgtcga gttcattctt
     1381 ggcagcctgc agcaactcga cgccgcgctg cgtcgccgcg gcggcgggct gatcgtccgc
     1441 catggccgcg ccggcgacga gcttccccgg ctcgccggcg aactgggcgt caccgaggtc
     1501 tacgccaacc gcgactacga accggcggcg caggcccgcg acgccgccgt cgaccggcaa
     1561 ctggcggcgg ccggcggtcg cctgcggcta tacaaggacc aggtcatctt cgagcgcgac
     1621 gaactgctca ccggcgccgg ccgcccgtac acggtattca ccccgtacaa gaacgcctgg
     1681 ctgaagcgcc tgagcgccgc cgaccatgca ccgttctcgg cgcaaggacg tctcgcaccg
     1741 gcgcccgaga gcctgccgac gctggccgaa ctcggcttcg cgccaaccga tctcggccaa
     1801 ctcggcatcg ttcccggcat ggccggcgcc cgccagctat gggacgagtt ccgcgccggc
     1861 cgcatccgcc gttacggcac gctgcgcgac ttccccgcac tcaagggcgt cagctacctg
     1921 tcggtgcacc tgcgcttcgg caccatttcg atccgcgaac tggtcagtgc cgcgcttgcg
     1981 gaaaaagccg atccctggct caacgaactg atctggcgcg atttctactt catgatcctc
     2041 gaccgcttcc cgcatgtcgt cgaccgcgcc ttcaagccgg aatacgacgc catccgctgg
     2101 gcagactggc cggaaggcct ggccgcctgg cagcagggac gcaccggcta cccgctggtc
     2161 gacgcggcga tgcgtcagtt gaaccacagc ggctggatgc acaatcggct gcgcatggtc
     2221 gtcgcctcct tcctgtgcaa ggacctcggc ctcgactggc ggctcggcga acgctacttc
     2281 gccgagcggc tcaacgattt cgacctggcg gccaacaacg gcggctggca gtgggcggcg
     2341 tcgagcggct gcgacgcgca accgtacttc cggatattca acccggtgac gcagtcggag
     2401 aagttcgacc ccgacggcaa attcatccgc cgctacgttc cggaactggc ccgggtgccg
     2461 gacaagtaca tccacgcccc gtggacgatg ggccggctgg agcaggaagc gctcggcgtc
     2521 gtcatcggcc gtgactatcc ggggccgatc gtcgaccacg cggcggcacg cgaaaagacg
     2581 ctggcgcgct acgctgtggt gaagaaggcg gtttgaacac ggtcgatcgc ggtagacacc
     2641 gcgatcgacc gtcgctttac gtggatttcc cgcagtagcc ggcgagcagg ccgagcagct
     2701 cgtcttcgtc gtagggcttg ccgaggtagt ggttggcacc gatctcgagc gcgtagttgc
     2761 gatgcttgtc ggcggtacgc gaggtgatca tgaccaccgg gatgtcgcgt aggcgctcgt
     2821 cggcacgcag gttgcggacg aaatcgaagc cgtccatgcg cggcatctcg atgtcggaga
     2881 ggatgacgtc gggcagcgcg tcgatcagtt gctcgagcgc atcgacgccg tccttggcga
     2941 cgaccacctg ataaccctcg cgcagcagca ggcggctggt gatcttgcgc acagtcagcg
     3001 aatcgtcgac caccatcacc gtcggcaggt gcggcgcggc aaccggcaag ggcgcagcga
     3061 ccacggccgg cgcggcgact tcagccaccg cgatcaacgg cgcccgggcg gcgagcgcca
     3121 ccgggttgag gatgagcacg acgcgaccgt cgccaagcac ggtggcaccg gcgatgccgg
     3181 caacgcgggc gagctggctg ccgatgttct tgacgacgac ttcctggttg ccttgcagtt
     3241 catcgacgag cagggcgatc cgttgcgagc cggcacgcag cagcagtatc cagtagcggc
     3301 ggcgggtttc cggcaaggct tcggcatcgc ccagcaggcg gggcaggaaa tggaaggggt
     3361 agtgattgcc ttgccagtcg gcggcgccgg cgtcgcggat ttcggcgagg cccttttcct
     3421 tgacctcgag tacctgctcg atcatcgtcg agggcacggc gtacacggtg ctgccggcgc
     3481 ggaccagcag ggtctgggtg acggccaggg tgagcggcag atagagacgg aaggtgctgc
     3541 cgtggccggc ttcggagacg atttcgatgc gtccgccgag ttcgatcacg gcattcctga
     3601 ccacgtccat gccgacgccg cgcccggcga cctgggtcac ttcggtggcg gtcgagaacc
     3661 ccggccggaa gatgaagtcg agcagggctg catcggccac ctcctgaccc ggctgcagca
     3721 ggcccatttc ctcggcgcgg gcacggatgc gctggcggtc gagaccgcgg ccgtcgtcgc
     3781 tcaggctgag gacgatttcg ttggcttcct gggccagctt cagcgtcact tcgccggttt
     3841 ccggcttgcc cgccgcggcg cgctgctcgc gcgtctcgat gccgtgggtc accgagttgc
     3901 gcaacatgtg ctcgatcggc gccatgatct tttccagcac cgagcggtcg atttcgacct
     3961 gaccgccgac gatgtcgaag ttggcgcgct tgccgacttc cttggcggtc tgccggacga
     4021 tgcggtagag acgctcggcc tggctggcga agggcagcat gcgcacgccc atcaactcct
     4081 gctgcaggcc gcggttgagg cgggcctggg cgaggatcgc cgcgttggcg tcgtcgaggt
     4141 tcttgaacag gttctgctgc accgtcgcca cgtcgttgac cgactcggcc atgaaacggg
     4201 tcagttcctg gaagcgggtg aagcggtcga gttccagtgg atcgaattct tcctggcctt
     4261 ccggggtgtg cgctacccgc gcctgcatct gggtttcggc ctgaatttca atttcgcgta
     4321 actgccggcg
//
LOCUS       DAOSDX010000121         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7169_length_4321_cov_3.160806, whole genome shotgun sequence.
ACCESSION   DAOSDX010000121 DAOSDX010000000
VERSION     DAOSDX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7169_length_4321_cov_3.160806"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLN02_03735"
     CDS             <1..596
                     /locus_tag="PLN02_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI51463.1"
                     /translation="VDLQLQARDFAAIRGPSGSGKSTLLNICGLIDRIDGGSYRLAGE
                     DISELDEAACTEIRRKRMGFIFQNYNLVPVMSVFDNVEYPLLLNNIPKAERRALVSDI
                     LDKVGLNGFAERKPDQLSGGQRQRVAIARALVKAPLLVIADEPTANLDTDTAEHIVEL
                     MKSLGHRLGTSFLIATHDDRMTRHCDKVFSMQDGVIA"
     gene            593..1810
                     /locus_tag="PLN02_03740"
     CDS             593..1810
                     /locus_tag="PLN02_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI51464.1"
                     /translation="MNHILLAWKNVLRNRRRSFVTIFIAAVGCASILIAGGFALWTYD
                     ILIEGASREFGHITVADKSFFAKEEETPMQFGMQQDNRVAEYLEKNPEVATLLPRINY
                     SGLISNGDKSLIFLGTGAELAREAAVRKYFLVVREGAIPRGEPSQLPQILLGVALARN
                     LGAKVGSQLTLIGTTAATGGINAVDVEVAALVSTGWSEVDKRLVFSNIQTAQNLLMTD
                     KISTLSVFMHHLDKVPAMLETLAAYDGEHAYMPWWEQAQYYHSVRALYNRIFGLLGMI
                     IIALVLFSVINTLAMAVVERTREIGTLRALGAHPREIIAQFVREGGIIGLAGVMIGNL
                     VAAAVIWTLPYLRLQMPPPPGRSEGYPLLVAGSPTLTLVTNALIVLLCMGAAWAVSRR
                     AASKPIVDALGHV"
     gene            1803..2603
                     /locus_tag="PLN02_03745"
     CDS             1803..2603
                     /locus_tag="PLN02_03745"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOI51465.1"
                     /translation="MSDRRPLSFLRHAGTALCLYAATAACAAGDVDALLRKADSYRLP
                     AASAQVETRVEQYRRGELDKERDYTVYIKPGRRSLILMKTAAELGQKVLMLGDNFWLF
                     VPDSQRPLRITANQKLLGEASTGDIAEMSWHEDYDGSVSEETDCPKPPEIAGIPGRVA
                     AGSCLRLQLQALRSGVTYAKIELFLEKNSKFPVKADLIMGSGKKAKEAWYFELAVQGR
                     RQVGAMLLFDHIQTTRHTVVHYRKLAAREAPDELFNPAALVRNSLAEW"
     gene            2597..3826
                     /locus_tag="PLN02_03750"
     CDS             2597..3826
                     /locus_tag="PLN02_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51466.1"
                     /translation="MVTKLLPLLAVLALGSTPALADEFNATLRITPEYHEADRGSPYF
                     AAGRPEMPGGWNSNQEIALRYRGHGFSGDATLRWETRQGEADRLRGDLNQFYFDSALG
                     DGLAWTIGKKVLTWGIGFAFRPLDVVQRENRRSVTDPMLVGIPLLAIEKFTADQAWTA
                     VWAQPGQGGDKRDRDDEALAFRWYKVADKDDLHAVARFSRTRKFEAGFGANRVIGDEW
                     SIYGATLYSHRYLNTINTLGETGGIFALTSPLAHQSGEHANKSLIGAQWTGDSGWSAL
                     LELFHDGEAYRKADWRRLDALVARQHLNAALAPATLFNANLAWSANAYTQVNVHRDNA
                     LFRVAYDNGDGFKPYLELLNTPTDGGLVTTLGGSYEGNRFKISGGVRHLGGASDSVYA
                     RAPERYMAWVQLSCPIW"
     gene            3856..>4321
                     /locus_tag="PLN02_03755"
     CDS             3856..>4321
                     /locus_tag="PLN02_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOI51467.1"
                     /translation="MATEIEIKLRLEARHVRKLLAHPLLKGMSSQKQNLLNTYYDTPE
                     LTLRQRRIALRLRKKGWEWLQTVKSAEPASGGLAIRNEWEIPATPGQFDFAHVDNAEL
                     RSLLNDTRDRLEPVFTTDFRRQAWQIACGDSLIEMALDRGRISAKERSTGICE"
BASE COUNT          835 a         1525 c         1233 g          728 t
ORIGIN      
        1 gcgtcgatct gcaactccag gcgcgcgact tcgccgccat ccgcggcccg tcgggcagtg
       61 gcaagagcac gttgctcaac atctgcggcc tgatcgaccg catcgacggc ggcagctacc
      121 ggctggccgg cgaggacatc agcgaactgg acgaggcggc gtgcaccgaa atccggcgca
      181 aacgcatggg cttcatcttc cagaactaca atctggtgcc ggtgatgagc gtattcgaca
      241 atgtcgaata ccccttgctg ctcaataaca tcccgaaggc cgaaaggcgg gcgctggttt
      301 ctgatatcct cgacaaggtt ggcctcaacg gtttcgccga gcgcaagccc gaccagcttt
      361 ccggcggcca gcgacagcgc gtcgccatcg cccgggcgct ggtcaaggcg ccgctcctgg
      421 tcatcgccga cgaacccacc gccaacctcg acaccgacac cgccgaacac atcgttgaac
      481 tgatgaaaag ccttggccac cgactcggca cttccttcct gatcgccacc cacgacgacc
      541 ggatgacccg acattgcgac aaggtattca gcatgcagga cggtgtgatc gcatgaatca
      601 catcctgctg gcgtggaaga acgtcctgcg caaccggcgt cgctccttcg tcaccatctt
      661 catcgccgcc gtcggctgcg cttcgatcct gatcgccggc ggcttcgcgc tgtggaccta
      721 cgacatcctg atcgaaggcg cctcccgcga attcggtcac atcacggtcg ccgacaagtc
      781 ctttttcgcc aaggaagaag aaactccgat gcagttcggc atgcagcagg acaatcgcgt
      841 cgcggaatat cttgaaaaga acccggaggt cgccacactg ctgccgcgga tcaactattc
      901 cggcctgatc tcgaacggcg acaaatcgct gatcttcctt ggcaccggcg ccgaactcgc
      961 ccgcgaagcg gcggtccgca agtatttcct ggtcgtccgc gaaggcgcca ttccgcgggg
     1021 cgagcccagc caactgccgc aaatcctgct cggcgtcgcc ctcgcccgca atctcggcgc
     1081 caaggtcggc tcgcaactga cgctgatcgg caccacggcc gccaccggcg gcatcaacgc
     1141 agtggacgtc gaggttgcgg cactggtctc gaccggctgg agcgaggtcg acaagcgcct
     1201 cgtcttttcc aacatccaga ccgcccagaa cctcctgatg accgacaaga tcagcaccct
     1261 gtcggtattc atgcaccacc tcgacaaggt ccctgcgatg ctcgagacgc tggccgccta
     1321 cgacggcgag catgcctaca tgccctggtg ggagcaggcc cagtactatc actcggtacg
     1381 ggccctgtac aaccgcattt tcggtctgct cggcatgatc atcatcgccc tcgtcctgtt
     1441 ttcggtgatc aataccctgg ccatggcggt ggtcgagcgg acccgcgaaa tcggcaccct
     1501 gcgcgcgctg ggcgcccacc cgcgcgaaat catcgcccag ttcgtccgcg agggcggcat
     1561 catcggcctg gccggcgtga tgatcggcaa tctggttgcc gccgccgtga tctggacctt
     1621 gccgtatctc cgcctgcaga tgccgccacc gcccggtcgc tccgaaggct acccgctgct
     1681 ggtcgccggc tcgccgaccc tgacgctggt caccaacgca ctgatcgtgc tgctgtgcat
     1741 gggggcagcc tgggcggtca gccggcgcgc cgccagcaaa cccattgtgg acgccctcgg
     1801 ccatgtctga ccgccgcccg ctttcttttc tgcgccatgc cggtaccgcg ctctgccttt
     1861 acgcagccac cgcggcctgc gccgccggcg atgtcgacgc tttactgcgc aaggccgaca
     1921 gctatcgact gccggccgct tcggcgcagg tcgaaacccg ggtcgagcaa taccggcgcg
     1981 gcgaactcga caaggaacgc gactacaccg tctacatcaa gcctggccgg cgttcgctga
     2041 tcctgatgaa aactgccgcc gaacttggcc agaaggtgct gatgctgggc gacaatttct
     2101 ggcttttcgt ccccgacagc cagcgcccgc tgcgcatcac cgccaaccag aagctgctcg
     2161 gcgaagcctc gaccggcgac atcgccgaga tgtcctggca cgaggattac gacggcagcg
     2221 tcagcgaaga aaccgactgc ccgaagcccc cggaaattgc cggcatcccc ggccgcgttg
     2281 ctgccggtag ctgcctgcgc ctccaactgc aggcgctacg cagcggcgtc acctatgcca
     2341 aaatcgagct tttcctggaa aagaacagca agtttccggt caaggccgac ctgatcatgg
     2401 gctccggcaa gaaggccaag gaagcctggt acttcgagct ggcggtgcag ggacggcgtc
     2461 aggttggcgc gatgctgctc ttcgatcata tccagaccac ccgacacacc gtcgtccatt
     2521 accgcaagct cgctgcccgc gaagccccgg acgaactttt caatcccgcc gccctagtcc
     2581 gcaactcact cgccgaatgg taaccaagct ccttcccttg ctcgccgtgc tcgcgctcgg
     2641 ctcgacaccc gccctggccg acgaattcaa cgccacgctg cgaattaccc ccgaatatca
     2701 cgaggccgac cgcggcagcc cctatttcgc agccggccgc ccggaaatgc ccggcggctg
     2761 gaactcaaac caggaaatcg ccctgcgtta ccgcggccac ggcttttccg gcgacgccac
     2821 gctgcgctgg gaaacccgcc agggagaggc tgaccgcctg cgcggtgacc tcaaccagtt
     2881 ttacttcgac agcgcactcg gcgacggtct ggcctggacc atcggcaaga aggttctgac
     2941 ctggggcatc ggctttgcct tccggccgct cgacgtcgtc cagcgtgaga accggcgtag
     3001 cgtcaccgac ccgatgctgg tcggcattcc gttgctggcc atcgaaaaat tcaccgccga
     3061 ccaggcctgg actgccgtct gggcccagcc cggccagggc ggcgacaagc gcgaccgcga
     3121 cgacgaagca ctcgccttcc gctggtacaa ggtcgctgac aaggacgacc tgcacgccgt
     3181 cgcccgcttc tcgcggacac gcaaatttga ggcaggcttc ggcgccaacc gcgtcatcgg
     3241 cgatgaatgg tcgatttacg gtgccaccct ctatagtcac cgctacctca acacgatcaa
     3301 cacgctcggc gaaaccggcg gcatcttcgc actgacctca ccactcgccc accaatcggg
     3361 cgagcatgcc aacaagagcc tgatcggcgc gcaatggacc ggtgattccg gctggagcgc
     3421 gctgctcgaa cttttccacg acggcgaagc ctaccgcaag gcggactggc gccgactcga
     3481 cgccctggtc gcccgtcaac acctgaatgc ggcgctggcc ccggcaacgc tcttcaacgc
     3541 caatctcgcc tggtcggcca atgcctacac ccaggtcaac gttcatcgcg acaacgcgct
     3601 gttccgtgtc gcctacgaca acggcgacgg cttcaagccc tatctcgaac tcctcaacac
     3661 gccgaccgac ggcggcctgg tcaccacgct gggcggcagt tacgaaggca atcgcttcaa
     3721 gatcagcggc ggtgtccgcc acctcggcgg agccagcgac tcggtctatg cccgggcacc
     3781 ggagcgctat atggcgtggg tccagttgtc ctgtcccatc tggtaaggcg ctggccgcat
     3841 tccctgagag caaccatggc gactgaaatt gaaatcaaac tgcgactgga agccagacat
     3901 gtccgcaaac tcctggccca cccgctgctc aaaggcatga gcagccagaa gcagaacctg
     3961 ctcaacacct actacgacac acctgaacta acgctgcgcc agcgccgcat cgccctgcgc
     4021 ttgcgcaaga agggctggga atggctgcag acggtgaaat ccgccgagcc cgccagtggc
     4081 ggactggcca tccgcaacga atgggagatt ccggcaacac cgggtcagtt cgacttcgcc
     4141 catgtcgaca acgctgaatt gcgcagcctg ctcaacgata cccgcgaccg tctggaacct
     4201 gtcttcacca ccgacttccg gcgccaggcc tggcaaattg cctgcggcga ctcgctgatc
     4261 gaaatggcgc tggaccgcgg caggatttcc gccaaagaac gcagtaccgg catctgcgaa
     4321 g
//
LOCUS       DAOSDX010000122         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7171_length_4320_cov_3.997186, whole genome shotgun sequence.
ACCESSION   DAOSDX010000122 DAOSDX010000000
VERSION     DAOSDX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7171_length_4320_cov_3.997186"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLN02_03760"
     CDS             <1..180
                     /locus_tag="PLN02_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51468.1"
                     /translation="GPVGMVAMFAVAGGILGHLVHLEVGHWALALLADIGIGVAVGLL
                     LVGLHHLWAKLRGQH"
     gene            279..1859
                     /gene="urtB"
                     /locus_tag="PLN02_03765"
     CDS             279..1859
                     /gene="urtB"
                     /locus_tag="PLN02_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HOI51469.1"
                     /translation="MKKRLLTFLLWATSLGALAAPDPALVRQLAADDNSDKIAAIQQL
                     TAAAEPGTAKLLQAMADDSLYVAGDKLYVYDGEKTVDALNGETAELPDGAETITVNNR
                     LRGELANALAALKLFDADRAVRLAAARELQSQVGAELAGVLAKARDQEQDAEIKPLLA
                     LAHAQATLQDADPAVRLAAVNTLAEAATPAVKSLLLPLTEASGESDAKVRAAASAALK
                     SIERSLAWMDTLAGIFSGLSLGSILLLAALGLAITYGVMGIINMAHGELLMIGAYSAY
                     AMQSLFRAYFPEQVDWYLPAAIPVAFFAAAAVGVLMERTVIRWLYGRTLETLLATWGL
                     SLILIQAARVLFGAQNVEVANPSWMSGGVELLPNLILPWNRVIIIGFALFVLFLVWVL
                     MNKTRLGMFVRAVTQNRAMAGCVGVPTARIDTMAFALGAGIAGLGGVALSQISNVGPD
                     MGQGYIVDSFMVVVVGGVGQLAGAVWAALGLGIFSKLLEGWAGAVIAKIMILVAIIIF
                     IQKRPQGIFALKGRFADQ"
     gene            1870..3021
                     /gene="urtC"
                     /locus_tag="PLN02_03770"
     CDS             1870..3021
                     /gene="urtC"
                     /locus_tag="PLN02_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HOI51470.1"
                     /translation="MSATSSVLPLPAGRVPLTLRVFAALDRKAWTALAVFLALALVVV
                     PVLNLALPPESVFHVSAYAITLIGKIMCYALVAVAMDLIWGYGGILSLGHGLFFALGG
                     YAFGMYLMRQIGRDGSYGADLPDFMVFLDWKELPWFWLGSDSFGWAAFLVVALPAVVA
                     FVFGYFAFRSRIKGVYFSIITQALTYAAMLLFFRNETGFGGNNGFTDFKRVLDVPITA
                     PETRLVLFGLTAIALAATLVFAAWLVKSKFGRVLTAIRDAESRVMFIGYNPLWYKLTI
                     WVISAVLCGIAGALYVPQVGIINPSEMSAANSIEIAIWVAVGGRGTLIGPVIGAFTVN
                     LAKSWFTVSFPEYWLFFLGLLFIVVTLLLPQGLVGLWKKVISKKQGAQA"
     gene            3018..3848
                     /gene="urtD"
                     /locus_tag="PLN02_03775"
     CDS             3018..3848
                     /gene="urtD"
                     /locus_tag="PLN02_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HOI51471.1"
                     /translation="MNAQAALIDQPDGGDSATQLGHLVTGELDLSHGVALYLEDITVS
                     FDGFKALNNLTLAINAGELRCIIGPNGAGKTTMMDVITGKTRPDSGKAFFGQSIDLTR
                     LTEPEIAHVGIGRKFQKPTIFEQHTAFENLELAMKTDKRVWKSLLAWLSGDDRDRIAD
                     TLKLIRLDGEADRPAGLLSHGQKQWLEIGMLLMQEPKLLLLDEPVAGMTDDETMRTAE
                     LFVSLAGKHSLVVVEHDMAFVEKLGGKVTVLHEGSVLAEGDLATVQNDQRVIEVYLGR
                     "
BASE COUNT          686 a         1508 c         1354 g          772 t
ORIGIN      
        1 ggcccggtcg gcatggtcgc catgttcgcc gtcgccggcg gcatcctcgg ccacctcgtc
       61 catctcgaag tcggccattg ggcgctcgcc ctgctcgccg acatcggcat cggcgtcgcc
      121 gtcggcctgc tgctggtcgg cctgcatcac ctgtgggcca agctgcgcgg ccagcactga
      181 aaccgggggc aagtcccccc tacgcaattc atcgtattcc ccgcgccggg gccgctccgt
      241 aatctgtcgc tgcactgcac aaattgcgga cggacccgat gaaaaaacgc ttactgacct
      301 tcctgctctg ggccaccagc ctgggcgccc tggccgcccc cgacccggcg ctggtccgcc
      361 aactggcggc cgacgacaac tccgacaaga tcgccgccat ccagcaactg accgccgccg
      421 ccgagcccgg cacggcgaag ctgctgcagg cgatggcgga cgacagtctc tatgtcgccg
      481 gcgacaaact ctatgtctac gacggcgaga agacggtcga cgcactgaac ggcgaaaccg
      541 cggaactgcc cgacggcgcc gagacgatca ccgtcaataa ccgcctgcgt ggcgaactgg
      601 ccaacgcact ggccgcgctc aaactgttcg atgccgaccg cgccgtccgc ctggccgccg
      661 cccgcgaact gcagagtcag gtcggcgccg agctggccgg cgtgctggcc aaggcgcgcg
      721 accaggaaca ggacgccgag atcaagccgc tgcttgccct ggcgcacgct caggcgacgc
      781 tgcaggatgc cgacccggcg gttcgcctgg ccgccgtcaa caccctggcc gaggctgcca
      841 cgccggcggt caagagcctg ctgctaccgc tcaccgaggc cagcggcgaa agcgacgcca
      901 aggtgcgagc cgcggcgagc gccgcgctga agagcatcga gcgcagcctg gcctggatgg
      961 acacgctggc cggcatcttc tccggcctgt cgctcggctc catcctgctg ctggcggcgc
     1021 tcggcctggc catcacctac ggcgtcatgg gcatcatcaa catggcgcac ggcgaactgc
     1081 tgatgatcgg cgcctactcg gcctacgcga tgcagagcct gttccgcgcc tatttcccgg
     1141 aacaggtcga ctggtacctg ccggcggcga ttccggtcgc cttctttgcc gccgccgccg
     1201 tcggcgtgct gatggagcgc acggtgatcc gctggctgta cggacgcacg ctggaaaccc
     1261 tgcttgccac ctggggcctg tcgctgatcc tgatccaggc ggcgcgcgtg ctgttcggcg
     1321 cgcagaacgt cgaagtcgcc aacccgagct ggatgtcggg cggcgtcgaa ctcctgccca
     1381 acctgatcct gccctggaac cgggtgatca tcatcggctt cgcgctgttc gtgctgttcc
     1441 tggtctgggt gctgatgaac aagacccggc tcggcatgtt cgtccgcgcc gtcacccaga
     1501 accgggcgat ggccggctgc gtcggcgtgc cgacggcgcg tatcgacacc atggccttcg
     1561 ccctcggcgc cggcatcgcc gggctgggtg gcgtggccct gtcgcagatt tccaacgtcg
     1621 gcccagacat gggccagggc tacatcgtcg actccttcat ggtcgtcgtc gtcggcggcg
     1681 tcgggcaact ggccggcgcg gtctgggccg ccctcggcct gggcatcttc tccaagctgc
     1741 tcgaaggctg ggccggcgcg gtgatcgcca agatcatgat cctcgtcgcc atcatcatct
     1801 tcatccagaa gcgtccgcag ggcattttcg ccctcaaggg ccgctttgcc gaccagtaag
     1861 gagcgacgca tgtctgccac ctcttccgtg cttcccctgc ccgccggccg cgtgccgctg
     1921 accctgcgcg tctttgccgc gctcgaccgc aaggcctgga ccgcgctcgc cgtcttcctc
     1981 gccctggcgc tggtcgtcgt cccggtgctc aacctggcgc tgccgccgga gagcgttttc
     2041 cacgtctcgg cctacgcgat cacgctgatc ggcaagatca tgtgctacgc gctggtcgcc
     2101 gtcgccatgg atctgatctg gggttacggc ggcatcctct cgctcggcca cggcctcttc
     2161 ttcgcgctcg gcggctacgc cttcgggatg tacctgatgc gccagatcgg ccgcgacggc
     2221 agctacggcg ccgacctgcc cgacttcatg gtcttcctcg actggaagga actgccctgg
     2281 ttctggctcg gcagcgacag cttcggctgg gccgccttcc tggtcgtcgc gctgccggcc
     2341 gtggtcgcct tcgtcttcgg ctacttcgcc ttccgctcgc ggatcaaggg cgtctatttc
     2401 tcgatcatca cccaggcgct gacctacgcc gcgatgctgc tcttcttccg caacgaaacc
     2461 ggtttcggcg gcaacaacgg cttcaccgac ttcaagcgcg tgctcgacgt gccgatcacc
     2521 gcgccggaaa cccgcttggt gctgttcggc ctgaccgcca tcgccctcgc cgcaacgctg
     2581 gtcttcgccg cctggctggt caagtcgaag ttcggccgcg tgctcaccgc gatccgcgac
     2641 gccgaatccc gcgtcatgtt catcggctac aacccgctct ggtacaagct gaccatctgg
     2701 gtgatttccg ccgtgctgtg cggcatcgcc ggcgcgctgt acgtgccgca ggtcggcatc
     2761 atcaacccgt cggaaatgag cgccgccaac tcgatcgaaa tcgccatctg ggtcgccgtc
     2821 ggcgggcgtg gcacgctgat cggcccggtc atcggcgcct tcacggtcaa cctggcaaaa
     2881 agctggttca ccgtcagctt cccggaatac tggctgttct tcctcggcct gctgttcatc
     2941 gtcgtcaccc tgctgctgcc gcaaggcctg gtcggactgt ggaagaaagt catcagcaag
     3001 aaacaaggag cccaggcatg aacgcccaag cagccctcat cgatcaaccg gacggcggcg
     3061 acagcgccac ccagctcggc cacctggtca ccggcgaact cgacctgtcg cacggcgtcg
     3121 cgctctatct cgaagacatc accgtcagct tcgacggctt caaggcgctg aacaacctga
     3181 cgctcgccat caacgccggc gaactgcgct gcatcatcgg ccccaacggc gccggcaaga
     3241 cgacgatgat ggacgtcatc accggcaaga cgcgcccgga ttccggcaag gccttcttcg
     3301 gccagagcat cgacctgacc cgcctgaccg aaccggaaat cgcccacgtc ggcatcggcc
     3361 gcaagttcca gaaaccgacc atcttcgaac agcacaccgc tttcgagaac ctcgaactgg
     3421 cgatgaagac cgacaagcgc gtgtggaaga gcctgctcgc ctggctgagc ggcgacgacc
     3481 gcgaccgcat cgccgacacc ctgaaactga tccgcctcga cggcgaagcc gaccgcccgg
     3541 ccggcctcct ctcgcacggc cagaagcagt ggctggaaat cggcatgctg ctgatgcagg
     3601 aaccgaagct gctgctgctc gacgaaccgg tcgccgggat gaccgacgac gaaaccatgc
     3661 gcacggcgga gctgttcgtt tccctggcgg ggaagcattc gctggtcgtc gtcgaacacg
     3721 acatggcgtt tgtcgagaag ctgggcggca aggtcaccgt gctgcacgaa gggtcggtgc
     3781 tggcggaggg cgatctggcg acggtgcaga atgatcagcg ggtgattgag gtttatcttg
     3841 ggcgctgagg ctttttcttt cgcgctgcgc gggtgggcgg aatcggttgc tgaggcgccg
     3901 atttccgcgc cggacgtgcg ggtgtgttcg gttgctgaag cgttgggttt ccttatgctt
     3961 aggcgagcgg ctttggtttc tgaaccgctg tattccgccg ttgaccggcg ggcgtacttt
     4021 cttttttgtg gaaaaaagaa agtagccaaa gaaaaagcca cccccaggtc ggcgccgggc
     4081 tgcgcccggt cccttgcgct actcggcgcg ccgggcggct gcggaactcg gccctgcggg
     4141 cctcagacag tcctcgccga cttcccccgg cacacctgcg ttgctcagcg cctctcaggg
     4201 gggccccgaa aaccagcggt atgcgacggt ttgcggttga aagagcgttg cactttcagc
     4261 agcgatcggt ttgccgggcc ccttgggagg cgccgagcaa cgcaggcgtt ggcggataaa
//
LOCUS       DAOSDX010000123         4319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7173_length_4319_cov_3.549015, whole genome shotgun sequence.
ACCESSION   DAOSDX010000123 DAOSDX010000000
VERSION     DAOSDX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7173_length_4319_cov_3.549015"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..719
                     /locus_tag="PLN02_03780"
     CDS             75..719
                     /locus_tag="PLN02_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HOI51472.1"
                     /translation="MIYPNLNPEKALIWRIVHRDNLPWILDNGLHCGNSALKAPAWVN
                     IGNPELIDKRANHPVPLPPGGLLNDYVPFYFTPFSPMLRNIHTGWGGIPKRPNEEIVI
                     LVSSLHHIAGLGLQFLFTDSHAYYQWANFYADLADLDKIDWPLLQRRDFKRDPEDPAK
                     FERYQAEALVHQHLPVCGLRGIVCYTEALKQRIEPEIQARNLNLPVHARTGWYF"
     gene            719..1795
                     /locus_tag="PLN02_03785"
     CDS             719..1795
                     /locus_tag="PLN02_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOI51473.1"
                     /translation="MITFTQGNLLAARAEALVNTVNTVGVMGKGIALMFKERFDENFR
                     RYAAACKAREVQTGKMFVTQVHELDGPRWIVNFPTKQHWRTPSRMEWIVEGLQDLKRF
                     LIEQQVKSIAIPPLGAGNGGLEWAEVREQIEQSLGDLDIDILVFEPTRQYQNVAKRSG
                     VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIERHNPGDNPLNLQFAPH
                     KYGPYANRLDHLLNNLDGSYLHSEKRISDADPLDVIWFDDERKAFVQTYLKSEAKAYT
                     QALESTAALIDGFESPFGMELLATVDWLLAREGVAPNVPALRAGLRRWRGGPDAAARK
                     ERLFDDRALGIALNRLTQATTVPA"
     gene            complement(1830..2438)
                     /locus_tag="PLN02_03790"
     CDS             complement(1830..2438)
                     /locus_tag="PLN02_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51474.1"
                     /translation="MSFPEFYEHVPRLRLRDPLAGLLGASADGILDYGYADAVRLAGH
                     SCPTVAAAYWLTWRALALLYGTALPVRGEIRVSFRQPRDEGVTGVTAAVVGLLTGAAG
                     SEGFKGLGGRYARRGLLNFAATIPLELRFERLDTGARVDAAAYPQQLPADPALSDLLA
                     AVTFGTADAAARQRFGEIWQDRVARLLTEHRDDPAVFDLRVG"
     gene            complement(2459..3202)
                     /locus_tag="PLN02_03795"
     CDS             complement(2459..3202)
                     /locus_tag="PLN02_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI51475.1"
                     /translation="MMTCIPRPDFDDHAACDPALLEVAQILAGTPLFSGLRPDDLLRL
                     AAGCRREHYDKGNILFHKGDPCHGFYLALEGQIKLAFISQDGNQKVVEIIRPGQTFGE
                     AVMFMERPYMLMAQALTDCRLLHIAKDAVFAELARDPQMCRKLIAGLSMRLHHLIGDL
                     EAHSLRSGRERIIGYLLREEEMAGPVATAGAVTIRLPTHKGTIASRLNLTQEHFSRIL
                     HELVDEGLVVVQGRLIHVPDIGRLRAGLV"
     gene            complement(3207..>4319)
                     /locus_tag="PLN02_03800"
     CDS             complement(3207..>4319)
                     /locus_tag="PLN02_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HOI51476.1"
                     /translation="PQRDGIMMYVRAESLLSVAEFIRVNRGVDDDPDANAAEVAADLD
                     RLSLTEAAPGERVASRVRFDLDLPSAAEDDIVLGPGLRLPEWDYRKSRLLDDHVCLQE
                     LASRHAVPTPLPPRLRRTARRLHQQFAALQPGRRWLKGQPDGSEADLDAVVRACTDRA
                     CGRHPGEQLYLTLERRERDLACLVLADLSLSTDAWVSSEARVIDVIRDALLLFGEALA
                     ATGDAFALCGFSSVRRQQVRFHRLKDFGDPFDDRIRGRVQAIRPGYYTRLGAAIRRAS
                     QLLDAQAASRRILLILSDGKPNDLDLYDSRYGIEDTRAAVLEARRLGLLPFCLTIDRE
                     GAGYLPHLFGPQGYAVLRKPEELPLHLPLFYAQLTR"
BASE COUNT          764 a         1335 c         1533 g          687 t
ORIGIN      
        1 tcattcgcca tgaagcgttg catagtcggt attcgcccct caggtacgca cgatgccacg
       61 gcgattgagc cttgatgatt taccccaacc ttaatcccga gaaggcgctg atctggcgca
      121 ttgtccatcg cgacaacctg ccgtggattc tggacaacgg cctccactgc ggcaacagtg
      181 cgctgaaggc gccagcctgg gtaaatatcg gcaacccgga actgatcgac aagcgggcca
      241 atcacccggt ccctttgccg cccggaggtt tgttgaacga ctatgtgccg ttctacttca
      301 cgccgttttc gcccatgctg cgcaacatcc acaccggttg gggcggtata ccgaaacggc
      361 cgaatgagga aatcgtgatt ctggtctcca gcctgcatca cattgccgga ctgggcttgc
      421 agtttttatt taccgatagc cacgcctact accagtgggc aaacttttac gctgaccttg
      481 ccgatctgga caagatcgac tggcccctgc tgcaaaggcg ggacttcaag cgggacccgg
      541 aagaccccgc caaattcgag cgctatcagg ctgaagcgct ggttcatcag cacttgccgg
      601 tgtgcggact tcgcggcatc gtttgctaca cggaagcatt gaaacaacgt atcgaaccgg
      661 aaatacaggc cagaaatctg aacctgccgg ttcatgccag aacagggtgg tacttctgat
      721 gatcacattc actcaaggca atctgctggc ggcccgcgcg gaggcgctgg tcaacacggt
      781 caacaccgtc ggggtgatgg gcaagggcat cgcgctgatg ttcaaggagc gtttcgacga
      841 gaacttccgc cgttacgctg cagcctgcaa agccagggaa gtgcaaaccg gcaagatgtt
      901 cgtaacgcag gtacacgaat tggatggccc gcggtggatc gtcaacttcc ccaccaagca
      961 gcactggcgg acaccctcgc gcatggagtg gattgttgaa ggactgcagg acttgaagcg
     1021 cttcttgatt gagcagcagg tcaagtccat tgccattccg ccactgggtg cgggcaatgg
     1081 cggcctggaa tgggccgagg tacgcgaaca gattgagcag tcactgggcg acctagatat
     1141 cgacatcctg gtgttcgagc ccaccagaca gtatcagaac gtagccaaac gcagcggcgt
     1201 cgagaagctc accccggccc gcgcactgat tgccgaactg gtgcgccgct actgggtgct
     1261 gggcatggag tgcagcctgc tggagataca gaagctggcg tggttcctgg agcgctccat
     1321 cgagcgtcac aaccccggcg acaacccgct gaacctgcag ttcgcgccgc acaaatacgg
     1381 cccctacgcc aaccgacttg accatctgct gaacaatctc gacggcagct acctgcactc
     1441 cgaaaaacgt atcagcgatg ccgatccgct ggacgtgatc tggttcgacg atgagcgcaa
     1501 ggctttcgta cagacctatc tcaagagcga ggcaaaagcc tacacgcaag cgctggaatc
     1561 caccgccgca ctgatcgatg gtttcgaatc cccctttggc atggaactgc tggctaccgt
     1621 ggactggctg cttgcccggg aaggcgtcgc ccccaacgtt cctgccttgc gcgctgggct
     1681 gcgtcgctgg cgcggcggtc ccgatgcggc ggcgcgcaag gaacgcctgt tcgacgaccg
     1741 ggcgctgggc attgccctga atcggctgac acaagcgacc accgtgccgg cttgagggcc
     1801 ggactcgcca aaacccggaa ctcgccccct cacccaaccc gcaaatcaaa caccgccgga
     1861 tcatcgcggt gttctgtaag caggcgggcg acgcggtcct gccagatttc tccgaagcgc
     1921 tgccgggcgg cggcgtcggc ggtgccgaag gtgacggcgg cgagcaggtc ggagagcgcc
     1981 ggatcggcgg gcagctgttg cgggtaggcg gcggcgtcga cgcgggcgcc ggtgtcgagg
     2041 cgttcgaagc gcagctcaag cgggatggtc gccgcgaagt tgagcaggcc gcgccgggca
     2101 tagcggccgc ccaggccctt gaagccttcg ctgccggcgg cgccggtgag gaggccgacg
     2161 acggcggcgg tgacgccggt gacgccttcg tcgcgcggct ggcggaagct gacgcggatt
     2221 tcgccgcgca ccggcagcgc cgtgccgtag agcagcgcca gcgcccgcca ggtcagccag
     2281 taggcggcgg cgacggtggg gcaggaatgg ccggccaggc ggacggcgtc ggcgtagccg
     2341 tagtcgagga tgccgtcggc gctggcgccg agcagcccgg ccagcggatc gcgcaggcgc
     2401 aggcggggaa cgtgttcgta gaattcaggg aaggacatgg cgggttcctt ggacgaagtc
     2461 agacgaggcc ggcgcgcagg cggccgatgt cggggacgtg gatgaggcgg ccctggacga
     2521 cgaccaggcc ttcatcgacc agttcgtgga ggatgcgcga gaagtgttcc tgggtcaggt
     2581 tgaggcgcga ggcgatggtg cccttgtgcg tcggcaggcg gatcgtcacc gcgccggcgg
     2641 tggcgaccgg gccggccatt tcctcctcgc gcagcaggta gccgatgatg cgctcgcgcc
     2701 cggagcgcag cgagtgcgct tcgaggtcgc cgatcaggtg atgcaggcgc atcgacaggc
     2761 cggcgatcag cttgcggcac atctgcgggt cgcgcgccag ttcggcgaac accgcgtcct
     2821 tggcgatgtg cagcaggcgg cagtcggtca gcgcctgcgc catcagcatg taggggcgct
     2881 ccatgaacat caccgcctcg ccgaaggtct ggccggggcg gatgatctcg accaccttct
     2941 ggttgccgtc ctgggagatg aaggcgagct tgatctggcc ttccagcgcc aggtagaagc
     3001 cgtggcaggg atcgcccttg tggaacagga tgttgccctt gtcgtagtgc tcgcgccggc
     3061 agccggcggc gaggcgcagc aggtcgtcgg ggcgcagccc ggaaaacagc ggcgtgccgg
     3121 ccaggatttg cgccacttcg agcagcgccg ggtcgcaggc ggcatggtcg tcgaaatcgg
     3181 gccggggaat gcaggtcatc atggcctcac ctcgtcagtt gggcgtagaa cagcggaaga
     3241 tggagcggca gctcctccgg cttgcgcagc acggcgtagc cctgcggccc gaacaggtgg
     3301 ggcaggtagc cggcgccttc gcggtcgatc gtcaggcaga aaggcagcag gcccaggcgg
     3361 cgcgcttcga gcaccgccgc ccgggtgtcc tcgatgccgt agcggctgtc gtagaggtcg
     3421 aggtcgttgg gcttgccgtc ggacaggatg agcaggatgc gccggctcgc cgcctgcgcg
     3481 tcgagcagtt ggctggcgcg gcggatggcg gcgccgagcc gggtgtagta gccggggcgg
     3541 atggcctgga cgcggccgcg gatgcggtcg tcgaagggat cgccgaaatc cttcaggcga
     3601 tggaaacgga cctgctgccg gcgcaccgag gaaaagccgc acagcgcgaa ggcgtcgccg
     3661 gtggcggcga gcgcctcgcc gaacagcagc agggcgtcgc ggatgacgtc gatgacgcgc
     3721 gcctcgctcg acacccaggc gtcggtggac agcgagaggt cggcgagcac caggcaggcg
     3781 aggtcgcgtt cgcgccgttc cagcgtcagg tagagctgct cgccgggatg gcggccgcag
     3841 gcgcggtcgg tgcaggcgcg gacgacggcg tcgaggtcgg cctcgctgcc gtcgggctgc
     3901 cccttgagcc agcgccggcc gggctgcagg gcggcgaact gctggtgcag gcggcgcgcg
     3961 gtgcggcgca ggcgcggcgg cagcggcgtc ggcacggcat ggcgggaggc cagctcctgc
     4021 aggcagacgt ggtcgtcgag caggcggctc ttgcggtaat cccattccgg caggcgcagg
     4081 ccggggccga gcacgatgtc gtcctcggcc gccgacggca ggtcgaggtc gaagcgcacc
     4141 cgcgaggcga cccgctcgcc gggcgccgcc tcggtcagcg acaggcggtc gaggtcggcg
     4201 gcgacctcgg cggcgttggc atcgggatcg tcgtcgacgc cgcgattgac gcggatgaat
     4261 tcggccaccg agagcaggct ttcggcgcgg acgtacatca tgatgccgtc gcgctgcgg
//
LOCUS       DAOSDX010000124         4309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7194_length_4309_cov_3.053126, whole genome shotgun sequence.
ACCESSION   DAOSDX010000124 DAOSDX010000000
VERSION     DAOSDX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7194_length_4309_cov_3.053126"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..2082)
                     /locus_tag="PLN02_03805"
     CDS             complement(40..2082)
                     /locus_tag="PLN02_03805"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI51477.1"
                     /translation="MRGAFSSRPIAQQLIVALVGALLLVFTAMTAIVQWRADGAALSV
                     AEHNLTHEAKLMAGTLDALFEAVRDRGNRQSDFFMRNLNGTLQPGSELVRTGDVDLPV
                     VRLGDEVLNANDRVLKAFKALTGDEAALLFLRDGKVYRLSTLLRDKDGKPMHGVPVKA
                     DDPVTLSLLADKDYQGLAVRGGKYYFSTVRLLRDAAGKAWGAVSVRIALEEELKRIRA
                     QFGSIVAGNTGYVYIVRPVGDKGDGEFVLHPKFQEKLLSEVDVPATAKNAVSEILARK
                     NGAYRYLMPDGNGGEREKIVVAATSEAWGWTVATGSWLDEYLKESHALRDLVIVISIV
                     AALVLAVITFWLVNSRLRGLSQLVGEVSRISSGDLRVSVRDADPASRNEVHVIASAFN
                     EMAASMRRLVSGAAQTSGQVGDAAHALQEAAQRALESAGQASQSASGIAASVEQLSVS
                     ISQVADNANHAAEISENSKAVTASGRQVVTRTMQELERVAGDISESAVLIESLGERSK
                     QISSVVGVIREIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRKLAERTAMSTQEI
                     STTVTAILQETTNAVERMQAVSGNMSSSVGLAREAGEALNSIDARVQETVGVVMHIAE
                     GTREQSAASQEIARLVERIAQAAEGTNERALGNRQRAQELEHLADELQAQLSRFTT"
     gene            2175..2249
                     /locus_tag="PLN02_03810"
     tRNA            2175..2249
                     /locus_tag="PLN02_03810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2208..2210,aa:Arg,seq:cct)
     gene            2267..3040
                     /locus_tag="PLN02_03815"
     CDS             2267..3040
                     /locus_tag="PLN02_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOI51478.1"
                     /translation="MKTRPTPPRHAIDISEEDGVRCLHFGSDWIQGAMRVARPWSLLL
                     EYTRDMMAGLLLREAPRWPRNVLLIGLGAGSLAKFLYRNFPDCRITVVEINPQVEFVA
                     RQYFRLPDDPLRLHVEIACGADYLEGTKRRFDLILSDGFGADGRPGMLDTPAFYQACR
                     SHLNDDGLFCVNLLREKGFARSVERIAEAFSGQILLLPECTSGNTIVFGRDAQAVQAS
                     FVDLETRAQTLRQQTRLDLRPLLRRLRQAAADGAETLAF"
     gene            3163..3432
                     /locus_tag="PLN02_03820"
     CDS             3163..3432
                     /locus_tag="PLN02_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51479.1"
                     /translation="MLSMQDVLDYCDLDRGEIEAIAEHEHLPLAIAAELSEVLLGTPE
                     GVCQLHAMILENMQQALAAGDNQRVLELMETYEHLKRTHPLPSMH"
     gene            complement(3398..3784)
                     /locus_tag="PLN02_03825"
     CDS             complement(3398..3784)
                     /locus_tag="PLN02_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOI51480.1"
                     /translation="MSQAIILFAHGARDPEWAAPMRRVRAAILANAPQLAVELAFLEF
                     MAPDLPACVAELAGRGARRILILPMFIAQGGHLKREVPEIVDRLRATWPAIEFSLGRA
                     IGEEESVVQAMAAAALNASTAAGVSA"
     gene            complement(3849..>4309)
                     /locus_tag="PLN02_03830"
     CDS             complement(3849..>4309)
                     /locus_tag="PLN02_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOI51481.1"
                     /translation="IGFAGGLTPGRALAHPASRAAALNALAIAAYTVVDGIGARQSGN
                     PLAYACMLFVGEPLLILVWQYRLRAPALRSYFRSHWRLGLLGAVCSTSAYATILWAMT
                     QAPVAMVAALRESSVVFAVLIGSLWFKEGRLRPGLLAGAGVVLGIFLLRS"
BASE COUNT          744 a         1469 c         1367 g          729 t
ORIGIN      
        1 aaggcgccga tgtaatggga atggcaggat tctaatcgtt caggtggtga aacgggaaag
       61 ttgtgcctgc agctcgtcgg ccaggtgttc cagttcctgc gcccgctgcc ggttgcccaa
      121 cgcgcgttcg ttggtgccct cggcggcctg ggcaatgcgt tcgacgaggc gggcgatttc
      181 ctggctggcg gcgctctgct cgcgggtgcc ttcggcgatg tgcatgacca cgccgaccgt
      241 ttcctgaaca cgggcatcga tgctgttgag cgcttcgccg gcttcgcgcg ccaggccgac
      301 gctgctgctc atgttgccac tgaccgcctg catgcgttcg acggcattcg ttgtctcctg
      361 caggatcgcc gtcaccgtgg tcgagatttc ctgcgtcgac atcgcggtgc gttcggccag
      421 cttgcgcacc tcgtcggcga cgacggcgaa accgcggccc tgctcgccgg cgcgggctgc
      481 ctcaatggct gcgttgaggg caagcaggtt ggtctgctcg gcaatctcgc ggatgacgcc
      541 gacaacgctg gaaatctgct tcgagcgctc gcccagcgac tcgatcagca ctgccgattc
      601 gctgatgtcg ccggccaccc gctccagctc ctgcatggtc cgggtgacga cctggcggcc
      661 gctggcggtg accgccttcg agttttcgga aatctccgcc gcatggttgg cattgtcggc
      721 gacctgcgag atgctgaccg atagctgctc gaccgaggcg gcgatgccgg aggccgactg
      781 cgacgcctgg ccggcactct ccagcgcccg ttgtgccgcc tcctgcaagg catgcgcggc
      841 gtcgccgacc tgccccgagg tctgcgccgc gccgctgacc agccggcgca tgctggcggc
      901 catctcgttg aaggcgctgg cgatcacatg cacctcgttg cgactggccg ggtcggcgtc
      961 gcgcacgctg acccggaggt cgcccgagct gatccgggaa acctcgccga ccagttgcga
     1021 caagccgcgc aagcgggaat tgacgagcca gaaagtgatc accgcgagca ccagagcggc
     1081 gacgatgctg atcacgatga ccagatcgcg cagggcatgg ctttccttga ggtactcgtc
     1141 gagccagctg ccggtggcga ccgtccaccc ccaggcttcc gaggtcgcgg caacgacgat
     1201 cttctcgcgt tcgccgccgt tgccgtcagg catcaggtag cgataggcgc cattcttgcg
     1261 ggcgaggatt tcgctgactg catttttcgc ggtcgccggc acatcgactt cggagagcag
     1321 cttttcctgg aatttcggat gcaggacgaa ctcaccgtcg cccttgtcgc cgaccggccg
     1381 cacgatatag acgtaaccgg tattaccggc gacgatgctg ccgaactggg cgcggatgcg
     1441 cttgagttcc tcctccagcg cgatgcgcac ggaaaccgcc ccccaggcct tgccggcagc
     1501 gtcgcgcagc aggcgcacgg tgctgaagta gtacttgccg ccgcgcaccg ccagcccctg
     1561 gtaatccttg tcggcgagca gcgacagcgt caccgggtcg tcggccttga ccggcacgcc
     1621 gtgcatcggc ttgccgtcct tgtcgcgcaa caacgtggag aggcgataaa ccttgccgtc
     1681 gcgcaggaac aacaaggctg cctcgtctcc ggtcagcgcc ttgaaggctt tgaggacacg
     1741 atcgttggcg ttgagcacct cgtcgccgag acggaccact ggcagatcaa cgtcaccggt
     1801 acgcaccagt tcgctacccg gctgcaacgt gccgttgagg ttgcgcatga agaaatcgga
     1861 ctgccggtta ccgcggtcgc gcaccgcctc gaacagcgca tcgagagttc ccgccatcag
     1921 tttcgcttca tgcgtcagat tgtgctcggc gacgctcagc gcggcaccat cggcgcgcca
     1981 ctggacgatc gcggtcatcg ccgtgaatac cagcagcaag gcgccgacca gcgcgacgat
     2041 caattgctgg gcgataggcc gactggaaaa agcacccctc atttttgtct ccatttttgt
     2101 gatttgtcgt cagctcaatc tatcacaagg gtcggccagc cggtacaatg cgcccctgcc
     2161 cgccaaaccg gcatgcctcc atagttaaat ggatataaca cgcccctcct aagggcgaat
     2221 tggtggttcg attccacctg ggggcgccac cccacccgcg atcccgatga aaacccgccc
     2281 gacaccgccc cgccacgcca tcgacatcag cgaggaggat ggggtgcgct gtctgcattt
     2341 cggctccgac tggatccagg gcgccatgcg cgtcgcccgg ccgtggtcgc tgctgctcga
     2401 atacacccgc gacatgatgg ccggcctgct cctgcgcgag gccccgcgct ggccgcgcaa
     2461 tgtcctgctg atcggcctcg gcgccggatc gctggcgaag ttcctgtacc gcaactttcc
     2521 cgattgccgg atcaccgtcg tcgagatcaa cccgcaggtc gaattcgtcg cccgccagta
     2581 cttccgcctg cccgacgatc cgttgcgcct gcatgtcgag atcgcctgcg gcgccgatta
     2641 cctggaaggc acgaaacgcc gcttcgacct catcctctcc gacggcttcg gcgccgacgg
     2701 tcgccccggc atgcttgaca ccccggcctt ctaccaggcc tgtcgcagcc acctcaacga
     2761 cgacggcctg ttctgcgtca acctgctgcg cgagaagggt ttcgcgcgca gcgtcgagcg
     2821 cattgccgag gcgttttccg ggcaaatcct gctgctgccg gaatgcacca gcggcaatac
     2881 catcgttttc ggccgcgacg cgcaagcggt ccaggcttcc ttcgtcgacc tggaaacccg
     2941 ggcccagacg ctgcgccaac agacccggct cgacctgcgc ccgttgctgc ggcgcctgcg
     3001 ccaggcggca gcggacggag cggaaacgct ggccttctga acggctttgc ccggccgcga
     3061 taagctgcta taaatggagg aagcggtcct ccaccgacaa cgatgacaag gcggacagac
     3121 agaaaccaaa ggcgttcgcc gcttctgcca ggaggagatt ccatgttgtc catgcaagac
     3181 gttctcgatt attgcgacct cgatcgcggc gaaatcgaag ccatcgccga acacgagcac
     3241 ctgccgctag ccattgccgc ggagttaagc gaagtgctgc tcggtacgcc ggaaggcgtc
     3301 tgccagttgc acgcgatgat cctcgaaaac atgcagcagg cactggccgc cggcgacaac
     3361 cagcgggtgc tcgagttgat ggaaacctac gaacacctca agcggacaca cccgctgccg
     3421 tcgatgcatt gagcgccgca gcggccatcg cctgaacgac gctttcctcc tcgccgatcg
     3481 ccctgcccag cgaaaattcg atcgccggcc aggtggcgcg caggcggtcg acgatttccg
     3541 ggacttcgcg cttcaggtgc ccgccctggg cgatgaacat cggcaggatc aggatgcgcc
     3601 gcgcgccgcg cccggccagt tcggcgacgc aggccggcag gtcgggggcc atgaattcga
     3661 ggaaggcgag ttcgaccgcc agttgcggcg cattggccag gatggcggcg cgcacccggc
     3721 gcatcggcgc cgcccattcg ggatcgcggg cgccgtgggc gaagaggatg atggcttggg
     3781 acatgggctt tcctgaggcg ggctgagggg catcttaccc gtcacagacc gccagcgccg
     3841 cccgaggttc agctgcgcag caggaaaatg cccaacacca cgccggcgcc ggccagcagc
     3901 ccggggcgca gccgcccttc cttgaaccac aggctgccga tcaacaccgc aaacaccacc
     3961 gaactttcac gcagcgccgc gaccatcgcc accggcgcct gcgtcatcgc ccacaggatg
     4021 gtcgcgtagg cactggtcga gcagaccgcc ccgagcaagc cgagacgcca gtggctgcgg
     4081 aaatagctgc gcaaggccgg cgcgcgcagc cggtactgcc agaccaggat gagcagcggt
     4141 tcgccgacga agagcatgca cgcgtaggcc agcggattgc cggactgacg cgcgccgatg
     4201 ccatcgacca cggtgtaggc ggcaatcgcc agcgcgttga gcgcggcggc gcggctggcc
     4261 ggatgggcga gcgcccggcc cggcgtcagc ccgccggcaa aaccgatgc
//
LOCUS       DAOSDX010000125         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7199_length_4308_cov_3.914884, whole genome shotgun sequence.
ACCESSION   DAOSDX010000125 DAOSDX010000000
VERSION     DAOSDX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7199_length_4308_cov_3.914884"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLN02_03835"
     CDS             <1..250
                     /locus_tag="PLN02_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI51482.1"
                     /translation="FDFNTHRDFRAAYEPLVSDDAVRAVTVDFSGVDYLDSSALGMLL
                     MLRDKMGGCGKDVVLSGVQGSVRQVLEIANFSKLFRIV"
     gene            380..1360
                     /locus_tag="PLN02_03840"
     CDS             380..1360
                     /locus_tag="PLN02_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOI51483.1"
                     /translation="MSELLKNIDARTKLAGTNKLEILLFFLGVDQRTGRRETYGINVF
                     KVREVMRTPAITAAPDMPPSVEGMVSLRGALVPVIDLAKYTGISADTPREIMIVTEYN
                     GHTQGFLVEGVDTILRLDWSKMRVPPEMLTAQTGGLVTAVTELEDNRLVMMMDVEKVL
                     AETTSIDNEIMFRSVVPVDRPDATVFYCDDSSVARKQIERTLEAMGVRGLGALNGRQM
                     WDEMEKVAGYAKSVGKPASSLISLVLTDIEMPEMDGYILTKKIKSDPRFANIPVIMHS
                     SLSGMSNQKLGQSVGVDEYVPKFEPQRLSETLARRLKGESEGEAGAIATR"
     gene            1378..2316
                     /locus_tag="PLN02_03845"
     CDS             1378..2316
                     /locus_tag="PLN02_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOI51484.1"
                     /translation="MNLVENKNLLDSVDARTRLAGSNKMEILLFSLGTREIFGINVFK
                     VREVGRTPHITKTPNMPRGVEGLISLRGNVIPVLSLAPFLQLEGVPPGLGKTMMVAEY
                     SKRTLGFLVHEVDRIIRVDWDRVKAPESVLSANHGLITAVIELEGGGLVSILDVEQIL
                     ANAFGEALVVDILPAQVDPDTSVFFVDDSIVARRKISEVLDKLGVRHKHATNGMEAWT
                     RLQAIAAHAQQIGSVLRDEIRLILVDAEMPEMDGYVLTKNIKADPRFEGVPVVMHSSL
                     SSEANRAMGKAVGVDAYVAKFDAEALADTLRPLIER"
     gene            2340..2735
                     /gene="cheY"
                     /locus_tag="PLN02_03850"
     CDS             2340..2735
                     /gene="cheY"
                     /locus_tag="PLN02_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HOI51485.1"
                     /translation="MAADPKTRFLVVDDFSTMRRIVRNLLKELGFTNVDEAEDGAIAL
                     QKLQGGGFEFVVTDWNMPNMDGLTLLQTIRATPHLKHLPVLMITAEAKKENIIAAAQA
                     GASGYIVKPFTAGTLSEKLTKIFEKLGQG"
     gene            2746..3576
                     /gene="cheZ"
                     /locus_tag="PLN02_03855"
     CDS             2746..3576
                     /gene="cheZ"
                     /locus_tag="PLN02_03855"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOI51486.1"
                     /translation="MSASNDSADLEALFDSIAADYAAVPAAPVPPPAAPKVAVDGDND
                     ELQALFDSVAAQSAASQQDKATDDSGGQWPQQEAVFNRIGQMARVLHDTLGQLGYDKL
                     LEKTVAAIPDAKDRLAYVANLTEQAACRVLNATDIAGPLVDDMESRSQSLGQAWDKVF
                     ANQTSPAEFRQLAIETREFLKQQLPEKARATHAQLTEIMMAQDFQDLTGQVIKKIVTL
                     AQELESGLMGLLIEVLPDARKTDEVTSLMNGPVVNAEGRSDVVVNQEQVDDLLDSLGF
                     "
     gene            3589..>4308
                     /locus_tag="PLN02_03860"
     CDS             3589..>4308
                     /locus_tag="PLN02_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOI51487.1"
                     /translation="MSDFSGMEDLLQDFLQEAHDLLSDVDNKLVDLEKTPDDKGLLND
                     IFRGFHTVKGGAGFLNATELVTLCHLTENLFDRLRNGELELTAELMDVIMDATRGVRE
                     MFGELGQGVQPQPADGRVIAALQGALNGEMPAAEAVVQPAPAKPMAAAAPAGAGEPDW
                     AALQAAVTGETAQVAQAAEGELITRAESASVAAKSAKTAAPSPFGRRASDRPSAQGAS
                     SRRAEERGRENTIRVDTSRLDQ"
BASE COUNT          872 a         1214 c         1419 g          803 t
ORIGIN      
        1 ttttgatttc aacacccacc gggatttccg cgccgcgtat gagcccttgg tcagcgacga
       61 tgcggtgcgg gcggtgaccg tggatttttc gggtgtcgat tatctggaca gttccgctct
      121 cggcatgctg ctgatgttgc gcgacaagat gggcggttgc ggcaaggatg tcgtgttgtc
      181 cggggtgcag ggaagcgtca ggcaggttct ggaaattgcc aacttcagca aactgtttcg
      241 cattgtttga ttcatcggtc atgtggtccg tccggggcgg tcttcgccac tgcgggcgtc
      301 gaggttggct gaacggccag ttgcatggta gttttgcccg atacggaacc tgggcaccgt
      361 cccgggcgag gggaaaagaa tgtcggaact actgaaaaat attgatgcgc gcaccaagct
      421 ggcgggaacc aacaagcttg aaatcctgtt gttcttcctc ggggtggacc agcggaccgg
      481 gcgtcgcgaa acatacggca tcaacgtttt caaggtgcgt gaggtgatgc gtacgcccgc
      541 catcacggct gcaccggaca tgccgccttc ggtggagggg atggtgagcc tgcgcggcgc
      601 gctggtgccg gtgatcgatc tggccaagta caccgggatt tccgccgata cgccgcgcga
      661 aatcatgatc gtcaccgagt acaacgggca cacccagggt tttctggttg agggggtcga
      721 taccatcctc cgtctcgact ggagcaagat gcgcgtgccg ccggaaatgc tgacggcgca
      781 gaccggcggc ttggtaaccg cggtgacaga actcgaggac aaccgtctgg tgatgatgat
      841 ggacgtcgaa aaggtgctgg cggaaactac cagcatcgac aacgaaatca tgttccgtag
      901 cgttgttccg gtcgaccgtc cggatgcgac ggttttctac tgcgacgatt ccagcgttgc
      961 ccgcaagcag atcgagcgca ccctggaagc gatgggcgtg cgtggactgg gggcgctcaa
     1021 tggccggcag atgtgggatg aaatggagaa ggtggcaggc tatgccaagt cggtcggcaa
     1081 gccggcgtcg tcgctgatca gcctggtgct gaccgacatc gaaatgccgg aaatggatgg
     1141 ctacatcctg accaagaaga tcaagtccga tccgcgtttc gccaatatcc cggtcatcat
     1201 gcactcctcc ttgtcaggga tgtccaatca gaagctcggc caatcggtcg gggtcgatga
     1261 atatgtgccc aagttcgaac cgcagcgcct gtcggaaacc ctagcccgcc gtctgaaggg
     1321 cgagagcgaa ggcgaagccg gggcgattgc cacgcgttga gccgccggga gacgataatg
     1381 aatctggtcg aaaacaagaa cttgctcgat agcgtcgacg cgcgtacgcg cctggcgggc
     1441 tcgaacaaga tggaaatcct gctgttttcc ctggggaccc gggaaatctt cggcatcaac
     1501 gtgttcaagg tcagggaagt cgggcgcacg ccgcacatca ccaagacccc gaacatgccg
     1561 cgtggcgtcg aagggctgat ttccctgcgc ggcaacgtca ttcccgtgct ctcgctggcg
     1621 cctttcctgc agttggaagg cgtcccgcca gggctgggca agaccatgat ggttgccgaa
     1681 tattccaaac ggaccctcgg tttcctggtc catgaagtcg atcgcatcat ccgcgtcgac
     1741 tgggaccggg tgaaggcgcc ggaaagtgtg ctgagcgcca accacggtct gatcaccgcg
     1801 gtcatcgaac tcgaaggtgg cggtctggtg tcgatcctcg atgtcgagca gatcctggcc
     1861 aatgccttcg gcgaggcctt ggtcgtcgat atcctgccgg cgcaggtcga tccggatacc
     1921 agcgtgtttt tcgtcgacga ttcgatcgtc gcccggcgga aaatctcgga agtgctggac
     1981 aagctcggtg tccggcacaa gcacgcgacc aacgggatgg aagcctggac ccgcctgcag
     2041 gccatcgctg cccacgcgca gcagatcggc agcgtcctgc gcgacgagat tcgcctcatc
     2101 ctggtcgatg ccgaaatgcc ggagatggat ggttatgtgc tgaccaagaa catcaaggcc
     2161 gatccgcgtt tcgaaggggt gccggtggtg atgcactcgt cgctgtcctc ggaggccaat
     2221 cgggcgatgg gcaaggcggt gggggttgat gcctatgtgg cgaagtttga cgcagaggcg
     2281 ctggcggata cgctgcgccc gctgatcgag cgataacgca atgaatactg gagttttgca
     2341 tggctgctga tcccaaaacg cgatttctgg tggtggacga tttttcgacc atgcgccgca
     2401 ttgttcgcaa ccttttgaag gaattgggct ttaccaacgt cgatgaagcg gaggacgggg
     2461 ccatcgccct gcagaagctg caaggggggg gcttcgagtt cgtcgttacc gactggaaca
     2521 tgccgaacat ggatggcctg acgctattgc agacgatccg ggcgacgccg cacctgaaac
     2581 acttgccggt gctgatgatc actgccgagg ccaagaagga aaacatcatc gcggcggcgc
     2641 aggcgggggc cagcggctac atcgtcaagc cgtttacggc gggtaccctg tccgaaaagt
     2701 tgaccaagat tttcgaaaaa ctcggccagg gttaaggggg atagcatgtc agccagtaac
     2761 gattccgccg acctcgaagc gctgttcgat tcgatcgccg cggattatgc cgccgtaccg
     2821 gcggcgccag tgccgccgcc cgccgcgcca aaggtcgccg tggacggcga taacgacgag
     2881 ctgcaggcgc tgttcgacag cgttgcggcg cagagcgcgg ccagtcagca agacaaggcc
     2941 acggacgact cgggtgggca atggccgcag caggaagccg tgttcaaccg gattggccag
     3001 atggcgcgcg tcttgcacga cacgctgggc cagctcggct acgacaagct gctggaaaaa
     3061 accgttgccg caataccgga cgccaaggat cgcttggctt atgtggccaa cctgaccgaa
     3121 caggcggcgt gccgcgttct caacgcgacc gatattgctg gtccgctggt cgatgatatg
     3181 gaaagccgct cgcagagcct tggtcaggcc tgggacaagg tattcgccaa ccagaccagt
     3241 ccggcggagt ttcggcaact ggcgatcgag acccgggagt tcctgaagca gcaattaccg
     3301 gaaaaagcac gggcaacgca tgcccagctg accgaaatca tgatggctca ggacttccag
     3361 gatctgaccg ggcaggtgat caagaagatc gtcactctgg cgcaggaact cgagagcggc
     3421 ctgatgggtt tgcttatcga agtcttgccc gatgcccgca agaccgacga ggtgaccagt
     3481 ctgatgaacg gtcccgtggt caatgccgag ggacgctccg acgtcgtcgt caatcaggag
     3541 caggtcgatg atctgctcga cagtctaggg ttctgaggag gaactgagat gagcgacttt
     3601 tctggcatgg aagatctgct gcaggatttc ctgcaggaag cccacgatct gctctccgat
     3661 gtcgataaca agctggtcga tctggaaaag acgcccgacg acaaaggtct gctgaacgac
     3721 atctttcgcg gttttcacac ggtcaagggc ggcgccggtt ttctcaatgc gaccgaactg
     3781 gtgaccctgt gtcacctcac cgaaaatttg ttcgatcgct tgcgcaatgg cgaactcgaa
     3841 ctgaccgccg aactgatgga cgtgatcatg gacgccacgc gtggcgttcg cgagatgttc
     3901 ggcgaactcg gccaaggggt gcagccgcaa cctgccgatg gtcgggtgat cgccgcgctg
     3961 cagggggcac tcaacggcga gatgcccgca gccgaggcgg ttgtccagcc cgcacctgcc
     4021 aagccgatgg cggcagcggc gccggctggt gccggcgagc cagattgggc cgccttgcag
     4081 gcggcggtga ctggcgaaac cgcccaggtg gcgcaggccg ccgaaggcga gttgatcacg
     4141 cgcgccgagt cggcgtcggt tgccgccaag tcggccaaga cggcggcgcc gtcgccgttc
     4201 gggcggcgag ccagcgatcg tccgtcggcc cagggcgcga gctcgcgacg ggcggaagag
     4261 cgcggccggg agaacacgat ccgggtcgat acctcgcgtc tcgatcag
//
LOCUS       DAOSDX010000126         4303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7208_length_4303_cov_3.337806, whole genome shotgun sequence.
ACCESSION   DAOSDX010000126 DAOSDX010000000
VERSION     DAOSDX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7208_length_4303_cov_3.337806"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLN02_03865"
     CDS             complement(<1..746)
                     /locus_tag="PLN02_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI51488.1"
                     /translation="MSSLNEAALINAGLQAELFDRDLVNRMRAHARANRLSLLDALSR
                     ELGLPHTAFYLALAQVHGLPYARLDQWTLDLGQAARLPATLLKRHPMVPLRNRDGELQ
                     LAIGDPSDQPGIETARRIIGAGTPLAVADPEALAPLLARFAPADARDGTAEEPVALFD
                     RLLRDALLRRASDIHAESVKDGVRIRLRVDGRMIAWGSLLPKALGDGLISRIKVLAGM
                     DIAEARAPQDGSLVYPLGDGEPMEMRVASVP"
     gene            complement(743..1831)
                     /locus_tag="PLN02_03870"
     CDS             complement(743..1831)
                     /locus_tag="PLN02_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOI51489.1"
                     /translation="MAVTIHQLFDTLLASLVEHHASDLHLAEGRVPSWRLHGALAPLG
                     NRQWDAAMMATLLDELLKEQQRQDFRARGTVDLGYSAPGGERFRINVFRSLGRPALVA
                     RHLPSRFASFNELRLPESIRSLAALPDGLVLVSGITGSGKSTTLATLINEINSSAPVH
                     ILTIEDPVEFVHQAKQAIISHRELHTDVADFASAVKASLREDPDVILVGELRDNETMR
                     AALTAAETGHLVFSTLHTADAAGAVERFVGAFSGEEQTVVRHRLSLVLKAVVAQQLLP
                     LHGRPGRVAAVEIMQVTPAVSNLIASGRTAQIYSAIESGRELGMQTFDQSFAGLARDG
                     LIDTDNARRLARDPINFDRLLRAPRSMA"
     gene            complement(1835..2548)
                     /locus_tag="PLN02_03875"
     CDS             complement(1835..2548)
                     /locus_tag="PLN02_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51490.1"
                     /translation="MTPAALKSRLREEMRKWQKLPARDKNLACAVFALILVGVYGGLL
                     WPLTEDRAAKLEYDLQKMAVREKSSNKAAVKEFVPPPSLGGKSPREAEQELEALKRQL
                     ETLRAEVQARQGRFVPMDDSLAMNALKSGLTGLAEAGDMEVTAIEHVYERKEDKDRPP
                     TPELILKAAQANPFQRPLVILRARASFRGLMAFLDGLQALPYIAAPVGCDIVVRVDRD
                     AKTELPIRQWLDVQIKFVV"
     gene            complement(2545..>4303)
                     /locus_tag="PLN02_03880"
     CDS             complement(2545..>4303)
                     /locus_tag="PLN02_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51491.1"
                     /translation="AQADPGQQDRKLPALGGRHGTRKPCLLLAWDVGGLHAAIADGSG
                     ADARQLGQAFSGEGRLRTALAAVLDQFTAQGLARPRQAFLAARHVLPIVADLPVAPDK
                     PRPRAQMRELVQADLEPALAEFGSLWSMGALMQARGYLDGDERQRISQEETLRRQNRQ
                     SQLRFGEIALEIGLIEREGLDECLDLQAELQNLEASVAAGWLGRSEERQALWLVCGVG
                     ERAYREWRETVAEAGLHLAAVLPLAWLASENDAPPADAQRRDASLPRISLELQREEVV
                     AVCRRHERVVAARSEGRVERRLGSDWLTRLIADWASEPRVALEIRAIHGEDDALIAAL
                     AEDLALTTGHPCRALDSTDSRAALWRNLLREGSSRTPGLPRLVDKELRGSPWNNPDLR
                     RLAALGVVLAVIGGVEGYQHLRLHRLEARMAERHQQEQERSRSAQQMAQVSQQLASLG
                     KSLDDTRRQLDPLLADRARLDAVLSMQADLPELLYMLAQSIGDDAVLDEVHNDVTKVS
                     GAAVHVLAWSPSYTGAQAFVSRVAAAVRDRAWGVAQTEINERAGRSGKRGHEISFWLL
                     PEEGELEAVAPSDATEPKR"
BASE COUNT          559 a         1449 c         1595 g          700 t
ORIGIN      
        1 ggtaccgagg cgacgcgcat ttccatcggc tcgccgtcgc ccagcggata gaccaggctg
       61 ccgtcctgcg gtgcccgcgc ttcggcgatg tccatcccgg ccagtacctt gatccggctg
      121 atcaggccgt cgccgagcgc cttcggcagc aggctgcccc aggcgatcat gcggccgtcg
      181 acgcgcagcc ggatgcgcac gccgtccttg acgctttcgg cgtggatgtc ggaggcgcgg
      241 cgcagcagcg cgtcgcgcag caggcggtcg aacagcgcca ccggttcctc ggccgtgccg
      301 tcgcgcgcgt cggccggggc gaagcgggcg agcagcggcg ccagcgcctc ggggtcggcg
      361 acggcgagcg gcgtgccggc gccgatgatg cgccgcgccg tctcgatgcc gggctggtcg
      421 ctggggtcgc cgatcgccag ttgcagttcg ccgtcgcggt tgcgcagcgg caccataggg
      481 tggcgcttga gcagcgtcgc cggcaagcgg gcggcctggc cgaggtcgag cgtccattgg
      541 tcgaggcggg cgtagggcag gccatgcacc tgcgccagcg ccaggtagaa ggcggtgtgc
      601 ggcaggccca gctcgcgcga cagggcatcg agcagggaca gccggttggc gcgggcgtgg
      661 gcgcgcatgc ggttgaccag gtcgcggtcg aacagctcgg cctgcaggcc ggcgttgatc
      721 agcgccgctt cgttgagcga actcatgcca tgctcctggg cgcccgcagc aggcggtcga
      781 agttgatcgg gtcgcgcgcc aagcgacggg cgttgtcggt gtcgatcagg ccgtcgcgcg
      841 ccaggccggc gaaggactgg tcgaaggtct gcatgcccag ctcgcgcccg gattcgatcg
      901 ccgaatagat ctgcgccgtg cgcccggagg cgatcaggtt ggaaacggcc ggtgtcacct
      961 gcatgatttc caccgccgcc acccgccccg gccggccgtg caacggcagc aactgctggg
     1021 cgaccaccgc cttcagcacc agcgacaggc ggtggcggac gacggtctgt tcctcgccgc
     1081 tgaaggcgcc gacgaagcgt tcgacggcgc ccgccgcatc ggcggtgtgc agggtcgaga
     1141 agaccaggtg gccggtttcg gcggcggtca gcgcggcgcg catggtttcg ttgtcgcgca
     1201 attcgccgac caggatgacg tccggatcct cacgcagcga ggctttcacg gcgctggcga
     1261 agtcggcgac atcggtatgc agctcgcggt ggctgatgat cgcctgcttg gcctgatgga
     1321 cgaattcgac cgggtcctcg atggtcagga tatgcaccgg cgcactgctg ttgatctcgt
     1381 tgatcagcgt tgccagcgtc gtgctcttgc cgctgccggt gatgccggag accaggacca
     1441 ggccgtcggg cagtgcggcc aggctgcgga tgctctccgg caggcgcagc tcgttgaagc
     1501 tggcgaagcg cgacggcagg tgacgggcga ccagcgccgg tcggcccagg ctgcggaaga
     1561 cgttgatccg gaagcgctcg ccgccgggcg cgctgtagcc gaggtcgacc gtgcccctgg
     1621 cgcggaaatc ctggcgctgc tgttccttga gcagttcgtc gagcagggtc gccatcatcg
     1681 ccgcgtccca ctgccggttg ccgagcggcg ccagggcgcc gtgcaggcgc cagctcggca
     1741 cccgtccctc ggccagatgg aggtcggagg cgtggtgctc gaccagcgag gcgagcaggg
     1801 tatcgaacag ttggtgaatg gtgacggcca tcttctacac cacgaatttg atctgcacgt
     1861 ccagccactg gcggatcggc agttcggtct tggcgtcgcg gtcgacgcgg acgacgatgt
     1921 cgcagccgac cggcgcggcg atgtagggca gcgcctgcag cccgtcgagg aaagccatca
     1981 ggccgcggaa gctggcgcgg gcgcgcagga tcaccagcgg ccgctggaag gggttggcct
     2041 gcgccgcctt gaggatcagt tccggggtcg gcggccggtc cttgtcctcc ttgcgctcgt
     2101 agacgtgctc gatcgccgtc acctccatgt cgccggcttc ggccaggccg gtcaggccgc
     2161 ttttcagcgc gttcatcgcc aggctgtcgt ccatcggcac gaagcggccc tggcgcgcct
     2221 gcacctcggc gcgcagcgtt tccagttgcc gtttcagggc ttccagctcc tgctcggctt
     2281 cgcgcgggct cttgccgccg aggctgggcg gcggcacgaa ctccttgacc gccgccttgt
     2341 tgctggattt ttcgcgcacc gccatcttct gcaggtcgta ttcgagcttg gccgcgcgat
     2401 cttcggtcag cggccagagc aggccgccgt agacgccgac caggatcagg gcgaacaccg
     2461 cgcaggccag gttcttgtcg cgcgcgggca gcttctgcca tttgcgcatt tcctcgcgca
     2521 ggcgggattt cagggcggct ggcgtcatcg tttcggctcc gtggcatcgc tgggcgctac
     2581 ggcttccagc tcgccttcct cgggcagcag ccagaagctg atttcatggc cgcgcttgcc
     2641 gctgcgcccg gcgcgttcgt tgatctcggt ctgggcgacg ccccaggcgc ggtcgcgcac
     2701 cgccgccgcg acgcggctga cgaaggcctg ggcgccggta tagctgggcg accaggcgag
     2761 gacatggacc gccgcgccgc tgaccttggt gacgtcgttg tgcacttcgt cgagcacggc
     2821 gtcgtcgccg atcgactggg ccagcatgta gagcagttcc ggcaggtcgg cctgcatcga
     2881 cagcacggcg tcgaggcggg cgcggtcggc gagcagcgga tcgagctggc ggcgggtgtc
     2941 gtcgagcgac ttgcccaggc tggccagttg ctggctgacc tgtgccatct gctgcgccga
     3001 gcggctgcgc tcctgttcct gctggtggcg ctcggccatg cgcgcttcca ggcgatgcag
     3061 gcggagatgc tggtagccct cgacgccgcc gatgacggcc aggacgacgc cgagcgcggc
     3121 caggcggcgc aggtccggat tgttccaggg cgagccgcgc aattccttgt ctaccaggcg
     3181 cggcaggccg ggggtccggc tgctgccttc gcgcagcagg ttgcgccaga gcgccgcgcg
     3241 gctgtcggtg ctgtccaggg cgcggcaagg gtggccggtg gtcagcgcca gatcctcggc
     3301 gagggcggcg atgagcgcgt cgtcctcgcc gtggatggcg cggatttcca gcgccacacg
     3361 cggttcgctg gcccagtcgg cgatcagtcg ggtcagccag tcgctgccca gacggcgttc
     3421 gacccgccct tcgctgcgcg cggcgacgac ccgctcgtgg cgacgacaga cggcgacgac
     3481 ttcctcgcgc tgcagttcga ggctgatccg cggcaggctg gcatcgcgcc gctgcgcgtc
     3541 ggctggcggc gcgtcgtttt cgctggccag ccaggcgagc gggagcacgg cggcgaggtg
     3601 gagaccggcc tcggcgaccg tttcgcgcca ttcccggtag gcccgttcgc cgacgccgca
     3661 gaccagccac agcgcctggc gttcctcgct ccgaccgagc cagccggcgg cgacgctggc
     3721 ttcgaggttc tgcagttcgg cctggaggtc caggcattcg tcgagcccct cgcgttcgat
     3781 caggccgatt tccagggcga tttcaccgaa gcgcaactgg ctctggcggt tctgccggcg
     3841 cagcgtttcc tcctgcgaga tgcgttgccg ttcatcgccg tccaggtagc cgcgtgcctg
     3901 catcagcgcg cccatcgacc acaggctgcc gaattcggcc agcgccggtt cgaggtcggc
     3961 ctgcaccaat tcgcgcatct gcgcccgcgg acgcggcttg tccggggcca ccggcaggtc
     4021 ggcgacgatc ggcagtacgt ggcgggcggc caggaaagcc tggcgcggtc gggccaggcc
     4081 ctgtgccgtg aattggtcga ggacggcggc cagcgcggtg cgcaagcggc cttcgccgga
     4141 aaaagcctgg ccgagctggc gggcgtcggc gccgctgccg tcggcgatgg cggcgtgcag
     4201 accgccgacg tcccaggcga gcagcaggca gggtttgcgc gtgccgtgcc ggccgccgag
     4261 ggccggcagc ttccggtcct gctgaccggg atcggcctgt gcg
//
LOCUS       DAOSDX010000127         4292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7237_length_4292_cov_2.936748, whole genome shotgun sequence.
ACCESSION   DAOSDX010000127 DAOSDX010000000
VERSION     DAOSDX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7237_length_4292_cov_2.936748"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLN02_03885"
     CDS             complement(<1..636)
                     /locus_tag="PLN02_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HOI51492.1"
                     /translation="MSLLSLIAVFLIEQFKPLDYRRIVATPLEAWADFIESRFNAGAH
                     HHGIGAWLVAVALPVLLLAGLYYACYAVSPLLAWLLNVGVLYLTMGFRQFSHHYTEIQ
                     LALRLGDLERARQLLGEWQDRPTQGLSAREVARLSIEGALAASHRHVFAVLLWFVLLP
                     GPCGALLYRLALVMRERWTVLGGAAHNEFVTFARQAFALIDWLPLRTTASAF"
     gene            complement(680..1267)
                     /locus_tag="PLN02_03890"
     CDS             complement(680..1267)
                     /locus_tag="PLN02_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOI51493.1"
                     /translation="MSIDLESLRRSLLTNPPAGEVVVEDGATNANLTPAAVLFPIIRR
                     AAGHTVLLTQRTAHLRDHAGQVSFPGGRVEAADDSPLATALRETEEEIGLSRRHVEVL
                     GYLPEYRTGTGFRVVPVVAAVTPPFDLTPDPFEVAEIFEVPLTFLLDQANHQRHSVHL
                     RGALRHFYAMPYAGHFIWGATAGMIRSLSERLVVS"
     gene            complement(1264..1521)
                     /locus_tag="PLN02_03895"
     CDS             complement(1264..1521)
                     /locus_tag="PLN02_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51494.1"
                     /translation="MPQQANEIALLREELEMLMGERQALLRVVGASAALIASLDSKRL
                     PVAAVEAADVVATSINGLSEETLQDALSAVHAEIEEDSAGA"
     gene            complement(1532..2161)
                     /locus_tag="PLN02_03900"
     CDS             complement(1532..2161)
                     /locus_tag="PLN02_03900"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HOI51495.1"
                     /translation="MPHSTLACLACLSSVALAQEVGLAGVMGSKALLMINGAPPRAVP
                     VGSTVDGIKVISVQGDQAVIEIGGRKRPLRVGQHAVGLASDGAGEKIVLTADGQGHFV
                     TIGTINGASVRFLVDTGASMISLGASDARRMGLDFNRGQKGFSQTANGQTMISVVKLD
                     SVRVGGVTLHQVDAAIHQHDLPIALLGMSFLNRMEMQRDGSTMTLKKRY"
     gene            2187..2900
                     /locus_tag="PLN02_03905"
     CDS             2187..2900
                     /locus_tag="PLN02_03905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOI51496.1"
                     /translation="MKPVAIFRHSPTEGPGYFAIFLERQGIPWELIAVDEGAAVPGSA
                     EDFAGLCFMGGPMSVNDPLPWIDSICALIRDAVARDIPVIGHCLGGQLISKALGGQVT
                     KNPVKEIGWGEALAEDNEIARRWLGDFAGRANTVFQWHGETFSIPPGASRLFANAYCA
                     NQMYVLGPHLGMQCHVEMTPEMIATWASQWGDEALAVGDQPSVQTPDEMNGQVGARLP
                     AMRSLSDQLYTAWIANLRR"
     gene            complement(2947..3441)
                     /locus_tag="PLN02_03910"
     CDS             complement(2947..3441)
                     /locus_tag="PLN02_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOI51497.1"
                     /translation="MPSFDFTSEADMVALKNAVDVASRQIENRYDFKGTSAKVELNEK
                     DKVITLWGDSEFQLDQIKDLLFPAMEKKEKDSVKRLDVQKIQSVSGNKSKQEMKIRDG
                     IDSELAKKIVKLIKDAKLKAQASIQGDAVRVSGKKIDELREAIALVRSKITDFPIKDG
                     NYRD"
     gene            complement(3448..3759)
                     /locus_tag="PLN02_03915"
     CDS             complement(3448..3759)
                     /locus_tag="PLN02_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOI51498.1"
                     /translation="MSQYDNVSVVKQANVYFDGKCVSHTVILADGTKKTVGVILPASL
                     TFNTGAPEIMEGVGGACRVKLKGESEWKTYGAGESFNVPGNSSFEIACDEPYHYVCHF
                     G"
     gene            complement(3761..3982)
                     /locus_tag="PLN02_03920"
     CDS             complement(3761..3982)
                     /locus_tag="PLN02_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HOI51499.1"
                     /translation="MEGTLFGFNEEQIADFFSTWGVGAFILYMLFIIGEIAWRSKAGK
                     TGTFVLFFVLSFGMLGFIAKSVIQKIWGI"
     gene            complement(3986..>4292)
                     /locus_tag="PLN02_03925"
     CDS             complement(3986..>4292)
                     /locus_tag="PLN02_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOI51500.1"
                     /translation="LMPRYASLIYNGYWWSPERVALQTLIDHTQQNVNGFVRVKLYKG
                     NVIVVGRDSKTDSLFDSTIATFEDDAGAYDQKDAGGFIKLNALRMRIAANLKARKGG"
BASE COUNT          786 a         1379 c         1365 g          762 t
ORIGIN      
        1 gaaggccgac gccgtcgtcc gcaacggcaa ccagtcgatc aaggcgaaag cctgccgggc
       61 gaatgttacg aactcattat gcgccgcgcc gccgagcact gtccaacgct cgcgcatgac
      121 cagcgccaga cgatagagca gcgcgccgca cggccccggc agcaacacga accagagcag
      181 gacggcaaac acgtggcggt gcgaggcggc caacgcgccc tcgatcgaca gccgcgccac
      241 ttcgcgcgcg ctcagcccct gcgtcggccg atcctgccat tcgcccagca gttggcgggc
      301 gcgctcgaga tcgcccagac gcaaggccag ctggatttcg gtgtaatggt ggctgaactg
      361 gcgaaagccc atggtcaggt agagcacacc gacattgagc aaccaggcga gcagcgggct
      421 caccgcgtaa cacgcgtaat agagtccggc cagcagcagc accggcaggg caaccgcgac
      481 cagccaggcg ccgatcccgt ggtggtgggc gccggcattg aagcgcgatt cgatgaaatc
      541 ggcccaggcc tcgagtggcg tcgcgacgat gcgccggtag tccagcggtt tgaactgctc
      601 gatcaggaaa acggcgatca gcgaaaggag gctcatgggg caaggcggca atcaactgcc
      661 atgtttcttc gtatcggagt caagatacca caagcctttc gctcagcgaa cggatcatgc
      721 cggcggtcgc cccccagatg aaatgtccgg catagggcat ggcataaaaa tggcgcaacg
      781 cgccgcgcag atggaccgaa tgccgctggt ggttggcctg gtcgagcagg aaggtgagcg
      841 gcacctcgaa aatctcggcc acctcgaagg ggtcgggcgt caggtcgaaa ggcggggtca
      901 ccgcggcaac caccggcacg acgcgaaaac cggtgccggt acggtactcc ggcaggtaac
      961 cgaggacttc gacatggcgc cgggaaaggc caatttcttc ctcggtttca cgcaacgcgg
     1021 tagccagcgg cgaatcgtcg gccgcctcga cccgcccgcc gggaaagctg acctggccgg
     1081 cgtggtcgcg cagatgggcg gtacgctgcg tgagcagcac ggtatgaccg gcggcgcgcc
     1141 ggatgatcgg gaacagcacg gcggcaggcg tcaggttggc gttcgtcgca ccgtcctcga
     1201 ccaccacctc gcctgccggc ggattggtca gcaagctgcg gcgcaggctt tccaggtcga
     1261 tgctcatgcg ccggccgagt cctcctcgat ttcggcgtgc accgccgaca gcgcatcctg
     1321 cagggtttcc tcgctcaggc cattgatgct ggtggcgacg acgtcggccg cctcgacggc
     1381 ggccaccggc aagcgcttgc tatcgagcga agcgatcagc gcggcgctgg cgccgaccac
     1441 gcgcaacagc gcctggcgtt cgcccatcag catttccagt tcctcgcgca gcagcgcgat
     1501 ctcgtttgcc tgttgtggca tggtctctct cctaatagcg ctttttcagc gtcatcgtac
     1561 tgccgtcgcg ctgcatttcc atccggttga gaaaactcat cccgagcagg gcgatcggca
     1621 ggtcatgttg gtggatcgcc gcgtcaacct ggtgcagcgt gacgccgccg acgcgcacgc
     1681 tgtccagctt gaccacgctg atcatggtct ggccgttggc ggtttgcgaa aaccccttct
     1741 ggccacggtt gaaatccagc cccatgcgcc gcgcatcgct ggcgccgagg gaaatcatgc
     1801 tcgctccggt gtcgaccagg aaacgcacgg aagcgccgtt gatcgtccca atggtgacga
     1861 aatgcccctg gccgtcggcg gtcagcacga tcttctcgcc ggcaccgtcg gaggccaggc
     1921 caaccgcgtg ctggccgacg cgcaaggggc gcttgcggcc gccgatctcg atcaccgcct
     1981 gatcgccctg caccgaaatc accttgatcc cgtcgaccgt gctgccgacc ggcaccgccc
     2041 gcggcggcgc gccgttgatc atgagcagcg ccttgctacc catgacaccg gccaggccaa
     2101 cctcctgcgc cagagcgacg gagctcagac aggccaggca tgccagcgta gaatgcggca
     2161 tccggaaacg ctttgcgagt acagccatga aacccgttgc catctttcgt cactccccca
     2221 ccgaaggtcc gggctatttt gccatattcc ttgagcgaca agggattccg tgggaattga
     2281 tcgccgtcga cgaaggcgcg gcggtgcccg ggagcgccga ggatttcgct ggcctctgtt
     2341 tcatgggcgg gccgatgagc gtcaatgatc cgctgccgtg gatcgattcg atctgcgccc
     2401 tgatccgcga cgccgtcgcg cgcgacattc cggtaatcgg ccattgcctg ggcggccagt
     2461 tgatcagcaa ggcactcggc ggccaggtga cgaagaaccc ggtcaaggaa atcggctggg
     2521 gcgaggcgct ggccgaggac aatgaaatcg cccgccgctg gctgggcgat ttcgccggcc
     2581 gggcgaacac cgttttccag tggcacggcg agaccttcag cattccgccc ggcgccagcc
     2641 ggctgttcgc caacgcctac tgcgccaacc agatgtacgt gctcggcccg cacctgggca
     2701 tgcagtgcca cgtcgagatg acgccggaga tgatcgccac ctgggccagc cagtggggcg
     2761 acgaggcact ggccgtcggc gatcagccga gcgtgcagac gccggacgag atgaacggcc
     2821 aggtcggcgc gcgcctgccg gcgatgcgct cactctccga ccagctttac accgcctgga
     2881 tcgccaacct gcgccggtaa acgcaagcgg gcggccaagg ccgcccggcg ggtccgctgc
     2941 cggcggtcaa tcgcggtagt tgccgtcctt gatcgggaag tcggtgatct tcgagcggac
     3001 cagcgcgatc gcctcgcgca actcgtcgat cttcttgccg gaaacgcgca ccgcgtcgcc
     3061 ctggatcgag gcctgcgcct tgagcttggc gtccttgatc agcttgacga tcttcttcgc
     3121 cagttcgctg tcgatgccgt cgcggatctt catttcctgc ttcgacttgt tgccggaaac
     3181 gctctggatc ttctgcacgt cgagccgctt gacgctgtcc ttttccttct tttccatggc
     3241 cgggaaaagc aggtccttga tctggtcaag ctggaactcg gaatcgcccc acagcgtgat
     3301 cactttgtcc ttctcgttca actcgacctt ggccgaggtg cccttgaagt cgtagcggtt
     3361 ttcgatctgg cgcgatgcga catcgacggc attcttcaat gccaccatgt cggcttcgga
     3421 ggtgaagtca aatgacggca tgatggctca cccgaagtgg cagacgtagt ggtaaggctc
     3481 gtcgcaggcg atctcgaagc tggaattgcc cggcacgttg aaggattcgc cagcgccgta
     3541 ggtcttccac tcgctctcgc ccttcagctt gacgcggcag gcaccgccga cgccttccat
     3601 gatttccggc gcgccggtgt tgaaggtcag gctggccggc aggatcacgc cgaccgtctt
     3661 cttcgtgcca tcggcgagga tgacggtgtg gctgacgcac ttgccgtcga aataaacgtt
     3721 ggcctgcttg accacggaaa cgttgtcgta ttgcgacatt tcagataccc cagatttttt
     3781 gaatcacgga cttggcgatg aagccgagca tgccgaagga gaggacgaag aagaggacga
     3841 aggtccccgt cttgccggcc ttcgaccgcc aggcgatctc gccgatgatg aacagcatgt
     3901 agaggatgaa ggcaccgact ccccaggtcg agaagaagtc ggcgatctgc tcctcgttga
     3961 agccgaataa ggtgccttcc accgatcaac cgcccttgcg cgccttcagg ttggcggcga
     4021 tgcgcatgcg cagcgcgttc aacttgatga agccgccggc atccttctgg tcgtaggcac
     4081 cagcgtcgtc ctcgaaggtg gcgatggtcg agtcgaacag cgaatcggtc ttcgaatcgc
     4141 ggccgacgac gatcacgttg cccttgtaca gcttgacgcg gacgaagccg ttgacgttct
     4201 gctgggtgtg gtcgatcagg gtctgcagcg cgacgcgctc cgggctccac cagtagccgt
     4261 tgtagatcag gctggcgtag cgcggcatca ga
//
LOCUS       DAOSDX010000128         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7286_length_4272_cov_2.915106, whole genome shotgun sequence.
ACCESSION   DAOSDX010000128 DAOSDX010000000
VERSION     DAOSDX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7286_length_4272_cov_2.915106"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLN02_03930"
     CDS             <1..193
                     /locus_tag="PLN02_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOI51501.1"
                     /translation="TPGDPALGADWPGGFLCPCHGSTFDFAGRVFKNKPAPTNLEIPP
                     HQYLTDSRLIIGSDEFDKT"
     gene            205..333
                     /gene="cydX"
                     /locus_tag="PLN02_03935"
     CDS             205..333
                     /gene="cydX"
                     /locus_tag="PLN02_03935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HOI51502.1"
                     /translation="MWYFAWVLGIGFAVLLAILNTLWGDYEESRAQDEAASRSETA"
     gene            369..761
                     /locus_tag="PLN02_03940"
     CDS             369..761
                     /locus_tag="PLN02_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOI51503.1"
                     /translation="MQIAITSQNRKTITEHAGKCRKFWIYDINQEQIAERRLVELPIE
                     QSFHASHGLPEALTGVEVLISRGMGGNLYQRLISLGIQPLLTDSEDPDQAIADFLSGK
                     LETHSLEDGCHEHEHEHSHHHHQHRHGK"
     gene            871..1263
                     /locus_tag="PLN02_03945"
     CDS             871..1263
                     /locus_tag="PLN02_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51504.1"
                     /translation="MKQTLLNDKYPVFVAEIAKNKTTFQSVPALVEYYKAKIAENPKV
                     QFIGVFDHLAHTTKIGGEIMSGLQAAVDVIFCFGFALPNPQVLAVRPRSIGIADLGDK
                     FVISFMEAPMAPANQAMEAWTLALRNVG"
     gene            1418..1588
                     /locus_tag="PLN02_03950"
     CDS             1418..1588
                     /locus_tag="PLN02_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51505.1"
                     /translation="MKQLQKWFDILAGISEAERHRQALAQAIFNHAGPDLSALATPAC
                     WRRASHVRSGRR"
     gene            1699..2007
                     /locus_tag="PLN02_03955"
     CDS             1699..2007
                     /locus_tag="PLN02_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HOI51506.1"
                     /translation="MKAIDIDETIRLHVKWRRQFLNAFAGGNYVDMPLSDHRACHLLG
                     CKESGLPAEVVAVHQRFHALADEIVELSQNGLGDSADLLLPELNEAAHQFAAALDQLR
                     "
     gene            complement(1999..2271)
                     /locus_tag="PLN02_03960"
     CDS             complement(1999..2271)
                     /locus_tag="PLN02_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51507.1"
                     /translation="MLLFLIGALRAVIEMLGLSLLGLAAMALLAGERRQENMIYRLFE
                     LLTRAPRRLVVSLLPGRERPLLAGVLCFCALFAAWILLAAWRLKLA"
     gene            complement(2275..2523)
                     /locus_tag="PLN02_03965"
     CDS             complement(2275..2523)
                     /locus_tag="PLN02_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HOI51508.1"
                     /translation="MKAVSAYWRKTRRLTAWLLLIWALLTFVVIWFARELNEYQFFGF
                     PFGFYFAAQGILLCYLGIIVYYSQRMSALDAEYEIREE"
     gene            complement(2520..>4272)
                     /locus_tag="PLN02_03970"
     CDS             complement(2520..>4272)
                     /locus_tag="PLN02_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOI51509.1"
                     /translation="LAGTLYLQGFQGLAYVIGWTGGFVLVALLLAPYLRRFGQYTIPD
                     FLCARYGGNAIRLIAVFAAILASFVYVVAQIYGVGVITSRFVSLQFEVGVFVGLAGIL
                     VCSFLGGMRAVTWTQVAQYLILIVAYITPVAILSWNVTGVPVPQAVYGKVLAEVTELE
                     KKLFNSPEEIAVRALYADRADLYAQKIMQLPHSLIQERERLSIEINALKASDAQMRDI
                     VALERQRRELPPDPAQAREQWEKDMYLALDRSKPPTPHAEPFPGETAAERNAQRINFL
                     ALIFCLTVGTAALPHVLMRYYTTPTVAQARQSVFWSLLFITALYVTAPAYAVFAKFEV
                     LSHLAGIPIAKLPGWVAAWSKVGLVSIEDINFDGILQFAELSLNPDVIVLATPEIAGM
                     PYVVSGLVAAGGLAAALSTADGLLLTITSALSHDVYYRVFRPQSSTQFRLVIAKSLLL
                     LVAVLAATVAAQKPGTILSMVAWAFSIAGAALFPALVLGIFWARATRAGAIAGMVVGL
                     LLTLYYILRLEFGGYSWLGFADGTLDPWFHLKSTSAGVFGIVAGFVTNVVVSLLTRQS
                     PQTAEFLACIREPGEGR"
BASE COUNT          933 a         1414 c         1263 g          662 t
ORIGIN      
        1 cacccccggc gatccggcgc tcggcgccga ctggccaggc ggcttcctct gcccctgcca
       61 cggctcgacc ttcgactttg ccggccgcgt tttcaagaac aagccggccc caaccaacct
      121 ggaaattccg ccgcaccagt atctgacgga cagccgtctg atcatcggca gcgacgaatt
      181 cgacaagacc tgaaaggaaa catcatgtgg tatttcgcct gggtactcgg catcggcttc
      241 gccgtgctgc tcgccatcct caacaccctg tggggcgact acgaggaaag ccgcgcgcaa
      301 gacgaagcag cctcgcgctc cgagactgct tgaagcggtc tccccgccgt aatcatcgag
      361 gaaacgacat gcaaatcgcc atcaccagcc agaatcgcaa aacgattacc gaacatgccg
      421 gcaaatgccg caagttctgg atttacgaca tcaaccagga acaaatcgcc gaacggcgac
      481 tggtcgaact accgatcgaa caaagctttc acgccagcca tggtctgccg gaagcactca
      541 caggcgtcga agtcctgatc agccgtggca tgggcggcaa tctgtatcag cgactcatca
      601 gcttgggcat ccagcccctg ctcaccgata gcgaagatcc ggaccaagcc attgccgatt
      661 tcctgagcgg caaactggaa acccactccc tggaggacgg ctgtcacgag cacgagcatg
      721 agcacagcca ccatcatcac cagcatcgcc acggcaaatg acttcagcgt ggactggcgc
      781 tggaagacgc cttcccgctc tgctaacatc cacgatgtca tgccattggc aagccacttc
      841 tttcaacacc tagcggatca tctccctgac atgaaacaaa ccctgctcaa cgacaaatac
      901 cccgttttcg tcgccgaaat cgccaagaac aaaaccacct tccagtcggt tccggcgctg
      961 gtcgaatatt acaaggccaa gatcgccgaa aacccgaaag tccagttcat tggtgtcttc
     1021 gaccacctcg cccacaccac caaaatcggc ggcgagatca tgagcggcct gcaggccgcc
     1081 gtcgacgtca tcttctgctt cggcttcgcc ctgcccaatc cgcaggtcct tgccgtgcgt
     1141 ccccgctcga tcggcatcgc cgacctcggc gacaagttcg tcatcagctt catggaagcc
     1201 cccatggcgc cagccaacca agccatggaa gcctggaccc tggcgctgcg caacgtaggc
     1261 tgactccgag taccacctcc gcaacacgct tcgcccggcc tcgcgccggg ctttttttcg
     1321 cctgcgaaaa aatcgcccga taactgtaca aatcaacagt attgctttac gcttcaacca
     1381 ctgttcgttt atccagttac cgcatcggga gcaattcatg aaacagctgc aaaaatggtt
     1441 cgacatcctc gccggcattt ccgaagccga acgtcaccgg caagcgctgg cccaagccat
     1501 cttcaaccat gccggcccgg acctcagcgc gctggcgaca ccggcatgct ggcgtcgtgc
     1561 cagccacgtg cgctccggcc ggcgctgacg tacttccccc ccacgatggc agccgcgctt
     1621 gcaaagccga cggcaagctg cggcataatg gcgctccgaa acgacccggc agtcgccgtt
     1681 aacaggggga aagcacagat gaaggccatc gatatcgacg aaaccattcg gcttcatgtc
     1741 aaatggcggc ggcaattcct gaatgccttc gccggtggca actacgtcga tatgccgctt
     1801 tccgaccatc gcgcctgcca cctgctgggc tgcaaggaaa gcggcctgcc ggccgaggtc
     1861 gtcgccgtgc accagcgttt ccatgccttg gccgacgaga ttgtcgaact cagccagaac
     1921 ggactcggcg actccgccga cctgctgctg cccgaactca acgaagccgc ccaccagttc
     1981 gcggcggcgc tcgaccagct acgctagctt caggcgccac gcggcaagca ggatccaggc
     2041 cgcgaacagg gcgcagaaac aaagcacgcc tgccagcaac ggacgctcgc gccccggcag
     2101 caggctgacc accagacggc gtggcgcccg ggtgagcagt tcgaacaggc ggtagatcat
     2161 gttctcctgg cgccgctcac cggccagcag ggccattgcc gcaaggccga gcaggctgag
     2221 gcccagcatc tcgatcacgg cgcgcaaggc gccgatcagg aacaacaaca ccgcctattc
     2281 ttccctgatc tcgtattcgg catcgagcgc gctcatgcgc tggctgtaat agacaatgat
     2341 gccgagataa cagagcaata tcccctgcgc cgcgaagtag aagccgaagg gaaagccgaa
     2401 gaactggtat tcgttgagtt cgcgggcgaa ccagatgacg acgaaagtca gtagcgccca
     2461 gatcaacaac agccaggcgg tcagccggcg cgtcttgcgc cagtacgcgg aaaccgcctt
     2521 catcggccct ctcccggttc gcgtatgcag gccaggaact ccgccgtctg cggcgactgc
     2581 cgggtcagca ggctgaccac gacattggtg acgaaaccgg cgacgatgcc gaacacaccg
     2641 gccgaggtcg atttgagatg gaaccagggg tcgagcgtgc cgtcggcgaa accgagccag
     2701 gaataaccgc cgaattcgag acgcaggatg taataaagcg tcagcagcag gccgacgacc
     2761 atgccggcga tggcgccggc gcgcgtcgcc cgcgcccaga agatgccgag caccagcgcc
     2821 gggaaaagtg ccgcaccggc gatcgaaaag gcccaggcga ccatcgacag gatggtcccc
     2881 ggtttctgcg ccgcaaccgt cgccgccaac acggcgacga ggagcaacag cgacttggcg
     2941 atgaccagcc ggaactgggt cgacgactgc ggccggaaga cgcggtaata gacatcgtgc
     3001 gacagcgcgc tggtgatcgt cagcagcagg ccgtcggcgg tggacagcgc cgccgccagg
     3061 ccgccggcgg cgaccaggcc ggacacgaca tagggcatgc cggcgatttc cggcgtcgcc
     3121 aggacgatca cgtccggatt gagcgacagc tcggcgaact gcaggatgcc gtcgaagttg
     3181 atatcctcga tcgacaccag accgaccttg ctccacgccg ccacccagcc cggcagcttg
     3241 gcgatcggga taccggcaag atgagacagc acctcgaact tggcgaacac cgcataagcc
     3301 ggcgccgtca cgtacagcgc ggtgatgaaa agcagcgacc agaacaccga ctgcctggcc
     3361 tgcgccaccg tcggcgtcgt gtaataacgc atcaggacgt gtggcagcgc cgccgtgccg
     3421 acggtcaggc agaagatcag cgccaggaag ttgatgcgct gggcattgcg ttccgccgcc
     3481 gtctccccgg gaaagggttc ggcgtggggg gtcggcggct tgctgcgatc aagcgccagg
     3541 tacatgtcct tttcccactg ctcgcgggcc tgcgccggat cgggcggcaa ctcgcggcgc
     3601 tggcgttcga gagcgacgat atcgcgcatc tgcgcatcgg aagccttcaa ggcgttgatc
     3661 tcgatcgaca gccgttcgcg ttcctggatc agcgaatgcg gcaactgcat gatcttctgc
     3721 gcatagagat cggcccggtc ggcgtagagc gcgcgcaccg caatctcttc cggggaattg
     3781 aagagcttct tctcgagttc ggtgacttcg gccagcacct tgccatacac cgcctgcggc
     3841 accggcacgc cggtgacgtt ccaggacagg atggcaaccg gcgtgatgta ggcgacgatc
     3901 aggatcaggt actgggcgac ctgcgtccag gtcaccgccc gcatgccgcc gaggaaggag
     3961 cagaccagga tgccggcgag gccgacgaag acgccgactt cgaactgcag gctgacgaag
     4021 cggctggtga tgacgccgac accgtagatc tgcgccacga catagacgaa ggaagcgagg
     4081 atggccgcga acacggcgat caggcggatg gcgttgccgc cgtagcgggc gcacaggaaa
     4141 tcgggaatcg tgtattgccc gaagcggcgc aggtaaggcg ccagcagcag ggcgaccagg
     4201 acgaagccgc cggtccagcc gatgacgtag gccagcccct ggaagccctg caggtagagg
     4261 gtgccggcca gc
//
LOCUS       DAOSDX010000129         4257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7324_length_4257_cov_4.117563, whole genome shotgun sequence.
ACCESSION   DAOSDX010000129 DAOSDX010000000
VERSION     DAOSDX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7324_length_4257_cov_4.117563"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLN02_03975"
     CDS             <1..245
                     /locus_tag="PLN02_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOI51510.1"
                     /translation="EFARLFRADFSGADLSAANLEAAKANFAWFVGARLNNANLQESK
                     FFTVNFRHADLSGAFRRFTVFQDADFEACTACPTDW"
     gene            251..712
                     /locus_tag="PLN02_03980"
     CDS             251..712
                     /locus_tag="PLN02_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOI51511.1"
                     /translation="MPLLVSLFLLVVLAAPALAGEVAVAPEVDASALPPLGAQWLTAN
                     PYRGNAVAVDLGRLAYNQACARCHGADAATRAAPAPDLRNLDRYCRRIADAELQAACL
                     RDNDDYFAHSVRHGKVIVGVTHMPPWEGVLKQELAWAIQVFIEAQARGARE"
     gene            complement(787..2568)
                     /locus_tag="PLN02_03985"
     CDS             complement(787..2568)
                     /locus_tag="PLN02_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent methanol/ethanol family
                     dehydrogenase"
                     /protein_id="HOI51512.1"
                     /translation="MHSYKLPRHLLGLALAATLAVAGSPAGATVSDADILNDAKTVGD
                     VVSFGLGTQGQRFSPMTQINTKSVKDLVPAWSMSFGGEKQRGQESQPVIHNGRMFVTA
                     SYSRLFAFDAKTGKKLWKYEHRLPDGIMPCCDVINRGAALYGDLVIFATLDAQLVAMN
                     QETGKVAWKQKVGDYAAGYSATAAPIIAKGKLITGVSGGEFGAVGRIDARDPLTGKLL
                     WTRPTVEGHMGYRYDAEGKEIENGISGTLNATWTGDLWKTGGAATWNGATYDPETNLI
                     FAGTGNPAPWNSHLRPGDNLFSSSTVAIDVDTGKIVWHYQNTPHDGWDFDGVNEFVSF
                     DYKDPKTGKLIKAGGKADRNGFFFVNDRTNGKLLNAFPFVRKITWASSINLETGRPNY
                     IEEGRPGDPAAGVDGKKGKVVFSAPSFLGGKNQQQMAYSPQTGLFYVPANEWSMDIWN
                     EPVSYKKGAAYLGAGFTIKAIHDDYIGVMRAVDPATGKIVWENKNYAPLWGGALATAG
                     GLVFYGTPEGYLKGVDAKTGKEIWSFQVGTGIVAPPVTWEQDGEQMLAVTTGWGGAVP
                     LWGGDVAKRVNFLEQGGSVWVFKLHKR"
     gene            complement(2666..2845)
                     /locus_tag="PLN02_03990"
     CDS             complement(2666..2845)
                     /locus_tag="PLN02_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51513.1"
                     /translation="MATALDEQQAANTLPAGAANPLRKFGTGVINFSTRQRTGHERLS
                     PLLALSMGACGDPAR"
     gene            2945..3922
                     /locus_tag="PLN02_03995"
     CDS             2945..3922
                     /locus_tag="PLN02_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOI51514.1"
                     /translation="MMLKKTLYAVATALALSAGLAQAEPAKPECIAPAKPGGGFDLTC
                     KLAQSALQNSKLLEAPMRVTYMPGGIGAVAYNTVIANRPAEGGTIVAYSGGSFLNLAL
                     GKFGKYSEDDVRWLAAVGADYGAVIVKADSPYRNLKELIEATRKEPGKVVFGAGGSVG
                     SQDWMKSALIAREAGVDPKSMRYVAFEGGGEAITALMGGHVHAYSGDASEVLGHLKGG
                     RIKVLAILAPERLKGELANIPTAIEQGYNVEWTITRGFYMGPKVSDADYKWWLAAFEK
                     LQGSPEFDKLRAERGLFPFNLTGEKLTASVKREVARYKLLAKELGLAAQ"
     gene            3970..>4257
                     /locus_tag="PLN02_04000"
     CDS             3970..>4257
                     /locus_tag="PLN02_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOI51515.1"
                     /translation="MSERILGGILLFVSVIGIWLARSFEVTISYEPVGPRAFPITVLI
                     LLGISAIALMFEKKSDTVWAPPAVLLRIGALFLVVLAYAWLFDKLGFILATS"
BASE COUNT          670 a         1411 c         1410 g          766 t
ORIGIN      
        1 tcgaattcgc ccgcctgttc cgtgccgact tttccggtgc cgacctgagc gccgccaatc
       61 tcgaagcggc caaggcgaat ttcgcctggt tcgtcggcgc ccgcctgaac aacgccaacc
      121 tgcaggaaag caagttcttc acggtgaatt tccgccacgc cgatctgagc ggcgccttcc
      181 gccgcttcac ggtcttccag gatgccgact tcgaggcctg cacggcctgc ccgaccgatt
      241 ggtgatgccc atgccgctgc tcgtttccct cttcctcctc gtagtcctgg ccgcgccggc
      301 gctggctggc gaggtcgccg tcgcgcccga agtcgacgcc tcggccttgc cgccgctcgg
      361 cgcgcagtgg ctgacggcca atccctatcg tggcaacgcg gttgccgtcg atctcggccg
      421 tctcgcctac aaccaggcct gcgcccgctg ccacggcgcc gacgcggcga cccgggcggc
      481 gcccgccccc gacctgcgca acctggaccg ctactgccgg cgcatcgccg acgccgagct
      541 ccaggccgcc tgcctgcgcg acaacgacga ctatttcgcg cacagcgtgc gccacggcaa
      601 ggtcatcgtc ggcgtcaccc acatgccgcc ctgggaaggc gtgctcaagc aggagctggc
      661 ctgggcgatc caggtcttca tcgaagccca ggcgcgcgga gcgcgcgaat gaggcgctgg
      721 tcgtcggcgc aagaaaaacg gcgcccgggc cgtagccggg gcgccgctgt tgccgggcgt
      781 cgttgttcag cgcttgtgca gcttgaacac ccacaccgaa ccgccctgtt cgaggaagtt
      841 cacgcgcttg gcgacatcgc cgccccacag cggcacggcg ccgccccagc cggtggtgac
      901 ggcgagcatc tgttcgccgt cctgttccca ggtgaccggc ggtgcgacga tgccggtgcc
      961 gacctggaag gaccagattt ccttgcccgt ctttgcatcg acgcccttca ggtagccctc
     1021 cggcgtgcca tagaagacca gaccgccggc ggtggccagc gcaccacccc agagcggggc
     1081 gtagttcttg ttctcccaga cgatcttgcc ggtggccggg tcgaccgcgc gcatgacgcc
     1141 gatgtagtcg tcgtggatgg ccttgatggt gaagccggca ccgagatagg cagcgccctt
     1201 cttgtaggag accggttcgt tccagatgtc catcgaccac tcgttggccg gcacgtagaa
     1261 gaggccggtc tgcgggctgt aggccatctg ctgctggttc ttgccgccga ggaaggacgg
     1321 cgccgagaag acgaccttgc ccttcttgcc atcgacgccg gctgccggat cgcccggacg
     1381 cccttcctcg atgtagttgg gacggccggt ttccaggttg atgctgctcg cccaggtgat
     1441 cttcctgacg aaggggaagg cgttcagcag cttgccgttg gtccggtcat tgacgaagaa
     1501 gaagccgttg cggtcggcct tgccgccggc cttgatcagc ttgccggtct tcgggtcctt
     1561 gtagtcgaag gaaacgaact cgttgacacc gtcgaagtcc cagccgtcgt gcggggtgtt
     1621 ctggtagtgc cagacgatct tgccggtgtc gacgtcgatg gcgacggtgg acgaggagaa
     1681 gaggttgtcg cccgggcgca gatggctgtt ccacggggcc gggttgccgg tgccggcgaa
     1741 gatcaggttg gtttccggat cgtaggtggc gccattccag gtggcggcgc cgccggtctt
     1801 ccacaggtcg ccggtccagg tggcgttgag ggtgccggag atgccgttct cgatttcctt
     1861 gccttcggcg tcgtagcggt agcccatgtg gccttcgacg gtcgggcgcg tccacagcag
     1921 cttgccggtc agcgggtcgc gggcgtcgat gcggccgacg gcgccgaatt cgccgccgga
     1981 aacgccggtg atcagcttgc ccttggcgat gatcggcgct gcggtggccg agtagccggc
     2041 ggcgtagtcg ccgaccttct gcttccaggc gaccttgccg gtctcctggt tcatggcgac
     2101 cagttgcgcg tcgagcgtgg cgaagatgac caggtcgccg tacagcgcag caccgcggtt
     2161 gatcacgtcg cagcagggca tgatgccgtc gggcaggcgg tgctcgtact tccacagctt
     2221 cttgccggtc ttcgcgtcga aggcgaacag gcggctgtaa gaggcggtga cgaacatccg
     2281 gccgttgtgg atcaccggct gcgattcctg gccgcgctgc ttctcgccgc cgaaggacat
     2341 cgaccaggcg ggaaccaggt ccttgaccga cttggtgttg atctgcgtca tcgggctgaa
     2401 gcgctgaccc tgggtgccga ggccgaagct gaccacgtcg ccgacggttt tcgcgtcgtt
     2461 gaggatgtcg gcatcgctga ccgtggcgcc ggccgggctg ccggcgacgg cgagcgtcgc
     2521 cgcgagggcg agcccgagca ggtgccgggg aagtttgtaa ctgtgcatgg aacgtctcct
     2581 tggttgtgcg ttgattccgt cgccgcagtg accccttgcc ggggtcggtc ggcgacgaag
     2641 ctagccgact gcaagccctg tgccatcacc gcgcagggtc gccgcaagcg cccatcgaca
     2701 aggcaagcag ggggctgagc cgttcgtgcc cggtgcgctg ccgggtgctg aaattgatca
     2761 cacctgttcc aaacttgcgc agcgggtttg ctgcgccagc aggcagggtg ttcgcggcct
     2821 gctgttcgtc gagcgcagtc gccatccctg aaagccaatc gaaagccagt ctccttatct
     2881 tgcgttgcca tgcagggcct gcgcatcagc gccctgcacc gatcaacaat caaaggagac
     2941 gatcatgatg ctcaagaaaa ccctgtacgc cgttgccacc gccctggcat tgagcgccgg
     3001 cctggcccag gccgaaccgg ccaaacccga gtgcatcgcc ccggccaagc cgggcggcgg
     3061 tttcgacctg acctgcaagc tcgcccagag cgccctgcag aacagcaagc tgctcgaagc
     3121 gccgatgcgc gtgacctaca tgcccggcgg catcggcgcg gtggcctaca acaccgtgat
     3181 cgccaaccgg ccggccgaag gcggcacgat cgtcgcctat tcgggcggtt ccttcctcaa
     3241 cctggcgctc ggcaagttcg gcaaatactc cgaggacgac gtcaggtggc tggccgccgt
     3301 cggcgccgat tacggtgcgg tgatcgtcaa ggccgattcg ccgtacagga acctcaagga
     3361 actgatcgag gcgacgcgca aggaaccggg caaggtggtg ttcggggccg gcggttcggt
     3421 gggtagccag gactggatga agtcggcact gatcgcccgc gaggccggcg tcgatccgaa
     3481 gtcgatgcgc tacgtcgcct tcgaaggcgg tggcgaagcc atcaccgcgc tgatgggcgg
     3541 ccacgtgcac gcctactccg gcgatgcctc ggaagtgctc ggtcacctca agggcggccg
     3601 catcaaggtt ctcgccatcc ttgccccgga acgcctgaag ggcgaactgg ccaacatccc
     3661 gacggcgatc gagcagggct acaacgtcga gtggacgatc acccgcggct tctacatggg
     3721 gcccaaggtg tcggacgccg actacaaatg gtggctcgcc gccttcgaga agctgcaggg
     3781 cagcccggag ttcgacaagc tgcgcgccga gcgcgggctg ttcccgttca acctgaccgg
     3841 ggaaaaactg accgccagcg tcaagcgtga ggtcgcccgc tacaagctgc tggccaagga
     3901 actcggtctc gccgcccagt aatttcgctc aacggccccg ctgcggcggg gcctttcccg
     3961 gagattgcca tgagtgaaag aatcctcggc ggcatcctgc tgttcgtcag cgtcatcggc
     4021 atctggctgg cgcgcagctt tgaagtgacc atttcctacg aaccggtcgg cccgcgcgcc
     4081 tttccgatca ccgtcctgat cctgctcggc atcagcgcca tcgccctgat gttcgaaaag
     4141 aagagcgaca cggtctgggc gccgcccgcc gtgctgctgc gcatcggcgc gctgttcctc
     4201 gtcgtgctgg cctatgcctg gctgttcgac aagctcggct tcatccttgc caccagc
//
LOCUS       DAOSDX010000130         4257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7326_length_4257_cov_3.074488, whole genome shotgun sequence.
ACCESSION   DAOSDX010000130 DAOSDX010000000
VERSION     DAOSDX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7326_length_4257_cov_3.074488"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PLN02_04005"
     CDS             complement(<1..1248)
                     /locus_tag="PLN02_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOI51516.1"
                     /translation="MNVLFEEDGGFKAGSILADNDSSLQVEMPSGKRSKIKAASVLLR
                     FAQPAAGALLEQATPLAAEIEADFLWECVSDGEFSFLDFARDYYGHDPAPVEATAVLL
                     ALHAAPVYFHRKGKGRFRKAPADILAAALANLEKKRQQALAMEGWLAELKEFRLPPEI
                     AVLTDALLYAPDRNKPETKAFETACAETGLSAAQLLFRCGAIKSAYHLHYKRFLHEQF
                     PRGTAFLALDAPPLPIDLARAEVRAFSIDDANTTEIDDALSVTWLPGIGSRIGIHIAA
                     PGLAIEHGSPLDGVARARLSTVYMPGNKITMLPDAVVQNYTLAGGTDCPAVSLYLTVN
                     EQYEVLSHESCIERVHIAENLRHHDVEPWFNSETVGAALPDKPFARELLHLWHFANAC
                     EGRRGKPSAMQGVNDYNFAIAGDL"
     gene            complement(1256..1822)
                     /locus_tag="PLN02_04010"
     CDS             complement(1256..1822)
                     /locus_tag="PLN02_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOI51517.1"
                     /translation="MDGLILYLHGFCSSPASWKARLLGEEMTRRGLAARFVCPALSPV
                     PAQAIADLSRFLDSVGRPVTLIGSSLGGHYASYLAAQFGLPAALINPAVVSALSAEQF
                     IGEHANFHSGERFVFTAEHAAQLAAQVARPDPARLWLLLETGDEVLDYRAAQAHWFGS
                     RQSVFEGGDHSFTRFPELMPQLIEFAGL"
     gene            complement(1810..2631)
                     /locus_tag="PLN02_04015"
     CDS             complement(1810..2631)
                     /locus_tag="PLN02_04015"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOI51518.1"
                     /translation="MDPILLFKALILGIVEGLTEFLPISSTGHLIILGSLLDYTDEQS
                     KVFKIVIQLGAILAVCWDFRERIGKALGGLGSDPVQQRFAGLLLVGFLPAAVLGLLFH
                     STIKAYLFNPLSVAGALIVGGFLILYIERKAYHPRISSVDDMRWPDALKVGFAQAMAM
                     FPGTSRSGATIMGGLIFGLSRKTATEFSFFLAIPTMFAATVYDVYKNWSLLHMADLPV
                     FAVGFLAAFVAAMLTVKALLRYVSNHDFTVFAWYRIVFGVIVLLTAEMGWVEWTA"
     gene            complement(2663..2911)
                     /locus_tag="PLN02_04020"
     CDS             complement(2663..2911)
                     /locus_tag="PLN02_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOI51519.1"
                     /translation="MTAICRPGCGACCIAPSISSLAKPAGVPCRHLDAERRCRIFGRP
                     ERPACCSGLQASAEMCGETPLQALQWLADLEAATRPQG"
     gene            complement(2908..>4257)
                     /locus_tag="PLN02_04025"
     CDS             complement(2908..>4257)
                     /locus_tag="PLN02_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOI51520.1"
                     /translation="YLENRFARAQTYVRANQNYLLAQLRIWLGRLVFWGCGIVVGLAA
                     VLFALLSDDAAKGFANLAALYPWLPFVVTPLAGAALLWVTRNVFPGAQGSGIPQVIAE
                     MKRPAVAEWRPLVSLRIAVGKILVGVAAVGSGFSFGREGPTVQIGASLMAAAGRFMPA
                     TLRIHRQHLLVAGGAAGIAAAFNAPLAGIVFAIEELSRSVESRLSGVVIAAIVLAGVI
                     AQHFLGSDNYFGRVVVFGESGELLTTILTCAVVTGIAGGLFARMLIVSATAWKGSLAN
                     LRNAHPYVFVACCGVLLASLGWVTGGLTWGSGFAETKALLIGQSDLPWYFGPAKFVAT
                     VVSYLSGLPGGIFAPSLAIGAGLGQDLVPVLGGEAFPTTIIILCMVGFLAAATQAPIT
                     AFIIVMEMVDGYSLVIGLMATALIASAVSRMFSKSLYTTLAEHMLRQQVPVDSPPPK"
BASE COUNT          874 a         1316 c         1404 g          663 t
ORIGIN      
        1 gagatcgccg gcgatcgcga agttgtagtc gttcacgccc tgcatcgccg acggtttgcc
       61 gcgccggcct tcgcaggcgt tggcgaaatg ccagaggtga agcagttcac gagcgaaggg
      121 tttgtccggt agcgcggcgc ctacggtctc gctgttgaac cagggctcga catcgtggtg
      181 acgcaggttt tcggcgatgt gcacgcgttc gatgcaggac tcgtgcgaca gcacttcgta
      241 ctgctcgttg accgtcaggt agagcgatac cgccgggcaa tcggtaccgc cggccagcgt
      301 gtagttctgg accacggcat cgggcagcat ggtgatcttg ttgcccggca tatacacggt
      361 cgacaggcgg gcgcgggcga cgccgtcgag cggcgaaccg tgttcgatgg cgaggccggg
      421 cgccgcgata tggatgccga tgcggctgcc gataccgggc agccaggtga ccgacagtgc
      481 gtcgtcgatc tcggtggtgt tggcgtcgtc gatcgaaaag gcgcggacct cggcacgcgc
      541 caggtcgatc ggcagcggcg gcgcatcgag cgccaggaag gcggtgccgc gcgggaactg
      601 ctcgtgcagg aaacgcttgt agtgcaggtg gtaggccgac ttgatggcgc cgcacctgaa
      661 cagcaactgg gcggcggaga ggccggtttc ggcgcaggcc gtttcgaagg ccttggtttc
      721 cggcttgttg cggtccggcg cgtagagcag cgcgtcggtc agcacggcga tctccggcgg
      781 caggcggaat tccttcagtt cggcgagcca gccttccatc gccagcgctt gctgacgctt
      841 tttttcaaga ttggccagag cagctgcgag aatgtcggcg ggcgccttgc ggaagcggcc
      901 cttgcccttg cggtgaaaat agacgggcgc ggcgtgcagg gcaagcagca ccgccgtcgc
      961 ttcgaccggt gcggggtcgt gtccgtagta atcacgggca aaatccagaa acgaaaattc
     1021 gccatcgctg acacactccc agaggaaatc cgcctcgatc tctgcggcaa gtggcgtcgc
     1081 ctgctcgagc agggcaccgg ccgccggctg ggcaaagcgc agcaggacgc tggccgcctt
     1141 gatcttgctg cgtttgccgg agggcatttc gacctgcaac gaactatcgt tgtcggccag
     1201 gatgctgccc gccttgaaac cgccatcttc ttcaaacaat acattcatcg gcggattata
     1261 acccggcaaa ttcaatgagt tgcggcatca attcgggaaa gcgcgtgaag ctgtgatcgc
     1321 cgccctcgaa gacgctttgc cggctaccga accagtgcgc ctgggcggcg cgatagtcga
     1381 gcacctcgtc accggtttcc agcagcagcc agagccgtgc cggatcgggc cgggctacct
     1441 gggcggcgag ctgcgccgcg tgctcggcgg tgaacacaaa acgctcgccg ctgtgaaaat
     1501 tggcgtgctc gccgatgaac tgctcggctg acagcgcgct gactaccgcc ggattgatca
     1561 gtgccgccgg caggccgaac tgcgccgcca gatagctggc gtaatgaccg cccagcgagg
     1621 aaccgatcag cgtcaccggg cgcccgacgc tatcgagaaa gcgcgacagg tcggcaatcg
     1681 cctgcgcagg aaccggcgac aaggctggac agacgaagcg cgcagccagc ccgcggcggg
     1741 tcatttcttc accgagcagg cgggccttcc acgacgccgg cgacgaacag aagccgtgca
     1801 gataaaggat caggccgtcc attcgaccca acccatctcc gcggtgagca gcacgatcac
     1861 gccgaagacg atgcggtacc aggcgaatac cgtgaagtcg tggttcgaca cataacgcaa
     1921 cagggccttg acggtcagca tcgcggcgac gaaggcggcc aggaaaccaa ccgcgaagac
     1981 cggcaggtcc gccatgtgca gcaaggacca gttcttgtag acgtcataga cggttgccgc
     2041 gaacatcgtc ggaatggcga ggaagaagga aaactcggtc gccgtcttgc gcgacagacc
     2101 gaagatcagg ccgcccatga tcgtcgcgcc ggaacgcgag gtcccgggaa acatcgccat
     2161 cgcctgcgcg aaaccgacct tcagggcgtc cggccaacgc atgtcatcga cgctgctgat
     2221 gcggggatgg taggccttgc gttcgatgta gagaatcagg aaaccgccaa cgatcagcgc
     2281 gccagcgacg ctcaacgggt tgaacagata ggccttgatc gtgctgtgga aaagcaggcc
     2341 gaggacggcg gcagggagga aaccgaccaa caacagaccg gcgaagcgct gttgcaccgg
     2401 atcgctgccg agaccgccca acgccttgcc gatgcgttcg cgaaaatccc agcagacggc
     2461 gagaatcgcc cctagctgga tgacgatctt gaataccttg ctttgctcgt cggtgtagtc
     2521 gagcaagctg ccgaggatga tcaggtggcc ggtcgaggaa atcggcagga actcggtcaa
     2581 accctcgacg atgccgagga tcaatgcttt gaagaggaga atcgggtcca tggagggatg
     2641 cgctatgaca agagcgcgca ttttacccct gcggacgcgt cgccgcctcc aaatcggcca
     2701 gccattgcag cgcctgcagc ggcgtttcgc cgcacatttc cgccgaagct tgcaggccgc
     2761 tgcagcaggc gggccgctcc ggtcgcccga aaatgcggca acgccgctcg gcgtcgagat
     2821 ggcgacaggg tacgcctgcc ggtttggcga gcgaggagat cgacggggcg atgcagcaag
     2881 caccgcagcc ggggcggcag atggccgtca tttcggcggc gggctgtcga ccggcacttg
     2941 ctggcgcagc atgtgctcgg ccagtgtcgt gtacagcgac ttcgaaaaca tccgggacac
     3001 cgccgaggca atcagggcgg ttgccatcag gccgatcacc agcgagtaac catcaaccat
     3061 ctccatgacg atgatgaagg cggtgatcgg cgcctgggtc gcggcggcca gaaagccgac
     3121 catgcagaga atgatgatgg tggtcggaaa tgcctcgcca ccgaggacgg gaaccagatc
     3181 ctggccgagg ccggcgccaa tcgccagcga aggcgcaaag atcccgcccg gcagaccact
     3241 cagatacgag acgacggtgg caacgaattt cgcgggcccg aagtaccaag gcaggtcgct
     3301 ctgtccgatc agcagcgcct tggtctcggc aaagccgcta ccccaggtta gcccgcccgt
     3361 cacccagccc agggaggcca gcaggactcc gcaacaggcc acgaacacat agggatgagc
     3421 attgcgcagg ttggccagtg aacctttcca ggcggtggcc gaaacgatca gcatgcgtgc
     3481 gaagaggccg cccgcaatgc cggtgacgac cgcgcaggtc aggatcgtcg tcagcagttc
     3541 gccgctttcg ccgaacacga cgacgcgacc gaaatagttg tcactgccga gaaaatgttg
     3601 agcgatcacc ccggccagga cgatcgccgc gatgacgacg ccgctcagac gggattccac
     3661 ggagcgcgac aactcctcga tggcaaatac gatccccgcc agcggggcgt tgaaggctgc
     3721 ggcaatgccg gcggcgccgc cggcaaccag caagtgctgg cggtggatgc gcaaggtggc
     3781 tggcatgaaa cgtccagcgg cggccatcag gctggcgcca atctgtaccg tcggcccttc
     3841 ccgaccgaag gagaagccgc tccccaccgc ggcaacgcca accaggattt tgccgacggc
     3901 aatccgtaac gataccagcg gcctccactc cgccacggcg ggccgcttca tttcggcgat
     3961 tacctgcgga ataccgctcc cctgcgcgcc gggaaagaca ttgcgggtca cccacaacag
     4021 cgcggctcca gccagcggcg tgacgacgaa gggtaaccac gggtaaagtg ccgccaagtt
     4081 cgcgaagccc ttcgctgcgt cgtcactgag cagggcgaag agtactgcgg ccaacccgac
     4141 gacgatgccg catccccaga aaaccagtcg tcccagccag atcctgagct gcgcgagcag
     4201 gtagttctga ttggcgcgaa cgtaggtttg ggcacgtgcg aaccggtttt ccaggta
//
LOCUS       DAOSDX010000131         4251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7336_length_4251_cov_3.869638, whole genome shotgun sequence.
ACCESSION   DAOSDX010000131 DAOSDX010000000
VERSION     DAOSDX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7336_length_4251_cov_3.869638"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLN02_04030"
     CDS             <1..221
                     /locus_tag="PLN02_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOI51521.1"
                     /translation="RRDLLFTNRAAWLAVGVLLLLQLAFVYLPFMNAAFATTPLALRH
                     WFAPLAIGLFIFLGMELEKRWQSSRQRT"
     gene            307..3744
                     /locus_tag="PLN02_04035"
     CDS             307..3744
                     /locus_tag="PLN02_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51522.1"
                     /translation="MPSLRSLNSATWLRVLPILLGGWLLAGAIEHLDQRQGERTAMRV
                     TELRLQGAASRLEKTLKQMLQVNYDFAAALPADYSIDEARLRPLAERLVASHPRLINI
                     TLSRQMEVVFVHPFAGNEAVFGMNYANRPYVMTGVERAMATRDTVLTGPVKLVQNQRL
                     GLVGRTPVYFAAGDGKAQVFQGIVSCAIDFEGVLADAGLLGATPSFSLAIRGRDGSGA
                     QGAPFFGDPALFKQPHVAIDLDLPGGQWRLAAVPTNEASPLRNWTIRGIIALLTLLLA
                     VRAGAGAGNLTGIAIRLPGKRQIGLRGFLLGALFLVLLPIIGISGWIAYRNAGDAADQ
                     FTRTAAGSLSQRAHNRVSNFFDVPRRVVAFNVERARAGLLDTNDRPRMMQDFLLQIRQ
                     QPLLTFISAGMPDGEYYAGSRPPLGNDKGLRLLYARLAEQRVMQIYRVDDAARRTSLV
                     GYGNDEFDARTRPWYIAARDSGRMAWYPPYRYTINDQQGAYDTMGIGMSAPLYDPAGR
                     FIGVTSADVALSQLSNLLRELTDESGGIAFLADSENRLLASSTPAAILDSGQRFRLAE
                     SGDPLLQAAGALMQPDGGAEGSRFFADEGARHLLEWHTIALENGPALSIGVILPQSRF
                     DVLTAGMLRNLAYLALVIGVFGIVVGLLATDWVSRPLARLARKASRMAAGDWQTVIAP
                     ETPIREVRTLSSALADMARQLQRHTEHLERQAAELRSGNERLQAEVAERMKSEARIQA
                     LNVDLEIANQTLLLAKEAADSANKAKSAFLANMSHELRTPMHGIMGMVSLVRARVSEP
                     KLQHQLDRAMEAASRLLLILNEILDLSKIEADRLTLDRVEFKLAPVLDNVAALLANKA
                     ADKGLRLEIRPLPANGGQTFVGDALRLGQVLINLVGNAVKFTERGSIDLAVDIVAESA
                     DDALLRFTIRDSGIGIAPEDQRRLFNAFEQADSSTTRKYGGTGLGLAICKRLVAMMGG
                     DIGVDSQPGVGSTFWFTLRLGRTAHEPPALPATADDHEIRLRQQHAGARILLAEDEPI
                     NQEVTRELLEQVALVVDLAENGEMAVSMAGEQQYDLILMDMQMPLLNGLDATRAIRAG
                     GPNRNTPILAMTANAFSEDRQRCLDAGMNEHLGKPVDPEQLYLLLLRCLEAGKQAAN"
     gene            3910..>4251
                     /locus_tag="PLN02_04040"
     CDS             3910..>4251
                     /locus_tag="PLN02_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51523.1"
                     /translation="MTGKEQSAAWRRWLKACLLQFALLAGLVAAQESPRVLVLLSYHH
                     GYTWEDRILDGFEEWGGVVAGRPALHVEWMDTKRHPDPAARQRFAAYLKEKHAGRRFD
                     LLVAVDDNALEF"
BASE COUNT          757 a         1582 c         1240 g          672 t
ORIGIN      
        1 tgcgccgcga cctgctgttc accaatcgcg ctgcctggct ggcggttggc gtgctgttgc
       61 tgctgcaact ggctttcgtc tacctgccgt tcatgaacgc cgccttcgcc accacgccgc
      121 tcgccttgcg ccactggttc gccccgctgg cgatcggcct cttcatcttc ctcggcatgg
      181 aactggaaaa gcgctggcag tcatcacgac agcgcacctg aacattctgc cgccgcttcc
      241 agttgcttgc cggggacgaa tcccggaaca tggccgcatg aaatcatccc tgtgcggcaa
      301 gcaagcatgc cctccctgcg ttccctgaac agcgccacct ggctgcgggt actgcccatc
      361 ctcctcggcg gctggctgct ggctggcgcc atcgagcacc tcgaccagcg ccagggcgaa
      421 cggacggcca tgcgcgtcac cgaactgcgc ctgcagggcg ccgcgtcgcg cctggaaaaa
      481 acgctcaagc agatgctgca ggtcaattac gatttcgccg ccgcgctgcc cgccgactac
      541 agcatcgacg aagcccgcct gcggccgctc gccgaaaggc tggttgccag ccacccacgc
      601 ttgatcaaca tcacgctgtc gcgccagatg gaagtcgtct tcgtccatcc ctttgccggc
      661 aacgaagccg ttttcggcat gaactacgcc aaccggccgt acgtcatgac cggcgtcgag
      721 cgggcgatgg cgacccgcga tacggttctc accgggccgg tcaaactggt ccagaaccag
      781 cgcctcgggc tggtcggccg cacccccgtc tatttcgccg cgggcgacgg caaggcgcag
      841 gtattccagg gcatcgtctc ctgcgccatc gatttcgagg gcgtccttgc cgatgccggc
      901 ctgctcggcg ccacgccgag cttctccctc gccatccgtg gccgcgacgg cagcggcgcc
      961 caaggcgccc ccttcttcgg cgatccggca ctgttcaagc agccacatgt ggcgatcgac
     1021 ctcgacctgc caggcggcca gtggcgactc gccgcggtgc cgacaaacga ggccagcccg
     1081 ctgcgcaact ggacgatacg cggcatcatt gccctgctca cgctgctcct tgccgtccgc
     1141 gccggtgccg gcgccggcaa tctgaccgga atcgccatcc ggctgccggg aaagcgccag
     1201 atcggcctgc gcggcttcct gctcggcgcc ctcttcctgg tcctcctgcc gatcatcggc
     1261 atcagcggct ggatcgccta ccgcaatgcc ggcgacgccg ccgaccagtt cacccggacc
     1321 gctgcgggaa gtctgagtca gcgcgcccac aaccgcgtca gcaatttctt cgatgtcccc
     1381 cgacgggtcg tcgccttcaa cgtcgagcga gcccgcgccg ggctcctcga caccaacgac
     1441 aggccacgga tgatgcagga cttcctcctg cagatccggc aacaacccct gctcaccttc
     1501 atctccgccg gcatgccgga tggcgaatac tacgccggca gccggccacc gctcggcaac
     1561 gacaagggcc tgcgcctgct ttatgcgcgt cttgccgaac aacgcgtcat gcagatttat
     1621 cgcgtagacg acgccgcccg ccgcacctcg ctggtcggct acggcaacga cgaattcgac
     1681 gcccgcaccc gtccctggta catcgccgcc cgcgattcgg ggcgcatggc ctggtaccca
     1741 ccctaccgct acaccatcaa cgaccagcag ggtgcctacg acaccatggg catcggcatg
     1801 tcggcaccgc tctacgatcc agccggccgc ttcatcggcg tcaccagcgc cgatgtcgca
     1861 ctctcccagc tctccaatct gcttcgtgaa ttgaccgacg aaagcggtgg cattgccttc
     1921 ctcgccgata gcgaaaaccg cttgctcgcc agctcaacgc cggctgccat cctcgactcg
     1981 ggccagcgct tccgcctcgc cgaaagtggc gaccccctgt tgcaggcagc cggtgcgctg
     2041 atgcagcccg acggtggcgc cgagggctcg cgcttcttcg ccgacgaagg cgcccgccat
     2101 ctgctcgaat ggcacaccat cgccctggaa aacggccccg ccctgagcat cggcgtgatc
     2161 ctgccgcaat cgcgtttcga cgtgctgacc gccggcatgc tgcgcaacct cgcctacctc
     2221 gccctggtca tcggcgtctt cggcatcgtc gtcgggctgc tcgccaccga ctgggtttcc
     2281 cgcccgctcg cccgcctcgc ccgcaaggcc tcgcgcatgg ccgccggcga ctggcagacg
     2341 gtaatagcgc cggaaacgcc gatccgcgaa gtccgcacgc tgtcctcggc gctcgccgac
     2401 atggcccgtc agttgcagcg ccacaccgaa cacctcgaac gccaggccgc cgaactgcgc
     2461 agcggcaacg aacgcctgca ggccgaggtt gccgagcgga tgaaatcgga agcgcgcatc
     2521 caggcgctca acgtcgacct cgagatcgcc aaccagaccc tgctcctcgc caaggaagcc
     2581 gccgactcgg caaacaaggc caaatcggcc ttcctcgcca acatgagcca cgaactgcgc
     2641 accccgatgc acggcatcat gggcatggtt tcgttggtcc gcgcccgggt cagcgagccc
     2701 aagctgcaac accagctcga ccgcgccatg gaagccgcca gccggctgct gctgatcctc
     2761 aacgaaatcc tcgacctgtc caagatcgaa gccgaccggc tgaccctcga ccgggtcgaa
     2821 ttcaagctgg cgccggtact cgacaacgtc gccgcactgc tcgccaacaa ggccgcggat
     2881 aagggcctcc gtctcgaaat ccggccgctg cccgccaacg gcgggcagac cttcgtcggc
     2941 gacgcgctgc gcctcggcca ggtcctcatc aacctggtcg gcaacgcggt caaattcact
     3001 gaacgcggct ccattgacct tgccgtcgac atcgtcgccg aaagcgccga cgacgccctg
     3061 ctccgcttta ccatccgcga ctccggcatc ggcatcgccc ccgaagacca gcggcgcctg
     3121 ttcaacgcct tcgaacaggc cgacagttcg actacccgca aatacggcgg caccggtctc
     3181 ggcctcgcca tctgcaagcg gctggtcgcc atgatgggcg gcgacatcgg cgtcgacagc
     3241 cagcccggcg tcggcagcac cttctggttc accctccgcc tcgggcggac ggcgcatgaa
     3301 ccgccagcac tgccagccac ggccgacgac cacgaaatcc gcctgcgcca gcagcatgcc
     3361 ggtgcccgca tcctgctcgc cgaggacgaa ccgatcaatc aggaagtcac ccgggaactg
     3421 ctcgaacagg tcgccctcgt cgtcgacctc gcggaaaacg gcgaaatggc ggtcagcatg
     3481 gccggcgaac aacaatacga cctgatcctg atggacatgc agatgccgct actgaacggc
     3541 ctcgacgcca cccgggcgat ccgcgccggc ggccccaacc ggaacacacc gatcctcgcg
     3601 atgaccgcca acgccttcag cgaagaccgc cagcgctgcc tggacgccgg catgaacgag
     3661 cacctcggca aaccagtcga ccccgagcag ctctacctgc tcctgctgcg ctgcctggag
     3721 gccggcaagc aggctgcgaa ctgaatcatc aaggcatccc caacaacgtc ccgatggcga
     3781 aacacggggc gagcgggtat cgttgatgaa aagtctgcag ccgccatatc gcgctagccg
     3841 gagatgcgat cacaagcagt ggcctggccc acctggggcg tcagacacaa aggagactgg
     3901 gagcgagaga tgacgggaaa agaacaatcc gctgcctggc ggcgctggct gaaggcctgc
     3961 ctgttgcagt tcgcgctgct tgccgggctg gttgcggccc aggaatcgcc ccgagttctc
     4021 gtcctcctct cctaccacca tggctacacc tgggaagacc gcatcctcga cggtttcgag
     4081 gaatggggcg gcgtcgtggc cggtcgaccg gcgctgcacg tcgagtggat ggataccaag
     4141 cgccatccgg acccggcagc gcgccaacga tttgccgctt acctgaagga aaaacatgcc
     4201 gggcggcgct tcgatctgct ggtcgccgtc gatgacaacg cgctggagtt c
//
LOCUS       DAOSDX010000132         4247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7344_length_4247_cov_3.082538, whole genome shotgun sequence.
ACCESSION   DAOSDX010000132 DAOSDX010000000
VERSION     DAOSDX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7344_length_4247_cov_3.082538"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLN02_04045"
     CDS             <1..405
                     /locus_tag="PLN02_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOI51524.1"
                     /translation="LACAVTEIAGSSAWFERGFVTYSNAAKSELLGVPEITIERHGAV
                     SEATARAMAEGALAHSPAHWAVAITGIAGPAGGSADKPVGTVCFAWAGREGGCLAQTR
                     HFAGDRAAVREQSVRHALSGLLNLLAGVDCQA"
     gene            complement(407..850)
                     /locus_tag="PLN02_04050"
     CDS             complement(407..850)
                     /locus_tag="PLN02_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOI51525.1"
                     /translation="MPRITLSALLLLCTPFLAVAGDWRVNEATRGNFTDTRDAIVTAI
                     ENRGLVINATSHIGDMLERTGLDLGKSRRIYEQAEILEFCSASLSRQMMESDPHDIVN
                     CPFTIAIYTLAGNPQTTWVGYRKQSGKSAAALERMLSEIVAEALH"
     gene            complement(898..4179)
                     /locus_tag="PLN02_04055"
     CDS             complement(898..4179)
                     /locus_tag="PLN02_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOI51526.1"
                     /translation="MQNAADLADSFTEDDIVDWLGEAELAKGRPYVDLVRDLNIDDGE
                     MHAVVPGSARQPYRVEALIAPDSGSGRLIARCTCPVGRNCKHVAAVLLKAIEQRNPKE
                     RVSGSVLSWVEDLRRASIAVAKKKARPASARQQLYYILKWSPDRRRFGIELRKGKYPE
                     NAEEWWKVDRALVTPPQFVTEEDLGILRLIWAERGHDSGLRAYGLGPKHGAEILERMA
                     ATNRLYPAVDLGLPLHGGAARPASVGWQIDGQGLQRPYFKSEPPTELVIAVDPPWYVD
                     LTEGEAGPLTVTGNPAVIGRLFSLPPLSAKEAALVADALSELAPELPLPAEDASARLR
                     QIDSPPQPVLQLETLRTHGHRPWRKYAYSFGGGLFDVAHVLFRYGDADLKPQHENEFV
                     TLPEGETVRVKRRPDEEKKALACLVESGLQKIPANTLNTFGSPPEGIYGLAEEAAWHD
                     FMQRCVPQLRECGWQVEFPEDFRHHVVEVDAWDAELIETDDGWFDLDMGILVEGERLP
                     LAPLLATLFRRDPRWLEGIELARIPDEEGIELLTPAGKRLRVPAGRIKPLAMTLIDLF
                     DGFGGGPTLRLSRFDAPRLAELTDTSRWEFRGQNDVLALAERLQAAQGIVDIAPPQGL
                     RLDLRPYQKEGLAWLQFLREQKLCGILADDMGLGKTAQTLAHLLLEKEGGRLDRPALV
                     ILPTSLIFNWKSEAARFAPDLRVLSLHGPERKQRFAEIAEHDVVLTTYPLLWRDADEL
                     MRHSYHLLILDEAQTVKNAQSRSAEAVRKIDARHRLCLTGTPLENHLGELWSQFDFLL
                     PGFLGSNKQFTRHWRTPIEKQGDIDRRDLLARRIRPFILRRKKEDVAQELPPKTIIVR
                     SVELEGGQRDLYETVRAAMDAKVRDEIAQRGFARSQIVILDALLKLRQVCCDPRLVAA
                     SSARKVSERAKLDLLMAMLPELVDENRHILVFSQFTSMLALIEQELQAAGLAYVKLTG
                     ETVDRETPIRRFQEGGAPIFLISLKAGGVGLNLTAADTVIHYDPWWNPAVENQATDRA
                     HRLGQDKPVFVYKLIVSGSIEEKILALQERKAELAAGILSEDRQVDIKFGDDDLAALF
                     EPLPG"
BASE COUNT          637 a         1310 c         1529 g          771 t
ORIGIN      
        1 cttgcctgtg cggtcacgga aatcgccggc agctcggcct ggttcgagcg cggcttcgtc
       61 acctattcca acgccgccaa aagcgagctg ctgggcgtac cggaaatcac catcgaacgc
      121 catggcgcag tttcggaagc cacggcccgt gccatggctg agggagcact cgcccacagc
      181 ccggcacact gggcggtcgc aattaccggt atcgccggtc cggccggcgg ttcggccgac
      241 aaaccagtgg gcacggtctg ctttgcctgg gccgggcgag agggcggttg cctggcgcag
      301 acgcggcact ttgcagggga tcgggccgcg gtgcgtgagc agtcggtcag gcacgccctg
      361 agcggtctgt tgaatctgct tgcgggcgtc gactgccagg cctgagtcaa tgcagggctt
      421 cggctacgat ctcggagagc atccgttcga gggcggcggc cgatttgccg gactgctttc
      481 ggtaaccgac ccaggtggtc tgcggatttc cggcaagggt atagatggcg atcgtaaacg
      541 gacagttcac gatgtcgtgc gggtcgcttt ccatcatctg ccgcgacagc gaagccgagc
      601 agaactccag gatttccgcc tgctcgtaga tgcgccgtga tttgccgaga tcgaggccgg
      661 tgcgctcgag catatcgccg atatggctgg tggcgttgat caccaggccg cggttttcga
      721 tggcggtgac gatggcgtcg cgggtgtcag tgaaattgcc gcgcgtcgcc tcgttgaccc
      781 gccagtcgcc agcaacggcg aggaaggggg tgcaaagcag caggagcgca ctcagggtga
      841 tgcggggcat gggcttctcc ggtgtcatgg tttcggccaa ttgtaagggc ggtcgggtca
      901 gccggggagt ggttcgaaca gggcggcgag gtcgtcgtcg ccgaatttga tgtcgacctg
      961 gcggtcctcg gagaggatgc cggcggcgag ttcggccttg cgttcctgca gggcgaggat
     1021 cttttcctca atgctgccgc tgacgatcag cttgtagacg aagaccggct tgtcctggcc
     1081 gaggcggtgc gcgcggtcgg tggcctggtt ctcgaccgcc gggttccacc acgggtcgta
     1141 gtggatcacg gtgtcggcgg cggtcaggtt gaggccgacg ccgccggcct tcaggctgat
     1201 caggaagatc ggcgcgccac cttcctggaa gcggcggatc ggcgtctcgc ggtcgaccgt
     1261 ctcgccggtc agcttgacgt aggcgaggcc ggcggcctgc agctcctgct cgatcagcgc
     1321 cagcatgctg gtgaattgcg agaagaccag gatgtgccgg ttttcgtcga ccagctcggg
     1381 gagcattgcc atcagcaggt cgagcttggc ccgttcgctg accttgcgcg ccgaactggc
     1441 ggcgaccagg cgcgggtcgc agcagacctg gcgcagcttg agcagggcgt cgaggatgac
     1501 gatctggctg cgcgcgaagc cgcgctgggc gatctcgtcg cgcaccttgg catccatcgc
     1561 cgcgcgcaca gtctcgtaaa ggtcgcgctg gccgccttcg agttcgacgc tgcggacgat
     1621 gatcgtcttc ggcggcaatt cctgggcgac atcctccttc ttgcggcgca ggatgaaggg
     1681 gcggatgcgg cgggccagca ggtcgcggcg gtcgatgtcg ccctgttttt cgatcggcgt
     1741 ccgccagtga cgggtgaatt gcttgttgct gccgaggaag ccggggagca ggaagtcgaa
     1801 ctggctccac aattcgccga gatggttctc caacggcgtg ccggtcaggc acaggcgatg
     1861 ccgcgcgtcg atcttgcgca ccgcttcggc gctgcggctc tgcgcgttct tcaccgtctg
     1921 cgcctcgtcg aggatgagca ggtggtagct gtggcgcatc aactcgtcgg cgtcgcgcca
     1981 gagtagcgga taggtggtga ggacgacgtc gtgctcggca atttcggcga agcgctgctt
     2041 gcgctccggg ccgtgcagag acaggacgcg caggtcggga gcgaagcgcg ctgcttcact
     2101 cttccagttg aagatcagcg aagtcggcag gattaccagc gccggccggt cgaggcggcc
     2161 gccttctttt tccagcagca ggtgggccag cgtctgcgcc gtcttgccga ggcccatgtc
     2221 gtcggcgagg atgccgcaca gtttctgttc gcgcaggaac tgcagccagg ccaggccttc
     2281 cttctggtat ggtcgcaaat cgaggcgcag gccctgcggc ggcgcgatgt cgacgatgcc
     2341 ctgcgccgcc tgcaggcgtt cggccagcgc cagcacgtcg ttctggccgc ggaactccca
     2401 gcggctggtg tcggtcagtt cggccaggcg cggcgcgtcg aagcgcgaca ggcgcaaggt
     2461 cgggccaccg ccgaagccgt cgaagagatc gatcagcgtc atcgccagcg gcttgatgcg
     2521 gccggccggg acgcgcaggc gcttgccggc cggggtcagc agttcgatgc cttcctcgtc
     2581 ggggatgcgc gccagttcga taccctccag ccagcgcggg tcgcggcgga agagcgtggc
     2641 cagtagcggg gccagcggca ggcgctcgcc ttcgaccagg atgcccatgt cgaggtcgaa
     2701 ccagccatcg tcggtttcga tcagttcggc gtcccaggca tcgacttcga cgacatggtg
     2761 gcggaagtct tcagggaact cgacctgcca gccgcattcc cgcaactgcg gcacgcagcg
     2821 ctgcatgaag tcgtgccagg cggcttcctc ggccaggccg tagatgccct ccggcgggct
     2881 gccgaaggtg ttgagcgtgt tggctggaat cttctgcaag ccgctctcga cgaggcaggc
     2941 cagggctttc ttttcctcgt cggggcggcg tttgacgcgt accgtctcgc cttccggcaa
     3001 ggtgacgaat tcgttttcgt gctgcggttt gaggtcggcg tcgccgtagc ggaacagcac
     3061 gtgggcgaca tcgaacaggc cgccgccgaa gctgtaggca tacttgcgcc agggccggtg
     3121 gccgtgcgtg cgcagggttt ccagttgcag caccggttgc ggcgggctgt cgatctggcg
     3181 caggcgggcg ctcgcgtcct cggccggcag cggcaactcg ggcgccagtt cggagagggc
     3241 gtcggcgacc agcgccgctt ccttggccga cagcgggggc aacgagaaca gtcggccgat
     3301 caccgccggg ttgccggtga cggtcagcgg tccggcctcg ccctcggtga ggtctacgta
     3361 ccagggcgga tcgacggcga tcaccagttc ggttggtggc tccgacttga aataggggcg
     3421 ctgcagaccc tggccgtcga tctgccagcc gacgctggcc ggccgcgcgg cgccgccgtg
     3481 cagcggcagg ccgaggtcga cagccggata caggcggttc gtcgccgcca ttcgttcgag
     3541 gatttcggcg ccgtgtttcg gtcccaggcc gtaggcgcgc aggccgctgt cgtggccgcg
     3601 ttcggcccag atcaggcgca ggatgccgag atcctcttcg gtaacgaatt gcggcggggt
     3661 gaccagggcg cggtcgacct tccaccattc ctcggcgttt tccgggtact tgcccttgcg
     3721 cagttcgatg ccgaagcggc gccggtcagg cgaccatttc aggatgtagt agagctgctg
     3781 acgggcgctc gccgggcgtg ccttcttctt ggcgacggcg atcgaagcgc ggcgcagatc
     3841 ctcgacccag gacaggacgc tgccgctgac ccgttccttc gggttgcgct gttcgatggc
     3901 cttgagcagg acggcggcaa cgtgcttgca gttacggccg accgggcagg tgcagcgagc
     3961 aatcagccgg ccgctgccgc tatccggggc gatcagtgcc tcgacgcggt agggttggcg
     4021 cgccgaaccc ggaaccacag cgtgcatctc gccgtcgtcg atattcaggt cgcgcaccag
     4081 atccacgtag ggacggccct tggcaagctc ggcctcgccc agccagtcga cgatgtcgtc
     4141 ctcggtgaag ctgtcggcga ggtcggcggc gttctgcatc acaggctcag gtagtggctg
     4201 ccgagcagcg cggcgagcag cagggcgatg atcgccagca acaggtt
//
LOCUS       DAOSDX010000133         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7368_length_4238_cov_3.063830, whole genome shotgun sequence.
ACCESSION   DAOSDX010000133 DAOSDX010000000
VERSION     DAOSDX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7368_length_4238_cov_3.063830"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /gene="gyrA"
                     /locus_tag="PLN02_04060"
     CDS             complement(<1..1938)
                     /gene="gyrA"
                     /locus_tag="PLN02_04060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOI51527.1"
                     /translation="MDQFAKETLPISLEDEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLYAMHELNNDWNKAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQDFSLRYML
                     VDGQGNFGSIDGDNAAAMRYTEVRMAKIGHQLLEDLDKETVDFGPNYDGSEQEPLVLP
                     TRIPNLLINGSSGIAVGMATNIPPHNLKEVVAGCLALLENPAISIDELIEHIPAPDFP
                     TAALIYGVMGVREGYKTGRGRVVMRARTHFEDIGKGDRQALIVDEIPYQVNKKSLIEK
                     IAELVNEKKIEGISDIRDESDKSGMRIVIELKRGEVGEVILNNLYKQTQLQDTFGMNM
                     VALLDGQPRLLNLKQMLEAFLRHRREVVTRRTVFELRKARERGHVLEGLAVALANVDE
                     MIAIIKAAPTPPDAKRELMARSWTSPVVEEMLKRAASDASRPEGLSVDFGLTPNGYRL
                     SDVQAQEILQMRLQRLTGLEQDKIVNEYKEVMDVITDLLDILARAERVTEIIVQELSA
                     IRQQFGDKRRSEIVENTQDLSLEDLITPQDVVVTLSHTGYVKSQPLDEYRAQRRGGRG
                     KQAAGTKEDDFIDKMFVANTHDYILCFSSRGRVYWLKVYEVPQGGRASRGKPIVNLFP
                     LEEGEKINAILSVKEFDENRYV"
     gene            2041..3387
                     /locus_tag="PLN02_04065"
     CDS             2041..3387
                     /locus_tag="PLN02_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HOI51528.1"
                     /translation="MTPTPEKIDTLIEARWIATVETDAILHNHAIAVRGGHILDILPA
                     AEARQRYQAAEQHRLEQHILIPGLINLHTHAAMTLLRGIADDKPLMEWLQHHIWPAEG
                     ALVSAPFVLDGTRLACAEMLRGGITCFNDMYFFPEAAAQAATEFGMRAALGITTLEFP
                     GNYASDADDYIAKGLAAREACRDNPLLSFCLAPHAPYTVSDRTFQRIATLAEQLDLPI
                     HCHIHETRQEVADGLQQHGQRPLARLQALAQLGPGFIGVHAVHLDDKDIRLLADAGCS
                     IAHCPTSNLKLASGIARIAEMDAAGINIGLGTDGAASNNRLDMFGEMRLAALLAKGSS
                     GDAAALPAARALRMATLNGAKALGLDSITGSIACGKQADLCAVSLADLECQPCYDPLS
                     HLVNVAGREQVSHVWVAGNCLLEHKTLRLGKEFRLDASAALWQNKAEVCTGKAHAP"
     gene            3431..4099
                     /locus_tag="PLN02_04070"
     CDS             3431..4099
                     /locus_tag="PLN02_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI51529.1"
                     /translation="MIKNIAKKSLAVALLAGIGFAAVAQERVYVIDQRDVVAKSGFGL
                     CWRTGYWTPAAAAADKAGCECDRDLLPKAACEGKPVAPAAAAAAAVAGVKPTGEKVTV
                     AADALFDFNKAVLRPEGKAKLDELVAKAGQIKLEVILAVGHTDRIGGAAYNQKLSEKR
                     AAAVKEYLVAKGIEANRVYTEGKGETQPVTGDKCGKSEKKSKSLIECLQADRRVDIEV
                     IGTK"
BASE COUNT          771 a         1414 c         1272 g          781 t
ORIGIN      
        1 gacatagcgg ttttcgtcga actccttgac cgagaggatg gcgttgatct tctcaccctc
       61 ttccagcggg aacaggttga cgatgggctt gccacggctg gcgcggccgc cctgcggcac
      121 ttcgtagacc ttgagccagt agacgcggcc gcggctggag aaacagagga tgtaatcgtg
      181 cgtgttggcg acgaacatct tgtcgatgaa atcgtcttcc ttggtgccgg ccgcctgttt
      241 gccgcgcccg cctcgacgtt gcgcgcggta ctcgtccagc ggctgcgact tgacgtaacc
      301 ggtatgcgac agcgtgacga cgacgtcctg cggcgtgatc aggtcttcca gcgacagatc
      361 ctgcgtgttc tcgacgatct cgctacgccg cttgtcgccg aactgctgac ggatggcgga
      421 aagctcttgc acgatgattt ccgtcacgcg ctcggcccgg gccagaatat cgagcaggtc
      481 ggtgatgacg tccatcactt ccttgtattc attgacgatc ttgtcctgtt cgaggccggt
      541 cagacgttgc aggcgcatct gcaggatttc ctgcgcctgc acgtcggaca gtcgatagcc
      601 attgggggtc aggccgaagt cgaccgacag accttccgga cgcgaggcat cggaagcggc
      661 acgcttcagc atctcctcga ctaccggcga ggtccaactg cgcgccatca actcacgctt
      721 ggcgtcaggt ggtgtcggcg ctgccttgat gatggcgatc atctcatcga cattagccag
      781 cgccacggcc aggccttcca acacgtggcc gcgctcgcgc gccttgcgca actcgaacac
      841 ggtgcgacgc gtgacgacct cgcgccggtg gcgcaagaag gcttccagca tctgtttcag
      901 gttgaggaga cgcggctggc cgtcgagcaa agccaccatg ttcatgccga aggtgtcctg
      961 cagttgcgtc tgcttgtaca ggttgttgag gatgacctcg ccgacctcgc cgcgcttcag
     1021 ctcgatgacg atgcgcatgc ccgacttgtc ggactcgtcg cggatgtcgg agatgccttc
     1081 gatcttcttc tcgttgacca gttcggcgat cttctcgatc agcgacttct tgttcacctg
     1141 atacggaatc tcgtcgacga tcagcgcctg gcggtcgccc ttgccgatat cctcgaagtg
     1201 ggtgcgggca cgcatgacga cgcggccgcg gccggtcttg tagccttcgc gcacacccat
     1261 cacgccgtag atcagcgcgg cggtcgggaa atccggcgcc gggatgtgct cgatcagttc
     1321 gtcgatcgag atcgccgggt tctccaggag cgccaggcag ccggcgacga cttccttgag
     1381 gttgtgcggc gggatgttgg tcgccatacc gacggcgata ccggacgaac cgttgatcag
     1441 caggttcggg atacgggtcg gcaggaccag cggttcctgc tcggagccgt cgtagttcgg
     1501 cccgaaatcg acggtttcct tgtccagatc ttcgagcagt tgatgcccga tcttggccat
     1561 gcgcacttcg gtgtaacgca tcgccgccgc gttgtcgccg tcgatcgaac cgaagttgcc
     1621 ctggccgtcg accagcatgt agcgcagcga aaagtcctgg gccatgcgga cgatggtgtc
     1681 gtacaccgcc gaatcgccgt gcgggtggta cttaccgatg acgtcgccga cgatacgcgc
     1741 cgacttcttg tacgccttgt tccagtcgtt gttcagctcg tgcatcgcgt agagcacgcg
     1801 gcgatgcacc ggcttcaggc cgtcgcgcgc atccggcagc gcccggccga cgatcacgct
     1861 catcgcgtaa tcgaggtagg agcgccgcat ctcgtcttcg aggctgatcg gcagggtttc
     1921 tttggcgaat tggtccatgt ctctgctatc cgcgccgggc tggccggcac tatgtttggt
     1981 tgaagcacag gtaatttgct attttaccac gccgccaccg gcctaccgca gcccatttcc
     2041 atgacaccga cacccgaaaa aatcgacacc ttgatcgaag cccgctggat tgccaccgtg
     2101 gaaaccgacg caatcctgca caaccacgcg atcgcggtac gcggcgggca catcctggac
     2161 atcctgccag ccgccgaagc gcgtcagcgt taccaggctg ccgaacagca ccgcctcgaa
     2221 caacacatcc tgatccccgg cctgatcaac ctccacacgc acgccgcgat gacgctgctg
     2281 cgcggcatcg ccgacgacaa accgctgatg gaatggctgc agcaccacat ctggccggca
     2341 gagggagcgc tggtttcggc gcccttcgta ctcgacggca cgcggctggc ctgcgccgaa
     2401 atgctgcgcg gcggcatcac ctgtttcaac gacatgtatt tcttccccga agccgccgcc
     2461 caggccgcga cggagttcgg catgcgcgcc gcgctcggca tcaccacgct ggaattcccc
     2521 ggcaactacg ccagcgacgc cgacgactac atcgccaagg gcttggccgc ccgcgaagcc
     2581 tgccgcgata acccgctgct cagcttctgc ctggcgccgc acgcgcccta caccgtgtcc
     2641 gaccgcacct ttcagcgcat cgccacgctg gccgagcagc tcgacctgcc aatccactgt
     2701 cacatccacg aaacccggca ggaagtcgcc gatggcctgc agcagcacgg ccagcgcccg
     2761 ctcgcccgac tgcaggcact ggcccagcta ggccccggct tcatcggtgt acacgccgtc
     2821 cacctcgacg acaaggatat ccgactgctc gccgacgccg gctgcagcat cgcccactgc
     2881 ccgacctcca acctgaagct ggccagcggc atcgcccgta tcgccgagat ggacgctgcc
     2941 ggcatcaata ttggcctcgg caccgatggc gccgccagca acaaccgcct cgacatgttc
     3001 ggcgaaatgc gcctggccgc cctgctggcc aagggcagca gcggcgacgc cgccgccctg
     3061 ccggcagcga gagcactgcg catggccacc ctgaatggcg cgaaggcgct gggtctggac
     3121 agcatcaccg gttccatcgc ctgcggcaaa caagccgacc tctgcgcagt ctcgctggct
     3181 gatcttgaat gccagccctg ctacgatccg ttgtcgcacc tggtgaatgt cgccggccgc
     3241 gaacaggtca gccatgtctg ggtggccgga aactgtctgc tagagcacaa aactttacgg
     3301 cttggcaagg aatttcgctt ggatgcatcc gccgcgctat ggcagaataa agcggaagtc
     3361 tgcactggga aggcgcatgc gccctgatgc aaaccgctga aatttccaat ttcttcaacg
     3421 agggaagacc atgatcaaga acatcgccaa gaaatccctg gccgttgccc tgctggcggg
     3481 tatcggcttt gccgccgtcg ctcaggaacg tgtctatgtc atcgaccagc gcgacgtcgt
     3541 cgccaagagc ggtttcggcc tctgctggcg caccggctac tggaccccgg ctgccgccgc
     3601 cgccgacaag gccggttgcg aatgcgaccg cgacctgctg ccgaaggccg cctgtgaagg
     3661 caagccggtc gcgccggccg ctgctgccgc agccgccgtc gccggcgtca agccgactgg
     3721 cgaaaaggtc accgtcgccg ctgacgcgct gttcgacttc aacaaggctg tgctgcgtcc
     3781 ggaaggcaag gccaagctcg acgaactggt tgccaaggcc ggtcagatca agctcgaagt
     3841 gatcctcgcc gtcggccaca ccgaccgcat cggcggtgcc gcctacaacc agaagctgtc
     3901 cgagaagcgc gctgccgcgg tcaaggaata cctggttgcc aagggtatcg aagccaaccg
     3961 cgtctacacc gaaggcaagg gcgaaaccca gccggtcacc ggcgacaagt gcggcaagag
     4021 cgagaagaag tcgaagtcgc tgatcgagtg cctgcaggcc gatcgccgcg tcgacatcga
     4081 agtcatcggc accaagtaat cctgccgctc ttgcggtaaa agaaaggccc tgccccggca
     4141 gggctttttc acatctggac aacatgaacc gtcgcctgct ttcctgctgc ctcctcgtcc
     4201 ttgccttgtc cgccgctctt gcccaggcgg cggaacgg
//
LOCUS       DAOSDX010000134         4208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7427_length_4208_cov_2.969420, whole genome shotgun sequence.
ACCESSION   DAOSDX010000134 DAOSDX010000000
VERSION     DAOSDX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7427_length_4208_cov_2.969420"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PLN02_04075"
     CDS             <1..1375
                     /locus_tag="PLN02_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-hexose 2,3-dehydratase family protein"
                     /protein_id="HOI51530.1"
                     /translation="ELTPNGEIAGWLEEERRSAGLSLERVPLDQLENWAFTENGRIAH
                     RSGRFFAIQGIEVSTDWGEVPNWGQPIIDQPEVGFLGLIAREFGGVLHFLMQAKVEPG
                     NLNAVQISPTLQATKSNYSRAHGGNAPRYLEYFIDARPDEILLDQLQSEQGARFLAKR
                     NRNIIIEPQGELALTPSFRWLTLGQIKHLLRQPNLINMDTRTVISGISYGEFDDRAEY
                     YFDLFARHFSSSNLPAISLLRSFLHAGDSLHSMEEILSWVTHLRSRYELHITRKPLAE
                     LRQWRIERDCIRHETNRFFKVIGTRIELHGREVRSWCQPLIESAQEGLLAFIIKPING
                     VYHFLVQAKLEPGNFDIIEFAPTVQCLTGNFRDNEPGQRPPYLDYVLSAAPAQRLVDT
                     MQSEEGGRFFQEQNRNLLVLADDEFPLATSENYRWMTLNQIKQFIRFNNYLNIQARSL
                     ISAISFA"
     gene            1383..2369
                     /locus_tag="PLN02_04080"
     CDS             1383..2369
                     /locus_tag="PLN02_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOI51531.1"
                     /translation="MSIRFGILGCASIARRMFAPAIAQLEEATLVAVASRDPARAAQF
                     AADFGCAAASDYHALLARDDIDAVYLPLPPALHETWAAHALAAGKHVLCEKPLTTDLA
                     ATSRLVEAARRAGKVLEENWMFLHHRQLQFIKDRLASGQLGEIRLLQASFGFPPLPPD
                     NFRYRAALGGGALLDAGGYTLRIARELLGDPLTLLGSHLQTPPDSEVDRFGSALLLSS
                     RGITAQLAFGFDSHYRCELEIWTANARLTAPRIFTAPPGFAPPVRIETGQGGEEHTIA
                     ADNHFANALRHFIRRIARHDTACDGLLRQATLTEALRTHARHPCHQTGPGAA"
     gene            2326..3441
                     /locus_tag="PLN02_04085"
     CDS             2326..3441
                     /locus_tag="PLN02_04085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOI51532.1"
                     /translation="MPAIHVTKPALAPLDAFARKLESAWESGVLTHNGPLVRELEARL
                     CDYLALPRLAAVTNGTIALQLAIRALGLRGEIITTPFSWIATVSAIVYEGCRPVFVDI
                     DPETLNIDPAQIEARITRHTSAILPVHVFSNPCATGEIGEIAARHRLKVIYDGAHAFN
                     VRQGGQPLALAGDVTALSFHATKLFNTGEGGACVTADPELDARIRRLRFFGHDDTRNI
                     VDDGTNGKMTEIHAALGLVNLEFAAAVERKRRSDYTHYLACLGSAPALRFQRIDPEAC
                     NHSYLPVIFPDEDSLLAAEQALLAESVVARRYFYPALNTLDRLFPYQPFPVAESISRR
                     ILCLPLYFELGTADIERISGIVVDSLARHSSAKRATP"
     gene            3438..4109
                     /locus_tag="PLN02_04090"
     CDS             3438..4109
                     /locus_tag="PLN02_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOI51533.1"
                     /translation="MSLPHLFIYGAGAFGKEIRDIARRANRQEQTWDKIFFIDDFAPP
                     GTWFGDCQSFPFTSVLEDFDSAAIEVVIAQGEPVHRETLYGKLSQCGIRLSRVVDPSA
                     IVSPYASVGEGVVLTAFCSVAADAKVGNNVAVNVQAIIGHDVVVGDHTVISSMVNLGG
                     GTRVGTATYIGMGSQVKEQLRIGDRSIVGMGSVVHHDLGDDLIALGNPARAMRKNETR
                     WVFNK"
BASE COUNT          777 a         1579 c         1180 g          672 t
ORIGIN      
        1 cgaactgacg cccaacggcg aaatcgccgg ctggctggaa gaagaacgtc gctcggccgg
       61 actgtcgctc gaacgcgtcc ccctggacca gctggaaaac tgggcgttca ccgaaaacgg
      121 tcgcatcgcc caccgcagcg gccgcttctt cgcgatccag ggcatcgagg tgagcaccga
      181 ctggggcgag gtcccgaact ggggccagcc gatcatcgac caaccggaag tcggcttcct
      241 cggcctgatc gcccgtgaat tcggcggcgt cctgcatttc ctgatgcagg ccaaggtcga
      301 gcccggcaat ctcaacgcgg tccagatctc gccgaccctg caggcgacca agagcaatta
      361 ttcgcgcgcc catggcggca acgcgccgcg ctacctggaa tacttcatcg acgcccggcc
      421 cgacgaaatc ctgctcgatc agttgcagtc cgagcagggc gcccgcttcc tggccaagcg
      481 caaccgcaac atcatcatcg aaccccaggg cgaactggcg ctgaccccca gcttccgctg
      541 gctgacgctg ggccagatca agcacctgct gcgccagccg aacctcatca acatggatac
      601 ccggaccgtc atttccggga tttcctacgg cgagttcgac gatcgcgccg agtactactt
      661 cgacctcttc gcgcgccact tctcaagcag caacctgccc gccatttcgc tcctcaggtc
      721 attcctccac gccggcgaca gcctgcacag catggaggaa atcctctcct gggtgacgca
      781 cctgcgctcg cgctacgaac tgcacatcac ccgcaagccg cttgccgaac tgcgccagtg
      841 gcggatcgag cgggattgca tacgccacga aaccaaccgc ttcttcaagg tgatcggcac
      901 ccgcatcgag ctgcacggcc gggaagtgcg cagctggtgc cagccgctca tcgagagcgc
      961 ccaggaaggc ctgctcgcct tcatcatcaa gccgatcaac ggcgtctatc acttcttggt
     1021 ccaggccaaa ctggagccgg gcaatttcga catcatcgag ttcgcgccga ccgtccagtg
     1081 cctgaccggc aatttccgcg acaacgaacc cgggcagcgg ccgccctatc tcgactacgt
     1141 gctgtccgcg gcacccgcgc agcgcctggt cgacaccatg cagtccgaag aaggcgggcg
     1201 tttcttccag gaacagaacc gcaacctgct ggttctggcg gacgacgaat tcccgctggc
     1261 gacctcggaa aactaccgct ggatgaccct gaaccagatc aagcagttca tccgcttcaa
     1321 caattacctg aacatccagg cacgcagcct gatttccgcc atttccttcg cgtgatcaag
     1381 gcatgagcat ccgcttcggc atcctcggct gcgccagcat cgcccgccgc atgttcgcgc
     1441 cggccatcgc ccagctcgag gaggcaacat tggtcgccgt cgcttcccgc gatcccgctc
     1501 gcgccgcgca gttcgccgcc gatttcggct gcgcggcggc cagcgactac cacgccctgc
     1561 tcgcccgcga cgacatcgac gccgtctatc tgccgctgcc gccggccctg cacgaaacct
     1621 gggccgctca cgcgctggcg gcgggcaagc acgtcctctg cgagaagccg ctgaccaccg
     1681 acctcgccgc aaccagccgc ctggtcgagg cggcgcgccg cgccggcaag gtcctggagg
     1741 aaaactggat gttcctccac caccgccaac tgcagttcat caaggaccgg ctcgccagcg
     1801 gccaactggg cgaaatccgc ctgctccagg ccagcttcgg cttcccgccg ctgccgccgg
     1861 acaacttccg ttaccgcgcg gcgctgggcg gcggcgcact gctcgatgcc ggcggttaca
     1921 ccctgcgcat cgcccgcgaa ctgctcggcg acccgctgac gctgctcggc agccacctgc
     1981 agacgccgcc ggacagcgag gtcgaccgct tcggcagcgc cctgctgcta tcgtcccgag
     2041 gcatcaccgc ccagctcgcc ttcggtttcg acagccacta ccgctgcgag ctggaaatct
     2101 ggaccgccaa cgccagactg accgccccca gaatcttcac cgcaccgccc ggtttcgccc
     2161 cgccggtccg gatcgaaacc ggacaaggcg gcgaagaaca cacgatcgcc gccgacaacc
     2221 actttgccaa cgccctgcgc cacttcatcc gccgcatcgc ccgccacgac acggcgtgcg
     2281 acggactgct gcgccaggcc acgctcaccg aagccctgag aacccatgcc cgccatccat
     2341 gtcaccaaac cggccctggc gccgcttgac gctttcgccc gcaagctcga gtccgcctgg
     2401 gaaagcggcg tcctgacgca caacggcccg ctcgttcgcg aactcgaagc gcggctctgc
     2461 gactacctgg cgctgccccg gctcgccgcc gtcaccaacg ggaccatcgc cctgcaactg
     2521 gcgatccgcg ccctcggcct gcgcggcgaa atcatcacca cgcccttttc ctggatcgcc
     2581 accgtcagcg ccatcgtcta tgaaggctgc cggccggtct tcgtcgacat cgacccggaa
     2641 acgctgaaca tcgacccggc gcagatcgaa gcccgcatca cccgccacac cagcgccatc
     2701 ctgcccgtcc atgtcttcag caacccatgc gccaccgggg agatcggcga aatcgccgcc
     2761 cgccaccggc tcaaggtcat ctacgacggc gcccacgcct tcaacgtcag gcagggcggc
     2821 caaccgctgg cgctggccgg cgacgtcacc gccctcagct tccacgcgac caagctgttc
     2881 aacaccggcg agggcggcgc ctgcgtcaca gccgacccgg aactagacgc gcgcatccgc
     2941 cgcctgcgct tcttcggcca cgacgacacg cgcaacatcg tcgacgacgg caccaacggc
     3001 aagatgaccg agatccatgc cgcgctgggc ctggtcaacc tcgaattcgc cgcggccgtc
     3061 gaacgcaagc ggcgtagcga ttacacgcac tacctggcct gtctcggcag cgcgccggca
     3121 ctgcgcttcc agcgcatcga cccggaagcc tgcaaccaca gctacctgcc ggtcattttt
     3181 ccggacgagg acagcctgct cgccgccgag caggcgctgc tcgccgaatc tgtcgtcgcc
     3241 cggcgctact tctacccggc gctcaacacc ctcgaccgtc tctttcccta ccagcccttc
     3301 ccggtcgccg aaagcatctc ccggcgcatc ctctgcctgc cgctgtattt cgagctcggc
     3361 accgccgaca tcgaacggat cagcggcatc gtcgtcgatt cgctggcccg gcattcctcg
     3421 gcaaagcgcg caacaccatg agccttcccc atctcttcat ctacggcgcc ggcgccttcg
     3481 gcaaggaaat ccgcgatatc gcgcgacgcg ccaaccggca ggaacagacc tgggacaaga
     3541 tcttcttcat cgacgatttc gcaccgccgg gcacctggtt cggcgactgc cagtccttcc
     3601 cgttcacctc ggtgctggaa gatttcgaca gcgccgccat cgaggtggtg atcgcccagg
     3661 gcgaaccggt gcaccgcgag accctgtacg gcaaactgag ccaatgcggc atccgcctga
     3721 gccgggtcgt cgacccctcg gcaatcgtct cgccctatgc cagcgtcggc gaaggcgttg
     3781 tgctgacggc cttctgcagc gtcgccgcgg atgctaaagt cggtaataat gtcgccgtta
     3841 atgtccaggc catcatcggt cacgacgtcg tcgtcggcga ccatacggtg atctcgtcga
     3901 tggtcaatct gggcggcggc acccgcgtcg ggaccgccac ctatatcggc atggggtcgc
     3961 aggtgaagga acaactgcgc atcggcgacc gcagcatcgt cggcatgggg tcggtcgtcc
     4021 accatgacct cggcgacgac ctgatcgccc tgggcaatcc ggcgcgggcc atgcgaaaaa
     4081 acgaaacccg ctgggttttc aacaagtaag gaaaggcact cgctcgatga gcaatcagga
     4141 aaaataccag caggtcttca tcgaatgctt cggcgtcgcc gccgacgtcc tcggtgacca
     4201 gtttacct
//
LOCUS       DAOSDX010000135         4201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7446_length_4201_cov_2.910034, whole genome shotgun sequence.
ACCESSION   DAOSDX010000135 DAOSDX010000000
VERSION     DAOSDX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7446_length_4201_cov_2.910034"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..637)
                     /locus_tag="PLN02_04095"
     CDS             complement(194..637)
                     /locus_tag="PLN02_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOI51534.1"
                     /translation="MFKNILVPTDGSELSRATAKRAVAFASEAGARVTAFYAKPEYPI
                     AYFGEGALIDPTTPEKFTQMAEQQAAEYLNEVAAWCQEAKVECTTASATSDIPYEAII
                     EAAEKAGCDLIFMASHGRRGISGLLLGSETNKVLTHSKIPVLVYR"
     gene            721..2133
                     /locus_tag="PLN02_04100"
     CDS             721..2133
                     /locus_tag="PLN02_04100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI51535.1"
                     /translation="MRLGFFGAAGEVTGSCTLVDTGEARFLVDCGMFQGGAEARAKNE
                     RALSFGFDVRRLDFVLLTHAHIDHSGLLPRLAMLGFRGPVYTTPATISLLEVLLPDAA
                     HIQEKEAEWQLRNRHRRGRSERGILPPLYTVMQAQAALKLLRPVPYDESFAPLAGIGV
                     RFRDAGHILGSASLEVTVASGGHSRRLVFSGDLGMPGRPVLNDPAPAPASADALLIES
                     TYGNRLHRSLAETEEEIVNAFARTRAAGGNIVIPAFAVGRTQEILFILTDLVRRGRLP
                     AMKVFVDSPMANSATRITLEHSHLLDAPTRELIAWQQAHPRKMDIEFVADVERSRQLN
                     DIRHGAVIISASGMCDAGRIKYHLRENLGRHESTVLIAGFQAAGTLGRRLVDGAKLVR
                     IFGETVSVKAKICTVGGLSAHADQAALLDWLAGFHKAPGKLFVVHGESSASQAFADAI
                     RARFGWREVHLPQANSFVTL"
     gene            2165..3913
                     /locus_tag="PLN02_04105"
     CDS             2165..3913
                     /locus_tag="PLN02_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI51536.1"
                     /translation="MSGNPATQERSIDLDGLSPVETVGKAVSKAIDPFGATTSLLNAQ
                     MAWLMHPQELTRAVNALSADLFALQAHVARRALGIASADVIQPHADDARFTAPVWSDS
                     ASWDIVKEWYLAFTHRLQDMLFETPGMSDKERRRAAFWSRKWLNMVAPTNFFWTNPVA
                     IERAVATQGDSLRRGWANFQRDVKAKNIQMVEPDAFTVGKDLATTPGKVVFRNRLVEL
                     IHYTPTTEKVRAMPIVIVTPWINKFYILDLNARKSLVKYLTDQGFSVFITSWKNPDAE
                     MHDVRFDDYLLEGVNEVVRVASEFCKVPQVHLVGYCIGGTLVSTYMAWANKRFAAEQM
                     PVAHWTLFTTLTDFAHPGDIDVFIDDACVGALEEKMAKRGYLDGAEMATSFRLLRSNS
                     LVWQYWVNSYLLGEPLPPFDVLFWNCDTTRMPAAMHSYYLREMYLNNNLIKRGKLSIA
                     GEPIDLDRIVQPLYAVTAEDDHIAPWKQCYRIRKYINVQAPMRFALSTSGHILGIVNP
                     PVNPPKRAYWIAEPERNEHWEHWLDRADKKPGTWWQDWTAWLGERTGELVDAYPVANR
                     KFPALADAPGTYVLEK"
     gene            3910..>4201
                     /locus_tag="PLN02_04110"
     CDS             3910..>4201
                     /locus_tag="PLN02_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOI51537.1"
                     /translation="MTALVRTAGAAAGEDLRRIVRINEEVKAVVAVAFRINLMALNAI
                     FLAKRAGINARGFGVLSNELRVFSQDLRTQMQTLTAHIHAGVRDVSVVLQDIR"
BASE COUNT          743 a         1409 c         1335 g          714 t
ORIGIN      
        1 cgcgcaggtg cagggcgaag gccgcgacga aaatcgccac caggccgagc agggcaaggc
       61 tgaccgggac gaggatgcgg cgacgaaaag ggatgctggg ctggagggaa ggcatcaacg
      121 acccgcccgc gggcggggca acggtagctt gccggaaggc ccgccggcac cgacgcggac
      181 gggccgggga aggtcagcga tagaccagga ccggaatctt ggaatgggtc agtaccttgt
      241 tggtttcgct gcccagaagc aggccactga tcccgcggcg gccgtgcgaa gccatgaaga
      301 tcaggtcgca gccggccttc tcggcggctt cgatgatcgc ttcgtaggga atgtcgctgg
      361 tcgccgaggc ggtggtgcat tcgaccttgg cttcctggca ccaggcagcg acttcgttca
      421 ggtattcggc ggcttgctgt tcggccatct gggtgaattt ttccggcgtc gtcgggtcga
      481 tcaaggcgcc ttcgccgaaa taggcgatcg gatattcagg cttggcgtag aacgcggtaa
      541 cgcgggcgcc ggcttcgctg gcaaaggcta ccgcgcgctt ggcggtggca cgggaaagtt
      601 ccgaaccgtc ggtgggaacc agaatgttct tgaacatgct atacctcctg aaatggtctg
      661 ctggcgttgc tttccgccgt ttatgggttc atgctacgcc aagtcgggag gttttcaaac
      721 atgcgacttg gctttttcgg tgccgctggc gaagtgaccg gttcctgcac cctggtcgat
      781 accggggaag cgcgttttct ggtcgattgc ggcatgtttc agggcggcgc cgaggcacgc
      841 gccaagaacg aacgggcact gtccttcggc ttcgacgtgc ggcgcctcga cttcgtgctc
      901 ctcacccatg cccacatcga tcattccggc ctcttgccgc gcttggcgat gctcggcttc
      961 cgcggcccgg tgtataccac gccggcgacc atctcgctgc tcgaagtgct gctgccggac
     1021 gccgcgcata tccaggaaaa ggaagccgag tggcaactgc gcaaccgcca tcgccggggg
     1081 cgcagcgagc gcggcatcct gccgccgcta tacacggtga tgcaggccca ggcggcgctc
     1141 aagctgctgc gcccggtgcc ctacgatgaa agcttcgccc cgctggccgg gatcggcgtg
     1201 cgcttccgcg atgccggcca catcctcggc tcggcctcgc tcgaagtgac cgtcgccagc
     1261 ggcggccaca gccgccggct ggtcttctca ggcgacctcg gcatgcccgg ccggccggtg
     1321 ctcaacgacc cggcgccagc gccggcaagc gccgacgcgc tgctcatcga atcgacctac
     1381 ggcaaccgcc tgcatcgctc gctcgccgaa accgaggaag agatcgtcaa cgccttcgcg
     1441 cgcacccgcg ccgccggcgg caacatcgtc attccggcct tcgccgtcgg gcgtacccag
     1501 gagattctct tcatcctcac cgacctggtg cggcgcggcc gcttgccggc gatgaaggtc
     1561 ttcgtcgatt cgccgatggc caattcggcc acccgcatca cgctcgaaca cagccacctg
     1621 ctcgatgccc cgacccgcga actgatcgcc tggcagcagg cgcatccgcg caagatggac
     1681 atcgagttcg tcgccgacgt cgaacgttcg cgccagttga acgacatccg ccacggcgcg
     1741 gtgatcatct cggcgagcgg catgtgcgac gccggacgga tcaagtacca cctgcgcgag
     1801 aacctgggcc gtcacgaatc gacggtgttg atcgccggtt tccaggcggc cggtacgctc
     1861 ggtcgacgcc tggtcgacgg cgcgaagctg gtccgcatct tcggcgaaac ggtgtcggtc
     1921 aaggcgaaga tctgcacggt cggcggcctt tccgcgcatg ccgaccaggc ggcgctgctc
     1981 gactggctgg ccggttttca caaggcgccc ggcaagctgt tcgtggtgca tggcgaaagt
     2041 tcggcgtcgc aggccttcgc tgacgccatc cgcgcccgtt tcggctggcg ggaggtgcat
     2101 ctgccgcagg cgaacagctt cgtcacgctg taagatcgaa tctgaagata tcagggagaa
     2161 tcccatgtca ggcaatccgg caacacaaga acgcagtatc gacctcgacg ggttgagccc
     2221 ggtcgagacg gtcggcaagg cagtgagcaa ggcgatcgat cccttcgggg cgaccacctc
     2281 gctgctcaac gcgcagatgg cgtggctgat gcatccgcag gaactgacgc gcgcggtcaa
     2341 cgcgctctcc gccgacctgt tcgcgttgca ggcgcacgtt gcccggcggg cgctcggcat
     2401 tgcgtccgcc gacgtgatcc agccgcatgc cgacgacgcc cgcttcacgg cgccggtatg
     2461 gagcgattcg gcgtcctggg acatcgtcaa ggagtggtat ctcgccttca cccaccgtct
     2521 gcaggacatg ctcttcgaaa cccccggcat gtcggacaag gaacggcggc gggcggcgtt
     2581 ctggtcgcgc aagtggctga acatggtggc gccgaccaat ttcttctgga ccaacccggt
     2641 cgccatcgag cgcgccgtcg ccactcaggg cgacagcctg cgccgtggct gggccaactt
     2701 tcagcgcgac gtcaaggcga agaacatcca gatggtcgag cccgacgcct tcaccgtcgg
     2761 caaggacctg gcgacgacgc cgggcaaggt ggttttccgc aaccgtctgg tcgagctgat
     2821 tcactacacg ccgaccaccg agaaggtgcg ggcgatgccc atcgtcatcg tcacgccgtg
     2881 gatcaacaag ttctacatcc tcgacctgaa cgccaggaag agcctggtca agtacctcac
     2941 cgaccagggt ttttcggttt tcatcaccag ctggaagaac ccggacgccg agatgcacga
     3001 cgtccgtttc gacgattacc tgctcgaagg cgtcaatgaa gtggtgcgcg tcgccagcga
     3061 attctgcaag gtgccgcagg tccatctggt cggctactgc atcggcggca ccctggtcag
     3121 cacctacatg gcctgggcca acaagcgttt cgccgccgag cagatgccgg tcgcccactg
     3181 gacgctgttc accacgctga ccgacttcgc gcaccccggc gacatcgacg tcttcatcga
     3241 cgacgcctgc gtcggcgcgc tggaagagaa gatggccaag cgcggctatc tcgacggcgc
     3301 cgaaatggcc acctcgttcc gcctgctgcg ttcgaacagc ctggtctggc agtactgggt
     3361 caacagctac ctgctcggcg aaccgctgcc gcctttcgac gtgctgttct ggaactgcga
     3421 tacgacgcgc atgccggcgg cgatgcattc ctactacctg cgcgagatgt acctcaacaa
     3481 caacctgatc aagcgcggca agctgagcat cgccggcgag ccgatcgacc tcgatcgcat
     3541 cgtccagccg ctctacgcgg tgaccgccga ggacgaccac atcgcgccgt ggaagcagtg
     3601 ctaccgcatc cgcaagtaca tcaacgtcca ggcgccgatg cgcttcgcgc tgtccacctc
     3661 cggacacatc ctcggcatcg tcaatccgcc ggtcaatccg cccaagcgcg cctactggat
     3721 cgccgaaccg gaacgcaacg agcactggga acactggctt gaccgcgccg acaagaagcc
     3781 cggcacctgg tggcaggact ggaccgcctg gctcggcgag cgtaccggcg aactggtcga
     3841 cgcctacccg gtggccaacc gcaaattccc ggcgctggcc gacgcgcccg gcacctacgt
     3901 gctcgagaaa tgacggcgct ggtgcggacg gccggtgccg cagccggcga agacctgcgg
     3961 cgcatcgtcc gcatcaacga ggaggtcaag gccgtcgtcg ccgttgcctt tcgcatcaac
     4021 ctgatggcgc tcaacgccat cttcctggcc aagcgcgccg gcatcaacgc gcgcggcttc
     4081 ggcgtcctct ccaacgagtt gcgcgtcttc tcgcaggacc tgcggacgca gatgcagacg
     4141 ctgaccgcac acatccacgc cggcgtgcgc gacgtctcgg tcgtcctcca ggacatccgc
     4201 a
//
LOCUS       DAOSDX010000136         4162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7548_length_4162_cov_2.259800, whole genome shotgun sequence.
ACCESSION   DAOSDX010000136 DAOSDX010000000
VERSION     DAOSDX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7548_length_4162_cov_2.259800"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLN02_04115"
     CDS             <1..223
                     /locus_tag="PLN02_04115"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HOI51538.1"
                     /translation="GTGAGMEPPPDAVYRDGAWHYAPQLPVLPRVALRHSPHVAPYVV
                     CAGGRCAEIGQWLPGLAEDAVVELAPCAP"
     gene            complement(368..452)
                     /locus_tag="PLN02_04120"
     tRNA            complement(368..452)
                     /locus_tag="PLN02_04120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(416..418),aa:Leu,seq:cag)
     gene            493..2724
                     /gene="rnr"
                     /locus_tag="PLN02_04125"
     CDS             493..2724
                     /gene="rnr"
                     /locus_tag="PLN02_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOI51539.1"
                     /translation="MSRKKISKIRRADPFYEREAQRYEFPLPSREYIAQVLADEGRPL
                     SFDELCGLLDIAKDEREMFQRRLGAMEREAQLMRNRKGDYILPERASLTAGRIQGHQD
                     GYGFLIPDDGSADIFLDQRQMGKVLHGDRALVRVTGLDRKGRPEGSIVEVTERANSRV
                     VGRVFVEHGVVFVVPENKRIAQDILVPPEKKSKLKAQSGQVVMVEIIEQPSKHSQPIG
                     RIVEVLGNYADPGMEIEIALRKHDLPFEFPKAALEENKKMPDKVRKTDLKGREDLREL
                     PLVTIDGETARDFDDAVFCEKQGRGWRLVVAIADVSHYVQPGMALDREALARGNSVYF
                     PRRVIPMLPEKLSNGICSLNPDVERLAMVCDVAISAAGKIGKYRFYPAVFRSHARLTY
                     NQVWSWLSGEKTPETEAQRNVLPQVQNLYKLYQALHKARGQRGAIDFETTETMMLFND
                     QGKIENIVPVVRNDAHRIIEECMLAANVCASDFLAQHKQTCLYRIHEAPSEEKLRNLR
                     SFLGEFGLTLGGGDDPRAKDYALLLEKIKPRPDFQLLQTVMLRSLKQAMYSPDNVGHF
                     GLAYEHYTHFTSPIRRYPDLLVHRAIKAVLSGGKYEPGQAWDEIGLQCSATERRADEA
                     TRDVENWLKTFFMKERIGEVFDGTISAVTAFGIFVALDQVYVEGLVHVSELGQDYFQF
                     DNVKHQMLGERTGERFRLGDRVRVKLVRADLESNKIDFVLVREERAVAAGSKGKRNMA
                     RKR"
     gene            2803..3312
                     /locus_tag="PLN02_04130"
     CDS             2803..3312
                     /locus_tag="PLN02_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOI51540.1"
                     /translation="MLTKRLGIDPLAGELPASLETGHVRIDRDHRQLLALLGELRRIC
                     RDPGGLPDCATCSADQQLACEGRLIVLLGDLFSCILEHFAGEELLMRDAQMQLAEGEH
                     YAAHIEDHAAIAQKVQELVARLDIVHTVERIRELDGLLARWVANHIEMHDDLLVRWLA
                     KRGIPGAAV"
     gene            3358..4089
                     /gene="rlmB"
                     /locus_tag="PLN02_04135"
     CDS             3358..4089
                     /gene="rlmB"
                     /locus_tag="PLN02_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOI51541.1"
                     /translation="MSSRLIYGFHAITAKLRQDPESLREILIDGDRQDARARDLLRHA
                     ELQGVRVILADGKRLDDLAGGGRHQGVVARLDGERKAAHLDDVLDTLEEPAFLLVLDG
                     VTDPRNLGACLRVADAAGVHAVIAPKDRSAGLNDVAIKTACGAAESVPYVMVTNLART
                     LRELKEREIWVVGTAGEAAQDLYAAEWPQATAWVMGAEGEGMRRLTRENCDQLVSIPM
                     HGSVDSLNVSVAAGVCLFEARRRRG"
BASE COUNT          763 a         1305 c         1374 g          720 t
ORIGIN      
        1 cggcaccggc gccggcatgg agccgccgcc cgacgccgtc taccgggacg gtgcctggca
       61 ttacgcgccc caactgcccg tgctgccgcg cgtcgccctg cgccattcgc cccatgtggc
      121 gccctatgtc gtctgcgccg gcgggcgctg cgcggagatc ggccaatggc tgccgggcct
      181 ggcggaggat gcggtggttg aactggcgcc ctgcgcgcca tgaaggccgg cgcaccgtcc
      241 cgccggcgcc gagttttgct cctgaacctg gcgcggcata aaagccctac aatgccgccg
      301 tcgttcgtac tggcgaccgg gccggaaaca aaaaaggccc cgcgatgcgg agccttcttg
      361 cttcaactgg tgcccaggag aggactcgaa cctccacggt gttacccgct agtacctgaa
      421 actagtgcgt ctaccaattc cgccacctgg gcacaggtgc gaagagccgc cattataggg
      481 aagagaaaac cgatgtcaag aaaaaagatc agcaagattc gccgtgccga ccccttctac
      541 gagcgcgagg cacagcgcta cgaatttccc ctgccgtcgc gcgagtacat cgcgcaggtg
      601 ctggccgacg aggggcggcc gctgtccttc gatgaactgt gcggattgct cgacatcgcc
      661 aaggatgaac gcgagatgtt ccagcgccgg cttggggcga tggagcgcga ggcgcagctg
      721 atgcgtaacc gcaagggcga ctacatcctg cccgaacgcg cttcgctgac cgccgggcgt
      781 atccagggcc atcaggacgg ctacggcttc ctgatccccg acgacggtag cgccgacatc
      841 ttcctcgacc agcgccagat gggcaaggtg ctgcatggcg atcgcgccct ggtccgggtc
      901 accggcctcg atcgcaaggg gcgcccggaa ggcagcatcg tcgaggtcac cgaacgcgcc
      961 aacagccgtg tcgtcggccg cgtcttcgtc gagcacgggg tggtcttcgt cgttccggaa
     1021 aacaagcgca tcgcccagga catcctggtg ccgccggaaa agaagagcaa gctcaaggcg
     1081 cagtccggcc aggtggtgat ggtcgagatc atcgagcagc cgagcaagca ttcgcagccg
     1141 atcgggcgca tcgtcgaggt gctgggcaat tacgccgatc cgggaatgga gatcgagatt
     1201 gcgctgcgca agcacgatct gcccttcgaa ttcccgaagg cagcgctcga ggaaaacaag
     1261 aagatgcccg acaaggtgcg caagaccgac ctcaaggggc gcgaggactt gcgtgaattg
     1321 ccgctggtga cgatcgatgg cgagacggcg cgcgatttcg acgacgcggt tttttgcgag
     1381 aagcaggggc gcggttggcg gctggtcgtc gccatcgccg acgtttcgca ttacgtgcag
     1441 ccgggcatgg cgctcgaccg cgaagcgctg gcgcgcggca actccgtcta tttcccgcgc
     1501 cgcgtcatcc cgatgctgcc ggaaaagctg tccaatggca tctgttcgct gaatccggac
     1561 gtcgaacggc tggcgatggt ttgcgacgtg gcgatcagcg ccgccggcaa gatcggcaaa
     1621 taccgcttct atccggcggt cttccgttcg cacgcgcggc tgacctacaa ccaggtctgg
     1681 tcctggctga gtggcgagaa aacgccggaa accgaggcgc agcgcaacgt tttgccgcag
     1741 gtgcagaacc tctacaagct ctaccaggcg ctgcacaagg cgcgcggcca gcgcggggcg
     1801 atcgatttcg agaccaccga gacgatgatg ctgttcaacg accagggcaa gatcgagaac
     1861 atcgtgccgg tggtgcgcaa cgatgcccac cgcatcatcg aggaatgcat gctggcggcc
     1921 aacgtctgcg cctcggattt cctggcccag cacaagcaga cctgtctcta ccgcatccac
     1981 gaggcaccgt ccgaggaaaa actgaggaat ctgcgcagct tcctcggcga attcggtctc
     2041 accctgggtg gcggggacga tccgcgggcc aaggattacg cgctgctgct cgagaagatc
     2101 aagccgcggc cggatttcca gctactgcag acagtgatgc tgcgttcgct gaagcaggcg
     2161 atgtacagtc ccgacaatgt cggccatttc ggtctcgctt acgagcatta cacccatttc
     2221 acctcgccca tccggcgcta tccggacctg ctcgtccacc gggcgatcaa ggctgtgctc
     2281 agcggcggca aatacgaacc cggccaggcc tgggacgaga ttggcctgca gtgctcggcg
     2341 accgagcggc gtgccgacga ggcgacgcgc gatgtcgaga actggctgaa gaccttcttc
     2401 atgaaggaga ggatcggcga agtcttcgac ggcacgatct cggcggtcac cgccttcggc
     2461 atcttcgtgg cgctcgacca ggtctatgtc gagggcttgg tgcatgtctc ggagctgggg
     2521 caggactatt tccagttcga caacgtcaag caccagatgc tcggcgagcg taccggtgag
     2581 cgcttccggc tcggcgaccg ggtacgcgtc aagctggtgc gtgccgacct cgagtcgaac
     2641 aagattgatt tcgtgctggt tcgcgaggag cgggcagtgg ctgccggcag caagggcaag
     2701 cgcaacatgg cacgcaagcg ctgaatcgcc tccggtcccc agcctggttt agaatgaatg
     2761 ctcggggaat cgccaggaat aagaatgatg ggggaaacaa gcgtgctcac caagcggctc
     2821 gggatcgatc cgctcgcagg tgaactgccg gcttcgttgg aaacagggca tgtcaggatt
     2881 gatcgcgacc atcgccagtt gctggcgctg ctcggcgagt tgcggcgcat ctgccgcgat
     2941 ccgggtggct tgccggactg cgcgacatgt tcggcagatc agcagttggc ctgcgaggga
     3001 agattgatcg tgctgctcgg agatttgttc tcctgcatac ttgaacattt cgccggcgag
     3061 gaactgctta tgcgcgacgc gcagatgcag ctcgccgaag gcgagcacta cgcggcgcac
     3121 atcgaggatc acgcggcaat cgcccagaag gttcaggaac tggtcgcccg tctcgacatc
     3181 gtgcatacgg tggagcgcat tcgcgagctg gatggcttgc tggcccgttg ggttgccaat
     3241 cacatcgaaa tgcacgacga cctgctggtt cgctggctgg cgaagcgcgg cattccgggg
     3301 gctgcggtat gaaggtgggc ggggtaaaat gcgcctccct gtctgttcga gttgcccatg
     3361 tcttcccgcc tgatctacgg ctttcatgcc atcaccgcca agctgcgcca ggaccccgaa
     3421 tccctgcgtg aaatcctgat cgacggcgac cgccaggatg cgcgcgcccg cgatctgctg
     3481 cgccacgccg aactgcaggg ggtccgtgtc attcttgccg acggcaagcg gctcgacgat
     3541 ctggccggcg gtggccggca ccagggggtg gttgcccgtc tcgacggcga gcgcaaggcg
     3601 gcccacctcg acgacgtact cgatacgctg gaggagccgg cttttctgct cgtcctcgat
     3661 ggcgtcaccg atccgcgcaa tctcggcgcc tgcctgcgcg tcgccgacgc cgccggcgtc
     3721 catgcggtga tcgcgccgaa ggatcgctcc gccggcctca acgacgtcgc catcaagacc
     3781 gcctgtggcg ccgccgagag cgtaccctac gtgatggtta ccaacctggc gcggacgctg
     3841 cgcgaactca aggaacgcga aatctgggtg gtcggtaccg ctggcgaagc cgcgcaggac
     3901 ctttacgccg ccgagtggcc gcaggccacg gcctgggtga tgggcgccga aggcgagggc
     3961 atgcgccgtc tgacccggga aaactgcgac cagctggtca gcattccgat gcacggcagc
     4021 gtcgacagtc tcaacgtttc ggtcgccgcc ggcgtctgcc tgttcgaagc gcggcgccgc
     4081 cgcggttgag ttgatgtcgc atcgtcgcgc ggtctggctg atggtgctgg tgacgctcct
     4141 gtggagcatc gccggcgtcg tc
//
LOCUS       DAOSDX010000137         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7576_length_4152_cov_3.892848, whole genome shotgun sequence.
ACCESSION   DAOSDX010000137 DAOSDX010000000
VERSION     DAOSDX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7576_length_4152_cov_3.892848"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLN02_04140"
     CDS             complement(<1..427)
                     /locus_tag="PLN02_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOI51542.1"
                     /translation="MAALTLYLVRHPRPLIAPGVCYGQLDVAAEDPAPIVDRLRRELP
                     PGLPLWSSPLQRCRTLAEALQPAPRLDARLMELDFGAWEGRPWDDIARAELDAWAADV
                     DGYAPPGGEAPRQLQRRVLAFVDELPAGAHLLVTHAGAIR"
     gene            complement(415..1176)
                     /locus_tag="PLN02_04145"
     CDS             complement(415..1176)
                     /locus_tag="PLN02_04145"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOI51543.1"
                     /translation="MLRRELDYFLGALRFFTRLPVPAWVGHSAEALQHSARWLPAVGL
                     VVGGIAALVYLAALQIWPQPVAVLLAMAATLYATGAFHEDGLSDTADGLGGGWEKSRI
                     LAIMKDSRVGSYGVVALWLGLTGKFVLLAAFDPALVPAALLAGHAWSRGCATLLLASM
                     DYARDDDQSKARPLAQRLTGGALLFALAFALAPLLCLPWPQAVAGLLLALLATAWLAA
                     KFHRWLGGYTGDCLGATQQLAEIAFYLGLLAQWPR"
     gene            complement(1179..2222)
                     /gene="cobT"
                     /locus_tag="PLN02_04150"
     CDS             complement(1179..2222)
                     /gene="cobT"
                     /locus_tag="PLN02_04150"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOI51544.1"
                     /translation="MHSFAIVAPDRGIENSLRHKVDRKTKPPGSLGQLESLAVRIGLI
                     QQTLTPSLERPQMLVFAGDHGAARAGVSAYLQDVTWQMVENFLAGGAAINVFARQNGL
                     GLAVVDAGVAHDFGGPREGLIDLKVAPGTANYLETPAMTAAQCAQAIEQGAGVVFGLA
                     AAGCQVVGFGEMGIGNTASASLITHCLTGVPLADCVGRGTGLDDAGLARKQVLLEQAL
                     TRYRVAGGDDDPFAVLAEFGGFEMATMAGAMLAAAEAKMVLLIDGFIVGAAALVAARL
                     EPALLDYCVFCHHSAEPGHRAQLQALQAEPLLDLGLRLGEGTGAALAFPLVQAAVAFL
                     NEMASFESAGVAG"
     regulatory      complement(2294..2493)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2490..4061)
                     /locus_tag="PLN02_04155"
     CDS             complement(2490..4061)
                     /locus_tag="PLN02_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI51545.1"
                     /translation="MSLDTALKRSALYNPALWSRDEVRTYYVARPKLLARFIDDFRRE
                     KPGTRPQHRLILGQRGMGKSTLLRRLAIAVDDDEELAAAWIPLVFPEEQYNVGSLADF
                     WLNCLDALGDYLESHGRTAEMNAIDAEVERLDRKDGEGALQTLLRIAEKLGRRLILLV
                     DNIDLILDRLKDKDWALREALQEYPRLMLIGASSRALEASYDYGRAFYDFFRIDELRG
                     LSEDEMREIIVNLARRHGAEALIRRVQEDVGRMRVLHTLTGGNPRLAVLVYGVLSKGF
                     DSDVCADLESLLDEVTPQYKARFDEMPAQAQLLLDKLAVHWNPMSARQLADALGWSVN
                     QASAQLDRLAQAGIVEKVKAGQGKRMAFQVAERFFNIWYLMRAGRRARRRLMRLVGFL
                     HAYFSLDPGVVPDALRCAEPAPSQAAHIATADELMGEIRRLFAAQQEQVAAAPAYLRE
                     IVAMLPSAVELQVGERVLAELDASGIGESLQPVRAALVAAVAGNAELLNDVAPEIRLP
                     AREILARMAPSLVVG"
BASE COUNT          734 a         1432 c         1359 g          627 t
ORIGIN      
        1 cgcggatcgc gccggcgtgg gtgaccagca gatgcgctcc ggccggcagt tcatcgacga
       61 aggcgagcac gcgccgctgc aactggcgcg gcgcttcgcc gccgggcggc gcgtagccgt
      121 caacgtcggc ggcccaggcg tcgagttcgg cgcgcgcgat gtcgtcccac ggacgacctt
      181 cccaggcgcc gaaatccagt tccatcagcc gcgcatcgag ccgcggcgcc ggctgcaggg
      241 cttcggccag cgtccggcag cgctgcagcg ggctgctcca gagcggcaac cccggcggca
      301 gttcgcggcg caggcggtca acaatcggcg ccggatcttc ggccgcgaca tcgagctgac
      361 cgtaacagac gccgggcgcg atcagcggcc gcgggtggcg caccaggtac agcgtcagcg
      421 cggccattgc gcgagcagcc cgaggtagaa ggcaatttcg gcgagctgtt gggtggcacc
      481 caggcaatcg ccggtgtagc cgcccagcca gcggtggaac ttggccgcca gccaggccgt
      541 cgccagcagc gccagcagca ggccggccac cgcttgcggc caaggaaggc agagtaacgg
      601 cgccagcgcg aacgccaggg cgaacagcag cgccccccca gtcaggcgtt gggccagcgg
      661 cctggccttg gactgatcgt cgtcgcgcgc gtagtccatg ctcgccagca acagcgtcgc
      721 gcagccgcgc gaccaggcat gcccggcgag cagcgccgcc ggcaccagcg ccggatcgaa
      781 cgccgccagc aatacgaact tgccggtcag ccccagccac agcgcgacca cgccgtaact
      841 gccgacccgc gaatccttca tgatcgccag aatgcgcgac ttctcccaac cgccgccgag
      901 gccgtcggcg gtgtcggaca ggccgtcctc gtggaaggcg ccggtggcgt acagcgtcgc
      961 cgccatcgcc agcaacaccg ccaccggctg cggccagatc tggagcgccg ccaggtagac
     1021 caacgcggcg atgccgccga cgaccaggcc gaccgccggc agccagcgcg ccgaatgctg
     1081 cagtgcctcg gccgaatgac caacccaggc cggcaccggc agccgggtga agaaacgcag
     1141 ggcgccgagg aagtaatcga gctcgcggcg cagcatggct aaccggcgac gccggccgac
     1201 tcgaaactgg ccatttcgtt gaggaaggcg accgccgcct ggaccagcgg aaaagccagc
     1261 gcggcgccgg taccttcgcc caggcgcagg cccaggtcga gcagtggctc ggcctgcaag
     1321 gcctgcaact gggcacggtg gccgggctcg gccgaatggt ggcagaagac gcagtagtcg
     1381 agcaaggccg gttcgaggcg cgcggcgacc agcgccgccg cgccgacgat gaagccgtcg
     1441 atgagcagca ccatcttcgc ttccgccgcc gccagcatgg cgccggccat ggtcgccatc
     1501 tcgaagccgc cgaactcggc gagcacggca aaggggtcgt cgtcgccgcc tgcgacgcga
     1561 tagcgggtca gcgcctgctc cagcagcacc tgcttgcgcg ccagccccgc gtcgtcgagg
     1621 ccggtgccgc gcccgacgca atcggccagc ggcacgccgg tcaggcagtg ggtgatcaag
     1681 gacgccgagg cggtattgcc aatgcccatc tcgccgaaac cgaccacttg gcagcccgcg
     1741 gccgccagcc cgaagaccac gccggcgccc tgctcgatcg cctgcgcgca ttgcgctgcc
     1801 gtcatcgccg gggtttccag gtaattggcg gtacccggcg ccaccttgag atcgatcaga
     1861 ccttcgcgcg ggccgccgaa atcgtgcgcc acgccggcat cgaccaccgc caggccgaga
     1921 ccgttctggc gcgcaaagac gttgatcgcc gcgccgccgg cgaggaagtt ctcgaccatc
     1981 tgccaggtca cgtcctgcag ataggccgag acgccggccc tggcggcgcc gtgatcgccg
     2041 gcgaagacca gcatctgcgg ccgctcgagc gacggcgtca gcgtctgctg gatcaggccg
     2101 atgcgtaccg caagcgactc cagttgcccg agcgagccgg gcggcttggt cttgcggtcg
     2161 accttgtgtc tgagcgaatt ttcaatgccc cggtcggggg cgacgattgc gaaggaatgc
     2221 atccgattct ccaaaagcga aagggcctcg tgcccgcatc gtccagacgc gaacacgaag
     2281 ccctgtccac aggctttctg tttttccgtc cgacaggtct tctggctccc ggatcgtccc
     2341 cctgaccgcg ccttcccgga gcgtctgttt ccgctcgtag tggcttattg cggtggggtc
     2401 cccggttaca gcggcgggcc cgccccggaa ttgagttgtc tcgcaccggg ttccgttttg
     2461 tcgaacgcgg cgattatacc cgcgccacgc tagccgacga ccagcgacgg cgccatgcgg
     2521 gcgaggatct cccgcgccgg caagcggatt tcgggcgcca catcgttgag cagctcggcg
     2581 ttgccggcca ccgccgccac cagggcggca cgcaccggct gcagggactc gccaatgccg
     2641 gacgcgtcca gctcggccag gacgcgctcg ccgacctgca attccacggc agatggcagc
     2701 atggccacga tttcccgcaa atacgccgga gccgcagcga cttgctcttg ctgcgcggcg
     2761 aacaatcgac ggatttcgcc catcagttca tccgccgtag caatgtgcgc cgcttgagac
     2821 ggcgccggct cggcgcagcg cagcgcatcc ggaacgacgc ccggatcgag gctgaagtag
     2881 gcatgcagaa atccgaccag ccgcatcaac ctgcgccgcg cacgtcgacc agcgcgcatc
     2941 agataccaga tgttgaagaa acgttcggcg acctggaaag ccatgcgctt accctgccca
     3001 gccttgacct tttcaacgat gccagcttgc gccagacgat cgagctgggc cgaggcctgg
     3061 ttgacactcc agcccagggc atcggcaagc tgacgggcac tcatcgggtt ccagtgcacg
     3121 gccagcttgt cgagcagcag ttgcgcctgt gctggcattt cgtcgaaacg agccttgtac
     3181 tggggcgtca cttcatcgag cagactctcg agatcggcgc acacatcgct gtcgaatccc
     3241 ttcgacaaca cgccgtagac cagcacggcg agacgcgggt tgccgccagt cagcgtatgc
     3301 agcacgcgca tgcgcccgac atcctcctgg actcgcctga tcagcgcttc ggcaccgtgc
     3361 cggcgagcga ggttgacgat gatctcgcgc atttcgtctt cgctgagacc acgtagttcg
     3421 tcgatgcgga agaaatcata gaaagcgcgg ccgtagtcgt agctggcttc cagtgcccgg
     3481 ctgctggcgc cgatcagcat cagtcgcgga tattcctgca gggcttcgcg caaggcccaa
     3541 tccttatcct tgaggcgatc gagaatcaag tcgatgttgt cgacgagcag gatcagccgg
     3601 cgccccagct tttccgcgat acgcaacaag gtttgcaggg ctccttcgcc atccttgcgg
     3661 tcgagacgct cgacttctgc atcgatggcg ttcatttcgg cagtgcgacc gtggctttcc
     3721 aggtaatcgc ccagggcgtc gaggcagttg agccagaaat cggccagact gccgacgttg
     3781 tactgttctt ccgggaaaac caggggtatc caggcggcgg cgagttcctc gtcgtcatcg
     3841 acggcaatcg ccaggcggcg cagcagggtg gatttgccca tgccgcgttg gccgagaatc
     3901 agccggtgtt gggggcgggt acccggcttc tcgcgacgaa aatcgtcgat gaaccgggcg
     3961 agcagtttcg ggcgggcgac gtagtaggtg cgcacctcgt cgcggctcca caacgccggg
     4021 ttatagaggg cggaacgttt cagggcggta tccagactca tgctgccaac ctccgttgcc
     4081 agaaaacgcg cagccattcc agcctgaaag cgtagcgtcc ctcccgttca atcaggtagc
     4141 catcactggt ca
//
LOCUS       DAOSDX010000138         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7590_length_4145_cov_2.719560, whole genome shotgun sequence.
ACCESSION   DAOSDX010000138 DAOSDX010000000
VERSION     DAOSDX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7590_length_4145_cov_2.719560"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLN02_04160"
     CDS             complement(<1..636)
                     /locus_tag="PLN02_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOI51546.1"
                     /translation="MKRRAPSSATRLRPTAAGSFWLLAVLALLASAINYGNNLIFALA
                     FLLLALWLQTAWQCRRQLRRLVWQGNAPVAVFTGERLVVDGRVGNGQADAWVALADDG
                     RAGSPADFDAQGETTVALAIPTTQRGERQLATLRLVSRWPLGLWQASRPLPALRAIVY
                     PAPAGDLPLPASDPRHAHRQAASDDYHGLRAYAPGDPPRRINWRVYGRSGEL"
     gene            complement(633..1559)
                     /locus_tag="PLN02_04165"
     CDS             complement(633..1559)
                     /locus_tag="PLN02_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI51547.1"
                     /translation="MTHDDSASPALHAALAAVNQVLLGKSHAVRLAMSCLVARGHLLL
                     EDLPGMGKTVLAHALARTLGLAFNRVQFTNDLLPGDLTGAAIYVKEKEGFVFHPGPIF
                     TQLLLADEINRASPKTQSALLEAMEERQVSSDGTTRPLPEPFFVIATENPVDQLSTNA
                     LPEAQLDRFLMRVSLGYPDAAAELAVLRGDDRRELLAELPAVLDTAALLALQRQARAV
                     HASDALLAYVRALAEHTRHAAGLNYGLSPRAALGLLAAGRAWALLDGRDHVLPADIQA
                     VFPAVALHRLGLCDARQADAVATRFLEAVPLP"
     gene            complement(1576..3078)
                     /locus_tag="PLN02_04170"
     CDS             complement(1576..3078)
                     /locus_tag="PLN02_04170"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HOI51548.1"
                     /translation="MEKAIILSSAPRPRWLPLALVLLAAALGALGWAVWQTQFAWKSG
                     YGDGDLSHIAQADPRALSGGDLTTFHDGFMPFGQSADNLPWQYEAIFEEGDGVFDKTF
                     RPGDGRTASAVNSEGGMRRVGVGPLYNATGCAECHFRDGRVEQPYVSGGPMLGMFLRL
                     SVPDGKGGFKAPDGYHAQLRDKAVAGVPAEGIGHIDWEEIPGAFPDGEQFSLRRPHFR
                     IEAPAYGPLPENLIVEARTAPPVHGGGLIEAIADATLLSLVKTQENDPDGVSGKLSWL
                     TDPETGKKVSGRFSLKANEPSLRAQAAGAAFNDMGVTSPIHRRQMCLDNQTACLAAPH
                     GGTPEEPELPENLLRSLTVYLQLLAVPARRNLADPVALRGEQMFQAARCNACHVDTLQ
                     TGDTHEIARLRNQTIHPYSDFLLHDLGPDLAGRPDVLASASEWRTPPLWGIGLTERSN
                     HHTVFLHDQRARNLQEAILWHGGEASAAKARFMAMPKADRQALLKFLESL"
     gene            complement(3096..>4145)
                     /locus_tag="PLN02_04175"
     CDS             complement(3096..>4145)
                     /locus_tag="PLN02_04175"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF036558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOI51549.1"
                     /translation="RLALALLCLLPAGVAASTADPLAGASITLFAADADPSPAETTLT
                     LFGDDGPTTATLEDAPAADTGRGSLTLAAGYRHESLRFNIAADRSGKRTPNILSELEW
                     RVPMAELRLNADWTQRSGFTVEGRFAWAHGVSGGKVQDSDYLQNNRAGEFSRSYADPD
                     HSTAYDLSLGAGWRFPLGQRARLSATAGYALKHVHFSMRNGRQVLSLPPQSMPLGDFA
                     GLDSSYTPQWRGPWLALKGELALGAGFAVHAGLGYHRLKLKAEADWNLRDDRAHPVSF
                     RHSGSGDGWDAQLGARWRFSRGQELSLDLGKRRFDLKDGVHTNYFADGSASRLRLNEV
                     RSENWSAIVGYRVSY"
BASE COUNT          600 a         1332 c         1566 g          647 t
ORIGIN      
        1 cagttcgccg ctgcgcccgt agacgcgcca gttgatgcgg cgcggcggat cgcccggcgc
       61 gtaggcgcgc aggccatggt aatcgtcgct cgccgcctgg cggtgggcgt ggcgcggatc
      121 gctggcgggc agcggcaagt cgccagccgg ggctggatag acgatggcgc gcagcgccgg
      181 cagcggacgg ctcgcctgcc acagaccgag cggccagcgc gagacgagac gcagcgtggc
      241 gagctggcgt tcgccccgct gcgtggtcgg aatcgccagc gccaccgtcg tttcgccctg
      301 ggcgtcgaaa tcggccggcg atccggcacg cccgtcgtcg gccagcgcca cccaggcgtc
      361 ggcctggccg ttgccgaccc ggccgtcgac caccaggcgc tcgccggtaa acacggcgac
      421 cggcgcgttg ccctgccaaa ccaggcggcg caactggcgg cggcattgcc aggcagtctg
      481 cagccagagc gcgaggagca ggaaggcgag ggcgaagatc aggttgttgc cgtagttgat
      541 cgcgctcgcc agcagcgcca gcacggccag cagccagaag ctgccggcgg cggtcgggcg
      601 cagccgggtg gcggacgacg gggcgcggcg tttcatggca gcggtaccgc ttccaggaag
      661 cgggtcgcca ccgcgtcggc ctggcgcgcg tcgcacagcc ccaggcggtg cagggcgaca
      721 gccgggaaga cggcctggat gtcggccggc agcacgtggt cgcggccgtc gagcagcgcc
      781 caggcacggc cggcagcaag caggccgagc gcggcgcgcg gcgacaggcc gtaattgagc
      841 ccggcggcgt ggcgcgtgtg ttcggccagg gcgcgcacgt aggcgagcag cgcgtcgctg
      901 gcgtggacgg cgcgcgcctg gcgctgcagc gcgagcagcg ccgcagtgtc gagcaccgcc
      961 ggcagttcgg cgagcagttc gcgccgatcg tcgccgcgca gcaccgccag ttcggcggcg
     1021 gcgtccgggt agccgagcga gacgcgcatc aggaagcggt cgagctgcgc ttccggcagg
     1081 gcgttggtcg atagctggtc gaccgggttc tccgtcgcga tgacgaagaa gggttcgggc
     1141 agcgggcggg tcgtcccgtc gctcgacacc tgtctttcct ccatcgcttc gagcagcgcc
     1201 gactgcgtct tcggcgaggc gcggttgatc tcgtcggcga gcagcaactg ggtgaagatc
     1261 ggccccgggt ggaagacgaa gccttccttt tccttgacgt agatggcggc gccggtgagg
     1321 tcgccaggca gcaggtcgtt ggtgaactgg acgcggttga acgccaggcc cagcgtccgg
     1381 gccagcgcgt gggcgagcac ggtcttgccc atgccgggca ggtcttcgag cagcaggtgg
     1441 ccgcgcgcca cgaggcagct catggccagg cggacggcgt gggatttgcc gagcaatact
     1501 tggttgaccg cggccagcgc ggcgtgcagg gcaggggagg cggaatcgtc gtgggtcatg
     1561 ggaagtttcg cagggtcaga gggattcgag gaatttcagc agcgcctggc gatccgcctt
     1621 cggcatcgcc atgaaacgcg ccttggcggc cgaggcttcg ccgccgtgcc agaggatggc
     1681 ttcctgcagg ttgcgcgccc gctggtcgtg caggaagacg gtgtggtggt tgctgcgctc
     1741 ggtcaggccg atgccccaca gtggcggcgt gcgccattcg ctggcactgg ccagaacgtc
     1801 cgggcggccg gccaggtcgg gccccagatc gtggagcagg aaatcgctgt aggggtggat
     1861 cgtctgattg cgcaggcggg cgatctcgtg cgtgtcgccg gtttgcagcg tatcgacgtg
     1921 acaggcgttg cagcgtgccg cctggaacat ctgctcgccg cgcagggcga ccgggtcagc
     1981 gagattgcgc cgggccggca cggcgagcag ttgcagatag acggtcagcg agcgcagcag
     2041 gttctccggc agttccggtt cctccggcgt gccaccgtgc ggcgcggcaa ggcaggcggt
     2101 ctggttgtcc aggcacatct gccgacggtg gatcgggctg gtgacgccca tgtcgttgaa
     2161 cgcggcgccg gccgcctggg cgcgcaggct gggctcgttg gccttgagcg agaaacggcc
     2221 gctcaccttt tttccggttt ccgggtcggt cagccagctc agcttgccgg acacgccgtc
     2281 ggggtcgttt tcctgtgtct tgacgagaga cagcagcgtt gcgtcggcaa tcgcctcgat
     2341 caggccgccg ccatgcaccg gcggcgccgt ccgcgcttcg acgatcaggt tctccggcag
     2401 ggggccgtag gccggtgcct cgatgcggaa atgcggccgg cgcaggctga attgctcgcc
     2461 gtcgggaaac gcgccgggaa tctcttccca gtcgatatgg ccgatcccct ccgccgggac
     2521 gccagccacc gccttgtcgc gcaactgcgc gtggtaaccg tccggcgcct tgaagccgcc
     2581 cttgccgtcc ggcaccgaca ggcgcaggaa catgccgagc atcggcccgc cgctgacgta
     2641 gggttgttcg acccgaccgt cgcggaaatg gcattcggcg cagccggtgg cgttgtacag
     2701 cggcccgacg ccgacccggc gcatgccgcc ttcgctattg accgccgatg ccgtgcgccc
     2761 gtctccgggg cggaaagtct tgtcgaagac gccatcgcct tcctcgaaga tcgcttcgta
     2821 ctgccagggt aggttatcgg ccgactggcc gaagggcatg aagccgtcgt ggaaggtggt
     2881 caggtcgccg cccgacaagg cgcgcggatc ggcctgtgcg atatggctga ggtcgccgtc
     2941 gccatagccc gatttccagg cgaactgcgt ctgccacacc gcccagccga gtgcgccgag
     3001 ggcagcggcg agcagcacga gggcgagggg gagccagcgc ggacggggtg cgctgctcag
     3061 aatgatggct ttttccacgt gcttttcact cccgctcaat aagagactcg atagccgacg
     3121 atggccgacc agttttcgga gcgcacctcg ttgaggcgta ggcggctggc gctgccgtcg
     3181 gcgaagtaat tggtgtgcac tccgtctttc agatcgaagc ggcgttttcc gagatcgagg
     3241 ctgagctcct ggccgcgcga aaaccgccag cgggcgccca gttgcgcgtc ccagccgtcg
     3301 ccgctgccgg agtggcgaaa gctcaccggg tgggcgcgat cgtcgcgcag gttccagtcc
     3361 gcttcggcct tcagcttcaa gcggtgatag ccgagcccgg cgtgtacggc aaagccggcg
     3421 ccgagcgcca gttcgccctt gagggcgagc caggggccgc gccattgcgg ggtgtagctg
     3481 ctgtcgaggc cggcgaagtc gcccagcggc atgctctggg gaggcaggct gagtacctgg
     3541 cgaccgttgc gcatgctgaa gtggacatgt ttgagcgcgt aaccggcggt tgcactcagg
     3601 cgagcccgtt gcccgagcgg gaaacgccag cctgcgccca gcgacaggtc gtaggccgtg
     3661 ctgtggtcgg gatcggcgta ggatcgggag aactcgccgg cccggttgtt ctgcaggtag
     3721 tcggaatcct ggaccttgcc gccgctgacg ccgtgcgccc aggcgaagcg gccttcgacg
     3781 gtgaagccgc tgcgctgggt ccagtcggcg ttcaggcgca gttccgccat cggcacccgc
     3841 cattcgagtt cggacaggat gttcggcgtt cgcttgccgc tgcgatcggc ggcgatattg
     3901 aaacgcaggc tttcgtgccg gtagccggca gccagcgtca ggctgccgcg tccggtgtcc
     3961 gccgccgggg cgtcctcaag ggtggcggtc gtcgggccat cgtcgccgaa cagggtcagc
     4021 gtcgtttcgg cgggcgaggg atcggcgtcg gcggcgaaca atgtgatgct cgcgcccgcc
     4081 agcgggtcgg cggtgctggc ggcgacgccg gccggcagca ggcagaggag cgccagggcg
     4141 agcct
//
LOCUS       DAOSDX010000139         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7597_length_4142_cov_2.353315, whole genome shotgun sequence.
ACCESSION   DAOSDX010000139 DAOSDX010000000
VERSION     DAOSDX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7597_length_4142_cov_2.353315"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLN02_04180"
     CDS             complement(<1..184)
                     /locus_tag="PLN02_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51550.1"
                     /translation="MKRDETPLMTPEQAAEARSIGEKLARLRLARRIRQADAAARAGL
                     SRSTAALIEKGDPGRTL"
     gene            complement(324..1757)
                     /locus_tag="PLN02_04185"
     CDS             complement(324..1757)
                     /locus_tag="PLN02_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HOI51551.1"
                     /translation="MNNIKPAEYSERLYNEDLAALKDKNWNWYNIFAFWMSDVHSVGG
                     YVFAGSLFALGLGSWQVLVSLLVGIVLVNILCNLVARPSQMAGIPYPVACRMSFGIWG
                     ANIPAIIRGLIAVAWYGIQTFLASSALSIVLLRFFPGLESLTHSQFLGLSYLGWVGFM
                     TMWVLQAAVFWHGMEAIKRFIDWAGPAVYVVMFALAAWIVWKAGPENISLNLGEVKYS
                     GLESLGMMITAIALVVSYFSGPMLNFGDFSRYGRTMEEVKRGNFWGLPVNFLAFSVVT
                     VVITSGTIPVFGHMITDPIETVSRIDNTTAAVLGAFTFVTATIGINIVANFVSPAFDF
                     SNVAPKYISFRTGGFIAAVASIFITPWNLFNSPEVIHYTLDMLAAFIGPLFGILLVDF
                     YLVRKQQIVVDDLYTESPNGAYWYDKGVNKRAVYALIPAVIIGIVITLSGAKAVADFN
                     WFIGCLLGGVFYSALMSRRPALAGASA"
     gene            2082..2798
                     /locus_tag="PLN02_04190"
     CDS             2082..2798
                     /locus_tag="PLN02_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI51552.1"
                     /translation="MTKTEPTKDAAQERDEIIYQHLHAAICDSRLLPGTRLPEDTLAE
                     TFGASRTIIRKVLQRLAYEGLIEIRPNRGAQIAEPSVPEARDIFATRRILECGALAEM
                     AGKVSAADLAALKAINAAEDEAQLKGDRAAAIRLSGEFHIHLVGLAGNAVLSELVARL
                     IARSSLIIATYGSPISVTCRHSEHEQIIDLIARGAFAEAADWMGEHLTTIERSLRWEQ
                     ETPDLQAVLREFIGGSAAKP"
     gene            2807..3541
                     /locus_tag="PLN02_04195"
     CDS             2807..3541
                     /locus_tag="PLN02_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOI51553.1"
                     /translation="MRIHVINPNTSAAMTASIGRAAERVAAPGTQIIAVNPADGPISI
                     ESHFDEAVAAVGVCEEILRGEAAGADAYVIACFGDPGLAAARELTRAPVLGIAQAAFQ
                     VAAMLATRFSVVTTLQRTVVIAEHLLAAYGYAHQCQRVRAAEIPVLELEDGAACSGIL
                     LAEARRARDEDGAGAIVLGCGGMADYAGAMGEALGIPVIDGVTAAVKLAETLVSLGLH
                     TSKHGDYALPPAKPFAGRFQHLTRSA"
     gene            3543..>3864
                     /locus_tag="PLN02_04200"
     CDS             3543..>3864
                     /locus_tag="PLN02_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HOI51554.1"
                     /translation="MSPTPSYPRDLAGYGRNPPHARWPGQARIAVQFVLNYEEGGENC
                     VLHSDAGSEQFLSEMFNPASYPDRHLSMESIYEYGSRTGVWRILKEFEKRGLPLTVFG
                     VGMAL"
     assembly_gap    3865..3874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3875..>4142
                     /locus_tag="PLN02_04205"
     CDS             <3875..>4142
                     /locus_tag="PLN02_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOI51555.1"
                     /translation="LQRSPAVTAAFVELGHEIACHGWRWIHYQTMDEATEREHMRLAL
                     EVIEQMTGSRPLGWYTGRDSPNTRRLVADDGRLLYVCDYYSDDLP"
BASE COUNT          814 a         1371 c         1294 g          653 t
ORIGIN      
        1 ccagggtacg ccccgggtcg cctttctcga tcagggcggc ggtcgaccgc gagaggccgg
       61 cacgcgccgc cgcatccgcc tggcgaatcc ggcgcgccag acgcagccgg gcgagcttct
      121 cgccgatgga ccgggcttcg gcggcttgct ctggggtcat caagggggtt tcgtcgcgct
      181 tcatgtctgc aatcctggtc attaattaga taatgcctgt gattacggac aaaatactgc
      241 gcagcacgta ttgcgtcaag ccagtcggca aacgcaaaaa ggccgacccg caggtcggcc
      301 ccattgcttg agcacaaagc gtatcaggcg ctcgccccgg ccagcgccgg gcggcggctc
      361 atcagggcgc tgtagaacac gccgccgagc aggcagccga tgaaccagtt gaagtcggcc
      421 accgccttgg caccggacag cgtgatgacg atgccgatga tcaccgccgg gatcagcgcg
      481 tagaccgccc gcttgttcac gcccttgtcg taccagtagg cgccgttcgg gctttccgtg
      541 tacaggtcgt cgacgacgat ctgctgcttg cgcaccaggt agaaatcgac cagcaggatg
      601 ccgaacaagg ggccgatgaa ggcggccagc atgtcgagcg tgtagtggat cacttccggc
      661 gagttgaaca ggttccacgg ggtgatgaag attgacgcga cggcggcgat gaaaccgccg
      721 gtacggaagc tgatgtactt gggcgcgacg ttggagaagt cgaaggccgg cgagacgaag
      781 ttggcgacga tgttgatgcc gatcgtcgcg gtgacgaaag tgaaggcacc gagcacggcg
      841 gcggtggtgt tgtcgatgcg gctgacggtt tcgatcgggt cggtgatcat atggccgaag
      901 accgggatgg tgccggaggt gatgaccacg gtgacgaccg agaaggcgag gaagttgacc
      961 ggcaggcccc agaagttgcc gcgcttcact tcttccatgg tgcggccgta gcgcgagaag
     1021 tcgccgaagt tgagcatcgg gccggagaag taggagacga ccagggcgat ggcggtgatc
     1081 atcatgccca gcgattcgag gccgctgtac ttcacttcgc cgaggttgag cgagatgttt
     1141 tccgggccgg ctttccagac gatccaggcg gccagcgcga acatcacgac gtagaccgcc
     1201 gggccggccc agtcgatgaa gcgcttgatc gcttccatgc cgtgccagaa cacggcggcc
     1261 tgcagcaccc acatggtcat gaagccgacc cagccgaggt aggaaaggcc gaggaactgg
     1321 ctgtgcgtca ggctttccag cccgggaaag aagcgcagca gaacgatgct cagcgccgac
     1381 gaggcgagga aggtctggat gccgtaccag gcaacggcga tcaggccgcg gatgatggcc
     1441 gggatgttgg cgccccagat gccgaaggac atgcggcagg cgaccgggta gggaatgccg
     1501 gccatctggc tcggccgggc gaccagattg cacaggatgt tgaccaggac gatgccgacc
     1561 agcaacgaaa ccagcacctg ccagctgccc agcccgagcg cgaacaggct gccggcaaag
     1621 acgtagccgc cgacgctatg cacatccgac atccagaagg cgaagatgtt gtaccagttc
     1681 cagttcttgt ccttcaacgc ggcgaggtcc tcgttgtaga ggcgctcgct gtattcggcc
     1741 ggctttatat tgttcatggg atttcctttc taggaaaagg caggggtttc aggtactgtt
     1801 gtatacaatt tcaaatccgg ttagcgacaa acaaaaccgg ttttgtccag aaacccagag
     1861 caatcgccgt gccaaattct agcggcagcg accgacagat cgaccgaaga aggcggcaag
     1921 cgtgcaaaaa caacgacttg tattcgatag aaaacaattt tgttgcacac aacgcaatta
     1981 gttgtataca attttgtcga caaatcagtt acacatttgg tgcaattctg aacaagactg
     2041 caccaaaagt aagccaccga gcccgtcagg aaacgcctta catgaccaag accgaaccca
     2101 ccaaggacgc tgcccaggag cgcgacgaga tcatctacca gcacctgcac gccgccatct
     2161 gcgattcgcg cctgctgccc ggcacccgcc tgccggaaga cacgctggcc gagaccttcg
     2221 gcgccagccg gacgatcatc cgcaaggtgt tgcagcgcct ggcctacgaa ggcctgatcg
     2281 agatccgccc gaatcgcggc gcgcagattg ccgagccgtc ggtgccggaa gcgcgcgaca
     2341 tcttcgccac ccggcgcatc ctcgaatgcg gcgcgctggc cgagatggct ggcaaggtca
     2401 gcgccgccga ccttgccgca ctgaaggcaa tcaacgcggc cgaggacgaa gcccagttga
     2461 agggcgaccg cgccgccgcc atccgtcttt ccggcgaatt ccacatccac ctcgtcggcc
     2521 tcgccggcaa tgccgtgctg agcgaactgg tcgcccgcct gatcgcccgc agctcgctga
     2581 tcatcgccac ctatggttcg ccgatctcgg tcacctgccg gcattccgaa cacgagcaga
     2641 tcatcgacct gatcgcccgc ggcgccttcg ccgaagccgc cgactggatg ggcgagcacc
     2701 tgacgaccat cgaacgcagc ctgcgctggg aacaggaaac gcccgacctg caggcggtcc
     2761 tgcgcgaatt catcggcggc tcggccgcca agccttgagg ccgacgatgc gcatccacgt
     2821 catcaacccc aacacctccg ccgcgatgac tgccagcatc ggccgcgctg ccgaacgcgt
     2881 cgccgcgcca ggcacgcaga tcattgcagt taaccccgcc gacgggccaa tttccatcga
     2941 aagccatttc gacgaggcgg tcgccgcggt cggcgtctgc gaggaaatcc tgcgcggcga
     3001 agcggccggt gccgacgcct acgtcatcgc ctgcttcggc gaccccggcc tggccgccgc
     3061 ccgcgagctg acccgggcgc cggtgctcgg catcgcccag gcagccttcc aggtcgccgc
     3121 catgctcgcc acccgctttt cggtggtgac gaccttgcag cgcaccgtgg tcatcgccga
     3181 acacctgctc gccgcctacg gctacgccca ccagtgccag cgcgtgcgcg ccgccgaaat
     3241 cccggtgctt gagctcgaag acggcgccgc ctgcagcggc atcctgctcg ccgaagcacg
     3301 ccgcgcccgc gacgaagacg gcgccggcgc catcgtcctc ggctgcggcg gcatggccga
     3361 ctacgccggg gcgatgggcg aggcgctcgg catcccggtg atcgacggcg tcaccgccgc
     3421 cgtcaagctc gccgaaacac tcgtcagcct cggcctgcac accagcaaac acggcgatta
     3481 cgccttgccg ccggcgaagc ccttcgccgg ccgtttccag catctgaccc ggagtgcctg
     3541 acatgtcacc gacccccagc tacccgcgcg accttgccgg ctacggccgc aacccaccgc
     3601 acgcccgatg gccgggccag gcccgcatcg ccgtccagtt cgtcctcaac tacgaggaag
     3661 gcggcgagaa ctgcgtgctg cacagcgacg ccggctccga gcagttcctt tccgaaatgt
     3721 tcaacccggc cagctacccg gaccgccacc tgtcgatgga gtcgatctac gaatacggct
     3781 cgcgcaccgg cgtctggcgc atcctcaagg aattcgagaa acgcggcctg ccgctgaccg
     3841 tgttcggcgt cggcatggcg ctgcnnnnnn nnnngctgca gcgcagcccg gcagtcaccg
     3901 ccgccttcgt cgaactcggc cacgagatcg cctgccacgg ctggcgctgg attcattacc
     3961 agacgatgga cgaagcgacc gagcgcgagc acatgcgcct ggcgctggaa gtcatcgagc
     4021 agatgaccgg cagccggccg ctcggctggt acaccggccg cgattcgccg aacacccgcc
     4081 gtctggtcgc cgacgacggg cgcctccttt acgtctgcga ttactacagc gacgacctgc
     4141 cg
//
LOCUS       DAOSDX010000140         4130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7622_length_4130_cov_2.733742, whole genome shotgun sequence.
ACCESSION   DAOSDX010000140 DAOSDX010000000
VERSION     DAOSDX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7622_length_4130_cov_2.733742"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PLN02_04210"
     CDS             <1..1269
                     /locus_tag="PLN02_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOI51556.1"
                     /translation="KEYRRYYPSGEMTAHLVGFTGVDDKGLEGVELAFQQSLRGHPGA
                     RTVIRDRRGNIIEDVGATRPPRDGSDIALAIDAKIQYLAYSHLKATVAEHEAKGGGII
                     VLDTRTGEVLALANWPTYNPNNRAKLSGEQLRNRAVTDTFEPGSTLKPFTVALGLEQG
                     KYRYDSLIDCGSGRITVGRSVISDTHANGELTVAQVIQKSSNVGTTKIALSLPRESMW
                     EMFDSLGLGRQPALGFPGEAAGRVRPWKNWRPIEQATMSYGHGISLSLIQLARAYSVF
                     ARDGDLVPVSLLRVDAPVVRGTPVFSAQTAREMRAMLELVVQPGGTAPKAAVPGYRVG
                     GKTGTAHKLEGGVYANKYIASFVGIGPISDPRLIVAVMIDEPSGKQYYGGQIAGPAFA
                     QVMAGALRTLGVPPDAPLQVATTNDGKGRL"
     gene            1269..2744
                     /locus_tag="PLN02_04215"
     CDS             1269..2744
                     /locus_tag="PLN02_04215"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOI51557.1"
                     /translation="MSPRALLDQLESLGVTPTGVADDSRQVRPGDLFLAYPGDLADGR
                     RFIPDALARGAAAVLWQAGGDFAWNAAWSVPQLEVAALRELAGPLAHAVYGHPSEGLS
                     LIAITGTNGKTTISQCIAHAYPRPCAIIGTLGAGFHEQLEETGFTTPEATTLMRYLAD
                     FRARKAAACALEASSIGIEEGRMNGARVDVAVFTNLTRDHLDYHGDMAAYAAAKEKLF
                     LWPRLRTAVINLDDEFGIHLMRETTAMRVIGYSAQPPRRDFPALVRAENRVDTPCGQR
                     FDLVLPNGRTQVDTALVGAYNVSNLLAVAAVLHDAGVMPAEVGRRLSELQPPPGRMER
                     LGGSGEPLVVVDYAHTPDALHNALAALRPVADARGGRLVVVFGCGGDRDPGKRPQMGA
                     VAEAGADRVIVTSDNPRREAPAAIIADIVGGMERPEIEADRATAIRRAVSVADVADVV
                     LLAGKGHENYQEIAGQRLPFSDLAQARAALAGRSAPQEEAC"
     gene            2738..4111
                     /gene="murF"
                     /locus_tag="PLN02_04220"
     CDS             2738..4111
                     /gene="murF"
                     /locus_tag="PLN02_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOI51558.1"
                     /translation="MLNWSLSRVAAAVDGRLCGADVAVRGVSTDTRAVAAGQLFVALR
                     GERFDAHDFLDAAVAAGAAALLVADESRLPAGMPAIVVADTRLALGRLAQAWRAEFTL
                     PVIAVTGSNGKTTTKEMIAAILAAAFGDAVLATRGNFNNDIGLPLTLLGLNAGHRAAV
                     IEMGMNRPGEIGYLVGIGAPSVALVTNAQRAHLEGMGDLDEVAREKGSIFAGLPADGI
                     AVINADDGYAALWREMAGGRRVRSFAIDHPADVHGNVYQHGLEISLALTADEGAIQFT
                     LAIPGRHNARNAVAAAAACLAAGVPLTAVAEGLSSFAGVKGRLQRRTGRKGAEILDDT
                     YNANPDSVRAGIDVLAATIGRKLLVLGDMGEIGEASGQYHDEIGGYAKSQGIDRLYAL
                     GEASQQAVRNFGEGARHFCHIDKLLAAVDKELGPDTTVLIKGSRFMKMERVADALAAT
                     AAIGENH"
BASE COUNT          655 a         1443 c         1399 g          633 t
ORIGIN      
        1 aaggaatacc gccgctacta tccgtcgggc gagatgaccg cgcatctggt cggtttcacc
       61 ggcgtcgacg acaagggcct ggaaggcgtc gagctggcct tccagcagtc gctgcgcggt
      121 catcccgggg cgcgtacggt gatccgcgac cgccgcggca acatcatcga ggatgtcggc
      181 gccacccggc cgccgcgcga tggcagcgac atcgcgctgg ccattgatgc caagatccag
      241 tacctcgcct acagccacct caaggcgacg gtggccgagc atgaagccaa gggcggcggc
      301 atcatcgttc tcgatacgcg taccggcgaa gtgctggcgc tcgccaactg gccgacctat
      361 aacccgaaca accgcgccaa gctgtccggc gagcagttgc gtaaccgtgc ggtcaccgac
      421 accttcgagc ccggctcgac gctgaagcca ttcaccgtcg cgcttggtct ggagcagggc
      481 aagtaccgct acgattcgct gatcgactgc gggtcgggac ggatcaccgt cggccgctcg
      541 gtgatcagcg atacccacgc caacggcgaa ctgaccgtcg cccaggtaat ccagaagtcg
      601 tcgaacgtcg gcaccaccaa gatcgccctc agcctgccgc gtgagtcgat gtgggaaatg
      661 ttcgacagcc tcggcctcgg ccgccagccg gcgctcggtt tcccgggtga agccgccggt
      721 cgggtgcggc cgtggaagaa ctggcggccg atcgagcagg cgacgatgtc ctacgggcac
      781 ggcatttcgc tcagcctgat ccagctcgca cgagcctact cggtattcgc ccgcgatggc
      841 gatctggtgc cggtgtcgct gctgcgcgtc gatgcgccgg tggtgcgcgg tacgccggtg
      901 ttctcggcgc agacggcgcg cgaaatgcgc gccatgctcg aactggtcgt gcagcccggc
      961 ggtaccgcgc cgaaggcggc ggtgccgggt taccgggtcg gcggcaagac cggtaccgcg
     1021 cacaagctgg aaggtggcgt gtacgccaac aagtacatcg cctccttcgt cggcatcggg
     1081 ccgatctccg atccccggct gatcgtcgcc gtgatgatcg acgagccgag cggcaaacag
     1141 tattacggcg gccagattgc cggcccggcg tttgcgcagg tgatggccgg tgccctgcgc
     1201 accctcggcg tgccgccgga tgcgccactg caggtggcta ccacgaatga cggaaaggga
     1261 cgcctgtgat gagcccgcgc gccctgctcg atcaactgga aagcctcggc gtgacgccga
     1321 ccggcgttgc cgacgacagc cgccaggtgc gcccgggcga tcttttcctg gcctatcctg
     1381 gcgatctcgc cgacggtcgg cgtttcatcc cggatgccct ggcgcgcggt gccgccgctg
     1441 tcctctggca ggcaggtggc gacttcgcgt ggaacgcggc gtggtcggtg ccgcaactgg
     1501 aagtcgccgc gctgcgcgaa ctggccggtc cgctggcgca tgccgtgtac ggtcatccga
     1561 gcgaggggct gtcgctgatc gccatcaccg gcaccaacgg caagacgacg atcagccagt
     1621 gcatcgccca cgcctatccg cgcccctgcg ccatcatcgg cacgctcggc gccggctttc
     1681 acgagcaact ggaggaaacc ggcttcacga cgcccgaggc gacgacgctg atgcgttacc
     1741 tggccgactt ccgtgcccgc aaggcggccg cctgtgcgct cgaagccagc tcgatcggca
     1801 tcgaggaagg gcggatgaat ggcgcccgcg tcgatgtcgc ggtgttcacc aacctgaccc
     1861 gcgatcacct cgactaccac ggcgacatgg ccgcctacgc cgccgccaag gaaaagctct
     1921 tcctctggcc gcgcctgcgt accgcggtga tcaacctcga cgacgaattc ggcatccacc
     1981 tgatgcgcga gacgacggcg atgcgcgtca tcggctattc agcgcagccg ccgcggcgtg
     2041 atttcccggc gctggtgcgc gccgagaacc gcgtcgacac gccttgcggt cagcgcttcg
     2101 acctcgtcct gccgaatggg cggacccagg tcgacacggc gctggtcggc gcctacaacg
     2161 tctccaacct gctggccgtc gccgccgtgt tgcacgacgc tggcgtgatg ccggccgagg
     2221 tcggccgccg tctctccgag ttgcagccgc caccagggcg gatggagcgt ctgggtggca
     2281 gcggcgagcc gctggtcgtt gtcgattacg cccacacccc ggatgccctg cacaacgccc
     2341 tggccgcgtt gcgcccggtg gccgatgcgc gcggtggccg cctggtcgtc gtcttcggtt
     2401 gcggcggcga ccgcgatccg ggcaagcgtc cgcagatggg cgcggtggcc gaagctggcg
     2461 ccgatcgcgt catcgtcacc agcgacaacc cgcgccgcga ggcgccggcg gcgatcatcg
     2521 ccgacatcgt cggcggcatg gaaagaccgg aaatcgaagc cgaccgggcg acggcgatcc
     2581 gtcgcgccgt gagcgtcgcc gatgtcgccg acgtcgtgct gctggccggc aagggacacg
     2641 aaaactatca ggaaatcgcc ggccaacgcc tgccgttttc cgatctggcc caggcccgtg
     2701 cggcgctggc cggacgctct gccccgcagg aggaagcatg ctgaactggt ccttgtcccg
     2761 tgtcgccgca gccgtcgacg gtcgcctgtg cggcgccgac gtcgccgtgc gcggtgtgtc
     2821 caccgatacc cgggcggttg ccgccggcca gcttttcgtt gccttgcgcg gcgagcgctt
     2881 cgatgcccac gatttcctcg acgccgccgt tgccgccggc gccgcagcgc tgctggtggc
     2941 tgacgagagc cgcctgccgg ccgggatgcc ggccatcgtc gtcgccgaca cgcggctcgc
     3001 gctgggtcgt ctggcgcagg cctggcgggc cgaattcacg ctgccggtga tcgccgtcac
     3061 cggttccaac ggcaagacga cgaccaagga aatgatcgcc gccatcctcg ccgccgcctt
     3121 cggcgacgcg gtgctggcga cgcgcggcaa cttcaacaac gacatcggcc tgccgctgac
     3181 cttgctcggc ctcaatgccg ggcaccgcgc ggcggtgatc gagatgggca tgaaccggcc
     3241 cggcgaaatc ggctacctgg tcggcatcgg cgcgcccagc gtggcgctgg tcaccaacgc
     3301 ccagcgcgcc catctcgagg gcatgggcga cctcgacgaa gtggcgcgtg agaaaggcag
     3361 catcttcgcc ggtctgcccg ccgacggcat cgccgtgatc aacgccgacg acggctacgc
     3421 cgcgctgtgg cgtgagatgg ccggcggccg ccgcgtgcgc agtttcgcca tcgaccatcc
     3481 ggccgacgtg cacggcaacg tgtaccagca cggcctggaa atttccctcg ccctgaccgc
     3541 ggacgaaggc gcgatccagt tcacgctggc catccccggg cgccacaacg cgcgcaatgc
     3601 ggtggccgcc gccgctgctt gcctggctgc cggcgtgccg ttgaccgcgg tggccgaggg
     3661 cctgtccagc tttgccggcg tcaaggggcg gctgcaacgg cgtactggcc gcaagggcgc
     3721 cgaaatcctc gacgacacct acaacgccaa cccggattcg gtgcgcgccg ggatcgacgt
     3781 gctggccgcc accatcggcc gcaagctgct ggtgctcggc gacatgggcg aaatcggcga
     3841 ggccagcggc caatatcacg acgagatcgg cggctacgcc aagagccagg gcatcgatcg
     3901 cctgtacgcg ctcggcgagg cgagccagca ggcggtgcgc aacttcggcg agggggcacg
     3961 tcacttctgc catatcgaca agctgctcgc cgccgtcgac aaggaactgg gtccggacac
     4021 cacggtgctg atcaagggct cgcgcttcat gaaaatggaa agggtggccg atgcactggc
     4081 cgccaccgcc gctatcgggg agaaccactg atgcttctgg aacttgccca
//
LOCUS       DAOSDX010000141         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7628_length_4126_cov_3.993368, whole genome shotgun sequence.
ACCESSION   DAOSDX010000141 DAOSDX010000000
VERSION     DAOSDX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7628_length_4126_cov_3.993368"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLN02_04225"
     CDS             complement(<1..322)
                     /locus_tag="PLN02_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51559.1"
                     /translation="MKFLGDTILGLTYSPEEITDEPNGILVLRANNIQDRRLVANDPV
                     YVRADAPEKLKLRVGDILICSRNGSRHLIGKCGRVTAEFVSCYFGGRFQRPSATFGII
                     PALSM"
     gene            complement(403..1431)
                     /locus_tag="PLN02_04230"
     CDS             complement(403..1431)
                     /locus_tag="PLN02_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOI51560.1"
                     /translation="MTSGIDAKPGGEFVLYQTEDGQSRIQCQLVDGTIWLSQMQMGEL
                     FQRSKQTISEHLQNIFEEGELPEDSVVRNFRTTAADGKAYDVTHYALEAIIAVGMRVK
                     SVRGTQFRRWANTQLKEFIVKGFVMDDERLKNPGGWDYFDELLARIRDIRASEKRFYQ
                     KVRDLFALSDDYRADDSAAQQFFAEVQNKLLYAVTRKTAAQIVVDRSNPDAPNMALTA
                     WSGNRVRKQDVIVAKNYLTADEVDTLNRLVVIFLEQAELRVKERKQLTLDYWRQNVDR
                     LLEFNERPVLQGAGRISAEQMKGIAHERYEVFDARRRSAEALAADAEDLRMLESLEKQ
                     AKGKGKGN"
     gene            complement(1428..3719)
                     /locus_tag="PLN02_04235"
     CDS             complement(1428..3719)
                     /locus_tag="PLN02_04235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOI51561.1"
                     /translation="MKNYEALKQQGDVIWNIANLLRGPYRPPQYRRVMIPLTVLRRLD
                     CVLESSKEQVIDYHRKLKADGKYDAETIETMINHRFKLSFHNTSEFTFQNLLGDADKL
                     AANLNNYIAGFSARARKIIEKFKFDEEIEKLDEANRLFEVVKQIAAIDLHPDRITNIE
                     MGYLFEDLVRRFNEQANEEAGDHFTPREVIKLMVSILFTHDDLVYREGKVIKIYDPTC
                     GTGGMLSESDKLILHPETGLNPGANLQLFGQEYNPESYAICGSDLMIKGEEVKNIVFG
                     NTLGTGKSKEGFVDGDGHPDERFHYMLANPPFGVEWKPEKDNVTREHNEFGFKGRFGP
                     GLPRINDGALLFLLHMISKMRPAPEKGGDGSRIAVVFNGSPLFTGDAGSGESNIRRWI
                     IENDMLEAVIGLPDQLFYNTGISTYVWIVTNRKRPQRAGKVQLINATEFARKMKKSLG
                     DKRKRLGDGTDGTNSQPDGSPDHIAQITRLYGEFQHDVRMTLAEIQTNIDPKRDQTKS
                     LFVSKIFDNREFGYLKITVERPLRLNFAVTDERITRFQQGSFFESLAISLKRKNKARI
                     EAEILEGKAAQAAILAVLHAMKAAFASGEPIKDRKVFEAQLSEAFQAADLTLDLPLKA
                     ALLAPGALGEKDPTAEICRDKKGNPEPDADLRDTENVPLPEDIDLPLPLDYESKKNKG
                     KVDVEPLLKRVKAHCQAYLQAEVLPYRPDAWIEHSKIKVGYEIPFNRHFYEYEPPRPL
                     EAIEADIEQLEGEIMAMLKAVTA"
BASE COUNT          797 a         1107 c         1200 g         1022 t
ORIGIN      
        1 tcatggacaa agcagggatg attccaaatg ttgcactcgg tctttgaaac cgcccaccaa
       61 agtagcagct gacaaactcc gccgttactc gaccgcattt tccgattaga tgcctgctcc
      121 catttcgtga acagatcagg atatcgccaa ctcgcagctt cagcttttca ggcgcatccg
      181 cgcggacata taccggatca tttgctacaa gtcggcgatc ttgaatattg ttggcacgga
      241 gaaccaaaat gccattgggt tcatcagtaa tttcctctgg ggaataggta aggccaagaa
      301 ttgtgtcgcc aagaaacttc aagcgttttt cagaccatcc gtgaggaagt tccccgagcc
      361 aatcgacgga actggattcg tattttgggt atctcaaata cctcaattgc ccttcccctt
      421 ccccttcgcc tgcttctcca gcgactccag catccgcaaa tcctcggcat ccgccgccag
      481 tgcctcggca ctacgccgcc gcgcgtcgaa gacctcgtag cgttcgtggg cgatgccctt
      541 catctgttcg gcactgatcc ggccagcccc ttgcagcacc gggcgttcat tgaattccag
      601 caggcgatcg acgttctggc gccaataatc cagcgtcagt tgcttgcgtt ccttgacgcg
      661 cagttcggcc tgttcgagaa agatgacgac caggcgattg agcgtatcca cttcgtcagc
      721 cgtcaggtag ttcttggcga cgatgacatc ctgcttgcgc acgcggttgc cgctccaggc
      781 ggtcagcgcc atgttcgggg cgtccggatt gctgcggtcg acgacgatct gggcggcggt
      841 tttgcgggtg accgcgtaga gcagcttgtt ctgcacttcg gcaaagaact gctgagcggc
      901 gctgtcgtcg gcccggtagt cgtcgctcag ggcgaagaga tcgcgcacct tctggtagaa
      961 acgcttttcg gaagcgcgta tgtcgcggat gcgcgccagc agttcgtcaa agtagtccca
     1021 gccaccgggg ttcttgaggc gttcgtcgtc catgacgaaa cctttgacga tgaattcctt
     1081 gagctgggta ttggcccagc gccggaactg ggtgccgcgc acggatttga cccgcatgcc
     1141 gacggcgatg atggcttcca gcgcgtagtg cgtgacatcg taggctttgc catcggcggc
     1201 agttgtccgg aaattccgga caactgaatc ctctggcaat tcgccttctt caaagatgtt
     1261 ttgcaagtgc tcgctgatgg tttgcttgga gcgctgaaac agctcgccca tctgcatttg
     1321 cgatagccag atggtgccgt cgaccagctg gcactggatg cggctctggc cgtcctcggt
     1381 ctgatagaga acgaattcgc cgccgggctt ggcgtcgatg ccgctagtca tgcggtcacc
     1441 gccttgagca tggccatgat ttctccttcg agttgctcga tatcggcctc gatggcttcc
     1501 agcgggcgcg gtggctcgta ttcgtagaaa tggcggttga aggggatttc gtagccgacc
     1561 ttgatcttgc tgtgctcgat ccaggcgtcg ggacggtaag gcagcacttc ggcttggaga
     1621 taggcctggc agtgtgcctt tacccgcttc agcaagggtt cgacatcgac cttgcctttg
     1681 ttcttcttgc tttcgtaatc gagcggcagc ggcaggtcga tgtcttccgg caagggcacg
     1741 ttttcggtgt cgcgcaagtc ggcatcgggt tcgggattgc cctttttatc gcggcagatt
     1801 tcggcagtag ggtctttttc gcccagtgct cccggcgcga gcaaggcggc tttcaggggc
     1861 aggtcgaggg tcagatcggc agcctggaag gcttcactca gttgcgcctc gaataccttg
     1921 cggtccttga tcggttcgcc actggcaaag gcggccttca tggcatgcag cacggccagg
     1981 atggcggcct gggcggcttt gccttcaaga atttctgctt cgattctggc cttgttcttg
     2041 cgcttgagcg aaatggctag cgattcgaaa aaactgcctt gctgaaagcg cgtgatgcgt
     2101 tcgtcggtca ctgcaaaatt gaggcgcagc gggcgttcga cggtgatttt cagatagccg
     2161 aactcgcggt tgtcgaagat tttgctgacg aacagtgact ttgtctggtc acgcttgggg
     2221 tcgatgttgg tctgaatctc ggccagcgtc atccggacat cgtgctggaa ttcgccatag
     2281 agccgcgtga tctgggcaat gtggtcgggc gagccatcgg gttggctgtt ggtgccgtcg
     2341 gtgccgtcac ccagccgttt gcgcttgtcg cccaggcttt tcttcatctt gcgggcgaat
     2401 tcggtggcgt tgatgagttg aaccttgcct gcccgttgcg ggcgcttgcg gttggtgacg
     2461 atccacacgt aggtggagat accggtgttg tagaagagct ggtcgggcag gccgatgacc
     2521 gcttcgagca tgtcgttttc gatgatccag cgacggatgt tgctctcgcc gctgccggcg
     2581 tcgccggtaa acaagggcga accgttgaag acaacggcga tgcgcgagcc atccccgcct
     2641 ttttcaggcg ccggccgcat cttggaaatc atgtgcagta gaaagagcag cgcgccatcg
     2701 ttgatgcgcg gcagaccggg accgaatcga cccttgaagc cgaattcgtt gtgctcgcgc
     2761 gtcacgttgt ccttttccgg cttccattcc acaccaaagg gcggattggc cagcatgtaa
     2821 tggaaacgct cgtccggatg gccgtcgcca tcgacgaatc cttccttgct cttgccggtg
     2881 cccagggtat tgccgaagac gatgttcttg acctcttcgc ccttgatcat caggtcggag
     2941 ccgcaaatgg cgtaggactc ggggttgtat tcctggccga agagttgcag gttggcgccc
     3001 ggattgaggc cggtttcggg gtggagaatc agtttgtcgg attcggacag catgccgccg
     3061 gtgccgcagg ttgggtcgta aatcttgatg accttgcctt cccggtacac cagatcgtca
     3121 tgggtgaaca ggatgctgac catcagcttg atgacttcgc gcggcgtgaa atggtcgcca
     3181 gcctcttcat tggcttgttc gttgaaacgc cggaccagat cttcgaacag gtagcccatt
     3241 tcgatgttcg tgatgcgatc cgggtgcagg tcgatggctg caatctgctt gaccacctca
     3301 aacaagcgat tggcttcgtc cagcttctcg atttcttcat cgaatttgaa cttctcgatg
     3361 attttccgcg cccgcgccga aaaaccggcg atgtagttgt tcaggttggc cgccaattta
     3421 tcggcatcac ccagcaggtt ctggaaggta aattcactgg tgttgtggaa actgagcttg
     3481 aagcgatgat tgatcatcgt ctcgatggtt tcggcgtcgt acttgccatc ggccttaagc
     3541 ttgcggtgat agtcgatcac ctgctccttg ctgctctcca gcacgcaatc gagacggcga
     3601 agcacggtca gcggaatcat tacccggcga tactggggcg gacggtaggg accacgcaaa
     3661 agattggcga tgttccagat cacatcgcct tgttgtttga gcgcttcgta gtttttcatc
     3721 gaatgttttt ttcccgaaac atcaggtcgc acaagcaggc gtgcgtaaaa atgaaaatgg
     3781 tattccaaaa aacagaaaga ggcttggtta gcctctttct tgggtttgtc gcgctttttg
     3841 cgtgatcagg aaaaacctgt tggatgacat agaaagtgaa aatagccatc gtggcaacaa
     3901 acgacttttc ctttaaattt gtcatcaaag cactcccgtc gtgccaagga aactgaaaaa
     3961 aaccggattt catgtcacca aaagcgaaaa atcccgagac atcatctcat gcggcattgc
     4021 ttaccaatgc ccaggctttc ggattggctt tttatttgtt gggacatgat ccggcagctg
     4081 ggaagcttcg agctccgaat ctaatataga ttggcgcgtc cagtgg
//
LOCUS       DAOSDX010000142         4104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7682_length_4104_cov_3.120030, whole genome shotgun sequence.
ACCESSION   DAOSDX010000142 DAOSDX010000000
VERSION     DAOSDX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7682_length_4104_cov_3.120030"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLN02_04240"
     CDS             <1..1335
                     /locus_tag="PLN02_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOI51562.1"
                     /translation="KEPFYLVCGCGETGSLICHNFDRIGQAFVVIEKDELRVEELDLL
                     DFKTDTPALAADASLPDNLLLAGLQHPKCRGVLAVTNDELANLAIAIAVRLLAPKLPV
                     IARARSQSVAANMASFGTDHIINPFERFADHLCQAITAPERFRLVELLTSLPGSPLPQ
                     PHRPPRGHWIVCGYGRFGQAVAARLREAGLTLTIVEPHASPADEHVVAGDGTEAATLR
                     AAGIEQADGIIAGSDNDVNNLSIAVTASELRPALFVVTRQNQAANNALFQAYGPEFAM
                     VPSHIVAHESIAILTTPLLGRFLAALDQRDEAWCRKLASRLQEIGGDRTPTVWSIRLN
                     ASEAPAARQALMHGRPFRLGAILRDANDRSSALPLSVLLIDRSDRIHLLPDDDFELSA
                     GDHLLLASHLGARRRLELALQNLNELAYVLDGETRPSGALWRILTPRKKPLP"
     gene            1332..2519
                     /locus_tag="PLN02_04245"
     CDS             1332..2519
                     /locus_tag="PLN02_04245"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI51563.1"
                     /translation="MNDPLTQARDHFLRGLAAFEAGDDLAARAAFVAALALAPGRPSV
                     MVNLAATLLRLRDFAAARELLLEITAIEPDNREARVQLGLCEEALGHWAAAAAALESG
                     LPATASPELWLVCSHCHGRAGELPAALAAIDRVLALVPEHAEAWSTRGGLLRELGRLP
                     EAADAYRRALQHGGDPELNRYYLAAVGDDAPPATTPSHYVQALFDDYAPDFQQHLVDG
                     LRYQGHRRLIEPLLQAGRRYPAAVDLGCGTGLCGELLRPLVDAIDGIDLSAAMLEQAR
                     GRGIYRHLERHELNAYLAARTTPADLFVAADVFGYIGDLAPAFAAVRRLLRAGGCFAF
                     TVEQLLDGGDYTLQPCLRYRHSEDYVRRLAAQHGFTVRELAADTLRHDQQTPIPALYV
                     YLA"
     gene            complement(2507..>4104)
                     /locus_tag="PLN02_04250"
     CDS             complement(2507..>4104)
                     /locus_tag="PLN02_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOI51564.1"
                     /translation="AADPHLRRAAQVYAALAAGLFCGMLAQASWTFVAAEMAVPRAIA
                     YGIPGWWFMAALPAGFLLLGVRLAARLPAAANWRWLAAGALPALGWWFAGHYDGSVLP
                     LVPFAGALLLLLLCGAPVFAVLGGLALALFWSEGQPLAAVPLSHYQITVNPSLPALPL
                     FTLAGLVFARTGAAARLGSLFTALFGGGARGTAIAAAVLCSGFTALTGGSGVTILALG
                     GLLLPLLVGAGFPERRGIGLVTSASALGVLLAPSVPLIMYAIIARVPINAMFLAGLLP
                     ALVMVVFLLLVGGFLKRDGLAPTSAVGRVDFAAARRAAWAAKWEILAPLVAVGSLVGG
                     WATPTESAALTAAYALLTQSLLLREIDARGVGRALVECAQVIGGIMLILGMALGLTNY
                     LVDAGIPDRAIDGVQALLPDKWSFLIALNLFLLLAGALMEIYAAIVVLVPLLLPVALA
                     YGIDPVHFGIIFLANLEMGFLCPPAGMNLYFSAAMFGKPVREIAVSVLPAVLAIFLGA
                     LTLSLLPFLSTALPDWLLGVQAR"
BASE COUNT          678 a         1597 c         1350 g          479 t
ORIGIN      
        1 aaggagccgt tctacctggt ctgcggctgc ggcgagaccg gcagcctgat ctgccacaac
       61 ttcgaccgca tcggccaggc cttcgtcgtc atcgaaaagg acgaactgcg cgtcgaggaa
      121 ctcgacctcc tcgacttcaa gaccgacacc ccggccctgg ccgccgacgc cagcctgccc
      181 gacaacctgc tgctggccgg cctgcagcac ccgaaatgcc gcggcgtact ggcggtgacc
      241 aacgacgaac tggccaacct ggcgatcgcc atcgccgtcc gcctgctcgc ccccaagctg
      301 ccggtgatcg cccgcgcccg cagccagtcg gttgccgcca acatggcctc gttcggcacc
      361 gaccacatta tcaacccctt cgagcgcttc gccgaccacc tctgtcaggc gatcaccgcc
      421 cccgagcgct tccgcctcgt cgaactgctg accagtctgc ccggctcgcc gctgccgcaa
      481 ccgcaccggc cgccgcgcgg ccactggatc gtctgcggct acggccgctt cggccaggca
      541 gtcgccgccc gcctgcggga ggccgggctc accctgacca tcgtcgaacc ccacgcttca
      601 cctgccgacg agcacgtcgt cgccggcgac ggcaccgaag ccgccacgct gcgcgccgcc
      661 ggcatcgagc aggcggacgg catcatcgcc ggcagcgaca acgacgtgaa caacctgtcg
      721 atcgccgtca ccgccagcga actgcggccg gcgctgttcg tcgtcacgcg ccagaaccag
      781 gccgccaaca acgcgctgtt ccaggcctac ggaccggaat tcgcgatggt gccgagccac
      841 atcgtcgccc acgagagcat cgccatcctc accacgccgc tgctcggccg cttcctcgcc
      901 gccctcgacc agcgcgacga ggcctggtgc cgcaaactgg ccagccgcct gcaggaaatc
      961 ggcggcgacc gcacgccgac cgtatggagc atccgcctca acgccagcga agcgccggcc
     1021 gcccgccagg cgctgatgca cggccgcccc ttccgtctcg gcgccatcct gcgcgacgcc
     1081 aacgatcgca gcagcgccct gccgctgagc gtcctgctga tcgaccgcag cgaccgcatc
     1141 cacctgctgc cggacgacga cttcgagctc agcgccggcg accacctgct cctcgccagc
     1201 cacctcggcg cccgccgccg tcttgaactg gcgctgcaga acctgaacga actggcctac
     1261 gtgctggacg gcgagacccg cccgtccggc gccctatggc gcatcctcac cccacgcaag
     1321 aaaccgctgc catgaacgat cccctcaccc aggcccgcga ccacttcctg cgcggcctcg
     1381 ccgctttcga ggcgggcgac gatctggccg cccgcgccgc cttcgtcgcc gcgctggcgc
     1441 tggcgccggg gcggccgtcg gtgatggtca acctggcggc gaccctgctc cggctgcgcg
     1501 atttcgccgc cgcccgcgaa ctgctgctgg aaatcaccgc cattgagccg gacaaccggg
     1561 aagcgcgcgt ccagctcggc ctctgcgagg aagccctcgg ccactgggcg gcggccgccg
     1621 cggcgctgga aagcggcctg ccggcgacgg ccagcccgga gctgtggctg gtctgcagcc
     1681 actgccatgg ccgggccggc gagctaccgg cggcgctcgc cgcgatcgac cgcgtcctcg
     1741 ccctggttcc tgagcacgcc gaggcgtgga gcacgcgcgg cggcctgctg cgcgaactcg
     1801 ggcggctgcc ggaagccgcc gacgcctatc ggcgcgcgct gcagcacggc ggcgaccccg
     1861 aactcaaccg ctattacctg gccgccgtcg gcgacgacgc cccgccggcg acgacgccga
     1921 gccactacgt gcaggcgctg ttcgacgact acgcgccgga cttccagcaa cacctggtcg
     1981 acggcctgcg ctaccagggc caccgccggc tgatcgagcc gctgctgcag gccggccggc
     2041 gctacccggc ggcggtcgac ctgggctgcg gcaccggtct ctgcggcgaa ctgctgcggc
     2101 cgctggtcga cgccatcgac ggcatcgacc tttccgccgc catgctcgaa caggcgcgtg
     2161 gacgcggcat ctaccgccac ctcgaacgcc acgagctgaa cgcctatctc gccgcccgca
     2221 cgacgccggc cgaccttttc gtcgccgccg acgtcttcgg ctacatcggc gacctcgccc
     2281 cggcctttgc cgccgtccgc cgcctgctcc gggccggcgg ctgcttcgcc ttcaccgtcg
     2341 agcaactgct tgatggcggc gactacacgc tgcagccctg cctgcgctac cggcacagcg
     2401 aggactacgt gcgccggctg gccgcgcagc acggcttcac ggtccgcgag ctggccgccg
     2461 acaccctgcg ccacgaccag cagacgccga tcccggcgct ctacgtctac ctcgcctgaa
     2521 cgccgagcag ccagtcgggc agcgcggtgc tcaggaaggg cagcagcgac agcgtcagcg
     2581 caccaaggaa gatcgccagc accgccggca gcaccgagac ggcgatctcg cgcaccggct
     2641 tgccgaacat cgccgccgag aaatacaggt tcatgccggc cggcgggcag aggaagccca
     2701 tctccaggtt ggccaggaag atgatcccga aatgcaccgg gtcgatgccg taggcgagcg
     2761 ctaccggcaa caggagtggc accaggacga cgatcgccgc gtagatctcc atcagcgccc
     2821 cggccagcag caggaacagg ttcaaggcga tcaggaagct ccatttgtcg ggcagcaagg
     2881 cctgcacgcc gtcgatcgcc cggtcgggga tgccggcatc gaccaggtag ttggtcaggc
     2941 cgagcgccat gccgaggatc agcatgatgc cgccgatcac ctgcgcgcat tcgaccagcg
     3001 cccggccgac gccgcgcgcg tcgatctcgc gcagcagcaa tgactgcgtg agcagcgcat
     3061 aggcggcggt cagggcggcg ctctcggtcg gcgtcgccca gccgccgacc agcgaaccga
     3121 cggcgacgag cggcgccagg atttcccatt tcgccgccca ggcggcacgg cgggcggcgg
     3181 cgaaatcgac ccggccgacc gcactcgtcg gcgccaggcc gtcgcgcttc aggaagccgc
     3241 cgaccagcag caggaagacc accatcacca gcgccggcaa tagcccggcg aggaacatcg
     3301 cgttgatcgg cacgcgggcg atgatcgcgt acatgatcag cggcaccgac ggcgccagca
     3361 gcacgcccag cgcgctggcg ctggtcacca ggccgatgcc gcgccgctcc gggaagccgg
     3421 cgccgaccag cagcggcagc agcaggccgc cgagcgccag gatggtgacg ccgctgccgc
     3481 cggtcagcgc ggtgaaaccg gaacagagga cggcggcggc gatcgccgtg ccgcgcgcac
     3541 cgccgccgaa cagcgcggtg aacaagctcc ccaggcgggc cgccgcgccg gtgcgggcga
     3601 acaccagacc ggccagggtg aatagcggca aggccggcag cgagggattg acggtgatct
     3661 ggtaatggct gagcggcaca gccgccagcg gctggccctc gctccagaac agcgccagcg
     3721 ccaggccgcc gagcacggca aagaccggcg cgccgcacag cagcaggagc agcagggcgc
     3781 cggcaaacgg caccagcggc aatacgctgc cgtcgtagtg cccggcaaac caccagccga
     3841 gcgccggcag cgccccggcg gcgagccagc gccagttcgc cgcggcaggt agccgagcgg
     3901 cgagacggac accgagcagc aggaagccgg ccggcagcgc cgccatgaac caccagccgg
     3961 gaatgccgta ggcgatggcg cgcggcaccg ccatctcggc agcgacgaag gtccagctcg
     4021 cctgcgccag catgccgcag aaaaggcccg cggcgagcgc cgcgtagact tgcgcggcgc
     4081 ggcgcaggtg gggatcggcg gcgg
//
LOCUS       DAOSDX010000143         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7699_length_4096_cov_3.992824, whole genome shotgun sequence.
ACCESSION   DAOSDX010000143 DAOSDX010000000
VERSION     DAOSDX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7699_length_4096_cov_3.992824"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1837
                     /locus_tag="PLN02_04255"
     CDS             41..1837
                     /locus_tag="PLN02_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOI51565.1"
                     /translation="MGAYETQGFRLDNSGRRVVVDPVTRIEGHMRCEVNLDSNNVIRN
                     AVSTGTMWRGLEVILKGRDPRDAWAFTERICGVCTGTHALTSVRAVEDALAIKIPENA
                     NTIRNLMQLNLYVHDHLVHFYHLHALDWVDVVSALKADPKATSTLAQSISSWPLSSPG
                     YFRDIQNRLKKFVESGQLGPFMNGYWGNPAYKLPPEANLMAVAHYLEALDFQKEIVKV
                     HTIFGGKNPHPNWLVGGVPCAINLEGTGAVGAVNMERLNLVKSIIDRCTEFVEQVYIP
                     DLLAIGSFYKGWLHGGGLSSKNLLSYGDIPQIANDYSAGNLLLPRGAIINGKLDEIHP
                     IDLKDPEQIQEFVAHSWYKYPDETVGLHPFDGVTEPNFVLGPNTKGTKTNIKELDEGA
                     KYSWIKAPRWRGHAMEVGCLSRMVLGYLQPKQYPEIHALVDGALKHLDVPVTALFSTL
                     GRTAARGLETAYCVKLQSQQFDKLMANLKAGDLNTANIEKWEPSTWPKEAQGAGFTEA
                     PRGALGHWIKIKDTKIDNYQCVVPTTWNGGPRDHKGQIGAFEASLMDTPVAKADEPLE
                     ILRTLHSFDPCLACSTHVMSPDGQEMTSVKVR"
     gene            1860..2564
                     /gene="cybH"
                     /locus_tag="PLN02_04260"
     CDS             1860..2564
                     /gene="cybH"
                     /locus_tag="PLN02_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HOI51566.1"
                     /translation="MLSQQDMLEAERHGRQVRSIYVYEAPVRLWHWVNALAMVVLAVT
                     GYFIGKPLPTVPGEAAENFVMGYIRFAHFAAAYIFAVGLLGRIYWAAVGNHHARQIFK
                     LPITNPQWWSEVFFELRWYLFLEKTPKKYVGHNPMAQLMMFFFFTVLAVGMVFTGFAL
                     YSEGAGLGSWQDALFGWVIPALGGSLQVHNLHRLGMWIMMTFAVVHIYAAIREDIMSR
                     QSLISTMVSGWRMFKD"
     gene            2642..3253
                     /locus_tag="PLN02_04265"
     CDS             2642..3253
                     /locus_tag="PLN02_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HOI51567.1"
                     /translation="MEKRILVLGIGNLLWADEGFGVRCVEALNASWQFPPQVTLMDGG
                     TQGLYLLPYVQEADCLLVFDAVDYGDTPGDLREVVGDQVPRFMGAKKMSLHQTGFQEV
                     LMAAELTGKLPAELVLIGVQPEELEDFGGSLRDVVKAQMAPALNLALDWLERWGATGR
                     LRDSEAEAITDAALAIDLYEGERPPADQAYRLGDARFLNLEAA"
     gene            3253..3555
                     /gene="hypC"
                     /locus_tag="PLN02_04270"
     CDS             3253..3555
                     /gene="hypC"
                     /locus_tag="PLN02_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOI51568.1"
                     /translation="MCLGIPAQVIECGEHYARCQGPDGEQRIDIRLVGAQAPGTWLLC
                     FLDAAREVIDAERATAITAALAALDAVNAGETDLSAFFADLDREPQLPEFLRTTHP"
     gene            3552..4010
                     /locus_tag="PLN02_04275"
     CDS             3552..4010
                     /locus_tag="PLN02_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51569.1"
                     /translation="MSFELKAGPTSLERLETAIARMLQKHGFVRVSADQPAFPAGLAA
                     LLLTDDPQRNLEVLDACVILPEALQALGADVARLVAGPEAAATLLQRFAVARAPAVVF
                     LRDGDYLGSLNGIRNWQEYRDEVARLAAGPARPRPISIPVLGAGQPGACA"
BASE COUNT          746 a         1432 c         1258 g          660 t
ORIGIN      
        1 ggcttccacc aacgcctgac gcagcagagg acgacaagac atgggtgctt acgaaacaca
       61 gggcttcagg ctcgacaatt ccggccgccg cgtggtggtc gacccggtga cccgcatcga
      121 gggccacatg cgctgcgagg tcaacctcga cagcaacaac gtgatccgca acgccgtctc
      181 caccggcacc atgtggcgcg gtctggaagt catcctcaag ggccgcgacc cgcgcgacgc
      241 ctgggccttc accgagcgca tctgcggcgt gtgtaccggc acccacgcgc tgacctcggt
      301 gcgcgccgtc gaggatgcgc tggcgatcaa gattccggag aacgccaaca ccatccgcaa
      361 cctgatgcag ttgaacctct acgttcatga ccatctggtg cacttctacc acctgcacgc
      421 gctcgactgg gtcgatgtcg tgtcggcact gaaggccgac ccgaaggcga cctcgacgct
      481 ggcccaaagc atttcctcgt ggccgctgtc gtcgcccggt tacttccgcg acatccagaa
      541 ccgcctgaag aagttcgtcg aatccggcca gctcgggccg ttcatgaacg gctactgggg
      601 caacccggcg tacaagctgc cgccggaagc caacctgatg gcggtcgccc actacctcga
      661 agcgctcgat ttccagaagg aaatcgtcaa ggtgcacacc atcttcggcg gcaagaaccc
      721 gcacccgaac tggctggtcg gcggcgtgcc ctgcgccatc aacctcgaag gcaccggcgc
      781 cgtcggcgcg gtgaacatgg agcgcctgaa cctggtcaag agcatcatcg accgctgcac
      841 cgagttcgtc gaacaggtct acatccccga tctgctcgcc atcggttcct tctacaaggg
      901 ctggctgcac ggcggcggcc tgtcgtcgaa gaacctgctc tcctacggcg acatcccgca
      961 gatcgccaac gactactcgg ccggcaacct gctgctgccg cgcggcgcca tcatcaacgg
     1021 caagctcgac gaaattcacc cgatcgacct caaggacccg gaacagatcc aggagttcgt
     1081 cgcccacagc tggtacaagt acccggacga aaccgtcggc ctgcacccct tcgatggcgt
     1141 caccgagccg aacttcgtgc tcggccccaa caccaagggg acgaagacca acatcaagga
     1201 actcgacgaa ggcgccaaat attcctggat caaggccccg cgctggcgcg gccacgccat
     1261 ggaagtcggc tgcctgtcgc gcatggtcct cggctacctg caaccgaagc agtacccgga
     1321 aatccacgcc ctggtcgacg gcgccctcaa gcatctcgac gtgccggtca ccgcgctgtt
     1381 ctcgaccctc ggccggaccg ccgcgcgcgg tctggaaacg gcgtactgcg tcaagctgca
     1441 gagccagcag ttcgacaagc tgatggccaa cctgaaggcc ggcgatctca ataccgccaa
     1501 catcgagaaa tgggaaccct cgacctggcc gaaggaggca caaggcgccg gcttcaccga
     1561 agccccgcgc ggcgcgctcg gccactggat caagatcaag gacaccaaga tcgacaacta
     1621 ccagtgcgtc gtgccgacca cctggaacgg cggtccgcgc gaccacaagg gccaaatcgg
     1681 cgccttcgaa gcgtcgctga tggacacccc ggtggccaag gccgacgaac cgctggaaat
     1741 cctgcgcacg ctgcattcct tcgatccctg cctggcctgc tcaacgcacg tgatgagccc
     1801 ggacggtcag gaaatgacgt cggtcaaggt ccgctgagcg gtccggaaag gaggcaatca
     1861 tgctttcaca acaggacatg ctggaggccg aacggcacgg tcggcaggtg cgttcgatct
     1921 acgtgtacga ggcgccggta cgcctctggc actgggtcaa cgcgctggcc atggtggtgc
     1981 tggcggtgac cggctacttc atcggcaagc ccctgccgac ggtccccggc gaagccgccg
     2041 agaacttcgt catgggctac atccgctttg cccatttcgc cgccgcctac atcttcgccg
     2101 tcggcctgct cggtcgcatc tactgggcgg cagtcggcaa ccaccacgcg cggcagatct
     2161 tcaagctgcc gatcaccaac ccgcaatggt ggagtgaagt cttcttcgaa ctgcgctggt
     2221 atcttttcct cgaaaagacg ccgaagaagt acgtcggcca caacccgatg gcgcagctga
     2281 tgatgttctt cttcttcacg gtgctggcgg tcggcatggt gttcaccggc ttcgcgctgt
     2341 acagcgaggg cgccgggctg ggcagttggc aggacgcgct gttcggctgg gtgatcccgg
     2401 cgctcggcgg ctcgctgcag gtgcataacc tgcaccggct gggcatgtgg atcatgatga
     2461 ccttcgccgt cgtccatatc tacgcggcga tccgcgagga catcatgagc cggcagtcgc
     2521 tgatttcgac catggtgtcg ggctggcgga tgttcaagga ctgagcgaag caggcttcac
     2581 cttgcgtagg cttgccccgg cgcactctgg tgcccggggc tttttcccca gaggaagaga
     2641 gatggaaaaa cgcatactcg tactcggcat cggcaacctg ctgtgggccg acgaaggttt
     2701 cggcgtgcgc tgcgtcgagg cgctcaacgc cagttggcag tttccgccgc aggtgacgct
     2761 gatggacggc ggcacgcagg gcctctacct gctgccctac gtccaggaag ccgactgcct
     2821 gctggtcttc gacgccgtcg attacggcga cacgccgggc gacctgcgtg aggtggtcgg
     2881 cgaccaggtg ccgcgtttca tgggcgccaa gaagatgagc ctacaccaga ccggcttcca
     2941 ggaagtgctg atggccgccg aactgaccgg caagctgccg gccgaactgg tgctcatcgg
     3001 cgtccagccg gaagagctgg aagactttgg cggcagcctg cgcgacgtgg tcaaggcgca
     3061 gatggcgccg gcgctcaacc tcgccctcga ctggctggaa cgctggggcg ccaccggccg
     3121 cctgcgcgac agcgaagccg aagcgatcac cgacgcggcg ctggccatcg acctctacga
     3181 aggcgagcgg ccgcccgccg accaggccta ccgcctgggc gacgcccgct tcctcaacct
     3241 ggaggccgcc tgatgtgcct cggcatcccg gcgcaggtca tcgaatgcgg cgagcactac
     3301 gcgcgctgcc aggggccaga cggtgaacag cgcatcgaca tccggctggt cggcgcccag
     3361 gcgccgggca cctggctgct ctgtttcctc gacgcggcgc gcgaggtgat cgacgccgaa
     3421 cgcgccaccg ccatcaccgc cgcccttgcc gcgctggacg cggtcaatgc tggagagacc
     3481 gatctttccg ccttcttcgc cgatctcgac cgcgaacccc aactgcccga attcctgagg
     3541 acgactcacc catgagtttc gaactgaaag ccggcccgac ctcgctcgaa cgcctggaaa
     3601 ccgccatcgc ccgcatgctg cagaagcacg gcttcgtccg cgtcagcgcc gaccagccgg
     3661 cttttccggc cggtctggcg gcgctgttgc tgaccgacga cccgcaacgc aacctggaag
     3721 tgctcgacgc ctgcgtcatc ctgccggaag cgctgcaggc actcggcgcc gacgtcgctc
     3781 gcctggtcgc cggcccggaa gccgccgcca cgctgctgca acgctttgcc gtggcgcgcg
     3841 ccccggccgt cgtcttcctg cgcgacggcg actatctcgg cagcctgaac ggcatccgta
     3901 actggcagga ataccgcgac gaagtcgccc gtctcgcggc cggcccggcc cggccgcgcc
     3961 cgatttccat cccggtgctc ggcgccggcc aacccggagc ctgcgcatga acccgtccac
     4021 cctgcgtccc ttcccgattt ccgtcgtcgc catggggccc ggctcgcaag gcgatgaagc
     4081 gcccgactac ctgccg
//
LOCUS       DAOSDX010000144         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7708_length_4093_cov_3.552501, whole genome shotgun sequence.
ACCESSION   DAOSDX010000144 DAOSDX010000000
VERSION     DAOSDX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7708_length_4093_cov_3.552501"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLN02_04280"
     CDS             <1..287
                     /locus_tag="PLN02_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOI51570.1"
                     /translation="GGMIFALADTAFAYACNSHNRNTVAAACHIDFLAPARQGETLEA
                     EAIERSAAGRTGVYDITVRNAAGTTIALFRGKSYRIAGEVIGGLQQSDRI"
     gene            521..1666
                     /locus_tag="PLN02_04285"
     CDS             521..1666
                     /locus_tag="PLN02_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI51571.1"
                     /translation="MKTSLKRIVAGAALACGALAAVAAEPIKIGSVLSVTGPAAFLGD
                     PELKTLQLYVAEINKKGGVLGRSLELVHYDDGSDAGKANGFTKRLITDDQVDILVGGT
                     TTGSTMSAAPLVDKAGIPFVSLAGAVVIIEPVKKFVFKTPHTDRMAAEKVFADMKKRG
                     LSKVALLSETSGFGASGKKETEAVAGKYGITLVANETYGPKDTDMSPQLTKIKNTAGV
                     QAVFVFGLGQGPAIVTKNYKQLGIALPLYQSHGVASDEFLKLAGPAAEGVRLPSPAQL
                     IPEKLPANDAQKPVVTAYANAYKAAYKTDVSTFGGYAYDGLMLAVDAIKRAGSTDKAK
                     VRDALEATKGFVGTSGTFNMSATDHMGLDLSAFRMLEVKNGDWTLVQ"
     gene            complement(1905..2825)
                     /gene="paaX"
                     /locus_tag="PLN02_04290"
     CDS             complement(1905..2825)
                     /gene="paaX"
                     /locus_tag="PLN02_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HOI51572.1"
                     /translation="MADFRQKRRVQAGSLIITAFGDAVLPRGGKIWLGSLIRLLEPLD
                     LNERLIRTSVFRLVKEEWLRTETLGRRADYALTAAGRRRFAEAARHIYASRAPLWDRR
                     WRLILVVGELPPKEREQVRNALLWQGFGVLGGECFVHPGAELSTVLDALLAEGLADAL
                     GALLPLFAADARSAASASDADLVARAWNLDALSGAYGHFVDRYQAVLDELQRDASLDL
                     ADQDAFLLRLLLIHDYRRMLLRDPELPEVLLPAQWPGQQARLLCKEIYKRLEAASSRH
                     LDAMLCVADGSVPVEDGTLARRFSQDDPLA"
     gene            3001..3831
                     /locus_tag="PLN02_04295"
     CDS             3001..3831
                     /locus_tag="PLN02_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOI51573.1"
                     /translation="MTQISFIKKLICIAFTLSSPLAPAALPEQHPVPGGIAVIDLGDS
                     EQPAPRAFLGKQRLAVVAEGGRWHVLVGIPLDTPPGTQAIRIETESGAAQREFIVAPW
                     TYGEQRLRLRNRRQVEPTPEDLRRIEREQAHTAAIKATFSTAAPDSGFTRPNLGRLSS
                     RFGLRRFFNGQERLPHSGLDFAAAAGSPVLAPAAGSVVDSGNYFFNGNTIFLDHGQGL
                     ISAYMHLSRIDVVVGQRVERGQRLGAVGASGRATGPHLHWTVILNATPVDPLLFLERR
                     "
     gene            complement(3841..>4093)
                     /locus_tag="PLN02_04300"
     CDS             complement(3841..>4093)
                     /locus_tag="PLN02_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3334 family protein"
                     /protein_id="HOI51574.1"
                     /translation="LSVDTNLDRPEARRVTFYTARNNIFYLELAIDHTEFIQINEFDA
                     QEIPDPDALIAEAGAASAVAPEAATGGSEHDDLLRSLGM"
BASE COUNT          803 a         1401 c         1261 g          628 t
ORIGIN      
        1 atggcgggat gatcttcgca ctcgccgaca cggctttcgc ctacgcctgc aacagccaca
       61 accggaatac cgtcgccgca gcctgtcata tcgatttcct tgcccccgcc cgccagggcg
      121 aaacgctcga agcggaggcc attgagcgtt cggcagccgg acgcaccggc gtttacgaca
      181 tcaccgtgcg gaacgccgcc ggcaccacca tcgccctctt ccggggcaag agctaccgca
      241 tcgccggcga ggtcatcggc ggactgcaac agtccgaccg gatctgaccc cagccaggca
      301 ggaagatcgt catgaccggc gcaatgacga ccggccatcc gttcggcatg agcgagactt
      361 ccccgatgcc gacggccact gacgcgtcaa aaatgacgca acaaacgcag tgccctgtgt
      421 accatgtgcg tcggcgtctg acaggacatc gcaatgatgc ggcgaacatg tcaaaacccg
      481 ctgtacacga caacaatcat ctcaaggagg agacccaccc atgaagacca gcctgaaacg
      541 aatcgttgcc ggagccgccc tggcttgtgg cgccctcgcg gctgttgccg ccgagccgat
      601 caagatcggc tccgtactct cggtcaccgg ccccgccgcc tttctcggcg acccggaact
      661 gaagacgctg caactctacg tcgccgagat caacaagaag ggtggcgtcc tcggccgctc
      721 gctcgaactg gtgcattacg acgacggcag cgatgccggc aaggccaacg gcttcaccaa
      781 gcggctgatc accgatgacc aggtcgacat cctggttggc ggcacgacca ccggttcgac
      841 catgtcggcc gccccgctgg tcgacaaggc cggcattccc ttcgtctcgc tggctggcgc
      901 cgtggtcatc atcgagccgg tcaaaaaatt cgtcttcaag acgccgcaca ccgaccgcat
      961 ggcggccgaa aaagtcttcg ccgacatgaa gaagcgcggc ctcagcaagg ttgccctgct
     1021 ctccgaaacc tccggctttg gcgcctccgg caagaaggaa accgaagcgg tggccggcaa
     1081 gtacggcatc acgctggtcg ccaacgaaac ctacgggccg aaggataccg acatgagccc
     1141 gcaactgacc aagatcaaga acacggccgg cgtccaggcg gtcttcgtgt tcggcctcgg
     1201 gcagggcccg gcgatcgtca ccaagaacta caagcaactc ggcattgccc tgccgctcta
     1261 ccagtcgcac ggtgtcgcct ccgacgaatt cctgaagctg gccggcccgg ccgccgaagg
     1321 cgtccgcctg ccgtcgccgg cccagctgat cccggaaaag ctgcccgcca acgacgccca
     1381 gaagccggtc gtcaccgcct acgccaacgc ctacaaggcg gcctacaaga ccgacgtatc
     1441 gaccttcggc ggctacgcct acgacggcct gatgctggcg gtcgacgcga tcaagcgcgc
     1501 cggcagcacc gacaaggcca aggtgcgcga cgcgctcgaa gcgaccaagg gcttcgtcgg
     1561 caccagcggc accttcaaca tgtcggcgac cgaccacatg gggctcgacc tctccgcctt
     1621 ccgcatgctg gaagtgaaga acggcgactg gactctggtt cagtaaggca ttcctcaccc
     1681 actgcttgtt gactgcactt caggccgccc ggaaccgggc ggccttttct tttgccgatt
     1741 ctttgatctg cccccaggag ctcggcgaca tccagggcaa ggtgggtcag atccgtggca
     1801 cgccagggtc ggggcgacga agggttttcc gcccccgggg caaagaaaca cccagggtca
     1861 tgccaccgtt caggcgccat cgggaggcgc tccgacggcc aacgtcaggc gagcggatcg
     1921 tcctggctga agcggcgggc cagcgtgccg tcctcgaccg gcacgctgcc gtcggcaacg
     1981 cacagcatgg catcgaggtg acgcgacgag gcggcttcca gccgcttgta gatttccttg
     2041 cacaggagcc gcgcctgctg ccccggccac tgcgccggca gcagcacttc cggcagttcc
     2101 gggtcgcgca gcagcatgcg ccggtaatcg tggatcaaca gcagacggag caggaaggcg
     2161 tcctgatcgg ccagatcgag cgaggcatcg cgctgcagtt catcgagtac cgcctggtaa
     2221 cgatcgacga aatggccgta agccccggac aaggcgtcga gattccaggc ccgagccacc
     2281 agatcggcgt cgctggccga cgccgccgag cgggcatcgg cagcgaacag cggcagcagc
     2341 gcaccgagcg cgtcggccag gccttcggcg agcagcgcgt cgagcacggt cgacaactcc
     2401 gccccggggt gcacgaaaca ttcgccgccg agcacaccga agccctgcca gagcaaggca
     2461 ttgcgcacct gctcgcgctc cttcggcggc agctcgccga ccaccaggat caggcgccag
     2521 cggcggtccc agagcggcgc ccgcgacgca tagatgtggc gggccgcttc cgcgaagcgc
     2581 cggcgaccgg ccgcggtcag cgcgtaatcg gcgcgccggc cgagggtttc ggtgcgcagc
     2641 cactcttcct tgaccaagcg aaaaaccgaa gtgcggatca ggcgctcgtt gaggtcgagc
     2701 ggttcgagca gacgaatcag gctgcccagc cagatcttgc cgccgcgcgg cagcacggcg
     2761 tcaccgaagg cggtgatgat cagcgaaccg gcctgaacgc gacgtttttg ccggaaatcg
     2821 gccaaccttt gttgtattgc attgcccaca gaatcataat cctgtcaaag cagcggcaaa
     2881 aaaagccgga actggattgg gaattgccca tcttatccga tccatgccca tcattccaaa
     2941 tgactttttg gaaaccctgg caatagataa gccgccacaa ccaaaaacca gaacagagac
     3001 gtgacacaaa tttcattcat caaaaaatta atctgtattg cttttaccct cagcagcccg
     3061 ctcgcgccgg cagcactacc ggaacaacac ccggttcccg gcggcatcgc cgtcatcgac
     3121 ctcggcgaca gcgaacagcc ggcgccgcgc gccttcctgg gcaaacaacg gctggcggtg
     3181 gtcgccgagg gtgggcgctg gcacgtgctg gtcggcattc cgctcgacac gccgccggga
     3241 acgcaggcga tccgcatcga aaccgaaagc ggcgctgcgc agcgggaatt catcgtcgcc
     3301 ccctggactt acggcgaaca acggctgcgc ctgcgcaacc ggcgccaggt cgaaccgacg
     3361 cctgaggatc tgcgtcgcat cgaacgcgaa caggcgcaca cagcggcaat caaggcgact
     3421 ttcagcaccg ccgcgccgga cagcgggttt acccggccga acctgggacg tctgtcgtcg
     3481 cgcttcggcc tgcgccgctt cttcaatggc caagagcgcc tgccgcacag cggcctcgat
     3541 ttcgccgccg ccgccggcag cccggtgctg gcgccggcgg caggcagcgt ggtggacagc
     3601 ggcaattatt tcttcaacgg caacaccatc ttcctcgacc acggccaggg gctgatcagc
     3661 gcttacatgc acctgtcgcg catcgacgtc gtggtcggcc agcgcgtcga gcgtggccag
     3721 cggctgggcg cggttggcgc cagcggccgc gccaccggac cgcacctgca ctggacggta
     3781 atcctcaacg cgacgccggt cgacccgctg ctctttctcg aacgccgctg agccggcgta
     3841 tcacatgccg agcgagcgca gcagatcgtc gtgctcgctg ccccccgtcg cggcttccgg
     3901 cgccacggcc gaggcagcgc cggcttcggc gatcagggca tccggatcgg gaatttcctg
     3961 ggcgtcgaac tcgttgatct ggatgaactc ggtgtggtcg atcgccagtt cgaggtagaa
     4021 gatgttgttg cgcgcggtat agaaggtgac gcggcgcgct tccggtcggt cgaggttggt
     4081 atcgacgctg agg
//
LOCUS       DAOSDX010000145         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7749_length_4077_cov_3.077573, whole genome shotgun sequence.
ACCESSION   DAOSDX010000145 DAOSDX010000000
VERSION     DAOSDX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7749_length_4077_cov_3.077573"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLN02_04305"
     CDS             <1..720
                     /locus_tag="PLN02_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOI51575.1"
                     /translation="VPGDIAGNRARIEAGIRDAVRHGADWVVTPELAETGYNFTKRIG
                     SDWVHPFPSNWLHGLTAVARDNGVALFVGVAERDPATQKLHNSVAVIDRSGKLLGSYR
                     KQRPVGGAEAWSVAGGEGQPFLVDGLRVGTLICADAWRPEPAAQLAARGAQILLSPAN
                     WPPVEGMGPGDVWEKRSRQTGLPLVVVNRGGKEPELDFSRGESAVAIDGRRVFTFTAA
                     EGGIFYVDWDRHRRFRSVRTD"
     gene            737..1864
                     /locus_tag="PLN02_04310"
     CDS             737..1864
                     /locus_tag="PLN02_04310"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HOI51576.1"
                     /translation="MNAAYRFDDWETPAAAPQNPAVDAKIAPKIEVDRSILPAAALAP
                     INAADKRIVNGKADINQLAPFKYPWAWEFFLKANRNHWTPLEISMAQDVHDYHHKLSL
                     AERHVFENVLAYLTTSDILAMRNIGLAVMEKMSAPELQIYQARQVYEEALHTWTYQHC
                     IESIGLDQQEIYNRYRVVPQIHGKIALANRRLEKVLRSDFDLTDRANLHEFALSYFFF
                     AVIFEGCWFYNGFTPIFALQRRGLMKGAAEQLQYIMRDEVLHCAFGIRVVRELLKEEN
                     LTIDPQALRQLWDEAEAAEHAYAGYILRDPILGYNAELHVQQFRFIANRRARQVGVDE
                     PFPGAENVLPWLDEQANLRKEKNFFETRVTEYQTGGALAWD"
     gene            2091..>4077
                     /locus_tag="PLN02_04315"
     CDS             2091..>4077
                     /locus_tag="PLN02_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HOI51577.1"
                     /translation="MHVKFTRKPLVAALMLAGLGGAWAQEAERQLSEISVVGRGEGQA
                     YYHEESGVARSETPLRELPQSVRVIPRQLIDDIGAVRLEEAYDYVSGVTRQNSFGGLW
                     DNFAIRGFAGNENTGPNFLRNGFAGNRGYNAPRDMANVERIEVLKGPSAALYGASEPG
                     GILNVVTKKPQFKAGNSLEIYAGSYDSYRTSFDSTGPLSDNLAYRLNVAVEDKGSFRD
                     HIDSQRYLVAPSFTWVLSPNTLVNYDLELLRHKAPMDRGVVAVGGRTGSISRETFLGE
                     PGDGDITIDNRTHQLTLEHEFSSDWKAKAGLAYRTTDLAGYSTEVRPSNPLLFVPPGS
                     RNVNRERRYRNYDSEDVSFQGEVHGKFSTGSLKHELLTGLDSYRFSLDSVMMRVRPAD
                     YSIDIDNPVYGQPLPTPTANTDTLEKQRATGVFMQDQIHLAEDWRLLLGVRHDRFHQT
                     IDNRRTGLRSKQSEGKTTPRVGLTWLLSPTVSLYALASESFRPNTGSSFAGAAFAPEE
                     GRAKEVGIKYESADKRYGGTLSLFNIDKKNVLTNDPANAGFSLAAGEARSRGVEIDVS
                     GQLTAKLRVTANYAYTDAEITKDNNAALVGARLLNVPKHSGSVLAMYEDGFAAGRYGV
                     GAGATHVGKRAGNALATFDLPAYTTARLMAYWKPSQNL"
BASE COUNT          779 a         1439 c         1226 g          633 t
ORIGIN      
        1 gtgcccggcg acatcgccgg caaccgcgcc cgcatcgaag ccggcatccg cgacgcggtg
       61 cgccacggcg ccgactgggt ggtgacgccg gaactggccg aaaccggcta caacttcacc
      121 aaacgcatcg gcagcgactg ggtgcatccc ttccccagca actggctgca cggcctcacc
      181 gccgtcgccc gcgacaacgg cgtcgcgctc ttcgtcggcg ttgccgagcg cgacccggcg
      241 acgcagaagc tgcacaacag cgtcgccgtc atcgaccgca gcggcaagtt gctcggcagc
      301 taccgcaagc agcgcccggt cggcggcgcc gaagcctggt cggtggccgg cggggaaggg
      361 cagcctttcc tggtcgatgg cctgcgcgtc ggcaccctga tctgcgccga cgcctggcgc
      421 ccggaaccgg ccgcccagct tgccgcacgc ggcgcgcaaa tcctgctctc gccggccaac
      481 tggccgccgg tcgaaggcat ggggccgggc gacgtctggg aaaaacgctc gcgccagacc
      541 gggctgccgc tggtcgtcgt caatcgcggc ggcaaggagc cggaactcga tttctcgcgc
      601 ggcgagagcg ccgtcgccat cgacggccgc cgcgtcttca ccttcaccgc cgccgagggc
      661 ggcattttct acgtggactg ggatcgccac cggcgcttcc ggtccgtccg caccgactga
      721 ccacgaggtt catgccatga atgctgccta ccgcttcgac gactgggaaa cccccgctgc
      781 cgcaccgcaa aatcctgcgg tcgacgccaa aattgcgcca aaaatcgagg tcgaccgcag
      841 cattttgccc gccgccgcac tggcgccgat caacgccgcc gacaagcgca tcgtcaatgg
      901 caaggccgac atcaaccagc tggcgccgtt caagtacccc tgggcctggg agttcttcct
      961 caaggccaac cgcaatcact ggacgccgct cgaaatcagc atggcgcagg acgtgcacga
     1021 ctaccaccac aagctcagcc tggccgagcg ccacgttttc gagaacgtgc tcgcctacct
     1081 gaccacctcc gacatcctgg ccatgcgcaa catcggcctg gcggtgatgg agaagatgag
     1141 cgcgcccgaa ctgcagatct accaggcccg ccaggtttat gaggaagcct tgcacacctg
     1201 gacctaccag cactgcatcg agagcatcgg cctcgaccag caggaaatct acaaccgtta
     1261 ccgcgtcgtg ccgcagatcc acggcaagat cgcgctcgcc aaccgccggc tggaaaaggt
     1321 gctgcgttcg gacttcgacc tcaccgaccg cgccaacctg cacgaattcg cgctctccta
     1381 cttcttcttc gcggtgatct tcgaaggctg ctggttctac aacggcttca cgcccatctt
     1441 cgcactgcaa cggcgcggcc tgatgaaggg cgccgccgag caactgcaat acatcatgcg
     1501 tgacgaagtc ctgcactgcg ccttcggcat ccgcgtcgtg cgcgaactgt tgaaggaaga
     1561 aaacctgacg atcgacccgc aggcgctacg ccaattgtgg gacgaagccg aagccgccga
     1621 acacgcctac gccggctaca tcctgcgcga ccccatcctc ggctacaacg ccgagctgca
     1681 cgtccagcaa ttccgtttca tcgccaaccg gcgggcgcgc caggtcggcg tcgacgaacc
     1741 cttccccggc gccgagaacg tcctgccctg gctggacgaa caggccaacc tgcgcaagga
     1801 gaagaacttc ttcgagacgc gggtcaccga gtaccagacc ggcggggcgc tggcctggga
     1861 ttgaggttcc gggcggcagt ctgctttcca cactggtagt gcactgaccg gcgaaggtcg
     1921 gtcgggtgcg cgctcggcga cgataaatcg cgggcagggc gcctggcacg gaaaatgtat
     1981 gaagtaattt attttcatca tacttgatgc gataatctcc gcatcggtaa tgctatacag
     2041 tcgcatttgc gatgttataa catcacgttt tttgccatcc ggaaattgca atgcacgtca
     2101 aattcacccg caaacccctg gtcgccgccc tgatgctggc cggtcttggc ggcgcctggg
     2161 cccaggaggc cgagcgtcag ctgtcggaaa tcagtgtcgt cggccgcggc gaaggccagg
     2221 cttactatca tgaagagagc ggcgtcgccc gctcggaaac gccgctgcgc gaactgccgc
     2281 agtcggtgcg ggtgattccc cggcagctga tcgacgacat cggcgccgtt cgcctcgagg
     2341 aagcctacga ctacgtcagc ggcgtcaccc gccagaacag cttcggcggc ttgtgggaca
     2401 acttcgccat ccgcggcttc gccggcaacg agaacaccgg accgaacttc ctgcgcaacg
     2461 gcttcgccgg caaccgcggc tacaacgccc cgcgcgacat ggccaacgtc gagcgcatcg
     2521 aagtcctcaa ggggccgtcg gcggcgctct acggcgccag cgaaccgggc ggcatcctca
     2581 acgtggtgac caagaagccg cagttcaagg ccggcaattc gctggaaatc tatgccggca
     2641 gctacgacag ctaccgcacc tctttcgatt cgaccggtcc gctgagcgac aacctggcct
     2701 accgcctgaa cgtcgccgtc gaggacaagg gcagcttccg tgaccacatc gacagccagc
     2761 gttacctggt ggcgccgtcc ttcacctggg tgctgtcgcc gaacacgctc gttaattacg
     2821 acctcgaact gctgcgccac aaggcgccga tggatcgcgg cgtggtcgcg gtcggcggcc
     2881 gcaccggcag catctcgcgc gaaaccttcc tcggcgaacc gggtgacggc gacatcacca
     2941 tcgacaaccg gacccaccag ctgacgctcg agcacgagtt ctccagcgac tggaaggcca
     3001 aggccgggct ggcttaccgg acgaccgacc tggccggcta ttcgaccgaa gtgcggccga
     3061 gcaatccgct gctcttcgtg ccgcccggct cgcgcaacgt caatcgcgag cgtcgctacc
     3121 gcaactacga ctccgaggat gtctccttcc agggcgaggt gcacggcaag ttctccaccg
     3181 gcagcctcaa gcacgaactg ctgaccggcc ttgacagcta tcgcttctcg ctcgactcgg
     3241 tgatgatgcg ggtgcgtccg gccgattact cgatcgacat cgacaacccg gtctacggcc
     3301 agccgctgcc gacgccgacg gcgaacaccg atacgctgga aaagcagcgc gccaccggcg
     3361 tcttcatgca ggaccagatc cacctcgcgg aagactggcg cctgctgctc ggcgtccgtc
     3421 acgaccgctt ccaccagacc atcgacaacc gtcgcaccgg cctgcgcagc aagcagtctg
     3481 aaggcaagac gacgccgcgc gtcggcctga cctggctgct cagcccgacc gtctcgctct
     3541 atgccctggc cagcgaatcg ttccggccga acaccggcag cagcttcgcc ggtgccgcgt
     3601 ttgcgccgga agaagggcgg gccaaggaag tcgggatcaa gtacgaatcg gccgacaagc
     3661 gctacggcgg cacgctttcg ctcttcaaca tcgacaagaa gaatgtgctg accaacgatc
     3721 cggccaacgc cggcttctcg ctggcggccg gcgaggcgcg cagccgcggc gtggaaatcg
     3781 acgtttccgg gcagttgacc gcaaaactcc gggtcaccgc caactacgcc tacaccgacg
     3841 ccgagatcac caaggacaac aacgcggcgc tggtcggcgc ccgcctgctc aacgtgccca
     3901 agcacagcgg cagcgtcctg gcgatgtacg aggacggctt cgccgccggt cgctatggcg
     3961 tcggcgctgg cgccacccat gtcggcaagc gcgccggcaa cgcgcttgcc accttcgatc
     4021 tgccggccta cacgacggcg cgcctgatgg cctactggaa accgagccag aacctgc
//
LOCUS       DAOSDX010000146         4075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7757_length_4075_cov_2.814179, whole genome shotgun sequence.
ACCESSION   DAOSDX010000146 DAOSDX010000000
VERSION     DAOSDX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7757_length_4075_cov_2.814179"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..2405)
                     /locus_tag="PLN02_04320"
     CDS             complement(120..2405)
                     /locus_tag="PLN02_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOI51578.1"
                     /translation="MNKVSALAADFAAEFGAAPWAALAGIWHDLGKYREGFQRYIRQC
                     GDPDAHIEGRVAGPEKTHSAAGALWAQQYLTEVDKRSGPVVARVLAYLIAGHHAGLDN
                     WFGGLHERFNREDTIREKCDALAVAIPEAILKPSAGLPDLNAIRTDQKDNIPGRFALW
                     VRMLFSCLVDADFLDTERFMSQGKAEARSGFMPVDELEKRLAERLTLMAQEVAARGEA
                     GSQVNLKRAEVLRACLAKAELPPGVFSLTVPTGGGKTLSSLAFALRHAVRHGKRRVIY
                     AIPYTSIIEQTAGIFRSIFGDENVIEHHSNVEVDDKQETARSRLACETWDAPLIVTTN
                     VQLLESLFASRTSRCRKLHNLVGSVIVLDEAQLLPVPYLQPVVDVLRLLVKDYGVTLV
                     LCTATQPTLESRNGFDQARQLRGFAAGEIREIVDDVTGLYGALERVRVHLPADLKTPC
                     NWEQLAPQIAGHDAVLAVVGRRADARELYTQVKAEDRTGLWHLSGLMCAQHRSDTIAD
                     IKQALLARRQALATGQTPPPIRVISTQLVEAGVDIDFPVVYRALAGLDSIAQAAGRCN
                     REGRLAKGEVHVFVPPKPAPPGLLRMAEQATRILWEALPPEADPMGVERFADFFRRLY
                     GDADLDAKDVCNLLRVGQIGDVNFRTAAERFRLIDEAEGATVFVRYKRNADDEAIDVL
                     LNTLKKEGPQRWLLRKLQRYGVSIYQRDLQRLEGLGDIEPLGGDFPGLYVQSFGNDVL
                     YDRVLGINVDGAPGDPGELVK"
     gene            complement(2522..3517)
                     /locus_tag="PLN02_04325"
     CDS             complement(2522..3517)
                     /locus_tag="PLN02_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOI51579.1"
                     /translation="MAKTERYQHIRSMLGARRSVSLRELMSHFEVSRATIFRDIAFLR
                     NRLGMPVDFDRELGAYRLLAPPGSAELPGVWLSADEIHALLSMQQLLENIDASGILRD
                     QLQPLRQRLLGMLGAGSHTAEEIARRIRIIAATPRQCHAASFQTVADALLARRRLEIR
                     YRARASAATTSREVSPQRLVHYRDNWYLDAWCHTRQALRSFAVDAIEKAKTLATPAQD
                     IADSELDRQLAAGYGIFAGSDVRWARLRFSAMRARWVAGEQWHPQQQGQLLPDGRYEL
                     RLPYSADPELLMDILKYGPDCEVLEPEALRQRVIGLLEETRGMYRGVADGETGGG"
     gene            complement(3654..>4075)
                     /locus_tag="PLN02_04330"
     CDS             complement(3654..>4075)
                     /locus_tag="PLN02_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOI51580.1"
                     /translation="PEVQPMARALVQKAALHGISIKVLSGLRSYAEQDALYAKGRTAP
                     GPKVTNARAGYSNHNFGIAFDIGVFEGARYLADSPKYKAVGALGMDLGLEWGGSWKTI
                     VDQPHYQLRPAWAADLRERDMLAELRARTSAGRPIYA"
BASE COUNT          657 a         1261 c         1386 g          771 t
ORIGIN      
        1 agcatggctt ctcgctggtg ttccagcgtg gattgaaact caatggtgaa agttgtccgt
       61 tgcatccgta acgctgggcc gggcttgtcc cggccctttc attttggtct tcgtccgtat
      121 cacttcacca attcccccgg atcgcccggc gcaccatcca cattgatacc cagcacccgg
      181 tcgtaaagca catcgttgcc gaacgactgc acgtacagac ccggaaaatc gccgccgagc
      241 ggttcaatat cgcccagccc ttccagacgt tgcaggtcgc gctggtagat gctcacgccg
      301 tagcgttgca gcttgcgtag cagccagcgc tgcgggcctt cctttttcag ggtgttcagc
      361 aacacgtcga tggcttcatc atccgcgttg cgcttgtagc ggacgaagac cgtggcgcct
      421 tcggcttcgt cgatcaggcg gaagcgctcg gcggcggtgc ggaaattgac gtcgccgatc
      481 tggccgacgc gcagcaaatt acagacatct ttcgcatcga gatcggcgtc accgtagagc
      541 cggcggaaaa agtccgcgaa gcgctcgacg cccatcgggt cggcctcggg cggcaaggct
      601 tcccacagga tgcgcgtcgc ctgttcggcc atgcgcaaca gccccggcgg cgccggtttc
      661 ggcggcacga agacatgcac ttcgcccttg gccagccgcc cctcacggtt gcagcggccg
      721 gcagcctggg cgatggagtc cagcccggcc agggcgcgat agacgacggg gaagtcgata
      781 tcgacgccgg cttcgaccag ttgggtgctg atcacccgga tcggcggcgg ggtttgcccg
      841 gtcgccagtg cctgccgcct ggccagcaag gcctgcttga tgtcggcaat ggtgtcgcta
      901 cggtgctggg cgcacatcag gccggacaaa tgccacagtc cggtgcggtc ttcggccttg
      961 acctgcgtat agagttcgcg cgcatcggcg cggcggccga caacggcgag cacggcatcg
     1021 tgcccggcga tttgtggcgc cagttgctcc cagttgcagg gggttttcag gtcggccggc
     1081 agatggactc tgacccgctc cagcgcgccg tacagcccgg tcacatcatc gacgatttca
     1141 cggatttcac cggccgcgaa gccgcgcagt tggcgggcct gatcgaaacc gttgcggctt
     1201 tccagcgtcg gctgggtggc ggtgcacagc accagcgtta cgccgtaatc cttgaccagc
     1261 aggcgcagca cgtcgaccac cggttgcaga tagggcaccg gcagcaattg cgcctcgtca
     1321 agcacgatga cgctgccgac cagattgtgc agcttgcggc agcgcgaggt gcggctggcg
     1381 aacaggcttt ccagcaattg cacgttggtc gtgacgatca acggcgcatc ccaggtttcg
     1441 caggccaggc gggaacgggc ggtttcctgc ttgtcgtcca cctcgacatt gctgtggtgc
     1501 tcgatgacgt tttcgtcgcc gaagatgctg cggaagatgc cggccgtctg ctcgatgatg
     1561 ctggtgtagg gaatcgcgta gatgacccgg cgtttgccat ggcgcaccgc atggcgcagc
     1621 gcgaaagcca ggctggacag cgtcttgccg ccgccggtcg gcaccgtcag gctgaacacg
     1681 ccgggcggca actcggcctt ggcgaggcag gcgcgcagca cttcggcgcg tttcagattg
     1741 acctggctcc cggcttcgcc gcgcgctgca acctcctgcg ccatcaacgt cagacgttcg
     1801 gccaaacgct tttccagctc gtcgacgggc atgaaaccgc tacgcgcctc ggccttgcct
     1861 tgcgacatga atcgctcggt gtcgagaaaa tcggcatcga ccaggcagga aaacagcatg
     1921 cgtacccaca gcgcaaaacg accgggaatg ttgtctttct gatcggtgcg gatcgcgttc
     1981 aggtcaggca agccagccga gggcttgagg atagcctcag gaatcgccac ggccaacgca
     2041 tcgcactttt ctctaatcgt atcttcgcga ttaaagcgtt cgtgcaggcc gccgaaccag
     2101 ttgtccagtc cggcgtggtg accggcgatc agataggcca gcacacgcgc caccaccggg
     2161 ccggaacgtt tgtctacttc ggtcagatat tgctgcgccc acaaggcgcc ggcagccgag
     2221 tgcgtcttct ccggaccggc gacgcggcct tcgatgtgcg catcgggatc gccacactgc
     2281 cggatgtagc gctggaagcc ctcccggtat ttgcccaggt cgtgccagat gccagccagc
     2341 gccgcccacg gcgcagcgcc gaattcggcg gcgaaatcag cagccagggc ggacaccttg
     2401 ttcaagtgct catccagact gtgccactgc aaatgcagcc catcgcttct gacatgcgct
     2461 acccattgcg gctgttcgtc atcttccaag gcgttctcct ttcctgctgt tgatcgctag
     2521 cctacccgcc cccagtctca ccatctgcga ctccccgata catccctcgc gtttcctcca
     2581 gcagcccgat cacccgctgg cgcaaagctt cgggttcgag gacttcgcaa tccgggccgt
     2641 atttgaggat gtccatgagc agttccgggt cggcgctgta gggcaggcgc agttcgtagc
     2701 ggccgtcggg gagaagttgg ccttgctgtt gcgggtgcca ttgttcgccg gccacccagc
     2761 gggcgcgcat ggcggagaag cgtaggcgcg cccagcgcac gtcgctgccg gcgaagatgc
     2821 cgtagccggc ggcgagttgg cggtcgagtt cgctgtcggc gatgtcctgg gccggggtgg
     2881 cgagggtttt tgccttttcg atggcgtcga cggcgaagct gcgcagtgct tgccgggtat
     2941 ggcaccaggc gtcgagatac cagttgtcgc ggtagtggac gaggcgctgc ggggagactt
     3001 cgcggctcgt ggtggcggcg ctggctcggg cgcggtagcg gatttccagg cggcgacggg
     3061 cgagcagcgc gtcggcaacg gtctggaagc tggcggcgtg gcattggcgc ggggtggcgg
     3121 cgatgatgcg gatacgccgg gcgatttcct cggcggtgtg gctgccggcg ccgagcatgc
     3181 cgagcaggcg ctggcgcagg ggttgtagct ggtcgcgcag gatgccgctg gcgtcgatgt
     3241 tttccagcag ttgctgcatg ctgagcaggg catggatttc gtcggcgctg agccagacac
     3301 cgggcagttc ggcgctgcct ggcggggcga gcagacgata ggcgccaagc tcgcggtcga
     3361 agtcgaccgg catgccgagg cggttacgga ggaaggcgat gtcgcggaag atggtggccc
     3421 gcgaaacttc gaagtgggac atcagttcgc gcaggctgac gctgcggcgg gcgccgagca
     3481 tgctgcggat gtgttggtag cgctcggttt tcgccaagtt tcgcccctca ggccaggatc
     3541 gatttgcatc gatcataacc gctcgtcggc cgggactcga tcagacgatg ctcatgtcgg
     3601 ccgccgggca ggccctggtc agccgggagc gatcccggct gtcttgttgt gcatcaggcg
     3661 tagatcggcc gcccggcgct ggtgcgggca cgcagttcgg ccagcatgtc gcgttcgcgc
     3721 agatcggcgg cccaggctgg gcgtagctgg tagtgcggct ggtcgacgat ggtcttccag
     3781 ctgccgcccc attccaggcc gagatccatg ccaagggcgc cgaccgcctt gtatttgggg
     3841 gaatcggcca ggtagcgggc accttcgaag acgccgatgt cgaaggcgat gccgaagttg
     3901 tggttggagt agccggcgcg ggcgttggtg actttcgggc caggggcggt gcggcccttg
     3961 gcgtaaaggg cgtcctgttc ggcgtagctg cgcaggccgc tgagcacctt gatgctgatg
     4021 ccgtgcagcg cggccttctg taccagggcg cgggccatcg gctggacttc gggga
//
LOCUS       DAOSDX010000147         4068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7772_length_4068_cov_3.743334, whole genome shotgun sequence.
ACCESSION   DAOSDX010000147 DAOSDX010000000
VERSION     DAOSDX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7772_length_4068_cov_3.743334"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLN02_04335"
     CDS             complement(<1..358)
                     /locus_tag="PLN02_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOI51581.1"
                     /translation="MELILISGLSGSGKSVALNLLEDSGYYCVDNLPVVILTVLVGML
                     QEEQVSKVAVAIDARSGGGIELLPGKLKRLSEDGVQVTFLFLHANEETLLKRYSESRR
                     RHPLADDERSLAESIRL"
     gene            527..1705
                     /locus_tag="PLN02_04340"
     CDS             527..1705
                     /locus_tag="PLN02_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOI51582.1"
                     /translation="MTPTLPLPELPFLAELTALRRDIHAHPELAFDERRTADIVAAEL
                     ARYGLEVHRGLARTGVVGVLRAGSSPRMIGLRADMDALPLAELNEFPHHSKHPGKMHA
                     CGHDGHTAMLLGAARYLAENPDFDGSVVFIFQPAEESEGGAAVMIEDGLFEKFPVEAV
                     FGLHNWPGIPVGEMMAMPGPVMAGTCGFEIHVRGHGCHAAMPHQGIDAIVTGAQLVQA
                     LQTVVSRTLHPCDAAVVSVTQFHAGEAWNIIPEEVVMRGTIRSFKPEVQESIERAIER
                     LCSGIAAANGAQISVRFEHRYPPTVNSPAEARFCQQVAAEVFGAERVLTDILPSMGAE
                     DFAYMLREKPGCYVWLGNGPGTGGCTLHNPHYDFNDQLLTLGVAYWAQLVRRALPRAA
                     "
     gene            complement(1709..3322)
                     /locus_tag="PLN02_04345"
     CDS             complement(1709..3322)
                     /locus_tag="PLN02_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI51583.1"
                     /translation="MSFRNRLLLGMALIMAAFVSAVGVAYQGMLGTSASFGRFLDGPS
                     ALRQDYREMYAQGLQMGQALRNITLDPENPKAYQNLDKAREDFAKARQSAVVNAAKVE
                     GFTETIGGLEKLAEAQASAQKEVLAALKEGRFDEAKALINAKETPAWRKLKQVLLDDL
                     DILKQKTLEERQRVEDAAHNQEMLTLVLTAIAILIGIGSVWTTLAYVRRELGGEPAYA
                     REVASAVATGDLSRTIALDGSNGASLLGALARMQDQLRQLVGSLASHAREVEQGARLV
                     DDATRQVAAGSQQQLNYAQEMVSNVHVATSGLNDIMRAVSEAERIVGDSGATSDSGAA
                     LASKAAAETAEMAGSVQTTATHIRELGAQSAKISSILGVISDIAGQTNLLALNAAIEA
                     ARAGEQGRGFAVVADEVRKLAERTTQSTSEISTMVASIQSGTQRAVEGMEAGLLQVGE
                     SVELSRQSREAFDRMKAGSQEVSTVVGRIAQAISAENDSEQKIEALVEQVRNLIAQND
                     QALRQVVSSADRLNTVSSELNQAIARFRL"
     gene            complement(3381..>4068)
                     /locus_tag="PLN02_04350"
     CDS             complement(3381..>4068)
                     /locus_tag="PLN02_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI51584.1"
                     /translation="QAAFGCSIGVPAGLQASIAEWDEDALLLSPLGQQAAPFRHDALI
                     EAGSEIEFAGLNWQALAVPGHDMEALAFYNPERRLLISGDALWENGFGVVFPELLGTA
                     DGLTATRATLEMLGRLPIDIVIPGHGAPFTDVDRALATAFRRLDGYCADIERLAWHAL
                     KVIVAFALMERQQLPAAGFADFLASLPFAVDINQRYLALAEDELAARLLADLSGAGVL
                     VHREDMLILR"
BASE COUNT          667 a         1306 c         1339 g          756 t
ORIGIN      
        1 ccaggcggat cgattcggcc agcgagcgtt cgtcgtcggc cagcggatgg cggcgtcgcg
       61 attcggaata gcgcttgagc agcgtttcct cgttggcgtg caggaacagg aaggtgacct
      121 gcacaccatc ctcgctcagt cgcttgagtt tgccaggcag cagttcaatg ccgccgccgg
      181 agcgggcatc gatggcaacc gccaccttgc tgacctgttc ttcctgcagc atgccgacca
      241 gcacggtcag aatgacaaca ggcaagttgt cgacgcagta atagcccgag tcttcaagca
      301 ggttgagggc gacggacttg ccggagccgg aaagtccgct gatcagtatg agttccatgg
      361 tcggcaggat aaaggaaggc gccgccgcgc cgcaaccttt aacagttgtc cttaggcgat
      421 tggcatgttg ccgtgcggcc gcggccctcg accctgaaag gcagggcgtt tttccgggag
      481 tgtttaaagg ataataccgg gagaagaaaa cccgtggaga gacaccatga ccccgaccct
      541 tcccctgccc gagttgcctt tcctggctga actgacagcg ctgcgccgcg acatccatgc
      601 ccaccccgaa ctggctttcg acgagcgccg gacggccgat atcgtcgccg ccgaactggc
      661 ccgctatggg ctcgaagtgc atcgtggcct ggccaggacc ggcgttgtcg gcgtcctccg
      721 ggctggtagt tcgccgcgca tgatcggcct gcgcgccgac atggacgcgc tgccgctcgc
      781 cgagctgaac gaatttccgc atcactcgaa gcaccccggc aagatgcacg cctgcggcca
      841 cgacggccat accgcgatgc tgctgggtgc cgcccgttac ctggcggaga acccggattt
      901 cgacggtagc gtcgtcttca tcttccagcc ggctgaggaa tcggaaggcg gcgccgcggt
      961 gatgatcgag gacggcctgt tcgagaaatt cccggtcgag gcggtgttcg gcctgcacaa
     1021 ctggccaggc atcccggtcg gcgagatgat ggcgatgccg gggccggtga tggccggcac
     1081 ctgcggtttc gagattcacg tgcgcggcca cggctgccac gcggcgatgc cgcaccaagg
     1141 gatcgacgcc atcgtcaccg gcgcgcaact ggtgcaggcg ctgcagaccg tggtcagccg
     1201 caccttgcat ccctgtgatg cggcggtggt cagtgtcacc cagttccacg ctggcgaggc
     1261 ctggaacatc attccggaag aagtggtgat gcgcggcacc atccgcagct tcaagccgga
     1321 agtccaggag agcatcgagc gcgccattga gcggctgtgc agcggcatcg ccgccgccaa
     1381 cggggcgcag atttccgttc gtttcgagca ccgctatccg ccgacggtca acagcccggc
     1441 cgaggccaga ttctgccagc aggtcgccgc cgaggtgttc ggcgccgagc gggtgctgac
     1501 cgacatcctg ccgtcgatgg gcgccgagga tttcgcctac atgctgcgcg agaaacccgg
     1561 ctgctacgtc tggctgggca acggcccggg cactggcggc tgcacgctgc acaatccgca
     1621 ctacgacttc aacgaccagt tgctgaccct gggcgtcgcc tactgggcac aactggtcag
     1681 gcgcgcgctg cccagggcgg cctgaggctt acagccggaa ccgggcgatc gcctgattga
     1741 gttcgctgga aacggtgttc aggcggtcgg cgctgctcac tacctggcgt agagcctgat
     1801 cgttctgggc gatcaggttg cgcacctgct cgaccagcgc ttcgatcttc tgttcgctgt
     1861 cgttttcggc gctgatcgcc tgcgcgatgc gcccgaccac cgtgctgacc tcctgcgaac
     1921 cagccttcat gcggtcgaag gcttcgcgcg actggcggga cagttcgacg ctctcgccaa
     1981 cctgcagcag gccggcctcc atgccctcga cggcacgctg ggtaccggac tggatgctgg
     2041 cgaccatggt cgagatttcg gatgtcgatt gtgtcgtcct ttcggccaac ttgcgcactt
     2101 cgtcggcgac cacggcgaag ccgcggccct gttcgccagc tctggcggcc tcgatggcgg
     2161 cgttgagggc gagcaggttg gtctggccgg cgatgtcgga aatgacgccg agaatcgagc
     2221 tgatcttcgc tgactgcgcg ccgagctccc ggatgtgagt cgccgtcgtt tgcaccgaac
     2281 ccgccatttc ggcagtttcc gccgccgcct tgctggccag tgcggcgccg ctgtcggacg
     2341 tggcgccgga atcgccgacg atgcgttcgg cttcgctgac cgctcgcatg atgtcgttga
     2401 ggccgctggt cgcgacatgg acgttgctga ccatttcctg ggcgtagttc aactgttgct
     2461 ggctgccggc ggcgacctgg cgcgttgcgt cgtcgaccag acgggcgcct tgctcgactt
     2521 cccgggcgtg gctggccagc gagccgacca gttgccgtag ctgatcctgc atgcgggcca
     2581 gcgcaccgag caggctggcg ccgttgctgc cgtcgagggc gatcgtccgg ctcaggtcgc
     2641 cggtagcaac ggcgctggca acctcacggg cgtaggccgg ttcgccgccc agttcgcggc
     2701 ggacgtaggc gagcgtggtc cacacgctgc cgatgccgat caggatggca atcgccgtca
     2761 gcaccagggt cagcatttcc tgattgtgcg cggcatcttc cacacgctgg cgttcttcca
     2821 gcgttttctg tttcaggatg tcgagatcgt cgagcagcac ctgcttcaac ttccgccagg
     2881 ccggcgtttc cttggcattg atcagcgcct tggcctcatc gaaacggcct tccttgagtg
     2941 ctgccagcac ttccttctgg gcgctggcct gggcttcggc cagcttttcc agtccgccga
     3001 tcgtttcggt gaaaccttcc accttcgcgg cattgacaac ggccgactgg cgcgccttgg
     3061 cgaaatcctc gcgcgccttg tcgaggttct ggtaggcctt ggggttttcc ggatcgaggg
     3121 tgatgttgcg tagtgcctgc cccatctgca agccctgggc gtacatctcg cggtaatcct
     3181 ggcgcaacgc actgggacca tcgaggaagc ggccaaaact ggccgaggtt cccagcatgc
     3241 cctgatacgc cacgccaacg gcgctgacga aagcggccat gatcagcgcc atgcccaaca
     3301 acaatcgatt gcgaaagctc atcactgtcc tcttatattt gatcactatt accattgtaa
     3361 gaggctgcgc cgggtttggg tcagcgaagg atgagcatat cttcccgatg aacgagtaca
     3421 ccggcgccgc tcaggtcggc gagcaggcgg gcggccagtt cgtcttccgc cagcgccaga
     3481 tagcgctggt tgatatcgac cgcgaacggc aggctggcca ggaaatcggc aaaacccgct
     3541 gccggcagtt gctgcctctc catcagtgcg aaggcgacga tcaccttgag ggcgtgccag
     3601 gcgagccgct cgatgtcggc gcaatagcca tcaagccggc ggaaggcggt ggcgagcgcc
     3661 cggtccacgt cggtaaacgg ggcgccgtgg ccggggatga cgatgtcgat cggcaggcgg
     3721 ccgagcattt ccagggtcgc ccgggtggcc gtcaggccgt cggcggtgcc gagcagttcc
     3781 gggaagacga cgccgaagcc gttttcccac agcgcatcgc cggaaatcag caggcgtcgt
     3841 tccgggttgt agaaggcgag cgcttccatg tcgtgccccg gtaccgccag cgcctgccag
     3901 ttcagtccgg cgaattcgat ctcgctgccg gcctcgatca gcgcgtcgtg gcggaagggg
     3961 gcagcctgct ggccgagcgg cgagagcagc agggcatcct cgtcccattc ggcgatgctt
     4021 gcctggaggc cggcggggac cccgatgctg cagccgaaag ccgcctgc
//
LOCUS       DAOSDX010000148         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7776_length_4066_cov_3.341311, whole genome shotgun sequence.
ACCESSION   DAOSDX010000148 DAOSDX010000000
VERSION     DAOSDX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7776_length_4066_cov_3.341311"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLN02_04355"
     CDS             complement(<1..540)
                     /locus_tag="PLN02_04355"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOI51585.1"
                     /translation="MPQYRSRTSTHGRNMAGARALWRATGMKDGDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAREIEAAGGVAKEFNTIAIDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVSWKDQTI
                     KVDLIDAMIEAADPKNSDKE"
     gene            630..1421
                     /gene="lgt"
                     /locus_tag="PLN02_04360"
     CDS             630..1421
                     /gene="lgt"
                     /locus_tag="PLN02_04360"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOI51586.1"
                     /translation="MLLHPQFDPVAIYLGPLAIRWYGLMYLLAFLQFIALGRLRIRQK
                     LAPVSAEQLDDLLFYGVLGVVLGGRLGQVLFYEPGYYLANPLEILAVWKGGMSFHGGF
                     LGVLVAMTLWAKKAGLRWLTVTDFIAPLVPLGLAAGRVGNFINGELWGRLADPSLPWA
                     MVFPQSGSLEPRHPSQLYHVGLEGLALFAILWLWTAKPRPTGAASGVFLIGYGAFRFI
                     TEYFREPDHGIFGQSYTVSMGQWLSLPMVLAGVALVVFAYRRKSL"
     gene            1463..1936
                     /locus_tag="PLN02_04365"
     CDS             1463..1936
                     /locus_tag="PLN02_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI51587.1"
                     /translation="MIRPFKVLGIQQIAIGGPSKEKLKTLWVDMLGLEVTGTFMSERE
                     NVDEDICAMGKGPFKVEVDLMQPLDPEKKPAVHTTPLNHVGLWIDDLPKAVEWLSANG
                     VRFAPGGIRKGAAGFDICFLHPKGNDEFPIGGEGVLVELVQAPPEVVEAFAKLAG"
     gene            1959..2720
                     /locus_tag="PLN02_04370"
     CDS             1959..2720
                     /locus_tag="PLN02_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51588.1"
                     /translation="MAQFPDDDIEKTRSALAPTMDAMASILPWVGKTQPARYPPELNK
                     RWQTACRALADDWSKHGRSDPETIRPRVFALLAVAIETGEADCLRFGETLASVADHLE
                     HKAPGNRLSAALSAATEALLDEGGLENPHFGERLRHFTGRLEAALRPSSKPGERSDVL
                     DRLFVQDAEERVARMHEALEVLPIDVYAIELEASELIQHAEQIEMWGIYHLTRQVLNF
                     ALQLGDASEAVQDQAARDIEQQLRLIEQALRNIDY"
     gene            complement(2773..3651)
                     /gene="htpX"
                     /locus_tag="PLN02_04375"
     CDS             complement(2773..3651)
                     /gene="htpX"
                     /locus_tag="PLN02_04375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOI51589.1"
                     /translation="MKRVVLFLLTNLAVMLVLSVVTSLLGVNRFLTANGLNLGMLLAF
                     AAVIGFGGSFISLLMSKPMAKWSTGARVIEHPSSSTEVWLVDTVARLAKRANLPMPEV
                     AIYDGEPNAFATGASKNSSLVAVSTGLLQSMTREEAEAVLAHEVAHIANGDMVTLTLI
                     QGVVNTFVIFISRVVGYLVDSFLRRNDSESSGPGIGYMVTSMVCEIVFGVLASIVVAW
                     FSRQREFRADAGAAGLMGSPVPMQNALRRLGNLHTEPLPQNMAASGIAGGKGGFMALF
                     ATHPPLEERIAALGGR"
     gene            3754..>4066
                     /locus_tag="PLN02_04380"
     CDS             3754..>4066
                     /locus_tag="PLN02_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI51590.1"
                     /translation="MLNYKHLHYFWVVAKEGSITRAAERLGVAVQTISGQLGLLERQL
                     GKTLFNNQGRGLVLSDAGRKALAYADQIFLLGEQLEEALQHDGDDAILRLRVGISDGI
                     PK"
BASE COUNT          772 a         1328 c         1250 g          716 t
ORIGIN      
        1 ctccttgtcg ctgttcttcg ggtcggcggc ctcgatcatc gcgtcgatca gatcgacctt
       61 gatcgtctgg tccttccagc tgaccttgcc agcctccatc gggccgccgg agacaaagac
      121 gaccgggatg ttcagacgca tcgccgccat cagcattccg ggggtgatct tgtcgcagtt
      181 cgagatgcag accatcgcat cggcgcaatg ggcattgacc atgtattcga ccgaatcggc
      241 gatcaggtcg cgcgacggca gcgagtagag catgccgcca tggcccatgg cgatgccgtc
      301 gtcgatggcg atggtgttga attccttggc gacaccgccg gccgcctcga tctcgcgggc
      361 gaccaattgg cccatgtcct tgagatggac gtggccggga acgaactggg taaagctgtt
      421 gacgacggcg atgatcggct tgccgaaatc gccatccttc atgccggtcg cgcgccacag
      481 ggcgcgggcg ccggccatgt tgcggccatg ggtggaagtg cgggaacggt actggggcat
      541 gctcactctc cgtttagtat ctggcgccgg cacgtagctg tcacggacgc gatctataat
      601 cggccaattc tagccgaatc gattcgacca tgctgctcca tcctcagttc gatcccgtcg
      661 ccatttacct cggtccgctc gccatccgct ggtacggcct gatgtacctg ctcgccttcc
      721 tgcagttcat tgcgctcggc cgtctgcgca tccgccagaa gctggcgccg gtcagcgccg
      781 aacaactcga cgacctgctc ttctacggcg tgctcggcgt cgtgctcggc ggccgcctcg
      841 gccaggtgct gttctacgag cccggctact acctggccaa tccgctcgag atcctcgccg
      901 tctggaaagg cggcatgagt ttccacggcg gcttcctcgg cgtcctcgtg gcgatgacgc
      961 tatgggcgaa aaaggccggc ctgcgctggc tgaccgtgac cgacttcatc gcgccgctgg
     1021 ttccgctcgg cctcgccgcc ggtcgcgtcg gcaacttcat caacggcgaa ctctggggcc
     1081 gcctggccga tccgtccctg ccctgggcga tggtctttcc gcaatcgggc agcctcgaac
     1141 cgcgccaccc gtcgcagctc taccacgtcg gcctcgaagg cctggcgctg tttgccatcc
     1201 tctggctatg gaccgcgaaa ccgcgcccga ccggtgccgc ctcgggcgtc ttcctgatcg
     1261 gctacggcgc cttccgcttc atcaccgagt atttccgcga acccgaccac ggcatcttcg
     1321 gccagtcgta cacggtgagc atgggacagt ggctgtcgct gccgatggtg ctggccggcg
     1381 tcgcgctggt ggtctttgcc taccgccgga aatccctata attatggatt actcaaacca
     1441 cagcccgaca gggagataac gcatgattcg tccgttcaag gttctcggca tccagcaaat
     1501 cgccatcggc ggcccgtcca aggaaaaact caagaccctg tgggtcgaca tgcttggcct
     1561 cgaggtcacc ggcaccttca tgtcggagcg cgaaaacgtc gacgaagaca tctgcgcgat
     1621 gggcaagggc ccgttcaagg tcgaggtcga cctgatgcag ccgctcgatc cggaaaagaa
     1681 accggccgtg cacacgacgc cgctgaacca cgtcggcctg tggatcgacg acctgccgaa
     1741 ggccgtcgag tggctgtccg ccaacggcgt ccgcttcgcc ccggggggca tccgcaaggg
     1801 cgctgccggc ttcgacatct gtttcctgca cccgaagggc aacgacgaat tcccgatcgg
     1861 cggcgaaggc gtgctcgtcg aactggtgca ggcgccgccc gaagtggtcg aggctttcgc
     1921 taaactagcg gggtaatcac cacacaggag cgaggacgat ggcccaattt cccgacgacg
     1981 acatcgagaa aacccgttcc gccctcgctc cgacgatgga cgccatggcg tccatcctgc
     2041 cctgggtcgg caagacccag ccggcgcgct acccacctga actcaacaag cgctggcaaa
     2101 ccgcctgccg ggcattggcc gatgactggt cgaagcacgg gcgcagcgat ccggaaacga
     2161 tccgcccccg ggtcttcgcg ctgcttgccg tcgccatcga aaccggcgaa gccgactgcc
     2221 tgcgctttgg cgaaaccctg gccagcgtag ccgatcacct cgagcacaag gcgccgggca
     2281 accgtctgag cgccgccctg agcgcggcta ccgaagccct gctcgacgaa ggcggcctgg
     2341 aaaatccgca tttcggcgaa cgcctgcgcc atttcaccgg ccgcctggaa gccgccttgc
     2401 gcccgtcgag caagcccggc gaacgttccg acgtactaga ccgcctcttc gtgcaggacg
     2461 ccgaagagcg cgtcgcccgc atgcacgagg cgctcgaagt gctgccaatc gacgtctacg
     2521 cgatcgaact ggaagccagc gaactgatcc agcacgccga gcagatcgag atgtggggga
     2581 tctatcacct gacccggcag gtgctcaatt tcgcgctgca actcggtgac gcaagcgaag
     2641 cggtgcagga ccaggccgcc cgggatatcg agcagcagtt gcggctgatc gagcaggctt
     2701 tgcgcaacat cgactactga agccccaccc gccaaaaaaa gggagaccgc ggtctccctt
     2761 tttgcaggtc gctcagcgtc cgccgagggc ggcgatgcgc tcctcaagcg gcgggtgggt
     2821 ggcgaaaagc gccatgaatc cgcccttgcc gccggcgatg ccggaagccg ccatgttctg
     2881 cggcaaaggt tcggtatgca ggttacccag acgacgcagc gcgttctgca tcggcaccgg
     2941 cgagcccatc aacccggcag caccggcgtc ggcgcggaat tcacgctgcc gagagaacca
     3001 ggcgacaacg atgctggcca gaacgccgaa gacgatctcg cagaccatgc tggtgaccat
     3061 gtagccgatg cccggtccgc tgctctcgga atcgttgcgg cgcaggaagg aatcgaccag
     3121 atagccgacg acacgcgaaa tgaagatgac gaaggtgttc acgacgccct gaatcagcgt
     3181 cagcgtcacc atgtcgccgt tggcaatgtg cgccacctca tgcgccagta ccgcttcggc
     3241 ttcctcgcgg gtcatgctct gcagcaggcc ggttgacacc gcgaccagcg aactgttctt
     3301 gctggcgcca gtggcgaaag cattgggttc gccatcgtag atcgccactt ccggcattgg
     3361 caggttggcg cgtttggcca ggcgggcgac ggtatcgacc agccagactt cggtggatga
     3421 cgacgggtgc tcgatgacgc gagcgccggt gctccacttg gccatcggct tggacatcag
     3481 cagcgagatg aaggagccgc cgaagccgat caccgcggcg aaagccagca gcatgccgag
     3541 attgagacca ttggcggtga ggaagcgatt gacgccgagc agactggtca ccacgctgag
     3601 taccagcatg accgccaggt tggtcagcag gaaaagtacg acgcgtttca tgttgaactc
     3661 cgggtgtgat gaaaacggcg ccatgttaac acgcgttgga ataacgaaaa aaacgtagca
     3721 aaattgaggc gaacttcgtt tttttcgaaa gccatgctca actacaaaca cctccactac
     3781 ttttgggtcg ttgccaagga aggtagcatc acccgcgctg ccgagcgcct cggcgtcgcc
     3841 gtgcagacga tcagcggcca actcggcctg ctcgagcgcc aactgggcaa gacgctgttc
     3901 aacaatcagg gacgcggtct ggtcctcagc gacgccgggc gtaaggcgct ggcctacgct
     3961 gaccagattt tcctgctcgg cgagcaactg gaagaagcct tgcagcacga tggcgacgac
     4021 gccatcctgc ggctgcgcgt cggcatctcc gacggcatcc cgaagc
//
LOCUS       DAOSDX010000149         4050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7820_length_4050_cov_3.557196, whole genome shotgun sequence.
ACCESSION   DAOSDX010000149 DAOSDX010000000
VERSION     DAOSDX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7820_length_4050_cov_3.557196"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..671)
                     /locus_tag="PLN02_04385"
                     /pseudo
     CDS             complement(1..671)
                     /locus_tag="PLN02_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289849.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            1055..1930
                     /gene="dapA"
                     /locus_tag="PLN02_04390"
     CDS             1055..1930
                     /gene="dapA"
                     /locus_tag="PLN02_04390"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOI51591.1"
                     /translation="METNFEGIWVPIITPFSDGQVDHPALAKLAGHLAAGGVAGLVVG
                     ATTGEGALLLPGEQEAIFATLRQAVPQLPLLLGVSHASTQLAAEQARRLSALAPEGFL
                     VTPPVYVRPSQEGVRRHFEAIVEAADRPLLIYNIPYRCGVTVEVETIAALARDPRIVG
                     IKECGGSIERLARLIQETPLRVFSGEDHLNFPALCLGGHGTIAASAHLRPELHVRMFE
                     CCRQGRLAEARDLAAALQALVHDLFAEPNPAPLKSILASQGWCANELRLPFVPASADL
                     AERLLQHWEQLPQDD"
     gene            complement(2146..3078)
                     /gene="cysK"
                     /locus_tag="PLN02_04395"
     CDS             complement(2146..3078)
                     /gene="cysK"
                     /locus_tag="PLN02_04395"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOI51592.1"
                     /translation="MKIANNVTELVGNTPLVQLNRVTEGAGARVVAKLEFFNPGHSVK
                     DRIAVAMLDAAQAAGKIGPDTVVLEPTSGNTGIGLAMVCAARGIKCAFTMPETMSRER
                     KLLLKAYGAELILTPGPEGMGGAIAKAKALAEADAKYFIPQQFENPANPAIHRATTAE
                     EIWRDTDGQVDIFVSGVGTGGTVTGVGEVLKARKPGVKIVAVEPDASPVLSGGAKGPH
                     PIQGIGAGFVPGVLNTGVYDEVIRVKNDDAFTTARQMATEEGLLVGISSGAATWAALE
                     LAKRPENAGKLIVVIIPSFGERYLSTPLYQHLEV"
     gene            complement(3173..>4050)
                     /gene="ilvA"
                     /locus_tag="PLN02_04400"
     CDS             complement(3173..>4050)
                     /gene="ilvA"
                     /locus_tag="PLN02_04400"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HOI51593.1"
                     /translation="GHRVRLPQVGLFADGTAVKFVGEETFRLCREFLDDIVLVDTDAI
                     CAAIKDVFEDTRSILEPSGALAVAGAKEYARQHKLKDKNLVAVTSGANMNFDRLRFVA
                     ERAEFGEQREAVFAVTLPEKPGAYKKFLALIGKRNVTEFNYRYHSASEAHVFVGVQVS
                     DRKESLKLVDSLQKHGYPTLDLSDDEMAKNHIRHMVGGHAPQVCENGMRELLYRFEFP
                     ERPGALMNFLSQMSAGWNISLFHYRNHGADYGRVLVGMQVPPAEMAQFREFLKNLGYA
                     HWDESDNPIYKLFLG"
BASE COUNT          678 a         1283 c         1329 g          760 t
ORIGIN      
        1 cgccatcagc gtgacgccgc ccggcgcagc atcacgctga tggcgaaggg aatgtgtttt
       61 ttgtcttcgt cgagcaccag gccgcagtgg atttcgtcgg aacagacgat cagctcacgc
      121 cgccgcgcct gttcggcgag atcgagcaat tcctcgcgcg accagcagcg gccgaccggg
      181 ttgtgcgggt ggcagagcag aaacagcgaa tttcctgcgg gcgacgcgtt ttccagtcca
      241 ttttttggcc attgccagcg gccgtcgcgc agttccagct cgatccgccg cagcgtccgc
      301 ccggcgtggc gcggcgccga caggaaaggc ggataaaccg gcgtcgcgct caacacctcg
      361 ccctcgaccg tgcggcaggc aacattcagc ccggtaacca gccccggcag ccagacgatc
      421 cactcggccg cgatgcgcca ggcgtactcg ccagccagat gatcctgcac cgcttccgtc
      481 agcgacggcc aaggcccgcc gtaaccgaag acgccatgct cgatgcgccg gtgcagcgcg
      541 gccagcaccg ccggcggcgc ggcaaaatcc atgtccgcca cccacagcgg caaaatatcc
      601 cgcccggcgt acttgcgcca tttgatcgaa tcggaggtgg aacggtcgat gggggtgtcg
      661 aagggggaca tggtgcggag gaaaagggaa taacttgttc ggacgcagca tagcggaatc
      721 cggggagggt tgccgtgcaa accctgcaag acagtgaccc tgtcggctat tcccggaatc
      781 gcagcgggtg cgctatgatc ggccgctcag accacctttc cttactggct atgtccttgc
      841 atcgacccgc aaccttactg tccggcgcgc ctgcgtcggc gtcggtgtgc gcgtctggtc
      901 tgaaagcgaa aaaacgactt ctccgctgac ggggatgtcg tgtcccgtcg ggcggattgc
      961 cggacgggac tgaccggggc ttgctgccct gatcctgcct cagccggcca attgccggcg
     1021 gaatgatctt catcaatcca atcatgaggc aaacatggaa acaaacttcg aaggcatctg
     1081 ggttcccatc atcaccccct tttccgacgg gcaggtcgat catcccgcac tggcgaagct
     1141 cgccggccac ctggcggccg gtggcgtagc cggtctggtg gtcggcgcca ctacgggcga
     1201 aggcgccttg ctcctgcccg gcgaacaaga agcgatcttc gccacactcc ggcaggcggt
     1261 gccgcaactc ccgctgctgc tgggcgtctc ccatgcctcg acccagcttg cggccgagca
     1321 ggctcggcgc ctctcggcgc tggcgccgga aggtttcctg gtgacgccgc cggtctatgt
     1381 gcgcccgtcg caggaagggg tgcgtcggca tttcgaggcg atcgtcgagg cggcggaccg
     1441 gccgttgctg atctacaaca ttccctaccg ctgcggcgtc actgttgaag tcgagaccat
     1501 cgccgcgctg gcgcgggacc cgcgtatcgt cggcatcaag gagtgcggcg gcagcatcga
     1561 acggcttgcc cgcctgatcc aggaaacgcc gttacgtgtg ttctccggcg aggaccacct
     1621 gaatttcccg gcgctttgcc tgggcgggca cggcaccatt gccgcatcgg cacacctgcg
     1681 cccggagttg cacgtccgca tgttcgaatg ctgccgccag ggccgcctcg ccgaggcgcg
     1741 ggatctggct gctgcgctgc aggcgctcgt tcatgacctg tttgccgaac ccaacccggc
     1801 accgctcaag tcaatcctgg ctagccaggg ctggtgcgcc aacgaattgc gcctgccttt
     1861 cgtaccggcc agcgccgacc tcgcagaacg actgctgcag cactgggaac aactgccgca
     1921 ggatgattga cacaccaagt gctgcaattc cggtgcgcgc tacggccggg gttgcagttg
     1981 cgtccgtggg gaggaatcca ttttctggtt ggatacggga ttcttcttcc ggattacgca
     2041 aaaaaaaacc gccccgtcgc gcgccggcga gggagcgatc gttttgcggc cgaaaccgcc
     2101 ggtttttcag gctcaggcgc caaccgacgc cctcaccctt gaacctcaaa cctccagatg
     2161 ctggtacagg ggcgtcgaca aatagcgctc accgaaggaa gggatgatga cgacgatcag
     2221 cttgccggcg ttttccgggc gcttggccag ttccagtgcg gcccaggtgg cggcgccgga
     2281 ggagatgccg acgagcaggc cttcttcggt ggccatctgg cgggcggtgg tgaaggcgtc
     2341 gtcgttcttg acgcggatca cctcgtcgta gacgccggtg ttgagcacgc cggggacgaa
     2401 accggcgccg atgccctgga tcgggtgcgg gcctttcgcg ccgccggaca gcaccggcga
     2461 agcgtcgggt tcgacggcga cgatcttgac acccggtttc ctggccttga gcacttcgcc
     2521 gacgccggtg acggtgccgc cggtgccgac gccggagacg aagatatcga cctggccgtc
     2581 ggtgtcacgc cagatttcct cggcggtggt tgcgcggtgg atggccgggt tggccgggtt
     2641 ctcgaactgc tgcgggatga agtacttcgc gtcggcttcg gccagcgcct tggccttggc
     2701 gatggcgccg cccatgcctt ccggtcccgg cgtcaggatc agttcggcgc cgtaagcctt
     2761 gagcagcagc ttgcgttcgc ggctcatggt ttccggcatg gtgaaggcgc atttgatgcc
     2821 gcgcgcggcg cagaccatgg ccaggccgat gccggtgttg ccggaggtcg gttccagcac
     2881 cacggtgtcc gggccgatct tgccggcggc ctgtgccgca tcgagcatgg cgacggcgat
     2941 gcggtccttg acgctgtggc cggggttgaa gaattccagc ttggcgacga cgcgggcgcc
     3001 ggcgccttcg gtgacgcggt tgagctggac cagcggcgtg ttgccgacca gttcggtgac
     3061 gttgttggcg attttcatgg gggttctccg gaagggttga tggctagcag tctagaactt
     3121 gggctacctt accggaaaga atgaaatcgc aaattcttat cacctttttg cattagccga
     3181 ggaagagctt gtagatcggg ttgtcgcttt cgtcccagtg cgcgtagccg aggttcttca
     3241 ggaactcgcg gaactgcgcc atctcggccg gcggcacctg catgccgacg aggacgcgac
     3301 cgtaatcggc gccatggttg cgatagtgga acaggctgat gttccagccg gcgctcatct
     3361 ggctcaggaa gttcatcaat gcgcccggcc gctccgggaa ttcaaagcgg tagagcaact
     3421 cgcgcatgcc attttcgcaa acctgcggcg catggccacc gaccatgtgg cggatgtggt
     3481 tcttcgccat ctcgtcgtcg ctcaggtcga gcgtcggata gccgtgcttc tgcaggctgt
     3541 cgaccagctt cagcgattcc ttgcggtcgc tgacctggac gccgacgaag acgtgcgctt
     3601 cgctggccga gtgataacgg tagttgaact cggtgacatt gcgcttgccg atcagcgcca
     3661 ggaatttctt gtaggcaccg ggcttttccg gcagcgtcac ggcgaacacc gcctcgcgct
     3721 gctcgccgaa ttcggcacgc tcggcgacga agcgcaggcg gtcgaaattc atgttggcgc
     3781 cggaggtcac ggccaccagg ttcttgtcct tcagcttgtg ctggcgcgcg tattccttgg
     3841 cgccggcgac ggctagcgcg ccggacggtt cgagaatcga acgggtgtcc tcgaagacat
     3901 ccttgattgc cgcgcagatg gcgtcggtat cgaccagcac gatatcgtcg agaaactccc
     3961 ggcacaggcg gaaggtttcc tcgccgacga acttcaccgc cgtgccgtcg gcgaacagcc
     4021 cgacctgcgg caggcgcacg cggtggccct
//
LOCUS       DAOSDX010000150         4047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7827_length_4047_cov_3.364479, whole genome shotgun sequence.
ACCESSION   DAOSDX010000150 DAOSDX010000000
VERSION     DAOSDX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7827_length_4047_cov_3.364479"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1495
                     /gene="mnmE"
                     /locus_tag="PLN02_04405"
     CDS             140..1495
                     /gene="mnmE"
                     /locus_tag="PLN02_04405"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOI51594.1"
                     /translation="MAVVNNDTIAAIATAPGRGGVGVIRISGSNLLPFAFALTGKTPK
                     ARYATLADFRAGDGATIDSGLLLYFPAPHSFTGEDVLELQGHGGPVVMQMLLGRCLDL
                     GARLAEPGEFSRRAFLNGKMDLAQAEAVADLIDAATASAARSAVRSLQGEFSRAIGEL
                     NAELINLRMLVEATLDFPEEEIDFLKAADAFGRLERLQRKLAEIFDRAGQGKLLQSGL
                     HVVLVGQPNVGKSSLLNRLAGDELAIVTPIAGTTRDALRSTIQIAGIPLHIIDTAGLR
                     DTDDEVERIGIARTWQEIERADVVLLLVDARAGIADADRAILARMPERLQRITVHNKI
                     DLLAQAPERHPESDGIAISLSAKANLGIDLLRQELLRIAGWHQTEDVFIARERHLRAL
                     ASAQDHVAAARAIVESGFPALELFAEELRLAQQALGEITGEFTADDLLGVIFSRFCIG
                     K"
     gene            complement(1736..2377)
                     /locus_tag="PLN02_04410"
     CDS             complement(1736..2377)
                     /locus_tag="PLN02_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51595.1"
                     /translation="MKRAALILAVSLLAACASHTPPPDWLLRADAARDAHARFWLEGR
                     DKLADGQLALARAAVRQTGDASAMARIELHACAVRMAALGAADCPAFAELADDAGRRE
                     QVYGAYLAGHLDNADIGLLPEAQALAWRNPAALKELADPLSRLLAAAVLFRSNRLPPD
                     GIALAIDTASAQGWRRPLLAWLTLERERRQRNGDAEGAAAIERRLARIVGERR"
     gene            complement(2374..3222)
                     /locus_tag="PLN02_04415"
     CDS             complement(2374..3222)
                     /locus_tag="PLN02_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOI51596.1"
                     /translation="MSTPPPVSPIAHPEAKAIGLLLLTLVLVAAFVVYVMNARGVFED
                     NQHLLLVAENSEGVAVGMDLTFSGFPIGRVTRIELGDDGKAHIHLDIPRRDARWLRSS
                     SVFTLERGVVGGARLRAYSGLLDDPALEDGAKREVLIGDASSGVPELVSTMHRLVANV
                     ERLTAEDAALSVTLDNLRQFTAALNGRHGALGALLGNEKDVGRVIAALEQSRQLLTDA
                     RASLQKVDAALVDVKAITGNTRAATEDLDALRSEVDLNLRKVSGLIDDLNRKWPFARE
                     RKLELP"
     gene            complement(3227..3970)
                     /locus_tag="PLN02_04420"
     CDS             complement(3227..3970)
                     /locus_tag="PLN02_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51597.1"
                     /translation="MPTPKLLRQTAVWIHGQYRYLRFAALLFIAACSPRIHDRATRNH
                     TARVLCAAAWQPLAGFLLASLLISAVLTRIVAVTADSYGLTHLALEAILRVFVVEVLP
                     LASTLFVAMRAVPMAMQCLCAHAGHHDAVLRAALPYAIGNAAAVVILAVIGGFVTLLV
                     AYPVVHGFSPWALPAYGRLIGQVFDPVLAIGFAAKIALFGIAVGIAPTTVALDPRQRD
                     PGLREMRVMVRLLLVLVLIEASFLMLRGF"
BASE COUNT          734 a         1279 c         1359 g          675 t
ORIGIN      
        1 gtcatgttcc tcttcttccc gtccgggctg gtgctgtact gggtggtgaa caacattctg
       61 tcgattgccc agcagtggca gatcacccgc atgatggaaa agggcggcaa ggctgccaac
      121 gacgcgaaag cctgagggtg tggcagttgt aaacaatgac accatcgccg ccattgccac
      181 ggctccaggc cgtggcgggg tgggcgtcat ccgcatttcg ggcagcaact tgctgccctt
      241 tgcttttgca ctgaccggca agacgcccaa ggcgcgctac gcgacgctgg ccgattttcg
      301 ggccggcgat ggcgcgacca tcgacagcgg cctgctgctt tactttcctg ccccgcattc
      361 cttcaccggc gaggatgttc tcgaactaca gggccacggc ggcccggtcg tcatgcagat
      421 gctgctcggc cgttgcctcg acctcggcgc ccggctcgcc gaaccgggcg aattcagccg
      481 gcgcgccttc ctcaacggca agatggacct tgcccaggcc gaagccgtcg ctgacctgat
      541 cgacgccgcc acggccagcg ccgcgcgttc cgcggtacgt tcgctacagg gcgaattctc
      601 gcgggcgatc ggcgaactga atgcggaact gatcaacctg cgcatgctgg tcgaagcgac
      661 cctcgatttt ccggaagagg agatcgactt cctcaaggcc gccgacgcct tcggccgcct
      721 cgaacgcctg cagcgcaagc tggcggaaat ctttgaccgc gccggccagg gcaagctgct
      781 gcagagcggc ctgcacgtcg tcctggttgg tcagccgaat gtcggcaagt cttcgctgct
      841 caaccgcctg gccggcgacg agctggccat cgtcacaccg attgccggta ccacgcgcga
      901 cgcgctgcgc tcgaccatcc agattgcagg catcccgctg catatcatcg ataccgccgg
      961 cctgcgcgat accgacgacg aagtcgagag aatcggcatt gcccgcacct ggcaggagat
     1021 tgaacgcgcc gacgtggtgc tgttgctggt cgacgcccgc gccggcatcg ccgatgccga
     1081 ccgcgccatc ctggcccgga tgccggaacg cctgcagcgg atcaccgtgc acaacaagat
     1141 cgacctgctc gcgcaggcgc cggaacgcca ccctgaaagc gacggcattg ccatctcgct
     1201 atccgccaag gccaatctcg gcatcgacct gttgcgccag gagctgctgc gcatcgccgg
     1261 ctggcaccag acggaggatg tcttcatcgc ccgcgaacgc catctgcgag cgctcgccag
     1321 cgcccaggat cacgtcgctg cagcacgcgc catcgtcgaa agcggttttc cggcgctcga
     1381 actgtttgcc gaagagttgc gcctggcgca gcaggcactc ggcgaaatca ctggtgaatt
     1441 taccgccgac gacctgctcg gcgtgatttt cagccgtttc tgcatcggca agtagggctg
     1501 tcgataagac tgtgcacaac ccctgtgcgg gatgtgcacg attggcggaa ttttccctgg
     1561 cgccgattct gtccccattt catccacaag ctgcgcaggt acttgcccgc aaccgaaaac
     1621 ttgtctcaat agactgattt atttctgtaa acataagtta tccacagagt tgtcaacgct
     1681 gtagttaaca tagtcttttt atttaaggta tttattggta aaccgaaagc cactgtcagc
     1741 gccgctcgcc gacgatgcgt gcgaggcgtc tttcgatcgc ggcggctccc tcggcatcgc
     1801 cgttccgttg acgccgttca cgttccagcg tcagccaggc cagcaaggga cgacgccagc
     1861 cctgagcgct ggccgtatca atggccagtg caatgccatc cggtggcaga cgattgctcc
     1921 tgaacagcac ggcggcggcc agcagccgtg acaaggggtc ggcaagctcc ttcagggcgg
     1981 caggattgcg ccaggcgagc gcctgcgctt ccggtagcag gccgatatcg gcgttgtcca
     2041 gatggccggc aaggtaagcg ccatagactt gttcgcggcg accggcatcg tcggccagtt
     2101 cggcaaaagc cggacaatcg gcagcgccca gtgccgccat gcggacggcg caggcgtgca
     2161 attcgatgcg cgccatcgcg ctggcgtcac cggtctgccg cacggcagcg cgggccaggg
     2221 cgagttggcc gtcagccagc ttgtcacggc cttccagcca gaagcgggcg tgcgcgtcgc
     2281 gcgccgcgtc ggcccggagc agccagtccg gcggcggggt atggctggcg caggcggcga
     2341 gcagggaaac ggcgaggatc agggcagcgc gtttcatggc agctccagct tgcgttcgcg
     2401 ggcaaacggc cacttgcggt tgagatcgtc gatcagcccg gaaaccttgc gcaggttgag
     2461 gtcgacctcg ctgcgcaggg cgtcgaggtc ttcggtggcg gcgcgggtat tgccggtgat
     2521 tgccttgacg tcgaccagtg ccgcatcgac tttctgcaga ctggcgcggg cgtcggtcag
     2581 cagttgccgg ctctgctcca gcgcggcaat gacgcgaccg acatccttct cgttgccgag
     2641 cagcgcgccg agcgcgccat gccggccgtt gagggcggcg gtgaactgac gcaggttgtc
     2701 gagggtcacg gacagcgcgg catcttccgc agtcagccgt tcgacgttgg cgaccagacg
     2761 atgcatcgtg ctgaccagtt cggggacgcc cgatgaggcg tcgccgatca ggacttcgcg
     2821 tttcgccccg tcttccagcg ccggatcgtc aaggaggccg ctgtaggcgc gcaggcgggc
     2881 gccgccgact accccccgtt ccagggtgaa gacggagctg ctgcgcagcc agcgtgcgtc
     2941 gcggcggggg atatcgaggt ggatgtgcgc cttgccgtcg tcgccgagct cgatgcgggt
     3001 cacccgaccg atcgggaagc cggagaaagt cagatccatg ccgaccgcga cgccttccga
     3061 attctcggcg accagcagca gatgctggtt gtcttcgaag acgccgcgcg cgttcatgac
     3121 atagacgacg aatgccgcca ccaggacgag agtgagcagg agcaggccga ttgccttggc
     3181 ttcgggatgg gcgatcgggg aaacgggtgg cggtgtcgac atgagttcaa aatcctctca
     3241 acatcaggaa gctggcttcg atcaggacca ggaccagcag caggcggacc atgacgcgca
     3301 tttcgcgcag tccggggtcg cgctggcgcg ggtcgagcgc gacggtggtc ggggcaatgc
     3361 cgacggcgat gccgaagagt gcgatcttgg cagcaaagcc gatggccagg acgggatcga
     3421 agacctggcc gatcagccgg ccataggccg gcaaggccca gggactgaag ccatggacga
     3481 ccgggtaggc gaccagcagc gtgacgaagc cgccgatcac ggcaaggatg acgacggcag
     3541 cagcattgcc gatggcgtac ggcaaggcgg cgcgcaacac cgcatcgtgg tgaccagcgt
     3601 gagcacacag gcattgcatc gccatcggca cggcgcgcat ggcgacgaac aaggtggagg
     3661 ccagcggcag aacctcgacg acgaagacgc gcaggatggc ttccagcgcc aggtgggtca
     3721 gaccgtagct gtcggcggtg acggcgacga tgcgggtcag tacggcgctg atcagcaggc
     3781 tggccagcag gaagccggcc agtggttgcc aggcggcggc gcagagcacg cgggcggtgt
     3841 ggttgcgggt ggcgcggtca tgaatgcgcg gcgagcaggc ggcgatgaac agcaaggccg
     3901 caaagcgcag ataccggtac tgtccgtgaa tccagacggc agtctggcgc aggagtttcg
     3961 gcgttggcac ggacccggcc aggatcaggc gcgctcggcc tccagccgtt tgcggcgcag
     4021 gacctgcgcc gcaaacggct ggaggcc
//
LOCUS       DAOSDX010000151         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7834_length_4045_cov_2.840852, whole genome shotgun sequence.
ACCESSION   DAOSDX010000151 DAOSDX010000000
VERSION     DAOSDX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7834_length_4045_cov_2.840852"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="PLN02_04425"
     CDS             <1..121
                     /locus_tag="PLN02_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOI51598.1"
                     /translation="GVRYDCGSKLGYLKATVAFGRRHPEVGADFAAYLDGEPG"
     gene            118..807
                     /locus_tag="PLN02_04430"
     CDS             118..807
                     /locus_tag="PLN02_04430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI51599.1"
                     /translation="MSGAKLDSFLDYWESEGETLARHGDYDWMAALTPGRRVLEIGCG
                     PGFSTLALVQRGLDVLVVDAQPACLALARQRVGALPLAVLEAEVTALSAEQRAAILAF
                     APESVVCWLMGAPAEVTGASAGDGGKAVVAYRERVHRAVAELAADLPSVSALHLVDRT
                     AIPWQAKDIGRATLLGYHRQKTLAGLPFQAQREDALYRKLEAAAPSFKPHAALKGVVP
                     VLASLLAYRSS"
     gene            809..1579
                     /locus_tag="PLN02_04435"
     CDS             809..1579
                     /locus_tag="PLN02_04435"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HOI51600.1"
                     /translation="MLAIIGGSGLTTLSNLDVSHREVVRTPYGEASGALVFGQICGRP
                     AVFLPRHGYGHTIPPHMVNYRANLWALHHHQVSGIISVASVGSIRPDLQPGDIVLPDQ
                     IIDYTWGRKSTFFDGVGSPVTHIDFTEPYDAALARRIAEAAAALGIGVAVGAVYAATQ
                     GPRLESAAEINRLERDGADVVGMTGMPEAALARELGVPYAAINVVANHAAGRGSSANG
                     IRFDSLEHVLQEAMGRVKAIIERVVGCEAACPPLGMSV"
     gene            complement(1592..>4045)
                     /locus_tag="PLN02_04440"
     CDS             complement(1592..>4045)
                     /locus_tag="PLN02_04440"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:NF002837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOI51601.1"
                     /translation="HSRLVDVLLQDLWQAAGISPQVCLIAVGGYGRGELFPHSDVDLL
                     ILLPQAPSANTAEKLERLVGCFWDIGLEIGHSVRTIPECLDEATRDITVQTALLEARL
                     LTGSEKLFATFRKRLFGNLDPLGFFEAKRLEQQDRYLRHNETPYSLEPNLKESPGGLR
                     DQQVIFWIAKAAGFGDTWDELGKGGVMSQEGVAFGKQFEDFLRHLRIRLHFIARRRED
                     RLLFDYQSALAARYGFQDSESKRASEQLMQAYYRNAKGLMLLNTIVLQNMGAALAPEN
                     LQRPQAYDANFQIVGNLLDICDENLFERQPGLIFDAFRIMQENSELQGMTASTLRALW
                     LARERIDDGFRADPANRAMFLGLLQAERGVIHEFRRMNQLDILGAYLPNFGRIVGQMQ
                     HDLFHVYTVDQHILQVLRNLRRFTMSEFAHEYPLCSRTINEFEEPWLLYIAALFHDIA
                     KGRGGDHSEKGTVDAREFCDNHGLTETDTELVVWLVRHHLVMSQIAQKEDLGDPEVIG
                     RFAALVGDVRHLQALYLLTHADVRGTSPKVWNQWKGKLLADLYQQTLHHLAHDEPPVS
                     HGIIADRQAEAMRLLRFFALSDTVHERLWKQLDTVYFLRHSAEEIAWHTRSLHYRIHN
                     NQPVVRARLWQEGVGLQVMVYTRDQRDLFARLVGFFARAGYSIVDAKIHTTEHGYALD
                     SFILLDVERREHNREMLSYIEHELEDRLTRELPPEIPSAGRLSRQVRHFPIKPEVSIS
                     GDEKGKHFILSLTAADRPGLLYTVASTLAEHGANLHTAKIVTLGERAEDVFLISGGDI
                     SDTNRRIRLETELMERLKV"
BASE COUNT          622 a         1241 c         1440 g          742 t
ORIGIN      
        1 cggtgtgcgc tacgattgcg gctccaaact gggttacctg aaggcgacgg tcgctttcgg
       61 ccggcgccat cccgaggtcg gtgccgattt cgccgcctat ctcgacggcg agccgggatg
      121 agcggcgcca agctcgacag ttttctcgac tactgggaaa gcgagggcga aacactggcc
      181 cggcacggcg actacgactg gatggcggcc ctgacgccgg gacgccgggt cctggaaatc
      241 ggctgcgggc cgggtttttc gacgctggcg ctggtgcagc gcgggctgga tgtgctggtc
      301 gtcgatgccc agcccgcctg cctggcgctg gcccggcaac gcgtcggcgc actgccgctg
      361 gccgtgctgg aagcggaagt gacggcgctg agcgccgaac agcgggcggc gatccttgcc
      421 tttgcgccgg agagcgtcgt ctgctggctg atgggcgcgc cggcggaggt taccggggcg
      481 agcgctggcg acggcggcaa ggcggtcgtc gcctaccgcg aacgcgtgca tcgggcggtg
      541 gccgagctgg cggccgacct gccttcggtg agcgcgctgc atctcgtcga ccggacggcg
      601 attccctggc aggccaagga tatcggtcgc gccacgctgc tcgggtatca tcgccagaaa
      661 accctggccg gcctgccttt ccaggcgcag cgcgaggatg cgctgtaccg taagctggag
      721 gccgcggccc cttccttcaa accgcatgcc gccctcaagg gcgtcgtgcc cgtgctggct
      781 tcgctgctgg cttaccggag ttcctgacat gctggccatc atcggcggca gcggcctgac
      841 gacgctgtcc aatctcgacg tttcgcaccg cgaagtggtg cgcaccccct atggcgaagc
      901 ttccggcgcg ctggtcttcg gccagatctg cggccggccg gcggtctttc tgccgcgcca
      961 cggctacggc cataccattc cgccgcatat ggtcaattac cgcgccaacc tgtgggccct
     1021 gcatcaccac caggtcagtg ggatcatttc cgtggcctcg gtcggcagca tccgtcccga
     1081 cctgcagccg ggcgacatcg tcctgcccga ccagatcatc gattacacct ggggtcgtaa
     1141 atccaccttc ttcgacggtg tcggcagccc ggtgacccat atcgatttca ccgaacccta
     1201 cgatgcggcg ctcgctcggc gcatcgccga agcggcggcg gcgctcggca ttggcgtcgc
     1261 ggtcggcgcc gtctacgcgg cgacgcaggg gccgcgactc gagagcgcgg ccgagatcaa
     1321 ccgcctggag cgcgacgggg ccgacgtcgt cggcatgacc ggcatgccgg aagcggcgct
     1381 ggcccgcgag ttgggcgtgc cttacgcggc gatcaacgtc gtcgccaacc acgccgccgg
     1441 gcgcggcagc agcgccaacg gcatccgctt cgacagcctg gagcacgtgc tgcaggaggc
     1501 gatgggccgg gtcaaggcga tcatcgaacg ggtggtcggt tgcgaggcgg cgtgtccgcc
     1561 gctcggcatg tcggtgtgag gcccgtcggg ctcacacctt caggcgttcc atcagttcgg
     1621 tttccaggcg gatgcggcgg ttggtgtccg agatgtcgcc gccgctgatc aggaagacgt
     1681 cctcggcgcg ctcgccgagg gtgacgatct tcgccgtgtg caggttggcg ccgtgttcgg
     1741 cgagcgtgct ggccaccgtg tatagcaagc cgggacggtc ggcggcggtc agcgagagga
     1801 tgaagtgctt gcccttctcg tcgccgctga tgctgacttc cggcttgatc gggaaatgcc
     1861 ggacctggcg cgacagccgg ccggccgagg ggatttccgg cggcagttcg cgggtcaggc
     1921 ggtcttccag ctcgtgctcg atgtagctga gcatttcccg gttgtgttcg cgccgctcga
     1981 cgtcgagcag gatgaagctg tcgagcgcgt agccgtgctc ggtggtgtgg atcttggcgt
     2041 ctacgatgct gtagccggca cgggcgaaga agccgaccag gcgcgcaaag aggtcgcgct
     2101 ggtcgcgggt gtacaccatc acctgcaggc cgacgccttc ctgccagagg cgggcgcgga
     2161 cgaccggctg gttgttgtgg atacggtaat gcagcgagcg cgtgtgccag gcgatttcct
     2221 cggccgagtg gcgcaggaaa tagacggtgt cgagctgctt ccagagccgt tcatgcacgg
     2281 tgtcggagag cgcgaagaag cgcagcaggc gcatcgcttc ggcctgacgg tcggcgatga
     2341 tgccgtggct gaccgggggc tcgtcatgcg ccaggtggtg cagtgtctgc tggtagaggt
     2401 cggcgagcag cttgcctttc cactggttcc agaccttcgg gctggtgccg cggacatcgg
     2461 cgtgggtcag caggtagagc gcctgcaggt ggcggacatc gccgaccagt gcggcaaaac
     2521 ggccgatcac ctcggggtcg ccgaggtctt ccttctgggc gatctgcgac atcaccaggt
     2581 ggtggcggac cagccagacc accagttcgg tgtcggtttc cgtcaggccg tggttgtcgc
     2641 agaattcgcg ggcatcgacg gtgcctttct ccgagtggtc gccgccgcgt cccttggcaa
     2701 tatcgtggaa gagggcagcg atgtagagca gccagggttc ttcgaactcg ttgatggtgc
     2761 gcgagcagag cgggtattcg tgggcgaatt cgctcatcgt gaagcggcgc aggttgcgca
     2821 gcacctgcag gatgtgctga tcgacggtgt agacgtggaa caggtcgtgc tgcatctggc
     2881 cgacgatgcg cccgaagttc ggcaggtagg cgccgaggat gtcgagctgg ttcatgcgcc
     2941 ggaattcgtg gatgacaccg cgctcggcct gcagcagacc gaggaacatg gcgcggttgg
     3001 cagggtcggc gcgaaagccg tcgtcgatcc gctcgcgggc cagccacagg gcgcgcagcg
     3061 tcgatgccgt catgccctgc agttccgagt tctcctgcat gatgcggaag gcgtcgaaga
     3121 tcagtcccgg ctgacgttcg aagagattct cgtcacagat gtcgagcagg ttgccgacga
     3181 tctggaaatt ggcgtcgtag gcttgcggac gctgcaggtt ttccggggcg agcgccgcgc
     3241 ccatgttctg aaggacgatg gtgttgagca gcatcagtcc tttggcgttg cggtaatagg
     3301 cctgcatcag ctgttcggag gcgcgcttcg attcgctgtc ctgaaagccg taacgggcgg
     3361 cgagcgcgga ctggtagtcg aagagcaggc gatcttcgcg gcggcgggcg atgaaatgca
     3421 ggcggatgcg caggtggcgc aggaaatcct cgaactgctt gccgaaggcg acgccttcct
     3481 gtgacatcac gccgcccttg ccgagttcgt cccaggtgtc gccgaaaccc gccgccttgg
     3541 caatccagaa gatcacctgc tggtcgcgca gcccgcccgg cgattccttg aggttgggtt
     3601 ccaggctgta cggcgtttca ttgtgccgca ggtaacggtc ctgctgttcg aggcgtttgg
     3661 cttcgaaaaa gccgagcggg tcgaggttgc cgaacaggcg cttgcggaag gtggcgaaca
     3721 gtttttcgct gccggtcagc aggcgggctt cgagcagcgc ggtctgcacc gtgatgtcgc
     3781 gtgttgcctc gtccaggcat tccgggatgg tgcgcacgct atggccgatt tccagcccga
     3841 tgtcccagaa gcagccgacc aggcgttcca gtttttccgc cgtgtttgcg ctcggcgcct
     3901 gcggcagcag gatcagcagg tcgacgtccg aatgcggaaa cagttcgcca cggccatagc
     3961 cgcctaccgc gatcaggcaa acttgcgggc tgatgccggc ggcctgccat agatcctgca
     4021 gcaggacatc gaccagccgg ctgtg
//
LOCUS       DAOSDX010000152         4000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7942_length_4000_cov_3.041572, whole genome shotgun sequence.
ACCESSION   DAOSDX010000152 DAOSDX010000000
VERSION     DAOSDX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7942_length_4000_cov_3.041572"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PLN02_04445"
     CDS             complement(<1..1524)
                     /locus_tag="PLN02_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51602.1"
                     /translation="MTPHSHRPTPVRHRLLSLLLSATIALAPLSSQAGVDIAQQPLSV
                     ADPVPPNIMFIMDDSGSMAWQHMPGTTATWSAAPPSGLPYSSDLTNDIRLRASNVNSQ
                     WYNPLITYEPWLKGHSELNPTGKDYSVDASLSGALKDPSGQTETGTFDFQGELSDWPT
                     ISTSDTGNYTSSWRYSGFYLLNPNASATNDQNYVRYEFRYEQICTQTQRQCNRWSCWD
                     VCVAYQSSWKARKVTLTSSGGNSTITNLTQFDWTAHGGPVRTIQQEIQNYANWYSYYR
                     LRATMAKASASRVFAKLGTGYRIGYNTIWNLQNFPIPVGNENGLFRGDNKKSWFEKLF
                     STGSDNGTPLRQALGRAGEYFTDTASTGPYGPETGSQQVSCRQNFAIMTTDGYWNSNA
                     AGNSDARADNDSTSGSTINGLNGKTYKYVAGLPYKDGRSNTLADVAMYYWKTDLVPDL
                     VNNVPTTAKNPAFWQHMRTFGISIGEKGTLDPKTDLPALTAGTKTWPAPGCDQSGWSC
                     "
     gene            complement(1568..2137)
                     /locus_tag="PLN02_04450"
     CDS             complement(1568..2137)
                     /locus_tag="PLN02_04450"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HOI51603.1"
                     /translation="MPAQQGGIALIMAIIMLIVATLAGIAGIRGSSLQEKLSGNLYDR
                     AIALQAAEAALKAAELRIVSTDRANLLKDSKIVDCTQAATSCPPIPTNAFNNDANGWT
                     ALATTTNSSLKADTFPQYFIQYLGLKNSNAVTDTSQSAIPLQYGATGGGSGSDSPQNA
                     TYRVIARSSQPTTSNDRSIALVSSLIKGN"
     gene            complement(2175..3140)
                     /locus_tag="PLN02_04455"
     CDS             complement(2175..3140)
                     /locus_tag="PLN02_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI51604.1"
                     /translation="MKPAHKIALPRRMRGLSLVEVMVTVAIGLVITLGVVGVVGVNQQ
                     NLRITEGLSESQENARMAFELIARDVRQARDTGCGPVTASRVTDLDTTWWGNWYPIRG
                     FDGTTGAPGVTTGSATGQRVSGTEALQLQGTGEAVVIGSFTGLNKVTLKTSSHSLRAG
                     TVVICDMDNASLHNVTAVSGADVTFSPNIVISDPDNPQFQAAQYVATTWYIGNNGRAA
                     EGGRSLYRVRRTPAGTTLTEEILPGVVDMNISYHRVGQANFAATPPTSEADSDLINAI
                     ELELVTETTQSSVATEAANADAELVGSDGRLRRTVTHVIAIRNTL"
     gene            complement(3137..3595)
                     /gene="pilV"
                     /locus_tag="PLN02_04460"
     CDS             complement(3137..3595)
                     /gene="pilV"
                     /locus_tag="PLN02_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HOI51605.1"
                     /translation="MKRSIYPRQTALPLRQQGVSLLEVMIAMLVLSFGLLGLASLQMA
                     TLRNNQSSFDNSRAIMSIYSITDIMRADMSSQGALNMANAFPAGRKADWLSDLKSNLG
                     DDATGTIQCTPTTTTPILGDPFISNICTVTISWTDVQGGTTRTMTTEVQL"
     gene            complement(3596..>4000)
                     /locus_tag="PLN02_04465"
     CDS             complement(3596..>4000)
                     /locus_tag="PLN02_04465"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HOI51606.1"
                     /translation="IVQSRLSAQANDLVSAIQAARGEAIKRNLRVDFCRTSSATSTDC
                     AGAGSWEFWIARINTTATVLRRGSINPELRLSSTFNQGRITFLPDGTSTITAGSDTLT
                     ACSPTGSGNTTRVITVGIAGRTSVTKTTGSCS"
BASE COUNT          661 a         1053 c         1359 g          927 t
ORIGIN      
        1 acaactccag cccgactgat cgcaacccgg cgcgggccag gtctttgtgc cggcggtcaa
       61 ggcgggaaga tcggttttgg gatcaagcgt ccctttttcg ccaatggaaa taccgaaggt
      121 gcgcatgtgc tgccagaatg ccggattctt ggccgtggtc gggacattgt tgaccagatc
      181 cggcaccagg tctgtcttcc agtagtacat cgcaacatcg gccagtgtgt tggagcggcc
      241 gtccttgtag ggcagtcccg ctacgtactt gtaagtcttg ccattcaagc cgttgattgt
      301 gcttccgctg gtgctgtcgt tgtctgctct ggcatcggag ttgcctgccg cgttactgtt
      361 ccagtaaccg tcggtcgtca tgatcgcgaa gttctggcgg caggaaacct gctggctacc
      421 cgtttctggt ccgtaagggc cggtgctggc ggtatctgtg aaatattctc cagcgcgacc
      481 gagtgcttgt cgcaagggcg tgccattatc gctgccagtg ctgaatagtt tttcaaacca
      541 ggacttcttg ttgtcgccac ggaaaaggcc gttttcgttg ccgaccggga ttgggaagtt
      601 ctggagattc cagatcgtgt tgtagccaat gcggtagccc gtgcccagct tggcgaaaac
      661 ccggctggcc gatgccttgg ccattgtggc gcgtaggcgg taataggagt accagttggc
      721 gtagttctgg atttcctgct ggattgtgcg taccggaccg ccatgtgccg tccagtcgaa
      781 ctgagtgaga tttgtgatgg tgctgttgcc gccactgctg gtcagcgtca ctttgcgagc
      841 tttccaggag ctttggtagg cgacgcagac gtcccagcag ctccagcgat tgcactgccg
      901 ttgagtctgg gtgcaaatct gttcatagcg gaattcgtaa cgcacgtagt tctgatcgtt
      961 ggtcgcgctg gcgttggggt tgaggaggta aaaaccgcta tagcgccaac tgctcgtgta
     1021 gttgccggta tcgctcgtgc tgatcgttgg ccagtcgctg agttccccct gaaaatcgaa
     1081 agtgccggtt tcggtttgtc cggaggggtc tttcaatgca ccggagagac ttgcgtccac
     1141 agaataatct ttgcccgttg gattcagctc ggaatgacct ttcagccaag gttcataagt
     1201 gatcagcggg ttgtaccatt gcgagttgac attgctggcg cgcaggcgaa tgtcgttggt
     1261 cagatcgctc gagtagggca ggccgctggg aggggcggcg ctccatgttg cagtcgttcc
     1321 gggcatgtgc tgccaggcca tcgatcccga atcgtccatg atgaacatga tgttcggcgg
     1381 tacggggtcg gcaacggata gcggttgctg cgcgatatcg acgccggctt gagaggacag
     1441 cggggccagc gcgatcgtcg cggagagcag caaggacaaa aggcgatggc ggacaggtgt
     1501 cgggcggtgg ctgtgcggtg tcatggtttc acctggtctg gctggaattc gatgagataa
     1561 gcatggctca gttacctttg atcaaactgc tgaccagggc aatcgagcgg tcgttgctag
     1621 tcgtgggctg gctgctgcgg gcgatgacgc ggtaggtggc gttctgcggc gagtcgctgc
     1681 cgctgccgcc gcccgtggca ccgtattgca aggggattgc cgactggctg gtgtcggtga
     1741 cggcattgct gtttttcagg ccgaggtact ggatgaaata ctgcgggaaa gtatcggctt
     1801 ttaggctgga attggtggtt gtggcgaggg ccgtccagcc gttggcgtcg ttgttgaatg
     1861 cattggtcgg gatgggcggg caactcgtgg cggcctgggt gcagtcgaca atcttgctgt
     1921 ccttaagcag gtttgcccgg tcggtcgaga caatccggag ttccgccgcc ttcagggcgg
     1981 cttccgccgc ttgcagggcg atcgcgcggt catagaggtt gccggaaagc ttttcctgta
     2041 gcgaactgcc gcgtatgccg gcgatgccgg ccagggtggc gacgatcagc atgatgatgg
     2101 ccatgatcag ggcgatgccg ccctgctgtg ccggcatgcg atgggtgcgg gaaggtggat
     2161 tcatattgcg catgtcagag ggtgtttctg atggcgatga cgtgggtcac ggtacgccgg
     2221 aggcggccgt cgctgccgac cagttctgca tcggcattgg ccgcttcggt ggcaacgctg
     2281 ctttgggtgg tttcggtaac cagttcaagc tcgatggcgt tgatcagatc gctgtcggct
     2341 tcgctggtcg gtggcgtcgc agcgaagttg gcttgtccga cgcggtggta actgatgttc
     2401 atgtcgacca cgccgggaag gatttcctcg gtcaaggtgg ttccagcggg cgttctacgg
     2461 acccgataca gggagcgccc tccttcggcg gcgcggccgt tgttgccgat gtaccaggtg
     2521 gtcgccacgt attgggcggc ctggaactgg gggttgtccg ggtcggagat gacgatgttc
     2581 ggggagaagg tgacgtcggc cccggaaact gccgtgacgt tgtgcaggct ggcgttgtcc
     2641 atgtcgcaga tgacgaccgt tccagccctc aatgaatggc tgctggtctt cagcgtgact
     2701 ttgttcagtc ccgtgaaact gccgatcaca accgcttcac ccgtgccttg cagctgcaag
     2761 gcttcggtgc cggagacccg ttgtccggtg gcgctgccgg tggtgacgcc aggggcgccg
     2821 gtggtgccgt caaagccgcg gatcggatac cagtttcccc accacgtggt gtcgaggtcg
     2881 gtgaccctgc tggcggtaac cggcccacaa ccggtatccc tggcctggcg gacatcgcgg
     2941 gcgatcagct cgaacgccat gcgggcattt tcctgggatt ccgaaaggcc ttccgtgatg
     3001 cgcaaattct gctggttgac gccgacgaca ccgaccacgc cgagcgtgat gaccagtccg
     3061 attgccaccg ttaccatgac ttcgaccagc gaaaggccgc gcatccggcg aggcagggcg
     3121 attttgtgtg ctggcttcat agctgcacct ccgtcgtcat cgtccgcgtc gtgccgcctt
     3181 ggacgtcggt ccagctgatc gtgacggtgc agatgttgct gatgaagggg tcgccgagga
     3241 tcggtgtcgt tgttgtcggg gtgcactgga tggtgccggt ggcgtcatcg ccgagattgc
     3301 tcttcaggtc gctcagccag tcggccttgc ggcccgcagg aaaggcgttg gccatgttca
     3361 gtgcgccctg gctcgacatg tccgcgcgca tgatgtcggt aatgctgtag atactcatga
     3421 tcgcccggct gttgtcgaac gaactctggt tgttgcgcag tgtcgccatc tgcaagctgg
     3481 ccaatccgag caggccgaaa gagagaacca gcatggcgat catgacttcc agcaggctga
     3541 cgccttgctg ccggaggggc aatgcggttt gccgggggta gattgagcgt ttcatttagc
     3601 tgcaggatcc cgtggttttc gtgacggagg ttctgccggc aatgccgacg gtaatcactc
     3661 gggtggtgtt gccactgccg gtgggggagc aggcggtcag cgtatcgctg cccgcggtga
     3721 tcgtactggt gccgtcaggg aggaaagtga tccgtccctg gttgaaggtg ctggacagtc
     3781 tcaattcagg attgatgctg cctcgacgaa gtaccgtggc ggtggtgttg attcgggcga
     3841 tccagaattc ccagctaccg gcgccagcgc aatcggtgct ggtggcgctg gatgtcctgc
     3901 agaaatcaac ccgcaggttg cgcttgatgg cttcgccccg ggctgcctga atggcgctga
     3961 caaggtcatt ggcctgggcg cttaggcggc tctgcacgat
//
LOCUS       DAOSDX010000153         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7951_length_3994_cov_2.978167, whole genome shotgun sequence.
ACCESSION   DAOSDX010000153 DAOSDX010000000
VERSION     DAOSDX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7951_length_3994_cov_2.978167"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLN02_04470"
     CDS             <1..609
                     /locus_tag="PLN02_04470"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOI51607.1"
                     /translation="ADRYSFGELRISHEQNVILADVKLADLYAVWQQAKAAGLATPNI
                     GLLTSIICCPGGDFCDLANAKSIPIATAIQQRFDDLDYLFDIGEIDLNISGCMNACGH
                     HHVGHIGVLGVDKNNDEFYQVTLGGRQGNDAKLGKVIGPSFSAGEMPDVVEKIIQVYL
                     ENRHPDERFIDTFDRIGIDPFKNRVYEGRAHGKAKVAQKEAA"
     gene            611..1141
                     /locus_tag="PLN02_04475"
     CDS             611..1141
                     /locus_tag="PLN02_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HOI51608.1"
                     /translation="MAQLIKQGALAADTWTTLTLAEGETPDTVALPAGDVIFPLAVWL
                     ARKNEIVSCHKRIGLLLQADDKVEDAAADLDYFVVIAINFPKFVDGRGYSTAALLRQR
                     YHYQGELRAVGDVLHDQLFFMKRVGFDSYALKDGKDAVYAIDQGFSPFSATYQGSTDQ
                     LEPYFRRRTACPDRQP"
     gene            1138..1857
                     /locus_tag="PLN02_04480"
     CDS             1138..1857
                     /locus_tag="PLN02_04480"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOI51609.1"
                     /translation="MTPALLNITPELAASTAAKAEAAAALLAEIAGAWSPAAFANSLG
                     AEDMVLTDLIVKAGLPIEIFSLDTGRLPAETYDLMAKVDQHYGLKLKIYFPQSEEVES
                     YVRQHGINAFYDSVTLRKACCYARKVEPLKRALAGKRAWITGMRAEQAATRGQLATQE
                     YDEGNGLEKFNPLAAWSEKEVWTYIKHNAVPYNALHDKFYPSIGCAPCTRAVSLGEDI
                     RAGRWWWEAPESKECGLHVKA"
     gene            complement(2124..2897)
                     /locus_tag="PLN02_04485"
     CDS             complement(2124..2897)
                     /locus_tag="PLN02_04485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOI51610.1"
                     /translation="MQRSVWLSMLAASLFTLSGCKTTGAGAGGEMSASSAIDIGSSVV
                     AVASLSDEEMLELGKRTAANEDQRNQIAAPRSKYAKRLQKLTANWTKIDKANLDFKVY
                     MKKDVNAFAVPNGSIRVYSGLMDKFTDDELRYVLAHEIGHVMLGHSKRALQLAHASSA
                     ARQAAASSGNGAVAAIAASELGAMGEALINAQFSQKQENEADDYAVDYLAKQKLKNAG
                     AVTALRKLEKLYGNDRSAFSSHPAPGERAKRMEQRIKAK"
     gene            complement(3055..3828)
                     /locus_tag="PLN02_04490"
     CDS             complement(3055..3828)
                     /locus_tag="PLN02_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOI51611.1"
                     /translation="MFPDLFAIPFLTGLCLAVLLPLLGCYLRLRDEWLAALAYAHVAA
                     GGALLAMLAGLPPPVGGVAAAATAALAKEPLAARLPASALFPLLLLAGWAVGMLVAAN
                     HPLAERVGSALFDGQLYFADAGRLATVAAGTLACLLVLRLSRPLLLSQLYPDHFRLRG
                     EAPWPVHSGFDLLAALVLALATTTLGVMATFALVFVPPWFAFRRADDWRRGLRLALLV
                     SVGGYIAAFAMALWFDQPFGPVLAILLILLAGVAACSRS"
BASE COUNT          790 a         1337 c         1257 g          610 t
ORIGIN      
        1 gccgaccgct acagcttcgg cgagctgcgc atcagccacg agcagaacgt catcctggcc
       61 gacgtcaagc tggccgatct ttacgccgtc tggcaacagg ccaaggcggc cggtctggcg
      121 acgcccaaca tcggcttgct gaccagcatc atctgctgcc cgggcggcga tttctgcgac
      181 ctcgccaacg ccaagtcgat ccccatcgcc accgccatcc agcagcgttt cgacgacctc
      241 gactacctgt tcgacatcgg cgagatcgac ctcaacatct ccggctgcat gaacgcctgc
      301 ggtcaccacc acgtcggcca catcggcgta ctcggcgtcg acaagaacaa cgacgagttc
      361 taccaggtca cgctgggcgg ccgtcagggc aacgacgcca agctcggcaa ggtgatcggc
      421 ccgtccttct cggccggcga aatgccggac gtggtggaga agatcatcca ggtctatctg
      481 gagaaccgtc atccggacga acgtttcatc gacactttcg accggatcgg catcgacccg
      541 ttcaagaacc gcgtctacga aggccgcgcc cacggcaagg ccaaggttgc acaaaaggag
      601 gccgcataac atggcacaac tgatcaagca gggcgcgctc gccgccgata cctggacgac
      661 gctgaccctg gccgaaggcg agacgcccga caccgtcgcc ctccccgccg gcgacgtcat
      721 cttcccgctc gccgtctggc tggcgcgcaa gaacgagatc gtctcctgcc acaagcgcat
      781 cggcctcctg ctgcaagcgg acgataaggt cgaagacgct gctgccgacc tcgattattt
      841 cgtggtcatc gccatcaact tcccgaagtt cgttgatggt cgcggctact cgaccgccgc
      901 cctgctgcgc cagcgctacc attaccaggg cgaactgcgc gccgtcggcg acgtgctgca
      961 cgaccagctc ttcttcatga agcgcgtcgg tttcgacagc tacgcgctga aggacggcaa
     1021 ggacgcggtc tatgccatcg accagggatt ctcgccgttc tccgccacct atcagggttc
     1081 gaccgaccag ctcgaacctt acttccgtcg ccgtaccgcc tgccccgatc gccagccatg
     1141 acgccagccc tcctcaatat cacccccgaa ctcgccgcca gcaccgccgc caaggccgag
     1201 gccgctgccg cgctgctcgc cgagatcgcc ggcgcctggt cgccggccgc cttcgccaac
     1261 agcctcggcg ccgaggacat ggtcctcacc gacctgatcg tcaaggccgg cctgccgatc
     1321 gaaatcttca gcctcgacac cggccgcctg ccggccgaaa cctacgacct gatggccaag
     1381 gtcgatcagc actacgggct gaaactgaag atctacttcc cgcagtccga ggaagtcgaa
     1441 agctatgtcc gccagcacgg catcaacgcc ttctacgact cggtgacgct caggaaggcc
     1501 tgctgctacg cgcgcaaggt cgaaccgctg aagcgcgccc tggccggcaa gcgcgcctgg
     1561 atcaccggca tgcgcgccga acaggccgcc acccgcggcc agctggcgac ccaggaatac
     1621 gatgaaggca acggcctgga aaaattcaac ccgctcgccg cctggagcga aaaggaagtc
     1681 tggacctaca tcaagcacaa cgccgtgccc tacaacgccc tgcacgacaa gttctacccc
     1741 agcatcggct gcgccccctg cacccgcgcc gtctcgctcg gcgaagacat ccgcgccggc
     1801 agatggtggt gggaagcacc ggaatcaaaa gagtgtggac tgcacgtgaa ggcgtgacat
     1861 ccggcgccag cgcaggctaa atcgcgcagc gaatgttaag ccccgcaggg gcggccggag
     1921 acaagcaccg gaatccaagg cgtgcggact gcacgtgaag gcgtgagggg aacggtagca
     1981 agagcgtcac gccagacttg atcggagccc cgccacgcga caagccgctt ctcgaaaccg
     2041 ccccgatctt tccgccatcg atgcagggca acaaaaaggg cgccatgccg gcgccctttt
     2101 tgttgctgcg ttcagcgact gacttacttc gccttgatcc gctgctccat gcgcttggcg
     2161 cgttcgcccg gcgccgggtg gctggagaag gcgctacggt cgttgccgta gagtttttcc
     2221 agcttgcgca gcgcggtcac ggcgccggcg ttcttcagct tttgcttggc aaggtaatcg
     2281 accgcgtaat cgtcggcctc gttttcctgc ttctgcgaga actgggcgtt gatcagcgct
     2341 tcgcccatcg ccccgagctc cgacgcggca atcgccgcta ccgcgccgtt gccgctgctg
     2401 gcggcagcct ggcgggcggc cgagctggcg tgcgccagtt gcagcgcccg cttcgaatgg
     2461 ccgagcatga cgtggccgat ttcatgcgcc agcacgtaac gcagttcgtc gtcggtgaac
     2521 ttgtccatca gaccggaata gacgcggatc gaaccgttcg gcacggcgaa ggcattgacg
     2581 tccttcttca tgtaaacctt gaaatcgaga ttggccttgt cgatcttggt ccagttggcg
     2641 gtgagtttct gcaggcgctt ggcgtacttg ctgcgcggcg cggcaatctg gttgcgctga
     2701 tcttcgttgg cggcggtgcg cttgcccagt tcgagcattt cctcgtcgct gaggctggcc
     2761 acggcgacca ccgaagaacc gatgtcgatg gcgctgctcg ccgacatctc accgccggcg
     2821 ccagcgccgg tcgtcttgca accggacagg gtgaacaggg aagcagcgag cattgacagc
     2881 cagacggagc gttgcatttt ttcttacctc gggttgaaaa accggcaatg gtactcgcgc
     2941 gaggcatgcc attcaagttt ttataaccgt acgtactatt cgaatggcaa taaatcgttg
     3001 aaaaaataca ctaatcagcc aatttattca gaccgcccgc tttccgcgaa cgcctcacga
     3061 gcgagagcaa gcggcaacac cggcaagaag aatcagcagg atcgccagca ccgggccgaa
     3121 gggctggtcg aaccacaggg ccatggcgaa ggcggcgatg tagccgccga cactgacgag
     3181 cagcgccaga cgcagaccgc gccgccagtc gtcggcgcgc cggaaggcga accagggcgg
     3241 gacgaagacc agggcgaagg ttgccatcac gcccagggtc gtcgtcgcca gcgccagcac
     3301 cagcgcggcg agcagatcga agccgctgtg caccggccat ggcgcttcgc cgcgcaggcg
     3361 aaagtgatcc gggtagagct ggctgagcag cagcgggcgg gacaggcgca agaccagcag
     3421 gcaggccagc gtaccggcgg ccacggtagc caagcggcca gcgtcggcga aatagagctg
     3481 gccgtcgaaa agcgcgctgc cgacgcgctc ggcgagcgga tggttggcgg cgaccagcat
     3541 gccgacggcc caaccggcga gcaacaacag tgggaagagc gcgctcgccg gcaagcgggc
     3601 ggcgagcggc tccttggcca gggcggcggt ggcagcggcg gcaacgccgc cgaccggcgg
     3661 cggcaggccg gccagcatgg cgagcagcgc accacccgcg gcgacgtggg cgtaggcgag
     3721 cgccgccagc cattcgtcgc gcaggcgcag gtagcagccg agcagcggca gcaagaccgc
     3781 caggcaaagg ccggtcagga agggaatggc gaacaggtca gggaacatcg ggcgcttcca
     3841 ggcgaatcac cgcgtcacaa gccgcagcaa ggaaggcggc gtcgtggctg acaacgagga
     3901 tggcgcagcc ggcggcgaca cgctgccgca gggccaggct gagatgagcg acgccggcgg
     3961 cgtcgaggtt gttggtcggc tcgtcgagca gcag
//
LOCUS       DAOSDX010000154         3993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7955_length_3993_cov_3.277298, whole genome shotgun sequence.
ACCESSION   DAOSDX010000154 DAOSDX010000000
VERSION     DAOSDX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7955_length_3993_cov_3.277298"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..305
                     /locus_tag="PLN02_04495"
                     /pseudo
     CDS             60..305
                     /locus_tag="PLN02_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
     gene            450..1100
                     /locus_tag="PLN02_04500"
     CDS             450..1100
                     /locus_tag="PLN02_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOI51612.1"
                     /translation="MARRAARELRDGYYVNLGIGIPTLVANHIPAGMRVTLQSENGLL
                     GIGPFPPPDAIDADLINAGKQTISSLPGSSFFSSADSFAMIRGGHIDLSILGALEVSQ
                     YGDLANWMVPGKMVKGPGGAMDLVSGVGRVIVLMEHCAKDGSPKILAQCTLPLTGRGV
                     VDMIITDLAVFAVGDDGLTLIELQPGATLAEVRDKTACPFAVAPGVSELAPEEAAA"
     gene            1097..1477
                     /gene="pcaC"
                     /locus_tag="PLN02_04505"
     CDS             1097..1477
                     /gene="pcaC"
                     /locus_tag="PLN02_04505"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HOI51613.1"
                     /translation="MIDPDYRRGLAIRRQVMGAAHVDRALGEVSDFAAPLQEFITRSA
                     WGSIWSRPGLDLKSRSLVTLAFLTAQGRSHELAGHLRGARNNGASIEEIREVLLQAAV
                     YCGVPLAAEALRVAESVLLAGEAG"
     gene            complement(1486..3174)
                     /gene="recJ"
                     /locus_tag="PLN02_04510"
     CDS             complement(1486..3174)
                     /gene="recJ"
                     /locus_tag="PLN02_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOI51614.1"
                     /translation="MTRIVTRSAPPRTVWQLEQQGLHPLLARLYAARGVTDKAELDYE
                     LKSLLPPAALTHATDAASLLADAIEAEAKLLIIGDYDCDGATATAVGMRALKALGADV
                     DCLLPARFKLGYGLSPEIVDVAAQQSPDLIITVDNGIASLEGVARAQQHGIATLITDH
                     HLPAATLPDADCIVNPNQPGCDFPSKCIAGVGVMFYVMLALRAELRERGFFTDRPEPN
                     FADLLDLVALGTVADVVKLDRNNRILVSQGLKRMRAGRLQPGIAALFKAAGRDPRRAT
                     AFDLGFLIGPRLNAAGRLADMRLGVECLLTDDPARALNIAQELDNLNRERREIESGMQ
                     EQALALLEKMDIEAAAPGVALFDPGWHEGVVGILASRIKDKLHRPVFAFAQSEGDIVK
                     GSGRSIPGLHLRDALDLVAKRAPGLLVRFGGHAMAAGATIKAGDFERFQTLFAEVAGE
                     LLAPSDLTRTLETDGPLEGGYISLETAKLLENEIWGQGFPAPLFCDEFEVEQQRVLKE
                     KHLKLRLKKGNSRIEAIQFNFASQPGYNTRAAYRLSVNEYMGVETPQLMIEYLE"
     gene            complement(3171..>3993)
                     /locus_tag="PLN02_04515"
     CDS             complement(3171..>3993)
                     /locus_tag="PLN02_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51615.1"
                     /translation="DANQEDGHWLCADPVHLRFHHERIVLADAGAFDIDAAEAQALVG
                     ALNAEFAEIGVFHAATPRRWYLRLHAAVDHVAAPLSAVAGRRMDSDLSDKTSPLHRWL
                     NEVQMFLHLHPVNAARAAAGKPAINSLWLWGNGPAVTPTGSPDALFADDPLARGLARA
                     ANVAVQALPANADAVLDSGAQRPLVVVDTLLPTVLYEDPDGWRRACERFDADWLAPIQ
                     KRLGKTVRELVVVAPTIYGRLQWTLAAGERWKFWKSGRSPQQVALALAGPQGKAS"
BASE COUNT          583 a         1313 c         1463 g          634 t
ORIGIN      
        1 cctccggcga ggaggtcatc caccatctgg tcgagcgcct gcgcgccggc ggcgccggca
       61 tccccggctt ctacacccgc acggcggccg gcacgctgct cgccgagggc aaggagacgc
      121 gccgtttcga cggccgcgaa tacgtacttg aaccggcgat ccgcgccgat gtcgccatcg
      181 tcaaggcatg gaagggcgac cgcgacggca acctggtctt tcgcaagacg gcgcgcaact
      241 tcaacccgat gatcgccacc tgcggccgcc tgaccgtggc cgaggtcgag gaactggtcg
      301 aaccgggcga acttgacccc gaccacatcc atacgcccgg catctacgtc gaccgcatcg
      361 tccagggcga gcgctacgag aagcgcatcg agttccgcac cgtcgccggc gccgccgcac
      421 cggcccgcgc cgacccggtg cgcgacgcga tggcccggcg ggcggcgcgc gaactgcgcg
      481 acggttacta cgtcaatctc ggcatcggca tcccgaccct ggtcgccaac cacatcccgg
      541 ccggcatgcg cgtcaccctg caatcggaga acggcctgct cggcatcggt ccctttccgc
      601 cgcccgacgc catcgacgcc gacctgatca acgccggcaa gcagaccatc agcagcctgc
      661 cgggcagcag cttcttttcc agcgccgact ccttcgcgat gatccgcggc ggccatatcg
      721 acctctcgat cctcggcgcg ctggaagtct cgcagtacgg cgacctcgcc aactggatgg
      781 tgccgggcaa gatggtcaag gggccgggcg gcgccatgga cctggtgtcc ggcgtcggcc
      841 gcgtgatcgt gctgatggag cactgcgcca aggacggttc gccgaagatc ctggcgcaat
      901 gcacgctgcc gctgaccggg cgcggcgtcg tcgacatgat catcaccgat ctcgccgtgt
      961 tcgccgtcgg cgacgacggt ttgaccctga tcgaactgca gcccggcgcg acgctggccg
     1021 aggtgcgcga caaaacggcc tgtccgttcg ccgtcgcgcc cggggtgtcc gaactggcgc
     1081 cggaggaggc agcggcatga tcgatcccga ttatcggcgc ggtctcgcca tccgccgcca
     1141 ggtgatgggc gccgcccatg tcgatcgcgc cctcggcgaa gtcagcgact tcgccgcccc
     1201 gctgcaggag ttcatcaccc gcagcgcctg gggcagcatc tggagccggc cggggctgga
     1261 cctgaaaagc cgtagcctgg tcaccctggc tttcctcacc gcgcaaggcc gcagccacga
     1321 actggccggc cacctgcgcg gcgcccgcaa caacggcgcc agcatcgagg aaatccgcga
     1381 agtcctgctg caggccgccg tctactgcgg cgtcccgctc gccgccgaag cgctgcgcgt
     1441 ggcggaaagc gtgctgctgg ccggcgaggc ggggtagggg cggtcctact ccagatactc
     1501 aatcatcaac tgcggcgtct cgacgcccat gtattcgttg accgacagcc ggtaggcggc
     1561 gcgggtgttg tagccgggct ggctggcgaa gttgaactgg atcgcctcga tccggctgtt
     1621 gcccttcttc aggcgcagtt tcaggtgttt ttccttgagc acgcgctgtt gctcgacttc
     1681 gaactcgtcg cagaacagcg gcgccgggaa gccctggccc cagatttcgt tttccagcag
     1741 cttggcggtt tccaggctga tgtagccacc ttcgagcggg ccgtcggttt ccagggtgcg
     1801 ggtgaggtcg gacggggcga ggagttcgcc ggcgacttcg gcgaagagtg tctggaagcg
     1861 ctcgaaatcg ccggctttga tggtcgcgcc ggcggccatg gcgtggccgc cgaagcggac
     1921 gagcaggccg ggggcgcgct tggcgacgag atcgagcgcg tcgcgcaggt gcaggccggg
     1981 gatcgagcgg ccggagccct tgacgatgtc gccctcgctt tgcgcgaagg cgaataccgg
     2041 ccggtgcagc ttgtccttga tgcgcgaggc gaggatgccg acgacgcctt cgtgccagcc
     2101 ggggtcgaag agggcgacgc cgggggcggc ggcttcaata tccatcttct cgagcagcgc
     2161 cagcgcctgt tcctgcatgc cggattcgat ttcgcggcgt tcgcggttga ggttgtcgag
     2221 ttcctgggcg atgttgagtg cccgtgccgg atcgtcggtg agcaggcatt cgacgcccag
     2281 gcgcatgtcg gcgaggcggc cggcggcgtt caggcgtggc ccgatcaaaa agccgaggtc
     2341 gaaggcggtg gcgcggcgtg ggtcgcgccc ggccgccttg aacagcgccg cgatgcccgg
     2401 ttgcaggcgg ccggcgcgca tgcgcttcaa accctggctg accaggatgc ggttgttgcg
     2461 gtcgagcttg acgacgtcgg cgacggtgcc gagcgccacg aggtcgagca ggtcggcgaa
     2521 gttgggttcg gggcggtcag tgaaaaagcc gcgttcgcgc agttcggcgc gcagcgccag
     2581 catcacgtag aacatcacgc cgacgccggc gatgcacttc gacgggaagt cgcagccggg
     2641 ctggttcgga ttgacgatgc agtcggcgtc gggcagcgtt gccgccggca ggtggtggtc
     2701 ggtgatcagc gtggcgatgc cgtgctgctg ggcgcgggcg acgccttcga ggctggcgat
     2761 gccgttgtcg acggtgatga tcaggtcggg cgattgctgc gcggcgacgt cgacgatctc
     2821 cggcgacagc ccgtagccga gcttgaagcg ggctggcagc aggcaatcga cgtcggcgcc
     2881 gagcgccttc agcgcgcgca tgccgaccgc ggtggcggtg gcgccgtcgc agtcgtagtc
     2941 gccgatgatc aggagcttcg cttcggcctc gatggcgtcg gcgagcaggc tggcggcgtc
     3001 ggtcgcgtgg gtcagtgcgg ccggcggcag cagcgatttc agctcgtagt cgagttccgc
     3061 cttgtcggtg acgccgcgcg cggcgtagag ccgggcgagc aggggatgca ggccttgctg
     3121 ttcaagttgc cagacggtgc gcggcggcgc gctgcgggtg acgatacggg tcaagatgct
     3181 tttccttgcg gcccggccag cgccagggcg acttgttgcg ggctgcggcc ggatttccag
     3241 aacttccagc gctcgccagc ggcgagcgtc cactgcaggc ggccgtagat ggtcggcgcg
     3301 acgacgacga gctccctgac ggtcttgccg agacgcttct ggatcggcgc cagccagtcg
     3361 gcgtcgaagc gttcgcaggc gcgtcgccag ccgtccgggt cttcgtagag gacggtcggc
     3421 agcagcgtgt cgacgacgac cagcggccgt tgcgcgccgc tgtcgaggac ggcgtcggcg
     3481 ttggccggca gcgcctgcac ggcaacgttg gcggcgcggg cgaggccgcg cgccagcggg
     3541 tcgtcggcga agagcgcgtc cgggctgccg gtcggcgtca ccgccgggcc gttgccccac
     3601 agccagaggc tgttgatcgc cggcttgccg gcggcggcgc gcgcggcgtt aaccgggtgc
     3661 aggtggagga acatctgcac ctcgttcagc cagcggtgca gcggcgaggt cttgtcgctc
     3721 aggtcgctgt ccatgcgccg gccggcgacg gcggagagcg gcgcggcaac gtgatcgacc
     3781 gcggcatgca ggcgcaggta ccagcgccgc ggcgtcgccg cgtggaagac gccgatctcg
     3841 gcgaactcgg cattgagcgc gccgaccagc gcctgcgctt cggcagcgtc gatgtcgaag
     3901 gcgccagcgt cggcaaggac gatgcgttcg tggtgaaagc gcaggtgcac cgggtcggcg
     3961 cacagccagt ggccgtcttc ctggttggcg tcg
//
LOCUS       DAOSDX010000155         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7974_length_3986_cov_3.053930, whole genome shotgun sequence.
ACCESSION   DAOSDX010000155 DAOSDX010000000
VERSION     DAOSDX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7974_length_3986_cov_3.053930"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PLN02_04520"
     CDS             complement(<1..1529)
                     /locus_tag="PLN02_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HOI51616.1"
                     /translation="MSLFRQLWLAVIAITTLAFAGSFVVSMVTARDYLEQQLAIKNND
                     NAATLALSMSQMEKDPVTVELQVAAVFDSGQYALVRVRDPEGKTMIEKTSPPSVGEVP
                     TWFVSLFPIHSPPGQAQVSSGWSQFGTIELVSHSRFAYRELWQGAFRLLLWFGFGGVA
                     AGLLGTQILRRIKRPLDAVVGQAQAIGERRFIRIAEPATPELKSLARAMNSMVMRVKA
                     MFEEEAARLEQVRREATQDPLTGLANRGYFMNQLAAALSDDDAPAQGSLIMLRLADLA
                     GLNRRAGRQTADEVLRQIGSTLSKLCEGHPRAAAARLNGSDFALLLPGESDPTGASNS
                     LLELLRNLVACGQIDGQQVGHLGNGVYHHGENLPGLLSRIDAAIAAAEGEGGIACRSA
                     QGEAVQCAHNSADWRKLLESAIESHRLRLVDFPVNQSTGDLLHLECPLRLQTTENGEW
                     LAAGSFMPVASRLAMTCELDLVAVSLALDRLADGTTAVAVNLSGESVLDANFRLRLEK
                     L"
     gene            complement(1538..2200)
                     /locus_tag="PLN02_04525"
     CDS             complement(1538..2200)
                     /locus_tag="PLN02_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HOI51617.1"
                     /translation="MNRPRLRLPILVLLAWIGAGSAFGLNLERIQQSFSARFGQISEI
                     IFGEWRQMLSGASHGGERDKVEQVNDFFNRRIAFDDDFSLWGQRDYWATPLETLAQGR
                     GDCEDFSIAKYFSLLELGVPVNKLRLVYVRATQEIAGGGGSIQQAHMVLAYYPKPNAD
                     PLVLDNLNKRILPASQRHDLSPVFSFNSAGLWQGTGNQASGSNLSRWQDLLARARAEG
                     FQ"
     gene            complement(2213..2842)
                     /locus_tag="PLN02_04530"
     CDS             complement(2213..2842)
                     /locus_tag="PLN02_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOI51618.1"
                     /translation="MKLTSTSFADGQRIPGDHAFCMPDPAHHVCLGRNRNPQLSWQDA
                     PADTRSFVLLCHDPDVPSQGDDVNQEGRTIPATLARVDFFHWVLIDLPATLTSLGEGE
                     FSHAVTPRGKPGPQAAHTTRQGVNNYTDWFAGDHDMNGDYYGYDGPCPPWNDEIVHRY
                     IFTVFALDIEKLPLQGRFGGPEVRTAMQGHILAEASLTGTYTLNPSLSG"
     gene            complement(2858..>3986)
                     /gene="murJ"
                     /locus_tag="PLN02_04535"
     CDS             complement(2858..>3986)
                     /gene="murJ"
                     /locus_tag="PLN02_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOI51619.1"
                     /translation="SLVALAGGILNSWSRFALPAFTPVLLNLSFIAMALFAAPYFDPP
                     VLALGWAVFIGGLLQLLIQFPALRKISMLPRPTLRWRAAWSDPGVRRITRLMLPALLG
                     VSVAQISLLLNTIFASFLQTGSVSWLYYADRLMEFPSGMLGAALGTILLPSLSRSHAR
                     RDTDEYSRLLDWGLRLTFLLAAPAALALAILAVPLVATLFYHGAFTPDDVEQTRRALI
                     AYSVGLTGLILVKVLAPGFYAQQNVRTPVKIALISLVATQAMNLAFIGWIAHAGLALS
                     IGLAACLNAGLLFRGLRQREIYTPQAGWTGFFLRLSAALTLMGAALWLSMGPAESWLH
                     MNLGARVLYLAVLVVVGATTYFATLWTLGFRLGDFKRRAAE"
BASE COUNT          720 a         1192 c         1404 g          670 t
ORIGIN      
        1 atgagcttct ctaggcgcag ccggaagttg gcatcgagta ccgattcgcc cgacaggttg
       61 actgcgaccg cggtggtgcc gtccgccaga cggtcgaggg cgaggctgac ggcgacgagg
      121 tcgagctcgc aggtcatcgc cagccgcgaa gcgaccggca tgaagctgcc ggcggccagc
      181 cattcgccgt tttcggtggt ttgcaggcgc agcgggcatt cgagatgcag taggtcgccg
      241 gtgctctgat tcaccgggaa atcgaccagg cgcaggcggt gggattcgat cgcgctttcc
      301 agcagcttgc gccagtcggc gctgttgtgg gcacactgca ccgcttcgcc ttgggcgctg
      361 cggcaggcaa tgccgccttc gccctcggcg gcggcgatcg cagcgtcgat gcgggagagc
      421 agcccgggca ggttctcgcc gtggtggtag acgccattgc ccagatggcc gacctgttgg
      481 ccgtcaatct gaccgcaggc caccagattg cgcagcagtt cgagcaagct gttgcttgcc
      541 ccggtcgggt cgctttcgcc gggcaggagg agggcgaagt cggagccgtt gagacgcgcc
      601 gcggcggccc gtggatgacc ttcgcacaat ttactcaggg tgctgccgat ctggcgcagc
      661 acctcgtcgg cggtctgccg gccggcgcgc cggttgaggc cggcgaggtc ggccaggcgc
      721 aacatgatca ggctgccctg ggccggcgca tcgtcgtcgc tgagtgcggc ggcgagctgg
      781 ttcatgaagt agccccggtt ggcgaggccg gtgagcgggt cctgcgtcgc ttcgcggcgg
      841 acctgttcga ggcgggcggc ttcttcctcg aacattgcct tgacccgcat caccatcgag
      901 ttcatcgccc gggccaggct cttcagttcc ggcgttgccg gctcggcgat gcggatgaag
      961 cggcgctcgc cgatcgcctg cgcctggccg accacggcgt cgagcgggcg tttgatccgg
     1021 cgcaggatct gggtgccgag gagaccggcg gcgacgccgc cgaagccgaa ccagagcagc
     1081 aggcggaagg cgccttgcca taattcgcgg taagcaaagc ggctgtggct gaccagttcg
     1141 atggtgccga actggctcca gccggacgac acctgggcct ggccaggtgg cgaatggatc
     1201 gggaagaggc tgacgaacca agtgggaact tcgccgacgc tgggcgggct ggttttttcg
     1261 atcatcgtct tgccttccgg gtcgcgcacc cggaccagcg cgtactggcc gctgtcgaag
     1321 acggcggcga cctgcagttc gacggtgacc gggtcctttt ccatctgcga catcgacagg
     1381 gcgagcgtgg cggcgttgtc gttgttcttg atcgccagtt gctgttcgag atagtcgcgg
     1441 gccgtcacca tgctgacgac gaagctgccg gcaaaggcca gggtggtgat ggcgatgacc
     1501 gccagccaga gttgtcggaa cagggacatg cgttacctca ctggaaacct tcggctcggg
     1561 cgcgcgccag cagatcctgc cagcgggaga ggttgctccc gctggcctgg ttgccggtgc
     1621 cttgccagag gcctgcactg ttgaaactga aaaccggaga aagatcgtgg cgctgcgacg
     1681 ccggcaggat gcgcttgttc aggttgtcga gaacgagcgg gtcggcattc ggcttcgggt
     1741 aataggcgag caccatgtgc gcctgctgga tcgagccgcc accgccggcg atttcctgtg
     1801 tggcgcggac atagaccagg cgcagcttgt tcaccgggac gccgagttcg agcagggaga
     1861 aatacttggc gatcgagaaa tcctcgcagt cgccacgtcc ctgcgccagc gtctcgagcg
     1921 gcgtcgccca atagtcgcgc tggccccaca gggaaaagtc gtcgtcgaac gcgatgcgcc
     1981 ggttgaaaaa gtcgttgacc tgctccacct tgtcgcgctc gccgccgtgg ctggcgccgg
     2041 aaagcatctg ccgccattca ccgaagatga tctcggaaat ctgtccgaag cgcgccgaga
     2101 aggattgctg gatgcgctcc agattgaggc caaacgccga acccgccccg atccaggcca
     2161 gcagaaccag tatcggcagg cgcagccgcg ggcggttcat ggcgcttgcg gcttatcccg
     2221 acagactcgg gttgagagtg taggtgccgg tgagcgaggc ttcggcaagg atgtgcccct
     2281 gcatcgcggt gcgcacttcc ggaccgccga agcggccctg cagcggcagc ttttcgatgt
     2341 ccagcgcgaa caccgtgaaa atgtagcgat ggacgatttc gtcgttccat ggggggcagg
     2401 ggccgtcata accgtaatag tcaccgttca tgtcgtggtc gccggcaaac cagtcggtgt
     2461 agttgttgac cccctgacgg gtggtgtgcg cggcctgcgg accgggcttg ccacgtggtg
     2521 tcacggcgtg gctgaactcg ccctcgccga ggctcgtcag cgtcgccggc agatcgatca
     2581 gcacccagtg gaagaaatcg acgcgcgcca gggtggcggg aatcgtccgt ccttcctggt
     2641 tcacatcgtc gccctggctg ggaacgtcgg gatcatggca gaggaggaca aaggaacggg
     2701 tgtcggccgg ggcatcctgc caactcagtt gcgggttgcg gttgcggccg agacaaacat
     2761 ggtgagcggg gtcgggcatg caaaaagcat ggtcgccggg gatgcgctgg ccatccgcaa
     2821 aacttgtgct ggtcagtttc atgagcttgt ctccagtcta ttcggcggcc cggcgcttga
     2881 agtcgcccag gcggaaaccg agagtccata gtgtagcgaa atatgtcgtt gcgccaacaa
     2941 ctaccagcac ggcgagatac aggacgcgtg cgcccagatt catgtgcagc cagctttcgg
     3001 ctggccccat ggaaagccac agggctgctc ccatgagtgt cagggcagcc gacagccgga
     3061 ggaagaagcc cgtccagccg gcttgcggcg tgtagatttc ccgctggcgc aggccgcgga
     3121 acaataaacc ggcattcagg caggcggcca gcccgatcga cagtgccaga ccggcatggg
     3181 caatccagcc gatgaaggcg aggttcattg cctgtgtcgc gacgagcgag atcagggcga
     3241 tcttcaccgg tgtgcgcacg ttctgttgcg catagaagcc gggagcgagc acttttacca
     3301 ggatcaggcc ggtcagtccg accgagtagg cgatcagcgc tcgtcgcgtc tgctcgacat
     3361 cgtcaggggt gaaggcgccg tggtagaaca gggttgccac cagcggcacg gcaaggatcg
     3421 ccagcgccag cgcggccggt gcggcgagca ggaaagtcag gcgcagtccc cagtcaagca
     3481 gccgcgagta ttcgtcggtg tcccggcggg cgtgcgagcg cgacagcgag ggcaggagta
     3541 tggttcccag cgccgcgccg agcatcccgg aaggaaactc catcaatcgg tcggcgtagt
     3601 agagccagga gacgctgccg gtttgcagga acgaggcgaa gatggtgttc agcagcaggc
     3661 tgatctgcgc caccgaaacg ccgagcagcg ccggtagcat caggcgggtg atgcggcgga
     3721 cgccggggtc gctccaggct gcgcgccagc gcagggtcgg tcgcggcagc atcgagattt
     3781 tgcgcaaagc cggaaactgg atcagcagtt gcagcagacc gccgatgaaa accgcccagc
     3841 ccagcgccag gaccggcggg tcgaaataag gggcggcgaa caaggccatg gcgatgaacg
     3901 agagattcag cagcaccggg gtgaatgccg gtagcgcaaa gcggctccag gaattcagga
     3961 tgccgccagc gagtgcaacc agcgac
//
LOCUS       DAOSDX010000156         3983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_7982_length_3983_cov_3.434572, whole genome shotgun sequence.
ACCESSION   DAOSDX010000156 DAOSDX010000000
VERSION     DAOSDX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7982_length_3983_cov_3.434572"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLN02_04540"
     CDS             <1..493
                     /locus_tag="PLN02_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51620.1"
                     /translation="ATEAINKVPELASFPYYSWHHAIIVPDGHPLLDCRPITLEALAE
                     YPVITYHEGFTGRAHVDAAFADAGVVPDIVLSAIDADVIKTYVGLGLGVGIVASVAYH
                     PEQDRNLQLIEVPHLFPANTTHLAVRRGTYLRSYAHAFIEKVCPDLGEAALRAALKPA
                     AAE"
     gene            707..3331
                     /locus_tag="PLN02_04545"
     CDS             707..3331
                     /locus_tag="PLN02_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI51621.1"
                     /translation="MVASRQSDSPSDPQTCFRAILDRLPFPAWLKDTESRYLAVNEAF
                     VLTFGLAGAESLVGQDDFAIAPFELAEAYRAVDREVINRHQKMVVEEEIYTHGQRLWF
                     ETYKAPFFDANGELLGSIGFARDVSERKQVEVQREKDRERLELALAGGDLGMWDWHIP
                     SGRVLFNERWASMLGESPERLAPHIDSWRSRLHPADRARTKAAWIAHLRRQTPAYESE
                     HRVRHRDGHWIWILDHGKVLEWDAAGRPLRAVGTHLDITVRKENERRLRESEERWRLI
                     HEHTGEAIIFAWPDGRIDTANPAACKLFGLSEEEFRRRGRAGIVDMDDPRLRDGLVER
                     LRTRQARGELTCIHGSGRRFPAEVISTLFVDSKGELRSINQFRDITARKCQESQQEES
                     RQRLELALAGADLGMWDWHLPSGQVSVDARWAAMLGERAEPVVTRADAWEGRLHPDDV
                     DPLQRELKQQLASDAGVFENEYRLRHRTGHWVWILSRGKVIERDGARRPLRVVGTHLD
                     ITRRKEAERLLHDQLHLLRLRDNALSAISQGVLITDAAQRITYANSAFETITGYTLAE
                     IRGRDCNFLQGPDSEPTSRAAIRAALQTRQPFHGELLNYRKDGTPFWNELSITPVFDG
                     EGQASQFVAVLRDVTARKRDEDVLRDQAGRIESLSRHLLATQEEMRRRLAADLHDRTS
                     PNLAAISINLGLLNEALCSSGDDEITARLEDTRALIEDTVASIRDISAELRSSLLDYA
                     GLLPAMESYAQHFRRRTGIVVRIDVAAPPSVLAAELESALFRIFQEALTNVAKHAHAT
                     TVVVGMVQDRCGLSLSISDDGSGFDVGRLGEPSFESGLGLVNMREMAEFSGGSFALES
                     AAGSGTRIFVRIPAAAFA"
     gene            3824..>3983
                     /locus_tag="PLN02_04550"
     CDS             3824..>3983
                     /locus_tag="PLN02_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI51622.1"
                     /translation="MFREALRLLLERVPRLVVVGETGNGSDVLHLAQQSAADIVCMDI
                     GMPGTNGIK"
BASE COUNT          722 a         1222 c         1298 g          741 t
ORIGIN      
        1 cgccaccgag gccatcaaca aggtgccgga actggcgtcc tttccctatt actcgtggca
       61 ccacgcgatc atcgtgcccg acggccatcc gctgctcgac tgccggccga tcaccctcga
      121 agcgctggcc gagtatccgg tcatcaccta ccacgaaggc ttcaccggtc gcgcccacgt
      181 cgatgcggcg ttcgccgacg ccggcgtggt gcccgacatc gtgctttcgg cgatcgacgc
      241 cgacgtcatc aagacctatg tcggcctcgg gctgggcgtc ggcatcgtcg cctcggtcgc
      301 ctaccacccg gaacaggacc gcaacctgca actgatcgag gtgccgcatc tgttcccggc
      361 caacaccacg caccttgcgg tgcgccgcgg cacctacctg cgcagctacg cccacgcctt
      421 catcgagaag gtttgtccgg acctcggcga agcggcgcta cgggccgccc tgaagccggc
      481 ggcggccgag taggcgcgct ctgccggttg ttggtcggcg gcgctcattg cctgctcccg
      541 tcggtcaatt ggtggcaatt gtcgtccctg tcgcggcagg tgcttgaccc tctctgacaa
      601 agccgaaata ctgccgcgga tcgccaagag agggatcgat gatgaacgac agaggcagtt
      661 tccgccggag cgcgtcgcaa caactgcgtg atcaggcgtt ctgaaaatgg tcgcgagccg
      721 gcagtccgat tctccgagcg acccgcagac ctgctttcgc gccattctcg acaggcttcc
      781 ctttccggcc tggctgaagg acacggaaag ccgctatctc gccgtcaatg aagccttcgt
      841 cttgaccttc ggtctggccg gcgcggaaag cctggtcggc caggacgatt tcgccatcgc
      901 cccgttcgaa ctggccgaag cgtatcgggc ggtcgaccgc gaggtgatca acaggcatca
      961 gaagatggtc gtcgaggagg agatatacac gcacggccag cgtctgtggt tcgagaccta
     1021 caaagcgccc ttttttgacg ccaacggcga attgctgggt tccatcggct ttgcccggga
     1081 tgtcagcgag cgcaaacagg tcgaagtgca gcgggaaaag gaccgcgagc gtctggaact
     1141 ggccttggcc ggaggtgatc tcggcatgtg ggactggcac attcccagcg gacgggtcct
     1201 cttcaacgag cgctgggcat cgatgctggg cgagtcgccg gaacgactgg cgccacacat
     1261 cgacagttgg cgcagccgcc tgcacccggc agaccgggcg cggaccaagg ccgcctggat
     1321 cgcgcatctc cgccgccaga caccagccta cgaatcggaa catcgggtcc gtcatcgcga
     1381 cggtcactgg atctggatac tggaccacgg caaggtcctc gaatgggatg ccgcggggcg
     1441 tccgctgcgc gccgtcggta cgcacctcga catcaccgtc cgcaaggaga acgagagacg
     1501 gctccgggaa agcgaggagc gctggcggct gatccacgag cataccggtg aggcgatcat
     1561 ctttgcctgg ccggatggtc gcatcgacac cgccaacccg gcggcgtgca agctgttcgg
     1621 cctgagtgag gaagaattcc gccgtcgcgg gcgagccggg atcgtcgata tggacgatcc
     1681 acgcctgcgc gatggcctcg tcgaacgcct gcgcacgcgt caggcccggg gcgaactgac
     1741 ctgcatccac ggtagcggcc ggcgctttcc cgccgaagtc atctcgaccc tgttcgttga
     1801 cagcaagggc gagttgcgca gcatcaacca gtttcgcgac atcaccgcgc gcaagtgcca
     1861 ggaaagtcag caggaagaga gtcgccaacg tcttgagttg gcgctggccg gggccgatct
     1921 tggaatgtgg gactggcacc tgccgagcgg acaggtctcg gtcgatgccc gctgggcggc
     1981 gatgctcggc gaaagggcgg agccggtggt gacgcgggcc gacgcctggg aaggtcgcct
     2041 gcatccggac gatgtcgatc cgctgcagcg ggaactgaag caacaattgg cctctgacgc
     2101 tggcgttttc gaaaacgagt accggctgcg tcaccgcact ggtcactggg tctggattct
     2161 cagtcgcggc aaggtgatcg agcgggatgg cgcccggcgt ccgctgcgcg tcgtcggcac
     2221 ccacctcgac atcacgcgac gcaaggaggc ggagcggctg ttgcatgatc aactgcatct
     2281 gctgcgtctg cgggacaatg ccctgtcagc catttcgcag ggggtgctga tcaccgacgc
     2341 cgcgcagcgc atcacctatg cgaacagcgc tttcgaaaca atcaccggct atacgctggc
     2401 cgaaattcgt ggccgggact gcaatttcct gcaggggccg gacagcgagc cgacaagccg
     2461 ggcggcgatc cgtgccgccc tgcagactcg ccagccgttt catggcgaac tgctgaacta
     2521 tcgcaaggat ggcacgccgt tctggaatga actgtcgatc acgccggtct tcgatggcga
     2581 gggacaggcc agtcagttcg ttgccgtact gcgcgatgtc acggcccgca agcgtgacga
     2641 agacgtgctg cgcgaccagg cggggcggat cgaatcgctg tctcgacacc tgctggcgac
     2701 gcaggaggaa atgcgccgcc gtctggccgc cgatctgcat gaccggacca gtccgaatct
     2761 ggcggcgatc tcgatcaacc tcggtctgtt gaacgaagcc ctgtgttcca gcggagacga
     2821 tgaaatcacc gctcgcctgg aagacacccg tgccctgatt gaagataccg ttgccagcat
     2881 tcgcgacatc agcgccgagt tgcgctcgtc gttgctcgat tacgccggcc ttcttcccgc
     2941 gatggaaagc tatgctcagc atttccggcg tcgcaccggc atcgtcgtcc ggatcgacgt
     3001 agcggcgcca ccgtcagtgc tggcggcgga actggaatcg gcgctgttcc gtattttcca
     3061 ggaggcactg accaatgttg ccaagcatgc ccacgccacg acggtcgtgg ttggcatggt
     3121 ccaggaccgt tgcggtctga gcttgtcgat cagcgacgac ggtagcgggt ttgacgtggg
     3181 gcgcctcggc gagccctctt tcgaaagcgg tctgggcttg gtcaatatga gggaaatggc
     3241 ggaattctcg ggcggcagct ttgctctcga gtccgccgca ggcagcggga cgcgcatttt
     3301 tgtccggatt cccgcggcgg cgttcgcttg agcggagccg acgccggctc cgccgcgcta
     3361 cgctctccgc aagggcgata agcgctggtt tcgggcgcgc caatccgacg cagcctgggg
     3421 ggcataccgg tatccgggta gttcggctag ggggtttccg ggctaccgca ttccgtggtc
     3481 gatggatatc atcctttgct ctataaataa atcacatctg tcatttgctg ggctttttct
     3541 agatttgttt gccgccggcg agattgacat gtaacagggg cggaactcgg tgagcatcgg
     3601 gggcttgggg ggcgccacga aagtgaggcg cgattggcga aagtgactgt gcggtccggt
     3661 gtggtggaag cctgcttggc tagcgatagc gctgcttttg tccttgtgtc cataagagag
     3721 gaccgtcaac ccgattgcat tacccaccgg tgaggttttt gcggattgag tcgataacaa
     3781 gttcaatatg acaaagcgca tatttctcgc cgatgatcac cggatgttcc gcgaagccct
     3841 gcgtttgctg ctcgaacgcg tgccccgcct ggtggtcgtg ggggagaccg ggaacggcag
     3901 cgatgttctg catctggcgc aacagagcgc agcggatatc gtatgcatgg atattggcat
     3961 gccggggacg aatggcatca agg
//
LOCUS       DAOSDX010000157         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8026_length_3967_cov_3.757157, whole genome shotgun sequence.
ACCESSION   DAOSDX010000157 DAOSDX010000000
VERSION     DAOSDX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8026_length_3967_cov_3.757157"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLN02_04555"
     CDS             <1..476
                     /locus_tag="PLN02_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOI51623.1"
                     /translation="SDTISSAQIADGALSVVGDIMARVIELNKMGGGGAEIAKLTAQA
                     ATIQSDANAAIQTFGGTTFTTVGVAGTETAASALTAVSAERTNHGATMNNAEFAIQAK
                     RAAYENQQAARSRIMDADFAEETSRLSRFQVLQQAGTAMIAQANALPQNVLSLLR"
     gene            557..982
                     /locus_tag="PLN02_04560"
     CDS             557..982
                     /locus_tag="PLN02_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOI51624.1"
                     /translation="MTIESISTNVAAPPISGAMGGAGAAVRQAPAQTSGTSAQTRSAQ
                     TANASSQTTQSVAQAQDRKPQSLSDTPLEDAVKKLTDFVSTIHSEISFSVDEASGTRV
                     VKVLDSQSKEVIRQIPSEEAIQLAQALDKLQGLFVRDKA"
     gene            1012..2367
                     /gene="fliD"
                     /locus_tag="PLN02_04565"
     CDS             1012..2367
                     /gene="fliD"
                     /locus_tag="PLN02_04565"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOI51625.1"
                     /translation="MASYSSLGIGAGVDLQSMLTKIMDAERIPLNMLESKITTTETKL
                     SAWGTLKSKLSTLETSSTTLSFESSLQRRSATAADTSIVSASASYNAAVGSYGIEVTQ
                     LASAQKSFTNEYAAGTTFGQGDLNFTIAGQAKSISLTGQSSYTLEEVANQINENKIGV
                     TATVITTASGTQRMVLSGDDTGASNAFSLDSTMTASGGQSSLGSFDTSTVGLARTDAQ
                     NALMKIDGIDVSSSTNSFSSSITGLTINATKVGSTTLTVATNKDSIVSSVQSFVDAFN
                     AVVDDIKGNSTYDATTKVGGAFSGDSAARGILSSLNEARTTEPAGVADSSFKTLSDLG
                     ISIQSNGKLSLDSTALQEALSENSGDVIKTLNAYGNAFGDVLADFQGSDGIVSNRVNG
                     LTSASAKFADDKEAMELRLELIEKRYRAQFTALDTLVSSMSTTSSYLTQTFAALSSSS
                     S"
     gene            2413..2832
                     /gene="fliS"
                     /locus_tag="PLN02_04570"
     CDS             2413..2832
                     /gene="fliS"
                     /locus_tag="PLN02_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOI51626.1"
                     /translation="MFASSPNAYKQIGQDADILGADPHRLIVLLFDGADAALLKARAC
                     LEQKDIRGRSDALIKAIDIISHGLADSLNLEHGGDLAKNLSALYEYMVSRLIYANIHQ
                     DERAITEVQKLLGEIGGAWREMGDNLRKQAAAGGPKP"
     gene            2892..3137
                     /locus_tag="PLN02_04575"
     CDS             2892..3137
                     /locus_tag="PLN02_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51627.1"
                     /translation="MLNLLRQGNWEAIADQQTALLPALEAVRNPALAREKAISPVQLA
                     SLQEKLQQAIGECRERQAQIAPLVEAFTKNRPPQSSS"
     gene            3134..>3967
                     /locus_tag="PLN02_04580"
     CDS             3134..>3967
                     /locus_tag="PLN02_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51628.1"
                     /translation="MIPPDVASSLRSVLTESQNATQQPQQTQPVAAIQRIADVLGNLV
                     PGQRIMAEIQGMQANGTYRAMVGQREITLSLPFSAKAGDSLELEVTESDGKLALAFVA
                     NRGEPAAKGQPVSVATSLSNTGRMIGDLLQELTVDGKRAPAAPLNGNQALVQSMPKDA
                     AQLVLVLRQALTQSGMFYEAHQARWASGRLPTEQLRQEPQGRLPAPANPQNQGSSPAP
                     TASPPQPAGGPSPAAPGLPGGAAMNSAPTLPGSNGAAPPAPGGSAPAVSGIPTPTAPP
                     LA"
BASE COUNT          898 a         1329 c         1108 g          632 t
ORIGIN      
        1 agtccgacac catttcgtcg gcgcaaatcg ctgacggcgc gctgtcggta gtcggcgaca
       61 tcatggcccg cgtcatcgaa ctgaacaaga tgggtggcgg cggagcggaa atcgccaagc
      121 tgaccgccca ggctgcgacc atccagagcg acgccaacgc cgccatccag accttcggtg
      181 gcaccacctt cacgaccgtc ggcgtggccg gtacggaaac ggcagcctcg gcactgactg
      241 cggtttcggc agagcgtacc aatcacggtg cgacgatgaa caacgccgaa ttcgccatcc
      301 aggccaagcg cgccgcctac gaaaaccagc aggccgcccg cagccggatc atggatgccg
      361 acttcgccga agaaacctcg cgcctgtccc gcttccaggt tctgcaacag gctggtacgg
      421 cgatgatcgc tcaagccaac gcgctcccgc aaaacgtact ttccctgctc cgctaaccag
      481 ttcgggtcag taattttgag gtgtggtggc ttgcaaaagc cccacacctc tttgtgcttt
      541 aactcgggag catgaaatga cgatcgaatc catcagtacc aacgtcgcgg cgccgcccat
      601 ctccggagcg atgggcggtg caggcgcagc cgtcagacag gcgcccgcgc agacgtcggg
      661 tacaagcgcc cagacccggt cggcgcaaac ggcaaatgcc agtagccaga cgacgcagag
      721 tgttgcgcaa gcacaggatc gcaaaccgca gagtctcagc gacacgccgc tcgaagacgc
      781 cgtgaagaaa ctcaccgact tcgtttcgac catccactcg gagatcagct tctcggtaga
      841 cgaagcctcg ggaacgcgcg tagtcaaggt tctcgacagc cagtccaagg aagtgatccg
      901 gcaaatcccc tccgaggaag cgatccagct ggcccaggca ctggacaagc tgcaaggcct
      961 cttcgttcgc gataaagcct gacccgtccg atcttttcgg agtaaaacat catggcaagc
     1021 tatagctcac tcggtatcgg cgcgggggtt gacctgcaaa gcatgctgac caagatcatg
     1081 gacgcggaaa gaattccgtt gaacatgctg gaaagcaaga tcacgacgac cgaaaccaag
     1141 ctttcggcgt ggggaacgct gaagtcgaaa ctgtcgacgc tggaaaccag cagcaccacg
     1201 ctcagtttcg agtccagcct gcaacgccgt tcggccaccg cagccgacac ctcgatcgtt
     1261 tcggccagcg cctcctacaa cgctgccgtg ggcagttacg ggatcgaggt cacgcaactt
     1321 gccagcgccc agaaaagttt caccaacgag tatgccgctg gaacgacctt cggccaaggc
     1381 gacctcaact tcacgatcgc cggccaggcc aaatcgatca gtctgaccgg gcaatcaagc
     1441 tacaccctcg aagaagtcgc caaccagatc aatgaaaaca agatcggcgt aacggctacc
     1501 gtcatcacca ccgcctccgg tacacaacgc atggtgctca gcggcgacga cacaggcgcc
     1561 agcaacgcct tcagcctgga ctcgacgatg acagcgtccg gcggtcagtc ctcccttggc
     1621 agcttcgata cgagcaccgt cggtctggcc cgcaccgacg cccagaacgc gctgatgaaa
     1681 attgacggca tcgatgtcag ttcgagcacc aacagctttt ccagttcgat taccggattg
     1741 accatcaacg ccaccaaggt cggcagcacg acgctgaccg tggccaccaa caaggacagc
     1801 atcgtctcgt cggttcagtc ctttgtcgat gcattcaatg cggtcgtaga cgacatcaag
     1861 ggcaactcca cctacgatgc gacgaccaag gtcggaggtg cattttcggg agacagcgcg
     1921 gcgcgcggca ttctgtccag cctgaacgag gccaggacca ccgaaccggc cggggtcgcc
     1981 gacagcagct tcaagacgct gtccgatctc ggaatttcca tccagtcgaa cggcaagctg
     2041 tcgctggata gcacagcgct gcaggaagca ctgagtgaga attccggcga tgtcatcaag
     2101 acactgaacg cctacggcaa cgcctttggc gatgttctcg ccgacttcca gggaagcgac
     2161 ggcatcgtca gcaaccgggt caacggtctg acctccgcct cggcaaagtt cgccgacgac
     2221 aaggaagcca tggaattgcg ccttgaactg atcgaaaaac gctaccgcgc gcaattcacc
     2281 gcgctggaca cattggtatc gtcgatgagc acgaccagca gttatctgac gcaaacattc
     2341 gcggccctga gttcatcgag cagctaactc tcgctatact gtcgcaaccc tgcttcaatg
     2401 agccagtaac ccatgtttgc cagttccccc aacgcctaca aacagatcgg gcaggatgcc
     2461 gatattctgg gcgccgatcc gcaccgtctg atcgtcctgc tgtttgacgg cgccgacgcc
     2521 gccttgctca aagccagggc ctgtctcgaa cagaaggata tccgcggccg ctcggacgca
     2581 ctgatcaagg ccatcgacat catttcccat ggtctggcgg acagtctcaa ccttgagcat
     2641 ggcggcgatc tcgccaagaa cctgagcgca ctttacgaat acatggtcag ccgcctgatc
     2701 tatgccaata tccaccagga cgagcgcgcc atcaccgagg tgcagaagct gcttggagaa
     2761 attggcggcg cctggcgcga aatgggcgac aacctgagaa agcaggctgc ggctggagga
     2821 cccaaaccct gaacgaaaaa accccgcaag ggaacaccgc cttcagcgac ctgtcggcag
     2881 cgctggacgc cttgctcaac ctgctccgcc aaggcaactg ggaagccatc gccgaccagc
     2941 agacggctct cttgccggcg ctcgaagccg tcagaaaccc ggccctggct cgggaaaagg
     3001 ccatctctcc cgttcaattg gccagtttgc aggaaaagct tcagcaagcc atcggcgaat
     3061 gccgcgaacg tcaggcgcaa atcgcgcctt tggtagaagc cttcacgaaa aaccgcccgc
     3121 cgcaatctag ctcatgattc cgccggatgt cgccagttcg ctacgctcgg tattgaccga
     3181 gtcgcagaac gcgacccaac agccccagca aacccaacct gtcgcggcga tccagcgcat
     3241 tgccgacgtt ctcggcaacc tcgtgccagg acagcggatc atggccgaaa tccagggcat
     3301 gcaggccaac ggcacctacc gggcgatggt cggccagcgg gaaatcaccc tttccctgcc
     3361 gttttcggcc aaagccggcg acagcctgga actcgaagtc accgaatccg acggcaagct
     3421 ggctctcgcc ttcgtcgcca atcgtggcga acccgccgcc aaaggacagc cggtttcggt
     3481 tgccacttcg ctgagcaata ccggccgcat gatcggcgat ctgctgcagg aactgacggt
     3541 ggacggcaag cgggcaccgg ccgcgccgct caacggcaac caggcgctgg tacagagcat
     3601 gccgaaagat gccgcacaac tggttctcgt actgagacag gccctgacgc aaagcggcat
     3661 gttctacgaa gcccaccagg cgcgctgggc ctctggtcgg ctgccgaccg agcaattgcg
     3721 ccaagagccg caaggccggc tccccgcacc ggccaatccg caaaatcagg gaagcagtcc
     3781 cgcgccgacc gcttcgcccc ctcagcccgc aggcggacca agcccggcgg cgccaggcct
     3841 ccccggcggg gctgcgatga acagcgcccc cacccttccc ggttctaacg gagccgcgcc
     3901 ccctgccccc ggcggcagcg ccccggccgt cagcggcatc ccaactccga cggcgcctcc
     3961 cctcgcc
//
LOCUS       DAOSDX010000158         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8033_length_3965_cov_3.291049, whole genome shotgun sequence.
ACCESSION   DAOSDX010000158 DAOSDX010000000
VERSION     DAOSDX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8033_length_3965_cov_3.291049"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLN02_04585"
     CDS             <1..1220
                     /locus_tag="PLN02_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOI51629.1"
                     /translation="IPRIIPAQEWKALERGMRQRVRALNMFLWDIYHDQEILKAGKIP
                     AEQVLNNAQYRAVMQGVDVPAGIYAHIAGVDVVRAGAGEFYVLEDNLRVPSGVSYMLE
                     DRKMMMRLFPELFARHKVAPVQHYPDMLLEKLRAVAPQGIADPTVVVLTPGAYNSAYF
                     EHSFLAQQMGVELVEGRDLFVKNDTVYMRTTQGPQRVDVIYRRIDDDFMDPLAFRADS
                     ALGVPGILKAYQAGNVTLSNAIGTGVADDKSIYPYVPEMIRFYLGEEPRLNNVPTYMC
                     RKKDDLAYVLDHLAELVVKEVHGAGGYGMLVGPASTKEQIEKFRQLLLAKPDGYIAQP
                     TLALSNCPTFVEEGIAPRHLDLRPFVLSSGKCVNMVPGGLTRVALTKGSLVVNSSQGG
                     GTKDTWVLEDESC"
     gene            1214..2164
                     /locus_tag="PLN02_04590"
     CDS             1214..2164
                     /locus_tag="PLN02_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOI51630.1"
                     /translation="MLSRTADHLFWMSRYIERAENLARLLDVTWQMSLVPQSEEAANQ
                     SWAGIIALNSLEETYAAKYSTVNGENVLRFMVSDPDNFSSIHSCLRLARENAHAVRGT
                     LTTEMWETMNSTWLEAREKSFEQIVNAGIGDYCEWVKMRSSLSRGTTLGTLLQDEAFH
                     FIRLGTLLERADNTARILDVKYHVLRPHGEEEEATDFYQWGALLRSVSGFEVYRKVYR
                     DVITPERVAELLILNSDMPRSLRFCLSGVVKNLELVANSHSGETTRQAGQLYSQLRYG
                     RIDDLLEGGLHEWLTDFMDRIYLIGDGISKDFLVPMAEAA"
     gene            complement(2168..3799)
                     /locus_tag="PLN02_04595"
     CDS             complement(2168..3799)
                     /locus_tag="PLN02_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOI51631.1"
                     /translation="MEGINLAAMLGGVGLFLLGMTMMTDGLKLAVGPSLSGLLHRWTA
                     TRLRGLLFGIVATALVQSSSAITVAAIGFVNAGLLPLGQSLWIFFGANVGTTFTGWLV
                     AVIGFKLKLEAAALPLIGVGVLLRLSGQETRRGAIGNALAGFGVLFLGIGFLQQAFAA
                     GGNAIDLSQLSGHGLWSILAFVLAGAVLTAMMQASAATLAIVLTLAEAGVLPLSEAAG
                     AVIGANIGTTATALLASIGATPNARRAALAHVLFNLLTAVVALLALPLLLSLIARLRE
                     LLELGTQPAVTLALFHSVFNLLGVILMWPLTARLSRFLETRFRTREEEIGRPRYIDRN
                     VGAVPALAVDALHREVSRLGNIVLELARQRSTPADGDGAPDWPALDPFPALARAIVDF
                     VTEVNRHPMPADTARNLAALLRVHRYYDTCHELCAELADIHALPPQIDATLVEEMRAL
                     SAQALHLLAQLDPQPSGKPQLGENALADFELAYHALKARLLEAGASGGLAIDAMEQLL
                     GGFSALRRLVEQAHKAASTLAGFAPLPAEAAAGAS"
BASE COUNT          706 a         1289 c         1341 g          629 t
ORIGIN      
        1 tcattccgcg catcatcccc gcccaggaat ggaaggcgct ggaacgtggg atgcgccagc
       61 gggtgcgggc gctcaacatg ttcctgtggg atatctacca cgaccaggag atcctcaagg
      121 cgggcaagat tccggctgag caggtgctga acaacgcgca gtaccgcgcc gtgatgcagg
      181 gcgtcgatgt gcctgccggc atctacgcgc acatcgccgg cgtcgacgtg gtgcgcgccg
      241 gggctggcga gttctacgtg ctcgaagaca atctgcgggt tccttccggc gtctcctaca
      301 tgctcgagga ccgcaagatg atgatgcggc tcttccccga actcttcgcc cggcacaagg
      361 tggcgccggt ccagcattac ccggacatgc tgctggaaaa actgcgcgcc gtcgcgccgc
      421 agggcatcgc cgacccgacg gtggtggtgc tgacgccggg cgcctacaac agcgcctatt
      481 tcgaacacag cttcctggcc cagcagatgg gtgtcgagct ggtcgagggg cgcgacctct
      541 tcgtcaagaa cgacacggtg tacatgcgca cgacgcaggg gccgcagcgc gtcgatgtga
      601 tctaccggcg gatcgacgac gacttcatgg atccactggc cttccgtgcc gattcggcgc
      661 tcggcgtgcc cggcatcctg aaggcctacc aggccggcaa cgtcaccctg tcgaacgcga
      721 tcggcaccgg cgtcgccgac gacaagtcga tctaccccta cgtgccggag atgatccgct
      781 tctacctcgg cgaggagccg aggttgaaca acgtgccgac ctacatgtgc cgcaagaagg
      841 acgatctcgc ctacgtgctc gaccacctgg ccgaactggt ggtcaaggaa gtgcatggcg
      901 ccggcggcta cggcatgctg gtcggcccgg cctcgaccaa ggagcagatc gagaagttcc
      961 gccagctact gctggccaag ccggacggct acatcgccca gccgacgctg gcgctgtcca
     1021 actgcccgac cttcgtcgag gaaggcatcg cgccgcgcca cctcgatttg cgccccttcg
     1081 tgctgtcctc gggcaagtgc gtgaacatgg tgcccggcgg cctgacccgg gtcgcactga
     1141 ccaagggctc gctcgtcgtc aattcgtcgc aaggcggcgg caccaaggat acctgggttc
     1201 tggaggatga atcatgctga gccgcactgc cgatcacctg ttctggatgt cgcgctacat
     1261 cgagcgcgcc gagaatctcg cccgcctgct cgacgtcacc tggcagatgt cgctggtgcc
     1321 gcaatccgaa gaggccgcca accagagctg ggccgggatc attgccctga acagcctgga
     1381 agaaacctat gccgccaagt attcgacggt caatggcgaa aacgtgctgc gcttcatggt
     1441 cagcgatccc gacaatttct cgtcgatcca tagctgcctg cgcctggcac gcgagaacgc
     1501 ccacgccgta cgcggcacgc tgaccactga gatgtgggaa acgatgaact cgacctggct
     1561 ggaagcccgc gaaaagagct tcgagcagat cgtcaatgcc ggcatcggcg actactgcga
     1621 gtgggtcaag atgcgttcct cgctgtcgcg cgggacgacg ttgggtacct tgttgcagga
     1681 cgaggctttc cacttcatcc gtctcggcac gctgctcgaa cgcgccgaca acacggcgcg
     1741 catcctcgac gtcaagtacc acgtgctgcg cccgcacggc gaggaagagg aagcgaccga
     1801 cttctaccag tggggcgcct tgctccgctc ggtgtccggc ttcgaggtct atcgcaaggt
     1861 ctaccgtgac gtcatcacgc cggagcgagt cgccgaactg ctcatcctca acagcgacat
     1921 gccgcgctcg ctgcgtttct gcctgagcgg tgtggtcaag aacctggaac tggttgccaa
     1981 cagccattcc ggggagacga cgcggcaggc cgggcagctc tactcgcaac tgcgctacgg
     2041 ccgcatcgac gacctgctgg aaggcggcct gcacgagtgg ctgaccgact tcatggaccg
     2101 catctacctg atcggcgacg gcatcagcaa ggacttcctg gtgccgatgg ccgaggccgc
     2161 ctgagcttca gctagcgccc gctgccgcct cggcgggcag cggggcgaag ccggcgaggg
     2221 tgctcgccgc cttgtgcgcc tgctcgacca ggcggcgcag ggcgctgaaa ccgccaagca
     2281 gttgttccat ggcgtcgatc gccaggccgc cgctggcgcc agcttcgagc aagcgggcct
     2341 tgagggcgtg gtaggccagt tcgaaatcgg ccagggcatt ttcgccgagt tgcggtttgc
     2401 ctgacggttg cgggtcgagc tgcgccagca ggtgcagcgc ctgcgcgctg agcgcgcgca
     2461 tttcctcgac caaggtggca tcgatttgcg gcggcagggc atggatgtcg gcaagctcgg
     2521 cgcacagttc gtggcaggta tcgtaatagc gatggacgcg cagcagcgcc gccagattgc
     2581 gtgccgtatc ggccggcatc gggtggcgat tgacctcggt gacgaaatcg acgatggcgc
     2641 gcgccagcgc cgggaaggga tcgagtgccg gccagtcggg ggcgccgtcg ccatctgccg
     2701 gcgtgctgcg ctggcgcgcc agttcgagga cgatgttgcc caggcgcgag acttcgcgat
     2761 gcagcgcatc gaccgccagc gccggcaccg cgccgacatt gcggtcgatg tagcgagggc
     2821 ggccgatttc ttcctcgcgc gtccggaagc gggtttccag gaaacggctg aggcgggcgg
     2881 tgagcggcca catcaggatc acgccgagca ggttgaagac gctgtggaaa agtgccagcg
     2941 tcactgccgg ctgggtgccc agttcgagca gctcgcgcag gcgagcgatc agcgatagca
     3001 gtagcggcag ggcgagcagg gcgacgactg ccgtcagcag gttgaacagc acatgcgcca
     3061 gtgccgcccg gcgggcgttc ggggtggcgc cgatggaggc cagcagggcg gtcgccgtgg
     3121 tgccgatgtt ggcgccgatg accgcgccgg cagcttcgct cagcggcagg acgccggctt
     3181 cggctaaagt caggacgatg gccagggtgg cggccgaggc ctgcatcatc gccgtcagca
     3241 cggcgccggc gaggacgaag gcaaggatcg accagaggcc gtgcccggac aactggctga
     3301 ggtcgatggc gttgccgccg gcggcgaatg cctgttgcag gaagccgatg ccgagaaaga
     3361 ggacgccgaa gccggccagt gcgttgccga tcgcgccgcg ccgcgtttcc tggccggaca
     3421 ggcggagcag gacgccgacg ccgatcaacg gcagcgccgc cgcttccagc ttgagcttga
     3481 agccgatcac ggcgaccagc cagccagtga aggtggtccc cacgttggcg ccgaagaaga
     3541 tccacaatga ttggccgagc ggcagcaggc cggcattgac gaagccgatg gcggcaaccg
     3601 tgatggcgct cgacgactgc accagcgccg tcgccacgat gccgaacagc aggccgcgca
     3661 ggcgggtcgc cgtccaccgg tggagcaggc cggagagcga ggggccgacc gccagcttga
     3721 ggccgtcggt catcatcgtc atgccgagca ggaaaaggcc gacgccgccg agcatcgccg
     3781 ccagattgat tccttccata actgccccta cggtttcctg gcagccagat tgcgttctcc
     3841 ggccgccttc gtcaagcgcc ggtcagcgct cgagcatggc cagcgccaac gcttccgcca
     3901 ccttgatccc gtccacgcca gccgacagga tgccgccggc gtaaccggca ccttcgccgg
     3961 ccggg
//
LOCUS       DAOSDX010000159         3956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8060_length_3956_cov_2.808511, whole genome shotgun sequence.
ACCESSION   DAOSDX010000159 DAOSDX010000000
VERSION     DAOSDX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8060_length_3956_cov_2.808511"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /gene="secA"
                     /locus_tag="PLN02_04600"
     CDS             complement(<1..693)
                     /gene="secA"
                     /locus_tag="PLN02_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585942.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOI51632.1"
                     /translation="MISGLLKKIFGSRNDRLIKQYSQTVKRINALEPQIAALSDAQLQ
                     AKTAEFRQRYGAGEALDDLLPEAFAVVREAGKRVLGMRHFDVQLIGGMVLHYGKIAEM
                     RTGEGKTLVATLPAYLNAISGKGVHVITVNDYLASRDAEWMGRLHRFLGLTVGINLSQ
                     MDHEAKQAAYAADITYGTNNEFGFDYLRDNMAFSLGQKVQRDLFFAIVDEVDSILIDE
                     ARTPLIISGQSEG"
     gene            complement(798..1721)
                     /locus_tag="PLN02_04605"
     CDS             complement(798..1721)
                     /locus_tag="PLN02_04605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOI51633.1"
                     /translation="MQIILVSRHLKSARTITIMPRHVFAAVAVFLLLVLSSSVLFSMM
                     SLHFRLPIVERVLVSIQQQENRETRNYITNNLNMMATRLGELQGRLLQLDSLGNRVSD
                     LAGIKKSPAVAAETKPGQGGPFIPAPLSAGELQQEIERLAAEVDRKADDLSFMEFKLL
                     EKRVEERLMPTTLPVRNALMGSSFGYRNDPFSGLRSRHDGLDFAAPTGTPVLAAADGV
                     VVAASFHPEFGNMVDIDHGEGLLSRYAHLSEIKVTESQIVRRGENIGAVGSTGRSSGP
                     HLHFEVRMLGVPQNPASFLKRGEHFASLKRR"
     gene            1732..2166
                     /locus_tag="PLN02_04610"
     CDS             1732..2166
                     /locus_tag="PLN02_04610"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOI51634.1"
                     /translation="MYKAPQDYLDADAAAGRIMAHARLLMKLSSQFDAVAPAALRHAA
                     HVANYKSGKLVIHADNGAVAAKIRQMHRRLCDELSKKAPECSDIEVKVQPRQNLDRSS
                     TSTIKPISGAAAATLARTTAALPPGPLRSALDSLLQRAARQE"
     gene            complement(2153..2341)
                     /locus_tag="PLN02_04615"
     CDS             complement(2153..2341)
                     /locus_tag="PLN02_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51635.1"
                     /translation="MWLLRLLAVVLVIAVGVGVVLYAFTGRREYLAFSWRLLRYGILF
                     ALLVFALMFVERLAIIPV"
     gene            complement(2349..3263)
                     /gene="lpxC"
                     /locus_tag="PLN02_04620"
     CDS             complement(2349..3263)
                     /gene="lpxC"
                     /locus_tag="PLN02_04620"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOI51636.1"
                     /translation="MLKQRTLKTVIHASGVGLHGGVKVNLTLRPAAPDTGIVFRRVDL
                     PEPVDIPAQAVLVGDTRMCSCLERDGVKVGTIEHLMSALCGLGIDNAWIDLDAPEVPI
                     LDGSAAPFVFLIQSAGIEEQNAAKKFIRVTRPIEVRDGDKWARFEPYDGYRLSFSIVF
                     HHPAIDRSAQQAEIDFAEHSYVREVSRARTFGFMQEVEYLRENGLALGGGLENAIVLD
                     EFRVLNQDGLRYGDEFVKHKILDAVGDLYLLGHPLLAAYASHKGGHALNNQLARALLQ
                     QQEAWELVSFPESASVPQGVTRWLAQTA"
     gene            complement(3352..>3956)
                     /locus_tag="PLN02_04625"
     CDS             complement(3352..>3956)
                     /locus_tag="PLN02_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOI51637.1"
                     /translation="GIAEIITYPGLVNVDFEDVRTVMGEMGRAMMGSAAAAGVDRARI
                     AAEQAVASPLLEGVNLSGARGVLVNITAAKGNLKMKEVNEVMNTVKAFAADDAHIIFG
                     AVYDELMGDALRVTVVATGLGQAAAKRQSFEVINTPMVEAVATGTHDAVSFTSGAAID
                     YNQIADVPAVVRKRNVTVEALANSGVDRYDIPAFLRKQAD"
BASE COUNT          763 a         1237 c         1263 g          693 t
ORIGIN      
        1 accttcggac tgccccgaga tgatcagcgg tgttctggct tcgtcgatca agatcgaatc
       61 cacctcgtcg acgatggcga aaaacaggtc gcgctgcact ttctgcccca agctaaaggc
      121 catgttgtcg cgcaggtagt cgaaaccgaa ttcgttgttg gtgccgtagg tgatgtcggc
      181 ggcgtaggcg gcctgcttgg cctcgtggtc catctgcgac aggttgatgc cgacggtcag
      241 cccgaggaaa cggtgcaggc gccccatcca ttcggcgtcg cgactggcca ggtagtcgtt
      301 gaccgtgatg acgtgcacgc ccttgccgga aatcgcgttg aggtaggcag gcagcgtggc
      361 gaccagggtc ttgccctcgc ccgtgcgcat ttcggcgatc ttgccgtagt gcagcaccat
      421 gccgccgatc aactggacat cgaagtggcg catgccgagc acccgcttgc cggcctcgcg
      481 gacgacggca aaggcttccg gcaacagatc gtcgagcgct tcgccggcgc cgtaccgctg
      541 gcggaactcc gccgtctttg cctgcaactg cgcatcggac aaggccgcga tctgcggctc
      601 gagcgcattg atgcgcttga cggtctgcga gtattgcttg atcagccggt cattgcggct
      661 gccgaaaatc tttttgagta ggccggatat catcgcactg gaatggattc ttgcgccgac
      721 aggcgcaatg gagccgggat tctagcatgc ccgccgggag cgacgggcag cacagcatcg
      781 taccggcaag atcggggtca gcgccgcttc aggctggcaa aatgctcgcc ccgcttgagg
      841 aagctggccg ggttctgggg cacgcccagc atgcgcacct cgaagtgcag atgtggcccg
      901 gaggaacgcc cggtactgcc gacggcaccg atattctcgc cgcggcggac gatctgcgac
      961 tcggtgacct tgatttcgga aagatgggcg tagcgggaca gcaggccctc gccgtggtcg
     1021 atatcgacca tgttgccgaa ctcagggtga aaggaagccg cgacgaccac accatcggcg
     1081 gcggccagca ctggcgtacc ggtcggcgcg gcgaaatcca ggccatcatg gcgcgaacgc
     1141 aggccgctga aaggatcgtt gcggtaaccg aaactggaac ccatcagcgc attcctgacc
     1201 ggcagcgtcg tcggcatcag cctttcctcg acccgcttct caagcaactt gaactccatg
     1261 aagctgaggt cgtcggcttt gcgatcgact tccgcggcca ggcgctcgat ttcctgctgc
     1321 agctcaccgg ccgacagggg cgccgggatg aacggcccac cctgccccgg cttggtttcg
     1381 gcggctacgg ccggactctt tttgatcccg gcgagatcgg agacgcgatt acccagcgaa
     1441 tccagttgca gcaggcgccc ctgcagttca ccgaggcggg tggccatcat gttcaggtta
     1501 ttggtgatgt aattgcgtgt ttcccgattt tcctgctgct ggatcgaaac caggacccgc
     1561 tcgacgatcg gcagccggaa atgcagcgac atcatcgaga acagcaccga cgacgagagg
     1621 acaagaagca ggaaaacagc caccgcggca aacacgtgac ggggcataat ggtgatcgtg
     1681 cgcgccgatt tcagatggcg cgaaaccaag atgatctgca cgtaacctcc tatgtacaaa
     1741 gcgccgcaag actacctcga cgccgatgct gccgcgggcc gcatcatggc ccatgcccga
     1801 ctgctgatga agctgtccag ccaattcgac gcggtcgccc ccgccgccct gcgccatgcc
     1861 gcgcatgtcg ccaactacaa gtcggggaag ctcgttatcc acgccgataa cggcgcggtc
     1921 gccgccaaga tccgccagat gcaccggcgg ctgtgcgacg agttatccaa gaaagcgccc
     1981 gagtgtagcg acatcgaggt aaaagttcaa cctcgacaaa atctcgaccg atcaagcact
     2041 tccacgatca agccgatttc cggcgcggcg gcggcgacac tcgccaggac gacggccgcc
     2101 ctaccgccgg ggccgttgcg cagcgcgctg gacagcttgc tgcagcgcgc tgctagacag
     2161 gaatgatggc caggcgctcg acgaacatca gcgcgaagac gagcaaagcg aacaggatgc
     2221 cgtaacgcag caggcgccag ctgaaggcga gatattcgcg gcgcccggtg aaggcgtaaa
     2281 ggacgacgcc gaccccgacg gcgatgacga gaacgactgc cagcaggcgc agcagccaca
     2341 tcgcggtgtc aggcggtctg cgccagccag cgcgtgacgc cttgcggcac gctcgccgac
     2401 tcgggaaaac tgaccagttc ccaggcttcc tgctgctgca ggagggcgcg cgccaactgg
     2461 ttgttcagcg cgtgaccacc cttgtgcgaa gcgtaggcgg cgagcagcgg gtggccgagc
     2521 aggtagaggt cgccgaccgc gtcgaggatc ttgtgcttga cgaactcgtc gccataacgc
     2581 agcccgtcct ggttgaggac gcggaattcg tcgaggacga tcgcgttctc cagtccgccc
     2641 cccagcgcca gaccgttttc gcgcaggtat tcgacttcct gcatgaaacc gaaggtgcgc
     2701 gcccgcgaca cttcgcggac ataggagtgt tcggcgaaat cgatttccgc ctgctgcgcc
     2761 gaacgatcga tcgccggatg gtgaaagacg atggaaaagc tcaggcgata gccgtcgtag
     2821 ggctcgaagc gcgcccactt gtcgccgtcg cggacttcga tcggccgggt gacgcgaatg
     2881 aatttcttgg cggcattctg ttcctcgatc cccgccgact ggatcaggaa cacgaagggc
     2941 gcggcagagc catcgaggat gggcacttcc ggcgcatcga ggtcgatcca cgcattgtcg
     3001 atgccgaggc cgcagagcgc cgacatcagg tgttcgatgg tgccgacctt gaccccgtca
     3061 cgctccaggc aggaacacat gcgggtgtcg ccgaccaaaa ccgcctgcgc gggaatgtcg
     3121 accggttcgg gcagatcaac gcggcgaaag acgataccgg tatccggagc cgccgggcgc
     3181 agggtcagat tgaccttgac gccaccgtgc agaccgacgc ccgaggcatg aatgaccgtc
     3241 ttcagcgtgc gttgctttag catgttggca agtgattgaa attactgggg aaggcggcgc
     3301 attctaacac aatgcgccgc cgcccttttc agagagagct tttgttacag gtcaatccgc
     3361 ctgcttgcgc aggaaagccg gaatgtcgta acgatcgacc ccgctgttgg ccagcgcctc
     3421 gacggtgacg ttgcgcttcc tgaccacggc cggcacatcg gcaatctggt tgtagtcgat
     3481 cgccgcgccg ctggtgaagg aaacggcatc gtgcgtgccg gtggcgactg cttcgaccat
     3541 cggcgtgttg atcacctcga agctctggcg cttggccgct gcctggccga ggccggtggc
     3601 gacgacggtg acgcgcagcg catcgcccat cagttcatcg tagacggcgc cgaagatgat
     3661 gtgcgcgtcg tcggcggcga aggccttgac ggtgttcatc acttcgttga cttccttcat
     3721 cttcaggttg cccttggcgg cggtgatgtt gaccagcacg ccgcgcgcac cggacaggtt
     3781 cacgccttcc agcagcggcg aagcgacggc ctgttcggca gcgatgcgcg cccggtcgac
     3841 accggccgca gcggccgagc ccatcatggc gcggcccatt tcgcccatca ccgtgcgcac
     3901 gtcttcgaag tcgacgttga ccaggcccgg gtaggtaatg atttcggcga tgccgc
//
LOCUS       DAOSDX010000160         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8096_length_3941_cov_3.311117, whole genome shotgun sequence.
ACCESSION   DAOSDX010000160 DAOSDX010000000
VERSION     DAOSDX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8096_length_3941_cov_3.311117"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PLN02_04630"
     CDS             complement(<1..1195)
                     /locus_tag="PLN02_04630"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOI51638.1"
                     /translation="MSSSIFAAVEMAPRDPILGLNEAFNADARDTKVNLGVGVYFDDN
                     GKIPLLTAVKAAEEVRLKAAPPRGYQPIEGPAAYNTAVQNLLFGKDSDLLAAGRVITA
                     QAIGGTGALKIGADYLKRLNPDAKVYISNPSWENHRALFEAAGFVVEDYAYYDAATRG
                     VNFAGMKADLNKMAPGSVVLLHACCHNPTGADMSDAQWAEVVSICQERGLVPFLDMAY
                     QGFADGIDADAVAIRAFSASGLQFFASSSFSKNFSLYGERVGALSVVTASKEESARVM
                     SQVKRVIRTNYSNPPTHGGALVAAVLASGELRAQWEVELAGMRDRIRAMRSGLVDAIK
                     ATGCAQDFSFVAQQRGMFSYTGLSAAQVERMKEEFGIYAVSTGRICLAALNSKNIDYV
                     CKAIAA"
     gene            1279..3351
                     /gene="uvrB"
                     /locus_tag="PLN02_04635"
     CDS             1279..3351
                     /gene="uvrB"
                     /locus_tag="PLN02_04635"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOI51639.1"
                     /translation="MSESNSSTPVVCFDGSPYRLHQPFPPAGDQPEAIRLLVEGIEDG
                     LSFQTLLGVTGSGKTYTMANVIARTGRPALVLAPNKTLAAQLYSEFKEFFPENAVEYF
                     VSYYDYYQPEAYVPSRDLFIEKDSSINDHIEQMRLSATKSLIERRDVVIVATVSCIYG
                     IGDRDEYHNMILTMRVGDRMDQRDIVKRLTDMQYERNEMDFHRGTFRVRGDVIDIFPA
                     EHAEHAIRVSLFDDEIENLQFFDPLTGQLFHKALRFTVFPASHYVTPRATVVRAIQAI
                     KAELRERIEFFNLHQKLVEAQRIEQRTRFDIEMLDQIGFCKGIENYSRHFSGRQAGEA
                     PPTLIDYLERDAIMFIDESHVTIGQVGGMYKGDRSRKENLVDYGFRLPSALDNRPLQF
                     NEFEAQMRQTIFVSATPADYEQQHAGQVVEQVVRPTGLIDPEVLVRPATTQVDDLLGE
                     INKRIAVAERVLVTTLTKRMAEDLTDFLADNGIKVRYLHSDIDTVERVEIIRDLRLGE
                     FDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHIHGTAILYA
                     DRITQSMQKAIAETERRREKQIAFNEQHGIVPRGVSKKIKDIIDGVYDAESAQTELKA
                     AQQLAVYEAMDEKTVAREIKRLEKQMLECAKNLEFEKAAAARDELFRLRERVFGAAQH
                     DPGGENKQ"
     gene            3348..3824
                     /locus_tag="PLN02_04640"
     CDS             3348..3824
                     /locus_tag="PLN02_04640"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOI51640.1"
                     /translation="MNFRVLLVCMGNICRSPTAEAVLRHYIRNHRLGDKVEVDSAGTH
                     GYHVGEAPDARTQRAARARGYDLSQLRARKVARQDLDYFDLILAMDRTNLDNLQRMAS
                     LEQQGKIRLFMDYAKNFEDDEVPDPYYGLGYGFDLVLDMVEDGAQGIVDELLSRLR"
BASE COUNT          777 a         1179 c         1280 g          705 t
ORIGIN      
        1 ccgcggcgat ggccttgcac acgtagtcga tgttcttgct gttcagtgca gccaggcaga
       61 tgcggccggt cgacacggca tagatgccga attcttcctt catgcgctcg acctgggcgg
      121 cgctcaggcc ggtgtaggag aacatgccgc gttgttgggc aacgaaggag aaatcctggg
      181 cgcagccggt cgccttgatc gcgtcgacca ggccgctgcg catggcgcgg atgcggtcgc
      241 gcatgccggc cagctccact tcccactggg cgcgcagttc gccggaggcg agcaccgcgg
      301 caaccagcgc gccgccatgg gtcggcgggt tggagtagtt ggtgcggatg acgcgcttga
      361 cctgcgacat gacgcgggcc gactcttcct tgctggcggt gacgaccgac agggcgccga
      421 cgcgttcgcc gtacagcgag aagttcttgg agaacgagct ggaggcgaag aactgcagac
      481 cggaggcaga gaaggcgcgg atcgccacgg cgtcggcgtc gatgccgtcg gcaaagccct
      541 ggtaggccat gtcgaggaag ggcaccaggc cgcgttcctg gcagatgctg acgacttcgg
      601 cccactgggc gtcgctcatg tcggcgccgg tcgggttgtg gcagcaggcg tgcagcagga
      661 cgaccgagcc cggggccatc ttgttcaggt cggccttcat gccggcgaag ttcacgccac
      721 gggtggcggc gtcgtagtag gcgtagtcct cgacgacgaa accggcggcc tcgaacaggg
      781 cgcggtggtt ttcccaggac gggttgctga tgtaaacctt ggcgtccggg ttcaggcgct
      841 tcaggtagtc ggcgccgatc ttcagcgcgc cggtaccgcc gatggcctgg gcggtgatga
      901 cgcgaccggc ggcgagcagg tcggaatcct tgccgaagag cagattctgg acggcggtgt
      961 tgtaggcggc cgggccttcg atcggctggt agccgcgcgg cggagctgcc ttcagacgga
     1021 cctcttcggc ggccttgacg gcggtcagca gggggatctt gccgttgtcg tcgaagtaca
     1081 caccgacgcc aaggttgacc ttggtgtcac gcgcatcggc gttgaaagct tcgttaagac
     1141 cgaggatcgg gtcgcgcggg gccatttcca ccgcagcgaa gatcgaagag gacatcagcg
     1201 actccgtgga ataatagaag gatgaaacaa caataacgcg cgatgcctgc atcgcgacga
     1261 caagcgcaga attttatcat gagcgaatcc aacagcagca cgcccgtcgt ctgtttcgac
     1321 ggcagtccgt atcgtttgca ccagccgttt ccgccggccg gcgaccagcc cgaggcgatc
     1381 cgcctgctgg tcgaggggat cgaggatggc ctgtccttcc agaccctgct cggcgtcacc
     1441 ggttcgggca agacctacac catggccaac gtcatcgccc gcaccggtcg cccggcgctg
     1501 gtcctggcac caaacaagac gctggcggcg cagttgtact cggagttcaa ggagttcttt
     1561 ccggagaacg ccgtcgagta cttcgtctcc tactacgact actaccagcc ggaagcctac
     1621 gtaccgtcgc gcgacctgtt catcgagaag gacagttcga tcaacgatca catcgagcag
     1681 atgcgcttga gtgcgacgaa gagtctgatc gagcggcgcg acgtggtgat cgtcgccacc
     1741 gtgtcatgca tctacggcat cggcgaccgc gacgagtatc acaacatgat cctgaccatg
     1801 cgcgtcggcg accgcatgga ccagcgcgac atcgtcaagc gcctgaccga catgcagtac
     1861 gagcgcaacg agatggactt ccaccgcggc accttccgcg tgcgtggcga cgtcatcgac
     1921 atcttcccgg ccgaacacgc cgagcacgcg atccgcgtct cgctgttcga cgacgagatc
     1981 gagaacctgc agttcttcga cccactgacc ggccagcttt tccacaaggc cttgcgcttc
     2041 acagtctttc cggcgtcgca ctacgtgacg ccgcgcgcca cggtggtgcg ggcgatccag
     2101 gcgatcaagg cggaactgcg cgagcgcatc gaattcttca atctgcacca gaagctggtc
     2161 gaggcgcagc ggatcgagca gcgcacccgc ttcgacatcg agatgctcga ccagatcggc
     2221 ttctgcaagg gcatcgaaaa ctactcgcgg catttttccg ggcgccaggc cggggaagcg
     2281 ccgccgacgc tgattgacta cctcgaacgc gacgcgatca tgttcatcga cgaatcgcac
     2341 gtcaccatcg gccaggtcgg cggcatgtac aagggcgacc gctcgcgcaa ggaaaacctg
     2401 gtcgattacg gcttccgcct gccttccgcg ctcgataacc ggccgctgca attcaacgag
     2461 ttcgaggcgc agatgcggca gacgattttc gtctctgcga ccccggccga ctacgagcag
     2521 cagcatgccg gccaggtggt cgagcaggtg gtgcggccga ccggtctgat cgaccccgag
     2581 gtgctggtac gtccggcgac gacgcaggtc gatgatcttc ttggcgaaat caacaagcgg
     2641 attgccgttg ctgagcgggt gctggtgacg acgctgacca agcgcatggc cgaggacctg
     2701 accgattttc tggccgacaa cgggatcaag gtgcgttacc tgcactccga catcgatacc
     2761 gtcgagcgcg ttgaaatcat ccgcgatctg cgcctcggcg agttcgacgt gctggtcggc
     2821 atcaacctgc tgcgcgaagg gctggacatt cccgaggtgt cgctggtggc gattctcgat
     2881 gccgacaagg aaggtttcct gcgctcggaa cgctcgctga tccagacgat agggcgtgcc
     2941 gcgcgtcata ttcatggcac agcgatcctt tacgctgata ggattacgca atccatgcaa
     3001 aaagccatcg ccgagacgga gcggcggcgg gaaaagcaga tcgcattcaa tgagcagcac
     3061 ggcatcgtgc cgcgtggggt gtcgaagaag atcaaggaca tcatcgacgg ggtatacgat
     3121 gccgaatcgg cgcagaccga gctgaaggcg gcgcagcagc ttgcggttta cgaagccatg
     3181 gatgaaaaaa ccgtcgccag ggaaatcaag cggctcgaaa agcagatgct cgagtgcgcg
     3241 aagaacctgg aattcgaaaa agcggcggct gcgcgcgacg aacttttccg gctccgggag
     3301 cgggttttcg gcgccgcgca gcacgaccct ggtggagaaa acaagcaatg aactttcgcg
     3361 tgctgctcgt ctgcatgggc aacatctgcc gttcgccgac cgccgaggcg gtgttgcggc
     3421 attacatccg gaatcatcgt ctgggcgaca aggtcgaggt cgattcggca ggcacccacg
     3481 gctaccacgt cggtgaagct cctgacgcca ggacgcagcg ggcggcaagg gcgcgcggtt
     3541 acgacctgtc gcaattgcgg gcgcgcaagg tggcgcgcca ggatctcgat tatttcgacc
     3601 tgatcctggc catggatcga accaacctgg acaacctgca acggatggcc agcctggaac
     3661 agcagggcaa aatccgcttg ttcatggatt acgcgaagaa tttcgaagat gacgaggtgc
     3721 cggacccgta ctacggtctc ggctacggtt tcgatctggt gctcgacatg gttgaagatg
     3781 gcgcacaggg tatcgtcgac gagttgctaa gccgcttgcg ctgaaacgac gcaaacggaa
     3841 aagggcgtcc gaggacgccc ttttcatcca agccagcctg ttcttagtcc tgcttctggg
     3901 tttcgactat gatcaggggc tcggtttgac tgctttcgac c
//
LOCUS       DAOSDX010000161         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8119_length_3931_cov_2.628483, whole genome shotgun sequence.
ACCESSION   DAOSDX010000161 DAOSDX010000000
VERSION     DAOSDX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8119_length_3931_cov_2.628483"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1291)
                     /locus_tag="PLN02_04645"
     CDS             complement(224..1291)
                     /locus_tag="PLN02_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOI51641.1"
                     /translation="MNRRTCLLAIALGACFAAAQAEILPEGLEVELPEDRVVIYAATD
                     QNIVQPLIDDFEQRHPGVKVEYHDMHSNLLYQRVIDEARRGSKADVVWSSAMDLQVKL
                     VNDGYAQPYRSPETAALPSWAVWKNEAFGTTYEPAVFVYHKPALRRDELPLTHAALIE
                     LLKRNPERFRQRLATYDPALSGVGLMLHTQDAQSNPIIFWNLAQALGAKGIRAYATSA
                     EMLDSIASGETVFGYNVLGSYALTRARSDPRIGVIWPQDYTLVLSRVAFITRDARHPN
                     AARLWLDHLLSRHGQQILARNAGLFAVREDLSADTASSLRSRLGAAFRPILIGSGLLT
                     YLDQAKRRDFLRQWDAAVKPR"
     gene            1385..2053
                     /locus_tag="PLN02_04650"
     CDS             1385..2053
                     /locus_tag="PLN02_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI51642.1"
                     /translation="MRLLLVEDHAELSQWVSKALRHAGYVLDVLDRGDHADHALHTEH
                     YDLVILDLSLPGLDGLEVLRRLRQREKGMPTPVLVLTARGATEERVRGLNTGADDYLT
                     KPFDLSELEARIKALLRRSGNLVPKVRIGQLEFDTTTRLASVGEQALALTPRELAVLE
                     VLLSRSGRPVAREALFEKVFDFDTDARVEAIEIYVSRLRKKLEGSGVVINTLRGLGYL
                     LAEA"
     gene            2084..3502
                     /locus_tag="PLN02_04655"
     CDS             2084..3502
                     /locus_tag="PLN02_04655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOI51643.1"
                     /translation="MPGEARPGPRYSLRRRIVWWLLPPLFVMLAVNAWLSYRGALEAV
                     NRAYDRSLTASIKAVAEQTHSAAGEIQVNIPYSAFEMFEDKAQGRIYYAVIAPSGALL
                     TGYPELAPPAGLSESGEVLIVNSSYRNEEIRLGAMRKRLYDPALQGGDAATIVFAETT
                     GSRTRLARDLFIDSLRRQLLLLGVGALLIVFALGSAFRPLLELRDTIRQRGDEDLTPV
                     PANNVPSEVGPLIDAINHHTERLSAMLAARKRFLADAAHQIRTPLAVLTTQAEYGLRQ
                     SDPEEMLRTFASLLHSIRATRRLANQMLAMSRAEAVNGMMDERAPVDLNALVRDVAME
                     LVPLALNKGIDLAFEAWPQPAVLENGNAPMLREMVANLIDNAIRYSPPESQVTAAVAS
                     WKGKALISVSDQGPGIPEAERENVFHRFYRILGHGDVDGSGLGLAIVREICLAHGGNI
                     SLRDGDDGRGLCVEVELPLAGG"
     gene            complement(3592..>3931)
                     /locus_tag="PLN02_04660"
     CDS             complement(3592..>3931)
                     /locus_tag="PLN02_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOI51644.1"
                     /translation="NWLLVPGVAAISMVGVYAVHGNTFDLVLGTVIGGIGYLLRVMNF
                     PIAPFIVGFVLGDMMEQNLRRALSIGNGEVGVLFGSPIAIGLWVAAIAMLAGPRLMKM
                     REKRAAVAFS"
BASE COUNT          691 a         1339 c         1298 g          603 t
ORIGIN      
        1 ggcctggcgc ccgccggcca gcggctgcag gcgcaggccg gccatgcgcg cgccggccac
       61 cgcgaacagc ggaccgatca tccacggcaa gggcaggctg atggactggg caaacaaggc
      121 ggcaagcagg gccagcccca gggtgggcag gacgaaacgg acaacagggt ggcgcatacg
      181 ggaaaaccgg aagaggcgaa acggggactg gaaacaggcg gaatcagcgg ggtttgacgg
      241 cggcatccca ctggcgcagg aaatcgcggc gcttggcctg gtcgagatag gtgagcagtc
      301 cggaaccgat caggatcggg cggaaggcgg cgcccagccg gctgcgcagg ctgctcgcgg
      361 tgtcggcgga aaggtcctcg cggacggcga acaagccggc gttacgggcc aggatctgct
      421 gcccgtggcg cgacagcagg tggtccagcc acaggcgggc ggcattggga tggcgggcgt
      481 cgcgggtgat gaaggcgacc cggctgagca ccagcgtgta atcctgcggc cagatgacac
      541 ctattctggg gtcgctcctg gcccgggtca gcgcgtagga gccgagcacg ttgtagccga
      601 acacggtttc gccgctggcg atgctgtcga gcatttctgc ggaggtggca taggcgcgga
      661 tgcccttggc gccgagcgcc tgggccaggt tccagaaaat aatcgggttg gattgcgcgt
      721 cctgcgtgtg gagcatcaga ccgacgccgg aaagcgccgg atcgtaggtt gccaggcgct
      781 gcctgaagcg ctccggattg cgtttgagca gttcgatcag cgcggcgtgg gtgagcggca
      841 gctcgtcgcg gcgcaacgcc ggcttgtggt aaacgaatac agccggttcg taggtggtgc
      901 cgaaagcctc gttcttccac accgcccagc tcggcagcgc cgccgtttcc ggcgagcggt
      961 acggctgggc atagccgtcg ttgaccagct tgacctgcag gtccatggcc gaactccaga
     1021 ccacgtcggc cttgctgccc cggcgcgcct cgtcgatcac gcgctggtag agcagattgg
     1081 agtgcatgtc gtggtattcg accttgaccc cggggtggcg ctgctcgaaa tcgtcgatca
     1141 gcggctgcac gatgttctgg tcggtcgccg cgtagatcac gacccggtct tcgggcagtt
     1201 ctacctccag gccctccgga agaatctctg cctgcgccgc ggcaaagcat gcgccaaggg
     1261 cgatggccag caggcatgtt ctccggttca tccgtcttcc tccagtgaca cccgggctgc
     1321 gccatggtgt tttttgttgt tcgagatgat aatccccgtc caagctttcg atttactttc
     1381 acccatgcgt ctgctcctgg tcgaagatca cgccgaactc tcccagtggg tcagcaaggc
     1441 gctgcgccac gccggttacg tgctcgatgt gctcgaccgc ggcgaccacg ccgatcatgc
     1501 gctgcatacc gagcactacg atctggtcat cctcgacctc tcgctgcccg gcctcgacgg
     1561 gctggaagtc ctgcgccgcc tgcgccagcg cgaaaagggc atgcccaccc cggtgctggt
     1621 gctgaccgcg cgcggcgcca ccgaggaacg ggtgcgcgga ctcaacaccg gcgccgacga
     1681 ctacctgacc aaacccttcg acctgagcga gctggaagcg cggatcaagg ccttgctccg
     1741 ccgtagcggc aacctggtgc ccaaggtccg catcggccag ctcgaattcg acaccacgac
     1801 gcgcctggcc agcgtcggcg aacaggccct ggcgctcacc ccgcgcgaac tggcggtgct
     1861 cgaagtgctg ctctcgcgca gcggtcgccc ggtggcgcgc gaagcgctgt tcgagaaggt
     1921 cttcgatttc gacaccgacg cgcgcgtcga ggcgatcgag atctacgtct cgcgcctgcg
     1981 caagaaactc gaaggctcag gggtggtgat caacaccctg cgcggcctcg gctacctgct
     2041 tgccgaggcc tgagcttgtt gtgcctgccg cggtcactcc ccgatgcctg gtgaagcccg
     2101 gcccggtccg cgttacagcc tgcgccgccg gatcgtctgg tggctgctgc cgccgctctt
     2161 cgtcatgctc gcggtcaatg cttggctgtc ctaccgcggc gcgctggaag cggtcaaccg
     2221 cgcctacgac cgttcgctga cggcgtcgat caaggcggtc gccgagcaga cccattcggc
     2281 ggctggcgaa atccaggtca acatccccta ctcggctttc gagatgttcg aggacaaggc
     2341 gcaggggagg atctattacg cggtgatcgc gccctccggc gccctgctca ccggctaccc
     2401 cgaactggcg ccgccggccg ggctgagcga aagcggcgaa gtgctgatcg tcaacagcag
     2461 ctatcgcaac gaggaaatcc gcctcggcgc catgcgcaag cgcctctacg accccgccct
     2521 gcaaggcggc gacgcggcga ccatcgtctt cgccgaaacc accggttcgc gcacccggct
     2581 ggcacgcgac ctgttcatcg acagcctgcg ccggcaactg ctgctcctcg gcgtcggcgc
     2641 cctgctcatc gtcttcgcgc tgggcagcgc cttccggccg ctgctcgagc tgcgcgacac
     2701 catccgccag cgcggcgacg aggacctcac gccggtgccc gccaacaacg tgccgagcga
     2761 agtcggcccg ctgatcgacg ccatcaatca ccacaccgaa cgcctgtcgg cgatgctcgc
     2821 cgcgcgcaag cgcttcctcg ccgacgccgc ccaccagatc cggacgccgc tcgcggtgct
     2881 caccacccag gccgagtacg gcctgcgcca gagcgatccg gaggaaatgc tgcgcacctt
     2941 cgccagcctg ctgcacagca tccgcgccac ccgccggctg gccaaccaga tgctggcgat
     3001 gtcgcgcgcc gaagcggtca acggcatgat ggacgaacgc gcgccggtcg atctcaatgc
     3061 cctggtccgg gacgtcgcca tggaactggt gccgctggcg ctgaacaagg gcatcgacct
     3121 ggctttcgag gcctggccgc agcctgccgt gctcgaaaac ggcaacgcgc cgatgctgcg
     3181 cgaaatggtc gccaacctga tcgacaacgc catccgctac agcccgcccg aatcccaggt
     3241 cacggcggcg gtagcgagct ggaaggggaa agccctgatc agcgtcagcg accaggggcc
     3301 cggcatcccc gaggccgagc gcgagaacgt cttccaccgt ttctaccgca tcctcggcca
     3361 cggcgacgtg gacggcagcg gcctcggcct cgccatcgtg cgcgaaatct gccttgccca
     3421 cggcggcaac atcagcttgc gcgacggcga cgacgggcgc ggcctgtgcg tcgaggtgga
     3481 actgccgctc gccggcggct gagcgccagc gcaaaaaaaa ggggcacccg aaggtgcccc
     3541 gcaaaatcgc cacgcgcagg gactacggcg cagcaaggag atggaaggta gttagctgaa
     3601 cgccactgcg gcgcgctttt cgcgcatctt catcaggcgc ggaccggcca gcatggcaat
     3661 cgcggcaacc cacaggccga tggcgatcgg gctgccgaac aacacgccga cttcaccgtt
     3721 gccgatcgac agggcgcggc gcaggttctg ctccatcatg tcgccgagca cgaagccgac
     3781 gatgaagggg gcaatcggga agttcatcac gcgcagcagg tagccgatac cgccgatgac
     3841 ggtgcccagc accaggtcaa aggtgttgcc atgcaccgcg tacacgccga ccatcgagat
     3901 cgccgcaaca cccggcacga gcagccagtt c
//
LOCUS       DAOSDX010000162         3925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8140_length_3925_cov_2.770026, whole genome shotgun sequence.
ACCESSION   DAOSDX010000162 DAOSDX010000000
VERSION     DAOSDX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8140_length_3925_cov_2.770026"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLN02_04665"
     CDS             complement(<1..317)
                     /locus_tag="PLN02_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI51645.1"
                     /translation="MPYELLIGLRYTRAKRRNHFISFISLISMAGIGLGVAALIVVLS
                     VMNGFQKELRTRILGVASHIQVTGIDGGLDHWQSIAAGVEKHPEVRATAPFVQSQGMF
                     SVDG"
     gene            406..720
                     /locus_tag="PLN02_04670"
     CDS             406..720
                     /locus_tag="PLN02_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOI51646.1"
                     /translation="MIVTNIETVPGKTIVKHLGLVNGSTVRSKHVGRDLMAGLKNIFG
                     GELKGYTELLTESREEAMQRMIDQAKAIGANAVVNVRFSTSSVAAGAAELFAYGTAVV
                     VE"
     gene            725..1171
                     /locus_tag="PLN02_04675"
     CDS             725..1171
                     /locus_tag="PLN02_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOI51647.1"
                     /translation="MFDLILFLVLLLLGFVFGQLAEKRHFKSIIEREKTLRDILVFSE
                     RRIPDEERLEGILVCGSVVVSVDFFKRFVANLRNLVGGRVSSYESLLERARREAVLRM
                     KEEARRKGASGIWNLRLETASIYKGAAQSVGAVEVVAYGTAVRERR"
     gene            1175..2017
                     /locus_tag="PLN02_04680"
     CDS             1175..2017
                     /locus_tag="PLN02_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOI51648.1"
                     /translation="MADYENREVPEGINVSPIHPLKDFALLVGGISAVILIAVFALSL
                     LAGQLARHIPFAQERTLAASLGRWLPAAPEKPGERQRRDYLQALADGLAVNMQLPPEM
                     TLTVHYADTDTVNAMATLGGHIVVFQGLIDTLPSENALAMVLAHEIAHVRHRDPVVAM
                     GRGFAVALALASFSGVGDGVLQQWLGGVGMLPILSFSRAQEEAADAAALQAVVQRYGH
                     VGDAAAFFEQVARRQSMPSGPTLLQTHPDTEKRIAAIRTFAAAHAAQTPSAARPLPAF
                     MRAE"
     gene            complement(1966..2184)
                     /locus_tag="PLN02_04685"
     CDS             complement(1966..2184)
                     /locus_tag="PLN02_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51649.1"
                     /translation="MSDSPISRPSPEQLDRIYAAALIEAAALRRAAIDAALDWLLASP
                     GRMRRRLFSGFLHSARMKAGRGRAADGV"
     gene            2289..3299
                     /locus_tag="PLN02_04690"
     CDS             2289..3299
                     /locus_tag="PLN02_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51650.1"
                     /translation="MPTILVGMQLNLDDADLFIRVAELGTLSAAARERNEPVSQVSRA
                     IARIESALGVRLLHRTTHGLSLTDEGDSFLEHARRMLDIAAEMEGQFTGRLAGPSGLV
                     RISVSPILAQLVIVPALPGLYRRHPGLQVDILADDRVTDLARDGIDLAIRAGVDNAES
                     LVVRQLGEHGRSLYAAPAYLAAFGTPATPADLAGHRLIANSASQSLNRWPFRAFAPSP
                     MPWDSKAGHWLKPSRERTPGAAGGILQVKGHTRADSTAIVLELALAGIGIARLNELLV
                     SPLVRADRLRPVLEEYTERERLPVYAVMLQERHRLPKVRACVDFWLEYFAALRSHRDH
                     ES"
     gene            complement(3296..>3925)
                     /gene="nudC"
                     /locus_tag="PLN02_04695"
     CDS             complement(3296..>3925)
                     /gene="nudC"
                     /locus_tag="PLN02_04695"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOI51651.1"
                     /translation="IGGRPCRALDVADWPALAGGEPVSLRALHGLAGMEVFAFAGRAT
                     QLLDWQANHRHCGSCGTPTARLGGEFAMRCPACGLVVYPRLSPAVMVLVRDGERLLLA
                     RSPHFKEGVYSALAGFVEPGETLEQCAAREVREEVGVEIAGLRYFASQPWPFPNSLMI
                     AFFADYAGGDIVPQPDEIEDAAWFAPDALPLLPDPVSIARRLIDAALAA"
BASE COUNT          703 a         1397 c         1231 g          594 t
ORIGIN      
        1 ccgtcgaccg agaacatccc ctgcgactgg acgaagggcg ccgtcgcccg cacttccgga
       61 tgtttttcga caccggcggc gatcgactgc cagtggtcga gcccgccgtc gatgccggtg
      121 acctggatgt gcgaggcaac gccgaggatg cgcgtacgca gctccttctg gaaaccgttc
      181 atcaccgaga ggacgacgat caacgcagcg acgccgagac cgatgccggc catcgaaatc
      241 agcgaaatga aggaaatgaa gtggttgcga cgcttggcgc gcgtgtagcg caggccgatc
      301 agcaactcgt aaggcaatga catgcgtgtt cccgcaacgc aacaaaaccg ctatggtaca
      361 cgcacccgga aagcccggcc cagcaacctg ttgaggaatt ttgcaatgat cgtcaccaat
      421 atcgaaaccg tccccggcaa aaccatcgtc aagcatcttg gcctggtcaa tggcagcacg
      481 gtgcgctcca agcacgtcgg ccgcgacctg atggccggcc tgaagaacat cttcggcggc
      541 gaactgaagg gctataccga actgctcacc gaatcgcgcg aggaagccat gcagcgcatg
      601 atcgaccagg ccaaggcgat cggcgccaac gccgtggtca atgtccgctt ctcgacctcg
      661 tcggtcgccg ccggcgccgc cgagctgttc gcctacggca ccgccgtcgt cgtggaatga
      721 cgcgatgttc gacctgatcc tattcctcgt cctgctgctg ctcggcttcg tcttcggaca
      781 gctcgccgaa aagcggcatt tcaagtcgat catcgagcgc gagaagacgc tgcgcgacat
      841 cctcgtcttc tccgagcggc gcatcccgga cgaagagcgc ctggaaggca tcctggtctg
      901 cggcagcgtc gtcgtctcgg tcgatttctt caagcgcttc gtcgccaacc tgcgcaatct
      961 ggtcggcggc cgcgtcagtt cctacgaatc gctgctcgaa cgcgcccgcc gcgaagccgt
     1021 cctgcgcatg aaggaagaag cgcggcgcaa gggggcaagc ggcatctgga acctgcgcct
     1081 ggaaaccgcg tcgatttaca agggagcggc gcaaagcgtc ggcgccgtcg aagtcgtcgc
     1141 ctacggcacc gccgtccggg agcgccgctg aagcatggcc gactacgaga accgcgaggt
     1201 gccggaaggc atcaacgtca gcccgatcca cccgctgaag gacttcgcgc tgctcgtcgg
     1261 cggcatcagc gccgtcatcc tgatcgccgt gttcgcgctg tcgctgctcg ccggccagct
     1321 cgcccggcac attccgttcg cccaggaacg gacgctcgcc gccagcctcg gccgctggct
     1381 gccggcggcc ccggaaaaac cgggcgagcg ccagcgccgc gactacctgc aggcgctcgc
     1441 cgacgggctg gcggtgaaca tgcaactgcc gccggaaatg acgctgaccg tgcattacgc
     1501 cgacaccgac acggtcaacg cgatggcgac gctgggcggc cacatcgtcg tcttccaggg
     1561 actgatcgac accctgccca gcgaaaacgc gctggccatg gtgctggccc atgaaatcgc
     1621 ccacgtccgg catcgcgatc cggtcgtcgc catggggcgc ggcttcgccg tcgcgctggc
     1681 gctggccagt ttttccggcg tcggcgacgg cgtgctgcag caatggctgg gcggcgtcgg
     1741 catgctgccc atcctgtcgt tcagccgtgc tcaggaagaa gccgccgatg ccgccgcgct
     1801 gcaggcggta gtgcagcgtt acgggcatgt cggcgatgcc gccgccttct tcgagcaagt
     1861 cgcccgccgg cagtcgatgc cgtccgggcc gacgctgctg cagacgcatc cggacaccga
     1921 aaaacgcatc gcggcaatcc gcaccttcgc cgcagcacac gctgctcaga cgccatcggc
     1981 agcacgcccc ctgcccgcct tcatgcgcgc ggagtgaagg aagccggaaa acaagcgccg
     2041 ccgcatacgc cccggactgg ccagtagcca gtcgagcgcc gcatcgatcg cggcgcgacg
     2101 cagcgccgca gcctcgatca gcgccgccgc gtagatgcgg tcaagctgtt cgggtgacgg
     2161 ccgtgagatt ggggaatcgg acatgattga ctccaggcgt gatggcggtt cgaactctat
     2221 tgtgcaaacc gacacccgga aaccgatgaa atcgcagatt gcttttgcat tatttgcaaa
     2281 agcagttcat gccgaccata cttgtcggca tgcagctcaa tcttgacgat gccgacctgt
     2341 tcatccgcgt tgccgaactc ggcacgctgt cggccgcggc gcgcgaacgc aacgaaccgg
     2401 tcagccaggt atcgcgggcg attgcccgca tcgaatcggc acttggcgtg cgcctgctgc
     2461 accggacgac gcacggcctc tcgctgactg acgagggcga cagcttcctc gaacacgcgc
     2521 gccgcatgct cgacatcgca gccgagatgg aaggccagtt caccggtcgc ctggccggac
     2581 cgagcggcct ggtacgcatc agcgtcagcc cgattctcgc ccagctggtc atcgtccccg
     2641 cgctgcccgg gctttaccgg cgccaccccg gcctgcaggt cgatatcctg gccgacgacc
     2701 gtgtcaccga cctcgcccgc gacggcattg acctcgccat ccgcgccggc gtcgacaacg
     2761 cggaaagcct ggtcgtccgc cagctcggcg aacacggacg cagcctctac gccgctccgg
     2821 cctatctggc agctttcggc acaccggcaa cgcccgctga tctcgccgga catcgcctga
     2881 tcgccaacag cgccagccag tcactcaacc gctggccctt ccgggccttt gcgccgagcc
     2941 cgatgccctg ggacagcaag gccggccact ggctcaagcc gagccgcgag cgaacgcccg
     3001 gcgccgctgg cggaatcctg caggtcaagg gacacacccg agccgacagc accgcgatcg
     3061 tcctcgaact ggccctggcc ggcatcggca tcgcccgcct caacgagctg ctggtctcgc
     3121 cactggtacg cgctgaccgc ctccgtccag ttctggagga gtacacggaa cgcgaacgcc
     3181 tccccgtcta cgcggtgatg ctgcaggaac gccaccgcct gcccaaggtc cgcgcctgcg
     3241 tcgatttctg gctggagtac tttgccgcgc tgcgctcgca ccgcgaccac gagagctagg
     3301 cagccagcgc cgcgtcgatc aaccggcggg cgatgctcac cgggtcgggc agcagcggca
     3361 gcgcgtccgg tgcgaaccag gcggcgtcct cgatctcgtc cggctgggga acgatgtcgc
     3421 cgcccgcgta atcggcgaag aaagcgatca tcagcgagtt cgggaaaggc cacggctgac
     3481 tcgcgaagta gcgcagcccg gcgatctcga ccccgacctc ctcgcgcacc tcgcgcgccg
     3541 cgcattgctc cagcgtttct ccgggttcga caaagccggc cagcgcgctg tagacgcctt
     3601 ccttgaagtg cgggctgcgc gccagcagca gacgctcgcc gtcgcgcacc aggaccatca
     3661 ccgccggcga cagccgcgga tagacgacca gcccgcaggc cgggcagcgc atcgcgaatt
     3721 ccccgcccag ccgcgcggtc ggcgtgccgc aactgccgca atgacggtgg ttggcctgcc
     3781 agtcgagcag ttgcgtcgcc cgtccggcga aggcgaacac ctccatcccg gccaagccgt
     3841 gcaaggcccg cagggaaacc ggttcgccgc cggcgagcgc cggccagtcg gcgacatcga
     3901 gcgcccgaca gggccggccg ccgat
//
LOCUS       DAOSDX010000163         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8204_length_3905_cov_3.728052, whole genome shotgun sequence.
ACCESSION   DAOSDX010000163 DAOSDX010000000
VERSION     DAOSDX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_3905_cov_3.728052"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLN02_04700"
     CDS             complement(<1..752)
                     /locus_tag="PLN02_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOI51652.1"
                     /translation="MTAQIIDGKALAEELRQSFKARVETLTAKGHQPGLVVILVGEDP
                     ASQVYVKNKVNGCLAIGMHSEKIVYDKDVDQQVVLDKIAALNADPSIHGILVQLPLPR
                     HFDEEKVLEAISAEKDVDGFHAENVGALSQGAPRFIPCTPYGVMKMFEKGNVDLSGQE
                     AVVIGRSNIVGKPMAMLLINAGATVTVCHSKTRDLKFHTRRADIVVAAVGKPKFVTGE
                     MIKPGAVVIDVGINRLPDGKLCGDVDFDDCLAV"
     gene            920..2341
                     /locus_tag="PLN02_04705"
     CDS             920..2341
                     /locus_tag="PLN02_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51653.1"
                     /translation="MNAQTPPRLSQTADPTPFWERIPRFFLFPLQPAILGHLFLAAAL
                     PALCFLAPGAISGLLLFFGGSLWAWFSVLRQGSRIAIETSRGRLLPADYDDNTREGLG
                     LLPFSIFALFFVSGLAVGLIQGMFGNTPAMAMNFVVSLLLPAALMVLLHSRSLLAGLN
                     PWRAATLIGSIGKPYLLLCVFLFCLSSAQMFLTWQLYAFGLKPIIADLMAGNIVPESG
                     QTPIDLIGELFARQRMRMSSALFCANLAAMFFTMIGFNMLGYVLYQYHRILGIPLPPT
                     ARGGNPATAAEDGPAAQIAELIAAGEMSRAIDIAYEEQRVHHEDVTAQERYHKLLHLA
                     GRKDRLLPHANRLIPLLLQRQMASRAIAAWRRCHELDADFRLHEPGVELALAEAAHKL
                     RENQLALDLMRAFDKRHPRHPLIPEVYFLSARILCEEFRQDAMAEKIFSAIATRYPQH
                     PCAEKAAEYRRTLARLRGAASPA"
     gene            complement(2368..3408)
                     /locus_tag="PLN02_04710"
     CDS             complement(2368..3408)
                     /locus_tag="PLN02_04710"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOI51654.1"
                     /translation="MAGVVGTRYAFRSDQPTLLTAFSSQFLHADVSHLLGNMLVLVLI
                     GPAVEGLIGSAAFLAIYLLGGVAGAGLWMVAASGSGLIGASGAIAAVMGAFSVLLRWR
                     RIPFFYFIIVYFDIIRAPALLALPIWLLNEAVQLLWLGGGRVAYEAHFGGLLCGGLLA
                     LPLLRRAQQRLLPEEGEARSAVPAATERRERYLAQARKAMRDADFELARQAYGRAAAH
                     AGHDLAVWQECLNVLKLAPASEAFHQASRSFLRMATREAAVQPMLPAVFIDYLQLAQP
                     RPRLDADRLAALAEVFFAQGRMVELERAARLLHALAPADPRCRRLLLAAAGARRNAGD
                     ATVAAALSRLAG"
     gene            complement(3433..3789)
                     /locus_tag="PLN02_04715"
     CDS             complement(3433..3789)
                     /locus_tag="PLN02_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51655.1"
                     /translation="MPYLSRIIPPYLPVGVVLLVVVNLFVFFGLQQGDEARLRHAVEY
                     YSGSVLAEIEPDRYRDHLLGRGDHEAVAKFDRLLKNRRGLAPLLEQMEDDRAFMEELR
                     QGAVVPPSDEIYGSWR"
BASE COUNT          672 a         1369 c         1220 g          644 t
ORIGIN      
        1 acggcgaggc agtcgtcaaa gtccacgtcg ccgcagagct tgccgtccgg gaggcggttg
       61 atgccgacgt cgatgaccac ggcgcccggc ttgatcatct cgccggtgac gaacttcggc
      121 ttgccgaccg cggcgacgac gatgtcggcg cggcgggtgt ggaacttcag gtcgcgcgtc
      181 ttcgaatggc agacggtgac ggtggcgccg gcgttgatca gcagcatcgc catgggctta
      241 cccacgatgt tggagcggcc gatgacgacg gcttcctggc cgctcagatc gacattgccc
      301 ttctcgaaca tcttcatcac gccgtacggg gtgcacggaa tgaagcgcgg cgcgccctgc
      361 gacagcgcgc cgacgttctc ggcgtggaaa ccgtcgacgt ccttctcggc ggagatggct
      421 tcaaggacct tttcttcgtc gaagtgcctg ggcagcggca gttgcacgag gatgccgtgg
      481 atggacggat cggcgttcaa cgcggcgatc ttgtcgagga cgacctgctg atcgacgtcc
      541 ttgtcgtaga cgatcttctc ggaatgcatg ccgatggcca ggcagccatt gaccttgttc
      601 ttcacgtaga cctgggaagc cgggtcttcg ccgaccagaa tgaccaccag gccaggctgg
      661 tgccctttgg ccgtcagggt ttccacgcgt gccttgaagc tctgacgcag ttcctccgcc
      721 aaggccttgc catcgattat ttgtgccgtc atcgtcgtat ccggaaaaca aaaggggaaa
      781 ggcaggctgc ctttccctcg aagtttcagg attttaccag aaccctggag cggcatcctc
      841 attctgcggc cagccgcgcg attcatccgt catcgcacgt gctatgctta cccggcctcc
      901 cgtcgccacc agcccagcca tgaacgcgca aaccccgccg cgcctcagcc agaccgcaga
      961 cccgactccg ttctgggagc gcatcccgcg ctttttcctg tttccgctgc agccagccat
     1021 cctcggccat ctatttctgg ccgcggcgct gccggcgctc tgctttctgg ctcccggcgc
     1081 catcagcggt ttgctgctct tcttcggcgg ctcgctgtgg gcctggttct cagtacttcg
     1141 ccagggcagc cggattgcaa tcgagaccag tcgtggccgc ctgctgccgg cagactacga
     1201 cgacaacaca cgcgaaggac tggggctgct gcccttcagc atcttcgcgc tgttcttcgt
     1261 cagcggactg gcggtcgggc tgattcaggg catgttcggc aacacgccgg cgatggcgat
     1321 gaacttcgtc gtttcgctgc tgctgcccgc ggcgctgatg gtgctgctgc attcgcgcag
     1381 cctgctcgcc ggactcaatc cgtggcgcgc cgcaacgctg atcggcagta ttggcaaacc
     1441 ctacctgctg ctctgcgtct ttctcttctg cctgtccagc gcgcagatgt tcctcacctg
     1501 gcagttgtac gcctttggcc tgaaacccat cattgccgac ctgatggccg gcaacatcgt
     1561 gccggaaagc ggccagaccc ccatcgacct gatcggcgaa ctgtttgccc ggcaacgcat
     1621 gcgcatgtcc tcggcgctgt tctgcgccaa cctggcggcg atgttcttca cgatgatcgg
     1681 cttcaacatg ctgggttacg tcctctacca gtatcaccgc attctcggca tccccctccc
     1741 cccgacggca cgcggcggca atccggcgac ggcggcggaa gatggtccgg cggcgcagat
     1801 tgccgaactg atcgccgccg gcgaaatgag ccgcgccatc gatatcgcct acgaggaaca
     1861 gcgggtgcat cacgaggatg tgacggcaca ggagcgctat cacaagctgc tgcacctggc
     1921 cggccgcaag gaccgcctgc tgccccacgc caaccggctg atccccctgt tgctgcaacg
     1981 ccagatggcc agccgggcaa tcgccgcctg gcgccgctgc cacgaactcg acgccgattt
     2041 ccggctgcac gaaccgggcg tcgaactggc gctcgccgaa gccgcgcaca agctgcgcga
     2101 aaaccagttg gcgcttgatc tgatgcgcgc ctttgacaag cgccacccgc gccaccctct
     2161 gatcccggag gtctatttcc tcagcgcccg catcctgtgc gaggagtttc gccaggacgc
     2221 gatggccgaa aagatcttca gcgccatcgc cacccgctat ccacaacacc cctgcgccga
     2281 gaaagccgcc gaataccggc gcaccctggc ccgcctgcgc ggcgccgcca gcccggcctg
     2341 acccgtcgcc cccagcggcg gctcgcctca gccggcgagg cggctgaggg cggcggcgac
     2401 cgtcgcgtcg ccggcgttcc ggcgcgcccc ggctgccgcc agcaagagtc ggcgacaccg
     2461 cggatcggcc ggcgccagcg cgtgcagcaa gcgtgccgcc cgctccagtt cgaccatccg
     2521 cccctgagca aagaagacct cggccagcgc cgccaggcgg tcggcgtcga ggcgcggccg
     2581 cggctgcgcc agttgcaggt agtcgatgaa cactgccggc aacatgggct gtaccgcggc
     2641 ctcgcgcgtc gccatgcgca ggaagcttcg gctggcctgg tgaaaggcct cgctggccgg
     2701 cgccagcttg aggacgttca ggcattcctg ccagaccgcc agatcgtgcc cggcatgcgc
     2761 cgccgcgcgg ccataggcct ggcgcgccag ctcgaaatcg gcatcgcgca tggccttgcg
     2821 ggcctgcgcc agatagcgct cgcggcgttc cgtcgcggcc ggcaccgccg accgcgcctc
     2881 cccttcttcc ggcaacagac gttgctgagc ccggcgcagc agcggcaggg cgagcagacc
     2941 accgcacagc aagccaccga agtgggcctc atacgccacc cgtccgcccc ccagccagag
     3001 cagttgcacg gcttcgttga gcagccagat cggcagcgcc agcagcgccg gggcgcggat
     3061 gatgtcgaaa tagacgatga tgaaatagaa gaagggaatc cgccgccaac gcagcaggac
     3121 ggaaaacgcg cccatcaccg cagcgatcgc gccggaggcg ccgatcaaac cgctgccgct
     3181 ggcggcgacc atccacaggc cggcgccggc gacgccgcca agaaggtaga tggcaaggaa
     3241 agcggcgctg ccgatcaggc cttccaccgc cggcccgatc agcaccagga ccagcatgtt
     3301 gccgagcaga tgagaaacgt cggcgtgcag gaactggctg gaaaaggcgg tgagcaaggt
     3361 tggctgatcg ctgcggaagg catagcgggt gccgaccacc cccgccatca ggccgtcgaa
     3421 actttgccgc gcctaccgcc agctaccgta aatctcgtcg ctcggcggca ccaccgcccc
     3481 ttgccggagt tcctccatga acgcccgatc atcctccatc tgctcgagaa gcggcgccag
     3541 accgcgccgg ttcttgagca ggcgatcgaa ctttgccacc gcctcatgat cgccgcggcc
     3601 gagcaggtgg tcccggtagc gatcaggctc gatctcggcc agcacgctgc ccgagtaata
     3661 ctcgacggcg tgccggagcc gcgcctcgtc accctgctgc agaccgaaga agacgaacag
     3721 gttgacgacg accagcaaca ccacgccgac cggcaggtag ggcgggatga tgcgggagag
     3781 atagggcatc aacagcatgc cgccgattct tgcagaaagg cgtcaggctg tcgatgtcgc
     3841 cggcatcgcc ggcgacgccg cttcaggcgg cctgcgccgt gccgaccgcc gcccgaccgg
     3901 cggcg
//
LOCUS       DAOSDX010000164         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8243_length_3889_cov_3.340376, whole genome shotgun sequence.
ACCESSION   DAOSDX010000164 DAOSDX010000000
VERSION     DAOSDX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8243_length_3889_cov_3.340376"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLN02_04720"
     CDS             complement(<1..482)
                     /locus_tag="PLN02_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOI51656.1"
                     /translation="MTTRIEHDSFGPIAVPAERLWGAQTQRSLQNFAISGERQPVELI
                     RALAQVKRACALSNQALGRLAADKAGAIVVAADEIIAGRHDEEFPLVVWQTGSGTQTN
                     MNLNEVIANRASELLGGPRGEKRLIHPNDDVNLGQSSNDVYPTAMHVAARLAVVQRLR
                     P"
     gene            complement(696..917)
                     /locus_tag="PLN02_04725"
     CDS             complement(696..917)
                     /locus_tag="PLN02_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51657.1"
                     /translation="MQPGPDFRRNTRLALGVCAGIAAGVSFGDAIGNPAISIGIGAAL
                     GTVLGVVLTRPASSTTDAGLANRNAEETR"
     gene            complement(1020..3086)
                     /locus_tag="PLN02_04730"
     CDS             complement(1020..3086)
                     /locus_tag="PLN02_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOI51658.1"
                     /translation="MTPAIDFRVAHRLPGRIRFVYRADTSFDARGLARAALNLSGVQQ
                     ARVNRAARSLVIDYDPAATDETALSRGLRGLDIAGNAPRLAGVPGKGKGLGEVLGGGA
                     LLAAHPLLPAGLQMPATLVAALPLFAGAVADLRREGINSHVLEALAVAISAARRDYTA
                     ANATIFMLALGDYLEQSIARRSDNLLKKLLHPDTAQVWVEIDGQERQLAAGEVAVGMT
                     IIVAAGTTVPVDGTVLGGEALLNEAAMTGESEPVLRRRGDSVLSGTVVEEGRLRVYAE
                     HVGRNTASARIADYVEQSLAAKSDSQLKAARLADRLVPAVLGLAGSAWLVSGDWQRAA
                     AVLQADYSCALKLATPVAFKSAMYRSGKQGILFKGGDVLERLLAVDTFVFDKTGTLTT
                     GKLAVTDSIAFDPQYSPKDLIDLAASVEEHYFHPLALAVVEAARRHRGQHFDHKEVEF
                     IAAHGVASVVDGKRIVVGSRHFVVEDEGIPVSDHQTVLDKLYRQGKTLLYIGYGGRLL
                     GVIALKDELRPESRATLQRLRDMGVRHLVMLTGDHRRRAEEIARRLGIDECHAELLPQ
                     QKAAIVAGMKADGRRIAFVGDGINDAPALAGAEVGIAMQRGADLARLTSDIVLLEDGI
                     CRVADARAIAQATMRRIDANFRLTVGTNSAILAAAAFGQLSPVAASIAHNGSTIAILL
                     NALRSA"
     gene            complement(3083..3517)
                     /locus_tag="PLN02_04735"
     CDS             complement(3083..3517)
                     /locus_tag="PLN02_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOI51659.1"
                     /translation="MGKKKDKRRQAAQYANWGQPGMAGMNPGMGGGIGQGMYGGMDAG
                     PPPAFDPNALNGLFGAQPGAQPGLLHGLQGMLGSRQSEQFILGALIGAAAVYVLGDEK
                     MRGKLARTAMKLYAGVAGGVEEIKEQMADIRAEVEAEARKSA"
     gene            complement(3498..>3889)
                     /locus_tag="PLN02_04740"
     CDS             complement(3498..>3889)
                     /locus_tag="PLN02_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51660.1"
                     /translation="GEAAAPPQNGDKSRAAPENDHWGRNGMSQRNALVPGGGNEFVRG
                     FVTGGMLGAVQQRGGGLNLDRRTLRLALQGGCALAGGAAAARDLRNGQTALGLGRIAL
                     ATAAVVAIENLLQDQPSKEIGNGQEEG"
BASE COUNT          573 a         1211 c         1419 g          686 t
ORIGIN      
        1 gggcgcagcc gctgcaccac cgccagccgc gcggcgacgt gcatcgcggt cgggtagacg
       61 tcgttgctcg actggccgag attgacgtcg tcgttggggt ggatcaggcg cttctcgccg
      121 cgcgggccgc cgagcagttc gctggcgcgg ttggcgatga cctcgttgag gttcatgttg
      181 gtttgcgtgc cggagccggt ctgccagacg accagtggga attcctcgtc gtggcggccg
      241 gcgatgattt cgtcggcggc gacgacgatg gcgccggcct tgtcggcagc gagccggccg
      301 agggcctggt tgctcagggc gcaggcgcgc ttgacctggg ccagggcgcg gatcagctcc
      361 actggctgac gctcgccgga aatagcgaaa ttctgcagcg aacgctgggt ctgggcgccc
      421 cacaggcgtt cggcggggac cgcgatgggg ccgaagctgt catgttcgat gcgtgttgtc
      481 atgggagtct cgaggcgggg cggcgggaaa aaaaagcctg gctggcagcg agctggcttg
      541 gcctaagtaa gcgagagatg atcaaccggg gcatgcctgc tgaggagtca ggcatgggac
      601 attccggttg cgctcaatat acccggctgg tctttccatg taaataggaa ttgttactat
      661 ttgacgaccg gcggtatggc aagggcgggc tgccgtcagc gggtttcttc ggcgtttctg
      721 ttggccagac cggcgtccgt ggtcgacgac gccggccggg ttagtaccac gcccagcacg
      781 gtgccgaggg cagcgccgat gccgatactg attgccgggt tgccgatcgc gtcaccgaag
      841 ctgacgccgg ctgcgatgcc ggcgcaaacg ccaagtgcca agcgggtgtt gcggcggaag
      901 tcgggacctg gctgcatgcg tcgtatccgg gatggtgaat gggccggcgc gaggccggtc
      961 ggtgttttgg catggtaatg agaatggttc gctaaatcaa gcgcaggcgc aacgcctgtt
     1021 caggcgctgc gcagggcgtt gagcagaatg gcgatggtgc tgccgttgtg ggcgatgctg
     1081 gcggcaaccg ggctcagttg gccaaaggcg gcagcggcca ggatggcgct gttggtgccg
     1141 acagtgagcc ggaaattggc gtcgatgcgg cgcatcgttg cctgggcgat ggcgcgggcg
     1201 tcggcgacgc gacagatgcc gtcctcgagc aggacgatgt ccgaagtcag gcgggcaaga
     1261 tcggcgccgc gttgcatggc gatgccgact tcggcgccag ccagcgccgg ggcgtcgttg
     1321 atgccgtcgc cgacgaaggc gatgcgtcgg ccgtcggcct tcatgccggc gacgatggcg
     1381 gctttctgtt gcggtagcag ttcggcgtgg cattcgtcga tgccgagccg gcgggcgatt
     1441 tcttcggcac gccggcggtg gtcgccggtc agcatgacca gatggcggac gcccatgtcg
     1501 cgcaggcgct gcaaggttgc ccggctttcc ggacgcagtt cgtccttgag cgcgatgacg
     1561 ccgagcaggc ggccgccata gccgatgtag agcagcgtct tgccctgccg gtagagcttg
     1621 tcgagtaccg tctggtggtc gctgaccggg atgccctcgt cctcgacgac gaagtggcgc
     1681 gaaccgacga cgatacgttt gccgtcgacg acgctggcga ccccgtgggc ggcgatgaac
     1741 tcgacttcct tgtggtcgaa atgctggccg cgatgacggc gggcggcctc gacgacggcc
     1801 agtgccagcg ggtggaagta atgttcctcg accgaggcgg cgaggtcgat caggtctttc
     1861 gggctgtatt gcggatcgaa ggcgatcgaa tcggtgaccg ccagcttgcc ggtggtcagc
     1921 gtgccggtct tgtcgaagac gaaggtgtcg accgcgagca gtctttcgag cacgtcgcca
     1981 cccttgaaca ggatgccttg tttgcccgag cggtacatcg ccgatttgaa ggcgaccggc
     2041 gtcgccagct tgagggcgca ggaatagtcg gcctgcagga cggcggcggc gcgttgccag
     2101 tcgccgctga ccagccaggc gctgccggcc aggccaagca cggcgggaac cagtcggtcg
     2161 gcgaggcgcg cggccttgag ctgcgagtcg ctcttggcgg cgagcgattg ttcgacgtag
     2221 tcggcgatgc gcgccgacgc ggtgttgcgg ccgacgtgtt cggcgtagac gcgcagccgc
     2281 ccttcctcga cgacggtgcc ggagagcacg ctatcgccgc gacggcgcag cacgggttca
     2341 ctttcgccgg tcatcgcggc ttcgttgagc agcgcctcgc cgccaagaac ggtgccatcg
     2401 acgggaacgg tggtgccggc ggcgacgatg atggtcatgc cgaccgcgac ctcgccggcc
     2461 gcgagctgtc tttcctgacc gtcgatttcg acccagacct gagcggtgtc cggatgcaga
     2521 agtttcttca gcaggttgtc cgagcggcgg gcgatcgact gctcgaggta gtcgccgagc
     2581 gccagcatga agatcgtcgc gttggccgcc gtgtaatcgc ggcgggcggc cgaaatggcg
     2641 actgccagtg cctccaggac gtgcgaattg atgccttccc ggcgcaggtc ggcgacggcg
     2701 ccggcaaaca acggcagcgc tgcaaccaag gtggccggca tctgcaggcc ggccggcagc
     2761 agcgggtggg cggcgagcag ggcgccgcca cccaagactt cgcccagccc cttgcccttg
     2821 ccgggaacgc ctgccagacg cggcgcgttg ccggcgatgt ccagcccgcg tagcccccgg
     2881 ctcagggcgg tttcatcggt cgccgccggg tcgtaatcga tgaccagcga gcgggccgcc
     2941 cgattgaccc gcgcctgctg cacgccggac aggttcagcg ccgcgcgggc cagtccgcgg
     3001 gcgtcgaagc tcgtgtcggc gcgatagacg aagcggattc tgccgggcag gcgatgcgcc
     3061 acccggaagt cgatggccgg ggtcatgcgc tcttgcgggc ctcggcttcg acttcggccc
     3121 ggatgtcggc catctgttcc ttgatttcct cgacgccgcc agcgacaccg gcatagagct
     3181 tcatcgccgt cctggccagt ttgccgcgca tcttctcgtc gcccaggaca tataccgcgg
     3241 ccgcgccgat cagtgcgccg aggatgaact gttcgctctg gcgtgaaccg agcatgccct
     3301 gcaggccgtg cagcagtccg ggttgcgcgc cgggctgggc gccgaacagg ccgttcaacg
     3361 cattcggatc aaatgccggt ggcgggccgg cgtccatgcc gccgtacatg ccctggccga
     3421 taccgccacc catgcccggg ttcatcccag ccatgccggg ctggccccag ttggcgtatt
     3481 gcgcggcctg acgccgttta tccttcttct tgcccatttc cgatctcctt ggatggctga
     3541 tcctgtaata aattttcgat ggcgacgaca gcggcggtgg cgagcgcgat gcgccccagg
     3601 ccaagggcgg tctgtccgtt ccgcaagtcg cgggcggcgg ctgcgccgcc ggcaagcgcg
     3661 cagccgccct gaagggcgag acgcagggtg cgccggtcaa ggttcaggcc gccgccgcgc
     3721 tgctgcaccg cgccgagcat gccgccggtg acgaagccgc ggacgaactc gttgccgccg
     3781 ccgggcacga gcgcattgcg ttggctcatg ccgtttcgtc cccagtggtc gttttccggc
     3841 gcggcgcggc ttttgtcgcc gttttgcggc ggcgcggcgg cttcgcctc
//
LOCUS       DAOSDX010000165         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8246_length_3886_cov_3.412164, whole genome shotgun sequence.
ACCESSION   DAOSDX010000165 DAOSDX010000000
VERSION     DAOSDX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8246_length_3886_cov_3.412164"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLN02_04745"
     CDS             complement(<1..347)
                     /locus_tag="PLN02_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI51661.1"
                     /translation="MRPADILRFARDAAAGYPLRTTLSVLAMAIGVAAVVLLTALGDG
                     ARRYVVGQFSSLGTNLVIVLPGRTGTGGFNPATAITSTPRDLTIADAQALLRAPAVRR
                     VGPLAVGTSEISAG"
     gene            complement(334..1023)
                     /locus_tag="PLN02_04750"
     CDS             complement(334..1023)
                     /locus_tag="PLN02_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI51662.1"
                     /translation="MALIELAGIERRFQLGDTTVKALVGIDLAIDAGEYVAVMGPSGS
                     GKSTLLNLLGLLDRPDIGHYRLEGRDVTTLSADEQAKVRSTRIGFVFQSFHLVPRLTA
                     AENIALPMMLAGIAPAERARRVTAALKDFGLDQRADHKPDQLSGGQRQRVAIARATIM
                     QPPLILADEPTGNLDRHTGEDVVNLLEALNARGVTLIVVTHDPGMGERARRRLVMEDG
                     RLQSDSAGAPG"
     gene            complement(1024..2160)
                     /locus_tag="PLN02_04755"
     CDS             complement(1024..2160)
                     /locus_tag="PLN02_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI51663.1"
                     /translation="MRRLAIALVVILLLGGAVFALSRPKPLAVIVHEVGRGKVEATLA
                     NTRAGTVEACQRTKLSTIMGGRIEYLGVKEGDRVEKGQLLMKLWNDDQQAQANLAQTQ
                     IALATRRSDEACIAATNAESEAKRQADLRARGFVSPSKEEAARTEAAVRRAACNTSKA
                     DVAQAEAKLKATRVEQGRVSLYAPFAGTVAKIVGELGEYSTPSPPGVPTPPAIDLIDD
                     SCLYVKAPMDEVDAPKIAAGQPVRITLDALPGQSLPGKVRRVAPYVSAVEKQARTVDI
                     EVDFAKPEQAARLLVGYSADVEIILAGRDKVLRIPTAALQEGGQVLVLKDGALEARPV
                     KTGLSNWEYAEVLDGLQEGEQIVTSLERAGVKAGVKAVADSAAK"
     gene            complement(2240..2548)
                     /locus_tag="PLN02_04760"
     CDS             complement(2240..2548)
                     /locus_tag="PLN02_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51664.1"
                     /translation="MKSGIAATLLLALLAAPVLAQVSDGKREQFRERAGAKLAERFRQ
                     ADANGDGRLTRDEAKGKMPRVYENFTRIDSTGKGYVSEDELRRFAAEQLAERRGAAGS
                     "
     gene            complement(2559..3080)
                     /locus_tag="PLN02_04765"
     CDS             complement(2559..3080)
                     /locus_tag="PLN02_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51665.1"
                     /translation="MHHASRPLFALACTTGALLAALAATPAEAGGRLAVRGHDGAAVG
                     AVRHGPNGGGFARGRAISGDGQGNGRLVSGGAFRGPNGGSGARAGETTRAADGSLNHR
                     SGFQASNANGSVSSQGSATRSADGEITQSRTTTATGAAGNTYNGSTSYSKETGVVHSA
                     SCTDAAGAAIPCR"
     gene            3274..>3886
                     /locus_tag="PLN02_04770"
     CDS             3274..>3886
                     /locus_tag="PLN02_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI51666.1"
                     /translation="MSAAPENISLLILDDEAELRALLQRYLGEQGLRVRAIGEPAQLD
                     RLLTRERFDLLILDLMLPGEDGLSICRRLRAAGETIPIIMLTAKGDPVDRIIGLEMGA
                     DDYLAKPFTARELLARIQALLRRQRMTLGGTARPADEVLRFGRCELNAATRRLLRDGQ
                     PVPLGSTEFALLRALVANAGRPLGRERLLELAHGRDHVALDRSI"
BASE COUNT          499 a         1324 c         1431 g          632 t
ORIGIN      
        1 ccggcgctga tttccgaggt gccgacggcg agcgggccga cccggcgcac ggccggcgcg
       61 cgcagcagcg cctgggcgtc ggcgatggtc aggtcgcgcg gcgtgctggt gatcgcggtg
      121 gccgggttga agccgccggt gccggtacgg ccgggcagga cgatgaccag gttggtgccg
      181 agcgacgaga actggccgac cacgtagcgc cgcgcgccgt cgccgagcgc ggtgagcagc
      241 acgacggcgg cgacgccgat ggccatggcc agcaccgaca gcgtcgtgcg cagcggatag
      301 ccggcggccg cgtcgcgcgc gaagcgcagg atgtcagccg ggcgcaccgg cgctgtccga
      361 ttgcaggcgg ccgtcttcca tcaccagccg gcggcgggcg cgctcgccca tgcccgggtc
      421 gtgggtgacg acgatcagcg tgacgccgcg cgcgttgagc gcttcgagca gattgaccac
      481 gtcctcgccg gtgtggcggt cgaggttgcc ggtcggctcg tcggcgagga tcagcggtgg
      541 ctgcatgatc gtcgcgcggg cgatggcgac gcgctggcgc tggccgccgg agagctggtc
      601 cggcttgtgg tcggcgcgct ggtcgaggcc gaaatccttg agtgccgccg tcacccgtcg
      661 cgcccgctcg gccggcgcga tgccggccag catcatcggc agcgcgatgt tctcggcggc
      721 ggtcagccgc ggcaccaggt gaaagctctg gaaaacgaag ccgatgcgcg tgctcctcac
      781 cttcgcctgc tcgtcggcgc tcagtgtcgt cacgtcgcgg ccttccagcc ggtagtggcc
      841 gatgtcgggg cggtcgagca ggccgagcag gttgagcagc gtcgatttgc cggaaccgga
      901 cgggcccatc acggcgacgt actcgccggc gtcgatagcg aggtcgatgc cgaccagcgc
      961 cttgaccgtg gtgtcgccga gctggaagcg gcgctcgatg ccggcgagtt cgatcagcgc
     1021 catctatttc gccgcgctgt cggcaaccgc cttgaccccg gccttgacgc cggcccgctc
     1081 gagcgaggtg acgatctgct cgccttcctg caggccgtcg agtacctcgg cgtattccca
     1141 gttggacagg ccggtcttca ccggccgcgc ttcgagtgcg ccgtccttca gcaccagcac
     1201 ctggccgccc tcctgcagcg cggcggtcgg gatgcgcagc accttgtcgc ggccggcgag
     1261 gatgatctcg acgtcggcgc tatagccgac cagcagcctg gcggcctgtt ccggtttcgc
     1321 gaaatcgacc tcgatgtcga ccgttcgcgc ctgcttctcg accgccgaga cgtagggcgc
     1381 gacgcgccgt accttgcccg gtagcgactg cccgggcagc gcgtcgaggg tgatgcgcac
     1441 cggctggccg gcggcgatct tcggcgcgtc gacctcgtcc atcggcgcct tgacgtagag
     1501 gcaggaatcg tcgatcaggt cgattgccgg cggcgtcggc acgcccggcg gcgacggcgt
     1561 cgaatattcg cccagttcgc cgacgatctt ggcgacggtg ccggcaaacg gcgcgtagag
     1621 cgaaacgcgg ccctgctcga cgcgcgtcgc cttcagtttc gcctcggcct gggcgacgtc
     1681 ggccttgctg gtgttgcagg cggcgcggcg caccgccgcc tcggtgcggg cggcctcttc
     1741 cttgctcggc gagacgaagc cgcgggcgcg caggtcggcc tggcgcttcg cctcgctctc
     1801 ggcgttggtg gcggcgatgc aggcctcgtc gctgcgccgc gtggcgagcg cgatctgcgt
     1861 ctgcgccaga ttggcctgcg cctgctggtc gtcgttccac agcttcatca gcaactggcc
     1921 cttctcgacc cggtcgcctt ccttgacccc gagatattcg atgcgcccgc ccatgatcgt
     1981 cgacagcttg gtgcgctggc aggcctcgac cgtgccggcg cgggtgttgg cgagcgtcgc
     2041 ctcgaccttg ccgcggccga cctcgtggac gatgacggca agcggcttgg gccggctcag
     2101 cgcgaaaacg gcgccgccga gcaggaggat gacgacgagg gcgatggcga gacggcgcat
     2161 ggcggcgatc cgagggcggt ggcagatggc gatggtcccg tgaacccgcc ccaagatcaa
     2221 ggcaaggccc gggtggaatt caggaacccg cagcgccccg tcgttcggca agctgttcgg
     2281 cggcgaagcg gcgcagttcg tcctcgctga cgtagccctt gccggtgctg tcgatgcgcg
     2341 tgaagttttc gtacacgcgg ggcatcttgc ctttcgcctc gtcgcgcgtc aggcggccgt
     2401 cgccattggc atcggcctgc cggaaacgtt cggcgagctt ggcgccggcc cgttcgcgga
     2461 actgttcgcg cttgccatca ctgacttgcg ccaggacggg ggcggcgagc agggcgagca
     2521 gcagggtggc agcgattccg gatttcatgg ccttctcctc agcggcaggg aatggcggcg
     2581 ccggcagcgt cggtgcaact ggcgctatgg acgacgccgg tttccttgct gtagctggtg
     2641 ctgccgttgt aggtattgcc ggcggcgccg gttgccgtcg tcgtccgcga ctgggtgatc
     2701 tcgccgtcgg cgctgcgcgt cgcgctgcct tggctgctga cgctgccgtt ggcattgctc
     2761 gcctggaagc cgctgcgatg gttcaggctg ccgtcggcgg cgcgcgtcgt ctcgccggcg
     2821 cgggcgccgc tgccgccgtt cgggccgcgg aaggcgccgc cgctgaccag gcgaccgttg
     2881 ccctggccgt cgccgctgat ggcgcgaccg cgggcgaagc cgccgccatt agggccgtgg
     2941 cggacggcac cgacggcggc gccgtcatgg ccgcggacgg cgaggcggcc gccggcttcc
     3001 gcaggggttg cggcgagcgc ggcaagcagg gcgccggtgg tgcaggcgag ggcgaacagg
     3061 gggcgtgaag catggtgcat gatgatctcc ttggctgatg gggccgcggg cttgcggcat
     3121 ggctgtcatt ctgggcagca gtcgtttgcc gccgatgtcg acgaaggggg tgtttgtcac
     3181 agttcgtgtc ggccgccgcc cgttgacatg gtttgacatc gataggcggg gaggtgggct
     3241 gacggcgcgg cagcgggctg cgacaatgcc gccatgtcag ccgccccgga aaacatttcg
     3301 ctcctcatcc tcgacgacga agccgagctg cgcgccctgc tgcagcgcta tctcggcgag
     3361 caggggctgc gcgtgcgcgc catcggcgaa ccggcgcagc ttgaccgcct gctgacgcgc
     3421 gaacgtttcg acctcctcat cctcgacctc atgctgcccg gcgaggacgg tctcagcatc
     3481 tgccggcgcc tgcgcgcggc tggcgagacg attccgatca tcatgctgac cgcgaaaggc
     3541 gatccggtgg accgcatcat cggcctcgaa atgggcgccg acgactatct cgccaagccg
     3601 ttcaccgcgc gcgaactgct cgcccgcatc caggccctgc tgcgccgcca gcgcatgacg
     3661 ctgggcggca ccgcgcgccc ggccgacgaa gtgctgcgtt tcggccgctg cgaactgaac
     3721 gcggcaacgc gccgcctgct gcgcgacggc cagccggtgc ccctgggcag caccgagttc
     3781 gccttgctcc gcgccctggt tgccaacgcc ggacgtccgc tggggcgcga acgcctgctc
     3841 gaactggcgc atggccgcga ccatgtcgcg ctcgaccgca gcatcg
//
LOCUS       DAOSDX010000166         3882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8253_length_3882_cov_2.603606, whole genome shotgun sequence.
ACCESSION   DAOSDX010000166 DAOSDX010000000
VERSION     DAOSDX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8253_length_3882_cov_2.603606"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="PLN02_04775"
     CDS             <1..97
                     /locus_tag="PLN02_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOI51667.1"
                     /translation="PEVRDIFAFRFEDFRLEGYDPHPHIAAPVAV"
     gene            100..1692
                     /gene="pgi"
                     /locus_tag="PLN02_04780"
     CDS             100..1692
                     /gene="pgi"
                     /locus_tag="PLN02_04780"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOI51668.1"
                     /translation="MSRLTATPAWQALARHAGEMRQHHLRDLFAADQQRFAELSLQHN
                     GLLLDFSKQRLSTATLALLRELAEQTNWRQWRERMRNGEAINHTEQRAVGHHWLRAGV
                     NAPEDVRASLSRLAAFCESVHSGEWRGYTGERISDVVHLGIGGSDLGPRMAVQALAAS
                     QHPDLRVHFVANLDGADLASQLSRLDPRRTLFIVASKTFTTLETTVNASTARAWLLHA
                     AGNEAAVARHFVAASSNQAAAAAFGIAPENVFPFAEWVGGRFSLWSSIGLPVALAIGF
                     RNFEQLLAGAHDLDEHFFTAPAASNLPLTLALIGIWNRNFLGAHSHAIFPYSHSLRLL
                     PSHLQQLEMESNGKRVDRDRFAVDYQTNPIVWGVAGTNGQHSFFQLLHQGSELVASDF
                     IAVARPDFPLPGHHSGLLASCLAQAAALAFGQTAEEARAAGIDESLVAYRSFPGNQPS
                     STLILPALTPFSLGQLIALYEHKVFCQGVLWHLNSFDQWGVELGKQLAGRLLPVIRGE
                     GVAPELDASTRGLIDYLRKVRY"
     gene            1694..2170
                     /locus_tag="PLN02_04785"
     CDS             1694..2170
                     /locus_tag="PLN02_04785"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOI51669.1"
                     /translation="MPEIILIVAVARNRVIGKDNRLLWNIPEDMAHFKRLTAGHAVLM
                     GRKTWESLPERFRPLPGRRNIVISRQRDYNAPGAELAHALTDGVALAADAEKLFVIGG
                     AQIYEQAMALADRLEITEVELAPEGDAWFPEISAADWQESARSAGPGCSFVSYRRR"
     gene            complement(2189..>3882)
                     /locus_tag="PLN02_04790"
     CDS             complement(2189..>3882)
                     /locus_tag="PLN02_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HOI51670.1"
                     /translation="PVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPVAASERNAA
                     RIFNCDHLYFVTNGTSTSNKIVWNSTVAPGDIVVVDRNCHKSILHSIIMTGAIPVFLM
                     PTRNNFGIIGPIPKSEFAWENIQKKIDANPFIEDKTAKPRVLTITQSTYDGVLYNVED
                     IKEMLDGKIDTLHFDEAWLPHAAFHDFYGDYHAIGADRQRCKESMIFATQSTHKLLAG
                     LSQASQILVQDCEERHLDRDAFNEAYLMHTSTSPQYSIIASCDVAAAMMEAPGGTALV
                     EESIAEALDFRRAMRKVDEEWGTGWWFKVWGPDDLSEEGIEERDAWMLKPGHRWHGFG
                     KLAEGFNMLDPIKATIITPGLDVDGDFGERGIPAAIVTKYLAEHGVIVEKCGLYSFFI
                     MFTIGITKGRWNTLVTALQQFKDDYDKNQPLWKVLPEFVQKHPRYERMGLKDLCKQIH
                     AVYKANDVARLTTEMYLSDMVPAMRPADAWARMAHREIERVRLDELEDRITSVLLTPY
                     PPGIPLLIPGERFNATIVRYLKFARDFNARFPGFETDIHGLVKREDGEYYVDCVR"
BASE COUNT          645 a         1283 c         1241 g          713 t
ORIGIN      
        1 tcccgaggtg cgggacatct tcgccttccg cttcgaggat ttccgcctcg aaggctacga
       61 cccgcacccg cacatcgcgg cgccggtcgc cgtctgagca tgagccgact gaccgcgact
      121 ccggcctggc aggccctggc gcgacacgcc ggggaaatgc gccagcatca tctgcgcgac
      181 ctgtttgccg ccgaccagca gcgcttcgcc gagctttcgc tgcagcacaa cgggctgctg
      241 ctcgattttt ccaaacagcg cctgagcacc gccacactgg cgttgctgcg cgaactcgcc
      301 gaacagacga actggcgaca gtggcgggaa cggatgcgca acggcgaggc gatcaaccat
      361 accgagcagc gcgccgtcgg ccatcactgg ctgcgtgcag gcgtcaacgc accggaagat
      421 gtacgcgcct ccctcagccg tctggcggct ttctgtgaga gcgtccacag tggtgaatgg
      481 cggggttaca ccggcgagcg gatcagcgac gtcgttcatc tcggcattgg tggttccgac
      541 ctcggaccac gcatggccgt ccaggcgctg gcggccagcc agcaccccga cctgcgcgtc
      601 catttcgtcg ccaacctcga tggcgccgac ctggccagcc agctctcccg actcgatccg
      661 cgccgcaccc tgttcatcgt cgccagcaag accttcacca cgctggagac cacggtcaat
      721 gccagcaccg cccgcgcctg gctgctgcac gcggccggga acgaagcagc ggttgcccgc
      781 catttcgtcg ccgcttccag caatcaggcg gcggcggccg ccttcggcat tgccccggaa
      841 aacgtcttcc cgttcgccga atgggtcggc ggtcgcttct ctttgtggtc gtcgatcggc
      901 ctgccggttg cattggccat cggcttccgt aatttcgagc aattgctggc cggcgcccac
      961 gatctcgacg agcacttctt taccgccccc gccgccagca acctgccgct gaccctggcc
     1021 ctgatcggca tctggaaccg caacttcctc ggcgcccaca gtcacgccat ctttccctac
     1081 agccactcgc tgcgcctctt gccgagccat cttcagcagt tggaaatgga aagcaacggc
     1141 aagcgcgtcg accgcgaccg cttcgccgtc gattaccaga ccaacccgat cgtctggggc
     1201 gtcgccggaa ccaacggtca gcactccttt ttccagttgc tgcaccaggg tagcgaactg
     1261 gtggccagcg acttcattgc cgtggcccgc ccggatttcc ccctgcccgg ccaccatagc
     1321 ggcctgctcg ccagttgcct ggcccaggcg gcggcgctgg ctttcggcca gacagcggag
     1381 gaagcacgcg ctgccggcat cgatgagtct ctggtcgcct atcgcagttt ccccggcaac
     1441 cagccatcga gcaccctgat cctgccggcg ctgacgccgt tcagtctggg ccagctaatc
     1501 gccctgtatg aacacaaggt cttctgccag ggcgtattgt ggcacctcaa ttcctttgac
     1561 cagtggggcg tcgaactcgg caaacaactg gccggccgcc tcctgccggt gattcgcggc
     1621 gaaggcgtcg cacctgaact cgatgcctcg acccgcggcc tgatcgacta cctccgcaaa
     1681 gtgcgctact gacatgcccg aaatcatcct gatcgtcgcc gtcgctcgca accgcgtgat
     1741 cggcaaggac aaccggctgc tctggaacat tcccgaggac atggcgcatt tcaagcgcct
     1801 gaccgccggc catgccgtgc tgatgggacg caagacctgg gagtcgctgc ccgagcgctt
     1861 ccggccgctg cccggccgac gcaacatcgt gatcagccga caacgggatt acaacgcgcc
     1921 aggcgccgaa ctggcccacg cgctgaccga cggcgtcgcc ctggcggcgg acgcggagaa
     1981 gctgttcgtc atcggcggcg cgcagatcta cgaacaggcg atggcgctcg ccgaccgcct
     2041 ggaaatcacc gaagtcgagc tggcgccgga gggcgacgcc tggttcccgg aaatctccgc
     2101 tgccgactgg caggaaagcg cacgcagcgc cggtcccggc tgcagcttcg tcagctaccg
     2161 gcggcgctga ggccgccggc aagcggggtc agcgcacgca atcgacgtag tactcgccgt
     2221 cctcgcgctt gaccaggccg tggatgtcgg tctcgaagcc ggggaagcgg gcgttgaagt
     2281 cgcgcgcgaa cttcaggtaa cggacgatgg tggcgttgaa gcgctcaccc ggaatcagta
     2341 gcgggatgcc cggcgggtag ggcgtcagca gcaccgaggt gatgcggtct tcgagttcgt
     2401 cgaggcggac acgctcgatc tcgcggtgcg ccatgcgcgc ccaggcgtcg gccggacgca
     2461 tcgccggcac catgtccgag aggtacatct cggtggtcag gcgggcgacg tcgtttgcct
     2521 tgtacaccgc gtggatctgc ttgcacaggt ccttgaggcc catgcgctcg tagcgcgggt
     2581 gcttctgcac gaactcgggc agcaccttcc acagcggctg gttcttgtcg taatcgtcct
     2641 tgaactgctg cagcgcggtg accagcgtgt tccagcggcc cttggtgatg ccgatggtga
     2701 acatgatgaa gaagctgtag aggccgcact tctcgacgat gaccccgtgt tcggcgaggt
     2761 acttggtgac gatcgctgcc gggatgccgc gttcgccgaa gtcgccgtcg acgtcgaggc
     2821 cgggggtgat gatcgtcgcc ttgatcgggt cgagcatgtt gaagccttcg gcgagtttgc
     2881 caaagccgtg ccaacggtgg ccaggcttca acatccaggc atcgcgctcc tcgatgcctt
     2941 cctcggacag gtcgtccggc ccccagacct tgaaccacca gccggtgccc cactcctcat
     3001 cgaccttgcg catggcacgg cggaagtcga gcgcttcggc gatcgattcc tcgaccagcg
     3061 cggtgccgcc cggcgcttcc atcatcgccg ccgcgacgtc gcacgaggcg atgatcgagt
     3121 actgtggcga ggtcgaggta tgcatcaggt aggcctcgtt gaaggcatcg cggtcgaggt
     3181 ggcgttcctc gcagtcctgg acgaggatct gcgaggcttg cgagaggccg gcgagcagct
     3241 tgtgcgtcga ttgcgtggcg aagatcatcg attccttgca gcgctggcga tccgcgccga
     3301 tggcgtgata gtcgccgtag aaatcgtgga aggcggcgtg cggcagccag gcttcgtcga
     3361 agtgcagggt gtcgatcttg ccgtcgagca tctccttgat gtcctcgacg ttgtagagca
     3421 cgccgtcgta ggtcgactgg gtgatcgtca gcacacgcgg cttggccgtc ttgtcctcga
     3481 tgaagggatt ggcgtcgatc ttcttctgga tgttctccca ggcgaattcg ctcttcggga
     3541 tcggcccgat gatgccgaag ttgttgcgcg tcggcatcag gaacaccggg atggcgccgg
     3601 tcatgatgat cgagtgcagg atcgacttgt ggcagttgcg gtcgacgacg acgatgtcgc
     3661 ccggcgccac ggtcgagttc cagacgatct tgttcgaagt cgaggtgccg ttggtgacga
     3721 aatacaggtg atcgcagttg aagatgcggg cggcgttgcg ctcgcttgcc gcgaccggac
     3781 cggtatggtc cagcagttgg cctagttcgt cgacggcgtt gcagacatcg gcgcgcagca
     3841 tgttctcgcc gaagaactgg tggaacatct ggccgaccgg cg
//
LOCUS       DAOSDX010000167         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8256_length_3880_cov_3.490196, whole genome shotgun sequence.
ACCESSION   DAOSDX010000167 DAOSDX010000000
VERSION     DAOSDX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8256_length_3880_cov_3.490196"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLN02_04795"
     CDS             complement(<1..766)
                     /locus_tag="PLN02_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOI51671.1"
                     /translation="MKLLLAVLLSALGLAWGWDVYNAAPTAAHPWWVARQELLYLSGV
                     LAIGLMSLATYLASRPVWLETPLGGMDRVYRTHKWAGILGGSFAVLHWLVEMSDDILK
                     SSIGRSGRVPKEQFGNVLDRLRDLAEDLGEWGFYIVIALIVLTLWQRFPYRPWRFLHR
                     AMPVIYLALAFHAVLLAPADYWTQPLGLLLAPLVAAGCYGALRALLANIGRERRVSGK
                     VAELEPLGSDLLRVRCQLDAGWPGHRVKTMRRKKNFC"
     gene            complement(763..1173)
                     /locus_tag="PLN02_04800"
     CDS             complement(763..1173)
                     /locus_tag="PLN02_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOI51672.1"
                     /translation="MQISKFPRRLVLALSLGGIIAVGAVLADNHRERRHDERGDAGWL
                     PIPALISRLEAAGYRNVEEIEREHGRYEVRVTDRNGERRKLYFDGRSGEPLGAGRAEG
                     VPRERVVQPADGRECNQRRCRDDLPATPAGGAKP"
     gene            complement(1207..1698)
                     /locus_tag="PLN02_04805"
     CDS             complement(1207..1698)
                     /locus_tag="PLN02_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HOI51673.1"
                     /translation="MKFPLRPFALSLLALGFSALVYTSAQAGGGHYYPPVKDPVVKEE
                     CGSCHLAFPAAMLPAASWQKMMGELDRHFGDNATVDPALAQKITAYLVANAADTGGSR
                     YGAKLMRGVDPASAPQRITKLPKWVREHREVPDWEWRHKDVRTRANCVACHADAELGY
                     YED"
     gene            complement(1715..2125)
                     /locus_tag="PLN02_04810"
     CDS             complement(1715..2125)
                     /locus_tag="PLN02_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HOI51674.1"
                     /translation="MSRRLILSLIVAALPLAASAGALEDQLARYATAAKAETPGFSGF
                     SAARGEKLYFTEFGLGKPDTPACATCHGKDPRAAGQARSGKRIDPVALSVSPGRYADP
                     AKVEKWFKRNCDEVLGRACTAQEKGDWLSFVASR"
     gene            2262..2558
                     /locus_tag="PLN02_04815"
     CDS             2262..2558
                     /locus_tag="PLN02_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51675.1"
                     /translation="MSKPFFFLLLFASAGACAADAADPATASASGNPCPAIRARIAAQ
                     SGVLSQPDSVLLKEIGSHQACRFTRDEVYRAGFGERPPAPAEHHYRRWHDDDDD"
     gene            2685..3032
                     /locus_tag="PLN02_04820"
     CDS             2685..3032
                     /locus_tag="PLN02_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51676.1"
                     /translation="MSERKFRTVLLAALLCLAGNIAQAADPATVNANQLMTQEERVEQ
                     RNKMRAAKTEEERAAIRAENHQRMSERAAAQGKVLVPVNPQGPQQAGGRRGQGPGAGP
                     GAGRGMGAGQGRQ"
     gene            3038..3373
                     /locus_tag="PLN02_04825"
     CDS             3038..3373
                     /locus_tag="PLN02_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51677.1"
                     /translation="MRTTSRRTLLGFALALTLIAPSVADERQDHEVARRALAAGEILP
                     LGAVLERIGREHRGEVLEVELEHEDEQWIYEVKMLRRDGGVNKLIVDARDGRLLEVKG
                     RRGRRGGRH"
     gene            3373..>3880
                     /locus_tag="PLN02_04830"
     CDS             3373..>3880
                     /locus_tag="PLN02_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI51678.1"
                     /translation="MRILLVEDEPTLNLQIGRSLRAEGYIVEVAVNGRDALHLGNEED
                     FDAVVLDLGLPLLDGISVLKRWRANGRGMPVLILTARGDWHERVAGIDAGADDYLVKP
                     FHMEELAARLRALIRRAAGQAANELRCGPLSLDLRGARVCLDGQPLTLTSHEFRVLAY
                     LMQRRGQAV"
BASE COUNT          636 a         1333 c         1331 g          580 t
ORIGIN      
        1 ggcagaaatt cttcttcctc ctcatcgtct tcacccggtg ccccggccag ccggcatcaa
       61 gctggcatcg cacgcgcagc aggtcgctgc cgagcggctc aagttcggcc accttgccgc
      121 tcacccggcg ttcccggccg atgttggcga gcagggcgcg cagcgcgccg tagcagccgg
      181 cggcgacaag cggcgcaagc agcaggccga gcggctgcgt ccagtagtcg gcgggcgcca
      241 gcagcacggc gtggaaggcg agcgccaggt agatgaccgg catcgcccgg tgcaggaaac
      301 gccacggacg gtagggaaag cgttgccaca gggtcagcac gatcagcgcg atgacgatgt
      361 agaagcccca ttcgccgagg tcctcggcca gatcgcggag gcggtcgaga acgttgccga
      421 actgctcctt gggcacccgc ccgctgcgcc cgatcgacga tttcaggatg tcgtcggaca
      481 tctccaccaa ccagtgcagg acggcgaaac tgccgccgag gatgcccgcc cacttgtgcg
      541 tgcgatagac gcggtccatg ccgccgagcg gcgtttccag ccagaccggc cggctcgcca
      601 ggtaggtggc gagcgacatc aggccgatgg cgaggacgcc gctcaggtag agcagttcct
      661 ggcgcgccac ccaccacgga tgggcggcgg tcggcgcggc gttatagacg tcccagcccc
      721 aggcgaggcc gagagcggag agcaggacgg cgagcagcag cttcatggct tggcgcctcc
      781 ggccggcgtt gccggcaggt cgtcgcggca gcgccgctgg ttgcattcgc ggccgtcggc
      841 cggctgcacc acccgctcgc gcggtacgcc ttcggcgcgg ccggcgccga gcggctcgcc
      901 actgcggccg tcgaagtaga gcttgcgccg ctcgccgttg cggtcggtga cgcgcacttc
      961 gtaacggccg tgttcgcgct cgatttcctc gacattgcgg tagccggccg cttccagccg
     1021 gctgatcagc gccgggatcg gcaaccagcc ggcgtcgccg cgttcgtcgt gacggcgttc
     1081 gcggtgattg tcggcgagca cggctccgac agcgatgatg ccgcccaagc tcagcgccag
     1141 cacgaggcgg cgcgggaatt tactgatctg catgattttt cctcccgggt cgccggcggc
     1201 tgccggtcaa tcctcgtagt agccgagttc ggcatcggcg tggcaagcca cgcaattggc
     1261 cctggtccgc acgtccttgt ggcgccattc ccagtccggc acctcgcggt gctcgcgcac
     1321 ccacttcggc agcttggtga tgcgctgcgg cgcgctggcc gggtcgacgc cgcgcatcag
     1381 cttggcgccg tagcggctgc cgccggtatc ggcggcgttg gccaccaggt aggcggtgat
     1441 cttctgggcg agggccggat cgaccgtggc gttgtcgccg aagtggcggt cgagctcgcc
     1501 catcatcttc tgccacgaag ccgccggcag catcgccgcc gggaaggcga ggtggcagct
     1561 gccgcactct tccttgacca cggggtcctt gaccggcggg tagtaatggc cgccgccggc
     1621 ctgcgcgctg gtatagacca gggcggaaaa gccgagcgcc agcaaggaca gcgcgaaggg
     1681 acgaagggga aatttcatgg cgttctcctg acgatcagcg actggcgacg aagctcagcc
     1741 agtcgccctt ttcctgggcg gtgcaggcgc gaccaaggac ttcgtcgcag ttgcgcttga
     1801 accatttctc gaccttggcc gggtcggcgt aacgccccgg cgacaccgac agcgccaccg
     1861 gatcgatacg cttgccgctg cgcgcctggc cggcagcgcg cggatccttg ccgtggcagg
     1921 tggcgcaggc cggcgtatcg ggcttgccga ggccgaattc ggtgaaatac agcttctcgc
     1981 cgcgggcagc agagaagccg gagaaacccg gggtttccgc cttggcggcg gtggcgtagc
     2041 gggccagctg gtcttcgagg gcaccggcgc tggcggcgag cggcagggcg gcgacgatga
     2101 gcgagaggat gaggcggcgt gacatctggc aatactcctg aatcggtggc gatgcagcgc
     2161 actgtgcccg atgccgcctg aatgaaagct gaaggcagca ttcaggcagg attcatcgcc
     2221 gggcgacgag gatgagcgcc attgccaacc gggagcccgt catgtccaag ccttttttct
     2281 tcctgctgct gttcgccagt gccggcgcct gtgccgccga cgccgccgat ccggcaaccg
     2341 cttccgccag tggcaacccc tgccctgcca tccgcgcccg catcgccgcc cagagcggcg
     2401 tcctcagcca acccgacagt gtcctgctca aggaaatcgg cagccaccag gcatgccgct
     2461 tcacccgcga cgaggtctat cgcgccggct tcggtgagcg gcccccagcg ccggccgaac
     2521 accattaccg gcgctggcac gacgacgatg acgactgatc ggcgttcagg cgaggttcag
     2581 cttgccccgc gtaaaacagt aagcggggaa tgaagaaagc ctgtatccgg tcatggcggg
     2641 cacgtcgccc gcgtgacatc attctgatcg tgaaggagtg catcatgtcc gaacgtaaat
     2701 tccgtaccgt cctgctggcc gccctattgt gcctcgccgg caacatcgcc caggccgccg
     2761 atccggccac cgtcaacgcc aaccagttga tgacgcagga agagcgcgtc gaacaacgca
     2821 acaagatgcg cgcggccaag accgaagagg aacgcgccgc gatccgcgcc gaaaaccacc
     2881 agcgcatgag cgaacgcgcc gcggcgcaag gcaaggttct ggttccggtc aatccgcagg
     2941 gaccgcagca ggccggcggt cgtcgcggcc agggccccgg cgccggtccg ggtgccggtc
     3001 gcggcatggg tgccggccag ggtcgccagt aaagtccgtg cgtaccacca gccgccgcac
     3061 cctgcttggg ttcgccctcg ccctgacgct gatcgcgccg tccgtcgccg acgaacgcca
     3121 ggatcacgaa gtggcgcggc gggcgctggc ggccggtgaa atcttgccgc tcggcgccgt
     3181 cctcgaacgg atcggccgcg aacaccgcgg cgaagtcctt gaggtcgagc tggaacacga
     3241 ggacgagcaa tggatctacg aagtgaagat gctgcgccgc gacggcggcg tcaacaagct
     3301 gatcgtcgac gcccgcgacg gccgtctgct tgaagtcaag ggccgccgcg gacgccgggg
     3361 aggacggcac tgatgcgcat cctgctggtc gaggacgaac cgacgctgaa cctgcagatc
     3421 ggccgcagcc tgcgcgctga aggctacatc gtcgaagtcg ccgtcaatgg ccgcgacgcc
     3481 ctgcacttgg gcaacgagga ggatttcgac gcggtcgtgc tcgacctcgg cctgccactg
     3541 ctcgacggca tctcggtgct caagcgctgg cgcgccaacg gccgcggcat gccggtgctg
     3601 atcctgaccg cgcgcggcga ctggcacgag cgcgtcgccg gcatcgacgc cggcgccgac
     3661 gattacctgg tcaagccctt ccacatggaa gaactggctg cccgcctgcg cgcgctgatc
     3721 cggcgcgccg ccggccaggc cgccaacgaa ctgcgctgcg ggccgctcag cctcgacctg
     3781 cgcggcgccc gcgtctgcct cgacggccag ccgctgacgc tgaccagcca cgaattccgc
     3841 gtcctcgcct acctgatgca gcggcgcggc caggccgtca
//
LOCUS       DAOSDX010000168         3876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8264_length_3876_cov_3.701125, whole genome shotgun sequence.
ACCESSION   DAOSDX010000168 DAOSDX010000000
VERSION     DAOSDX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8264_length_3876_cov_3.701125"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..736)
                     /locus_tag="PLN02_04835"
     CDS             complement(20..736)
                     /locus_tag="PLN02_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51679.1"
                     /translation="MFLRDARNIRDLMTQAVERVREAGQGKRGKMDIGIFGSGILDVI
                     PKVLHAFRARHPDVQIALHQMSKKEQIEALRQHRIIAGFNRMLAPLPDIASEPVMIES
                     IMVALNEKNPLAAKPAILLKELAGQPLILFPSGARPSFIDKVWALCAGEGFEPQVAQE
                     VGDAPTSVALVAAGFGLSLVPEAASSLSVPGVVYRRLANAPDAVVDLSCIYRSDEGSP
                     LLHAFLQTIREFREKHGGRH"
     gene            complement(751..921)
                     /locus_tag="PLN02_04840"
     CDS             complement(751..921)
                     /locus_tag="PLN02_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI51680.1"
                     /translation="MEFRQLKYFIAVAEELNIGRAANRLHISQPPLTRQIQQLEEEFG
                     TQLFRARRAASN"
     gene            complement(935..2611)
                     /locus_tag="PLN02_04845"
     CDS             complement(935..2611)
                     /locus_tag="PLN02_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOI51681.1"
                     /translation="MTIIRYPESNDLRNLVKFCEKDGTIWLAENRMVLMHTTALGALR
                     RELVGSVGKEHARRLLTRMGYAAGVSDAELAKRIRGNQSLQDAFFTGPQLHMLEGVVR
                     VTPVEMEIDFEKGSFYGEFLWENSWEQDIHLRELGRSEDPVCWSQIGYASGYTSAFMG
                     RFILFKEVECVACGHNNCRIVGKPLEEWEDAEEHASYFESDSMLNRLLELRHQVDYLR
                     TTIAQQVQTQQLVGNSVGFKHAYDLVTRASGTQVTVLLLGETGVGKERFARTLHQMSQ
                     RKQGPFVAVNCAAMPNDLIESELFGVEKGAFTGAQTSRMGKFERADGGTLFLDEIGEL
                     PLAAQAKLLRVLQEGEIERLGDDRVRKLNIRLVAATNVDLQAAVKEGRFRSDLYYRLS
                     VYPIQIPPLRERIADIPPLIDAMLARFCTLYEKKLLGVSDKAMQAIKSYGWPGNVREL
                     ENMIERGIILAPNGGWIEIEHLFANVAVSDFHETQISQAGSLEAQPEPGAKSDLIAAI
                     LDSGIAFDDLETRLIDEAVKRADGNLAGAARTLGITRPQLQYRLKKKDGG"
     gene            2957..3223
                     /locus_tag="PLN02_04850"
     CDS             2957..3223
                     /locus_tag="PLN02_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase subunit"
                     /protein_id="HOI51682.1"
                     /translation="MGQQERQSSTFDAGRRYVRICKRRPDGFIEFEFAIGDPELAVEL
                     MLPESAFHEFCLANEVIVLDPATSEGDWVSRLNDASRQGFEDAV"
     gene            3271..>3876
                     /locus_tag="PLN02_04855"
     CDS             3271..>3876
                     /locus_tag="PLN02_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol hydroxylase"
                     /protein_id="HOI51683.1"
                     /translation="MQIDLRTVAIKPLRHTFDHLVDRFGDKPASRYQEGSYNIQQEHI
                     FHYRPSWDADNVLFDKKRTKIRMQDWYALKDPRQYYYGSYTIARARQQEAAESSFEMI
                     EDRGLAELIPAELRQTALDVLVPMRHVEWGGNMGNSYIAAYGYGTVITQPCLYHAMDH
                     LGVAQYLTRIGLVLDGPDALETAKSAWMSCPNWQPLREHVEN"
BASE COUNT          636 a         1157 c         1316 g          767 t
ORIGIN      
        1 cggcgggctg aggcggcgct cagtgtcgcc cgccgtgttt ttccctgaat tcccggattg
       61 tctgcaggaa ggcgtgcagt agcggcgagc cttcgtcgct gcggtagatg cagctgagat
      121 cgacgaccgc gtcgggggcg ttggccaggc ggcggtagac gacgcccggc acgctcaggc
      181 tgctggccgc ttccgggacc aggctgaggc cgaagccggc ggcgaccagg gcgacgctgg
      241 ttggcgcgtc gccgacttcc tgggcgacct gcggctcgaa accttcgccg gcgcacaggg
      301 cccagacctt gtcgatgaag ctggggcggg cgccgctcgg aaacaggatg agcggctggc
      361 cggccagttc cttgagcagg atcgccggct tggcggcaag cgggtttttt tcgttgaggg
      421 cgaccatgat gctttcgatc atcaccggtt cgctggcgat gtcgggcagc ggcgccagca
      481 tgcggttgaa accggcgatg atgcggtgct ggcgcagggc ttcgatctgt tccttcttgc
      541 tcatctggtg cagggcgatc tgcacgtcgg ggtggcgggc gcggaaggcg tgcagcacct
      601 tgggaatgac gtcgaggatg ccggagccga agatgccgat gtccatcttg ccgcgcttgc
      661 cctgaccggc ctcgcggacg cgctcgacgg cctgggtcat caggtcgcgg atgttgcggg
      721 cgtcgcgcag gaacatttcg ccggcttcgg tcagttcgac gccgcgcgcc gtgcgcggaa
      781 gagctgggta ccgaactcct cctccagttg ctggatctgc cgcgtcagcg gcggctgcga
      841 gatgtgcagg cggttggcgg cgcggccgat gttgagttct tcggcgacgg cgatgaagta
      901 cttgagttgg cggaattcca tgccgcgtgc tccgtcagcc gccgtccttc ttcttcaggc
      961 ggtattgcag ttgcggccgg gtgatgccga gcgtgcgcgc ggcgccggcc aggttgccgt
     1021 cggcgcgctt gacggcttcg tcgatgaggc gggtttccag gtcgtcgaag gcgatgccgc
     1081 tgtcgaggat ggcggcgatc aggtcggact tggcgccggg ttccggttgc gcttccaggc
     1141 tgcccgcctg gctgatctgc gtttcgtgga agtcgctgac ggcgacgttg gcgaacaggt
     1201 gctcgatctc gatccagccg ccgttggggg cgaggatgat gccgcgttcg atcatgtttt
     1261 ccagttcgcg cacgttgccc ggccagccgt agctcttgat cgcctgcatc gccttgtcgc
     1321 tgacgccgag cagcttcttc tcgtagagcg tgcagaagcg ggcgagcatg gcatcgatca
     1381 gcggcgggat gtcggcgatg cgttcgcgca gcggcggaat ctggatcggg tatacgctca
     1441 ggcggtagta gaggtcggag cggaagcggc cttccttgac cgcggcctgc aggtcgacgt
     1501 tggtggcggc gacgaggcgg atgttcagct tgcgcacgcg gtcgtcgccc agacgctcga
     1561 tctcgccttc ctgcagcacg cgcagcagct tggcctgggc ggcgagcggc agttcgccga
     1621 tctcgtcgag gaagagggtg ccgccgtcgg cgcgttcgaa cttgcccatg cgcgaggttt
     1681 gcgccccggt gaaggcgcct ttctcgacgc cgaacagttc ggattcgatc aggtcgttag
     1741 gcatcgccgc gcaattgaca gcgacgaacg gcccctgttt gcgctggctc atctggtgca
     1801 gggtgcgcgc gaagcgctcc ttgccgacgc cggtttcgcc gagcaggagg acggtgacct
     1861 gggtgccgga ggcgcgcgtc accaggtcgt aggcatgctt gaaaccgacc gaattgccga
     1921 ccagttgctg cgtctgcacc tgttgcgcga tggtcgtgcg caggtaatcg acctgatggc
     1981 gcagttcaag caggcgattg agcatcgagt cggattcgaa gtagctggcg tgctcttcgg
     2041 cgtcttccca ctcttccagc ggcttgccga cgatgcggca gttgttgtgg ccgcaggcga
     2101 cgcactcgac ttccttgaac aggatgaaac ggcccatgaa ggccgaggtg tagcccgagg
     2161 cgtagccgat ctgcgaccag cagacggggt cttccgaacg ccccagttcg cgcaggtgga
     2221 tgtcctgttc ccaggagttc tcccagagga attcgccgta gaagctgcct ttctcgaagt
     2281 cgatttccat ctcgaccggc gtcacgcgca cgacgccttc gagcatgtgc aactgcgggc
     2341 cggtgaagaa ggcatcctgc agcgactggt tgccgcggat gcgcttggcc agttcggcgt
     2401 cgctgacgcc ggcggcgtag cccatgcggg tcagcaggcg gcgcgcgtgt tccttgccga
     2461 ccgagccgac cagttcgcgg cgcagggcgc cgagcgccgt ggtgtgcatc agcaccatgc
     2521 ggttttcggc aagccagatc gtgccgtcct tctcgcaaaa cttgacgagg ttgcgcaggt
     2581 cgttcgattc cgggtaacgg atgatggtca tcttgtctct ctgtcctggc gggcttgccc
     2641 gggtggatgg gggcctcttt ttattggggt gctggcgccg gcagggcgcc gtaattttct
     2701 cgagattatt tcacggtttc ccttttcgtg ggtcatatct ccatacgtaa ttccagactc
     2761 gccgccagac cagcgctgtt gatcatttag ttaatcatcg ggaaatcagt tcattgaatc
     2821 gtaacgatgc accgcagcat tgcgctggtc tggttttgct gatttccata aaaacaatgc
     2881 cttagttgtc tggcacgctc cttgataaag aagacgcggc agcgatgtgc gctgtgcgga
     2941 tttcaggaga cagaagatgg gacagcagga gaggcagagc agcacgttcg acgccgggcg
     3001 tcgctacgtc cgtatctgca agcgtcgtcc ggatggattc atcgaattcg agttcgcgat
     3061 cggcgatccg gagctggctg tcgagctgat gttgccggaa tcggcatttc acgagttctg
     3121 cctggccaac gaggtgatcg tgctcgatcc ggcaacgtcg gagggcgact gggtttcccg
     3181 gttgaacgat gcctcgcgtc agggctttga agacgcagtt tgacgaagtt tcaacacccc
     3241 cgttcgcggg aataacgaca ggagacaaac atgcagattg atctgcgcac cgtggcgatc
     3301 aaacccttgc gccacacctt cgaccatctg gtcgaccggt tcggcgacaa gccggccagc
     3361 cgctaccagg aaggcagcta caacatccag caggaacaca tcttccacta ccggccgagc
     3421 tgggacgccg acaacgtgct gttcgacaag aagcgcacca agatccggat gcaggactgg
     3481 tacgcgctga aggacccgcg ccagtactac tacggctcct acaccatcgc ccgcgcgcgg
     3541 cagcaggaag cggccgaatc gagcttcgag atgatcgagg atcgcggtct ggccgaactg
     3601 attccggccg aactgcggca gaccgcgctc gatgtcctgg tgccgatgcg ccacgtcgag
     3661 tggggcggca acatgggcaa ctcctacatc gccgcctacg gctacggcac ggtcatcacc
     3721 cagccctgcc tctaccacgc gatggaccac ctgggcgttg cccagtacct gacccgcatc
     3781 ggtctcgtcc tcgacggccc ggacgcgctg gaaaccgcca agagcgcctg gatgtcgtgc
     3841 ccgaactggc agccgctgcg cgagcacgtc gaaaac
//
LOCUS       DAOSDX010000169         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8266_length_3875_cov_3.771728, whole genome shotgun sequence.
ACCESSION   DAOSDX010000169 DAOSDX010000000
VERSION     DAOSDX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8266_length_3875_cov_3.771728"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLN02_04860"
     CDS             complement(<1..259)
                     /locus_tag="PLN02_04860"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOI51684.1"
                     /translation="MILCIDSGNSRIKYGLWDGVGWCRSGSCAQADGAALGEIRGEWP
                     MPRRIMLANVAGAGAEAGIRAALGDWGGRLEVVRSTAQAAGV"
     gene            complement(256..1050)
                     /locus_tag="PLN02_04865"
     CDS             complement(256..1050)
                     /locus_tag="PLN02_04865"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOI51685.1"
                     /translation="MPLIDPVLLKARLGPLAGRFDVDALAECDSTSSELLRRAERGAP
                     SGTVIVADRQLAGRGRRGRSWLSSADGCLMFSVLWRSQRGLAGLGGLSLAVGVALVRA
                     LERLGASGVRLKWPNDVLLATAAGDAKLAGILIELAGGRREAQVCIGIGLNLQTPAGE
                     FAQPVAGLAQALQVLPERHALLATILGALLGVLDDFAECGFAAVKDEWQARHAWQDRA
                     VRLIEDGCAERSGICRGVADDGALLLETATGCERILAGDVSLRVNA"
     gene            complement(1061..2473)
                     /gene="ntrC"
                     /locus_tag="PLN02_04870"
     CDS             complement(1061..2473)
                     /gene="ntrC"
                     /locus_tag="PLN02_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HOI51686.1"
                     /translation="MKPVWIVDDDRSIRWVLEKTLSREGIPFASYASASEAIARLESG
                     EEAPQVLVSDIRMPGQSGLDLLQEVKNRHPGVPVIIMTAYSDLESAVAAFQGGAFEYL
                     PKPFDVDQALELIRRAIDESMHQSGAAEEEGLVPEILGQAPAMQEVFRAIGRLALSHA
                     TVLITGESGSGKELVARALHRHSPRAERPFVAINTAAIPKDLLESELFGHERGAFTGA
                     QAQRRGRFEQAEGGTLFLDEIGDMPSELQTRLLRVLSDGHFYRVGGHSPIKANVRVIA
                     ATHQNLESRVKDGLFREDLFHRLNVIRIRLPALRERREDIPLLTRHFLQKSARELGVE
                     PKRLSEGALKYLCGLEFQGNVRQLENLCHWLTVMAPGQQVEVGDLPSELREAPPQVLV
                     GDWEKALEGEVDRLLARGVPDVHGLLSQSFERILISRALAHTGGRRIEAAQALGIGRN
                     TITRKIADLGIDGGKEAAGE"
     gene            complement(2518..3579)
                     /gene="glnL"
                     /locus_tag="PLN02_04875"
     CDS             complement(2518..3579)
                     /gene="glnL"
                     /locus_tag="PLN02_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HOI51687.1"
                     /translation="MGVTHANFAGLDLLASAVLLIDDDQCIRYLNPAGENLLAVSSRS
                     VLGRRLSEVCKCSSTLQSAIDNGLQNNWGYTGQNIALGRADGETLHLNCTVTPLRPDI
                     AAGMRLLLELQPIEHHLAATREERLLEQQQANRELIRNLAHEIKNPLGGIRGAAQLLE
                     HELGNPALKEYTQVIIKEADRLQDLMQRLLTPHRAMLPTTVNIHEILERVRSLLMAEF
                     SEGLKIRRDYDISLPELVGDREQLIQAVLNIARNAAQAMAGQGEIVLRTRALRQVTLA
                     KKRYRMALELKVIDNGPGIPEDIRERMFYPLVSGREGGNGLGLTIAQNFVQHHHGSID
                     CYSRPGQTVFTIRLPIEPA"
     gene            3667..>3875
                     /locus_tag="PLN02_04880"
     CDS             3667..>3875
                     /locus_tag="PLN02_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOI51688.1"
                     /translation="MTVAIRASRLLLLAVLASLGACGKAPERTEDKRTVMVHIATAAP
                     SSGMQLTGEVRARHEVELAFRVGGK"
BASE COUNT          609 a         1322 c         1281 g          663 t
ORIGIN      
        1 gcacgccggc ggcctgcgcc gtgcttcgca ccacctcgag ccgcccgccc caatcgccga
       61 gcgcggcacg gatgcccgcc tcggcgccgg caccggcgac attggcgagc atgatgcggc
      121 gcggcatcgg ccactcgccc cggatttcgc ccagggcggc gccgtcggcc tgcgcgcaac
      181 tgccgctgcg acaccagccc acgccgtccc acaggccgta cttgatgcgg ctgttgccgc
      241 tgtcgatgca gaggatcatg cgtttacccg caggctgacg tcgccggcga ggatgcgctc
      301 gcagccggta gcggtttcca gcagcaaggc gccgtcgtcg gcgacgccgc ggcagatgcc
      361 gctgcgctcg gcgcagccat cttctatcaa ccgcacggcc cggtcctgcc aggcatggcg
      421 cgcctgccac tcgtccttga cggcggcaaa gccgcactcg gcgaagtcgt cgagcacgcc
      481 gagcagcgca ccgagaatcg tcgccagcag tgcgtggcgt tcgggcagta cctgcagcgc
      541 ctgcgccagc ccggcgaccg gctgcgcgaa ctcgcctgcc ggcgtctgca ggttgaggcc
      601 gatgccgatg cagacctgcg cctcgcgccg gccgccagcc aactcgatca ggatgccggc
      661 cagcttggcg tcaccggccg cggttgcgag cagcacgtca ttcggccatt tcaagcgaac
      721 gccgctggcg cccaggcgtt ccagcgcccg caccagggcg acgccaaccg ccagcgacag
      781 gccgccgagg ccggccaggc cgcgctggct gcgccagagg acggaaaaca tcaagcagcc
      841 gtcggcgctc gataaccagc tacgcccgcg ccggccacga ccggcaagct gacggtcggc
      901 gacgatgacg gtaccggagg gcgcgccacg ctcggcgcgg cgcaacaatt cgctgctggt
      961 cgagtcgcac tcggccagcg catcgacgtc gaaacggccg gccaggggac cgagccgcgc
     1021 tttcaacagg acaggatcga tcaggggcat gcggaagatt ttactctccc gccgcctcct
     1081 tgccgccgtc gatgccgaga tcggcgatct tgcgggtgat cgtattgcgg ccgatgccca
     1141 gcgcctgcgc cgcctcgatg cggcggccgc cggtgtgggc cagcgcgcgc gagatcagga
     1201 tgcgctcgaa gctctgcgac aagaggccat gcacgtcggg cacgccgcgc gccagcaggc
     1261 ggtcgacctc gccttccagc gccttttccc agtcgccgac cagcacctgc ggcggcgcct
     1321 cgcgcaattc gctcggcagg tcgccgactt cgacctgctg ccctggcgcc atcacggtca
     1381 accagtgaca gagattttcc agttgccgga cgttgccctg aaactccaga ccgcaaagat
     1441 acttgagcgc gccctcggac agccgcttcg gttcgacacc cagttcgcgc gcgctcttct
     1501 gcaggaagtg gcgggtgagc agcgggatgt cctcgcgccg ttcgcgcagc gccggcaggc
     1561 ggatgcggat gacgttgagg cgatggaaga gatcctcgcg gaacagcccg tccttgaccc
     1621 ggctttccag gttctggtgg gtcgcggcga tgacgcggac gttggccttg atcggcgaat
     1681 gcccgccaac ccggtagaag tggccatccg acagcacgcg cagcaggcgg gtctgcagtt
     1741 cggaaggcat gtcgccgatt tcgtcgagga acagcgtgcc gccctcggcc tgctcgaaac
     1801 ggccgcgccg ctgcgcctgg gcgccggtaa aggcgccgcg ctcgtggccg aagagctcgg
     1861 attcgagcag gtccttggga atcgccgcgg tattgatcgc cacgaagggg cgctcggcac
     1921 gtgggctgtg ccggtgcagc gcgcgcgcca ccagctcctt gccggaaccc gattcgccgg
     1981 tgatcagcac ggtggcgtgc gacagcgcga ggcggccgat ggcgcggaag acttcctgca
     2041 tcgccggcgc ctggccgaga atctcgggca ccagcccctc ttcctcggcc gcaccactct
     2101 ggtgcatcga ctcgtcgatc gcccgccgga tcagttccag cgcctgatcg acgtcgaagg
     2161 gcttgggcag atattcgaag gcgccgccct ggaaggcggc gaccgcgctt tccagatccg
     2221 agtaggcggt catgatgatg accggcacgc cagggtggcg gttcttcact tcctgcaaca
     2281 gatcgagccc ggactggccg ggcatgcgaa tgtccgagac cagcacctgc ggcgcttcct
     2341 caccgctctc cagacgggcg atcgcctcgc tcgccgaagc atagctggcg aacggaatac
     2401 cctcgcgcga caaggtcttt tccagcaccc agcgtatcga gcggtcgtcg tcaacgatcc
     2461 ataccggttt catgtcatgc ctcgttctct ttgtttgtcc gattgcttta gcaagactca
     2521 ggccggctcg atgggcaagc gaatggtgaa taccgtctgc cccggccggc tgtagcagtc
     2581 gatgctgccg tgatgatgct ggacgaaatt ctgggcgatc gtcagaccca agccgttgcc
     2641 gccctcgcgc ccggagacca gcggatagaa catgcgctcg cggatatcct cggggatgcc
     2701 cgggccgttg tcgatgacct tgagttccag cgccatgcgg tagcgcttct tggccagcgt
     2761 cacctggcgc agcgcccgcg tgcggaggac gatctcgccc tggccggcca tcgcctgcgc
     2821 cgcgttgcgg gcgatgttga ggacggcctg aatcagttgc tctcgatcgc cgaccagctc
     2881 gggcaggctg atgtcgtagt cgcggcgaat cttcaggcct tcggaaaact cggccatcag
     2941 caggctgcgc acccgttcga ggatttcgtg gatgttcacc gtcgtcggca gcatcgcccg
     3001 gtgcggcgtc agcaagcgct gcatcaggtc ctgcaggcgg tcggcctcct tgatgatgac
     3061 ctgggtgtat tccttgagcg cagggttgcc gagttcgtgt tcgagcagtt gtgccgcgcc
     3121 gcggatgcca ccgagcggat tcttgatttc gtgcgccagg ttgcggatca gttcacggtt
     3181 ggcctgctgc tgttcgagca ggcgttcctc gcgcgtcgcc gccaggtggt gctcgatcgg
     3241 ctgcagttcg agcagcaggc gcatgccggc ggcgatgtcc gggcgcaacg gggtcaccgt
     3301 gcagttgagg tgcagggttt cgccgtcggc gcggccgagg gcgatgttct ggccggtgta
     3361 gccccagttg ttctgcaaac cgttgtcgat cgccgactgc agtgttgacg agcacttgca
     3421 gacctcgctc aagcgccgcc cgagcacgct ccgactactg accgcgagca ggttttcacc
     3481 agccgggttg aggtagcgga tgcattggtc gtcgtcgatc agcaataccg ccgacgccag
     3541 gagatcgagc ccggcgaaat tggcgtgggt cacgcccatg cgacgccccc gcaactacgc
     3601 tgccttgctt gctttaatga cccgctgttc attatcatcc tggaactcct ttttcccgtc
     3661 tgcgccatga ccgtcgccat ccgcgcctcc cgcttgctgc tgctcgccgt gctggcttcc
     3721 ctgggcgcct gcggcaaagc cccggagcgc acagaagaca aacgcaccgt catggtgcat
     3781 attgccacag ccgcaccatc atcgggcatg cagctgactg gtgaggtccg cgccaggcac
     3841 gaggtggagc tggctttccg ggttggcggc aagat
//
LOCUS       DAOSDX010000170         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8267_length_3875_cov_2.979843, whole genome shotgun sequence.
ACCESSION   DAOSDX010000170 DAOSDX010000000
VERSION     DAOSDX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8267_length_3875_cov_2.979843"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLN02_04885"
     CDS             complement(<1..481)
                     /locus_tag="PLN02_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOI51689.1"
                     /translation="MNLIIQGGALPTFLLDRILSATGATAVEPLPPRVVRLREARRTA
                     EFDALIPLIEAEKLDWAFVEAGRKLSDFGLICFDMDSTLITIECIDELADFAGKKREV
                     SEVTEAAMRGEIDYRESLRRRLALMGGLDARVLARVFGERLLLSDGARELLEGVQNAG
                     "
     gene            complement(548..1075)
                     /locus_tag="PLN02_04890"
     CDS             complement(548..1075)
                     /locus_tag="PLN02_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI51690.1"
                     /translation="MPTLLIFPGIGNSGPEHWQSRWERANPDFVRIAQREWDNPVCAE
                     WTAVLESTVQRLGPSVVIVAHSLACLAVAHWAARPHSPVRAALLVAVPDPDGAAFPVE
                     ASGFSPLPMQPFSFPSIVVSSSDDPYGSSEHAAACAAAWGSRLVEIGAAGHINASSNL
                     GDWPEGLALLQQLCV"
     gene            1177..1434
                     /locus_tag="PLN02_04895"
     CDS             1177..1434
                     /locus_tag="PLN02_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOI51691.1"
                     /translation="MHTWPVQDAKARFSEFLDACLQEGPQMVTRRGAETAVLVPVQEW
                     RRLQTAARPSLKQLLLSEQARVDLIVPARGLAKRRTVEQMP"
     gene            1431..1841
                     /locus_tag="PLN02_04900"
     CDS             1431..1841
                     /locus_tag="PLN02_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOI51692.1"
                     /translation="MTTIYLLDTNVVSELRRPRPHGGVLAWLESLDDAQLHLSAVTLG
                     EIQAGIEITREQDPDKAKEIEAWLELVAGAYNVLPMDATVFRAWAKLMHGKSDTLYED
                     GMIAATAKVHGLTVATRNVVDFNVFGVEVFNPFA"
     gene            complement(1856..2800)
                     /locus_tag="PLN02_04905"
     CDS             complement(1856..2800)
                     /locus_tag="PLN02_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI51693.1"
                     /translation="MFDWRDYGNGIHAFDAGYVRPILAAIHLLVDDGRVALIDTGSND
                     SLPNVLAALEKLGLGRDAVDYIILTHIHLDHAGGAGTLMRELPNARLVVHPRGSRHMA
                     EPSKLVAGVTAVYGQDYVDRVYGEILPIAAERIIDAHEGLTLQLGGRELLCIDTPGHA
                     KHHICVVDRKSGGIFTGDMFGLSYREFDVDGRQFILPTTSPTQFDPVEMHASIDRLMS
                     FAPTAMYLTHYAQVHDVATQAAALRQRLDSHVAIAEAHANAGAERHAKIKQGITDALL
                     ADLRTHGCTLPEAEILDVLATDLELNAQGLAYWLDHRQ"
     gene            2882..3715
                     /locus_tag="PLN02_04910"
     CDS             2882..3715
                     /locus_tag="PLN02_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI51694.1"
                     /translation="MVHFPADDGELIHLHVSGEGSPLILLHGWTSSHQEWTPFLASLA
                     ARHRVYRWDARGHGGHKLAHAGSATVERMARDLKNLIDHYKIVNATAVGHSMGALTLW
                     QYLRDHGSHGLGKLCLIDQSPRLLTDDDWDHGIYGNFGADRNRQLIDWLHEDFAEGVL
                     KLTALGLNQRARQKYEENASGWERLRQALRGQNPEPLIHCWQSLTAADYRDVLARISL
                     PTLLVYGGQSNFYGGETARYVADTIPNAILNIYEGSDHSPHQWQRERFVRELLEFIDG
                     E"
BASE COUNT          698 a         1200 c         1282 g          695 t
ORIGIN      
        1 ggccggcgtt ctgcacgcct tcgagcagtt cgcgggcgcc gtcggaaagc agcaggcgct
       61 cgccgaaaac gcgcgccagc acgcgggcat cgagcccgcc catcagcgcc aggcggcggc
      121 gcaggctctc gcggtaatcg atctcgccgc gcatcgccgc ctcggtcact tccgacacct
      181 cgcgcttctt gccggcgaaa tcggccagct cgtcgatgca ctcgatggtg atcagcgtcg
      241 aatccatgtc gaagcagatc aggccgaaat cggacaactt gcgcccggcc tcgacgaagg
      301 cccagtcgag cttttccgcc tcgatcagcg gaatcagcgc gtcgaactcg gccgtccgtc
      361 gcgcctcgcg caggcgcacc acgcgcggtg gcaagggctc gacagccgtt gcgccggtgg
      421 cgctcagaat gcggtcgagc aggaaagtgg gcagcgcgcc gccctggatg atcaggttca
      481 tggaggggac ttgggaaatc attgattctg ttgttttctg gtgagacgtt tcgcccgaca
      541 tcatcgttca gacgcaaagc tgctgcaaca aggcgaggcc ctccggccag tcgccgagat
      601 tgctgctggc gttgatgtga ccggcggcgc cgatttcgac caggcgactg ccccacgccg
      661 cagcgcaagc cgccgcgtgc tcggacgaac cgtagggatc gtcgctgctg gacacgacga
      721 tgctgggaaa ggaaaacggc tgcatgggca gcggcgagaa gccgcttgcc tcgaccggaa
      781 aagccgcgcc gtcgggatcg ggcaccgcca ccagcaacgc cgcccggacc ggcgaatgcg
      841 gccgcgccgc ccagtgcgcc acagccagac aggccaggct atgagcgacg atgaccaccg
      901 acggcccgag ccgctgcacg gtgctttcca gcacggcagt ccattccgca cagaccggat
      961 tgtcccactc gcgctgcgcg atgcgcacga aatccgggtt ggcgcgctcc cagcgcgact
     1021 gccagtgctc cgggccggaa ttgccgatgc cggggaagat gaggagggtg ggcatgggga
     1081 ttaaggcttg cttgggtaat ggtgctttga aatcggtttg acagtcgttg tcgatgcctg
     1141 cagactaggc agatgactag ttttagcgag gttactatgc atacatggcc agttcaggat
     1201 gcgaaagcgc gcttcagcga gtttctggat gcctgcctgc aggaaggccc ccagatggta
     1261 acgcgaagag gcgccgaaac ggcagtgctg gtgcccgtcc aggaatggcg gcgtctgcag
     1321 acggcggcac gtccctccct gaaacaattg ctgctctccg agcaggctcg cgttgacctg
     1381 atcgtcccgg cgcgcgggct ggcaaagcgc cgcactgtcg agcaaatgcc gtgacgacca
     1441 tctacctgct ggacacgaac gtggtttccg aactgcgcag gccgcgcccg catggcggcg
     1501 tgcttgcctg gctggaatcg ctggacgacg cccagctcca cctgtccgcc gtgactttgg
     1561 gcgagatcca ggccggcatc gagataacgc gggaacaaga cccggacaag gccaaggaaa
     1621 tcgaggcctg gctggaactg gtggcggggg cctacaacgt cctgccgatg gatgcgacgg
     1681 tattcagggc ctgggctaaa ttgatgcacg gaaagtccga tacgctttac gaagacggaa
     1741 tgatcgccgc gacggcgaaa gtacacgggc tgacggtggc gacaagaaat gtcgtggatt
     1801 tcaatgtctt tggcgttgag gtgttcaacc cctttgccta gttgttgctt cgacgttact
     1861 gccgatgatc cagccagtaa gccagcccct gcgcgttcag ttcgaggtcg gtcgccagga
     1921 cgtcgaggat ttcggcttcg ggcaaggtgc agccgtgcgt ccgcaggtcg gccagtagcg
     1981 cgtcggtgat gccctgcttg atcttcgcgt gtcgctcggc gccagcattg gcgtgagctt
     2041 cggcaatcgc cacatggctg tcgaggcgct ggcgcaaggc cgcggcctgc gtcgccacat
     2101 cgtgcacctg agcatagtgg gtcaggtaca tcgccgttgg ggcaaagctc atcaggcggt
     2161 cgatcgaggc atgcatttcg acggggtcga actgtgtcgg cgaggtcgtc ggcaggatga
     2221 actggcggcc gtcgacgtcg aattcgcggt acgacaggcc gaacatgtcg ccggtgaaaa
     2281 tgccgccgct cttgcggtcg acgacgcaga tgtgatgctt ggcgtggccc ggcgtgtcga
     2341 tgcagagcag ttcccggccg ccgagttgca aggtcagtcc ttcgtgcgcg tcgatgatcc
     2401 gttcggcggc gatcggcagg atttcgccat agacccgatc gacatagtcc tgaccataga
     2461 cggcggtgac gccggcgacc agcttggacg gttcggccat gtggcgggaa ccgcgcggat
     2521 gcacgaccag tctggcgttc ggcagttcgc gcatgagcgt gccggcgccg ccggcatggt
     2581 cgaggtggat gtgggtcagg atgatgtaat cgactgcatc gcgcccgagg ccgagttttt
     2641 cgagtgccgc caggacgttg ggcagcgaat cgttgcttcc ggtatcgatc agggcgaccc
     2701 gcccgtcatc gacgagcagg tgaattgccg ccaggatcgg ccgcacatag cctgcatcga
     2761 aggcatggat gccgtttccg taatcgcgcc agtcaaacat gagttctcct cgttgggttt
     2821 tttagtattt gggggccccg attatactgg cgggcctccg actttctgcg aacccagcgc
     2881 catggtgcat ttcccggccg acgacggcga gctcatccat cttcatgtct ccggcgaagg
     2941 ttcgccgttg attctgttgc atggctggac atcgagtcat caggaatgga cgccgttcct
     3001 cgcttcgctc gccgcccggc accgggtcta tcgttgggac gcccgcggtc acggcgggca
     3061 caagctggcc catgccggtt cggcgacggt cgagcggatg gcccgcgacc tgaagaacct
     3121 gatcgaccac tacaagatcg tcaacgcgac ggcggtcggc cattcgatgg gcgcgctgac
     3181 gctgtggcaa tacctgcgcg accacggctc gcacggcctg ggcaagctgt gcctgatcga
     3241 ccaatcgccc aggctgctta ccgacgacga ctgggaccat ggcatctacg gcaatttcgg
     3301 cgccgaccgc aaccggcagc tgatcgactg gctgcacgag gatttcgccg aaggcgtcct
     3361 gaagctgacc gcgctcggcc tcaaccagcg tgcccggcag aaatacgaag aaaacgccag
     3421 cggctgggag cgcctgcgcc aggctttgcg cggccagaac ccggagccgc tgattcactg
     3481 ctggcaaagc ctgactgccg ccgactaccg cgacgtgctg gcgcggattt cattgcccac
     3541 gctcctcgtc tatggcgggc agagcaattt ctacggcggc gaaacggcgc gttatgtcgc
     3601 cgacacgatt cccaacgcca tcctcaacat ctacgaaggc agcgaccatt cgccgcatca
     3661 gtggcagcgc gaacgcttcg tgcgcgagtt gctggaattc atcgacggcg agtgattgct
     3721 ccttcgccgg gggcagactg tcggcgggca gatgcccgcc gttgtcggtt ggatcagtcg
     3781 gcgatgcgct ccggcagcac gtcgcgcagc aggaccgccg cgtcgcgcag catcttctcg
     3841 gtggtgtccc agccgacgca gccgtcggtg accga
//
LOCUS       DAOSDX010000171         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8299_length_3863_cov_2.797269, whole genome shotgun sequence.
ACCESSION   DAOSDX010000171 DAOSDX010000000
VERSION     DAOSDX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8299_length_3863_cov_2.797269"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1250)
                     /locus_tag="PLN02_04915"
     CDS             complement(96..1250)
                     /locus_tag="PLN02_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI51695.1"
                     /translation="MHTNKPTLIAALVGSLLLAACSDKNAPAAPQMGPMPVTVLDMQP
                     QSVPTSIEVMAQTEGARETEVRARVGGILVKRLYQEGDVVKSGQPLFQIDRAPYEIAL
                     AEAKARAEQTAREKQRLKGLADAKAISQKDYDDATSADAMAQAALRQAELNLSWTTVT
                     APVGGTSGRAVRSEGNLVSTGSDSLLTSIYQTNPMWVSFSLGESDLAKLPGGRVTENL
                     VSGVELVMANGQVYEQKGKLNFVASNIDTTLGTQALRAEFANPDGQLLPGQFVRIRLL
                     TGQRDGIFLVPQAAVLQTEQGTLVMTADGESKVAPRPVKTGEWHGKDWVILGGLQAGD
                     KVIVDNLMKLRPGVPVAPHPPGAPGAPGAGAAPGGKPGAAPAAGNAAAQK"
     gene            1389..2006
                     /locus_tag="PLN02_04920"
     CDS             1389..2006
                     /locus_tag="PLN02_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOI51696.1"
                     /translation="MVRKTKEEAEQTRKDIVTAARKVFHECGVSRSSLDKIAKAAGVT
                     RGAIYWHFQDKAEVFFAMRDEVFEPMLGCTDAVLYDPSLLNPLDAIEGSIKEFFRVLA
                     TCPDFRETFEIMISRCEYVEEFSSVQEEVGRPALEFLEKIQRMYRLAAEKGYLRAGLE
                     PEAVAHDTWAFTSGLLHLTLGCRKNSGLEREIPQMISAHMDLRRA"
     gene            complement(2011..2889)
                     /gene="rarD"
                     /locus_tag="PLN02_04925"
     CDS             complement(2011..2889)
                     /gene="rarD"
                     /locus_tag="PLN02_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOI51697.1"
                     /translation="MSHKTPLAGIGYGLLAYGIWGFFPLYFRQLSHVSAIDILANRAL
                     WACVCVCLILVVAQQWAKVRQILGRPRQLAMLTLAALLIGSNWLVFLWAVEQHRVVES
                     SLGYFLTPLVNVVLGLVFLKERLNRLEWLAIALAAAGLANEIAAHGGLPWVSLFLAGS
                     FGTYGLVRKQIPVDALSGLFLETLLMLPLCLAYAAWQWQAGHTVFTIAPWSTGALLFG
                     AGILTAAPLIAFAAATQRLDLATVGMLMYINPTMQFLTAIYVFGEALQTEKLISFSLI
                     WIGLLVFSYSAWRKYR"
     gene            complement(2892..3584)
                     /locus_tag="PLN02_04930"
     CDS             complement(2892..3584)
                     /locus_tag="PLN02_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HOI51698.1"
                     /translation="MHALPHLSTLDWLSLTVFFACWGGYAWFSEHSAGGAGGLIRTSQ
                     RYRLDWAYRMLERDVRVTDSTLIGNLMTSVSFYAYTTIYIIAGLVAALGAADQLLGFA
                     ADLPFAGPGNRELLEIKLMLVLASFVFAYFKFTWSLRQFNILSILVGAAPTGSAGDPA
                     IDRYARRVAGTNTLAGDDFNRGIRGYYFGLAAAGWLLHPLILGGLALAVCGILYRRDY
                     RSAALAVLRDNA"
     gene            3713..>3863
                     /locus_tag="PLN02_04935"
     CDS             3713..>3863
                     /locus_tag="PLN02_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI51699.1"
                     /translation="MSDLANAHESASDLIGIEALVQGTPVATFVIDSEHRVTHWNRAC
                     ELVTGL"
BASE COUNT          708 a         1116 c         1343 g          696 t
ORIGIN      
        1 acctgcgcgg cgaccaggcc ggcgatgacg atgatgatcg agatgaccga agcgaagatc
       61 ggtcgattga tgaagaacct ggacatgatc gccccttatt tctgtgccgc agcgttgccg
      121 gcggccggtg ccgcacccgg cttgccgccg ggggcagcgc ctgcaccggg tgcgccgggc
      181 gcgccgggcg gatgcggggc gaccggcacg cccggtcgca gcttcatcag gttgtcgacg
      241 atgaccttgt cgccagcctg caggccgccc aggatgaccc agtccttgcc gtgccattcg
      301 ccggtcttga ccgggcgcgg cgccaccttg ctctcgccgt cggcagtcat caccagcgtg
      361 ccctgttcgg tctgcaacac cgccgcttgc gggaccagga agatgccgtc gcgctggccg
      421 gtgagcaagc ggatgcggac gaactggccg ggcagcaact ggccgtcggg gttggcgaac
      481 tcggcgcgta gcgcctgggt gccgagcgtg gtgtcgatgt tcgaagcgac gaaattgagc
      541 ttgcccttct gttcgtaaac ctggccgttg gccatcacca gttcgacgcc gctgaccagg
      601 ttttcggtga cgcggccgcc gggcagcttg gcgaggtcgc tctcgccgag cgagaagctg
      661 acccacatcg ggttggtctg gtaaatcgag gtcagcaggc tgtcgctgcc ggtgctgacc
      721 aggttgcctt cggagcgcac ggcgcggccg ctggtgccgc cgaccggcgc ggtcaccgtg
      781 gtccacgaca ggttcagttc ggcctggcgc agcgccgcct gtgccatcgc gtcggcggaa
      841 gtggcgtcgt cgtaatcctt ctggctgatc gccttggcgt cggccagccc cttcagccgc
      901 tgtttctcgc gcgctgtctg ctcggcacgg gctttcgctt cggccagtgc aatttcgtaa
      961 ggtgcgcggt cgatctggaa cagcggctgg ccggacttga cgacgtcgcc ttcctgatag
     1021 aggcgcttga ccaggatgcc gccgacgcgg gcccggactt cggtctcgcg ggcgccttcg
     1081 gtttgcgcca tgacctcgat cgaggttggc accgactgcg gttgcatgtc gaggacggtg
     1141 accggcatcg gtcccatctg cggtgcggcc ggtgcattct tgtccgaaca agcggcgagc
     1201 agcaggctgc cgacaagggc ggcgatcagg gtaggtttgt tggtatgcat ggttggggtt
     1261 ctccggcggg gaagttttgg gctattatat acaaacacga atgtatgtaa aacgcctcag
     1321 tggcttacag caaagcattt ttgtgttgca aattgtagtt gctgccggcc ggcagcaggg
     1381 agaacgagat ggtcagaaaa accaaggaag aagctgagca gacgcgcaag gatatcgtca
     1441 ccgcagcgcg caaggtcttc cacgaatgcg gtgtcagccg ttcgtcgctg gacaagatcg
     1501 ccaaggcggc cggtgtcacg cgtggtgcca tttactggca tttccaggac aaggcggaag
     1561 tcttttttgc gatgcgcgac gaggtattcg agccgatgct cgggtgcacc gacgccgtgc
     1621 tttacgatcc ttcgctgctt aatcctctgg atgcgatcga gggttcgatc aaggaatttt
     1681 tccgcgtcct cgcgacctgc ccggattttc gcgagacctt cgaaatcatg atttcgcgct
     1741 gcgaatacgt cgaggaattt tccagcgttc aggaagaagt tggccggccg gcgctggaat
     1801 tcctggaaaa gattcaacgc atgtaccgac tggcggccga aaagggctac ctccgtgccg
     1861 gattggagcc cgaggctgtg gcccacgata cctgggcttt taccagcggg ctattgcacc
     1921 tgaccctggg gtgtcgcaaa aacagcggtc tggaaaggga gattccccag atgatttcag
     1981 cgcatatgga tttgcgccgg gcctgacgct tcagcggtac ttgcgccagg cgctgtagct
     2041 gaacaccagc aggccgatcc agatcaggct gaaagagatc agtttttccg tttgcagcgc
     2101 ttcgccgaag acgtagatgg cggtcaggaa ctgcatcgtc ggattgatgt acatcagcat
     2161 gccgacggtc gccaggtcga ggcgctgcgt cgccgccgcg aaggcgatca gcggcgccgc
     2221 agtgaggatg ccggcgccga atagcagggc gccggtgctc cagggggcga ttgtgaagac
     2281 tgtatgtccg gcctgccatt gccacgctgc gtaggccagg cagagcggta acatgagcag
     2341 ggtttcgagg aacaggccgg acagcgcgtc gaccggtatc tgcttgcgca ccaggccgta
     2401 ggttccgaag ctgccggcca ggaagagcga gacccagggc aggccgccgt gggcggcaat
     2461 ctcgttggcg agtccggccg ccgccagggc gatcgccagc cattccaggc ggttgagccg
     2521 ctccttcagg aacaccagcc cgagcaccac gttgaccagc ggcgtcagga agtagccaag
     2581 gctggattcg accacccggt gctgttccac cgcccacagg aaaaccagcc agttgctgcc
     2641 gatcagcagc gccgccagcg tcagcatggc gagttggcgc gggcggccga ggatttgcct
     2701 gacctttgcc cattgttgtg cgacgaccag gatcaggcag acgcagacac aggcccagag
     2761 tgcacggttg gccaggatgt cgatcgccga gacgtgactc agttggcgaa aatagagcgg
     2821 aaagaagccc cagatgccat aggcgagcag accatagccg atgccggcca gtggggtttt
     2881 gtggctcatg ctcaagcgtt gtcgcgcagt acggcgagcg ccgccgaacg gtagtcgcgg
     2941 cggtagagga tgccgcagac ggcgagcgcc aggccgccga ggatcagcgg atggagcagc
     3001 cagccggcgg cggcgaggcc gaagtaatag ccgcggatgc cgcggttgaa atcgtcgccg
     3061 gccagcgtgt tggtgccggc cacccgccgc gcgtagcggt cgatcgcggg gtcgccggcg
     3121 ctgccggtgg gggcggcgcc gaccaggatg gagaggatgt tgaactggcg cagcgaccag
     3181 gtgaacttga agtaggcgaa gacgaaactg gcgagcacca gcatcagctt gatttccagc
     3241 agctcgcggt tgcccggccc ggcgaacggc aggtcggcgg cgaagccgag caactggtcg
     3301 gcagcgccga gcgcggcgac gaggccggcg atgatgtaga tggtggtgta ggcgtagaag
     3361 gagacgctgg tcatcaggtt gccgatcagc gtcgagtcgg tgacgcggac gtcgcgctcc
     3421 agcatgcggt aggcccagtc gaggcggtag cgctggctgg tgcggatcag gccgccggca
     3481 ccgccggcgc tgtgctcgga aaaccaggcg tagcctcccc agcaggcgaa gaatacggtc
     3541 agcgacagcc agtcgagcgt ggagaggtgg ggcagggcgt gcatggcgcc agtttgccac
     3601 aagggcggtt cggcgagggg aggcgatcag aggtgaaatg gcgtaaaaaa caaaagtgat
     3661 gttttgagct gttgctttac aatagcgtcg tttttgttaa ttaagttgat tcatgagtga
     3721 cttggcgaat gcccacgaaa gtgcctcgga cctgatcggc atcgaggccc tggttcaagg
     3781 gacccccgtg gcgaccttcg tcatcgacag cgaacaccgg gtcacccact ggaaccgggc
     3841 ctgcgagttg gtcaccgggt tgc
//
LOCUS       DAOSDX010000172         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8324_length_3856_cov_3.230992, whole genome shotgun sequence.
ACCESSION   DAOSDX010000172 DAOSDX010000000
VERSION     DAOSDX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8324_length_3856_cov_3.230992"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1508
                     /locus_tag="PLN02_04940"
     CDS             60..1508
                     /locus_tag="PLN02_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HOI51700.1"
                     /translation="MRLTTIAIVLASLPSLAWAAPTWQNISTEAGRSIELDRATLKRT
                     GNVVEALSRVTLDRPLSDSRTGMPYRLIEATTRYDCASRSAKTIKRIYRQAENDVIRE
                     EEIAGAELPVRTGTLDDRVLREVCRPPKEDPAELAKRANEAASKLQEANAALLKKELG
                     KEAAPMVKTVDKPDALIPSIRPNLKAAMEASRSEAAAPVETRPKAEETPPPAKPKPSL
                     SVSHGTTQLVHNNAAAPRPLKPAPSRANAGYMLELVTPHKPVTWGYEGEGAPENWVKL
                     DPRNVLCAAGKRQSPIDIRDGIKVDLEPIRFDYRPSTFRIVDTGHTVSVAVGDSSFSL
                     TGKTYDLVDIHFHRPGETRINGQRYDMSAHFVHRSIDGQRAVIEVLMTRGNENPQIQT
                     LWNNLPLEKHVPVEPPTAVFEPAALLPGSKQYYTFMGSLTTPPCTEDILWIVFKQPIE
                     VSDEQLRIFSRLYRNNARPAQPIGDRLIKESR"
     gene            1561..2193
                     /locus_tag="PLN02_04945"
     CDS             1561..2193
                     /locus_tag="PLN02_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI51701.1"
                     /translation="MSTAKPVRKRRKEARPGELLAAALTQFVEKGYAATRLDDVAACA
                     GVSKGTLYLYYASKEALFKAVIQEGIVPVLAENEAIAASHSGSSFSLLEKLLDHWWTR
                     IGCTAFAGIPKLIVAEAQNFPEIAGYYYTNVIARGRALVGAALQRGVSSGEFRQCDIE
                     VTIDVIIAPVLMLLIWEYSLGRCEQQPARHRDYLATHLALLQRGLCVPQV"
     gene            2254..2907
                     /locus_tag="PLN02_04950"
     CDS             2254..2907
                     /locus_tag="PLN02_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOI51702.1"
                     /translation="MNIEQARFNMVEQQIRPWDVLDPKVLDLLFIVKREDFVPAAYRN
                     LAFADMEIPLGEGQCMLAPRVEARLLQELGIRQADRVLEIGTGSGYMAALLAARAEHV
                     TTLELRPELAAFARDNLQRAGVANVTVECADGSRGWQQRAPFDVIVLSGALPRIPAEL
                     LKQLRVGGRLAAIVGEAPVMEAQMITCVADGVYNTVNLFETVVPVLDNFVPTREFSL"
     gene            2907..3230
                     /locus_tag="PLN02_04955"
     CDS             2907..3230
                     /locus_tag="PLN02_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI51703.1"
                     /translation="MRQIRASQLAEWLADVNRPPPLLLDVREPWELAQCQIEGVLHVP
                     MHMIPLRCAEFDPQREIVVICHHGGRSMQVCMFLERKGYGAVFNLLGGIEGWACEVDP
                     GMNRY"
     gene            3238..>3856
                     /locus_tag="PLN02_04960"
     CDS             3238..>3856
                     /locus_tag="PLN02_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI51704.1"
                     /translation="MKQIAWLFAASLTAAPLHAADLAQIYRAAQDNDPVYAAARATLD
                     AGREKAPQGLAGLLPTVTASGNTVWNDVESEARNAPAAASNVEYNSYAYQLTLSQPLF
                     RWQNWVAYGQAKQQVAQAEATFVQASQDLILRVAQAYFDVLYASENLTAVRANKEAIA
                     QQLELAKKSFEVGTATITDTHEAQARFDLVQAQEIAAESELEVRRQ"
BASE COUNT          767 a         1202 c         1166 g          721 t
ORIGIN      
        1 ggtcgaaata agttcgacaa gcccgtgaaa attcgaagaa aacctacagg aaacaaccca
       61 tgcgtttgac gaccattgcc atcgttctgg ccagtctgcc ttctctggcc tgggctgcgc
      121 caacctggca gaacatttcc accgaggccg gtcgcagcat tgaacttgat cgtgcgaccc
      181 tgaagcgcac cggcaatgtc gtcgaagcct tgagccgggt gacactcgat cgtccgctat
      241 ccgacagccg gaccgggatg ccgtaccggc tcatcgaggc gaccactcgt tatgactgcg
      301 cgtcgcgcag tgcgaagaca atcaagcgca tctatcggca agccgaaaac gacgtcatca
      361 gggaagaaga gattgccggc gccgaactgc cggtgcgtac cgggacgctc gacgaccgcg
      421 tcctgcgcga ggtctgccgt ccgcccaagg aagatccggc cgagctcgcc aagcgcgcca
      481 acgaagcggc gagcaaactg caggaagcca acgcggcttt gctgaagaag gagttgggga
      541 aggaagcggc cccgatggta aaaaccgtcg ataagcccga tgcgctgatt ccgtcgattc
      601 gcccgaatct caaggcggca atggaagctt cgcgcagtga agcggcggcg ccggtcgaga
      661 cccggccgaa agccgaggaa acgccgccgc cagccaagcc gaagccgagt ctcagcgttt
      721 cacatggaac gacccaactg gtccacaaca atgcggcggc gccgcgcccg ctgaagccgg
      781 cgccgagccg tgccaacgcg ggctacatgc tggagttggt gacgccgcac aagccggtga
      841 cctggggcta cgaaggcgaa ggtgcgccgg agaactgggt caagctcgat ccgcgtaacg
      901 tcctctgtgc cgccggcaag cgtcagtcgc cgatcgatat ccgggatggt atcaaggtcg
      961 atcttgagcc gattcgtttc gattaccgac cctccacgtt ccgcatcgtc gatactggcc
     1021 acaccgtttc ggtcgcggtt ggcgacagca gtttctccct gactggcaag acctacgatt
     1081 tggtggacat ccatttccat cgcccgggcg agacccggat caacggccag cgttacgaca
     1141 tgtccgccca ttttgtgcat cgctccatcg acggtcagcg ggcggtgatc gaggtgctga
     1201 tgactcgtgg taacgagaat ccgcagatcc agaccctgtg gaataatctg ccgctcgaga
     1261 aacatgttcc ggtcgagccg ccgacggccg tcttcgagcc ggccgctctt ctgcccggta
     1321 gcaagcagta ctacaccttc atgggatcgc tgacgacacc cccttgtacc gaagatatcc
     1381 tgtggatcgt cttcaagcag ccgatcgagg tttcggacga gcaactgcgg atcttcagcc
     1441 gcctttatcg caacaacgcc cgtcccgccc agccgatcgg cgaccggctc atcaaggaga
     1501 gccgttgacc ctagccgtga tggggtgtta gcttgatgac ccaatagtca ttaagccgcc
     1561 atgagtaccg ccaaaccagt ccggaaacgt cgcaaggaag ctcgccctgg cgagttgctg
     1621 gcggccgcgt tgacccagtt cgtcgagaaa ggctacgccg ccacccgcct cgatgacgtt
     1681 gccgcctgtg ccggagtttc caaggggaca ctctaccttt actacgccag caaggaagct
     1741 ttgttcaagg cggtgatcca ggaaggcatc gtgccagtcc tggcggaaaa cgaggcgatt
     1801 gccgccagtc actccggcag cagcttctcc ttgctggaaa agttgcttga tcattggtgg
     1861 acacgcatcg gttgcactgc cttcgccggc attcccaagt tgatcgtcgc cgaggcgcag
     1921 aactttcccg agatcgccgg ctactactac accaatgtga tcgcgcgtgg tcgtgccctg
     1981 gtcggcgccg cgttgcagcg tggcgtcagc agcggcgaat tccggcagtg tgacatcgaa
     2041 gtcacgatcg acgtcatcat tgccccggtg ctgatgttgc tgatctggga atattcgctg
     2101 gggcgctgcg agcagcagcc cgcccgtcac cgtgactatc tcgccacgca tctggcgctg
     2161 ttgcagcggg ggctgtgtgt gcctcaggta taatccgatt ttgtcagaac caatatatta
     2221 gcgtatgcta atattaagtt tgcggagaat ttcatgaaca tcgaacaagc gcgcttcaac
     2281 atggtggagc aacagattcg tccttgggac gtgctcgacc cgaaagtgct cgacctgctc
     2341 ttcatcgtca agcgcgaaga tttcgtgccg gcggcctatc gcaacctggc cttcgccgac
     2401 atggaaatcc cgctgggcga aggtcagtgc atgcttgctc cccgcgtcga agcccgcctg
     2461 cttcaggaac ttggcatccg ccaagctgat cgcgtgctgg aaatcggtac cggcagcggt
     2521 tatatggcag cgcttctggc agcgcgcgcc gagcacgtga cgaccctcga actgcgcccg
     2581 gaactggcgg cgtttgcccg cgacaacctg cagcgtgccg gtgtcgccaa tgtcaccgtc
     2641 gaatgtgccg atggcagccg cggttggcag caacgcgcgc ccttcgatgt gatcgtgctt
     2701 tccggcgccc tgccgcggat tcccgccgaa ctcctcaagc agttgcgggt tggcggccgc
     2761 ctggcggcaa tcgtcggcga ggcgccggtg atggaagctc aaatgatcac gtgcgtcgcc
     2821 gacggtgtgt acaacacggt caatctgttc gagacggttg ttcccgttct tgacaacttc
     2881 gtcccgacgc gcgagttcag cctgtaatgc gccagattcg ggcatcccaa ctggcggaat
     2941 ggctggccga tgtgaatcgg ccgccgccgt tgctgctcga tgtccgcgag ccctgggagc
     3001 ttgcgcagtg ccagatcgaa ggggtgctgc atgtgccgat gcacatgatt ccgctgcgtt
     3061 gtgccgagtt cgatccgcag cgtgaaatcg tcgtcatctg tcaccacggc ggtcgcagca
     3121 tgcaggtctg catgtttctc gaacgcaagg gttacggcgc cgtgttcaac cttctgggtg
     3181 gcatcgaggg ctgggcttgc gaagtcgatc ccggaatgaa ccgctactga ggtccgcatg
     3241 aaacaaatcg cctggctttt tgccgcttcc ctgaccgccg ctccgctgca tgccgccgat
     3301 cttgcccaga tctaccgcgc cgcgcaggac aacgacccgg tatacgccgc cgcccgggca
     3361 acgctcgatg ctggtcgcga aaaagcgccg cagggactgg ccggcttgct gccgacggtg
     3421 accgcttccg gcaacacggt ctggaacgac gtcgaatccg aagcgcgcaa cgccccggct
     3481 gctgccagca atgtcgaata caacagctac gcctatcagc tgaccttgtc gcaaccgctg
     3541 ttccgctggc agaactgggt cgcctacggt caggcaaagc agcaggtggc gcaggccgaa
     3601 gcgactttcg tgcaggccag ccaggatctg atcctgcggg tggcgcaggc ctacttcgac
     3661 gtcctctacg ccagcgaaaa cctgaccgcg gtacgcgcca acaaggaagc gatcgctcag
     3721 cagctcgaac tggcgaagaa gagtttcgag gtcggtacgg cgacgatcac cgacacccat
     3781 gaagcgcagg cccgcttcga cctcgttcag gcgcaggaaa tcgctgccga gagcgagctt
     3841 gaagtgcgcc gccagg
//
LOCUS       DAOSDX010000173         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8354_length_3846_cov_3.311000, whole genome shotgun sequence.
ACCESSION   DAOSDX010000173 DAOSDX010000000
VERSION     DAOSDX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8354_length_3846_cov_3.311000"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..2700)
                     /locus_tag="PLN02_04965"
     CDS             complement(196..2700)
                     /locus_tag="PLN02_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI51705.1"
                     /translation="MKPRKTLSRILLANMLLVSTLACVLIGSLWIAQEILAFNAEVAA
                     MSERLLAERKARMQQEVDAAAAYLEFMRGQTEERTRRIIRERTLEAHRLATYLYDHYK
                     NKLAPAELQDMVREALRPIRFLDGRGYYFATRLDGMEMLCAACPELEQKNLLDLRDAS
                     GAPVIRDMAELVRKDGEGFYRYTWSRPGAPGKEHAKLAYVKHFAPFDWLIGTGEYLED
                     TEGDLQREALDWIRRIRYDGDAYLFAGDWSGVALSGPGTGKNMLAVSDPNGVMIVQEL
                     IRLAQQGSGFVNYLMPKIDGQRPAPKISYARGLPMWKWYIGTGVYIDDIENLVAAARQ
                     RARNELLWNIAKICGILFLLWLAAFGLAGRLNIRTRAMIAEFSGFFSRSADDQTEMPL
                     ASLDIAEFRELAEGANRMITRRRKAEETLREYQEQLEKIVESRTRDLNLAKEAAEAAS
                     RAKSTFLANMSHELRTPMNAIMGMAAQARRRSNEPQLGEHLGKIEQASQHLLAVINDI
                     LDISKIEAERLTLEMTHFRLGEVLEKVISLLDHKAKEKGIALRTELPPEIARLVLIGD
                     PLRLGQVLLNLLGNAVKFTERGSIDLRLRRIDDLPDSVRLRFEVADTGIGITAEDQQR
                     LFTAFEQADGSMTRKYGGTGLGLAISKRLVEMMGGEIGVTSQPGSGSSFWFTVKLQLD
                     TETRLPPPSQPASPPLTQAETRLQTEFAGQRILLAEDEPINREVSCGLLEDVGLCVDI
                     AVDGSQAVDLARQHSYALILMDMQMPCLNGIDATRAIRALPAHADTPILAMTANAFAE
                     DRQACLDAGMNDHLGKPVAPDHLYETLLRWLSKPGA"
     gene            2964..3269
                     /locus_tag="PLN02_04970"
     CDS             2964..3269
                     /locus_tag="PLN02_04970"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOI51706.1"
                     /translation="MFLAMNRFRIALGKEDDFVAHWRNRNSYLHEVPGFVSFNLLRGD
                     SNDERTLFASHTVWESEQAFADWTKSDAFRKAHMQAGQSPRDFYLGPPQLELFEAVL"
     gene            complement(3277..>3846)
                     /locus_tag="PLN02_04975"
     CDS             complement(3277..>3846)
                     /locus_tag="PLN02_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOI51707.1"
                     /translation="QVEIRRDARLDLAIKGLGLLLVAAPWLPGALGAGLALALAALLL
                     IRLPFWHPLRALRRIDIGIMFVGYLAIAGQLLLHAWEYFFGPAWVGTLPTHLFTFGAM
                     GCVIPAMIVRIAKGHTGRKVSFDAADRGVLYLMLAALAIRTLLPQLAPTHYLLWIDAA
                     ALCWLLAFGLLAWRYVPLLLAPRIDGREH"
BASE COUNT          625 a         1185 c         1306 g          730 t
ORIGIN      
        1 tggctgtcga cgcggcgctg ctcgaccgcc tgcccgacat ctttccgcag gcctcggggc
       61 gcgacatcaa ggggctggcc aagctggtcg ccaagtactg cagccagaaa caggtggcgg
      121 cgagcgaggc ggtgttccgg cgctgctcga tgttccgggc gctggagcag gcggagtagc
      181 gttggcgggc cgggctcagg ccccgggttt gctcaaccag cgcagcaggg tttcgtacag
      241 gtggtcgggg gcgaccggtt tgccgaggtg gtcgttcatc cccgcgtcga ggcaggcctg
      301 gcgatcttcg gcaaaggcgt tggccgtcat cgccaggatc ggcgtatcgg cgtgggcggg
      361 cagggcgcgg atggcgcgtg tcgcgtcgat gccgttcagg cagggcatct gcatgtccat
      421 caggatcagc gcgtatgagt gttgccgggc aagatcgacg gcctgactgc catcgacggc
      481 aatatccacg cacagtccga cgtcttccag caggccgcaa gagacttcac ggttgatcgg
      541 ctcgtcttcc gccagcagga tgcgctggcc agcgaattcc gtctgcaggc gggtttcggc
      601 ctgcgtcagc ggcgggctgg cgggctggct cgggggcggc aggcgggttt ccgtgtcgag
      661 ctggagcttg acggtaaacc agaagctgct gccgctgccc ggctggctgg tcacgccgat
      721 ttccccgccc atcatctcga ccaggcgctt gctgatggcc agcccgagtc cggtgccgcc
      781 atatttgcgc gtcatcgagc catcggcctg ttcgaaagcc gtgaacaggc gttgctggtc
      841 ttcagcggtg atgccgatgc cggtgtcggc gacttcgaag cgcagccgga cgctgtcggg
      901 gagatcgtcg atccgccgca ggcgcaggtc gatgctgccc cgctcagtga atttgacggc
      961 gttgccgagc aggttgagaa gtacctgacc gaggcgcaaa ggatcgccga tcagcaccag
     1021 gcgggcgatt tccggcggca gttcggtacg cagcgcgatt cccttctcct tcgccttgtg
     1081 atcaagcagg gagatgacct tttccaggac ttcgccgagg cggaaatgcg tcatttccag
     1141 cgtcaggcgt tcggcttcga tcttggagat gtcgagaatg tcgttgatga cggcgagcag
     1201 atgctgcgat gcctgctcga tcttgcccag atgctcgccg agttgcggtt cgttgctgcg
     1261 ccgtctggcc tgtgccgcca tgcccatgat ggcattcatc ggcgtgcgca gttcgtggct
     1321 catgttggcg aggaaggtgc tcttggcgcg acttgccgct tcggccgctt ccttggccag
     1381 attgaggtcg cgggtgcgcg attcgacgat tttttcgagc tgctcctggt actcgcgcaa
     1441 ggtttcctcc gccttgcgcc ggcgcgtgat catgcggttg gcgccttccg ccagttcgcg
     1501 gaattccgcg atgtcgagcg aggcgagcgg catttcggtc tggtcgtcgg cgctgcgact
     1561 gaagaaaccg gaaaactccg cgatcatcgc ccgcgtccgt atgttcagcc ggccggccaa
     1621 cccaaaggcg gccaaccaga gcaggaacag gatgccgcag atcttggcga tgttccagag
     1681 cagctcgttg cgcgcccgtt ggcgggctgc ggcaaccaga ttctcgatat cgtcgatata
     1741 gacgccggtg ccgatgtacc acttccacat gggcaggccg cgggcatagc tgatcttcgg
     1801 tgccgggcgt tgtccgtcga tcttcggcat caggtaattg acgaaaccgc tgccttgttg
     1861 cgccaggcgg atcagttcct ggacgatcat gacgccgttc gggtcgctta ccgccagcat
     1921 gttcttgccg gtgccggggc cggacaaggc cacgccgctc cagtctccgg cgaaaagata
     1981 ggcatcgccg tcgtaacgga tgcggcggat ccagtcgagt gcctcacgct gcagatcgcc
     2041 ttcggtgtcc tccaggtatt cgccggtgcc gatcaaccag tcgaaagggg caaagtgctt
     2101 cacataagca agcttggcgt gttccttccc cggggcgccc ggtctgctcc aggtgtagcg
     2161 gtagaaccct tcgccatcct tcctgaccag ttccgccatg tcgcggatga ccggggcgcc
     2221 gctggcgtcg cgcaggtcga gcaggttttt ctgctcgagt tccgggcagg cggcgcacag
     2281 catttccatg ccatcaagcc gggtggcgaa atagtagccg cgtccgtcga ggaagcggat
     2341 cgggcgcaag gcttcgcgca ccatgtcctg cagttcggcg ggcgccagtt tgttcttgta
     2401 gtggtcgtac aggtaggtgg ccaggcgatg cgcttccagc gtccgctcgc gaatgatgcg
     2461 ccgcgtccgc tcctcggtct ggccgcgcat gaactcgaga taggccgctg ccgcgtcgac
     2521 ttcctgttgc atgcgcgcct tgcgctcggc gagcagacgc tcgctcatcg ccgcgacttc
     2581 ggcgttgaag gcgagaattt cctgggcgat ccacaggctg ccgatcagca cgcaagccag
     2641 cgtcgatacc agcagcatgt tggcgagcag aatccgactc agggtcttgc ggggtttcat
     2701 cggtgcggga aaacggtcgg ggttcttggc aaagtggcag cagtttaagc actaaagacc
     2761 atgtttgcca cgctgtttta ccggcgcgcc aggcctgccc gccgagcttc ccgtgccggc
     2821 cgcaggcagc gcctcccggg gcttcggcaa tgccttgtag aattgccgga ggcggcttcg
     2881 tcactgttgc ggacctttcg ggctgaatcc gtttggcgtg agggtagggg cgttaattcc
     2941 atttgaatcg gtagcttcca tccatgtttc tcgccatgaa ccgtttccgc atcgccctcg
     3001 gcaaggagga cgatttcgtc gcccactggc gcaaccgcaa cagctacctg cacgaggtgc
     3061 cgggtttcgt ctccttcaac ctgttgcgcg gcgacagcaa cgacgagcgc acgctgttcg
     3121 cctcgcatac ggtctgggaa tcggagcagg ccttcgccga ctggacgaag tcggatgcct
     3181 tccgcaaggc gcacatgcag gccggccagt cgccgcgcga tttctatctc gggccgccgc
     3241 aactagagtt gttcgaagcc gtgctctaat cagcgatcag tgctcacggc cgtcgatgcg
     3301 cggcgccagc aggagcggca cgtagcgcca ggcgagcagg ccgaaggcga gcagccagca
     3361 gagggcggcg gcgtctatcc acagcaggta gtgcgtcggc gccagttgcg gcagcagggt
     3421 gcggatcgcc agcgccgcca gcatgagata gagcacgccg cggtcggcgg cgtcgaaact
     3481 caccttgcgc ccggtatgtc ccttggcgat gcgcacgatc atcgccggga tgacgcagcc
     3541 catcgcgccg aaagtgaaca ggtgggtcgg cagggtgccg acccaggccg ggccgaagaa
     3601 atattcccag gcatgcagca gcaattgccc ggctatcgcc aggtagccga cgaacatgat
     3661 gccgatgtcg atccggcgca gcgcccgtag cgggtgccag aagggcaggc ggatcaggag
     3721 cagggcggcg agcgccagtg ccaggccggc gcccagcgcg ccgggcagcc agggggcggc
     3781 caccagcagc aggccgagcc ccttgatcgc caggtcgagc cgggcgtcgc ggcggatctc
     3841 gacctg
//
LOCUS       DAOSDX010000174         3839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8373_length_3839_cov_3.260571, whole genome shotgun sequence.
ACCESSION   DAOSDX010000174 DAOSDX010000000
VERSION     DAOSDX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8373_length_3839_cov_3.260571"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLN02_04980"
     CDS             complement(<1..336)
                     /locus_tag="PLN02_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOI51708.1"
                     /translation="MKSAEIRQKFLDFFAAKGHQIVASSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIKYAWEL"
     gene            complement(450..821)
                     /locus_tag="PLN02_04985"
     CDS             complement(450..821)
                     /locus_tag="PLN02_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51709.1"
                     /translation="MKAIRSLLVVTLSLAVSGLALAGQGSPRVDHEQFRQQQRIEQGV
                     ARGSLTRHETLQLQRGQHRVARMEARAKADGVVTRHERAMLHQAQARESARIQRQKHD
                     RQHARWQDKRWQHERYARYGM"
     gene            820..987
                     /locus_tag="PLN02_04990"
     CDS             820..987
                     /locus_tag="PLN02_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51710.1"
                     /translation="MMILLAGVSTLPIEAPNEQEITPAMQGFVSKGRCRPVAKQLVAT
                     RGALADGGAIG"
     gene            complement(991..1692)
                     /locus_tag="PLN02_04995"
     CDS             complement(991..1692)
                     /locus_tag="PLN02_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOI51711.1"
                     /translation="MLVPPPPRYFAHRGGGTLAPENTLAGIRLAARLGYRAVEFDVML
                     SGSGTPVLIHDETLERTTNGSGAVATTDDAALFALDAGGEPPPRFAQAVRLCGEFGLL
                     ANVEVKPASGFERQTAQRVAAEVRADWLASPLLFSSFSRTALQVLQAEIPDLPRAWLL
                     AALPDDWRAEARRLGVVALHLDLALVTPALVRECAGLGLAVRAYTLQTPALADYLFGI
                     GVAGVFVDPLDRFLP"
     gene            complement(1692..2960)
                     /locus_tag="PLN02_05000"
     CDS             complement(1692..2960)
                     /locus_tag="PLN02_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOI51712.1"
                     /translation="MSQVFRVLLVAAAAVLPFSVAAAKPAPAPAPVPTLAHNLDAANE
                     AKLAAVIERFHKETGGQIKLVRLHKGDKPATLNLLGRYQTEDVFSQPKAFVPLYSMMS
                     KAGVALSVQLSEVLKPSVTDGRGRLVALPVLYSTPVMFYNKLAFRKAGLDPEQPPKTW
                     FEMQGYLDKLQAVGYDCPYASSWPTWVHIDNVSAVSGVPAIDAKTGLKFNGLPQVKHV
                     AMMATWSKAHYFRQYGNADEASARFKEGECAIITTDSREYADFQDARGVELGVAPMPY
                     HDDVYGGRRPTLVGGSSLWVGAGKSAAEYKQAAKFVNYLLSPEIQVELVRNHGGLPLT
                     EAARLASLSRLLQDGRQALEAAYASLRGPGGGPVAQVSRVDDTRLITDEELAAVWADR
                     KPAKAALDTAVMRGNSLLKAQPALKKLQPF"
     assembly_gap    3147..3156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3157..3474
                     /locus_tag="PLN02_05005"
     CDS             <3157..3474
                     /locus_tag="PLN02_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51713.1"
                     /translation="EGKAVGSACRQSGRAIEDCYSIYSWLPKASIYEGWREMDEYMRE
                     NKLETITPQLPPPEPPGSKKKRPAPAPAAAEAEAATVKEGDTAAPATATDNKETTAEH
                     GKS"
     gene            3508..>3839
                     /locus_tag="PLN02_05010"
     CDS             3508..>3839
                     /locus_tag="PLN02_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HOI51714.1"
                     /translation="MNRQPKFLRGIRGKLIAIFVLIKVIPLLLLAWFAWHTARQLGAE
                     VSVKAAGMADASLQTIQEIGKTVTEDSIRALDERSREAIETLTTDAARNIARFLYARD
                     ADILQAAD"
BASE COUNT          692 a         1256 c         1258 g          623 t
ORIGIN      
        1 cagttcccag gcgtacttga tcgcgtcgcg cttgaagtag tcgccgaagc tgaagttgcc
       61 gagcatttcg aagaaggtgt ggtggcgcgc ggtgtagccg acgttttcga ggtcgttgtg
      121 cttgccgccg gcgcgcacgc atttctgcga ggtggtcgcg cggctgtacg ggcgcttgtc
      181 gaagccgagg aagacgtcct tgaactggtt catgccggcg ttggtgaaca gcagcgtcgg
      241 gtcgtcgtgc gggacgagcg acgaggaggc gacgatctgg tgccccttgg cggcgaagaa
      301 gtcgaggaat ttctggcgga tttcggcgct tttcatgaag gttctgcccg gcgggctcgc
      361 taaatacgga atccgtgatt ttaaaagaaa aacccgccgg acggcgggtt tgtccgccca
      421 atacggcggg cgacaccggc agatgcgcat tacatgccgt agcgggcgta acgctcatgc
      481 tgccagcgct tgtcctgcca gcgggcatgc tggcgatcgt gcttctggcg ctggatgcgc
      541 gcactttccc gcgcctgcgc ctggtgcagc attgcccgct cgtgccgggt gacgacgccg
      601 tcggccttgg cccgcgcttc catgcgggcg acccggtgct gcccgcgctg cagttgcagc
      661 gtctcgtgcc gggtcagact accccgggcg acgccctgct cgatgcgctg ctgctgccgg
      721 aactgctcgt gatcgacgcg cggcgagcct tgaccggcca gggcaagacc gctgaccgcc
      781 agggacaggg tgacgacgag aagggaacgg atggctttca tgatgattct ccttgctggg
      841 gtttcgacgc ttcccattga agcgccgaac gagcaggaaa tcacgccggc aatgcaaggc
      901 tttgtaagca aaggtcgctg ccgaccggtg gcgaaacagc ttgttgcaac ccgcggcgcc
      961 ttagccgatg gcggagcgat cggctagcgc ctacggcaga aagcgatcga gcgggtcgac
     1021 gaacaccccg gcaacgccga tcccgaacag gtaatcggcc agcgccggtg tctgcagcgt
     1081 gtaggcgcgc accgccaggc cgagcccggc gcattcgcgg accagtgccg gcgtcaccag
     1141 cgccaggtcg aggtgcagcg cgacgacgcc caggcgccgc gcttctgccc gccagtcgtc
     1201 gggcagcgcg gcaagcaacc aggcgcgcgg cagatcggga atttccgctt gcagcacctg
     1261 cagcgcagtg cgggaaaacg aggaaaacag cagcggcgat gccagccagt ctgccctgac
     1321 ctcggccgct acgcgctgcg cggtctggcg ctcgaatccg ctcgccggtt tgacctcgac
     1381 attggcgagc agcccgaatt cgccgcacag gcggacagcc tgggcaaaac ggggcggcgg
     1441 ttcgccaccg gcgtcaaggg cgaacagcgc cgcgtcgtcg gtcgtcgcga cggcgccgct
     1501 gccgttggtc gtgcgctcca gcgtttcgtc gtggatcagc acgggcgtgc cggagcccga
     1561 caacatgacg tcgaactcga ccgcccggta gccgagtcgc gccgccagcc ggatgccggc
     1621 cagcgtgttt tccggcgcca gcgtgccgcc gccgcgatgc gcaaagtatc gcggcggcgg
     1681 cggcacaagc atcagaaggg ttgcagcttc ttcagcgccg gctgcgcctt gagcaaggaa
     1741 ttgccgcgca tcaccgcggt atcgagcgcc gccttggccg gcttgcggtc ggcccagacg
     1801 gcggcgagct cctcatcggt gatcaggcgg gtgtcgtcga cgcggctgac ctgcgccacc
     1861 ggcccacccc ccggcccgcg tagcgaggcg taggcggctt ccagcgcctg ccggccatcc
     1921 tgcagcaggc ggctgagcga cgccagacgg gcggcttcgg tgagcggcag accgccgtgg
     1981 ttacgcacca gttcgacctg gatttccggc gacagcaggt aattgacgaa cttcgccgcc
     2041 tgcttgtact cggccgccga cttgccggca ccgacccaca gcgacgagcc gccgaccagc
     2101 gtcggccggc ggccgccata gacgtcgtcg tggtaaggca tcggcgcgac ccccagttca
     2161 acgccacggg cgtcctggaa gtcggcgtat tcccgggaat cggtggtgat gatcgcgcat
     2221 tcgccttcct tgaaacgcgc actcgcctcg tcggcattgc cgtactggcg gaaatagtgc
     2281 gccttgctcc aggtggccat catcgccacg tgcttgacct gcggcaggcc attgaatttc
     2341 agaccggtct tggcgtcgat cgccggcacg ccggagaccg cgctgacatt gtcgatatgc
     2401 acccaggtcg gccaggacga agcataagga cagtcgtagc ccaccgcctg cagcttgtcg
     2461 agatagccct gcatttcgaa ccaggtcttc ggcggctgct cggggtcgag cccggctttg
     2521 cggaaagcca gcttgttgta gaacatcacc ggcgtcgaat aaagcaccgg caaggcgacc
     2581 aggcgccccc tgccgtcggt gacgctcggc ttgaggactt ccgacaactg caccgacagg
     2641 gccacgcccg ccttgctcat catcgagtaa agcggcacga aggctttcgg ctggctgaag
     2701 acatcttccg tctgatagcg gccgagcagg ttcagggttg ccggcttgtc gcccttgtgc
     2761 aagcggacca gcttgatctg cccaccggtt tccttgtgga aacgctcgat cacggccgcc
     2821 agcttggctt cgttggcggc atccagattg tgcgccaggg ttggcacggg agccggcgct
     2881 ggcgccggct tggcggcagc gaccgaaaag ggaagcaccg cagcggcggc gaccagtaat
     2941 acacgaaaaa cttgcgacat ctgccccatc cagcaaataa aacgaagcga attatagccc
     3001 ccggaaggac cgccgatttt tcacagttcg tgacagttgt gcgagaatcc ggcgcatgaa
     3061 tctgcgcacc ttgttcctcg ccctctccgt catcgcgctg cccggctgcg accaggtcgc
     3121 cagcaagctc ggcctcgagg acccggnnnn nnnnnngaag gcaaggccgt gggcagcgcc
     3181 tgccgccaat ccggaagagc catcgaggat tgctactcga tctatagctg gttgccgaag
     3241 gccagcattt acgagggctg gcgtgagatg gacgaataca tgcgggagaa caagctggag
     3301 accatcaccc cgcaattgcc gccgccggaa ccgccgggca gcaagaagaa acgccccgcc
     3361 cccgcccccg ccgcagcgga agcggaagca gcgacagtca aagaaggtga taccgccgct
     3421 ccggccaccg ccacggacaa caaggaaacg acagccgaac acggcaaatc gtgatcgact
     3481 gcagcccgcc ggaggaggag ggatgcgatg aatcgccagc caaaattcct gcgcggcatc
     3541 cgcggcaagc tgatcgcgat cttcgtgctg atcaaggtca ttcccctgct gctgctcgcc
     3601 tggttcgcct ggcataccgc acgccagctt ggcgccgagg tttcggtcaa agcggccggc
     3661 atggccgacg cctcgctgca gaccatccag gaaatcggca agaccgtcac cgaggactcg
     3721 atccgcgccc ttgacgagcg ctcgcgtgaa gcgatcgaaa cgctgaccac cgacgctgcc
     3781 cgcaacatcg cccgcttcct ctacgcccgc gacgccgaca tcctgcaggc ggccgacag
//
LOCUS       DAOSDX010000175         3839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8375_length_3839_cov_3.087474, whole genome shotgun sequence.
ACCESSION   DAOSDX010000175 DAOSDX010000000
VERSION     DAOSDX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8375_length_3839_cov_3.087474"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLN02_05015"
     CDS             complement(<1..268)
                     /locus_tag="PLN02_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HOI51715.1"
                     /translation="MTAPVVVFAIGNPSRGDDAIGPLLCARLDSWLLARGLAGGVELI
                     EDFQLNIEHAIDLQGRALALFIDAAVNQEAAVVFRQIGPSATISH"
     gene            complement(265..1731)
                     /locus_tag="PLN02_05020"
     CDS             complement(265..1731)
                     /locus_tag="PLN02_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOI51716.1"
                     /translation="MFELETAAHPETLRRVAIDPVTRVEGHGKVTLLLDQDNKIHQVR
                     LHIVEFRGFEVFIEGRPYWEVPVTVQRLCGICPVSHQLAAVKAMDQIAGYGPLTPTAE
                     KLRRLLHYGQIVQSHAVHFFHLASPDLLLGFDSDFTRRNIVGVALDFPEIAKQGVLLR
                     KYGQEVIRHVAGKRVHGTGAIPGGVNKALSVAERDEMARDIEQMIVWTQGAIDMVRRL
                     HETNPGFYNVFGESPGNMMGLMRADGALDYYHGTLRAVDAAGQTIFDGVDYADYQQVL
                     QEEIKPWSYMKFPHIRSLGADDGWYKVGPLARVQAATFLSTPLAEIERQKMMALGNGK
                     PVHGTLFYHWARLVEVLNAAEMIRDLLHDPDILGSELMADRGERRYEAVGVIEAPRGT
                     LFHHYRVNEDDQVTYCNLIVSTTNNNQAMNEAIRTVARQYLDGRELTEGLLNHIECAI
                     RAYDPCLSCATHALGKMPLLVELVDAGGAVVDRLSHGL"
     gene            complement(1744..2298)
                     /locus_tag="PLN02_05025"
     CDS             complement(1744..2298)
                     /locus_tag="PLN02_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOI51717.1"
                     /translation="MNAPMNPRKLRVTTTSLSGCFGCHMSFLDIDERLLELAALVEFD
                     LSPITDIKHCSQHCDLALVEGGVCNAENVHVLRELRANSKILVAFGACAVNGGLPAQR
                     NHLDVGKCLSQVYRTPDGSATAIPDDPELPLLLDKVYPVNEVVRVDYFLPGCPPSADT
                     IWKFLTDLLTGRTPRIEHPLLKFD"
     gene            complement(2300..3061)
                     /locus_tag="PLN02_05030"
     CDS             complement(2300..3061)
                     /locus_tag="PLN02_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOI51718.1"
                     /translation="MSDTSNANSFSIDGQRIPFTPGQTILQAALAAGVYIPHLCHHPD
                     LKPHGSCKLCTVIVNGRNGSACTQKAMPGIEVENNTPTLNDRRRVLLQMLFVEGNHFC
                     PGCEKSGNCQLQALAYDLEMVSPHFRHAYPVRPIDTTHPDVWLDLNRCIMCELCVRAS
                     RDIDGKNVFMLAGRGAESHIVVNSPSGRLGDSALSIDDAAAHVCPVGAILVKRVGYAV
                     PIGERQFDEQPISRQVDYLARRHIAIVPAAETEER"
     gene            complement(3058..>3839)
                     /locus_tag="PLN02_05035"
     CDS             complement(3058..>3839)
                     /locus_tag="PLN02_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOI51719.1"
                     /translation="ESLEGKAGRPRVRPPFPVTSGYMGKPTVVNNVETLCKVTEIALH
                     GGTWYEAIGTKQSTGTKILSVAGDCARPGLYEYAFGVRIAQVLEDCGAQDAIAVQVSG
                     ASGVCLSEREFGRRIAFEDVPTAGSFMIFGKQRDLFEVARNFAHFFAHESCGFCTPCR
                     VGTVLMKEIMDKIADGRGTQYEMNELLRLQQVLKPGSHCGLGQTAGNAVADTVQKFRP
                     AYERRLAETDFVPAFDLDAALSRARELAGRDDAAAHLGEEA"
BASE COUNT          637 a         1186 c         1336 g          680 t
ORIGIN      
        1 aatgactgat cgtcgccgac ggcccgatct gccggaaaac caccgctgcc tcctgattga
       61 ctgcggcgtc gatgaacaag gcgagcgcgc gtccctgcag gtcgatcgcg tgttcgatgt
      121 tgagctgaaa atcctcgatc agctcgaccc cgcctgccag cccgcgggcc agcagccagc
      181 tgtcgagccg ggcgcagagc agcggcccga tggcgtcgtc gccccggctc gggttgccga
      241 tggcgaagac gacgacgggt gccgtcacag gccgtgactc agacgatcga ccaccgcccc
      301 gccggcatcg accagttcga ccagcagcgg catcttgccc agcgcatggg tggcgcagga
      361 cagacaggga tcgtaagcgc ggatcgcgca ctcgatgtgg ttgagcagtc cctcggtcag
      421 ttcgcggcca tcgagatact ggcgggcgac ggtgcggatc gcctcgttca tcgcctggtt
      481 gttgttggtc gtcgacacga tcaggttgca ataggtaacc tggtcgtcct cattgacacg
      541 gtagtggtgg aagagcgtgc cgcgcggcgc ctcgatgacg ccgaccgcct cgtagcggcg
      601 ctcgccgcgg tcggccatca gctcgctgcc gaggatgtcc gggtcgtgca gcaggtcgcg
      661 gatcatctcg gcggcgttca gcacttcgac cagccgcgcc cagtggtaga acagcgtgcc
      721 gtgcaccggc ttgccgttgc ccagcgccat catcttctgg cgctcgatct cggccagcgg
      781 tgtggacagg aaagtggcgg cctggacgcg ggccagcggg ccgaccttgt accagccgtc
      841 gtcggcaccc aggctgcgga tatgcgggaa cttcatgtag ctccacggct tgatttcttc
      901 ctgcagcacc tgctggtaat cggcgtaatc gacaccgtcg aaaatcgtct ggccggccgc
      961 gtcgaccgca cgcaaggtgc cgtgatagta gtccagcgca ccgtcggcgc gcatcagccc
     1021 catcatgttg cccggcgatt cgccgaacac gttgtagaag ccgggattgg tctcgtgcag
     1081 gcggcgcacc atgtcgatcg cgccctgcgt ccagacgatc atctgctcga tgtcacgcgc
     1141 catctcgtcg cgctcggcga cgctgagcgc cttgttcacg ccgcccggaa tggcgccggt
     1201 gccatgcacg cgcttgccgg caacgtggcg gatcacttcc tggccatact tgcgcagcag
     1261 cacgccctgc ttggcgatct ccgggaaatc gagggcgacg ccgacgatgt tgcgacgggt
     1321 gaaatcgctg tcgaaaccga gcagcaggtc gggcgacgcg aggtggaaga aatgcaccgc
     1381 gtgcgactgc acgatctggc cgtaatgcag cagccggcgc agcttctcgg cggtcggcgt
     1441 gagcgggccg tagccggcga tctggtccat cgccttgacc gcggcgagct ggtggctgac
     1501 cgggcagatg ccgcacaggc gctgcacggt gaccggcact tcccagtagg gacggccttc
     1561 gatgaagact tcgaagccgc ggaactcgac gatgtgcagg cgtacctggt gaatcttgtt
     1621 gtcctggtcg agcagcaggg tgaccttgcc atggccttcg acccgagtca ccgggtcgat
     1681 cgcgacccgg cgcagggttt cgggatgggc ggcggtttcc agttcaaaca tgaggtactc
     1741 cgatcaatcg aatttgagca gcgggtgctc gatgcgcggc gtgcggccgg tcagcaggtc
     1801 ggtaaggaat ttccagatgg tgtcggcgga cggcggacaa ccgggcagga agtagtcgac
     1861 gcgaaccact tcgttgaccg gatagacctt gtcgagcagc agcggcagtt ccggatcgtc
     1921 gggaatcgcc gtggcgctgc cgtccggcgt gcggtacacc tggctcaggc acttgccgac
     1981 gtcgaggtga ttgcgctgcg ccggcagtcc gccattgacc gcgcaggcgc cgaaggcgac
     2041 caggatcttg gaattggcgc gcagctcgcg caacacatgg acgttctcgg cgttgcagac
     2101 gccgccttcg accagcgcca gatcgcaatg ctggctgcag tgcttgatgt cggtgatcgg
     2161 agagaggtcg aactcgacca gcgcggcgag ttcgagcagg cgttcgtcga tgtcgaggaa
     2221 ggacatgtga caaccgaagc aaccggacag cgaggtggtg gtgacgcgca gcttgcgcgg
     2281 attcatcggt gcgttcattt cagcgctcct cggtttcggc cgccggcacg atggcgatgt
     2341 gccggcgggc caggtaatcg acctgacggc tgatcggctg ctcgtcgaac tggcgttcgc
     2401 cgatcggtac cgcgtagccg acgcgcttga ccaggatggc gccgaccggg cagacgtggg
     2461 cggcggcatc gtcgatgctc agcgcgctgt cgccgagccg gccgctcggc gaattgacga
     2521 cgatgtgcga ttcggcaccg cgcccggcga gcatgaacac gttcttgccg tcgatgtcgc
     2581 ggctggcgcg cacgcacagt tcgcacatga tgcagcggtt gagatcgagc cagacgtcgg
     2641 gatgggtggt atcgatcggc cgcaccgggt aagcgtggcg gaagtgcggc gaaaccattt
     2701 ccagatcgta ggcgagcgcc tgcaactggc agttgccgct cttctcgcag cccgggcaga
     2761 agtggttgcc ctcgacgaag agcatctgca gcagcacccg gcgcctgtcg ttcaaggtcg
     2821 gcgtgttgtt ctcgacctcg atgccgggca tcgctttctg cgtacaggcc gagccgttgc
     2881 gcccgttgac gatcaccgtg cacagcttgc aactgccgtg cggcttgagg tccgggtgat
     2941 gacagaggtg cgggatatag acgccggcgg cgagggccgc ctgcaggatg gtctggccgg
     3001 gcgtgaaggg aatgcgctgg ccgtcgatgg aaaagctgtt ggcgttgctg gtgtcgctca
     3061 tgcttcttct cccaggtggg cggcggcatc gtcgcggccg gcgagttcgc gggcgcggga
     3121 cagggcggcg tcgaggtcga aggccgggac gaagtcggtt tcggccagcc ggcgctcgta
     3181 ggcgggacgg aatttctgca cggtgtcggc gaccgcgttg ccggcggtct ggccgaggcc
     3241 gcagtggctg cccggtttca ggacctgctg caggcgcagc agctcgttca tttcgtactg
     3301 cgtgccgcgg ccgtcggcga tcttgtccat gatctccttc atcagcaccg tgccgacccg
     3361 gcacggcgtg cagaagccgc aggattcgtg agcgaagaag tgggcgaagt tgcgcgccac
     3421 ctcgaacagg tcgcgctgct tgccgaagat catgaacgag ccggcggtcg gcacatcctc
     3481 gaaggcgatc cggcggccga actcgcgttc cgacaaacaa acgccagagg cgccgctgac
     3541 ctgcacggca atcgcgtcct gggcaccgca gtcttccagt acctgggcga tgcgtacgcc
     3601 gaaggcgtat tcgtaaagcc cgggccgcgc gcaatcgccg gcaaccgaca ggatcttggt
     3661 accggtcgat tgcttggtgc cgatcgcttc gtaccaggtg ccaccatgca gggcgatctc
     3721 ggtgaccttg cacagggtct cgacgttgtt gaccacggtc ggcttgccca tgtagccgga
     3781 ggtcaccggg aagggcgggc ggacacgcgg gcggccggcc ttgccttcga gcgattcga
//
LOCUS       DAOSDX010000176         3833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8397_length_3833_cov_2.935945, whole genome shotgun sequence.
ACCESSION   DAOSDX010000176 DAOSDX010000000
VERSION     DAOSDX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8397_length_3833_cov_2.935945"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PLN02_05040"
     CDS             <1..1055
                     /locus_tag="PLN02_05040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI51720.1"
                     /translation="QRAFQNLLIAYLVTQLLLFFLVSASRREWQNQLAGQTARIGELL
                     HQNEVLLHTAGEGIFGVDREQVVTFINPAAQKMLGYAQDAVIGQRLHALIHLRGPDGQ
                     PCEEAACPVALTLGDGQTRTQEEWLRRSDGGIFPVRMTVSPIREGLGYGGAVVVFHDI
                     GELKEKEAALTRLARTDTLTGLNNRGYFLELLGIEISRWQRNGHHVALLMIDLDCFKR
                     VNDTWGHAVGDEVLRHFSEVLRRSLRRVDIAGRLGGEEFAVLLPGDNEVAALAAAERL
                     RQNVANEAASTSAGAITVTISIGVTLFRAGDSSHEQPLRRADQALYRAKQAGRNRVEA
                     DFAAELKPGDAAPADR"
     gene            complement(1021..1848)
                     /gene="nifM"
                     /locus_tag="PLN02_05045"
     CDS             complement(1021..1848)
                     /gene="nifM"
                     /locus_tag="PLN02_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifM"
                     /protein_id="HOI51721.1"
                     /translation="MLHAYLELKLSWELFQKGPETLSDPERNRLSEVATRQDGIEQRI
                     LASPQATHVMVPGPTLQTRIAEIRGRYPDAEAFAQDLERIGLSEAELEKAVARDLRVE
                     AVLDRVAAAEAPVSEVDAEIYYRLHQDAFDRPEARRLRHILITYNSPQQAAEARKTLE
                     KLRTTAHEREKFAAAALRHSQCPTALEGGLLGVVRRKQLFPELEAAAFALAEDGISSV
                     LESEVGLHILRCDEILPSGLLPFAEVRERIIERLTDKRRREAQKRWISGLLAQRRQA"
     gene            complement(1853..2308)
                     /locus_tag="PLN02_05050"
     CDS             complement(1853..2308)
                     /locus_tag="PLN02_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HOI51722.1"
                     /translation="MRPRWEYGDTVRLIRNVRNDGTYPGLDPGEPLVRRGSTGVVVDV
                     GTFLQDQIIYSVNFLELGKIVGCREEELIDIDETWTPSRFEFREKVRSARQLAVDGKV
                     LVELGAIGEVIKVIRDAPGGVAYHIHFDSLPGRLLQIPEATLEPLVDGE"
     gene            complement(2305..2646)
                     /locus_tag="PLN02_05055"
     CDS             complement(2305..2646)
                     /locus_tag="PLN02_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase-stabilizing/protective protein NifW"
                     /protein_id="HOI51723.1"
                     /translation="MEDTLTLAEAMEDLVSAEDFLDYFEIPYDASVVHVNRLHILQRF
                     HDYLARQAPNLPPEEEQQRAIYRLWLERAYLDFTVSDALTEKVFAVFQHVATPEGGMS
                     SFVSLDKVFRQ"
     gene            complement(2656..3195)
                     /locus_tag="PLN02_05060"
     CDS             complement(2656..3195)
                     /locus_tag="PLN02_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51724.1"
                     /translation="MDLLDFADDTLYFEEPLAAEAAALLVQAASEYGEPAAELSLLRA
                     HLLAPDSLTVLVGIYRYYFYQHRLEDALQVAELAMQLSGRRLGLPAWERIDETRLAAA
                     AASSFGMLRFYLLALKAASVVLLRLGRIADSRQRLCKLAALDSRDQLGAGKLLEVVDA
                     FRGNTEDEENIPDLKLVAA"
     gene            complement(3197..>3833)
                     /gene="cysE"
                     /locus_tag="PLN02_05065"
     CDS             complement(3197..>3833)
                     /gene="cysE"
                     /locus_tag="PLN02_05065"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOI51725.1"
                     /translation="YPGLHAILAYRVAHRLWHGGWRYAARFLSFFARMLTNVDIHPGA
                     TIGRRFFIDHGACVVIGETAEIGDDVTLYHGVTLGGTSWRKGKRHPTLENGVVVGAGA
                     KILGPITIGERVRVGANSVVVKNVPADRTVVGIPGRIVDTRAARKPGSIDLEHNVLPD
                     PVARSIACLIERIETLEKELATLRHEPAPHTASDCQTCSAGDLCCESEAMH"
BASE COUNT          600 a         1193 c         1360 g          680 t
ORIGIN      
        1 agcagcgagc cttccagaac ctgttgatcg cctatctcgt gacgcagttg ctgctgtttt
       61 tcctggtcag cgcctcgcgc cgcgaatggc agaaccagct cgccgggcag acggcgcgca
      121 tcggcgaatt gctgcatcag aacgaggtgc tgctgcacac cgccggcgag ggcattttcg
      181 gggtcgatcg cgaacaggtg gtcaccttca tcaatccggc ggcgcagaag atgctgggct
      241 atgcgcagga cgccgtgatc ggccagcgcc tgcacgcgct gattcatctg cgcggtccgg
      301 atgggcagcc ttgcgaggag gcggcctgtc cggtggcgct gaccctgggc gacggccaga
      361 cgcggaccca ggaggaatgg ctgcgccgca gcgacggcgg catcttcccg gtgcgcatga
      421 cggtgtcgcc gatccgcgaa ggtctgggct atggcggtgc cgtcgtcgtt tttcacgaca
      481 tcggcgaact caaggagaag gaagccgcat tgacccggct ggcccggacc gacacgctga
      541 ccggactgaa caaccgtggt tatttcctgg agctgctcgg catcgagatc agtcgctggc
      601 agcgcaatgg acaccacgtg gccttgttga tgatcgacct cgactgcttc aagcgggtca
      661 acgacacctg gggccacgcc gtcggcgacg aggtgctgcg ccatttttcc gaggttctgc
      721 gccgttcgct gcgccgcgtc gatatcgcgg gtcgcctggg gggcgaggag ttcgcggtgc
      781 ttttgcccgg tgacaacgag gtggccgcgc ttgccgccgc cgagcgcctg cgtcagaacg
      841 tcgccaacga ggcggcgtct accagtgccg gcgccatcac ggtcaccatc agcatcggcg
      901 tcaccctttt ccgggccggc gacagcagtc acgagcagcc gctgcggcgc gccgaccagg
      961 cgctttaccg ggccaagcag gccggccgca accgggtcga agccgacttc gcggccgaac
     1021 tcaagcctgg cgacgctgcg ccagcagacc gctgatccag cgtttctgcg cttcccggcg
     1081 gcgtttgtcg gtgaggcgtt cgatgatgcg ctcgcgcacc tcggcgaagg gtagcaggcc
     1141 gctgggcagg atttcgtcgc aacgcaggat gtgcaggccg acttccgatt cgagcacgct
     1201 gctgatgcca tcctcggcca gggcaaaggc cgccgcttcg agctccggga acagttgctt
     1261 gcggcggacg acgccgagca gtccgccttc gagcgcggtc ggacactgcg agtggcgcag
     1321 cgcggcggcg gcgaatttct cgcgctcgtg cgccgtcgtg cgtagctttt ccagcgtctt
     1381 gcgggcctcg gcggcctgtt gcggggaatt gtaggtgatc aggatgtggc gcaggcggcg
     1441 cgcttccggc cggtcgaagg cgtcctggtg gaggcggtag tagatttccg cgtcgacctc
     1501 gctgaccggc gcttcggcgg cggcgacgcg gtcgagcacg gcttcgacgc gcaggtcgcg
     1561 ggcgaccgcc ttctccagct cggcttcgct gagcccgatg cgctcgagat cctgggcgaa
     1621 agcctcggca tccgggtagc ggccgcgaat ctcggcgatc cgggtctgca gcgtcggtcc
     1681 cggcaccatc acgtgggttg cctgcggact ggccaggatg cgctgttcga tgccgtcctg
     1741 gcgggttgcc acttcgctga ggcggttgcg ctccgggtcg ctgagcgttt ccggcccctt
     1801 ttgaaacagt tcccaggaaa gtttcagttc gagataggca tgcaacatgg tcttactccc
     1861 cgtcgacaag gggttccagg gtagcttccg ggatttgtag caggcgcccc ggcagactgt
     1921 cgaaatggat gtgataggca acgccgcccg gcgcgtcgcg gatcaccttg atgacttcgc
     1981 cgatcgcccc gagttcgacc aggaccttgc cgtccacggc gagctggcgg gcgctgcgta
     2041 ccttctcgcg gaattcgaaa cgcgacggcg tccaggtttc gtcgatgtcg atcagctctt
     2101 cctcgcgaca gccgacgatc ttgcccagtt cgagaaagtt caccgagtag atgatctgat
     2161 cctgcaggaa ggtgccgaca tcgacgacga cgccggtgct gccgcggcgc accagcggct
     2221 cgccggggtc gagcccaggg taggtgccgt cgttgcgcac gttgcggatc aggcgcacgg
     2281 tgtcgccata ttcccagcgt ggtctcattg tcggaagacc ttgtccagcg agacgaagga
     2341 actcatgcca ccttccggcg tggcgacgtg ctggaagacg gcgaagacct tctcggtcag
     2401 cgcgtcggag acggtgaagt cgagataggc gcgttccagc cagaggcggt agatggcgcg
     2461 ctgctgttcc tcttccggcg gcaggttggg cgcctgacgg gccaggtagt cgtggaagcg
     2521 ctgcaggatg tgcaggcggt tcacatggac caccgaggcg tcgtagggga tttcgaaata
     2581 gtccaggaag tcttcggcgg agaccaggtc ttccatcgct tcggctaggg tcagggtgtc
     2641 ttccatgatg cctcctcagg ctgcaacaag tttcaaatcg ggaatgttct cttcgtcttc
     2701 cgtgttgcca cggaaggcgt cgaccacttc gagcagcttg ccggcgccga gctggtcgcg
     2761 gctgtcgagc gcggccaact tgcacaggcg ctggcgggaa tcggcgatgc ggccgagacg
     2821 aagcaggacg acgctggccg ccttcagcgc cagcaggtag aagcgcagca tgccgaatga
     2881 gctggcggcg gcggcggcca gccgcgtttc gtcgatgcgc tcccaggccg gcaggccgag
     2941 ccgacggccg gagagctgca tggcgagttc ggcgacctgc agcgcgtctt ccagacggtg
     3001 ctggtagaag tagtagcggt agatgccgac cagcacggtc aggctgtccg gagccagcag
     3061 gtgggcgcgc agcagcgaca gttcggcggc cggttcaccg tattcgctgg ccgcctggac
     3121 aaggagcgcg gcggcttcag ccgccagcgg ctcctcgaag tacagcgtgt cgtcggcgaa
     3181 atcgagcagg tccatgtcag tgcatcgcct cgctctcgca gcacaggtcg ccggcgctgc
     3241 aggtctggca gtcgctggcg gtgtgcggcg ccggttcgtg gcgcagcgtc gccagttcct
     3301 tttccagcgt ctcgatgcgt tcgatcaggc aggcgatcga gcgtgccacc gggtcgggca
     3361 ggacgttgtg ttcgaggtcg atgctgcccg gcttgcgcgc ggcgcgggta tcgacgatgc
     3421 ggccgggaat gccgaccacg gtgcggtcgg ccgggacgtt cttgaccacc accgagttgg
     3481 caccgacgcg gacccgttcg ccgatcgtga tcggcccgag aatcttggcg ccggcgccga
     3541 cgacgacacc gttctccagc gtcgggtggc gtttgccctt gcgccacgag gtgccgccca
     3601 gcgtgacccc gtggtagagg gtgacgtcgt cgccgatctc ggcggtctcg ccgatcacga
     3661 cgcaggcgcc gtgatcgatg aagaagcggc ggccgatcgt cgcccctggg tggatgtcga
     3721 cgttggtcag catgcgggcg aagaaggaga ggaagcgcgc cgcgtaacgc cagccgccgt
     3781 gccacaggcg gtgggcgacg cggtaggcga ggatggcgtg caggccggga tag
//
LOCUS       DAOSDX010000177         3785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8537_length_3785_cov_3.290080, whole genome shotgun sequence.
ACCESSION   DAOSDX010000177 DAOSDX010000000
VERSION     DAOSDX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8537_length_3785_cov_3.290080"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLN02_05070"
     CDS             <1..521
                     /locus_tag="PLN02_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HOI51726.1"
                     /translation="RDWPLHTPWFREIPREFVHFLAQTPGLRLPRWLPELAHYEWVEL
                     AADIADAPIPPHDPDGDLRHGTVVLNPVLFNLAYAWPVHRIGPDWRPRRPAATHLLVH
                     RDADDVVRFSETNPVTARLLALLAATPQSGATAARQIAGELQHGDPERLVDFAVELLV
                     DLRRQGVVLGTL"
     gene            573..1082
                     /locus_tag="PLN02_05075"
     CDS             573..1082
                     /locus_tag="PLN02_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOI51727.1"
                     /translation="MYHTLTALTRRLLAGLDLIGLWIGLLTLRLVLGWEYFEAGQAKF
                     LGENWFADIQDRFPFPFNLVPPELSWQMATWFELIGGIALVLGLATRFFSLSLIVLTV
                     VAILSVHWPDSWQTLGDLLQGYAITDDGHGNYKLPAIFLAMLLPLLLLGPGRLSLDHW
                     LRRRLKRSA"
     gene            1165..1803
                     /locus_tag="PLN02_05080"
     CDS             1165..1803
                     /locus_tag="PLN02_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI51728.1"
                     /translation="MDTPAPLTRKRGRPPKRPEESFETRELLLRAGLEVLTEKGFAAT
                     GIDEVLKRVGVPKGSFYHYFDSKEAFGAALIERYASYFARKLERHFRDESMRPLERIT
                     AFVDDAQAGMARHDFRRGCLIGNLGQEMAALPESFRGRLSAVFDDWEQRLARCLEAAQ
                     RLGQIPDAVDCAQQASFFWIGWEGAVLRAKLERSAKALDIFTQGFFAGLQRS"
     gene            1854..2837
                     /locus_tag="PLN02_05085"
     CDS             1854..2837
                     /locus_tag="PLN02_05085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HOI51729.1"
                     /translation="MFKGILIEKDDAGYRANLKEIDEAQLPEGDVTVRVSHSTLNYKD
                     GLAITGKGPVVRRFPMVPGIDLVGTVESSTHVDYQPGDSVLLNGWGVGEGHWGGLAQK
                     ARLKGDWLVPLPKQFSPLQAMAIGTAGYTAMLCVLALERHGVKPEHGEIVVSGAAGGV
                     GSVAITVLAKLGYTVVAVSGRSEEADYLKRLGASEVLNRADFSSPGKPLGKERWAGAV
                     DVVGSHTLANICATTKYRGVVTACGLAGGMDFPATVAPFILRGVTLAGIDSVMCPRPE
                     RLEAWRRLGTDLDISKLEMIGTEIALSEAIPHAFKLINGEIRGRVIVDVNR"
     gene            2935..3315
                     /locus_tag="PLN02_05090"
     CDS             2935..3315
                     /locus_tag="PLN02_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOI51730.1"
                     /translation="MKTIRLLAALAFTTLAGFAQALDIAPWSPDDFARQQAAGHAVAL
                     HFHADWCPTCRAQQQVFNGWRGDASVPGRLLVVDYDNARELRRQFGVRTQSTLIVFKG
                     SKEVARLAGETDPAKLRAALEAAR"
     gene            3312..>3785
                     /locus_tag="PLN02_05095"
     CDS             3312..>3785
                     /locus_tag="PLN02_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOI51731.1"
                     /translation="MSYHGGIPARAMEAAIDPSAVIDNAFVEQVRHDMLAFAQLQLRD
                     ATLAEDAVQEALTAALTGGRDFAGRSALKTWVFGILRHKIVDLIRQQSRTTPISAFGG
                     DDDSLDQAFDTLFKENAHWHPAHRPATWGDPEASLRGKHFWAVFDACLTHLPKNTA"
BASE COUNT          653 a         1333 c         1161 g          638 t
ORIGIN      
        1 tgcgtgactg gccgctgcat acgccgtggt tccgcgaaat cccgcgcgag ttcgtgcact
       61 tcctggcgca gacgccgggc ctgcgtctgc cgcgctggct gccggaactg gcgcattacg
      121 agtgggtgga actggccgcc gacatcgccg acgcgcccat cccgccgcac gatccggacg
      181 gcgacctgcg ccacggcaca gtcgtcctca acccggtgct gttcaatctt gcctacgcct
      241 ggccggtgca ccgcatcggc cccgactggc ggccgcgccg gccagcggca acgcatctgc
      301 tggtccatcg cgacgccgac gatgtcgtcc gcttcagcga gaccaacccg gtcaccgccc
      361 gcctgctcgc gctgcttgcc gcaacaccgc agagcggcgc aaccgccgcg cggcagatcg
      421 ctggcgaact gcaacacggc gaccccgagc ggctggtcga tttcgccgtc gaactgctcg
      481 tcgacctgcg ccgacaaggc gtcgtgctcg gcacactgta agtttctgcg cccccgctcc
      541 gaccaaggat caaaccccta cggagcctga ccatgtacca caccctcacc gccctcaccc
      601 gtcgcctgct cgccggcctc gacctgatcg gcctgtggat cggcctgctg acgctgcgcc
      661 tcgtgctcgg ctgggaatac ttcgaagccg ggcaggccaa gttcctcggc gaaaactggt
      721 ttgccgacat ccaggaccgc tttcccttcc cgttcaacct cgtgccgccc gaactgagct
      781 ggcagatggc cacctggttc gagctgatcg gcggcatcgc gctggtcctc ggcctggcca
      841 cgcgcttctt ctcgctgtcg ctgatcgtcc ttaccgtcgt cgccattctc agcgtgcact
      901 ggccggatag ctggcagacg ctgggcgacc tgctgcaagg ctacgcaatc accgacgacg
      961 gccacggcaa ctacaagctg ccggcgatct tcctcgccat gctgttgccg ctcctcctgc
     1021 tcggccccgg ccgcctgtcg ctcgaccact ggctgcgccg ccgcctcaaa cgcagcgcct
     1081 gaagcatcgc ccatcaaagc ccggtcaggg tcgctggaat ccgcttgttg catttattga
     1141 gacgaccggt ctaaaattcg gcgcatggat accccagcgc cccttacccg caaacgcggc
     1201 cgcccgccaa agcggccaga agaaagcttc gaaacgcgcg aactgctgct gcgcgccggg
     1261 ctggaggtgc ttaccgaaaa gggatttgcg gccaccggga tcgacgaagt actgaaacgg
     1321 gtcggcgtgc cgaagggctc cttctatcac tatttcgaca gcaaggaagc tttcggggcg
     1381 gcgttgatcg agcgttacgc gagctacttt gcccgcaagc tggagcgcca cttcagggac
     1441 gaatcaatga ggccgcttga gcgcatcaca gccttcgtcg acgacgcgca agccggcatg
     1501 gcgcggcacg atttccgccg tggctgcctg atcggcaatc tcggccagga gatggccgcc
     1561 ttgccggaaa gcttccgtgg ccgtctcagc gccgtatttg acgactggga gcagcgtctg
     1621 gcccgttgtc tggaagccgc gcaacgactc ggccagattc ctgacgcggt ggattgcgcg
     1681 cagcaagcca gttttttctg gatcggctgg gaaggggcgg tcttgcgcgc caaactcgaa
     1741 cgcagcgcca aagcgctcga catctttacg caaggatttt tcgccgggct acagcggtcc
     1801 tgatttcttt gactttttat agacgatcgg tctaaatcac caaggagatg aacatgttca
     1861 aaggcattct gatcgagaag gatgacgccg gctatcgcgc caatctcaag gagatcgacg
     1921 aagcacagtt gcccgagggc gatgtcaccg tccgcgtcag ccactcgacg ctcaactaca
     1981 aggacggtct ggccattacc ggcaaggggc cggtggttcg acgcttcccg atggtgccgg
     2041 ggatcgatct ggtcggcacc gttgaatcga gcactcacgt cgactaccag ccgggcgaca
     2101 gcgtgctgct caacggctgg ggcgtcggcg aaggacactg gggcgggctg gcccagaagg
     2161 cgcggctcaa gggcgactgg ctggtgccgc tgcccaagca gttttccccg ctgcaggcca
     2221 tggcaatcgg caccgccggc tacaccgcca tgctctgcgt gctggcgctg gagcgccacg
     2281 gggtgaagcc ggagcacgga gaaatcgtcg tttccggcgc cgccggtggc gttggcagcg
     2341 tggcaatcac ggttttggcg aaactcggtt atacggtggt cgcggtcagc ggccggagcg
     2401 aggaagccga ctacctgaaa cgcctgggcg ccagcgaagt gctcaatcgg gccgatttct
     2461 cgtcgccggg caagccgctg ggcaaggagc gctgggccgg tgcggtcgac gtcgtcggca
     2521 gccacaccct ggccaacatc tgcgccacca ccaagtatcg cggcgtggtg acggcatgcg
     2581 gactggccgg cgggatggat ttcccggcaa cagtggcgcc gttcatcctg cgcggcgtga
     2641 cgctggccgg catcgacagc gtgatgtgcc cgcgccccga gcgtctcgag gcctggcgtc
     2701 gcctgggcac cgacctcgac atctccaagc tggagatgat cggcaccgag attgcgctga
     2761 gcgaggcgat tccccacgcc ttcaagctca tcaacggcga gattcgcggc cgggtgatcg
     2821 tagacgtcaa tcgctgacaa agagcggccg gtccggcaaa ggctggcagc agtgtaagaa
     2881 ttcaccccct ggttccgacc aatcatcacc atcctcaagc catcggagcc catcgtgaaa
     2941 accattcgtc tgctcgccgc ccttgccttc accaccctcg ccggttttgc ccaggccctc
     3001 gacatcgccc cctggagccc ggacgatttc gcccgccagc aggcggccgg ccacgccgtc
     3061 gcgctgcact tccacgccga ctggtgcccg acctgccgcg cccagcaaca ggtcttcaac
     3121 ggctggcgcg gcgacgccag cgtgcccggc cgcctgctcg tcgtcgatta cgacaacgcc
     3181 cgcgaactgc gccgccagtt cggcgtgcgc acgcaatcga cgctgatcgt cttcaagggc
     3241 agcaaggaag tcgcccgtct ggccggtgag accgacccgg ccaaactgcg cgccgcactg
     3301 gaagccgcgc gttgagctac catggcggca tccctgcccg tgccatggag gccgccatcg
     3361 acccgtctgc cgtcatcgac aacgccttcg tcgaacaggt ccgccacgac atgctggcct
     3421 tcgcccagct ccagttgcgc gacgccacgc tcgccgaaga tgccgtccag gaagcgctga
     3481 ccgccgcgct caccggcggc cgcgactttg ccgggcgctc ggcgctgaag acctgggtct
     3541 tcggcatcct gcgccacaag atcgtcgacc tgatccgcca gcaatcccgg acgacgccga
     3601 tctctgcctt cggcggcgat gacgacagcc tcgaccaggc cttcgacacc ctgttcaagg
     3661 aaaacgccca ctggcacccg gcgcaccgcc cagccacctg gggcgacccg gaagcctcgc
     3721 tgcgcggcaa acacttctgg gccgtcttcg acgcctgcct gacccacctg ccgaagaaca
     3781 ccgcc
//
LOCUS       DAOSDX010000178         3781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8548_length_3781_cov_3.409554, whole genome shotgun sequence.
ACCESSION   DAOSDX010000178 DAOSDX010000000
VERSION     DAOSDX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8548_length_3781_cov_3.409554"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1285)
                     /locus_tag="PLN02_05100"
     CDS             complement(116..1285)
                     /locus_tag="PLN02_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOI51732.1"
                     /translation="MNERQLILLIAGCLMLQPLSTDLYLASLPRLGEAFSAPKILVQQ
                     TLVFFSVGFGLAQLLGGPLSDRFGRRRVMLGGLSVYLLASLACALAPSIYLLIGARFA
                     QACGCCAAIVAARALIRDLYPPAIGAAKLAQASSYMAFAVVSGPVIGAQLQVHFGWQA
                     AFVFLSCVVPLLLALAWRWQEAPLSGASGQAAGYRSLLTHPVFRSYALAGTLSYSNIF
                     VFISGSSFVLIELLGVSTANYGYCFASGCAGYLAGAFLCRRLLAVLGTERTLLAGSTV
                     MVLAAATFLACCLAGVMNWWLLVAFQCLSMFSHGIIFPCAQIGSLAPFPGSAGRAAGL
                     FGAVSMIGALTISNLVGLLLHDSVLPLALLAASTGLLQWLLLRRGQRKGTLRLEH"
     gene            1553..2587
                     /locus_tag="PLN02_05105"
     CDS             1553..2587
                     /locus_tag="PLN02_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOI51733.1"
                     /translation="MKAKHLLALAALSTLIPGAVLAQEVVLKVAHFVPPTAPGHTKFI
                     APWCDKLASESAGRIKCQIYPAMQLGGTPAQLLSQVRDGVADVVWTLPGYTPGRFPVT
                     EVFELPFMTREQEGSSRALWDFVQKNTLKSEFAGLHPLATWVPGPYTLHLRAAQPKVM
                     ADLKDMKIRSSSRLTTRLLSALGASPVGMPVTQMSEALSKGVIEATMLPWEVVPAMKV
                     HELTKFSAEFGGDHTLTTTTMVFVMNKKKYDSLPADLKKVIDANSGRETSAWVSGQLK
                     SADAASRAATLKNGNTIYQIPAEETAKWKKTASTVNDEWVKEVSAKGVDGNKLLAEAN
                     ALVDHYADKK"
     gene            2654..3172
                     /locus_tag="PLN02_05110"
     CDS             2654..3172
                     /locus_tag="PLN02_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOI51734.1"
                     /translation="MAEVMDEPLARQSGAVGWVIEKLCQAMAIMAGAAFVAEMLLSAV
                     SVMLRALIGRGVAGDFELVQVLSAMGIALCLPYCQLQKGHVFVDFFTLWAPALLKRFL
                     DAIAALLLAACSFFLAWRIWEGMADLREYGEATMVLNLPIWWGYIPVVPAFVMLGVTA
                     LYTAYGELRGRA"
     gene            3172..>3781
                     /locus_tag="PLN02_05115"
     CDS             3172..>3781
                     /locus_tag="PLN02_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOI51735.1"
                     /translation="MSSTTIGLIMGGVMMFFMAARIPVGITMFLTGAIGYVWVSGLDP
                     LLGFLKNAPYGRFSLYDLSVVPLFLLMGQFATHGGLSRSLFKAGNAFIGHWRGGMAMA
                     GTASCAGFGAICGSSLATAATMAHVVLPELRHHQYKNSLAAGSLAAGGTLGILIPPSV
                     PLVIYGILAEQNIAKLFLAAFVPGVLAAIGYMVVISIVAHLNP"
BASE COUNT          709 a         1132 c         1270 g          670 t
ORIGIN      
        1 gatagagtac tgccctccga aggcaggggt cgtgcgttcg aatcgcgccg ggcgggccag
       61 aacatccaat gggttaagtc tttccgactt aacccatttt tgtttgctga ctgccctaat
      121 gctccaggcg cagtgtcccc ttgcgttggc cgcggcgcag cagcaaccac tgcaggaggc
      181 cggtgctggc ggcgagcagg gcgagcggca gcacgctgtc gtgcagcagg aggccgacca
      241 ggttgctgat ggtcagcgcg ccgatcatgc tgaccgcccc gaacagcccg gccgcgcgtc
      301 cggcggagcc gggaaagggc gccagcgagc cgatctgggc gcaagggaag atgatgccgt
      361 gcgagaacat cgacaggcat tgaaaggcga ccagcaacca ccagttcatc actcccgcca
      421 ggcagcaggc gaggaaggtc gcagcggcca gcaccatcac cgtgctgccg gcaagcaagg
      481 ttctttccgt gcccagcact gcgagcagac ggcggcagag gaaggcaccg gcgagatagc
      541 cggcgcagcc gctggcgaag caatagccgt aattggcggt cgatacgccg agcagctcga
      601 tcagcacgaa tgacgagccg gagatgaaga cgaagatgtt gctgtagctc agggtgccgg
      661 ccagcgcata gctgcggaag accgggtggg tgagcaggct gcgatagccg gcggcctgcc
      721 cgctggcgcc ggagagtggc gcttcctgcc agcgccaggc cagcgccagg agcaagggaa
      781 cgacgcagga gaggaagacg aaggcggcct gccagccgaa atggacttgc aactgggcgc
      841 caatcaccgg tcccgagacg acggcgaaag ccatgtagct cgatgcctgg gccagcttgg
      901 cggcgccgat ggccggcggg tagaggtcgc ggatcagggc gcgcgcggcg acgatcgcgg
      961 cgcagcagcc gcaggcctgg gcgaagcggg cgccgatcag cagatagatg ctcggtgcca
     1021 gggcgcaggc caggctggcg agcaggtaga cgctcaatcc gccgagcatg acgcgccgtc
     1081 gaccgaagcg gtcggacagg gggccgccga gcaactgggc gagaccgaag ccgaccgaga
     1141 agaagaccag ggtctgctgg accaggattt tcggtgccga gaaggcctcg ccaagccgcg
     1201 gcagcgacgc cagatagagg tcggtcgata gcggctgcag catcaggcag ccggcgatca
     1261 ggaggatcaa ctggcgttcg ttcacgaaga tttccggaaa ttggtccgcg ccaactctat
     1321 ccgtcgccgg tgatttgccc tatgccataa accgactagg tgctattccg catttgacta
     1381 actgcttccg tcgatcatgg aatggtgaat aagtatcgca atattcaata cctgatatgg
     1441 ctttgcctcc ctaggcgggg gcgggcggca ttgacgacaa tccggagcgc taatgggcat
     1501 tctagcggtg tgtttttagc gactcgataa cactgactta cggagacaaa acatgaaagc
     1561 caaacatctg ctggcgcttg ccgccctgtc cacgctgatt cccggggccg tcctggccca
     1621 ggaagttgtc ctcaaggttg cccatttcgt gccgccgacg gcgccgggtc ataccaaatt
     1681 catcgctccc tggtgcgaca agctggccag cgaatcggcc ggccgcatca agtgccagat
     1741 ctatcccgcg atgcaactgg gtggaacgcc ggcacaactg ctgtcgcagg tccgcgacgg
     1801 tgttgccgac gtggtgtgga cgctgccggg atataccccc ggtcgtttcc cggtgaccga
     1861 ggtgttcgaa ctgccgttca tgacccgcga gcaggaaggc tcctcgcgcg cgctctggga
     1921 tttcgtgcag aagaacaccc tgaaaagcga atttgccggt ctccacccgc tggcgacctg
     1981 ggtgcccggc ccttataccc tgcacctgcg ggctgcccag ccgaaagtca tggccgacct
     2041 caaggacatg aagatccgca gctcttcccg tctcaccacc cgtcttttgt cggcgctcgg
     2101 cgccagtccg gtcggcatgc cggtgacgca gatgtccgag gccctgtcca agggggtgat
     2161 cgaagcgacc atgctgccct gggaagtggt gccggcgatg aaggtgcatg agctgaccaa
     2221 gttctccgcc gagttcggcg gcgaccacac gctgacgacg acgaccatgg ttttcgtcat
     2281 gaacaagaag aaatacgaca gcctgcccgc cgacctgaaa aaggtgatcg acgccaacag
     2341 cggccgcgag acctcggcct gggtcagtgg ccagttgaag agcgccgacg ccgccagccg
     2401 cgccgcgacc ctgaagaacg gcaacacgat ctaccagatt ccggcggagg agaccgccaa
     2461 gtggaagaag actgccagca cggtcaacga cgaatgggtc aaggaggttt cggccaaggg
     2521 cgtcgatggc aacaagctgc tcgccgaggc caacgccctg gtcgatcact acgccgataa
     2581 aaaataatcc gcggatgggg ccggcggcag gtctgtcgcc ggcccgcagt cggtgacgga
     2641 agcggagagc gagatggctg aagtaatgga tgaacccctg gcgcgccagt cgggcgccgt
     2701 agggtgggtg atagagaagc tgtgtcaggc gatggcgatc atggccgggg cggccttcgt
     2761 ggccgagatg ctgctatcgg cggtgagcgt gatgttgcgc gccttgatcg ggcgtggtgt
     2821 cgcgggagat tttgaactgg tccaggtgct gtcggcaatg gggatcgcct tgtgtcttcc
     2881 ctattgtcag ctgcagaagg ggcacgtctt cgtcgatttc tttaccttgt gggcgcccgc
     2941 gctactcaag cgttttctgg atgccattgc cgccttgctg ctggcggcct gttccttctt
     3001 cctggcctgg cgcatctggg aagggatggc tgacttgcgt gaatacggcg aggcgacgat
     3061 ggtgttgaac ctgccgatct ggtggggcta catcccggtg gtgccggctt tcgtgatgct
     3121 tggtgtcaca gcgctgtaca ccgcatatgg tgaattgcgg gggagagcat gatgagtagt
     3181 acgacaatcg gcctgatcat gggcggcgtc atgatgttct tcatggcggc gcgtatcccg
     3241 gtcgggatca cgatgttcct gaccggggcc atcggctatg tctgggtgtc cggactcgac
     3301 ccgctgctcg gcttcctgaa aaatgctccc tacgggcgct tttcgctgta cgacctgtcg
     3361 gtggtgccgc tgttcctgct gatgggccag ttcgccaccc acggcggcct gtcgcgttcc
     3421 ctgttcaagg ccggcaacgc tttcatcggc cactggcgcg gtggcatggc gatggccggg
     3481 acggcctcct gcgccggctt cggcgcgatc tgcggttcct cgctggcaac ggcggcgacg
     3541 atggcccatg tcgtgctgcc ggaactgcgt caccaccaat acaagaactc cctggctgcc
     3601 ggctcgctgg cggcaggagg gacgctcggc atcctgattc cgccgtcggt accgctggtc
     3661 atctacggca tcctcgccga gcagaacatc gccaagctgt tcctcgccgc cttcgtgccg
     3721 ggggtgctgg ccgcgatcgg ctacatggtg gtgatttcca tcgtcgccca cctgaatccg
     3781 g
//
LOCUS       DAOSDX010000179         3776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8567_length_3776_cov_2.933620, whole genome shotgun sequence.
ACCESSION   DAOSDX010000179 DAOSDX010000000
VERSION     DAOSDX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8567_length_3776_cov_2.933620"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLN02_05120"
     CDS             complement(<1..754)
                     /locus_tag="PLN02_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOI51736.1"
                     /translation="MTPIYLDNNATTRVDPAVVEAMLPFFTEHFGNASSIHAFGSEVG
                     KALKKARGQVQALLGAEHDSEIIFTSCGTESDSTAILSALKAQPERNTVITTVVEHPA
                     ILTLCEWLEKEGYIVHKLKVDKKGRIDMDEYKSLLHDRVAVVSAMWANNETGTIFPVE
                     EMAELAAAKGIMFHTDAVQAVGKIPIDLKNSKIDMLSLSGHKLHAPKGIGVLYLRRGC
                     RFRPLLRGGHQERGRRAGTENSASIVALGMACE"
     gene            complement(813..1730)
                     /gene="nifU"
                     /locus_tag="PLN02_05125"
     CDS             complement(813..1730)
                     /gene="nifU"
                     /locus_tag="PLN02_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOI51737.1"
                     /translation="MWEYSDKVREHFFNPRNSGPLEEANGIGDVGSIQCGDALRLMLK
                     VNPETEVIEDAHFQTFGCGSAIASSSALTEMIKGMTLDEALKVSNQDIADFLDGLPPE
                     KMHCSVMGREALQAAVANYRGEEWSDDHEEGALVCKCFAIDAVMIEDVVKANNLNTVE
                     QVTFYTKAGGGCAACHEGIEEILAKVKAERAGPGEVSPPPGHLPVLQTEAAKPAAAPK
                     PAAKLSLVQKIRKIEEVLEEVRPMLQRDHGDIELADVQGKKIYVHLKGACSGCMMEAA
                     TLGGVQQKMIEALGELVQVLPASLMPAEA"
     gene            complement(1741..2064)
                     /locus_tag="PLN02_05130"
     CDS             complement(1741..2064)
                     /locus_tag="PLN02_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOI51738.1"
                     /translation="MINLTPAATKAVQRFIRGSETPVIGLRLMISGGGCSGLQYGMKL
                     EAEKAEDDWEHEVDGVKLLVDPMTMPMIDNVTIDFVDTLTHTGFKFDNPNASAQCSCG
                     SSFSV"
     gene            complement(2188..2604)
                     /locus_tag="PLN02_05135"
     CDS             complement(2188..2604)
                     /locus_tag="PLN02_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51739.1"
                     /translation="MNLDTRLLLLHKQGTSGRLRYLCLPCGVALFTPLPGAAALRPED
                     WSPTVQPHPGAPLREAEGQLGLAEGAIEAVPDFHCWVDTPAGDVAVLLGGFTSIDPPF
                     AAAERLGGRFITIAEARPLSAIERDLMRRAYEHVLG"
     gene            complement(2601..2942)
                     /locus_tag="PLN02_05140"
     CDS             complement(2601..2942)
                     /locus_tag="PLN02_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51740.1"
                     /translation="MTLPSPEIGESLCQALREQVGKLGLSIGDEPRWSAAGFETVRDP
                     FSQEESVVAVWRGGERFGKATFFPDGRVFAEYQVLLPHPTQSGAYVDAVQVWGRPQAW
                     RGEAVIVEYPA"
     gene            complement(2968..3189)
                     /locus_tag="PLN02_05145"
     CDS             complement(2968..3189)
                     /locus_tag="PLN02_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HOI51741.1"
                     /translation="MSDEELAALDKEVKKLKRIASEWASQMHDLVEDRLPAAYQEIPG
                     IAQSTYDACQAWAEANQRLLAAQKARAAA"
     gene            complement(3200..3649)
                     /locus_tag="PLN02_05150"
     CDS             complement(3200..3649)
                     /locus_tag="PLN02_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HOI51742.1"
                     /translation="MSTDPIFETDFIKEMARQMRALDTYGTYSGWTVEQILDPFIMSK
                     ERKATIPLIGDPDDETISRVKAFYNAIAVLIEKECKLLAVPLVHLTHEGFGRALITVG
                     KLVAVDKTLRDVHRFGFASLSKMKDEADKLLGIALERIGQYPAVAGL"
BASE COUNT          610 a         1205 c         1247 g          714 t
ORIGIN      
        1 gttcgcaggc catgccgagg gcgacgatgg acgccgagtt ctcggtgccg gcccggcggc
       61 cgcgttcctg gtgaccgccg cgcagcagcg gccggaaacg gcagccgcga cgcaggtaga
      121 gaacgccgat gcctttcggc gcgtgcagct tgtggccgga cagcgacagc atgtcgatct
      181 tgctgttctt caggtcgatg ggaatcttgc ccacggcctg caccgcgtcg gtatggaaca
      241 tgatgccctt ggcggcggcg agttcggcca tttcctcgac cgggaagatg gtgccggtct
      301 cgttgttggc ccacatcgcc gagacgacgg cgacgcggtc gtggagcagc gacttgtact
      361 cgtccatgtc gatgcggccc ttcttgtcca ccttcagctt gtggacgatg tagccttcct
      421 tctccagcca ttcgcagagc gtcaggatcg ccgggtgctc gacgacggtg gtgatcaccg
      481 tgttgcgttc cggctgcgcc ttgagcgccg acaggatggc ggtcgagtcg gactcggtgc
      541 cgcaggaggt gaagatgatt tccgagtcgt gctcggcgcc gagcagcgcc tgcacctggc
      601 cgcgcgcttt cttcagcgcc ttgccgactt cgctgccgaa ggcatggatc gacgaggcgt
      661 tgccgaaatg ctcggtgaag aaaggcagca tggcctcgac gacggcgggg tcgacccgcg
      721 tcgtcgcatt gttatccagg tagatcgggg tcatggcgca gtccttgtca gaatttccgt
      781 tggggtcggg ggatcgttgt ctttttttga acttacgctt cggccggcat cagcgaggcg
      841 ggcagtacct gcaccagttc gccgagggcc tcgatcatct tctgctggac gccgcccagg
      901 gtggccgctt ccatcatgca gccggagcag gcgcccttca ggtggacata gatcttcttg
      961 ccctgaacgt cggccagttc gatgtcgccg tggtcgcgct ggagcatcgg gcggacctct
     1021 tccagcactt cttcgatctt gcggatcttc tgcaccagcg acagcttggc ggccggcttc
     1081 ggcgccgcgg cgggcttggc ggcttccgtc tgcagcaccg gcaggtggcc cggcggcggc
     1141 gacacttcgc cggggccggc acgctcggcc ttgaccttgg cgagaatttc ctcgatccct
     1201 tcatggcagg ccgcgcagcc gccgcccgcc ttggtgtaga aggtgacctg ttcgaccgtg
     1261 ttgaggttgt tggccttgac cacgtcctcg atcatcaccg cgtcgatggc gaagcacttg
     1321 cagaccagcg cgccctcttc atggtcgtcc gaccattcct cgccgcggta gttggcgacg
     1381 gcggcctgca gggcttcgcg gcccatcacc gagcagtgca tcttttccgg cggcagaccg
     1441 tcgaggaagt cggcgatgtc ctggttggaa accttgagcg cctcgtccag cgtcatgccc
     1501 ttgatcatct cggtcagtgc cgacgacgaa gcgatggccg agccgcagcc gaaagtctgg
     1561 aagtgcgcgt cctcgatcac ctcggtttcc gggttgacct tgagcatcag gcgcagcgca
     1621 tcgccgcact ggatcgagcc gacgtcgccg atgccgttgg cttcttccag cggaccggag
     1681 ttgcgcggat tgaagaagtg ctcgcggacc ttgtcagaat attcccacat gattgtttcc
     1741 tcagaccgag aacgacgagc cgcaggaaca ctgggcgctg gcgttggggt tgtcgaactt
     1801 gaagccggta tgggtcaggg tatcgacgaa gtcgatggtg acgttgtcga tcatcggcat
     1861 ggtcatcggg tcgaccagca gcttgacgcc atcgacctcg tgttcccaat cgtcctcggc
     1921 cttctcggcc tccagcttca tgccgtattg caggccggag cagccgccgc cggagatcat
     1981 caggcgcagg ccgataaccg gcgtttcgga accgcggatg aagcgttgta cggccttcgt
     2041 ggcggcgggg gtcagattga tcatggcaat tctcccaggg gtggatgagc tatccattgc
     2101 aagccccgtg ccatcccggg aaacaatctc aaaccattga attcaatgta ttgttgcgcc
     2161 gcaccaggtt tgtcggcttt acaacaatca acccaggacg tgttcgtaag cgcgccgcat
     2221 caggtcgcgc tcgatggcgc tcagcgggcg cgcctcggcg atcgtgatga aacgaccgcc
     2281 cagccgctcg gcagcggcga acggcggatc gatgctggtg aagccgccga gcagcaccgc
     2341 cacgtcaccg gccggcgtat cgacccagca gtggaaatcg ggcaccgcct cgatcgcccc
     2401 ttcggccagc ccgagttgcc cttccgcttc gcgcagcggc gcaccgggat gcggttgcac
     2461 cgtcggcgac cagtcttccg gacgcagggc agcggcgccc ggcaggggtg taaacagcgc
     2521 gacgccgcag ggcagacaga gatagcgcaa gcggccactg gtgccctgct tgtgcaggag
     2581 aagcaaacgc gtgtcgaggt tcatgccgga tactccacga tgaccgcttc gccgcgccag
     2641 gcctggggcc gcccccagac ctgcacggca tcgacgtagg cgccggactg cgtaggatgc
     2701 ggcagcagca cctggtattc ggcgaacacc cggccgtccg ggaagaaggt cgccttgccg
     2761 aaacgctcgc cgccgcgcca gacggcgacg acgctctcct cctgcgagaa ggggtcgcgc
     2821 accgtctcga agccggcggc cgaccagcgc ggctcgtcgc cgatcgacag accgagcttg
     2881 ccgacctgct cgcgcagcgc ctggcacagc gactcgccga tttccgggga gggcagcgtc
     2941 atcgcccctc cccgtggccg gaggcgctca agccgccgcc cgcgccttct gcgccgccag
     3001 cagccgctgg ttggcttcgg cccaggcctg gcaggcgtcg taggtcgatt gggcgatgcc
     3061 cggaatctcc tggtaggccg ccggcaggcg gtcttccacg aggtcgtgca tctgcgaggc
     3121 ccattcggaa gcgatccgct tcagtttctt cacttccttg tcgagggcgg caagttcctc
     3181 gtcgctcacg gcactctcct cacaggccgg caacggccgg gtactggccg atgcgctcga
     3241 gggcgatgcc aagcaacttg tcggcctcat ccttcatctt ggacaggctg gcgaagccga
     3301 agcgatgcac gtcgcgcagg gtcttgtcga cggcgaccag cttgccgacg gtgatcagcg
     3361 cgcggccgaa accttcgtgc gtcaggtgca ccagcggcac cgccagcagc ttgcattcct
     3421 tctcgatcag cacggcgatc gcgttgtaga aggccttgac gcgcgagatc gtctcgtcgt
     3481 ccgggtcgcc gatcagcggg atggtcgcct tgcgttcctt gctcatgatg aaggggtcga
     3541 ggatctgctc gacggtccac ccgctgtagg tgccgtaggt atcgagcgcg cgcatctggc
     3601 gcgccatctc cttgatgaag tcggtctcga aaatcggatc agtgctcatg gtgaaactcc
     3661 ttgggggaaa ttcttgcggg ttggggccgg gccagcagtt gcggtgccgg catcagaccg
     3721 ggcaacaggc cgatgaccag gcgggcgagc acgatggcga tcaggaaacc gatcat
//
LOCUS       DAOSDX010000180         3770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8586_length_3770_cov_3.245760, whole genome shotgun sequence.
ACCESSION   DAOSDX010000180 DAOSDX010000000
VERSION     DAOSDX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8586_length_3770_cov_3.245760"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLN02_05155"
     CDS             <1..599
                     /locus_tag="PLN02_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI51743.1"
                     /translation="KGMNEIRNQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVL
                     ALNAAIQAASAGDAGRGFTVVAEEVQRLAERSGEATKQIAAIVKTIQTDTQDAVSAME
                     QSTQGVVEGAKLSDAAGQALAEIGQVSRNLAELIESISSSTQSQADSATNVARLMQDI
                     LRVNEQTTAGTQRTAQAVDELSALASELKGSVAGFKVD"
     gene            617..>3770
                     /locus_tag="PLN02_05160"
     CDS             617..>3770
                     /locus_tag="PLN02_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOI51744.1"
                     /translation="MNAATEFDLGPLTWVKGEIDQALGRADEALGLFADNGDRTQLKF
                     CRTHVHQVHGALDIVGLNGITELTESLEEVLKALEEERLVAGDAALPVVREAIAALRQ
                     YLDDLMAGEPHQPLRMLPVCSRLAELRGLPPVHPGDLFYPDLSLRPPRSGVVPPVLPP
                     EQMQAVLRKQRMRFQKGLLNWLTRPEQRASGKQAMAEALAAIEALQPTPGARSFWWVS
                     QAFLEALDDPEVSADQLARQLCARIDLQIRRLLEGSGNLAERVMREALYYVARTPATP
                     DSLVAQAQQIYALPAQVPSLAAVQTLPKSQEQGLRRLRELLAGVEEVWNKYCAGSPAS
                     LNTFCDQARACAQVTADLGHTDLKRLGQALAAISGWLQEDAKRFNDGVAMEVATTILL
                     LQNAHDNFRHLGSDFAQQVDLMVDRIHACIAGRPAAGGELPLLDEMTRRAQEKLLIAQ
                     VGREIQSNLAQIEQTLDAFFRAPAKGFSMAQVEGPLKQVSGALAMLGHFPAVSSLKNS
                     IAHIAGFAAADYQPQESDFQRVADELSVLGFFVDALQHGESSFDAFVRRLNGEAAVVV
                     AEEAPTPTVEAEVAAQTRQARALAEALREAPDDRRLQDELKQNLENLQKDADLVADRE
                     LGESAKAALAALESGGPVDALAGLLTPQVEAPQPSAETLLLAESSHETIDAELLAIFI
                     EEGHEVLAEMATQKAALAADPLDREALTTIRRCTHTLKGSGRMVGLADFGETAWAVEQ
                     TLNLWLRQDMAVSPELLAMVDEEQRVFAVWIEHLAQGSGMAPDPSGLVAQAVALRGGT
                     GDALPAVPTPLAPPPLAVDTAPAESPAEPEAPAEPTSEPLLDAFEIDLPEASEVAEEL
                     AEVEAAVVSTAAAPAADSAQTLYEIFRTEAQGHLQSLAESFAVLEAEPATPTNFEMTR
                     AAHTLGGIAAIVGLKPLNHLAIALEHALLRRDAALQPDSLEGLEAMRQAILMLHEMYA
                     GLAEERAPVEQPALVAALEDSYPPAPAAEVDDSGARVIPLPGLSGEFTLDRASEAAPP
                     PPAEPVRQLVDELDEQLLPIF"
BASE COUNT          678 a         1297 c         1248 g          547 t
ORIGIN      
        1 tcaagggcat gaacgaaatc cgcaaccaga tccaggaaac ctcgaagcgg atcaagcgcc
       61 tcggcgaaag ctcgcaggaa atcggcgaaa tcgtcgaact gatttccgac attaccgaac
      121 agaccaacgt gctggcgctg aacgccgcca ttcaggcggc ttccgccggt gacgccgggc
      181 gcggcttcac ggtcgttgcc gaggaagtgc agcggctggc cgaacgctcc ggcgaagcga
      241 ccaagcagat cgcggcgatc gtcaagacga ttcagaccga tacccaggac gcggtgtccg
      301 cgatggagca atcgacccag ggtgtggtcg agggggccaa actgtccgac gccgccggtc
      361 aggcgctggc cgagatcggc caggtgtcgc gcaacctggc cgaactgatc gagagcattt
      421 ccagttcgac gcagagccag gccgattcgg cgaccaatgt ggcccgcttg atgcaggaca
      481 tcctgcgcgt caacgagcag accacggcag gtacccagcg gaccgcccag gcggtggatg
      541 aactgtcggc actggcctcc gaactcaagg gctcggtggc aggcttcaag gtcgactgac
      601 cagcgaacga gagagcatga acgcggcaac ggaattcgat ttgggcccgc tgacgtgggt
      661 caagggcgag atcgaccagg cgctgggacg cgccgacgaa gcgctgggct tgttcgccga
      721 caacggcgac cggacgcagt tgaagttctg ccgcacccat gttcaccagg tccatggcgc
      781 gctcgacatc gtcggtctca atggcatcac cgaactcacc gagtcgctcg aggaggtcct
      841 caaggcgctc gaggaagagc gcctggtggc tggcgacgcc gcgctgccgg tggtccggga
      901 ggcgatcgcc gcgctgcgcc agtacctcga cgacctgatg gcgggcgagc cgcaccagcc
      961 cctgcgcatg ctgccggtat gcagccgact ggccgagttg cgtggcctgc cgccggtgca
     1021 cccgggcgat ctcttctacc ccgacctcag cctgcgcccg ccgcgttccg gcgtggtgcc
     1081 gccggtcctg ccgccggagc agatgcaggc cgtgctgcgc aagcagcgca tgcgcttcca
     1141 gaagggcttg ctcaactggt tgacccgccc cgaacagcgc gccagcggca aacaggcgat
     1201 ggccgaggcg ctggcggcga tcgaggcatt gcagccgacg cccggtgccc gttccttctg
     1261 gtgggtgtcg caggctttcc tcgaagcgct cgacgatccc gaagtttccg ccgatcagct
     1321 ggcgcgtcag ctatgcgccc gcatcgacct gcagatccgc cgcctgctcg aaggctccgg
     1381 caacctcgcc gaacgggtga tgcgcgaggc cttgtactac gttgcccgta ccccggccac
     1441 gccagattcg ctggtcgccc aggcgcagca gatctacgcc ttgccggcgc aagtgcccag
     1501 cctggccgcc gtgcagaccc tgcccaagtc ccaggaacag ggcctgcgtc gtctgcgcga
     1561 gttgctcgcc ggtgtcgagg aagtctggaa caagtactgc gccggtagtc cggccagcct
     1621 gaataccttc tgcgatcagg cacgagcctg cgcccaggtt accgccgacc tcggtcacac
     1681 cgatctcaag cgcctcggcc aggcgctggc ggcaatctcc ggctggctgc aggaagacgc
     1741 caaacgcttc aacgacggcg tggccatgga agtggcgacg accatcctgc tgttgcagaa
     1801 cgcgcacgac aatttccgcc atcttggcag cgacttcgcg cagcaggtcg atctcatggt
     1861 cgaccgcatc catgcctgta tcgccggtcg cccggcggcc ggtggcgaat tgccgctact
     1921 cgacgagatg acgcggcgcg cccaggaaaa gctgctgatc gcccaggtcg ggcgcgagat
     1981 ccagagcaac ctggcgcaga tcgaacagac gctcgacgcg ttcttccggg cgcccgccaa
     2041 gggtttctcg atggcccagg tggaaggccc gctcaagcag gtcagcggcg cgctggcgat
     2101 gctcggtcac ttcccggccg tcagcagcct gaagaacagc attgcccaca tcgcaggttt
     2161 cgccgccgcc gattatcaac cgcaggaaag cgatttccag cgcgtcgccg acgaactttc
     2221 ggtgctcggc ttcttcgttg atgccctgca gcatggcgag agcagtttcg acgccttcgt
     2281 ccgccgcctg aacggcgagg ccgcggtcgt cgtcgccgag gaagcgccga cgccgaccgt
     2341 cgaagccgaa gtcgccgccc agacccggca ggcgcgcgcg ctcgccgaag ccctgcgcga
     2401 ggcgccggat gaccgtcgct tgcaggacga actgaaacag aacctggaaa acctgcagaa
     2461 ggatgccgac ctcgtcgccg accgcgaact cggcgaatcg gcgaaggcgg cgctggcggc
     2521 gctcgaatcc ggtggcccgg tagacgcgct ggccggtctg ctcacgccgc aggtcgaagc
     2581 cccgcagccg tcggcggaaa ccctgctgtt ggccgaatcg agccacgaga cgatcgacgc
     2641 cgaactgctc gccatcttca tcgaggaagg tcacgaggtg ctggccgaga tggcgacgca
     2701 gaaggcggca ctggccgccg atccgctcga tcgcgaggcg ctgacgacga tccgccggtg
     2761 cacgcatacc ctcaagggct ccgggcgcat ggtcgggctt gccgatttcg gcgaaaccgc
     2821 ctgggccgtc gagcagacgc tgaacctgtg gttgcgccag gacatggcgg tgagcccgga
     2881 actgctggcg atggtcgacg aggaacagcg cgtctttgcc gtctggatcg agcatctggc
     2941 gcaaggcagc ggcatggccc ccgatccctc gggcttggtg gcgcaggcgg tcgcgctacg
     3001 cggcggcacg ggcgatgccc tgccggcggt gccaacgccg cttgccccgc cgccgcttgc
     3061 cgtcgacacg gcgcccgccg agtcgcccgc agaaccggaa gcgccggcag aaccgacaag
     3121 cgaacccctg ctcgatgcct tcgagatcga cctgccggaa gccagcgagg tcgccgagga
     3181 actggccgag gtcgaagccg cggtcgtcag caccgccgcc gcaccggccg ccgacagcgc
     3241 gcagacgctg tacgaaattt tccggaccga agcccaaggt catctgcagt cgctggcgga
     3301 gtccttcgcc gtgctcgaag ccgagccggc gacgccgacc aatttcgaaa tgacgcgggc
     3361 ggcccacacg ctcggcggca tcgccgccat cgtcggcctg aaaccgctca accacctcgc
     3421 catcgccctc gaacacgccc tgctgcggcg cgatgcggcg ctccagccgg acagtctgga
     3481 aggcctggaa gcaatgcgcc aggcgatcct gatgttgcat gaaatgtacg ccgggctggc
     3541 cgaagaacgg gcgccggtgg aacagccggc gctggtggcc gcgctggagg acagttaccc
     3601 gccggccccg gccgcggaag tcgacgacag tggcgctcgc gtcatcccgc tgcccggcct
     3661 gagcggtgaa ttcaccctcg accgcgccag cgaagccgca ccgccaccgc cagcggaacc
     3721 ggtccgccag ctggtcgacg aactcgacga acaattgctg ccgatcttcc
//
LOCUS       DAOSDX010000181         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8600_length_3765_cov_3.402426, whole genome shotgun sequence.
ACCESSION   DAOSDX010000181 DAOSDX010000000
VERSION     DAOSDX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_3765_cov_3.402426"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLN02_05165"
     CDS             complement(<1..808)
                     /locus_tag="PLN02_05165"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOI51745.1"
                     /translation="MPGATLFAFLRRVAVAFYRNQGFLMAGALAYYALLSLLPLLILS
                     IIGLSQLVEPALLLDAIGRNLDWLLPNQAGLLLADVSAFLDHRGSIGVTMFGTLLFFS
                     ASAFSVLEKALAVIFTHRGERRPRHFLVSAVLPYLLVLMLVLTVLPLTLGVSALQALG
                     DRELLLFGDSRSLASVADILLPLPGIVLVGCVLGLLYLIVPVGRTRLAHAFIGGFAGA
                     LLWEIVRHGLAWYFASASRIGVVYGSLASTVVLLFSLEVAAYPEWHPQARS"
     gene            complement(816..1991)
                     /locus_tag="PLN02_05170"
     CDS             complement(816..1991)
                     /locus_tag="PLN02_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 family protein"
                     /protein_id="HOI51746.1"
                     /translation="MHRWLPFALLAALGGCSLFDSEKEGPALPPDMAHQAKGKTASAW
                     PETLDRDERLRRIVRLIPARARDRQGWAEDLHTAYEHLGVPHATQTYCAAIAIIDQES
                     SFQADPPVPGLPRIVRRELAEREERYRIPKLVVDTALRKTSPDGRTYEARIAALKTET
                     QLNALFEDMIGELPFGRKLLGGFNPVRTGGPMQVSIEFAEQHVRQRKYPYAYDGKLRN
                     EVFTRRGGVYFGAAILLDYAVPYDRIVYRFADFNAGRYSSRNAAFQAALGGLTGRKLG
                     LDGDLLRYDDGKPSATPSEVETLTLELADRLDMSRPQIRRDLLLEKNEAFSRSPLFLK
                     VFELAEADAGKTLPRQILPEIQLKSPKITRKLTTGWFAKRVEDRYLGCLGRGRRFQR"
     gene            complement(2000..3763)
                     /gene="dsbD"
                     /locus_tag="PLN02_05175"
     CDS             complement(2000..3763)
                     /gene="dsbD"
                     /locus_tag="PLN02_05175"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HOI51747.1"
                     /translation="MRLLFLLFAFLASCVHAQEFLDPAVAFKATARALDGRTVEVSYE
                     IAPDYYLYKDKFRVRATDETIVLGPLQLPPGKEKQDENFGRVEVYYKTVHFRVAVERN
                     SSGALPLTLDLVSQGCADAGVCYPPQTQTVLLNLPDPDSTPAAPALGETPSTAAGDES
                     GMIAETLKNAGFWTSLALFFVAGLGLSLTPCVFPMIPILSGIIAGQGHRASKGRGLAL
                     SLAYVLGMAVTYAAAGIAAGLTGTLLSAALQNVWVLGGFALVFVILSFSMFGFYELQL
                     PTSLQSKLSEETGHLQGGRGIGVFLMGALSALIVGPCVAAPLAGALLYIGQTGDALFG
                     GVALFVMALGMGAPLIAVGVAGGSLLPKAGPWMEGVRRAFGVLLLATALWLLSPVIPA
                     LASMLGWAVLLIVPAIYLHALDPLPPHARGWQRFWKGIGILMLLTGAALLVGALAGGR
                     DPLQPLAGLRGSAIAAETPTLPFQKVASLAELDARLASAGQPVMLDFYADWCVSCKEM
                     EKFTFSDPAVRQKLAGFLLLKADVTANSPDDKALLARFGLFGPPGIIFFDPAGQEIKG
                     VRVVGFQDAERFLSSLNRVSG"
BASE COUNT          648 a         1167 c         1412 g          538 t
ORIGIN      
        1 ggctgcgtgc ttgcgggtgc cattccgggt aggcggcgac ttcgaggctg aagagaagga
       61 cgacggtgct ggcgagcgaa ccgtaaacga cgccgatgcg tgatgccgag gcgaagtacc
      121 aggccagccc gtggcggacg atttcccaca gcagggcacc ggcgaagccg ccgatgaagg
      181 cgtgcgccag gcgggtgcgg ccgaccggca cgatcaggta gagcaggccg agcacgcagc
      241 cgacgaggac gatgccgggc agcggcaaca ggatgtcggc gacgctggcg agcgagcggc
      301 tgtcgccgaa cagcaaaagc tcgcgatccc ccagcgcctg cagcgcgctg acgccgaggg
      361 tgagcggcag cacggtcagc accagcatca ggaccagcag gtagggcagc accgccgaga
      421 ccaggaaatg gcgcggccgg cgctcgccgc gatgggtgaa gatcaccgcc agcgccttct
      481 ccagcaccga gaaggcggaa gcgctgaaga acaggagcgt gccgaacatg gtcacgccga
      541 tcgagccgcg atggtcgagg aaggcgctga cgtcggcgag cagcagtccg gcctggttgg
      601 gcagcagcca gtcgaggttg cggccgatgg cgtcgagcag gagggcgggt tcgaccagtt
      661 gcgagaggcc gatgatgctg aggatcagta gcggcagcag ggagagcagc gcgtagtaag
      721 cgagcgcacc ggccatcagg aagccctggt tgcggtagaa ggcgacggcg acccggcgca
      781 ggaaggcgaa cagggtggcg ccgggcatcg ccgggtcagc gctggaaacg ccggccgcgg
      841 ccgaggcagc cgaggtagcg gtcttcgacc cgtttggcga accagccggt ggtcagcttg
      901 cgggtgatct tcggactctt cagctggatt tccggcagta tctggcgcgg cagcgtcttg
      961 ccggcatcgg cttcggccag ttcgaagacc ttgaggaaaa gcgggctgcg cgagaaggct
     1021 tcgttctttt ccagcagcag gtcgcggcgg atttgcggcc ggctcatgtc gagccggtcg
     1081 gcgagttcga gggtcagcgt ctccacctcg ctcggcgtgg cggagggctt gccgtcatcg
     1141 tagcgcagca ggtcgccgtc gagcccgagt ttgcggccgg tcagcccgcc cagcgccgcc
     1201 tggaaggcgg cgttgcggct gctgtagcgg ccggcgttga agtcggcgaa gcgatagacg
     1261 atgcgatcgt agggcaccgc gtaatcgagc aggatggcgg cgccgaaata gacgccaccg
     1321 cgccgggtga acacctcgtt gcgcagcttg ccgtcgtagg catacgggta cttgcgctgg
     1381 cgcacgtgct gttcggcgaa ctcgatcgac acctgcatcg gtccgccggt gcgtaccggg
     1441 ttgaagccgc cgagcagttt ccggccgaac ggcagttcgc cgatcatgtc ctcgaacagc
     1501 gcgttgagct gggtctcggt cttcagggcg gcgatccgcg cttcgtaggt ccggccgtcg
     1561 ggcgaggtct tgcgcaaggc cgtatcgacc accagcttcg ggatgcggta gcgctcctcg
     1621 cgttcggcca gctcgcggcg gacgatgcgg ggcaggccgg ggaccggcgg atcggcctgg
     1681 aaactcgact cctggtcgat gatggcgatc gccgcgcaat aggtctgggt ggcgtgcggc
     1741 acgccgaggt gctcgtaggc ggtgtgcagg tcttccgccc agccctggcg atccctggcg
     1801 cgggcgggaa tcaggcggac gatgcggcgt agccgctcgt cgcggtccag ggtttccggc
     1861 caggccgagg cggtcttgcc cttggcctgg tgcgccatgt ccggcggcag cgccggtcct
     1921 tccttctcgc tgtcgaacag cgagcagccg ccgagcgcgg caagcagggc gaacggcagc
     1981 cagcgatgca cgatgcgctt tagccgctta cgcggttcag gctggagagg aagcgttcgg
     2041 cgtcctggaa gccgacgacc cttacgccct tgatttcctg acccgccgga tcgaagaaga
     2101 tgatcccggg cggtccgaac aggccgaagc gggcgagcag cgccttgtcg tcggggctgt
     2161 tggcggtgac gtcggccttc agcagcagga aaccggcgag cttctggcgc accgccgggt
     2221 cgctgaaggt gaatttctcc atttccttgc acgagacaca ccagtcggcg tagaaatcga
     2281 gcatcaccgg ttgcccggcg ctcgccaggc gggcgtcgag ttcggccagc gaagcgactt
     2341 tctggaacgg cagggtcggc gtttcggcgg cgatcgcgct gccgcgcagg ccggccagcg
     2401 gttgcagcgg gtcgcggccg ccggccaggg cgccgaccag cagtgcggcg ccggtgagca
     2461 gcatgaggat gccgatccct ttccagaagc gctgccagcc gcgggcgtgc ggcggcagcg
     2521 gatcgagcgc gtgcaggtag atggcgggga cgatcagcag caccgcccag ccaagcatgc
     2581 tggccagcgc ggggatcacc ggcgacagca gccacagcgc ggtggccagc agcagcacgc
     2641 cgaaggcccg acggacgcct tccatccacg gcccggcctt gggcagcagc gaaccgccgg
     2701 cgacaccgac ggcgatcagc ggcgcgccca tgccgagtgc catgacgaag agggcgacgc
     2761 cgccgaacag cgcgtcgccg gtctggccga tgtagagcag cgcgccggcg agcggcgcgg
     2821 cgacgcaggg accgacgatc agcgccgaca gcgcgcccat caggaagacg ccgatgccgc
     2881 gtccgccctg caggtggccg gtttcttccg aaagtttgct ctgcagcgag gtcggtagct
     2941 gcagttcgta gaagccgaac atcgagaacg agaggatcac gaagaccagc gcgaagccgc
     3001 ccagcaccca gacgttctgc aaggccgccg agagtagcgt gccggtgagg ccggcggcga
     3061 tgccggcggc ggcgtaggtg accgccatgc ccagcacgta ggcgagcgac agcgccagtc
     3121 ccctgccctt cgacgcccgg tggccctggc cggcgatgat gccggagagg atggggatca
     3181 tcgggaagac gcagggggtc agcgagagac cgaggccggc gacgaagaag agcgccaggc
     3241 tggtccagaa accggcgttc ttcagcgtct cggcgatcat tccgctttcg tcaccggcgg
     3301 cggtcgacgg cgtttcgccc agcgccggcg cggccggcgt gctgtccggg tcgggcaggt
     3361 tgagcagtac tgtctgcgtc tgcggcggat agcagacgcc ggcatcggcg cagccctgcg
     3421 agacgaggtc gagggtcagc ggcaaggccc cggaactgtt gcgctcgacg gcgacgcgga
     3481 agtgcaccgt cttgtaatag acctcgaccc ggccgaagtt ctcgtcctgc ttttccttgc
     3541 ccggtggcag ttgcagcggg ccgaggacga tcgtttcatc ggtggcgcgg acgcggaact
     3601 tgtccttgta caggtagtag tcgggggcaa tttcgtaact gacctcgacc gtccggccgt
     3661 cgagcgcccg cgccgtggcc ttgaaggcga cggccggatc gaggaattcc tgggcatgga
     3721 cgcaggaagc caggaaggcg aagagaagga acaggaggcg catgg
//
LOCUS       DAOSDX010000182         3758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8624_length_3758_cov_3.525790, whole genome shotgun sequence.
ACCESSION   DAOSDX010000182 DAOSDX010000000
VERSION     DAOSDX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8624_length_3758_cov_3.525790"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLN02_05180"
     CDS             <1..487
                     /locus_tag="PLN02_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI51748.1"
                     /translation="IAVQASLTGHLVLATLHTNDAVSAATRLTDMGIEPFLLSSSLLG
                     VLAQRLVRRICPHCRQPGPAASDAGLPAEIGRLWQAVGCPHCAHTGYLGRLGIYELLE
                     IDDALRPLVHQQSGEAALRAAARDPARGASLLRLLHEDGLRWLEAGETSLEELLRVTR
                     A"
     gene            500..1699
                     /gene="gspF"
                     /locus_tag="PLN02_05185"
     CDS             500..1699
                     /gene="gspF"
                     /locus_tag="PLN02_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOI51749.1"
                     /translation="MPAFHYRAIALDGRESSGTVDAESSRQARASLREQGLLAVDLQA
                     ARSAGTQRKLPRLGNTALMLLTRQWSTLLAAGIPVERALAALAEQSDDAPTRSLLGAI
                     RDELQAGHPLHRALENFPQTFNRLYCALVAAGEQSGQLDQVMQRLADSLESSGALRQK
                     VLQATIYPVLVVIVASAVIGALMLYVVPQVVAVFNSGKHTLPFLTRALIAVSDLLRTT
                     WPLLAVVFGGGLWGFRRAWRNEDFRHARQLQLLRLPVLGRLLQALDSARLAQTMAILV
                     ASGVPLLAALDAGCAVLWLLPLRRALATASGEVREGASLHRALARSGRFPPLLVHMIG
                     SGEASGRLGEMLEKAARQQSDEVAARLGTATALLEPLLILAMGGIVLVIVLAILQPII
                     EINQLLH"
     gene            1705..2139
                     /gene="gspG"
                     /locus_tag="PLN02_05190"
     CDS             1705..2139
                     /gene="gspG"
                     /locus_tag="PLN02_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOI51750.1"
                     /translation="MPKTPARQTGFTLIEVMVVVVILGILAALVVPKVMSRPDEARQV
                     AARQDIGGLLQALKLYRLDNRRYPSSEQGLRALVEKPAQPPLPDNWKPGGYVERLPLD
                     PWGQPYQYLNPGLRGEIDVFSFGADGVAGGEGADADIGNWAL"
     gene            2111..2584
                     /locus_tag="PLN02_05195"
     CDS             2111..2584
                     /locus_tag="PLN02_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI51751.1"
                     /translation="MRTSATGRSEAGQAGFTLVEILVALAIVAILAGGVALTLPDPAR
                     AAQQATAQALHAQAASAARRARSEAQPWAWQVDADGGRLLVAADGRWQAAGKPVSSSG
                     LAVGGPEIDGVRRPPGSAIVFAAVPPIFAVDLQGDGRRWRIAGQPNGRVTLEQLP"
     gene            2581..2952
                     /gene="gspI"
                     /locus_tag="PLN02_05200"
     CDS             2581..2952
                     /gene="gspI"
                     /locus_tag="PLN02_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOI51752.1"
                     /translation="MNARADGFTLLEILVALAILALALGAAARAGGMATETTGGLRER
                     LLAGWVAENRLALLHAERAWPDPGSSLGSSRLAGRDFAWRMQVTAAAQGQFRRVEIVV
                     RAVEAAPDSVAARFTGYLKQP"
     gene            2949..3509
                     /locus_tag="PLN02_05205"
     CDS             2949..3509
                     /locus_tag="PLN02_05205"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOI51753.1"
                     /translation="MRQRGFTLIEILVAVSLLAVLGVLGYRGVAAASANAAHLSGEAG
                     RWQDIALALARVGDDSRQAIAVAGRAADGRELPAWQLGGQPAQLTLTRAVAGGELRRL
                     AYRCQAGGLELLLWPAYDAPAPRVSHRLLAGITGCEMSVLDHESRWLAVWPQRDGRTL
                     PRALRLRLSLAEGGMIERIFDVAAAE"
     gene            3490..>3758
                     /locus_tag="PLN02_05210"
     CDS             3490..>3758
                     /locus_tag="PLN02_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOI51754.1"
                     /translation="MSPRPNERQRGAAVVAALLVVALVSATMATLLTDLDAWIDDVDS
                     ARDKAQATEQARNAVAYARALLAADGASNAIDTLDEDWARELPPLR"
BASE COUNT          508 a         1465 c         1284 g          501 t
ORIGIN      
        1 gattgccgtc caggccagcc tgaccggcca cctggtgctg gccacgctgc acacaaacga
       61 cgcggtgagc gccgccaccc ggctgaccga catgggcatc gaacccttcc tgctctcgtc
      121 gtcgctgctc ggcgtcctcg cccagcgcct ggtgcgccgc atctgcccgc actgccggca
      181 gcccggcccg gcggccagcg acgccggcct gccggccgag atcggccgcc tctggcaggc
      241 cgtcggctgc ccgcactgcg cccacaccgg ctatctcggg cggctgggca tctacgaact
      301 gctcgagatc gacgacgcgc tacgcccgct ggtccatcaa caaagtggcg aggccgcgct
      361 gcgcgcggcg gcgcgcgacc ccgcccgcgg ggcgagcctg ctgcgcctgc tgcatgagga
      421 cgggctgcgc tggctggagg ccggcgagac ctcgctcgaa gaactgctgc gcgtgacgcg
      481 cgcctgacgg acgccgagca tgcccgcctt ccactaccgc gccattgccc tcgacggccg
      541 tgaaagcagc ggcaccgtcg atgccgagag cagccgccag gcacgcgcca gcttgcgcga
      601 gcagggcttg ctcgccgtcg acctgcaggc ggcgcggagc gccggtaccc agcgcaagct
      661 gccgcgcctc ggcaacacgg cgctgatgct gctcacccgc cagtggtcga cgctgctcgc
      721 tgccggcatc ccggtcgagc gggcgctggc ggcgctcgcc gaacagagcg acgacgcccc
      781 gacgcgcagc ctgctcggcg ccatccgcga cgaactgcag gccggccatc cgctgcaccg
      841 ggcgctggaa aactttccgc agaccttcaa ccgcctctac tgcgcgctgg tcgccgccgg
      901 cgagcagagc ggccagctcg accaggtcat gcagcgcctc gccgacagcc tggaaagcag
      961 cggcgcgctg cgccagaagg tgttgcaggc gaccatctac ccggtgctcg tggtcatcgt
     1021 cgccagcgcc gtgatcggcg ccctgatgct ctacgtcgtg ccgcaggtgg tcgccgtctt
     1081 caacagcggc aagcacacgc tgcctttcct gacccgggcg ctgatcgccg tcagcgatct
     1141 cttgcgcacc acctggccgc tgctcgccgt cgttttcggc ggcggcctgt ggggcttccg
     1201 ccgcgcctgg cgcaacgagg atttccgcca cgcccgccag ttgcaactgc tgcgcctgcc
     1261 ggtcctcggc cgcctgctgc aggcgctcga cagcgcccgc ctggcgcaga ccatggccat
     1321 cctggtggcc agcggcgtgc cgctgctcgc cgccctcgac gccggttgcg ccgtgctctg
     1381 gctgctgccg ctgcgccgcg cgctggcgac ggcgagcggc gaagtgcgcg aaggcgccag
     1441 cctgcaccgc gccctcgccc gctccgggcg ttttccgccg ctgctggtgc acatgatcgg
     1501 cagcggcgaa gcgtccgggc gcctcggcga aatgcttgaa aaggccgccc gccagcaaag
     1561 cgacgaggtc gccgcccgcc tcggcaccgc caccgcgctg ctcgaaccgc tgctcatcct
     1621 ggccatgggc ggcatcgtcc tggtcatcgt cctcgccatc ctgcaaccga tcatcgagat
     1681 caaccaactg ctgcattgaa cgccatgccc aaaacccccg cccgccagac cggcttcacc
     1741 ctgatcgaag tcatggtcgt cgtcgtcatc ctcggcatcc tcgccgccct cgtcgtgccc
     1801 aaggtgatga gccgacccga cgaggcgcgc caggtcgccg cccgccagga catcggcggc
     1861 ctgctgcagg cgctcaagct ctaccgcctg gacaaccgcc gctacccgag cagcgaacag
     1921 ggcctgcgcg ccctggtcga gaaaccggcg cagccgccgc tgcccgacaa ctggaagccc
     1981 ggcggctacg tcgagcgcct gccgctcgat ccctggggcc agccctacca gtacctgaac
     2041 cccgggctgc gcggcgagat cgacgtcttc agcttcggcg ccgacggtgt cgcgggtggc
     2101 gagggcgcag atgcggacat cggcaactgg gcgctctgag gccgggcagg ccggcttcac
     2161 gctggtcgag atcctggttg cgctcgccat cgtcgccatc ctggccggcg gcgtcgcgct
     2221 gaccctgccc gatccggcgc gcgccgccca acaggcgaca gcccaggccc tgcacgcgca
     2281 ggcggcgagc gccgcccgtc gcgcgcgcag cgaggcgcaa ccgtgggcct ggcaggtcga
     2341 tgccgacggc ggacgcctgc tggtggccgc cgacggacgc tggcaagcgg ccggcaagcc
     2401 ggtcagcagc agcggcctgg ccgtcggcgg accggagatc gacggcgtcc ggcggccacc
     2461 cggcagcgcc atcgtctttg ccgccgtgcc gccgatcttc gccgtcgacc tgcagggcga
     2521 cggccgccgc tggcgtatcg ccggccagcc gaacggccgg gtgacgctgg agcaactacc
     2581 atgaacgccc gggccgacgg tttcaccttg ctggaaatcc tcgtcgccct cgccatcctc
     2641 gctctcgccc tgggcgccgc ggcgcgcgcc ggcgggatgg caacggagac gaccggcggc
     2701 ctgcgtgaac gactgctcgc cggttgggtg gcggagaacc ggctggccct gctgcacgcc
     2761 gagcgcgcct ggcccgaccc gggcagcagc ctgggcagca gccggctggc cgggcgcgat
     2821 ttcgcctggc gcatgcaggt gacggccgcc gcccagggtc agttccgccg cgtcgagatc
     2881 gtcgtccgcg ctgtcgaagc ggcgcccgac agcgtcgccg cgcgcttcac cggctacctg
     2941 aagcaaccgt gaggcagcgc ggcttcacgc tgatcgagat cctcgtcgcc gtcagcctgc
     3001 tggcggtgct cggcgtcctc ggttaccgcg gcgtcgccgc ggccagcgcc aacgccgccc
     3061 acctgagcgg cgaagccggg cgctggcagg acatcgcgct agccctggcc cgggtcggcg
     3121 acgacagccg gcaggcgatc gccgtcgccg gccgcgccgc cgatgggcgc gaacttcccg
     3181 cctggcaact gggcggccaa ccggcccagt tgacgctgac ccgcgccgtc gctggcggcg
     3241 aactgcgccg cctggcctac cgctgccagg ccggcggcct cgaactgctg ctctggcccg
     3301 cctacgacgc gccggcgccg cgagtcagcc accgcctgct cgccggaatc actggctgtg
     3361 aaatgagcgt gctcgaccac gaaagtcgct ggctggccgt ctggccacag cgcgacggcc
     3421 ggacgctgcc gcgcgccctg cgcctgcgcc tgagtctggc cgagggtggc atgatcgaaa
     3481 ggatcttcga tgtcgccgcg gccgaatgag cgccagcgcg gcgccgccgt cgtcgccgcg
     3541 ctgctcgtcg tggccctggt cagcgcgacc atggcgacgc tgctgaccga cctcgacgcc
     3601 tggatcgacg acgtggacag cgcccgcgac aaggcccagg ccaccgagca ggcgcgcaac
     3661 gccgtcgcct acgcccgcgc cctgctcgcc gccgacggcg ccagcaatgc catcgacacg
     3721 ctggacgagg actgggcgcg cgaactgccg ccgctgcg
//
LOCUS       DAOSDX010000183         3736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8687_length_3736_cov_2.861722, whole genome shotgun sequence.
ACCESSION   DAOSDX010000183 DAOSDX010000000
VERSION     DAOSDX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8687_length_3736_cov_2.861722"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLN02_05215"
     CDS             <1..469
                     /locus_tag="PLN02_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOI51755.1"
                     /translation="PIAGLGHRIDTSGLKASALREWVRQFIESQPGQGLTLMFESFGF
                     KHGLPLDADLVFDVRCLPNPHYDPALRPLTGRDRPVAEFLECDPDVVRMREDIGRFVS
                     DWLPSYIRDNRNYLTVAIGCTGGQHRSVYLAETLARRFAGQARVLVRHRTLAG"
     gene            469..843
                     /locus_tag="PLN02_05220"
     CDS             469..843
                     /locus_tag="PLN02_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOI51756.1"
                     /translation="MRWLALIRPGDWLVFLLVAALVGFSIPQFWQGGLADRAIVRQEG
                     KVFAEVDLRTRREILVPGPLGVTRIAVEPGRARVVADPGPRQYCVKQGWLMRPGEIAI
                     CAPNRVSLQITGRTKVYDSLSY"
     gene            824..1357
                     /locus_tag="PLN02_05225"
     CDS             824..1357
                     /locus_tag="PLN02_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOI51757.1"
                     /translation="MTRSVIELAVTPEDRRVAWLATAAVGLSLVDAAIPTPLPGIKPG
                     LANIVTLIVLLRYGWGTAVWVSVLRVLAGSLLLGYFLAPGFFLSAAGTTLSLLTLGLA
                     RHLPARWFGPVSLSILAALAHIGGQLLLARAWLIPHDGVFLLLPVFAAAALFFGTING
                     LIAAKLLADSPAPESTP"
     gene            1354..1965
                     /locus_tag="PLN02_05230"
     CDS             1354..1965
                     /locus_tag="PLN02_05230"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HOI51758.1"
                     /translation="MSATVCLALTGASGLPYGLRLLECLLGAGCRVQLLYSQAAQIVA
                     RQELDFDLPSRPADAKAALLARLPAVDGEKLQVFGREEWFAPVASGSNPPDAMVVCPC
                     SMGTLAAIAQGLSDKLIERAADVVLKEGRKLVLVPRETPFSAIHLENMLRLSRAGAVI
                     LPPSPGFYHHPQSVQDIVDFVVARVLDQIAVPHTLMQRWGEDR"
     gene            1965..2594
                     /locus_tag="PLN02_05235"
     CDS             1965..2594
                     /locus_tag="PLN02_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOI51759.1"
                     /translation="MGWFDFIRAPGGGGVETLEIPLFPLNTVLFPGGVQPLKVFEQRY
                     LDMAAACLKDNTPFGICLIDAGAEVGEAAVPHTVGTLASIANWEMEQLGILMLDAIGG
                     RRFRILDHEVGADRLLRATVELLAEPEVPLPQNRERLLPLLQRVVGDLGPARMPEPHR
                     YDDAGWVGYRLTEVLPIQNLAKQKLLELDDPLARLEILETFLDQRKLLG"
     gene            complement(2642..2950)
                     /locus_tag="PLN02_05240"
     CDS             complement(2642..2950)
                     /locus_tag="PLN02_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOI51760.1"
                     /translation="MNRPLQTRKLLTIVCEAALEKRLEKELPGFGAHGYTISDARGNG
                     AHGRRDAAWPASANIRVEVLCDEAVAEAVVRHLEAQYFSSYQLVVFLSDVQVLRAEKF
                     "
     gene            complement(2947..>3736)
                     /locus_tag="PLN02_05245"
     CDS             complement(2947..>3736)
                     /locus_tag="PLN02_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HOI51761.1"
                     /translation="PVGELLFAAGVVVAAGALIPLIAFPVLARLGKLPRADAASIAAH
                     YGSVSVVTFAVASTFLLGRGESAEGYMTVFLVLLEFPALMIGIWLARRGEGSADWGKV
                     LHEVFAGKSIVLLLGGLAIGWVAGSEGLKPLDKLFFDLFKGMLAIFLLEMGLVAASRI
                     GDLRRAGWFLAGFAVAMPLVGAGLGIVTGLLLGLSVGGTTLLATLYASASYIAAPAAM
                     RIAVPEANPGLSIGAALGITFPFNLLLGIPAYHWAAVTLHGVFA"
BASE COUNT          596 a         1370 c         1174 g          596 t
ORIGIN      
        1 gccgatcgcc ggtctcggcc accgcatcga caccagcggc ctgaaggcca gcgccctgcg
       61 ggaatgggtg cgccagttca tagaaagcca gcccggccag ggcctgacgc tgatgttcga
      121 gtccttcggc ttcaagcacg gcctgccgct cgacgccgac ctcgtcttcg acgtccgttg
      181 cctgcccaat ccgcattacg acccggcctt gcgcccgctc accgggcgcg accgaccggt
      241 cgccgaattc ctcgaatgcg accccgacgt cgtccgcatg cgcgaagaca tcggccgttt
      301 cgtctccgac tggttgccca gctacatccg cgacaaccgc aattacctga cggtagccat
      361 cggctgcacc ggtggccagc accgctccgt ctatctggcg gaaacgctgg cccgccgctt
      421 cgccggccag gcccgcgtgc tggtccggca tcgcacgctg gccggttgat gcgctggctg
      481 gcgctgatcc gccccggcga ctggctggtt ttcctgcttg tcgcggcgct ggtcggtttc
      541 agcattccgc aattctggca aggcggcctc gccgaccgcg ccatcgtccg ccaggaagga
      601 aaagtattcg ccgaagtcga tctgcggaca cggcgcgaaa tcctcgtccc cggcccgctc
      661 ggcgtcaccc ggattgccgt cgagccgggc cgcgcccggg tcgtcgccga tcccggaccg
      721 cgccagtatt gcgtcaagca gggctggctg atgcgtcccg gcgaaatcgc catctgcgcc
      781 cccaaccgcg tcagcctgca gatcaccggc cggaccaagg tctatgactc gctcagttat
      841 tgaactcgcg gtcaccccgg aggaccgccg ggtcgcctgg ctggccaccg cagcggtcgg
      901 cctgtcgctc gttgacgcgg cgatcccgac accgttgccg ggaatcaagc cggggctcgc
      961 caacatcgtc acgctgatcg tcctgctccg ctacggctgg ggtaccgcgg tctgggtcag
     1021 cgtcctgcgc gtcctcgccg gcagcctgct gctcggctac ttcctcgccc ccggcttctt
     1081 cctgtcggcc gccggcacga cgctcagcct gctcacgctt ggcctcgccc gccatctgcc
     1141 agcgcgctgg ttcggaccgg tcagcctgtc catcctcgcc gcgctggcgc acatcggcgg
     1201 ccagttgctg ctcgcccgcg cctggctgat cccgcacgac ggcgtcttcc tgctgctgcc
     1261 ggttttcgcc gccgccgcat tgttcttcgg caccatcaac ggcctgatcg cggctaaact
     1321 gctggctgac tctcccgccc cggaatccac tccatgagtg ccaccgtctg cctcgccctg
     1381 accggcgcct ccggcctgcc ctacggcctg cgcctgctcg aatgcctgct cggcgccggc
     1441 tgtcgcgtcc agttgctgta ttcgcaggcg gcgcagatcg tcgcccgtca ggaactggac
     1501 ttcgacctgc cgtcgcgccc ggccgacgcc aaggccgcgc tgctcgcccg cttgccggcg
     1561 gtcgatggcg agaagctgca ggttttcggc cgcgaggaat ggttcgcccc ggtcgcctcc
     1621 ggctccaacc cgcccgacgc gatggtcgtc tgcccctgtt cgatgggcac cctggcggcg
     1681 atcgcccagg ggctttccga caaactgatc gagcgtgccg ccgacgtggt gctcaaggaa
     1741 ggccgcaagc tggtgctggt tccccgtgaa acgccgttct cggcgatcca cctggaaaac
     1801 atgctgcgcc tctcgcgcgc cggtgccgtc atcctgccgc ccagcccggg cttctatcac
     1861 catccgcaga gcgtgcagga catcgtcgat ttcgtcgtcg cccgagtgct cgatcagatc
     1921 gccgttccac acacgctgat gcagcgctgg ggcgaggacc gctgatgggc tggttcgact
     1981 tcatccgcgc ccccggcggc ggcggcgtcg agacgctgga aattccgctg tttccgctca
     2041 acaccgtgct ctttcccggc ggcgtgcagc ccttgaaggt cttcgagcaa cgctacctgg
     2101 acatggcggc cgcctgcctc aaggacaaca cacccttcgg catctgcctg atcgacgccg
     2161 gcgccgaagt cggcgaagcc gcggtaccgc acaccgtcgg cacgctggcc agcatcgcca
     2221 actgggaaat ggagcagctc ggcatcctga tgctcgacgc catcggcggg cgccgcttcc
     2281 gcatcctcga ccacgaggtc ggcgccgacc ggctgttgcg cgccaccgtc gaactgctcg
     2341 ccgaaccgga agtgccgctg ccgcagaacc gcgaacgcct gctgccactg ctccagcgcg
     2401 tggtcggcga cctcggcccg gcgcgcatgc cggaaccgca ccgctacgac gatgccggct
     2461 gggtcggcta ccggctgacc gaagtgctgc cgatccagaa cctggccaag cagaagctgc
     2521 tcgaactcga cgacccgctg gcccgcctgg aaatcctcga gaccttcctc gaccagcgca
     2581 agctgctcgg ctagggcgta tttgcagtaa ccagccgccc gctgcttact atgaacacgc
     2641 cctagaactt ctccgcccgc agcacctgga cgtcggacag gaagacgacc aactggtagc
     2701 tgctgaaata ctgcgcttcc agatgacgga cgacggcttc ggcgaccgcc tcatcgcaca
     2761 gcacctcgac ccggatgttg gcgctcgccg gccaggcggc atcgcggcga ccgtgagcgc
     2821 cgttgccgcg agcatcggaa atcgtgtagc cgtgcgcgcc gaaacccggc aattcctttt
     2881 ccaggcgttt ttccagcgcc gcctcgcaga cgatggtcag cagcttgcgg gtttgcagcg
     2941 ggcgattcat gcgaagactc catgcagggt gacggccgcc caatgatagg ccggaatgcc
     3001 gagcagcaga ttgaacggga aggtaatgcc gagcgcagcg ccgatcgaca ggccgggatt
     3061 ggcttccggc acggcgatgc gcatcgccgc cggcgccgca atgtaggaag cgctggcgta
     3121 gagcgtcgcg agcagcgtcg tgccgccgac cgacaggccg agcaagaggc cggtgacgat
     3181 gccgagcccg gcacccacca gcggcatggc gacggcgaat cctgcaagga accagccggc
     3241 ccggcgcagg tcgccgatgc ggctggcggc gaccaggccc atctcgagca ggaagatcgc
     3301 cagcatcccc ttgaacaggt cgaagaacag cttgtcgagc ggcttcagtc cctcgctgcc
     3361 ggcgacccag ccgatcgcca gaccgccgag caggaggacg atgctcttgc cggcgaagac
     3421 ctcgtgcagc accttgcccc agtcagccga accctcgcca cgccgcgcca gccagatgcc
     3481 gatcatcagc gccgggaatt cgagcaggac gaggaacacg gtcatgtagc cctcggcgct
     3541 ctcgccgcgg ccgagcagga aggtcgaagc aacggcaaag gtgacgacgc tgaccgaacc
     3601 gtagtgggcg gcgatcgacg ccgcgtcggc gcgcggcagt ttgcccaggc gggccagcac
     3661 cggaaaggcg atcagcggaa tcaacgcgcc ggcagcgaca acgacaccgg cggcaaacag
     3721 caactcgccg accggg
//
LOCUS       DAOSDX010000184         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8743_length_3719_cov_3.255731, whole genome shotgun sequence.
ACCESSION   DAOSDX010000184 DAOSDX010000000
VERSION     DAOSDX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8743_length_3719_cov_3.255731"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLN02_05250"
     CDS             complement(<1..817)
                     /locus_tag="PLN02_05250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOI51762.1"
                     /translation="MAQLILMPGKERSAMKQRHPWLFAGSVGRLEGRARPGDTVEVLA
                     DNLRPLGRAAYSPQSQIRARFWTFDADESVDDAFFKRRIAAAVARRDALPELRGQDGV
                     RLIHAESDGLPGVIADRYGDTVVVQLTSAGADKWRKAIVAGLVKATGCARVYERSDSE
                     VRALEGLEPTTGWLHGEAPTVPLAIMENGVRLAVDIAGGHKTGFYLDQRENRRLLGEL
                     AAGKEILNCFCYTGGFSLQALAGGAANVLSIDSSGPALAQARANLALNPQLPAE"
     gene            complement(894..1046)
                     /locus_tag="PLN02_05255"
     CDS             complement(894..1046)
                     /locus_tag="PLN02_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51763.1"
                     /translation="MAINRNRRNILERRCVSKTVKRLFKGDGKTTFKTMYAVECHQRN
                     RKPLGN"
     gene            complement(1141..2034)
                     /gene="xerC"
                     /locus_tag="PLN02_05260"
     CDS             complement(1141..2034)
                     /gene="xerC"
                     /locus_tag="PLN02_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOI51764.1"
                     /translation="MTPAAAVDAYLGELAGQRRLSPHTLSNYRRDLEKLLAAAGELPL
                     DQLLVHHIRRFVAGLHGGGLSGRSLARLLSAWRGFFAWLGARGLIRANPCEGIRAPKS
                     PRLLPKALSVDEAAQLMQPLADADDPFERRDAAMFELLYSSGLRLAELVGLDCSAVDG
                     IVHEGEVRVLGKRNKPRLVPVGTPARQAIAAWAAVRSALALPDEAALFVNTRGRRIGP
                     RSVEQRLAQRAVQLGLPQHVHPHMLRHSFASHVLQSSGDLRAVQEMLGHASIASTQVY
                     THLDFQHLASVYDKAHPRAKR"
     gene            complement(2031..2702)
                     /locus_tag="PLN02_05265"
     CDS             complement(2031..2702)
                     /locus_tag="PLN02_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOI51765.1"
                     /translation="MSALFPEDVAEYLKRNPGFFEQYADLMAQIFVPHPHGGRAVSLA
                     ERQMLTLREKNRASESKLAELIAFGEENDQISEKVHRLAVGLIAAETFQAVIHLLNFH
                     LRDDFSVPHVALRLWDKPAGIEDLPEFAAVSEELQVFAETLARPYCGSTAGFGTASWF
                     GEAAPHIRSQALIALRNGGGTIGMVALGSEETQRFYADMGTLYLERIGEMVSAALARV
                     TKSTL"
     gene            complement(2699..3529)
                     /gene="dapF"
                     /locus_tag="PLN02_05270"
     CDS             complement(2699..3529)
                     /gene="dapF"
                     /locus_tag="PLN02_05270"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOI51766.1"
                     /translation="MKLKFTKMHGLGNDFVVLDGVRQQFSLTPEQLRYLGDRHFGVGC
                     DQILVVEKPTQDGVDFRYRIFNADGGEVEQCGNGARCFVRFVHEQGLTDKREILVETM
                     KGVIAPRLEGDGNVTVDMGVPRFLPHEIPFLHDDDVVVYNLDVADETLEVSVVSMGNP
                     HAVQVVDSVDSAPVGEHGPLIESHARFPQRVNAGFMQVVDKHAIRLRVYERGAGETLA
                     CGTGACAAAVAGIRRGLLESPVRVSTRGGDLTIAWGGDGRPVLMTGPAQTVFSGEIDL
                     "
BASE COUNT          664 a         1193 c         1234 g          628 t
ORIGIN      
        1 gttccgccgg cagttgcgga ttgagcgcca ggttggcgcg ggcctgcgcc agggccggcc
       61 cggacgagtc gatcgacagc acattggcgg caccgccagc cagcgcctgc agcgaaaaac
      121 caccggtata gcaaaagcag ttgaggattt ccttgccggc ggccagttcg ccgagcaagc
      181 ggcggttctc gcgctggtcg aggtaaaagc cggtcttgtg gccaccggca atatcgaccg
      241 ccagccgcac accgttttcc atgatcgcca gcggcaccgt cggcgcctcg ccgtgcagcc
      301 agccggtggt cggctccagc ccttcgaggg cgcgcacttc ggaatccgag cgttcgtaga
      361 cgcgggcaca gccggtcgcc ttgaccaggc cggcgacgat cgccttgcgc cacttgtcgg
      421 cgccggccga agtcagctgg acgacgacgg tatcgccata acggtcggcg atgaccccgg
      481 gcaggccgtc ggattcggca tggatcaggc gcacgccgtc ctggccgcgc aactccggca
      541 acgcgtcgcg gcgggcgacg gcggcggcga tacggcgctt gaagaaggca tcgtccaccg
      601 attcgtcggc atcgaaggtc cagaagcggg cgcggatctg cgattgcggg ctgtaggcgg
      661 cgcgcccgag cggacgcagg ttgtcggcca gcacttcgac ggtgtcgccg ggccgggcgc
      721 gcccttcgag gcggccgacc gagccggcga acagccaggg gtggcgctgc ttcatcgccg
      781 agcgttcctt gccgggcatc aggatcaact gggccatatc tatttctcga tcggtaaatg
      841 caagaagccc cggttacctt gcgataaccg gggccgggaa tgcgtgttgt ggcttagttg
      901 cccagcggct tgcggttgcg ctgatggcac tcgacggcgt acatggtctt gaacgtcgtc
      961 ttgccgtcgc ccttgaacag gcgcttgacg gttttcgaaa cgcagcggcg ttccaggatg
     1021 ttgcggcggt tgcggttgat ggccatgact gactccggat tacggcaaaa ttttgaaaga
     1081 cgggcattat gccgggttga ccggacggcg gtcaattacc acttgatttg tttgccgttt
     1141 tcagcgcttg gcccgcggat gcgccttgtc gtagacgctg gccagatgct gaaagtcgag
     1201 gtgggtataa acctgggtcg aagcaatgct ggcgtgaccg agcatttcct gcaccgcgcg
     1261 caggtcgccg gacgactgca ggacatgcga agcgaaggaa tgacgcagca tgtgcggatg
     1321 gacgtgctgc ggcaggccga gttggacggc ccgctgcgcc agccgctgtt cgacgctgcg
     1381 cggcccgatg cggcggccgc gggtgttgac gaagagcgca gcttcatcgg gcaaggccag
     1441 cgcgctgcgg accgccgccc aggcggcgat cgcctgccgg gccggcgtac caaccggcac
     1501 caggcgcggt ttgttgcgtt tgccgaggac ccggacctcg ccctcatgca cgatgccgtc
     1561 gaccgccgaa caatcaagcc cgaccagttc ggcgaggcgc aggccggacg aataaagcag
     1621 ctcgaacatc gccgcgtcgc gccgctcgaa ggggtcgtcg gcgtcggcca gcggctgcat
     1681 cagttgcgcc gcttcgtcga ccgacagcgc cttcggcaac aggcgcggcg acttcggcgc
     1741 ccggatgcct tcgcagggat tggcccggat caagccacgc gccccgagcc aggcaaagaa
     1801 gccgcgccag gccgagagca ggcgggccag cgagcggccg gagaggccgc cgccatgcag
     1861 tccggcgacg aaacgccgga tgtgatggac cagcaactgg tcgagcggca gttcacccgc
     1921 cgcggccagc agtttttcca ggtcgcgacg atagttgctc agcgtgtgcg gcgacagccg
     1981 gcgctgcccg gcgagttcgc cgaggtaggc gtcgaccgcg gccgccggcg tcacagcgtg
     2041 ctcttggtga cccgcgccag cgccgccgag accatctcgc cgatgcgctc gaggtagagc
     2101 gtccccatgt cggcatagaa gcgctgggtt tcttcgctgc ccagagcgac catgccgatc
     2161 gtgccgccgc cgttgcgcag ggcgatcaac gcctgcgaac ggatgtgcgg cgccgcttcg
     2221 ccgaaccagg aggcggtacc gaagccggcg gtcgaaccgc agtaggggcg cgccagcgtc
     2281 tcggcaaaca cctgcagctc ctcgctgacc gcggcgaatt ccggcaggtc ttcgatgccg
     2341 gcgggcttgt cccacaggcg cagggcgacg tgcggcacgg aaaaatcgtc gcgcaggtgg
     2401 aagttcagca ggtgaatcac cgcctggaag gtttcggcgg cgatcagccc gaccgccagc
     2461 cgatgcacct tttcggaaat ctggtcgttc tcctcgccga aggcgatcag ttcggccagc
     2521 ttcgactcgc tggcgcggtt cttctcgcgc agcgtcagca tctgcctttc ggccagcgaa
     2581 acggcacgcc cgccatgcgg atggggcacg aagatctgcg ccatcagatc ggcgtactgc
     2641 tcgaagaaac cgggattgcg cttcaggtac tcggcgacgt cttcgggaaa cagggcgctc
     2701 ataaatcgat ttctccggaa aatacggtct gggcgggacc ggtcatcagc accggacggc
     2761 cgtcgccgcc ccaggcgatg gtcaggtcac caccacgcgt gctgacgcgc accggcgatt
     2821 cgagcaggcc gcgccggatg ccggcgactg cggcggcgca ggcgccggta ccgcaggcca
     2881 gggtttcgcc ggcgccgcgc tcatagaccc gcagcctgat cgcgtgcttg tcgacgacct
     2941 gcatgaaccc ggcgttgacc cgttgcggaa aacgcgcgtg cgattcgatc aatggcccat
     3001 gctcgccaac cggcgcgcta tccacgctgt caacgacctg cacggcatgc gggttgccca
     3061 tcgagacgac gctgacttcc agcgtctcgt cggcgacgtc gaggttgtag acgaccacgt
     3121 cgtcgtcgtg caggaaagga atctcgtgcg gcaggaaacg cggcacgccc atgtcgacgg
     3181 tgacgttgcc gtcgccctcg aggcgcgggg caatcacgcc cttcatcgtt tcgacgagga
     3241 tttcgcgctt gtcggtcagc ccctgctcgt gcacgaaacg cacgaagcag cgggcgccgt
     3301 tgccgcattg ctcgacctcg ccgccatcgg cgttgaagat gcgataacgg aaatcgacgc
     3361 cgtcctgcgt cggcttttcg acgaccagaa tctgatcgca accgacgccg aaatggcggt
     3421 cgccaagata gcgcaactgc tcaggggtca gggaaaactg ctggcgcacg ccgtcgagaa
     3481 cgacgaagtc gttgcccagg ccatgcatct tggtgaattt gagcttcacg aaccgctcca
     3541 ctattgatca agaaaaagaa taacagagac agttagttat ctcaaattcc taaagttttt
     3601 tctccatcac gaaatcgtcc atcacgtaac cgcgaccgat atcgtcgacc atcgtctcgc
     3661 gcacgctgaa gccatacttc atgtacgagt tgatcgcctt cacgttgcgt ttgttgacc
//
LOCUS       DAOSDX010000185         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8810_length_3697_cov_3.140033, whole genome shotgun sequence.
ACCESSION   DAOSDX010000185 DAOSDX010000000
VERSION     DAOSDX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8810_length_3697_cov_3.140033"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1598
                     /locus_tag="PLN02_05275"
     CDS             129..1598
                     /locus_tag="PLN02_05275"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HOI51767.1"
                     /translation="MSQVQRIHHFINGEFTAPPAARYFDKRSPVDGRVIAHIAEAGQA
                     EVDAAVTAARSALKGEWGKLTTDQRVDLLYGVANEITRRFDDFVAAEMADTGQPAHVM
                     TQVFIPRGAANFKVFADVIKNVAAESFRMATPDGKGALNYAIRNPKGVIGVISPWNAP
                     FLLMTWKVGPALACGNTVVVKPSEETPLTATLLGEVMNAVGIPKGVYNVINGFGPDSA
                     GAYLTQHPGVDAITFTGETRTGTAIMKAAAEGMRDVSFELGGKNAGIVFADCNFDAAV
                     DGIFRSAFLNTGQVCLGTERVYVERPIFERFVAALKAKVESVRYGRPEDHASTYGPLI
                     SQEHRDKVLSYYKKAIDEGATLVTGGGVPEMPAELAGGSWVQPTIWTGLPETAAVVRE
                     EIFGPCCHIRSFDSEDEVIELANANDYGLATTIWTENLSRAHRVAERVEVGVTWVNSW
                     FLRDLRTPFGGSKQSGIGREGGVHSLEFYTETRNVCIKL"
     gene            1631..2548
                     /locus_tag="PLN02_05280"
     CDS             1631..2548
                     /locus_tag="PLN02_05280"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HOI51768.1"
                     /translation="MKKIRCALIGSGNIGTDLIYKIQRSPVLEPVWMVGIDPESEGLA
                     RARAMGLKTTAAGVDGLLPYVQEDNIQIAFDATSAYVHAENSRKLNELGVMMIDLTPA
                     AIGPLCVPPVNLREHAEKVEMNVNMISCAGQATIPIVHAVSRIQSVAYGEIVASLASK
                     SIGPGTRANLDEFTYTTSNAIEVVGGARKGKALAIINPAEPPMLMRNTISCLTDETPD
                     EARITESVLAMIKEVQKYVPGYRLVNGPLFDGKRVTVFMEVAGLGDYLPKYAGNLDIM
                     TAAATRTAEMFAEEILAGSIQLKPVEVAA"
     gene            2545..3609
                     /gene="dmpG"
                     /locus_tag="PLN02_05285"
     CDS             2545..3609
                     /gene="dmpG"
                     /locus_tag="PLN02_05285"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HOI51769.1"
                     /translation="MSEVNLKGRQVIVHDMCLRDGMHAKREQMSVAQMVKIASALDDA
                     GVPYIQVTHGAGLGGNSLQHGFAPHSNEEYLSAVCSAVKRSKVSVLLLPGLGTMRELQ
                     SAYDCGARSVHVATHCTEADTSPQHIAYARKLGMDSTGFLMMAHLNTPEGLAQQGKLM
                     ESYGAQTVYITDSAGYMLPADVKARVGALRAVLKPETEIGFHGHHNMGMGIANSIAAI
                     EAGACRIDASVGGLGAGAGNTPLEAFVAVCERMGIETGCDLFKLMDMAEDIIFPLMDH
                     IVRVDRSSLTLGFAGVYSTFLLHANRAAERFGIPARDILVELGRKKMIGGQEDMIIDT
                     AMTMAKERGLLQDATAGVAS"
BASE COUNT          700 a         1304 c         1129 g          564 t
ORIGIN      
        1 ccttcctcac cgtcgtgacc tgatcgtcgc agccgggcgg cccccgccgc ccggcctgcc
       61 tcgccgaccg ccgacgcgca gtcaaggact gtgcgggcct cttgcacgcc aaaaccacga
      121 gtcttgccat gagccaagtc caacgcatcc accatttcat caacggcgaa ttcaccgcac
      181 cacccgccgc ccgctatttc gacaagcgtt cgccggtcga cggccgcgtc atcgcccaca
      241 tcgccgaagc cggccaggcc gaggtcgatg ccgccgtgac cgccgcccgc agcgcattga
      301 aaggcgaatg gggcaagctg accaccgacc agcgtgtgga cctgctctat ggcgtcgcca
      361 acgagatcac ccgtcgcttc gacgatttcg tcgccgccga aatggccgac accggccagc
      421 cggcgcacgt catgacgcag gttttcattc cgcgcggcgc cgccaacttc aaggtcttcg
      481 ctgacgtgat caagaacgtc gccgccgaat ccttccgcat ggcgacgccg gacggcaagg
      541 gcgcgctgaa ctacgcgatc cgcaacccga agggcgtgat cggcgtcatc tcgccgtgga
      601 acgcgccctt cctcttgatg acgtggaagg tcgggccggc gctggcctgc ggcaacaccg
      661 tggtggtcaa gccatccgag gaaacgccgc tcaccgcgac gctgctcggc gaggtaatga
      721 acgcggtcgg cattcccaag ggcgtctata acgtgatcaa cggcttcggc ccggattcgg
      781 ccggcgccta cctgacccag cacccgggcg tcgatgccat caccttcacc ggcgagacgc
      841 gcaccggcac ggcgatcatg aaggccgccg ccgaaggcat gcgcgacgtc tccttcgagc
      901 tgggcggcaa gaatgccggc atcgtcttcg ccgactgcaa tttcgacgcg gcggtcgacg
      961 gcatcttccg ctccgccttc ctcaataccg ggcaggtctg cctgggcacc gagcgcgtct
     1021 atgtcgagcg gccgattttt gaacgcttcg tcgccgcgct gaaagccaag gtcgagagcg
     1081 tccgctacgg ccggccggaa gaccacgcca gcacctacgg cccgctgatc agccaggagc
     1141 accgcgacaa ggtgctctcc tactacaaga aggcgatcga cgaaggcgcc acgctggtca
     1201 ccggcggcgg cgtgcccgag atgccggccg aactggccgg cggcagctgg gtgcagccga
     1261 ccatctggac cggcctgccg gaaactgcgg cggtcgtccg cgaagagatt ttcgggccgt
     1321 gctgccacat ccgctccttc gacagcgagg acgaggtcat cgagctggcc aacgccaacg
     1381 actacggcct ggcgacaacg atctggaccg agaatctgtc acgcgcccat cgcgtcgccg
     1441 agcgcgtcga ggtcggcgtc acctgggtga atagctggtt cctgcgcgat ctgcgcacgc
     1501 ccttcggcgg ttccaagcag tccggcatcg gccgcgaagg cggcgtccat tcgctggagt
     1561 tctacaccga aacccgcaac gtctgcatca agctctgacc ccctcagccc ccaaccccaa
     1621 agaggttcac atgaaaaaaa tacgctgtgc gctgatcggt tccggcaaca tcggcaccga
     1681 cctgatctat aagatccagc gcagtccggt cctcgaaccg gtgtggatgg tcggcatcga
     1741 ccccgaatcg gaaggcctcg cccgcgcccg cgcgatgggc ctgaaaacca ccgccgccgg
     1801 tgtcgacggc ctgctgccct acgtgcagga agacaacatc cagatcgctt tcgatgccac
     1861 ttcggcctac gtccacgccg agaactcgcg caagctgaac gagctgggcg tgatgatgat
     1921 cgacctgaca ccggccgcca tcggcccgct ctgcgtgccg ccggtcaacc tgcgcgaaca
     1981 cgcggaaaag gtcgagatga acgtcaacat gatctcttgc gccggccagg cgacgatccc
     2041 catcgtccat gccgtgtcgc gcatccagtc cgtggcctat ggcgaaatcg tcgccagtct
     2101 ggcgtcgaaa tcgatcggcc ccggcacccg cgccaatctc gacgaattca cctacaccac
     2161 gtcgaacgcc atcgaagtcg tcggcggtgc ccgcaagggc aaggcgctgg ccatcatcaa
     2221 cccggccgaa ccgccgatgc tgatgcgcaa caccatctcc tgcctgaccg acgagacacc
     2281 ggacgaagcc cgcatcaccg aatcggtgct ggcgatgatc aaggaagtgc agaagtacgt
     2341 gccgggttac cgcctggtca atggcccgct gttcgacggc aagcgcgtca ccgtcttcat
     2401 ggaagtggcc ggtctcggcg actacctgcc caagtacgcc ggcaacctgg acatcatgac
     2461 ggcggcggcg acgcgcaccg ccgaaatgtt cgccgaggaa atcctcgccg gcagcatcca
     2521 gctcaaaccc gtggaggtgg cagcatgagt gaagtgaatc tgaaaggccg ccaggtcatc
     2581 gtccacgaca tgtgcctgcg cgacggcatg cacgccaagc gcgagcagat gtcggtcgcg
     2641 cagatggtca agatcgccag cgcgctcgac gacgccggcg tgccctacat ccaggtcacg
     2701 cacggcgccg ggctgggcgg caattcgctg caacacggtt ttgcgccgca cagcaacgag
     2761 gaatacctga gcgccgtgtg cagcgcggtc aagcgcagca aggtctcggt cctgctgctg
     2821 cccgggctgg gcaccatgcg cgaactgcag tcggcctacg actgcggcgc gcgcagcgtg
     2881 cacgtcgcca cccactgcac cgaggccgat acctcgccgc agcacatcgc ctacgcccgc
     2941 aagctgggca tggacagtac cggcttcctg atgatggccc acctgaacac gccggaaggc
     3001 ctggcgcagc aaggcaagct gatggaatcc tacggcgcgc agacggtgta catcaccgac
     3061 tccgccggct acatgctgcc ggccgacgtc aaggcgcggg tgggggcgct gcgcgcggtg
     3121 ctcaagccgg aaaccgagat cggcttccat ggccaccaca acatgggcat gggcatcgcc
     3181 aactcgatcg ccgccatcga ggccggcgcc tgccgtatcg acgcttcggt cggcggcctc
     3241 ggtgctggcg ccggcaacac gccgctcgaa gccttcgtcg ccgtctgcga acggatgggc
     3301 atcgagaccg gctgcgacct gttcaagctg atggacatgg ccgaggacat catcttcccg
     3361 ctgatggacc acatcgtccg cgtcgaccgc tcgtcgctga cgctgggctt tgccggcgtc
     3421 tattccacct tcctgctgca cgccaaccgg gcggccgagc gcttcggcat cccggcgcgc
     3481 gacatcctgg tcgaactcgg ccgcaagaag atgatcggcg gccaggaaga catgatcatc
     3541 gacaccgcca tgaccatggc caaggaacgc ggcctgctcc aggacgcaac agcaggagtc
     3601 gcatcatgaa gccctacgct gaacgagaag cctgcgccat cctggtcggc gctaccggcg
     3661 cctttggcac ggcgatcacc cagcgcctgc tcgacgc
//
LOCUS       DAOSDX010000186         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8819_length_3693_cov_3.540407, whole genome shotgun sequence.
ACCESSION   DAOSDX010000186 DAOSDX010000000
VERSION     DAOSDX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8819_length_3693_cov_3.540407"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PLN02_05290"
     CDS             <1..1307
                     /locus_tag="PLN02_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51770.1"
                     /translation="LGLVFVRINCPVLGHDVTALDPAAAAHGAARQELEKLNLGLAMG
                     SNVMLYLDDIQHTSPEFLQKFIALADGTRRIEGVWKGEARTYDMRGKRFAVVMAGNPY
                     TESGDVFKIPDMLANRADIYNLGDVLDGREALFALSYIENSLTANPALLPLASRDPKD
                     VQLLVRLAEGEEIPGSAFSHPYSALEQEELVALMQRLFRIRDLLLKVNLAYIDSAAQK
                     DSYRTEPPFKLQGSYRNMTKLAGKVTAIMRDEEIDALLRDHYRGEAQTLTTGAEENLL
                     KLAELLGTPSVDEQARWTAIKEAFVRQRKLGGDDSDPGTRIAASLLDVARAVDGLKPD
                     SALADRLGDGLDRIEAGLRAVRPSVHVEPGDLSQFAGVVGDMVKAYEKVLLPLVTATY
                     HKIRLDHSMWDEMKRVSTYLDKLDKETGGNPAPEKPKPRSKP"
     gene            1304..2008
                     /locus_tag="PLN02_05295"
     CDS             1304..2008
                     /locus_tag="PLN02_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOI51771.1"
                     /translation="MSGAAISIEDILALLAGGIADGRLSDDEKRALAAALDAAALRDE
                     DLRRLRNHSFDLVRERLADPLQLALLKWLEGVVRSLDAQRLPSAQIVQEASFSPDSEC
                     LQTIQRCLRHARQRIDICVFTLSDDRIAEEVLAAHRRGVAVRIVTDNDKEYDAGSDIA
                     RLRDAGIAVVVDRTEAHMHHKFAIFDGEKLLNGSYNWTRSAGQYNEENLVLSNDRQLV
                     GEFSAQFEKLWRQLGG"
     gene            complement(2023..2694)
                     /locus_tag="PLN02_05300"
     CDS             complement(2023..2694)
                     /locus_tag="PLN02_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI51772.1"
                     /translation="MNRIAPTALYQEVAERLRQRIYAHELPPGTWIDEQKLAEQYGIS
                     RTPLREALKVLASEGLVELKPRRGCYVTEISRQDLDDIFPLIALLEGRCAAEAVKRAK
                     AADLTALRAIHEKLEAAARDARVDAFFEANQEFHRKIQELSGNRWLLSVIQDLRKVLK
                     RSRLHSLSLEGRLQQSLEEHRAILARMLAGDATGAERQMYEHLLSGREALARIDEQPG
                     GGKAA"
     gene            2862..>3693
                     /locus_tag="PLN02_05305"
     CDS             2862..>3693
                     /locus_tag="PLN02_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOI51773.1"
                     /translation="MSEKFFDSNNLDVWEKAAAKQSPGGDVNNLVWNTPEGLAVKALY
                     TKKDVDNLEFADTLPGVAPYLRGPQPTMYAVKPWTIRQYAGFSTAEASNAFYRKALAA
                     GGQGVSVAFDLATHRGYDSDNARVTGDVGKAGVAIDSVEDMKILFDGIPLDKISVSMT
                     MNGAVLPILAGYIVAAEEQGVKQEQLSGTIQNDILKEFMVRNTYIYPPKPSMKIIADI
                     FAYTAQNMPKFNSISISGYHIQEAGANQAIELAFTLADGMEYVRTGIASGMDVDTFAG
                     R"
BASE COUNT          709 a         1285 c         1121 g          578 t
ORIGIN      
        1 gtctcggcct ggttttcgtg cgcatcaact gcccggtcct cggccacgac gtcaccgccc
       61 tcgaccccgc tgctgccgcc cacggcgccg cccgccagga gctggagaag ctcaacctcg
      121 gtctggccat gggcagcaac gtgatgctct acctcgacga catccagcac accagcccgg
      181 agttcctgca gaagttcatc gccctggccg acggtacgcg gcgcatcgaa ggcgtctgga
      241 agggcgaggc gcgcacctac gacatgcgcg gcaagcgttt cgccgtggtc atggccggca
      301 acccctacac cgagtccggc gacgtcttca agatcccaga catgctggcc aaccgcgccg
      361 acatctacaa cctgggcgac gtcctcgacg gccgcgaagc gctgttcgcg ctgtcctaca
      421 tcgagaacag cctgaccgcc aacccggcgc tgctgccgct ggcttcgcgc gatccgaagg
      481 acgttcaact cctggtccgc ctcgccgagg gcgaggaaat ccccggcagc gccttcagcc
      541 atccctacag cgccctcgaa caggaggagc tggtcgcgct gatgcagcgc ctgttccgca
      601 tccgcgacct gctgctcaag gtcaacctgg cctacatcga ctcggcggcg cagaaggaca
      661 gctaccggac cgagccgccg ttcaagctgc agggcagcta ccgcaacatg accaaactcg
      721 ccggcaaggt caccgcgatc atgcgcgacg aggaaatcga cgccctgctg cgcgaccatt
      781 accgcggcga agcgcagacg ctgaccaccg gcgccgagga aaacctgctc aagctcgccg
      841 aactgctcgg caccccgagc gtcgatgaac aggcacggtg gaccgcgatc aaggaagcct
      901 tcgtccgcca gcgcaagctg ggcggcgacg acagcgaccc cggcacgcgc atcgccgcct
      961 cgctgctcga tgtcgcccgc gccgtcgacg gcctgaagcc cgacagcgcg ctcgccgacc
     1021 gcctgggcga cggtctcgac cgcatcgagg ccgggctgcg cgcggtcagg ccgagcgtcc
     1081 atgtcgagcc cggcgacctc tcgcaattcg ccggcgtcgt cggcgacatg gtcaaggcct
     1141 acgaaaaggt cctgctgccg ctggtcaccg cgacctacca caagatccgc ctcgaccact
     1201 cgatgtggga cgaaatgaag cgggtcagca cctacctcga caaactcgac aaggaaaccg
     1261 gcggcaaccc ggcgccggaa aaacccaaac cccggagcaa accatgagcg gcgcggcaat
     1321 cagcatcgag gacatcctgg ccttgctcgc cggcggcatc gccgacggcc gcctgagcga
     1381 cgacgagaaa cgcgcactgg ccgccgccct ggatgccgcc gcgctgcgcg acgaggacct
     1441 gcgccgcttg cgcaaccatt ccttcgatct ggtccgggaa cgccttgccg acccgctgca
     1501 actagcgctg ctgaaatggc tggaaggcgt cgtccgcagc cttgacgccc agcgcctgcc
     1561 gagcgcgcaa attgtccagg aagccagctt cagccccgac agcgagtgcc tgcaaacgat
     1621 ccagcgctgc ctgcgccacg cccgccagcg catcgacatc tgcgtgttca cgctgtccga
     1681 cgaccgcatc gccgaagaag tcctggccgc ccatcggcgc ggcgttgcgg tgcgcatcgt
     1741 caccgacaac gacaaggagt acgacgccgg cagcgacatc gcccgcctgc gcgatgccgg
     1801 gatcgccgtc gtcgtcgacc gcaccgaggc gcacatgcac cacaagttcg ccatcttcga
     1861 cggcgaaaag ctgctcaacg gcagctacaa ctggacgcgc agcgccggcc agtacaacga
     1921 ggaaaatctg gtactcagca acgaccggca actggtcggg gaattttcgg cgcagttcga
     1981 gaaactctgg cgccaactgg gcggctgaac gccgccaacg gctcaggcgg ccttgccgcc
     2041 cccaggttgc tcgtcaatcc tggccagcgc ctcgcgtccg gacagcaagt gctcgtacat
     2101 ctgccgctcg gcgccggtcg catcaccggc cagcatgcgg gcgaggatcg cccggtgctc
     2161 ttccagcgac tgctgcaggc ggccttcgag cgacagggaa tgcagacggg aacgcttcag
     2221 caccttgcgc aaatcctgga tcaccgacag cagccagcgg ttgccggaca actcctgaat
     2281 cttgcggtgg aattcctgat tggcctcgaa gaaggcatcg acgcgcgcgt cgcgcgccgc
     2341 cgcttcgagc ttctcgtgaa tcgcccgaag cgccgtcagg tccgccgcct tggcccgctt
     2401 cacggcttcc gccgcgcagc ggccctcgag gagggcaatc agcggaaaga tgtcgtcgag
     2461 gtcctggcgg gaaatctcgg tgacgtagca gccgcggcgc ggcttcagtt cgaccaggcc
     2521 ttccgacgcc agcaccttca gggcttcgcg caagggcgtg cgcgagatgc catactgctc
     2581 ggccagcttc tgttcgtcga tccaggtgcc tggcggcagt tcgtgggcgt agatgcgttg
     2641 tcgcagacgt tcggcgactt cctggtaaag cgcggtggga gcgatgcggt tcattgtctc
     2701 gagagttgtg agccggacag gccggtacgt cgccggcttg cctccgtaat tatggataaa
     2761 gtattatgta gggctgacca tgtcaagctg ggccctgaaa gcgtagaatt ttgaattctg
     2821 catcatcggc ccgaaaaaat cgaatcggcg agaggtgttt catgtctgaa aagttctttg
     2881 attccaacaa cctggatgtc tgggaaaagg ccgcggccaa acagtccccg ggtggcgatg
     2941 tcaacaacct ggtgtggaat acccccgaag gccttgccgt caaggcgctg tacacgaaga
     3001 aggatgtcga caacctcgag tttgccgaca ccctgccggg cgtcgcgccc tacctgcgcg
     3061 gcccgcagcc gacgatgtac gcggtcaagc cgtggaccat ccgccagtac gccggcttct
     3121 ccaccgccga agcctccaac gccttctacc gcaaggcgct cgccgccggc ggccagggcg
     3181 tttccgtggc cttcgacctg gccacgcacc gcggctatga ctcggacaac gcccgtgtca
     3241 ccggcgacgt cggcaaggcc ggcgtggcga tcgactcggt tgaggacatg aagatcctgt
     3301 tcgacggcat cccgctcgac aagatttccg tgtcgatgac catgaacggc gccgtcctgc
     3361 cgatcctcgc cggctacatc gtcgccgccg aggaacaggg cgtgaagcag gaacagttgt
     3421 ctggcacgat ccagaacgac atcctcaaag agttcatggt gcggaacact tacatctatc
     3481 cgcccaagcc gtcgatgaag atcatcgccg acatcttcgc ctacacggcg cagaacatgc
     3541 cgaagttcaa ctcgatctcg atttcgggtt accacatcca ggaagccggc gccaaccagg
     3601 ccatcgagct ggccttcacg ctggccgacg gcatggaata cgtgcgcacc ggcatcgcct
     3661 cgggcatgga cgtcgacacc ttcgccggcc gcc
//
LOCUS       DAOSDX010000187         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8845_length_3685_cov_3.379890, whole genome shotgun sequence.
ACCESSION   DAOSDX010000187 DAOSDX010000000
VERSION     DAOSDX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8845_length_3685_cov_3.379890"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PLN02_05310"
     CDS             <1..944
                     /locus_tag="PLN02_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOI51774.1"
                     /translation="SITQQAIQLGYAPRLQIQHTSSSEIGQIYLPAINWLLLAAIVAL
                     VAGFGSSSRLAAAYGIAVTGTMLITNLLAIAVAVRLWHWHPLRACLGALPFIIIDLGF
                     FLANSTKIPEGGWFPLAFGLAVFTLLTTWKRGRELLARRLADDTMELASFLAAIAGNG
                     PQRVPGTAVFLTTTPDNVPHALLHSLKHYKVLHEQVVILSVRVSEVPFVAAEERVTVR
                     RLAGDFSQVIVHYGFKDEPDIPQALALCAGQGLNIEMMETSFFLGRETLIPKFGSGMA
                     YWRALLFVALFRNASSAMAFFRIPSNRVIELGSQVVL"
     gene            complement(952..1980)
                     /locus_tag="PLN02_05315"
     CDS             complement(952..1980)
                     /locus_tag="PLN02_05315"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOI51775.1"
                     /translation="MNPATDDALAFACRMPKAELHLHIEGSLEPGLAFALARRNGITL
                     PYADEAALAAAYDFDSLQSFLDLYYACADVLRTAADFRDLMLAYLERAAADHVVHAEI
                     FFDPQTHTARGIPIGDVIEGLDAGIEEGRARWGISASLILCFLRHLSEDDGFATLAEA
                     EPWLERIAGFGLDSSEKGHPPAKFERLFARCRALGKPVVAHAGEEGPPTYVVEALDLL
                     GAARIDHGVRSVEDPALVDRLVREAVPLTVCPLSNLRLCVFPEMSRHTLPQLLAAGAR
                     VTVNSDDPAYFGGYLNDNIRALQAAFHFDKAAWYRIARNSFEASFASEAQKAAWIASL
                     DACLAESA"
     gene            complement(2050..>3369)
                     /locus_tag="PLN02_05320"
     CDS             complement(2050..>3369)
                     /locus_tag="PLN02_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51776.1"
                     /translation="ADIVSQYRGELLTIAGGLLLVLILYVRLLQTQRQLRQQNQAMSS
                     VIWGTGVGTWEWNVQTGETRFNERWAAILGYSLAELQPTTIDTWNAMLHPEDAQRSAE
                     QLRAHFAGDCEHYECEVRVRHKDGHWVWIMDRGRVTARDRDGKPLWMAGTHLEIGKRK
                     AAEEKLAHYQQDLERLVELRTRDLSLAKEAAEAASRAKTAFLANASHELRTPMHGILG
                     MLALVMRRTSDEKSLKHLQVAETSARRLLGLVSNVLDIARLEGDKLSLDAQPFDLGSV
                     GDSLQASVAEQIAAKGLKFTLELPSVLRRRTLSGDRVRLEQVLHNLLANALTYTSQGG
                     IDLLISPVAEDAAAIDIHFAVRDTGVGIPEKDQERIFLAFEQGDNSMTRQYGGSGLGL
                     AISHRLVRLMGGEMHVISQAGAGSIFSFTLRFPKAEAVEAMPLASPA"
     assembly_gap    3370..3379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3380..>3685)
                     /locus_tag="PLN02_05325"
     CDS             complement(<3380..>3685)
                     /locus_tag="PLN02_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOI51777.1"
                     /translation="EKLVAEGRLAENRLRIVNLQRLPSFPLGLSTRLYPEWPFAALYH
                     VDADLAGHVAAALLTLHDSPGLLAEMKVQGFVVPVDYAPVVDLLRSLRMPPFDVQHEV
                     "
BASE COUNT          563 a         1187 c         1291 g          634 t
ORIGIN      
        1 tttcgatcac ccagcaggcg atccagctcg gttacgcgcc gcgcctgcag atccagcaca
       61 cgtcgagcag tgagatcggc cagatctacc tgccggcgat caactggctg ctgctcgcgg
      121 cgatcgtcgc gctggtcgcc ggtttcggtt cctcgtcgcg gctggcggcg gcctacggca
      181 tcgccgtcac cggcaccatg ctgatcacca acctgctcgc catcgccgtc gccgtccgcc
      241 tctggcactg gcacccgctg cgcgcctgcc tgggcgccct gcccttcatc atcatcgacc
      301 tcggcttctt cctcgctaat tcgaccaaga ttccggaggg cggctggttc ccgctggctt
      361 tcgggctggc cgtgttcacc ctgctgacga cctggaagcg cggccgtgag ctgcttgccc
      421 gccggctggc cgacgacacc atggaactcg cctccttcct cgccgcgatt gccggcaacg
      481 gcccgcagcg ggtgccgggc acggcggtct tcctgaccac caccccggac aacgtgccgc
      541 acgcgctgct gcacagcctg aagcactaca aggtgctgca cgagcaggtg gtgatcctca
      601 gcgtccgggt cagcgaggtg cctttcgtcg ccgccgaaga acgcgtcacg gtgcgccgcc
      661 tggccggcga cttcagccag gtgatcgtcc attacggctt caaggatgag ccggacatcc
      721 cgcaggcgct ggcactgtgc gccgggcagg ggctgaacat cgaaatgatg gaaacctcgt
      781 tcttcctcgg ccgcgagacg ctgattccca agttcggctc gggcatggcc tactggcgcg
      841 ccctgctctt cgtcgcgctg ttccgcaacg cgagcagcgc catggccttc ttccggattc
      901 cgtcgaaccg ggtgatcgaa ctcggttcgc aggtggtgct ttagcctcgc ctcaggcgct
      961 ttccgccaga caggcgtcga ggctggcgat ccaggccgcc ttctgcgcct cgctagcgaa
     1021 gctcgcttcg aagctgttgc gggcgatgcg gtaccaggcg gccttgtcga aatggaaggc
     1081 ggcctgcagg gcgcggatgt tgtcgttgag gtagccgccg aaataggccg ggtcgtcgct
     1141 gttgacggtg acccgggcgc ccgccgcgag cagttgcggc agggtgtggc gggacatttc
     1201 cgggaagacg cagaggcgca ggttggagag cgggcagacg gtgagcggca cggcttcgcg
     1261 caccagccgg tcgaccagcg ccgggtcttc gaccgagcgc acgccgtggt cgatgcgcgc
     1321 cgcgccgagc aggtcgagcg cttcgacgac gtaggtcggc gggccttcct cgccggcatg
     1381 ggcgacgacc ggcttgccga gcgcccggca gcgggcgaac aggcgctcga acttggccgg
     1441 cgggtggccc ttttccgaag aatcgagacc gaagccggcg atccgctcga gccagggttc
     1501 ggcttcagcc agcgtggcga agccgtcgtc ctcggagagg tggcgcagga agcagaggat
     1561 caggctggcg cttatgcccc agcgcgcccg cccttcctcg atgccggcat caagcccctc
     1621 gatcacgtca ccgatcggaa tgccgcgcgc ggtgtgcgtc tgcggatcga agaagatttc
     1681 ggcgtggacg acgtggtcgg cggcggcgcg ttcgagatag gcgagcatca ggtcgcggaa
     1741 gtcggcggcc gtacgcagga cgtcggcgca ggcgtagtag aggtcgagga aggactgcag
     1801 gctgtcgaaa tcgtaggcgg cggctagcgc tgcctcgtcc gcatagggca aggtgatgcc
     1861 gttgcggcgg gcaagcgcga aggcgagccc gggttcgagc gagccttcga tatgcaggtg
     1921 gagttcggcc ttcggcatgc ggcaggcgaa ggccagcgcg tcatcggtag cgggattcat
     1981 cgtttcggct ccggcagtgg ggatcgccac atcctacccg aagcgcccgt cagcaggcca
     2041 gccggcggct caggccgggc tggcgagcgg catggcttcg actgcttcgg ctttcgggaa
     2101 acgcagcgtg aaggagaaga tgctgccggc gccggcctgg ctgatgacgt gcatctcgcc
     2161 gcccatcagg cgcacgaggc ggtggctgat ggccaggccg aggccgctgc cgccgtactg
     2221 ccgggtcatc gagttgtcgc cctgctcaaa ggccaggaag atgcgctcct ggtccttttc
     2281 cgggatgccg acgccggtgt cgcggaccgc gaagtggatg tcgatcgccg ccgcatcctc
     2341 ggccaccggg ctgatcagca ggtcgatgcc gccctggctg gtgtaggtca gcgcgttggc
     2401 gagcaggttg tggagcacct gctccaggcg gacccggtcg ccggacaggg tccgccgccg
     2461 caagacggat ggcagttcga gggtgaactt gagacccttg gcggcgatct gctcggcgac
     2521 cgatgcctgc aggctgtcgc cgacgctgcc gagatcgaaa ggctgcgcgt cgagactgag
     2581 cttgtctccc tccaggcggg cgatgtcgag cacgttgctg accaggccga gcaggcggcg
     2641 ggcagaggtt tcggcgacct gcaggtgttt caggctcttt tcgtcgctgg tgcggcgcat
     2701 gaccagcgcc agcatgccca ggatgccgtg catcggcgtc cgcaattcgt ggctggcatt
     2761 ggccaggaag gcggtcttgg cacggctggc ggcttccgcc gcttccttgg cgagcgacag
     2821 gtcgcgggtg cgcagctcga ccaggcgttc gaggtcctgc tggtaatgtg cgagtttttc
     2881 ctcggccgcc ttgcgcttgc cgatttcgag atgggtgccg gccatccaca gcggcttgcc
     2941 gtcgcggtcg cgggcggtga cccggccgcg gtccatgatc cagacccagt gcccgtcctt
     3001 gtggcggacc cggacctcgc attcgtagtg ttcgcagtcg ccggcgaaat gcgcgcgcaa
     3061 ctgttccgcc gagcgctgcg cgtcttccgg atgcaacatg gcgttccagg tgtcgatcgt
     3121 ggtcggctgc aactcggcca gggaatagcc gaggatcgcc gcccagcgct cgttgaagcg
     3181 ggtttcgccg gtctgcacgt tccattccca ggtgccaacg ccggtgcccc agatgacgct
     3241 ggacatcgcc tggttctgtt ggcgcagttg ccgctgcgtc tgcagcaggc ggacgtagag
     3301 gatcaggacc agtaacaggc cgccggcgat ggtcagcagt tcgccgcggt actggctgac
     3361 gatgtcggcn nnnnnnnnnc acttcgtgct gcacgtcgaa gggcggcatg cgcaggctgc
     3421 gcagcagatc gacgaccggc gcgtaatcga cgggaacgac gaagccctgg accttcatct
     3481 ccgccaggag gccgggcgag tcatgcaggg tcagcaaggc cgccgcgaca tgccccgcaa
     3541 gatcggcatc gacgtgatac agcgcggcaa acggccattc cggatagagg cgcgtcgaca
     3601 agccaagcgg aaaggacggc aggcgctgga gattgacgat ccgtagccga ttctcggcga
     3661 gccggccttc ggcaaccagt ttctc
//
LOCUS       DAOSDX010000188         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8870_length_3676_cov_2.612262, whole genome shotgun sequence.
ACCESSION   DAOSDX010000188 DAOSDX010000000
VERSION     DAOSDX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8870_length_3676_cov_2.612262"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLN02_05330"
     CDS             <1..1044
                     /locus_tag="PLN02_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOI51778.1"
                     /translation="TKGVPYKVYGGLRFFERQEVKHALAYLRLLGNPDGDTAFLRVVN
                     FPTRGIGARSLENLQAAAHQMNSSLYNAAASLTGKAGQSVGAFIRLVETLRQETEKLP
                     LPEIIEHVVEKSGLAQHYRLEKEGQERLENLDELINAAASFVDDEGAVGEGGALASFL
                     AHASLESGEHQAGEGQEAVQLMSVHAAKGLEFDVVFISGLEQGLFPHENAVSEGRDGL
                     EEERRLMYVAVTRARQRLYLSCAQTRMLHGQTRYCTASSFFDEIPEELLLKLNKKAAA
                     PAPTWGGYAAASSEPVAGGLRIGQNVEHAKFGFGVIVATEGRGADARVQVNFGGGAGM
                     KWLALEYAKLTPV"
     gene            1134..2462
                     /locus_tag="PLN02_05335"
     CDS             1134..2462
                     /locus_tag="PLN02_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOI51779.1"
                     /translation="MMAKIKAGLISLRDLFATAWWIFLIAGIGFVVAFQFVEPAPPRH
                     VVMTSGGESGAYYQFAKRYAAILARSGITLEVRTSEGSVQNLERLRSGEAQIGFVQGG
                     VIEPVDESRIDEEPENWGLLSLGSMFYEPVWVFYQGETIERLSELRGKRIAIGQEGSG
                     IRQLAQRLLEDNDVDFRQNTLPISGLDAAEELQQGRIDAAFIIAAEHAPVVQVLLRSP
                     GIKLMNFSQSFAYERRYPFLTRLTMPQGVADLVLDIPPADTRVLAPTASLVVRDDLHP
                     ALQNLLLQAARQIHGKPGFFQTANEFPSYMDGMLPLSSEAERFFKSGPPFLQRYLPFW
                     LAVLVDRLIVLLVPVIALLIPLLRVAPALYSWRVKSKVFRCYGELKFLEDDLKNHYDP
                     ARLAEYRQRLDALDEEAAELHVPLGFTDLVYTLREHVNLVRSILDKKERE"
     gene            2459..3097
                     /locus_tag="PLN02_05340"
     CDS             2459..3097
                     /locus_tag="PLN02_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOI51780.1"
                     /translation="MKGFLVANPKGGSGKSTLATNLAGYFASRGHDVMLGDIDRQQSA
                     REWLATRPFALPTIETWEIGPDRIARPPKGTSHVVLDTPAGLHGKQLDKVLKLSTRVI
                     VPVQPSLFDMRATRHFLEALLDEKAVRKGKADVAVIGMRVDGRTRAAAELERFFASFE
                     LPVLAYLRDTQVYVQAAAAGLTLFDLPRSRAERDLEQWQAIIEWVEKPGQAG"
     gene            3099..3326
                     /locus_tag="PLN02_05345"
     CDS             3099..3326
                     /locus_tag="PLN02_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HOI51781.1"
                     /translation="MLKYLLLLALGALLWWHWRKGRDGAVPPSETPRPVERMVRCAHC
                     GVHLPESDALADGARHYCNAAHRDAARRAGG"
     gene            3268..>3676
                     /locus_tag="PLN02_05350"
     CDS             3268..>3676
                     /locus_tag="PLN02_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51782.1"
                     /translation="MLVTTATRRIATPPGAPGAEVQGWLNDTWVPNWRSFQYFVLYRL
                     LLGSLALLALLFPGDWNLRLNMMPSLLTFSLVGAHLVCTAGGLALARFWQQHFNFQLS
                     FQVIVDIVVLAALMLIAGGVSSGFGLILLISLAA"
BASE COUNT          584 a         1232 c         1217 g          643 t
ORIGIN      
        1 accaagggcg tgccctacaa ggtctatggt ggcctgcgct tcttcgagcg ccaggaagtc
       61 aagcacgcgc tcgcctacct gcgcctgctg ggcaacccgg acggcgacac cgccttcctg
      121 cgcgtcgtga acttcccgac ccgcggcatc ggcgcccgtt cgctggaaaa cctgcaggcc
      181 gccgcgcacc agatgaactc cagcctctac aacgccgccg cttcgctcac cggcaaggcc
      241 ggccagagcg tcggcgcctt catccggctg gtcgagacgc tgcgccagga gaccgagaag
      301 ctgccgttgc ccgagatcat cgagcatgtc gtcgagaagt ccggcctggc ccagcattac
      361 cggctggaga aggaaggcca ggagcgcctg gagaacctcg acgaactgat caacgccgcc
      421 gccagcttcg tcgatgacga aggggcggtc ggcgagggcg gcgcgctcgc ttccttcctc
      481 gcccacgcct cgctcgaatc cggcgagcac caggccggtg agggccagga agcggtccag
      541 ctgatgtcgg tgcacgcggc caaggggctg gagttcgacg tcgtcttcat cagcggcctc
      601 gagcagggcc tgttcccgca cgagaacgcg gtcagcgagg ggcgcgacgg cctggaggaa
      661 gagcgccggc tgatgtacgt cgcggtcacc cgcgcccgtc agcggcttta cctgtcctgc
      721 gcgcagacgc ggatgctgca cgggcagacg cgctattgca cggcctcgtc ctttttcgac
      781 gagattccgg aggaactgct gctcaagctc aacaagaagg ccgcggcacc ggcgccgacc
      841 tggggcggct acgccgccgc cagcagcgag ccggtcgctg gcggcctgcg catcgggcag
      901 aacgtcgagc atgccaagtt cggtttcggc gttatcgtcg ccaccgaagg gcgcggtgcc
      961 gacgcgcggg tccaggtcaa cttcggcggc ggcgccggca tgaaatggct ggcgctggag
     1021 tacgccaagc tgacgccggt atgacgtcgt cagcgccggc cgcgcgctag atcgcaagcg
     1081 ctgctagtgt taccctcgca gtctcgacga cgacagggtt ttcccgagca cgcatgatgg
     1141 cgaaaatcaa ggctggactt atctcgctgc gcgacctgtt cgcgacggcc tggtggatct
     1201 ttttgatcgc cggcatcggt ttcgtcgtcg ccttccagtt cgtcgagccg gcgccgccgc
     1261 gccacgtggt gatgaccagc ggcggcgaga gcggcgccta ctaccagttt gccaagcgct
     1321 acgcggcgat cctggcgcgc tcggggatca cgctggaggt gcggacctcc gagggctcgg
     1381 tgcagaacct tgaaaggctc aggagcggcg aagcgcagat cggcttcgtc cagggcggcg
     1441 tcatcgagcc ggtagacgag agccggatcg acgaggaacc ggaaaactgg ggcctgctgt
     1501 cgttgggcag catgttctac gagccggtct gggtcttcta tcagggtgag acgatcgagc
     1561 gcctgagcga gttgcgcggc aagcgcatcg ccatcggcca ggaaggctcc ggcatccggc
     1621 aactggcgca gcgcctgctg gaggacaacg atgtcgattt tcgccagaac acgctgccga
     1681 tttccgggct ggatgccgcc gaggagttgc agcaagggcg catcgatgcc gccttcatca
     1741 tcgccgccga gcatgcgccg gtggtccagg tgctgttgcg ttcgcccggc atcaagctga
     1801 tgaatttttc ccagtccttc gcctacgagc ggcgttatcc cttcctgacc cggctcacca
     1861 tgccgcaggg ggtggccgat ctggtcctcg acatccctcc ggccgacacc cgggtgctgg
     1921 cgccgaccgc cagcctggtg gtgcgcgacg acctgcatcc ggcactgcag aacctgctgc
     1981 tgcaggcggc gcggcagatc cacggcaagc cgggtttctt ccagacggcc aacgagtttc
     2041 cctcgtacat ggacggcatg ctgccgctgt cgtcggaggc cgagcgtttc ttcaagtccg
     2101 gtccgccttt cctgcagcgc tacctgcctt tctggctggc cgttctggtc gaccggctga
     2161 tcgtcctgct ggtgccggtg atcgccctgc tcatcccgct gttgcgggtg gcgccggcgc
     2221 tctatagctg gcgggtgaaa tccaaggtct tccgttgcta cggcgagctg aagttcctcg
     2281 aggacgacct gaagaaccat tatgacccgg cgcgtctggc ggaataccgg cagcggctcg
     2341 acgcgctcga cgaagaggcg gccgaactgc atgtgccgct cggtttcacc gatctggtct
     2401 atacgctgcg cgagcacgtc aacctggtac gcagcatcct cgacaagaag gaaagggaat
     2461 gaaaggcttc ctcgtcgcca atcccaaggg cggttccggc aagagcacgc tggccaccaa
     2521 cctggccggc tatttcgcca gtcgcggcca tgatgtgatg ctcggcgaca tcgaccgcca
     2581 gcagtcggcg cgcgaatggc tggcgacgcg cccgttcgca ttgccgacga tagaaacctg
     2641 ggaaatcggt cccgatcgca tcgcgcggcc gcccaagggc acctcgcacg tcgtcctcga
     2701 taccccggcc ggcctgcacg gcaaacagct cgacaaggtg ctgaagctgt cgacgcgggt
     2761 gatcgtgccg gtccagccct cgctgttcga catgcgcgcc acccgccact tcctcgaagc
     2821 cctgctcgac gagaaggccg tgcgcaaggg caaggccgac gtcgccgtga tcggcatgcg
     2881 ggtcgatgga cggacgcgcg ccgccgccga actggagcgt ttcttcgcca gcttcgagct
     2941 gccggtgctg gcctacctgc gcgacaccca ggtctatgtc caggccgcgg ccgccgggct
     3001 gaccctgttc gacctgccgc gctcgcgcgc cgagcgcgac ctcgagcaat ggcaggcgat
     3061 catcgagtgg gtcgaaaagc ccggccaggc cgggtgaaat gctgaaatac ctgctgctgc
     3121 tcgcgctcgg cgcgcttctc tggtggcact ggcgcaaggg ccgggatggc gccgtgccgc
     3181 catcggagac gccacggccg gtcgagcgca tggtgcgttg cgcccattgc ggcgtacatc
     3241 tgccggaaag cgacgctctg gccgacggtg ctcgtcacta ctgcaacgcg gcgcatcgcg
     3301 acgccgcccg gcgcgccggg ggctgaggtg cagggctggc tgaacgacac ctgggtgccg
     3361 aactggcgct ccttccagta tttcgtcctc tatcgcctgc tgctcggcag tctggcgctg
     3421 ctggcgctgc tttttcccgg cgactggaac ctgcgcctca acatgatgcc ctcgctgctc
     3481 accttctcgc tggtcggcgc gcatctggta tgtaccgccg gcggtctggc gctggcgcgc
     3541 ttctggcagc aacacttcaa cttccagctt tcgttccagg tcatcgtcga catcgtcgtc
     3601 ctcgccgcct tgatgctgat cgccggcggc gtcagcagcg gcttcggctt gatcctgctg
     3661 atctctctgg cggcgg
//
LOCUS       DAOSDX010000189         3664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8899_length_3664_cov_3.635079, whole genome shotgun sequence.
ACCESSION   DAOSDX010000189 DAOSDX010000000
VERSION     DAOSDX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8899_length_3664_cov_3.635079"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLN02_05355"
     CDS             complement(<1..555)
                     /locus_tag="PLN02_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI51783.1"
                     /translation="MKRKAQKIKLPASFRTLPQTRSVKKTSIALGISTTMLAPVVAFA
                     AEPASEAEMAPVTVVERVIDHNPYAEPDAPYKARVSGDERRKRPLAETPANISVLTKT
                     QIEDSGYTDLRDILDAQPGITLGTGENGNAFGDRYIIRGQEARSDVFVDGLRDPGMTI
                     RESFAVEQVEISKGPSSSFAGRGTA"
     gene            complement(768..1106)
                     /locus_tag="PLN02_05360"
     CDS             complement(768..1106)
                     /locus_tag="PLN02_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51784.1"
                     /translation="MTAPSETDCLLLARALPVADLPVIDGLVDPARLPALLRCFTGVY
                     EAEYAPPSELLARHGEPPAIRAAIMAHAAGTGSEPLLLVRFSHARLADLLAILLQQGF
                     AGFSPVCRLS"
     gene            complement(1115..1321)
                     /locus_tag="PLN02_05365"
     CDS             complement(1115..1321)
                     /locus_tag="PLN02_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOI51785.1"
                     /translation="MENTEIRIGGMSCQGCVKNITGVLGGLPGVASAEVSLEAAQARV
                     VYDPQQISRAELLGAIEDAGFDAE"
     gene            complement(1325..3007)
                     /locus_tag="PLN02_05370"
     CDS             complement(1325..3007)
                     /locus_tag="PLN02_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI51786.1"
                     /translation="MVKRETDLFRATMDAAQVGIFVMARGRFLYVNPYMRSLFGYAEA
                     DFIERLRPYDLVVAEQHERIAEQLARRPGGGAVETTEFTGRRQDGSLFTMSVLGMPSR
                     FDGRVVAVGTVIDISDRKQAEQRIRELADFDALTGLPNRRLLHDRIEQMLAAAERDKH
                     GAALLFLDIDHFKRVNDSLGHSVGDDLLCALAQRYVKVVRRIDTLARLGGDEFIILLP
                     EVNAAAAADVARRLLEIAASPFSVGGHALTVTSSIGIALYPQDGKDIDTLLKNADTAM
                     YKAKELGRNTFQFYSSEMNMATLERLMMESNLRQALTEEQFVLHYQPLVSLQNGEIVG
                     VEALIRWLHPTLGTIMPDRFIAVAEDSGMINPIGDWVLFEACRQAQAWIDEGLQLRTM
                     AVNVAPVQFRQAGFVEMVAGALSATGLVADSLELELTERTVMHDAEFTLGTLSALKRM
                     GVSLSLDDFGTGYSSLAYLKRFPVGKLKIDRSFVMDLESDADDQAIASTIVSMGRSLR
                     LTVLAEGVETAEQLALLRKMGCGMVQGFHYSRPLAAAEMTELLRRQPFLHRD"
     gene            complement(3013..>3664)
                     /locus_tag="PLN02_05375"
     CDS             complement(3013..>3664)
                     /locus_tag="PLN02_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOI51787.1"
                     /translation="VAALQDAGKTVIALAEIDADEKVTHLLALIAIADALRPTSREAV
                     ARLRQRGIRVVMLTGDNPATAAAIAAQAGIDEFRAGILPGDKAAAVNALKADGGLVAM
                     VGDGINDAPALAAADVSFAIGAGSDAAIEAADLTLIRNDLLGVADAIALSAATLGKIR
                     QNLFFAFIYNVLGIPLAAFGFLSPVVAGAAMAMSSVSVVSNSLLLRRWRPEAGRRR"
BASE COUNT          662 a         1182 c         1206 g          614 t
ORIGIN      
        1 agcggtgccg cgaccggcga agctggaact cggccccttg gagatttcca cctgttcgac
       61 ggcgaagctc tcgcggatgg tcatgccagg gtcgcgcaaa ccatcgacga agacatcgct
      121 gcgcgcttcc tggccacgga tgatgtagcg atcgccgaag gcgttgccgt tctcgccggt
      181 accgagcgtg atccccggtt gcgcgtcgag gatgtcgcgc agatcggtgt agcccgaatc
      241 ctcgatctgc gtcttggtca gcacgctgat gttggccggc gtctcggcca acggccgctt
      301 gcgccgctcg tcgccggaga cgcgcgcctt gtacggggca tccggttcgg cataggggtt
      361 gtggtcgatg acgcgttcga cgacggtcac cggcgccatt tcggcttcgc tcgccggttc
      421 ggcggcaaac gccacgaccg gcgccagcat cgtcgtcgaa attcccagcg caatgctggt
      481 cttcttcacc gaacgggtct ggggcaaggt ccggaaactg gccggcagct tgattttctg
      541 agcttttctt ttcactctcg tatcctggat cgatgccgca gcgggaagtc cggacgagag
      601 ctgcctgcag gaggaagcgg ccaggacgtc ccaacggcaa aaggttgggg aaaagctggc
      661 tggcttcagg ctggcttgct taatcgcgga tgcaaatcta tcgcatttgc agttcagtta
      721 caagtggtaa cagagtcgta aaacagcatg cacaggaatt ccaggaatca ggacaggcga
      781 caaaccgggg aaaacccggc aaacccctgc tgcaggagga tggccagcag atcggcaagg
      841 cgggcgtgcg agaagcgcac gaggagcaac ggctcgctgc cggtgccggc agcgtgcgcc
      901 atgatcgccg ccctgatcgc ggggggctcg ccatggcgcg ccagcagttc ggacggcggg
      961 gcgtactcgg cctcatagac cccggtgaaa cagcgcaaca gcgccggcag gcgggccgga
     1021 tcgacaaggc cgtcaatcac cggcaggtcg gccacgggca aggcccgggc aagcaacaga
     1081 cagtcggtct ccgatggcgc cgtcatcggc ggaattattc ggcgtcgaaa ccagcgtctt
     1141 cgatcgcgcc aagcagttcg gcccggctga tttgctgcgg atcgtagacg acgcgcgcct
     1201 gcgccgcttc cagcgagacc tcggccgagg cgacgccggg caggccgccg aggacgccgg
     1261 tgatgttctt gacgcagccc tggcagctca tgccgccgat ccggatttcg gtgttttcca
     1321 tgtttcagtc cctgtggaga aaaggctggc ggcgcagcaa ttcggtcatc tcggcggcgg
     1381 ccagcggtcg gctgtagtga aagccctgca ccatgccgca acccatcttg cgcagcaaag
     1441 ccagttgctc ggcagtttcc acgccttcgg ccagtaccgt caagcgcagg ctgcgtccca
     1501 tgctgacgat ggtcgaagcg atcgcctggt cgtcggcgtc gctttccagg tccatgacga
     1561 aggaacggtc gatcttcagc ttgccgaccg ggaaacgctt caggtaggca agcgaggaat
     1621 agccggtacc gaaatcgtcg agcgacagac tgacgcccat gcgcttcaac gccgacaggg
     1681 tgccaagggt gaattcggca tcgtgcatca cggtgcgctc ggtcagttcg agttccagag
     1741 aatcggcgac caacccggtg gcgctcagag caccggcgac catctcgacg aaaccagcct
     1801 ggcggaactg caccggcgcc acattgaccg ccatcgtccg cagttgcaga ccttcgtcta
     1861 tccaggcctg ggcctgacga caggcttcga acaggaccca gtcgccgatc gggttaatca
     1921 tcccgctatc ctcggcaacg gcgatgaagc gatcgggcat gatcgtgccc agggtcggat
     1981 gcaaccagcg gatcagcgcc tcgacgccga cgatctctcc gttctgcaga ctgaccagcg
     2041 gctgatagtg aagaacgaac tgctcctcgg tcaaggcctg gcgcaggtta ctctccatca
     2101 tcaggcgctc aagggtcgcc atgttcattt ccgaggaata gaactggaag gtgttgcgtc
     2161 ccagttcctt ggccttgtac atcgccgtgt ctgcattctt gagcagggtg tcgatgtcct
     2221 tgccgtcctg cggatagagg gcgatgccga tcgacgaggt gacggtcagc gcgtggccgc
     2281 cgaccgaaaa cggcgaggcg gcgatctcca gcagccggcg ggcgacgtcg gccgccgccg
     2341 cggcattcac ctcgggcagc aggatgatga actcgtcacc gccgaggcgg gccagtgtgt
     2401 cgatccgccg caccaccttg acgtagcgct gcgccagcgc gcacagcagg tcgtcaccga
     2461 cgctgtggcc gagcgagtcg ttgacccgct tgaaatggtc gatgtcgaga aagagcagcg
     2521 ccgcgccgtg cttgtcgcgc tcggcggcgg caagcatctg ctcgatccgg tcgtgcagca
     2581 ggcggcggtt gggcaggccg gtcagggcgt cgaaatcggc cagctcgcgg atgcgctgtt
     2641 cggcctgctt gcggtcgctg atgtcgatga cggtgccgac ggcgaccacc ctgccatcga
     2701 agcgcgaagg catgccgagc accgacatgg tgaacagcga gccgtcctgg cgccgaccgg
     2761 tgaactcggt ggtttcgaca gcgccgccgc ccggtcgcct ggccagttgt tcggcgatcc
     2821 tttcgtgctg ctcggcaacg accagatcat agggccggag gcgctcgatg aaatccgcct
     2881 cggcgtagcc gaacaagctg cgcatgtaag gattgacgta gaggaagcgc ccccgcgcca
     2941 tgacgaagat gccgacctgc gccgcatcca tcgtcgcgcg aaagaggtcg gtttcccgct
     3001 tgaccatcac cgtcaacgcc gccgaccggc ttccggtcgc cagcgccgca gcagcagcga
     3061 attggaaacc accgacaccg aactcatcgc catcgccgcc cccgccacca ccgggctgag
     3121 gaagccgaaa gccgccagcg ggatgccgag aacgttgtag atgaaggcaa agaacaggtt
     3181 ctgccggatc ttgcccagcg ttgccgcgga aagtgcgatg gcgtcggcga cgccaagcag
     3241 gtcgttgcgg atcagcgtca ggtcggccgc ctcgatcgcc gcgtcggaac cggcgccgat
     3301 cgcgaaactg acgtcggccg cagccagcgc cggcgcatcg ttgatgccgt caccgaccat
     3361 cgccaccagc ccgccgtccg ccttcagcgc gttcaccgcg gccgccttgt cgcccggcag
     3421 gatgccggcg cggaattcgt cgatcccggc ctgggcggcg atcgccgccg ccgtcgccgg
     3481 attgtcgccg gtcagcatga ccacccggat gccgcgctgg cgcaggcggg ccaccgcctc
     3541 gcgcgaggtc gggcgcagcg catcggcgat ggcgatcaag gccaggagat gcgtgacttt
     3601 ttcgtcggcg tcgatctcgg ccagcgcgat caccgtcttg ccggcgtcct gcagcgccgc
     3661 gacc
//
LOCUS       DAOSDX010000190         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8941_length_3650_cov_3.572740, whole genome shotgun sequence.
ACCESSION   DAOSDX010000190 DAOSDX010000000
VERSION     DAOSDX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8941_length_3650_cov_3.572740"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..985
                     /locus_tag="PLN02_05380"
     CDS             62..985
                     /locus_tag="PLN02_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI51788.1"
                     /translation="MKFSLRQLEIFVAIARSENVSLAAGRLALSQSAASSALVELERA
                     YDCPLFDRVGKRLHLNSSGRGLLALAESLLAQAGEVDDYLGGGRPGPLAVGATLTIGN
                     YLATLVVVDYMRRYPDSPVSLQVANSETVAGRLLRGDCDFGLIEGEVAHPDLLVEPWL
                     ADELVVFCAPGHALAGRTAVDSATLGEQSWVLREPGSGTRSQFDRCIGSRLPAFRLAL
                     ELEHTEAIKRAVESGIGLGCLSRLALREAFRRGSLVELPTPQFDLRRHFYFVRQRSRH
                     PGLAGRLFREACLAVGGGAADTAGLQLPFIP"
     gene            1227..2510
                     /locus_tag="PLN02_05385"
     CDS             1227..2510
                     /locus_tag="PLN02_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOI51789.1"
                     /translation="MEIWILVFLILGCGLLAMAEMAIGASRNTHLAIRAEQGKAAAAV
                     VIGFRQNASRLLGTTQLGITALAMLSGVYGEALWVPRVEAWLLGFGLTAGPAYALALA
                     LVVIVITFFSLVFGEVIPKRLALAHPEAVAEALSGLIAFLIRLTRPLVSVVSKTADWL
                     LQFFPLKGSAEATAADEIRFLIEAGRKHGDLDQTESEILGNVFRLDNRKVAAIMTPAA
                     DIACLDLSESHETNIALLRERAVSRYLVSKGGLGNALGFVDSGDLLRELLSERELDFG
                     KLALQPLQYVPGSLSLIGLLEFFKANQTQIALVSNEFGATEGLVTLSDLMGTVVGDVL
                     SGAVESPLAIQRNDGSWLLDGLLPIDEMKQLLGIGELPEEELGNYHTVGGFVIVQLGR
                     IPRKTEAFDWAGWHFEVMDMEKNRVDEVLATRLSD"
     gene            2607..3626
                     /locus_tag="PLN02_05390"
     CDS             2607..3626
                     /locus_tag="PLN02_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI51790.1"
                     /translation="MKHSVIRRALLASALFAGLAGAGLAQAQTTLLNVSYDPTRELYV
                     EFNNAFAKHWKATTGQDVSVKQSHGGSGKQGRAVIDGLEADVVTLALAYDIDAISQAS
                     KGKLLPANWQQRLPHNSSPYTSTIVFLVRKGNPKGIKDWDDLVKPGVEVITPNPKTSG
                     GARWNYLAAWGYALKKYGSEAKAQAFVKDLLQHVPVLDSGARGSTNTFVQRGIGDVLL
                     AWENEAFLSINELGPDKFEIVVPSLSILAEPPVTVVNGVTEKRGTSKLATAYLEYLYS
                     AEGQKIAAKNYYRPIKPELADKKDLARFPKLNLIKIDELGGWQAAQKKHFADGGTFDQ
                     VYGKK"
BASE COUNT          597 a         1213 c         1219 g          621 t
ORIGIN      
        1 aggcttggtg gctttgaaac aaaagcggat aatttttcta ttcgttattg atgaaatcga
       61 tatgaagttc agcctgcgcc agctcgaaat cttcgtcgcc atcgcccgca gcgaaaacgt
      121 ctcgctcgcc gccgggcggt tggcgttgtc gcagtcggca gcgagcagtg cgctggtcga
      181 gctggagcgg gcctacgact gtccgctgtt cgaccgcgtc ggcaagcgcc tgcacctgaa
      241 cagcagcggc cgcggcctgc tggcactggc cgagtcgctg ctcgcccagg ccggcgaggt
      301 tgacgactat ctcggcggcg gccgtcccgg gccgctggcg gtgggggcga cgctgaccat
      361 cggcaattac ctggcgacgc tggtcgtcgt cgattacatg cgccgctacc cggattcgcc
      421 ggtcagcctg caggtggcca attcggaaac cgttgccggc cgcctgctgc ggggcgactg
      481 cgatttcggc ctgatcgagg gcgaggtggc gcatcccgac ctgctggtcg agccctggct
      541 ggccgacgag ctggtcgtct tctgcgcgcc ggggcacgcg ctggccggcc gcacggcggt
      601 cgacagcgcg accctgggcg aacagtcctg ggtcctgcgc gaaccggggt cgggcacgcg
      661 cagccagttc gaccgctgca tcggcagccg cctgccggcg ttccggctgg cgctcgaact
      721 cgagcacacc gaggcgatca agcgcgccgt cgaatccggc atcggcctcg gctgcctgtc
      781 gcggctggcg ctgcgcgaag ccttccggcg cggcagcctg gtcgaactgc cgacgccgca
      841 gttcgacctg cgccggcact tctacttcgt ccgccagcgc agccgccacc ccggcctcgc
      901 cggccgcctg ttccgcgagg cctgcctggc ggtcggcggc ggcgccgccg atacggcggg
      961 cctgcaactg ccgttcattc cgtgagccgg cacgatgcca ggtggcgcgc cgtattccct
     1021 ggcgctggcg gcacggcgag gagctgttcg ttgcgcgcgg cgaattccct cgcgtcggcc
     1081 gaacggcgac cggatgagcg ggaatgccgg cggcgccgcg ccactgccgg ccttgcgcgt
     1141 tggtgtagag taggcatcgg ttgccggcgc cgaccggcgg cgaatcttcc aaccgcgcgc
     1201 ggtgcgtctt cgtcaggtgg tgcatgatgg aaatctggat tctggtcttc ctgattctgg
     1261 gctgtggcct gctcgccatg gcggaaatgg cgatcggcgc cagccgcaat acccatctgg
     1321 cgatccgcgc cgagcagggc aaggcggcag cggcggtggt catcggcttc cgccagaacg
     1381 ccagccgcct gcttggcacg acgcagctcg gcatcaccgc gttggcgatg ctctccggcg
     1441 tctatggcga ggcgctctgg gtgccgcgcg tcgaggcctg gctgctcggc ttcgggctga
     1501 cggcggggcc ggcctatgcg ctggcgctcg ccctggtggt gatcgtcatc accttcttct
     1561 cgctggtttt cggcgaggtg attccgaagc ggctggcgct ggcccacccg gaagccgtcg
     1621 ccgaggcgct gtccggcctg atcgccttcc tgatccgcct gacccggccg ctggtttcgg
     1681 tcgtttcgaa aaccgccgac tggctgctgc agttcttccc gctcaagggg tcggcggaag
     1741 cgacggcggc cgacgagatc cgcttcctga tcgaagccgg gcgcaagcac ggcgacctcg
     1801 accagaccga aagcgaaatc ctcggcaacg tcttccgcct cgacaaccgg aaggtggcag
     1861 cgatcatgac gcccgctgcc gacatcgcct gcctcgacct gagcgagtcg cacgaaacca
     1921 acatcgcgct gctgcgcgag cgtgccgtgt cgcgctacct ggtcagcaag ggcgggctcg
     1981 gcaacgcgct gggctttgtc gacagcggcg acctgttgcg cgaactgctt tccgagcgcg
     2041 aactcgactt cggcaagctg gcgctgcagc ccctgcagta cgtgccgggc agcctgtcgc
     2101 tgatcggcct gctcgaattc ttcaaggcca accagacgca gatcgcgctg gtctccaacg
     2161 agttcggcgc caccgaaggg ctggtcaccc tgtccgacct gatgggcacg gtcgtcggcg
     2221 acgtcctctc cggcgccgtc gagtcgccgc tggcgataca acgcaacgac ggtagctggc
     2281 tgctcgacgg tctgctgccg atcgacgaga tgaagcagtt gctcggcatc ggcgaactgc
     2341 cggaagagga actgggcaac taccacaccg tcggcggctt cgtgatcgtc cagctcgggc
     2401 ggattccccg caagaccgaa gccttcgact gggccggctg gcatttcgag gtcatggaca
     2461 tggaaaagaa ccgtgtcgac gaggtgctgg cgacgcgcct gagcgactga gtccggcagc
     2521 gtataagcaa cgcagttttt attatttttg gcgcgacagt cgggcgctta gagtgcgttc
     2581 acttatttca accaaggaag tgaagcatga agcactcagt catccgtcgc gcgctgctgg
     2641 ccagcgcgct gttcgccggg ctggccggcg ctggtctcgc ccaggcgcag accacgctgc
     2701 tcaacgtctc ctacgacccg acgcgcgaac tgtacgtcga gttcaacaat gccttcgcca
     2761 agcactggaa ggcgacgacc ggccaggacg tttcggtcaa gcagtcgcac ggcggctcgg
     2821 gcaagcaggg gcgggcggtg atcgacgggc tggaggccga cgtcgtcacc ctggcgctgg
     2881 cctacgacat cgacgcaatt tcccaggcat cgaagggcaa gctgctcccc gccaactggc
     2941 agcagcgcct gccgcacaac agctcgccct acacctcgac catcgtcttc ctggtcagga
     3001 agggcaaccc gaaggggatc aaggactggg acgacctggt caagccgggc gtcgaagtga
     3061 tcacgccgaa cccgaagact tccggcgggg cgcgctggaa ctacctggcc gcctggggct
     3121 acgcgctgaa gaagtacggt agcgaagcca aggcccaggc cttcgtcaag gacctgctgc
     3181 agcacgtgcc ggtgctcgat tccggtgccc gcggctcgac caacaccttc gtccagcgcg
     3241 gcatcggcga cgtgctgctg gcctgggaga acgaagcctt cctgtcgatc aacgaactcg
     3301 gcccggacaa gttcgagatc gtcgtgccat cgctctcgat ccttgccgag ccgccggtga
     3361 cggtggtcaa tggcgtgacc gagaagcgcg gcacgagcaa gctggcgacc gcctatctcg
     3421 agtacctgta ttcggccgag ggccagaaga tcgctgccaa gaactactac cggccgatca
     3481 agccggaact cgccgacaag aaggacctgg cgcgctttcc caagctcaac ctgatcaaga
     3541 tcgatgagct tggcggttgg caagccgccc agaagaagca ctttgccgac ggcggcacct
     3601 tcgaccaggt gtacggcaag aagtaaatcg ccggggtgcc accctcctga
//
LOCUS       DAOSDX010000191         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_8950_length_3647_cov_2.825445, whole genome shotgun sequence.
ACCESSION   DAOSDX010000191 DAOSDX010000000
VERSION     DAOSDX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8950_length_3647_cov_2.825445"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLN02_05395"
     CDS             complement(<1..583)
                     /locus_tag="PLN02_05395"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289316.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOI51791.1"
                     /translation="MSVSIPPKFNPLTGAIRALAMDAVQQANSGHPGAPMGMAEIAEV
                     LWRRHLRHNPANPAWPDRDRFVLSNGHGSMLIYALLHLTGYDVSIDDLKNFRQLHART
                     PGHPEYGYTPGVETTTGPLGQGITNAVGFALAEKVLAAEFNRPGHDIVNHHTYTFLGD
                     GCLMEGVSHEACSLAGTLGLGKLIAFWDDNGISI"
     gene            791..1393
                     /locus_tag="PLN02_05400"
     CDS             791..1393
                     /locus_tag="PLN02_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOI51792.1"
                     /translation="MQYLLVLSRLIDAFSERLGKLIYWLVLLAVLISAGNAVVRKTLN
                     IGSNAFLEIQWYLFSAVFLLGAGFVFMKNAHVRIDFLSNRFSPKVRNLIDITGILLIL
                     WPFCVVFIDLSWPLFTGAWASGEMSQNAGGLIRWPVYLMMPLGFGLLLLQSVSELIKR
                     FAFLAGMIPDPIGHNPHAPEEDVLSYEIEEHAGRAREEKR"
     gene            1393..>2288
                     /locus_tag="PLN02_05405"
     CDS             1393..>2288
                     /locus_tag="PLN02_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOI51793.1"
                     /translation="MAFIAEYFVPLMFVGLIFFLLSGFPVAFALAATGLLFGTVGMEA
                     GLFPATLFQALPLRLFGIMQNDTLLAIPFFTLMGIILQKSGMAEDLLESIGQVFGPLR
                     GGLALAVIIVGALLAATTGVVAASVIAMGLISLPIMLRYGYNRAIAAGTITASGTLAQ
                     AIPPSLVLIVMADQLGRSVGDMYAGAMIPAFMLVGLYVIFIVGLAIFKPDWVPALPPE
                     ARIYREPNGRSGQLSLWLLSAGSWAIGLLLFFNYDAVADSLFGRKGLAPFDERAIFTL
                     SVIGIVALLLAIINKQLKLGLLS"
     assembly_gap    2289..2298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2299..3133
                     /locus_tag="PLN02_05410"
     CDS             <2299..3133
                     /locus_tag="PLN02_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOI51794.1"
                     /translation="TFALIPPLVLIFLVLGTIFLGIATPTEGGAMGAVGALIMALSRR
                     QLSLTLLKEALDNSARLSCFVLFILIGSTVFSFTFNAADGHIWVEHLFDKLPGGQIGF
                     LVFVNLLVFVLGMFIDFFEIAFIVVPLLAPVAEKMDINLIWFGVLIAMNLQTSFLTPP
                     FGFALFYLRSVAARTAYKDAVTGKQIEPLTTPQIYKGAIAFIILQLIMVAVLIMYPGL
                     VTGSLDEKVKVDLNTIELDAGSSSWGDQPALPDPIAPPSDPAADPAAALMEEMRQDAK
                     R"
     gene            complement(3259..>3647)
                     /locus_tag="PLN02_05415"
     CDS             complement(3259..>3647)
                     /locus_tag="PLN02_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI51795.1"
                     /translation="AYPAWWEGGPQLTLYVSDKAYNSLSKEYKAIIECASAYAHVDMQ
                     AKYDARNPTALKELVAGGTKLFRLPKDVMDAAFKFSQEHYAELSAKNPNWKKIYADYE
                     VFRRDQHQWFRFSEAGFDSYMQSQKL"
BASE COUNT          544 a         1123 c         1160 g          810 t
ORIGIN      
        1 cgatcgagat gccgttgtcg tcccagaagg cgatcagctt gcccaggccg agcgtgccgg
       61 cgagcgagca ggcttcatgc gagacgccct ccatcaggca gccgtcgccg aggaaggtgt
      121 aggtgtggtg attgacgatg tcgtggccgg ggcggttgaa ctcggcagcc agcaccttct
      181 cggccagcgc gaagccgacg gcgttggtga tgccctggcc gagcggcccg gtggtcgtct
      241 cgacgcccgg cgtgtagccg tattccgggt ggcccggggt cctggcgtgg agctggcgga
      301 agttcttcag gtcgtcgatg ctgacgtcgt agccggtcag gtgcagcagc gcgtagatca
      361 gcatcgaacc gtggccgttt gacagcacga agcggtcgcg gtcgggccat gccggattgg
      421 ccgggttgtg gcgcaggtgg cgccgccaca ggacttcggc gatctcggcc atgcccatcg
      481 gcgcgcccgg gtgaccggag ttggcttgct ggacggcgtc catggctagc gcgcggatgg
      541 cgccggtcag ggggttgaac ttgggaggaa tgctcacgct catgatccgg gatccagcct
      601 gctgaaaagc cgtaattatc ggcgaaaagc cggcttttgg atagctggcc agcggtttgc
      661 cggtgcttgt gggggctgtt cgccaatcat cttccagcct ctcagggcta tccctaattt
      721 ttgtgcgact ggccggtggg tagactgcta cgcagagatg ccgcccttcg tgcggcagag
      781 gaggatgaca atgcagtatc tgctcgtgct gtcgcgtttg atcgatgcgt tcagcgaacg
      841 tctcgggaaa ttgatttact ggctggtcct gcttgccgtg ctgatttctg ccggcaatgc
      901 ggtcgtgcgc aagacgctca atatcggttc caatgccttc ctggaaatcc agtggtacct
      961 gttctcggcg gtcttcctgc tcggcgcggg cttcgtgttc atgaagaacg cccacgtccg
     1021 catcgatttc ctgtccaacc gtttctcgcc caaggtgcgc aatctcattg acatcaccgg
     1081 catcctgctc atcctctggc ctttctgcgt ggttttcatc gacctttcct ggccgctgtt
     1141 cactggcgcc tgggctagcg gggaaatgtc gcaaaacgcc ggggggctga tccgttggcc
     1201 ggtatacctg atgatgccgc tcggtttcgg tttgctgctg ctgcagtcgg tctccgaact
     1261 gatcaagcgt tttgccttcc tcgccggaat gatccccgat cctatcggcc acaacccgca
     1321 cgcccccgag gaggatgtcc tgtcctacga aatcgaagag cacgccggtc gtgcccgtga
     1381 ggagaagcgc tgatggcctt catcgccgaa tactttgtcc ccctgatgtt cgtgggcttg
     1441 attttcttcc tcctctccgg ttttccggtg gccttcgcgc tggccgccac cggcctgctg
     1501 ttcggtaccg tcggcatgga ggccgggctg ttcccggcca ccctgttcca ggctttgccg
     1561 ttacgcctgt tcggcatcat gcagaacgat accctgctgg ccattccctt cttcacgctg
     1621 atgggcatca tcctgcagaa gagcggcatg gccgaggatc tgctggagtc gatcggccag
     1681 gtcttcggcc cgttgcgcgg cgggctggcg ctggccgtga tcatcgtcgg cgccctgctg
     1741 gcggcgacca ccggggtggt tgccgcgtcg gtgattgcaa tggggctgat ctcgctgcca
     1801 atcatgctgc gctacggtta caaccgcgcc atcgccgccg ggacgatcac cgcctccggc
     1861 accttggcgc aggcaattcc gccctcgctg gtgctcatcg tgatggccga ccaactcggt
     1921 cgctcggtcg gcgacatgta tgccggggcg atgattccgg ccttcatgct ggttgggttg
     1981 tacgtgatct tcatcgtcgg tctggcgatt ttcaagccgg actgggtgcc ggcgctgccc
     2041 cccgaggcac ggatttaccg cgaaccgaac gggcgcagcg ggcagttatc gctgtggctg
     2101 ctgtcggccg gttcctgggc gatcggcctg ctgctcttct tcaattacga cgcggttgcc
     2161 gatagcctgt ttggcagaaa ggggctggcg ccgttcgacg agcgcgccat cttcaccctg
     2221 tcggtgatcg gtatcgtcgc cctgctgctg gcgatcatca acaagcaact gaagctcggc
     2281 ctgctttcnn nnnnnnnnca cctttgcatt gatcccgccg ctggtgctga tcttcctggt
     2341 gctcggcacg atcttcctcg gcatcgccac gccgaccgaa ggcggcgcga tgggggcggt
     2401 gggggcgctg atcatggcgc tgagccgccg tcagctgtcg ctgaccttgc tcaaggaggc
     2461 gctcgacaac agcgcccgcc tgtcctgttt cgtactcttc atcctgatcg gctcgacggt
     2521 gttttccttc accttcaacg ccgccgacgg gcacatctgg gtcgagcacc tgttcgacaa
     2581 gctgcccggc gggcagatcg gtttcctggt gttcgtgaac ctgctggtct tcgttctcgg
     2641 catgttcatc gacttcttcg agattgcctt catcgtcgtc ccgctgctgg cgccggtggc
     2701 ggagaagatg gacatcaacc tgatctggtt cggcgtgttg atcgcgatga acctgcagac
     2761 ctccttcctc accccacctt tcggtttcgc gctgttctac ctgcgcagcg tcgccgcccg
     2821 caccgcttac aaggatgcgg tgaccggcaa gcagatcgaa ccgctgacga cgccgcagat
     2881 ctacaagggg gcgattgcct tcatcatcct gcagctaatc atggtggcgg tgctgatcat
     2941 gtaccccggc ctggtgaccg gcagtctgga cgaaaaggtc aaggtcgatc tcaacaccat
     3001 cgagctcgac gccggcagca gttcctgggg cgatcagccc gccctgccgg acccgattgc
     3061 gccaccgagc gatcccgcgg ccgacccggc ggcagccctg atggaggaaa tgcgccagga
     3121 cgccaagcgc tgaggccttt tctcccggca acgaaaaccg cccggatgta atgaccgggc
     3181 ggtttttttg tgccctgggt tttccgccca caaaaaaccc gcagaccggc tgcgggttgt
     3241 acgggcgagg tgcctgcctc agagtttctg cgactgcatg tagctgtcga agccggcttc
     3301 cgagaagcgg aaccactggt gctggtcgcg gcggaagact tcgtagtcgg cgtagatctt
     3361 cttccagttc gggttcttcg ccgacagctc ggcgtagtgt tcctgcgaga acttgaaggc
     3421 ggcgtccatg acgtccttcg gcagacggaa cagcttggtg ccgccggcga ccagttcctt
     3481 cagcgcggtc gggttgcggg cgtcgtactt ggcctgcatg tcgacgtgtg cgtaggccga
     3541 ggcgcactcg atgatcgcct tgtattcctt ggacaggctg ttgtaggcct tgtcgctgac
     3601 gtacagcgtc agttgcgggc cgccttccca ccaggccggg taggcgt
//
LOCUS       DAOSDX010000192         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9034_length_3624_cov_3.770244, whole genome shotgun sequence.
ACCESSION   DAOSDX010000192 DAOSDX010000000
VERSION     DAOSDX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9034_length_3624_cov_3.770244"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PLN02_05420"
     CDS             <1..1246
                     /locus_tag="PLN02_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI51796.1"
                     /translation="QRMTLTTGGYSDYERARAARLAQQQSAYEAQQREIARLSRFVER
                     FKAKATKARQAQSRVKMLERMEIVAAAHADSPFQFSFRAPDSLPDPLLLIENAAAGYA
                     ERRILENLTLTLRPGCRIGLLGRNGAGKSTLIKLLAGSIAQQGGERKEAKALNIGYFA
                     QHQLEQLRPEETPLWHLQKLEPLTPEQELRDYLGGFDFRGEMASRPVGPFSGGEKSRL
                     ALALLIRTRPNLLLLDEPTNHLDLEMREALAFALQDYEGGMVVVSHDRHLLRSCTDEL
                     WLVDNGAATPFDGDLDDYAAWLAAQRNADKAPAADAQAEKAERLQQRADAKANRQALL
                     AERRKLTKEIEQIDRNLAKWQEEKAALDTQFADPDFYATVDRQKNEEMHRRAGELGDA
                     IDTAELRWLELHEALDALPAVD"
     gene            1380..1988
                     /gene="thrH"
                     /locus_tag="PLN02_05425"
     CDS             1380..1988
                     /gene="thrH"
                     /locus_tag="PLN02_05425"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOI51797.1"
                     /translation="MKIVCLDLEGVLVPEIWIEFAERTGIPELKRTTRDEPNYDTLMT
                     YRLNLLREKQLGLPDIQKVIAEMGPMQGARAFLDQLREDYQVVILSDTFYEFAHPLMR
                     QLGWPTLFCHSLEADADGMLVNYHLRMPNQKQEAVKRFKELNFTIVAAGDSYNDTAML
                     GEAHGGILFHPPENVVREFPQFPVVRDYDALRAEIDKAFAKA"
     gene            2089..2952
                     /gene="purU"
                     /locus_tag="PLN02_05430"
     CDS             2089..2952
                     /gene="purU"
                     /locus_tag="PLN02_05430"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOI51798.1"
                     /translation="MHRDRFYTLSASCPDQVGIIARVSGFIAEHGGWILESSFHADDL
                     TGRYFMRIEIKADSLPFLLAEFRERFRRDVAEPLAMDWRINDSAVKKRVVVLVSKQEH
                     CLYDLLARWQSKELDIEIPCVISNHDTFKGFVEWHGIPFIHVPVNADNKAAAYAEIQR
                     IFDDVSGDTMVLARYMQILSPELCDALSGKIINIHHSFLPSFVGARPYHQAYQRGVKL
                     IGATCHYVTSELDAGPIIEQDVIRIDHSDSPEDMVRYGKDIEKTVLARGLRYHLEDRV
                     LVHGNKTVVFR"
     gene            2952..3494
                     /locus_tag="PLN02_05435"
     CDS             2952..3494
                     /locus_tag="PLN02_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOI51799.1"
                     /translation="MLLSRADSLLLVVDIQERLAPAIHDSETVTANSVRLLEAAGQLE
                     VPAFVSEQYVKGLGPSLAAIREAASAAHFFEKIHFSCAVEPGVTDLLRASGRRQIVLT
                     GTETHVCVLQTALGMLGEGFSVFLVADAASSRTPENRQLAIDRLRSAGVHIVSTEMVL
                     FEWLQRAGTDEFRALLPLIR"
BASE COUNT          702 a         1269 c         1061 g          592 t
ORIGIN      
        1 gcagcggatg acgctgacca ccggcggcta ttccgattac gagcgcgccc gcgccgcccg
       61 cctggcccag cagcaatcgg cctacgaagc ccagcaacgg gaaatcgccc gtttgagccg
      121 cttcgttgaa cgcttcaagg ccaaggcgac caaggcgcgc caggcacaaa gccgggtcaa
      181 gatgctcgaa cgcatggaaa tcgtcgccgc cgcgcacgcc gattcgccgt tccagttcag
      241 cttccgcgcc ccggacagcc tgcccgaccc gttactgctg attgaaaatg ccgccgccgg
      301 ctacgccgag cgcaggattc tggaaaacct cacgctgacc ctgcgcccgg gctgccggat
      361 cggcctgctg gggcgcaacg gcgccggcaa atcgacgctg atcaaactgc tcgccggcag
      421 catcgcccag cagggtggcg agcgcaagga agccaaggcg ctgaacatcg gctacttcgc
      481 gcagcaccag ctcgaacaac tgcgccccga ggaaaccccg ctctggcacc tgcagaagct
      541 cgaaccgctg acgccggaac aggaactgcg cgactacctg ggcggcttcg atttccgcgg
      601 cgagatggcg agccgtccgg tcggcccctt ctccggcggc gaaaaatcgc gcctggcgct
      661 cgccctgctc atccgcaccc ggcccaacct gctgctgctc gacgaaccga ccaaccacct
      721 cgacctcgaa atgcgcgaag cgctcgcctt cgcgctgcag gactacgaag gcggcatggt
      781 cgtcgtctcg cacgaccgcc acctgctgcg cagttgcacc gacgaactgt ggctggtcga
      841 caacggcgcc gccacgcctt tcgacggcga ccttgacgac tacgccgcct ggctggccgc
      901 ccagcgcaac gccgacaagg cgccggccgc cgacgcccag gccgaaaaag ccgagcgcct
      961 gcagcaacgc gccgacgcca aggccaaccg gcaagcgctg ctcgccgaac ggcgcaagct
     1021 gaccaaggaa atcgagcaaa tcgaccgcaa cctggccaaa tggcaggaag aaaaggctgc
     1081 gctggacacc cagttcgccg atcccgactt ctacgccacg gtcgaccgcc agaaaaacga
     1141 agaaatgcac cgccgcgccg gcgaactcgg cgacgcgatc gacaccgccg agctgcgctg
     1201 gctggaactg cacgaggcac tggacgcact gccggcggtg gattgaaggc tgtcgccggg
     1261 ttaccggttt cccgtgaaac gtttgccctc ccgggacaag caattccgct ttcatttccg
     1321 ctgagacgcc gtccgttcgg gtaaaatcgc gcttttttgg ccgtctcagg gagttgaccg
     1381 tgaaaatcgt ctgtcttgac ctcgaagggg tcctggttcc ggaaatctgg atcgaattcg
     1441 ccgagcgtac cggcatcccc gaactgaagc ggacgacgcg cgacgagccg aactacgaca
     1501 cgctgatgac ctaccggctg aacctgctgc gcgagaagca gctcggcctg ccggacatcc
     1561 aaaaggtgat cgccgaaatg ggcccgatgc agggcgcccg cgctttcctc gaccagctgc
     1621 gcgaggacta ccaggtggtc atcctgtccg acaccttcta cgaatttgcc catccgctga
     1681 tgcgccaact cggctggccg acgctgttct gccattcgct ggaagccgac gccgacggca
     1741 tgctggtcaa ctaccacctg cgcatgccga accagaagca ggaagcggtc aagcgcttca
     1801 aggaactgaa cttcaccatc gtcgctgccg gcgattcgta caacgacacg gcgatgctcg
     1861 gcgaagccca cggcggcatc ctcttccacc cgccggaaaa cgtcgtccgc gaattcccgc
     1921 agttcccggt ggtgcgcgat tacgacgcgc tacgcgccga aatcgacaag gctttcgcca
     1981 aggcctgacg gccattggcg caagcctgcg gcgcactgca cccccccgct cactgcgttc
     2041 gccgcccccc cgggggggct ggacgcttcg acatagcctg tgaacaccat gcatagagat
     2101 cgtttttaca cgctgtccgc ctcctgcccc gatcaggtcg ggatcatcgc ccgcgtttcc
     2161 ggcttcatcg ccgagcatgg cggctggatt ctcgaatcga gtttccacgc ggacgacctg
     2221 accggtcgct atttcatgcg catcgagatc aaggccgatt cgctgccttt cctgctcgcc
     2281 gagttccgtg agcgcttccg ccgcgatgtc gccgaaccgc tggcgatgga ctggcggatc
     2341 aacgacagcg cggtgaagaa acgcgtcgtc gttctggttt ccaagcagga gcactgcctc
     2401 tacgacctgc tggcgcgctg gcagtcgaag gaactggaca tcgagattcc ctgcgtcatc
     2461 tccaaccacg acaccttcaa gggtttcgtc gagtggcacg gcattccctt catccacgtg
     2521 ccggtcaacg ccgacaacaa ggccgccgcc tacgccgaaa tccagcgtat cttcgacgat
     2581 gtgagcggcg acaccatggt gcttgcccgc tacatgcaga tcctctcgcc ggaactttgc
     2641 gacgccttgt ccggcaagat catcaacatc caccacagct tcctgcccag cttcgtcggc
     2701 gcccgcccct accatcaggc ctaccagcgc ggcgtcaagc tgatcggcgc gacctgccat
     2761 tacgtgacca gcgaactcga cgccggaccg atcatcgaac aggacgtgat ccgtatcgac
     2821 cattccgatt cgccggaaga catggtccgc tacggcaagg acatcgagaa gaccgtgctc
     2881 gcccgcggcc tgcgctatca cctggaagac cgcgtgctgg tacacggcaa caagacggtg
     2941 gttttccgct gatgctgctc tcccgcgccg attcgctgtt gctcgtcgtc gatatccagg
     3001 aaaggctggc accggcgatt cacgacagcg agacggtcac cgccaattcg gtacgcctgc
     3061 tcgaagccgc cggtcagcta gaggtaccgg ccttcgtttc cgaacagtac gtcaagggac
     3121 ttggccccag cctcgccgca atccgtgaag ccgccagcgc cgcgcacttt ttcgaaaaga
     3181 tccacttctc atgcgccgtc gaacccggcg tcaccgacct gctgcgcgcc agcgggcgcc
     3241 ggcagattgt cctgaccggc accgaaaccc acgtctgcgt gctgcagacg gcgctcggca
     3301 tgctcggcga gggtttttcg gtatttctcg tcgccgatgc cgcgtcgtcg cgcacaccag
     3361 agaaccgcca actggcgatc gaccgcctgc gcagcgccgg cgtacacatc gtcagtaccg
     3421 agatggtgct gttcgaatgg ctgcagcgcg ccgggacgga cgagttccga gccttgctgc
     3481 cgctgattcg ctgaacgcgt cagtagggaa tgactggcgc cgcctgcagc ggcagataat
     3541 ggctgcgcaa gggcaagccc ggcagcaagt catgcaccag cagccccggt ggctcgggtg
     3601 tccaggacaa cgccccgtcc tgca
//
LOCUS       DAOSDX010000193         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9037_length_3623_cov_3.084922, whole genome shotgun sequence.
ACCESSION   DAOSDX010000193 DAOSDX010000000
VERSION     DAOSDX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9037_length_3623_cov_3.084922"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PLN02_05440"
     CDS             <1..1601
                     /locus_tag="PLN02_05440"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOI51800.1"
                     /translation="LSTAPPSTHQSAGATGAAVAWRLGQAGLDVLCLEQGEHMDPAHY
                     PSTRPDWELARLQDFHVSPNVRRREADYPINDSDSPIAIANFNAVGGSTILFSGHFPR
                     FHPSDFRVRSLDGVADDWPINYPQLAPYFALNDRMMGVSGLGGDPAYPPIDGLLPPVP
                     MGRLGECLGRGFNRLGWHWWPAYAAIATRPHHGRPPCINLGPCNTGCAQGAKASTDVS
                     YWPAARQSGVRLQTGARVSRVTLDAQGKASGVLYFDAAGNEWQQPARLVVLACNGVGT
                     PRLLLNSACPAFPDGLANRSGLVGRNLMLHPLGYVEGLFDEALDSQLGPQGCSIYSHQ
                     FYETDSARGFVRGYTMQVLRGPGLLETATSGFLRREIPPGPGFAAALRERFGHLAAMG
                     IIVEDLPDPDNRVTLDGELTDSHGIPAPKIHYTLGDNSRRMLAHGLERGKEVMRAAGA
                     RRTAAFGPVRHTGWHLMGTARMGNDPARAVVDARGRCHDVDNLYIVDSSVFVTSGGVN
                     PASTMQAIALHIADGIVARRQSAA"
     gene            1598..2056
                     /locus_tag="PLN02_05445"
     CDS             1598..2056
                     /locus_tag="PLN02_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51801.1"
                     /translation="MSSAAETGDRTLAVLLDDLLPPADGVPTIDAPRLAEFAARHSRS
                     APLARLLDIVAAASPGTPFADLDAAARAALLSRIRRQHLAAFADFFLLAIQCYCLDPA
                     VRQAFGDDPAAPFPNGRSLPDGDLELLEAVYERGPLFRDPHALTDKEQTP"
     gene            2053..3171
                     /gene="gmd"
                     /locus_tag="PLN02_05450"
     CDS             2053..3171
                     /gene="gmd"
                     /locus_tag="PLN02_05450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI51802.1"
                     /translation="MKKALITGVTGQDGSYLAEFLLAKGYKVHGIKRRASSFNTQRID
                     HIYEDPHVENARFELHYGDLTDSSNLTRILAEVQPDEVYNLGAQSHVAVSFEAPEYTA
                     DVDAIGTLRLLEAIRFLGLEKKTRFYQASTSELFGLVQETPQRETTPFHPRSPYAVAK
                     MYAYWITVNYREAYGMYACNGILFNHESPRRGETFVTRKITRGLANIAQGLEKCLYMG
                     NLDALRDWGHAKDYVRMQWMMLQQAQPDDFVIATGSQHSVRQFIEWSAAELGITLAFE
                     GEGVEEKGRVVAIDGDKAPAVRPGDIVVAIDPRYFRPAEVSTLLGDPSKAKAKLGWEP
                     EITAREMCAEMVAHDLAQARQHALLKQHGYQASAWHFE"
     gene            3182..>3623
                     /locus_tag="PLN02_05455"
     CDS             3182..>3623
                     /locus_tag="PLN02_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOI51803.1"
                     /translation="MKILLTGASGMVGRNLLAHPAAARHAWLTPGSGELDLLDFAAVR
                     HYLQASQPDLVIHAAGRVGGIQANLREPVRFLVDNLDMGRNVLLAAAETGITRLLNFG
                     STCMYPRGRQQALREDDVLSGELEPTNEGYALAKITVARLAAYLA"
BASE COUNT          609 a         1379 c         1118 g          517 t
ORIGIN      
        1 gcctgtcgac agcgcccccg tcaacccatc aaagcgccgg cgccaccggt gccgcggtcg
       61 cctggcgcct cggccaggcc gggctggacg tgctgtgcct ggagcagggc gaacacatgg
      121 acccggcaca ttacccgagc acccgcccgg actgggagct ggcccgcctg caggacttcc
      181 acgtcagccc caacgtccgc cgccgggaag ccgactaccc gatcaacgac agcgattcgc
      241 caatcgccat cgccaatttc aatgccgtgg gcggcagcac gatcctcttt tccggccact
      301 tcccgcgctt tcacccatcg gatttccggg tccgcagcct cgacggcgtc gccgacgact
      361 ggccgatcaa ctacccgcaa ctcgccccct acttcgccct caacgatcgc atgatgggag
      421 tttccgggct cggcggcgat cccgcctacc cgccgatcga cggcctgctg ccgccggtgc
      481 cgatgggccg cctcggcgaa tgcctcgggc gcggcttcaa ccggctcggc tggcactggt
      541 ggccggccta cgcggcaatc gccacgcgtc cgcaccacgg ccgcccgccc tgcatcaatc
      601 tcggcccgtg caacaccggc tgcgcccagg gcgccaaggc cagcaccgac gtcagctact
      661 ggccggcagc caggcagtcc ggcgtacgcc tccagaccgg cgcccgggtc agccgcgtca
      721 ccctcgatgc gcaggggaaa gccagcggcg tcctctattt cgacgccgcc ggcaacgaat
      781 ggcagcagcc ggcacggctc gtcgtcctcg cctgcaacgg cgtcggcacc ccgcgcctgc
      841 tgctcaattc cgcctgcccg gcctttcccg acggcctggc caaccgctcg gggctggtcg
      901 gcagaaacct gatgctgcac ccgctgggtt acgtcgaagg cctgttcgac gaagcgctcg
      961 acagccagct cggcccgcag ggctgctcga tctacagcca ccagttctac gaaaccgata
     1021 gcgcccgcgg cttcgtgcgc ggctacacga tgcaggtgct gcgcggcccg gggctgctgg
     1081 agaccgccac gagcggcttc ctgcgccgcg aaatcccgcc cggccccggc ttcgctgcgg
     1141 cgctgcgcga acgcttcggc catctcgcgg ccatgggcat catcgtcgaa gacctgccgg
     1201 acccggacaa ccgcgtcacg ctggacggcg aactcaccga cagccacggc atcccggcgc
     1261 cgaaaatcca ttacacgctg ggcgacaaca gccggcgcat gctcgcccac gggctcgagc
     1321 gcggcaagga agtcatgcgc gccgccggcg cccggcgcac cgcggccttc ggcccggtac
     1381 gccacaccgg ctggcacctg atgggcaccg cgcgcatggg caacgacccg gcccgcgccg
     1441 tggtcgacgc ccggggccgt tgccacgatg tcgacaatct ctacatcgtc gacagcagcg
     1501 tcttcgtcac ctcgggcggc gttaacccgg cctcgacgat gcaggccatc gccctgcaca
     1561 tcgccgacgg catcgtcgcc agaaggcaga gtgccgcatg agttcggctg cggaaaccgg
     1621 cgaccggacg ctcgccgtcc tcctcgacga cctgctgcca ccggcggacg gcgtgccgac
     1681 gatcgacgcc ccccggcttg ccgagttcgc ggcgcggcac agccgcagcg cgccactcgc
     1741 caggctgctg gatatcgttg ccgcagcatc gcccgggacg cccttcgccg atctcgacgc
     1801 ggcggcgcgc gccgcgctgc tgagccgcat ccgccgccag catctggcgg ccttcgccga
     1861 cttcttcctg ctcgccatcc agtgctactg tctcgatccc gcggtccgcc aggccttcgg
     1921 cgacgacccg gccgccccct tcccgaacgg gcgcagcctc ccggacggcg atctcgaact
     1981 gctggaagcc gtctacgaac gcggccccct attccgcgat cctcacgctt tgaccgacaa
     2041 ggagcagact ccatgaaaaa agccctgatc accggcgtca ccggtcagga cggttcctac
     2101 ctggcggagt tcctgctcgc caagggctac aaggtgcatg gcatcaagcg ccgggcgtcg
     2161 tccttcaaca cccagcgcat cgatcacatc tacgaagatc cgcacgtcga gaacgcccgt
     2221 ttcgaactgc actacggcga cctcaccgat tcctcgaacc tgacgcgcat cctcgcggag
     2281 gttcagcccg acgaggtcta caacctcggc gcccagagcc acgtcgcggt cagcttcgaa
     2341 gcacccgaat acaccgccga cgtcgacgcg atcggcaccc tgcgcctcct ggaggcaatc
     2401 cgtttcctcg gcctggaaaa gaagacccgc ttctaccagg cgtccacttc cgaactcttc
     2461 ggtctggtgc aggaaacccc gcagcgcgaa acgacgccct tccacccgcg tagcccctac
     2521 gccgtggcga agatgtacgc ctactggatc accgtcaatt accgcgaggc ctacggcatg
     2581 tacgcctgca acggcatcct gttcaaccac gaatcgccgc gtcgcggcga aaccttcgtt
     2641 acccgcaaga tcacgcgcgg cctcgccaac atcgcccagg ggctggagaa atgcctgtac
     2701 atgggcaatc tcgacgcgct gcgcgactgg ggccacgcca aggactacgt gcgcatgcag
     2761 tggatgatgc tgcagcaggc gcaaccggac gatttcgtca tcgccaccgg cagccagcac
     2821 agcgtgcgcc agttcatcga atggagcgcc gccgaactgg gcatcaccct cgctttcgaa
     2881 ggcgaaggcg tcgaggagaa ggggcgggtg gtcgccatcg acggcgacaa ggcgccggcc
     2941 gtgcggccgg gcgacatcgt ggtcgccatc gacccgcgct atttccggcc ggccgaagtg
     3001 tcgacgctgc tcggcgaccc gtccaaggcc aaggccaagc tgggctggga accggagatc
     3061 acggcccggg aaatgtgcgc cgaaatggtc gcccacgacc tggcgcaggc ccgccagcac
     3121 gccttgctca agcaacatgg ctaccaggca tcggcctggc acttcgagta gggcgcggcg
     3181 catgaagatc ctgctgaccg gcgcctccgg catggtcggg cgcaacctcc tggcgcaccc
     3241 ggcggcggca cggcacgcat ggctgacgcc aggcagcggc gagctggacc tgctggattt
     3301 cgctgccgtc cggcactacc tgcaggccag ccagccggac ctggtgatcc acgccgccgg
     3361 ccgggtcggc ggcatccagg ccaacctgcg cgagccggtg cgcttcctcg tcgacaacct
     3421 cgacatgggg cgcaacgtgc tgctcgccgc cgccgaaacc ggcatcaccc ggctgctcaa
     3481 cttcggcagc acctgcatgt atccgcgcgg ccggcaacag gcgctgcgcg aggacgacgt
     3541 gctgagcggc gaactggaac cgaccaacga gggctatgcg ctggccaaga tcacggtggc
     3601 gcggctggcc gcctacctgg ccc
//
LOCUS       DAOSDX010000194         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9075_length_3612_cov_2.988755, whole genome shotgun sequence.
ACCESSION   DAOSDX010000194 DAOSDX010000000
VERSION     DAOSDX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9075_length_3612_cov_2.988755"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLN02_05460"
     CDS             <1..625
                     /locus_tag="PLN02_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOI51804.1"
                     /translation="FHAARFANAYAASKYAAEQAIRALAAHHPATRYTMLRPRALFGP
                     HDRVILPRLLRQIERDGGVLRLPRGGVAVLDLTYVGNVVHALQLATTQPQLPAAAVYN
                     IGNHQPGRLRDLLDRLLRQEMGLAYRLQALPYPLLHGVAAGMEAVAALTRREPPLTRY
                     SVGAMNFDMTLDNRRAIAELGYRPIIPLAEGIRRTAAWQRGEETVDG"
     gene            618..1424
                     /locus_tag="PLN02_05465"
     CDS             618..1424
                     /locus_tag="PLN02_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI51805.1"
                     /translation="MAELTAFTVGYCTHPAGMALRGAGWQVCRFPARAYLLAVGGRHW
                     LWDTGYAEHFRQHTSAGIFRLYAAITPVHFAAHEALLGQLRGVGLTPRDICGLLLSHF
                     HGDHIAGLHDFAATPCICSGEGWQRTRGLRGFGALRRAFVPGLIPAGFETTLQFVESF
                     PLLRLPAELAPFEWAWALPESRNEILLVPLPGHAAGQIGAFVHTADGWTLLASDAAWA
                     PQGYREQRGPARLTHLLMDDRAAYYDTPARLQALHRGGKVRILLCHEGEL"
     gene            1876..3228
                     /locus_tag="PLN02_05470"
     CDS             1876..3228
                     /locus_tag="PLN02_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HOI51806.1"
                     /translation="MPYTGQRSSSENRAGGRASLETRRMIALTTLWHYLRTRRLQFAD
                     RAALLAHQDRQLRRYARRVLTRSPYFARCAELHFAAWPTMDKALMMQHFDAINTAGLK
                     RDALLAVARRSEAERDFTPRVGRYSVGLSSGTSGQRGLFVVSPREQAAWAGVMLARLL
                     PAGLTAGERVALFLRADNHLYHSVDNHWLSLSFHDLFAPFEAQCARLQAERPSIVVAP
                     AQVLRALALAVRDGSLRIAPKLVISVAEVLEPADRALLGEVFPRVGEVYQATEGFLAA
                     TCPQGTLHLNEEFLRIEPQWLDEQRFTPWVTDFTRQTQPIVRYRLDDVLVRRAEPCPC
                     GRAGLALAAIEGRCDDQLLLPGADGQVKPVFADLCSRLIANALPLTADYRLEQHGADR
                     LQLTADCPLADIERCRAALIAGFARQGVAVAALHWRLASQPLPADFRHKRRRIRRLAS
                     "
     gene            3225..>3612
                     /locus_tag="PLN02_05475"
     CDS             3225..>3612
                     /locus_tag="PLN02_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51807.1"
                     /translation="MTSTAFRLRQMLFGWGGVGLVYNLSDRFQGPGLVLEPGFIDRLI
                     PYSPHAVWPYLSFFLIVPLAFLTCPAGRLRWLRSAFQVAALAAGTVFMLWPTTLAYPD
                     YSVSGLSGRLLAGLLAVDSPQNCFPSL"
BASE COUNT          479 a         1450 c         1140 g          543 t
ORIGIN      
        1 attccacgcc gcgcgcttcg ccaatgccta cgccgccagc aagtacgccg ccgagcaggc
       61 gatccgcgca ctcgccgccc accatcccgc cacccgctac acgatgctgc gcccgcgcgc
      121 actgttcggc ccgcacgacc gcgtcatcct gccgcgcctg ctgcgccaga tcgaacgcga
      181 cggcggcgtc ctgcgcctgc cgcgcggcgg cgtcgccgtg ctcgacctca cttatgtcgg
      241 caacgtcgtg cacgcgctgc agctcgccac cacgcagccg caactgccgg ccgccgccgt
      301 ctacaacatc ggcaaccacc agccgggccg cctgcgggac ctgctcgacc gcctgctgcg
      361 ccaggaaatg ggcctcgcct accgcctgca ggcgctgccc tacccgctgc tgcacggcgt
      421 cgccgccggc atggaggcgg tcgccgccct cacccggcgc gaaccgccgc tgacccgcta
      481 cagcgtcggc gcgatgaact tcgacatgac cctcgacaat cgccgcgcca tcgccgaact
      541 cggctaccgc ccgatcatcc cgctggccga gggcatccgc cgcaccgccg cctggcagcg
      601 cggcgaggaa accgtcgatg gctgagctga ccgccttcac cgtcggctac tgcacacacc
      661 cggccggcat ggcgctgcgc ggcgccggct ggcaggtctg ccgctttccc gcgcgcgcct
      721 acctgctcgc cgtcggcggc cgccactggc tgtgggatac cggctacgcc gagcatttcc
      781 gccagcacac cagcgctggc atcttccgcc tctacgcggc gatcacgccg gtgcatttcg
      841 ccgcccacga ggcgctgctc gggcaactgc ggggcgtcgg cctgacgccg cgcgacatct
      901 gcgggctgct cctctcgcac ttccacggcg accacatcgc cggcctccac gacttcgccg
      961 ccacgccctg catctgctcg ggcgaaggct ggcagcgcac gcgcggcctg cgcggtttcg
     1021 gcgcgctgcg ccgcgctttc gtgccggggc tgatcccggc cggtttcgag acgacgctgc
     1081 aattcgtcga aagttttccg ctgctgcgcc tgccggccga actggcaccc ttcgaatggg
     1141 cctgggcgct gccggagagc cgcaacgaaa tcctgctcgt cccgctgccc ggccacgccg
     1201 ccggccagat cggcgccttc gtgcacaccg ccgacggctg gaccctgctc gccagcgacg
     1261 ctgcctgggc gccgcagggc taccgcgagc agcgcggccc tgcccgcctc acccacctgc
     1321 tgatggacga ccgcgccgcc tactacgaca ccccggcccg cctgcaggcg ctgcatcgcg
     1381 gcggcaaggt gcgcatcctg ctgtgccacg agggggaact gtgaacgacc ggcccgccct
     1441 gccattgcgc cactccggcc gagcgcccac tggctccgac tgccgacttc agcgccccac
     1501 ccttgccagc atgcgcccat gggaaccagc caagcggcgt acttccgtgg aaagccgtcc
     1561 cggcccggca cgcgtctgca gcaacccgcc gcgcgatcac gctgaccgcg ccatggcatt
     1621 acccgggcgc acgacgcctg caattcgctc accgcaccgc gctgcccgcc cagcagggcc
     1681 ggcagttgcg gaattacccg cggtttcagc gccctacacc ggccaacgta gctccgtgga
     1741 aagccgcgcc ggtaaacacg cgtcgttgga ataccgccgc atgatcgcgc cgagcacgcg
     1801 ctggcaccac ctgcgcacgc ggcgcctgct gctcgcccac ctaggccggc agttgcggca
     1861 ttacccgcgg cttcaatgcc ctacaccggc caacgcagct cctcggaaaa ccgcgccggc
     1921 gggcgcgcgt cgttggaaac ccgccgcatg atcgcgctga ccacgctctg gcactacctg
     1981 cgcacgcggc gtctgcagtt tgccgaccgc gccgcgttgc tcgcccatca ggaccggcag
     2041 ttgcggcgtt acgcgcggcg cgtgctgacg cgcagcccct atttcgcgcg ctgcgccgaa
     2101 ctgcatttcg ccgcctggcc gacgatggac aaggcgctga tgatgcagca cttcgacgcc
     2161 atcaacaccg ccggcctcaa gcgcgacgcg ctgctggcgg tggcgcggcg cagcgaggcc
     2221 gagcgcgact tcacgccgcg cgtcggccgc tacagcgtcg ggctgtcgtc cggcacctcg
     2281 gggcagcgcg ggctgttcgt cgtcagcccg cgggagcagg cggcctgggc cggcgtcatg
     2341 ctggcgcgcc tgttgcccgc cggactgacc gctggcgagc gcgtcgccct cttcctgcgc
     2401 gccgacaacc acctctacca cagcgtcgac aaccactggc tgagcctttc cttccacgac
     2461 ctgttcgcgc ccttcgaagc gcagtgcgcg cgcctgcagg cggagcggcc gagcatcgtc
     2521 gtcgccccgg cgcaggtgct gcgcgcgctg gcgctggcgg tgcgcgacgg cagcctgcgc
     2581 atcgcgccca agctggtgat ttcggttgcc gaagtgctgg agccggccga ccgggcgctg
     2641 ctcggcgagg tatttccgcg cgtcggcgag gtttaccagg cgaccgaggg tttcctcgcc
     2701 gccacctgcc cgcagggcac gttgcacctg aacgaggaat tcctgcgcat cgagccgcag
     2761 tggctggacg aacagcgctt cacgccctgg gtcaccgact tcacgcggca gacccagccg
     2821 atcgtccgct accgcctcga cgacgtcctg gtaaggcgcg ccgagccctg cccctgcggc
     2881 cgcgccggcc tggcgctggc ggcgatcgag gggcgttgcg acgaccagtt gctgctgccc
     2941 ggcgccgacg ggcaagtcaa accggtgttc gccgacttgt gcagccgcct gatcgccaac
     3001 gcgctgccgc tgaccgccga ctaccggctc gaacagcacg gtgccgaccg cctgcaactg
     3061 accgccgact gcccgctggc cgacatcgag cgttgccgcg cggcgctgat cgccggtttc
     3121 gcccgccagg gcgtcgccgt cgccgccttg cactggcggc tcgccagcca gccgctgccc
     3181 gccgatttcc ggcacaagcg gcggcgcatc cggcgcctcg cctcatgacc agcaccgcat
     3241 tccggctgcg ccagatgctc ttcggctggg gcggcgtcgg ccttgtctac aacctcagcg
     3301 accgcttcca ggggccgggg ctggtcctcg aacccggctt catcgaccgc ctgattccct
     3361 acagcccgca cgcggtgtgg ccctacctgt cgttcttcct gatcgtcccg ctcgccttcc
     3421 tgacctgccc ggccggacgc ctgcgctggt tgcgcagcgc tttccaggtg gcggcgctgg
     3481 ccgccggcac ggtcttcatg ctgtggccga ccaccctcgc ctatcccgat tattccgtca
     3541 gcggactcag cggcaggctg cttgccggcc tgctcgccgt cgattcgccg cagaactgct
     3601 ttccctcgct gc
//
LOCUS       DAOSDX010000195         3606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9092_length_3606_cov_3.192340, whole genome shotgun sequence.
ACCESSION   DAOSDX010000195 DAOSDX010000000
VERSION     DAOSDX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9092_length_3606_cov_3.192340"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLN02_05480"
     CDS             <1..456
                     /locus_tag="PLN02_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HOI51808.1"
                     /translation="VTLAADSRYLGWDILCLGRAAAGERFDHGRFDLHCRIERAGKPL
                     WIERGSVAGGDAMLHSPAGWAGATVCGTLLCSFPELPQQATALLEALRALTPGDGAQH
                     GITAPPGLLIARYLGDNSEAARLWFAELWKILRPACCGRPAVLPRIWNT"
     gene            467..769
                     /gene="ureA"
                     /locus_tag="PLN02_05485"
     CDS             467..769
                     /gene="ureA"
                     /locus_tag="PLN02_05485"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HOI51809.1"
                     /translation="MELTPREKDKLLIFTAGLLAERRKAKGLKLNYPEAVALITCAIM
                     EGAREGKTVAELMHAGTQVLTRADVMDGIAELIPEIQVEATFPDGTKLVTVHNPIV"
     gene            780..1085
                     /locus_tag="PLN02_05490"
     CDS             780..1085
                     /locus_tag="PLN02_05490"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HOI51810.1"
                     /translation="MIPGELLAEPGELELNAGRPTITLVVANTGDRPIQVGSHYHFHE
                     TNAGLSFDREAARGYRLDIAAGTAVRFEPGQTRTVQLVALAGERKVYGFRGLVQGAL"
     gene            1085..2791
                     /gene="ureC"
                     /locus_tag="PLN02_05495"
     CDS             1085..2791
                     /gene="ureC"
                     /locus_tag="PLN02_05495"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HOI51811.1"
                     /translation="MANKITRQAYAEMFGPTTGDRLRLADTELIIEVEKDYTIYGEEV
                     KFGGGKVIRDGMGQSQRVSAETVDTVITNALIVDAVTGIVKADIGLKDGRIAAIGKAG
                     NPDIQPGVTIVVGPGTEVIAGEGMIVTAGGIDSHIHFICPQQIDEALMSGVTTMLGGG
                     TGPATGTYATTCTPGPWHIHRMLEAAEAFPMNLGFLGKGNASLPEALREQVAAGVIGL
                     KLHEDWGTTPAAIDCCLSVADEMDVQVAIHTDTLNESGFVEATIAAFKGRTIHTFHTE
                     GAGGGHAPDIIKATSLPNVLPSSTNPTMPYTVNTIDEHLDMLMVCHHLDSSIAEDVAF
                     AESRIRRETIAAEDILHDMGVFSMMSSDSQAMGRVGEVIIRTWQAAHKMKVQRGTLPE
                     DSARHDNFRVKRYIAKYTINPALTHGIAHTVGSIEVGKLADLVLWKPAFFGVKPSLIL
                     KGGMIAAAAMGDPNASIPTPQPVHYRPMFGSFGKALKTSVTFVSQAALENPAVAALGL
                     SKPLVAVSGCRGVQKSDMVHNGATPEITVDPETYVVKADGVHLVCEPATELPLAQRYF
                     LF"
     gene            2794..3333
                     /gene="ureE"
                     /locus_tag="PLN02_05500"
     CDS             2794..3333
                     /gene="ureE"
                     /locus_tag="PLN02_05500"
                     /inference="COORDINATES: protein motif:HMM:NF009751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HOI51812.1"
                     /translation="MLILNKRTTAPATDSVALAYDERKRSRLKVTLASGSEAGIFLER
                     GDHLHGGDKLAAENGSAVVEILAAPEKLIEAVADSPLLFARAAYHLGNRHVPVQILPT
                     ENGGKLRFQTDHVLADMARGLGCSISETEAPFQPDSGAYGAHAGHHHHGEEPEHADLH
                     DPGHGPHRSVPKIHQFRNR"
     gene            complement(3356..>3606)
                     /locus_tag="PLN02_05505"
     CDS             complement(3356..>3606)
                     /locus_tag="PLN02_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOI51813.1"
                     /translation="LLATYRQRGLAFHALRTRQSGARAFITLHVLVPGQWTVQQGHDL
                     VEEIEAGIRAAVPRSHVLTHLEPIEDAGSLADLELDRS"
BASE COUNT          684 a         1281 c         1101 g          540 t
ORIGIN      
        1 gtcacgctcg ccgccgacag ccgctatcta ggctgggaca tcctctgcct gggccgggcg
       61 gcggccggcg aacgcttcga tcacgggcgt ttcgacctgc actgccgcat cgagcgcgcc
      121 ggcaaaccgc tgtggatcga gcgcggcagc gtcgccggcg gcgacgccat gctgcacagc
      181 cccgccggct gggccggcgc gacggtatgc ggcacgctgc tgtgcagttt tccggaactg
      241 ccgcaacagg ccaccgccct gctcgaagcg ctgcgcgcat tgacgcccgg cgatggcgcg
      301 caacatggca tcaccgcgcc gcccggtttg ctgatcgccc gttaccttgg cgacaacagc
      361 gaagccgccc ggctgtggtt cgccgaactg tggaaaatcc tgcgtccggc ctgctgcggc
      421 cgaccggccg tgctgccccg aatctggaat acctgaagga gtcgaaatgg aactgacacc
      481 gcgcgagaag gacaagctgc tgatcttcac cgccggcctc ttggccgagc ggcgcaaggc
      541 caagggcctg aagctcaact acccggaagc cgtcgcgctg atcacctgcg cgatcatgga
      601 aggcgcccgc gaaggaaaaa cggtggccga gctgatgcac gccggcaccc aggtgctgac
      661 ccgcgccgac gtgatggacg gcatcgccga gctgatcccg gaaatccagg tcgaagccac
      721 cttcccggac ggcaccaagc tggtcacggt ccataacccg attgtttgag gagaacacga
      781 tgatccccgg agaactcctc gccgaacccg gcgaactcga actcaacgcc ggccgcccga
      841 cgatcacgct ggtcgtcgcc aataccggcg accgcccgat ccaggtcggc tcgcactacc
      901 actttcatga aaccaacgcc ggcctgagct tcgaccgcga agccgcgcgc ggctaccgcc
      961 tcgacatcgc cgccggtacc gccgtgcgtt tcgaaccggg ccagacgcgc accgtgcaac
     1021 tggtcgcgct ggccggtgag cgcaaggttt atggtttccg cggcctcgtt cagggagctt
     1081 tgtaatggct aataaaatca cccgtcaggc ctatgccgaa atgttcggtc ccacgaccgg
     1141 cgaccgcctg cgtctggccg ataccgagtt gatcatcgag gtcgagaagg actacacgat
     1201 ctacggcgag gaagtgaaat tcggcggcgg caaggtcatc cgcgacggca tgggccagag
     1261 ccagcgcgtt tccgctgaaa cggtcgatac cgtgatcacc aatgcgctga tcgtcgatgc
     1321 ggtcaccggc atcgtcaagg ccgacatcgg cttgaaggac ggccgcatcg ccgccatcgg
     1381 caaggccggc aatccggaca tccagcccgg cgtgacgatt gtcgtcggcc ccggcaccga
     1441 ggtcatcgcc ggcgaaggca tgatcgtcac ggcgggcggc atcgacagcc acatccattt
     1501 catctgcccg cagcagatcg acgaagcgct gatgtccggc gtcaccacca tgctcggcgg
     1561 cggcaccggc ccggccaccg gcacctacgc cacgacctgc acacccggcc cctggcacat
     1621 ccaccgcatg ctcgaagccg ccgaagcctt cccgatgaac ctcggctttc tcggcaaggg
     1681 caacgccagc ctgccggaag cgctgcgcga gcaggtcgca gccggtgtca tcggcctcaa
     1741 gctgcacgag gactggggca cgacgccggc cgccatcgac tgctgcctgt cggtcgccga
     1801 cgaaatggac gtgcaggtcg ccatccatac cgacacgctc aatgaatcgg gcttcgtcga
     1861 agcgacgata gccgccttca agggccgcac catccacacc ttccacaccg aaggcgccgg
     1921 cggcggccac gcgccggaca tcatcaaggc gaccagcctg cccaatgtcc tgcccagctc
     1981 gaccaacccg accatgccgt acacggtgaa caccatcgac gagcacctcg acatgctgat
     2041 ggtctgccac cacctcgatt cgagcatcgc cgaggacgtc gcctttgccg aatcgcgcat
     2101 ccgccgcgag accatcgctg ccgaagacat cctgcacgac atgggcgtct tctcgatgat
     2161 gtcgtccgac tcgcaggcca tgggccgcgt cggcgaagtg atcatccgca cctggcaggc
     2221 ggcgcacaag atgaaggtcc agcgcggcac cctgccggaa gatagcgccc gccacgacaa
     2281 cttccgcgtc aagcgctaca tcgccaaata cacgatcaac ccggcgctga cgcatggcat
     2341 cgcgcacacc gtcggctcga tcgaagtcgg caagctggcc gacctggtgc tgtggaagcc
     2401 cgccttcttc ggcgtcaaac cgagcctgat cctcaagggc ggcatgatcg ccgccgccgc
     2461 gatgggcgac cccaacgcct cgatcccgac gccgcaaccg gtgcattacc ggccgatgtt
     2521 cggcagcttc ggcaaggcgc tgaagacttc ggtcaccttc gtttcgcagg cggcgctgga
     2581 gaatcctgcc gtcgccgcgc tcggactgtc gaagccgttg gtcgccgtct ccggttgccg
     2641 tggcgtgcaa aaatccgaca tggtgcacaa cggcgccacc ccggaaatca ccgtcgatcc
     2701 cgaaacctac gtggtcaagg ccgacggcgt gcacctggtc tgcgaaccgg cgaccgagct
     2761 gccgctagcc caacgctatt tcctgttctg accatgctga tcctgaataa acgtaccacc
     2821 gcccccgcca ccgactcggt ggcgctcgcc tacgacgaaa gaaagcgtag ccgcctcaag
     2881 gtgacgctgg cttccggcag cgaggccggc atttttcttg agcgcggcga ccacctgcat
     2941 ggcggcgaca agctggcggc cgaaaacggc agcgcggtcg tcgaaatcct cgccgcgccg
     3001 gaaaaactga tcgaggccgt cgccgacagc ccgctgctgt tcgcccgcgc cgcctaccac
     3061 ctcggcaacc gccatgtgcc ggtgcagatc ctgccgaccg aaaacggtgg caagctgcgt
     3121 ttccagaccg accacgtgct ggccgacatg gcgcgcggcc tgggttgcag catcagcgaa
     3181 acggaagcgc cgttccagcc ggattcaggt gcctatggcg cgcatgctgg acatcaccac
     3241 cacggtgaag aacccgagca cgccgatctg cacgatcccg gccacggccc gcaccgttcc
     3301 gtgccgaaaa tccaccagtt ccgtaaccgc tgaagcctgc acccgccaac aaccactagc
     3361 tgcggtccag ttcgaggtcg gccagcgagc cggcgtcctc gatcggttcg agatgggtca
     3421 gcacgtgcga acgcggcacg gcggcgcgga tgccggcctc gatttcctcg accaggtcgt
     3481 gcccctgctg caccgtccat tgtccgggca ccaggacatg cagggtgatg aaggcacgcg
     3541 cccccgactg gcgggtacgc agcgcgtgaa aggccagccc gcgctggcga taggtggcga
     3601 gtagcg
//
LOCUS       DAOSDX010000196         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9121_length_3595_cov_3.240113, whole genome shotgun sequence.
ACCESSION   DAOSDX010000196 DAOSDX010000000
VERSION     DAOSDX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9121_length_3595_cov_3.240113"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..647)
                     /locus_tag="PLN02_05510"
     CDS             complement(162..647)
                     /locus_tag="PLN02_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOI51814.1"
                     /translation="MAKPPSVRFLFAHPAHFFACGCGSGLAPWAPGTFGTLFAWASFI
                     LFRPHFGDGQLLFLFAVAYLAGIWFIEVAGRALGEPDHGSIVWDEIVPFWFVLLLAPP
                     GWLWQLAAFLLFRLFDISKPQPARWFDENVKNGFGVMADDLVAAGYTLFVLALLRHAL
                     S"
     gene            complement(640..1620)
                     /gene="thiL"
                     /locus_tag="PLN02_05515"
     CDS             complement(640..1620)
                     /gene="thiL"
                     /locus_tag="PLN02_05515"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOI51815.1"
                     /translation="MAGEFALIDRYFARPTPSAVLGPGDDCALVQPTPGKQLAVTTDM
                     LVAGTHFLADTDPRNLGWKALAVNLSDLAAMGATARWVTLAGALPAADAAWIERFADG
                     FFACAKEYGVDVIGGDTTRGPLNLCVTALGEVEPGRALRRDGARPGDQVWVSGRPGLA
                     ALGLAQLQGRVSLPEPWPRLCIAALERPRPRLALGQALVGLASAAIDVSDGLLADLGH
                     IAERSGCAAAVRLVQLPQLRDLVGSGERYDADLRRLALDCQLAGGDDYELCFTVPAAR
                     SPQVAALAAALELPLWPIGEMVAGTPGTVEVFDPDGRPVDIARRGYDHFG"
     gene            complement(1625..2095)
                     /gene="nusB"
                     /locus_tag="PLN02_05520"
     CDS             complement(1625..2095)
                     /gene="nusB"
                     /locus_tag="PLN02_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOI51816.1"
                     /translation="MTEQEQTPKAPPKSARRRAREFVLQGLYQWRVGGADEAAIEAYA
                     PEMEGFAKADREFFVGTLRGVIGQHEALIARIATHIDRPFGELSPVEASLLMLGTFEM
                     VNHPETPYRVIINEAIELAKSFGGTDGHKYVNGVLDKIAAIVRPDEVASRRRGK"
     gene            complement(2088..2564)
                     /gene="ribH"
                     /locus_tag="PLN02_05525"
     CDS             complement(2088..2564)
                     /gene="ribH"
                     /locus_tag="PLN02_05525"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOI51817.1"
                     /translation="MSRFDNVFEFDSNLDGNGLSIGIVMSRFNLPICEGLLSACVAEL
                     KRLGVADEDMAIATVPGALEIPLVLQTMAQTAQYDALIALGAVIRGDTYHFEVVSNDA
                     CRAIMELQLHTGVPIANGILTCDTDEQAEVRMLPKGTDCAQAAVEMANLQGALSND"
     gene            complement(2568..>3595)
                     /gene="ribBA"
                     /locus_tag="PLN02_05530"
     CDS             complement(2568..>3595)
                     /gene="ribBA"
                     /locus_tag="PLN02_05530"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOI51818.1"
                     /translation="NEGDLAMAAERVTPEAINFMAKYGRGLVCLTLTEARCRHLGLSQ
                     MARSNGTQFGTAFTVSIEAAEGVTTGISAADRAHTIRTAVARNASPADIVQPGHVFPI
                     TARDGGVLVRAGHTEAGCDLAGMAGLEPAAVICEIMNDDGTMARLPELIEFSKQHGLK
                     IGTIADLIHYRAASETLVERVSSKTIATAHGEFALHAYVDRAGGGTHLVLVKGEIPAG
                     GETLVRVHEPLSVLDFLDPASSRQSYSIDQAQAALARNGHGVIVLMHRPEDGAALLER
                     LTRPENTGAPRPQQKWDPRTYGIGAQILRDLGVGKMRLLSSPRKMPSMTGFGLEVTGF
                     VTSPAEL"
BASE COUNT          582 a         1148 c         1325 g          540 t
ORIGIN      
        1 gaagttcgag cagacctacc gctcgaacct ctacaacgac cgcgtcctga agaccctgca
       61 gatgatccgc cagaacagcc agtggctgat caccggcgaa accgccgtgc cctgccgcgg
      121 caacacggtc ggcggctgca acccggggca gcgctgacgg ttcaggaaag ggcgtggcgc
      181 agcagcgcca gcacgaacag cgtgtagccg gcggccacca ggtcgtcggc catcacgccg
      241 aagccgttct tgacgttttc gtcgaaccag cgcgccggtt gcggcttgct gatgtcgaac
      301 aggcggaaca gcaggaaggc cgccagttgc cagagccagc ccggcggggc gagcagcagc
      361 acgaaccaga aggggacgat ctcgtcccag acgatgctgc cgtggtcggg ttcgccgagc
      421 gcccggccag cgacctcgat gaaccagatg ccggccaggt aggcgacggc gaacaggaaa
      481 agcaactggc cgtcgccgaa gtggggacgg aacaggatga agctggccca ggcgaacagc
      541 gtgccgaagg tgcccggcgc ccacggcgcc agcccgctgc cgcagccgca ggcgaagaaa
      601 tgcgccgggt gggcgaacag gaagcggacg ctgggcggct tagccaaagt ggtcgtaccc
      661 ccggcgcgcg atgtccaccg gccgcccgtc cggatcgaag acctcgaccg tgcccggcgt
      721 cccggcaacc atctcgccga tcggccagag cggcagttcg agcgccgcgg cgagcgccgc
      781 cacttgcggg ctgcgcgccg ccggcacggt gaagcacagt tcgtagtcgt cgccgcctgc
      841 cagctggcag tcgagagcga ggcggcgcag gtcggcgtcg taacgctcgc cgctgccgac
      901 caggtcgcgc agttgcggca gttgcacgag gcggacggca gcagcacaac cggaacgttc
      961 ggcaatgtgg ccgaggtcgg cgagcaggcc gtcggaaacg tcgatcgccg cgctcgccag
     1021 gccgaccagc gcctggccga gggcaaggcg cgggcgcggc ctttccagcg cggcgatgca
     1081 caagcgcggc cagggttcgg gcagactgac ccggccctgc aactgggcga gaccgagcgc
     1141 cgccagcccg ggccggccgg acacccagac ctggtcgccg gggcgggcgc cgtcgcggcg
     1201 cagggcgcgc cccggttcaa cttcgccgag cgcggtgacg cagaggttga gcgggccgcg
     1261 cgtggtatcg ccgccgatca cgtcgacgcc gtactccttg gcgcaggcga agaaaccgtc
     1321 ggcaaagcgc tcgatccacg ccgcgtcggc cgccggcaac gcgccggcca gcgtcaccca
     1381 gcgcgccgtg gcgcccatcg ccgcgagatc ggagagattg acggcgagcg ccttccagcc
     1441 gaggttgcgg ggatcggtat cggccaggaa atgggtgccg gcgaccagca tgtcggtggt
     1501 caccgccagt tgcttgccgg gcgtcggctg gaccagcgcg cagtcgtcgc cggggcctag
     1561 caccgccgac ggcgtcggac gggcgaagta gcggtcgatc agcgcgaact cgcccgccat
     1621 cgccttactt gccgcgccgg cgcgaagcga cctcgtccgg acggacgatg gcagcgatct
     1681 tgtcgagcac gccgttgacg tacttgtggc cgtcggtgcc gccgaaggat ttggccagct
     1741 cgatcgcttc gttgatgatg acgcggtagg gcgtctccgg atgattgacc atctcgaagg
     1801 tgccgagcat cagcagcgag gcctcgaccg gcgacagctc gccgaagggg cggtcgatgt
     1861 gggtggcgat gcgggcaatc agcgcttcat gctggccgat gacgccgcgc agcgtgccga
     1921 cgaagaattc gcggtcggcc ttggcgaaac cttccatttc cggcgcatag gcttcgatgg
     1981 cggcctcgtc ggcaccgccg acgcgccact ggtagaggcc ctgcagaacg aattcgcggg
     2041 cgcgacgacg ggccgacttg ggcggcgcct tgggggtctg ttcctgctca gtcattgctc
     2101 agcgctcctt gcagattggc catttcgacg gcggcctggg cgcaatcggt gcccttcggc
     2161 aacatgcgga cttcggcctg ttcgtcggtg tcgcaggtca ggatgccgtt ggcgatcggc
     2221 acgccggtgt gcagttgcag ttccatgatg gcgcggcagg cgtcgttgga aaccacttcg
     2281 aagtgatagg tgtcgccgcg gatcaccgcg ccgagcgcga tcagcgcgtc gtactgggcg
     2341 gtctgcgcca tcgtctgcag gaccagcggg atttccaggg cgccggggac ggtggcgatc
     2401 gccatgtcct cgtcggcgac gccgagacgc ttgagttcgg cgacacaggc ggagagcagg
     2461 ccttcgcaga tgggcaggtt gaaacggctc atgacgatgc cgatggacag gccattgccg
     2521 tcgaggttgc tgtcgaattc gaatacgttg tcaaaacggg acatggctca aagctccgcg
     2581 ggtgaagtga cgaaaccggt gacttcgagg ccgaagccgg tcatggaggg catcttgcgc
     2641 gggctggaga gcaggcgcat cttgccgacg ccaaggtcgc gcaggatctg cgcaccgatg
     2701 ccgtaggtgc gcgggtccca tttctgctgc ggccgcgggg cgccggtgtt ttccgggcgg
     2761 gtcaggcgct cgagcagcgc cgcgccgtct tccgggcggt gcatgaggac gatgacgccg
     2821 tggccgttcc tggccagcgc cgcctgcgcc tgatcgatcg agtaggactg gcggctgctc
     2881 gcgggatcaa gaaaatcaag gaccgacagc ggttcgtgga cgcgcaccag ggtctcgcca
     2941 ccggcgggaa tctcgccctt gaccagaacc aggtgggtac cgccgccggc gcggtcgacg
     3001 taggcgtgca gcgcgaattc gccatgcgcg gtggcgatgg tcttgctgct gacgcgctcg
     3061 accagggttt cggaagcggc gcggtaatgg atgaggtcgg cgatggtgcc gatcttcagg
     3121 ccgtgctgct tggagaactc gatcagttcg ggcaggcggg ccatcgtgcc gtcgtcgttc
     3181 atgatctcgc agatcaccgc cgctggttcc aggccggcca tgccggcgag gtcgcaaccg
     3241 gcttcggtgt ggccggcgcg gaccaggacg ccgccgtcgc gggcagtgat cgggaagacg
     3301 tggccgggct ggacgatgtc ggccggactg gcgttgcgtg ccacggcggt gcggatggtg
     3361 tgcgcccggt cggcggccga gatgccggtg gtgacgcctt cggcggcttc gatcgagacg
     3421 gtgaaggcgg tgccgaactg ggtgccgttg ctacgcgcca tctggctcag gccgaggtgg
     3481 cggcagcggg cttcggtcag cgtcaggcag accaggccgc ggccatactt ggccatgaag
     3541 ttgatcgctt ccggcgtgac gcgctcggcg gccatggcca ggtcgccttc gtttt
//
LOCUS       DAOSDX010000197         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9181_length_3577_cov_3.985804, whole genome shotgun sequence.
ACCESSION   DAOSDX010000197 DAOSDX010000000
VERSION     DAOSDX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9181_length_3577_cov_3.985804"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLN02_05535"
     CDS             <1..798
                     /locus_tag="PLN02_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOI51819.1"
                     /translation="CANPTVRKLSFTGSTATGIKLMQQCAPTLKKLSLELGGNAPFIV
                     FDDADVDAAVLGAMQSKYRNSGQTCVCANRFLVQDGIYERFTEKLAAAVGQLVLGVGT
                     DAGVTQGPLIDGNALAKVETLLADATAKGARVLCGGQRSALGGTFFEPTIVADLTPEM
                     RVAREEIFGPVAPIFRFSSEEEAIQLANATEFGLAAYFYSKDLGRVWRVSEALEYGMV
                     GVNTGAISTEVAPFGGIKSSGFGREGSKYGIEDYLEIKYVCMAGIGR"
     gene            826..2133
                     /gene="gabT"
                     /locus_tag="PLN02_05540"
     CDS             826..2133
                     /gene="gabT"
                     /locus_tag="PLN02_05540"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HOI51820.1"
                     /translation="MNAHHEITRASNAALMQRRAAALPRGVGQGHPVFAERAENAEIW
                     DVEGRRWIDFCAGIAVVNTGHCHPRVIAAAHAQLDKFTHTCFQVVAYESYVELAEKLN
                     RLAPGTTPKKTFFMNTGAEAVENAIKVARAYTGRSGIIAFQGAFHGRTMYTMALTGKV
                     DPYKVGFGPFPGEIFHAPYPNALHGVSVDDSIAGLEAIFKNDIEARRVAAIFAEPVQG
                     EGGYLPAPPEFFVRLRELCDRHGILLVADEIQTGAARCGTMFAIEHSGIEPDIITMAK
                     GLGGGLTLSAVIGKAEIMDAATPGGLGSTYAGNPIAVAGALAVLDVIEEEQLCARAER
                     IGQAMRKKLNALAGRLDCIAEVRGLGAMTAVEFCRAGDPHRPAPEIANALKAEAAKRG
                     LLLLNCGTYGNVLRLMVPLTIPDAVLAEGMEILVAAIEVAAQA"
     gene            complement(2170..3024)
                     /locus_tag="PLN02_05545"
     CDS             complement(2170..3024)
                     /locus_tag="PLN02_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOI51821.1"
                     /translation="MEAGFVVALALLAFVVVTIAKGVRLVPQGEEWIVERLGKYHGTL
                     KPGLNVVIPYVDRIAYQLVTKDIILDVQEQEVITRDNAVILTNAIAFVKVTDPVKAVY
                     GVTDFSEAIRNLIMTTLRSIVGEMELDEALSSRDKIKARLRESIADEAIDWGLTVKSV
                     EIQDIKPSQSMQKAMEMQAAAERERKAVVTRAEGAKQSAILEAEARLESAKRDANAQV
                     MLAEASAEAIRRVSAAIGDQSGPMSYLLGEKYITAMERLGEKDNAKLVVLPADLQEAV
                     RGLFGRKG"
     gene            complement(3035..3478)
                     /locus_tag="PLN02_05550"
     CDS             complement(3035..3478)
                     /locus_tag="PLN02_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOI51822.1"
                     /translation="MQPEWWHWIVGGIALVLAELAMPAFYIIWFGLGALLVGLLLLLA
                     PGLSLTAQIVSWIVASLAMTWLWFRVFRKAQQTTLSGTADGEAIGEIGLLVGAIAPFE
                     KGRVRFQRPLLGADEWVCRADSAIAAGERVRVVAVEGSYLKVVKN"
BASE COUNT          607 a         1180 c         1165 g          625 t
ORIGIN      
        1 tgcgccaacc cgacggtgcg caagctgtcc ttcaccggct cgacggcgac cggcatcaag
       61 ctgatgcagc aatgcgcgcc gacgctcaag aagctgtcgc tcgagctagg cggcaacgcg
      121 ccgttcattg tcttcgacga cgccgatgtc gacgccgcag tgctcggcgc gatgcagtcg
      181 aagtaccgca acagcgggca gacctgcgtt tgcgccaacc gcttcctggt ccaggacggc
      241 atctacgagc gcttcacgga aaagctcgcg gcggcggtcg ggcaactggt gctcggcgtc
      301 ggtaccgacg ccggcgtcac ccaggggccg ttgatcgacg gcaacgcgct cgccaaggtc
      361 gaaaccctgc tcgccgatgc caccgccaag ggcgcgcgcg tgctctgcgg cggtcagcgc
      421 agcgcgctgg gcggcacctt cttcgaaccg accatcgtcg ccgacctgac cccggaaatg
      481 cgcgtggcgc gcgaggaaat cttcgggccg gtggcgccga tcttccgttt cagcagcgag
      541 gaagaggcga tccagcttgc caacgcgacc gagttcgggc tcgccgctta tttctacagc
      601 aaggacctgg gccgcgtctg gcgcgtttcg gaagcgctgg aatacggcat ggtcggcgtc
      661 aataccggcg ccatttcgac cgaagtggcg ccattcggtg gcatcaagtc ctccggcttc
      721 ggccgcgagg gatcgaagta cggaatcgag gattacctcg aaatcaaata tgtctgcatg
      781 gccggcatcg gccgctgacg aaacggatca tcggagaaca gcaccatgaa tgcacatcat
      841 gaaatcaccc gcgcaagcaa cgccgccctc atgcagcggc gcgccgccgc cctgccgcgc
      901 ggtgtcggcc agggccaccc ggtatttgcc gagcgcgccg agaacgcgga aatctgggac
      961 gtcgaaggcc gccgctggat cgacttctgc gccggcatcg ccgtggtcaa caccggccat
     1021 tgccacccgc gcgtgatcgc cgccgcgcat gcccagctcg acaagttcac ccacacttgc
     1081 ttccaggtcg tcgcctacga gtcctacgtc gaattggccg agaagctgaa ccgcctggcg
     1141 ccgggtacga cgccgaagaa gaccttcttc atgaacaccg gcgccgaagc ggtcgaaaac
     1201 gcgatcaagg tggcgcgtgc ctacaccggg cgcagcggca tcattgcctt ccagggcgct
     1261 ttccacggcc gcaccatgta caccatggcg ctgaccggca aggtcgatcc gtacaaggtc
     1321 ggcttcggtc ccttccccgg cgaaatcttc cacgcgccgt accccaacgc gctgcacggc
     1381 gtttcggtcg acgattcgat cgccggtctg gaagcgatct tcaagaacga catcgaagcc
     1441 cgtcgcgtgg cggcgatctt cgccgagccg gtgcagggcg agggcggtta tctgccggcg
     1501 ccgccggaat tcttcgtccg cctgcgcgaa ctgtgcgacc gccacggcat cctgctggtt
     1561 gccgacgaga tccagaccgg cgccgcgcgc tgcggcacga tgttcgccat cgaacacagc
     1621 ggcatcgagc ccgacatcat caccatggcc aagggcctgg gcggtggtct gacgctgtcg
     1681 gcggtgatcg gcaaggccga gatcatggac gcggcaacgc cgggcggtct cggttcgacc
     1741 tacgccggca acccgattgc cgtcgccggt gcgctggccg ttctcgacgt gatcgaggaa
     1801 gaacagctgt gcgcccgtgc cgagcggatc ggccaggcga tgcgcaaaaa gctgaacgcg
     1861 ctggccggcc ggctcgattg catcgccgag gtgcgtggcc tgggcgcgat gaccgcggtc
     1921 gaattctgcc gggccggcga tccccatcgt ccggcgccgg aaatcgccaa cgcgctgaag
     1981 gcggaagcgg ccaagcgtgg cctgctgctg ctcaactgcg gtacctacgg caacgtgctg
     2041 cgcctgatgg tgccgctgac cattcccgat gccgtccttg cggaaggcat ggagattctg
     2101 gttgcggcca tcgaggttgc cgctcaggcc tgatcccgcg ctgtgccggg aggccctcgc
     2161 ggcctcccgt cagcccttgc ggccgaacag gccgcgcacc gcttcctgca gatcagccgg
     2221 caggacgacc aacttggcgt tgtccttctc gcccaggcgt tccatcgcgg tgatgtattt
     2281 ttcgccgagc aggtagctca tcggcccgga ctgatcgccg atcgccgcgc tgacgcgacg
     2341 gatcgcctcg gccgaggctt cggccagcat cacctgggcg ttggcgtcgc gcttggccga
     2401 ttcgaggcgt gcttcggctt ccagaatggc cgactgcttg gccccttcgg cgcgggtgac
     2461 cacggccttt ctttcccgct cggcggcggc ctgcatttcc atcgccttct gcatcgactg
     2521 cgacggcttg atgtcctgga tttcgaccga cttcaccgtc aggccccagt cgatcgcctc
     2581 gtcggcgatg ctttcgcgca gccgcgcctt gatcttgtcg cgcgacgaca gcgcttcgtc
     2641 gagttccatt tcgccgacga tcgagcgcag cgtggtcatg atcaggttgc ggatcgcttc
     2701 ggaaaagtcg gtgacgccat agactgcctt gaccgggtcg gtcactttga cgaaggcgat
     2761 ggcgttggtc aggatcaccg cgttgtcgcg cgtgatcact tcctgttcct ggacgtcgag
     2821 gatgatgtcc ttggtgacca gctggtaggc gatgcggtcg acgtagggga tgacgacatt
     2881 gagccccggc ttgagcgtgc cgtgatactt gcccagtcgt tcgacgatcc attcctcgcc
     2941 ttgcggcacc aggcggacgc ccttggcgat ggtgacgacg acgaaggcga gcagggcaag
     3001 ggcgacgacg aatccggctt ccatggcgaa actcctagtt tttgacgact ttcagataac
     3061 tgccttcgac ggcgacgacg cgtacccgct cgccggcggc gatggcgcta tcggcgcggc
     3121 agacccactc gtcggcgccg agcagcggcc gctggaaacg cacgcggcct ttttcgaacg
     3181 gtgcaatggc gccgaccagc agaccgatct cgccgatcgc ctcaccgtcg gcggtaccgc
     3241 tcagcgtcgt ctgttgcgcc ttgcggaaga cgcggaacca gagccaggtc atggccagcg
     3301 aggcgacgat ccaactgacg atctgcgcgg tcagcgacag gccgggggcg agtagcagga
     3361 gcagcccgac gagcagcgcg ccgaggccga accagatgat gtagaaggcc ggcatggcga
     3421 gttcggcgag caccagggca atgccgccga cgatccagtg ccaccattcg ggttgcatga
     3481 cgatatccat gccgccgagt atttcgggtt tgtccgccgt ggtcaaggcg ggctgtggtt
     3541 tttacggatt cagccgggcc gtgtcgcggc ttagaat
//
LOCUS       DAOSDX010000198         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9199_length_3569_cov_3.085088, whole genome shotgun sequence.
ACCESSION   DAOSDX010000198 DAOSDX010000000
VERSION     DAOSDX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9199_length_3569_cov_3.085088"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLN02_05555"
     CDS             complement(<1..912)
                     /locus_tag="PLN02_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51823.1"
                     /translation="MNQQGFRITLGLLCFVVQGSAMAIGLGELHGQPALGSRLELSIS
                     ILGQEKEGLDTSCFRLVRPSGGDDLPWLKQAELRLVPGRPAQLKISSQQVLRDPVMRL
                     AVHLACGHEVNREYTILASPSIDAAQENVVTPAVAAVVKEKRPAPARRAAKPSTSMPG
                     RQVASGAASSSAIASPKEVAQPGGDQLLVSAADGDGDLPLRVSPLLREQSAEASETQR
                     EMLRLEFRMLQALHEQAGGQLEMAEKLRAMEGALAELQGRALSLTESASENKASAPAQ
                     PAGAAASEEAVEEDSAWWFYGLLLGVVL"
     gene            complement(954..1619)
                     /locus_tag="PLN02_05560"
     CDS             complement(954..1619)
                     /locus_tag="PLN02_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOI51824.1"
                     /translation="MNLTDDLLGEMWYWAAWAAWVPLFALSVWRAPWKRLATSEQSNL
                     WLGMIVLLMLVWSMKAGVKPGLALHLLGANVFALAFGPHLAFIGLSLVTLGVTLNGGA
                     GILAYAVNALLLAGFSVLVSNQLFLRLSRVLPRHFFVYIFVNAFFCAGIVILSVGFVS
                     CLLLGVAGAYSWAYLFDEHFPYYLLLAFSESWLSGMVVTLFVVYRPQWVASFDDSSYL
                     SDK"
     gene            complement(1629..2561)
                     /locus_tag="PLN02_05565"
     CDS             complement(1629..2561)
                     /locus_tag="PLN02_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOI51825.1"
                     /translation="MTILVIAEHDQQTIKSATLNTVAAAVKIGGEVHLLVAGSACSGA
                     AAQAATLQGVAKVLVADAAQYETQTAENLAALVVANASAYTHILAPATTFGKNLLPRV
                     AALLDVAQISEITAVESADTFVRPIYAGNAMATVQSQDPVKVITVRTTAFDPVATGAA
                     APVESLAAAADTGLTVLQGRELTKSERPELGAAKIIVSGGRGLGSGENYHRLLEPLAD
                     KLGAALGASRAAVDAGFVPNDYQVGQTGKVVAPQLYVAVGISGAIQHLAGMKDSKVIV
                     AINKDPDAPIFQVADYGLVADLFETVPQLENSLG"
     gene            complement(2563..>2824)
                     /locus_tag="PLN02_05570"
     CDS             complement(2563..>2824)
                     /locus_tag="PLN02_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOI51826.1"
                     /translation="ETLQLSLPAVVTTDLRLNEPRYATLPNIMKAKKKPLDTVKPDVL
                     GVDVAPRLKTLKVVEPAKRSAGVRVADVAELVNKLKNEAKVI"
     assembly_gap    2825..2834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2835..3266)
                     /locus_tag="PLN02_05575"
     CDS             complement(<2835..3266)
                     /locus_tag="PLN02_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOI51827.1"
                     /translation="MKILVPVKRVVDYNVKVRVKADGSGVDLANVKMSMNPFDEIAVE
                     EAVRLKEAGIATEVVAVSCGPAACQETLRTAMAIGADRGILVETDVELQPLAVAKLLK
                     ALCDREQPQLVVCGKQAIDDDANQTGQMLAALSGWPQATFAS"
     gene            3382..>3569
                     /locus_tag="PLN02_05580"
     CDS             3382..>3569
                     /locus_tag="PLN02_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI51828.1"
                     /translation="MENQAVKPPIKSQGLRASRKAVSGRTQLDPERWIDVATDVLAKE
                     GIAGLRVEVLAKRCGVTKG"
BASE COUNT          778 a         1156 c          986 g          639 t
ORIGIN      
        1 cagcacaaca ccgagcagca agccatagaa ccaccaagcc gagtcctctt caaccgcttc
       61 ctcgctggcc gctgcaccag ccggctgggc tggcgccgag gccttgttct cggaagcact
      121 ctcggtcaac gacagcgccc gcccctggag ttcggcaagc gccccctcca tggcgcgcaa
      181 tttttccgcc atttcgagtt gcccacccgc ctgttcatgc aaggcctgca gcatacgaaa
      241 ctcgaggcgc agcatttcac gctgggtttc actggcctcc gccgactgct cgcgcaacag
      301 cggcgaaacg cgtagaggaa gatcgccatc accatcggcc gccgagacca aaagctggtc
      361 gccgcccggt tgcgcaactt ctttggggct agcgatagcg gatgacgaag ccgcgccact
      421 cgccacctga cgcccgggca tgctcgtcga cggcttggcc gctcgacgcg ccggcgccgg
      481 ccgcttttcc ttgacaacgg ccgcaacagc aggagtgact acgttctcct gagcagcatc
      541 gatgctgggc gaagccagaa tggtgtattc ccgattgact tcgtggccac atgccagatg
      601 gacggcaagc cgcattaccg gatcgcgcag cacctgttgc gaagaaatct tcagttgcgc
      661 cggcctcccc ggcacaagac gcaactccgc ctgcttcaac caaggcaggt catcgccacc
      721 gctcggccgc accagacgga aacaactggt atcgaggcct tccttctcct gccccaggat
      781 gctgattgat aattccaggc ggctgcccag cgcgggctga ccatgcagtt cacccagccc
      841 aatcgccatc gcgctgcctt gtacgacaaa gcacagcaag cctagcgtta tccgaaatcc
      901 ctgctgattc aacaatgccc ccagatgacg atcaaataag cggattgtat caactatttg
      961 tcggaaagat agctcgaatc atcaaaagac gccacccatt gcggccgata aacgacgaac
     1021 aacgtgacca ccattcctga caaccaggat tccgaaaatg ccaggaggag ataatacgga
     1081 aaatgctcat cgaaaagata ggcccatgaa taagccccgg ccaccccaag cagcaggcac
     1141 gacacgaacc ccacgctcaa gatgacgatc ccggcacaga aaaaagcatt tacgaaaata
     1201 tagacaaaga aatgcctggg caagacgcgg gacaagcgta gaaataactg gttgctgacc
     1261 agcacgctga atccggccaa cagcaaggca ttgaccgcgt aggccaggat cccggcacca
     1321 ccgttcaggg taacgcccaa agtcaccagg gagagcccga tgaatgccag atgcggcccg
     1381 aatgccaagg caaaaacatt tgcgccgagc aaatgcagcg ccagtcctgg cttgacccca
     1441 gccttcatac tccagaccag catcagcaag acaatcattc caagccacag attggactgc
     1501 tcggaagtcg ccagacgctt ccacggggcg cgccagacac tgagggcgaa aagcggcacc
     1561 caggcagccc atgccgccca gtaccacatt tcgcccaaga ggtcgtcggt gagattcacc
     1621 gacgcatttt agccgagact gttctcgagt tgcggaaccg tttcgaacag atcggccacc
     1681 agaccgtaat ccgccacctg gaagatcggc gcgtccggat ccttgttgat cgcgacgatc
     1741 accttggaat ccttcatgcc ggccagatgc tggatggcac cggatatacc gacagcaaca
     1801 tagagctgcg gcgcaaccac cttgccggtc tgaccaacct gatagtcgtt cggaacaaac
     1861 cccgcatcga ccgcagcccg gctggcgccg agcgcggcac cgagcttgtc ggcaagcggc
     1921 tcaagcaggc ggtgatagtt ttcgccgcta cccagaccgc gaccaccgga aacgatgatc
     1981 ttcgccgccc ccagttcagg acgctcggac ttggtcaact cgcgcccctg cagtaccgtc
     2041 aggcccgtgt ccgccgcagc cgccagactt tccacgggag cagcagcacc ggtagcgacc
     2101 gggtcgaaag ccgtcgtacg aacggtgatc accttgaccg ggtcctggct ttgcaccgtc
     2161 gccatcgcat tgccggcata gatcggacgg acgaaggtat cggcggactc caccgcggtg
     2221 atttcagaga tttgcgccac atcgagcaag gcggcaaccc gcggcagcag gttcttgccg
     2281 aaggtggtcg ccggcgccag gatgtgggta taagcggaag cattcgccac caccagcgcc
     2341 gccagatttt ccgccgtctg ggtttcatac tgcgcagcat cggccaccag caccttggcc
     2401 accccctgca gcgttgcagc ttgtgctgca gcgcccgaac aggcactacc ggcaaccagc
     2461 agatgaacct cgccaccgat cttgacggcg gcggccacgg tgttcagggt ggcggacttg
     2521 atcgtttgct gatcgtgttc ggcaataaca agaatcgtca tctcagatca cctttgcttc
     2581 gttcttgagt ttattgacca gttcggcgac atcggcaacc ctgacaccgg ccgagcgctt
     2641 cgccggctcg acgaccttca gggttttcag gcgcggcgcc acgtccacgc caaggacgtc
     2701 cggcttgacg gtatcgagcg gcttcttctt cgccttcatg atattcggca gggtcgcgta
     2761 gcgcggctcg ttcagacgca gatcggtggt caccaccgcc ggcagcgaca actgcagggt
     2821 ttccnnnnnn nnnnggaagc gaaggttgcc tgcggccaac cgctcaacgc agccagcatc
     2881 tgcccggtct ggttggcatc gtcgtcgatc gcctgcttgc cgcagacgac cagttgcggc
     2941 tgctcgcgat cgcagagtgc tttcagcaac ttggcgacgg ccagcggctg cagttcgaca
     3001 tcggtctcga ccaggatgcc gcggtcggca ccgatcgcca tcgccgtgcg caaggtttcc
     3061 tggcaggcgg ccggaccgca ggaaacggca acgacttcgg tcgcaatgcc ggcttccttg
     3121 agccgcaccg cctcttcgac ggcgatttcg tcgaaggggt tcatgctcat cttgacgttg
     3181 gccagatcga cgccgctgcc gtcggccttg acccgcacct tgacgttgta atccaccacc
     3241 cgtttgacgg gaacaagaat tttcactgtt ttctcctaat tatttcggtc agtattttta
     3301 ttgatcttcg ccgtttgaca gacgacttgg caaaccggac aatccccata ctgtgtcgta
     3361 tggaaaagca tacggtagcg tatggaaaat caagctgtca agcccccgat caagagccaa
     3421 ggtcttcgtg ccagtcgcaa ggcggttagc ggccgcaccc aactcgatcc ggaacgctgg
     3481 atcgatgtcg ccaccgacgt cctcgccaag gagggcatcg ccggtttgcg ggtggaggtc
     3541 ctggcaaaac gctgcggtgt caccaaggg
//
LOCUS       DAOSDX010000199         3551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9269_length_3551_cov_3.016876, whole genome shotgun sequence.
ACCESSION   DAOSDX010000199 DAOSDX010000000
VERSION     DAOSDX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9269_length_3551_cov_3.016876"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /gene="rfaD"
                     /locus_tag="PLN02_05585"
     CDS             <1..880
                     /gene="rfaD"
                     /locus_tag="PLN02_05585"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOI51829.1"
                     /translation="KNLIDCEIADYLDKHEFIEMVQAGHFDGAVEAIFHEGACSDTME
                     TDGHYMMENNYRYTSTLLDFCLDHETQFLYASSASVYGAGRVFKEARAHEGPLNVYGY
                     SKFLFDQVVRHRLAQQPSSQIVDFRYFNVYGPRESHKGRMASVAFHNFNQFRADGKVK
                     LFEGSHGYPDGGQMRDFVFVGDVAKVNLFFLDHPEKSGIFNLGSGRAQSFNDVAVAAA
                     NGCRKARGEAALGLDDLRAQGLLEYVAFPEALKGKYQAFTQADLTKLREAGYDAPMAT
                     VEEGVSQYIDWLHAHV"
     gene            873..1772
                     /gene="cysM"
                     /locus_tag="PLN02_05590"
     CDS             873..1772
                     /gene="cysM"
                     /locus_tag="PLN02_05590"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HOI51830.1"
                     /translation="MYKTLQDFVGNTPLVRLQRIPGVDNERRGNVILAKLEGNNPAGS
                     VKDRPALSMIQRAEARGDIKPGDTLIEATSGNTGIALAMAAAIKGYKMILVMPENQSI
                     ERRQSMRAYGAELVLTPKDGGMELCRDVAERMRDEGKGIILDQFANPDNPVAHYEGTG
                     PEIWRDTGGRISHFVSSMGTTGTIMGTSRYLKEMNPAITIVGCQPDEGSQIPGIRKWP
                     EAYLPKIYAPTNVDRIEYVSQADAEAMTRRLAVEEGIFAGISSGGALAVALRIAAQVE
                     NATIVSIVCDRGDRYLSTGVFPA"
     gene            1769..2554
                     /locus_tag="PLN02_05595"
     CDS             1769..2554
                     /locus_tag="PLN02_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOI51831.1"
                     /translation="MKPVLVFDIETIPDVAGLRRINELPGELSDAEVAELAFQQRRAK
                     NGSDFLPLHLQRIVAISCVLRTLDGFRVWSLAEPELDEGAIIQRFFDGIEKFTPQIVS
                     WNGGGFDLPVLHYRGMLHGVTAPRYWDMGDGDYADSRDFKWNNYISRYHTRHLDLMDL
                     LAMYNGRANAPLDELAKLFGFPGKLGMDGSKVWEAWQAGQAADIRDYCETDVVNTYLV
                     YNRFRRMRGEFTADEEMAEQAFIKGQLAKIDAPHWREFLAAWQ"
     gene            2618..3166
                     /gene="ppa"
                     /locus_tag="PLN02_05600"
     CDS             2618..3166
                     /gene="ppa"
                     /locus_tag="PLN02_05600"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOI51832.1"
                     /translation="MALNNVPSGKSLPDDFNVIIEITAHSDPVKYEVDKESGAIFVDR
                     FMSTAMHYPCNYGYIPHTIAGDGDPVDVLVISPFPLPPGVVVRCRPLGQLSMTDEGGE
                     DAKLLAVPVSKLTPLYNDVKTYEDTPELLRQQIAHFFEHYKDLEPGKWVKVNGWQGID
                     AAREEVMTGVQRYNEAKDKPNY"
     gene            3168..>3551
                     /locus_tag="PLN02_05605"
     CDS             3168..>3551
                     /locus_tag="PLN02_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOI51833.1"
                     /translation="MLRIAVAQFNAVVGDLTGNVDRIVACAVAAKARGAQVLLTPELA
                     LSGYPPEDLLLRPDFYRACQRALDDLAARIDGIALVVGHPEEHEGACYNAATVIENGR
                     PLAVYRKMRLPNYDVFDEKRYFDAGR"
BASE COUNT          697 a         1184 c         1067 g          603 t
ORIGIN      
        1 caagaacctg atcgattgcg agatcgccga ctatctcgac aagcacgagt tcatcgagat
       61 ggtgcaggcc ggccatttcg acggcgcggt ggaggcgatc ttccatgaag gggcctgttc
      121 ggacaccatg gagaccgacg gccactacat gatggagaac aactaccgct acaccagcac
      181 gctgctggat ttctgcctcg accacgagac gcagttcctc tacgcgtcga gcgcctcggt
      241 gtacggcgcc ggccgcgtct tcaaggaagc gcgcgcgcac gaggggccgc tcaacgtcta
      301 tggctactcg aagttcctgt tcgaccaggt ggtccgccac cgtctggcac agcagccgtc
      361 gtcgcagatc gtcgacttcc gctacttcaa tgtctacggc ccgcgtgaaa gccacaaggg
      421 gcgcatggcc tcggtcgcct tccacaactt caaccagttc cgcgctgacg gcaaggtcaa
      481 gctgttcgag ggctcgcatg gctacccgga cggcggccag atgcgcgatt tcgtcttcgt
      541 cggcgacgtc gccaaggtca atctgttctt ccttgaccat ccggaaaagt ccggcatctt
      601 caacctcggt tcggggcggg cgcagagctt caacgacgtt gcggtggctg cggccaacgg
      661 ttgccgcaag gcccggggcg aagccgcgct cggtctggac gatctgcggg cacagggctt
      721 gctcgaatac gtcgccttcc ccgaagcgct caagggcaag taccaggcgt tcacgcaggc
      781 cgatctgacg aaactgcgcg aagccggcta cgacgcgccg atggcgacgg tcgaggaggg
      841 cgtttcccaa tatatcgact ggttgcacgc ccatgtataa aaccctgcag gattttgtcg
      901 gaaacacgcc gctggttcgc ctgcagcgca ttcccggcgt tgataacgaa cgccgtggca
      961 acgtcatcct ggccaagctc gaaggcaaca atccggccgg ttcggtcaag gatcggccgg
     1021 cgctgtcgat gatccagcgc gccgaggcgc gtggtgacat caagccgggc gacacactga
     1081 tcgaagcgac ctccggcaac accggcatcg cgctggcgat ggcggcggcg atcaagggtt
     1141 acaagatgat cctggtgatg cccgagaacc agagcatcga gcgccgccag agcatgcgcg
     1201 cctacggcgc cgaactggtg ctgacgccca aggacggcgg catggaactg tgccgcgacg
     1261 tcgccgaacg catgcgcgac gaaggcaagg gcatcatcct cgaccagttc gccaacccgg
     1321 acaatccggt cgcccactac gagggcaccg gcccggaaat ctggcgtgac accggcggcc
     1381 gtatcagcca tttcgtgtcg agcatgggca ccaccggcac gatcatgggc accagccgct
     1441 acctcaagga aatgaacccg gcgatcacca tcgtcggctg ccagccggac gaaggcagcc
     1501 agattcccgg catccgcaag tggccggaag cctacctgcc gaagatctac gcgccgacca
     1561 atgtcgaccg catcgaatac gtcagccagg ccgatgccga ggcgatgacc cggcgccttg
     1621 ccgtcgagga aggcatcttt gccggcattt cgtccggcgg cgcgctcgcc gtggcgctgc
     1681 gcatcgccgc ccaggtcgag aatgcgacca tcgtctcgat cgtctgcgac cgcggcgacc
     1741 gctacctgtc gaccggcgtc ttccccgcat gaagccggtc ctcgtcttcg acatcgaaac
     1801 cattcccgac gttgccggcc tgcgccggat caacgagttg ccgggcgaac tgtccgacgc
     1861 cgaagtcgcc gaactcgcct tccagcagcg ccgggcgaag aacggcagcg atttcctgcc
     1921 gctgcacctg cagcgcatcg tcgccatctc ctgcgtactg cgcacgctcg acggtttccg
     1981 cgtctggtcg ctggccgagc cggagcttga cgaaggcgcg atcatccagc gtttcttcga
     2041 cggcatcgag aaattcacgc cgcagatcgt ctcctggaac ggcggcggct tcgacctgcc
     2101 ggtgctgcac taccgcggca tgctgcacgg cgtcacggcc ccgcgctact gggacatggg
     2161 cgacggcgac tacgccgatt cgcgcgactt caagtggaac aactacatca gccgctacca
     2221 cacccgtcac ctcgacttga tggacctctt ggcgatgtac aacggtcgcg ccaacgcgcc
     2281 gctcgacgaa ctggccaagc tgttcggctt tcccggcaag ctcggcatgg acggcagcaa
     2341 ggtctgggag gcctggcagg ccgggcaggc cgccgacatc cgcgattact gcgagactga
     2401 tgtggtcaat acctacctgg tctataaccg tttccggcgc atgcgcggcg aatttaccgc
     2461 cgacgaggaa atggccgaac aggctttcat caagggccaa ctggcgaaga tcgatgcgcc
     2521 gcactggcgc gagtttctcg ccgcctggca atgatcatgc ggcgcagcaa gctataatcg
     2581 caaggttttt tttcaaaccc aacgcagaga gaaaataatg gctcttaaca acgttccctc
     2641 cggcaagtcc ctccccgacg acttcaacgt catcatcgag atcaccgccc attccgaccc
     2701 ggtcaagtac gaagtcgaca aggaaagcgg cgccatcttc gtcgatcgct tcatgtcgac
     2761 cgccatgcac tatccgtgca actacggcta cattccgcac accatcgctg gcgacggtga
     2821 tccggtcgac gttctggtga tcagcccgtt cccgctgccg ccgggcgtcg tcgtccgctg
     2881 ccgcccgctc ggccagctat cgatgaccga cgaaggcggc gaagacgcca agctgctcgc
     2941 cgttccggtg tccaagctga ccccgctgta caacgacgtc aagacctacg aagacacgcc
     3001 ggaactgctg cgccagcaga tcgcgcactt cttcgagcac tacaaggacc tggaacccgg
     3061 caagtgggtc aaggtcaatg gctggcaagg tatcgacgcc gcccgcgaag aagtcatgac
     3121 cggcgtccag cgttacaacg aagcgaaaga caagccgaac tactgagatg ctgcgtatcg
     3181 ccgttgccca gttcaacgcc gtcgtcggtg acctgaccgg caacgtcgac cgcatcgtcg
     3241 cctgcgctgt cgcggccaag gcccgtggcg cgcaggtgct gctgacgccg gaattggcgt
     3301 tgagcggcta tccgccggaa gacctgctgc tgcgcccgga tttctaccgc gcctgccagc
     3361 gggcgcttga tgatctggcc gcccgcatcg acgggattgc gctggtcgtc ggccacccgg
     3421 aagaacatga aggcgcctgc tacaacgccg cgacggtcat cgaaaacggc cgtccgcttg
     3481 ccgtctaccg caagatgcgc ctgcccaatt acgacgtctt cgacgagaag cgctatttcg
     3541 acgccgggcg c
//
LOCUS       DAOSDX010000200         3548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9283_length_3548_cov_2.795877, whole genome shotgun sequence.
ACCESSION   DAOSDX010000200 DAOSDX010000000
VERSION     DAOSDX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9283_length_3548_cov_2.795877"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLN02_05610"
     CDS             <1..277
                     /locus_tag="PLN02_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOI51834.1"
                     /translation="VGDLTRYTQYQVASSIPDAARNEGGLAAMGAGLGFGQMVGQAVA
                     AATPAAVAVNADEVVATLEKLHALMGKGILSQEEFDAKKAELLKKLG"
     gene            complement(286..936)
                     /locus_tag="PLN02_05615"
     CDS             complement(286..936)
                     /locus_tag="PLN02_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51835.1"
                     /translation="MKFVLLAAVLIGAWLAWRKWRQAGRALFIERFPLQEKLDRRLAA
                     RRPELDAAQRSEVFAGLRDYFLICNNAGRRMVAMPSQAVDDAWHEFILFTRHYRQFCN
                     RAFGRFLHHTPAEAMRSPTEASEGLKRAWRLACAHEGIDPKQPARLPRLFALDAALGI
                     AGGFVYQLDCLAAGGTGRDGYCASHIACSSGCSGDSGSSDGDSGGGCGGGCGGGGD"
     gene            complement(936..2387)
                     /locus_tag="PLN02_05620"
     CDS             complement(936..2387)
                     /locus_tag="PLN02_05620"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HOI51836.1"
                     /translation="MNLQNPTLLRTANLIDGLWVGADGGATLSVVDPASGERIAEVPC
                     CGAAETQRAIAAADAALPAWRALTARRRGQLLQNWNRLILDNADDLAALITAEGGKPL
                     LEAKGETIYGASFVEWFAEEGKRTYGESIPSPASTTRLLVVKQPIGVCAAITPWNFPL
                     AMITRKVAPALAAGCTVVVKPAEATPLTALALAALAEQAGFPAGVFNVVTGQPAAIGG
                     ELCANPTVRKLSFTGSTAVGRLLMAQCAPTVKKLSLELGGNAPFIVFDDADVEAAVEG
                     ALAAKYRNTGQTCVCANRFLVQSGIYEAFAARFAERSAQLKVGAGSEAGVAQGPLINA
                     AGLAKVEAHVADAVAKGARVLCGGARHERGGNFFQPTVLADVTPAMRVAREETFGPLA
                     PLFRFDTEAEAVAMANDTEFGLAAYFFSRDVGRCWRVGEALEYGMVGVNTGMISNEVA
                     PFGGIKQSGIGREGSKYGIEEYLEVKYLCFDVR"
     gene            2450..2941
                     /locus_tag="PLN02_05625"
     CDS             2450..2941
                     /locus_tag="PLN02_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOI51837.1"
                     /translation="MRRFFCWLSLLVCLALAACGSPGPRGGSTITQAPRAVSGQASEV
                     VMLAMGLIDTGYRFGGKNPEAGFDCSGMVAYVFDKAVGVKVSGSAADIARRGRAVGRH
                     ELQPGDLVFFNTMNRPFSHVGIYIGEQRFVHAPSTNGRVRIERLDSEYFARRYEAART
                     LLD"
     gene            3410..>3548
                     /locus_tag="PLN02_05630"
     CDS             3410..>3548
                     /locus_tag="PLN02_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOI51838.1"
                     /translation="MSTVTLAEAKTHLSHLLDQVEAGEEVVITRRGLPIARITPVEKP
                     KQ"
BASE COUNT          591 a         1161 c         1204 g          592 t
ORIGIN      
        1 ggtcggcgac ctgacgcgct acacgcagta ccaggtggcg agcagcattc ccgacgcggc
       61 gcgcaacgaa ggcgggctgg cggcgatggg cgccggtctc ggcttcggcc agatggtcgg
      121 ccaggcggta gccgcggcga cgccggcggc ggttgcggtc aatgccgacg aggtggtggc
      181 gacgctggaa aagctgcatg cgctgatggg caagggcatc ctgtcccagg aagaattcga
      241 cgccaagaaa gcggaactgc tgaagaagct cggctgaacg cggcttcagt cgccaccacc
      301 gccgcatccg ccgccacagc ccccgccact gtcgccgtcg ctgctgcccg aatcgccgct
      361 gcagccgctg ctgcaagcga tgtggctggc gcagtagccg tcgcggccgg taccgccggc
      421 ggccagacag tccagttggt agacgaagcc gccggcgatg cccagcgccg cgtcgagcgc
      481 gaacaggcgc ggcaggcgcg ccggttgttt cggatcgatc ccctcgtgcg cgcaggccag
      541 ccgccaggct cgcttgagac cctcgctcgc ctcggtcggc gagcgcatcg cctcggccgg
      601 cgtgtgatgc agaaagcggc cgaaggcacg gttgcagaac tgccggtagt ggcgggtaaa
      661 caggatgaac tcgtgccagg cgtcgtcgac cgcctgcgac ggcatcgcca ccatccggcg
      721 gccggcgttg ttgcagatca ggaagtagtc gcgcaggccc gcgaagactt cgctgcgctg
      781 cgcggcatcg agttccgggc ggcgtgccgc caggcgccgg tcgagttttt cctggagcgg
      841 gaagcgttcg atgaagagcg cccgtccggc ctgtcgccat ttgcgccagg ccagccaggc
      901 gccgatcagc acggcggcga gcaggacgaa cttcatcagc gcacgtcgaa acagaggtat
      961 ttcacctcaa ggtattcctc gatgccgtac ttcgaccctt cgcggccgat ccccgactgc
     1021 ttgatgccgc cgaagggcgc cacttcgttc gagatcatcc cggtgttgac gccgaccatg
     1081 ccgtactcca gcgcctcgcc gacgcgccag cagcggccga cgtcgcggct gaagaaatag
     1141 gcggcgaggc cgaactcggt gtcattggcc atcgccaccg cttctgcctc ggtgtcgaag
     1201 cggaaaagcg gcgccagcgg gccgaaggtt tcctcgcggg cgacgcgcat cgccggcgtc
     1261 acgtcggcca gcacggtggg ctggaagaag ttgccgccgc gctcgtggcg ggcgccgccg
     1321 cacagcacgc gggcgccctt ggcgacggcg tcggcgacgt gggcctcgac cttggccagg
     1381 ccggcggcgt tgatcagtgg cccctgggcg acgcccgctt cgctgccggc gccgaccttg
     1441 agctgcgcgc tgcgctcggc gaaacgggcg gcgaaggctt cgtagatgcc cgactggacc
     1501 aggaagcggt tggcgcagac gcaggtctgg ccggtgttgc ggtacttggc ggcgagcgcg
     1561 ccttcaaccg cggcctcgac gtcggcgtcg tcgaagacga tgaagggggc gttgccgccc
     1621 agttcgagcg acagtttctt gacggtcggc gcgcactgcg ccatgagcag ccggccgacc
     1681 gcggtcgagc cggtgaagga gagtttgcgc acggtcggat tggcgcacaa ttcgccgccg
     1741 atcgccgccg gttggccggt gacgacgttg aagacgccgg ccggaaaacc cgcctgctcg
     1801 gccagcgcgg ccagcgccag ggcggtcagc ggcgtcgcct cggccggttt gacgacgacc
     1861 gtgcagccgg cggcgagcgc cggcgccacc ttgcgcgtga tcatcgccag cggaaaattc
     1921 cacggcgtga tcgcggcaca gacgccgatc ggctgcttga ccaccagcag ccgggtagtc
     1981 gacgccgggc tgggaatgct ctcaccgtag gtgcgcttgc cttcctcggc gaaccattcg
     2041 acgaaggagg cgccgtagat cgtctcgccc ttcgcctcga gcagcggctt gccgccttcg
     2101 gcggtgatca gggcggcgag atcgtcggca ttatcgagga tcaggcggtt ccagttctgc
     2161 agcaactgcc cgcggcggcg agcggtgagc gcccgccagg ccggcagcgc ggcgtcggcg
     2221 gcggcgatcg cgcgctgcgt ctcggcggcg ccacagcagg gaacctcggc gatgcgttcg
     2281 ccgctggccg ggtcgacgac gctcagcgtg gcgccgccgt cggcgccgac ccatagacca
     2341 tcgatcaggt tggcggtgcg caggagagtc gggttctgca agttcatggg cttgaaaaat
     2401 cctgtaaaaa cgttaagtta cgaataactt caagaaaagg ctttaagcga tgcgtcgatt
     2461 tttctgctgg ttgtcgctgc tcgtctgcct ggcgcttgct gcctgcggca gtcccgggcc
     2521 acgcggcgga tcaactataa cccaggcgcc gcgggcggtc agcgggcaag ccagcgaggt
     2581 cgtcatgctg gccatggggc tgatcgacac cggctaccgc ttcggcggca agaacccgga
     2641 agccggattc gactgcagcg gcatggtcgc ctacgtgttc gacaaggcgg tcggcgtcaa
     2701 ggtcagcggc agcgccgccg acatcgcccg ccgcggccgt gccgtcggtc gccacgaact
     2761 gcagcccggc gacctggttt tcttcaacac gatgaatcgc ccgttctcgc acgtcggcat
     2821 ctatatcggc gagcagcgtt tcgtacacgc gccatcgacc aacggacggg ttcgcatcga
     2881 acgtctcgac agcgagtact tcgcgcgccg ctacgaagcc gcgcgcacct tgcttgattg
     2941 aaagcagcgc gagtcatgga cgcgcttccg ccgatgaggt tacaattgcg gcctttccgc
     3001 ccgtagctca gctggataga gtactgccct ccgaaggcag gggtcgtgcg ttcgaatcgc
     3061 gccgggcgcg ccatatatca aggggtttgg ccttgcgggt caggcccatc ctcgtctgtg
     3121 cccatcccgt gcccaatctg tgccttgttc gtgtctacct catgtgcggc acagatgcgt
     3181 gtcgcgtttt ttgacgagtg cttcccatgc ctggcctcag gatttgtcgc cgttcgcgac
     3241 gtattcaaaa agatctccca ccccgcaccc aaacaatcgg catagctgat cgatagtttc
     3301 caagtcgatg cgggtcgcgg cctcgttgca gatgactaac agtggcgatt tactagcccc
     3361 acccattgcg ctactatgtg accaacataa tgactaaccg gaggggtgta tgagtaccgt
     3421 taccctggca gaagccaaga cccatctcag ccatcttctc gaccaggtcg aagcgggtga
     3481 ggaagtggtc atcacccgtc gtggcctgcc gattgcccgc atcacgccgg tcgagaagcc
     3541 caagcagg
//
LOCUS       DAOSDX010000201         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9308_length_3540_cov_3.365567, whole genome shotgun sequence.
ACCESSION   DAOSDX010000201 DAOSDX010000000
VERSION     DAOSDX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9308_length_3540_cov_3.365567"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLN02_05635"
     CDS             <1..347
                     /locus_tag="PLN02_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI51839.1"
                     /translation="AMRERAAALLAGVGLADRANHPATKLSGGQQQRVAIARALAMRP
                     ALVLADEPTGNLDTHTADDIFALLREVNQREGTSFLIVTHDPRLAARCDRIVELVDGS
                     IVSDRPNPETPG"
     gene            406..1545
                     /locus_tag="PLN02_05640"
     CDS             406..1545
                     /locus_tag="PLN02_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOI51840.1"
                     /translation="MPALLPHPDPDGLLEYSVVYTDRALNHMSQRFQGVMREISRILK
                     KAYNAKASVLVPGGGTYGMEAVARQFATNRKVLVIRNGFFSFRWTQIFDMGRIPSESI
                     VLKARPQGSGVQAPFAPPPLAEVIATIERERPEVVFAPHVETSSGMILPDDYLRAVGA
                     AVRAVDGLFVLDCIASGTVWVDIAANNVDVLISAPQKGWSGPSCCALIALGERARARI
                     DATTSSSFACDLKKWLQIMETYENSGHAYHATMPTDALAAVCQAMQATEARGFAQVCA
                     EQWELGRQARALLVERGFPSVAAEGFEAPGVVVSYTTDPDIQNGRKFLAQGIQSATGV
                     PLMCDEPEGFRTFRLGLFGLDKLQAPTRTVASLASALDAFPPLAD"
     gene            1642..2157
                     /locus_tag="PLN02_05645"
     CDS             1642..2157
                     /locus_tag="PLN02_05645"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOI51841.1"
                     /translation="MKNLFDITSKRFLAASALALAVPLSAMAFQGGKGGCDARDGMRG
                     ASMKQGAMMHGGGMMAMRGLHRLNLSEAQDDKIFEIMHAQAPKMREQMKSLRKSEDEL
                     RALKAAPDFSEAKAKQLVDRIAGQRADMEMARLQTERQIMGLLTPEQQKQLADMKQRG
                     PGKRDDRGPRS"
     gene            complement(2233..2784)
                     /gene="phaR"
                     /locus_tag="PLN02_05650"
     CDS             complement(2233..2784)
                     /gene="phaR"
                     /locus_tag="PLN02_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HOI51842.1"
                     /translation="MSEPVRLIKKYPNRRLYDTKTSAYITLGDVKELVLKFEPFKVLD
                     AKSGEDLTRSILLQIILEEETGGVPLFSCELLSGFIRFYGSTSQSMLGKFLENNMKTF
                     VDFQQKVQEQSRSLYGGSDTASVQADFWNQFLTFQQPAMQNMMTAYMDQSKKMFQSMQ
                     DQLQSQTRTMFGGLGTPDVPEKK"
     gene            complement(2880..>3540)
                     /gene="phbB"
                     /locus_tag="PLN02_05655"
     CDS             complement(2880..>3540)
                     /gene="phbB"
                     /locus_tag="PLN02_05655"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HOI51843.1"
                     /translation="KVVAAYHPQFDNKDAWLAEMSAAGFNDFVCVAGDVSVLEDCQKM
                     VAEAEAACGQVDILINNAGITRDRMFAKLEKDGWDAVISTNLTSLFNMTKQVSAKMAE
                     RGWGRIINISSVNGVKGQAGQTNYSAAKAGVIGFTKALAAELAAKGVTVNAIAPGYVA
                     TKMVMAIKPEVLETIVASVPMKRLAKPEEIGFACAYLSDELSGFTTGATINVNGGLYY
                     Q"
BASE COUNT          644 a         1182 c         1114 g          600 t
ORIGIN      
        1 cagcgatgcg cgagcgggcg gccgcgctgc tcgccggcgt cggcctcgcc gatcgagcca
       61 accacccggc gacgaaactc tccggcggcc agcagcagcg cgtggccatc gcccgcgcgc
      121 tggccatgcg gccggcgcta gtgctcgccg acgagccgac cggcaacctc gacacccata
      181 ccgccgacga catcttcgcg ctgctgcgtg aggtgaacca gcgcgaaggc accagcttcc
      241 tgatcgtcac ccacgacccg cgactggccg cacgctgcga ccgcatcgtc gaactggtcg
      301 acggcagcat cgtcagcgac cggccgaacc cggaaacgcc gggctagccg gcacggccgg
      361 cgggcgttat cctcgcaagc tgctcaacgc caactggaga acaccgtgcc cgccctgctc
      421 ccccatcccg atcccgacgg cctgctcgaa tattccgtgg tctacaccga ccgcgcgctg
      481 aaccacatgt cgcagcgttt tcagggcgtc atgcgggaaa tctcgcgcat cctcaagaaa
      541 gcctacaacg ccaaggccag cgtgctcgtc cccggcggcg gcacctacgg catggaagcg
      601 gtggcccgcc agttcgccac gaatcgcaag gtgctggtga tccgcaacgg cttcttcagc
      661 ttccgctgga cgcagatctt cgacatgggc cgcattccgt ccgagagcat cgtcctcaag
      721 gcgcgcccgc agggcagcgg ggtgcaggcg ccgttcgcgc cgccgccgct cgccgaggtg
      781 atcgccacca tcgaacgcga acgccccgaa gtggtgttcg cgccgcacgt cgaaacctcg
      841 tcgggcatga tcctgcccga cgattacctg cgcgccgtcg gcgccgcggt gcgcgccgtg
      901 gacggcctgt tcgtgctcga ctgcattgcc tccggcacgg tctgggtgga cattgcggcg
      961 aacaacgtcg acgtcctgat cagcgccccg cagaagggct ggagcggccc ctcgtgctgc
     1021 gccctgatcg ccctcggcga acgcgcccgg gcgcgcatcg acgcgacgac cagcagcagc
     1081 ttcgcctgcg acctgaagaa atggctgcag atcatggaaa cctacgagaa cagcggccac
     1141 gcctatcacg cgacgatgcc gaccgacgcc ctggccgccg tctgccaggc gatgcaggcg
     1201 accgaggcgc gcggcttcgc gcaggtctgc gccgaacagt gggaactcgg ccgacaggcg
     1261 cgcgccctgc tcgtcgaacg cggtttcccc agcgtcgccg ccgagggctt cgaggcaccg
     1321 ggcgtcgtcg tcagctacac caccgacccg gatatccaga acggccgcaa gttcctcgcc
     1381 cagggcatcc agagcgccac cggcgtgccg ctgatgtgcg acgagccgga gggtttcaga
     1441 acatttcgcc tcggcctctt cggtctcgac aagctgcagg cgccgacgcg cacggtggcc
     1501 agcctggcca gcgcgctcga cgccttcccg ccgctcgccg actgaaatga atccttacat
     1561 ctggaaaacg acagctaacg ccaatctccg taacctgctc ctgtcgccgg aacgtttccg
     1621 gcttaccgac aggagaacat catgaagaac cttttcgaca tcaccagcaa acgcttcctc
     1681 gccgccagtg cccttgccct cgccgttccg ttgagcgcaa tggccttcca gggcggcaaa
     1741 ggcggctgcg atgcgcgcga cggcatgcgc ggcgcgtcga tgaaacaggg agcgatgatg
     1801 cacggcggcg gcatgatggc gatgcgcggc ctgcaccggc tcaacctgag cgaagcgcag
     1861 gacgacaaga tcttcgagat catgcacgcc caggcgccga agatgcgcga gcagatgaag
     1921 tcgctgcgca agtcggaaga cgagctgcgc gccctcaagg ccgcccccga cttcagcgaa
     1981 gccaaggcca agcagttggt cgaccgcatc gccggccagc gcgccgacat ggagatggcg
     2041 cgcctgcaga ccgagcggca gatcatgggc ctgctgacgc cggaacagca gaagcagctc
     2101 gccgacatga agcagcgcgg accgggcaag cgcgacgacc gcgggccgcg ttcctgagcg
     2161 tcagccagcc ccgacagcag aacggccgcg aatgcggccg ttttttttct ggcgccggga
     2221 aaaccttccc ggctatttct tttccggcac gtccggcgtt cccagaccgc cgaacatcgt
     2281 ccgcgtctgc gactgcaact ggtcctgcat cgactggaac attttcttcg actggtccat
     2341 gtaggccgtc atcatgttct gcatcgccgg ctgctggaag gtcaggaact ggttccagaa
     2401 atcggcctgc acgctcgccg tgtccgagcc gccgtacagc gagcgggact gctcctgcac
     2461 cttctgctgg aaatcgacga aggtcttcat gttgttctcg aggaacttgc cgagcatgct
     2521 ctgcgaggtg ctgccgtaga aacggatgaa gccggagagc agttcgcagg agaacagcgg
     2581 tacgccgccg gtttcctctt ccaggatgat ctgcagcagg atgctgcggg tcaggtcctc
     2641 gccggacttg gcatcgagca ccttgaacgg ttcgaacttg agcaccagtt ccttgacgtc
     2701 ccccagtgtg atgtatgcgc ttgtcttggt atcgtagagg cggcgattgg ggtatttctt
     2761 gatcaggcga accggttcgg acatgctgga gcctcaagag aaaatttatg tgcagcgcag
     2821 cattataacg gaaaaaaaga gcgccccaag gcgccctttt cgcttcctgc gcgggcttct
     2881 tactggtagt agaggccgcc gttgacgttg atcgtcgcgc cggtggtgaa gccggacagt
     2941 tcgtcggaca ggtaggcaca ggcgaagccg atttcttccg gcttggccag gcgcttcatc
     3001 ggcaccgagg cgacgatggt ttccagcact tccggcttga tcgccatgac catcttggtc
     3061 gccacgtagc ccggggcgat cgcgttgacg gtgacgccct tggcagccag ttcggcggcc
     3121 agcgccttgg tgaagccgat gacgccggcc ttggcagccg agtagttggt ctggccagcc
     3181 tgacccttga cgccgttgac cgacgagatg ttgatgatgc ggccccagcc acgttcggcc
     3241 atcttggcgg aaacctgctt ggtcatgttg aacagcgagg tcaggttggt cgagatgacg
     3301 gcatcccagc catccttttc cagcttggcg aacatgcggt cacgggtgat gccggcattg
     3361 ttgatcagga tgtcgacctg gccgcaggcg gcttcggctt cggcaaccat cttctgacag
     3421 tcttcgagga cggaaacgtc accggcgacg cagacgaaat cgttgaaacc ggcagcggac
     3481 atctcggcca gccaggcgtc cttgttgtcg aattggggat ggtaggcggc gaccaccttg
//
LOCUS       DAOSDX010000202         3525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9364_length_3525_cov_2.993660, whole genome shotgun sequence.
ACCESSION   DAOSDX010000202 DAOSDX010000000
VERSION     DAOSDX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9364_length_3525_cov_2.993660"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLN02_05660"
     CDS             <1..639
                     /locus_tag="PLN02_05660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI51844.1"
                     /translation="AGEGVPGVRVVGRGVDTTLFDPARRSNELRARWGADGAGPVCLY
                     VGRLAPEKNLALVEKCFAALQVWHPAARLVWVGDGPSARKLEQDHPDQYFAGMRTGED
                     LAAHFASADLFLFPSLSETYGNVVAEAMASGVPVVAYRSAAAAELVADGDNGRVVAPG
                     DERAFVEAALWALEDGERLRRMGEQARMAMLPRGWPDVVASFERAVREALAG"
     gene            complement(644..>1400)
                     /locus_tag="PLN02_05665"
     CDS             complement(644..>1400)
                     /locus_tag="PLN02_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOI51845.1"
                     /translation="LQARNLGQALELLLRYQAWLSPLLAPHLMRQGEQTLLYWTDSCV
                     SARQRTFLVEMMMAAVSGMARWLSGQRLPWQYSFNRTRPAHPEQYAVHLGSQVQFNAY
                     LDAMAIASHWLEQPWPRGSEMGVQMALAAAEREIGQQPRRSLLAGLYDYLHEHIRQSP
                     SLEQCSAALGTSPATLKRHLAAHDTHFQAELDQVRTHVTLELIHNRGYSNEQIAAHLG
                     FHDANNFRRSFKRWTGLTPSVFRSHLFQLQASE"
     assembly_gap    1401..1410
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1411..1680)
                     /locus_tag="PLN02_05670"
     CDS             complement(<1411..1680)
                     /locus_tag="PLN02_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51846.1"
                     /translation="MHCYQLHSPCIPAAYQPALTLEFARNQELDYRDLLPGTGLTAAD
                     LAAQSLTPAGYLRLLHNLATALPDGDTAFLLGQTLLPGHQGPISHA"
     gene            complement(1685..2587)
                     /locus_tag="PLN02_05675"
     CDS             complement(1685..2587)
                     /locus_tag="PLN02_05675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOI51847.1"
                     /translation="MHQPYRNALIAMSLVAGACLLLYGSLGQAKPMAIWNWMDIVGEG
                     GTALMSALWALLILGSRPGGRVTLLLAGGLSAIMLGAWVDCLDEFFSIPDQQLWDNWL
                     ESLLTPGGMLTLTLGLYFWRQEQWSLNEHMQKRERLFREHRSFDRITQLANADYLRRQ
                     LRLEEERDPGGSALILLDVDDFHLINREQGQQEGNRLLQAISHLLLLNLRPQDLLCRY
                     AGDRYAILLPAASAATAATMARHLQQAVRGLAHHTRNGTRLRLSGRVAWASLEADTDA
                     LLSRLDRALEGEPAIPEGGPAVAV"
     gene            2922..>3525
                     /locus_tag="PLN02_05680"
     CDS             2922..>3525
                     /locus_tag="PLN02_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI51848.1"
                     /translation="MYRKVNAVNAGPADWRPRTIALAIGLALAGPAIAQEAVPVMQEV
                     LVTGQAASMDSALDVQQMADNIVSVVHADAIGQLPDSNAAEALQRIPGLSVERDQGEG
                     RYVRVRGLGPELNSVTINGSLVPAPESDTRAVALDVLLAGLIRSLEVSKSLTPDQDAN
                     SIGGTIEVKTISAFDHKGMFYSLEGGASYDSNVEQTSPWFA"
BASE COUNT          538 a         1061 c         1330 g          586 t
ORIGIN      
        1 gccggcgagg gcgtgcccgg cgtgcgcgtc gtcggtcgcg gcgtcgatac cacgctcttt
       61 gaccccgccc gccgcagcaa cgaactgcgc gcgcgctggg gggcggacgg cgcggggccg
      121 gtctgcctct acgttggccg gctggccccg gagaagaatc tggcgctggt cgagaaatgc
      181 tttgccgcgc ttcaggtctg gcatccggcg gcgcgcctgg tgtgggtcgg cgacgggccg
      241 tcggcgcgca agctggagca agatcacccg gatcagtact tcgccggcat gcgcaccggc
      301 gaggacctgg cggcgcattt cgcgagcgcc gacctcttcc tctttcccag tctcagtgag
      361 acctacggca acgtggtggc ggaggcgatg gccagcggcg tgccggtggt tgcctaccgc
      421 agcgcggcgg cggccgagct ggtggctgat ggcgacaacg gccgcgtcgt cgccccgggc
      481 gacgaaaggg ccttcgtcga ggcggcgttg tgggcgctgg aagacggcga acgcctgcgc
      541 cggatgggtg agcaggcgcg catggcgatg ctgccgcgcg gctggccgga cgtcgtcgcc
      601 agtttcgagc gggcggtgcg cgaggcgttg gcgggctgag cgcttattcg ctggcttgca
      661 gttgaaacag atggctgcgg aagaccgacg gcgtcagtcc ggtccagcgc ttgaacgagc
      721 gacggaaatt gttggcgtcg tgaaagccga ggtgggcggc gatctgttcg ttgctgtagc
      781 cgcggttgtg gatcagttcc agcgtgacat gggtgcgcac ctggtccagt tccgcctgaa
      841 agtgggtgtc atgggcggcg agatggcgtt tcagcgtcgc cgggctggtg cctagcgcgg
      901 cgctgcattg ttcgaggctg ggcgactggc ggatatgttc gtgcaggtaa tcgtagagtc
      961 cggccagcag gctgcgccgg ggctgctggc cgatctcccg ttccgctgcc gccagcgcca
     1021 tctgcacgcc catctcgctg ccgcgcggcc agggctgctc cagccagtgg ctggcgatgg
     1081 ccatggcgtc cagataggcg ttgaactgca cctgactgcc cagatgcacc gcgtattgct
     1141 cgggatgggc ggggcgggtg cggttgaagc tgtactgcca gggcaggcgc tggccgctca
     1201 gccagcgcgc catgccgctg accgccgcca tcatcatttc gaccaggaaa gtccgctgcc
     1261 gggcgctgac gcaactgtcg gtccagtaga gcagggtctg ctccccctgg cgcatcaggt
     1321 gcggcgccag cagggggctg agccaggcct ggtaacgcag cagcagttcc agcgcctggc
     1381 ccaggttgcg cgcctgcagc nnnnnnnnnn tgcgtggctg atcggtccct ggtggccggg
     1441 caacagggtc tggccgagca ggaaggcggt gtcgccgtcg ggcagggccg tcgccaggtt
     1501 gtgcagcagg cgcagatagc cggccggggt cagcgactgg gcggccagat cggccgccgt
     1561 cagccccgtg ccgggcagca gatcgcggta gtcgagctcc tggttgcggg cgaattccag
     1621 ggtgagggcg ggctggtagg cggcgggaat gcagggactg tgcagttgat agcagtgcat
     1681 gacgtcacac ggcgaccgcc ggcccgccct ccgggatcgc cggttcgccc tccagcgccc
     1741 ggtcgagccg gctgagcagg gcgtcggtgt ccgcttccag cgaggcccag gcgacccggc
     1801 cggagaggcg cagccgggtg ccgttgcggg tgtggtgggc gaggccgcgc accgcctgtt
     1861 gcaggtggcg ggccatggtg gcggcggttg ccgcgctggc tgccggcagc aggatggcgt
     1921 agcggtcgcc ggcatagcgg cagagcaggt cctgcgggcg caggttgagc agcagcagat
     1981 ggctgatcgc ctgcagcagg cggttaccct cctgctggcc ctgctcccgg ttgatcaggt
     2041 ggaagtcatc gacgtcgagc aggatcagtg cgctgccgcc gggatcgcgc tcctcttcga
     2101 gacgcaactg gcggcgcagg taatcggcgt tggccagctg ggtgatgcgg tcgaaggagc
     2161 ggtgttcgcg gaacaggcgc tcgcgtttct gcatgtgctc gttgagcgac cactgttcct
     2221 ggcgccagaa atacagcccc agggtcaggg tcagcatgcc gccgggcgtg agcagggatt
     2281 ccagccagtt gtcccagagc tgctggtcgg gaatgctgaa gaattcgtcg aggcaatcga
     2341 cccaggcgcc gagcatgatc gccgacaggc cgccagcgag cagcagcgtg acccggccgc
     2401 cgggtcggct gcccaggatg agcagcgccc acagggccga catcaacgcg gtgccgcctt
     2461 cgccgacgat gtccatccag ttccagatgg ccatcggctt ggcctggccg agactgccgt
     2521 agagcagcag gcaggcgccg gcgaccaggg acatggcgat cagggcattg cgatagggtt
     2581 ggtgcatggc ggatggtgac gctcgggaaa accatcatgc taggcaggtc gtgtgacagc
     2641 ccggttgcag ggcggcggat cgggcgggag ggggtgaaat cggctcaagg acgcggggtt
     2701 ctggcgctgg gcgccaggca ttcgcctgcc ggcacggcat gccggggcag cggatcgacg
     2761 cttcggggct gtcgccgggg caaggcgaaa cggctcagtt tttcctgggg tgctatctaa
     2821 gtcattgatt tttaaatttt cgtgcttggg cttgtcacgg atttgcaatc ttggccgcct
     2881 agcctgcgct ccgttctgac atatcaacgg ggagcaggaa gatgtacagg aaagtcaatg
     2941 cagtgaatgc cggaccggcg gactggcggc cgcggacgat cgcactggcg atcgggctgg
     3001 cgctggccgg tccggccatc gcccaggaag ccgtgccggt gatgcaggaa gtcctggtga
     3061 ccggccaggc ggccagcatg gacagcgcgc tggatgtgca gcagatggcc gacaacatcg
     3121 tcagcgtggt gcacgccgac gccatcggcc agttgccgga cagcaacgcc gccgaagccc
     3181 tgcagcggat tcccggcctg tcggtcgagc gcgatcaggg tgaggggcgt tacgtgcggg
     3241 tgcgtggcct gggaccggaa ctgaacagcg tcaccatcaa cggttcgctg gtgccggcgc
     3301 cggaaagcga cacccgggcg gtggcgctcg atgtcctgct ggccggcctg atccgttctc
     3361 ttgaagtgag caagtcgctg acgccggacc aggacgccaa ttcgatcggc ggcacgatcg
     3421 aggtcaagac catttccgct ttcgaccaca agggcatgtt ctattcgctg gaaggcggcg
     3481 ccagctacga tagcaacgtc gagcagacca gtccgtggtt tgccg
//
LOCUS       DAOSDX010000203         3523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9369_length_3523_cov_3.117647, whole genome shotgun sequence.
ACCESSION   DAOSDX010000203 DAOSDX010000000
VERSION     DAOSDX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9369_length_3523_cov_3.117647"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2591)
                     /locus_tag="PLN02_05685"
     CDS             complement(<1..2591)
                     /locus_tag="PLN02_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOI51849.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIESAAKEQRKSNIYKG
                     VITRIEPSLEACFVDYGADRHGFLPFKEVSRAYFQPGVEANKAKINEALKEGQELIVQ
                     VDKDERGNKGAALTTYVSLAGRYLVLMPNNPRGGGVSRRVDGDERAELREIMDQLEVP
                     QGMSLIARTAAIGRQAEELQWDLNYLLQLWSAIEGAAQQQSGAFLIYLEGSLVIRAIR
                     DYFQPDIGEILIDTDEVHEQARAFMGTVMPGNVNRVKRYSDDVPLFSRFQIEHQIETA
                     FARQVNLPSGGAIVIDHTEALVAVDVNSGRATKGADIEETALKTNLEAADELARQLRL
                     RDLGGLIVIDFIDMENSKAQREVENRLRDALRFDRARVQMGKISRFGLMELSRQRLRP
                     ALAETSYIPCPRCTGTGHIRSTESAALHILRILEEEAMKENTGAVHVQVPVDVATFLL
                     NEKRTDIQAIELRHKVTILLIPNIHLETPAHTITRLRHDDLNNDDLREPSYKMVDLPP
                     DEAEKTGAQADTAKPRQEAAIKGISPEQPAPVIAEKAETPAAVTVTATPATGGLFGWI
                     KSLFVGTPTPAAPVAPSEPQRKPREGRGNADSRRERNDGRRGNNRNNRRDEGDRANRP
                     ERAERPAREENANTAAAGEPRQERRPRKEKRERPQNEAKPVIEQPAPAEVVENSANPA
                     LSTVSPGDDQQAGNRRRGRRGGRGRGERRIDQENQESGLNPAGEVAASAVTAISEDNA
                     TPVEVIIPAAVVATEAAEAIVEAVVAPQTPAEATVPATPEPVVETTTEAVVVEPVAVV
                     VETEPVVAAVETLTVPQTVVEVEAEPVAAPAPVAAAAPVDLDAALSESGLVMVQTTSA
                     ITVAPVEA"
     gene            2989..>3523
                     /locus_tag="PLN02_05690"
     CDS             2989..>3523
                     /locus_tag="PLN02_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOI51850.1"
                     /translation="MNGAGNNSVTHLVIGEEAAGQRLDNYLVRHCKGVPKSHLYRILR
                     SGEVRVNKGRVDATYRLQPGDSLRLPPLRLAERPVDEIAELAKTRVDLPVIYEDEALL
                     VIDKPEGIAVHGGSGVSFGVIEALRRQRPQAKFLELAHRLDRETSGILLVGKKRLALT
                     VLHDMFREHGAGADNRYL"
BASE COUNT          535 a         1016 c         1228 g          744 t
ORIGIN      
        1 gcctcgaccg gtgcaacggt gatggccgag gtggtctgga ccatcaccag gccgctttcc
       61 gaaagcgcgg catcgagatc gacgggggcc gctgctgcta ccggtgcagg tgctgcaacg
      121 ggttcggcct cgacttcgac gacggtttgc gggacggtga gggtttcgac ggcagcaacg
      181 acgggttcgg tttcgaccac cacggccacc ggttctacaa cgacggcctc ggtagttgtt
      241 tcaacgacag gctccggcgt cgccgggact gttgcttcgg caggcgtctg tggcgccacg
      301 acggcttcga cgatggcttc agccgcttcc gttgcgacga cggctgccgg aatgatcact
      361 tcgaccggcg ttgcgttgtc ttcggagatt gcggtcacag cggaagccgc aacttcgcct
      421 gccgggttca ggccgctttc ctgattttcc tggtcgatgc gccgctcacc acggccgcgg
      481 ccaccgcgcc ggccgcgccg acggttgccg gcttgctggt cgtccccggg cgaaacggtg
      541 gagagtgccg ggttggcgct gttctctacg acttcagcgg gtgccggttg ttcaatgacg
      601 ggctttgctt cgttttgcgg acgctcgcgc ttttccttgc gcgggcgacg ttcctggcgt
      661 ggttcgccgg cggcggcagt gtttgcgttt tcttcgcggg cggggcgttc ggcgcgttcc
      721 gggcggttgg cgcgatcgcc ttcatcgcga cgattgttgc ggttgttgcc ccgccggcca
      781 tcgttgcgct cgcgccggct atcggcgttg ccacgacctt cgcggggttt gcgctggggc
      841 tcgctcgggg cgacgggggc cgccggtgtt ggcgtaccga cgaaaagcga tttgatccag
      901 ccgaacaggc cgcctgttgc gggagtggcc gtgacggtaa ctgctgccgg cgtttcggcc
      961 ttctcggcaa tcacgggggc cggctgttcc ggcgaaattc ccttgattgc tgcttcctgg
     1021 cgcggcttgg cggtgtcggc ctgggcgccg gtcttttccg cttcgtcagg cggcaggtcg
     1081 accatcttgt aggacggctc acgcaggtcg tcgttgttca ggtcgtcgtg gcgcagacgg
     1141 gtaatggtat gggccggcgt ttccagatgg atgttaggga tcagcaggat ggtgaccttg
     1201 tggcgcagct cgatcgcctg gatgtcggtg cgcttctcgt tgagcaggaa agtggcgaca
     1261 tcgaccggga cctggacatg cacggcaccg gtgttttcct tcatcgcttc ctcttcaagg
     1321 atgcgcagga tgtgcagcgc ggccgattcg gtcgaccgga tgtggccggt gccggtgcag
     1381 cgcgggcaag ggatgtagct ggtttcggcg agcgccggac gcaggcgctg gcgcgatagt
     1441 tccatcaggc cgaaacggct gatcttgccc atctggacgc gggcgcggtc gaaacgcagg
     1501 gcgtcgcgca ggcggttttc gacctcgcgc tgggccttgc tgttctccat gtcgatgaag
     1561 tcgatgacga tcaggccgcc gaggtcgcgc agacgcaact ggcgggccag ctcgtcggcg
     1621 gcttcgaggt tggtcttgag cgcggtttcc tcgatgtcgg cgcccttggt ggcgcgaccg
     1681 gagttgacgt cgacggcgac cagggcttcg gtgtggtcga tgacgatggc gccgccggac
     1741 ggcaggttga cctggcgggc gaaggcggtc tcgatctggt gctcgatctg gaagcgcgag
     1801 aacagcggta cgtcgtcgct gtagcgcttg acgcgattga cgttgccggg catcacggtg
     1861 cccatgaagg cgcgtgcctg ttcgtggact tcgtcggtat cgatgaggat ttcgccgata
     1921 tccggctgga aataatcgcg gatggcgcgg atgaccagcg agccttcgag atagatcagg
     1981 aaggcgccgg attgctgctg ggcagcgcct tcgatggcgc tccacagttg cagcaggtag
     2041 ttcaggtccc actgcagctc ttcggcctga cggccgatgg cggcggtgcg ggcgatcagg
     2101 ctcatgcctt gcggcacttc gagctggtcc atgatctcgc gcagttcggc gcgttcgtcg
     2161 ccgtcgacgc ggcgggaaac gccgccgccg cgcgggttgt tcggcatcag cacgaggtag
     2221 cggccggcca gcgagacgta ggtggtcagc gcggcgccct tgttgccgcg ttcgtccttg
     2281 tcgacctgga cgatcagttc ctggccttcc ttgagggcct cgttgatctt ggccttgttg
     2341 gcctcgacac ccggctggaa ataggcgcgg gaaacttcct tgaacggcag gaagccgtgg
     2401 cggtcggcac cgtaatcgac gaagcaggct tcgagcgagg gttcgatgcg ggtgatgacg
     2461 cccttgtaaa tgttgctttt gcgttgttcc ttggcggcgg attcaatgtc gagatcgatc
     2521 agtttctgac catcgacaat ggcgacacgg agttcttccg cctgtgtcgc attgaagagc
     2581 atgcgtttca ttgttttcgg gctccagcgc gccttcggca caccgcagcg aaacgcccat
     2641 gcaggcagcg acaggttcag ttatcgggtt gcgtcatgta ggggaggcaa tggcgatgat
     2701 gaatgtgctg atcgttggat atcgcgagtc tttttatcaa atgcgcgatc tggaattcct
     2761 gccacgggcg atccgtgcgt gcttaaggcg agctaatcca ttaacatcac tacttttggt
     2821 gagtgaactt aaccaggcgt tttacgcgag tttgtcccgg gcaattggca cgggagggac
     2881 ttcgtggcct gccgctcggc agtcgcgcaa aatggcgcgt ggcgggcggt ctgacggttc
     2941 gacatccctt gcaaacagag acgtaaaacg caggcagtat attagaaaat gaatggcgca
     3001 ggtaacaatt cggtcaccca tctcgtgatc ggcgaagagg ctgccggcca gcgcctggac
     3061 aactatctgg tccggcactg caagggggtg ccgaagagtc atttgtatcg catcctgcgc
     3121 agcggcgagg tccgcgtgaa caaggggagg gtagatgcga cctatcgcct gcagcctggt
     3181 gacagcctgc gtttaccccc cttgcgcctt gccgagcggc cggtcgatga gatcgccgag
     3241 cttgccaaga cgcgtgtcga cctgccggtc atctacgagg acgaggcgct gctggtcatc
     3301 gacaagcccg aggggatcgc cgtccatggc ggcagcggcg tcagcttcgg cgtcatcgag
     3361 gccttgcgcc ggcagcggcc gcaggccaag ttcctcgaac tggcacaccg tctcgaccgg
     3421 gagacttccg gcatcctgct ggtcggcaag aagcgcctgg cgctgaccgt gttgcacgac
     3481 atgttccgcg agcacggcgc cggcgccgac aatcgttacc tgg
//
LOCUS       DAOSDX010000204         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9383_length_3518_cov_2.426220, whole genome shotgun sequence.
ACCESSION   DAOSDX010000204 DAOSDX010000000
VERSION     DAOSDX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9383_length_3518_cov_2.426220"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /gene="rpsD"
                     /locus_tag="PLN02_05695"
     CDS             complement(<1..524)
                     /gene="rpsD"
                     /locus_tag="PLN02_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOI51851.1"
                     /translation="MSRYTGPRLKVLRALGVDLPGLSRKSMQERAQPPGQHGARKVTA
                     RKSEFGLQLMEKQKLRYNYGLTERQLRRTVADAKKHRGATGDKLVELLERRLDNLVFR
                     AGFAPTIPAARQLVSHGHFQLNGRRVTIPSIRLRVGDAFGPTAAGSKIDLVRATLEDP
                     ALERPEWIALDSATA"
     gene            complement(545..811)
                     /locus_tag="PLN02_05700"
     CDS             complement(545..811)
                     /locus_tag="PLN02_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51852.1"
                     /translation="MENAEMSLELRLAAVIHLLSSSALRGATFHKTEALRAHLRSVAD
                     SDGLNPYLKSTLQEVLGGWEAVSCHPASIPVDFRSLAEPSCQTH"
     gene            complement(997..1395)
                     /locus_tag="PLN02_05705"
     CDS             complement(997..1395)
                     /locus_tag="PLN02_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HOI51853.1"
                     /translation="MFRIWITASSLLGAIGVAVAAWSTHGLPAFVSADQLAIAIERAR
                     AASLHHMLHTLALFGIALWSRQSPSRWLNVAGALFIFGIAGFAGGIYLIRIAAGIDSG
                     ALIYIVPFGGFCLIFGWLALGIAGWREKPA"
     gene            complement(1417..1764)
                     /locus_tag="PLN02_05710"
     CDS             complement(1417..1764)
                     /locus_tag="PLN02_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HOI51854.1"
                     /translation="MSLKLLHEPETEDPILSVVNLIDIFLVIIAALLITVAKNPLINP
                     FNKQDVTVITDAGKPSMEVLVKKGEKIEKYKASGQIGGGDGEKAGVAYKMKDGSMVYV
                     PETGNATTDDQRR"
     gene            complement(1761..2243)
                     /locus_tag="PLN02_05715"
     CDS             complement(1761..2243)
                     /locus_tag="PLN02_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOI51855.1"
                     /translation="MSNLVELTMYQISQLFLLPTLALIAALFLYAFWVLGEFLVLAWR
                     RRSGHGRPLVAQFRARPTLSDDQLDVLAHKALENPRIASRVTPMLGLVATMIPMGPAL
                     KSLSDGNLAEVSNNLTIAFSAVILALIAASITYWVVNVRRRWLAEEILEIQALRGVAA
                     "
     gene            complement(2277..>3518)
                     /locus_tag="PLN02_05720"
     CDS             complement(2277..>3518)
                     /locus_tag="PLN02_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51856.1"
                     /translation="LLRLRLEGERKFAFGKLSGRVALLDGERRSDTARRWRDALGATT
                     RADEAIRRDENEFSSAVRVDRGIGNGLFSAGLEQSWHRREERQRITGAGALRSDHAAT
                     SKQWSAWLQHEAPLGDKLTLTGGLRGEHIALTADGRAQSASQLAPSLAARFEIAPDLI
                     LRSSIGAGIKAPKIDEISGLTVRATGSNSPLEPDRAGNGSLEAERNLNWEAALEKRLP
                     GNLGIVGANLYLRRTEDFIERRTQREGTRWVERPYNEGTARHWGLELDAKLKTDRLGW
                     KGAALRSHLTLPKARVADRRLGLTRDARELPRYQFTLGVDQALPAWQASAGFHLTQQG
                     RVRTDIPGETSTRQRPKTLLDLYLTRRLTPQLNLRLDAQNLLRADTRRIATFTASADD
                     WRLAGDERGQRTLLLSLEGKW"
BASE COUNT          537 a         1054 c         1277 g          650 t
ORIGIN      
        1 gcggtggcgc tgtcgagggc gatccattcc gggcgttcca gcgccgggtc ttccagcgtg
       61 gcgcggacga ggtcgatctt gctgccggct gcggtcggcc cgaaggcatc gccgacgcgc
      121 aggcggatcg agggaatggt gacgcgtctg ccgttcaact ggaaatggcc gtggctgacc
      181 aactgccggg cggccgggat ggtcggcgcg aagccggcgc ggaagaccag gttgtcgaga
      241 cggcgttcca gcaattcgac cagtttgtcg ccggtggcgc cccgatgctt cttggcatcg
      301 gcgacagtac ggcgcaactg gcgttcggtc agcccgtagt tgtagcgcag cttctgcttt
      361 tccatcagtt gcagaccgaa ctccgatttg cgcgcggtga ccttgcgcgc accgtgctgg
      421 ccgggtggct gggctctttc ctgcatcgat ttgcgtgaca ggccgggcag gtcgacgccg
      481 agggcacgca ggactttgag gcgggggccg gtataacggg acatgaagtt ctctcttgat
      541 attgtcagtg ggtttggcag ctgggttcgg ccagggaacg gaaatcgacg ggaatcgatg
      601 ccgggtggca ggaaaccgct tcccaaccgc cgaggacttc ctgcagcgtg cttttcaggt
      661 aggggttgag gccgtcgctg tcggccacgc tgcgcagatg ggcgcgcagg gcttccgtct
      721 tgtggaaggt ggcgccgcgc agcgcggacg aggacagcag atggatgacg gcagccagcc
      781 tgagttcgag ggacatttcg gcgttttcca tgaagcactc cagtagtttc ggggaaactg
      841 gctggctcgc ccggaatgtg ctagctggct aatgaatgcg aattattcct acttgcaaga
      901 gtgttgtcaa gggtttttta tgccattggg tcacagagcg aatgctgata atccgctttt
      961 cccggcgagg gcggaagatg aggagcgatc ctgtcgttac gccggttttt cccgccagcc
     1021 ggcaatgccc agtgccagcc agccgaaaat caggcagaag ccgccaaagg gaacgatgta
     1081 gatcagcgct ccgctgtcga tgccggcggc gatgcggatc aggtagatgc cgccggcaaa
     1141 gccggcgatg ccgaagatga acaatgcgcc ggcgacgttc agccagcgcg atggcgactg
     1201 gcgtgaccag agggcgatgc cgaacagcgc cagggtgtgc aacatgtgat gcaggctggc
     1261 ggcacgcgcc cgctcgatgg caatcgccag ctggtcggca gacacgaagg cggggagccc
     1321 gtgcgtgctc caggcggcga cggcgacacc gatggcgccg agcagactgc tggcggtgat
     1381 ccagatgcgg aacatgcttt cctcctttgg cggttgtcag cggcgttgat catccgttgt
     1441 ggcgttgccc gtttccggga catagaccat cgagccgtcc ttcatcttgt aggcgacgcc
     1501 ggccttctcg ccgtcaccgc cgccgatttg gccgctcgcc ttgtatttct cgatcttctc
     1561 gccctttttg accagcactt ccatgctcgg cttgccggcg tcggtgatca ccgtcacgtc
     1621 ctgcttgttg aagggattga tcagcgggtt cttggcgacg gtgatgagca gcgcggcaat
     1681 gatcaccagg aagatgtcga tcaggttgac caccgacagg atcgggtcct cggtttccgg
     1741 ctcgtgcagc agcttcaggc tcatgccgcc accccgcgca gcgcctggat ttcgaggatt
     1801 tcctcggcca gccagcggcg ccggacgttg accacccagt aggtgatcga cgccgcgatc
     1861 agcgccagga tcaccgccga gaaggcgatc gtcaggttgt tcgagacctc ggcgaggttg
     1921 ccgtcggaga gcgacttgag cgccggtccc atcgggatca tcgtcgccac taggccgagc
     1981 atcggcgtca cccggctggc gatgcgcgga ttttccagcg ccttgtgcgc gagcacgtcg
     2041 agttggtcgt cggagagcgt cggtcgggcg cggaactggg cgaccagcgg tcgcccgtgg
     2101 ccgctgcggc ggcgccaggc gaggacgagg aattcgccga gcacccagaa ggcgtacagg
     2161 aagagggcgg cgatcagcgc cagcgtcggc agcaggaaga gctgcgagat ctggtacatg
     2221 gtcagttcga caagattgga catggggtct ccgtgcggtg ggtgaatggg ggcggcttac
     2281 cacttgcctt cgagcgacag cagcagcgtg cgctggccgc gctcgtcgcc ggccagccgc
     2341 cagtcgtcgg cgctggcggt gaacgtggcg atgcggcggg tgtcggcgcg cagcaggttc
     2401 tgcgcgtcga ggcgcaggtt cagttgcggc gtcaaccgcc gggtcagata aaggtcgagc
     2461 agggttttcg ggcgttggcg ggtgctcgtc tcgccgggaa tgtcggtgcg cacgcggcct
     2521 tgctgcgtca ggtggaagcc ggcgctggcc tgccacgccg gcaacgcctg gtcgacgccg
     2581 agggtgaact ggtagcgcgg cagttcgcgg gcgtcgcggg tcaggccgag gcgccggtca
     2641 gcgacgcggg ccttgggcag ggtcaggtgg ctgcgcagcg cggcgccttt ccagccgagg
     2701 cggtcggttt tcagcttggc gtcgagttcc agcccccagt ggcgggccgt gccctcgttg
     2761 taggggcgct cgacccagcg cgtgccttcg cgttgcgtgc gccgctcaat gaagtcctcg
     2821 gtgcggcgca ggtaaaggtt ggcgccgacg atgccgaggt tgccgggcag gcgtttttcc
     2881 agcgccgctt cccagttgag gttgcgctcg gcctcgaggc tgccgttgcc ggcgcggtcg
     2941 ggttcgagcg ggctgttgct gccggtggcg cgcacggtca accccgagat ttcgtcgatt
     3001 ttcggcgcct tgatgccggc gccgattgag gagcgcagga tcaggtcggg tgcgatctcg
     3061 aagcgcgccg ccagcgacgg cgccaactgg ctggccgatt gggcacggcc gtcggcggtg
     3121 agcgcgatgt gttcgccgcg caggccgccg gtcagcgtca gtttgtcgcc cagcggcgcc
     3181 tcgtgctgca gccaggcgct ccattgtttc gacgtcgccg catggtcgct gcgcagcgcg
     3241 ccggcaccgg tgatgcgctg cctttcctcg cgccggtgcc agctctgttc gagaccggcg
     3301 gaaaacaggc cgttgccgat gccacggtcg acgcgcacgg cggaggaaaa ttcgttttcg
     3361 tcacggcgga tcgcctcgtc ggcccgcgtt gtcgcgccga gcgcatcgcg ccagcggcgc
     3421 gcggtgtcgc tgcggcgctc gccgtcgagc agtgcgacgc ggccggaaag cttgccgaag
     3481 gcgaacttgc gctcgccttc gagacgcagg cgcagcag
//
LOCUS       DAOSDX010000205         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9388_length_3517_cov_3.419122, whole genome shotgun sequence.
ACCESSION   DAOSDX010000205 DAOSDX010000000
VERSION     DAOSDX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9388_length_3517_cov_3.419122"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLN02_05725"
     CDS             complement(<1..450)
                     /locus_tag="PLN02_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3334 family protein"
                     /protein_id="HOI51857.1"
                     /translation="MNVKISPVVFGTEDILRSLCNSVADVLNVATCSQVRYSGMVQRI
                     TKTCLKPDIGCFVLFDGGFTGLVVLNFSAAAAMEIYEGYLLSMGMTRDDLATSHTADE
                     VSNVMGELMNQIVGNFTGKIGRELQTHITQNQPKMLVLNKQVILSVDT"
     gene            546..863
                     /locus_tag="PLN02_05730"
     CDS             546..863
                     /locus_tag="PLN02_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51858.1"
                     /translation="MRHIVIELHTDAPAKPAAGEPCTGCGVCCALETCPLGRLRFLQR
                     RGPCPALRWSADEMRYHCSLLRAPAEHFPALPAAFAPLASRLAARWISAGSGCDCDAD
                     VQS"
     gene            complement(854..1216)
                     /locus_tag="PLN02_05735"
     CDS             complement(854..1216)
                     /locus_tag="PLN02_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOI51859.1"
                     /translation="MSTTLIQRHGTCQRYSDSVVHAGTVYLVEIAADTTLDAAGQAAS
                     MLAGVDRLLAQAGSDRRHLLMATLYLADMADYDAVNAIWDAWLPAGCAPTRACVQARL
                     AKPEYRVEIALTAAVAQD"
     gene            1524..2111
                     /locus_tag="PLN02_05740"
     CDS             1524..2111
                     /locus_tag="PLN02_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51860.1"
                     /translation="MDNLIKDTATLELFCWLAPEAELPGWDSLRGSPFGASLSAPEGG
                     APSAGDQLLSVQNYFAEYHLEYFRQRRYSHFPSRLHALLQFATRTDAMTYRFKHPDRV
                     FGKNLSCSRTKGAYICSFHDASWLDYLRLPHSLSLAALDEVADHYWSGRTVEEIGLTF
                     MDEPWQEPPVIEALFQGALEPVWGHQQSGWLPGFR"
     gene            complement(2104..3435)
                     /locus_tag="PLN02_05745"
     CDS             complement(2104..3435)
                     /locus_tag="PLN02_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51861.1"
                     /translation="MRWLPRSLFRQNLLLLVALILLGQLISALVFIFAVQQPRAVRLA
                     GLAAQQVQAIQQAAARLEDGPRQAFLAAMNVGDEFRVLPAAALPLDRTEFAGFDEPDS
                     VAVRGFLRRLREALGERAQIVRWQASDRSIWVGILIGDDPYWVTLRGGQLLPVAPWLF
                     LGLSAAATLLAIFGAVGISRRINRPLARLVEAAQVMGKGERPPRLADDGPQEIATVAA
                     SFNQLADDLAALDRERAVMLAGVSHDLRTPLTKLRLALDISEARLEPALFAQMGRHID
                     EIDGLLDQFLAFARIGDDEEPRRCAIDPLVGAVAEAFIADGMALHLQAGADVSLLLRE
                     KAIRRLLVNLLDNARKYGGGEVSLVTRVDGGWCRIEVCDDGPGIAPQDVERLLQPFVR
                     ADAARAGKPGTGLGLAIVRRIVDLHGGKLDLTPRGDGKPGLRVSVALPLAT"
BASE COUNT          596 a         1214 c         1157 g          550 t
ORIGIN      
        1 ggtatcgacg ctgaggatca cctgcttgtt gaggaccagc atcttcggct ggttctgggt
       61 gatgtgggtc tgcagttcgc gaccgatctt gccggtgaaa ttgccgacga tctggttcat
      121 cagctcgccc atcacgttgc tcacctcgtc cgcggtatgc gaggtggcca ggtcgtcacg
      181 cgtcattccc atgctgagca ggtagccctc ataaatctcc atcgctgccg cggccgagaa
      241 attgagcacg accaacccgg tgaaaccgcc gtcgaacagc acgaagcagc cgatatcggg
      301 tttcaggcag gtcttggtga tgcgctggac catgccggaa tagcgcacct ggctgcaggt
      361 agcgacattg agcacgtcgg ccaccgaatt gcacagactg cgcaggatgt cttcggtgcc
      421 gaagacgaca ggcgagattt ttacgttcat gatcgggcaa ggctgtggaa tgaagtgtcg
      481 actatagcgc agcgccgccc gggccgggct tgcgcggctt tcgcgggacg gcgcatgatc
      541 cgcccatgcg ccacatcgtc atcgaactac acaccgacgc cccggccaag ccggcggcgg
      601 gcgaaccgtg caccggctgc ggcgtctgct gcgcgctcga gacctgcccg ctcggccgcc
      661 tgcgcttcct gcagcgacgc ggcccctgcc cggcgctgcg ctggtcagcg gacgagatgc
      721 gctaccactg cagcctgcta cgcgcgccgg ccgagcattt cccagccctg cccgccgcct
      781 ttgcaccgct cgcttcccgc ctcgccgccc gctggatttc agccggcagc ggctgcgact
      841 gcgacgccga cgttcagtcc tgagcgacag ccgcagtcag tgcgatctcg acccggtatt
      901 ccggcttcgc cagtcgcgcc tgcacacagg ccctggtcgg cgcgcagccg gccggcagcc
      961 aggcgtccca gatcgcgttg accgcgtcgt aatcggccat gtcggcgagg tagagggtgg
     1021 ccatcaacaa gtggcggcgg tcgctgccgg cctgcgccag caggcggtcg acgccggcca
     1081 gcatggaagc ggcctgaccg gcggcgtcaa gcgtggtatc ggcggcaatt tcgaccagat
     1141 agacggtgcc ggcatggacg acgctgtcgg aataacgctg gcaagtgccg tggcgctgaa
     1201 tgagagtagt ggacaaagcg ttccccaaaa gcaggaagcc cacgcgcgac tggcatgggc
     1261 ttcggtttac agcaaccccg gcacggcggc tggggcaatc ggcgttacgc cgaatctgga
     1321 gtactacacg gcaatctcaa gaaaaacttc ctgtgacaac gtccatagaa tctcctgcaa
     1381 cgcagcttcg actgccactg catgcccggc ttgctgcaca cctgacacga gcccagatta
     1441 ccacttcggc ggaaaaaacc aagtagttat tttcgttgcg tgatcggtca taatcatgcg
     1501 attcgctgcc gcccgaccgg gacatggaca acctgatcaa ggacaccgcc acgctggaac
     1561 tcttctgctg gctcgccccc gaggccgagt tgccgggctg ggacagcctg cgcggcagcc
     1621 cgttcggtgc ctccctgtcg gcgccggaag gcggcgcgcc gagcgctggc gaccagttgc
     1681 tgtcggtgca gaactacttc gccgaatacc atctcgagta tttccgccag cgccgctaca
     1741 gccatttccc cagccgcctg cacgcgctgc tgcagttcgc cacgcgcacc gacgcgatga
     1801 cttaccgctt caagcacccc gaccgcgtct tcggcaagaa cctgtcgtgc tcgcggacca
     1861 agggcgccta catctgttcc ttccacgacg cgagctggct cgattacctg cgcctgccgc
     1921 acagtctctc gctcgccgca ctcgacgaag tcgccgacca ctactggtcg gggcgcactg
     1981 tcgaggaaat cggcctcacc ttcatggacg agccctggca ggagccgccg gtcatcgaag
     2041 cgctgttcca gggggcgctg gagccggtct ggggccacca gcagtcgggc tggctgcccg
     2101 gtttcaggta gccagcggca gagcgacgct gacccgcagg ccgggcttgc cgtcgccgcg
     2161 tggcgtcaga tcgagctttc ccccgtgcag atcgacgata cgccggacaa tggccaggcc
     2221 gagaccggtg cccggcttgc cggcacgcgc cgcgtcggcg cgcacgaaag gctgcagcag
     2281 gcgctcgaca tcctgcgggg cgatgcccgg cccgtcgtcg cagacctcga tgcggcacca
     2341 gccgccgtcg acgcgtgtga cgagtgaaac ctcgccgccc ccgtacttgc gtgcgttgtc
     2401 gagcaggttg accagcagcc ggcggatcgc cttttcgcgg agcagcaggc tgacgtcggc
     2461 gcctgcctga aggtgcagcg ccatgccgtc ggcgatgaag gcctccgcca ccgcgccgac
     2521 cagcgggtcg atggcgcagc ggcgcggctc ctcgtcgtcg ccgatgcggg cgaaggcgag
     2581 gaactggtcg agcaggccgt cgatctcgtc gatgtggcgc cccatctgcg cgaacagcgc
     2641 cggctccagc ctggcctccg aaatatccag cgccaggcgc agcttggtca gcggcgtgcg
     2701 caggtcgtgc gagacgccgg ccagcatcac cgcccgctcg cggtcgaggg cggcgagatc
     2761 gtcggcgagc tggttgaaac tggcggcgac ggtggcgatt tcctgcggcc cgtcgtcggc
     2821 caggcgcggt ggtcgctcgc ccttgcccat gacctgcgcc gcttcgacca ggcgggcgag
     2881 cggccggttg atgcggcgcg agatgccgac cgcgccgaaa atcgccagca gcgtcgccgc
     2941 cgccgacaag ccgaggaaca gccagggcgc caccggcagc aactggccgc cgcgcagggt
     3001 cacccagtag gggtcgtcac cgatcaggat gccgacccag atgctgcggt cgctcgcctg
     3061 ccaacggaca atctgcgccc gctcgccgag cgcttcgcgc aggcggcgca ggaagccgcg
     3121 cacggcgacc gagtccggct catcgaaacc ggcgaattca gtgcggtcga gcggcaacgc
     3181 tgcggccggc agaacgcgga attcgtcgcc gacgttcatc gccgccagga aggcctggcg
     3241 cggcccgtct tccagtcgcg ccgccgcctg ctgtatcgcc tgcacctgtt gcgcggccag
     3301 cccggcaagg cggacggcgc gcggctgctg cacggcgaag atgaaaacca gcgcgctgat
     3361 caactgcccg agcaggatca gggcgaccag cagcagcagg ttctgacgga acagcgagcg
     3421 cggcagccag cgcatctcag tccgccgccc cgggatcggc aacgaagacg tagccgacgc
     3481 cccacaccgt gcggatatgg cgcggctgcg ccgggtc
//
LOCUS       DAOSDX010000206         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9399_length_3514_cov_3.259324, whole genome shotgun sequence.
ACCESSION   DAOSDX010000206 DAOSDX010000000
VERSION     DAOSDX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9399_length_3514_cov_3.259324"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLN02_05750"
     CDS             <1..909
                     /locus_tag="PLN02_05750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOI51862.1"
                     /translation="VFLVCAGFLAARGLAPEGRLLAGNPLPLIGRRYLRLALPFVAAI
                     SIAVIVSAIADQWLDDPAVPDRATFGQWLAHVFLLHGVLGAESLSAGVWYVAIDFQLY
                     AVMALLLWLGRKPSGALLAVLGLAIASLFHFNRDSSLDNWAIYFFGSYGLGAAAWWAS
                     ARGRLRLWLGLIATIGIAALVEDFRLRIAIALAVALLLAFSRRSGLLERWPQGRLAAF
                     FGRISYSVFLVHFPVLLLANALFANSAPRDAAAITWWTLVAWGACIGAGVAFHRWIEV
                     PAGKVQMPALLRQWGRRALQALPGRG"
     gene            complement(906..2864)
                     /locus_tag="PLN02_05755"
     CDS             complement(906..2864)
                     /locus_tag="PLN02_05755"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOI51863.1"
                     /translation="MTYPIAAAPLWTPNPATVGDTRMAQFMQATGHGRYAELWQWSVD
                     RPEEFWPQVWRFCAAVGERGETVLEDGGKMPGARWFPQARLNYAENLLRHADDGEALL
                     FWGEDKVKRRMTRRELRGEVARFQRFLIEAGVGEGDRVAGYLPNLPETLAAMLATTAL
                     GAIWSSASPDFGVQGVLDRFGQIEPKVLVCVDGYWYNGKAVDCLAKNAEVAAQMPSLV
                     KTVVVPYLSQAPEIGSIANAVAWPALPVADAATMPVFKRVAFDHPLFIMYSSGTTGVP
                     KCIVHCHGGVLLQHLKEHVLHSDVRPCDRLFYFTTCGWMMWNWLVSGLAAGATLLLYD
                     GSPFAAGGKVLFDYAEAERMTHFGTSAKFIDAAAKQGLTPARTHDLGALRALFSTGSP
                     LSPEGFDWVYRELKADILLASISGGTDIISCFVLGNPVLPVYRGEIQCRGLGMAVEVF
                     DEDGRPVRGEKGELVCTRPFPVMPVGFWNDADGSKYRAAYFERFDNIWCHGDFSELTA
                     HDGMIIYGRSDATLNPGGVRIGTAEIYRQVEQLPEVLESLVIGQDWPPGRNDDVRVVL
                     FVKLAEGAVLDAALVERIKQQIRANTTPRHVPAKIVQVPDIPRTKSGKIVELAVRNVV
                     HERPVKNVEALANPEALEGFRQRPELAD"
     gene            2980..>3514
                     /locus_tag="PLN02_05760"
     CDS             2980..>3514
                     /locus_tag="PLN02_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOI51864.1"
                     /translation="MSNVVLSEIHGKVGLIRINRPEAMNALNNDVVDGIAAAIDAFEA
                     DENIGCIVLTGNEKAFAAGADIGFMKDFDYMHAYQTDFITRNWERIKTTRKPVIAAVA
                     GFALGGGCEMAMMCDMIFAADTAKFGQPEIRLGTLPGAGGTQRLPRAVGKAKAMDLCL
                     TARMMDAAEATKAGLVAR"
BASE COUNT          548 a         1227 c         1151 g          588 t
ORIGIN      
        1 gtcttcctcg tctgcgccgg tttcctcgct gcccgcggcc tggcaccgga aggccgcctg
       61 ctggcgggca acccgctgcc gctgatcggc cgccgctacc tgcgcctcgc cctgcccttc
      121 gtcgcggcga tcagcatcgc cgtcatcgtc tcggccatcg ccgaccagtg gctcgacgat
      181 ccggcggtgc ccgaccgcgc caccttcggc cagtggctgg cccatgtctt cctgctgcac
      241 ggcgtgctcg gcgccgaatc actgtccgcc ggcgtctggt acgtcgccat cgacttccag
      301 ctctacgccg tgatggcgct gctgctgtgg ctggggcgca agccgtctgg cgcgctactg
      361 gcggtgctcg ggctggcgat tgcctcgctg tttcatttca accgcgacag cagcctcgac
      421 aactgggcga tctacttctt cggttcctac ggcctcggcg ccgcggcctg gtgggcctcg
      481 gcgcgcggcc gcctgcgact ctggctggga ctcatcgcca ccatcggcat cgcggcgctg
      541 gtcgaggact tccgcctgcg catcgcgatt gccctggcgg tggcgctgct gctcgccttc
      601 agccggcgca gcggcctcct cgaacgctgg ccgcaggggc ggctggcggc cttcttcggg
      661 cgcatttcct actcggtctt cctcgttcac tttcccgtcc tgctcctcgc caacgcgctg
      721 tttgccaaca gcgccccgcg cgatgcggcg gcgatcacct ggtggacgct agtcgcctgg
      781 ggcgcctgca tcggcgccgg cgtggccttc catcgctgga tcgaagtccc ggccggcaag
      841 gtgcagatgc cggccctgct ccgccagtgg ggccgccgcg ccttgcaggc cctgcccgga
      901 cgcggttaat cggccagttc ggggcgctgc cggaaacctt ccagcgcctc cggattggcc
      961 agcgcctcga cgttcttcac cggccgctcg tgcaccacgt tgcgcaccgc cagttcgacg
     1021 atcttgcccg acttggtacg cggaatgtcc ggcacctgga cgatcttcgc cggcacgtgg
     1081 cgcggcgtcg tgttggcgcg gatctgctgc ttgatgcgct cgaccagcgc ggcgtcgagc
     1141 acggcgccct cggccagctt gacgaagagc accacgcgca cgtcgtcgtt gcggcccggc
     1201 ggccagtcct ggccgatcac caatgactcc agcacttccg gcaactgctc gacctggcgg
     1261 tagatttcgg cggtgccgat acgcacgccg ccggggttga gcgtcgcgtc ggagcggccg
     1321 tagatgatca tgccgtcgtg cgcggtcagc tccgaaaaat cgccgtggca ccagatgttg
     1381 tcgaagcgtt cgaagtaagc ggcgcggtac ttgctcccgt cggcatcgtt ccagaagcca
     1441 accggcatca ccgggaaggg ccgggtgcac accagttcgc ccttctcgcc gcgtaccggc
     1501 cggccgtcct cgtcgaacac ctcgaccgcc atgcccagac cgcggcactg aatttcgccg
     1561 cgatagactg gcagcaccgg gttgccgagg acgaagcagg aaatgatgtc ggtgccgccg
     1621 gaaatcgagg cgagcaggat gtcggccttg agctcgcgat agacccagtc gaaaccttcc
     1681 ggcgacagcg ggctgccggt cgagaacaag gcgcgcaggg cgcccaggtc gtgcgtgcgg
     1741 gccggcgtca ggccctgctt ggcggcggcg tcgatgaact tggccgaggt gccgaagtgg
     1801 gtcatgcgct cggcttcggc gtagtcgaac agcaccttgc cgccggccgc gaacggcgag
     1861 ccgtcgtaga gcagcagcgt ggcgccggcg gccagcccgg agaccagcca gttccacatc
     1921 atccagccgc aggtggtgaa gtagaacaag cggtcgcagg ggcgcacgtc gctgtgcagg
     1981 acatgctcct tgaggtgttg cagcagcacg ccgccgtggc aatggacgat gcacttgggc
     2041 acgccggtcg tgcccgacga gtacatgatg aacagcggat ggtcgaaggc gacccgtttg
     2101 aacaccggca tcgtcgccgc atcggccacc ggcagcgccg gccaggcgac ggcgttggcg
     2161 atactgccga tttccggggc ttggctcaga tacggcacga ccacggtctt caccagcgac
     2221 ggcatctgcg ccgccacttc cgcgttcttg gccagacaat cgaccgcctt gccgttgtac
     2281 cagtagccat cgacgcagac cagcaccttg ggttcgatct ggccgaagcg atcgagcacg
     2341 ccctgcacgc cgaaatccgg cgaggccgac gaccagatgg cgccgagcgc cgtcgtcgcc
     2401 agcatggcgg cgagcgtttc cggcaggttg ggcaggtaac cggcgacgcg gtcgccttcg
     2461 ccgaccccgg cctcgatcag gaaacgctgg aagcgggcga cttcgccgcg caactcgcgg
     2521 cgagtcatac gccgcttgac cttgtcctcg ccccagaaca gcagcgcttc gccgtcatcg
     2581 gcgtggcgca gcaggttttc ggcgtagttc aggcgagcct gcggaaacca gcgcgcgccg
     2641 ggcatcttgc cgccatcctc gagcaccgtc tcgccgcgct cgccgaccgc agcgcaaaag
     2701 cgccagacct gtggccagaa ttcctcaggc cggtcgaccg accattgcca cagttcggcg
     2761 tagcggccgt ggccggtcgc ctgcatgaac tgcgccatgc gggtgtcgcc gacggtggcc
     2821 gggttcgggg tccacaacgg ggccgcggct atcggatagg tcatcttgtc tcctccggga
     2881 tggtttttcg tgacagctta tcaggttaac gttaacgtca actgtcgcgc gccggacaat
     2941 gcggcgccag ccttcaacaa gaacatccag gagaatccaa tgagcaacgt cgtactgagc
     3001 gaaatccacg gcaaggtcgg tctgatccgc atcaaccggc cggaagcgat gaacgcgctg
     3061 aacaacgatg tcgtcgacgg catcgccgcc gccatcgacg ccttcgaagc cgacgagaac
     3121 atcggctgca tcgtcctcac cggcaacgaa aaagccttcg ccgccggcgc cgacatcggc
     3181 ttcatgaagg acttcgacta catgcacgcc taccagaccg acttcatcac ccgcaactgg
     3241 gagcgcatca agaccacgcg caagccggtg atcgcggcgg tggccggctt cgcgctgggc
     3301 ggcggttgcg agatggcgat gatgtgcgac atgatcttcg ccgccgatac ggcgaagttc
     3361 ggccagccgg aaatccgcct cggcaccctg cccggcgccg gcggcacgca gcgcctgccg
     3421 cgcgccgtcg gcaaggcgaa ggcgatggac ctgtgcctga ccgcgcgcat gatggacgcc
     3481 gccgaagcca caaaggccgg tctggtcgcc cgtg
//
LOCUS       DAOSDX010000207         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9418_length_3507_cov_3.280417, whole genome shotgun sequence.
ACCESSION   DAOSDX010000207 DAOSDX010000000
VERSION     DAOSDX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9418_length_3507_cov_3.280417"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLN02_05765"
     CDS             <1..740
                     /locus_tag="PLN02_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOI51865.1"
                     /translation="TRTTTHVRFGVDGGIGTAGMQALLAGLGYRTPRTVSVAGFADPT
                     VGALWRFHKSPVDSAILGFGVRFGIARADDPNNLFDIPPGDGSTDLRSRLEYFRDLGQ
                     GFDLHLLAEYQVQLPDHVRARPGNPLTTATVERLRRNLGDYWESDVEIGKRFGNWRLA
                     ATWHRYQEKPDRYRSPSGADTSALSAHTDTRADQLRLALSWSGIEAWQGGHLPMPLVV
                     KLEVQDAVRGQNFVAVRDVYLRLTSAF"
     gene            745..2571
                     /locus_tag="PLN02_05770"
     CDS             745..2571
                     /locus_tag="PLN02_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51866.1"
                     /translation="MTRRFLLLLLFVWLAGAARAGEAPLIDFSEADFVLADSATPPPD
                     SAPWQTVTLPDQWRLTRPGVAGNGWYRLRFRIDRPDPGLQAVYLPKLVMNAAVFLNGE
                     AIGDGGSFSEPIARNWNRPLFFPVPGELLRPGDNVLHVRLLGHPYTQAALHPLQLGPE
                     NQLRPIYERAYFLNITLNQTAGLLIAAVGVLTLNLWLRRRQETAYGYFGLSALVWSLQ
                     ATNLYLNQAPVDTAHWEILTNAGFQIFSGFLLISLLRFVGLRQPALVGTLWFGIFAAP
                     LSLSLAPAAWYLPLTAGWHFYTLLAALSTLALLLRAAWRERNRDARFLVGAMGLVVLF
                     GLHDWLIHSQHIWQGRTAWPLTDVFLLHYSAPLVFLALGLIMTGRYVSALNEYEALAD
                     ELDHRVQDKHLQLQESYARMRELETERAVTEERERIFRDLHDDVGAKLLSLVYRAQRA
                     EDADLARSALRDLRDVVSYTGAAHFTLDELAADWRSECEQRLSEAGIRLDWQSDGAAE
                     ALSQPQALNIGRILREAISNVIRHAGASSVAIRLEASEDALHLSIADDGRGLAEDALP
                     RGRGLRNMQVRAQRLGGEVVQVNGDGGGCRIALRVPLTAPVA"
     gene            complement(2584..>3507)
                     /locus_tag="PLN02_05775"
     CDS             complement(2584..>3507)
                     /locus_tag="PLN02_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HOI51867.1"
                     /translation="FNRSLALWQQALNAISQRFGLNLWGAHHRTLIDSGVSGERGLGF
                     WATGDYAHERDRDGRQVLGEFGVYHDLTPNLRWGIGVGRNDYRQDQAFGGRSKADGSY
                     LVLEADYAPGTRRDWGYSVTGLYGRMDTKLRRGYLNAGVADASTGTPDATSWALRLRV
                     DRYDAWRTGEVSWSPFVAYTHVSSKLDSYIETGGGFPVAYGSQHRRSDELRGGLVGRL
                     ALGANTDLRASGELVVARNRSGGSSGLLLGAGGFAFNTGSSRDTETWGRLGLEIDHRL
                     TAKAALATSLNVASDGNDPQWSGSVGVRVAF"
BASE COUNT          527 a         1352 c         1102 g          526 t
ORIGIN      
        1 atacccggac gacgacccac gtccgcttcg gcgtcgatgg cggcatcggc accgccggca
       61 tgcaggcgct gctcgccggc ctcggctacc ggacgccgcg cacggtcagc gtcgccggtt
      121 tcgccgaccc gaccgtcggc gccctctggc gtttccacaa gagcccggta gacagcgcca
      181 tcctcggttt tggcgtgcgc ttcggcatcg cccgcgccga cgacccgaac aacctcttcg
      241 acatcccgcc cggcgacggc agcaccgacc tgcgcagccg cctcgaatac ttccgcgacc
      301 tcggccaggg cttcgacctg cacctgctcg ccgaatacca ggtgcaactg cccgaccacg
      361 tccgcgcccg ccccggcaac ccgctgacca ccgccacggt cgagcgcctg cgccgcaacc
      421 tcggcgacta ctgggaatcg gacgtcgaga tcggcaagcg tttcggcaac tggcggctgg
      481 cggcgacctg gcaccgctac caggaaaagc ccgaccgcta ccgctcgccg tccggcgccg
      541 acaccagcgc gctgtcggcg cacaccgaca cccgcgccga ccagttgcgg ctggcgctca
      601 gctggagcgg catcgaagcc tggcaaggcg gccacctgcc gatgccgctg gtggtcaagc
      661 tggaagtcca ggacgccgtg cgcggccaga acttcgtcgc cgtgcgcgat gtttacctgc
      721 ggctgaccag cgccttctga ccgcgtgacg cgccgcttcc tgctcctcct gctgtttgtc
      781 tggctggccg gcgccgcccg ggccggcgag gcgccgctca tcgacttcag cgaggccgac
      841 ttcgtcctcg ccgacagcgc gacgccgccg cccgacagcg ccccctggca gacggtgacg
      901 ctgcccgacc agtggcgcct gacgcgcccc ggcgtcgccg gcaacggctg gtaccggctg
      961 cgctttcgca tcgaccggcc ggaccccggc ctgcaggcgg tctacctgcc gaagctggtg
     1021 atgaacgccg ccgtcttcct caacggcgaa gcgatcggcg acggcggcag cttcagcgaa
     1081 ccgatcgccc gcaactggaa ccggccgctg ttcttcccgg ttcccggcga actgctgcgc
     1141 cccggcgaca acgtcctgca cgtccggctg ctcggccacc cctacacgca ggcggcgctg
     1201 cacccgctgc agctcggccc ggaaaaccag ctccgcccga tctacgaacg cgcctatttc
     1261 ctcaacatca cgctcaacca gaccgccggc ctgctgatcg ccgccgtcgg cgttctcacc
     1321 ctcaacctct ggctgcgccg ccgccaggaa accgcttacg gctatttcgg cctgtcggcg
     1381 ctggtctggt cgctgcaggc gaccaacctc tacctcaacc aggcgccggt cgataccgcg
     1441 cactgggaaa tcctgaccaa cgccggcttc cagatcttct ccggctttct gctcatctcg
     1501 ctgctgcgtt tcgtcggcct gcgccagccg gcgctggtcg gcacgctgtg gttcggcatc
     1561 ttcgccgcgc cgctcagcct gtcgctggcg cccgccgcct ggtacctgcc gctgaccgcc
     1621 ggctggcact tctacaccct gctcgccgcg ttgagcacgc tcgccctgct gctgcgcgcc
     1681 gcctggcgcg agcgcaaccg cgacgcccgt ttcctggtcg gcgcgatggg gctggtcgtc
     1741 cttttcggcc tgcacgactg gctgatccac agccagcaca tctggcaggg gcgcaccgcc
     1801 tggccgctga ccgacgtctt cctgctgcat tacagcgcgc cgctcgtctt cctcgccctc
     1861 ggcctcatca tgaccggccg ctacgtcagc gcgctcaacg aatacgaggc gctggccgac
     1921 gaactcgacc accgcgtgca ggacaagcac ctgcagttgc aggagagtta cgcgcggatg
     1981 cgcgaactgg aaaccgaaag ggcggtgacc gaggagcgcg aacgcatctt ccgcgacctg
     2041 cacgacgacg tcggcgccaa gctgctgtcg ctggtctatc gcgcccagcg cgccgaggac
     2101 gccgacctgg cccgctcggc gttgcgcgac ctgcgcgacg tcgtctccta caccggcgcc
     2161 gcccacttca cgctcgacga gctggccgcc gactggcgct cggaatgcga gcagcgcctg
     2221 agcgaggccg gcatccgtct cgactggcag agcgacggcg ccgccgaagc cctgagccag
     2281 ccgcaggcgc tgaacatcgg ccgcatcctg cgcgaggcga tttccaacgt gatccgccat
     2341 gccggcgcca gcagcgtcgc catccgcctc gaagccagcg aggacgccct gcacctgagc
     2401 atcgccgacg acggccgcgg cctggccgaa gacgccctgc cgcgcggccg cggtctgcgc
     2461 aacatgcagg tgcgcgccca gcgactgggc ggcgaagtgg tgcaggtcaa cggcgacggg
     2521 ggcggttgcc ggatcgccct gcgcgtcccg ctcaccgccc ccgtcgcttg attcccggcg
     2581 cgtttagaaa gcgacgcgca ccccgacgct gcccgaccac tgcggatcgt tgccgtccga
     2641 cgcgacattg agcgaagtcg ccagcgccgc ctttgcggtc aaccgatggt cgatctccag
     2701 cccgagccgg ccccaggtct cggtatcgcg cgaactgccg gtgttgaagg caaagccgcc
     2761 ggcacccagc aacaggccgc tgctaccgcc gctgcggttg cgcgcaacga ccagttcgcc
     2821 gctcgcccgc aggtcggtgt tggcgcccag cgccagacgg ccgaccagac cgccgcgcaa
     2881 ctcgtcgctg cgccggtgct ggctgccgta ggcgaccggg aaaccgccac cggtttcgat
     2941 atagctgtcg agcttgctgc tgacgtgcgt gtaggcaacg aacgggctcc aggaaacctc
     3001 gccggtccgc caggcatcgt agcgatcgac ccgcaggcgc agcgcccagc tggttgcatc
     3061 gggcgtaccg gtcgacgcat cggcgacgcc ggcattcagg taaccgcggc gcagcttcgt
     3121 atccatccgg ccatacaacc cggtcacgct gtacccccag tcacgccggg ttccgggcgc
     3181 gtaatcggcc tcgagcacca gataactgcc gtcggccttg ctacgtccgc caaaggcctg
     3241 atcctgccgg tagtcgttgc ggccgacgcc gatgccccag cgcaggttcg gcgtcagatc
     3301 atgatagacg ccgaattcgc cgagcacctg gcgaccgtcg cggtcccgct cgtgggcgta
     3361 gtcgccggtc gcccagaaac cgaggccgcg ctcgccgctg acgccggaat cgatcagcgt
     3421 ccggtggtgc gcgccccaca ggttgaggcc gaagcgctgc gaaatggcgt tcaacgcctg
     3481 ctgccacagc gccagcgaac gattgaa
//
LOCUS       DAOSDX010000208         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9455_length_3499_cov_3.612660, whole genome shotgun sequence.
ACCESSION   DAOSDX010000208 DAOSDX010000000
VERSION     DAOSDX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9455_length_3499_cov_3.612660"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLN02_05780"
     CDS             complement(<1..544)
                     /locus_tag="PLN02_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HOI51868.1"
                     /translation="MLSSLPVLGADPAVADPAADYQLAQQHFVGFGVARDDRRGLFYL
                     KRAAEGGHAEAQFKLGNYLHMYAYDIKGAAQWWQRAAAQGHQGAASNLAEAQAAGWIA
                     PEPGAAPKARPPVAAPVAEAAPAGDLPDIARALTEDAGWRALGDEAASLQVFASSSEA
                     EVRALAARHAWKRPLALLAFE"
     gene            749..2059
                     /locus_tag="PLN02_05785"
     CDS             749..2059
                     /locus_tag="PLN02_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HOI51869.1"
                     /translation="MHPRLILAELEEHPLRHRLNNEFHARPATALAGAVLISHLVFKH
                     SATTVAAERENLSKLCQGHVCNAIDSSESHLMIETGAFRMRWELHTEFSTYTFFRDLN
                     ADEALPVDATAFDAVHPEWLSGIPGKLIVATQVEVRPASELSPDAVLAGLTPSGRTLG
                     GARVADDAAWVFTDFKIDNGFSRFLVINENMTQRQTGRTVQRLVEIETYRMMALLGLP
                     IAKEVGAWLYGGEKRLAELMERIGQAKTPEDERDVLASLSALAAEVENSVARTTFRFG
                     ASRAYHGLVSQRIEELRETRIPGLPTLDEFMQRRLLPAMKTCEAISRRQEELSGRVAR
                     NSQLLRTRVDIELERQNQELLTQMNRRAKLQLRLQETVEGLSVVVLTYYGSQLVQYLA
                     KGTKEVHHLNTDVITAVSIPLIAGLVAWGTRRMRRKLAAEEAAG"
     gene            complement(2067..>3499)
                     /locus_tag="PLN02_05790"
     CDS             complement(2067..>3499)
                     /locus_tag="PLN02_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI51870.1"
                     /translation="FGIDLGPIIKARNSLAGQGMLIAFGELLLSAGLLMLIGFLMTRQ
                     LGALMAASRAVTEGNLTPARVPEGRDDLGQLGAAFNVMSQTVADRVGQLVAAREAAEA
                     ANVAKSRFLATMSHEIRTPLNGILGMAQLLQSPTMSDAERLDYARVIMESGETLLVLL
                     NDILDLSKVEAGRMLLQRQSFLPGMLVKDSLRLYAENAASRGLTLRGQWPEGEPPACL
                     GDPVRVRQMLSNLIHNALKFTERGGVEVIASFRQAGNQVRLHIEVCDTGIGIPSEAQA
                     RLFTPFTQVDTSSTRAYGGSGLGLSIVSRLAHLMDGEVGIDSTPGVGTRAWFEIVLEA
                     SAEALPIAAECGISAADRLPARVLVVEDNAVNRRIVEAMLDKLGVAHDSVGDGAAAVA
                     AVQAAPHAYETVLMDCQMPVLDGFAATRQIRSWERDNERRPVPVIALTASVFAEDRAQ
                     CEAAGMTGFLAKPVVFEQLRAALAAD"
BASE COUNT          600 a         1220 c         1160 g          519 t
ORIGIN      
        1 gctcgaaggc aagcagcgcg agcggccgtt tccaggcgtg gcgcgccgcc agcgcgcgca
       61 cctcggcctc gctgctcgac gcgaacacct gcaggctcgc cgcctcgtcg cccagcgcgc
      121 gccagccggc atcctcggtc agcgcccggg cgatgtcggg caggtcgccg gccggcgccg
      181 cttcggccac cggggcagca actggcggtc tggccttggg cgctgcgccc ggctcgggcg
      241 cgatccagcc ggcggcctgc gcctcggcca ggttgctggc ggctccctgg tggccctgcg
      301 ccgcggcccg ctgccaccac tgcgccgcgc ccttgatatc gtaggcgtac atgtgcaggt
      361 agttgccgag cttgaactgg gcttcggcgt ggccgccttc cgccgcgcgc ttgaggtaga
      421 acaacccccg ccggtcgtcg cgggcgacgc cgaaaccgac gaaatgttgc tgcgcgagct
      481 ggtagtcggc agccggatcg gcgacggcgg ggtcggcgcc gagcaccggc agagaggaaa
      541 gcatcagaag gcaggcggcg aaagccaggg aagaacgcat gcgaaaaagc ggcgcgaagc
      601 cggaatgctg aaacacgcga cattgtaggg cgtgttcatc gctagctgct gaagacagtt
      661 cgacgcctct ccctgcgggc ttcgccgtga cgagcaggac gcggtgctac aatcttttgc
      721 accgcagcaa tcgcctccgg ataccgccat gcaccctcgc ctgattctcg ccgaactgga
      781 agaacaccca ctccgtcacc ggctcaacaa tgaattccac gcccgcccgg cgaccgcgct
      841 ggccggcgcc gtgctgatct cgcacctggt cttcaagcac agcgcgacga ccgtcgccgc
      901 cgaacgcgaa aacctgtcga aactttgcca ggggcacgtc tgcaacgcca tcgacagctc
      961 ggaatcacac ctgatgatcg agaccggcgc cttccgcatg cgctgggagc tgcataccga
     1021 gttctcgacc tacaccttct tccgcgacct gaacgccgac gaggcactgc cggtggacgc
     1081 caccgccttc gacgcggtgc atccggaatg gttgtccggc attcccggca aactgatcgt
     1141 cgccacccag gtcgaagtcc ggccggccag cgagctgtcg cccgacgcgg tgctcgccgg
     1201 cctgacgccg agcgggcgca cgctcggcgg cgcccgcgtc gccgacgacg cggcctgggt
     1261 gttcaccgac ttcaagatcg acaacggctt ctcgcgtttc ctggtgatca acgagaacat
     1321 gacgcagcgc cagaccgggc gcaccgtgca gcgcctggtc gagatcgaga cctaccggat
     1381 gatggcgctg cttggcctgc cgatcgccaa ggaggtcggc gcctggctgt acggcgggga
     1441 aaaacggctg gccgaactga tggaacggat cggccaggcg aagaccccgg aagacgaacg
     1501 cgacgtgctc gcctcgctgt cagcactggc tgcggaagtc gaaaattcgg tggcccgcac
     1561 caccttccgc ttcggtgcgt cgcgcgccta ccacggcctg gtcagccagc gcatcgagga
     1621 actgcgcgaa acgcgcatcc ccggcctgcc gacgctcgac gaattcatgc agcgccgcct
     1681 gctgccggcg atgaagacct gcgaggcgat cagccgccgc caggaggagc tgtccggccg
     1741 ggttgcacgc aacagccagc tcctgcgtac ccgcgtcgac attgaactgg agcggcagaa
     1801 ccaggaactg ctgacccaga tgaaccgccg ggcaaaactg cagttgcgcc tgcaggaaac
     1861 ggtcgagggg ctgtcggtgg tcgtcctcac ctactacggt tcgcaactgg tgcaatacct
     1921 ggccaagggc acgaaggaag tgcaccacct caacaccgac gtcatcaccg ccgtttcgat
     1981 cccgctgatc gccggcctcg tcgcctgggg cacccgccgc atgcggcgca agctggcggc
     2041 cgaggaagcc gccggctgag cccgcttcag tctgccgcca aggccgcgcg cagttgctcg
     2101 aagaccaccg gcttggcgag gaagccggtc atccccgccg cctcgcactg ggcgcgatcc
     2161 tcggcgaaga cgctggcggt cagcgcgatc accggcaccg gccgacgctc gttgtcgcgc
     2221 tcccaggaac ggatctggcg ggtcgcggcg aagccgtcga ggaccggcat ctggcaatcc
     2281 atcagcaccg tctcgtaggc gtgcggcgcc gcctgcaccg cggccaccgc cgccgccccg
     2341 tcgccgacgc tatcgtgggc gacgcccagc ttgtcgagca tggcttcgac gatgcgccga
     2401 ttgaccgcgt tatcctcgac caccagcacc cgcgccggca agcggtcggc ggcgctgatg
     2461 ccacactcgg ccgctatcgg cagcgcctcg gcgctcgcct cgagcacgat ctcgaaccag
     2521 gctcgggtac cgacacccgg cgtgctgtca atgccgacct cgccgtccat caggtgcgcc
     2581 agacggctga cgatcgacag ccccagcccg gagccgccat aggcgcgggt cgatgaggtg
     2641 tcgacctggg tgaagggggt gaacagccgc gcctgcgctt ccgaaggaat gccgatgccg
     2701 gtgtcgcaaa cctcgatgtg caagcggacc tggttgcccg cctggcggaa ggaagcgatc
     2761 acctcgacgc cgccgcgctc ggtgaatttg agggcgttgt ggatcaggtt ggacagcatc
     2821 tgccggaccc gcaccggatc gccgaggcaa gccggcggct cgccttccgg ccattggccg
     2881 cgcagcgtca gaccgcggct ggcggcattt tcggcgtaca gacgcaggct gtccttgacc
     2941 agcatgccgg gcaggaaaga ctggcgctgc agcagcatgc gaccggcctc gaccttcgac
     3001 aggtcgagga tatcgttgag caggacaagc agggtttcgc cagattccat gatgactcgg
     3061 gcgtaatcga gacgttcggc gtcactcatg gtcggcgact gcagcagttg ggccatgccg
     3121 aggatgccgt tgagcggcgt ccggatttcg tgcgacatcg tcgccaggaa acggctcttg
     3181 gcgacgttcg ccgcctctgc cgcctcgcgt gcggcaacca gttggccgac gcggtcggcc
     3241 accgtctgcg acatgacgtt gaaggcggcg ccaagctgac caaggtcgtc gcgcccttcc
     3301 ggcacccgcg ccggcgtcag gttgccctcg gtgacggcac ggctggccgc catcagggcg
     3361 cccaactggc gcgtcatcag gaaaccgatc aacatcagca gcccggccga gagcaggagc
     3421 tcgccaaagg cgatcagcat gccctggccg gccaggctgt tgcgggcctt gatgatcggg
     3481 ccaaggtcga tgccgaaat
//
LOCUS       DAOSDX010000209         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9481_length_3493_cov_3.254799, whole genome shotgun sequence.
ACCESSION   DAOSDX010000209 DAOSDX010000000
VERSION     DAOSDX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9481_length_3493_cov_3.254799"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..270)
                     /gene="infA"
                     /locus_tag="PLN02_05795"
     CDS             complement(4..270)
                     /gene="infA"
                     /locus_tag="PLN02_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOI51871.1"
                     /translation="MAKEELLEMQGVVEDVLPDGRFRVTLENGHQMVAYTAGKMKKHH
                     IRILLGDKVSLELSPYDLSKGRITFRHLETRSSSSAPPPPRRRH"
     gene            complement(284..1180)
                     /locus_tag="PLN02_05800"
     CDS             complement(284..1180)
                     /locus_tag="PLN02_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOI51872.1"
                     /translation="MVRIQFAIDLDYTLNSPGADFVFNIHAAHTASQRIVSESLQLTP
                     QLASAIETEPHTAARFLRLHVASGSLHVGYRATLDIDQHIGDPDHIPETPINQLPLSA
                     LTYIYPSRYCQSDRLAMLAMSEFGHLPKGYRRVEAICHWVNRHVAFRSATSSSTTSAV
                     DTLTERVGVCRDFAHLMIAICRALSIPARFTTGIDYGADPALGPTDFHAYVEVFLDQR
                     WYIFDPSGTAIPMGFVRIGTGRDAADVSYATIFGSVSSPPPLISISALTEPGQTWQIP
                     QRCPQALSTDTPAATQPGCATH"
     gene            complement(1458..3143)
                     /gene="leuA"
                     /locus_tag="PLN02_05805"
     CDS             complement(1458..3143)
                     /gene="leuA"
                     /locus_tag="PLN02_05805"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOI51873.1"
                     /translation="MMAQQKSSKYTAFPPVGLTDRQWPNRVITQAPMWMSTDLRDGNQ
                     ALFEPMNADKKMQMFQTLCAIGFKQIEVAFPSASETEFGFVRRLIEEKHIPADVTIEV
                     LTQAREHLIRRTFESLKGAKQAIVHVYNATCPSFRDFVFGMSKAEVVKMAVDAVKLIK
                     TLAEQMPETKITLEYSPELFTATELDFAKEVCDAVTAAWGATPDNKVILNLPTTVEIA
                     TPNIYADQIEWMHRHLERRDSVIISLHPHNDRGTAVAAAELGLMAGADRVEGCLFGNG
                     ERTGNVDLVTLALNMHTQGVDPQLDFSDINAVARTYEHCTQLPIHPRHPYVGDLVFTA
                     FSGSHQDAIKKGFAAQKADETWSVPYLPIDPADVGRSYDSVIRVNSQSGKGGIAYLME
                     TEHGVVMPRRLQVEFSGVVQKHTDTHGGEVNADDLWRLFAGEYLDAETPVRYREHHLF
                     EHGSQQGIRLTVDIGGTPHILSGEGNGPINAAMHALQGAGIEAQVRSYEERSMVPMGE
                     DGNARAVAFIEMAANGRGERYGVGIDSNIVTASIKALISGINRLGVSALDAAA"
     gene            3216..>3493
                     /locus_tag="PLN02_05810"
     CDS             3216..>3493
                     /locus_tag="PLN02_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOI51874.1"
                     /translation="MILLCFGRKLRDGEFMSNVIQMDRYDKEILAVLQRDGRISNQDL
                     ADRIGLSPSPCLRRVRALEEAGIIAGYRAQLDAKKLGLSLMALIGISM"
BASE COUNT          565 a          941 c         1301 g          686 t
ORIGIN      
        1 ggcttagtgc cggcggcggg gcggcggcgg ggcgctgctg ctgctgcggg tttccaggtg
       61 acggaaggtg atgcggccct tgctcaggtc gtagggcgag agttcgaggc tgaccttgtc
      121 gccgagcagg atgcggatgt ggtgcttctt catcttgccg gcggtgtagg cgaccatctg
      181 gtgcccgttt tccagggtga cgcggaagcg tccatcgggt agaacgtctt cgacgacgcc
      241 ttgcatttca agtagttctt ccttggccaa gcggtattct ccttcaatgg gttgcgcagc
      301 cgggctgcgt tgcggccggg gtatcggtcg acagggcttg tggacagcgc tgcgggattt
      361 gccaggtttg tccgggttcg gtgagggcgc tgatcgagat cagtggcggc ggcgagctga
      421 cgctgccgaa aatcgtcgcg taggaaacgt cggccgcgtc gcggccagtg ccgatacgga
      481 cgaagcccat cggaatcgcg gtccccgagg ggtcgaaaat gtaccagcgc tggtcgagga
      541 aaacctcgac gtaggcgtgg aagtcggttg gcccgagcgc cgggtcggcg ccgtagtcga
      601 tgccggtggt gaagcgcgcc gggatgctca gggcgcggca gatggcgatc atcaggtggg
      661 cgaagtcgcg gcagacgccg acgcgctcgg tcagcgtgtc gaccgccgag gtggtcgagc
      721 tgctggtcgc cgagcggaag gcgacatgac ggttgaccca gtggcagatc gcttcgacgc
      781 ggcgatagcc cttgggcaga tggccgaatt cactcattgc cagcatcgcc agacggtcgg
      841 actggcagta gcggctcggg tagatatagg tcagtgccga caacggcaac tgattgatcg
      901 gggtttccgg gatgtggtcg ggatcgccga tgtgctggtc gatgtcgagc gttgcccggt
      961 aaccgacatg cagcgagccg ctggcaacgt gcaggcgcag gaagcgggcg gcggtgtgcg
     1021 gctcggtttc gatcgccgaa gcgagttgcg gcgtgagctg caggctttcg ctgacgatgc
     1081 gctggctggc ggtatgggcg gcgtggatgt tgaagacgaa atcggcgccg ggagaattca
     1141 gtgtgtagtc gaggtcgatc gcaaactgta tgcgaaccat gcaattccag aatgagcggc
     1201 gcgccgtgct ggccggttca ggctgcgcgg acgggcggtg ggacggcgct atgcaacggg
     1261 aaaatgggcg gttcggcttg ccgctgcctc gcggggcgaa ggcggcaggg gggacggcat
     1321 ttacgcgggc agaacgatga cgatgccgcc aggcggcggc aaggaaaggc acatcttacg
     1381 ccgctgccgg cgaactgaca aggaaagtcc cggaaattca ggcgcttgcc aggttgtcgg
     1441 caagcgcctg gaaggggtca ggcggcggcg tcgagggcgc tgacgccgag gcggttgatg
     1501 ccactgatca aggccttgat cgaggcggtg acgatgttgc tgtcgatgcc gacgccgtag
     1561 cgttcgccgc gaccgttggc cgccatctcg atgaaggcga cggcgcgagc attgccgtcc
     1621 tcgcccatcg gcaccatcga acgctcttcg tagctgcgca cctgggcttc gatgccggcg
     1681 ccctgcaagg cgtgcatggc ggcgttgatc ggaccgttgc cttcgccgct caggatgtgc
     1741 ggcgtgccgc cgatgtccac cgtgaggcgg atgccctgct ggctgccgtg ctcgaacagg
     1801 tggtgttcgc ggtagcggac cggggtttcg gcgtcgaggt attcgccggc gaagaggcgc
     1861 cagaggtcgt cggcgttgac ctcgccgccg tgcgtgtcgg tgtgcttctg gacgacgccg
     1921 gagaactcga cctggaggcg gcgcggcatg accacgccgt gctcggtctc catcaaatag
     1981 gcgatgccgc ccttgcccga ctggctgttg acgcggatca ccgagtcgta ggagcggccg
     2041 acatcggcgg ggtcgatcgg gaggtagggc actgaccaag tctcatccgc cttttgagcg
     2101 gcaaacccct tcttgatggc gtcctggtgg gagccggaga aggccgtgaa aacgagatcc
     2161 ccgacgtatg ggtggcgcgg gtggatcggg agctgggtgc agtgttcgta ggtgcgggcg
     2221 acggcgttga tgtcggagaa gtcgagttgc ggatcgacgc cctgggtgtg catgttgagc
     2281 gcgagggtga cgagatcgac gttgccggtg cgctcgccgt tgccgaagag gcagccctcg
     2341 acgcggtcgg cgccggccat caggccgagt tcggcggcgg cgacggcagt gccgcggtcg
     2401 ttgtgcgggt ggaggctgat gatcacgctg tcgcggcgtt cgaggtggcg gtgcatccac
     2461 tcgatctggt cggcgtagat gttcggcgtg gcaatctcga ccgtggtcgg caagttgagg
     2521 atgaccttgt tgtccggcgt cgcgccccag gcggcggtga ccgcgtcgca gacttccttg
     2581 gcgaagtcga gttcggtggc ggtgaacaat tccgggctgt attcgagggt gatcttcgtt
     2641 tccggcatct gttcggcgag cgttttgatc aacttgaccg cgtcgaccgc catcttcacc
     2701 acctcggcct tgctcatgcc gaagacgaaa tcgcggaagc tcgggcaggt cgcgttgtag
     2761 acatggacga tcgcctgctt ggcgcccttc aaggattcga aggtgcggcg gatgaggtgc
     2821 tcgcgcgcct gggtgaggac ttcgatggtg acgtcggcgg gaatgtgctt ttcctcgatc
     2881 aagcggcgga cgaagccaaa ctcggtttcc gacgctgacg ggaaggcgac ctcgatctgt
     2941 ttgaagccga tggcgcagag tgtctggaac atctgcatct tcttgtcggc gttcatcggc
     3001 tcgaacaagg cctggttgcc gtcgcgcagg tcggtgctca tccacatcgg cgcctgggtg
     3061 atgacgcggt tcggccactg gcggtcggtg aggccgacgg gcgggaaagc ggtgtacttg
     3121 gatgattttt gctgcgccat gatgcggctc cggaaaacgt ttgcgatggc gaaatcttac
     3181 gcaagactgt gcggcaggtg cttgcgtttt gtggcatgat tttgctgtgt tttggcagaa
     3241 aattgcgcga tggtgagttt atgagcaacg ttattcagat ggatcgctac gacaaggaaa
     3301 ttcttgccgt cctgcagcgt gatgggcgga tcagcaacca ggatctcgcc gaccgcatcg
     3361 gcctctcgcc gtcgccttgt ctacgccggg tgcgggcgct cgaagaggcg ggcatcatcg
     3421 ccggctaccg cgcccagctc gatgcgaaaa agctggggct gagcctgatg gcgctgatcg
     3481 gtatctcgat gga
//
LOCUS       DAOSDX010000210         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9546_length_3477_cov_2.750146, whole genome shotgun sequence.
ACCESSION   DAOSDX010000210 DAOSDX010000000
VERSION     DAOSDX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9546_length_3477_cov_2.750146"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLN02_05815"
     CDS             <1..286
                     /locus_tag="PLN02_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOI51875.1"
                     /translation="WKLSPIDMASLDKWDDYTKAKEAMFFHTDTADAPWTVIKSNCKK
                     RARLNAMRYVLHKLPYANKDTRLIGNLDPLIVGRANVVYERGEQSVVPLL"
     gene            complement(302..976)
                     /locus_tag="PLN02_05820"
     CDS             complement(302..976)
                     /locus_tag="PLN02_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI51876.1"
                     /translation="MDDMTPITEIAPFRNLSDAGRALLAQVQVSVHMRSPQALIHKGQ
                     AASGAYFVLAGRLRVYALAPNGNEATLYFIGPGETCVFALNSLFNDLRYPAWVQAVED
                     TRLATIPGPVYRRLFAVEPEVRDLTVRTLSTLVFRLMAELEEVHAYRLNQRLANAILV
                     HGSGEGLLRMTQQQLADHLGTTREVIARLIGELVDAGLIESRRACIRIADAAGLARLI
                     AGDAAV"
     gene            complement(987..2300)
                     /locus_tag="PLN02_05825"
     CDS             complement(987..2300)
                     /locus_tag="PLN02_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOI51877.1"
                     /translation="MNSGLSDFLMIAIPAILTGGIVLALMVRRELAPAVGLRGEQRWL
                     LGLALGMGLVAFSIKLGVALILSNAPGATVEPLAARDFGSAVMPDDDSVALIKAFINP
                     ASGNGQRYVWERLPQEAPAPAGLPTTATRVALGERLFNERRLSGDGTLNCASCHDLTG
                     HAGADGRPLARGIADQVGPRNTPTVWNAAFQSVLFWDGRAPSLEAQAGGPILNPIEMG
                     LASAEEAERRLEGDTDYRRLFTAAFGDAAVTFPRIVQALAAYERTLITPDSPYDRFVD
                     GDAGALSPAQLRGMALFEKLGCILCHRGPNFSDASLLGGQSALRYFPANPTPYEKRYP
                     LLGADGRPGVWRVPSLRNVALTGPWLHNGSVDQLDEVVRIMASAQLGRSRQLQAWIDG
                     ESALHLADQTPLPDAQVADLVAFLEALSSDRLRRQAGLPEDTRAE"
     gene            2546..3244
                     /locus_tag="PLN02_05830"
     CDS             2546..3244
                     /locus_tag="PLN02_05830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOI51878.1"
                     /translation="MKKPFVSLFLAVPLLFSAVPASAGGGAALHFEPGRSALVVTDPQ
                     NDFLREDGKLYGLLKDNLARLDTVANLERLMQAAKAGGYLFAVDPLVYGALDGQWTQA
                     GALQRQLLDMGALRRDAAGGGDFYARFKPYIDDPATVVVAPHKLYGPESNDLIYQLRS
                     RGIDTVVLAGLVANLCVDSHLRALMENGFRVYVVKDAVGAPGEAAYQAALVNFGMIAN
                     GVLSTAEAVAALRR"
     gene            complement(3251..>3477)
                     /locus_tag="PLN02_05835"
     CDS             complement(3251..>3477)
                     /locus_tag="PLN02_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOI51879.1"
                     /translation="EKFCGETAEQVAHRFGRIGTIDLRTDFLHQGIGHEFTATGRIVR
                     LGGRIASIQMTLENESGLLIATGSAAYVVS"
BASE COUNT          531 a         1153 c         1256 g          537 t
ORIGIN      
        1 gtggaagctg tcgcccatcg acatggcctc gctcgacaag tgggacgact acaccaaggc
       61 caaggaggcg atgttcttcc acaccgacac cgccgatgcg ccatggacgg tgatcaagtc
      121 gaactgcaag aagcgggcgc ggctcaacgc catgcgctac gtcctgcaca agctgccgta
      181 tgcgaacaag gacacccggc tgatcggcaa cctcgatccg ctgatcgtcg gccgcgccaa
      241 cgtcgtctat gaacgcggcg aacagtcggt ggtgcccctc ctctaagccg agcggcggcg
      301 atcagaccgc cgcgtcgccg gcgatcaggc gggcgaggcc ggcagcgtcg gcgatgcgga
      361 tgcaggcgcg gcggctctcg atcaggccgg cgtcgaccag ttcgccgatc aggcgggcga
      421 tcacctcgcg cgtcgtgccg aggtgatcgg ccagttgctg ctgcgtcatg cgcagcagcc
      481 cctcgccgct gccgtgcacc aggatggcgt tggccagccg ctggttcagc cggtaggcgt
      541 gcacttcctc gagttcggcc atcagccgga agaccagcgt ggacagggtg cgcaccgtca
      601 gatcgcggac ctcaggctcg accgcgaaca ggcgccggta gaccggtccc gggatggtcg
      661 ccagccgggt gtcctcgacc gcctgcaccc aggccgggta gcgcaggtcg ttgaacaggc
      721 tgttcagcgc gaagacgcag gtttcgccgg ggccgatgaa atacagcgtg gcctcgttgc
      781 cgttcggcgc cagcgcatag acgcgtagcc ggccggcgag cacgaagtag gcgccggacg
      841 ccgcctgccc cttgtggatc agcgcctgcg gcgagcgcat atggacgctg acctggacct
      901 gcgccagcaa ggcccggccg gcgtcggaca ggttgcggaa gggggcgatt tcggtgatcg
      961 gggtcatgtc gtccatgccc gccagcttat tccgcgcggg tatcctccgg caagcccgcc
     1021 tggcggcgca ggcggtcgct gctcagcgct tcgaggaagg cgaccaggtc ggcgacctgg
     1081 gcgtcgggca gcggcgtctg gtcggcgagg tgcagcgccg actcgccgtc gatccacgcc
     1141 tgtagctggc gcgagcggcc gagctgggcg ctggccatga tgcggacgac ttcgtcgagt
     1201 tggtccaccg agccgttgtg caaccagggg ccggtcagcg cgacgttgcg tagcgacggc
     1261 acgcgccaga cgccggggcg gccgtcggcg ccaagcagcg ggtagcgctt ctcgtaaggc
     1321 gtcggattgg ccgggaagta gcgcagcgcg ctctggccgc cgagcaggct ggcgtcgctg
     1381 aagttcggcc cgcggtgaca gaggatgcag cccagcttct cgaacagcgc catgccgcgt
     1441 agctgcgccg ggctgagggc gccggcgtcg ccatcgacga agcggtcgta ggggctgtcc
     1501 ggcgtgatca gcgtgcgctc gtaggcagcc agcgcctgga cgatgcgcgg gaaggtgacg
     1561 gctgcgtcgc cgaaggcggc ggtgaacaag cggcgatagt cggtgtcgcc ctccaggcgg
     1621 cgctcggctt cttccgccga ggccaggccc atctcgatcg ggttgaggat gggaccgccc
     1681 gcctgtgctt ccagcgaggg cgcccggcca tcccagaaca gtaccgactg gaaagcggcg
     1741 ttccatactg tcggcgtgtt gcgcggtccg acctggtcgg cgatgccgcg cgccagcggc
     1801 cgcccgtcgg cgccggcatg gccggtcaag tcgtggcagg aagcgcagtt cagcgtgccg
     1861 tcgccggaca gccggcgttc gttgaacagg cgttcgccga gcgccacccg ggtcgccgtc
     1921 gtcggcaggc cggccggcgc cggggcctct tgtggcaggc gctcccagac gtagcgctgg
     1981 ccgttgcccg aggccgggtt gatgaaggct ttgatcagcg cgacgctgtc gtcgtcgggc
     2041 atgacggcgc tgccgaaatc gcgcgcggcg agtggttcga cggtggcgcc gggggcgttg
     2101 ctgaggatga gggcgacgcc cagcttgatg ctgaaggcga ccagccccat gcccagcgcc
     2161 agccccagca gccagcgttg ttcgccgcgc aggccgacgg ctggtgccag ttcgcggcgg
     2221 accatcaggg caaggacgat gccgccggtc aggatggccg ggatggcgat catcaggaaa
     2281 tcggaaaggc cgctgttcat ggccggctcc cggtgaagct ggcggcattc ggggcggatg
     2341 gggcgggcat gaggcactcc ttggtcgggg atctgaacgc aggctagccc gcgccggccc
     2401 gccgttctgt agcaaaagct acgttggcgc gggatggcgg cttgtgtagc caaagctacg
     2461 gaatgcgctg gccgggctgc cgagaatgcc gccaggccgt ccatgccggg cggccgcaat
     2521 ccctgtccga tggaaaggat gcatcatgaa aaagccattc gtctcgctct tccttgccgt
     2581 gccgctgctc ttctccgccg ttcccgccag tgccgggggc ggtgccgcgc tgcacttcga
     2641 acccgggcgc agcgccctcg tcgtcaccga tccgcagaac gatttcctgc gcgaggacgg
     2701 caagctctac gggctgctca aggacaatct cgcccgcctc gacaccgtcg ccaacctgga
     2761 gcgcctgatg caggcggcga aggccggcgg ctacctgttc gccgtcgatc cgctggtcta
     2821 tggcgcgctc gacggccagt ggacgcaagc cggcgcgttg cagcgccagt tgctcgacat
     2881 gggggcgcta cgccgcgacg ccgccggcgg tggcgacttc tacgcccgct tcaagcccta
     2941 catcgacgat ccggcgaccg tcgtcgtcgc cccgcacaag ctctacggcc cggagagcaa
     3001 cgatctgatc taccagttgc gcagccgcgg catcgacacc gtggtgctgg ccgggctggt
     3061 cgccaacctg tgcgtcgact cgcacctgcg ggcgctgatg gagaacggct ttcgcgtcta
     3121 tgtcgtcaag gatgccgtcg gcgcgcccgg cgaagcagcc taccaggcgg cgctggtcaa
     3181 cttcgggatg atcgccaacg gcgtgctcag caccgccgag gcggtcgccg cgctgcgccg
     3241 ctgaacgcgc tcagctgacc acgtaagcgg cgctgccggt ggcgatgagc agcccgcttt
     3301 cgttttccag cgtcatctgg atcgaagcga tgcggccgcc gagccgaacg atgcggccgg
     3361 tggccgtgaa ctcgtggccg atgccctggt gcaggaaatc ggtgcgcagg tcgatggtgc
     3421 cgatgcggcc gaagcggtgc gccacctgtt cggcagtctc gccgcagaac ttctcgg
//
LOCUS       DAOSDX010000211         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9571_length_3470_cov_4.104246, whole genome shotgun sequence.
ACCESSION   DAOSDX010000211 DAOSDX010000000
VERSION     DAOSDX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9571_length_3470_cov_4.104246"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLN02_05840"
     CDS             complement(<1..343)
                     /locus_tag="PLN02_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HOI51880.1"
                     /translation="MSYYDSLETRDPAVREADLMDKLRSQLAHAKQASTYYAHVLAGI
                     DPVACDSRQALAALPLTRKRELIELQKATPPFGGLNALAKGEVRRVFASPGPIFELQG
                     SEVDHWRMVRAL"
     gene            complement(505..1329)
                     /locus_tag="PLN02_05845"
     CDS             complement(505..1329)
                     /locus_tag="PLN02_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI51881.1"
                     /translation="MNTQAATADNYLSINNIEVIYDHVILVLKGVSLAVPKGKIVALL
                     GANGAGKSTTLKAISNLLRAERGDVTKGSVEYKGQRIDQLSPNELVKRGVIQVMEGRH
                     CFGHLTIEENLLTGAYTRSISRGELKDSLDKVYHYFPRLKTRRTSQAGYTSGGEQQMC
                     AIGRALMAKPEMILLDEPSMGLAPQIVEEIFEIVKDLNAREQVSFLLAEQNTMVALRY
                     ADFGYILENGRVVMEGDAEELRTNEDVKEFYLGLSSAGRKSFKDVKHYRRRKRWLS"
     gene            complement(1387..2706)
                     /locus_tag="PLN02_05850"
     CDS             complement(1387..2706)
                     /locus_tag="PLN02_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI51882.1"
                     /translation="MKKVFKPAVAAAAVSLAMLSQAAYSAEQFFPIPSYRVGAYGANG
                     QAFYGGFIDYLNYVNMKDKGVNGVTLTWEECETEYNNAKGVECYERMKGKAAQSAGPI
                     HTMSTGISYALIDKSAQDKLPLAMMGYGRTDAVDGSVFPYAFPLVTTYQMQASAIVKF
                     VKDKLGGSLAGKKIVYLYHDSAYGKEAIVALNAEAGLNKFELVQIPVAHPGNEQGAQW
                     LKIRQEKPDFVIFWGWGVMNQTALKAAQKVGFPRDKMIGSWWTGSEEDVIPAGEAAKG
                     YMAATWNVAGKQVPVIADIEKVVYGAGKGNLQDKGKIGTILYNRGVSAAVLSVEAIRK
                     AQEKYGKGKAMNGTQVAWGLENLDITDARLKALGATNLLPTIKTSCANHEGSGKVKIQ
                     QWDGAKWVPVSDWIEGNKGLIHPLFQASAKQYAKEKGITPRDCSKEK"
     gene            complement(2746..>3470)
                     /locus_tag="PLN02_05855"
     CDS             complement(2746..>3470)
                     /locus_tag="PLN02_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI51883.1"
                     /translation="ILFGLPSLRIKGFYLAVATLAAQFFIVWCLTKFPWLSNNSSSGV
                     ISAQNIEILGYAFDTPRDKYLLVLAIVVLLALAAKNMVRSTTGRAWMAVRDMDVAASV
                     IGIRLMRTKLLAFAVSSFYCGVAGALYAYCYLGSVEPDGFSLDMSFRILFMVIIGGVG
                     SILGSFLGAAFILLLPIFLDIALPFFAGLFGLPFSSATVSHIQILVFGALIMFFLIVE
                     PHGLARLWQIAKEKLRLWPFPH"
BASE COUNT          608 a         1089 c         1108 g          665 t
ORIGIN      
        1 aaagcgcccg caccatgcgc cagtgatcga cctcgctccc ctgcaattcg aagatcggcc
       61 cgggcgacgc gaagacccgg cgcacctctc ccttggccag cgcgttaagg ccgccaaacg
      121 gcggggtcgc cttctgcaat tcgatcaact cgcgcttgcg cgtcagcggc agtgctgcca
      181 gcgcctggcg tgagtcgcag gccaccggat caatcccggc cagcacgtgt gcgtagtaag
      241 tgctcgcctg cttggcatgc gccaactggc tgcgcaactt gtccatcaga tcggcctcgc
      301 gcaccgccgg atcacgggtt tcgagcgaat cgtagtagct catttcattt ttctccgttg
      361 ttcacggtcg gccatcggaa agatgttggt ggggcgagca gcgggttgcc aagtagtcgc
      421 tcagggcaag gcggagcggg gcgccgtttg ctgctgcaaa caagtctcgt gacaacgcag
      481 ccatgagcgg cgcacggcaa cccgttacga cagccagcgc ttgcggcggc ggtagtgctt
      541 gacgtccttg aacgacttcc gccccgccga cgagaggcca aggtagaact ccttcacatc
      601 ctcattcgtc cgcagctcct ccgcgtcgcc ttccatcacc acccgcccgt tttccaggat
      661 gtagccgaaa tcggcgtagc gcagggcgac catggtgttc tgttcggcaa gcaggaagct
      721 gacctgctcg cgggcgttga ggtccttcac gatctcgaag atttcctcga cgatctgcgg
      781 cgccaggccc atcgacggct cgtcgagcag gatcatttcc ggcttggcca tcagggcccg
      841 gccgatggcg cacatctgct gctcgccgcc cgaggtgtag cccgcttgcg aggtgcgccg
      901 cgtcttcagg cgcgggaagt agtggtagac cttgtccagg ctgtccttca gctcgccgcg
      961 cgagatgctg cgcgtgtagg caccggtcag caggttttcc tcgatggtca ggtggccgaa
     1021 gcaatgccgg ccttccatga cctggatgac gccgcgcttg accagttcgt tgggcgagag
     1081 ctggtcgatg cgctgcccct tgtattcgac cgagcccttg gtcacgtcgc cgcgctcggc
     1141 gcgcagcagg ttggaaatcg ctttcagcgt cgtcgatttg ccggcgccgt tggcgcccag
     1201 cagggcgacg atcttgcctt tcggcacggc cagcgacacc cccttgagga ccaggatcac
     1261 gtggtcgtag atgacctcga tgttgttgat gctcaggtag ttgtcggccg ttgcagcttg
     1321 tgtgttcatc tcttctcccg tcacggaagg aagcgccggg cgcggcagcg cgcccggcgg
     1381 cccggcttat ttttccttgc tgcagtcacg cggcgtgatg cccttttcct tggcgtactg
     1441 cttggcggac gcctggaaca acgggtggat cagccccttg ttgccctcga tccagtcgga
     1501 caccggcacc cacttggcgc cgtcccactg ctgaatcttc accttgccgg aaccttcgtg
     1561 gttggcgcac gaggtcttga tggtcggcag caggttggtg gcgcccagtg ctttcaggcg
     1621 ggcgtcggtg atgtcgaggt tttccagacc ccaggcgacc tgggtaccgt tcatcgcctt
     1681 gcccttgccg tacttctcct gcgccttgcg gatcgcctcg accgacagca cggcggcgga
     1741 aacgccgcgg ttgtaaagga tggtgccgat cttgcccttg tcctgcaggt tgcccttgcc
     1801 ggcgccgtag accaccttct cgatgtcggc gatcaccggc acctgcttgc cggcgacgtt
     1861 ccaggtcgcc gccatgtagc ccttggccgc ttcgccggcc gggatgacgt cttcctcgga
     1921 accggtccac cacgagccga tcatcttgtc gcgcgggaag ccgaccttct gcgcggcctt
     1981 cagtgccgtc tggttcatca cgccccagcc ccagaaaatc acgaaatcgg gcttttcctg
     2041 gcgaatcttc agccactgcg cgccctgctc gttgcccgga tgggcgaccg ggatctgcac
     2101 cagttcgaac ttgttcaggc cggcctcggc attgagcgcg acgatcgctt ccttgccgta
     2161 ggcggagtca tggtagagat agacgatctt cttgccggcc aggctgccgc ccagcttgtc
     2221 cttgacgaac ttgacgatgg ccgaggcctg catctggtag gtggtgacca gcgggaaggc
     2281 gtaggggaag accgagccgt cgaccgcgtc ggtgcggccg tagcccatca tcgccagcgg
     2341 cagcttgtcc tgcgccgact tgtcgatcag cgcgtaggaa atgccggtcg acatggtatg
     2401 gatcggcccg gccgactgcg ccgccttgcc cttcatccgc tcgtagcact cgacgccctt
     2461 cgcgttgttg tactcggttt cgcattcttc ccaggtcagg gtgacgccat tgacgccctt
     2521 gtccttcatg ttgacgtagt tcaggtagtc gatgaagccg ccgtagaagg cctggccgtt
     2581 ggcgccgtag gcaccgacgc ggtagctcgg gatggggaag aattgttcgg ccgagtaggc
     2641 agcctgcgac agcatggcca gggaaaccgc ggcagcggca acagcgggtt tgaaaacttt
     2701 tttcatgtgt atgtctcctc cagtgttacg aaaaacggcc gtttctcagt gcgggaacgg
     2761 ccacaggcgc agcttctcct tggcgatctg ccacagacgg gccaacccgt gcggctcgac
     2821 gatcaggaag aacatgatca gcgcaccgaa caccaggatc tggatgtgcg acacggtggc
     2881 gctggaaaac ggcaggccga acaggccggc aaagaacggc agggcaatgt cgaggaagat
     2941 cggcagcagc aggatgaagg cggcgccaag gaaggaaccg aggatggagc cgacgccacc
     3001 gatgatgacc atgaacagga tgcggaagga catgtcgagc gagaagccgt ccggttcgac
     3061 cgaacccagg tagcaatacg catagagcgc gccggcgacg ccgcagtaga aggaactgac
     3121 ggcgaaggcg agcagcttgg tgcgcatcag gcggatgccg atcaccgagg cggcgacgtc
     3181 catgtcgcgc accgccatcc aggcccggcc agtggtcgag cggaccatgt tcttggccgc
     3241 cagggcgagc agaacgacga tcgccaggac cagcaggtac ttgtcgcgcg gcgtgtcgaa
     3301 cgcgtagccg aggatttcga tgttctgcgc cgagatcacg ccggacgacg agttgttcga
     3361 cagccagggg aacttggtca ggcaccagac gatgaagaac tgcgccgcca gcgtcgccac
     3421 cgcgagataa aaacccttga tgcgcaagct gggcaggccg aacaggatgc
//
LOCUS       DAOSDX010000212         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9582_length_3468_cov_3.071491, whole genome shotgun sequence.
ACCESSION   DAOSDX010000212 DAOSDX010000000
VERSION     DAOSDX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_3468_cov_3.071491"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLN02_05860"
     CDS             <1..635
                     /locus_tag="PLN02_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOI51884.1"
                     /translation="AGTFNMLRANDLIWSFVVNNYLMGKDPFPFDLLYWNSDSTRMPA
                     AMHSFYLRNMYLENKLKDPGGIELGGVKIDIRKVKTPCYFISTIEDHIAPWKSTYMGA
                     RLPTGKTKFVLGGSGHIAGIVNPPAANKYGYWTNDATDGNLPESPEDFLAGATQHAGS
                     WWTHWQEWVTALPGGSAKVPARDPAAGALKALEEAPGSYVKFRLDAQKQD"
     gene            773..1438
                     /locus_tag="PLN02_05865"
     CDS             773..1438
                     /locus_tag="PLN02_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI51885.1"
                     /translation="MKRTMIALCAVSLAFTGCANMDETQRHTGTGAAIGAVAGAVLGA
                     ATSSRSDRGKGAVIGAAAGAAIGAGGGYLWSKRMQEQKAAMEQATQGTGVEVTQTADN
                     RLKLEIPSDVSFDSGSYAIRPSFRPVLDKLAAGLAEHAVTRVTIVGHTDSSGSDKVNN
                     PLSVKRAEAVRDYLVPRGVAASRIAVDGRGAREPLADNATAEGRAKNRRVEIFIAEGQ
                     AAN"
     gene            complement(1481..1933)
                     /locus_tag="PLN02_05870"
     CDS             complement(1481..1933)
                     /locus_tag="PLN02_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOI51886.1"
                     /translation="MSPHFVIPRFRLPALIAEIGHKLPQWPHSLALVTALNAALKLKL
                     LPESELGALEDKLFRVSVLDTGGVAHYTFRNGLFRPVFSVQREPDLAFAANLSAYLQL
                     LARQEDPDTLFFNRELEITGDTELGLIVKNMLDAIEWPTLPRLPLPGN"
     gene            complement(1930..2838)
                     /locus_tag="PLN02_05875"
     CDS             complement(1930..2838)
                     /locus_tag="PLN02_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOI51887.1"
                     /translation="MKLSLGPLLWFWPKAEVMEFYAAMAENPALDIIHVGEVVCSRRQ
                     QLRTADWTGLARDLTDAGKEVVISAQALLESESDLKALRRIVDEAGCKVEANDLGAIN
                     LVSGQDFVAGPHLNIYNEATLATFARYGMKRWVPPLEASRPLIETLHASRPAGVETEV
                     FAFGKIPLAFSARCFTARHYELNKDDCQFKCLDHAEGLTLKTREGQAFLTINGIQTMS
                     AQTYCLLGEMPTLRELGIDIVRLSPQKNRIDEIIAAFAAARNGQTPTVDTAAWSECGL
                     VDGYWFGDAGIATHHIPQPARTGAPA"
     gene            complement(2841..>3468)
                     /locus_tag="PLN02_05880"
     CDS             complement(2841..>3468)
                     /locus_tag="PLN02_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOI51888.1"
                     /translation="EAINFCRREFGIRRAVLPRVLTLAQVENVIRNTEVEIEVFGFGS
                     LCVMNEGRCWLSSYACGESPNTVGACSPAKYVKWDKQPGAMETRLNGILIDRFGDSEP
                     AGYPTLCKGRFDVRGETYYALEEPTSLNVLEILPEIVKIGVRAIKVEGRQRSPTYVTQ
                     VTRTLRAALDALRDGAERYHVKPAWQAELAKVSEGNQATLGAYNRPWR"
BASE COUNT          596 a         1070 c         1198 g          604 t
ORIGIN      
        1 tggccggcac cttcaacatg ctgcgcgcca acgacctgat ctggtccttc gtggtcaaca
       61 actacctgat gggcaaggac cccttcccct tcgacctgct ctactggaac tcggactcga
      121 cgcgcatgcc ggcggcgatg cacagcttct acctgcgcaa catgtacctc gagaacaagc
      181 tcaaggaccc cggcggcatc gaactgggcg gcgtcaagat cgacatccgc aaggtcaaga
      241 ccccgtgcta cttcatctcg accatcgaag accacatcgc gccgtggaag agcacctaca
      301 tgggcgcccg tctgccgacc ggcaagacca agttcgtcct cggcggctcc ggccacattg
      361 ccggcatcgt caatccgccg gcagccaaca agtacggcta ctggaccaac gacgcgaccg
      421 acggcaacct gccggaaagc ccggaggatt tcctggccgg cgcgacgcag cacgccggtt
      481 cgtggtggac ccactggcag gaatgggtga ccgcactgcc cggcggttcg gccaaggtgc
      541 cggcccgcga tccggcggcc ggagcgctca aggcgctgga agaggctccg ggcagctacg
      601 tcaagttccg tctcgacgcg cagaagcagg actgaacctt tccgtcctgc ggcaatcaac
      661 gagggaggcg caagcctccc tttttgctgg ctgttacaac tgctaacagc ccggatcggt
      721 ggccgtgcta gtctgcaaaa gccggacagg accggcgaca ggaggtaaaa cgatgaaacg
      781 aacgatgatt gccctgtgtg ccgtcagtct ggcattcaca ggttgtgcca acatggatga
      841 aacgcaacgc cataccggaa ccggtgcggc gattggcgcc gttgccggtg ccgtactcgg
      901 cgccgccacg tccagcaggt ctgaccgcgg caagggcgcg gtgatcggcg ccgccgccgg
      961 cgcggcgatc ggtgccgggg gtggttacct gtggtcgaag cgcatgcagg agcagaaggc
     1021 ggcgatggag caggcgacac agggcaccgg cgtcgaagtg acgcagacgg ccgataatcg
     1081 cctcaagctc gagattccca gcgatgtttc cttcgattcc ggcagctacg cgatccggcc
     1141 atcgtttcgg ccggtgctcg acaagctggc tgccggcctt gccgaacacg cggtgacgcg
     1201 ggtgacgatc gtcggccata ccgacagttc cggctcggac aaggtcaaca atcccttgtc
     1261 ggtgaagcgg gcggaggcgg tgcgcgacta tctggtaccg cgcggtgtgg cggcgtcgcg
     1321 gatcgcagtc gatgggcgcg gagcgcgtga gccgctggcc gacaacgcga cggcggaagg
     1381 ccgggcgaag aaccggcggg ttgaaatctt cattgccgag ggccaggcag ccaactgagc
     1441 cgagcttccc cggtcacccg gcccgccgcg cgcgggccgc tcagtttccg ggtagcggca
     1501 ggcgtggcag ggttggccac tcgatcgcgt cgagcatgtt cttgacgatc aggccaagct
     1561 cggtgtcacc ggtgatttcg agttcgcggt tgaagaacag ggtgtcgggg tcttcctggc
     1621 gtgccagcag ttgcaggtag gccgacaggt tggcggcgaa ggccaagtcc ggttcgcgtt
     1681 gcacgctgaa gaccggccgg aacaggccgt tgcggaaggt gtagtgggcc acgccaccgg
     1741 tatcgagaac gctgacccgg aacagcttgt cctccagcgc gccgagttcg ctttccggca
     1801 gcagcttgag cttgagggcg gcgttgagtg ccgttaccag agccagcgaa tgcggccatt
     1861 gcggcagctt gtggccgatc tcggcgatca gggccggcag ccggaaacgg ggaatgacga
     1921 aatgcgggct catgcggggg ctccggtgcg ggcgggttgc gggatgtgat gcgtggcgat
     1981 cccggcgtcg ccgaaccagt aaccgtcgac cagaccgcat tcgctccacg ccgccgtatc
     2041 gacggtgggc gtctggccgt tccgggcggc ggcgaaggcg gcgatgattt cgtcgatccg
     2101 gttcttctgc gggctgagac ggacgatgtc gatgccaagt tcgcgcagcg tcggcatctc
     2161 gccgagcagg cagtaggtct gcgccgacat cgtctggatc ccgttgatgg tcaggaaggc
     2221 ctggccttcg cgggtcttca gcgtcagtcc ttcggcatgg tcgagacact tgaactggca
     2281 gtcgtccttg ttcagctcgt aatggcgggc ggtgaagcag cgtgcggaga aggcgagcgg
     2341 aatcttgccg aaggcgaaca cttccgtttc gacaccggcc gggcggctgg cgtgcagcgt
     2401 ttcaatcagc gggcgcgagg cttccagcgg cggcacccag cgcttcatgc cgtagcgggc
     2461 gaaggtggcg agggtggctt cgttgtagat gttgaggtgc gggccggcga cgaaatcctg
     2521 accggagacc agattgatcg ccccgagatc gttggcttcg accttgcagc cggcttcatc
     2581 gacgatacgg cgcagcgcct tgaggtcgct ttccgattcg agcagcgcct gcgccgaaat
     2641 gacgacttcc ttgccggcat cggtcaggtc gcgagccagg ccagtccagt cggcggtgcg
     2701 caactgctgg cggcgcgagc agacgacctc gccgacgtgg atgatgtcga gcgccgggtt
     2761 ctccgccatc gccgcgtaga actccatgac ttctgccttg ggccagaacc agagcagggg
     2821 gccgagcgac agtttcatgc ttatctccag ggccggttgt aggcgcccag cgttgcctgg
     2881 ttgccttcgg aaaccttggc cagttcggcc tgccaggccg gcttgacgtg gtagcgttcg
     2941 gcgccgtcgc gcagcgcgtc gagcgcggcg cgcagggtgc gggtgacctg ggtgacgtag
     3001 gtcgggctgc gctggcggcc ttcgaccttg atcgcgcgca cgccgatctt gacgatctcc
     3061 ggcaggattt ccaggacgtt gaggcttgtc ggttcttcca gcgcgtagta ggtttcgccg
     3121 cgcacatcga agcggccctt gcacagcgtc gggtagccgg ccggttcgct gtcgccgaaa
     3181 cggtcgatca gaatgccgtt gaggcgggtt tccatggcgc ccggttgctt gtcccacttg
     3241 acgtacttgg ccggcgagca ggcgccgacg gtattgggcg attcgccgca ggcgtaggac
     3301 gacagccagc aacgcccctc gttcatcacg cacaggctgc cgaagccgaa gacttcgatt
     3361 tcgacctcgg tgttgcggat cacgttctcg acctgcgcca gcgtcagcac gcgcggcagt
     3421 acggcgcggc ggatgccgaa ctcgcggcgg cagaagttga tcgcctcg
//
LOCUS       DAOSDX010000213         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9596_length_3464_cov_3.385157, whole genome shotgun sequence.
ACCESSION   DAOSDX010000213 DAOSDX010000000
VERSION     DAOSDX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9596_length_3464_cov_3.385157"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..66)
                     /locus_tag="PLN02_05885"
     CDS             complement(<1..66)
                     /locus_tag="PLN02_05885"
                     /inference="COORDINATES: protein motif:HMM:NF022058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51889.1"
                     /translation="MVVELLLSEHNGECQTCDRRED"
     gene            complement(112..825)
                     /locus_tag="PLN02_05890"
                     /pseudo
     CDS             complement(112..825)
                     /locus_tag="PLN02_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
     gene            842..1993
                     /locus_tag="PLN02_05895"
     CDS             842..1993
                     /locus_tag="PLN02_05895"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOI51890.1"
                     /translation="MHRLWLIFAQTVTVAVAVLFTLSTLKPEWLPSRGQRVVALQTAQ
                     PSEDGQPAPAGSYRDAARAALPSVVHIYTTQQVRQQRHPLFDDPIFRHFFGDRQDGET
                     QRNSGLGSGVIVSQNGYILTNYHVIEAADDIQVSLNDGKTYRARTVGSDPESDLAVLR
                     IDAKELPAITFGQVDNLRVGDVVLAIGNPFGVGQTVTMGIVSALGRSHLGINTFENFI
                     QTDAAINPGNSGGALIDSVGNLVGINTAIYSRTGGNHGIGFAIPASSARSIMEQIIEN
                     GSVTRGWIGVEAQEITAELAESFGLPGTEGSLIAGVVRGSPADKAGIRPGDVLLSIDG
                     KPVGDPQVMLDLIAELKPESSAAFRLRRDKSILELQVGIGKRPPLRNAE"
     gene            1999..2799
                     /locus_tag="PLN02_05900"
     CDS             1999..2799
                     /locus_tag="PLN02_05900"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOI51891.1"
                     /translation="MCQLLGMNCNVPTDICFSFSGFQARGGATDVHADGWGIAFFEQR
                     GARLFLDPQPSARSPIAELVARYPIRSQNVIAHIRKATQGSVRLENTHPFMRELWGRY
                     WIFAHNGNLLDFSPKLDGSFLPVGDTDSERAFCWLLQEFRRSFGEAPPAPAALFDRLR
                     ELTLLLAAHGECNFLLSNGDCLFAHASTRLSYIVRQAPFAEAHLKDQDLTVDFREVTT
                     EKDRVAVIATLPLTDNEAWTEMPPGSLWRFHDGAVVGQCPTRPGPPRR"
     gene            complement(2803..>3464)
                     /gene="tatC"
                     /locus_tag="PLN02_05905"
     CDS             complement(2803..>3464)
                     /gene="tatC"
                     /locus_tag="PLN02_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOI51892.1"
                     /translation="EGTKMVAIGVVTPFMVPLKVTAMVAFVVALPFILFQAWGFVSPG
                     LYEHERRLGIPLIVSSTLLFLLGMAFCYYFVFGQVFHFIAGFAPKSITPAPDIEAYLS
                     FVMTMFLAFGLAFEVPVALVVLVKLRVVSVDKLKEWRSYFIVGAFVVAAVVTPPDVVS
                     QLALAVPMCLLYELGLLAARLVSKPAPEAAEDDGAAKVEAELDAEMAQAEAEFRKLTE
                     K"
BASE COUNT          652 a         1202 c         1031 g          579 t
ORIGIN      
        1 gtcttcccgc cggtcgcagg tctggcactc gccgttgtgc tccgacagaa gcagctcgac
       61 caccattcgc cgcgcctcgc ggacgcgccg cgtgttcgtg tgcaccttca tgccctcggc
      121 cgcgggcatc gaacacgacg cggccagcgc ctgcacgccg tagcgctccg tggcgtggtg
      181 cccggcgccc agataggcga cgccgctttc ctcggccaga tgcagggtcg gctccgatac
      241 ctcaccggag agatagagat cgaccccaag ctcaatcgcc tgttcgaaat acccctgagc
      301 ccccccggag caccaggcca cccgcctgat gctgcgagcg ggatcgccca gcaacaaggg
      361 ctgacggcca agtaccgtcg ccaccccggc cgccacctcg gccaacgacg ccggcgcagg
      421 caggctgccg taccagccga tctcctgttc gccgaaacgc ccctccggct gccagccgaa
      481 gcgggcggcc agttgcgcgt tgttgcccag ttcgggatgc gcgtcgagcg gcaggtggta
      541 ggcaaacagg ctgagatcgc tcttgagcag ggtcgccagt cggcgccgcc gcaggccggt
      601 gacgcggggg tcctcacctt tccagaaata accatggtgg acgagcaccg catcggcgtt
      661 gcgggcgacc gcaaggtcga gcagcttctg actggcggta accccggcga cgatgcgccg
      721 cacttcgtcc cgaccttcca cttgcaggcc gtttgggcaa taatcccgga acttcgcggc
      781 ttgcagcaac tcatccagat accgcgccaa ctcttcgcgt ctcatcgtct cgcactttct
      841 catgcatagg ttgtggctga tttttgcaca aacggtcacc gttgcggtgg ccgtcctctt
      901 cacactgtcg acgctgaagc ctgagtggtt acccagccgt ggacaacggg tggtcgctct
      961 gcagacggcg caacccagcg aagacggaca gccggctccg gccggctcct atcgtgatgc
     1021 ggcgcgcgcc gcgctgccgt cagtggttca tatctacacg acacagcagg tccgccagca
     1081 acgccacccc ttgttcgacg acccgatctt ccgccacttc ttcggcgacc gtcaggacgg
     1141 cgaaacccaa cgcaattccg gtctcgggtc cggcgtcatc gtcagccaga acggctatat
     1201 cctgaccaat taccacgtca tcgaagccgc cgacgacatc caggtttcgc tcaacgacgg
     1261 caagacctac cgcgcccgca ctgtcggcag cgacccggaa tccgatctcg ccgtcctgcg
     1321 tatcgatgcc aaggagctgc cggcgatcac cttcggccag gtcgacaacc tgcgtgtcgg
     1381 cgatgtcgtc ctcgccatcg gcaacccctt cggcgttggc cagaccgtga cgatgggcat
     1441 cgtttccgcg ctcggccgct cgcacctggg catcaacacc ttcgagaact tcatccagac
     1501 cgatgctgcg atcaaccccg gcaattcggg aggcgccctg atcgacagcg tcggcaacct
     1561 ggtcggcatc aataccgcga tctattcgcg caccggcggc aaccacggca tcggtttcgc
     1621 gatcccggcc tccagcgccc gcagcatcat ggaacagatt atcgaaaacg gctcggtgac
     1681 gcgcggctgg atcggcgtcg aagcccagga aatcaccgcc gaactggccg agtccttcgg
     1741 cctgccgggc accgaaggtt cactgattgc cggcgtcgtg cgcggcagcc cggccgacaa
     1801 ggcaggcatc cgccccggcg acgtcctgct gtcgatcgac ggcaaaccgg tcggcgaccc
     1861 gcaggtaatg ctcgacctga tcgccgaact caagccggag agcagcgccg cttttcgcct
     1921 gcgccgcgac aagagcatcc tcgaattgca ggtcggcatc ggcaagcgtc cgcccttgcg
     1981 caacgcggag taagcaggat gtgccagttg ctcggcatga actgcaatgt gccgactgac
     2041 atctgctttt ccttttccgg tttccaggcg cgcggcggcg ccaccgacgt gcacgccgat
     2101 ggctggggca tcgccttctt cgagcagcgc ggcgcccgcc tcttcctcga tcctcagccc
     2161 tcggcccgtt cgccgatcgc cgaactggtc gcccgctacc cgatccgctc gcagaacgtg
     2221 atcgcccata tccgcaaggc gacgcaggga tcggtacggc tcgagaacac ccacccgttc
     2281 atgcgcgaac tttggggacg ctactggatc tttgcccaca acggcaatct gctcgatttt
     2341 tcaccgaagc tggacggcag cttcctgccg gtcggcgata ccgacagcga acgcgccttc
     2401 tgctggctgc tgcaggaatt ccggcgtagc ttcggcgaag ccccgccggc accggcagca
     2461 ctattcgaca gattgcgcga actgacgctg ctacttgccg cgcacggcga gtgcaatttc
     2521 ctgctctcca atggcgactg cctgttcgcc catgcctcga cgcggctcag ctacatcgtg
     2581 cgccaggcgc cgtttgccga agcccacctg aaagaccagg acctcacggt cgatttccgt
     2641 gaagtgacaa ccgagaagga tcgcgtcgcg gtaatcgcca cgctgccgct gaccgacaac
     2701 gaagcctgga cggagatgcc gcccggcagc ctgtggcgtt ttcacgatgg cgcggtggtc
     2761 gggcaatgcc cgacccggcc cggaccgcca cgtcgctgac gcttacttct cggtcagttt
     2821 gcggaattcc gcttcggcct gcgccatttc agcatcgagt tcagcttcga ccttggctgc
     2881 gccatcgtcc tcggcggcct ccggcgccgg cttggaaacc agacgcgccg ccagcagacc
     2941 cagttcgtaa agcaggcaca tcgggaccgc caacgccagt tgcgatacga cgtctggcgg
     3001 cgtgaccacg gcggcgacaa cgaaggcgcc gacgatgaaa taggaacgcc attccttgag
     3061 cttgtcgacg ctgacgacac gcagcttgac cagcacgacc aacgccactg gcacctcgaa
     3121 agcgaggccg aaggccagga acatggtcat cacgaaggac aggtaggctt cgatatccgg
     3181 cgccggcgtg atgctcttgg gcgcaaaacc ggcaatgaaa tggaagacct ggccgaagac
     3241 gaagtagtag cagaatgcca tgccgagcag gaacagcagc gtactcgaaa cgatcagcgg
     3301 aatacccagc cgccgctcat gttcgtacag cccgggcgag acgaagcccc aggcctggaa
     3361 aaggatgaac gggagggcaa cgacgaaagc caccatggcg gtgaccttga gcggcaccat
     3421 gaagggggtg acgacgccaa tcgccaccat cttggtacct tccg
//
LOCUS       DAOSDX010000214         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9603_length_3463_cov_3.691021, whole genome shotgun sequence.
ACCESSION   DAOSDX010000214 DAOSDX010000000
VERSION     DAOSDX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9603_length_3463_cov_3.691021"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /gene="aceF"
                     /locus_tag="PLN02_05910"
     CDS             <1..1434
                     /gene="aceF"
                     /locus_tag="PLN02_05910"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HOI51893.1"
                     /translation="LGAKVGEGALLIKVETGEAAAAAAPAPAAAPAPAAAAPVAAAPA
                     AGGGVVEVKVPDIGDFAEVPVIDLFVKVGDTIKVDDAICTLESDKATMDVPSSAAGVV
                     KEVLVQLGSKVSEGALLIKVETGAAAAAPQAAAAAPAAAPAPVAAAPAPAAAAPASLP
                     AAQPQSLALGAKVHASPSVRAYARELGVDLSKVAASGPKGRIVKEDLTKYVKGVMSGA
                     TAAPVAAAGASLGGGLDLLPWPKVDFSKFGEIEVKPLSRIKKISGQNLSRNWVMIPAV
                     TYHEDADITDLEAFRVLLNKENEKGGGAKLTMLAFLMKACVKGLQKFPEFNASLDGDN
                     LVLKKYFNIGFAADTPNGLVVPVVKNVDKKSVFELAQESGDLAKQARDGKLKPADMQG
                     ACFTISSLGGIGGTYFAPIVNAPEVAILGVNKSAMKPVWDGKQFVPRLTLPLSLTADH
                     RVIDGALATRFNVYIAQLLADFRRVAL"
     gene            1447..3219
                     /gene="lpdA"
                     /locus_tag="PLN02_05915"
     CDS             1447..3219
                     /gene="lpdA"
                     /locus_tag="PLN02_05915"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOI51894.1"
                     /translation="MSNLVEVKVPDIGDFDSVPVIDLFVKVGDTIKVDDAICTLESDK
                     ATMDVPSPVAGTVKEVLVQLGAKVSEGVLLIKVEAGAAAAAAPAPAAAAAPAAAPVAA
                     PTAAAHAGSADIECDMLVLGGGPGGYSAAFRAADLGLKTVIVERYSTLGGVCLNVGCI
                     PSKALLHVTAVMEETAHLADAGVSFAAPQIDVDKLRAHKEKVVGKLTGGLAGMAKGRK
                     VDIVRGVGTFLDPYHLAVEVTDGNGQDKTGAKKVVKFKQCIIAAGSAAVHLPFIPQDP
                     RIVDSTGALELRFVPKKMLVIGGGIIGLEMATVYSALGARIDVVEMADGLMQGPDRDA
                     VKVWEKLNAQRFERVMLKTKTVAVDAREDGLWVKFEGEGAPNADAVRYDMILQAAGRA
                     PNGKKIGADQAGVIVDERGFIPVDAQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAA
                     EVAAGQKSAFDVQVIPGVAYTHPEVAWVGYTEDQAKKEGRKVETAKFPWAASGRAIAN
                     GADYGFTKLIFDAESHRVIGGAIVGPSAGDMIGEVCLAIEMGCDAVDIGKTIHPHPTL
                     GESIGMAAEIAHGSCTDVPAPRKK"
     gene            complement(3248..>3463)
                     /locus_tag="PLN02_05920"
     CDS             complement(3248..>3463)
                     /locus_tag="PLN02_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51895.1"
                     /translation="AMRPANPLPFGFAVAGGRQRCSAREWNNHSLRLAPWRPSRHGKR
                     RPADYCEHALAGGQKKATDETVAKYER"
BASE COUNT          607 a         1152 c         1155 g          549 t
ORIGIN      
        1 ctcggcgcca aggttggtga aggcgcgctg ctgatcaagg tcgaaaccgg tgaggccgct
       61 gccgcagcgg cgcccgctcc ggctgctgcg cccgctccgg ccgcagccgc gccggttgcc
      121 gcagcgcccg ctgccggcgg cggcgtggtc gaagtgaagg ttccggacat cggcgatttc
      181 gccgaagtgc cggtgatcga cctcttcgtc aaggtcggcg acacgatcaa ggtcgatgac
      241 gccatctgca cgctggaatc cgacaaggca acgatggatg tgccgtcctc ggccgccggc
      301 gtggtcaagg aagtgctggt ccagctcggt tccaaggtca gcgaaggtgc actgctgatc
      361 aaggtggaaa ccggtgcggc cgccgctgcc ccgcaggctg ctgctgcggc gcccgccgcg
      421 gcaccggccc cggttgccgc cgcgcccgcg ccggccgctg ccgccccggc gtcgctgccg
      481 gcggcccagc cgcagtcgct ggcgctcggc gccaaggtcc atgcctcgcc gtcggtgcgc
      541 gcctatgccc gcgaactggg tgtcgatctg tccaaggtcg ccgcctccgg tcccaagggg
      601 cgcatcgtca aggaagacct gaccaagtac gtcaagggcg tgatgtccgg cgccaccgcg
      661 gcaccggtcg ctgccgccgg cgccagcctc ggtggcgggc tcgacctgct gccctggccg
      721 aaggtcgatt tcagcaagtt cggcgaaatc gaggtcaagc cgctgtcgcg catcaagaag
      781 atttccggcc agaacctgtc gcgcaactgg gtgatgatcc cggccgtcac ctatcacgaa
      841 gacgccgaca tcaccgacct ggaagccttc cgtgtcctgc tcaacaagga aaacgagaag
      901 ggtggcggcg ccaagctgac catgctggct ttcctgatga aggcttgcgt caagggtctg
      961 cagaagttcc cggaattcaa cgcttcgctc gacggcgaca acctggtgct gaagaagtat
     1021 ttcaacatcg gcttcgccgc cgacaccccg aacggcctgg tcgtgccggt ggtcaagaac
     1081 gtggacaaga agtcggtgtt cgaactggcg caggaatccg gtgacctggc caagcaggcg
     1141 cgtgacggca agctcaagcc ggccgacatg cagggggcct gcttcaccat ttcctcgctc
     1201 ggcggcatcg gcggcaccta cttcgcgccg attgtgaacg cgccggaagt ggctatcctc
     1261 ggcgtcaaca agtcggcgat gaagccggtc tgggacggca agcaattcgt accgcgcctg
     1321 accctgccgc tgtcgctgac cgccgaccac cgcgtcatcg acggcgcgct ggcgacccgc
     1381 ttcaacgtct atatcgcgca actgctggcc gacttccgtc gcgtcgcgct gtaaggggga
     1441 agaaccatga gcaatctggt tgaagtgaaa gtcccggaca tcggcgattt cgacagcgtg
     1501 ccggtgatcg acctgttcgt taaggtcggc gacacgatca aggtcgacga cgcgatctgc
     1561 acgctggaat cggacaaggc gacgatggat gtgccgtcgc cggtcgccgg cacggtcaag
     1621 gaagtgctgg tgcagctcgg cgccaaggtc agcgaaggcg ttctgctgat caaggtggaa
     1681 gccggtgctg cggcagccgc cgcacccgcg ccagccgcag ccgctgcccc ggcggcggcg
     1741 ccggtcgccg cgccgacggc ggccgcccat gccggcagcg ccgacatcga atgcgacatg
     1801 ctggtgctgg gcggcggccc cggcggctat tcggccgcct tccgcgccgc cgatctcggg
     1861 ctgaagacgg tgatcgtcga gcgttattcg acgctcggcg gcgtctgcct caacgtcggc
     1921 tgcattccgt cgaaggcgct gctgcacgtc accgccgtca tggaagaaac cgcccatctg
     1981 gctgacgccg gcgtcagctt cgccgccccg cagatcgacg tggacaagct gcgcgcccac
     2041 aaggaaaagg tggtcggcaa actgaccggc ggtctggccg gcatggccaa gggccgcaag
     2101 gtcgacatcg tgcgcggcgt cggcactttc cttgatcctt accatctggc cgtcgaagtc
     2161 accgacggca acggccagga caagaccggc gccaaaaagg tggtgaagtt caagcagtgc
     2221 atcatcgccg ccggttcggc ggccgtgcat ctacccttca tcccgcagga cccgcgcatc
     2281 gtcgattcga ccggcgcgct ggaactacgc ttcgtgccga agaagatgct ggtcatcggc
     2341 ggcggcatca tcggcctgga aatggccacg gtgtattcgg cgctgggcgc caggatcgac
     2401 gtcgtcgaga tggccgacgg cctgatgcag ggcccggacc gcgacgcggt caaggtctgg
     2461 gaaaagctca acgcccagcg tttcgagcgc gtcatgctga agacgaagac ggttgctgtc
     2521 gacgccaggg aagacggact gtgggtcaag tttgaaggcg aaggcgcccc gaacgccgac
     2581 gccgtgcgct acgacatgat cctgcaagcc gccggtcgcg cgccgaacgg taaaaagatc
     2641 ggcgccgacc aggccggcgt catcgtcgac gagcgcggtt tcatcccggt cgatgcgcag
     2701 atgcgcacca acgtgccgca catcttcgcc atcggcgaca tcgtcggcca gcccatgctg
     2761 gcgcacaagg cggtgcatga ggcgcacgtc gcggcggaag tcgccgccgg ccagaaatcg
     2821 gccttcgacg tccaggtcat ccccggcgtc gcctacaccc acccggaagt ggcgtgggtc
     2881 ggctataccg aggaccaggc gaagaaggaa gggcgcaagg tcgagaccgc caagttcccc
     2941 tgggcggcct ccggtcgtgc catcgccaac ggcgccgact acggcttcac caagctgatc
     3001 ttcgacgccg aatcgcaccg cgtcatcggc ggcgccatcg tcggcccgtc ggccggcgac
     3061 atgatcggcg aagtctgcct ggcgatcgag atgggctgcg acgcggtcga catcggcaag
     3121 accatccacc ctcaccccac ccttggcgaa tcgatcggca tggccgccga aatcgcccac
     3181 ggttcctgca cggacgtccc cgcgccgcgc aagaagtaac gatcctctgc gttacgctaa
     3241 cctctagcta gcgttcgtat ttggcgacgg tttcatccgt cgcctttttt tgtccaccgg
     3301 cgagggcgtg ttcacagtaa tctgcgggac ggcgtttgcc gtggcgggat gggcgccaag
     3361 gcgcgaggcg cagggaatgg ttgttccatt cccgagccga gcaacgctgg cgaccgcccg
     3421 ccacggcaaa cccgaagggc aaggggttgg cggggcgcat tgc
//
LOCUS       DAOSDX010000215         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9610_length_3462_cov_4.117405, whole genome shotgun sequence.
ACCESSION   DAOSDX010000215 DAOSDX010000000
VERSION     DAOSDX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9610_length_3462_cov_4.117405"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1222
                     /locus_tag="PLN02_05925"
     CDS             311..1222
                     /locus_tag="PLN02_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI51896.1"
                     /translation="MRKDLLSLPPLEPLRGFVAAASRRSITLAAADLCLTQSAISRQI
                     QALESALGVPLFVRKHRAIELTPAGERLLPAAIAWLAEYGEIAASLRQRAVSADSVTI
                     TASIGVTALWLLPRLGEFQAAHPAVEVRVVAANKPVDLAPAGMDLAIRYQTEPTIGRG
                     DRLLFRESIAPVLAPALAEKLAASPEESPTLIDYQERSLPGLDWTPWLASLGLSGRSL
                     RRLGFNQYDQAVQAAIAGQGVVLGRLPLLASALDDGRLCRWPALPAFVSPYGYWLLRA
                     PGSDGAAVAAFAGWLADLGGAGEAGGA"
     gene            complement(1238..>3462)
                     /locus_tag="PLN02_05930"
     CDS             complement(1238..>3462)
                     /locus_tag="PLN02_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HOI51897.1"
                     /translation="TIFVLIGGMLLSILPRLPSLDLVTDYRPKIPLRIYTADGALLGE
                     FGEERRDFTPIQEIPPLMKEALLAIEDARFYEHGGVDYHGVARAVAAGLSGGMRQGAS
                     TITMQVARNFFLTPEKTLRRKLTEVMLSYRIEAALSKDQILELYMNQIYLGQRSYGFS
                     SAARTYFRKRLPELTLAETAMLAGIPQNPAKHNPAVNPARAKARQELVLKRMLQLEKI
                     TQAQFDEAVAQPLAVSQSLQFEAHADYVAELVRQELFARYQGAAYTQGFNVYTTLDKA
                     EQNAAYESLRRNVLAYDQRHGYRGPEGFVELPADADEREEVIDQALARHPGSDKLIAA
                     VVTEVSAKRVRAEPAFGDTLEITGNGLRFAARALQPNAGPALRIRVGSVIRVSQDGKG
                     HWAIAQMPEVEAAFVALDAEDGSYRALVGGFDFYRRQFNHVTSAWRQPGSIIKPFVYS
                     AALEKGFSPATLMNDAPLSLPGGADGQPWEPSNDDGYDGPLTLRRALARSKNVVAVRL
                     LQAITPQYARDYLTHFGFDAAKHPDNLTLTLGSGSVTPLQMAQAYAVFANGGHTVNPR
                     LIEKITDARGQVLWEAPAPSKEERQPVIDPRNAFIIDSMLREVVSSGTGAAASQRLGR
                     SDLAGKTGTTSDAVDGWFAGYAGKVVGVAWMGYDQPKSLGAREFGATLALPIWADYMR
                     QALAGKPITERTAPSGVSYVDGDWVYDEFAGDAGVKALDVEVSPIDSILDAMRKLFGE
                     "
BASE COUNT          498 a         1194 c         1190 g          580 t
ORIGIN      
        1 ggcgggtgat ccaggctcgg ccgtcctcca cgttcagcgc cgcgactcgg cgcggcgaca
       61 gtcgggccag ttgctcgggg cccagttgca ggcggcagaa caggctgccg ggcaggtcgg
      121 ggtgttcggt catgctcgtc tcctgcgccg ggcttgacga aagatcgcca aaagcccagc
      181 atccagatgc gtttcggttg caaatcccgc atcggttgaa gcttcgagtc cgctgcggtc
      241 gaagccagtt ttccccagaa ttgaggccgg ttcaaacgca atatcggcat tgagtggatg
      301 cgtgaaaatc atgcgaaaag acctgctttc gctgccgccg ctggagcctc tgcgcggttt
      361 tgtcgcggcg gccagccggc gcagcatcac gctggcggcg gccgatctgt gcctgaccca
      421 gtcggcgatc agccgccaga tccaggcgct ggaaagcgcg ctcggcgtac ccttgttcgt
      481 ccgcaagcac cgcgccatcg aactgacccc ggccggcgag cgcctgttgc cggccgccat
      541 cgcctggctc gccgaatacg gcgagatcgc cgccagcctg cgccagcgcg ccgtcagcgc
      601 cgacagcgtg acgatcaccg ccagtatcgg cgtcaccgcg ctctggctgc tgccgcgcct
      661 gggcgaattc caggccgccc acccagccgt cgaagtccgc gtcgtcgccg ccaacaagcc
      721 ggtcgacctg gccccggccg gcatggatct ggccatccgc taccagaccg aaccgaccat
      781 cggccgcggc gaccgcctgc tgttccgcga gagcatcgcc ccggtgctgg cgccggctct
      841 ggcagaaaag ctcgcggcca gcccggaaga aagcccgacc ttgatcgact atcaggaaag
      901 aagcctgccc ggtctcgact ggacgccctg gctggccagt ctcggcctgt cggggcggag
      961 cttgcgccgg ctcggcttca accagtacga ccaggcggtg caggcggcca tcgccggcca
     1021 gggcgtcgtc ctcggccgtc tgccgctgct cgccagcgcg ctggacgacg gccgcctgtg
     1081 ccgctggccg gcgctgccgg ccttcgtcag cccctacggc tactggctgc tgcgcgcgcc
     1141 cggcagcgac ggcgcggcgg tcgctgcctt cgccggttgg ctggccgatt tgggtggggc
     1201 gggggaagcg ggcggggcct agccggcgcc ctccgcctca ctcgccgaac agcttgcgca
     1261 tcgcgtccag gatgctgtcg atgggcgaga cttcgacgtc cagcgccttg accccggcgt
     1321 cgccggcgaa ttcgtcgtac acccagtcgc catcgacgta ggagacgccc gatggcgccg
     1381 tccgctcggt gatgggcttg ccggccagcg cctgacgcat gtagtcggcc cagatcggca
     1441 gcgccagggt ggcgccgaat tcgcgggcgc cgagtgattt cggctggtcg tagcccatcc
     1501 acgccacccc taccaccttg ccggcgtagc cggcgaacca gccgtcgacc gcgtcgctgg
     1561 tggtgccggt cttgccggcc aggtcgctgc gccccaggcg ctggctggcg gcggcgccgg
     1621 tgccgctgct caccacctcg cgcagcatgc tgtcgatgat gaaggcgttg cgcggatcga
     1681 tcaccggctg cctctcttcc ttcgacggcg ccggggcttc ccacagcacc tggccgcggg
     1741 cgtcggtgat cttctcgatc aggcgcgggt tgaccgtgtg gcccccgttg gcgaacaccg
     1801 cgtaagcctg ggccatctgc agtggcgtca ccgagccgct gcccagcgtc agcgtcaggt
     1861 tgtccgggtg tttggcggcg tcgaaaccga agtgcgtcag gtagtcgcgc gcatactgtg
     1921 gcgtgatcgc ctgcagcagg cgcacggcga ccacgttctt cgagcgcgcc agcgcccggc
     1981 gcagtgtcag cgggccgtcg tagccatcgt cgttgcttgg ctcccacggc tggccgtcgg
     2041 cgccgccggg cagcgacagc ggcgcgtcgt tcatcagggt ggccggcgag aagccttttt
     2101 ccagcgccgc cgaatagacg aagggcttga tgatcgatcc cggctggcgc caggcgctgg
     2161 tgacgtggtt gaactgccgg cggtagaaat cgaaaccgcc gaccagcgcc cggtaggaac
     2221 cgtcctcggc gtcgagggcg acgaaagccg cctccacctc cggcatctgg gcaatcgccc
     2281 agtggccctt gccgtcctgg ctgacgcgga tcaccgaacc gacgcggata cgcagcgccg
     2341 gtccggcgtt cggctgcagc gcgcgggcgg cgaagcgcag gccgttgccg gtgatctcca
     2401 gggtgtcgcc gaaggccggt tcggcgcgca cgcgcttggc cgaaacctcg gtgacgacgg
     2461 cggcgatcag tttgtcgctg cccgggtgtc tggccagcgc ctggtcgatc acttcctcgc
     2521 gctcgtcggc gtcggccggc agttcgacga aaccctccgg gccgcggtag ccatggcgct
     2581 ggtcgtaagc cagcacgttg cgccgcagcg actcgtaggc ggcgttctgc tcggccttgt
     2641 cgagcgtggt gtagacattg aagccctggg tataggcggc gccctggtaa cgcgcaaaaa
     2701 gctcctggcg taccagttcg gcgacgtagt cggcatgcgc ttcgaactgc aggctctggc
     2761 tgaccgccag cggctgggcg acggcctcgt cgaattgcgc ctgggtgatc ttctccagtt
     2821 gcagcatgcg cttcaggacc agttcctggc gcgccttggc gcgtgccgga ttgaccgccg
     2881 ggttgtgctt ggccgggttc tgcggaatgc cggcgagcat cgccgtctcg gccagcgtca
     2941 gctcgggcag gcgcttgcgg aaataggtgc gggcggcgct ggaaaagccg taggagcgct
     3001 ggccgaggta gatctggttc atgtacagct cgaggatctg gtccttgctc aaggccgctt
     3061 cgatgcggta ggacagcatc acctcggtca gcttgcggcg cagcgtcttc tccggcgtca
     3121 ggaagaaatt gcgcgccacc tgcatggtga tcgtcgacgc gccctggcgc atgccaccgc
     3181 tcagtccggc cgccaccgcc cgcgccacgc cgtggtaatc gaccccgccg tgctcgtaga
     3241 aacgcgcatc ctcgatcgcc agcagcgcct ccttcatcag cggcggaatc tcctggatcg
     3301 gcgtgaagtc gcggcgctcc tcgccgaact ccccgagcag cgcgccgtcg gccgtgtaga
     3361 tgcgcagcgg gatcttcggc cggtagtcgg tcaccagatc gagcgacggc aagcggggca
     3421 ggatgctcag cagcatgccg ccgatcagca cgaatatcgt ta
//
LOCUS       DAOSDX010000216         3457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9627_length_3457_cov_2.534979, whole genome shotgun sequence.
ACCESSION   DAOSDX010000216 DAOSDX010000000
VERSION     DAOSDX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9627_length_3457_cov_2.534979"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PLN02_05935"
     CDS             complement(<1..1282)
                     /locus_tag="PLN02_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51898.1"
                     /translation="MTYVAPATLPEVLSHLEFEEGLPADDPRYVDTLDARNSHDLFKH
                     LSRKFGLWKDQVIAPTQKHLLFFGHLGSGKTTELRHYAKKFNDTGRFCTLEVNINAEL
                     DRSNCQFPDLLMAMARTLLADLQERGIVIGDAQLQPLYDWFRSTELTRLSEHALNAEV
                     KTGAEAQLSVPLLCKLFASVTTSFRTGATYRSEIRDKIRNSYGQLAGAFNNFLRFAEQ
                     SLKTKSGIDRVLFVLDGTDKLRDDDSRRLFDTDVAQMLQVDALILYTAPLHLAYESNI
                     GGRLDHFFLPMIKINERNGSECDAGRRALRDLLLRRADRKLFADDETLRTLIEHCGGH
                     PRELLRLLKYCCEFADEGIDRAVAGKAIDALAGEYRRFLDPEDYALLVAKDADQQHGG
                     NDERSKKLLYNLALLEYNGGSWQRSHPVVRTLEGY"
     gene            complement(1415..2254)
                     /locus_tag="PLN02_05940"
     CDS             complement(1415..2254)
                     /locus_tag="PLN02_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51899.1"
                     /translation="MSAAENYVQELTLATELLPLSPAEEAGFEPVAVATPPDEASDQA
                     SLLEFGMNLMERRQPKPPRKPPPAVDANALLSFVVGVTPQEREDALYSVQLASRSASA
                     LFDRFKDTEAWYGKYIETLEKLGWTSEQFAFSKYEQAEGELRMDQAALAVIAAIATQN
                     QLAVLNQSLDALKALAEEDGAIRLFDFHTTAEASGNFQIGAVQKSEDGVISMALGAFH
                     FTSDDQRRRFLFLSWGERSVQLWTAAQKMTFNAKHYARLRDAVEDKLGDAAELYIAEL
                     EIA"
     gene            2532..>3457
                     /locus_tag="PLN02_05945"
     CDS             2532..>3457
                     /locus_tag="PLN02_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51900.1"
                     /translation="MTFTPKRLVAALVSCGLLSLLHGPAMAGVDGLDYNLPTNIGSAV
                     MGLSGDGNVAVGTVRLDAFNYRAYKFSPGSNTQLDGPAGYEQVVAQAASTDGSVVAGW
                     ALPTYSSPSARALVWNGATMTQLPDLASGPGTIASQAWAIAGDGRKVAGFSYVNGAYH
                     AARWLDGGAGWSVQDLNGGAFTSSFGRGIDASGDTVVGYGSTMVLGYQGSNLVHISGL
                     VEEAFVWTAGGGMQGLGVLASDNDPVVGTMTPSSRAYAVSADGSTVVGISRGVGVNDI
                     PEAFRWTAAGGMLGLGTLNGGTYSIANGVNADG"
BASE COUNT          571 a          992 c         1196 g          698 t
ORIGIN      
        1 ggtagccttc cagggtacgc acgacggggt ggctacgttg ccagctaccg ccgttgtatt
       61 cgagcagcgc caggttgtag agcagtttct tgctgcgctc gtcgttaccg ccgtgctgct
      121 gatcggcatc cttggcgacg agcagtgcat agtcctcggg gtcgaggaag cggcgatatt
      181 cgccggccag ggcatcaatg gctttgccgg caacggcgcg gtcgatgcct tcgtcggcga
      241 attcgcagca gtatttgagc aggcgcaaga gttcgcgcgg gtggccacca caatgttcga
      301 tcagggtgcg cagcgtctcg tcgtcggcaa acagcttgcg gtcggcgcgt cgcaagagca
      361 ggtcgcgtag ggctcggcgc ccggcgtcgc actcgctgcc gttgcgctcg ttgatcttga
      421 tcatcggcag gaaaaaatga tcgagacgac cgccgatatt gctctcgtag gccagatgca
      481 gcggcgcggt gtacaggatc agggcgtcga cctgtagcat ttgcgcgaca tcggtgtcga
      541 acaatcggcg cgaatcgtcg tcgcgtaact tgtcggtacc atcgagtacg aacaggacac
      601 ggtctatgcc gcttttggtt ttgagggact gttcggcaaa acgcaggaaa ttattgaaag
      661 ccccggccag ttgaccgtag gaattgcgga tcttgtcgcg gatttcgctg cggtaggtcg
      721 cgccagtgcg gaaagatgtg gtgacggaag cgaagagctt gcacagcagc ggtacggaaa
      781 gctgtgcttc cgccccggtt ttgacttcgg cattgagggc gtgctcgctc aaacgggtta
      841 gctccgtgct gcggaaccag tcgtagagcg gctgcaactg ggcatcgcca atgacgatgc
      901 ccctttcctg caaatcggcg agcagggtgc gggccatggc catgagcagg tcgggaaact
      961 ggcaattgct gcggtccagc tcggcgttga tgttgacttc cagcgtgcag aagcgcccgg
     1021 tgtcgttgaa tttcttggcg tagtgccgca attccgtggt tttgccgctc cccaggtggc
     1081 cgaagaaaag caggtgcttt tgcgtggggg caatgacctg atctttccac aggccgaact
     1141 tgcgcgacag atgcttgaaa agatcgtgac tgttgcgcgc atccagcgtg tcgacgtaac
     1201 gcggatcgtc ggcaggcagt ccttcctcga attcgaggtg gctgagtacc tcgggaagtg
     1261 tggcgggtgc gacgtaggtc atgtctgggc tgtcgtgttg gagcgtggga agtctaacgc
     1321 gtccttggcc tcgggcaaag cgatggggcg tgccttttct tgtcccggtc cgatttcgcc
     1381 agcctccggg tagagagagg ctggcgatgt tcgctcaggc gatttccagc tccgcgatgt
     1441 agagctcggc ggcgtctccg agcttgtctt ccaccgcatc gcgcaggcgg gcgtagtgct
     1501 tggcgttgaa ggtcatcttc tgcgcggcgg tccacaattg caccgagcgt tcgccccagg
     1561 agagaaacag gaagcggcgg cgctggtcgt cggaggtgaa gtggaaggcg ccgagcgcca
     1621 tcgagatgac gccgtcttcc gatttctgca cggcgccgat ctggaaattg ccgctcgcct
     1681 cggcggtggt gtggaagtcg aacaggcgga tcgcgccgtc ttcctcggcc agcgccttga
     1741 gcgcgtcgag cgactggttg agcacggcga gctggttctg cgtggcgatg gcggcgatca
     1801 cggcgagcgc ggcctggtcc atgcgcagtt cgccctcggc ctgctcgtac ttgctgaagg
     1861 cgaactgttc gctggtccag ccgagttttt ccagggtctc gatgtacttg ccgtaccagg
     1921 cttcggtgtc cttgaaacgg tcgaacaggg cgctggcgct gcgcgaggcg agctggacgg
     1981 aatacagcgc atcttcgcgt tcctgcgggg tgacgccgac gacgaaggag agcagggcgt
     2041 tggcgtcgac ggccgggggc ggcttgcgcg gcggcttggg ctggcggcgt tccatcaggt
     2101 tcatgccgaa ttcgaggagc gaggcctggt cgctggcctc gtccgggggc gtcgcgacgg
     2161 ctaccggctc aaatccggct tcttccgctg gcgacagcgg cagcaattcg gtggcgaggg
     2221 tgagttcctg aacgtaattt tctgctgccg acactttcga tctccggtta tgcaaaacga
     2281 tatacatcaa gtatgccgta gtgctagtgg tttgtcgtgc gagggacttt tacaggaacg
     2341 tgaagatacc ccgggggcgg aagggcaatc ctgcattttg ctgatcgcag cgccagctcg
     2401 gccggccagg cctggtgctt gccggcgcca gaaaatgccg cctccccccg cgtttgcggg
     2461 gtagtggcca agggggcttc tgaagatact tgccagggtt tacaacaatt tacatatttc
     2521 aggggttggc aatgacattt acgccgaaac gtctggtcgc cgcgctggtc tcttgcggtc
     2581 tgctgagttt gctgcacggg ccggccatgg ccggtgttga cgggctggat tacaacctgc
     2641 cgaccaacat cggcagcgca gtgatggggt tgtccggcga cggcaatgtc gcggtcggca
     2701 cggtgcgtct cgatgcgttc aattaccgcg cctacaagtt ctcgccgggc agcaacacgc
     2761 agctcgacgg tccggccggt tacgagcagg tggtcgccca ggcggcgtct accgacggct
     2821 cggtcgtcgc cggctgggcc ttgcccacct acagcagtcc aagcgcgcgg gcgctggtct
     2881 ggaacggcgc caccatgacc cagttgccgg acctggcgag cgggccgggc accatcgcca
     2941 gccaggcctg ggcgattgcc ggcgacggcc gcaaggtggc gggcttcagc tatgtcaacg
     3001 gcgcctacca cgccgcgcgc tggctcgacg gcggcgccgg ctggtcggtc caggatctca
     3061 acggcggcgc cttcaccagc agtttcggtc gcggcatcga tgccagcggc gatacggtcg
     3121 tcggttacgg cagcacgatg gttctcggct accagggcag caacctcgtc catatcagcg
     3181 gtctggtcga agaggccttc gtgtggacgg cgggcggtgg catgcagggc ctgggcgtgc
     3241 tcgcttccga caacgatccg gtggtcggca cgatgacccc gagcagccgc gcctacgcgg
     3301 tgtcggccga cggcagcacg gtggtcggca tttcgcgcgg tgtcggcgtc aacgatatcc
     3361 ctgaggcttt ccggtggacc gctgcgggcg gcatgctcgg cctcggcacc ctgaatggcg
     3421 gtacctacag catcgccaac ggcgtcaatg ccgacgg
//
LOCUS       DAOSDX010000217         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9643_length_3452_cov_3.373565, whole genome shotgun sequence.
ACCESSION   DAOSDX010000217 DAOSDX010000000
VERSION     DAOSDX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9643_length_3452_cov_3.373565"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLN02_05950"
     CDS             complement(<1..256)
                     /locus_tag="PLN02_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI51901.1"
                     /translation="MNISAAAEGFSAEPKTLVESAYRALRRDIIEGTLAPGEKLRVEH
                     LKDNYGVGAGTLREALSLLIADALVVSQGQRGFRVSPVSLE"
     gene            428..1354
                     /locus_tag="PLN02_05955"
     CDS             428..1354
                     /locus_tag="PLN02_05955"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HOI51902.1"
                     /translation="MAMTGVLRPGHIQLKVLDLDESVEFYSNVLGLTETGRDAQGRVY
                     FKAWDERDHHSVILRQADSAGIDFFAFKVDSCATLDKLEKDLNAYGVATRRIPAGELL
                     ETGERVRFTLPTGHDIELYSQKSEVGNGMSDVNPEAWTAAAEKGIAPIRLDHCLLYGP
                     DIEKAQAIFTDVLGFYLVEHIALDDSQDLAIWLSCSIKAHDIAFVRHPEPGKLHHVSF
                     LLESWEKVLRAADIMSMNRVSIDIGPTRHGVTRGTTIYAFDPNGNRFETFCGGYQCYP
                     DNKPTKWTMDEVGAAVFYHDRKLNDRFLTVVS"
     gene            1354..1782
                     /locus_tag="PLN02_05960"
     CDS             1354..1782
                     /locus_tag="PLN02_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOI51903.1"
                     /translation="MQVAASTPGITAEAAAAACSAAVAHAAARGWRINVAVVDRGGNL
                     MAFLRQPGAFIHSIQIAIDKAYTSASFGFPTKAWMGAIGHDDGMKLGFANQPRLIVFG
                     GGLPVPGDGGEWLGGIGVSGASEAEDEECARAGLAAIGLA"
     gene            1813..3267
                     /locus_tag="PLN02_05965"
     CDS             1813..3267
                     /locus_tag="PLN02_05965"
                     /EC_number="1.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconic semialdehyde dehydrogenase"
                     /protein_id="HOI51904.1"
                     /translation="MKQILNFINGEFVATGKQFQKRSPLDNSLIGMVHEAGKAEVDAA
                     VRAAREALDGPWGRMTVVERTDILNRVADEITRRFNEFLDAECADTGKPKSLASHIDI
                     PRGAANFKIFADVIKNAPTEFFEMATPDGKGAINYAMRRPVGVVGVVCPWNLPLLLMT
                     WKVGPALACGNTVVVKPSEETPSTATLLGEVMNACGVPKGVYNVVHGFGPDSAGEFLT
                     TNQGVNAITFTGETRTGAAIMKAAADGARPVSLEMGGKNPAIVFADANLDVAIEATIR
                     SCFSNCGQVCLGTERVYVERPLFDSFVARLKEGAEKLKLGVPGDASANMGPLISQEHR
                     DKVLSYYRKAVDEGATVVTGGGVPEMPGELVNGAWVQPTIWTGIDENAAVVREEIFGP
                     CTTVMPFDSEEEVIKRANGTDYGLAASVFTQDVNRAHRVAGRIEAGLVWVNSWFLRDL
                     RTPFGGSKNSGIGREGGVHSLEFYTELKNVCIKL"
BASE COUNT          666 a         1152 c         1078 g          556 t
ORIGIN      
        1 cctcgagcga caccggcgag acgcggaaac cgcgctggcc ctggctgacc accaaggcat
       61 cggcgatgag cagggaaagc gcttcgcgca gggtgccggc gccgaccccg tagttgtcct
      121 tgaggtgctc gacgcgcagc ttctcgccgg gcgccagcgt gccttcgatg atgtcccggc
      181 gcaaggcgcg gtaggccgat tcgaccaggg ttttgggttc ggcggagaag ccttcggcag
      241 cagcgctgat gttcatggag atggcgtcga aacaaggatg aaaaattcta acccggagcg
      301 aaaattttcg ggttaattta aaaatctcga gaaaacgtat tgacatcgag attgcttgta
      361 aatatcgtaa tagtaaatcg gttgcaggat tgatcgatca atcacatcaa taaagaggag
      421 cagacaaatg gcaatgacag gcgtacttcg tcccgggcac atccagctca aggtgctcga
      481 tctcgatgag agcgtggagt tttactcgaa cgtgctcggc ctcacggaaa ccggccgcga
      541 cgcgcagggg cgcgtctatt tcaaggcctg ggacgagcgc gatcatcaca gcgtgatcct
      601 gcgtcaggcc gacagcgccg gcatcgactt ctttgccttc aaggtcgata gctgcgcaac
      661 gctcgacaag ctggaaaagg acctcaacgc ctacggcgtc gccacccgcc gcatcccggc
      721 cggcgaactg ctggaaaccg gcgagcgcgt gcgcttcacg ctgcccaccg gccacgacat
      781 cgagctttat tcgcagaaga gcgaagtcgg caacggcatg agcgacgtca atccggaagc
      841 ctggaccgcc gccgccgaaa agggcatcgc cccgatccgc ctcgaccatt gcctgctcta
      901 cggtccggac atcgagaagg cgcaggccat cttcaccgac gtgctcggtt tctacctggt
      961 cgagcacatc gcgctcgacg acagccagga cctggcgatc tggctgtcct gctcgatcaa
     1021 ggcgcacgac atcgctttcg tccgccatcc cgagccgggc aagctgcacc acgtctcctt
     1081 cctgctcgaa agctgggaaa aggtgctgcg cgccgccgac atcatgtcga tgaaccgcgt
     1141 ctcgatcgac atcggcccga cccgtcacgg cgtcacccgc ggcacgacga tctacgcctt
     1201 tgaccccaac ggcaaccgct tcgaaacctt ctgcggcggt taccagtgct acccggacaa
     1261 caaaccgacc aaatggacga tggacgaagt cggcgccgcc gtcttctatc acgaccgcaa
     1321 gctcaacgac cgcttcctga cggtggtcag ctgatgcaag tcgccgccag caccccgggc
     1381 atcaccgccg aggcggcggc agccgcctgc agcgcggcgg tggcgcatgc cgccgcacgc
     1441 ggctggcgga tcaacgtcgc cgtcgtcgac cgcggcggca acctgatggc cttcctgcgc
     1501 cagccgggcg ccttcatcca ctcgatccag atcgccatcg acaaggccta cacctcggcc
     1561 agcttcggct ttccgaccaa ggcctggatg ggggcgatcg gccacgacga cgggatgaag
     1621 ctcggcttcg ccaaccagcc gcgcctgatc gtcttcggcg gcggcctgcc ggtgcccggc
     1681 gacggcggcg agtggctcgg cggcatcggc gtctccggcg cttccgaggc cgaggacgag
     1741 gaatgcgcca gggctggcct ggccgccatc ggcctcgcct gacaacaaga attcaacaag
     1801 gaccaatagg gaatgaaaca gatactgaac ttcatcaacg gcgaattcgt cgccactggc
     1861 aaacagttcc agaagcgctc gccgctcgac aacagcctga tcggcatggt gcacgaggcc
     1921 ggcaaggccg aggtcgatgc cgccgtgcgc gccgcccgcg aagcccttga tgggccgtgg
     1981 gggcggatga ccgtggtcga gcgcaccgat atcctgaacc gcgttgccga cgagatcacc
     2041 cgtcgcttca acgaattcct cgacgccgag tgcgccgata ccggcaagcc gaagagcctc
     2101 gcctcgcata tcgacatccc gcgcggcgcc gccaacttca agatcttcgc cgacgtgatc
     2161 aagaacgcgc cgaccgagtt cttcgaaatg gcgacgccgg acggcaaggg tgccatcaac
     2221 tacgcgatgc gccggccggt cggtgtggtc ggtgttgtct gcccgtggaa cctgccgctg
     2281 ctgctgatga cctggaaggt cggcccggcg ctcgcctgcg gcaacacggt ggtggtcaag
     2341 ccgtcggaag aaaccccgtc cacggcgacc ctgctcggcg aagtgatgaa cgcctgcggc
     2401 gtgccgaagg gcgtctataa cgtcgtgcat ggtttcggtc ccgattcggc cggcgaattc
     2461 ctgaccacca accagggcgt caacgccatc accttcaccg gcgagacgcg caccggtgcc
     2521 gccatcatga aggccgccgc cgacggcgcc cggccggtgt cgctggaaat gggcggcaag
     2581 aatccggcca tcgtcttcgc cgacgccaac ctcgacgtcg ccatcgaagc caccattcgt
     2641 tcctgcttct ccaactgtgg ccaggtctgc ctcggcaccg agcgcgtcta tgtcgaacgc
     2701 ccgctgttcg acagcttcgt cgctcgcctc aaggaaggcg ccgagaagct caagctcggc
     2761 gtgccgggcg atgcttccgc caacatgggg ccgctgatca gccaggagca ccgcgacaag
     2821 gtgctctcct actaccggaa ggcggtggac gagggtgcca ccgtcgtcac cggcggtggc
     2881 gtgcccgaga tgccgggcga actggtcaac ggtgcctggg tgcagccgac catctggacc
     2941 ggcatcgacg agaacgccgc agtggtgcgc gaggaaatct tcgggccctg tacgacggtg
     3001 atgccgttcg acagcgaaga ggaagtcatc aagcgcgcca acggcaccga ctacgggctg
     3061 gccgcctcgg tgttcaccca ggacgtcaat cgcgcccacc gcgtcgccgg gcgcatcgaa
     3121 gccggtctgg tctgggtcaa cagctggttc ctgcgcgact tgcgcacgcc gttcggcggc
     3181 tcgaaaaact ccggcatcgg ccgcgagggc ggcgtgcatt cgctcgagtt ctacaccgaa
     3241 ctcaagaacg tctgcatcaa gctgtaaggc gcatcggcat gtccgccaac tctcccgaaa
     3301 tcggccagcg catcgtcgct gccggcatcg cgaccaacta ccacgatgtg gggagcgggg
     3361 cgccggtgct gctgatccac ggctccggcc ccggcgtcac cgcctgggcc aactggcggc
     3421 tggtgctgcc ggaactgggc aagacctgcc gg
//
LOCUS       DAOSDX010000218         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9648_length_3451_cov_2.746761, whole genome shotgun sequence.
ACCESSION   DAOSDX010000218 DAOSDX010000000
VERSION     DAOSDX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9648_length_3451_cov_2.746761"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLN02_05970"
     CDS             complement(<1..555)
                     /locus_tag="PLN02_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI51905.1"
                     /translation="MNCRPTPIAACLALAFAVPASAEDAVRLDPLVITGSRVEQNTFD
                     LPAAIDVVDVASVGATQARVNASEALAGVPGIVVQNRQNYAQDLQISSRGFGARTAFG
                     VRGIRLVADGIPASMPDGQGQAATFNLDRAERIEVMRGPLSAIYGNAAGGVIQLFTPD
                     GKGRPSVEGRFSAGSDSSWKTDLSS"
     gene            complement(688..1335)
                     /locus_tag="PLN02_05975"
     CDS             complement(688..1335)
                     /locus_tag="PLN02_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI51906.1"
                     /translation="MNPSLKNVRVVLADDHAVVRLGFRLLLEGAGALVVGEAADGEGA
                     LRLHAEMAPDLLVMDVSMPGGGGLLALERLRARDPVARILMLSAHHDEIVPVRALRLG
                     ANGYLCKRAAPAEFLRAVAQVAAGRRYLDPELAQQVALAQLSGAAQPLETLTEKELAV
                     FLKLAAGQTVNDVAGDFHVSPSTVGTHLYHIKQKLNLRNPAEITLLAVRNGLIEA"
     gene            complement(1332..2666)
                     /locus_tag="PLN02_05980"
     CDS             complement(1332..2666)
                     /locus_tag="PLN02_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOI51907.1"
                     /translation="MRPLRLHTRISLVLTGLAGSLCAVLVGLWLHGARQAIHEEIEAA
                     NRVSGQWLAALVEHLPANAADLQAALAPLGRIRANELQLLDAGGRLHYRSPPPTYKAG
                     RQAPDWFAKLLRPQIAPRVLAVGELRLVLVPDASRAVIDAWDELCAVAGWALGLLGLL
                     FVVVRRALDRALQPLDEVMRALQRIGYGRFDLRLPVFPVPELARLSNAFNGMADRLRA
                     AVDDNVRLETERELAQRLQARLEEERRQIARELHDEMAQGITAVRALAGAVVQRTAEQ
                     PALQAPAQHILAVTGELQDGVRRILHRLRPLPGGELAVRLNDYCASWRRQHETVALQL
                     RVALAGHEPDDALAAAALRIVQEGLTNVARHAAASEAELSVSGGDGVLRIALLDNGRG
                     AGGRSAAAGCGLGLTGMGERVAGLGGSLHFFTPAGGGFGLVATLPLFSLEEA"
     gene            complement(2672..>3451)
                     /locus_tag="PLN02_05985"
     CDS             complement(2672..>3451)
                     /locus_tag="PLN02_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI51908.1"
                     /translation="ARTLEAGLRGKLAGGIGWNAGVFRTVADDDILFVGTSTSAGYFT
                     NFGRTRRQGVELGIDGRHGKFDWFASYSWLHATFGSAACLLAENNSSRGTSAACTANG
                     QDDEIEVRKGDRLPGLPAHSLKLGLSWQAADWLRLGGDLQAFSGQYARGNENNAHRAG
                     SATDAFGNTRVFSGSGRLPGYAILNLNAEARLGGGWEVFARVDNVFDKRYATAAALAE
                     NPFSANGAFQVDSADWRRETFVAPGAPRAGWLGVRYRFAGL"
BASE COUNT          486 a         1227 c         1258 g          480 t
ORIGIN      
        1 actcgacaag tcggttttcc agctgctgtc gctgccggcg ctgaagcgcc cctcgacgct
       61 cggccggcct ttgccgtccg gcgtgaacaa ctggatgacg ccacccgccg cgttgccgta
      121 gatcgccgac aagggaccgc gcatgacctc gatgcgctcg gcgcggtcga ggttgaaagt
      181 cgccgcctgg ccttgcccgt ccggcatcga cgccgggatg ccgtcggcga ccagacggat
      241 gccgcgcacc ccgaaggcgg tacgggcgcc gaagccgcgc gacgaaatct gcaggtcctg
      301 ggcgtagttc tgacggttct ggacgacgat ccccggcacc ccggccagcg cttccgaagc
      361 attgacccgg gcctgcgtcg caccgacgct cgccacatcg acgacgtcga tcgccgccgg
      421 cagatcgaaa gtattctgct cgacgcggct gccggtgatg accagcgggt cgagccgcac
      481 ggcgtcttcg gcggacgccg gcacggcgaa ggcgagcgca aggcaggcgg cgatgggggt
      541 tgggcggcag ttcatcagtc tctctcctgg tttatggttg ggcggaccgt ggcggcccgt
      601 ctccggttca ttattcccgg ccgccgccgg cctgccgtcc cggagttcgc cagcgcggcc
      661 tcatgaaaat catgattcgc ccccgtctca ggcttcgatc agcccgttgc gcaccgccag
      721 cagcgtgatc tccgccgggt tgcgcaggtt cagcttctgc ttgatgtggt agaggtgggt
      781 gccgaccgtg ctcgggctga cgtggaagtc gccggccacg tcgttgaccg tctgcccggc
      841 ggcgagtttg aggaagaccg ccagttcctt ctcggtcagc gtctccagcg gctgcgcggc
      901 gccggagagc tgggccagcg ccacctgctg cgccagctcg ggatcgaggt agcgccgccc
      961 ggccgccacc tgggcgacgg cgcgcaggaa ctcggccggc gccgcccgct tgcacaggta
     1021 gccgttggcg cccaggcgca aggcgcgcac cggcacgatc tcgtcgtgat gcgccgagag
     1081 catcaggatg cgcgccaccg ggtcgcgggc gcgcaggcgt tcgagcgcca acaggccgcc
     1141 gccgcccggc atcgacacat ccatcaccag caggtcgggg gccatttcgg cgtgcaggcg
     1201 cagcgcgcct tcgccgtctg ccgcctcgcc gaccaccagc gctccggccc cttcgagcaa
     1261 gagccggaaa ccgaggcgga ccaccgcgtg atcgtcggcg agcacgacgc ggacgttttt
     1321 cagggatggg ttcatgcttc ctccagggaa aaaagcggca gcgtcgcgac caggccgaag
     1381 ccgccgccgg ccggggtgaa gaaatgcagg ctgccgccga ggccggccac ccgctcgccc
     1441 atgccggtca ggccgaggcc gcagccggcg gcggcggagc gcccgccggc gccgcgcccg
     1501 ttgtcgagca gcgcgatacg caggacgccg tcgccgccgc tgacgctcag ctcggcctcg
     1561 ctggcggcgg cgtgccgggc gacgttggtc agcccttcct ggacgatgcg cagcgccgcc
     1621 gcggccagcg catcgtccgg ctcatggccg gcgagcgcga cgcgcagttg cagcgccacc
     1681 gtctcgtgct ggcgccgcca actggcgcaa tagtcgttca gccgcaccgc cagctcgccg
     1741 ccgggcagcg gccgcagacg gtgcaggatg cggcggacgc cgtcctgcag ttcgccggtc
     1801 accgccagga tgtgctgcgc cggtgcctgc agcgccggct gctccgccgt gcgctggacg
     1861 acggcgccgg ccagcgcgcg cacggcggtg atgccctgcg ccatctcgtc gtgcagttcg
     1921 cgggcgatct gccgccgctc ctcctccagc cgcgcctgca ggcgctgcgc cagctcgcgc
     1981 tcggtttcca ggcggacgtt gtcgtcaacc gccgcccgca ggcggtcggc catgccgttg
     2041 aaggcattcg acaggcgggc cagttcgggc accggaaaga ccggcagacg caggtcgaag
     2101 cgcccgtaac cgatccgctg cagcgcccgc atcacctcgt ccagcggttg caaggcgcgg
     2161 tccagcgcgc ggcggacgac gacgaagagc aagccgagca ggccgagcgc ccagccggcg
     2221 acggcgcaca gctcgtccca ggcgtcgatc accgcgcgcg acgcgtcggg caccaggacc
     2281 agacgcaact cgccgaccgc cagcacgcgc ggcgcgatct gcggccgcaa cagcttggcg
     2341 aaccagtccg gcgcctgccg cccggccttg taggtcggcg gcggcgagcg gtaatgcagg
     2401 cggccgccgg catcgagcag ttgcagttcg ttggcgcgga tgcggccgag cggcgccagc
     2461 gccgcctgca ggtcggcggc gttcgccggc aggtgctcga cgagggccgc cagccactgc
     2521 ccggaaaccc ggttggcagc ctcgatttcc tcgtggatgg cctgccgcgc gccgtgcagc
     2581 cagaggccga cgaggaccgc gcagaggctg ccggccaagc cggtgaggac caagctgatg
     2641 cgggtgtgca ggcgtagcgg acgcatcgtg ctcacagtcc ggcgaagcgg tagcgcacgc
     2701 cgagccagcc ggcgcgcggc gcacccggcg cgacgaaggt ttcgcgccgc cagtcggcgc
     2761 tatcgacctg gaaggcgccg ttggcggaaa acgggttttc ggccagcgcg gcggccgtcg
     2821 catagcgttt gtcgaagacg ttgtcgaccc gcgcgaagac ctcccagccg ccgcccaggc
     2881 gggcctcggc gttgaggttg aggatggcgt agccgggcag gcggccgctg ccgctgaaga
     2941 cgcgggtgtt gccgaaggcg tcggtagcgc tgccggcgcg gtgcgcgttg ttctcgttgc
     3001 cgcgcgcgta ctgcccggag aaggcctgca ggtcgccgcc aaggcgcagc cagtcggcgg
     3061 cctgccagct caggccgagc ttgaggctgt gcgccggcag gccgggcagg cggtcgccct
     3121 tccgcacttc gatctcgtcg tcctggccgt tcgccgtgca ggcggcagaa gtgccgcgcg
     3181 agctgttgtt ctcggcgagc aggcaggcgg ccgagccgaa ggtggcgtgc agccagctgt
     3241 agctggcgaa ccagtcgaac ttgccgtgcc ggccgtcgat gccgagttcg acgccctggc
     3301 gccgggtgcg gccgaagttg gtgaagtaac cggcgctggt cgaggtgccg acgaacagga
     3361 tgtcgtcgtc ggcgacggtg cggaagacgc cggcattcca gccgatgccg ccagccagct
     3421 tgccgcgcag cccggcttcc agggtgcggg c
//
LOCUS       DAOSDX010000219         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9657_length_3448_cov_3.751842, whole genome shotgun sequence.
ACCESSION   DAOSDX010000219 DAOSDX010000000
VERSION     DAOSDX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_3448_cov_3.751842"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLN02_05990"
     CDS             <1..545
                     /locus_tag="PLN02_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HOI51909.1"
                     /translation="EFGGWQAGIDLDRFDRMYFPTRGWAAKVDYFNSPKLDYARLDVD
                     LRAAFSVADNVFNARLAYTGSPRGELPAFDAARLGGFLNMTAFSRDQLQGDSARYVGL
                     RTEHIISRMPLGLRGDLRLGLAVEAARLGGQLSGTRRRHWIDSTALYLGGETPLGPAY
                     LGLGRSSTGAANLFLFIGTP"
     gene            542..3202
                     /locus_tag="PLN02_05995"
     CDS             542..3202
                     /locus_tag="PLN02_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOI51910.1"
                     /translation="MSQTLYLCATSRLAQTLRQAVPAAGAVWRTPQALTLGQWLAQLA
                     DAALLAGEAVPIALDADAERLLWEQVVAEALAGGVAGAALFDLAGLAASAQEAHALCR
                     EWELTAGGDGDESRLFADWQARFVKRCRNAGWIDGGGLQHWLIERLNEGACALPERVV
                     VAGFDRPTPLERQLFAALAGRGVVVEMQPLAAVASQIEVRSGADAAAECHAVAAWVGE
                     RLAADPQARLGIVAPDLAGVRDRLAGLLDEVLLPAALRPDAADLARPYNISLGRPLAE
                     QPVVRSALELLAVAGSRGSVEQTRLSALLLGGFWAGAEGEADARARLDAAQRRRLGFL
                     TSPAALLRLGERLELPCPRTLAALAEGNGLAAAAPRSQRPAAWGRHFRQMLAALGWPG
                     ERSLSSHEFQARRAFLELLDGFGRLDGVLGSIGASTALARLRDLCRHRVFQPETRGRP
                     AVQVLGVLESAGLEFDALWVMGMNDDRWPPPPRPNPLLPAAVQRAAGSAHASAEVELD
                     FARRVQARLLRSAPQLIFSFAQADGNRLLRPSPLLAELPLAQPLTARVATLASRLALA
                     DQRVALADAKAPPVAPGEAVPGGSWLLRAQAICPAWAFYRFRLAAQALEAPVDGLDPA
                     ARGTLVHAALEAFWRACGGSAALAAQDVAARTAAIAAAVATALARFEQTLGGSLPARF
                     RQLEGGRLCRLLERWLTLEASRPQPFTVAACEQAQEVAIEGIRVKVVADRIDRLEGGR
                     QLIIDYKTGASIDVRNWASERLSEPQLPIYAALVADDVAGVAFAKVLADKPAFTGVAD
                     GGDLLPGVAGLADGRQKLFDPARFPDWQAVLEHWRACLQAVAREVGAGVAGVVFADEA
                     ELRYCEVLPLLRLPERRRLLAAAPPEGESG"
     gene            3236..>3448
                     /locus_tag="PLN02_06000"
     CDS             3236..>3448
                     /locus_tag="PLN02_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51911.1"
                     /translation="MTVKGWRLLGALVLALLAACGKEPAPAAPPRSTVTFLHYFTDSL
                     SGGVGETAQAFNAQNASHELKAVSLDH"
BASE COUNT          412 a         1285 c         1251 g          500 t
ORIGIN      
        1 ccgaattcgg cggctggcag gccgggatcg atctcgaccg cttcgaccgg atgtacttcc
       61 cgacccgggg ctgggccgcg aaagtcgact acttcaactc gccgaagctt gactacgccc
      121 ggctcgatgt cgatctgcgg gcggcgttct cggttgccga caacgtgttc aatgcccgtc
      181 tcgcctacac cggttcgccg cgcggcgaac tgcccgcgtt cgacgcggcg cgcctgggcg
      241 gcttcctcaa catgaccgcg ttcagccgcg accagctaca gggcgacagc gcgcgctacg
      301 tcggcttgcg caccgaacac atcatcagtc gcatgccgct cggcctgcgc ggcgacctgc
      361 gcctcggcct ggccgtcgaa gcggcgcgcc tgggcggcca actgagcgga acgcggcgcc
      421 gccactggat cgattcgacc gccctctacc tgggcggcga gacgccgctc ggtcccgcct
      481 atctcggcct gggccgctct tccaccggcg cggcgaacct cttcctgttc atcggcacgc
      541 catgagtcag acgctatacc tgtgtgccac ttcgcgcctc gcccagacgc tgcgccaggc
      601 ggtgccggcg gcgggcgccg tgtggcggac gccgcaggcg ctgacgctcg gccagtggct
      661 ggcgcaattg gccgacgccg cgctgctcgc cggcgaggcg gtgccgatcg cgctcgacgc
      721 cgacgccgag cgcctgctct gggaacaagt ggtcgccgaa gcgctcgccg gcggggtcgc
      781 cggcgccgcc ctgttcgatc tcgccggcct cgccgccagc gcccaggaag cgcacgcgct
      841 gtgccgggaa tgggagctga cggcgggcgg cgatggcgac gagtcacgcc tgttcgccga
      901 ctggcaggcg cgtttcgtga agcgctgccg caacgccggc tggatcgacg gcggcggcct
      961 ccagcactgg ctgatcgagc gcctgaatga aggcgcctgc gccttgcccg agcgcgtcgt
     1021 ggtcgccggt ttcgaccggc cgacgccgct tgagcgccag ttgttcgcag cgctggccgg
     1081 gcgcggcgtg gtcgtcgaga tgcagccgct ggcggccgtg gccagccaga tcgaggtacg
     1141 cagcggtgcc gatgccgccg ccgaatgcca cgccgtcgcc gcctgggtcg gcgaacgtct
     1201 ggcggccgat ccgcaggccc gcctcggcat cgttgccccc gacctggccg gcgtccgcga
     1261 ccgcctcgcc ggcctgctcg acgaagtcct gctgccggcg gcgctacgtc ccgacgccgc
     1321 cgacctggca cggccctaca acatctcgct cggccggccg ctcgccgagc agccggtggt
     1381 gcgcagcgcg ctcgaactgc tggcggtcgc cggcagccgc ggcagcgtcg aacagacccg
     1441 gctgtcggcg ctgctgctcg gcggcttctg ggccggcgcc gagggcgagg ccgatgcgcg
     1501 tgcccggctc gacgccgcgc agcgccgccg cctcggcttc ctgacctcgc cagcggctct
     1561 gttgcgcctc ggcgaacggc tggagctgcc ctgtccgcgc acactggccg cgctcgccga
     1621 gggcaacgga ctagccgcgg cggcgccgcg cagccagcgc ccggcggcct ggggccggca
     1681 cttccggcag atgctggcgg cgctcggctg gcccggcgaa cgctcgctgt ccagccacga
     1741 attccaggcc cggcgcgcct tcctcgaact gctcgacggt ttcggccggc tcgacggcgt
     1801 gctcggcagc atcggcgcgt ctaccgccct ggctcgcctg cgcgatctct gccggcaccg
     1861 cgtgttccag ccggaaaccc gcggccggcc ggcggtgcag gtgctcggcg tgctggagag
     1921 cgccggcctc gagttcgacg cgctgtgggt gatggggatg aacgacgacc gctggccgcc
     1981 gccgccgcgt cccaacccgc tgctgccggc cgccgtgcag cgagcggcgg gcagcgccca
     2041 cgccagcgcc gaggtcgagc tcgacttcgc ccgccgcgtc caggcccgcc tgctgcgttc
     2101 ggcgccgcaa ctgatctttt ccttcgccca ggccgacggc aaccgcctgc tgcggccgag
     2161 cccgttgctc gccgaactgc cgctggcgca gcccttgacg gcgcgggtgg cgacgctggc
     2221 cagccggctg gcgctggccg atcagcgcgt cgcgctggcc gacgccaagg cgccgccggt
     2281 ggcgcccggc gaagcggtgc ccggcggtag ctggctgttg cgcgcccagg cgatctgccc
     2341 ggcctgggcc ttctaccgtt tccggctcgc cgcccaggcg ctcgaagcgc cggtcgacgg
     2401 gctcgacccg gcggcccgtg gcacgctggt gcacgccgcg ctcgaagcct tctggcgcgc
     2461 ttgcggcggc agtgcggcgc tcgccgcgca ggacgtcgcc gcccggacgg cggccatcgc
     2521 cgccgctgtc gctacggcgc tggcgcgctt cgagcagacg ctgggcggca gtctgccggc
     2581 gcgtttccgg cagctcgaag gcggccgcct gtgccgcctg ctcgagcgct ggctgacgct
     2641 cgaggcgagc cggccgcagc cattcaccgt cgccgcctgc gagcaggcgc aggaagtcgc
     2701 gatcgaaggc atccgggtca aggtggtggc cgaccgcatc gaccgcctgg aaggtggccg
     2761 ccaactgatc atcgactaca agaccggtgc cagcatcgac gtccgcaact gggcgagcga
     2821 gcgcttgagc gagccgcaac tgccgatcta cgcggcgctg gtggcggacg atgtggccgg
     2881 cgtcgccttc gccaaggtgc tcgccgacaa gcccgcgttc accggcgtcg ccgacggcgg
     2941 cgatctgctg cccggcgttg ccgggctggc cgacggccgc cagaagctgt tcgacccggc
     3001 gcgcttcccg gactggcagg cggtgctcga gcactggcgg gcgtgcctgc aggcggtcgc
     3061 ccgcgaggtc ggcgccggcg tcgctggcgt cgtcttcgcc gacgaggcgg aactgcgtta
     3121 ttgcgaggtg ctgccgctgc tccgtctacc cgagcggcga cgcctgctgg cggctgcgcc
     3181 gccggagggt gagtcgggat aagatggaga ctggcatgca acggggagag aggcgatgac
     3241 ggtcaagggc tggcggttgc tcggcgctct cgtcctcgct ttgcttgcgg cctgcggcaa
     3301 ggaaccagcg ccggctgcgc cgccgcgcag cacggtgacc ttcctgcatt acttcacgga
     3361 ttccctgagc ggcggcgtcg gcgagaccgc ccaggcgttc aatgcgcaga acgccagcca
     3421 tgagctgaag gcggtttccc tcgatcac
//
LOCUS       DAOSDX010000220         3440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9699_length_3440_cov_2.639882, whole genome shotgun sequence.
ACCESSION   DAOSDX010000220 DAOSDX010000000
VERSION     DAOSDX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_3440_cov_2.639882"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1763
                     /locus_tag="PLN02_06005"
     CDS             156..1763
                     /locus_tag="PLN02_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51912.1"
                     /translation="MSKQEVALKPIGRPRKIDLAEEMELAGVVVPQEVADAIPAEEPV
                     FLGYQQRWFEDESQIMIGEKSRRTGLTWAEAGRNVVKAAKPRRRQGCNTFYVGSKKEM
                     ALEYIAACALFAKAFNELAQADVYEQTFWDEGKQEEILTYMIRFPKSGFKIQALSSRP
                     SNLRGLQGDVVIDEAAFHESLEELLKAALALTMWGNKVRLISTHNGVENLFNEYIEEA
                     RAGKKDYSVHRITLDDAIADGLYKRICYVTGQTWSPEAEKKWRNDLYRNAPNQESAEE
                     EYGCIPKNGGGAWLSRALIESRMSADTPVLRWECKAGFELLPDHIRAAECRDWLEASL
                     APLLAALPADAISFNGEDFGRTGDLSVHVPLIQTQNLVRRVPFLVELRNVPFRQQEQI
                     AFYLLDRLPRFTGGAFDARGNGQFLAEICMQRYGASRIQQVMLSEGWYREHMPPVKAA
                     LEDGNLDCLPRDADVLADLRAVQVIKGVPRIPETRTTGEDKGKRHGDAAVAVALAYFA
                     SREINKGPVTVKSRRHRTGARITQGFV"
     gene            1760..>3080
                     /locus_tag="PLN02_06010"
     CDS             1760..>3080
                     /locus_tag="PLN02_06010"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HOI51913.1"
                     /translation="MSRAKGMWVTPTEFVQFGEPRKSLSDQIATRSRSIDFYGLGTYL
                     PNPDPVLKALGKDIKVYRELRADAHVGGCIRRRKAAVKALEWGLDRDKAKSRVAKSVE
                     AIFADLDLSRIITEMLDAVLYGYQPMEVMWGKVGGYLVPVDIVGKPADWFVYDEGNKL
                     RLRTKQSPLKGEELPPRKFLVPRQDASYDNPYGFADLSMCFWPTTFQKGGLKFWVQFT
                     EKYGAPWVIGKHPRNTPDTETDKLLDSLEAMVQDAVAVIPDDSSVEIQEAAGKTGSTE
                     VYERLLHFCRSEVSIALLGQNQTTEATANRASAQAGLEVTRDIRDGDKAVAVEAFNTL
                     IRWVCELNFNDGARPVFEMWEQQEVDKVLAERDEKLVRAGAKLTPAYFKRAYSLQDGD
                     LDEAAKPATPAAEFAEGEEAPDQDALDAAMDALSADAMNADAQAMLAP"
     assembly_gap    3081..3090
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3258..>3440
                     /locus_tag="PLN02_06015"
     CDS             3258..>3440
                     /locus_tag="PLN02_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51914.1"
                     /translation="MTLPPRKAVEYLKSKGYAITWHWEELWQEAQAQAFTVAKATRLD
                     ILQDIREAVEKALAEGK"
BASE COUNT          701 a         1097 c         1113 g          519 t
ORIGIN      
        1 aagttccgcc tggaagagca ggcacgcatc gagcgcgaag cacgcacgag gctgcttgcc
       61 gaacaggaag aaaagctcca ggagctgcgc ggcgctgacg gcatgagcga gcagatggaa
      121 gctcgcatcc gccgcatcct gctcgggaaa gagtgatgtc aaagcaggaa gtcgccctca
      181 aaccgatcgg ccgtccccgc aagatcgacc tggccgagga gatggagctg gccggcgtcg
      241 tcgtgccgca ggaggtggct gatgccatcc cggctgaaga gcccgtcttc ctgggctatc
      301 agcagcggtg gtttgaggat gaatcccaga tcatgatcgg ggaaaaatcc cgccgtaccg
      361 gtctgacctg ggcggaagcc ggtcgcaacg tcgtgaaggc tgcaaagccg cgccgtcgcc
      421 agggctgcaa cactttttat gtcggcagca aaaaggagat ggcgctggag tacatcgccg
      481 cctgcgcgct gttcgccaag gcgttcaacg aactggccca ggctgacgtg tacgagcaaa
      541 ccttctggga cgaaggaaag caggaagaaa tcctgacgta catgatccgg ttccccaagt
      601 ccggcttcaa gattcaggcg ctgtcgagtc ggccgtccaa tctacgcggc ctgcagggcg
      661 atgtcgtgat cgacgaagcg gccttccatg aaagcctgga agagctgctg aaggccgcgc
      721 tggcgctaac catgtggggc aacaaggtcc ggctgatcag cacgcacaac ggcgtcgaga
      781 acctcttcaa cgagtacatc gaggaggccc gtgccggcaa gaaggactac agcgttcacc
      841 gcatcacact ggacgacgcc attgccgacg gcctctacaa gcgcatctgc tacgtcaccg
      901 gacagacctg gtcgccggaa gcggaaaaga agtggcgtaa cgacctgtac aggaacgccc
      961 cgaaccagga gagcgccgaa gaggagtacg gttgcatccc aaagaatggc ggcggcgcct
     1021 ggctgtcccg cgccctgatc gagtcgcgca tgtcggccga cacgccggtg ctgcgctggg
     1081 agtgcaaggc cggcttcgag ctgctgcccg accacatccg cgccgccgag tgccgcgact
     1141 ggctggaagc cagcctggcg ccgctactgg ctgccctgcc agcagacgcc atttcattca
     1201 acggcgagga cttcggccgc accggcgacc tgagcgtgca tgtgccgctg atccagacgc
     1261 agaacctggt gcgccgcgtg cccttccttg tcgagctgcg caatgtgccc ttccgccagc
     1321 aggaacagat cgccttctac ctgttagacc gcctgccgcg cttcaccggt ggcgcatttg
     1381 atgcacgggg caacggccag ttcctggccg agatttgcat gcagcgctac ggggcgtcac
     1441 gcatccagca ggtcatgctg tctgagggct ggtaccgcga acacatgccg ccggtcaagg
     1501 ccgccctcga ggacggcaac ctggactgcc tgccgcgtga cgccgacgtg ctggccgacc
     1561 tgcgggcggt gcaggtcatc aagggcgtgc cgcgcatccc cgagacccgg acgaccggcg
     1621 aggacaaggg taagcgccac ggcgatgccg ccgtggcagt ggcgctggcc tacttcgcca
     1681 gccgcgagat caacaaaggc ccggtaacgg tgaaatcccg ccgccatcgc accggcgcca
     1741 gaattaccca aggattcgta tgagcagagc caaaggcatg tgggtcaccc ccaccgaatt
     1801 cgtccagttc ggagaaccgc gcaagtcgct ctccgatcag atcgccacgc gcagccgtag
     1861 catcgacttc tacggattgg gcacctatct gcccaatccc gacccggtgc tgaaagcact
     1921 gggcaaggac atcaaggtct atcgcgagct gcgcgccgac gcccacgtcg gcggctgcat
     1981 ccggcgccgc aaggcggccg tcaaggctct cgaatggggg ctggaccgcg acaaggcaaa
     2041 gagtcgcgtg gccaagtccg tcgaggcgat cttcgccgac ctggacctgt cgcggatcat
     2101 taccgagatg ctggatgcgg tgctctacgg ctaccagcca atggaggtga tgtggggaaa
     2161 agtcggcggc tatcttgtgc cggtcgacat cgtcggcaag ccggccgatt ggttcgtgta
     2221 cgacgagggc aacaagttgc gcctgcgcac caagcaaagc ccactcaagg gcgaggaact
     2281 gccgccgcgc aagttcctgg tgccgcgcca ggacgcaagc tacgacaacc cctacggctt
     2341 cgccgacctg tccatgtgct tttggccgac caccttccag aaaggcggac tcaagttctg
     2401 ggtccagttc accgagaaat acggggcccc ttgggtcatc ggcaagcatc ctcggaatac
     2461 gccggatacc gagaccgaca agctgctcga tagcctggag gccatggtcc aggatgcggt
     2521 ggcggtgatc ccggacgatt ccagcgtcga gattcaggag gccgccggca agaccggcag
     2581 caccgaggtc tatgagcgcc tgcttcactt ctgccgctcc gaggtgtcga tcgcgctgct
     2641 cggccagaac cagaccaccg aggctacggc aaaccgcgcc tctgcccagg ccgggctgga
     2701 ggtcacccgg gacatccgcg atggcgacaa ggccgtcgcc gtcgaagcct tcaacaccct
     2761 gatccgctgg gtgtgcgagc tgaatttcaa cgacggcgcc cggccggtgt tcgagatgtg
     2821 ggagcagcag gaggttgaca aggtactggc cgagcgcgac gagaagctgg tgcgggccgg
     2881 agccaagctg acccccgcct acttcaaacg cgcctacagc ctgcaggacg gcgacttgga
     2941 cgaggcggcc aaacccgcca ccccggccgc cgagtttgcc gagggcgagg aagcacccga
     3001 ccaggacgcg ctggatgccg ccatggacgc actgtcggcc gacgccatga atgcggacgc
     3061 ccaggccatg ctggcgccgc nnnnnnnnnn cctaaagcgg gtcgccaagg gtgcccaacc
     3121 tgacgagctg ctcggcatgc tcgccgagct gtatccggaa atggacgcca ccggcctgca
     3181 ggatcgcctg gcacgcatga ccttcgtggc caacctgtgg ggacggctgc atgcctaaag
     3241 tggatctagc ctactgcatg accctgccgc ccaggaaggc ggtcgagtac ctgaagagca
     3301 agggctacgc gatcacctgg cactgggaag agctttggca ggaagcgcag gcccaggcgt
     3361 tcaccgtggc caaggccacg cgcctggaca tcctgcagga catccgcgag gcggtggaaa
     3421 aggcgctcgc cgaaggcaag
//
LOCUS       DAOSDX010000221         3436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9712_length_3436_cov_3.343094, whole genome shotgun sequence.
ACCESSION   DAOSDX010000221 DAOSDX010000000
VERSION     DAOSDX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9712_length_3436_cov_3.343094"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLN02_06020"
     CDS             <1..517
                     /locus_tag="PLN02_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HOI51915.1"
                     /translation="TSVHVIFLVGVVLFAHHAVVFMGLFLFFLGYTEAYKRHQERLIL
                     REGLMVAFFLAGLVVLGGLQQWWLQAALTGVEPTVLYFLATALTAVTDNAALTYLASL
                     VDGVSMEFKYAIVAGAVTGGGLTVIANAPNPAGVAILKGYFDDESISFVGLFLAAFPP
                     TCMAIFAFQAL"
     gene            672..1658
                     /locus_tag="PLN02_06025"
     CDS             672..1658
                     /locus_tag="PLN02_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOI51916.1"
                     /translation="MSATSSPATALGANHGYRFAGDSVHLNAEVTLAASDLQDGSTWS
                     LQLWASGAGFAERAPAGVKVGEFPLQPLPGCFNVDACVAALPPAGHDAFTMALMLVSG
                     DTDGGVEVRDLAIYPTPQYFVQPTLGGALDCRIADGLAEIVIESIANPRAADNLSGTL
                     ALEVWALDSPYAGGAWTGTPVASLVLGQLAGGEAWSDCRYTVPAVDPAGAALTVMLRE
                     WTASGYLTRDYRNLPLVAAVASAAVAPAPTASPAKPAAKKAAKAPAVAAQAATVSVNA
                     ASVEELAALKGLSRAVAAAIVAGRPYASVAELLRVKGLGPKLLEKLGASLRV"
     gene            1779..3176
                     /gene="nifE"
                     /locus_tag="PLN02_06030"
     CDS             1779..3176
                     /gene="nifE"
                     /locus_tag="PLN02_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HOI51917.1"
                     /translation="MKTSEIQALLDEPACSHNHKEKSGCAKPKPGATAGGCAFDGAQI
                     ALLPIADVAHIVHGPIACAGSSWDNRGARSSGVTLYKIGMTTDLSETDVVMGRSEKRL
                     FHAIKQAIDSYAPAAVFIYNTCVTALIGDDVEAVCKAATERWGVPVVPVDAAGFYGTK
                     NLGNRLAGEAMFKHVIGTREPEPAKPRPDGLPTYDINLIGEYNIAGEFWHVAPLFDEL
                     GYRILCTLSGDSRFHEVQTMHRAKVNMVVCAKALLNVARKMQENYGQPYFEGSFYGVQ
                     DVSNALRDFARLIGDPDLIARTETVIAREEAKAHAALEPWRARLKDKRVLLYTGGVKS
                     WSIVSALQDLGMKVVATGTKKSTEEDKARIRELMGEDTKMIDDGSPKALLSTYHEYKA
                     DILIAGGRNLYTALKARIPFLDINQEREFGYAGYAGMSELARQLALSMESPVWAAVRK
                     PAPWAKGVSGTALAA"
     gene            complement(3184..>3436)
                     /locus_tag="PLN02_06035"
     CDS             complement(3184..>3436)
                     /locus_tag="PLN02_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51918.1"
                     /translation="GLRKPAELWIHERMTYYALMDLQREAAFVLDEASPPELVEKYFF
                     RLQEILGASNDKWNRQIVGKRETQAADAAGADRPAPANG"
BASE COUNT          572 a         1166 c         1094 g          604 t
ORIGIN      
        1 gacttcggtg cacgtcatct tcctcgtcgg cgtcgtcctc tttgcccacc atgccgtggt
       61 cttcatgggc ctgttccttt ttttcctcgg ctacaccgaa gcctacaagc ggcaccagga
      121 gcggctgatc ctgcgcgaag gcctgatggt cgccttcttc ctcgccggcc tggtggtcct
      181 cggtggcttg cagcaatggt ggctgcaggc ggcgctgacc ggggtcgagc cgaccgtgct
      241 ctacttcctg gccaccgcgc tgaccgcggt gaccgacaac gccgccttga cctacctggc
      301 ctcgctggtt gacggcgtgt cgatggagtt caagtacgcg atcgtcgccg gcgcggtgac
      361 cggcggtggc ctgacggtca tcgccaacgc gccgaacccg gccggcgtcg ccatcctgaa
      421 gggctatttc gacgacgagt cgatcagttt cgtcggtctc ttcctggccg ccttcccgcc
      481 cacctgcatg gcgatcttcg ccttccaggc gctgtgacgc cggatggccg ggaaaccggc
      541 catctgtcgt ttttgcgaca agcgaggttt tttggatgca ccttcattgt gcataaaaaa
      601 tcgccttaag cgattgattg atttaaatat tttgcggtct ataatgcgcg gcctttttcc
      661 cggagaagac cgtgtccgca acctcgtccc cggctaccgc cctcggtgcc aaccatggct
      721 atcgcttcgc tggcgatagc gtccatctca acgccgaagt cacgctcgcc gccagcgatc
      781 tgcaggatgg cagcacctgg tcgctgcaac tgtgggcgag cggcgccgga tttgccgagc
      841 gcgcaccggc cggcgtcaag gtcggcgaat ttccgctgca gccgttgccc ggctgtttca
      901 atgtcgatgc ctgcgttgct gccttgccgc cggccggaca cgacgccttc acgatggcgc
      961 tgatgctggt ttccggtgac accgatggtg gcgtcgaggt ccgcgacctg gcgatctatc
     1021 cgacgcccca gtacttcgtc cagccgacgc tgggcggcgc gctcgactgc cggatcgccg
     1081 atgggctcgc cgagatcgtc atcgaaagca tcgccaaccc gcgcgccgcc gacaatctca
     1141 gcggcacgct ggcgctcgaa gtgtgggcgc tggacagccc ctacgccggt ggcgcctgga
     1201 ctggcacgcc ggtggccagc ctggtcctcg gtcaattggc cggcggcgaa gcctggtcgg
     1261 attgccgcta cacggtgccg gcggtcgacc cggccggcgc ggcgctgacc gtgatgctgc
     1321 gcgaatggac ggcaagcggc tacctgacgc gcgattaccg caacctgccg ctggtcgcag
     1381 cggtggcatc cgccgctgta gcaccagcgc cgaccgcctc gcctgccaag ccggcggcga
     1441 agaaggcagc gaaagcgccg gctgtcgcgg cgcaggccgc cacggtctcg gtcaacgcgg
     1501 cgagcgtcga agaactggca gcgctcaagg ggctcagccg cgccgtcgcg gcggccatcg
     1561 tcgccggccg tccttacgcc tcggtcgccg agctgctgcg ggtcaagggg ctcggcccca
     1621 agctgctgga aaagctcggc gccagcctgc gcgtctgagc aaggacaagg cttgtcgcaa
     1681 aggcgacaag ccttgtctgg ctactaattc ttgaaaatca gtggcttggc gcgtggcacc
     1741 catcttgctg agtagtctgc ggactaccgg agatgcgcat gaaaaccagc gaaatccagg
     1801 ccctgctcga cgagcccgcc tgcagccaca accacaagga aaagtcgggc tgtgccaagc
     1861 ccaagcctgg cgccacggct ggcggttgcg ccttcgacgg cgcgcagatc gcgctcttgc
     1921 cgatcgccga cgtcgcccac atcgtgcacg ggccgatcgc ctgcgccggg tcttcgtggg
     1981 ataaccgcgg cgcgcgctcc tcgggcgtta ccttgtacaa gatcggcatg accaccgacc
     2041 tgtcggaaac cgacgtcgtc atggggcgca gcgagaaacg cctgttccat gcgatcaagc
     2101 aggcgatcga cagctacgcg ccggccgccg tcttcatcta caacacctgc gtcaccgcgc
     2161 tcatcggcga cgatgtcgag gcggtctgca aggccgccac cgagcgctgg ggcgtgcccg
     2221 tggtgccggt cgacgcggcc ggcttctacg gtaccaagaa cctcggcaac cgcctggccg
     2281 gcgaagcgat gttcaagcac gtcatcggca cgcgcgaacc ggaaccggcg aagccgcgcc
     2341 cggacggcct gccgacctac gacatcaacc tgatcggcga gtacaacatc gccggcgagt
     2401 tctggcacgt cgcaccgctg ttcgacgaac tcggctaccg catcctgtgc acgctgtccg
     2461 gcgattcccg cttccacgag gtgcagacca tgcaccgggc gaaggtgaac atggtggtct
     2521 gcgccaaggc gctgctcaac gtcgcccgca agatgcagga aaactacggc cagccctact
     2581 tcgagggcag cttctacggc gtccaggacg tttccaatgc cttgcgcgac ttcgcccggc
     2641 tgatcggcga ccccgacctg atcgcccgta ccgagacggt gatcgcccgc gaggaagcca
     2701 aggcgcacgc cgccctcgaa ccctggcgcg cccgcctcaa ggacaagcgc gtcctgctct
     2761 acaccggcgg cgtcaagtcg tggtcgatcg tctccgcgct gcaggatctc ggcatgaaag
     2821 tcgttgccac cggtaccaag aaatcgaccg aagaggacaa ggcgcgcatc cgcgaactga
     2881 tgggcgagga caccaagatg atcgacgacg gcagcccgaa ggcgctgttg tcgacctatc
     2941 acgagtacaa ggccgacatc ctgatcgccg gggggcgcaa cctgtacacc gcactgaagg
     3001 cgcgcattcc cttcctcgac atcaaccagg agcgcgaatt cggctacgcc ggctacgctg
     3061 gcatgagcga actcgcccgc cagctcgccc tgtcgatgga aagcccggtg tgggccgcgg
     3121 tcaggaaacc ggcgccgtgg gcgaaaggcg tttcgggaac ggcgctggcg gcctgaaccg
     3181 gcttcagccg tttgccggcg ccggtcggtc cgcgccggcg gcgtccgctg cctgtgtctc
     3241 gcgcttgccg acgatctgcc ggttccactt gtcgttggag gcgccgagga tttcctgcag
     3301 gcggaagaag tatttttcga ccagttccgg cggacttgcc tcgtcgagga cgaaagccgc
     3361 ctcgcgttgc agatccatca gcgcgtagta ggtcatgcgc tcgtgtatcc acagttcggc
     3421 aggcttgcgc aggcct
//
LOCUS       DAOSDX010000222         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9764_length_3420_cov_3.106389, whole genome shotgun sequence.
ACCESSION   DAOSDX010000222 DAOSDX010000000
VERSION     DAOSDX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9764_length_3420_cov_3.106389"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..811)
                     /gene="rpiA"
                     /locus_tag="PLN02_06040"
     CDS             complement(143..811)
                     /gene="rpiA"
                     /locus_tag="PLN02_06040"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOI51919.1"
                     /translation="MTQDELKQAVAQAAADHVAESTPVGAIIGVGTGSTANFFIDALA
                     ALKDRIGGAVASSEATRQRLAGHGIPVLDLNEVSRIPVYVDGADEIDDGLNMIKGGGG
                     ALTREKIVAAVADCFVCIADASKLVDTLGKFPLPVEVIPMARAHVIRELEKLGGLPQL
                     REGFVTDNGNQIVDVRGLLIHDPKGLEAKINQIVGVVTNGLFAMRPADLLLLGTSEGV
                     RRIG"
     gene            920..1477
                     /locus_tag="PLN02_06045"
     CDS             920..1477
                     /locus_tag="PLN02_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51920.1"
                     /translation="MTTKENPSPSPVVQPAEAVGTAAAPASAPAKAPAKAPAKAPAKA
                     PARARAPRKTPSVEQTVAKHQQALAEALEKAEAISYDPPQAPKAAKPAKATAKTKAEK
                     VEKVEKPAKLKKPRLVRDSYAMPETEYAQIAVLKKRMASLGSEAKKSELLRAGIAVLA
                     ALNDAELQAVMGRIERIKTGRPAKK"
     gene            complement(1500..2432)
                     /locus_tag="PLN02_06050"
     CDS             complement(1500..2432)
                     /locus_tag="PLN02_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI51921.1"
                     /translation="MKKILLALTGALMLFAQTLRAEAVFTVDALGNGVFALIGQTGGR
                     LYENHGLNANYGVIDTAAGTILIDAGASAVAARVLEMEARKLTGKPVKWVINTGAQDH
                     RWLGNAYFAAQGAQIIALQRTATTQTRLGKGQIEALGRDLKEQMEGTAPAPAAQPLAG
                     DTQTLTLGGRTLELRYFDHAHFAGDAVVWLPQEKILFSGDHIYVDRLLGILPESNAET
                     WLKAFRQAIALQPQRIVPGHGRVCDVAQAQRETGDYLAFLVDGSRKLAEEMAGVDAAV
                     AALQDAPAFAGLKNFADLHRGNVNRAYLRFEAGQ"
     gene            complement(2466..2696)
                     /locus_tag="PLN02_06055"
     CDS             complement(2466..2696)
                     /locus_tag="PLN02_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOI51922.1"
                     /translation="MKDIKVLGTGCANCRNTVKLIEEVAAEKGVAITLSKVEDLPEIM
                     KYGVMSTPGVVVDGKVVHAGGIPDRAKISSWL"
     gene            complement(2708..>3420)
                     /locus_tag="PLN02_06060"
     CDS             complement(2708..>3420)
                     /locus_tag="PLN02_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOI51923.1"
                     /translation="PFCSCSAVPLFIGFLSAGVPLGVTFSFLISAPMVNEVALALLFG
                     LFGWQVAALYLGLGLLVAIVAGLVIGQLKMERHLEDWVQQIQRGEATWNDDGSNGWPA
                     RFAAGWRHVREIVAKVWPYILVGIALGAGIHGYVPEDFMASFMGKDAWWAVPAAVVLG
                     VPMYTNAAGIIPIVEALIGKGAALGTVLAFMMSVIALSAPEMIILRKALKPTLIATFA
                     GVVATGILLVGYVFNLVL"
BASE COUNT          630 a         1079 c         1139 g          572 t
ORIGIN      
        1 ccaggtagtg cgaggagagg gcaagcaggt tgccgagcgc ctgttcgcgg ctggcggcga
       61 tttcctgggc ggacaagaac atggggcgct cccggctgtc gaatgccggc agtctggcgc
      121 cccgatgtga caggccgatg actcagccga tgcggcgcac gccctcgctg gtgccgagca
      181 gcagcagatc ggccgggcgc atcgcgaaca gcccgttggt gacgacgccg acaatctggt
      241 tgatctttgc ttccaggcct ttcggatcgt ggatcaggag gccgcggacg tcgacgatct
      301 ggttgccgtt gtcggtgacg aagccctcgc gcagttgcgg caggccgccg agtttctcga
      361 gttcgcggat gacatgggcg cgggccatcg ggatgacttc caccggcagc gggaatttgc
      421 ccagcgtgtc gaccagcttc gaggcgtcgg cgatgcagac gaagcagtcg gcgacggcgg
      481 caacgatctt ttcgcgggtg agcgcgccgc cgccgccctt gatcatgttg aggccatcgt
      541 cgatttcgtc ggcgccgtcg acatagacgg gaatccggct gacctcgttg aggtcgagca
      601 ccgggatgcc gtggccggca aggcgctggc gggttgcctc ggaactggcg acggcaccgc
      661 cgatgcggtc cttcagcgca gccagcgcat cgatgaagaa attggccgtc gagccggtgc
      721 caacgccgat gatggcgccg accggcgtgc tttccgcaac atgatcggca gcggcctggg
      781 cgaccgcctg cttgagttcg tcctgggtca tggggggctc ctgggatggt ttgccgcgat
      841 tctactgtca aaaaagttta accacgtgac agtttgaaat cctctaaact ggatataccg
      901 tttttaggga gaacccgcag tgactaccaa agaaaatcct tccccgtccc ccgtcgtcca
      961 gcctgccgaa gccgtaggca ccgccgcagc acccgcgtcg gcaccggcca aggcaccggc
     1021 caaggcaccg gccaaggcac cggccaaggc accggccagg gcccgtgcgc cgcgcaagac
     1081 accgtcggtc gaacagaccg tcgccaaaca ccagcaggcg cttgccgaag cgctggaaaa
     1141 ggccgaagcc atcagctacg acccgcctca ggccccgaag gcggcaaagc cggccaaggc
     1201 caccgccaag accaaggcgg aaaaggtcga gaaagtcgaa aagccggcca agctgaagaa
     1261 gccgcggctg gtacgcgaca gctacgcgat gccggaaacc gaatacgcgc agatcgccgt
     1321 actcaagaag cggatggcaa gcttgggtag cgaagccaag aagagcgaac tgctgcgcgc
     1381 cggcatcgcc gttctggcag ccctcaacga cgccgaactg caggcggtga tggggcgcat
     1441 cgagcggatc aagaccgggc ggccggcgaa gaagtgaact ccggggccgg ccggcctgct
     1501 tactggccgg cctcgaagcg caggtaggcg cgattgacgt tgccccggtg cagatcggcg
     1561 aagttcttca ggccggcgaa ggccggggca tcctgcagcg cggcgacggc agcgtcgacg
     1621 ccggccattt cctcggccag cttccggcta ccgtcgacca ggaaagccag gtagtcgccg
     1681 gtttcgcgtt gcgcctgcgc cacgtcgcag acccggccgt gaccgggaac gatgcgttgc
     1741 ggttgcaggg caatggcttg tctgaaagcc ttgagccagg tttcggcgtt gctttcaggc
     1801 aggatgccga gcaggcggtc gacgtagatg tgatcgccgg agaacaggat cttctcctgc
     1861 ggcagccaga ccacggcatc gccggcaaag tgggcatggt cgaagtaacg cagctccagc
     1921 gtccggcctc ccagcgtcaa ggtctgggta tcgccggcca gcggctgggc ggcgggcgcg
     1981 ggggcggtgc cctccatctg ttccttcagg tcgcggccca gcgcttcgat ctgtcccttg
     2041 ccgagacggg tttgcgtcgt cgccgtgcgt tgcaaggcga tgatctgtgc gccctgggcg
     2101 gcgaagtaag cgttacccag ccagcgatgg tcctgggcac cggtattgat cacccatttg
     2161 accggcttgc cggtcagttt tctggcctcc atttcaagga ccctggcggc gacggccgag
     2221 gccccggcat cgatcaggat cgtccccgcc gctgtgtcga tcacgccgta attggcgttc
     2281 aggccgtggt tttcgtacag acggccgccg gtctggccga tcaaggcgaa aacgccgttg
     2341 cccagggcgt cgaccgtgaa tacggcctcg gcacgaagcg tctgggcgaa cagcatcaag
     2401 gcgccagtca gcgccagcaa tatcttcttc attgtctctt cctcctccgt tggggtttta
     2461 ttcgtttaca accagctact gatcttggcc cgatccggga tgccgccagc gtgtaccacc
     2521 ttgccatcga cgaccacgcc cggtgtcgac atcaccccgt atttcatgat ctccggcaaa
     2581 tcttcgacct tgctcagggt aatggcgacg cctttttcgg cggcgacttc ctcgatcagt
     2641 ttgaccgtgt tgcggcagtt ggcgcagccg gtgccgagca ccttgatgtc tttcatgctt
     2701 tttctcccta gaggacgagg ttgaacacgt agccgacgag caggatgccg gtggcgacga
     2761 cgccagcgaa ggtggcgatg agggtgggtt tgagcgcctt gcgcaggatg atcatctccg
     2821 gcgccgagag tgcgatgacg ctcatcatga aggcgagcac ggtgccgagc gcggcgccct
     2881 tgccgatcag tgcctcgacg atcgggatga tgccggcggc gttggtatac atcggcacgc
     2941 cgagcacgac ggcggccggc accgcccacc aggcatcctt gcccatgaac gaagccatga
     3001 agtcttccgg cacgtagccg tggatgccgg caccgagcgc gatgccgacg aggatgtagg
     3061 gccagacctt ggcgacgatc tcgcgcacgt gacgccagcc ggcggcgaag cgcgccggcc
     3121 agccgttcga cccgtcgtcg ttccaggtgg cttcgccgcg ctggatctgc tgcacccagt
     3181 cttctaggtg gcgttccatc ttcagctggc cgatgaccag cccggcgacg atggcgacca
     3241 gcaggccgag tcccaggtag agcgccgcga cctgccagcc gaagaggccg aagagcagcg
     3301 ccagcgccac ttcattgacc atcggcgccg agatcaggaa ggaaaaggtc acgccgagcg
     3361 gcacgccggc tgacaggaaa ccgatgaaca gcggcaccgc cgagcacgaa cagaagggag
//
LOCUS       DAOSDX010000223         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9784_length_3414_cov_3.471569, whole genome shotgun sequence.
ACCESSION   DAOSDX010000223 DAOSDX010000000
VERSION     DAOSDX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9784_length_3414_cov_3.471569"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLN02_06065"
     CDS             complement(<1..444)
                     /locus_tag="PLN02_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOI51924.1"
                     /translation="MPLPAFLRRRGVGPALAAAVVLGGVATWLAGDRVDYETTPVVRA
                     DIEATVTAIGTLQPRRYVDVGAQVSGQIVRLHVQPGSQVAQGDLLVEIDPSVQRATVD
                     AGRAALAGLRAQLAEQQAQRLLAQQQNERQQELALAGATRLEDVQT"
     gene            complement(444..1064)
                     /locus_tag="PLN02_06070"
     CDS             complement(444..1064)
                     /locus_tag="PLN02_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51925.1"
                     /translation="MQRFLSVLPGRRWLPVFALCAASTVHAEYFWIDADAGGRRIVAG
                     ELGKPQALAAARDARAFAADGKAVALNAADDGYQVAAAAGDLRFTARRGDDKTLVIYH
                     ARYGRQETKAVSDLELVPTTPGGNTYRLFWKGSPVAASQVNVATSAGWSRVLRPAADG
                     SVSFDPAFPALYVLEVTARINGSASVDGKKYDDVRHVATLSFRIEP"
     gene            complement(1084..1428)
                     /locus_tag="PLN02_06075"
     CDS             complement(1084..1428)
                     /locus_tag="PLN02_06075"
                     /inference="COORDINATES: protein motif:HMM:NF023232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HOI51926.1"
                     /translation="MDPHFALYLVFFVCYAGLLTLSLAMDRHHSQIRHGKPAPLLRRA
                     LRLAGWSLLALALALWLCSLVWGWAIGPVAWFGLLSTAALGFVFLLPYAPRFAAWLAV
                     IGPLLALPPFFL"
     gene            complement(1430..3055)
                     /locus_tag="PLN02_06080"
     CDS             complement(1430..3055)
                     /locus_tag="PLN02_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOI51927.1"
                     /translation="MKQTLRQSMSWLHTWGGLWFTWILFAIFLTGTLGVFDDPISHWM
                     REQGKIGEIAPSTVDQRSRAVELGQRHLAAVSPRADFWSIGLPGDENPNLRVFWRADE
                     DAPFKQALLDPLTGAELQPGASRETEGGHHFVHMHFEFHAGMAGIWIVGAATMAMLVA
                     LVSGIIVHKKIFADFFTFRPGKGQRSWLDGHNALGVLTLPFLFVISYTGLTIFWATYM
                     PAGVLARYDGSEEAFFARLSEDPAPRPLQHIDAPMPELAPLMRRGEAAIGREASFVVV
                     EHAGDRSAAVRVFGRFDPDAENDRLVGRGSGSALYDAITLETLDVQLPNTVRGGSVLQ
                     TQRTMNALHFAHFGGYPMKWLYFICGMAASAMLATGAILFMVKRRKKSANEFGAATAR
                     VYRGIEVLNVAAIAGLILACAAYFWINRLLPLDVAERGTREIVLFFAVWLASLAHAAL
                     RPSLAAWREQLAACALLCLGLPLLNLATTGDWLVAALARGDGETAGVELSAIGFGLIF
                     LAMLRQLRRRAPTLTVDRPETAKTAVIDAIGGR"
     gene            complement(3052..3351)
                     /locus_tag="PLN02_06085"
     CDS             complement(3052..3351)
                     /locus_tag="PLN02_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3649 domain-containing protein"
                     /protein_id="HOI51928.1"
                     /translation="MSVPSPAHPRLALGSRVLAAVVGGYALASVFSIWLSYLLPAELP
                     EAVLGATLFSFAVYTAAVIWVFAAASATRAWLGLLLPGAAMGLMAAALQLGGAAA"
BASE COUNT          546 a         1129 c         1308 g          431 t
ORIGIN      
        1 cgtctgcacg tcctccaggc gggtggcgcc ggcgagcgcc agttcctgct ggcgttcgtt
       61 ctgctgctgg gcgagcaggc gctgcgcctg ctgttcggcc agctgggcgc gcaggccggc
      121 gagtgcggcg cggccggcat cgacggtggc ccgctggacg ctcgggtcga tctcgacgag
      181 caggtcgccc tgggcgacct ggctgcccgg ctggacgtgc aggcggacga tctggcccga
      241 gacctgggcg ccgacatcga cgtagcggcg cggttgcagc gtgccgatgg cggtcaccgt
      301 ggcttcgatg tcggcgcgca cgaccggggt cgtctcgtag tcgacgcgat cgcccgcgag
      361 ccaggtggcg acaccgccga ggacgacggc agcggccagc gccgggccga cgccgcggcg
      421 gcgcaggaag gcaggcagcg gcatcagggt tcgatccgga agctgagcgt ggcgacgtgg
      481 cggacgtcgt cgtatttctt gccgtcgacg ctggcgctgc cgttgatccg ggccgtcacc
      541 tcgagcacgt agagggccgg gaaagccggg tcgaagctga ccgaaccgtc ggccgccggc
      601 cgcagcacgc gcgaccagcc ggccgaggtg gcgacgttga cctgcgaggc ggcgaccggg
      661 ctgcccttcc agaacagccg gtaggtgttg ccgcccggcg tcgtcggcac cagttcgagg
      721 tcgctgacgg ccttggtttc ctggcggccg tagcgggcgt ggtagatgac cagcgtcttg
      781 tcgtcgccgc ggcgggcggt gaagcgcagg tcgccggcag cggcggcaac ctggtagccg
      841 tcatcggcgg cgttcagggc gacggccttg ccgtcggcgg cgaaggcgcg ggcgtcgcgc
      901 gctgcggcca gggcctgcgg cttgccgagt tcgccggcga cgatgcggcg gccgccggcg
      961 tcggcgtcga tccagaaata ttcggcgtgc acggtcgacg ccgcgcacag ggcaaaaacc
     1021 ggcagccagc ggcgaccggg caggacggaa agaaagcgtt gcatgcgaaa gcctcgcgga
     1081 aggtcagaga aagaagggcg gcagcgccag cagcgggccg atgacggcca gccaggcggc
     1141 gaagcgcggc gcgtagggca gcaggaagac gaagccgagc gccgccgtcg acagcaggcc
     1201 gaaccaggcg accgggccga tcgcccagcc ccagaccagg ctgcacagcc acagcgccag
     1261 cgccagcgcc agcaggctcc agccggcgag acgcagggcg cggcgcagca gcggcgccgg
     1321 cttgccgtgg cggatctggc tgtggtggcg gtccatcgcc aggctgagcg tgagcaggcc
     1381 ggcgtagcag acgaagaaga ccaggtagag cgcgaagtgc gggtccatct cagcgtcctc
     1441 cgatggcgtc gatgaccgcg gtttttgccg tttccggccg gtcgaccgtc agcgtcgggg
     1501 cgcgccggcg caactggcgc agcatcgcca ggaagatcag gccgaagccg atcgcgctca
     1561 gctcgacgcc ggcggtttcg ccgtcgccgc gggccagggc ggcgaccagc cagtcgccgg
     1621 tggtggccag gttgagcagc ggcagcccca ggcagagcag cgcgcaggcc gccagctgct
     1681 cgcgccaggc ggccagcgac ggacgcagcg cggcgtgcgc caggctggcc agccagaccg
     1741 cgaagaagag cacgatctcg cgcgtgccgc gctcggcgac gtcgagcggg agcagccggt
     1801 tgatccagaa ataagccgcg caggcgagga tcaggccggc gatggcggcg acgttgagca
     1861 cctcgatgcc gcgatagacg cgcgccgtcg cggcgccgaa ctcgttggcc gatttcttgc
     1921 gccgcttgac catgaacagg atcgctccgg tcgccagcat cgccgaggcg gccatgccgc
     1981 agatgaaata cagccacttc atcggatagc cgccgaagtg cgcgaaatgc agcgcgttca
     2041 tcgtccgctg cgtctgcagc acgctgccgc cgcgcaccgt gttcggcagc tgcacgtcga
     2101 gcgtctccag cgtgatcgcg tcgtagagcg cgctgccgct gccgcgcccg accaggcggt
     2161 cgttctcggc gtcggggtcg aagcggccga agacgcgcac ggcggcactg cggtcgccgg
     2221 catgctcgac cacgacgaag ctcgcctcac ggccgatcgc cgcctcgccg cgccgcatca
     2281 gcggcgccag ctcgggcatc ggcgcgtcga tgtgctgcag cggccgcggc gccgggtcct
     2341 cgctcaggcg cgcgaagaag gcctcctcgc tgccgtcgta gcgggcgagg acgccggccg
     2401 gcatgtaggt cgcccagaag atggtcaggc cggtatagga aatcacgaac aggaagggca
     2461 gggtcagtac gccgagcgcg ttgtggccgt cgagccagga gcgctggccc ttgcccgggc
     2521 ggaaggtgaa gaagtcggcg aagatcttct tgtgcacgat gatcccggag accagcgcga
     2581 ccagcatcgc catcgtcgcc gcgccgacga tccagatgcc ggccatgccg gcgtggaact
     2641 cgaagtgcat gtgcacgaaa tggtggccgc cctcggtctc gcggctggca cccggctgca
     2701 gttcggcgcc ggtgagcggg tcgagcagcg cctgcttgaa cggcgcatcc tcgtcggcac
     2761 gccagaacac gcggaggttc gggttttcgt cgccgggcag gccgatcgac cagaaatcgg
     2821 cgcgcggcga caccgccgcc aggtggcgct ggccgagctc gaccgcgcgg ctgcgctggt
     2881 ccacggtcga cggcgcgatt tcgccgattt tcccctgctc gcgcatccag tgcgagatcg
     2941 ggtcgtcgaa gacgccgagc gtgccggtca ggaagatggc gaagaggatc caggtaaacc
     3001 acaggccgcc ccaggtgtgc agccaggaca tcgattgccg cagcgtctgc ttcatgcggc
     3061 ggctccgccg agttgcaggg cggcagccat caggcccatc gccgcgccgg gcaggagcag
     3121 gccgagccag gcgcgggtgg cgctggcggc ggcgaagacc cagatgacgg cggcggtgta
     3181 gacggcgaag ctgaacagcg tcgcgccgag caccgcttcg ggcagctcgg ccggcagcag
     3241 gtagctcagc cagatcgaaa aaacggaagc cagcgcgtag ccgccgacga cggcggcaag
     3301 aacgcgcgac ccgagggcga ggcggggatg ggcgggggat ggaacggaca tggcgaacct
     3361 cggctgttgg gcgtatcggg gaagcgtccg gggcggacgc tgcgcggcac catt
//
LOCUS       DAOSDX010000224         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9785_length_3414_cov_3.369455, whole genome shotgun sequence.
ACCESSION   DAOSDX010000224 DAOSDX010000000
VERSION     DAOSDX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_3414_cov_3.369455"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="PLN02_06090"
     CDS             complement(<1..1500)
                     /locus_tag="PLN02_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOI51929.1"
                     /translation="MKLTVAKKMILLAGAALLGILLLATLNYQQMSKVFDAANYASVN
                     SVPSMATLDAVRSNFARLRIRIDRHVMNTDSTKMADIEQTIKESLKLTEDALKAYASA
                     ISDDRDRAIYEQEKALFAQFVAGIEPVLELSRKNQNEQARELLEKNAAIARQVDQVMN
                     EHFDYNHELAKKAAAEAAATRDSATTLALVIVLLTLATVAFLSIFITRNLLRQLGGEP
                     DFAAEVANKIAAGDLGSKIELKSGDSASLMAAMKHMSETIQRLVGEMNHMSAEHDKGD
                     IDVKIDESSFQGSFKAMAEGTNQMVFGHIAVKKKAMACIREFGEGNFDAPLEAFPGKK
                     KFINDTIEQMRSNLKEFIAQMNRMSREHDAGDIDVRIDEARFRNDFQVMAKGVNEMVF
                     GHIAVKKKAMACVKEFGEGNLEAPLEQFPGKKKFINETIEQLRANLKRIVSEIQDLTA
                     AANRGDFSVKIPLEGKAGFPKTLSELLNQLSDTVDTAFKDTIYVAQALEQ"
     gene            complement(1559..>3414)
                     /locus_tag="PLN02_06095"
     CDS             complement(1559..>3414)
                     /locus_tag="PLN02_06095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOI51930.1"
                     /translation="VVEHATPLTREGGGEGVETDNWHISLRFSRDVLRNGMDPLSFIR
                     YLTTFGEIASIGTLSEDIPRAAEMDPEACYLGFEINYRSTADKATIEGAFEFVQDDAQ
                     VRILPPHSKVSQYLDLILSLPEEDMRVGEILVRCGTLTRHELEQALSAQADARQEGVA
                     GAEIGAVLVARNVVQPAVVEAALQKQKQVKESKAGDAALIRVNADKLDEHINLIGELI
                     IASAGINLAAQGSGQAELLEAASLLNRLVENIRDSALQLRMVQIGATFNKFQRVVRDV
                     SRDLGKDIRLEISGAETELDKTVIEKIGDPLTHLVRNSMDHGIEAADVRLARGKPAHG
                     TLRLNAYHDSGSIVIEVGDDGGGLPKAKILAKAIERGLVKPEQTLSDDEIHALIFEPG
                     FSTADQVNNLSGRGVGMDVVRRNITALRGSIEIDSEEGFGATIRVRLPLTLAIIDGFL
                     VGVGNSSFVVPLEMVVECVELSQDELKAAQGRDQLNLRGEVLPFIRLRELFDMPEPAA
                     PEPTVLAEGMPEVDAELAFLLQPVLRENVVVVNYAGRRAGLVVDALQGEYQTVIRPLG
                     AVFNGLQGVAGFTILGSGQVALILDVPGLVSRVSRRESRRASQINHLAAVD"
BASE COUNT          550 a         1029 c         1118 g          717 t
ORIGIN      
        1 ctgctcgagc gcctgggcaa cgtagatggt gtccttgaac gcggtatcga ccgtgtcgga
       61 tagctggttg agcagttcgg acagggtctt cgggaagccg gccttgcctt cgagcgggat
      121 cttcaccgag aaatcgccac ggttggcggc agcggtgaga tcctgaattt cgctgacgat
      181 gcgcttgaga ttggcacgca actgctcgat ggtctcgttg atgaacttct tcttgcccgg
      241 gaattgctcc agcggcgctt caaggttgcc ctcaccgaat tccttgacac aggccatcgc
      301 cttcttcttg accgcgatgt ggccgaagac catctcgttg acacccttgg ccatgacctg
      361 gaaatcgttc ctgaagcgcg cttcgtcgat cctgacgtcg atgtcgccgg cgtcgtgctc
      421 gcgcgacatg cggttcatct gggcaatgaa ctccttcaga ttgctgcgca tctgttcgat
      481 ggtgtcattg atgaatttct tcttgcccgg gaaagcctcc agcggcgcat cgaagttgcc
      541 ttcgccgaat tcccggatgc aggccatcgc cttcttcttc accgcgatgt ggccgaagac
      601 catctggttg gtgccctcgg ccatcgcctt gaacgagccc tggaagctgc tctcgtcgat
      661 cttgacgtcg atgtcgccct tgtcgtgttc ggcggacatg tggttcatct cgccgaccag
      721 acgctggatc gtctcgctca tgtgtttcat tgccgccatc aggctagcgc tgtcgccgct
      781 cttcaattcg atcttgctgc ccaggtcacc ggcggcgatc ttgttcgcca cttcggccgc
      841 gaagtccggc tcgccaccca actggcgcag caggttgcgg gtgatgaaga tgctcaggaa
      901 ggcaaccgtc gccagcgtca gcaggacgat gacaagggcc agggtggtgg cgctgtcgcg
      961 ggtcgcggca gcttcggcgg cggccttctt ggccagctcg tggttgtagt cgaaatgctc
     1021 gttcatcacc tggtctacct gacgggcgat ggcggcgttc ttctcgagca gttcgcgggc
     1081 ctgttcgttc tggttcttgc gcgacagttc gaggacgggc tcaatgccgg cgacgaactg
     1141 ggcaaagagc gccttttcct gttcgtaaat ggcccgatcg cggtcgtcgg aaatcgccga
     1201 cgcatatgct ttcagggcat cttccgtcag tttcaggctt tccttgatcg tctgctcgat
     1261 gtccgccatc ttggtcgagt ccgtgttcat gacgtggcga tcgatgcgga tgcgcaggcg
     1321 ggcgaaattc gacctgaccg catccagcgt ggccatactg ggcactgaat tgaccgaggc
     1381 gtagttggcg gcgtcgaaca ccttgctcat ctgctggtag ttgagcgtgg ccagcagcag
     1441 gatgccgagc aacgcggcgc cagcaagcaa gatcattttc ttggcgacgg tgagtttcat
     1501 gagagtctcc gattcagaaa cggtggggtg agttgttggt ggagtaagga ctggccgctc
     1561 aatcgacagc ggccaggtgg ttgatctggc tggcgcgccg gctttcccgg cgactgaccc
     1621 ggctgaccag gccgggaaca tcaaggatca gggcgacctg gccgctgccc agaatggtga
     1681 aaccggcgac gccctgcagg ccgttgaaga cggcgccgag cgggcggatc acggtctggt
     1741 attcgccctg cagcgcatcg acgacgaggc cggcacgacg ccccgcataa ttgacgacga
     1801 cgacgttttc gcgcagcacc ggttgcagca ggaaggccag ttccgcgtcg acctcgggca
     1861 tcccttcggc cagcaccgtc ggctccggcg cggccggttc gggcatgtcg aagagttcgc
     1921 gcagacggat gaagggcagc acttcgccgc gcaggttgag ctggtcgcgc ccctgcgccg
     1981 ccttcaactc gtcctgagag agttcgacac attcgacgac catctccagc ggcacgacga
     2041 aggacgagtt gccaacaccg accaggaaac cgtcgatgat cgccagcgtc agcggcagac
     2101 ggacgcggat cgtcgcgccg aagccttcct cgctgtcgat ctcgatgctg ccgcgcagcg
     2161 cggtgatgtt gcggcgcacg acgtccatgc cgacgccacg cccggacagg ttgttgacct
     2221 gatcggcggt ggagaagcca ggttcgaaga tcagcgcgtg gatttcgtcg tcgctcagtg
     2281 tctgctccgg cttgaccagg ccgcgttcga tcgccttggc gaggatcttg gccttgggca
     2341 ggccaccgcc gtcgtcgccg acctcgatga cgatgctgcc cgaatcgtgg taggcgttca
     2401 ggcgcaacgt gccgtgcgcc ggcttgccgc gggcgaggcg gacgtcggcg gcttcgatgc
     2461 cgtggtccat cgagttgcgc acgagatggg tcagcgggtc gccgatcttc tcgatcacgg
     2521 tcttgtcgag ctcggtttcg gcgccggaga tttccaggcg gatgtccttg ccgagatcgc
     2581 gcgagacgtc gcggacgacg cgctggaatt tattgaaagt ggcgccgatc tgcaccatgc
     2641 gcaattgcag cgccgaatcg cggatgttct cgacaaggcg attgagcagc gatgccgcct
     2701 ccagcagttc ggcctggccg ctgccctggg cggcgaggtt gatgccggcg ctggcgatga
     2761 tcagttcgcc gatcaggttg atgtgttcgt cgagcttgtc ggcattgacg cggatcagcg
     2821 ccgcgtcacc ggccttgctt tccttgacct gcttctgttt ctgcagcgcg gcttcgacca
     2881 ccgccggctg gaccacgttg cgggcgacca gcacggcgcc gatttcggcc ccggcaacgc
     2941 cttcctggcg cgcgtcggcc tgagcgctga gcgcctgttc cagttcgtgg cgggtcaagg
     3001 tgccgcagcg gacgaggatt tcgccgacgc gcatgtcctc ttcgggcagg ctgaggatca
     3061 ggtcgaggta ttgcgagacc ttgctgtgcg gcggcaggat gcgcacctga gcgtcgtcct
     3121 ggacgaattc gaaggcgcct tcgatggtcg ccttgtcggc cgtgctgcgg tagttgatct
     3181 cgaaaccgag gtagcaagct tcggggtcca tctcggcggc gcgcggaatg tcctcgctca
     3241 gggtgccgat cgaggcaatc tcgccgaagg tcgtcaggta gcgaatgaaa gacagcgggt
     3301 ccatgccgtt cctgaggaca tcccgggaga agcgcagcga gatgtgccag ttgtcggtct
     3361 cgacaccttc gccgccgcct tcgcgggtca gcggcgtggc gtgctcgacg acct
//
LOCUS       DAOSDX010000225         3413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9791_length_3413_cov_3.141751, whole genome shotgun sequence.
ACCESSION   DAOSDX010000225 DAOSDX010000000
VERSION     DAOSDX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9791_length_3413_cov_3.141751"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLN02_06100"
     CDS             <1..378
                     /locus_tag="PLN02_06100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOI51931.1"
                     /translation="KLGGGVRLALAQYRELAAFAQFASDLDEATRKQLERGRLVTELM
                     KQPQYAPMSISEMAVTLYAADKGYFDDVEVKRALECEKAMISYLKANHAEVMNTMESS
                     ADLSGDTEKALAAGIQAFKSTWA"
     gene            394..>803
                     /locus_tag="PLN02_06105"
     CDS             394..>803
                     /locus_tag="PLN02_06105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOI51932.1"
                     /translation="MPSGKEIRNKIKSVENTRKITKAMEMVAASKMRKAQDRMRAARP
                     YGEKIRRVASNLSHALTEYKHPFLEKREPKAIGIVLVTSDKGLCGGLNSNVLRLVLGK
                     MKELDSQQIKFQATCIGNKGLGFMQRAGAKVVSHV"
     assembly_gap    804..813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <814..1267
                     /locus_tag="PLN02_06110"
     CDS             <814..1267
                     /locus_tag="PLN02_06110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOI51933.1"
                     /translation="VALGDTPHLEKLIGPLKIQLDAYMKGEIDALYIGYTRFINTMKQ
                     EPVFERLLPLADDVVKTDHQPGWDYVYEPEAQPVIDDLLIRYVEALIYQAVAENMASE
                     QSARMVAMKSASDNAKNVIGELKLVYNKARQAAITKELSEIVSGAAAV"
     gene            1305..2708
                     /gene="atpD"
                     /locus_tag="PLN02_06115"
     CDS             1305..2708
                     /gene="atpD"
                     /locus_tag="PLN02_06115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOI51934.1"
                     /translation="MSQGSIVQCIGAVVDIHFPRDAMPKVYDALKLDASEENGMVETG
                     LTFEVQQQLGDGVVRTIAMGSSDGLRRGMKVNNTGAGISVPVGMGTLGRIMDVLGRPI
                     DEAGPIDSNELREIHAAVPKFDELSSSVDLLETGIKVIDLICPFAKGGKVGLFGGAGV
                     GKTVNMMELINNIAKQHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVFGQMNE
                     PPGNRLRVALTGLTMAERFRDDGRDILFFVDNIYRYTLAGTEVSALLGRMPSAVGYQP
                     TLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFLHLDSTVVLSRDIAS
                     LGIYPAVDPLDSTSRQLDPQVVGEEHYGVARAVQMTLQRYKELRDIIAILGMDELSPE
                     DKLAVSRARKIQRFLSQPFHVAEVFTGSPGKFVPLKETIKGFKGIVSGEYDHLPEQAF
                     YMVGGIEEAVEKAKTLQ"
     gene            2734..3159
                     /locus_tag="PLN02_06120"
     CDS             2734..3159
                     /locus_tag="PLN02_06120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOI51935.1"
                     /translation="MVMTVHCDVVSAEESIFSGLVEIAVFPGEAGELGILPQHTPLLT
                     RIKPGTIRLKVPEQSEFELVYVSGGMLEVQPDMITVLADTAIRAHDLDEAKALEAKKR
                     AEEALANRQAEMDYAAAEAELAEAIAQLQAIQRLRKHTH"
     gene            complement(3206..>3413)
                     /locus_tag="PLN02_06125"
     CDS             complement(3206..>3413)
                     /locus_tag="PLN02_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51936.1"
                     /translation="RIFSVRPATLPASPATTALRILKDDQAPGLRKTIHGGILTGFAR
                     RTQPGEANSPIAPCAMARRCRSQK"
BASE COUNT          715 a         1054 c         1011 g          623 t
ORIGIN      
        1 aagctcggcg gcggcgtgcg tctggcgctg gcgcagtacc gcgaactcgc ggccttcgcc
       61 cagttcgcct ccgacctcga cgaagcaacc cgcaagcagc tggaacgcgg ccgtctggtc
      121 acggaactga tgaagcagcc gcagtacgcc ccgatgagca tctccgagat ggctgtcacc
      181 ctttacgccg ccgacaaggg ctacttcgac gacgtcgaag tcaagcgcgc gctcgaatgc
      241 gaaaaggcca tgatcagcta cctcaaggcc aatcacgccg aagtcatgaa taccatggaa
      301 tcgtcggccg acctgagcgg tgataccgaa aaggcgctgg ctgccggcat ccaggctttc
      361 aagagcacct gggcctgatc ttaggagaac gccatgccta gcggcaagga aattcgcaac
      421 aagatcaaga gcgtagaaaa tacgcgcaag atcaccaagg ccatggagat ggtggctgca
      481 tccaaaatgc gcaaggcgca ggaccggatg cgggccgccc gcccctacgg cgagaagatc
      541 cggcgcgtgg ccagcaactt gtcgcatgcg ctgaccgagt acaagcatcc cttcctcgag
      601 aagcgcgagc cgaaggcgat cggcattgtc ctcgtcacct ccgacaaggg tctttgcggt
      661 ggcttgaact ccaacgtcct gcgcctggtc ctgggcaaga tgaaggagct ggacagccag
      721 cagatcaagt tccaggcaac ctgcatcggc aacaagggtc tgggtttcat gcaacgcgcc
      781 ggcgcgaagg ttgtctcgca cgtnnnnnnn nnncgtcgcg ctgggtgata ccccgcacct
      841 ggaaaagctg atcggcccgc tcaagatcca gctcgacgcc tacatgaagg gcgaaatcga
      901 cgcgctgtac atcggctaca cccgcttcat caacacgatg aagcaggaac cggttttcga
      961 aaggctgctc ccgctcgctg acgacgtcgt caagaccgat catcagccgg gttgggacta
     1021 tgtctacgag ccggaagccc agccagttat cgacgatctg ttgatccgct atgtcgaagc
     1081 tttgatttac caggccgtgg ctgaaaacat ggcctccgag cagtccgccc ggatggtcgc
     1141 catgaagtct gcttccgata acgccaagaa cgtcatcggt gaattgaagc tggtctataa
     1201 caaggcccgt caggccgcga ttaccaagga actctcggaa atcgtcagcg gcgccgccgc
     1261 agtctgacgc cagcgcgcag cttttaatta ttggggattt gaatatgagt caaggttcca
     1321 tcgttcagtg tatcggcgca gttgtggaca tccacttccc gcgcgacgcg atgccgaagg
     1381 tctacgacgc actcaagctc gacgcctccg aagaaaacgg catggttgaa accggcctga
     1441 ccttcgaagt gcagcagcaa ctgggtgacg gcgtcgtccg taccatcgcc atgggttcat
     1501 ccgacggcct gcgccgcggc atgaaggtta acaacaccgg tgccggcatc tcggtgccgg
     1561 tcggcatggg cacgctgggt cgcatcatgg acgtgctcgg ccgtccgatc gacgaagctg
     1621 gcccgatcga cagcaacgag ctgcgtgaaa tccacgccgc cgtgccgaag ttcgacgaac
     1681 tgtcgtcttc cgtcgatctg ctcgaaaccg gcatcaaggt tatcgacctc atctgcccgt
     1741 tcgccaaggg cggcaaggtc ggcctgttcg gcggcgccgg cgtcggcaag accgtcaaca
     1801 tgatggaact gatcaacaac atcgccaagc aacactcggg tctgtccgtg tttgccggtg
     1861 ttggtgagcg tacccgcgaa gggaacgact tctatcatga aatgaaggac tccaacgttc
     1921 tcgacaaggt cgcgatggtg ttcggtcaga tgaacgaacc cccgggcaac cgtctgcgcg
     1981 tcgcgctgac cggtctgacc atggccgagc gtttccgtga cgacggtcgt gacatcctgt
     2041 tcttcgtcga taacatctac cgctacacgc tggccggtac cgaagtgtcc gcactgctcg
     2101 gccgcatgcc ttccgcggtg ggctatcagc cgacgctggc cgaagaaatg ggccgtctgc
     2161 aagagcgcat cacctcgacc aaggtcggct cgatcacctc gatccaggcc gtgtatgtgc
     2221 ctgccgatga cttgaccgat ccgtcgccgg ccaccacctt cctgcacttg gactccacgg
     2281 tcgtgctgtc gcgtgacatc gcctcgctgg gtatctaccc ggccgtcgat ccgctcgact
     2341 ccacctcgcg ccagctcgac ccgcaagtcg tcggcgaaga gcactacggt gttgcccgtg
     2401 ccgtgcagat gacgctgcag cgctacaagg aactgcgtga catcatcgcg attctgggta
     2461 tggacgaact gtcgccggaa gacaagctgg ccgtcagccg cgcccgcaag atccagcgct
     2521 tcctgtcgca gcccttccac gttgccgaag tgttcaccgg ttccccgggc aagttcgttc
     2581 cgctcaagga aaccatcaag ggcttcaagg gcatcgtcag cggcgagtac gatcacctgc
     2641 cggaacaggc cttctacatg gtcggcggca tcgaagaagc tgtcgaaaag gcgaagacgc
     2701 tgcagtaatg cggcctcagc ataggagcca gtaatggtta tgaccgttca ttgtgatgtc
     2761 gtcagtgccg aagaatcgat tttctccggc ctggtggaaa tcgcagtgtt ccccggcgaa
     2821 gccggggaac tcggcatcct gccgcaacat accccgctgt tgacccgcat caagcccggc
     2881 accattcgtc tgaaggtgcc ggagcagagc gagttcgaac tggtgtatgt ctccggcggc
     2941 atgctggaag tccagcccga catgatcacc gtgttggccg acaccgcgat tcgtgcccat
     3001 gacctcgacg aggccaaggc actggaagcc aagaagcgcg ccgaggaagc actggccaat
     3061 cgtcaggcgg agatggatta cgccgctgcg gaagccgaac tggccgaagc cattgcccag
     3121 ttgcaggcga ttcagcgtct gcgcaagcac acccactgaa acgggcaaca tgtcccgcac
     3181 aaagagcagc ctacgggctg ctttttcact tttgcgaccg gcatcggcgc gccatcgcgc
     3241 acggcgcgat cggcgaattc gcctcgccgg gctgtgtccg gcgggcgaat cctgtcagaa
     3301 tgccgccatg gattgttttt ctcaacccgg gcgcctgatc gtccttcagg atacggagcg
     3361 ctgtcgtggc tggtgatgcc ggtaaggtcg cgggccggac tgaaaaaatg cgc
//
LOCUS       DAOSDX010000226         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9803_length_3410_cov_3.380030, whole genome shotgun sequence.
ACCESSION   DAOSDX010000226 DAOSDX010000000
VERSION     DAOSDX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9803_length_3410_cov_3.380030"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLN02_06130"
     CDS             complement(<1..303)
                     /locus_tag="PLN02_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51937.1"
                     /translation="MTRHFIPTLLSVTLAAACIASASANPAPPAPTTASVTDAWCDDL
                     GARLHSVDADHCRKQSFVTGSERTAGGRALVWRDIKAVSKHPDKAPRVLVVGGIHGD"
     gene            453..1739
                     /gene="serS"
                     /locus_tag="PLN02_06135"
     CDS             453..1739
                     /gene="serS"
                     /locus_tag="PLN02_06135"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOI51938.1"
                     /translation="MLDIQQLRSNLDAVAASLAKRGKPIDFTAFAALEAERKTLQTRT
                     QDLQAQRNSLSKQIGMLKGQGKDASEVMAQVGALGDELKASEARLAELLGEFNAILAA
                     LPNIPDESVPVGADESANVEIKRWGTPRVFDFPVKDHTDVGEAIGQLDFATAAKISGA
                     RFSLLKGGLARLHRALAQFMLDTHTAEHGYTELYAPYLVNAASLYGTGQLPKFEEDLF
                     KVLRGEQDPLYLIPTAEVPVTNIVRDEILAAEVLPLKFVCHTPCFRSEAGSGGRDVRG
                     MIRQHQFDKVELVQIVHPEKSAEAHEELTRHAEVILEKLELPYRRVVLCTGDMGFSAA
                     KTYDLEVWLPAQDTYREISSCSNFGAFQGRRMQARFRNDKNKPEICHTLNGSGLAVGR
                     TLVAILENNQRADGSVEIPAALRPYMGGLESLTPTR"
     gene            complement(1914..3335)
                     /gene="mltF"
                     /locus_tag="PLN02_06140"
     CDS             complement(1914..3335)
                     /gene="mltF"
                     /locus_tag="PLN02_06140"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HOI51939.1"
                     /translation="MARFNSLVAFLLTLLLAACDRGWPVPLPYPTPGKQNLVVLTRPG
                     PLTYQLDESGSISGLEYDLVNGLAEELGVPVKFHVLSPGELSRQLLNGTYHIAASWLS
                     PGNNPEMQSGPPLFQTHDVLAQHEASLPLTSLDQLAGKTVHALAGTRQAATIRRLMEK
                     IPGLQLIEIGQGDVIDLLEALGEQKISYVAMDGKFADIANQYVPSLRTTLPLSSEAPV
                     VWWLGPVPNPELKARIEAYLERIRADGTLARLEERYFGHVRRLQQDDVEKFLGHIKTT
                     LPQLRKHFLDAERQTGIDWRLIAALAWQESHWDPFATSYTNVRGLMMLTEETADRLGV
                     SNRLDPRESVLAGARYLNMIRNSLPHDMPEPDRTWLAIAGYNIGPGHLNAARTIGKQL
                     NADPGAWYDMKRVLPLLAKPKYYSRLKSGRARGGEAVIMVENIRSYYDILVRNAPPLT
                     AAISPMDGKIGMRGEAPELRLGR"
BASE COUNT          573 a         1060 c         1152 g          625 t
ORIGIN      
        1 gtcgccgtgg atgccgccga cgaccagcac gcgcggtgcc ttgtccgggt gcttgctgac
       61 cgccttgatg tcgcgccaga ccagggcgcg accgccagcg gtacgctcgc tgccggtgac
      121 gaaggactgc ttgcggcagt ggtcggcgtc gacgctgtgc aggcgggcgc caaggtcgtc
      181 gcaccaggcg tcggtgacgc tggcggtggt tggggcgggg ggggctgggt tggctgaggc
      241 ggaagcgatg caggcggcgg caagtgtcac ggaaagcagg gttgggatga aatggcgagt
      301 catgcgtttt gaatatttat gagcgaggcg taatgataac cgggtcagtc cgattctggg
      361 cgttggcgtg tcgcgtataa tcaccggctg atttggcgcc ggctgattcc aggggaatcc
      421 gctgccgcct ggccccatcc aggaaaatga tcatgctcga catccaacaa ctccgttcca
      481 atctcgatgc cgtcgccgcc agtctggcca agcgcggcaa acccatcgac ttcaccgcct
      541 tcgcggcgct ggaagccgag cgcaagacgc tgcagacgcg tacccaggac ctgcaggcgc
      601 agcgcaacag cctgtccaag cagatcggca tgttgaaagg gcagggcaag gatgcgtccg
      661 aggtgatggc ccaggtcggt gcgctgggcg acgaactgaa ggcgtccgaa gcgcgcctgg
      721 ccgaattgct cggcgagttc aacgcgatcc tcgccgcgct gcccaacatc cccgacgaat
      781 cggtgccggt tggcgccgac gagagcgcca acgtcgagat caagcgctgg ggcacgccgc
      841 gcgttttcga cttcccggtc aaggaccata ccgatgtcgg cgaggccatc ggtcagctcg
      901 atttcgccac cgccgccaag atttccggcg cccgcttctc gctgctgaag ggcggtctgg
      961 cccgtctgca ccgcgcactc gcccagttca tgctcgacac gcataccgcc gagcacggct
     1021 acaccgagct ctacgcgccc tacctggtca acgctgccag cctctacggt accggccagc
     1081 tgccgaagtt cgaggaagac ctgttcaagg tcctgcgcgg cgagcaggac ccgctctacc
     1141 tgattccgac cgccgaagtg ccggtgacca acatcgtccg cgacgaaatc ctcgccgccg
     1201 aggtgctgcc gctcaagttc gtctgccaca ccccgtgctt ccgttccgaa gccggctccg
     1261 gcgggcgcga cgtgcgcggc atgatccgcc agcaccagtt cgacaaggtc gagttggtgc
     1321 agatcgtcca tccggaaaaa tcggccgaag cgcacgagga actgacccgc cacgccgaag
     1381 tcatcctcga aaaactggaa ctgccttacc gtcgcgtcgt gctgtgtacc ggcgacatgg
     1441 gcttctccgc cgccaagacc tacgacctcg aagtctggct gccggcgcag gacacctacc
     1501 gcgagatttc ctcctgctcc aacttcggcg ccttccaggg ccgccgcatg caggcgcgct
     1561 tccgtaacga caagaacaag ccggaaatct gccacaccct gaacggctcc ggtctggcgg
     1621 ttggtcgcac gctggtggcg attctggaaa acaatcagcg cgccgacggc agcgtcgaaa
     1681 tcccggccgc tttgcgcccc tacatgggcg gtttggaaag cctgacgccg acgcgctgat
     1741 tgtttcggcg caggctaacc gcgcgtagcg cgcgttaagc gccgcaggcg cggccgtagc
     1801 caaaacaatt ggttggcgcg ccgacggcag cgtcgaaatt ccggtagctt tgcctcccta
     1861 catggggggg ctggaacagc tgacgtcgac gcgctgatcg ttgcggcgcc ggttcagcgt
     1921 ccgagcctga gttccggcgc ctcgccgcgc atgccgatct tgccgtccat cggcgagatg
     1981 gcggcggtca gcggtggcgc gttgcggacc aggatgtcgt aatagctgcg gatgttttcg
     2041 accatgatca ccgcttcgcc gccgcgcgcc cggccggatt tgagccggct gtaatacttg
     2101 ggcttggcca gcagcggcag gacccgcttc atgtcgtacc aggcgccggg gtctgcgtta
     2161 agctgcttgc cgatggtccg tgccgcgttg aggtggccgg ggccgatgtt gtagccggcg
     2221 atcgccagcc aggtgcggtc gggttccggc atgtcgtgcg gcaggctgtt gcggatcatg
     2281 ttcaggtagc gggcgccggc caggacgctc tcgcgcgggt cgaggcggtt gctgacgccg
     2341 agccggtcgg cggtttcctc ggtcagcatc atcaggccac ggacattggt atagctggtg
     2401 gcgaacgggt cccagtgcga ttcctgccag gccagcgcgg cgatcagccg ccagtcgatg
     2461 ccggtctgcc gttccgcatc caggaaatgc ttgcgcaact gtggcagcgt ggtcttgatg
     2521 tggccgagga atttttcgac gtcgtcctgc tgcaggcggc ggacgtggcc gaaatagcgt
     2581 tcttctaggc gggccagcgt gccatcggcg cggattcttt cgagataggc ttcgatccgc
     2641 gccttcagtt ccggattcgg caccggcccc agccaccaga cgaccggcgc ctcgctgctc
     2701 agcggcaggg tggtgcgcag gctgggaaca tactggttgg cgatgtcggc gaacttgccg
     2761 tccatcgcga cgtaggaaat cttctgttcg ccgagcgctt cgagcaggtc gatgacgtcg
     2821 ccctgaccga tttcgatcag ttgcaggccg gggatttttt ccatcagacg gcggatcgtt
     2881 gctgcctgcc gggttccggc cagcgcgtgc acggtcttgc cggccagttg atcgagcgag
     2941 gtcagcggca gcgaagcttc gtgctgggcg aggacgtcgt gggtttggaa gagtggcggg
     3001 ccgctctgca tctccggatt gtttcccggc gacagccagg aggcggcgat gtggtaggtg
     3061 ccgttgagca actgccggct gagttcgccg ggtgacagaa cgtggaattt gaccgggacg
     3121 cccagttcct cggccaggcc gttgaccaga tcgtattcca gcccgctgat gctgcccgat
     3181 tcgtcgagct ggtaggtgag cgggccgggg cgcgtcaaca cgacgaggtt ctgcttgccc
     3241 ggcgtcgggt agggaagcgg taccggccat cccctgtcgc aggcagcgag cagcagagtg
     3301 agcagaaatg cgacaaggct gttaaaccgc gccataactt gtgctattat ctcggccgct
     3361 gcggagaggt ggcagagtgg tcgaatgtac ctgactcgaa atcaggcgta
//
LOCUS       DAOSDX010000227         3400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9839_length_3400_cov_2.746487, whole genome shotgun sequence.
ACCESSION   DAOSDX010000227 DAOSDX010000000
VERSION     DAOSDX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_3400_cov_2.746487"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..645
                     /locus_tag="PLN02_06145"
     CDS             25..645
                     /locus_tag="PLN02_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOI51940.1"
                     /translation="MFRRLIERVFPKMPDFFAMLTLQCRHVAQTAGLLVEFMETAAPD
                     IGRQIRKDEHEADRVKIHNLHALNEAFSTPIDREDLYRAIMDLDEVVNYCKTTVSEME
                     VLQLDPDKHCLEMAMHIKLGTDALLQGFTRLAREPAQAAADADIARKAERRVEKAYRR
                     AIADLFQGDDYIHMFKRREIYRHLSNAADRMAHCANTLHDIVVKMC"
     gene            complement(649..864)
                     /locus_tag="PLN02_06150"
     CDS             complement(649..864)
                     /locus_tag="PLN02_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOI51941.1"
                     /translation="MSLNFAVRGEHIQLDQLLKATGLCESGGAAHAAIADGQVRVDGV
                     VDTRKRAKIRPGQRVDFAGQTVLVTGE"
     gene            complement(861..1511)
                     /locus_tag="PLN02_06155"
     CDS             complement(861..1511)
                     /locus_tag="PLN02_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HOI51942.1"
                     /translation="MSARDEILARVRAGLAKGDAGARRTAALAYLRTASPGPQPTLAG
                     DLLTRFCAKAGSLASTVERVADYAAVPARVAAYLAAHDLPARAVTALDVAGLDWAAAG
                     IVSHSGSAVDADPVGITGCFCAIAETGTLLLLSGPATPASASLLPETHIALVPAARVV
                     ATMEDAFALLHRERGQLPRAANFVSGPSRTGDIEQTIVLGAHGPCRVHLIIIEEES"
     gene            complement(1508..2464)
                     /locus_tag="PLN02_06160"
     CDS             complement(1508..2464)
                     /locus_tag="PLN02_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOI51943.1"
                     /translation="MLCIVHLDRASVVADFRRPAFAHDWIDYPQTAPQQVVERLRGAS
                     IAITNKVPLSAAVIDALPELRMIAVAATGTNIIDLDACRRRGIVVSNIRGYAEHTVPE
                     HVFALLLALSRNLFAWRATIQAGAWQAAEQFCLFDHPIRDLHGATLGVIGAGSLGNGV
                     VRLGAAFGMRVLRAERKDAASVRPGYTAFAEVLAAADAISLHCPLSDATRHLIGEAEL
                     RAMKSSALLINTARGGIVDEAALARALREGWIAGAGFDVLSVEPPPDDQPLLDPALLA
                     QPNFLLTPHVAWSSRPAMQMLAEQLVDNLEAFVAGAPRNVVT"
     gene            complement(2464..3159)
                     /locus_tag="PLN02_06165"
     CDS             complement(2464..3159)
                     /locus_tag="PLN02_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOI51944.1"
                     /translation="MEFAPEGPMAIPLWINGRPYLTVGDCFFDVVNPATGQARHRVPM
                     CGAEEAAEAVAAARDAQAAWAGLGLAGRQECLARLADGLEKYAGHFAKLLVQESACPE
                     AQADAEVAAALVALREASVGETGVAALVVAAGQPLASLAAAAAPALLAGATLVVKPSP
                     QAPAAAFALCELATRCDWPGGVLNLVHGDGPAIEGLCAAGVDCLIYSGDAALGAKVGE
                     LAAAHGTPFVTAA"
     gene            complement(3162..>3400)
                     /locus_tag="PLN02_06170"
     CDS             complement(3162..>3400)
                     /locus_tag="PLN02_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3390 domain-containing protein"
                     /protein_id="HOI51945.1"
                     /translation="LRPWGERLGLRAWAWVAGRPALYRALTRFAARYLNWLADGEGRI
                     RIFGFAPGWTAARDLPVAAGGKTFFEQYAARKRD"
BASE COUNT          553 a         1215 c         1159 g          473 t
ORIGIN      
        1 agcgacaaca ccggttcgcc ctcgctgttc cgccggttga tcgagcgcgt ctttcccaag
       61 atgcccgact ttttcgccat gctcaccctg caatgccggc atgtcgcgca gaccgccggc
      121 ctgctggtcg agttcatgga aaccgccgcc cccgacatcg gcaggcagat ccgcaaggac
      181 gagcacgaag ccgaccgggt caagatccac aacctgcacg cgctgaacga ggccttttcg
      241 acgccgatcg accgcgagga cctttatcgg gcgatcatgg acctcgacga agtcgtcaat
      301 tactgcaaga ccacggtcag cgaaatggaa gtcctgcagc tcgaccctga caagcattgc
      361 ctggaaatgg cgatgcacat caagctgggc accgacgccc tgctccaggg cttcacccgg
      421 ctggccaggg agccggcaca ggcggccgcc gacgccgaca tcgcgcgcaa ggccgagcgc
      481 cgcgtcgaaa aggcctaccg gcgggcgatc gccgacctct tccagggcga cgactacatc
      541 cacatgttca agcgccggga aatctaccgc catctttcca acgccgccga ccgcatggcg
      601 cactgcgcca acacgctgca cgacatcgtc gtcaagatgt gctgagcgct actcgccggt
      661 aacgaggacc gtctgaccgg cgaaatcgac ccgctggccg ggccggatct tggcccgctt
      721 gcgggtatcg acgacgccat cgacgcggac ctgcccgtcg gcgatcgccg catgcgctgc
      781 gccgcccgat tcgcaaagac cggtcgcctt gagcaactgg tcgagctgga tgtgctcgcc
      841 gcgcacggca aaattgagac tcatgattct tcctcgatga tgatcaggtg cacccggcac
      901 ggcccgtggg cgccgaggac gatggtttgt tcgatgtcgc cggtgcgcga cggcccggaa
      961 acgaagttgg ccgcccgcgg caactgcccg cgctcgcggt gcagcagcgc gaaggcgtct
     1021 tccatcgtcg ccaccacccg tgccgccggc accagcgcga tgtgggtttc cggcagcagg
     1081 ctggccgacg ccggcgttgc cgggccggac agtaacagca aggtgccggt ttcggcgatg
     1141 gcgcagaagc agccggtgat gccgaccgga tcggcatcca ccgcactgcc gctgtggctg
     1201 acgataccgg cggcggccca gtcgaggccg gcgacgtcga gcgcggtcac cgcccgcgcc
     1261 ggcagatcat gtgcggccag gtaggcggcg acgcgcgccg gtaccgcggc gtagtcggca
     1321 acccgctcga ccgtgctcgc caggctgccc gccttggcgc agaagcgcgt cagcagatcg
     1381 ccggccagcg tcggctgcgg ccccggcgac gccgtccgca ggtaggccaa ggcggcggtg
     1441 cggcgcgcac cggcatcgcc cttggcgagc ccggcacgga cgcgggcgag gatctcgtcg
     1501 cgcgcgctca tgtcacgaca ttcctcggcg cgccggcgac gaaggcttcg aggttgtcca
     1561 ccagctgctc ggccagcatc tgcatcgccg gccgactgga ccacgcgaca tgcggcgtca
     1621 gcaggaaatt gggttgcgcc agcaaggccg gatcgagcag tggctgatcg tccggcggcg
     1681 gttcgacgct cagcacgtcg aagccggcac cggcaatcca gccttcgcgc agcgcccggg
     1741 ccagcgcggc ttcgtcgacg atgccgccgc gcgccgtgtt gatcagcagc gccgaagatt
     1801 tcatcgcccg cagttcggcc tcgccgatca ggtggcgggt ggcgtcgctg agcgggcaat
     1861 gcaggctgat cgcgtcggcc gccgccaata cctcggcgaa agcggtgtag ccggggcgca
     1921 cgctggcggc atccttgcgt tcggcgcgca gcacgcgcat gccgaaggct gcgccgaggc
     1981 gaacgacacc gttgccgagg ctgccggcgc cgatgacgcc gagcgtcgcg ccgtgcaggt
     2041 cgcggatcgg gtggtcgaac aggcagaact gttcggcggc ctgccaggcg ccggcctgga
     2101 tcgtcgcgcg ccaggcgaag aggttgcgcg acagggccag gagcagcgcg aagacatgct
     2161 cgggaaccgt atgttcggcg tagccgcgaa tgttgctgac gacgatgccg cgccgccgac
     2221 aggcgtcgag atcaatgatg ttggtaccgg tggcggcgac cgcgatcatc cgcaactcag
     2281 gcagcgcgtc gatcaccgct gccgacagcg gcaccttgtt ggtgatggcg atgctggcgc
     2341 cgcgcaggcg ctcgaccacc tgctgcggcg ccgtctgcgg gtagtcgatc cagtcgtggg
     2401 cgaaggccgg ccgccggaag tcggcgacca ccgacgcccg atcgagatgg acgatgcaca
     2461 acatcaggcc gccgtcacga agggcgtgcc gtgcgccgcc gccagctcgc caaccttggc
     2521 gccaagcgcg gcgtcaccgc tgtagatcag gcaatcgacg ccggccgcgc acagcccctc
     2581 gatcgccggc ccgtcgccgt gcaccaggtt gagcaccccg cctggccagt cgcagcgcgt
     2641 tgccagttcg cacagcgcga aggcggccgc cggcgcctgc ggactcggct tgacgaccag
     2701 cgtcgccccc gccagcaagg cgggcgctgc tgccgctgcc aggctcgcca gcggctggcc
     2761 ggcagcgacg accagcgcgg cgacaccggt ttcgccaacg ctcgcctcac gcaacgcgac
     2821 aagcgccgct gccacctcgg cgtccgcctg tgcttccggg caggcgctct cctgcaccag
     2881 caacttggcg aaatggccgg cgtacttctc caggccgtcg gcgaggcggg ccaggcattc
     2941 ctggcgaccg gccagaccga gcccggccca ggcggcctgc gcgtcgcgcg ccgccgccac
     3001 cgcctcggcc gcttcctcgg cgccgcacat cggcacccgg tgacgggcct gcccggtcgc
     3061 cggattgacc acatcgaaga aacagtcgcc caccgtcagg taggggcggc cgttgatcca
     3121 gaggggaatg gccatcgggc cttcgggtgc gaattccatc gtcagtctcg ctttcttgct
     3181 gcgtattgtt cgaaaaaggt cttgccgccg gccgccaccg gcagatcgcg cgctgccgtc
     3241 cagcccggcg caaaaccgaa gatgcggatg cgcccctcgc cgtcggccag ccagttcagg
     3301 tagcgcgcgg cgaagcgggt cagcgcacgg tacagcgccg gccggccggc gacccaggcc
     3361 caggcgcgca gcccgaggcg ttcgccccag gggcgcaggt
//
LOCUS       DAOSDX010000228         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9860_length_3395_cov_3.618563, whole genome shotgun sequence.
ACCESSION   DAOSDX010000228 DAOSDX010000000
VERSION     DAOSDX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9860_length_3395_cov_3.618563"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLN02_06175"
     CDS             complement(<1..472)
                     /locus_tag="PLN02_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51946.1"
                     /translation="MTNKAFWEDPYASTHSTVVTAVDGDTVELASTIFFAFSGGQESD
                     HGSIGGVAVVEASKEGRRLRYRLAAGHGLQPGQPVEVAIDWARRYRLMRLHFAAELVL
                     ELAGRALPGALKVGAHIAADKARIDFRWPESVALLLPELAASANRIIAADLPIRT"
     gene            complement(663..1247)
                     /locus_tag="PLN02_06180"
     CDS             complement(663..1247)
                     /locus_tag="PLN02_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51947.1"
                     /translation="MKIEQKKLSNHHGFTFEADRLNFAYRDKTGSGDIDVPYAEFPCK
                     SSTLIESNQWLRNVGYLWCALGVLQIVYHLYAGERPSGFWLLLGLGCLLWVRMTKVVY
                     TCYRTQYGNVLVIQDGNAHDRIIEEILDRRRRQLLAMYGEVNPENSLEQEVGRIRWLE
                     ELQVFSREEAERRIAQLELLLGTAGSASPSVTIN"
     gene            1532..2164
                     /gene="upp"
                     /locus_tag="PLN02_06185"
     CDS             1532..2164
                     /gene="upp"
                     /locus_tag="PLN02_06185"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOI51948.1"
                     /translation="MPVIEVRHPLVKHKIGLMREGEISTKKFRELAAELARLLTYEAC
                     RDFELERVSIEGWCGPVEIDQIKGKKVTVVPILRAGIGMLDGVLDLIPNAKVSVVGIA
                     RNHETLMPEPYFERFVGQLDERLAMIIDPMLATGGSLVATIDMLKRKGCKQIKALVLV
                     AAPEGIAALNAAHPEVEIYTAAVDSHLNEHGYIIPGLGDAGDKIFGTKEA"
     gene            2161..3390
                     /locus_tag="PLN02_06190"
     CDS             2161..3390
                     /locus_tag="PLN02_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HOI51949.1"
                     /translation="MNTTQDPVWRTALSGAQILFVAFGATVLVPLLTGLNPSLALLGA
                     GVGTLIFQICTKRQVPIYLGSSFAFIAPVIYSVQTWGMPATLGALASASFFYYVAAGL
                     VKWRGVGLIHKLLPPVVIGPVVMVIGLGLAQVAVGMATGKAGDAQVVPYETAIAIAAI
                     SLLATMLTAIRARGLLKLVPILVGVAVGYVVSLFLGIVDFSKVNEAAWIAMPAFGTPE
                     FNLAAILFMIPVAIAPIVEHVGGILAIGSVAGKDFTESPGLHRTLLGDGLAVNVVGLF
                     GGPPVTTYGEVTGAVMLTKNYNPVVMTWAACFAILMAFVGKFGALLQTIPLPVMGGIM
                     VLLFGSIAGIGLKTLMDARVNLSSARNLCIVSVTLVTGIGGLGVTIGSFSLQGISLCG
                     VLAVLLNQLLPTEAEQH"
BASE COUNT          604 a         1131 c         1056 g          604 t
ORIGIN      
        1 cggtgcggat cggcaggtcg gcggcaatga tccggttggc gctggccgcc agttccggca
       61 gcagcagggc aacgctctcc ggccagcgga aatcgatccg cgccttgtcg gcggcgatat
      121 gcgcgccgac cttgagcgcc ccgggcaagg cgcggccggc gagttcgagg accagttcgg
      181 cggcgaagtg gaggcgcatc aggcggtaac ggcgtgccca atcgatcgcc acctcgaccg
      241 gctggccggg ctgcaagccg tgcccggcag ccaggcgata gcggaggcgc cgcccttcct
      301 tgctcgcttc gacgacggcg acgccgccta tgctcccgtg atcgctttcc tggccgccgg
      361 aaaaggcgaa gaagatcgtt gacgccagtt cgaccgtgtc gccatcgacc gccgtcacca
      421 ccgtgctgtg cgtgctggcg taagggtctt cccagaaggc tttgtttgtc atgcgtgcgg
      481 aatatcggtt attgcccgtt agcggcgggc agtggcagtg ctccgccgcc cgattctctg
      541 ccaggcaggt aaatcggcgc aagcgccgcg catccggcga gcggatcgac tagcctcatg
      601 aggaacagac tcttattctg gggtggatgg aatccagtgg atacgatgcc cccccaaaac
      661 cctcagttga tcgtcaccga cggcgaggcg ctgccagcag taccgagcag cagttcaagc
      721 tgggcgatgc ggcgctcggc ttcttccctg gaaaaaacct gcagttcctc aagccagcgg
      781 attctgccaa cttcctgctc gagactgttt tcgggattca cctcgccgta catggcgagc
      841 agttgccgcc gccggcgatc gagtatttcc tcgatgatgc ggtcgtgcgc attgccgtcc
      901 tggatcacca agacattgcc gtattgcgtc ctgtagcagg tataaaccac cttcgtcatg
      961 cgcacccaga gcaggcaccc caagcccagc aacaaccaga aaccgctcgg tcgctccccg
     1021 gcatagaggt gatagacgat ctgcaacaca cccagcgcac accacaggta accgacgttc
     1081 ctcaaccact ggttgctttc gatgagcgtc gagctcttgc aggggaactc ggcataggga
     1141 acgtcgatat cgccggaacc cgtcttgtcg cgataggcga aattgagccg atccgcttcg
     1201 aaggtgaagc cgtggtggtt ggaaagtttt ttctgctcga ttttcatctg gacgcccccc
     1261 ggaaaaacac ttggcgcggc aagcggagcg gcaggcgggc acgcaccctt tccccagccc
     1321 ccaggcagaa tcgccgcgaa ttgaccggca ggaggatacg caaagcgacc accagcgtct
     1381 atcgcaattt cataacaaaa ccccatcgtc atcaagcacc gaagcaaggc gctccttcgc
     1441 cagcctgccc gatagccaca ccccgccgtg caagcacccg ccggatgcac taagcttcgc
     1501 actttgcgca gacgcgcgcg aggggaaaag catgccggtg atcgaagtcc gccatccttt
     1561 ggtcaaacac aagatcgggc tgatgcgcga aggcgagatc agcaccaaga aattccgcga
     1621 actggctgcc gagctggccc gcctcctgac ctacgaggcc tgccgcgatt tcgagctgga
     1681 gcgggtgagc atcgaaggct ggtgcgggcc ggtcgagatc gaccagatca agggcaagaa
     1741 ggtcaccgtg gtgcccatcc tgcgcgccgg catcggcatg ctggacgggg tgctcgacct
     1801 gatcccgaac gccaaggtca gcgtcgtcgg cattgcccgc aaccatgaga cgctgatgcc
     1861 ggagccgtat ttcgagcgct tcgtcggcca gctcgacgag cgcctggcga tgatcatcga
     1921 cccgatgctg gccaccggcg gttcgctggt cgccaccatc gacatgctga agcgcaaggg
     1981 ctgcaagcag atcaaggcgc tggtgctggt cgccgcgccg gaaggcatcg cggcgctgaa
     2041 cgcggcgcat cccgaggtgg aaatctacac cgccgccgtc gacagccacc tcaacgaaca
     2101 cggttacatc atccccggcc tgggcgacgc cggcgacaag atcttcggga ccaaggaagc
     2161 atgaacacga ctcaagatcc ggtctggcgg acggcgcttt ccggcgcgca aatcctcttc
     2221 gtcgccttcg gcgccaccgt gctggtgccg ctgctcaccg ggctgaaccc cagcctggcg
     2281 ctgctcggcg ccggcgtcgg cacgctgatc ttccagatct gcacgaaacg ccaggtgccg
     2341 atctacctcg gttccagttt cgccttcatc gcgccggtga tctattcggt gcagacctgg
     2401 ggcatgccgg cgacgctggg cgcgctggct tcggccagct tcttctatta cgtcgccgcc
     2461 ggcctggtga aatggcgcgg cgtcgggctg atccacaagc tgctgccgcc ggtggtgatc
     2521 ggcccggtgg tcatggtgat cggtctcggc ctggcccagg tcgcggtcgg catggcgacc
     2581 ggcaaggccg gcgacgcgca ggtcgttccc tacgaaacag ccattgccat cgccgccatc
     2641 tcgctgctgg cgacgatgct caccgccatc cgcgcccgcg gcctgctgaa actggtgccg
     2701 atcctggtcg gcgtcgccgt cggctacgtc gtctcgctgt tcctcggcat cgtcgatttc
     2761 tcgaaggtga acgaggcagc ctggatcgcc atgccggcct tcggcacgcc ggaattcaac
     2821 ctggcggcga tcctcttcat gatcccggtc gccatcgcgc cgatcgtcga gcacgtcggc
     2881 ggcatcctcg ccatcggctc ggttgccggc aaggatttca ccgaaagccc aggcctgcac
     2941 cgcaccctgc tcggcgacgg gctggcggtc aatgtcgtcg gcctgttcgg tggcccgccg
     3001 gtcaccacct atggcgaagt caccggcgcc gtcatgctga ccaagaacta caacccggtg
     3061 gtgatgacct gggccgcctg cttcgccatc ctgatggcct tcgtcggcaa gttcggcgcc
     3121 ctgctgcaga ccatcccgct gccggtgatg ggcggcatca tggtgctgct cttcggctcg
     3181 atcgccggca tcggcctcaa gacgctgatg gacgcccggg tcaacctgtc cagcgcgcgc
     3241 aacctgtgca tcgtctcggt gacgctggtc accggcatcg gcggtctcgg cgtgaccatc
     3301 ggcagcttct cgctgcaggg catcagcctg tgcggcgtgc tcgccgtact actcaaccag
     3361 ttgctgccga ccgaggcgga acagcactga aaagg
//
LOCUS       DAOSDX010000229         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9872_length_3392_cov_2.741085, whole genome shotgun sequence.
ACCESSION   DAOSDX010000229 DAOSDX010000000
VERSION     DAOSDX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9872_length_3392_cov_2.741085"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLN02_06195"
     CDS             complement(<1..183)
                     /locus_tag="PLN02_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51950.1"
                     /translation="MLFSIERVGDRTKNSLRPVQPCNNPHIALQATENSSLIMNPASL
                     ADLQSFAAVARLEIGKA"
     gene            182..949
                     /locus_tag="PLN02_06200"
     CDS             182..949
                     /locus_tag="PLN02_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI51951.1"
                     /translation="MQNILADLSGKTILVTGASSGIGAATALALGRAGARVALAARRL
                     AALEDLVAQIRADGGDAIAIKTDVAVEADVANAVAQTVAHFGRLDGAFNNAGVLGKVA
                     PIHELSSADFAEVMQANVYGVFWSLKYEMAAMLKTGGGAIVNTASVVAQIGFANFAAY
                     TASKHAVLGLTRSAALEGFQQGIRVNAVNPGPIVTPMAEIGFGGLENLEASLKGSPAG
                     RPGQPEEIAGPVLFLLSSAASYINGQGFAIDGGYTVQ"
     gene            962..1954
                     /locus_tag="PLN02_06205"
     CDS             962..1954
                     /locus_tag="PLN02_06205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOI51952.1"
                     /translation="MQTRFLGQQRLPVSAIGLGCMGMSEFYGASDDAESLRTLARALE
                     LGINFFDSADTYGHGHNESLLGRFLKEGGPARRQQVVIASKFGIVREPGKYERRIDNS
                     PAYIRQACEASLRRLGVEQIDLYYCHRRNPEVPIEEMMGALAQLVAAGKVRQIGLSEV
                     SAATLRHAGAVHPVAAVQSEYSLWTREAEREMLATCAELGTTFVAYSPLGRAFLTGAV
                     HSGQLAEGDFRKHNPRFVGAAEKINAQLVAELGRFAAARGLSNAQVALAWLLGKQPHV
                     VPIPGTRRIAYLEQNAGAAAVSLKPEEIAELDRMFAPERVAGARYAEAGMVGIE"
     gene            complement(2131..2481)
                     /locus_tag="PLN02_06210"
     CDS             complement(2131..2481)
                     /locus_tag="PLN02_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51953.1"
                     /translation="MKTRPPRSDPPKNPLLPLAKLFWWAGGLFAPFSIFLATHFGTPG
                     HGGPGGWGSGFFILLTGIMFISPSLVVSGVLALLAGDHLKKERYWLGGLFGLLLGGCM
                     LLLCGEIYLLLLFL"
     gene            2631..2789
                     /locus_tag="PLN02_06215"
     CDS             2631..2789
                     /locus_tag="PLN02_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51954.1"
                     /translation="MEWAVGYGIREFLADYDIGLGAFFKRVFFCGRAAWGNDAKDKFD
                     VFVVLKEY"
     gene            2852..>3392
                     /locus_tag="PLN02_06220"
     CDS             2852..>3392
                     /locus_tag="PLN02_06220"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOI51955.1"
                     /translation="MKHERPLRIGQSLAMEEAVAIDELHAQLTGEAPALVIFFCSASY
                     DLALLATEINRRFAGIPVIGCTAAGTIGPLGYGESGVSGIAFAAADFTVECGHFDKLQ
                     EFEVDAGRDRALELMLRLRERREVESGCGYFALSLIDGLSMREEQVAMALQDGLGGIH
                     VIGASAGDDLRFQRTQVFHD"
BASE COUNT          614 a         1014 c         1135 g          629 t
ORIGIN      
        1 agccttgccg atctccaggc gggcgacggc ggcaaacgac tggagatcgg caaggctggc
       61 gggattcatg attaatgagg aattctcagt agcctgtaaa gcaatgtgcg gattgttgca
      121 cggttggaca gggcgtaggc tgtttttcgt cctatcccca accctctcaa tggagaacaa
      181 catgcaaaat attttggctg acctgtccgg taaaaccatc ctcgttactg gcgcctcgtc
      241 cggcatcggc gccgccaccg ccctggcgct tggccgggcc ggggccaggg tggcgctggc
      301 cgcccgccgt ctggcggcgc tggaagacct ggtggcgcag attcgcgccg acggcggcga
      361 cgccatcgcg atcaagaccg acgtggccgt cgaagccgat gtcgcgaacg ccgtggcgca
      421 gaccgtcgcc cacttcggcc gcctcgacgg cgccttcaac aacgccggcg tgctgggcaa
      481 ggtggcgccg attcatgaac tgagcagcgc cgatttcgcc gaggtcatgc aggccaacgt
      541 ctatggtgtg ttctggtcgc tcaagtacga aatggcggcg atgctgaaga cgggcggcgg
      601 cgccatcgtc aatacggctt ccgtcgtcgc ccagatcggc ttcgccaact tcgcggccta
      661 tacggcgagc aagcacgccg tgctcggcct cacccgcagc gccgcgctcg aaggcttcca
      721 gcagggcatc cgggtcaatg cggtgaatcc cgggccgatc gtcacgccga tggcagaaat
      781 cggttttggc ggcctggaaa atctcgaagc ctcgctcaag ggcagcccgg ccggccgtcc
      841 cgggcagccg gaggaaatcg ccgggccggt gctcttcctg ctttcctcgg cggccagcta
      901 catcaacggc cagggcttcg ccatcgacgg cggctatacc gttcagtaaa ggaccatcaa
      961 catgcaaacc cgctttctcg gtcagcaacg gctgccggtc tccgccatcg gcctcggctg
     1021 catggggatg agcgaattct atggcgccag cgacgatgcc gaatccctgc gcacgctggc
     1081 ccgggcgctc gaactcggca tcaatttctt cgacagcgcc gacacctacg gtcatggcca
     1141 caatgaaagc ctgctcggcc gttttctcaa ggaaggcggc ccggcacgcc gccagcaggt
     1201 ggtgattgcc agcaagttcg gcatcgtccg cgagcccggc aagtacgagc gacgcatcga
     1261 caacagcccg gcctacatcc gccaggcctg cgaggcctcg ctgcgccgtc ttggtgtcga
     1321 gcagatcgac ctctattact gccatcgccg caacccggaa gtacccatcg aggagatgat
     1381 gggggcgctg gcgcaactgg tcgctgccgg caaggtccgc cagatcggtc tttccgaagt
     1441 gtcggcggcg accttgcggc atgccggcgc ggtgcatccg gtggccgccg tgcaaagcga
     1501 atattccctg tggacgcgcg aggccgagcg cgaaatgctc gccacctgcg ccgagctggg
     1561 cacgaccttt gtcgcctaca gtccgctcgg ccgcgccttc ctgaccggcg ccgtgcactc
     1621 cgggcaactg gccgagggcg atttccgcaa gcacaatccg cgcttcgtcg gcgcggcgga
     1681 aaagatcaac gcccaactgg ttgccgaact cggccgcttc gccgcggccc gcggcctcag
     1741 caacgcccag gtggcgctcg cctggctgct cggcaagcag ccgcatgtcg tgccgattcc
     1801 gggcacccga cgcatcgcct atcttgagca gaatgccggc gccgcggcgg tgtcgctcaa
     1861 gccggaggag atcgccgaac tcgaccggat gttcgcccct gaacgggtcg ccggggcgcg
     1921 ttatgccgag gccggcatgg tcggcatcga atagggcgtc gcgggcagct aaactttccg
     1981 tagcagttca gttggcaaag gcccttgtcg gaaaaggcga gggcctttta cataggggcg
     2041 ttcgttgacc gggacaggta tcgttacccg cgtctggttc ggctcccgat tgttgcgagt
     2101 ggcccgaggt ctttggggcc ggcgtgcggg ctagaggaac agcagcaaca agtagatctc
     2161 gccgcacaga agcagcatgc agccgcccag aagcagcccg aacaggccgc caagccagta
     2221 ccgttccttt ttcaggtggt cgccggcaag cagggccagg acgccggaga cgacaaggct
     2281 cgggctgatg aacatgatac cggtcagcag gatgaagaag ccgctgcccc agccgccggg
     2341 gccgccgtgg ccaggggtgc cgaagtgggt tgcgaggaaa atgctgaacg gcgcgaacag
     2401 tcctcctgcc caccaaaaaa gtttggccag gggcaggagc ggatttttcg gtgggtcgga
     2461 gcggggaggg cgggtcttca cgggacgctc tgggacaatg cccttcttga atgccgccag
     2521 ttcgtgtccg gtggcgcagg ccggaaaatc gcaggcggcg caggattcgg tgccggcagc
     2581 gttgtgctgt tggcagttct gacaagtcca gcgataggat ggcggggaca ttggaatggg
     2641 ctgtcggtta cggcatacgg gaatttctgg cggattatga catcggcctt ggtgcgtttt
     2701 tcaagagagt gtttttttgt ggaagagcgg cgtggggaaa tgatgcaaaa gataaatttg
     2761 acgtttttgt tgttttgaag gaatattgat ggcagaaatt ttctgcttcg cgtcgaattg
     2821 aggcgcgaca gaaagctatc aaggacaagc catgaagcat gaacgacccc tacgtatcgg
     2881 acagtcgctg gcgatggagg aagccgtcgc cattgacgaa cttcacgcgc aactgaccgg
     2941 ggaagccccg gcgctggtga ttttcttctg ttcggcgagt tatgacctcg ccctgctggc
     3001 gaccgaaatc aatcgccgct ttgccgggat tccggtgatc ggctgtaccg cggcggggac
     3061 catcgggccg ctcgggtatg gcgagagcgg cgtctcggga atcgcctttg cggcggccga
     3121 tttcaccgtc gagtgcgggc atttcgacaa gctgcaggag ttcgaggttg acgccgggcg
     3181 tgatcgggcg cttgagttga tgctgcgtct gcgcgagcgt cgcgaagtcg agtccggttg
     3241 cggttatttc gcgttgtcgc tgatcgacgg cctgtcgatg cgtgaggaac aggtggcgat
     3301 ggcgctgcag gacggcctcg gcgggattca tgtgattggc gcctcggcag gcgacgatct
     3361 gcgtttccag cgcacgcagg tgtttcatga tg
//
LOCUS       DAOSDX010000230         3389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9880_length_3389_cov_2.970306, whole genome shotgun sequence.
ACCESSION   DAOSDX010000230 DAOSDX010000000
VERSION     DAOSDX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9880_length_3389_cov_2.970306"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLN02_06225"
     CDS             complement(<1..202)
                     /locus_tag="PLN02_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOI51956.1"
                     /translation="MLVDSHCHLDFPGLSERLPEVLATMREAGVGAAMTIGVNLEDLP
                     AVLAVAEQADHLYASVGVHPEYT"
     gene            complement(209..571)
                     /locus_tag="PLN02_06230"
     CDS             complement(209..571)
                     /locus_tag="PLN02_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOI51957.1"
                     /translation="MSEVKPVPQRPSVLSLNINSKSALYAAFMPHLRNGGIFIPTTRV
                     YALGDEVFMLLSLMDDPTKLPIAGSVIWVTPAGAQNGRAQGIGVHFNDDESGQEARRK
                     IEGLLGGVLQSARPTHTL"
     gene            complement(578..1603)
                     /gene="holB"
                     /locus_tag="PLN02_06235"
     CDS             complement(578..1603)
                     /gene="holB"
                     /locus_tag="PLN02_06235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOI51958.1"
                     /translation="MFIKVFDLHKEIWSSLLARRDRLPHALLLIGQKGLGKYALAREF
                     AAALLCESGAPDGRACGGCLACNWFAQGNHPDFRLLQPEALSEEVAAEEGKKKPSQQI
                     TIDQVRELDEFLGVGSHRGGLSIVLVNPAEAMNRNAANSILKTLEEPPPNTLFLLVSS
                     DPSRLLPTIRSRCQSIPFVTPSRETATRILGAAGLRDAERWLAISGGSPPLALELAES
                     GQVAWLDRLATNLAAGGKADALACAAELDKAIKDSKGGLQLKQIVEALQKWTVDLTLA
                     GNGLPIRYFLAQQSQISALAAMIPPVRLIHQYRDMNQRRREAEQPLNARLFLESVFLD
                     YRAMFSR"
     gene            complement(1594..2208)
                     /gene="tmk"
                     /locus_tag="PLN02_06240"
     CDS             complement(1594..2208)
                     /gene="tmk"
                     /locus_tag="PLN02_06240"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOI51959.1"
                     /translation="MRGKFITFEGIDGAGKSSHVEWLAEYLRRQGRVVQVTREPGGTP
                     LGERLRELLLNETMHLETETLLMFAARREHLASRIEPALARGEWVICDRFSDATWAYQ
                     GGGRGLDKSKFLALEHWVHEHLQPDLTLLFDLPLEVARERIVLANRVLDKFEQERADF
                     HERVRQAYLERAHAHPARMRVIQADNTLENIREKLEEIVSTLCL"
     gene            complement(2205..3173)
                     /gene="mltG"
                     /locus_tag="PLN02_06245"
     CDS             complement(2205..3173)
                     /gene="mltG"
                     /locus_tag="PLN02_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOI51960.1"
                     /translation="MFLLAAAAIVAAFVWWANRPLDLPGEAVEFRVAPGSSLHGTIQQ
                     INAAGIPLQADLFKLLARWQGKVNAIKAGSYATDSGETPYTLLVKLVEGRVSQASVTL
                     VEGWTFRQFRARLDAHPALRHDSRTLDEAAISARLGLPWQQLEGRLLPDTYLFDKESS
                     DLEVIARAAAAMQKQLGAAWAGRPAGLPYNSPDEALIMASIIEKETGRPEDRPHVAAV
                     FINRLRQGMRLQTDPTVIYGLGENFDGNLRKSHLQTDTPYNTYTRAGLPPTPIAMPGP
                     ASLRAAFQPAASDALYFVARGDGSSHFSRTLDEHNRAVNRYQRGGK"
     gene            3057..>3389
                     /locus_tag="PLN02_06250"
     CDS             3057..>3389
                     /locus_tag="PLN02_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51961.1"
                     /translation="MQAATGRHPEFDGLARQIKRPIRPPDEGGNNRSGGEQKQGDKAK
                     HETHGNSRAEIGSCRSETARQGASGGWQNGRYNSPNLKEPPMNPNWRSFLESAEANFA
                     ADATDVLDF"
BASE COUNT          637 a         1049 c         1060 g          643 t
ORIGIN      
        1 cggtgtattc gggatgtacg ccgaccgagg cataaaggtg gtcggcttgt tcggcgacag
       61 ccagcacggc aggcaggtct tcgagattga cgccgatcgt cattgccgcg ccaactccgg
      121 cctcgcgcat tgtcgccagc acttcgggaa gacgttcgct cagccccgga aaatccaggt
      181 ggcaatggga atcgaccagc atcgcgtgtt acagggtatg cgtcgggcga gccgattgca
      241 ggacgccgcc gagcaggcct tcgatcttgc gccgtgcctc ttggccgctt tcatcatcat
      301 tgaaatgcac gccgatacct tgcgcccgac cgttctgggc accggccggc gtcacccaga
      361 ttaccgagcc ggcgatcggc agcttggtgg gatcatccat cagggaaagc agcatgaaga
      421 cttcatcgcc caacgcatag acgcgcgtcg tcggaatgaa gatgccgcca ttgcgcagat
      481 gcggcatgaa tgcggcataa agagcggact ttgaattgat gttcagcgac aggacgctgg
      541 gacgttgcgg tacgggtttg acttcgctca tcctggatca acggctaaac atggctcgat
      601 agtccaaaaa gacactttcc agaaacagtc gtgcgttgag aggctgttcc gcctcgcggc
      661 gacgctggtt catgtcccgg tactgatgga tcagacgcac aggcggaatc atagcagcga
      721 gcgctgatat ttgggactgc tgtgccagaa aatatctgat tggcaagccg ttcccggcca
      781 gcgtcagatc gactgtccat ttctgcaggg cctcgacaat ctgcttgagt tgcaagccgc
      841 ccttgctgtc cttgatcgcc ttgtcgagtt cggctgcaca agccagcgca tcggccttgc
      901 caccagcagc gagattcgtc gccagtcggt cgagccaggc gacctgcccg gattccgcca
      961 gttccagggc cagcggcggc gaaccgccgc tgatcgccag ccagcgttcg gcgtcgcgca
     1021 ggccggcggc accgagaatg cgtgtcgcgg tttcgcgtga cggcgtgacg aaaggtatcg
     1081 actgacaacg gctgcggatc gtcggcaaaa gccgggatgg atcacttgaa accaataaaa
     1141 acaaggtatt tggcggtggt tcttcaagtg ttttcaagat cgagttcgcg gcattccggt
     1201 tcatcgcttc cgccggattg accaggacaa tactcaatcc tccacggtga ctgccaacgc
     1261 cgagaaactc gtcgagctcg cgcacttggt cgatggtgat ctgctggctc ggcttcttct
     1321 ttccctcctc tgccgccact tcctcgctca acgcctccgg ttgcaacaag cggaagtcgg
     1381 ggtggttgcc ctgggcgaac cagttgcacg ccaggcagcc accgcatgcc cgaccgtccg
     1441 gcgcgccgct ctcgcacaac agcgcggcgg caaattcgcg ggccagagcg tacttgccta
     1501 ggcctttctg accgatcagc aacaaggcgt gcggcaacct atcccgccgc gccaaaaggc
     1561 tggaccaaat ctctttgtgt agatcaaaaa cttttataaa catagtgttg aaacgatttc
     1621 ttcaagtttt tcacgaatat tttccagcgt attgtcggct tgaatcactc gcatccgagc
     1681 gggatgggca tgtgcccgct cgagataggc ctgacgcacc cgttcgtgga agtccgccct
     1741 ttcctgttcg aatttgtcga gcacccggtt ggctagaacg atgcgctcgc gcgccacttc
     1801 cagcggcagg tcgaagagca gcgtcaggtc gggttgcagg tgctcatgga cccagtgctc
     1861 cagcgccagg aacttgctct tgtcgagccc gcgaccgccg ccctgatagg cccaggtggc
     1921 gtcgctgaaa cggtcgcaga tcacccattc gccgcgcgcc agcgccggtt cgatacggct
     1981 agccaggtgt tcgcggcggg cggcgaacat cagcagggtt tccgtctcca gatgcatggt
     2041 ctcgttgagc agcaattccc gcagccgctc gccgagcggg gtgccaccgg gctcacgcgt
     2101 cacctggacg acgcgcccct ggcgacgcag gtactcggcc agccactcga cgtgactgct
     2161 cttgccggca ccgtcgatgc cttcgaaagt gatgaacttg cccctcattt gccgcctcgc
     2221 tgataacggt tgaccgcccg gttgtgttcg tcgagcgtcc gcgaaaaatg gctgctgccg
     2281 tcgccgcgcg cgacgaaata caaggcatcg ctggccgccg gctggaacgc ggcacgcagc
     2341 gaggccgggc ctggcatcgc gatcggcgtc ggcggcaggc cggcgcgggt gtaggtattg
     2401 tacggggtgt cggtctgcag atggctcttg cgcaggttgc cgtcgaaatt ctcgcccagg
     2461 ccgtagatca ccgtcggatc ggtctgcagg cgcatgcctt ggcgcaaacg gttgatgaag
     2521 acggcggcga catgggggcg gtcttccggg cgcccggttt ccttttcgat gatcgacgcc
     2581 atgatcagcg cctcatccgg cgagttgtaa ggcaggccgg cgggccgccc ggcccaggct
     2641 gcaccgagtt gcttctgcat cgcggcggca gcccgggcga tcacctcgag atcgctggat
     2701 tccttgtcga acaggtaagt atccggcagc agccggcctt cgagctgctg ccagggcagg
     2761 ccgaggcggg cgctgatcgc ggcttcgtcg agcgtgcggc tgtcgtggcg cagcgccgga
     2821 tgggcgtcaa gccgtgcccg gaactggcgg aaagtccaac cttcgaccag cgtgacgctg
     2881 gcctggctga cccggccctc aaccagtttg acgaggagcg tgtacggtgt ctcgccgctg
     2941 tcggtagcat aactgccggc cttgatcgca ttgaccttgc cctgccagcg ggccagcagt
     3001 ttgaatagat cggcctgcag cgggatgccg gccgcgttga tctgctggat cgttccgtgc
     3061 aggctgctac cgggcgccac ccggaattcg acggcctcgc caggcagatc aagcggccga
     3121 ttcgcccacc agacgaaggc ggcaacaatc gcagcggcgg cgagcagaaa cagggcgaca
     3181 aggcgaaaca cgaaacgcat gggaactcgc gggcggaaat cgggtcttgc cggtcggaaa
     3241 ccgcacggca aggcgcatcg ggcggctggc aaaacgggcg atataatagc ccaaacctca
     3301 aggagccccc catgaatccc aactggcgca gctttctcga atcggccgaa gcgaacttcg
     3361 ccgccgacgc gaccgacgtt ctcgacttt
//
LOCUS       DAOSDX010000231         3389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9883_length_3389_cov_2.542292, whole genome shotgun sequence.
ACCESSION   DAOSDX010000231 DAOSDX010000000
VERSION     DAOSDX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9883_length_3389_cov_2.542292"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLN02_06255"
     CDS             <1..259
                     /locus_tag="PLN02_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51962.1"
                     /translation="AALAQARSVRRVEPMEGVVNERERSLQQDMAEVDPRAVGPYQGV
                     ERRRQQADQLARRLVDMGSDAMQLLRFDTRARRPGKIDEKA"
     gene            343..2331
                     /locus_tag="PLN02_06260"
     CDS             343..2331
                     /locus_tag="PLN02_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOI51963.1"
                     /translation="MSGLNPQQREAIYYLDGPLLVLAGAGSGKTRVITQKIAYLVQDC
                     GFQPRNIAAITFTNKAAREMQERVGKLLSASQANELQISTFHSLGVRILREEAKALGY
                     KPRFSIFDSADCAGIIGDCAKTVDKATLRRLQSIISNWKNALVTPEAARHLAANEHEA
                     LAASAYLSYEATLKAYQAVDFDDLIGLPVKLFSEHPQIAEKWQNRLRYLLVDEYQDTN
                     ACQYQVLKLLTGIRAQFTAVGDDDQAIYGWRGADIDNLKKLPAEFPALKVIKLEQNYR
                     STVRILKAANNVIAHNEKLFDKTLWSELGHGDPIHVTACRDNEAEAESVVMKLQAHRF
                     EHRGKFRDYAILYRSNHQARLLETQLRNQKIPYLLSGGQSFFDKAEIKDIISYLRLLA
                     NEDDDPAFIRAATTPRRGIGAATLQALGTYAGERHVSLFHAAFEEGFAHRAQARQLEP
                     LLEFCHFINRMQARATREPAQQVLPDLLKAIDYEAYLYDSEEQRTAQTRWANVGEFAD
                     WINRKGEEDGKTLIDMVQSIALINMLDKQNSDEIDAVQLSTLHAAKGLEYKHVFLVGV
                     EEGILPHRESLDPAKLQEERRLMYVGITRAQRSLHISYCEKRKQARELIPCEPSRFID
                     EMGKDDIRFSGGKQAVAPDKATGSARLDAMKALLAGKR"
     gene            complement(2401..2856)
                     /locus_tag="PLN02_06265"
     CDS             complement(2401..2856)
                     /locus_tag="PLN02_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOI51964.1"
                     /translation="MAETYSSKRIMWAMIVVAVVLIAIIYPLTNKSFNSAADAGASAG
                     ADEAELRIQPVAGFALAKADAAAATGAPKDGPTVYNSVCGACHNTGAAGAPKIDDKAA
                     WAPRVATGNDALLASVLNGKNAMPPKGGASALSEDEIKGALDYILSKVK"
     gene            3112..>3389
                     /locus_tag="PLN02_06270"
     CDS             3112..>3389
                     /locus_tag="PLN02_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOI51965.1"
                     /translation="MNELELLAGRLGAALSERGEWLACAESCTGGWVAQSVTAIAGSS
                     GWFERGFVTYSNAAKIEMLGVAEATLARHGAVSEAVARAMAQGALAHSR"
BASE COUNT          655 a         1054 c         1084 g          596 t
ORIGIN      
        1 ggcggcgctc gcccaggcca ggagcgtgcg tcgggtggaa ccgatggaag gcgtggtcaa
       61 tgagcgcgaa cgctcgttgc aacaggacat ggccgaggtc gatccgcgtg cggtcggccc
      121 ttatcagggc gtcgaacgac ggcgtcagca ggcggaccag cttgcccgac gtctggtcga
      181 tatgggttcg gatgccatgc agttgctgcg cttcgacact cgcgcccggc gcccgggcaa
      241 gattgacgag aaggcctgac ggcggcgatt ctggcatggg gtaaaatgcg cggccccggc
      301 gacaaccctg tcgccgcgag ttatacaggt tcctgcgtag acatgtccgg cctcaatccc
      361 cagcaacgcg aagcgatcta ctaccttgac ggtcccctgc tcgtcctggc cggcgccggc
      421 tcgggcaaga cccgggtgat cacgcagaag atcgcctatc tggttcagga ttgcggcttc
      481 cagccgcgca acatcgccgc catcaccttc accaacaagg cggcgaggga aatgcaggag
      541 cgcgttggca agttgctctc cgcgtcgcag gccaacgagt tgcagatttc gacctttcac
      601 tcgctcggcg tgcgcatcct gcgcgaggaa gcgaaggcgc ttggctacaa gccgcgtttc
      661 tcgatcttcg attcggccga ttgcgccggc atcatcggtg attgcgcgaa gaccgtggac
      721 aaggcgacct tgcgtcgtct gcagtcgatc atctcgaact ggaagaacgc cctggtcacg
      781 cccgaggccg cccgccacct ggcggccaac gaacacgagg cgctcgccgc cagcgcctat
      841 ctcagctacg aagcgacgct gaaagcctac caggcggtcg atttcgacga cctgatcggc
      901 ttgccggtga aactgttcag cgaacatccg caaatcgccg aaaagtggca gaaccgtctg
      961 cgctacctgc tggtcgacga ataccaggat accaacgcct gccagtacca ggtgctcaag
     1021 ctgctgaccg ggatccgcgc ccagttcacg gcggtgggcg acgacgatca ggcgatctac
     1081 ggctggcgcg gcgccgacat cgacaacctg aagaagctgc cggcggaatt cccggcgctc
     1141 aaggtgatca agctcgagca gaattatcgg tcgacggtgc gcatcctgaa ggccgccaac
     1201 aacgtcatcg cccacaacga gaagctgttc gacaagacct tgtggtcgga actggggcat
     1261 ggcgacccga tccacgtcac tgcctgccgc gacaacgagg ccgaggccga gagcgtggtc
     1321 atgaagctgc aggcgcaccg cttcgagcat cgcggcaagt tccgtgacta cgccatcctg
     1381 taccgctcca accatcaggc gcgcctgctc gaaacccagt tgcgcaacca gaagattccc
     1441 tatctgctgt ctggcggcca gtccttcttc gacaaggccg agatcaagga catcatcagc
     1501 tatctgcgcc tgctcgccaa cgaggacgac gatccagcct tcattcgtgc cgcgaccacg
     1561 ccgcgccgcg ggattggcgc cgctaccctg caggcgctcg gcacctatgc cggcgagcgt
     1621 cacgtttcgc tcttccatgc cgctttcgag gagggctttg cccatcgcgc gcaggctcgc
     1681 cagctcgaac ccctgctcga gttctgccat ttcatcaacc gcatgcaggc tcgggcaacg
     1741 cgcgagccgg cgcagcaggt gctgcccgat ctgctcaagg cgatcgatta cgaggcttac
     1801 ctctacgaca gcgaggagca gcgcacggcg cagacgcgct gggccaatgt cggcgaattc
     1861 gccgactgga tcaatcgcaa gggtgaggaa gatggcaaga ccttgatcga catggtgcag
     1921 agcatcgccc tgatcaacat gctcgacaag cagaacagcg acgagatcga cgccgtccag
     1981 ctatcgacgc tgcatgcggc gaaagggctg gagtacaagc acgtcttcct ggtcggcgtc
     2041 gaggaaggca tcctgcctca tcgcgaatcc ctcgatccgg ccaagctgca ggaagagcgg
     2101 cgcctgatgt acgtcggcat cacacgcgcc cagcgctctt tgcacatcag ctattgcgag
     2161 aagcgcaagc aggcgcgtga actgattccc tgcgagccgt cgcgtttcat cgacgagatg
     2221 ggcaaggacg atatccgctt cagcggtggc aagcaggcgg tggcgccgga caaggccacc
     2281 ggcagtgcgc gactcgatgc gatgaaggca ttgctggcag gcaagcgctg agcgcgctgc
     2341 ggacatcccg gtcaacgcaa aagggaggcc gcagcctccc tttgtcgatc cgggacggga
     2401 ttacttgacc ttgctcagga tgtagtcaag cgcacccttg atttcgtctt cggagagtgc
     2461 cgaagcgcca cccttgggcg gcatggcatt cttgccattg agtaccgaag cgagcagggc
     2521 gtcgttgccg gtggcgacgc gcggcgccca ggcagccttg tcgtcgatct tcggcgcacc
     2581 agccgcaccg gtgttgtggc aggcgccaca gacgctgttg taaaccgtcg ggccatcctt
     2641 cggcgcgccg gtggccgcgg cggcgtcagc cttggccagc gcgaaaccgg caaccggctg
     2701 gatgcgcagt tcggcttcgt cagccccggc actcgcgcca gcgtcggcgg cggaattgaa
     2761 ggacttgttg gtcaaaggat agatgattgc gatcaaaacg acggcgacga cgatcatcgc
     2821 ccacatgatg cgcttggaag aatatgtttc ggccatgctc aagaccccgt ttgtatagtg
     2881 gactaaatga tgaattatag cgattcgcgt atcattgagc caaaaaaaac ccccgccggc
     2941 ggcgggggga gcaaatgtct cgaatggggg aattcgagag gaggggttca atgcccttgc
     3001 agtttatgac ggcggcagcg tctcgtctgt cactctttcg tgcccgttgt gtaaccaaat
     3061 gtggcgcaac ggccggtgtt tccgccggta cgttgggctg cgggatttgc catgaacgaa
     3121 cttgaattgc tcgccggccg actcggcgcc gccctgagcg agcgcggcga atggctggcc
     3181 tgcgccgaat cctgtaccgg tggctgggtc gcccagtcgg tgacggcgat tgccggcagt
     3241 tccggctggt tcgagcgcgg tttcgtcacc tattccaacg ccgccaagat cgagatgctg
     3301 ggcgtcgctg aagcgacgct ggcgcgtcat ggcgcggtgt cggaggcggt cgcgcgggcg
     3361 atggcgcagg gggcgctggc gcacagtcg
//
LOCUS       DAOSDX010000232         3388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9886_length_3388_cov_3.752175, whole genome shotgun sequence.
ACCESSION   DAOSDX010000232 DAOSDX010000000
VERSION     DAOSDX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9886_length_3388_cov_3.752175"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLN02_06275"
     CDS             complement(<1..378)
                     /locus_tag="PLN02_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOI51966.1"
                     /translation="MTYFAGIDIGSTAIKTVLINAGGEIVGIHVTDSGSLFHKNAKEA
                     LRVLLEKSGVDRAQLKYLIATGYGRKLFKEADDSISEITANAIGAHETGKAYGGVRTI
                     INIGGQDSKAIQIDDSGNVSNFVM"
     gene            complement(375..1658)
                     /locus_tag="PLN02_06280"
     CDS             complement(375..1658)
                     /locus_tag="PLN02_06280"
                     /inference="COORDINATES: protein motif:HMM:NF040772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HOI51967.1"
                     /translation="MSEIREVPLKPAFNQSRQAREAEVMLNHIVDDFIDNPAAMTYFY
                     DLFRHAFIEGKALDRDGPGIGTTCIQVPEELIYAAGGTPVRLCNGAYHYDQVGADFMP
                     AKSCSLVKATLGRLHSEKAVPDVGKLELIVNPTTCDQKKKAAQMMEGMGYQVYDLDLP
                     SAKDNEAAREYWRRVVKQLAARLQKVTGKKLTRQRLREAMAKTSRAQAAFRTLHNFRR
                     KAPSLILGKDVFLVTNAYFFDDIERWSAAVEALNAELAQREAEAFSAVQKKAPRIVFT
                     GSPPIFPNIKLPLLIEEAGGVVVADETCSANRMLYDMTAVDEWLVSHMIESIADKYLK
                     PCTCPIFTRNDDRIRRLLDLVSTFNADGVVYQSFAGCQVYEMEQRSVAEALNKAGIPM
                     LYIETDYSPDDMGQLSTRVEAFLESIKTRKRQARA"
     gene            complement(1655..2626)
                     /locus_tag="PLN02_06285"
     CDS             complement(1655..2626)
                     /locus_tag="PLN02_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI51968.1"
                     /translation="MSKLRDAVNNVDLKRFRFWFQLAAFVLFVYGGYLAINLGNSLPM
                     FSCGYIREGRAGVCYLLPLQHQLARPWGQLFGYAGVAVLTGFAIFALWFIVLNKGWCG
                     FVCPLGTLQDWLTSLRQKLGIRQSGYTHEQFRRLTWIKYVLLALLLLIPLGIGGGLWS
                     HEVGAPFCQICPGRMVLPLFVGDFSQWTIDFSSPGKIVLTALGMGITGLFIVGAFVKK
                     RFFCFFCPMSALHYLLSKPALLRLRKDGSKCTRCGDCYTACDMEIREIADDVTRKEIM
                     TDDCTLCLKCVAACPEKGALKVDFANITFFESTEEGFIKRMNKGIRK"
     gene            complement(2619..>3388)
                     /locus_tag="PLN02_06290"
     CDS             complement(2619..>3388)
                     /locus_tag="PLN02_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51969.1"
                     /translation="NPQTAGSVPASEPVLLLDAQQQASAIDVEPHGGPYNVTFPMPEP
                     GVYNAYFVRRAVDRETLAVTVAKAEVMRSAGHAKVEGEDRLMVPRTDARVPVEIVRER
                     KDGEGLFTRINYGDTIRFQVLRDGKPVQNARVSFTSGQGWSNSVQSDEDGRAGFVVIR
                     DYFPEWREFDRRHRETFVVSASFPLDQAGQWQGTPYRDVRYTTTLAGAYYPAIEDYES
                     YAWGLGIAVVALLFSGTAIYLYRRRRVRPYREVSFDE"
BASE COUNT          570 a         1038 c         1148 g          632 t
ORIGIN      
        1 catcacgaag ttgctgacgt tgccgctgtc gtcgatctgg atcgccttcg aatcctggcc
       61 gccgatgttg atgatggtgc gcacgccgcc gtaggccttg ccggtctcgt gggcgccgat
      121 ggcgttggcg gtgatttccg aaatcgaatc gtcggcttcc ttgaacagct tgcggccgta
      181 gccggtggcg atcaggtatt tcagttgcgc ccggtccacg ccggatttct ccagcagcac
      241 gcgcagcgcc tccttggcgt tcttgtggaa gaggctgccg gagtcggtga cgtggatgcc
      301 gacgatctcg ccgcccgcgt tgatcagtac ggtcttgatg gcagtggagc cgatgtcgat
      361 gcctgcgaag taagtcatgc gcgagcctgt cttttacggg tcttgatgga ttcgaggaag
      421 gcctcgacgc gggtcgatag ctggcccatg tcgtccgggc tgtagtcggt ttcgatgtag
      481 agcatcggga tgccggcctt gttcagcgct tcggcaacgc tgcgctgctc catctcgtac
      541 acctggcagc cggcgaagga ctggtagaca acgccgtcgg cattgaaggt ggagacgagg
      601 tcgagcaggc gccggatgcg gtcgtcgttg cgggtgaaga tcgggcaggt gcagggcttc
      661 aggtacttgt cggcgatgct ctcgatcatg tggctgacca gccattcgtc gaccgccgtc
      721 atgtcgtaga gcatgcggtt ggccgagcag gtctcgtcgg cgacgacgac gccgccggct
      781 tcctcgatca gcagcggcag cttgatgttg gggaagatcg gcggcgaacc ggtgaacacg
      841 atgcgcggcg ccttcttctg cacggcgctg aaggcttcgg cctcgcgctg cgccagttcg
      901 gcgttgagcg cctcgacggc ggcgctccag cgctcgatgt cgtcgaagaa ataggcgttg
      961 gtcacaagga agacatcctt gccgaggatc agcgacggcg ccttgcggcg gaagttgtgc
     1021 agggtgcgga aggcggcctg ggcgcgcgag gtcttggcca tcgcctcgcg caggcgctgc
     1081 cgggtcagct tcttgccggt cactttctgc aggcgtgccg ccagttgctt gaccacccgc
     1141 cgccagtatt cgcgcgccgc ttcgttgtcc ttggcgctcg gcaggtccag gtcgtagacc
     1201 tggtagccca tgccttccat catctgggcg gctttcttct tctggtcgca ggtcgtcgga
     1261 ttgacgatca gctccagctt gccgacgtcg ggcacagcct tttccgaatg caggcggccg
     1321 agcgttgcct tgaccagcga gcaggacttg gccggcatga agtcggcgcc gacctgatcg
     1381 taatggtagg caccgttgca caggcggacc ggcgtgccgc cggcggcgta gatcagctct
     1441 tccggcacct ggatgcaggt ggtgccgatg cccggcccgt cacggtcgag cgctttgccc
     1501 tcgatgaagg cgtggcggaa gaggtcgtag aagtaagtca tcgccgccgg gttgtcgatg
     1561 aagtcatcga cgatgtggtt gagcatgacc tcggcttcgc gcgcctggcg gctctggttg
     1621 aacgccggct tgagcggcac ttcgcggatt tcactcactt gcggattccc ttgttcatgc
     1681 gcttgatgaa accttcctcg gtcgattcga agaaggtgat gttggcgaaa tcgaccttca
     1741 gcgcgccctt ttccgggcag gcggcgacgc atttgaggca cagcgtgcag tcgtcggtca
     1801 tgatttcctt gcgcgtcacg tcgtcggcga tctcgcggat ttccatgtcg caggcggtgt
     1861 agcagtcgcc gcagcgggtg catttgctgc catccttgcg caggcgcagc agcgccggct
     1921 tggagagcag gtaatgcagt gcgctcatcg ggcagaaaaa gcagaagaag cgcttcttga
     1981 cgaaggcgcc gacgatgaac aggccggtga tgcccatgcc cagcgcggtg aggacgatct
     2041 tgccggggct ggagaaatcg atcgtccatt gcgagaagtc gccgacaaac agcggcagca
     2101 ccatccgccc cgggcagatc tggcagaacg gcgcgccgac ctcgtgcgac cacaggccgc
     2161 cgccgatgcc aagcgggatc agcagcagca aggcgagcag cacgtacttg atccaggtca
     2221 gccggcggaa ctgttcgtgc gtatagcccg actggcggat gcccagcttc tggcgcaggc
     2281 tggtcagcca gtcctgcaag gtgccgagcg ggcagacgaa gccgcaccag cccttgttga
     2341 ggacgatgaa ccacagcgca aaaatggcga agccggtgag cacggcgacc ccggcgtaac
     2401 cgaacaattg cccccagggc cgcgccaact ggtgctgcag cggcagcagg taacagacgc
     2461 cggcccgccc ctcgcggatg tagccgcagc tgaacatcgg caggctgtta ccgaggttga
     2521 tcgccaggta gccaccataa acgaagagga cgaaggcggc gagctggaac cagaagcgga
     2581 agcgcttcag gtcgacgttg ttgaccgcgt cgcgcaactt actcatcgaa gctcacctcg
     2641 cggtagggcc gcacccggcg ccgccggtag aggtagatcg ccgtacccga gaagagcagc
     2701 gcgacgacgg cgatgcccag cccccaggcg taggactcgt aatcctcgat cgccggatag
     2761 taggcaccgg ccagcgtcgt cgtgtagcgc acgtcgcggt agggcgtgcc ctgccattgg
     2821 ccggcctgat cgagcgggaa gctggcgctg accacgaagg tctcgcggtg gcgacggtcg
     2881 aactcgcgcc attccgggaa atagtcgcgg atgacgacga agccggcgcg gccgtcctcg
     2941 tcggactgca cgctgttgct ccagccctgg ccgctggtga agctgacgcg ggcgttctgc
     3001 accggcttgc cgtcgcgcag cacctggaag cggatggtgt cgccgtagtt gatacgggtg
     3061 aacagccctt ccccatcctt gcgctcgcgg acgatctcga ctggtacccg ggcgtcggtg
     3121 cgcggcacca tcaggcggtc ttcgccttcg accttcgcat gcccggccga acgcatcacc
     3181 tcggccttgg cgacggtcac cgcgagcgtt tcacggtcaa cggcccggcg cacgaaatag
     3241 gcgttgtaga cgcccggttc gggcatcggg aaagtcacgt tgtaagggcc gccgtgcggc
     3301 tccacatcga tcgccgatgc ctgctgctgc gcatcgagca gcaacaccgg ctcgctcgcc
     3361 ggcacgctgc ccgccgtctg cgggttgc
//
LOCUS       DAOSDX010000233         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9911_length_3382_cov_2.361587, whole genome shotgun sequence.
ACCESSION   DAOSDX010000233 DAOSDX010000000
VERSION     DAOSDX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9911_length_3382_cov_2.361587"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3382
                     /locus_tag="PLN02_06295"
     CDS             <1..>3382
                     /locus_tag="PLN02_06295"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51970.1"
                     /translation="LSSKTASLTQNLSAAEAAHGTTLTLGSNVTGINSVQVQKYINGA
                     WVTLIDSAVDTNANRLEIAGLPAAAVSVQLEYRPKGDSGPYVAKPEAVVSKLGTGWFA
                     LSYDDIANGEYEYRLTVKDSAGNPLSLTALGGNAQGQVSGTFSVLRGGQALPSVTAGS
                     VSIVTPLTRFENDVFGNVIHQASYIKGAAQASADGYTIAGQDAANDRHAYNVYNLHGH
                     LVQSVDAEGASVYFSYDAYGRVAKRWQALTDSSGATRNAVTLYSYDASGLQRETSEVV
                     DTKTLATSEVKYNAFGEIVAKGLNGVYSEFVDYDNAGRVLRSNSDKGVTTAFLSDLQG
                     LATASIRSATIDLSAAAYKSPSALAALSQNVVRTNTVYDALGRPVKQALPSLTVDQSP
                     DAIIAPLVVGAQYLDIGALESVVSEVKTGSEGGYEGSTTVKRAELSWSDLGVWGSGAT
                     RVIVNYTYQYLFNSTKTAALTQELTAQEAAHGVSVTLGATTTAINSVQLQKFINGTWV
                     TVVDSAADMVRDSILVWERPAVEGTQVVLRYRAQGSSGAWSTATINTLGERCWVDLSA
                     LPADRYEYVLSYTRPGESKAYAEGSGVIAVARGASTNSSNIVGEFVTPYLDIGPIETV
                     VSSITTSTEGGYQSAASTQKVAFAWSDLGAWGNGNARVVVNYTYKYFLTTKSATLTQN
                     LSAAEAAHGTLLTFATGISAVSSVQVQKFINGAWVTVIDGTSDVVKPAQLVLRGKTAG
                     LSAVEVRDANNALVATCSLANGKLKALAGDVFVADITDVQAGNYSYKPVGTTSLAGGS
                     FVKEGGAVSGRVQIGEKALTLDIGPLESVVTGITTGSEGGYAGATSAQKLELSWDDLG
                     AWGSGAARVVVGYKNSAGSTASLTQNLSEAEAAHGTTLTFANQVFLYSVIKVTSITSV
                     QVQKYIDGAWVTLINSSADTNSSRLEITALPAAAASVSLEYRAKGEDGPYVSKPASAF
                     SKLGTGWFGLSYDDIANGEYEYRLTVKDSAGQLLNLTALGGTAQGLIAGAFTVLRGGQ
                     SIVGAPAPQLVKLDPVVNRTVDRWGNVLSLSDPAAAGRITNFRYNHANQLISEIKPET
                     DIWNESGVNQRSDGVANDRPTTRYAYDARGGLIATTDAN"
BASE COUNT          707 a          988 c         1084 g          603 t
ORIGIN      
        1 ctttccagca aaaccgcttc gctgactcaa aacctgtcgg cagcggaagc tgcgcacggc
       61 actacgttga ccttgggcag caatgtgacc gggatcaata gcgtccaggt tcaaaagtac
      121 atcaatggtg cctgggtaac gctgatcgac agcgcggttg acacgaatgc caatcgcctc
      181 gagatcgcag ggttgccggc agcagcggtc agcgtgcaac tggaataccg gccaaagggc
      241 gacagcgggc catacgttgc caagccggag gccgttgtca gcaaactcgg gacgggatgg
      301 ttcgcccttt cctacgatga catcgccaac ggcgaatacg aataccggct gacggtgaaa
      361 gatagtgccg gcaatccgct cagtctgacc gccctgggcg gtaacgccca aggtcaggtc
      421 tccgggactt tcagcgtcct gcgcggtggc caggccctgc cttcggttac cgccggtagc
      481 gtcagcatcg tcacgccgtt gacccgcttc gaaaacgacg tttttggcaa tgtcatacac
      541 caggcttcct atatcaaggg ggccgcccag gccagcgctg acggttacac gattgccgga
      601 caggatgccg cgaacgaccg gcatgcctac aacgtataca atctgcatgg gcacctcgtg
      661 caatcggtgg acgctgaggg cgcctcggtg tacttctcct acgatgccta tggtcgggtg
      721 gccaagcgct ggcaggcgct gaccgacagc agcggtgcga cacggaacgc cgttaccctc
      781 tactcctatg acgcttccgg cctgcagcgg gagacgtccg aggttgtcga cacgaagacg
      841 ctggcgacga gcgaagtgaa gtacaacgcg ttcggggaaa tcgtcgccaa gggcctgaac
      901 ggcgtgtact cggaattcgt cgactacgac aatgccgggc gggtgctgcg cagcaacagc
      961 gacaaaggcg tgaccacggc atttctttcc gatttacagg ggttggcaac tgcaagcatc
     1021 cggagcgcga ccatcgatct ttcggcggct gcctacaaga gtccgtccgc gcttgccgcc
     1081 ctgagccaga acgttgtgcg taccaatacg gtttatgacg cactggggcg tccggtcaag
     1141 caagccttgc cgtcattgac cgtcgatcag agtcccgatg ccatcattgc tccgctggtg
     1201 gttggcgccc aatacctgga tatcggtgcc ctcgaaagcg tggtgagcga ggtcaagacg
     1261 gggagtgaag gcgggtacga aggatcgacg acagtcaagc gcgcggagtt gtcgtggagc
     1321 gatcttggcg tctggggatc gggcgcgacc cgggtcatcg tcaattacac ttatcaatat
     1381 ctttttaact cgaccaagac ggctgcgctg acccaggaac tgactgcgca ggaagcagca
     1441 cacggggtaa gcgtgacgct cggcgccacc actaccgcca tcaacagcgt ccagttgcag
     1501 aagttcatca acggtacctg ggtcaccgta gtcgacagcg ctgcggatat ggtgcgcgac
     1561 agcatcctgg tctgggagcg gccggcggtt gagggaacgc aagtggtttt gcgttatcgc
     1621 gcacaaggca gctctggagc ctggagcacg gcgacgatca acacgctggg cgaacgctgc
     1681 tgggtcgatc tgagcgcctt gccggcggat cgctacgagt atgtgctgag ctatacccgg
     1741 ccaggcgaga gcaaagccta tgccgaagga agcggcgtga tcgcggtggc tcgcggggcg
     1801 agcacgaaca gcagcaatat tgtcggcgaa ttcgtcacgc cctatctgga catcggcccg
     1861 atcgagacgg tggtgagcag tattacgaca agtacggaag ggggctatca gagtgcggct
     1921 tctacccaga aagtcgcgtt tgcctggagc gatctcggag cctggggcaa tggcaatgcc
     1981 cgggtcgtgg tgaactacac ctacaagtat ttcctgacca caaaatcggc gaccctgacg
     2041 caaaacctga gtgccgcgga ggcggcgcac gggacgctgc tgacgttcgc taccggtatc
     2101 tctgcggtca gcagcgtcca ggtccagaag ttcatcaacg gggcctgggt taccgtgatc
     2161 gatggcacga gtgatgtcgt caaaccggcg caactggtat tgcgcggcaa gacagctggc
     2221 ttgagcgcgg tggaggtgcg cgatgcgaat aacgcattgg tggcaacctg cagtctggcg
     2281 aacggcaagc tgaaagcgct tgctggggat gtttttgtcg ccgatatcac cgacgtgcaa
     2341 gccgggaatt acagttacaa gccggtaggc acgacatccc ttgcgggggg gagtttcgtc
     2401 aaggagggcg gtgccgtctc cggccgggtg cagatcggcg agaaggcgct caccctggac
     2461 atcggaccgc tcgaaagcgt ggtgaccggg atcacgacgg gtagcgaggg cggctatgcc
     2521 ggtgccacat cggcgcaaaa gctggaactc tcgtgggacg atctgggcgc ctggggctca
     2581 ggcgccgccc gggttgtggt tggctacaag aatagcgccg gtagcacggc ttcactcacg
     2641 caaaacctga gtgaagccga agcggcgcat gggactacgc tgacatttgc gaatcaggtc
     2701 ttcctatatt ccgtcattaa agtgacgagc atcaccagcg tccaggtaca aaagtacatc
     2761 gacggcgcct gggtcacgct gatcaacagt tcggccgaca ccaacagctc ccgcctggaa
     2821 atcaccgctt tgccggccgc ggcggcgagc gtctcgctgg aataccgggc gaagggcgag
     2881 gatggtcctt acgtgagcaa gccggcatcc gcgttcagca agttgggcac gggatggttc
     2941 ggtctctcct acgatgacat cgccaacggc gaatacgaat accggctgac ggtcaaggac
     3001 agtgccgggc agttgctcaa tctgactgcg ctgggcggca ctgctcaggg gctgattgcc
     3061 ggtgccttca cggttctgcg cggcggccaa agcatcgtcg gcgcgccggc gccgcaactg
     3121 gtcaagctgg accccgtcgt caatcgcacc gtcgatcgct ggggcaatgt cctctcgctg
     3181 tccgatccgg cagcggccgg gcgcatcacc aatttccgct acaaccatgc caaccagctg
     3241 atctccgaaa tcaagccgga gaccgatatc tggaacgaaa gtggcgtcaa ccagcgcagc
     3301 gacggcgttg ccaacgaccg gccgacgacg cgctacgcct acgacgcgcg cggcggcttg
     3361 atcgccacca ccgacgccaa cg
//
LOCUS       DAOSDX010000234         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_9985_length_3360_cov_3.029955, whole genome shotgun sequence.
ACCESSION   DAOSDX010000234 DAOSDX010000000
VERSION     DAOSDX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9985_length_3360_cov_3.029955"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLN02_06300"
     CDS             complement(<1..1038)
                     /locus_tag="PLN02_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOI51971.1"
                     /translation="MSVSAEISSLIEPILQRHAWRADRLVQILREVQEAVDFISDETA
                     AHLAEALKIPYAQVHSTRDFYAFFYRENRGRYRVLFSDNITDRMAGNQALFEHMAKRL
                     KLEPGKVSDDGLVSIDYCSCTGMCDQGPAILVNNLAITRMTLRRVDEICDLIRGQVPL
                     DKWPAEFFRVDDNIRRADMLLGTPHEPGKAVRAAIARGRDGMLAEMKLSNLRGRGGAG
                     FTTAVKWEACRNAPATPRYIVCNADEGEPGTFKDRVLLQSYAGRVFEGMVIAAYTVGA
                     SHGFLYLRGEYLYLLQPLEAYLAGMRAAGLLGRDIGGVAGFDFDIEIHLGAGAYVCGE
                     ETALIESLEGKA"
     gene            1442..2230
                     /gene="fabI"
                     /locus_tag="PLN02_06305"
     CDS             1442..2230
                     /gene="fabI"
                     /locus_tag="PLN02_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HOI51972.1"
                     /translation="MSSSNSTTALPLAGKKGIVTGIANDKSIAYAVARAIVELGGEIA
                     VTYQNEKTAKYTQPLAEALGAKLFLKLDVAEPGCLEGVIAQCGEAFGEIDFAIHSMAF
                     CNADDLHGRVIDASEAGFDSAMNVSCHSFLRMAKALEPLMAHGGSLITMSYLGAERVV
                     RNYGVMGIIKAALESAVRYMAYDLGPKGIRVFAVSPGPIMTRAASGIANFNNLLESDA
                     QKAPLGRTVTIEEVGALTAFLCTPGSSGMTGQTIYVDAGAHIVA"
     gene            2304..2882
                     /locus_tag="PLN02_06310"
     CDS             2304..2882
                     /locus_tag="PLN02_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51973.1"
                     /translation="MSEPTTPIIPDPVPDHPLLKGRKEIGRGESTIVVEGDTVDGQER
                     VFKILSSPTDYAYYTAPDRPTGRHFPIVYADHGTAGKSSRGFPFHVVEVERLYPLPGS
                     GETAEVATKISTAYFDACMMWRNLAQDMGRIALHHLTATPMGWADTVRESLQALETFS
                     GEYGALPDLIKADNLMMRKDGTLVFSDPVFME"
     gene            2914..>3360
                     /locus_tag="PLN02_06315"
     CDS             2914..>3360
                     /locus_tag="PLN02_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51974.1"
                     /translation="MTINRVFYTSRKLAESLSGNPYMAVISITDPTTPQARLDPLFRH
                     VLRLSFFDAVPADEFMPPLPGLFDRTMARRVDEFVREIHGAPFEMSMMVHCEYGVSRS
                     AAVALFVEAATGAPLEAREFTYEANLWVVDSLQALHPGMEIDIPSPL"
BASE COUNT          626 a         1144 c         1021 g          569 t
ORIGIN      
        1 ggccttgcct tcgagcgatt cgatcaacgc agtttcctcg ccgcagacgt aagccccggc
       61 accgagatgg atttcgatgt cgaaatcgaa accggcgacg ccgccgatgt cgcggccgag
      121 cagaccggcg gcgcgcatgc cggcgaggta ggcttcgagc ggctgcagca ggtagaggta
      181 ttcgccgcgc aggtagagga agccgtggct ggcgccgacc gtgtaggcgg cgatgaccat
      241 gccctcgaac acccggccgg cgtaggactg gagcaggacg cggtccttga aagtgcccgg
      301 ctcgccctcg tcggcgttgc agacgatgta gcgcggcgtg gccggggcgt tgcggcaggc
      361 ttcccacttg acggcggtgg tgaagccggc gccgccgcgg ccgcgcaggt tggacagctt
      421 catttcggcg agcatgccgt cgcggccgcg ggcgatcgcc gcgcgcaccg ccttgcccgg
      481 ctcgtgcggc gtgccgagca acatgtcggc gcggcggatg ttgtcgtcga cgcggaagaa
      541 ttcggccggc cacttgtcga gcggcacctg gccgcggatc aggtcgcaga tttcgtcgac
      601 ccggcgcagc gtcatccggg tgatcgccag gttattgacc aggatcgccg gcccctggtc
      661 gcacatgccg gtacaggagc aatagtcgat gctcaccagc ccgtcgtccg agaccttgcc
      721 cggttcgagt ttgaggcgct tcgccatgtg ctcgaacaag gcctggttgc cggccatgcg
      781 gtcggtgatg ttgtccgaga agaggacccg gtagcggccc cggttctcgc ggtagaagaa
      841 ggcgtagaag tcccgcgtgc tgtgcacctg ggcatacgga atcttcaagg cctcggccag
      901 atgggccgcg gtttcatcgg aaatgaagtc cacggcttcc tggacctcgc gcaggatctg
      961 cacaagacgg tccgctcgcc aggcgtggcg ctgcaggatc ggctcgatca gactggaaat
     1021 ctcggctgag acagacacgg taggcatccc ctggaaaaca gaccacaaca ctctagagct
     1081 ttgtcccggc gcgaatttgc gctggatcaa ataaacccga ggtcaacagc gaaaagtagc
     1141 tgccggaaat tcgtaaaaca ctgataagga aagtgttttt tagccaatac agaaaatgac
     1201 aaaaggattt tttatcgcag cgcaacaagc cgcctcggca gaagtaagcc agcgtttttc
     1261 gacgctgcag gagatttgcc gggcgatgcc gtgccacatg acgaaaccgc cacccatttg
     1321 atgcaacaaa gtagcggatg gacgtcacaa ttgcccgcgg caaaacgtgc taatctgcgc
     1381 cgcatggtgc agcgcagcat gcgcgctgcc gctttcacga caaccctccg cgagaaaacc
     1441 catgagttcc agcaattcca ccactgccct gccgctggcc ggcaagaagg gcatcgtcac
     1501 cggcatcgcc aacgacaagt cgatcgccta cgcggtcgcc cgcgccatcg tcgaactcgg
     1561 tggcgaaatc gccgtcacct accagaacga gaagaccgcc aaatacaccc agccgctggc
     1621 cgaagccctc ggcgccaagc tcttcctcaa gctcgacgtc gccgagccgg gctgcctcga
     1681 aggcgtcatc gcccagtgcg gcgaagcctt cggcgaaatc gacttcgcca tccactcgat
     1741 ggccttctgc aacgccgacg acctgcacgg ccgcgtcatc gatgcgtccg aagccggctt
     1801 cgattcggcg atgaacgttt cctgccacag cttcctgcgc atggccaagg cgctggagcc
     1861 gctgatggcg cacggcggtt cgctgatcac catgtcctac ctcggtgccg aacgcgtcgt
     1921 gcgcaactac ggcgtcatgg gcatcatcaa ggcagcgctc gaatcggccg tccgctacat
     1981 ggcttacgac ctcggtccca agggcatccg cgtcttcgcc gtgtcgcccg gcccgatcat
     2041 gacgcgcgcc gcttccggca tcgccaactt caacaacctg ctcgaatccg acgcccagaa
     2101 ggcgccgctc ggccgcaccg tgaccatcga ggaagttggc gccctgaccg ccttcctgtg
     2161 cacgccgggt tcgtcgggca tgaccgggca gaccatctac gtggacgccg gcgcccacat
     2221 cgtcgcctga cccccgcagc ggcgcgggac gaccggtgtc gtcctgcgct atgctgacgc
     2281 ctgccccaag cggcggatac gacatgagcg aaccgacaac cccgatcatt cccgaccccg
     2341 ttcccgacca ccccctgctc aaggggagga aggaaatcgg tcgcggcgaa agcaccatcg
     2401 tcgtcgaagg cgataccgtc gacggccagg aacgggtctt caagattctt tcctcgccga
     2461 ccgactacgc ctattacacg gcgcccgacc ggccgaccgg ccgccatttc ccgatcgtct
     2521 acgccgatca tggcaccgcc ggcaagtcga gccgcggctt ccccttccac gtcgtcgagg
     2581 tcgaaaggct ctatccgctg cccggcagcg gcgagacggc cgaggtggcg accaagatct
     2641 cgaccgccta tttcgacgcc tgcatgatgt ggcgcaacct ggcccaggac atgggccgca
     2701 tcgccctgca ccacctgacg gcgacgccga tgggctgggc cgatacggtg cgcgagtcgc
     2761 tgcaggcgct ggagaccttt tccggcgaat acggcgccct gcccgacctg atcaaggccg
     2821 acaacctgat gatgcgcaag gacggcacgc tggtcttctc cgaccccgtc ttcatggaat
     2881 gacctttcgc ttatttcccg gaacgggccc acgatgacca tcaaccgcgt tttctacacc
     2941 tcgcgcaagc tcgccgaatc gctttccggc aacccctaca tggcggtgat ctcgatcacc
     3001 gacccgacga cgccgcaagc ccggctcgac ccgctgttcc gccacgtgct gcgcctctcc
     3061 ttcttcgacg cggtgccggc cgacgaattc atgccgccgc tgcccggcct cttcgaccgg
     3121 accatggcgc gccgcgtcga cgagttcgtg cgcgaaatcc acggcgcacc gttcgagatg
     3181 tcgatgatgg tccattgcga atatggcgtc agccgctcgg ccgccgtcgc gctgttcgtc
     3241 gaggctgcca ccggcgcccc gctggaagcc cgcgaattca cctacgaagc caatctctgg
     3301 gtggtcgaca gcctgcaggc cttgcacccg gggatggaaa tcgacattcc gtcgccgctg
//
LOCUS       DAOSDX010000235         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10001_length_3357_cov_2.989703, whole genome shotgun sequence.
ACCESSION   DAOSDX010000235 DAOSDX010000000
VERSION     DAOSDX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10001_length_3357_cov_2.989703"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLN02_06320"
     CDS             complement(<1..275)
                     /locus_tag="PLN02_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51975.1"
                     /translation="MSYTLHHPSTARRSGLLTVVIGLHASILLAVLAAKTIAPQIMEM
                     PLVVDLLPAPQIEAEPQAKPLPVAKPEPVRQKPLPTPKTPAPVLETT"
     gene            complement(376..552)
                     /locus_tag="PLN02_06325"
     CDS             complement(376..552)
                     /locus_tag="PLN02_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HOI51976.1"
                     /translation="MNSPPKTTPPANPVPNNSNILRLESGEILRGANTVEIEHAGQRY
                     LLRVTRENKLILTK"
     gene            complement(636..1889)
                     /locus_tag="PLN02_06330"
     CDS             complement(636..1889)
                     /locus_tag="PLN02_06330"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HOI51977.1"
                     /translation="MSTFPYHVVGGSFSASPMAIKPVPAKDISAPASTRRRKLWELSH
                     KCHCPVIGTCFEVAELRGLLKKVLDFPRDTSDFVLHTTAVGACESRSTVAELLHKQLE
                     KRYPLTVRKFSAARDNAALYALWRAEVAAGNGIPAALWATLSHPACDATLEHEVYADI
                     HMIQHQIGSGNRADLRTLKQQRDEISELRRQLEKARQENEALRAERSAETQSLAQRIV
                     ELRAELAGSAARFAQLTGQYEELRNSLPELRERQSLLRRAADAEARNQALAAEAASQE
                     TELRRLREQLQTAEEMIAQLSRHDEAPEEGGMRPEQTGERLSGKCVLCVGGRAGMVEA
                     YRHLVEQSGGRFIHHDGGLEESLHRIDGALTAADLVICQAGCISHNAYWRVKEQCKRT
                     GKRCVFVRNSGVSSFGRGLDEAMEN"
     gene            complement(2040..2411)
                     /locus_tag="PLN02_06335"
     CDS             complement(2040..2411)
                     /locus_tag="PLN02_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51978.1"
                     /translation="MQLRELFVIGQPGPPPACTAAAGENVTLLRHLGRIQQRCTRLIT
                     ELQQRIADLEAGNRRLHAELRRCEEENAALRQQLGHHEASELLCRTGCLPLDQQWRDE
                     RDQCRLDGRPCPHAKDEISAS"
     gene            complement(2413..>3357)
                     /locus_tag="PLN02_06340"
     CDS             complement(2413..>3357)
                     /locus_tag="PLN02_06340"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOI51979.1"
                     /translation="HVAAVDGIVRQLLPTLSRLRETLAGKARDFADIVKIGRTHLQDA
                     TPLTLGQEISGWVAQLEHAERHIRANLPHLHELAIGGTAVGTGLNAPPGYAQRVAAEL
                     ARHTGHPFVSAPSKFEALASVDAIVHAHGSLKGLAASLMKIANDVRWLASGPRCGIGE
                     LRIPENEPGSSIMPGKVNPTQSEALAMLAAQVFGNDVAINFGGASGNFQLNVFRPLII
                     HNLLQSIRLLADGMDSFDRHCAAGIVPDRQRIGEHLERSLMLVTALNPHIGYDKAAEI
                     AKKAHREGSTLRAAALALGYVCGEDFDRWVVPGNMTGR"
BASE COUNT          486 a          947 c         1202 g          722 t
ORIGIN      
        1 cttgtggttt cgaggacggg agccggtgtt tttggcgttg gtaacggttt ttgccggacc
       61 ggctcgggtt tggccaccgg cagcggtttg gcttgtggtt cggcttcgat ctgcggtgcc
      121 ggcagcaaat cgacgaccaa gggcatttcc atgatctgcg gggcgatggt ttttgctgcc
      181 aggactgcta gcaggatgct ggcatgcaga ccaatgacga cagtcagtaa accgctgcgc
      241 cgggccgtcg agggatgatg aagtgtgtag ctcatggcga tgttcaggaa ggtcccgcgc
      301 tcgcccaaaa aaacccttgg cttgctggca tctggcgcgc tggctgtggg aacggttttt
      361 acaacggaga gaagcttatt tggtcaggat cagcttgttt tcacgggtga cgcgcagcag
      421 gtagcgctgg cccgcgtgtt cgatttcgac ggtattcgct ccacgcagga tttccccgct
      481 ttcgagccgg agaatgttgc tgttgttcgg cacggggttc gctggcggtg tggtcttggg
      541 tgggctgttc attttggtgt tgcgcgttcc gaaggcgatg agcggatttt atcgaaatgg
      601 gaacgattgt caataacatt cgtttctgcg ttttctcagt tttccatcgc ctcgtcgagt
      661 ccccggccga aactgctgac gccgctgttg cgcacgaaca cgcagcgttt gccggtgcgc
      721 ttgcattgtt ccttgacccg ccagtaagcg ttgtgactga tgcagccagc ctggcagatg
      781 acgaggtcgg cggcggtcag ggcgccatcg atgcggtgca ggctttcttc cagcccgccg
      841 tcgtggtgga tgaagcggcc gccggactgt tcgaccagat gacggtaggc ctccaccatg
      901 ccggcacggc cgccgacgca gagcacgcat ttgccggaca ggcgttcgcc ggtctgctcg
      961 ggacgcattc ctccttcttc aggtgcttcg tcgtgtcggc tcaactgggc aatcatttct
     1021 tcggcggtct gcagttgttc ccgcaggcgc cgaagttcgg tctcctggct tgcggcttct
     1081 gccgccagcg cctggttgcg cgcttcggca tcggcggcgc gccgcagcag ggattgcctt
     1141 tcccgaagtt ccggcaggct gttgcgtaat tcctcgtact ggccggtcaa ttgggcgaaa
     1201 cgggcggcgc tgccggccag ttcggcgcgc agctcgacga tgcgctgggc gagcgattgc
     1261 gtttcggccg accgctcggc gcgcaatgct tcgttctctt ggcgggcttt ttccagttgg
     1321 cggcgcaatt cgctgatttc gtcacgctgc tgtttcagcg ttctcaggtc ggcccggtta
     1381 ccgctgccta tctggtgctg gatcatgtgg atgtcggcgt agacctcatg ttccagcgtc
     1441 gcatcgcagg ccggatggct cagtgttgcc cacagggcgg ccgggatgcc gttgccggcg
     1501 gcgacttcgg cacgccacag cgcgtacagc gcggcgttgt cgcgggctgc cgagaatttc
     1561 cggacggtca gcggatagcg tttttccagc tgtttgtgca gcagttcggc aaccgtcgag
     1621 cgtgattcgc aggcgccgac cgctgtcgtg tgcagcacga aatcgctggt gtcgcgggga
     1681 aagtccagga ccttcttcaa caggccgcgc aattcggcga cttcgaaaca ggtgccgatt
     1741 accggacagt ggcatttgtg cgacagctcc cacagcttcc ggcggcgagt cgatgccggt
     1801 gcggaaatat cctttgccgg caccggtttg atggccatag ggctggcgct gaaactgccg
     1861 ccgacgacgt gataagggaa ggtgctcatg ttctgcctcc gggggggtcc gcagcgcttc
     1921 gagacttgcg gggtttctgg attgatgaag ggaagcactg tgttgttgcg tagcgcggcg
     1981 tccggatcag cgggctgacg ctactgcgca ggtgatatcg gccagaatgg tgagcgacgt
     2041 tatgaggcgc tgatttcatc tttggcgtgc gggcaaggcc ggccgtcgag acggcactga
     2101 tcgcgctcgt cgcgccactg ctgatcgagg ggcaggcagc cggtgcggca gagcagttcg
     2161 ctggcttcat ggtggccgag ctgctgacgc agggcggcgt tttcctcctc gcagcggcgc
     2221 agttcggcgt gcagtcgccg gttgccggct tcgaggtcgg cgatgcgttg ctgtagttcc
     2281 gtgatcagcc gcgtgcagcg ttgctggatc cggccgaggt ggcgcagcag tgtgacattt
     2341 tcgccagcgg ccgcggtgca ggccggtggc gggccaggct gaccgatgac gaagagttcg
     2401 cgcagttgca tgtcagcggc cggtcatgtt gccgggaaca acccagcggt cgaaatcttc
     2461 gccgcacacg tagccgaggg cgagtgctgc ggcgcgcagg gtgctgcctt cgcgatgtgc
     2521 cttcttggcg atttccgccg ccttgtcgta gccgatgtgc gggttgagcg cggtgaccag
     2581 catcagcgag cgttcgaggt gttcgccgat gcgttgccgg tcggggacga ttccggcggc
     2641 gcaatggcgg tcgaaactgt ccatgccgtc ggcgagcagg cggatgctct gcagcaggtt
     2701 gtggatgatc agtggccgga agacgttgag ctggaagttg ccggaggcgc cgccgaagtt
     2761 gatggcgacg tcgttgccga aaacctgggc ggcgagcatg gccagcgcct cgctctgggt
     2821 cggattcacc ttgccgggca tgatcgatga gccgggttcg ttttccggga tgcgcagttc
     2881 gccgatgccg cagcgcgggc cgctggccag ccagcgcacg tcgttggcga tcttcatcag
     2941 gctggcggcc agccccttga ggctgccgtg ggcgtggaca atggcgtcga cgctggccag
     3001 cgcctcgaac ttgctcggcg cactgacgaa ggggtggccg gtatggcgag ccagttcggc
     3061 ggcgacccgc tgggcgtagc cgggcggcgc gttgaggccg gtgccgacgg cggtgccgcc
     3121 gatcgccagt tcgtgcaggt gcggcaggtt ggcgcggatg tgccgctcgg cgtgttcgag
     3181 ttgggcgacc cagccggaaa tttcctggcc cagcgtcagc ggcgtcgcat cttgcaggtg
     3241 ggtgcggccg atcttgacga tgtcggcgaa gtcgcgcgcc ttgccggcca gggtttcgcg
     3301 caggcgggaa agggtgggca gcagttggcg aacgatgccg tcgacggcgg cgacgtg
//
LOCUS       DAOSDX010000236         3344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10057_length_3344_cov_2.492551, whole genome shotgun sequence.
ACCESSION   DAOSDX010000236 DAOSDX010000000
VERSION     DAOSDX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10057_length_3344_cov_2.492551"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..993
                     /locus_tag="PLN02_06345"
     CDS             37..993
                     /locus_tag="PLN02_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI51980.1"
                     /translation="MVKDEIKTVLNLQPLLQERVIGQDHALAAIVQRVRTARANLEDP
                     NKPKGVFLFVGPSGVGKTETALALADILYGGERKLVTINMSEYQEAHSVSGLKGSPPG
                     YVGYGEGGVLTEAVRRNPYSVVLLDEVEKAHPDVLELFFQVFDKGVLDDAEGREIDFR
                     NTLIILTSNVGSSQIMQACLNKPAEELPAADALAEALRPVLFKSFKPAFLGRLKVVPY
                     YPISDAVLLQIIELKLRRIQARIAANHKAEFSWDDKLVEAVLARCTEVDSGARNVDHI
                     LNGTLLPEIAEAVLTRMAEGTAIGRIRVSAAKNGNFKYSLAS"
     gene            990..>3344
                     /gene="tssI"
                     /locus_tag="PLN02_06350"
     CDS             990..>3344
                     /gene="tssI"
                     /locus_tag="PLN02_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015438073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HOI51981.1"
                     /translation="MNRLLDLIGGAAGWRQTDRLLTLTTPAGPDALLAETAHIDEALG
                     PVAGHAGFRIDLTVLTADAHQSLTALQGQPARLELQTALSRTEHRPFHGHISGVERLG
                     ANGGFARYRLRIEPWLAFLDHNTDNYLFQDKSVIEIVDELLGDWHGRGKLVPAWRWDL
                     ADAAVYPKRGMTVQYQESDLAFLKRLLIEEGLFCWFEHAQEEGETLGKHTLVIADHNG
                     AFTDNAQPRIRYTQPGATLPEDSVDRWLARERIDTAESLSQSWDYRSLAGRPQSALSE
                     RAAGALPATAAWHDPGQYAWQSQADGERMLGNLRQALDARLARQSAEGSVRTAMPGSF
                     FTLADHPAGDGRYLITAVSHRARNNLNERVPQLSELLGPAPDETPDETPDHFAKPADL
                     YRNRLETVAADQPWRALIADAHGRHIAPRPTGRGMLTGVVVGPGDPTHTDRDLRVRVQ
                     FPWQRGSQAGGRHSHPTGADNAPASDALGVWLRVATPVAGANWGGHLTPRPGQEVVVA
                     FQNGNIDRPVIVGTVYNGQGQGDAQGNRIAGSTLNASANAPTFFAGEQAEPHSHGASL
                     SGLKTQQLANTRDGQGGYNQLVFDDTPGQARIELSTTEYQSRLQLGHLREQDDNARLN
                     HRGHGADLATQASAALRAGSGLLISADARNNASSTHLDSREPIAQTENALSLSRSLAE
                     VAAKQNAALKGDPAAEQLPALEALKHSIEVLETTDTRGPAQAADGPIKTTRGGDGTVP
                     AWSEPRLHYAAPAGIAQLTPNNHFLFAGKNLSIASGQDSNLIAQG"
BASE COUNT          613 a         1294 c         1043 g          394 t
ORIGIN      
        1 cccccgcggc atccctccca atccgtgcgg ggaaagatgg tcaaggacga aatcaagacc
       61 gtgctcaacc tgcagcccct gctgcaggag cgggtgatcg gccaggacca tgcgctggcg
      121 gcgatcgtgc agcgggtacg cacggcgcgg gcgaacctgg aggatccgaa caagcccaag
      181 ggcgtcttcc tgttcgtcgg gccgtccggc gtcggcaaga ccgaaaccgc gctggcgctg
      241 gccgacatcc tgtatggcgg cgagcggaag ctggtcacca tcaacatgag cgagtaccag
      301 gaagcgcaca gcgtctccgg cctcaagggc tcgccgccgg gttatgtcgg ctacggcgag
      361 ggtggggtgc tgaccgaggc ggtgcgccgc aacccgtaca gcgtcgtgct gctcgacgaa
      421 gtcgagaagg cgcacccgga tgtgctcgaa ctcttcttcc aggtcttcga caagggcgtg
      481 ctcgacgacg ccgaagggcg cgagatcgat ttccgcaaca cgctgatcat cctgaccagc
      541 aacgtcggct ccagccagat catgcaggcc tgcctgaaca agccggcgga agaactgccg
      601 gctgccgacg cgctggccga agcgctgcgc ccggtgctct tcaagtcgtt caagccggcc
      661 ttcctcggcc gcctgaaggt ggtgccgtac tacccgattt ccgacgcggt gctgctgcag
      721 atcatcgaac tcaagctgcg ccgcatccag gcgcgcatcg ccgccaacca caaggccgag
      781 ttcagctggg acgacaagct ggttgaagcg gtgctggcgc gctgtacgga agtcgattcc
      841 ggcgcccgca acgtcgatca catcctcaac ggcacgctgc tgccggagat tgccgaagcg
      901 gtgctgacgc gcatggcgga aggcacggcg atcggtcgta tccgtgtcag cgcggcgaag
      961 aacggcaact tcaagtacag cctggcgtca tgaaccggct cctcgacctg atcggcggcg
     1021 cggcgggctg gcggcaaacc gaccgcctgc tgacgctgac caccccggcc ggccccgacg
     1081 ccctgctggc ggagacggcg cacatcgacg aagcgctcgg gccggtggcc ggccacgccg
     1141 gctttcgcat cgacctcacc gtgctcaccg ccgacgccca ccagtcgctg accgcgctgc
     1201 aggggcagcc ggcgcggctg gaactgcaga ccgcgctgtc gcgtaccgaa caccggcctt
     1261 tccacggcca catcagcggc gtcgaacggc tcggcgccaa cggcggtttc gctcgctacc
     1321 ggctgcgcat cgaaccctgg ctcgccttcc tcgaccacaa caccgacaac tacctgttcc
     1381 aggacaagag cgtcatcgag atcgtcgatg aactccttgg cgactggcac ggccgcggca
     1441 agctggtgcc ggcctggcgc tgggacctgg ccgatgcggc cgtctacccg aagcgcggga
     1501 tgaccgtgca gtaccaggaa agcgatctcg ctttcctcaa acgcctgctg atcgaagaag
     1561 gtctgttctg ctggttcgag cacgcccagg aagagggcga gacgctcggc aagcacacgc
     1621 tggtcatcgc cgaccacaac ggcgccttca ccgacaacgc ccagccgcgc atccgctaca
     1681 cccagcccgg cgccaccttg ccggaagaca gcgtggaccg ttggctggcg cgcgagcgca
     1741 tcgacaccgc ggaaagcctg agccagagct gggactaccg cagcctcgcc ggccgtccgc
     1801 aaagcgcctt gagcgagcgc gccgccggcg cgctgccagc caccgccgcc tggcacgacc
     1861 ccggccagta cgcctggcaa agccaggccg acggcgagcg catgctcggc aatctgcgcc
     1921 aggcgctcga cgcccggctc gcccggcaga gcgccgaagg cagcgtgcgc acggccatgc
     1981 ccggcagctt cttcaccctc gccgatcatc cggccggcga cggccgctac ctgatcaccg
     2041 ccgtcagcca ccgcgcccgc aacaacctca acgagcgggt gccgcaactc agcgaactgc
     2101 tcggcccggc gccggacgaa accccggacg aaaccccgga ccacttcgcc aagccggccg
     2161 acctctaccg caaccgcctc gaaaccgtcg ccgccgacca gccctggcgg gcgctgatcg
     2221 ccgacgccca cggccgccac atcgccccgc ggccgaccgg gcgcggcatg ctgaccggcg
     2281 tcgtcgtcgg ccccggcgac cccacgcaca ccgaccgcga cctgcgcgtg cgcgtccagt
     2341 ttccctggca gcgcggcagc caggccggcg gccggcacag ccacccgacc ggcgccgaca
     2401 acgccccggc cagcgacgcg ctcggcgtct ggctgcgcgt cgccaccccg gtcgccggcg
     2461 ccaactgggg cggccacctg acgccgcgtc ccggccagga agtcgtcgtc gccttccaga
     2521 acggcaacat cgaccgcccg gtcatcgtcg gcaccgtcta caacggccag ggacagggcg
     2581 acgcccaggg caaccggatc gccggcagca cgctcaacgc cagcgccaac gcgccgacct
     2641 tcttcgccgg cgaacaagcc gaaccgcaca gccacggcgc cagcctgtcc gggctcaaga
     2701 cccagcaact ggccaacacg cgcgacgggc agggcggcta caaccagctc gtcttcgacg
     2761 acaccccggg ccaggcgcgc atcgaactat ccaccaccga ataccagagc cgcctgcaac
     2821 tcggtcacct gcgcgaacag gacgacaacg cccggctcaa ccaccgcggc cacggcgccg
     2881 acctcgccac ccaggcgagc gccgcgctgc gcgccggcag cggcctgctg atcagcgccg
     2941 acgcccggaa caacgccagc agcacccacc tcgacagccg cgaaccgatc gcccagacgg
     3001 aaaacgcgct gagcctctcg cgcagcctgg ccgaggtcgc cgccaaacag aacgccgccc
     3061 tcaaaggcga ccccgccgcc gagcaactcc cggccctcga ggccctcaag cacagcatcg
     3121 aagtcctcga aaccaccgac acccgcggcc ccgcccaagc cgccgacggc ccgatcaaga
     3181 ccacgcgcgg cggcgacggc accgtcccgg cctggagcga accgcgactg cactacgccg
     3241 ccccggccgg catcgcccag ctgacgccga acaaccactt cctgttcgct ggcaagaacc
     3301 tgtcgatcgc cagcggccag gacagcaacc tgatcgccca gggc
//
LOCUS       DAOSDX010000237         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10170_length_3314_cov_2.923596, whole genome shotgun sequence.
ACCESSION   DAOSDX010000237 DAOSDX010000000
VERSION     DAOSDX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10170_length_3314_cov_2.923596"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLN02_06355"
     CDS             complement(<1..457)
                     /locus_tag="PLN02_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOI51982.1"
                     /translation="MQHTKKLATLAAACLFPILAQAQSNVTIFGLLDVNLAQERANGL
                     KRNALDTSELNGSRLGFKGSEDMGNGLKAIFMLESAVAIDTGTSTGFTRQSYLGLEGS
                     FGRVTFGRQYSPAFVAIDPYEATGSSDRSAGMLHRKSGSVARGYQVRFDN"
     gene            716..1483
                     /locus_tag="PLN02_06360"
     CDS             716..1483
                     /locus_tag="PLN02_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HOI51983.1"
                     /translation="MNPMTGLAAIFLAALLTVRPALAGQEDVDPLGSARWGDMRYLFP
                     EQATLRFDSRVRVLAPEVAENPMQVPVTVDASELGPVSEVMVCADFNPIVEVLRFYPQ
                     AAPAWLGFRIKLQQSSPVRAAARTADGVWHVGGAWVTTVGGGCTAPSAKAAAPDWQAR
                     LNQVSGRQWESGAQAGRVRLRIEHPMDTGLVAGIPAFHLEQVELLADGRSLMRLQLHE
                     PVSENPVFTLKRGAAEGPVEVRGHDNNGNAFQARIAP"
     gene            1480..2418
                     /locus_tag="PLN02_06365"
     CDS             1480..2418
                     /locus_tag="PLN02_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     1"
                     /protein_id="HOI51984.1"
                     /translation="MKRWLPLAFLGVALAAAGADFDYRLSAERVADDVHVFVGKTEDF
                     TPANGGNIVNTGFIVAPGGIVVIDSGPSRRYGEQMRRAIAAVDGRPLALVINTHHHPD
                     HFLGNQAYADVPLAALPGTIEGIASDGNAFAENLYRLTGDWMAGTEVVVPKQALAGDR
                     QEIAGRRLRLLALDGHTGADLAIYDEASGVLFTGDLGFFGRAPTTPHADVNRWLAALD
                     TLEAITREPGFRAVVPGHGPVARDAAPLRQTRAWLNWLQMALRRSAESGLDMNETMAQ
                     PLPAEFAGLPVAASEYRRSVGHLFPAVEREALGAPR"
     gene            2405..3139
                     /locus_tag="PLN02_06370"
     CDS             2405..3139
                     /locus_tag="PLN02_06370"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOI51985.1"
                     /translation="MRRAETPRPARRLGGLLLLSVLLHGGVFLFIAGSRPASPQPLPQ
                     IIATLRLTQPSNNAVSPAGSAPATPLPQARRLPAAAANPPVPRPPASARPANDASPAT
                     RPAAVPAAGVSSDTAPGPALAAPAVAAAEATPGSVPPAAASAPAAAALAAYRQRLAEL
                     FAQQRSYPRLAAMRGWEGEVRLRLRVARRGNLVAVDVERSSGFDVLDQHARALLASIG
                     DLPPPPGDGGEIQVVVPINYKLDRTT"
BASE COUNT          463 a         1181 c         1161 g          509 t
ORIGIN      
        1 tgttgtcgaa acggacctgg tagccgcgcg cgaccgagcc gctcttgcga tgcagcatgc
       61 cggccgagcg gtcggagctg ccggtggctt cgtacgggtc gatggcgacg aaggccggcg
      121 agtactggcg accgaaggtg acgcgaccga acgatccttc caggccgaga taggattgcc
      181 gggtaaatcc ggtggatgtc ccggtatcga tggcgaccgc cgattcgagc atgaaaatgg
      241 ctttcagacc attgcccatg tcttcgctgc ccttgaagcc gaggcgcgaa ccgttcagtt
      301 cgctggtatc cagcgcgttg cgcttcagac cgttggcgcg ctcctgcgcc agattgacat
      361 cgagcaggcc gaagatggtg acgttcgatt gcgcttgcgc aaggataggg aaaagacagg
      421 cggcagccag tgtggcgagt ttcttcgtgt gttgcatcgt attcctcctt ttttgctgtg
      481 gatgtcgcgg cagcaagcca gtcgccgccg gacctggtgg attaaacgca agctgccttt
      541 cgaagtgctt tcgctcgccg ccggtgaggt gttgcatatg ggagcaactg ctccaggctt
      601 gaacagttcg cccgcgctgt tgccgggcag tcccggccgg ctgggcgttt gcggattggc
      661 acgccacttg caatctcact ccccataaca gcccgcaggg cgagtggagt gagacatgaa
      721 tccaatgaca ggcctggccg ccatcttcct ggcggcgctg ctgactgtcc ggcccgcctt
      781 ggccgggcag gaagacgtcg acccgctggg atcggcgcgc tggggcgaca tgcgttacct
      841 gttcccggag caggcgacgc tgcgcttcga ctcgcgggtc cgcgtgctgg ccccggaagt
      901 cgccgaaaac ccgatgcagg tgccggttac cgtggacgcc agcgagctgg ggccggtcag
      961 cgaggtgatg gtgtgcgccg acttcaaccc catcgtcgag gtgctgcgct tctatccgca
     1021 ggcggcgccg gcctggctgg gctttcgcat caagctgcag cagtcgtcgc cggtgcgggc
     1081 ggcggcgcgt accgccgacg gcgtctggca cgtcggcggt gcctgggtga cgacggtcgg
     1141 cggcggctgt accgcgcctt cggccaaggc cgcggcaccg gactggcagg cgcgcctcaa
     1201 tcaggtcagc ggccggcagt gggagagcgg cgcgcaggcc ggccgggttc gcctgcgcat
     1261 cgagcatccg atggacaccg ggctggtcgc cgggattccc gccttccacc tggaacaggt
     1321 cgaactgctg gccgacggcc gttcgctgat gcgcctgcaa ctgcacgagc cggtcagcga
     1381 gaacccggtg ttcaccctca agcgcggcgc cgccgaaggg ccggtcgagg tacgcggcca
     1441 cgacaacaac ggcaatgcct tccaggcgag gatagcacca tgaagcgctg gctgcccctg
     1501 gctttcctgg gcgtcgcgct ggctgccgcc ggcgccgatt tcgactatcg cctgagcgcc
     1561 gagcgcgtcg ccgacgacgt ccatgtcttc gtcggcaaga ccgaggattt caccccggcc
     1621 aacggcggca atatcgtcaa caccggcttc atcgtcgctc ccggcggcat cgtcgtcatc
     1681 gacagcggcc cgtcgcgccg ctacggtgag cagatgcggc gggcgattgc cgcggtggat
     1741 gggcggccgc tggcgctggt catcaacacc caccatcacc ctgaccattt ccttggcaac
     1801 caggcttacg ccgatgtgcc gctggcggcg ctgcccggca ccatcgaggg catcgccagc
     1861 gacggcaacg ccttcgccga gaatctctac cgcctgaccg gcgactggat ggcggggacc
     1921 gaggtggtcg tgcccaaaca ggcgctggcc ggcgaccgcc aggagatcgc cgggcgccgc
     1981 ctgcgcctgc tggcgctcga cggccacacc ggcgccgacc tggcgatcta cgacgaggcg
     2041 agcggcgtgc tgttcaccgg tgacctcggc ttcttcggcc gggcgccgac gacgccgcac
     2101 gccgacgtga accgctggct ggcggcgctc gacacgctgg aagcgatcac ccgcgagccc
     2161 ggtttccgcg ccgtcgtccc cgggcacggc ccggtggcgc gcgatgcggc gccgctacgg
     2221 cagacccgcg cctggctgaa ctggctgcag atggcgctgc gccgctcggc ggaaagcggg
     2281 ctggacatga acgagacgat ggcccagccg ctgcccgccg agttcgccgg cctgccggtg
     2341 gcggccagcg aataccggcg ctcggtcggc cacctgttcc cggccgtcga gcgggaggcg
     2401 ctcggtgcgc cgcgctgaaa cgccgcggcc ggcgcgccgg ctgggcggcc tgttgctgct
     2461 ctcggtcttg ctgcacggcg gtgtcttcct gttcatcgcg ggcagccggc cggcctcgcc
     2521 gcagccgcta ccgcagatca tcgccacgct gcgcctgacg cagccgtcga acaacgcggt
     2581 gtcaccggcg ggcagtgcgc cggcgacgcc gctcccgcag gctcggcgct tgccggcggc
     2641 cgcggcgaac cccccggttc cgcggccgcc ggcgagcgcc aggccggcca acgacgcgtc
     2701 gccggcaacg cgcccggccg ccgtgccggc ggccggcgtc agcagcgaca cggcgccggg
     2761 gccggcgctt gccgcccccg ccgtcgccgc ggccgaggca acgcccggca gcgtgccgcc
     2821 agccgccgcg tcggcgccgg cggcggcagc gctggccgct taccgccagc gtctcgccga
     2881 gctgttcgcg cagcagcgca gctatccccg cctggcggcg atgcgcggct gggagggcga
     2941 ggtccgtctg cgcctgcgcg tcgcccggcg cggcaacctg gtcgccgtcg acgtcgagcg
     3001 ctcgagcggc ttcgacgtcc tcgaccagca cgcccgcgcg ctgctcgctt ccatcggcga
     3061 cctgccgccg ccgccgggcg acggcggcga aatccaggtc gtcgtgccga tcaattacaa
     3121 actcgataga accacctgag gaagccccga tgtcccgccg tctcacgctc tcaccgcttg
     3181 ccctggcgct cgccgccgcc ttcgccccgg ccgccgccgc cgaaccggtg gtcgaactgc
     3241 cggccgtcga agtggtcggc aattcgccgc tcgccggcat cggcatcccg cgcctgcagg
     3301 taccggccaa cgtg
//
LOCUS       DAOSDX010000238         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10189_length_3309_cov_2.911186, whole genome shotgun sequence.
ACCESSION   DAOSDX010000238 DAOSDX010000000
VERSION     DAOSDX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10189_length_3309_cov_2.911186"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..2067
                     /locus_tag="PLN02_06375"
     CDS             151..2067
                     /locus_tag="PLN02_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI51986.1"
                     /translation="MTTLPARPRLLSLIPPLTQLNTPYPATAYLTGFLRSRGFHATQA
                     DLALETVLELLSPAGLDQLRAAAEALPRKQRSPTIKTFLNQFERYRVATGPALALLQG
                     RDGSLAHRICTRAFLPEGPRFSALDHYVDDGSGDPLGWAFGALGIQDRARHLATLFLN
                     DLADVIREAADPRFEFVRYAESLAQSQPSFEPLAQALAAPPTLVDTTLEKLAISTVDR
                     ENPDILLLSVPFPGSVYAAFRIAQAVKAKFPQIVIVLGGGWVNTELRELAEPRVFDYV
                     DYVTLDDGEAPLLALIEHLAGERPRLNLLRTFTRVDGAVRWFTNGEPDVPFEDVGTPT
                     WDGLPLARYLSLLDMLNPMHRLWSDGHWNKLTVAHGCYWKKCSFCDVSLDYISRFEAA
                     NASTLVDRIERIVDETGHSGFHFVDEAAPPKALRALAEELQRRKLAISWWGNVRFEKS
                     FTPELCLQLADSGCIAISGGLEVASDRLLALMKKGVSVDQVARVTRAFTDAGVLVHAY
                     LMYGFPTQTVQDTVDALEYVRQLFAEGCIQSGFFHRFACTVHSPVGRNPAEYGITLKP
                     LPFKGFAKNDVAFFDPTGVDHTSLGRALNKALYNYMHGICLDDDIRRWFDERVPKPRV
                     KRDFIARALGAVNG"
     gene            complement(2122..2955)
                     /locus_tag="PLN02_06380"
     CDS             complement(2122..2955)
                     /locus_tag="PLN02_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI51987.1"
                     /translation="MFKHKLFSALALLSVPFMLAACVTPGAGGSVTTGSPDATSVSGS
                     ASGSTSVGANAGLERCAKPLGTLAFDDGRGKEWYGSFGRATKVTRIDPLIRLAVQQSN
                     CFVITTIGNSRTESKVSAIADKQRNSGEFRVGSNQHKGQRVAADYYMEPSIVIDESPA
                     GRVAGALGGLIGHGGAIIAAGAESKVSVVTLTLYDIRSGVQLAAAEGNATASNFGAAL
                     GAFGGRAAGGLGGFSQTPEGKATVAAFSDAYNKMVIGLRNYKAQSVEGGLGRGGSLQV
                     N"
     gene            complement(2976..>3309)
                     /locus_tag="PLN02_06385"
     CDS             complement(2976..>3309)
                     /locus_tag="PLN02_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HOI51988.1"
                     /translation="AWMRDAASRGYLPAQTALGRVYLGGLEEMGADPAEAEKWLLMAS
                     GRGDKEAAKLLIDAQKAKQSEVAYQRWKDSQREQSRNFWLGMYEYQWQWRDDDRWHER
                     HVSRSRRY"
BASE COUNT          563 a         1192 c          995 g          559 t
ORIGIN      
        1 cgcctacggc acgcagcgcg cgctcgagca gtacaacaag cagaagctgg ccctcggctc
       61 ggccagcagt taagaccaga cacaacgaca cggggcggcc ggcgggccgc cccgtgtcgt
      121 ttaccggcgg cagcgggtaa actgccggcg atgaccaccc tgcctgcccg cccccgcctg
      181 ctgtcgctga ttccgccgct gacgcaactg aacacgccct acccggcgac ggcctacctg
      241 accggcttcc tgcgttcgcg cggcttccac gcgacgcagg ccgatctggc gctggaaacg
      301 gtcctcgaac tgctctcacc ggccggcctc gaccagttgc gggcggcggc ggaagcgctg
      361 ccgcgcaagc agcgctcgcc gacgatcaag acctttctca accagttcga gcgctaccgc
      421 gtcgccaccg gcccggcgct ggccttgctg cagggccgcg acggctcgct ggcgcaccgc
      481 atctgcacgc gcgccttcct gcccgaaggg ccgcgcttct cggcgctcga ccactatgtc
      541 gacgacggca gcggtgatcc gctcggctgg gccttcggcg cgctcggcat ccaggaccgc
      601 gcccgccacc tggcgacgct cttcctcaac gacctcgccg acgtcatccg cgaggccgcc
      661 gacccccgtt tcgagttcgt ccgctacgcc gaatcgctgg cccagagcca gcccagcttc
      721 gagccgctgg cgcaggcgct ggcggcgccg ccgacgctgg tcgacacgac gctggaaaaa
      781 ctggcgattt cgacggtcga ccgtgaaaat ccggacattc tgctgctctc ggtacccttc
      841 cccggctcgg tctatgccgc cttccgcatc gcccaggcgg tcaaggcgaa gttcccgcag
      901 atcgtcatcg tcctcggcgg cggctgggtc aataccgagt tgcgcgaact ggccgagccg
      961 cgcgtcttcg actatgtcga ctacgtcacc ctcgacgacg gcgaagcgcc gctgctggcg
     1021 ctgatcgagc acctggccgg ggaacggccg cgcctcaacc tgctgcgcac cttcacccgc
     1081 gtcgacggcg ccgtgcgctg gttcaccaac ggcgagccgg atgtgccctt cgaggatgtc
     1141 ggcacgccga cctgggacgg cctgccgcta gcccgttacc tgtcgctgct cgacatgctc
     1201 aatccgatgc accggctgtg gtcggacggc cactggaaca agctgaccgt cgcccacggc
     1261 tgctactgga aaaaatgcag cttctgcgac gtcagcctcg actacatctc gcgcttcgaa
     1321 gcggccaacg ccagcacgct ggtcgaccgc atcgagcgca tcgtcgacga gaccggccac
     1381 agcggtttcc atttcgtcga cgaggcggcg ccgccgaagg cgttgcgcgc gctggccgag
     1441 gaactgcagc ggcgcaagct ggccatttcc tggtggggca acgtccgctt cgagaagtcg
     1501 ttcacgcccg aactctgcct gcagctcgcc gacagcggct gcatcgccat ttccggtggt
     1561 ctggaagtcg cttccgaccg cctgctggcg ctgatgaaaa aaggcgtctc ggtcgatcag
     1621 gtggcgcgcg tcacccgcgc cttcacggac gccggcgtcc tggtccatgc ctacctgatg
     1681 tacggcttcc cgacgcagac cgtgcaggac acggtcgatg cgctggaata cgtgcgccag
     1741 ttgttcgccg agggctgcat ccagtccggc ttcttccacc gcttcgcgtg caccgtgcat
     1801 tcgccggtcg ggcgtaatcc cgcggaatac ggcatcacgc tcaaaccgct gccgttcaag
     1861 ggcttcgcca aaaacgacgt ggccttcttc gatccgaccg gcgtcgatca caccagcctc
     1921 ggccgggcgc tgaacaaggc gctttacaac tacatgcacg gtatctgtct cgacgacgat
     1981 atccgccgtt ggttcgacga gcgggtgcca aaaccacggg tcaagcgcga tttcatcgcc
     2041 cgtgcgctgg gcgccgtaaa cggctgaacg ccggtcgctc cctcgacgag ggccaccggc
     2101 gtccggacca accgacgcgc ctcagttcac ctgcaggctg ccaccacgcc cgaggccacc
     2161 ttcaacgctc tgcgccttgt aattgcgcag gccgatcacc atcttgttgt aggcatcgga
     2221 aaatgccgcc accgtcgcct tgccttccgg cgtctgcgag aatccgccga gaccaccggc
     2281 cgcccgacca ccgaaagccc ccagcgcggc accgaaattg ctcgccgtcg cgttgccttc
     2341 cgcagccgcc aactggacac cggaacggat gtcgtacaag gtcagggtaa cgacggaaac
     2401 cttgctttca gccccagccg cgatgatcgc gccaccatgg ccgatcagac cgcccaaggc
     2461 gccggcgacg cgaccggctg gcgattcgtc gatgacgatc gaaggctcca tgtagtagtc
     2521 ggcggcaacg cgttgcccct tatgctggtt ggaaccgacg cgaaactcac cggagttgcg
     2581 ctgcttgtcg gcgatcgccg aaaccttgct ctcggtccgg ctgttgccga tcgtggtgat
     2641 cacgaaacag ttcgattgct gcaccgccag gcgaatcagc ggatcgatcc gggtcacctt
     2701 ggtggcgcga ccgaaactac cgtaccactc cttgccgcga ccatcgtcga aagccagcgt
     2761 gcccagcggt ttggcgcaac gctcgaggcc ggcattggcg ccgacgctgg tcgaaccgct
     2821 ggccgaaccg ctgaccgagg tggcatcggg cgaaccggtc gtcaccgaac cgccggcgcc
     2881 gggcgtcaca caggccgcga gcatgaacgg caccgacaac agggccaggg ccgagaacaa
     2941 tttgtgtttg aacattttca gcactcctgc aagactcaat aacgacggga tctggataca
     3001 tggcgctcgt gccagcgatc gtcatcgcgc cattgccact ggtattcgta catgcccagc
     3061 cagaaattcc gggattgctc gcgctggcta tctttccagc gttgataagc cacttcgctc
     3121 tgcttggcct tttgcgcatc gatcagcaat ttggcggcct ccttgtcgcc gcggccggac
     3181 gccatcagca accatttttc cgcctcagcc ggatcggcgc ccatttcctc caggccgccc
     3241 aggtaaaccc gaccgagcgc cgtctgcgcc ggcagatagc cgcgcgacgc agcgtcgcgc
     3301 atccaggcc
//
LOCUS       DAOSDX010000239         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10204_length_3306_cov_3.290372, whole genome shotgun sequence.
ACCESSION   DAOSDX010000239 DAOSDX010000000
VERSION     DAOSDX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10204_length_3306_cov_3.290372"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PLN02_06390"
     CDS             complement(<1..743)
                     /locus_tag="PLN02_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI51989.1"
                     /translation="MLKLLVFLPVVGAALLAVAPARAQLQWRLALLASLLALLLVGGL
                     LLRFDPAGAAVQMYESQAWNVRLGSYFALGVDGISLAMLTLSAVLTLVAVIVSRGIPG
                     GGKLYYCLLLLLESAMFGVFTARDWSLFYVFWEATLIPLFFMIDRLGGDNRQRAALNF
                     FLYTLGGSVFMLVALLFLYDAAPGHSFAMHDMAQGGRSLPLPLQLLIFAGLVIGFAVK
                     MPVVPLHGWLPLAHVEAPSPVSILLSGVLL"
     gene            906..1490
                     /locus_tag="PLN02_06395"
     CDS             906..1490
                     /locus_tag="PLN02_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HOI51990.1"
                     /translation="MADNEFLSTRQAAQRLGVSLGTVQNMVENGVLDAWKTAGGHRRI
                     PASSVDALLAKRRKQIARNGDAAGQLDILIAEDDPTLQKLYRMTFDSWKLPVTLRIVG
                     DGFEGLLQVGQRMPDILIADLMMPGMDGFEMIRRLRAMPELSPMDIIVVSAIDAQEIR
                     QKEMPQDVTVFGKPIPFHEIKGFLLGRLAARQRT"
     gene            1593..3110
                     /locus_tag="PLN02_06400"
     CDS             1593..3110
                     /locus_tag="PLN02_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI51991.1"
                     /translation="MGWFGNSASLEKVDRDIAAIADGTADLAQPVGSGGGDIAGRIAG
                     NLNRFFTRIRGLITHARNSSVSIAADAARMNHLVQLTGDAVRRQEVLAEDIFASSNRV
                     NQAVGDVASNADAIQASTQNNLEMAQRSLSQMESVANTMRSTNEHIEHFSTTVVELHS
                     SSMKINEIVSLINDISDQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEKVKTAT
                     LVIGQNTQSMIDLVADTSTRTQTIVGEVTRANDHIGTSARDLGSMVGDFKRTTEQLST
                     IASAIYKLRESNQAIHREVEEIRNHSVDISGRMKQCQDSAKTLRESTEDLQCTLADFR
                     TGNSMFDTLHDRCIGFRDAVAAILQRLAERGVNVFDQAYKEIPGSNPKRYTTVYDAQC
                     DKELTSLYDRLLGELPGLVYSLAVDSNGYAPAHNGNFSHPPSGDPAVDLAKCRHKRLF
                     NDPVGIKLARNQKSSLFQTYVRDTGEILNDLSMPISINGRHWGAVRIGFKSELVA"
     gene            complement(3107..>3306)
                     /locus_tag="PLN02_06405"
     CDS             complement(3107..>3306)
                     /locus_tag="PLN02_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin fold metalloprotein, WbuC family"
                     /protein_id="HOI51992.1"
                     /translation="GVELAAGVYHSVVALDTGTVIFEVKAGPYNPALDVERATWAPAE
                     SAPAACDYLAGLHALFAANKT"
BASE COUNT          704 a         1108 c         1018 g          476 t
ORIGIN      
        1 agcaggacgc cggaaagcag gatggacacc gggctcggcg cctcgacgtg ggccagcggc
       61 aaccagccgt gcagcggaac gaccggcatc ttgaccgcga aaccaatgac cagcccggca
      121 aagatcagca gttgcagtgg cagcggcagg ctgcgcccgc cctgcgccat gtcgtgcatg
      181 gcgaaactgt ggccgggcgc cgcgtcatag aggaaaagca gcgccaccag catgaacacc
      241 gaaccgccca gggtatagag gaagaagttg agtgccgcgc gttgccggtt gtcgccgccg
      301 agacggtcga tcatgaagaa cagcggaatc agcgtcgctt cccagaagac gtagaagagc
      361 gaccagtcgc gcgcggtgaa cacgccgaac atcgccgatt cgagcagcaa cagcaggcag
      421 taatagagct tgccgccgcc cggaatgccg cgcgagacga tcaccgcgac cagcgtcagg
      481 acggcgctca gcgtcagcat ggcgagcgag atgccatcga cgccgagcgc gaaataggaa
      541 ccgaggcgga cgttccaggc ctggctctcg tacatctgga cggcggcgcc ggccggatcg
      601 aagcgcagca gcaggccgcc gaccagcagc aaggccagca gcgacgccaa cagcgccagc
      661 cgccattgca gttgcgcccg tgccggcgcc acggccagca gggcagcgcc gacaaccggc
      721 aggaaaacca gcagcttgag cacccccgcc ccccgctccc gtgattatga atttttgatg
      781 cgcggattat gccagtctcc tcggcggctt tggcaaaatc ggaaaatccg gctgcctgag
      841 attctttaca caaaggtaac ggctttcggt tacaatttgg caaatttgca attttggcga
      901 caacaatggc agacaacgaa tttctcagca cccgtcaggc cgcgcaacgt ttgggcgtct
      961 ccctgggcac ggtccagaac atggttgaaa acggcgtcct cgatgcctgg aagacggccg
     1021 gcggtcatcg ccggattccg gccagctcgg tggatgccct gctcgccaag cggcgcaagc
     1081 agatcgcccg caacggcgac gccgccggcc agctcgacat cctgatcgcc gaagacgatc
     1141 cgaccttgca gaagctctac cggatgacct tcgacagctg gaagctgccg gtgaccctgc
     1201 gcatcgtcgg cgacggcttt gaaggcctgc tgcaggtcgg gcagcgcatg ccggacatcc
     1261 tgatcgccga tctgatgatg ccgggcatgg acggtttcga aatgatccgc cgcctgcgcg
     1321 cgatgcccga actgagcccg atggacatca tcgtcgtcag cgccatcgac gcccaggaaa
     1381 tccgccagaa ggaaatgccc caggacgtca ctgtcttcgg caaaccgatt cccttccacg
     1441 aaatcaaggg tttcctgctc ggccggctgg cggcgcggca gcgcacctga cgcgacacac
     1501 cattccggaa tagatcgccc acaagggtga caggaaggcc cacgagaggc attcccgacg
     1561 gagagcagcg caaatcggag gaggaaacat caatgggctg gtttggcaat tcggcatcgc
     1621 tggaaaaggt tgatcgggac attgcggcga ttgccgatgg caccgccgat cttgcccagc
     1681 cggtcggcag cggcggcggc gatatcgccg gacgcatcgc cggcaatctc aaccgcttct
     1741 tcacccgcat ccgcggcctg atcacgcatg cccgcaacag cagcgtcagc atcgccgccg
     1801 acgcggcgcg catgaaccac ctggtgcaac tgaccggcga cgcggtacgc cgccaggaag
     1861 tgctggccga ggacatcttc gcctcgagca accgggtcaa ccaggcggtc ggcgacgtcg
     1921 ccagcaacgc cgacgcgatc caggcgtcga cgcagaacaa tctggaaatg gcgcaacgct
     1981 cgctgagcca gatggaatcg gtcgccaaca cgatgcgctc gaccaacgag cacatcgagc
     2041 acttctcgac gacggtcgtc gaactgcata gcagttcgat gaagatcaac gagatcgtct
     2101 cgctgatcaa cgacatttcc gaccagacca acctgctcgc cctcaacgcg gcgatcgagg
     2161 ccgcccgcgc cggcgaggcc ggccgtggct tcgccgtcgt cgccgacgag gtgcgcaagc
     2221 tggccgagaa ggtcaagacg gcgaccctgg tgatcggcca gaacacgcag tcgatgatcg
     2281 acctggtcgc cgacacctcg acccggacgc agaccatcgt cggcgaggtc acccgggcca
     2341 acgaccatat cggtacttcg gcacgcgacc tcggcagcat ggtcggcgac ttcaagcgca
     2401 ccaccgagca gttgtcgacg atcgccagcg cgatctacaa gctgcgcgag agcaaccagg
     2461 cgatccaccg cgaggtcgag gaaatccgca accattcggt cgacatctcc ggacgcatga
     2521 aacagtgtca ggattcggcg aagacgctgc gcgaatcgac cgaagatctg caatgcacgc
     2581 tcgccgactt ccgcaccggc aacagcatgt tcgataccct gcacgaccgc tgcatcggct
     2641 tccgcgacgc cgtcgccgcc atcctgcagc gcctcgccga gcgcggcgtc aatgtcttcg
     2701 atcaggccta caaggaaatc cccggctcca acccgaagcg ctacacgacc gtttacgacg
     2761 cccagtgcga caaggaactg accagcctct acgaccgcct gctcggcgag ctgcccggcc
     2821 tcgtctattc gctggccgtc gacagcaacg gctacgcgcc ggcccacaac ggcaacttct
     2881 ctcacccgcc cagcggcgac cccgcggtgg acctggcgaa gtgccggcac aagcgcctgt
     2941 tcaacgaccc ggtcggcatc aagctggcgc gcaaccagaa atcctcgctg ttccagacct
     3001 atgtccgcga caccggcgaa atcctcaacg acctgtcgat gccgatcagc atcaacggcc
     3061 gccactgggg cgccgtgcgc atcggcttca agagcgaact ggtcgcttaa gtcttgttgg
     3121 cggcaaagag ggcgtgcagg ccggccaggt agtcgcacgc cgcgggcgcc gactcggccg
     3181 gcgcccaggt agcgcgctcg acatcgagcg ccgggttgta gggaccggcc ttgacttcga
     3241 agatgacggt tccggtgtcg agcgcgacga cgctgtgata gacgccggcg gccagctcga
     3301 cgccga
//
LOCUS       DAOSDX010000240         3301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10223_length_3301_cov_2.511707, whole genome shotgun sequence.
ACCESSION   DAOSDX010000240 DAOSDX010000000
VERSION     DAOSDX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10223_length_3301_cov_2.511707"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLN02_06410"
     CDS             complement(<1..317)
                     /locus_tag="PLN02_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HOI51993.1"
                     /translation="MDTLLYWVGMAAVAVYALTGVLDSARKRMDLIGALLVAVATALG
                     GGTVRDLLLDRQVFWVVDQTYLMAALSTGILAFILVRHRRLPASLFLIPDAIGLALFT
                     IVGT"
     gene            complement(329..778)
                     /gene="smpB"
                     /locus_tag="PLN02_06415"
     CDS             complement(329..778)
                     /gene="smpB"
                     /locus_tag="PLN02_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOI51994.1"
                     /translation="MSITVNKKAFHDYFIEEKYEAGIVLEGWEVKAIRAARMNIKESY
                     VIIRHGELFLIGMHITPLSTASTHNRHDPVRTRKLLLHAREIGKLIGKVERAGYALVP
                     LDLHFVRGRIKLEIGLAKGKKQHDKRADQQEKEGKREAQRAMKEKLR"
     gene            839..1282
                     /locus_tag="PLN02_06420"
     CDS             839..1282
                     /locus_tag="PLN02_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HOI51995.1"
                     /translation="MAQVQKTVLIEYSALQMFELVDRCEDYPAFLPWCGRTELKHRDE
                     RKTVATLHINYHAVKSSFTTENDKEIPTLMKIRLVDGPFRRLEGAWHFRPLAEHACKI
                     EFHLHYEFSSKLFEKVIGPVFSHIANTFVDAFVRRAAQVYGASHG"
     gene            1275..1595
                     /locus_tag="PLN02_06425"
     CDS             1275..1595
                     /locus_tag="PLN02_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOI51996.1"
                     /translation="MADEMINVEVCYPLPARQEVIRLRLAPGATLQQAVEASGLLAKY
                     PEIDLQKNKFGIWNKLSKLDAALRDFDRVEIYRPLIADPKEVRKQRAAEGKAMKKGAG
                     ETDA"
     gene            complement(1617..2120)
                     /locus_tag="PLN02_06430"
     CDS             complement(1617..2120)
                     /locus_tag="PLN02_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOI51997.1"
                     /translation="MNKALFAALLVAPVVMLSAAHGQTYQWKDSSGKTVISDSPPPGS
                     AKASRSIGAKQPAVVTGSAETESEKGKTDKAAASAPQTIAEKELEFRKRQQESREKAE
                     KEEKEAAAAREKRDTCERARNNYRLLQSDTPLSQPNGQGRLQTMDAAKRSQELERTRR
                     IMQEACK"
     gene            2188..>3301
                     /gene="guaB"
                     /locus_tag="PLN02_06435"
     CDS             2188..>3301
                     /gene="guaB"
                     /locus_tag="PLN02_06435"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOI51998.1"
                     /translation="MRLLQKALTFDDVLLVPAHSQVLPRDVSLSTRLTRNITLNLPLL
                     SAAMDTVTEGRLAIAMAQEGGIGIIHKNLSSRAQAAEVAKVKRFESGILRDPITVTPN
                     MTVRDVLEITRQHRISGLPVIDKTGKVVGIVTNRDLRFETNLDQPVKSIMTPRKRLVT
                     VGEEAGVEEAKELIRTHRLERVLVVNDAFELRGLITVKDILKSTEHPLANKDATGRLR
                     AGAAVGVGAGTEERVELLAEAGVDVVVVDTAHGHSQGVLDRVRWVKQNFPQIQVIGGN
                     IATAGAALALVDAGADGVKVGIGPGSICTTRIVAGVGVPQITAIQNVSEALKGTGVPM
                     IADGGIRYSGDIAKAIAAGADTVMLGGLFAGTEEAPG"
BASE COUNT          599 a          990 c         1018 g          694 t
ORIGIN      
        1 gtaccaacga tggtaaacag cgcgagaccg atggcatccg ggatcaggaa caggctggct
       61 ggcaggcggc ggtggcggac gaggatgaag gcaaggatac cggtactcaa ggcagccatc
      121 agataggtct ggtcaaccac ccagaagact tggcggtcga gcagtaggtc gcgcacggtg
      181 ccgccgccca gtgcggtggc gacggcgacc agcagggcgc cgatcaggtc catccgcttg
      241 cgcgccgagt cgagcacgcc ggtcagcgca tagacggcaa ccgccgccat gccgacccag
      301 tagagcaggg tatccatgct cattgcgctc agcgcagttt ttccttcatc gcccgttggg
      361 cctcgcgttt gccttccttc tcttgttggt cggcacgttt gtcatgttgc tttttgccct
      421 tggcgaggcc gatttcgagc ttgatgcggc cgcggacgaa gtgcaggtca agagggacca
      481 aggcgtagcc ggcgcgctcg accttgccga tcagtttgcc gatttcgcgg gcgtgcagga
      541 ggagcttgcg ggtgcgcacc gggtcgtgcc gattgtgcgt ggaggcggtc gatagcggcg
      601 tgatgtgcat gccgatcagg aacaactcgc cgtggcggat gatcacgtag gattccttga
      661 tgttcatccg ggcggcgcgg atcgctttga cttcccagcc ttcgagcacg atgccggcct
      721 cgtacttctc ttcgatgaag tagtcgtgaa aggctttttt attgaccgtg atgctcatgc
      781 cgccgcgcgt tccattaaaa tccgcgattc tacaggatta caggaatacc gctgcagcat
      841 ggcccaggtc caaaaaaccg tcttgatcga atactcagcc ttgcagatgt tcgaactggt
      901 cgaccgttgc gaggactatc ccgccttcct gccgtggtgt ggtcgcaccg aactgaagca
      961 ccgcgacgag cggaaaacgg ttgccaccct gcacatcaac taccatgccg tgaagtcgag
     1021 tttcaccacc gaaaacgaca aggaaatccc gaccttgatg aagattcgtc tggttgacgg
     1081 accttttcgt cggctcgaag gggcctggca tttccggccg ctcgccgagc atgcctgcaa
     1141 gatcgaattt cacctccact acgagttttc cagcaagttg ttcgagaagg tgatcggccc
     1201 agtcttcagc catattgcca acacttttgt cgatgccttc gtgcgtcgtg ccgcacaggt
     1261 ttatggagcc agtcatggct gatgaaatga tcaacgtcga agtctgctat ccgctgccgg
     1321 cgcgccagga agtgatccgc ctgcgtctgg cgccgggcgc gacgctgcag caggcagtcg
     1381 aggcttccgg gctgctggcg aagtatccgg aaatcgatct ccagaaaaac aagttcggta
     1441 tctggaacaa gctttcgaag ctcgacgctg cgcttcgcga cttcgatcgc gtggaaatct
     1501 atcggccgtt gattgccgat ccgaaggagg tgcgcaagca gcgcgccgct gaaggcaagg
     1561 ccatgaaaaa aggcgccggg gaaaccgacg cctgattgcg ctgaacttgc cgtttcctac
     1621 ttgcaggctt cctgcatgat acggcgggtg cgttcgagtt cctggctacg ttttgcggca
     1681 tccatggtct gcagcctccc ctgaccattc ggttgggaaa gtggtgtgtc ggattgcagc
     1741 aaacgataat tgttgcgggc acgttcgcag gtgtcgcgtt tctcacgggc ggcggcggct
     1801 tccttttctt ccttttccgc tttttcccgg gattcctgct ggcgcttgcg gaattccagt
     1861 tccttttcgg cgatggtttg cggggcgctt gccgcagcct tatcggtctt gcccttttcg
     1921 gattcggttt ctgcgctgcc ggtgactacc gccggctgct tagcaccgat cgagcggctg
     1981 gctttggctg aaccgggggg gggtgagtcg gaaatcacgg tcttgccgct gctgtccttc
     2041 cattggtagg tttgcccgtg ggcagcgctg agcatgacga cgggggcgac caatagtgcg
     2101 gcaaacaggg ctttgttcat gattggctcg tgaaggcgtg gctcggattt ctgtataata
     2161 cgattttgtg accttggatc aaaggccatg cgacttctgc aaaaagccct cactttcgac
     2221 gacgtcctgc tcgtccccgc ccattctcaa gttctgccgc gcgatgtcag cctgtctacc
     2281 cgactgacgc gcaacatcac cctgaatctg ccactgcttt ccgccgccat ggataccgtg
     2341 acggaaggtc gtctggccat cgccatggct caggaaggcg gcatcggcat catccacaag
     2401 aatctctcgt cccgcgccca ggctgccgaa gtggccaagg tcaagcgttt cgaatcgggc
     2461 atcctgcgcg atccgatcac cgtgacgcca aacatgacgg tgcgcgatgt gctcgagatc
     2521 acccgccagc accgcatttc cggcctgccg gtgatcgata agaccgggaa ggtggtcggc
     2581 atcgtcacca accgcgacct gcgcttcgag accaatctcg accagccggt gaagtcgatc
     2641 atgacgccgc gcaagcgtct ggtcaccgtc ggcgaggaag ccggcgtcga ggaagccaag
     2701 gaactgatcc gcacgcatcg tctggagcgt gtgctggtgg tcaatgacgc tttcgaactg
     2761 cgcggcctga ttaccgtcaa ggacattctc aagtccaccg aacatccgct cgccaacaag
     2821 gacgctaccg gtcgcctgcg cgccggtgcg gcggtcggcg tcggtgccgg taccgaggaa
     2881 cgtgtcgaac tgctggccga ggccggcgtc gatgtcgtcg tcgtcgatac cgcccacggt
     2941 cactcgcagg gcgtgctcga tcgcgtgcgc tgggtgaagc agaatttccc gcagattcag
     3001 gtcatcggcg gcaacatcgc caccgctggc gctgcgctgg cgctggtcga tgccggcgcc
     3061 gacggcgtca aggtcggcat cggccccggc tctatctgca ccacgcgtat cgtcgctggt
     3121 gtcggcgtgc cgcagatcac cgccatccag aacgtttccg aagcgctcaa gggtactggc
     3181 gtaccgatga tcgccgacgg cggcatccgc tactccggcg acatcgccaa ggcgattgcc
     3241 gctggtgccg acacggtcat gctcggcggc ctgttcgccg gcaccgagga agcccccggc
     3301 g
//
LOCUS       DAOSDX010000241         3300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10229_length_3300_cov_3.308166, whole genome shotgun sequence.
ACCESSION   DAOSDX010000241 DAOSDX010000000
VERSION     DAOSDX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10229_length_3300_cov_3.308166"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLN02_06440"
     CDS             complement(<1..1125)
                     /locus_tag="PLN02_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOI51999.1"
                     /translation="MSSALWLALLCAGLAVIYGWLSSRSILALPAGNERMQEIAKAIQ
                     EGAAAYLSRQYKTIAMVGVVMAVLIAIFLGMPTAVGFVIGATLSGAAGFIGMNVSVRA
                     NVRTAEAARGGIAPALDVAFKGGAITGMLVVGLGLLGIAGYYGGMQAFGLSQDAALHA
                     LIGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDN
                     VGDCAGMAADLFETYVVTVVATMVLGALMLKTSAALGDALILYPLALGAVSIIASIVG
                     CYFVKTTDGGKIMGALYKGLIVAGVLALAFYYPVTSWLIGAGDTAVQVTTASMFTCAV
                     IGLVLTAAMVWITEYYTGTEYAPVKHVASACQTGHAPNIIA"
     gene            complement(1722..2060)
                     /locus_tag="PLN02_06445"
     CDS             complement(1722..2060)
                     /locus_tag="PLN02_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOI52000.1"
                     /translation="MKKIEAIIKPFKLDEVREALSEVGITGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKIEIVVAGPQVDPAIDAIVKAARTGKIGDGKIFVTSVEQVVRI
                     RTGETNEAAI"
     gene            complement(2092..>3300)
                     /locus_tag="PLN02_06450"
     CDS             complement(2092..>3300)
                     /locus_tag="PLN02_06450"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOI52001.1"
                     /translation="GVRCGINICADVWETGAFEAACQAGAEIVLVLNASPCHLEKQQE
                     RAEVLGERIAATGIPAIYCNLVGGQDELVFDGGSFALDARRQVAMRLPQFAEALGIVD
                     VDGPRLHSTDLVAPLSLEAEAYSALVMGVRDYIGKSGFKGAIIGLSGGIDSALTLCVA
                     VDALGADKVHAVMMPSPYTAQMSLDDSREMIRQLGCSYDEIAIAPAMATYEAMLAPLF
                     AGLPCDTTEENIQARIRGNLLMALSNKTGKLVLTTGNKSEMAVGYCTLYGDMAGGFAV
                     IKDVYKTLVYRLARYRNTLCSSDPVIPENIIVRPPSAELKPDQKDQDSLPEYEVLDAI
                     VRAYMEEDRSPREIIAAGLPEADVRRVVRLLRIAEYKRRQAPVGIRITPRGFGKDWRY
                     PITNRYADEY"
BASE COUNT          636 a         1053 c         1026 g          585 t
ORIGIN      
        1 ggcgatgatg ttgggcgcat gaccggtctg gcaagccgaa gcgacatgct tgaccggcgc
       61 gtattccgtc ccggtgtagt actcggtgat ccagaccatc gccgcggtca gcacgaggcc
      121 gatgacggca caggtgaaca tgcttgccgt cgtcacctgt actgccgtgt caccggcacc
      181 gatcaaccac gaggtcaccg ggtagtagaa ggccagcgcc agcacgccgg caacgatcag
      241 gcctttgtac agcgccccca tgatcttgcc gccgtccgtg gtcttgacga agtagcaacc
      301 gacgatcgag gcgatgatcg acaccgcgcc cagcgccagc ggatagagga tcagcgcatc
      361 gccgagcgcc gccgaggtct tcagcatcag ggcgccgagc accatggtgg cgacgacggt
      421 gacgacatag gtctcgaaca ggtcggccgc catgccggcg caatcgccaa cgttgtcgcc
      481 gacgttgtcg gcaatcaccg ccgggttgcg cggatcgtcc tcgggaatgc cggcttccac
      541 cttgccaaca aggtcggcgc cgacgtcggc gcccttggtg aagatgccgc cgccgagacg
      601 ggcgaagatc gagatcagcg acgaaccgaa ggcgagaccg atcagcgcgt gcagcgccgc
      661 atcctggctc aggccgaagg cctgcatgcc gccgtagtag ccggcgatgc cgagcagacc
      721 gaggccgacc accagcatgc cggtgatcgc gccgcccttg aaggccacgt caagcgccgg
      781 cgcaatgccc ccgcgtgccg cttcggcggt acgcacattg gcgcggacgg aaacgttcat
      841 gccgatgaaa ccggcggcac cggacaaggt ggcgccgatg acgaaaccga ccgccgtcgg
      901 catgccgagg aaaatggcga tcagcaccgc catcaccaca ccgaccatcg cgatggtctt
      961 gtactgccga ctcaaatagg cggccgcccc ttcctgaatc gccttggcga tttcctgcat
     1021 gcgctcgttc cccgccggca acgcaagaat cgaacgactg gacaaccagc cgtaaatgac
     1081 cgccaacccc gcacataaca acgccaacca caatgcagaa gacattactt ctccctctct
     1141 tttgactgac aaaaattgaa cagactattt ctccgcaata ttttccggac ggcaagacac
     1201 agcccggaca cggatgcgcc tgggctgtta ccggaaaagg aggaaaacgg gtgcttgtcg
     1261 gtctgtgccg aatcgggtct tgtctggaaa accgcgccga ccgcgacagc gcttccgggt
     1321 accgcgtgga aggctggccg cgtcgtcata ctctggccct tagttcaggg acatgacacg
     1381 gccggacata ccgaccctgt catcgccaca tgcctcacgg catgccggga agattgggga
     1441 taggtgacgg cggttgccgt cacatcttct ctctacctga atgatgtaca tgaaatcccg
     1501 cttgccactg aatcgttgga gaatcctgac tgacggatga ggggaacccc ctcaggcgaa
     1561 ggaaattacc cgcttttgcc gggtcggtca aacgatttgc tcagacttgg tgcaaatcgc
     1621 tggcaatgac atgaaaaacc gcctggcatg caccactccg gaaacgaaaa cgggacggct
     1681 gacgccgtcc cgctcgctcc accgcgcctg gtggcaggtg atcagatggc cgcttcgttg
     1741 gtctcgccgg tgcgaatacg aacaacctgt tcgacgctgg tgacgaagat cttgccgtca
     1801 ccgatcttgc cggtgcgcgc cgccttgacg atggcgtcga tcgccggatc gacctgcgga
     1861 ccggcgacga cgatctcgat cttgatcttg ggcaggaaat cgacgacgta ctcggcgccg
     1921 cgatacagct cggtatggcc cttctggcga ccgaagccct tgacctcggt gaccgtcagg
     1981 ccggtgatgc cgacttccga cagcgcctcg cgcacttcgt cgagcttgaa cggcttgatg
     2041 atggcttcga tttttttcat gtctgtatcc ctcttctctc tcgattgatg ctcaatattc
     2101 gtcagcatac cggttggtga tcgggtagcg ccagtccttg ccgaaaccgc gcggcgtgat
     2161 gcggatgccg accggcgcct ggcgccgctt gtactcggcg atgcgcagca ggcggacgac
     2221 gcggcgaacg tcggcttccg gcagaccagc ggcgatgatc tcgcgcgggc tgcggtcctc
     2281 ttccatgtag gcgcgaacga tggcgtcgag cacttcgtat tcgggcagcg aatcctggtc
     2341 cttctggtcc ggcttcagct cggccgacgg cgggcggacg atgatgtttt ccggaatcac
     2401 ggggtcgctg ctgcacaggg tgttgcggta gcgcgccaga cgatagacca gggtcttgta
     2461 gacatccttg atcaccgcga agccgccggc catgtcgccg tacagcgtgc agtagccgac
     2521 cgccatttcc gacttgttgc cggtggtcag gaccagcttg ccggtcttgt tggagagcgc
     2581 catcagcagg ttgccgcgga tccgcgcctg gatgttctct tcggtggtgt cgcagggcag
     2641 gccggcgaat agcggcgcca gcatggcttc gtaagtggcc atcgccggag cgatggcgat
     2701 ctcgtcgtag ctgcagccca actggcggat catctcgcgc gaatcgtcga gactcatctg
     2761 cgcggtgtag ggcgacggca tcatcaccgc gtgcaccttg tcggcgccga gcgcgtcgac
     2821 ggccacgcac agcgtcagcg ccgaatcgat gccgcctgag aggccgatga tcgccccctt
     2881 gaagccactc ttgccgatgt agtcacgcac ccccatgacc agcgccgaat aggcctcggc
     2941 ttccagcgac agcggagcaa cgagatcggt ggaatgcagg cgcggcccgt cgacgtcgac
     3001 gatacccagg gcttctgcaa actgcggcag gcgcatggcg acctggcggc gcgcgtcgag
     3061 ggcgaaggag ccgccgtcga aaacgagttc atcctggccg ccaaccagat tgcaatagat
     3121 ggcgggaatg ccggtggcgg caatcctttc gccgagcacc tcggcgcgct cctgctgctt
     3181 ctccaggtgg cagggcgagg cattgagcac cagcacgatc tccgcaccag cctggcaggc
     3241 cgcttcgaaa gcgccagttt cccagacgtc ggcacagatg ttgatgccgc agcggacgcc
//
LOCUS       DAOSDX010000242         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10242_length_3296_cov_3.684048, whole genome shotgun sequence.
ACCESSION   DAOSDX010000242 DAOSDX010000000
VERSION     DAOSDX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10242_length_3296_cov_3.684048"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1223)
                     /locus_tag="PLN02_06455"
     CDS             complement(180..1223)
                     /locus_tag="PLN02_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI52002.1"
                     /translation="MIQLKSLNKLAAIAAALSVAGAAQAADLTVISFGGTNQKAQVKA
                     YYEPFMRNTGIKIIPGEYNGEQAKVKAMVEAGNVSWDVVELESPEMARGCEEGLYEKL
                     DYSKIGNKKDFLPAAVNECGIGFFVWSTVLAYNADKLKQAPTSWSDFWDMKKFPGKRG
                     MRKGAKFTLEFALMADGVAPADVYKVLATKAGVDRAFKKLDQIKPHIQWWEAGAQPPQ
                     LLAAGDVVMSSAYNGRIATAQKEGRNLKIVWTGNIYDVESYGIPKGAAKREEAYKFLA
                     TLVKPENQVVFSGEIPYGPTNVKANPQISAAIAAELPTSASNIKTAVASNTMFWVEHG
                     EDLEQRFNAWAAR"
     gene            complement(1286..2395)
                     /locus_tag="PLN02_06460"
     CDS             complement(1286..2395)
                     /locus_tag="PLN02_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI52003.1"
                     /translation="MGQQDGGSAHVTFRKVQKTYDGEHLIVRDLNLDIQRGEFLTLLG
                     PSGSGKTTCLMMLAGFETPTAGEICLGDRVINKLPPHKRNIGMVFQNYALFPHMTVAE
                     NLRFPLAIRKLPAAEIDAKVRNALAMVRLEKFAGRYPQQLSGGQQQRIALARALVFGP
                     ELVLMDEPLGALDKQLREHMQLEIKHIHENLGITVVFVTHDQGEALTMSDRIAVFNHG
                     VIQQIDSAEALYERPVNSFVASFIGENNALNGTVERLADGCCQVRLDCGARIEAVARN
                     VSGVGARTMVSVRPERAFLADGEGGGNRFSAGVKEIIYLGDHVRVRLDLVGGHDFTVK
                     MPISQLDRQLRSGDQVVVAMAAEHACALDPVVDAH"
     gene            2535..>3296
                     /locus_tag="PLN02_06465"
     CDS             2535..>3296
                     /locus_tag="PLN02_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI52004.1"
                     /translation="MEARLITELLEQYLDRQSAPLKLRIRIYLALRQAILDRALAPGT
                     KLPPTRSLAEDLGMGRNTVVRAYEQLLVEGYIESRTGDGSYVSEAAQGGLRRSPPKRL
                     LGTRREGLSRRGSIVAANATSSVVQRGAFMPGVPDIDAFPFAIWRRLLGKYHSAEQGH
                     LLNYAHTGYGPLKVMLANYLRVTRMMPVDPKQVIIINGSHQAIDLCARLLADHGDRAW
                     IEDPGYWGARNVLVAAGLQVTPIPLDAKGLAPSVED"
BASE COUNT          565 a         1066 c         1053 g          612 t
ORIGIN      
        1 atcaaggcac cgtagcgcag cttcttcatg cgttcggcgc gctgcagcag gcgcttgagc
       61 ggaacggctg ccgtttcggc aatcggctcg gacatcgttt ctccctggac catcatcgtc
      121 ttcccttatt ccccgatccg gccgcctccc cgctcgatgc ggggaagcgg ccagttttgc
      181 tagcgcgcgg cccaggcgtt gaaacgctgt tccagatcct cgccgtgctc gacccagaac
      241 atggtgttgc tagcgacggc ggtcttgata ttgctggcgg aagtcggcag ctcggcagca
      301 atcgccgcgc tgatctgcgg gttggccttg acgttggtcg ggccgtaggg aatctcgccg
      361 gagaagacca cctggttttc cggcttgacc agcgtcgcca ggaacttgta ggcctcctcg
      421 cgcttggccg cgcccttcgg aatgccgtag gactcgacgt catagatgtt gccggtccag
      481 acgatcttca ggttgcggcc ttccttctgg gcggtggcga tgcggccgtt gtaggcggag
      541 ctcatcacca cgtcgccggc ggccagcagt tgcggcggct gggcaccggc ttcccaccac
      601 tggatgtgcg gcttgatctg gtccagcttc ttgaacgccc gatcgacacc cgccttggtc
      661 gccagcacct tgtagacgtc ggccggagcg acgccgtcag ccatcagcgc gaactccagc
      721 gtgaacttgg cgcccttgcg catgccgcgc ttgccgggga atttcttcat gtcccagaaa
      781 tcgctccagg aagtcggcgc ctgcttcagc ttgtcggcgt tgtaggcgag cacggtggac
      841 cagacgaaga agccgatgcc gcactcattg accgccgccg gcaggaaatc cttcttgttg
      901 ccgatcttgc tgtagtcgag cttctcgtac agcccttcct cgcagccgcg ggccatttcc
      961 ggcgattcga gttcgaccac gtcccagctg acgttgccgg cttcgaccat ggctttgacc
     1021 ttggcctgct cgccgttgta ttcgccgggg atgatcttga tcccggtgtt gcgcatgaag
     1081 ggctcgtagt aggccttgac ctgcgccttc tgattggtac cgccgaagga aatcacggtc
     1141 aggtcggcgg cctgggccgc accggcgacg ctcagggcgg cggcgatggc ggcaagcttg
     1201 ttcagggact tcagttgaat catggcaaaa ccctcctctg agttattggt gaatcgctgc
     1261 acggcggcgc cgggcaagtt gaaaatcaat gcgcgtcaac caccggatca agcgcgcagg
     1321 cgtgttcggc ggccatcgcc acgaccacct ggtcacccga acgcaactgg cggtcgagct
     1381 gcgagatcgg catcttgacg gtgaaatcgt gaccgccgac gaggtcgaga cggaccctga
     1441 cgtggtcgcc gaggtagatg atttccttga ccccggcgga gaagcggttg ccgccccctt
     1501 cgccgtcggc caggaaggcg cgttcggggc ggaccgaaac catggtccgc gcgccgaccc
     1561 cggagacgtt gcgggccacc gcctcgatcc gcgcaccgca atccaggcgc acctggcagc
     1621 agccgtcggc caggcgctcg acggtgccgt tgagggcgtt gttctcgcca atgaagctgg
     1681 caacgaagga attgaccggc cgctcgtaaa gggcttcggc actgtcgatc tgctggatca
     1741 cgccgtggtt gaagacggcg atgcggtcgg acatggtcag cgcctcgccc tggtcatggg
     1801 tgacgaaaac gacggtgatg ccgaggttct cgtggatgtg cttgatttcg agctgcatgt
     1861 gttcgcgcaa ctgcttgtcg agggcgccga gcggttcgtc catcagcacc agctcgggcc
     1921 cgaagaccag ggcgcgggcc agggcgatgc gctgctgctg gccgccggac aactgctgcg
     1981 gatagcggcc ggcgaatttc tcgagacgga ccatggccag cgcgttcctg accttggcat
     2041 cgatctcggc cgccggcagc ttgcggatgg cgagcgggaa gcgcaggttt tcagccaccg
     2101 tcatgtgcgg gaacaaggcg tagttctgga agaccatgcc gatgttgcgc ttgtgcggcg
     2161 gcagcttgtt gatgacccgg tcgccgaggc agatttcacc ggcggtcggc gtctcgaaac
     2221 cggccagcat catcaggcag gtggtcttgc cggaacccga cggtccgagc agggtgagga
     2281 actcgccgcg ctggatgtcg aggttgagat cgcgaacgat cagatgttcg ccatcatagg
     2341 ttttctgcac cttgcggaag gtaacgtgag cgcttccacc gtcttgctgg cccatggcct
     2401 ctctcctgaa tcacgccttc gccagcaccg ggcaaaccgc ccgctggcac tcgatgggtt
     2461 gatcataggg gaacgattgg tcaaaatgag aaccaatttt tccgaaaaac aaggaaccaa
     2521 ttccggagca caggatggaa gcccgtctga tcaccgagtt gctggaacaa tacctggacc
     2581 gccaatccgc gccgctcaaa ctgcgcatca ggatatacct ggcgctgcgc caggccattc
     2641 tcgaccgggc gctggccccc ggcaccaagc tgccaccgac ccgcagcctg gccgaagatc
     2701 tgggcatggg gcgcaatacc gtggtccgcg cctacgaaca attgctggtc gaaggctata
     2761 tcgaaagccg caccggcgac ggcagctatg tttcggaagc cgcccagggc ggcctgcgcc
     2821 gctcgccgcc gaaacgcctg ctcggcaccc ggcgcgaagg cctgtcgcga cgcggcagca
     2881 tcgtcgccgc caacgccacc agcagcgtcg tccagcgcgg cgccttcatg cccggcgtgc
     2941 ccgacatcga cgcctttccc ttcgccatct ggcggcgcct gctcggcaaa taccacagcg
     3001 ccgaacaggg gcacttgctc aattacgcgc ataccggcta cggcccgctc aaggtgatgc
     3061 tggccaacta cctgcgggtg acgcggatga tgccggtcga ccccaagcag gtgatcatca
     3121 tcaacggctc gcaccaggcg atcgacctct gcgcccgcct cctcgccgat cacggcgacc
     3181 gcgcctggat cgaggacccg ggttactggg gcgcgcgcaa cgtgctggtc gccgccggcc
     3241 tgcaggtgac gccgatcccg ctcgacgcca agggcctcgc gcccagtgtc gaggac
//
LOCUS       DAOSDX010000243         3280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10314_length_3280_cov_3.686512, whole genome shotgun sequence.
ACCESSION   DAOSDX010000243 DAOSDX010000000
VERSION     DAOSDX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10314_length_3280_cov_3.686512"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLN02_06470"
     CDS             <1..1199
                     /locus_tag="PLN02_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52005.1"
                     /translation="LGTVGEWLDRTAEINAQRLVADLIEHAAAGNLEARLDADALEGF
                     YRELGRGINRLLETTGSAVGEIAGLLSRIAAGDLMHTVEADYQGTFGQLKNDANQTVM
                     RLRELVGGIQYSAETINVASKEIASGNQDLSVRTEEQASSLEETASSMEELTATVKQN
                     ASNARQANELAGNAQRIAEQGGEVVGKVVETMGSINQASARIADIIGVIDGIAFQTNI
                     LALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKSLISDSVERVEAGSKLV
                     DQAGATMTEIMSSIKRVARIVTDIAEASREQSAGIEQVSLAISQMDEVTQQNAALVEQ
                     AAAAAESLEEQAESMARGVAVFKLAEGAVPVSKPLALASPTRKPGGRSKNPSPALSLD
                     DEWEEF"
     gene            1201..2115
                     /locus_tag="PLN02_06475"
     CDS             1201..2115
                     /locus_tag="PLN02_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOI52006.1"
                     /translation="MTIEPKRPLPSLGSLRNDLNRVLPKDTGVREFQFSDADFERVRK
                     LIYQHAGISLSPVKKDMVYSRLARRLRALNKHSFTEYLDSLERHGGDEWERFVNSLTT
                     NLTSFFREPHHFPILAEHLRQIGNRRPVRIWCSAASTGEEPYSIAMTVAETCGADASH
                     VSIIASDLDTNVLATAQKGVYAMDRLEKMSPERLRRFFLRGTGGQEGFAAVRPELKRM
                     IEFRRINLLDASYPVKGPLDILFCRNVMIYFDKPTQYQILSRFAPLMQPDGLLFAGHS
                     ESFLHAADLFRSQGKTVYVLAGARQGRA"
     gene            2112..2720
                     /gene="cheD"
                     /locus_tag="PLN02_06480"
     CDS             2112..2720
                     /gene="cheD"
                     /locus_tag="PLN02_06480"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HOI52007.1"
                     /translation="MNHAYNENLAANRYFDRHFSSEAAKILPGEYFVSDQGMLLVTVL
                     GSCVAACIRDVDAGIGGMNHFMLPDDGGRSETVGTSARYGSYAMEVLINHLLKMGARK
                     SRLEAKVFGGGAVMSSLSSSNVGVRNAEFVLNYLKTEKIPVVAKDLLDSYPRKVYYFS
                     QTGRVLVKKLHRVHNETLFSRERDYKERLSGASMGGDVELFI"
     gene            2749..>3280
                     /locus_tag="PLN02_06485"
     CDS             2749..>3280
                     /locus_tag="PLN02_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52008.1"
                     /translation="MKKTRVLIVDDSALMRSLLTTMINGFADLEVVGAAPDASSAREL
                     IKTLAPDVLTLDVHMPGMDGLAFLERLMRLRPMPVVMVSSYTGETSETTLRALELGAV
                     DFIGKPRSDFPNSMEAYAEELAEKIRTAKSARLRGAPAPAASAGIARSSRAMTAAKAT
                     NGASPYSSSNGKIIFLG"
BASE COUNT          640 a          990 c         1043 g          607 t
ORIGIN      
        1 gtctgggtac cgtcggcgaa tggctggacc gcaccgccga gatcaacgcc cagcgactgg
       61 tcgccgactt gatcgagcac gccgccgccg gcaatctgga agccaggctc gacgccgacg
      121 ccctggaagg cttctaccgc gagctggggc gtggcatcaa ccgcttgctg gaaaccaccg
      181 gttcggcggt cggcgagatc gccgggctgt tgtcgcggat cgccgccggc gacctgatgc
      241 acacggtcga agccgattat cagggtacct tcggccagtt gaagaatgac gccaaccaga
      301 ccgtcatgcg cttgcgcgaa ctggttggtg gcatccagta ttccgcggaa acgatcaatg
      361 tcgcctcgaa ggagattgcc tccggcaatc aggatctttc ggtgcggacc gaagagcagg
      421 cgagcagtct cgaggaaacc gcttcgagca tggaagagtt gaccgccacg gtcaagcaga
      481 acgccagcaa cgcccgtcag gccaatgaac tggccggcaa cgcccagcgc atcgccgagc
      541 agggtggtga agtggtcggc aaggtggtcg agaccatggg ctcgatcaat caagccagcg
      601 cgcggatcgc cgacatcatc ggggtaatcg acggcattgc cttccagacc aacatcctgg
      661 ccctcaacgc cgccgtcgaa gcagcgcggg ccggcgaaca gggtcggggc ttcgctgtcg
      721 tcgccaccga agtgcgcagc ctcgcccagc gcagtgctgc cgccgccaag gaaatcaaga
      781 gtctgatttc cgattcggtc gaacgggtcg aagcgggcag caagctggtc gaccaggccg
      841 gggcgacgat gaccgagatc atgagcagca tcaagcgcgt ggcgcggatc gtcaccgaca
      901 ttgccgaagc cagccgcgag cagagcgccg ggatcgagca ggttagcctg gcgatcagcc
      961 agatggacga ggtgacgcag cagaatgccg ccctcgtcga acaggcggcc gccgccgccg
     1021 agagcctgga agagcaggcg gaatcgatgg cgcgtggggt tgccgtgttc aagctggccg
     1081 agggggcggt gccggtatcc aagcccttgg ccctggcttc gccgacacgc aagccggggg
     1141 ggcggtcgaa aaatccgtcg ccggcgctca gtctggacga cgaatgggaa gagttctgac
     1201 atgaccatag agccaaagcg tccgctgccg tccttgggta gcttgcgtaa cgacctcaac
     1261 cgggttctgc ccaaagacac cggggtgcgc gaattccagt tttccgacgc cgatttcgag
     1321 cgcgtgcgca aactgattta tcagcacgcg gggatttccc tgtcgccggt caagaaggac
     1381 atggtctatt cgcggttggc acgccgtttg cgggcgctca acaagcattc cttcaccgag
     1441 tacctggaca gcctggagcg ccatggcggc gacgaatggg agcgtttcgt caactcgctg
     1501 accaccaacc tgacctcctt cttccgcgag ccgcaccatt tccccatcct cgccgaacat
     1561 ctgcgccaga tcggcaaccg gcgaccggtg agaatttggt gctccgccgc gtcgactggc
     1621 gaggaaccct attcgatcgc aatgacagtc gccgaaacct gcggcgccga tgcttcgcat
     1681 gtcagcatca tcgcttccga tctcgatacc aacgtgctgg cgacggccca gaaaggcgtc
     1741 tatgcaatgg accgcctgga aaagatgtcg ccggagcgtc tgcgccgctt cttcctgcgc
     1801 ggcaccggcg gccaggaggg ctttgccgcc gtgcgcccgg agttgaagcg gatgatcgaa
     1861 ttccggcgca tcaacctgct cgacgccagc tatccggtga aggggccgct cgacatcctg
     1921 ttctgccgca acgtcatgat ttatttcgat aagccgacgc agtaccagat cctttcccgc
     1981 ttcgctccgt tgatgcagcc cgatggcctg ctgtttgccg ggcattccga gagcttcctg
     2041 cacgccgccg atcttttccg ttcgcaaggc aagacggttt atgtccttgc cggggctcgc
     2101 cagggacgcg cgtgaatcac gcttataacg aaaacctggc ggccaatcgc tatttcgacc
     2161 ggcatttttc cagcgaagcc gccaagatac tgccgggcga gtacttcgtc tcggatcagg
     2221 gaatgctgct cgtcaccgtg ctggggtcct gcgtcgccgc ctgcattcgc gatgttgatg
     2281 ccggtattgg cggcatgaac catttcatgc tgcccgacga cggagggcgc tccgaaacgg
     2341 tcggcacttc ggcgcgctac ggctcgtatg ccatggaagt cctgatcaac cacctgctga
     2401 agatgggggc gcgcaagagt cgcctggagg ccaaggtctt cggcggaggg gcggtgatgt
     2461 ccagcctgtc gagcagcaat gtcggggtgc gcaacgcgga atttgtgctc aattacctga
     2521 agaccgaaaa aattcccgtc gtcgccaagg acttgctgga ttcctacccg cgcaaggtat
     2581 attacttttc acagacaggg cgcgtgttgg tgaagaagtt gcatcgcgtc cacaacgaaa
     2641 ccctgttcag tcgtgagcgg gattacaagg agagactgtc tggcgcaagc atgggcggcg
     2701 atgtggagct ttttatctga tgtttgatca gtacgatggt ggcggcaaat gaaaaagacg
     2761 cgtgtcctga tcgtggacga ctcggcgttg atgcgctcgc tgctgaccac catgatcaac
     2821 ggttttgccg atctcgaggt ggtcggcgcg gcgcccgatg cgtcgagcgc gcgcgagttg
     2881 atcaagactc tggcgcccga tgtgttgacc ctcgatgttc acatgccggg catggatggc
     2941 ctggcattcc tcgaacgcct gatgcgcttg cggccgatgc cggtggtgat ggtttcctct
     3001 tataccggcg agacatcgga aacgacgctg cgtgcgctgg aactgggcgc cgtcgatttc
     3061 atcggcaagc cgcgctcgga ctttcccaat tcgatggagg cttatgcgga agaactggca
     3121 gaaaagatcc ggacagcgaa aagcgcgcgt ctgcgcggcg ctcctgcgcc cgccgccagc
     3181 gccggcattg ccaggagctc tcgtgcgatg acggcggcca aggctacgaa cggcgcttcc
     3241 ccgtattcga gcagcaacgg aaagatcatc tttctcggtg
//
LOCUS       DAOSDX010000244         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10350_length_3273_cov_3.126165, whole genome shotgun sequence.
ACCESSION   DAOSDX010000244 DAOSDX010000000
VERSION     DAOSDX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10350_length_3273_cov_3.126165"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..944
                     /locus_tag="PLN02_06490"
     CDS             93..944
                     /locus_tag="PLN02_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI52009.1"
                     /translation="MQSLWMIAASFLFACMGVCVKLAAQTHSAVEITFYRSFISLLLM
                     FGLVRLRGVRLATPHWRWQISRGLVGFAALFAYFYAITLLPLATAVTLNYTSAIFLAL
                     YLALAGWQVRRGMLGALALGLAGVILLLKPTFQAEQLVGGLVGLASGVMAGMAYFSVR
                     ELGARGESEIRTVFYFSLVASVCAAVWLVFGEIHPVDADSGLLLFGVAGFATLAQLAM
                     TRAYARGKTLLSAALAYSTVIFSSLAGMLFWGEVLPASAWAAIGLIVLSGIVATHFSR
                     ASPVEQD"
     gene            1040..1459
                     /locus_tag="PLN02_06495"
     CDS             1040..1459
                     /locus_tag="PLN02_06495"
                     /inference="COORDINATES: protein motif:HMM:NF027223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase
                     domain-containing protein"
                     /protein_id="HOI52010.1"
                     /translation="MKVFLGGTCNESTWRRRIIPLLEAEFFNPVVDDWTPDRMAEELR
                     QREECDVCLYVITPKMTGVYSIAEVIDDSNKRPEKTVFVRLRDDDNEKFTDGQWKSLG
                     AVAQMVERNGGRAFDSVKSAATHLSHCCRPECSKEQQ"
     gene            1438..2418
                     /locus_tag="PLN02_06500"
     CDS             1438..2418
                     /locus_tag="PLN02_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HOI52011.1"
                     /translation="MQQGAAMTVLGRLARVDLREVWPHEAYNFTPWMAQSSNLAVLSE
                     ELGLDLELVGQEVAVGPYAADIVCKDIFSGAHVLIENQLEKTDHSHLGQILTYAAALE
                     ARSIVWIASRFTDEHRAAIDWLNEITHDEWQFFGLEIELWRIGDSEPAPKFNMICRPN
                     DWSRAVRDEAAKAGSASPTQALQLKFWSAFRDYMMLKGLRAPKPSAQSWQSFRVGRTG
                     FSIEGVIKRNERQMLVRLYINCDDLPPKAVFRYLRDRRDSVEASMASVMEWNELPDKK
                     GCVIAVVRSNSDLDDETAWPQLHAWLESNVARMEKVFRPLIVSLSAADLA"
     gene            2453..2803
                     /locus_tag="PLN02_06505"
     CDS             2453..2803
                     /locus_tag="PLN02_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOI52012.1"
                     /translation="MIVIDHKAHRVNVSVFGEFTLADYREFEEVVNYKIKFEGPVDLF
                     FDLTKMGTLTLDVAWEEVKFSRAHANDFNRVAVITDSEWVTWSAWLSQTFVNADVQVF
                     DDPAAADEWLEGEA"
     gene            2800..>3273
                     /locus_tag="PLN02_06510"
     CDS             2800..>3273
                     /locus_tag="PLN02_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI52013.1"
                     /translation="MNFGPLIRPAQLLAQLGEPDCCIVDVRHQLADVGYGERAYGEAH
                     LPGAFFLHCDRDLSGPLNGRNGRHPLPDPKVLAGRLAALGIGRQTRVVAYDDGNGMIA
                     SRLWWLLRWLGHDAVAVLDGGWAAWCRAGGALSRERPSAVPGDFQPHLRSWTVDVD"
BASE COUNT          579 a          933 c         1056 g          705 t
ORIGIN      
        1 ccggggcgtc ggggtagaat ccgacgccct tttctttgcc cttgccggtc cgtggtccga
       61 agcccggccg tgccgcccga atcgagtcac gcatgcaatc cctgtggatg atcgccgcca
      121 gtttcctgtt cgcctgcatg ggtgtttgcg tcaagctggc ggcgcagacc cattcggcgg
      181 ttgaaatcac cttctaccgc agcttcatct ccctgctgct gatgttcggg ctggttcgcc
      241 tgcgcggcgt ccggctggcg acgccgcact ggcgctggca gatcagccgc ggcctggtcg
      301 gctttgcggc gctgttcgcc tatttctacg cgatcacgct gctgccgctg gcgactgcgg
      361 tgacgctcaa ctacacctcg gcgatcttcc tcgcactcta tctggcgctg gccggctggc
      421 aggtgcggcg cggcatgctc ggtgcgctgg cgctcggcct ggccggtgtc atcctcttgc
      481 tcaagccgac cttccaggcc gagcaactgg tcggcggtct ggtcggcctc gcttccggcg
      541 tgatggccgg aatggcttac ttcagcgtgc gcgaactggg cgcccgcggc gaatcggaaa
      601 tccgcaccgt cttctacttc tcgctcgtcg ccagcgtctg tgccgctgtc tggctcgtct
      661 ttggcgagat ccacccggtc gacgccgaca gtggcctgct gctgttcggc gtcgccggct
      721 tcgcgacgct cgctcaactg gcgatgacgc gcgcctacgc gcgcggcaag accctgctct
      781 cggcggcgct ggcctacagc acggtgatct tctccagcct cgccggcatg ctgttctggg
      841 gcgaggtgct gccggcgtcg gcgtgggcgg cgattggcct gatcgtcctg tcgggcatcg
      901 tggcgacgca tttttcgcgt gccagcccgg tggagcagga ttaaacctgt cagtcaggta
      961 ttctcatgtc aattcaggta taaaagtgca tcggtatatc gcttcggcgg cactattacg
     1021 aagaaaggac agtgacgaaa tgaaggtttt tttgggcggt acgtgcaacg agtcaacgtg
     1081 gcgtcgtcgg atcattccgc tactcgaagc tgaattcttc aatcctgtag tggatgactg
     1141 gacgcctgat cgcatggccg aagaactgcg acagcgggaa gagtgcgatg tgtgtttgta
     1201 cgtcatcacg ccgaagatga ccggtgttta ttcgattgcg gaagtgatcg acgacagcaa
     1261 caagcgaccg gagaaaactg tgttcgtcag gctgcgtgat gacgacaacg agaagtttac
     1321 ggatgggcaa tggaagtcgc tgggggcggt tgcgcaaatg gttgagcgca acggaggccg
     1381 agcgtttgac tccgtgaaaa gcgcggcgac acacctctcg cattgctgcc gacctgaatg
     1441 cagcaaggag cagcaatgac tgttttgggg cgactggcac gtgtcgattt gcgcgaggtt
     1501 tggccgcacg aggcctataa cttcacgccg tggatggctc aatcctctaa tttggcggtt
     1561 ttatccgaag agttggggct cgatctggaa ctggtcggtc aggaggttgc cgtcgggccc
     1621 tatgccgcgg atattgtttg caaggatatt ttcagcggtg cccatgtcct gatcgaaaat
     1681 cagctggaaa aaacggatca cagccatctg ggacagatac tgacttacgc cgcggcgctg
     1741 gaggcgagaa gtattgtttg gatcgcttcg cgtttcactg atgaacaccg tgcggcgatc
     1801 gactggctca acgagatcac gcacgacgaa tggcagttct tcgggcttga gattgaattg
     1861 tggcgtatcg gtgattcgga gccggcgcca aaattcaaca tgatttgtcg gccaaacgac
     1921 tggagtcggg cggtgcgcga tgaggctgcc aaagccggga gcgcatcgcc gacgcaagcc
     1981 ttgcagttga aattctggtc ggcatttcgc gattacatga tgctcaaggg gctccgcgcg
     2041 ccgaagccgt cggcacagtc ttggcaatcg tttcgcgttg ggcggacggg gttctccatt
     2101 gagggggtga ttaagcgtaa cgaacgccag atgctggttc gtctttacat caattgcgac
     2161 gatctaccgc caaaggcggt tttcaggtac ttgcgggatc gtcgagacag cgttgaagcg
     2221 agtatggcat cggtgatgga atggaacgaa ttgccagata agaagggttg cgtgatcgca
     2281 gtcgttcgtt cgaatagtga cctcgacgac gaaacggcct ggcctcagtt acatgcctgg
     2341 ctggaaagca atgttgcccg gatggaaaaa gtctttcgtc cgctgatcgt ctctctttcc
     2401 gctgctgatc tggcttaaac tggatacagc cacaacaaca acggagcaaa ccatgatcgt
     2461 catcgaccac aaggcccacc gggtcaatgt ttcggtgttc ggcgaattca cgctggccga
     2521 ctatcgtgaa ttcgaggaag tcgtcaatta caagatcaag ttcgaagggc cggtcgacct
     2581 gttcttcgac ctgaccaaga tgggcacgtt gacgcttgac gtcgcctggg aggaagtcaa
     2641 gttctcgcgc gcccacgcga acgatttcaa tcgcgtcgcg gtaattaccg acagcgagtg
     2701 ggtgacctgg agcgcctggc tgtcgcagac cttcgtcaat gccgacgtgc aggtcttcga
     2761 cgatcccgct gcggccgatg aatggctgga aggcgaggct tgaacttcgg gccgctgatc
     2821 cggccggcgc agttgctggc gcagctcggc gagcccgact gctgcatcgt cgatgtccgc
     2881 catcaattgg ccgatgtcgg ttacggcgag cgggcctacg gcgaggcgca cctgcccggt
     2941 gctttcttcc tgcattgcga ccgcgacctg tcgggtccgc tgaacggccg caacgggcgc
     3001 catcccttgc ccgatcccaa agtgctggcc ggcaggctgg cggcgctcgg catcggccgg
     3061 cagacccggg tggtcgccta cgacgacggt aacggcatga tcgccagccg tctctggtgg
     3121 ctgttgcgct ggctcggcca cgatgccgtc gccgtgctcg acggcggctg ggcggcctgg
     3181 tgccgggccg gcggggcctt gagccgggag cggccgagcg ccgtgccggg cgacttccag
     3241 ccgcacttgc gctcgtggac agtcgatgtt gac
//
LOCUS       DAOSDX010000245         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10371_length_3266_cov_3.663345, whole genome shotgun sequence.
ACCESSION   DAOSDX010000245 DAOSDX010000000
VERSION     DAOSDX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_3266_cov_3.663345"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLN02_06515"
     CDS             <1..1108
                     /locus_tag="PLN02_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOI52014.1"
                     /translation="DELQQGKPLVQPWVDQRTVAAVLSEWTGIPDIDMVTDDIEATLQ
                     LRERLEKRVFGQTGGIVALSKALQVARAGIHDPQRPLGVFMLTGPTGTGKTETAIALS
                     ELLFGGSHNLIQFNMNEFQEAHTAATLKGAPPGYVGYGKGGVLTEAVRRKPYSVVLLD
                     EFDKAHRDVHEVFYQVFDRGWMEDGEGRRVSFRNCLILMTSNIGAEEIEVAALAEQPA
                     TYGQLVQLAQGSLQRHFAPALLARMQVLPFYPLGLSALQSIAEHQFERLADRLEEQGV
                     TLEIDTTAVEWVANTAAGHPHRGRAIEDLIRATVLPSLAQELLAAKRDGLPLGRVRVV
                     ADGGLSLELLDRESEPENLSEPNDADQQEVTSCA"
     gene            1099..>3266
                     /gene="tssI"
                     /locus_tag="PLN02_06520"
     CDS             1099..>3266
                     /gene="tssI"
                     /locus_tag="PLN02_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HOI52015.1"
                     /translation="MRLIEIRSPVLLDADSLALSFTAKEEMSWTPSYELEIICKRPDL
                     DLDGLLGDTVAIDISLDKGNDIRTFHAYVLEGVDTGQRDEQFTYKLKLGSWLSFLSQN
                     QNCRIFQDLSAPEIVEEVFRGYGFAKFELDLKRTYDKREYCVQFKESDLAFTSRLLED
                     EGIYYFIRHGQDEHTVVISDSQVFVDQSGIYGQLDFNPDGEEGRPILDRESIQRLQRS
                     RKVRPNNIVLRDFNYHVPTDVLQTNAQEEQATLSGVQLEHYDYAAGYGESQRGEFLAK
                     MRLLAWQAEARILNGKANAKGVTVGEAFSLQLHPDNARNRRYFILSTKYEYINDIDST
                     SKGESAVCTFTALPDDVEFHPLRRYKKPTVPGIQSATVVGPEGVEVHTDELSRIRVHF
                     HWDRYTTKEEDSSCWLRVSQAWAGKGWGVIAMPRVGQEVIITYVDGDLDRPLMTGIVY
                     NGDLPPPYDLPKDIRYTGLVSRSLSYGGPENASSITFDDLRGAERVMIHAERDYQQTV
                     ERNSATSIGQDRYMMVTRTITQTSTTQIVMQAALIDITGVRALFTGAQLNFTGVCASF
                     VGANIEAMGLNAQFIGADIKMIGSETAFIGNEIVATGIQTHMTGITLKLTGESIGVTG
                     ISAQMTGVRLDITLVWLRITGACIDLTGLWGRITGMSIDATGIALALTGMAVIAFGMH
                     LELKGVTIEATSLAIKATGVEARVTGLWAKITAVDMDIGLL"
BASE COUNT          648 a         1078 c          991 g          549 t
ORIGIN      
        1 cgacgaactg cagcagggca agccgctggt tcagccctgg gtcgatcagc gcacggtcgc
       61 cgccgttctc tccgaatgga cggggattcc cgacatcgac atggtgaccg acgacatcga
      121 ggccaccttg caactgcgcg agcgcctgga aaagcgcgtc ttcggccaga ccggcggcat
      181 cgtcgcgctg agcaaggcgc tgcaggtggc gcgcgccggc atccacgatc cgcagcggcc
      241 gctcggcgtc ttcatgctga ccgggccgac cggcaccggc aagaccgaaa cggcgattgc
      301 gctctcggaa ctgctgttcg gcggttcgca caacctgatc cagttcaaca tgaacgagtt
      361 ccaggaagcg cacaccgccg ccaccctgaa gggcgccccg ccgggatacg tcggctatgg
      421 caagggcggc gtgctgaccg aggcggtacg gcgcaagccc tacagcgtcg tgctgctcga
      481 tgaattcgac aaggcccacc gcgatgtcca cgaagtcttc taccaggtct tcgaccgcgg
      541 ttggatggaa gacggcgagg ggcgtcgggt cagcttccgc aactgcctga tcctgatgac
      601 cagcaacatc ggcgccgagg aaatcgaggt tgccgcgctg gccgaacaac cggccaccta
      661 cgggcaactg gtgcaactgg cgcagggcag cctgcagcgc catttcgcgc cggcgctgct
      721 ggcccggatg caggtgctgc cgttctaccc cctgggcctg tcggccttgc aatcgatcgc
      781 cgagcaccag ttcgaacgcc tggccgatcg tctggaagag cagggggtaa cgctggagat
      841 cgacactacc gccgtcgagt gggtggccaa taccgcagcg ggccacccgc atcgcgggcg
      901 cgccatcgaa gacctgatcc gcgcgaccgt gctgccgtcg ctcgctcagg agttgcttgc
      961 tgccaagcgg gacggcttgc cgctcggcag ggtaagggtg gttgccgacg gcggcctctc
     1021 cctcgaactg ctggatcgcg agagcgagcc ggaaaacctt tcagaaccca atgatgccga
     1081 ccaacaggag gttacgtcat gcgcttgatt gaaatcagga gtcccgtatt actggatgcc
     1141 gattcactgg cactcagttt caccgccaag gaagaaatgt catggacgcc cagctatgag
     1201 ctggaaatca tctgcaaacg tccggatctc gatctcgacg gattgctcgg cgataccgtc
     1261 gccatcgaca tctcgctcga taagggcaac gatatccgga ccttccatgc ctatgtgctg
     1321 gaaggtgtcg ataccggtca gcgcgacgag caattcacct acaagctgaa gctcggtagc
     1381 tggttgtctt tcctcagcca gaaccagaac tgccggatct tccaggacct gtcggcgccg
     1441 gaaatcgttg aagaagtgtt ccgcggttac ggtttcgcca agttcgaact cgatctcaag
     1501 cgcacctacg acaagcgcga atactgcgtt cagttcaagg agagcgatct cgccttcacc
     1561 agccgcctgc ttgaggatga aggcatctat tacttcatcc gtcacggcca ggacgaacac
     1621 accgtcgtca tcagcgactc gcaggtcttc gtcgaccagt cggggatcta cggtcagctc
     1681 gacttcaacc cggacggcga ggaagggcgg ccgattctcg accgcgaaag catccagcgc
     1741 ctgcaacgca gccgtaaggt gcggccgaac aacatcgtcc tgcgcgactt caactaccac
     1801 gtgccgaccg atgtcctgca gaccaatgcc caggaagagc aggccacgtt gtccggcgtt
     1861 cagctcgagc attacgacta tgccgccggt tacggcgaat cgcagcgcgg cgaattcctc
     1921 gccaagatgc gtctgctcgc ctggcaggcg gaggcccgca tcctcaacgg caaggccaac
     1981 gccaaggggg tgacggtggg cgaagccttc tcgctgcaac tgcacccgga caatgcccgt
     2041 aaccgtcgct atttcatcct cagcaccaag tacgaataca tcaacgacat cgacagcacc
     2101 tcgaagggcg aaagtgccgt ctgtactttc accgcgctgc ccgacgacgt cgagttccac
     2161 ccgctgcgcc gctacaagaa gccgaccgtg ccgggtatcc agagcgcgac ggtggtcggt
     2221 ccggagggcg tcgaggtgca taccgacgaa ctgagccgga ttcgcgtgca cttccactgg
     2281 gaccgctaca ccaccaagga agaggattcc tcgtgctggc tgcgcgtttc gcaggcctgg
     2341 gccggcaagg gctggggcgt catcgccatg ccgcgggtcg gccaggaagt catcatcacc
     2401 tacgtcgatg gcgacctcga ccgcccgctg atgaccggca tcgtctataa cggcgatctg
     2461 ccgccaccct acgacctgcc caaggacatc cgctacaccg gcctggtttc ccgctcgctc
     2521 agctacggcg ggccggagaa tgccagctcg atcaccttcg acgacctgcg cggcgccgaa
     2581 cgcgtgatga tccacgccga gcgcgattac cagcagacgg tcgagcgcaa ctcggcgacc
     2641 agcatcggcc aggaccgcta catgatggtg acgcgcacca tcacccagac ctcgaccacg
     2701 cagatcgtca tgcaggcggc gctgatcgac atcaccgggg tgcgcgcgct gttcaccggc
     2761 gcccagctca acttcaccgg cgtctgcgca tcgtttgtcg gcgccaacat cgaagcgatg
     2821 ggactcaacg cccagttcat cggtgccgac atcaagatga tcggtagcga gaccgccttc
     2881 atcggcaacg agatcgtcgc caccggcatc cagacccaca tgaccggcat cacgctcaag
     2941 ctgaccggcg aatcgatcgg cgtcaccggc atctcggcgc agatgactgg cgtgcggctc
     3001 gacatcaccc tggtctggct gcgcatcacc ggtgcctgta tcgacctcac cggcctctgg
     3061 ggccgcatca ccggcatgtc gatcgacgcc accggcatcg ccctggcgct caccggcatg
     3121 gcggtgatcg ccttcggcat gcacctggaa ctgaaggggg tgacgatcga agcgacttcg
     3181 ctggcgatca aggcgaccgg tgtcgaagcg cgggtgaccg gtctgtgggc caagatcacc
     3241 gcggtcgaca tggacatcgg cctgct
//
LOCUS       DAOSDX010000246         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10374_length_3264_cov_3.729511, whole genome shotgun sequence.
ACCESSION   DAOSDX010000246 DAOSDX010000000
VERSION     DAOSDX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10374_length_3264_cov_3.729511"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLN02_06525"
     CDS             complement(<1..835)
                     /locus_tag="PLN02_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOI52016.1"
                     /translation="MTQKLLFGIDRLLADPELRRPLAGRRLALLAHPASVTADLTHSL
                     DALAALPDLQLSAAFGPQHGIKGDKQDNMVESADFIDPQHCIPIFSLYGEVRRPTAAM
                     LNTFDVLLVDLQDLGCRIYTFITTLRYVLEAAAAHGKSVWVLDRPNPAGRPVEGFTLR
                     AGWESFVGAGAMPMRHGLTMGELGHWFIAQLKLDVDYQVVTMQGWQPHAAPGYGWPLG
                     ERTWINPSPNAPNLSMARAYAGTVLLEGTTLSEGRGTTRPLEFLFGAPDIDAAKVLKE
                     MQ"
     gene            complement(832..1368)
                     /locus_tag="PLN02_06530"
     CDS             complement(832..1368)
                     /locus_tag="PLN02_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HOI52017.1"
                     /translation="MPTINFQPLSCHASSTPPSGLVIAVAARRGAAGELLLDYRLHGV
                     AALALPPPAVPGPADGLWQQTCCEAFVAPTAGPAYREFNFAPSGQWAIYDFAGYRLRN
                     ERWQPPGAPRIACRIDSDALLLSAEIPATLLPNGAARLGLSVVAATPAGDKSYWALAH
                     AADQPDFHLAASFMLPLP"
     gene            1493..2857
                     /locus_tag="PLN02_06535"
     CDS             1493..2857
                     /locus_tag="PLN02_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOI52018.1"
                     /translation="MTPLRINLEQEEIPTHWYNVVADMPNPPAPPLGPDGKPVPPEAM
                     GAIFPDPILEQEMSAERWIPIPEEVRQIYAQWRPAPLCRALRLEQALGTPAKIFFKYE
                     GVSPAGSHKPNSAVPQAYFNKLAGTKKLTTETGAGQWGSSIAYAGQMFGLPVRVFMVN
                     VSYQQKPFRRSMMQTWGAEVFASPTNLTNAGRAALAADPDCQGSLGLAISEAVEEAAA
                     DPNTCYTLGSVLNHVLLHQTVIGLEAKKQFAKIGLYPDVIFGPCGGGSSFGGIAFPFL
                     ADKAAGDPRAKNLRCVAVEPTSCPTLTKGAYTYDYGDASGYTPIMKMYTLGHDFMPPG
                     IHAGGLRYHGASPLLSQLFHEKLLEAVAVPQVGTFEAGVQFARAEGIIPAPESCHGIR
                     AAIDEALRCKATGEAKTILFCLTGHGHFDMASYDKYFAGKLEDYEYPAEAIVESLKHL
                     PQVG"
     gene            2862..>3264
                     /locus_tag="PLN02_06540"
     CDS             2862..>3264
                     /locus_tag="PLN02_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52019.1"
                     /translation="MKFIVFLLVLGNLLFYAFSQGLIGPSDVGESVRITQQIKPESIR
                     IVARGEAPPLPQPAPALEAEVPEPEEVPAEKPALTAKEAAAKEAAAKKEAAAKEAAAK
                     KEAAAKEAAAAKEAAAKEAAAKEAAAKEAAAK"
BASE COUNT          560 a         1078 c         1147 g          479 t
ORIGIN      
        1 cctgcatttc cttgagtacc ttggcggcgt cgatgtccgg cgcgccgaac aggaattcca
       61 gcggccgcgt cgtgccgcgc ccttcgctca gggtcgtgcc ttcgagcagc acggtgccgg
      121 cgtaggcgcg cgccatcgac aggttgggcg cgttcgggct gggattgatc caggtccgct
      181 cgcccagcgg ccagccgtaa ccgggcgccg cgtggggctg ccagccttgc atggtgacga
      241 cctggtaatc gacgtcgagt ttcagttggg cgatgaacca gtggccgagt tcgcccatcg
      301 tcaggccgtg ccgcatcggc atcgcgccgg cgccgacgaa gctttcccag ccggcgcgca
      361 gggtgaagcc ttcgaccggc cggccggccg ggttcgggcg gtcgagcacc cagacgctct
      421 tgccgtgcgc ggcggcggct tcgagcacgt agcgcagcgt ggtgatgaag gtgtagatgc
      481 ggcagccgag gtcctgcagg tcgaccagca ggacgtcgaa agtgttcagc atcgccgccg
      541 tcggtcgccg cacctcgccg tacaggctga agatcgggat gcagtgctgc ggatcgatga
      601 aatccgccga ttcgaccatg ttgtcctgct tgtcgccctt gatcccgtgc tgcggaccga
      661 aggcggcgct gagctggagg tcgggcaggg cggcgagggc gtcgaggctg tgcgtcaggt
      721 cggcggtcac cgacgccggg tgggcgagca gggcgaggcg gcggccggcg agcgggcggc
      781 gcagctcggg gtcggcaagc aggcggtcga tgccgaacag gagtttttgc gtcatggcag
      841 gggcagcatg aagctggcgg cgagatggaa atcgggctgg tcggcggcgt gcgccagcgc
      901 ccagtagctc ttgtcgccag cgggcgtggc ggcgacgacg ctgaggccga ggcgggcggc
      961 gccgttcggc agcagcgtgg cggggatttc agcgctgagc aacagcgcgt cgctgtcgat
     1021 ccggcaggcg atgcgcggcg cgcccggcgg ctgccagcgc tcgttgcgca ggcgatagcc
     1081 ggcgaagtcg tagatcgccc actggccgga cggcgcgaag ttgaattcgc ggtaggccgg
     1141 gcctgcggtc ggggcgacga aggcttcgca gcaggtctgt tgccataagc cgtcagccgg
     1201 gccgggaacg gccggcggcg gcagcgcgag ggcagcgacg ccatgcagcc ggtagtcgag
     1261 tagcagctcg ccagcggctc cgcgccgggc cgcgacggcg atcaccaggc cggacggtgg
     1321 cgtgctgctg gcatgacagg acaggggctg gaagttgatg gtcggcacca gtttgcgggt
     1381 ggaattcagg cgctacttta ccccggatcg gacggccgcg cccttggtcc gggcggggtt
     1441 ttggagtaat ttaccggctt tgcggcgccg cccgcaggga gagagaaaaa acatgacacc
     1501 gttgcgcatc aacctcgaac aagaagaaat ccccacccac tggtacaacg tcgtcgccga
     1561 catgcccaac ccgccggcgc cgccgctcgg gccggacggc aagccggtgc cgccggaagc
     1621 gatgggggcg atcttccccg acccgattct cgaacaggaa atgagcgccg agcgctggat
     1681 cccgattccc gaggaagtgc gccagatcta cgcccaatgg cggccggcac cgctgtgtcg
     1741 ggcgctgcgc ctggagcagg cactaggtac gccggccaag atcttcttca agtacgaggg
     1801 cgtttcgccg gccggttcgc acaagccgaa ctccgccgtg ccgcaggcgt atttcaacaa
     1861 gctggccggc accaagaagc tgaccaccga gaccggcgcc ggccagtggg gttcgtcgat
     1921 cgcctacgcc ggccagatgt tcggcctgcc ggtgcgcgtc ttcatggtca acgtcagcta
     1981 ccagcagaag cctttccgcc gctcgatgat gcagacctgg ggcgccgagg ttttcgcctc
     2041 gccgaccaat ctgaccaacg ccgggcgcgc cgcgctggcc gccgacccag actgccaggg
     2101 ctcgctcggc ctggcgattt cggaagcggt cgaggaagcc gccgccgacc ccaacacctg
     2161 ctacaccctc ggttcggtgc tcaaccacgt gctgctgcac cagaccgtga tcggcctcga
     2221 agcgaagaag cagtttgcca agatcggcct ctacccggac gtgatcttcg ggccctgcgg
     2281 cggcggttcc agcttcggcg gcatcgcctt ccccttcctg gcggacaagg cggccggcga
     2341 cccgcgcgcc aagaacctgc gctgcgtcgc cgtcgagccg acctcctgcc cgacgctgac
     2401 caagggcgcc tacacctacg actacggcga cgcctccggc tacacgccga tcatgaagat
     2461 gtacaccctc ggccacgact tcatgccgcc cggtatccac gccggcggct tgcgttacca
     2521 cggcgcctcg ccgctcctct cgcagctctt ccacgagaaa ctgctggaag cggtggcggt
     2581 gccgcaggtc ggcaccttcg aggccggcgt ccagttcgcc cgcgccgaag gcatcatccc
     2641 ggcgccggag tcctgccacg gcatccgtgc cgcgatcgac gaagccctgc gctgcaaggc
     2701 gaccggcgaa gccaagacga tcctcttctg cctgaccggt cacggccact tcgacatggc
     2761 ctcctacgac aagtatttcg ccggcaagct ggaggattac gaatacccgg ccgaagccat
     2821 cgtcgaatcg ctgaagcacc tgccccaggt cggctgagat cgtgaagttc atcgtcttcc
     2881 tgctcgtcct cggcaatctg ctgttctacg ccttcagcca gggcctgatc ggtccgtccg
     2941 acgtcggtga aagcgtgcgc atcacgcagc agatcaagcc ggaaagcatc cgcatcgtcg
     3001 cccgcggcga ggcgccaccg ctgccgcagc cggctccggc gctggaagcc gaagtgccgg
     3061 agccggagga ggtgccagcg gaaaaaccgg cgctgacggc caaggaagcg gcagcgaagg
     3121 aagccgcggc gaagaaggaa gccgcggcca aagaggcggc agccaagaag gaagctgcgg
     3181 caaaggaagc ggcggcagcc aaggaggcag cagccaagga ggcagcagcc aaggaggcag
     3241 cagccaagga ggcagcagcc aagg
//
LOCUS       DAOSDX010000247         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10376_length_3264_cov_3.201309, whole genome shotgun sequence.
ACCESSION   DAOSDX010000247 DAOSDX010000000
VERSION     DAOSDX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10376_length_3264_cov_3.201309"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLN02_06545"
     CDS             complement(<1..1005)
                     /locus_tag="PLN02_06545"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI52020.1"
                     /translation="MKARIDRMHRNSLFLAVLAAFPPLAVAQVPADGDAKMLGSVTVN
                     AYQATKQTSGATKTETPLAETAQSVSVIAREEMDARGVRNLNDAMRYVAGVALESTGI
                     DNRVDDFSIRGFDAGSWGNNVTIDGMRAPQGSQWNRTMIDSWNLERIEVLKGPAAVMY
                     GQVAPGGMVNQVSKTPNRERERILRLGVDGHGQYNAAFDLGGGSDDDRHLFRLVGLVR
                     QGDTQIEHTEEDHSFIAPSYTFQVSDATRLTLLGLYQRDHGGSTYQFLPMAGTLVPTQ
                     YGRIKNNTFIGEPDWNTYNRTIWTAGYQFEHAFNERWKFSQSARLTDVQSLFRTVVTN
                     G"
     gene            1118..2083
                     /locus_tag="PLN02_06550"
     CDS             1118..2083
                     /locus_tag="PLN02_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOI52021.1"
                     /translation="MFAVKKTPSPVIDADYLFRRIGEGFDIPHKPARAELLAGVFFQA
                     GLRGGLGLQCTDILHLCNLNTAFALMPAGTKIIVKLEGNARVKLDGQLLDLDAGDGES
                     ARPVATVLTLTRAVRFTRSSTAGTRERMVVITLSPAWIDEAGLNGRLPARHLNQLAWQ
                     PSQRTLGAVEQLLRPGDAEDPLERLAMEGRCLDIVGDAFTQLLARDHTSDHGLAPTHY
                     RRICRLRRLLDSGAADHLDMNGIAQQMGCNATTLQQHFRQAFASTIFDYLRQRRLERA
                     ASALRRDGVSVALAAEIAGYASQANFSTAFRRHFGESPRHFRNRC"
     gene            2297..2620
                     /locus_tag="PLN02_06555"
     CDS             2297..2620
                     /locus_tag="PLN02_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOI52022.1"
                     /translation="MRIAAPYLRIAHQIPGRTRFKIDAAILDEPAVRAFGEEGLGDAL
                     GAIRGVREIRLNKLARSCTVAYDNAVIPDRAWHDLLAGANTPAAQALLGIIEDKYEEV
                     CHGQL"
     gene            2607..>3264
                     /locus_tag="PLN02_06560"
     CDS             2607..>3264
                     /locus_tag="PLN02_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOI52023.1"
                     /translation="MANYDEAILRSRTLQPAAPFPLLHQAIRIAMYDEYAARAYYRHV
                     IEAYGNLPPFSNIVAAEEQHVAALGRLCEHYGIPRPQDPFPLETVVAPTWRANLQQGI
                     AAEIANVQLYQHLLSIQPPADVQRVFLNLQSASLENHLPAFQRALQSAIARESYHAAQ
                     GVPASQAYVRHGPLTGIVESGLSLLTRQHGAFGIVGPLVRNAHPALLAGIVAGGAAVH
                     Y"
BASE COUNT          579 a         1150 c          960 g          575 t
ORIGIN      
        1 gccgttggtg acgacggtgc ggaacagcga ttggacgtcg gtcagacgtg cgctctggct
       61 gaatttccag cgctcgttga aggcgtgctc gaactggtag ccggcggtcc agatcgtccg
      121 gttgtaggta ttccagtccg gttcgccgat gaaggtgttg ttcttgatcc gcccgtactg
      181 ggtcggcacc agcgtgccgg ccatcggcag gaactggtag gtcgatccgc catgatcgcg
      241 ctgataaagg ccgagcaggg tcagccgcgt cgcgtcggaa acctggaagg tataactggg
      301 cgcgatgaag gaatggtctt cctcggtatg ctcgatctgg gtgtcgccct ggcgaaccag
      361 gccgaccagg cggaagagat gccgatcatc gtcggaaccg ccgccgagat cgaaggcggc
      421 gttgtactgg ccatgcccgt cgacgcccag gcgcagaatg cgctcgcgct cccgattcgg
      481 cgtcttgctg acctggttga ccatgccgcc cggcgcgacc tgaccgtaca tcaccgccgc
      541 cggccccttg agcacctcga tccgctcgag attccagcta tcgatcatgg tccggttcca
      601 ctggctgccc tgcggcgccc gcatgccatc gatggtgacg ttgttgcccc agctaccggc
      661 atcgaagccg cgaatgctga aatcatcgac gcgattgtcg atgccggtgc tttccagtgc
      721 cacaccggca acgtagcgca tcgcgtcatt gagattgcgg acgccgcgcg catccatttc
      781 ctccctggcg atcaccgaca ccgactgggc tgtctcggct agcggagttt cggtcttggt
      841 cgcaccggaa gtctgcttgg tggcctggta ggcattgacc gtcaccgagc cgagcatttt
      901 ggcatcgccg tcggctggca cctgggccac ggcaagcgga gggaaggccg ccaggacggc
      961 caggaacaag gagttgcgat gcatgcggtc aatgcgggct ttcatgggat tcctcgatac
     1021 gttcattgat attgctgttg cgacttatta ccatttgcaa caaatggccg tagtattgca
     1081 aatgatcgtc acccgaactt ttgcctgagc aagaaaaatg tttgctgtaa agaaaacccc
     1141 atccccggtg atcgacgccg actacctctt ccgacgcatc ggcgaaggct tcgacattcc
     1201 ccacaagcca gcgcgcgccg aactgctcgc tggcgtcttt ttccaggccg ggctgcgcgg
     1261 cggactaggc ctgcaatgca ccgacatcct ccatctctgc aacctgaaca cggcctttgc
     1321 tctgatgccc gccggcacga aaatcatcgt caagctggaa ggcaatgccc gggtcaagct
     1381 ggacggccag ctcctcgatc tcgacgccgg cgacggtgag tcggcccgcc ccgtggccac
     1441 cgtgctgacg ctgacacgag cggtccggtt cacgcgcagc agcaccgccg gaacccggga
     1501 gcgcatggtg gtgatcacct tgtcgcctgc ctggatcgac gaagccggcc tgaacgggcg
     1561 attgccggcc cgccacctga atcagctcgc ctggcaaccg tcgcaacgca cgctcggcgc
     1621 cgtcgagcaa ctgctgcgcc ccggcgacgc cgaagatccg ctcgagcgcc tggccatgga
     1681 aggccgctgc ctggacatcg tcggcgatgc gtttacgcaa ctcctggcgc gcgaccacac
     1741 cagcgatcac ggactcgcac cgacgcacta ccggcgcatt tgccgtctgc gtcgtctgct
     1801 cgacagcggc gccgccgacc acctcgacat gaacggaata gcccagcaga tgggctgcaa
     1861 cgccaccacc ttgcaacaac atttccgcca ggccttcgcc agcaccatct tcgactatct
     1921 acgtcagcgc cgcctggagc gggcagcgag cgccctgcgg cgcgatggtg tcagcgtcgc
     1981 gctggccgcc gaaatcgctg gctacgcgag ccaggccaac ttctcgaccg cctttcggcg
     2041 ccatttcggc gaatcgccca gacatttccg caaccgctgc tgacacccgc accggcgccc
     2101 ggatccagca ctgcagccct tgatttatgt caagctgctg gagcgcagac ctgctaaggt
     2161 gcaaccgagg catccctctc accagccagc cagccgcccc gcggtagcca gccaatcgtt
     2221 tccacaagag aaatccatgg ctggaaattt tgctggcacc cccattccat catcgtccct
     2281 gccgcccgtc gaggccgtgc ggatcgctgc gccttacctg cgtatcgccc atcagattcc
     2341 cggtcgcacc cgcttcaaga tcgacgcggc gatcctcgac gagcctgccg tccgcgcctt
     2401 cggcgaggaa ggactgggcg acgcgctggg agcgattcgc ggcgtgcgcg agatccgtct
     2461 gaacaagctg gcgcgctcct gcaccgtcgc atacgacaac gcggtgattc ccgaccgcgc
     2521 ctggcacgac ctgctggccg gcgccaacac cccggccgcg caggcgctgc tcggcatcat
     2581 cgaagacaag tacgaggagg tctgtcatgg ccaactatga cgaggcgatc ctgcgcagcc
     2641 gcaccctgca gccggcggcg cccttcccgc tgctgcacca ggcgatccgc atcgccatgt
     2701 acgacgaata cgcggcccgc gcctactatc ggcacgtcat cgaggcctac ggcaatctgc
     2761 cgcctttctc gaacatcgtc gccgccgagg aacaacacgt cgccgcactc ggccgactct
     2821 gcgaacatta cggcatcccc cgcccgcagg atccctttcc gctcgaaacc gtcgtcgccc
     2881 ccacctggcg cgccaatctc cagcaaggga tcgcggccga gatcgccaat gtgcagctct
     2941 accagcacct cctgtcgatc cagccgccgg ccgatgtcca acgggtcttc ctcaacctgc
     3001 agtcggcctc gctggaaaac catctaccgg ctttccagcg cgccctgcag agcgccatcg
     3061 cgcgcgagag ctaccacgcc gcgcaaggcg tcccggccag ccaggcctat gtccgccacg
     3121 gcccgttgac cggcatcgtc gaaagcggtc tctccctgct cacccgccag cacggtgcct
     3181 tcggcatcgt cggcccgctg gtgcgcaacg cccacccggc actgctggcg ggaatcgtcg
     3241 ctggcggcgc cgccgttcac tatc
//
LOCUS       DAOSDX010000248         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10384_length_3262_cov_3.260368, whole genome shotgun sequence.
ACCESSION   DAOSDX010000248 DAOSDX010000000
VERSION     DAOSDX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10384_length_3262_cov_3.260368"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="PLN02_06565"
     CDS             <1..1882
                     /locus_tag="PLN02_06565"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOI52024.1"
                     /translation="EEKAIRLRYNKCLGSAVNPVLREGNSDRRAPAAVKNYAKKHPHS
                     MGEWKQWSQTHVSHMHGGDFYHGEKSLTLDKARRVRMELIAKGGKVSVLKPEVKLLEG
                     EIIDSMFMSKKALCDFYEQELEDCRQAGILFSLHVKATMMKVSHPIVFGHCVKIYYKE
                     AFEKHAKTFDELGINVNNGMVDLYEKIKQLPESKRDEIIRDLHACQEHRPRLAMVDSA
                     KGITNFHSPNDIIVDASMPAMMRAGGKMWGADGKQYDSKCVMPESTFARIYQEMINFM
                     KWHGNFDPRTMGTVPNVGLMAQQAEEYGSHDKTFEIAEDGIANIVDIDSGEVLLTQNV
                     EAGDIWRMCQVKDAPIRDWVKLAVTRARNSGMPAVFWLDPYRPHEAELIKKVNVYLKD
                     HDTSGLEIHVMSQVRAMRYTLERVARGLDTISVTGNILRDYLTDLFPIMELGTSAKML
                     SIVPLMNGGGMYETGAGGSAPKHVQQLTQENHLRWDSLGEFLALAVSLEELGIKEGNA
                     RAKLLAKTLDAATGKLLDNRKSPSPKTGELDNRGSQFYLAMYWAQELAAQKEDAELAA
                     HFAKLAKTLTDSEATIVAEFASVQGKPADIGGYYKADADKCKAVMRPSPTFNAALKTM
                     RA"
     gene            2085..>3262
                     /gene="icd"
                     /locus_tag="PLN02_06570"
     CDS             2085..>3262
                     /gene="icd"
                     /locus_tag="PLN02_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOI52025.1"
                     /translation="MTSHIQVPQGGAKIVPGQPVPVNPIIPFIEGDGIGIDITPVMIK
                     VIDAAVEKAYGGSRKIHWMEVYAGEKSTRIYGPDEWLPKETFDALKEYSVSIKGPMTT
                     PVGGGIRSLNVALRQELDLYQCVRPVRYFKGVPSPLKNPELVDMVIFRENTEDIYAGI
                     EWAAGSEGVKKVVDFLQQEMGVRKIRFPESSGIGIKPVSVEGTARLVKAAIQYAIDND
                     RKSVTIVHKGNIMKFTEGLFRDTCYQVAKDDFGAEPIDGGPWCRVRNPHTGREITIKD
                     AIADAFLQQILLRPAEYDVIATTNLNGDYISDALAAQVGGIGIAPGANISDQYACFEA
                     THGTAPKYAGLDKVNPGSLILSAEMMLRHMGWKEAADLVIKGMEAAIGDQQVTYDFAR
                     "
BASE COUNT          698 a         1052 c          999 g          513 t
ORIGIN      
        1 cgaggaaaag gcgatccgcc tgcgctacaa caagtgcctc ggttccgccg tcaacccggt
       61 gctgcgcgaa ggcaactcgg accgccgcgc gccggccgcc gtcaagaact acgccaagaa
      121 gcacccgcat tcgatgggcg aatggaagca gtggtcgcag acccatgtct cgcacatgca
      181 cggtggcgac ttctaccacg gcgaaaagtc gctgaccctc gacaaggccc gccgcgtgcg
      241 catggaactg atcgccaagg gtggcaaggt cagcgtcctg aagccggaag tgaagctgct
      301 cgaaggcgag atcatcgact ccatgttcat gagcaagaag gcgctctgcg acttctacga
      361 gcaggagctg gaagactgcc gccaggccgg catcctgttc tcgctgcacg tcaaggcgac
      421 gatgatgaag gtctcgcacc ccatcgtctt cggccactgc gtcaagatct actacaagga
      481 agccttcgag aagcacgcca agaccttcga cgagctgggc atcaacgtca acaacggtat
      541 ggtcgacctc tacgagaaga tcaagcagtt gccagaatcg aagcgcgacg agatcatccg
      601 cgacctgcac gcctgccagg aacatcgtcc gcgtctggcc atggtcgatt cggccaaggg
      661 catcaccaat ttccattcgc cgaacgacat catcgtcgat gcgtcgatgc cggcgatgat
      721 gcgtgccggc ggcaagatgt ggggcgccga cggcaagcag tacgacagca agtgcgtgat
      781 gccggaatcg actttcgccc gcatctatca ggaaatgatc aatttcatga agtggcacgg
      841 caacttcgat ccgcgcacca tgggcaccgt gccgaacgtc ggcctgatgg cccagcaggc
      901 cgaagaatac ggttcgcacg acaagacctt tgaaatcgcc gaggacggca tcgccaacat
      961 cgtcgatatc gacagcggcg aagtcctgct gacgcagaac gtcgaagccg gcgacatctg
     1021 gcgcatgtgc caggtcaagg acgcgccgat ccgcgactgg gtcaagctcg ccgtcacccg
     1081 cgcccgcaac tccggcatgc cggccgtctt ctggctcgac ccctaccgtc cgcacgaagc
     1141 cgagctgatc aagaaggtca atgtctacct caaggatcac gacaccagcg gcctggaaat
     1201 ccacgtcatg agccaggtgc gcgccatgcg ttacacgctc gagcgcgtcg cccgcggcct
     1261 ggacacgatt tcggtcacgg gcaacatcct gcgcgactac ctgaccgacc tgttcccgat
     1321 catggaactg ggcacctcgg ccaagatgct gtcgatcgtc ccgctgatga acggcggcgg
     1381 catgtacgaa accggtgccg gcggttcggc accgaagcac gtccagcaac tgacgcagga
     1441 aaaccacctg cgctgggatt cgctcggtga gttcctggcc ctggccgtat cgctcgaaga
     1501 actcggcatc aaggaaggca acgcccgcgc caagctgctg gcgaagacgc tcgacgccgc
     1561 taccggcaag ctcctcgaca accgcaagtc gccgtcgccg aagaccggcg aactcgacaa
     1621 ccgcggttcg cagttctatc tcgccatgta ctgggcgcag gaactcgcgg cgcagaagga
     1681 agacgccgaa ctggccgcgc acttcgccaa gctggccaag acgctgaccg acagcgaagc
     1741 gactatcgtc gccgagttcg cctcggtgca gggaaaaccg gccgatatcg gcggctacta
     1801 caaggccgac gccgacaagt gcaaggcggt gatgcgtccg agcccgacct tcaatgctgc
     1861 gctcaagacg atgcgcgcct gatcgttccg acatcgtcgg gcaacaagcc ggcaaggcct
     1921 ccacttgggt ggaggccttt ttcgtcaacc ttgctgaagt ttgccaatag ctgccaaggc
     1981 tggcgatttg tttcataatt cgccgaccgg ccccagggag acatgcggcc gggccgcccg
     2041 gaggccgcgg cctccgtcgc ccgaataccc atagaggagt agccatgaca tctcacatcc
     2101 aggttccgca aggcggtgcg aaaatcgttc cggggcagcc ggtcccggtc aatccgatca
     2161 ttcccttcat cgaaggcgac ggcatcggca tcgacatcac gccggtgatg atcaaggtga
     2221 tcgacgccgc cgttgagaaa gcctacggcg gcagccgcaa gatccactgg atggaggttt
     2281 atgccggcga gaagtcgacg cgcatctacg gcccggacga gtggctgccc aaggaaacct
     2341 tcgatgccct gaaggaatac tcggtgtcga tcaaggggcc gatgacgacg ccggtcggcg
     2401 gcggcatccg ctcgctcaac gtcgccctgc gccaggaact cgacctttac cagtgcgtgc
     2461 ggccggtgcg ttacttcaag ggcgtgccca gcccgctgaa gaacccggag ctggtcgaca
     2521 tggtcatctt ccgcgagaac accgaggaca tctacgccgg catcgagtgg gccgccggct
     2581 cggaaggcgt caagaaggtc gtcgacttcc tgcagcagga aatgggcgtg cgcaagatcc
     2641 gcttcccgga aagctcgggc atcggcatca agccagtctc ggtcgaaggc acggcgcgcc
     2701 tggtcaaggc ggcgatccag tacgccatcg acaacgaccg caagtcggtg accatcgtgc
     2761 acaagggcaa catcatgaag ttcaccgagg gcctgttccg cgatacctgc taccaggtgg
     2821 cgaaggacga cttcggcgcc gagccgatcg atggtgggcc gtggtgccgc gtgcgcaatc
     2881 cgcataccgg gcgcgagatc acgatcaagg acgcgatcgc cgatgccttc ctgcagcaga
     2941 tcctgctgcg gccggcggaa tacgacgtga tcgccactac caacctgaac ggcgactaca
     3001 tttccgacgc gctggcggcg caggtcggcg gcatcggcat cgcgccgggc gccaacatct
     3061 cggaccagta cgcctgcttc gaggcgacgc acggcacggc gccgaagtac gccgggctgg
     3121 acaaggtcaa tcccggctcg ctgatcctct cggccgagat gatgctgcgc cacatgggct
     3181 ggaaggaagc cgccgacctc gtcatcaagg gcatggaagc ggcgatcggc gaccagcagg
     3241 tgacctacga tttcgcccgc ca
//
LOCUS       DAOSDX010000249         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10402_length_3256_cov_3.317713, whole genome shotgun sequence.
ACCESSION   DAOSDX010000249 DAOSDX010000000
VERSION     DAOSDX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10402_length_3256_cov_3.317713"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLN02_06575"
     CDS             <1..712
                     /locus_tag="PLN02_06575"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI52026.1"
                     /translation="QKLRQRAELALARARAGENFAELTAAFSDAPDALQGGDLGWRPL
                     DRLPVLYAEAAGKLQAGGVSELLRSSAGFHVVKLVERRGANQPASVQQTHARHILIRT
                     NEVMSEADARHRLETVRERIVNGMDFAEQARIFSQDGSAAKGGDLGWLNPGDTVPDFE
                     RAMDALKPGELSQVIKSPFGMHLIQVMERRERDVSSERQRSVARQAIRERKLDEAYQD
                     WLRQLRDRTYVENRLEQQ"
     gene            709..1701
                     /gene="pdxA"
                     /locus_tag="PLN02_06580"
     CDS             709..1701
                     /gene="pdxA"
                     /locus_tag="PLN02_06580"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOI52027.1"
                     /translation="MTAPVIAVTSGEPAGIGPELCLQLAGWSGAGRPVVLGDRDLLRQ
                     RAGLLGLDIVLRDYEPAVPAAAGTLDLIHLPLAAPAEAGVLDAANGRYVLALLDRALA
                     GCQSGEFAAMATAPVHKGVINESGVAFTGHTEYLAEKTGTPLVVMMLAGDTDRGPLRV
                     ALATTHLPLKDVPAAITGELLERTLRIVDADLRGKYGIAEPRILVAGLNPHAGEGGYL
                     GREEIDIIEPALARLCAEGLQLSGPHPADTMFTPPVLAGGDAVLAMYHDQGLTALKYA
                     TFGNGINVTLGLPIIRTSVDHGTALALAGSGQADPGSLFAAVSEAARMAQRKLQ"
     gene            1781..2551
                     /gene="rsmA"
                     /locus_tag="PLN02_06585"
     CDS             1781..2551
                     /gene="rsmA"
                     /locus_tag="PLN02_06585"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOI52028.1"
                     /translation="MMKHIPRRRFGQNFLIDQGIIGAIVSAIDPKRSDTVVEIGPGLG
                     AITVPLLERVDRLHVVEIDRDLIARLKKQHPVERLAIHEGDALAFDFASIGSDLRLVG
                     NLPYNISTPLLFHLASYGQQVRDMHFMLQKEVVERMVAEPGCADYGRLSVMLQYSFWL
                     EWLIDVPPESFDPPPKVDSAVVRLIPKPPEQLTAKNPERLSTLVQAAFAQRRKMLRNN
                     LKGILDDAGFAALGIAPTLRPEDLPVEDYVRIANFLAG"
     gene            2607..>3256
                     /locus_tag="PLN02_06590"
     CDS             2607..>3256
                     /locus_tag="PLN02_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI52029.1"
                     /translation="MSSANLFRLVLLAAIWGASFLFMRIAAPVLGAAWLVFCRVGLAA
                     LFLWGVALVLRRPLALRANWRAFLVLGLLNSALPFLLFAYAARLTPASLLAVLNATAP
                     IWAALIGALLARSWPEPRVLGGLALGIAGVALLAGVEVGALPAGGGLAVLAGIGAAVC
                     YGIASQYTRRIGNLEPHAGAFGNLAAATLYLAPLALLSPLPEQMPPPGVLAAVVALG"
BASE COUNT          479 a         1214 c         1031 g          532 t
ORIGIN      
        1 gcagaagttg cgtcagcgcg ccgaactggc gctggcccgg gcgcgcgccg gggaaaactt
       61 cgccgaactg acggccgcct tctccgatgc tccggatgcc ctgcagggcg gcgacctcgg
      121 ctggcggccg ctcgaccgcc tgccggtcct ttacgcggaa gccgccggca agctgcaagc
      181 tggcggcgtc agcgagttgt tgcgttcctc ggccggcttt cacgtcgtca agctggtcga
      241 acggcgcggt gccaaccagc cggcctcggt gcaacagacg cacgcccgtc acatcctgat
      301 ccggaccaac gaagtgatgt cggaagccga cgcccgccat cgcctggaaa ccgtgcgcga
      361 acgcatcgtc aatggcatgg acttcgccga gcaggcgcgc atcttttccc aggacggttc
      421 cgccgccaag ggcggcgacc tcggctggct caatccgggc gataccgtgc ccgatttcga
      481 gcgggcgatg gacgcgctga aacccggcga actcagtcag gtcatcaagt cgccgttcgg
      541 catgcacctg atccaggtca tggagcgccg cgaacgcgac gtatcttccg agcgccagcg
      601 ttcggtcgcc cgccaggcga tccgcgagcg caagctcgac gaggcctacc aggactggct
      661 gcgccagttg cgcgaccgta cctacgtcga aaaccgcctg gagcagcaat gaccgcaccg
      721 gtgatcgccg tcaccagcgg cgaaccggcc ggcatcggtc ccgaactctg cctgcagctc
      781 gccggctggt ccggcgccgg tcgtccggtc gtcctcggcg atcgggatct gctgcgccaa
      841 cgcgccggac tactcggcct cgacatcgtc ctgcgcgact atgaacccgc tgttccggcc
      901 gcagccggca cgctcgacct catccatctg ccgctcgccg cgccggccga ggccggcgtt
      961 ctcgacgccg ccaacggccg ctatgtgctg gccctgctcg atcgcgcgct ggccggctgc
     1021 cagtccggtg aattcgccgc gatggcgacg gcgcctgtgc acaagggcgt gatcaacgag
     1081 tccggcgtcg ccttcaccgg ccacaccgaa tacctcgccg aaaaaaccgg cacgccgctg
     1141 gtggtgatga tgctggctgg cgacaccgat cgcggcccgc tgcgcgtcgc gctggccacc
     1201 acgcacctgc cgctgaagga cgtgccggcg gcgatcaccg gcgagttgct ggagcgcacg
     1261 ctgcgtatcg tcgacgccga cctgcgcggc aagtacggca tcgccgaacc gcgcatcctg
     1321 gtcgccgggt tgaacccgca cgccggcgag ggtggctacc tcggccgcga ggaaatcgac
     1381 atcatcgagc cggcgctcgc ccggttgtgc gcggaaggcc tgcaactctc cggaccccac
     1441 cccgccgaca ccatgttcac cccgcccgtg ctcgccggcg gcgacgccgt gctggccatg
     1501 taccatgacc agggcctgac cgcgctcaag tacgccacct tcggcaacgg catcaacgtc
     1561 acgctcggcc tgccgatcat ccgcacctcg gtcgaccacg gcaccgcatt ggccctggcc
     1621 ggttccggcc aggccgaccc cggcagcctg ttcgccgcgg tcagcgaagc ggcgcgcatg
     1681 gcacaaagga agctgcaatg aagggacacg tcgcccgcaa acggtttagt tccggcataa
     1741 cccgcctgcg gcgcgttagc cttctctgaa acggcgacca ctgatgaaac atattccacg
     1801 tcgccgtttt gggcagaact tcctgatcga ccagggcatc atcggcgcca tcgtttcggc
     1861 gatcgatccc aaacgcagcg ataccgtcgt cgagatcggc cccggcctcg gcgccatcac
     1921 cgtgccgctg ctcgaacgtg tcgaccggct gcacgtcgtc gagatcgacc gcgacctgat
     1981 cgcccgcctg aaaaagcagc atccggtcga acgccttgcc atccacgaag gcgacgcgct
     2041 tgccttcgac ttcgccagca tcggcagcga cctgcgcctg gtcggcaacc tgccctacaa
     2101 catctcgacg ccgctccttt tccacctcgc cagctacggc cagcaggtgc gcgacatgca
     2161 cttcatgctg cagaaggaag tcgtcgagcg catggtcgcc gaacccggct gcgccgacta
     2221 cggccggctg tcggtgatgc tgcagtacag cttctggctg gagtggctga tcgacgtgcc
     2281 gccggagagc ttcgacccgc cgcccaaggt cgattcggcg gtggtccggc tgatcccgaa
     2341 acccccggag cagctcaccg cgaagaatcc ggaacgcctg tcgacgctgg ttcaggccgc
     2401 tttcgcccag cgccgcaaga tgctgcgcaa caacctcaag ggcatcctcg acgacgccgg
     2461 tttcgccgcc ctgggcatcg ccccgacgct gcgtccggaa gacctgccgg tcgaggacta
     2521 cgtgcgcatc gccaacttcc tggccggctg aacggcggcg caagaatcgg attgcgccgc
     2581 ttgccggcgc tgccatactg cggcccatgt cctccgccaa cctgttccgc ctcgtcctgc
     2641 tcgccgcgat ctggggcgct tccttcctct tcatgcgtat cgccgcgccg gtgctcggcg
     2701 ccgcctggct ggtcttctgc cgcgtcgggc tggcggcgct cttcctgtgg ggcgtcgccc
     2761 tcgtcctgcg tcggccgctg gcgctgcgcg ccaactggcg cgccttcctg gtcctcggcc
     2821 tgctcaactc ggcgctgcct ttcctgctct tcgcctacgc tgcccgcctg acgccggctt
     2881 cgctgctcgc cgtgctcaac gcgacggcgc cgatctgggc ggcactgatc ggcgccctgc
     2941 tcgcccgtag ctggccggag ccgcgcgtgc tgggcggtct cgccctgggc attgccggcg
     3001 tcgcgctgct cgccggggtc gaagtcggcg ccctgccggc cggcggcgga ctggccgtcc
     3061 tggccgggat cggcgccgcc gtctgctacg gcatcgccag ccagtacacc cgccggatcg
     3121 gcaatctgga accccacgct ggcgccttcg gcaacctggc ggcggcaacc ctctacctgg
     3181 cgccgctggc gctgctttcg ccgttgcctg aacagatgcc gccacctggt gtgctggctg
     3241 cagtggttgc gctcgg
//
LOCUS       DAOSDX010000250         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10433_length_3251_cov_3.950250, whole genome shotgun sequence.
ACCESSION   DAOSDX010000250 DAOSDX010000000
VERSION     DAOSDX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10433_length_3251_cov_3.950250"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1661)
                     /locus_tag="PLN02_06595"
     CDS             complement(93..1661)
                     /locus_tag="PLN02_06595"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOI52030.1"
                     /translation="MIDSTVVDLSRLQFAATAFYHFLFVPLTLGLSILLAIMETVYVM
                     TGKEIYRDMTKFWGKLFGINFALGVTTGITMEFQFGTNWAYYSHYVGDIFGAPLAIEG
                     LMAFFLESTFIGLFFFGWDRLSKRGHLVINYLTGIASNFSALWILIANGWMQNPVGSE
                     FSFVTMRMEMTSFADLLLNPAAQAKFVHTVSAGYVTGSIFVLGISSWYLLRGVHVEFA
                     RRSFRIAASFGLAAACSVIVLGDESGYAVTEHQQTKLAALEAMWKTEPAPAPFTLFAL
                     PDDQQRTNHAEVQIPYALGLIATRSTDTVLPGIDQLEVRAAERIVTGIEAVKLLEKLR
                     AGSASETEKTRFAAIKDDLGYGLLLKKYTADVAQATPEMIARAARDTIPDVAPMFWTF
                     RIMVVLGFLMLALMLIAFWESAKNRIGEKRWLLRWALWSIPLPWLACEMGWFVAEYGR
                     QPWTIYGILPTNLSTSALSSASLYGSLAGFVGFYTLLLVAEMYLMVKFARLGPASLGT
                     GRYEGESALLNPAK"
     gene            2189..>3251
                     /locus_tag="PLN02_06600"
     CDS             2189..>3251
                     /locus_tag="PLN02_06600"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI52031.1"
                     /translation="MHRPKRLAVAIALLYSGIAAAQNPQTGAAQGEGNDTTGGTRILP
                     EIVVSAPPAESAGETHIDRRVIDALPRGNGDITSLLKIHPNVQFDSRAQQSNKGGEIA
                     PADISINGAKFYQNSYVLDGMGISNHLDPGIKTENMPHMLSGSAFGMNIDSSLVCDVV
                     VRDANVPVEYGGFTGGVVEAKTCEPKRAFGGQIAYQTTRSSWMHDYIDQRYKDAYRNS
                     NDSFLGQPKFTKQTWRLALEGKPTENTGLIGSIVQTTSEIPLAPYPVAGGVSPQSDTT
                     ETRKLQNFFVRGFWHPVGDIDLDASLTYAPGEDRRFINTWQNSGWSNHTGGKGANLGM
                     AWRLPAGTWTQRLSWTQVEG"
BASE COUNT          666 a         1034 c         1031 g          520 t
ORIGIN      
        1 ggccgtccat gatggcgaag ccgaccagca gcacgccgac cagcagccac cagacgagtt
       61 tcagggtttc gtaatcgagc atgacggttt cctcacttgg cgggattgag cagggcgctt
      121 tctccttcgt agcggccggt gccgaggctg gccggaccga ggcgggcgaa cttgaccatc
      181 aggtacatct cggcgaccag cagcagggtg tagaagccga cgaagccggc gagcgaaccg
      241 tagaggctgg cgctggatag cgccgaggtg gacaggttgg tcggcaggat gccgtagatg
      301 gtccacggct ggcggccgta ctcggcgacg aaccagccca tttcacaagc cagccagggc
      361 agcgggatcg accacagcgc ccagcgcagc agccagcgct tctcgccgat ccggttcttc
      421 gccgattccc agaaagcgat gagcatcagc gccagcatca ggaaaccgag gacgaccatg
      481 atgcggaagg tccagaacat cggcgccacg tcggggatgg tgtcgcgcgc ggcgcgggcg
      541 atcatctccg gcgtcgcctg ggcgacatcg gcggtgtatt tcttgagcag cagaccgtag
      601 ccgagatcgt ccttgatcgc cgcgaagcgg gtcttctccg tttcgctggc gctgccggcg
      661 cgcaattttt cgagcagctt gaccgcctcg ataccggtga cgatgcgttc ggcggcgcgc
      721 acttcgagct ggtcgatgcc gggcagcacg gtatcggtcg agcgcgtggc gatcaggccg
      781 agcgcgtagg gaatctgcac ttcggcatgg ttggtgcgct gctggtcatc gggcagcgcg
      841 aacaaggtga acggtgccgg cgccggctcg gtcttccaca tcgcttcgag cgccgccagc
      901 ttggtctgct ggtgctcggt caccgcgtag cccgactcgt cgccgaggac gatcaccgaa
      961 cacgccgcgg ccagcccgaa ggaagcggcg atgcggaagg aacggcgggc gaattcgaca
     1021 tgcacgccgc gcagcaggta ccaggaactg atgccgagga cgaagatcga gccggtgacg
     1081 tagccggccg acacggtatg gacgaacttc gcctgcgccg ccgggttgag cagcagatcg
     1141 gcaaacgagg tcatctccat gcgcatggtg acgaaactga actcggaacc gaccggattc
     1201 tgcatccagc cgttggcgat caggatccac agcgccgaga agttcgaggc gatgccggtc
     1261 aggtagttga tcaccaggtg gccgcgcttc gacaggcggt cccagccgaa gaagaagaga
     1321 ccgatgaagg tcgattcgag gaagaaggcc atcaagcctt cgatcgccag cggcgcgccg
     1381 aagatgtcgc cgacgtagtg cgagtaatag gcccagttgg tgccgaactg gaactccatg
     1441 gtgatgccgg tggtgacgcc gagcgcgaag ttgatgccga acagcttgcc ccagaacttg
     1501 gtcatgtccc ggtaaatctc cttgccggtc atgacataga cggtttccat gatggccagc
     1561 aggatcgaca gacccagcgt caagggaacg aagaggaaat ggtaaaaggc ggtggccgcg
     1621 aactgcagcc gtgacaggtc gacgactgtc gagtcgatca tgacgatact ccctggttga
     1681 tgagcagaca ggagtcgccg atgcccgatc cggcctgccg gcgccacggc aatgaatgcc
     1741 gcgcagcgcc cgcgatcgcc acggttccgt tcgtttgcat ctaccctccg gcctgtttcc
     1801 ggttatgtga ctggcgtccc ccgctgccgc cccgcgtgcc ttgctggctt gtctgtgatg
     1861 gcggacagca gaccggcgga ttctcggcgg gcaccaggca ctgcagaagt gaccgaatgt
     1921 cgcagcgggc aagcggccgc cggcaaggcc tcgcggcccg ctgcggcaat atgccgcacg
     1981 gggcttgagc tagcggccac tatcattcgc gagctatcga aaacgcagta tccgctgcca
     2041 acacccgccg cgagatggcg ctcccggcgt ggcaatgcgg agccgcgcaa tcgatcgccc
     2101 tggcgccgcg acggctacga aacgcactcc ccgtccggca caggccgcag caatcaacaa
     2161 ccaataaacg ataatttcag gggaaatcat gcaccgacca aaacggctgg cagttgccat
     2221 tgccttgctt tattcaggga tcgccgccgc gcagaatccg caaaccggcg ccgctcaggg
     2281 ggaaggaaac gatacgaccg gcggcaccag gatactgccg gagatcgtgg tctcggcgcc
     2341 gccggcggaa tccgccggcg agacgcatat cgaccgccgc gtcatcgatg ccctgccgcg
     2401 tggtaacggc gacatcacca gcctgctcaa gattcacccc aacgtgcagt tcgactcgcg
     2461 cgcccaacag tcgaacaagg gcggcgaaat tgctcccgcc gacatcagca tcaacggtgc
     2521 caagttctac cagaactcct atgttctcga cggcatgggc atcagcaacc atctcgaccc
     2581 cgggatcaaa accgaaaaca tgccacacat gctttcgggc agcgcctttg gcatgaacat
     2641 cgattccagc ctggtctgtg acgtggtcgt ccgcgacgcc aacgttccgg tcgaatacgg
     2701 cggtttcacc ggcggcgtcg tcgaagccaa gacctgcgaa ccgaagcggg ccttcggtgg
     2761 ccagatcgct taccagacga cacgctcgtc gtggatgcac gactacatcg accagcgtta
     2821 caaggatgcc taccggaact ccaacgacag ctttctcggg caaccgaaat tcaccaagca
     2881 gacctggcgc ctcgcactgg aaggcaagcc gaccgaaaac accggcctga tcggcagcat
     2941 cgtccagacc acttcggaaa tcccgctcgc gccctatccg gtcgccggcg gcgtcagccc
     3001 gcagagcgac acgacggaga cccgcaagct gcagaatttc ttcgtccgtg gcttctggca
     3061 cccggtcggc gacatcgatc tcgacgcttc gctgacctac gctcccggcg aagatcggcg
     3121 cttcatcaac acctggcaga attccggctg gagcaaccac accggcggca agggcgccaa
     3181 cctcggcatg gcctggcgac tgccggcggg aacctggacc cagcggctgt cgtggaccca
     3241 ggtcgaaggt t
//
LOCUS       DAOSDX010000251         3248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10447_length_3248_cov_3.776386, whole genome shotgun sequence.
ACCESSION   DAOSDX010000251 DAOSDX010000000
VERSION     DAOSDX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10447_length_3248_cov_3.776386"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PLN02_06605"
     CDS             complement(<1..1150)
                     /locus_tag="PLN02_06605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437590.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOI52032.1"
                     /translation="MKALLDLFKQVTAEEEFDAITIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRDRM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMVSNLQRGQL
                     LTEDQYLEMMEEHGDEFQALMGAEGIREMLRNLDLNIEVENLRAELDVTGSEAKNKKL
                     AKRLKILEGFQKSGIKPDWMILEVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLELKAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKG
                     KGGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIF"
     gene            complement(1171..>3248)
                     /gene="rpoB"
                     /locus_tag="PLN02_06610"
     CDS             complement(1171..>3248)
                     /gene="rpoB"
                     /locus_tag="PLN02_06610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOI52033.1"
                     /translation="SVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPLVGTGIE
                     RTVAVDSGTAVVALRGGVVDYVDAGRVVVRVNDDETVAGEVGVDIYNLTKYTRSNQNT
                     NINQRPMVKIGDVIAKGDVVADGASTDKGELALGQNMLIAFMPWNGYNFEDSILISER
                     VVAEDRYTSIHIEELTVVARDTKLGPEEITRDIASLGEVQLSRLDDAGIVYIGAEVQA
                     GDVLVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPAGIAGTVIDVQVFTRE
                     GIERDKRAQSIIDEHLRHYKLDLADQMRIVERDAFERIGRLILGKKANGGPKKLAKGT
                     VIDQAYLDGMDPHHWFDIRLADDEIALQLEQVKEGLEQARKDFDVAFEAKRKKLTQGD
                     ELPPGVQKMVKVYVAVKRRLQPGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVL
                     NPLGVPSRMNVGQILEVHLGLAAKGLGHKIGDMLRRQATAKEVRGFLEEVYNGNGKPE
                     DLDSLTDAEILEMAGNLKEGVPFATPVFDGAKEEEIKAMLALAGMPSSGQMTLFDGRT
                     GDAFERQVTVGYMHYLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEV
                     WALEAYGASYVLQEMLTVKSDDVNGRTKVYENIVKGEHKIDAGMPESFNVLVKEIRSL
                     AIDIDLERY"
BASE COUNT          578 a          992 c         1002 g          676 t
ORIGIN      
        1 ggaagatgaa gggcttgaac agttcgagcg ccatcagctt gggcaggccg cactgatgca
       61 gcttgagctg cggaccgacg acgatgaccg aacggccgga atagtcgacg cgcttgccga
      121 gcaggttctg ccggaagcgg ccgcccttgc ccttgatcat gtcggccagc gacttcagcg
      181 gacgcttgtt ggcgccggtc atcgccttgc cgcgacggcc gttgtcgagc aggctgtcga
      241 ccgcttcctg caacatgcgc ttttcgttgc ggacgatgat gtccggcgcc ttcagttcga
      301 gcaggcgctt caggcggttg ttgcggttga tgacgcgacg gtagaggtcg ttcagatcgg
      361 aggtcgcgaa acgaccgccg tccagcggca ccagcgggcg caaatccggc ggcagcaccg
      421 gcagtacttc gaggatcatc cagtccggct tgatgcccga cttctggaaa ccttcgagga
      481 tcttcaggcg cttcgcgagc ttcttgttct tggcttccga gccggtgacg tcgagttcgg
      541 cgcgcaggtt ctcgacttcg atgttgaggt cgaggttacg cagcatctcg cgaatgcctt
      601 cggcacccat cagcgcctgg aattcgtcgc catgctcttc catcatctcc agatactggt
      661 cttcggtcag caactgaccg cgctgcaggt tggagaccat gcccgggtcg gtgacgacat
      721 aggcttcgaa atagaggacg cgctcgatgt cgcgcagcgt catgtcgagg accatgccca
      781 gacgcgacgg cagcgacttg aggaaccaga tgtgggcgac cggcgaggcc agttcgatgt
      841 gacccatgcg gtcgcgacga accttggcca gcgtgacttc gacgccgcac ttctcgcaga
      901 tcacgccgcg atgcttgagg cgcttgtact tgccgcacag acactcataa tccttgatcg
      961 ggccaaagat cttggcgcag aacaggccgt cgcgttctgg cttgaaggtc cggtagttga
     1021 tggtttccgg cttcttgact tcaccgtagg accaggaacg gatcttctca ggcgaagcga
     1081 ggccaatggt aatcgcgtcg aattcttctt cggcggttac ctgcttgaac aggtcgagca
     1141 atgctttcat cgtttaactc ctgagcctaa tcagtaacgt tccagatcga tgtcgatcgc
     1201 cagcgaacgg atttccttga ccagcacgtt gaacgattcc ggcatgccgg cgtcgatctt
     1261 gtgctcgccc ttgacgatgt tttcgtacac cttggtacgg ccgttgacgt cgtcggactt
     1321 gacggtcagc atttcctgca gcacgtagga agcgccatag gcttccagtg cccacacttc
     1381 catttcaccg aagcgctgac caccgaactg cgccttaccg cccagcggct gctgggtgac
     1441 cagcgagtac gggccggtcg aacgggcgtg catcttgtcg tcaaccaagt ggtgcagctt
     1501 caggtagtgc atgtagccga ccgttacctg gcgctcgaag gcgtcaccgg tgcgaccgtc
     1561 gaacagggtc atctggcccg acgacggcat gccggccagc gccagcatgg ccttgatttc
     1621 ttcttccttg gcaccgtcga agaccggcgt cgcgaacggc acgccttcct tgaggttgcc
     1681 cgccatttcc aggatttcgg cgtcggtcag cgaatccaga tcttccggct tgccgttacc
     1741 gttatacacc tcctcgagga aaccacgcac ttccttggcc gtcgcctgac ggcgcaacat
     1801 gtcgccgatc ttgtggccga ggcccttggc ggcgagaccg aggtgaactt cgagaatctg
     1861 cccgacgttc atccgcgacg gcacgccgag cgggttcagc acgatgtcaa ccggacggcc
     1921 atcggccatg taaggcatgt cttcgaccgg caggatgcgc gagacaacac ccttgttacc
     1981 gtgacgaccg gccatcttgt cgccaggctg caggcgacgt ttgacggcga catagacctt
     2041 gaccatcttc tgcacgcccg gcggcagttc gtcgccctgc gtcagcttct tgcgcttggc
     2101 ttcgaaagca acgtcgaagt ccttgcgggc ctgttccaga ccttccttga cctgttcgag
     2161 ctgcagcgca atctcgtcgt cggccaggcg gatgtcgaac cagtggtgcg ggtccatgcc
     2221 gtccaggtag gcctggtcga tgaccgtgcc cttggccagc ttcttcggac cgccgttggc
     2281 cttcttgccg aggatcagac gaccgatccg ctcgaaggcg tcgcgttcga cgatacgcat
     2341 ctggtcggcg aggtcgagct tgtagtgacg caggtgctcg tcgatgatcg actgggcgcg
     2401 cttgtcgcgc tcgatgcctt cgcgggtgaa cacctggacg tcgatgacgg taccggcgat
     2461 gccggccggc acgcgcagcg aggtgtcctt cacgtcggaa gccttctcgc cgaagatcgc
     2521 gcgcagcagc ttctcttccg gcgtcagctg ggtttcgccc ttgggcgtga ccttgccgac
     2581 cagtacatcg ccggcttgga cttcggcgcc gatgtaaacg ataccggcgt cgtccagacg
     2641 ggaaagctgg acttcgccga gcgaagcgat gtcgcgggtg atttcctcgg gaccaagctt
     2701 ggtatcgcgg gcaacgaccg tcagttcctc gatgtgaatc gaagtgtagc ggtcttcggc
     2761 gacgacgcgc tcggagatca ggatcgagtc ttcgaagttg tagccgttcc acggcatgaa
     2821 ggcgatcagc atgttctgac cgagggcgag ctcgcccttg tcggtggagg caccgtcggc
     2881 gaccacatcg cccttggcga tgacgtcacc gatcttgacc atcggccgct gattgatgtt
     2941 cgtgttctgg ttggaacggg tgtatttcgt caggttgtag atatcgacgc caacttcacc
     3001 ggcaaccgtt tcgtcgtcgt tgacgcgcac cacaacacgg ccggcatcga cgtaatcaac
     3061 gacaccgcca cgcagggcaa caacggcggt acccgagtca acggcgacgg tccgctcgat
     3121 gccggtaccg accagcggct tttccggacg caggcaggga acggcctgac gctgcatgtt
     3181 ggcacccatc agcgcgcggt tcgcgtcatc gtgctcgagg aacggaatca gcgaggcggc
     3241 gacggaca
//
LOCUS       DAOSDX010000252         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10455_length_3245_cov_3.251724, whole genome shotgun sequence.
ACCESSION   DAOSDX010000252 DAOSDX010000000
VERSION     DAOSDX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10455_length_3245_cov_3.251724"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..269
                     /locus_tag="PLN02_06615"
     CDS             33..269
                     /locus_tag="PLN02_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HOI52034.1"
                     /translation="MREAFLATETKAACAFKDIGPGKWLADIYRLARLQLTALGVEHI
                     YGGDLCTVADERRFYSYRRDGAATGRMASLIWLA"
     gene            613..2337
                     /gene="phaC"
                     /locus_tag="PLN02_06620"
     CDS             613..2337
                     /gene="phaC"
                     /locus_tag="PLN02_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOI52035.1"
                     /translation="MAQGSDNNQAFLASAMQFLQAGQGYAQQFMEQFSKQAGIADQAP
                     ALDPQALTALQKEFMDRQMSLWQSVLAKQQGQAPTFVVRPEPGDRRFAAPEWHESPIY
                     DYLHQAYLLNVQYLKEMVEKLPADDAKARDKMRFIARQMADALAPTNFAATNPEFIKL
                     ALETKGQSITDGINNLIRDFEKGRISMTDESVFEVGRNIATTEGAVVFENEVMQLIQY
                     APLTAKVGSRPLLVVPPCINKFYIMDLQPDNSLIRYMVDQGNTVFLVSWRNPGEAQGH
                     LGWDDYLEQGPIQALSVVRELTKVKQVNALGFCVGGTILTSALAVLKARGDDCVASLT
                     LLTTLLDFSDTGEIGLFIDEQALTTREATIGQGGLLPARDLQNTFSFLRANDLVWNYV
                     QNNYLKGQKPQAFDLLYWNSDSTNLPGPFACWYMRHMYLDNSLREPGKLAMCGVKVDL
                     GQLAMPVYLLATREDHIVPWQSAYQSTRILGGQCRFVLGASGHIAGVINPASKNKRSY
                     WINDDVTTDAATWLAAAAEMKGSWWTDWSTWLQQHSGAQRAARKIGNTKYKAIEPAPG
                     RYVKERAV"
     gene            2366..3106
                     /gene="phbB"
                     /locus_tag="PLN02_06625"
     CDS             2366..3106
                     /gene="phbB"
                     /locus_tag="PLN02_06625"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HOI52036.1"
                     /translation="MLRVALITGGMGGLGEAICIKMAGLGYKVVTTHSPGNAKVDEWL
                     KNMNNMGYGFKAYPCDVTDFESAKACVETVTREVGPIDVLVNNAGITRDMTFKKMTKA
                     DWDAVIRTNLDSVFNMTKQVMEGMIERKWGRVINVASVNGQKGAFGQTNYSAAKAGMH
                     GFTKALALEVARQGVTVNTISPGYIGTKMVTAIPQDILDSKILPQIPVSRLGKPEEIA
                     GLVAYLASDEAAFVTGANISINGGQHMY"
     gene            3152..>3245
                     /locus_tag="PLN02_06630"
     CDS             3152..>3245
                     /locus_tag="PLN02_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI52037.1"
                     /translation="MTQRVALVTGAMGGLGTAICQQLAKAGHKVV"
BASE COUNT          688 a         1036 c          978 g          543 t
ORIGIN      
        1 tagggccggc tcatttcgaa gtcggcgacg aggtgcgtga ggctttcctc gccaccgaga
       61 ccaaggcggc ctgcgcattt aaagacattg gtccgggcaa atggctggcc gatatttacc
      121 ggctggctcg cttgcaattg acggcgctgg gtgtcgagca catctacggc ggcgatttgt
      181 gtacggttgc cgatgagcgg cgtttttatt cttatcgtcg cgacggcgcc gctaccggcc
      241 gcatggccag cctgatctgg ctggcttgac gcagccagcc gttgctgttc gcggaaggcg
      301 cgccgctcgc ggcgcacccg gctgccggcg aaatagagga tcagtccgca gggcagggcg
      361 ccgtagaaca ggaaagaaat gatcccggca acgaggctgg cctcgctggc ggcgaccagg
      421 acggtgacat agagccaccc gataacgatg atcatggcta gggacatccg ccgattctaa
      481 atcgaaagcg gccgcccggc agccggcaat cacgctgctg ctgaagcgcc gatttatgca
      541 ttgacagtgc cgggacgggt atggagaatc cagaagctga agcccacgac aaccaacgag
      601 aaacggcatt ccatggcgca aggatcggac aacaaccagg cattcctggc ttctgccatg
      661 cagttcctgc aggccggaca aggctacgcg cagcaattca tggaacagtt cagcaagcag
      721 gccggcatcg ccgaccaagc cccggcgctt gacccgcagg cgctgacggc gttgcagaag
      781 gagttcatgg atcgccagat gtccttgtgg caatccgtgc tcgccaagca gcagggccag
      841 gcgcccacct tcgtggtccg tccggaacca ggagaccgcc gtttcgcagc gccggaatgg
      901 cacgaaagcc cgatctacga ttacctgcac caggcctacc tgctcaacgt ccagtacctc
      961 aaggaaatgg tcgagaagtt gccggccgac gacgccaagg cgcgcgacaa gatgcgcttc
     1021 atcgcccgcc agatggcgga tgcgctggcg ccgaccaact tcgccgcgac caaccccgaa
     1081 ttcatcaagc tggcgctgga aaccaagggt cagagcatca ccgacggcat caacaacctg
     1141 atccgcgact tcgagaaggg gcgcatctcg atgaccgacg aatcggtctt cgaggtcggc
     1201 cgcaacattg ccacgaccga aggcgccgtc gtcttcgaga acgaggtgat gcaactgatc
     1261 cagtacgcgc cgctcaccgc caaggtgggc agccggccgc tgctggtggt gccgccgtgc
     1321 atcaacaagt tctacatcat ggacctgcag ccggacaatt cgctgatccg ctacatggtt
     1381 gaccagggca acaccgtctt ccttgtctcc tggcgcaatc cgggcgaagc gcagggtcac
     1441 ctgggctggg acgactacct cgaacagggg ccgatccagg cactgagcgt ggtgcgcgaa
     1501 ctgaccaaag tcaagcaggt caatgccctc ggtttctgcg tcggcggcac catcctgacc
     1561 tcggcgctgg ccgtgctgaa ggcgcgcggc gacgactgcg tcgcttcgct gaccctgctc
     1621 accaccttgc tcgatttttc cgacaccggc gagatcggcc tcttcataga cgaacaggcg
     1681 ctgaccacgc gcgaggcgac gatcggccag ggcggcctgc tgccggcgcg cgacctgcag
     1741 aacaccttct ccttcctgcg cgccaacgac ctggtctgga actacgtcca gaacaactac
     1801 ctcaagggcc agaagccgca agccttcgac ctgctctact ggaactcgga ttcgaccaac
     1861 ctgcccgggc cgttcgcttg ctggtacatg cgccacatgt acctcgacaa cagcctgcgc
     1921 gaacccggca agctcgccat gtgcggggtc aaggtcgatc tcggccaact ggcgatgccg
     1981 gtctacctgc tggcaacgcg cgaggaccac atcgtgccct ggcagtccgc ctatcagagc
     2041 acccgcattc tcggcggcca gtgccgcttc gttctggggg cctccgggca catcgccggg
     2101 gtgatcaacc cggcgagcaa gaacaagcgc agctattgga tcaacgacga cgtcacgacc
     2161 gacgccgcga cctggctggc cgcagcggcg gaaatgaagg gtagctggtg gaccgactgg
     2221 tcgacctggc tgcagcagca ctccggagca cagcgcgccg cgcgcaagat cggcaatacc
     2281 aagtacaaag ccatcgagcc ggcaccgggc cgttacgtca aggaacgtgc ggtctgattc
     2341 caataaaccc acggaggagc ggaaaatgtt gcgagttgca ctgattaccg gcggtatggg
     2401 tggcctcggc gaggccatct gcatcaagat ggcggggctg ggctacaagg tggtgaccac
     2461 ccattcgccc ggcaatgcca aggtcgacga gtggctgaag aacatgaaca acatgggcta
     2521 cggcttcaag gcctatccct gcgacgtcac cgacttcgaa tcggctaagg cctgcgtcga
     2581 aacggtgacc cgcgaggtcg ggccgatcga cgtcctggtt aacaacgccg gcatcacccg
     2641 cgacatgacc ttcaagaaga tgaccaaggc cgattgggat gccgtcatcc gcaccaacct
     2701 cgattccgtc ttcaacatga ccaagcaggt catggaaggc atgatcgaac gcaagtgggg
     2761 gcgcgtcatc aacgtcgcct cggtcaatgg ccagaagggt gccttcggcc agaccaacta
     2821 ctcggccgcc aaggccggca tgcacggttt caccaaggcg ctggcgctcg aagtcgcccg
     2881 ccagggtgtc acggtaaata ccatctcgcc cggctatatt ggcaccaaga tggtcaccgc
     2941 gatcccgcag gacatcctcg attccaagat cctgccgcag attccggtca gccgcctggg
     3001 caagcccgaa gaaatcgccg gcctcgttgc ctacctcgct tccgatgaag cggcgttcgt
     3061 gaccggggcc aacatctcca ttaatggcgg tcagcacatg tactgaccgg cgtttttcaa
     3121 ccccgcttca acaaaaaagg aaggaaacat catgacgcaa cgtgtcgctc tcgttaccgg
     3181 tgccatgggt ggtctcggta ccgccatctg ccaacaactg gccaaggccg gtcacaaggt
     3241 ggtcg
//
LOCUS       DAOSDX010000253         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10462_length_3243_cov_3.533250, whole genome shotgun sequence.
ACCESSION   DAOSDX010000253 DAOSDX010000000
VERSION     DAOSDX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10462_length_3243_cov_3.533250"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..477
                     /locus_tag="PLN02_06635"
     CDS             223..477
                     /locus_tag="PLN02_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HOI52038.1"
                     /translation="MDDQGKAYWSATLGLLGKILAIWFVCSFGAGILFADALNGIKLG
                     GYPLGFFFAHQGSIYIFIALIFYYAKAMGNLDRKFDVHED"
     gene            487..2343
                     /locus_tag="PLN02_06640"
     CDS             487..2343
                     /locus_tag="PLN02_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOI52039.1"
                     /translation="MDLQTMTYLVVGASFALYIGIAIWARAGSTSEFYAAGGSVHPIT
                     NGMATAADWMSAASFISMAGLIANMGYGGGLFLMGWTGGYVLLAMLLAPYLRKFGKFT
                     VPQFIGDRFYSQTASTVAVICLLTASITYIIGQMTGVGVAFSRFLGVSSDTGIYIGMG
                     IVFAYAVFGGMKGITYTQVAQYIVLILAYTVPAMFISLQLTGNPIRQLGLGSNISGTD
                     ISLLQKLDRVVTDLGFGQYTTSVPGSTLNMFVYTMSLMIGTAGLPHVIMRFFTVAKFS
                     DARSSAGWALVFIAILYTTAPAVGAMAKLNLHGTVNTAVGLQTAADGSMVVNADAVKA
                     NADVFATEASLVYEQRPEWMKRWEKTGLLKFEDKNGDGRIQYYNDKTKSADAKAKAEA
                     AGWKGNELTVNADIIVLANPEIALLPNWVIALVAAGGLAAALSTAAGLLMAISAAVSH
                     DLVKGVFMPNISEQAELRAGKISMAVAIVIAGYLGLNPPGFAAGTVALAFGIAASSLF
                     PAIMMGIFSKKMNKEGAIAGMLAGLFVTLFYVFAHKGIFFIKGTDFVDMIGGANSFFG
                     ITPEAFGAIGALVNFVVAFLVDKVTKEPPEHIQHMVENVRIPRGAKAVDGAH"
     gene            2490..2873
                     /locus_tag="PLN02_06645"
     CDS             2490..2873
                     /locus_tag="PLN02_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52040.1"
                     /translation="MTWILFLALFPMAFIWLRRAWRIAINKDFSEVALKHGESPANPE
                     KYAPYEAVINLVAGSVALAVIGGVLAGELAYDSWIAIAGSTIWCKIFLSFALSRQAHP
                     MNLPGFMQRRISLRPQPPAAERDDK"
     gene            complement(2883..>3243)
                     /locus_tag="PLN02_06650"
     CDS             complement(2883..>3243)
                     /locus_tag="PLN02_06650"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HOI52041.1"
                     /translation="ADETAAARRPVREVLLECDGVPVIFAESVLSTGRCGRLGRWFAR
                     LGSRSLGSLLFTHPGFGRGDIEFVRLRQRDALHRRVCQVLGRPLPPLWARRSRHHLDG
                     GSVLVSEVFLPTILDLA"
BASE COUNT          600 a         1077 c          948 g          618 t
ORIGIN      
        1 acaaaacaag aacaacaccc ctggccggcg ctctgccggc cacctgcaag gcccccaccc
       61 cggcgggggc cttttctttg ctcgatcgca aaacccataa cagtttcacc taatcgaaaa
      121 aaattgtcag ggaactgtaa gtttacaaac ccagactgtg tctcgtggcg atgttgcatt
      181 tcagtgcagc ccgcccatca cattttatgg aggagacaaa taatggacga tcaaggcaag
      241 gcctactggt cagccacact cggactgctg ggaaaaatcc tggcgatctg gttcgtgtgt
      301 tcattcggtg ccggcatcct ttttgccgac gcgctgaacg gcatcaaact cgggggttac
      361 ccgctgggct tcttctttgc ccaccagggt tcgatctaca tcttcattgc cctgatcttt
      421 tattacgcca aggcaatggg caacctcgac cgcaagttcg acgtgcacga agattaagga
      481 gccagcatgg atctgcaaac tatgacttac ctggtggtcg gcgcgtcgtt tgcgctgtac
      541 atcgggatcg cgatctgggc acgtgccggt agcacgtccg agttctacgc tgccggcggc
      601 agcgtccacc cgatcaccaa cggcatggca accgccgccg actggatgtc cgcagcctcg
      661 ttcatctcga tggccggcct gatcgccaac atgggttacg gcggcggcct gttcctgatg
      721 ggctggaccg gcggctacgt tctgctggcg atgctgctcg ccccgtacct ccggaagttc
      781 ggcaagttca cggtgccgca gttcatcggt gaccgcttct actcgcaaac cgcctcgacg
      841 gtggccgtga tctgtctgct caccgcgtcg atcacttaca tcatcggcca gatgaccggc
      901 gtcggcgtcg ccttctcgcg cttcctgggc gtttccagcg acaccggcat ctacatcggt
      961 atgggcatcg tcttcgccta cgccgtgttc ggcggcatga agggcatcac ctacacccag
     1021 gtcgcccagt acatcgtgct gatcctggcc tacaccgtcc ccgccatgtt catctcgctg
     1081 cagctgaccg gcaacccgat ccggcaactg ggcctgggct ccaacatctc cggcaccgac
     1141 atctcgctgc tgcagaagct cgaccgggtc gtcaccgacc tcggtttcgg ccaatacacg
     1201 acctcggttc cgggcagcac gctgaacatg ttcgtgtaca cgatgtcgct gatgatcggt
     1261 accgccggtc tgccgcacgt catcatgcgc ttcttcaccg tggccaagtt ttccgacgcc
     1321 cgttcgtcgg ccggctgggc gctggtattc atcgcgatcc tgtacaccac cgccccggcg
     1381 gtcggcgcga tggccaagct caacctgcac ggcaccgtca acaccgcggt cggcctgcag
     1441 accgctgccg atggctcgat ggtcgtcaat gccgacgcgg tcaaggccaa cgctgacgtg
     1501 ttcgccaccg aagcctcgct ggtctacgaa cagcgtccgg aatggatgaa gcgctgggaa
     1561 aagaccggtc tcctgaagtt cgaggacaag aacggcgacg gtcgcatcca gtactacaac
     1621 gacaagacga agagcgccga tgccaaggcc aaggccgaag ctgccggctg gaagggcaac
     1681 gagctgacgg tcaacgccga catcatcgtg ctggccaatc cggaaatcgc gctgctgccg
     1741 aactgggtta tcgccctggt tgctgccggt ggtctggctg ccgctctgtc gaccgctgcc
     1801 ggtctgctga tggcgatctc cgccgccgtg tcgcatgacc tggtgaaggg tgtgttcatg
     1861 ccgaacatct ccgagcaggc cgaactgcgc gccggcaaga tctcgatggc ggtcgccatc
     1921 gtcatcgccg gttacctcgg cctcaacccc ccgggcttcg ctgccggcac ggttgccctg
     1981 gccttcggta tcgccgcctc gtcgctgttc ccggcgatca tgatgggtat cttctcgaag
     2041 aagatgaaca aggaaggcgc catcgccggc atgctggctg gcctcttcgt caccctgttc
     2101 tacgtgttcg cgcacaaggg tatcttcttc atcaagggta ccgacttcgt cgatatgatc
     2161 ggcggtgcca acagcttctt cggcatcacg ccggaagcct tcggtgcgat cggtgcgctg
     2221 gtcaacttcg tcgtcgcctt cctggtcgac aaggtaacca aggaaccgcc ggagcacatc
     2281 cagcacatgg tcgaaaacgt ccgcatcccg cgtggcgcca aggctgtgga tggcgcgcac
     2341 taagcgcgat tgaggtaagc tcccgtccgg tgcccccgga cgggattccg aaccccgtcg
     2401 gtcgtttcca cggggttttt tattgcgcgg ctgccccggc gtccgcggct gtaccgaggt
     2461 gcaacatggc tttcaacgtc catatcgcaa tgacctggat cctgttcctg gcgctcttcc
     2521 cgatggcctt catctggctg cggcgcgcct ggcgcatcgc catcaacaag gatttttccg
     2581 aagtcgccct gaagcacggc gaatcaccgg ccaaccccga gaaatatgcg ccctacgaag
     2641 cggtgatcaa cctggtcgcc ggcagcgtcg cgctcgccgt gatcggcggc gtgctggccg
     2701 gcgaactggc ctacgacagc tggatcgcca tcgccggctc gaccatctgg tgcaagattt
     2761 tcctcagctt cgcgctgagc cgacaggcgc acccgatgaa cctgcccggc ttcatgcagc
     2821 gccgcatcag cctgcgcccg caaccgccgg ccgccgagcg cgacgacaag taatcggcaa
     2881 tttcaagcga ggtcgaggat cgtcggcagg aagacctcgc tcaccagtac gctgccgcca
     2941 tcgagatgat ggcgcgaacg acgcgcccag agcggcggca gcggccggcc aagcacctga
     3001 caaacccggc gatgcaaggc atcgcgctgg cgcaggcgga cgaattcgat atcgccgcgc
     3061 ccgaagccgg gatgggtaaa cagcagcgag ccgagcgaac ggctgcccag gcgggcaaac
     3121 cagcgcccga gacgaccgca acgccccgtc gacagcacac tttcggcgaa gatcaccggc
     3181 acgccgtcgc attcgagcag cacctcgcgt accggacgac gggccgctgc ggtttcgtcg
     3241 gcc
//
LOCUS       DAOSDX010000254         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10475_length_3241_cov_2.738858, whole genome shotgun sequence.
ACCESSION   DAOSDX010000254 DAOSDX010000000
VERSION     DAOSDX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10475_length_3241_cov_2.738858"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLN02_06655"
     CDS             complement(<1..973)
                     /locus_tag="PLN02_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52042.1"
                     /translation="MPAFPAILRSCAAILVFLAGMAGFGAQAANPPEASILFINSYHR
                     GYSWSDGIEDGFRERLVASDRKIELSVEYLDSRRFSYGAQIEPLVQAMAVKYADYQPD
                     VVVVADNAAFDFAIRYRDRLFPQRPIVFGGYNNFRPAVIEGIANITGVNEEVAIGDTV
                     NMALRVHPRTRTLAFIVSTGDASSKRIGEVAEETVFPELRKRFDVVVLKDASIEEIHQ
                     RLAKLPADSLLFLSGQVSDKSGSRTLSPAENGRRITAGSPFPAYTFWDFHLGAGALGG
                     HIIPPDEQGRTMAGIALRVLDGTPADSIPVVMTTPTRDVFDYPVMQRF"
     regulatory      1103..1309
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1411..>3241
                     /locus_tag="PLN02_06660"
     CDS             1411..>3241
                     /locus_tag="PLN02_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI52043.1"
                     /translation="MHIRLKPGAALLLPLFACSVQAATDLDTVVVTATRQESRIAEVL
                     ADVTVIDRAEIERNGQDTVTELLARQPGIQTASNGGPGTTTSFYLRGSNSNQTKVLVD
                     GIAINSADLSGSPLRFIPLADVERIEILRGPAATLYGADALGGVIHIITRRGAPGLRA
                     DGFVGYGSDNTREASAGVSGGDERWRFRVEANHRASDGFSAQRHASNHDADDDAYRNT
                     GGSAALSFIPAAGHEIGGTWRRNEGLIHYDSGNQPATGNYDDRARFTTEQWQLFSRNR
                     LSDAWTSRLQYGEAEDRQKSYYWDAWAWPVALEVVTRQNTRNRQLSWQNDITLPLGKL
                     LLAAERHEQEISPASGYASRPEVDNDSFLAGWTANLGDHSWQLNARHDDHSEFGGENT
                     WGVSYGYRLSRELRAHASYGTAFKAPSVYQLFMTSPWGNGNPDLKPEKSRNREIGLSW
                     DNGVHALSATWYHNRVSNLIDWVSDPVTWVGTYENVSKARLEGATLSYAGHFGVWNLR
                     ASYDWLDAKNTDNDFELGRRARNKAMLGVNRNWGPLETGVEVLGVGKRYNTNTETGRM
                     GGYGLVNLTARYAINSELAIEARLNNLFDKKYETVLGYNTPGMN"
BASE COUNT          635 a         1123 c         1003 g          480 t
ORIGIN      
        1 cgaagcgctg catcaccggg taatcgaaga catcccgggt cggcgtggtc atgaccaccg
       61 gaatgctgtc ggccggcgtt ccgtcgagaa cgcgcaaggc tatcccggcc atcgtccgtc
      121 cctgctcgtc gggcgggatg atgtggccgc ccagtgcacc ggcaccgaga tggaaatccc
      181 agaaagtgta ggccggaaac ggactgccgg cggtgatccg ccgcccgttc tcggccggcg
      241 acagggtgcg gctgccgctc ttgtcgctta cctgaccgct caggaaaagc aggctgtcgg
      301 ccggcagctt ggccagacgc tggtggattt cctcgatcga ggcatccttg aggacgacca
      361 catcgaagcg tttgcgcagt tcgggaaaca ccgtttcctc ggccacttca ccgatgcgct
      421 tgctgctggc atcgccggtg gaaacgatga aagccagggt gcgggtgcgc ggatgcacgc
      481 ggagcgccat attcacggtg tcgccaatgg caacttcctc attgacgccg gtgatgttgg
      541 cgatcccctc gatcaccgcc gggcggaaat tgttataccc gccgaaaacg ataggccgct
      601 gcggaaacag gcgatcgcga tagcggatgg cgaaatcgaa ggcggcattg tcggcgacca
      661 ccaccacatc gggctggtaa tcggcgtact tgaccgccat cgcctgtacc agcggttcga
      721 tctgggcgcc gtagctgaag cggcggctgt cgaggtactc gaccgagagt tcgattttcc
      781 tgtcggaagc gacaaggcgc tcgcgaaaac cgtcctcgat gccgtcgctc cacgaatagc
      841 cgcgatggta ggaattgatg aacaggatgc tcgcttccgg cggattcgcc gcctgcgcgc
      901 caaagcctgc cattcccgcg aggaagacga gaatggcggc gcacgaacgc aggatggcgg
      961 gaaaagctgg catgggctcg cggaatgatc gatcctgacg ggcatcatag ctcagtgccg
     1021 cgcggccatg cgcgccgcgc acagccacag tgcgtttcga ccagttgcat cgcccacgcg
     1081 ccgtcgcgcc tcccgctccg ctataattac gcccgtttca ggtgccgctc gcgaaattcc
     1141 tcgcgacggt gaaacgggaa agcggtgcgc ctccgacgag gcaattccgc tgctgccccc
     1201 gcaacggtaa gcgagtgcgg gcgcgccatc gagccactgg gttgatcccg ggaaggcggc
     1261 gcgtcaagac ttgcgagccc ggagaccggc ctgtgacgca agcggaagtg ccacggggtg
     1321 gtggcgccgg ctgattcagc ccatctcccc cgtgcacttt cttttcatca cacaccggca
     1381 tgcggggacg cctgccggca ggggaaaatc atgcacatcc gtctcaagcc cggcgcagcg
     1441 ctgctgttgc cgctattcgc ctgcagcgtc caggctgcca ccgatctcga caccgtcgtc
     1501 gtcaccgcca cccgccagga aagccgcatc gccgaagtgc tcgccgacgt cacggtgatc
     1561 gaccgcgcgg aaatcgaacg caacggccag gacaccgtga ccgagctgct cgcccggcaa
     1621 cccggcatcc agaccgcaag caacggcggc cccggcacca cgaccagctt ctacctccgc
     1681 ggcagcaact cgaaccagac caaggtgctg gtcgacggca tcgccatcaa ctcggccgac
     1741 ctctccggct cgccgctgcg tttcatcccg ctcgccgacg tcgagcgcat cgaaatcctg
     1801 cgcggcccgg cggccacgct ctatggcgcc gacgcgctcg gcggcgtgat ccacatcatc
     1861 acccggcgcg gcgcgccggg cctgcgcgcc gacggcttcg tcggctacgg cagcgacaac
     1921 acccgcgaag ccagtgccgg cgtcagcggc ggtgatgaac gctggcgctt ccgcgtcgag
     1981 gccaaccacc gcgccagcga cggtttttcg gcacagcgcc acgccagcaa ccacgatgcc
     2041 gacgacgacg cctaccgcaa caccggcggc agtgccgcgc tctccttcat tcccgccgcc
     2101 ggccacgaaa tcggcggcac ctggcggcgc aacgagggcc tcatccatta cgacagcggc
     2161 aatcagccgg cgaccggcaa ctacgacgac cgcgcccgtt tcaccaccga acaatggcag
     2221 ctcttcagcc gcaaccgcct gagcgatgcc tggaccagcc gtctgcagta cggcgaagcc
     2281 gaggaccggc agaagtcgta ttactgggat gcctgggcct ggccggtggc actcgaagtg
     2341 gtcacccggc agaacacgcg caaccggcaa ttgagctggc agaacgacat cacgctgccg
     2401 ctcggcaagc tgctgctggc ggccgaacgc cacgaacagg aaatcagccc ggccagcggc
     2461 tacgccagcc gcccggaagt cgacaacgac tccttccttg ccggctggac cgccaacctc
     2521 ggcgaccata gctggcagct caacgcgcgc cacgacgacc attccgaatt cggcggcgaa
     2581 aacacctggg gtgtctccta cggctaccgc ctgagccgcg aactgcgcgc ccacgccagt
     2641 tacggcaccg ccttcaaggc accgtcggtc taccagttgt tcatgacctc gccctggggc
     2701 aatggcaacc ccgacctcaa gccggagaaa tcgcgcaatc gcgagatcgg gctgagctgg
     2761 gacaacggcg tacacgcgct gagcgccacc tggtaccaca accgggtcag caacctgatc
     2821 gactgggttt ccgacccggt cacctgggtc ggcacttacg agaacgtcag caaggcccgc
     2881 ctcgaaggcg cgacgctgag ctacgccggc catttcggcg tctggaacct gcgcgccagc
     2941 tacgactggc tggatgcgaa gaacaccgac aacgatttcg aactgggccg gcgcgcgcgc
     3001 aacaaggcga tgctcggcgt caaccgcaac tggggaccgc tcgaaaccgg cgtcgaagtg
     3061 ctcggcgtcg gcaagcgcta caacaccaac accgaaaccg ggcgcatggg cggctacggc
     3121 ctggtcaacc tgacggcgcg ctacgcgatc aacagcgaac tggcgatcga ggcccgcctg
     3181 aacaacctgt tcgacaagaa gtacgaaacc gtgctcggct acaacacccc cggcatgaac
     3241 g
//
LOCUS       DAOSDX010000255         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10502_length_3235_cov_3.336164, whole genome shotgun sequence.
ACCESSION   DAOSDX010000255 DAOSDX010000000
VERSION     DAOSDX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10502_length_3235_cov_3.336164"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLN02_06665"
     CDS             complement(<1..494)
                     /locus_tag="PLN02_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOI52044.1"
                     /translation="MAITLPAGVQITAPIQPEWEFILSTEALEFVAKLHRAFEARRQE
                     LLKARIDRQARIDAGEMPDFLPETAHIRNGDWKVAPVPAALQCRRVEITGPVEAKMII
                     NAFNSGADSYMTDFEDSNSPNWENQIQGQVNLYKAIRRELSFKNENGKEYKLNDKIAT
                     LQIRP"
     gene            complement(533..1234)
                     /locus_tag="PLN02_06670"
     CDS             complement(533..1234)
                     /locus_tag="PLN02_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HOI52045.1"
                     /translation="MITPPTSASPKAIAFDAYATLFDVYCMEALAEEYFPCQGKALTL
                     LWRAKQVEYTRIRTMSGQFKTFWEVTRDALIFAARALELPMNDSQRQHLMNQYACLRP
                     FPENLATLEALKAMGFPLAILSNGTHSMLDIAVKYNDMNHLFAHILSADSVHKFKTAP
                     EVYQLAVDAFQLPKEEILFVSANAWDACAATWFGFTTFWVNRTNQPADVLDVTPHGSG
                     RELSDVLAWVGGLHS"
     gene            1317..2219
                     /locus_tag="PLN02_06675"
     CDS             1317..2219
                     /locus_tag="PLN02_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI52046.1"
                     /translation="MDTFKQISAFVNVATRGSLSAAARAEGVTPAIIGRRLDALESRL
                     GVKLLIRTTRKLTLTFEGQAFLEDCQKVLTDLANAEAAVSLGGVQASGYLKVSAPAGF
                     GRQHVAPLVGDFMRANPEVRVNLNLSDRLVDLINENIDCAIRIGELTDSSLVSVRLGE
                     MRRMVVASPAYLVEHGVPRSPDDLAGHNCLSLGQQRGWVFRDPADGNIDTLKVTGDFE
                     CNDGAVLHEWALAGRGLAWRSLWEVGRDLKEGRLTSVLDAWQAPPMGIYAVFPQRRHL
                     PLRVRLFIDLLKENYSRPSYWELR"
     gene            2222..2575
                     /locus_tag="PLN02_06680"
     CDS             2222..2575
                     /locus_tag="PLN02_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOI52047.1"
                     /translation="MRQPLYRALGVLSVLLGMIGVVLPLLPTTPFLILAAFFFARSHP
                     EWEARLLADPRVGPAILAWRERGAIPLLAKRLATLLLGISAVVGWFGLPGDWRYLPIS
                     CGALILLWMWSRPSA"
     gene            complement(2538..>3235)
                     /locus_tag="PLN02_06685"
     CDS             complement(2538..>3235)
                     /locus_tag="PLN02_06685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOI52048.1"
                     /translation="LPVIGMGLAGLILGGTAPDLAQYGGTLWFMLMMLGVAMFSGMSQ
                     CHYMATLVVRAMHDPLTGAYTRRSGEDALKLLFRLNHMAGKPLTLAFVDLDRFKEIND
                     LFGHEAGDQCLCLFADNLRAGLRRSDLLIRWGGEEFVAVLPDTPLDKVPLLLLRLRQQ
                     GLGQRPDGRQMTASFGIACSDENGIAACETLVWRADRRMYEAKRQGRDAVVWPDGSCQ
                     SLKPTDATTSTAG"
BASE COUNT          520 a          980 c         1120 g          615 t
ORIGIN      
        1 ggacggatct gcagggtggc gatcttgtcg ttgagcttgt attccttgcc gttctcgttc
       61 ttgaacgaga gttcgcggcg gatcgccttg tacaggttca cctggccctg gatctggttt
      121 tcccagttcg ggctgttgga atcctcgaag tcggtcatgt aggagtcagc gcccgagttg
      181 aaggcgttga tgatcatctt ggcctcgacc gggccggtga tctcgacgcg gcggcactgc
      241 agcgcggccg gcaccggcgc gaccttccag tcgccgttgc ggatgtgcgc ggtttccggc
      301 aggaagtccg gcatttcgcc ggcgtcgatg cgcgcctggc ggtcgatacg ggccttgagc
      361 agttcctggc ggcgggcttc gaaggcgcgg tgcagcttgg cgacgaattc gagcgcctcg
      421 gtgctgagga tgaattccca ttcgggctgg atcggggcgg tgatttgcac gccggcgggg
      481 agggtgatgg ccatgatgtc tcctgaagga tgggtgaagt tgagaaggaa attcagctat
      541 gcaggccgcc gacccaggcg aggacgtcgc tcagttcgcg gccgctgccg tgcggcgtga
      601 cgtcgaggac gtcggccggc tggttggtac gattgaccca gaaggtggtg aagccgaacc
      661 aggtggcggc gcaggcatcc caggcattgg ccgagacgaa gaggatttcc tccttcggca
      721 actggaaggc gtcgaccgcg agttggtaca cctcgggggc ggtcttgaat ttatgcacgc
      781 tgtcggcgga aaggatgtgc gcgaacaggt ggttcatgtc gttgtacttg acggcaatgt
      841 cgagcatcga atgcgtgccg ttggacagga tggcgagcgg gaaacccatc gccttcagcg
      901 cttccagcgt cgccaggttt tccgggaagg ggcgcaggca ggcgtactgg ttcatcaggt
      961 gctggcgctg gctgtcgttc atcggcagtt ccagcgcgcg ggcggcgaag atcagcgcgt
     1021 cgcgggtgac ttcccagaag gtcttgaact ggccgctcat cgtccggatg cgcgtgtatt
     1081 cgacctgctt ggcgcgccac aacagggtca gcgccttgcc ctggcagggg aaatactctt
     1141 cggccagcgc ctccatgcaa tagacgtcga acaaggtcgc gtaggcgtcg aaggcgatgg
     1201 ctttgggcga ggcggaggtt ggcggcgtga tcatggacat ggagtttatt gctaccaata
     1261 agataagata agcggcaaaa gaagcggtta ttcttttacc aaaagtaaat aatgcaatgg
     1321 atactttcaa gcagatttcg gcatttgtaa acgtcgcgac gcgcggcagc ctgtccgccg
     1381 ccgcccgcgc cgaaggcgtg acgccggcca tcatcggccg ccggctcgat gcgctggaaa
     1441 gccggctcgg cgtcaagctg ctgatccgca ccacgcgcaa gctgacgctg accttcgagg
     1501 gccaggcctt tctggaagat tgccagaagg tgctgaccga tcttgccaat gccgaggccg
     1561 cggtgtcgct gggcggcgtc caggcgagtg gttacctgaa ggtttcggcg ccggccggtt
     1621 tcggccgcca gcacgtggcg ccgctggtcg gcgacttcat gcgcgccaac ccggaagtgc
     1681 gggtcaatct caacctctcc gaccgcctgg tcgacctgat caacgagaac atcgactgcg
     1741 ccatccgcat cggcgaactg accgattcca gcctggtcag cgtccgcctg ggcgagatgc
     1801 ggcgtatggt ggtggccagc ccggcctacc tggtcgagca cggcgtgccg cgctcgccgg
     1861 acgatctcgc cggccacaac tgcctgtcgc tcggccagca gcgcggctgg gtgttccgcg
     1921 acccggccga cggcaacatc gacaccttga aggtgaccgg cgatttcgag tgcaacgacg
     1981 gcgccgtgtt gcacgaatgg gcgctggccg gtcgtggcct ggcctggcgc tcgctgtggg
     2041 aagtcggccg cgatctcaag gaaggccgcc tgacctcggt gctcgacgcc tggcaggcgc
     2101 cgccgatggg catctacgcg gtctttccgc agcgccgtca tctaccgttg cgggtccggc
     2161 tgttcatcga cctgctcaag gaaaactata gccggccgag ttactgggag ctgcgctgac
     2221 catgcgccag ccgctctatc gcgcgctcgg cgtgctgtcg gtgctgctcg ggatgatcgg
     2281 cgtcgtcctg ccgctgctgc cgacgacgcc tttcctgatc cttgccgcct tcttcttcgc
     2341 gcgttcgcat cccgaatggg aagcgcgact gctggccgat ccgcgggtcg gcccggcgat
     2401 cctcgcctgg cgcgagcgag gggcgatccc gttgctggcc aagcggctgg cgacgctgtt
     2461 gcttggcatc agcgccgtcg tcggctggtt cggcctgccc ggcgactggc gctatctgcc
     2521 gatcagttgc ggcgcgctca tcctgctgtg gatgtggtcg cgtccgtcgg cttgagcgac
     2581 tgacaactgc cgtccggcca gacgacggcg tcgcggccct ggcgcttggc ctcatacatg
     2641 cgccggtcag cccgccagac cagggtttcg caggcggcga tgccgttttc gtcgctgcag
     2701 gcaatgccga aagaggcggt catctgccgg ccgtccgggc gctgcccgag gccctgctgg
     2761 cgcaggcgca acagcagcag cggcaccttg tcgagcggcg tgtcgggcag gacggcgacg
     2821 aattcttcgc cgccccagcg gatcagcagg tcgctgcggc gcagtccggc gcgcaggttg
     2881 tcggcgaaca ggcacaggca ttggtcgccg gcttcgtggc cgaacaggtc gttgatttcc
     2941 ttgaatcgat cgaggtcgac gaaggccagg gtcaatggct tgccggccat gtggttgagc
     3001 cggaacagca gcttcaacgc atcttcgccg gagcgtcggg tatacgcgcc ggtcagcggg
     3061 tcgtgcatgg cgcgcacgac cagcgtcgcc atatagtgac actggctcat gccggagaac
     3121 atggcgacgc ccagcatcat caacatgaac cacagggtgc cgccgtattg cgcgaggtcg
     3181 ggcgcagtac cgccgaggat caggccggct agccccatgc cgatcaccgg cagcg
//
LOCUS       DAOSDX010000256         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10530_length_3228_cov_2.565396, whole genome shotgun sequence.
ACCESSION   DAOSDX010000256 DAOSDX010000000
VERSION     DAOSDX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10530_length_3228_cov_2.565396"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PLN02_06690"
     CDS             <1..1008
                     /locus_tag="PLN02_06690"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOI52049.1"
                     /translation="NADKSNAILVCHALSGHHHVAGHYADQPHNIGWWDNIIGPGRPL
                     DTDRFFVVGLNNPGGCHGSTGPSSINPATGKPWGADFPIVAVSDWVNAQARLADVFGI
                     DRWAAVMGGSLGGMQALRWTISYPERVRNALIIASAPKLTAQNIAFNDVARQAILTDP
                     DFHGGHFYEHGTRPVRGLRLARMLGHITYLSDDQMGEKFGRELRNGSFSFDYDVEFEV
                     ESYLRYQGDKFAGYFDANTYLLMTKALDYFDPARLDDGDLVRTMARTHRDTNFFVASF
                     TTDWRFSPARSREIVAALLANGRKVSYAEIDLNFGHDSFLMEDPQYHGLVAAFLGNIK
                     S"
     gene            1005..1616
                     /gene="metW"
                     /locus_tag="PLN02_06695"
     CDS             1005..1616
                     /gene="metW"
                     /locus_tag="PLN02_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOI52050.1"
                     /translation="MTHTLGRPDFDVIAAWVEPGHRVLDLGCGDGALLKHLIDTRNVH
                     GWGVDIEDANVLAAMRNGINVIQSNLENGLKDFADDAFDHVVLSRTLQTVRHTEGILR
                     EMLRVGREAVVSFPNFAYWRNLRSVLDGRMPVSEDLPYQWYDSPNVRFFTLLDFEALC
                     EQMGLIIVERRVLDEAGEIVQAETARDLNFLGSLAVYRLTRPH"
     gene            1652..2896
                     /locus_tag="PLN02_06700"
     CDS             1652..2896
                     /locus_tag="PLN02_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HOI52051.1"
                     /translation="MLICIFTGFASGLPLYLLLNLVPAWLKTEGLSLRTIGAFALIQF
                     PYTWKFLWAPLLDRYGILPWLGRRRSWMFVSQLGLVAAIGWLGQLSPRTDLMLIVGLT
                     ATLAFLSATQDIALDAFRREILPDEELGLGNAIHVNAYRIAGLVPGSLSLILADHLPW
                     SQVFMITAAFMIPGLLMTLAVGEPRAATGTPRTLRDAVVEPFREFFGRHGVQSALLVL
                     AFLFFYKLGDSLCTALATPFYLEMGYSKTEIGIIAKNAGLWPMVAGGILGGIWMVKLG
                     INRALWLFGVVQIVTILGFVWMAWIGPGESTPARLATLAIVIAGEAIGAGLGTTAFVA
                     FMARTTHPAYTATQLALFTSLMAVPRTFINASAGWLVEALGWNSFFWLCFLFAFPGMF
                     LLLRVAPWNESPAAQSAANPAG"
     gene            complement(2880..3224)
                     /locus_tag="PLN02_06705"
     CDS             complement(2880..3224)
                     /locus_tag="PLN02_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52052.1"
                     /translation="MAGLAEAAQSVALTVAERVAASSPGWFRQLLSSQRREARRVIVE
                     LMATRGLMPLLMKARNGDPWTAEERRILIEHLRRLAHLSPYLIGLLLPGSIVLLPIYA
                     WWLDRRRINRRD"
BASE COUNT          520 a         1176 c          950 g          582 t
ORIGIN      
        1 aacgctgaca aatccaacgc catcctggtc tgccatgcgc tttccggcca tcaccacgtt
       61 gccggccact acgcggacca gccacacaac atcggctggt gggacaacat catcggcccc
      121 ggccgcccgc tcgacaccga ccgcttcttc gtcgtcggct tgaacaatcc tggcggctgc
      181 cacggctcga ccggcccgtc gtcgatcaac ccggccaccg gcaagccctg gggcgccgat
      241 ttcccgattg tcgcggtcag tgactgggtg aatgcccagg cacgcctcgc cgacgtcttc
      301 ggcatcgacc gctgggcagc ggtgatgggc ggcagtctgg gcggcatgca ggcgctgcgg
      361 tggaccattt cctacccgga gcgggtacgc aacgcgctga tcatcgcctc ggcgccgaaa
      421 ctgaccgcgc agaacatcgc cttcaacgac gtcgcccgcc aggccatcct caccgacccc
      481 gattttcacg gcggccattt ctacgaacac ggcacgcggc cggtgcgcgg cctgcgcctg
      541 gcgcgcatgc tgggtcacat cacctacttg tccgacgacc agatgggcga gaaattcggc
      601 cgcgaactga ggaacggctc gttctccttc gactacgacg tcgaattcga ggtcgaatcc
      661 tacctgcgct accaaggcga caagtttgcc ggctacttcg acgccaacac ctatctgctg
      721 atgaccaagg cgctcgacta cttcgacccg gcccgcctcg acgacggcga tctggtcagg
      781 acgatggcgc gcacgcatcg cgacaccaat ttcttcgtcg cctcgttcac caccgactgg
      841 cgcttctcgc cggcccgctc ccgggaaatc gtcgcggcgc tgctggccaa cgggcgcaag
      901 gtttcctacg ccgaaatcga cctcaacttc ggccacgact ccttcctgat ggaagacccg
      961 caataccacg gcctggtcgc cgccttcctg gggaacatca agtcatgacc cataccctcg
     1021 gccgccccga cttcgacgtc atcgccgcct gggtcgaacc cggtcaccgg gtgctcgacc
     1081 tcggctgcgg cgacggcgcc ctgctcaagc acctgatcga cacccgcaac gtgcacggct
     1141 ggggcgtcga tatcgaagac gccaacgtgc tggcggcgat gcgcaacggc atcaacgtga
     1201 tccagagcaa cctggagaac ggcctcaagg atttcgccga cgacgccttc gaccatgtcg
     1261 tgctgtcgcg caccctgcag acagtgcgcc acaccgaggg catcctgcgg gaaatgctgc
     1321 gcgtcggccg cgaggcggtc gtctccttcc ccaacttcgc ctactggcgc aacctgcgtt
     1381 cggtcctcga cggccgcatg ccggtctcgg aagacctgcc ttaccagtgg tacgactcgc
     1441 ccaacgtgcg cttcttcacg ctgctcgatt tcgaggcact gtgcgagcag atggggctga
     1501 tcatcgtcga gcgccgcgtg ctcgacgaag ccggagagat cgtccaggcc gaaacggccc
     1561 gcgacctgaa cttcctcggc agcctcgccg tctaccgcct gacccgcccg cactgatgcc
     1621 cgcctggctg gccgctttgt ggaacaggcg gatgctgatc tgcatcttca ccggtttcgc
     1681 ctccggcttg ccgctctacc tgctgctcaa cctggtaccg gcctggttga agacggaggg
     1741 gctgtcgctg cgcactatcg gcgccttcgc gctgatccag tttccctaca cctggaagtt
     1801 cctctgggcg ccgctgctcg accgctacgg catcctgccc tggctgggcc ggcgccgtag
     1861 ctggatgttc gtctcgcagc tcggcctggt cgccgccatc ggctggctcg gccaactctc
     1921 gccgcgcacc gacctgatgt tgatcgtcgg cctgacggcg acgctggctt tcctgtccgc
     1981 cacccaggac atcgccctcg acgccttccg ccgcgaaatc ctgcctgacg aggagctcgg
     2041 cctgggcaac gcgatccacg tcaatgccta ccgcatcgcc ggcctggtgc cgggctcgct
     2101 gtcgctgatc ctcgccgacc acctgccgtg gagccaggtt ttcatgatca ccgccgcctt
     2161 catgatcccc ggcctgctga tgacgctggc cgtcggcgaa ccgcgcgccg ccaccggtac
     2221 accgcgcacg ctgcgcgacg ccgtggtcga gcccttccgc gaatttttcg gccgccatgg
     2281 cgtgcaatcg gcgttgctgg tgctcgcctt cctgttcttc tacaagctcg gcgactcgct
     2341 gtgcacggcg ctggccaccc ccttctatct ggagatgggc tacagcaaga ccgagatcgg
     2401 tatcatcgcc aagaacgccg gcctctggcc gatggtcgcc ggcggcatcc tgggcggcat
     2461 ctggatggtc aaactgggca tcaaccgtgc cctgtggctg ttcggcgtcg tccagatcgt
     2521 caccatcctc ggtttcgtct ggatggcctg gatcggcccc ggcgaatcga ctccggcccg
     2581 cctggcgacg ctggccatcg tcatcgccgg cgaagcgatc ggcgccggtc tgggcaccac
     2641 ggctttcgtc gccttcatgg caagaaccac ccacccggcc tacacggcca cccaactggc
     2701 cttgttcacc agcctgatgg ccgtgccgcg caccttcatc aacgccagcg ccggctggct
     2761 ggtcgaagcg ctgggctgga acagcttctt ctggctctgc ttcctgtttg ccttccccgg
     2821 catgttcctg ttgctgcgcg tcgccccctg gaacgagtcg ccagctgccc aatccgcagc
     2881 taatcccgcc ggttgatccg gcgccgatcc agccaccagg cgtagatcgg cagcaggaca
     2941 atggagcctg gcagcaaaag accgatcagg tagggcgaca ggtgcgccag acggcgcaga
     3001 tgttcgatca ggatgcggcg ttcctcggcc gtccaggggt cgccgttgcg cgccttcatc
     3061 aacaagggca tcaggccacg cgtcgccatc aattcgacga tcacgcgccg ggcttcgcgc
     3121 cgttgcgaag acagcagttg ccggaaccag ccgggactgg acgctgcgac acgctccgcc
     3181 acggtcagtg caacagactg cgcagcttct gccagccccg ccagacga
//
LOCUS       DAOSDX010000257         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10545_length_3224_cov_3.146418, whole genome shotgun sequence.
ACCESSION   DAOSDX010000257 DAOSDX010000000
VERSION     DAOSDX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10545_length_3224_cov_3.146418"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLN02_06710"
     CDS             <1..375
                     /locus_tag="PLN02_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOI52053.1"
                     /translation="GDILGLLTLEDIIEEIVGHFTTTTPGVDTDLTWNEDGSVVIDGT
                     RQIRDINRMLGLDFPLDGPKTLNGLILEHFQDIPEADVGLKLNDIAVEILQTRDRSIV
                     TARIYRPRPTEMPASLYKAPPP"
     gene            427..2142
                     /gene="pilB"
                     /locus_tag="PLN02_06715"
     CDS             427..2142
                     /gene="pilB"
                     /locus_tag="PLN02_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOI52054.1"
                     /translation="MAATPQNSPLSGLARALVQAGRLKEAEAEEVSRQATATKTSLIE
                     QLISSNKASAIEIARFSAETFGYPLFDLDALDERHIPQDAIDRKLIATHKVIPLNKRG
                     NRLSIAIADPTNLRALDEIRFQSGLSVDPIVVEQPKLLPLVAKYSESATDALKSFNDE
                     EINLDFLDEETPSKADEAAAQDIDDAPVVKFIQKILLDAINDGASDIHFEPYEKYYRI
                     RFRVDGMLREVATPPLAIKEKIASRIKVISRLNIAEKRVPQDGRMKLVISKSRAIDFR
                     VSTLPTLQGEKIVLRILDPTSATLGIEALGYEPDQKAILLDAINRPYGMVLVTGPTGS
                     GKTVSLYTCLNILNKDGVNIATAEDPAEINLPGINQVNVDDRAGLTFPVALKAFLRQD
                     PDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPTFNIASSI
                     LLITAQRLARRLCTCKQAIKVPEQALLDAGFKAEDLDGSWTLYGPGSCERCKGTGYKG
                     RVGIYQVMPISEAMQRLIMSGATSLDLAEQAKQEGVRDLRESGLLKVKQGLTSLEEVL
                     STTNI"
     gene            2159..>3224
                     /locus_tag="PLN02_06720"
     CDS             2159..>3224
                     /locus_tag="PLN02_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI52055.1"
                     /translation="MATAAKSRSVDVKESTFQWEGRNREGKTVRGEMRAATETVVQTT
                     LRRQGISSTKIKKIRFKSGGSISEKDITLFTRQLATMMKSGVPLLQTFDIVGRGHSNP
                     AVGKLLLDIKSEVETGSSLSQAFRKYPLYFDALYCNLVGAGEQAGILDSLLDRLATYK
                     EKILAIKGKIKKALFYPIAVLVVAFIITAVIMIFVIPAFKEVFSSFGADLPAPTLFVI
                     AISDFFVEWWWLIFGAIGGGLYAFFAAWKRSEKVQMIMDRFLLRLPIFGDIIRKAVIA
                     RWTRTLSTMFAAGVPLVESLESVGGASGNYIYKMATRQIQSDVSTGTSLTNAMQDATV
                     FPNMVTQMVAIGEESGALDSM"
BASE COUNT          760 a         1031 c          836 g          597 t
ORIGIN      
        1 ggcgacattc tcggtttgct cacactcgag gacatcatcg aagaaatcgt tggccacttc
       61 accaccacga caccgggggt agacaccgac ctgacctgga acgaggacgg cagtgtcgtg
      121 attgacggca cccggcaaat ccgcgacatc aaccgcatgc tcggactcga ttttccgctc
      181 gacggcccca agacactcaa cggtctgatc ctcgagcatt tccaggatat cccggaagcg
      241 gatgtcgggc tcaagctcaa tgacatcgcc gtggaaatcc tgcagacccg agatcgcagc
      301 atcgttaccg cccggattta ccggccgcgc ccgacagaaa tgccggccag tctttacaaa
      361 gcccctcccc cgtagcatca tcgggcgatc catacggcat ctaagcatcc gcacacaccc
      421 gatctgatgg cagcgactcc acaaaactcc ccgctcagcg gcctggcgcg tgcgctcgtc
      481 caggctggac gcctgaagga agccgaggcg gaagaagtgt cacgtcaggc cacagcgaca
      541 aagacctcgc tcatcgagca gttgatcagc agcaacaagg ccagcgccat cgagatcgcg
      601 cgcttttccg cagagacctt cggttatcca ttatttgatc tcgacgcgct ggacgagcgc
      661 cacatcccgc aggacgccat tgatcggaag ctgattgcaa cgcacaaggt cattcctctg
      721 aacaaaagag gaaaccgcct gtcgattgcc atagccgacc cgaccaacct gcgggctctt
      781 gacgaaatcc gtttccaaag cggcctgagc gttgacccca tcgtcgtcga gcaacccaag
      841 ctgctcccgc tggtcgcgaa gtactcggaa tccgccaccg acgcgctgaa gagcttcaat
      901 gacgaagaaa tcaaccttga tttcctcgac gaagaaaccc ccagcaaggc agacgaagcc
      961 gccgcccagg acatcgacga tgctccggta gtcaagttca tccagaagat tctgctcgac
     1021 gccatcaacg acggtgcttc ggacattcac ttcgagccct acgaaaaata ttaccggata
     1081 cgatttcgcg ttgacggcat gctccgcgag gttgccaccc cgccgctggc gatcaaggaa
     1141 aagatcgcct cgcggatcaa ggtcatctct cgcctcaaca tcgccgaaaa gcgcgtcccc
     1201 caagatggcc gaatgaagct ggtcatctcc aaatcacgcg ccattgactt ccgtgtcagt
     1261 accttgccca ctctgcaagg cgagaagatc gtgctgcgta ttcttgaccc gacctcggca
     1321 acgctgggaa tcgaagcgct gggctatgaa cccgaccaga aagccatcct gctcgacgcc
     1381 atcaaccgcc cctatggcat ggttctggtc accggcccaa ccggctccgg gaaaaccgtt
     1441 tccctctaca cctgcctcaa catcctcaac aaggatggcg tcaacatcgc cactgccgag
     1501 gatccggctg aaatcaacct ccccggaatc aaccaggtca acgtcgatga ccgcgccggc
     1561 ctgaccttcc ccgtagcgct gaaagcattc ctgcgccagg atcccgacat catcatggtg
     1621 ggtgaaatcc gcgacctgga aaccgctgaa atcgccatca aggcagccca gacaggccac
     1681 atggtgctat cgaccctgca caccaatgac gcaccgcaga ccctgacccg cctgatgaac
     1741 atgggggtgc cgaccttcaa catcgcctcc agcatcctgc tgatcacggc acaacgcctg
     1801 gcccgtcgcc tctgcacctg caaacaggca atcaaggttc cggaacaggc cttgcttgac
     1861 gcgggcttca aggccgaaga cctcgatggc agctggacac tatatggtcc aggcagttgc
     1921 gaacgctgca aaggcacggg ctacaaggga cgggtcggta tctaccaggt catgccgatc
     1981 agcgaagcca tgcaacgcct gatcatgagt ggtgccacgt cgcttgatct cgccgaacaa
     2041 gccaagcagg aaggggtccg cgatctacgc gaatcgggct tgctcaaggt caaacaaggc
     2101 ctgacttcgc tcgaagaagt tttaagtaca accaatatct aagctgaagg accaggcaat
     2161 ggcaactgca gcaaaatcaa gaagcgttga tgtcaaggaa agcacctttc agtgggaagg
     2221 tcgcaaccgg gaggggaaaa ccgtccgtgg cgaaatgcgc gccgcgaccg agacagtcgt
     2281 tcagaccacc ctgcgccgcc agggcatcag tagcaccaag atcaaaaaaa tacgcttcaa
     2341 atccggcggc agcatctccg aaaaggacat caccctattc acccggcagt tggccaccat
     2401 gatgaagtcc ggcgttcctt tgctgcagac tttcgacatc gttggccgcg gccatagcaa
     2461 tccagcagtc ggcaagttat tgctcgacat caagtccgaa gtcgaaaccg gaagttcgct
     2521 gtcacaggcc tttcgcaagt accccctcta tttcgacgcg ctttactgca acctggtcgg
     2581 cgccggcgaa caggcgggga ttctggactc tctgctcgac cgcctggcaa cctacaagga
     2641 aaaaatcctg gcgatcaagg gcaagatcaa gaaagccctg ttttacccga tcgccgtact
     2701 ggttgtcgcc ttcatcatca ccgccgtgat catgatcttt gtcattccgg cgttcaagga
     2761 ggtattcagc agtttcggcg ccgatctgcc ggccccgacc ctgttcgtca tcgccatctc
     2821 agacttcttc gtcgaatggt ggtggctgat ctttggcgcg ataggcggcg gcttgtacgc
     2881 tttctttgct gcctggaagc gttcagaaaa ggtacagatg atcatggacc gtttcctgct
     2941 gcgcctgccg attttcggcg acatcatccg caaggcggtg atcgcccgct ggaccagaac
     3001 cttgtcaacc atgttcgccg ccggcgtccc tctggtcgaa tcccttgaat cggtaggcgg
     3061 cgcttccggc aattacatct acaagatggc aacccgccag atccagagcg acgtatcgac
     3121 cggcaccagt ctgaccaacg cgatgcagga cgcgacagtc ttccccaaca tggtgacgca
     3181 gatggttgcc atcggcgaag aatccggcgc cctcgattcc atgc
//
LOCUS       DAOSDX010000258         3223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10548_length_3223_cov_2.952967, whole genome shotgun sequence.
ACCESSION   DAOSDX010000258 DAOSDX010000000
VERSION     DAOSDX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10548_length_3223_cov_2.952967"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLN02_06725"
     CDS             complement(<1..901)
                     /locus_tag="PLN02_06725"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOI52056.1"
                     /translation="MSIPLFKKLFARLSSPPPAKASAPKVDATPPAAAAPRLLFAWQE
                     LIGADRRIAGYRLLPRALDGDAAISGAQLSAGLAHENVRPAAEQRPLLIPLTAAQWAD
                     ADFTPLLAANSHLLITDLAACADPRALIDAIHVAGGRVAAYLPWQVDAQAELPVDLFL
                     LEFHEVPLAALEQRLQALARRRPGVGLLADGIESWDEFRLCQRLGITYCSGGFAASRD
                     QAASAERISESRLVVIDMLNQIRADAPLKAIAATAMRDPAVVVKLLEMANSPLYGLPR
                     KVAGIEEAITLLGRRQLQRWLQLL"
     gene            complement(968..1699)
                     /locus_tag="PLN02_06730"
     CDS             complement(968..1699)
                     /locus_tag="PLN02_06730"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HOI52057.1"
                     /translation="MSQPDDADLPFSLLQFYATSRYPCSYLPDREARSQVATPAHLID
                     SQIYGVLIRTGFRRSGLFTYRPHCDCCRACVPVRLPVAELRPTRSQRRAWKQHAGLQV
                     RELPLRFEPEHFDLYCRYQDSRHPGGGMDEDSREQYAQFLLQSHVDSRLIEFSDNGKV
                     RMVSVIDVLDDGLSSVYTFFDPDVPGASYGTFNILWQARQTQALGLPYLYLGYWIAES
                     RKMAYKTRFQPIEGYRDGNWVRLPA"
     gene            complement(1705..2406)
                     /gene="aat"
                     /locus_tag="PLN02_06735"
     CDS             complement(1705..2406)
                     /gene="aat"
                     /locus_tag="PLN02_06735"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOI52058.1"
                     /translation="MVPYLGPQMPFPPVATASAEMGGLLAVGGGLSPTRLLDAYRRGI
                     FPWGTVEGHPLWYSPDPRMVMFPEEFRLSRSLRRLLRSGPLRVSFDGDFRGVIAGCAA
                     TPRPGQDGTWISAEMMAAYVHLHELGWAHSVETWEDGKLVGGLYGLAIGRMFYGESMF
                     SRRDNASKFAFAHLIRHLRAHDFGMIDCQMYTPHLASLGAREIPRADFLSRLADLVGG
                     EFGSRWTAANLASVG"
     gene            complement(2412..>3223)
                     /locus_tag="PLN02_06740"
     CDS             complement(2412..>3223)
                     /locus_tag="PLN02_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI52059.1"
                     /translation="RRDEAASLRVRQMLATVGLAGLGDKYPHELSGGQQQRVALARAL
                     APRPELILLDEPFSNLDVGLRERLSFEVREILKREGSTAIMVTHDQNEAFAIADEIGV
                     MYHGRIQQWDTPYNLYHRPANRFVADFIGQGVLLPGVVGSGNSVRMELGTLLSDTPVE
                     CHESCAHCEDGCRVDVLLRPDDVVHDDASPVRAEVLHKAFRGAEILYTLRLASGSEVL
                     SLVPSHHNHALGEQIGIRLDADHVIAFKKHEACGDDPACERRLEQQAAAPA"
BASE COUNT          499 a          991 c         1226 g          507 t
ORIGIN      
        1 gcagcagttg cagccagcgc tgcaactggc gtcggccgag cagggtgatc gcctcctcga
       61 tcccggccac cttgcgcggc aggccgtaga gcggcgagtt ggccatctcc agcagcttga
      121 cgaccaccgc cgggtcgcgc atggcggtgg cggcgatcgc cttcagcggc gcgtcggcgc
      181 ggatctggtt gagcatgtcg atgaccacca gccggctttc gctgatccgc tcggcgctcg
      241 ccgcctgatc ccggctggcg gcgaagccgc cgctgcagta ggtgatgccg aggcgctggc
      301 agagccggaa ttcgtcccag gattcgatgc cgtcggcgag caacccgacc ccggggcgcc
      361 gccgggcgag cgcctgcagc cgctgttcga gtgcggccag cggcacttcg tggaattcga
      421 gcaggaacag gtcgaccggc agttcggcct gggcgtccac ctgccagggc aggtaggcgg
      481 cgacccggcc gccggcgacg tggatggcat cgatcagggc gcgcgggtcg gcacaggcgg
      541 caaggtcggt gatcagcagg tggctgttgg ctgcgagcag cggcgtgaag tcggcgtcgg
      601 cccattgggc ggcagtcagg gggatcagca gcgggcgctg ttcggcggcc gggcggacgt
      661 tttcgtgcgc caggccggcg ctcaactgag cgccgctgat cgccgcatcg ccgtcgaggg
      721 cgcgcggcag cagccggtag ccggcgatcc ggcggtcggc gccgatcagt tcctgccagg
      781 cgaaaaggag gcgcggcgcc gccgctgccg ggggcgttgc gtcgaccttg ggggcgctgg
      841 ctttggccgg cggcgggctg ctcagacgag cgaagagttt cttgaacaag gggatggaca
      901 aggtgggttg atctgcctgg catcataggc cagagcgcgg ctttcgggaa tgccggcagc
      961 cttgccctta cgccggcagg cgcacccagt tgccgtcgcg gtagccctcg atcggttgga
     1021 agcgcgtctt gtaggccatc tttcggcttt ccgcgatcca gtagccgaga tacaggtagg
     1081 gcaggccgag tgcctgggtc tgccgggcct gccagaggat gttgaaggtg ccgtagctgg
     1141 cgccggggac gtcggggtcg aagaaggtgt agaccgacga caggccgtcg tcgaggacgt
     1201 cgatgacgct gaccatgcgg accttgccgt tgtccgagaa ttcgatcagg cggctgtcga
     1261 cgtggctttg cagcaggaac tgggcgtact gttcgcggct gtcctcgtcc atgccgccgc
     1321 ccgggtggcg gctgtcctgg tagcggcagt agaggtcgaa atgctcgggc tcgaaacgca
     1381 gcggcagttc gcgcacctgc agcccggcat gctgcttcca ggcgcggcgc tggctgcgcg
     1441 tcgggcgcag ttcggcgacc ggcaggcgga ccggcacgca ggcgcggcag cagtcgcagt
     1501 gcggccggta ggtgaacagg ccgctgcgcc ggaaaccggt gcggatcagc acgccgtaga
     1561 tctggctgtc gatcaggtgg gccggcgtcg ccacctgcga gcgcgcctcg cgatccggca
     1621 ggtagctgca gggatagcgc gaagtggcgt agaactggag cagcgagaag ggcaggtcgg
     1681 cgtcgtcggg ctgggacatg gcggtcatcc aacgcttgcc aggttggccg ccgtccagcg
     1741 gctgccgaac tctccgccaa ccaggtcggc gagacgggac aggaagtcgg cgcgcgggat
     1801 ttcgcgggct cccagcgaag cgagatgggg cgtgtacatc tggcagtcga tcataccgaa
     1861 gtcatgagcg cggaggtggc ggatcaggtg ggcaaaggca aatttcgagg cgttgtcacg
     1921 gcgggaaaac atcgattcgc cgtagaacat gcggccgatg gccaggccgt agagtccgcc
     1981 gaccagcttg ccgtcttccc aggtttcgac cgaatgcgcc cagccgagtt catgcaggtg
     2041 gacataggcg gccatcatct cggcgctgat ccaggtgccg tcctggccgg ggcgcggagt
     2101 ggcggcgcag ccggcgatga cgccgcggaa atcgccatcg aaactgaccc gcagcggccc
     2161 gctgcgcagc agccggcgca gcgagcgcga caggcggaat tcctccggga acatcaccat
     2221 gcgcgggtcg gggctgtacc acagcgggtg accctcgacc gtgccccagg gaaagatgcc
     2281 gcgccgatag gcgtcgagca gccgtgtcgg cgagagtccg ccgccaacgg cgagcagccc
     2341 gcccatctcg gcactggccg tggcgaccgg cggaaaaggc atctgcgggc cgaggtaggg
     2401 gaccatccgg gtcaggccgg cgccgccgcc tgctgttcga ggcggcgttc gcaggcggga
     2461 tcgtcgccgc aggcttcgtg tttcttgaag gcgatgacgt ggtcggcgtc gaggcggatg
     2521 ccgatctgtt cgccgagcgc gtgattgtgg tgcgaaggaa ccagcgacag cacctcgctg
     2581 ccgctggcga ggcgcagggt gtacaggatt tcggcgccgc ggaaagcctt gtgcaggact
     2641 tcggcacgga ccgggctggc atcgtcgtgg accacgtcgt cggggcgcag caggacgtcg
     2701 acccggcagc cgtcctcgca gtgggcgcag ctttcgtggc attcgaccgg agtgtcggaa
     2761 agcagcgtgc ccagttccat gcgcacgctg ttgccgctgc cgacgacgcc cggcagcagc
     2821 acgccctggc cgatgaagtc ggcgacgaag cggttggccg ggcggtggta gaggttgtac
     2881 ggcgtatccc attgctggat gcggccgtgg tacatgacgc cgatctcgtc ggcaatggcg
     2941 aaggcttcgt tctggtcgtg ggtgaccatg atcgcggtcg aaccttcgcg cttgaggatt
     3001 tcccggactt cgaacgagag gcgttcgcgc aggccgacgt cgaggttcga gaagggttcg
     3061 tcgagcagga tcagttccgg tcgcggcgcc agcgcccggg ccagggcgac gcgttgttgc
     3121 tggccaccgg agagttcgtg cgggtatttg tcgccaagcc cggccaggcc gacggtggcc
     3181 agcatctggc gcacgcgcag gctggccgct tcgtcacggc gcc
//
LOCUS       DAOSDX010000259         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10561_length_3220_cov_3.163981, whole genome shotgun sequence.
ACCESSION   DAOSDX010000259 DAOSDX010000000
VERSION     DAOSDX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10561_length_3220_cov_3.163981"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /gene="aroA"
                     /locus_tag="PLN02_06745"
     CDS             complement(<1..1417)
                     /gene="aroA"
                     /locus_tag="PLN02_06745"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOI52060.1"
                     /translation="MEFLDLPQLLSAAGSVRLPGSKSISNRVLLLAALAEGETEVRDL
                     LASDDTERMLEALQTLGVGVMPLGGEAWKIAGCGGRFPVRQAELFLGNAGTAFRPLTA
                     ALALAGGDYVLKGVARMHERPIGDLVDGLRQLGADVTYLGNDGYPPLQLKPATIRPDG
                     AVKVRGDVSSQFLTGLLMALPLTGETVTVEVVGELISKPYIEITLATMARFGVVVERD
                     GWQRFTVRAGSCYVSPGTVYVEGDASSASYFLALGAIGGGPLRVEGVGADSIQGDVRF
                     AEALAQMGAIIETGPNWMAARAPQGGLVAVDLDCNHIPDAAMTLATAALFARGTTTLR
                     NIASWRVKETDRIAAMAIELRKLGAEVEEGADYIRVTPAPLKPAAIDTYDDHRMAMCF
                     SLAAFATPLRINDPKCVAKTFPDYFERFAAVTRAAPVIAIDGPSASGKGTVAARVAAA
                     LGFAYLDSGALYRLTALAAQRS"
     gene            complement(1486..2034)
                     /gene="rfbC"
                     /locus_tag="PLN02_06750"
     CDS             complement(1486..2034)
                     /gene="rfbC"
                     /locus_tag="PLN02_06750"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOI52061.1"
                     /translation="MQLVSTLLPGVLLLEPRVFGDERGFFFESFNQRAFDEVVGDHVD
                     FVQDNHSKSRRGVLRGLHYQLPPKAQGKLVRVVEGEVFDVAVDLRRASATYGHWAGAL
                     LSAANKRQMWIPPGFAHGFVTLSETAEFLYKTTDYYAPEYERCIRWNDPALAIDWGYS
                     REPQLSAKDAAAPGFAEAEKAF"
     gene            complement(2034..2921)
                     /gene="rfbA"
                     /locus_tag="PLN02_06755"
     CDS             complement(2034..2921)
                     /gene="rfbA"
                     /locus_tag="PLN02_06755"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOI52062.1"
                     /translation="MSQQRKGIILAGGSGTRLYPVTLAVSKQLLPIHDKPMIYYPLST
                     LMLAGIRDILVISTPQDTPRFQQLLGNGSQWGLNLQYAVQPSPDGLAQAFIIGRDFIG
                     GGDSALVLGDNIFYGHEFANDLAAAGAQPSGATVFAYHVHDPERYGVVEFDAAGRAVS
                     LEEKPKQPKSNYAVTGLYFYDNQVLDMARDLKPSARGELEITDINRLYLDRDQLNVQV
                     MGRGHAWLDTGTHESLLDASQFIATIEKRQGLKIACPEEIAFRKGWVDAAQLEKLAQP
                     MLKNPYGQYLIGLLGERRF"
     gene            complement(2918..>3220)
                     /locus_tag="PLN02_06760"
     CDS             complement(2918..>3220)
                     /locus_tag="PLN02_06760"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOI52063.1"
                     /translation="KTNLEVVHTLCDMLDELRPRADGQSYRTQITFVTDRPGHDRRYA
                     IDATKLERELGWKPAETFATGIRQTVQWYLDNPAWVQNVTSGAYKQWLGKHYGENA"
BASE COUNT          529 a         1031 c         1116 g          544 t
ORIGIN      
        1 tcgagcgctg cgcggcgagc gcggtcagcc ggtagagggc gccggaatcg aggtaggcaa
       61 aaccgagcgc ggcagcgacg cgcgccgcca ccgtcccctt gccggaagcc gacgggccgt
      121 cgatggcgat caccggcgcc gcccgggtga ctgcggcaaa gcgctcgaag taatcgggga
      181 aggtcttggc cacgcacttc ggatcgttga tccgcagcgg cgtcgcgaaa gcggcgagcg
      241 agaagcacat cgccatgcgg tggtcgtcgt aggtatcgat ggccgcaggc ttcagcggcg
      301 ccggggtgac gcggatgtaa tcggcgccct cctcgacctc ggcgccgagc ttcctcaact
      361 cgattgccat tgccgcgatg cggtcggttt ccttgacccg ccagctggcg atattgcgca
      421 gcgtcgttgt tccccgggca aacagcgcgg cggtagccag cgtcatcgcc gcgtcgggga
      481 tgtggttgca gtcgagatcg accgcgacca gccccccctg cggcgcgcgc gctgccatcc
      541 agttcgggcc ggtttcgatg atggcgccca tctgcgccag cgcttcggcg aagcggacat
      601 cgccctgaat cgagtcggcg ccgacgcctt cgacgcgtag cggcccgccg ccgatggcgc
      661 cgagcgccag gaaataggac gccgacgagg catcgccctc aacatacacc gtgcccggcg
      721 acacatagca gctgccggcc ctgaccgtga agcgctgcca gccgtcgcgc tcgacgacga
      781 cgccgaagcg ggccatggtg gccagcgtga tctcgatgta gggtttggag atcagctcgc
      841 cgacgacctc gaccgtgacc gtctcgccgg tcagcggcag ggccatcaag aggccggtca
      901 ggaactggct ggagacgtcg ccgcgcacct tcaccgcgcc gtccgggcgg atcgtcgccg
      961 gcttcagttg cagcggcggg tagccatcgt tgccgaggta agtgacatcg gcgccgagct
     1021 ggcgcaggcc gtcgaccagg tcgccgatcg gccgctcatg catgcgggcc acgcccttca
     1081 gcacgtaatc gccgccggcc agcgccagcg ccgcggtgag tgggcggaag gcagtgccgg
     1141 cgttaccaag aaagagttcc gcctggcgga ccgggaagcg accgccgcaa ccggcgatct
     1201 tccaggcttc tccgcccagc ggcatgacac cgacgccaag cgtctgcagg gcttccagca
     1261 tgcgctcggt gtcgtcagag gccaggaggt cgcgcacctc ggtctcgccc tcggcgagcg
     1321 ccgccagcag caggacgcgg ttggaaatgc tcttcgaacc gggcagacgg acgctgccgg
     1381 cggcggaaag cagttgggga agatcaagga attccatggc gaacccggcg gggtgaaaaa
     1441 tgcgccagtt tacccgggga ccgtcaaagt ccgaagtccc cgccctcaga aagccttttc
     1501 ggcctcggca aagcccggcg ccgcggcgtc cttggccgac agttgcggtt cccggctgta
     1561 gccccagtcg atggccagcg ccggatcgtt ccagcggatg cagcgctcgt actcgggcgc
     1621 atagtagtcg gtggtcttgt agaggaactc cgcggtttcg ctcagggtca cgaaaccgtg
     1681 ggcgaagccc ggcggtatcc acatctgccg cttgttggcg gcggacagca gggcgccggc
     1741 ccagtggccg taggtcgccg aggcgcggcg cagatcgacc gcgacgtcga acacctcgcc
     1801 ttcgacgacg cgtaccagct tgccctgggc tttcggaggc aactggtaat gcaggccgcg
     1861 cagcacgccg cgccggcttt tcgagtggtt gtcctgaacg aaatcgacgt gatcgccgac
     1921 cacttcgtcg aaagcgcgct gattgaaact ttcgaaaaag aaaccgcgct cgtcgccgaa
     1981 gacgcgcggt tcaagcagca gcacgccggg cagcaaggtg gagacgagct gcatcagaac
     2041 ctccgctcgc cgagcaggcc gatcaggtac tggccgtagg gattcttgag catcggctgc
     2101 gccagttttt ccagttgcgc cgcatcgacc cagcccttgc ggaaggcgat ttcctcggga
     2161 caggcgatct tcagcccctg gcgtttctcg atcgtggcga tgaactggct ggcgtcgagc
     2221 aggctttcgt gggtgccggt atcgagccag gcgtggccgc ggcccatgac ctggacgttg
     2281 agctggtcgc ggtcaaggta gaggcggttg atgtcggtga tttccagttc gccgcgcgcc
     2341 gacggcttga ggtcgcgcgc catgtcgagc acctggttgt cgtagaagta gaggccggtg
     2401 accgcgtaat tcgacttggg ctgtttcggc ttttcctcga ggctgaccgc ccggcccgcc
     2461 gcgtcgaatt cgacgacgcc gtaacgctcc ggatcatgca cgtgataggc gaagacggtc
     2521 gcccccgaag gctgtgcgcc ggccgcggcg aggtcgttgg cgaactcgtg gccgtagaag
     2581 atgttgtcgc cgagcaccag cgccgaatcg ccgccgccga tgaagtcgcg gccgatgatg
     2641 aaggcctgcg ccagaccgtc cgggctgggt tgcaccgcgt attgcaggtt cagcccccac
     2701 tggctgccgt tgccgagcag ttgctggaag cgcggcgtgt cctgcggcgt cgagatgacc
     2761 aggatgtcgc ggatgccggc gagcatcagc gtgctcagcg ggtagtagat catcggcttg
     2821 tcgtggatcg gcagcaactg cttggaaacc gccagcgtca ccgggtagag ccgggtaccg
     2881 gaaccgccgg cgaggatgat gcccttgcgc tgctgactca tgcgttttct ccgtaatgct
     2941 tgcccagcca ctgcttgtac gcgccgctgg tgacgttctg tacccaggcc gggttgtcca
     3001 ggtaccactg caccgtctgg cggatgccgg tggcgaaggt ttcggccggc ttccagccca
     3061 gttcgcgctc cagcttcgtc gcatcgattg cgtagcggcg gtcgtggccg gggcggtcgg
     3121 tgacgaaagt gatctgcgtc cggtaggact ggccatcggc gcgcgggcgc agttcgtcga
     3181 gcatgtcgca cagggtatgc acgacctcga ggttggtttt
//
LOCUS       DAOSDX010000260         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10595_length_3213_cov_2.676377, whole genome shotgun sequence.
ACCESSION   DAOSDX010000260 DAOSDX010000000
VERSION     DAOSDX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10595_length_3213_cov_2.676377"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PLN02_06765"
     CDS             <1..934
                     /locus_tag="PLN02_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HOI52064.1"
                     /translation="SYAIPLRLATYDGAAWEILLFAAPVLAWSRFSIAACPLPKSTLQ
                     TLTVQLGAHVFAAGNKLALADFLFSPDQLPRSFCDTWQLTRELGQAALAGENLTVDSA
                     HLPETNRFLADVRYLIGAIAVPRGGALFRWNEKDGAKEDALREWTRQGGPSLEPLFAG
                     CAWQPLLADAYHAACRNADRGSRPYSVKASIAFLQTTLGLMPDAIRAVIGPCYDRRME
                     EYRVGLGPKDGDAVYHGIVWPLLGGEDESTDCAGEIEAALREAGVKDVRFLDHHFPME
                     FCDDCGAPMFPNIDGELVHAEMPEQPSSSSQHLH"
     gene            934..2274
                     /gene="bioA"
                     /locus_tag="PLN02_06770"
     CDS             934..2274
                     /gene="bioA"
                     /locus_tag="PLN02_06770"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOI52065.1"
                     /translation="MSGNADWLARSQAAVWHPCTQMRHHADQASPAHLPLVPIARGAG
                     PWLYDFDGKRYLDGISSWWTNLFGHAEPRINAALREQLETLEHVMLAGFTHRPVVELS
                     ERLSDLTGGALGHAFYASDGASATEIALKMSFHYWRNVGRPEKAEFLCLAGSYHGETV
                     GALAVTDVAIFKDAYAPLVRHAAHVLPSPDFRQAEAGETPADVARRAAAGLADWLEKN
                     GARTAALIVEPLVQGAAGMAMSDPEYLRLARQLCDQHEVHLICDEIAVGCGRTGTFFA
                     HEQAEIRPDLICLSKGISGGYLPMSVVLCSDAIYAAFLDDSVARAFLHSHSYTGNPLA
                     CRAALATLDIFESDEVLATNRVKAQKIGAALAPLAAHPQVIHLRQRGMIAAFDVVTDD
                     PHFSRRFYRAALDAGALLRPIGNTVYLMPPYVVSDDEIALLGRVARQALEIALR"
     gene            2271..2618
                     /locus_tag="PLN02_06775"
     CDS             2271..2618
                     /locus_tag="PLN02_06775"
                     /inference="COORDINATES: protein motif:HMM:NF018628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HOI52066.1"
                     /translation="MTGLFLKSLLGAAAVLIIALLSRSKSFYIAGLVPLFPTFALIAH
                     AIVGVERGMTDLRTTALFGLWSLIPYAVYLLAVYWLSARLALAPTLISATLAWVVAAA
                     LLLFVWMRVYGSA"
     gene            complement(2882..3190)
                     /locus_tag="PLN02_06780"
     CDS             complement(2882..3190)
                     /locus_tag="PLN02_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HOI52067.1"
                     /translation="MAGIFEQLAERRISEALARGDLDHLPGAGKPLVFDDEPLLSPEQ
                     RMVNRILKNAGFTPREVLLRREIATLRAEVESLPAGDQRDEKRRELLLLLVQVAEGGA
                     "
BASE COUNT          480 a         1192 c         1038 g          503 t
ORIGIN      
        1 gagctacgcg atcccgctgc ggctcgccac ctatgacggc gcagcctggg agatcctgct
       61 cttcgcggcg ccggtattgg cctggtcgcg cttttctatc gccgcctgcc cgctgcccaa
      121 gagcacgctg cagacgctca ccgtgcagct cggcgcgcat gtcttcgccg ccggcaacaa
      181 gctggcgctg gccgatttcc tgttcagccc cgaccagctg ccgcgcagct tctgcgacac
      241 ctggcaactg acccgcgaac tgggccaggc ggcgctggcc ggcgagaacc tgacggtgga
      301 cagcgcccac ctgccggaaa ccaaccgctt cctcgccgat gtccgctacc tgatcggcgc
      361 catcgccgtg ccgcgcggcg gcgcgctgtt ccgctggaac gagaaggatg gcgccaagga
      421 agacgcgctg cgcgaatgga cgcgccaggg cggccccagc ctggaaccgc tgttcgccgg
      481 ctgcgcctgg cagccgctgc tcgccgacgc ctaccacgcc gcctgccgca acgccgaccg
      541 cggctcgcgc ccgtactcgg tgaaggcgtc tatcgccttc ctgcagacca cgctcggcct
      601 gatgcccgac gccatccgcg ccgtcatcgg cccctgctac gaccggcgca tggaggaata
      661 ccgcgtcggc ctcggcccca aggacggcga cgccgtctac cacggcatcg tctggccgct
      721 gctcggcggc gaggacgaga gtaccgactg cgccggcgag atcgaagcgg cgctgcgcga
      781 agccggggtc aaggacgtgc gcttcctcga ccaccacttc ccgatggagt tctgcgacga
      841 ctgcggcgcg ccaatgttcc ccaacatcga cggcgaactg gtgcacgccg aaatgccgga
      901 acagccgtcg agcagctcgc agcacctgca ctgatgagcg gcaacgccga ctggctggcg
      961 cgcagccagg ctgccgtctg gcacccatgc acgcagatgc gccaccacgc cgaccaggcg
     1021 tcgccggcgc acctgccgct ggttcccatc gcccgcggcg cggggccgtg gttgtacgac
     1081 ttcgacggca agcgctacct cgacgggatc agctcgtggt ggaccaacct gttcggccac
     1141 gccgagccgc gcatcaacgc ggcgctgcgc gagcaactgg aaaccctgga gcacgtcatg
     1201 ctggccggct tcacgcaccg gccggtggtc gaactgtccg aacgcttgag tgatctgacc
     1261 ggcggcgcgc tcggccacgc cttctacgcg tccgacggcg cctcggcaac cgagatcgcg
     1321 ctgaagatga gcttccacta ctggcgcaat gtcggccgcc cggaaaaggc ggagttcctc
     1381 tgcctcgccg gcagctacca cggcgagacg gtgggcgcgc tggcggtcac cgacgtcgcc
     1441 atcttcaagg acgcctacgc gccgctggtg cgccacgcgg cgcatgtgct gccgtcgccc
     1501 gacttccgcc aggcggaagc cggcgaaacc ccggccgacg tcgcccgccg cgccgccgcc
     1561 ggccttgccg actggctgga gaaaaacggt gcgcgcaccg ccgcgctgat cgtcgagccg
     1621 ctggtccagg gcgccgccgg catggcgatg tccgacccgg aatacctgcg cctcgcccgc
     1681 cagttgtgcg accagcacga agtgcacctg atctgcgacg agatcgccgt cggctgcggc
     1741 cgtaccggca ccttcttcgc gcatgaacaa gcggagattc gccccgacct gatctgcctc
     1801 tccaagggca tttccggcgg ctacctgccg atgtcggtgg tgctgtgcag cgacgccatc
     1861 tacgccgcct tcctcgacga ttcggtggcg cgcgccttcc tgcactcgca ttcctacacc
     1921 ggcaacccgc tcgcctgccg ggcagcgctg gcgaccctgg atattttcga aagcgacgag
     1981 gtgctggcca ccaaccgcgt gaaagcgcag aaaatcggcg ccgccctggc gccgctcgcc
     2041 gcccacccgc aggtcatcca cctgcgccag cgcggcatga tcgccgcctt cgacgtagtc
     2101 accgacgacc cgcatttctc gcgccgtttc taccgcgccg cgctcgacgc cggcgcgctg
     2161 ctgcggccga tcggcaacac cgtctacctg atgccgccct acgtcgtcag cgacgacgaa
     2221 atcgcgctgc tcggccgggt cgccaggcaa gcgctggaga ttgcgctgcg atgaccggac
     2281 tgttcctgaa atccctgctc ggtgcggcgg cggtgctgat catcgcgctg ctctcgcgct
     2341 cgaagagctt ctacatcgcc ggcctggtgc cgctgtttcc gaccttcgcg ctgatcgccc
     2401 acgccatcgt cggcgtcgaa cgcggcatga ccgacctgcg cacgacggcg ctgttcggcc
     2461 tctggtcgct gatcccctac gccgtctacc tgctcgctgt gtactggctg agcgcgcgcc
     2521 tggcgctggc gccgacgctg atcagcgcca ccctggcctg ggtcgtcgcg gcggcgctgc
     2581 tgctcttcgt ctggatgcgc gtgtacggca gcgcctaggg cgtgttcaca gtaaccagcg
     2641 ggacggcgtt tgccgtggtg ggaagggcgc caaggcgcga ggcgctggga atggttgttc
     2701 cattcccgag ccgagtaacg ccggcgaccg cccgccacgg caaacccgaa gggcaagggg
     2761 ttggcggggc gcattgccgc gttgccgctc gcttgtgcag atcactgcac ggcgcgaccg
     2821 gcgccttgca ctgcctcccc gccaacccct tgcgccgccc gctgcttact gtgaacacgc
     2881 cctaagcccc gccctcggcg acctggacca agagcagcag gagttcgcgc cgcttttcgt
     2941 cgcgttgatc gcccgccggc agcgactcga cctcggcacg caaagtggcg atttcgcggc
     3001 gcagcagcac ctcgcgcggg gtgaaaccgg cgttcttcag aatcctgttg accatgcgct
     3061 gctcgggcga gagcaagggc tcgtcgtcaa agaccagcgg cttgccggcc cccggcaggt
     3121 ggtcgagatc gccgcgcgcc agcgcctcgc tgatgcgccg ttcggcaagc tgttcgaaaa
     3181 ttccggccat gcgcgctagc cagggggtca ggc
//
LOCUS       DAOSDX010000261         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10620_length_3208_cov_3.656200, whole genome shotgun sequence.
ACCESSION   DAOSDX010000261 DAOSDX010000000
VERSION     DAOSDX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10620_length_3208_cov_3.656200"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1709)
                     /locus_tag="PLN02_06785"
     CDS             complement(18..1709)
                     /locus_tag="PLN02_06785"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOI52068.1"
                     /translation="MTTTSASPESLIDFAAFNLGEDELRSLVSLAPLWKSSVTAECAR
                     SEPDVALQHFIELTVALPGGIADPDWQQRWIRAWHELLGRGYGQVEILRWFFNIVDAC
                     EKILLGGRDSVGRVLLSLLGTLRRSVIAAITCVLEVDELAQQEAGGLPGELAALRFLR
                     ELLQGGRVVGLLSVSLANRDSFTYLDANDLQRLPATLARRMRRHLREEDRVFVGREGE
                     WLVVLPDIKVMSQPTLAAAHLERMFGEPVPLFSGRKVMVRSTIGAALLPQHGASAETA
                     LHAARLARWGAQGRRQSFAWFDAGFAESWQQRHELAEELREALFQETLELHIQPQNDL
                     ADGHCIGGELLLRWRHERTGWVPPPLIMEMIEENGWRLMFNDWLLRAAMRAAAELEKA
                     GIGIGLSFNLTAADMLDDDLPEFLEQNLLTWRIPGERFTLELTESALMFDRAKGSSIM
                     HRLRKLGCRIALDDFGTGYSSLSYLVSLPLNELKVDRSFVVAMLDSADSLRVVSTIVD
                     LARDLGMMPLAEGIEDEQQRVQLLALGCVAGQGYLYAQPMTIEQFIVWYHSERQH"
     gene            complement(1690..2994)
                     /locus_tag="PLN02_06790"
     CDS             complement(1690..2994)
                     /locus_tag="PLN02_06790"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOI52069.1"
                     /translation="MEIIPVAELKIGMFITEPDCPWTEFSFALQGFIISKPEQVEIFR
                     KKCRFVQIDRSRSLNEHFVAPKERVDRQLQAAPLSGAGSQEEDVYGEVQPLSAEQVVL
                     RQRRRRFLELLHGQEGDEHVRSLTRELDYIEPRYDDLLEALKQTFRNVTAEMQFDFAN
                     VREGVRDIAGSLKRNPDAVMWLLRLKSLDQYSFDHAMDVAVHALLLAGHIGWRGQQLL
                     ELGIAGLLQDVGKTQVPSEVLAKKEALTEAERQLVRSHVASSLEILTTQGKLPGEVLQ
                     IVSRHHERWDGSGYPRGLRFERIGLAAEIAGLVDAFCAMLRNKPYRNAIGHQEALEEL
                     FQQRGKQFNPVLMEQFVQCVGLYPIGVLVEFESGEVGVVIQQNRVQRSRPRVLLMLDH
                     DKQPVRDFRLVDLRQPELAALRIARALPNDAYGLSSHDYYLG"
     gene            complement(2998..>3208)
                     /locus_tag="PLN02_06795"
     CDS             complement(2998..>3208)
                     /locus_tag="PLN02_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOI52070.1"
                     /translation="DAIVSGAIEAQVGAIERAWSRLPGNERHCLLSGGNAGLLQPFLA
                     MSMVRMDNLPMYGLKELARETSRET"
BASE COUNT          590 a         1051 c         1021 g          546 t
ORIGIN      
        1 atctgccgct cccttgttca gtgctgccgc tcgctgtgat accagacgat gaactgttcg
       61 atcgtcatcg gctgcgcgta aagataaccc tgccccgcca cacaaccaag cgccagcaac
      121 tggacgcgct gctgctcgtc ctcgatgccc tcggcgagcg gcatcatgcc gagatcgcgg
      181 gcaaggtcga cgatggtgct gacgacgcgc aggctgtccg ccgaatcgag catggcgacg
      241 acgaaggaac gatcgacctt gagttcattg agcggcaggc tgaccagata actgagcgag
      301 gaataaccgg tgccgaaatc gtcgagggcg atccggcagc ccagcttgcg cagccggtgc
      361 atgatcgacg agcccttggc gcggtcgaac atcagcgccg actcggtcag ttcgagggtg
      421 aagcgctcgc cgggaatgcg ccaggtcagc aggttctgct cgaggaactc cggcaggtcg
      481 tcatcgagca tgtcggcagc cgtgaggttg aacgagaggc cgatgccgat gccggccttt
      541 tccagttcgg cggccgcccg catcgcggca cgcaacaacc agtcgttgaa catcaggcgc
      601 cagccatttt cctcgatcat ttccatgatc agcggcggcg gcacccagcc ggttcgttcg
      661 tgccgccagc gcaacagcag ctcgccaccg atacagtggc cgtcggccaa gtcgttctgc
      721 ggttgaatgt gcagttccag ggtttcctgg aacagggcct cgcgcaactc ctcggccagc
      781 tcgtgacgtt gctgccagct ttcggcaaaa ccggcatcga accaggcaaa ggactggcgc
      841 cgcccctgcg cgccccagcg cgccaggcgg gcggcgtgca gggccgtctc ggcgctcgct
      901 ccgtgctggg gcaggagcgc ggcaccgatg gtcgaccgca ccatcacctt gcgaccactg
      961 aacagcggca ccggctcacc gaacatccgt tcgagatggg cggcggccag ggtcggctgc
     1021 gacatcacct tgatgtcggg cagaacgacc agccactcgc cttcgcgacc gacaaagacc
     1081 cggtcctcct cgcgcagatg gcgcctcatc cgccgcgcca gcgtcgccgg caggcgctgc
     1141 aggtcgttgg cgtcgaggta ggtgaaggag tcgcggttgg ccagcgagac cgagagcagg
     1201 ccaaccaccc ggccgccctg caggagctcg cgcaaaaaac gcagggcggc cagttcgccg
     1261 ggcaggccgc ccgcttcctg ctgcgccagt tcatccactt ccagcacgca ggtgatggcg
     1321 gcgatgacgc tgcggcgcag ggtgccgagc agggaaagca gcacccggcc aacactgtcg
     1381 cgcccgccga gcaggatctt ttcacaggca tcgacgatat tgaagaacca gcgcaggatt
     1441 tcgacctgac cgtaaccccg gcccagcagt tcgtgccagg cacggatcca gcgctgctgc
     1501 cagtcgggat cggcgattcc gccgggcagc gcgacggtca gttcgatgaa atgttgtaac
     1561 gcgacgtccg gctcgctacg cgcgcattcc gccgtcaccg acgacttcca cagcggcgcc
     1621 aggctgacca gggaacgcag ttcgtcctcg cccagattga aggcggcaaa atcgatcagc
     1681 gattccgggc tagccgaggt agtagtcatg cgacgacaga ccataggcat cgttcggcag
     1741 cgcccgggcg atacgcaagg cggccagctc cggctggcgc aggtcgacca gacggaaatc
     1801 acggaccggc tgcttgtcgt ggtcgagcat caacagcacg cgcggccgcg agcgctggac
     1861 gcggttctgc tggatcacca cgccaacctc gcccgattcg aactcgacca gcacgccgat
     1921 cgggtagagc ccgacgcatt gcacgaactg ctccatgagc accgggttga actgcttgcc
     1981 gcgctgctgg aacagttcct cgagcgcttc ctggtggccg attgcgttgc ggtacggctt
     2041 gttgcgcagc atcgcgcaga aggcatcgac caggccggcg atttccgcgg ccaggccgat
     2101 gcgctcgaag cgcaggccgc gcggatagcc gctgccgtcc cagcgctcgt ggtggcggga
     2161 gacgatctgc agcacttcgc ccggcagctt gccctgagtg gtcaggattt ccagcgaact
     2221 ggcgacgtgc gagcgcacca gttgccgctc cgcctcggtc agggcttcct tcttggccag
     2281 tacttcggac ggcacctggg tcttgccaac atcctgcagc agcccggcga tccccagttc
     2341 gagcagttgc tggccgcgcc agccgatatg tccggcgagc agcaaggcat gcaccgcgac
     2401 gtccatggca tggtcgaagc tgtactgatc cagactcttc agccgcagca gccacatcac
     2461 ggcgtcggga ttgcgcttca ggctgccggc gatgtcgcgc acgccttcgc ggacgttggc
     2521 gaaatcgaac tgcatttccg cggtgacgtt gcggaaggtc tgcttcaagg cttcgagcaa
     2581 atcgtcgtag cgcggttcga tgtagtcgag ttcgcgggtc agcgaacgga catgttcgtc
     2641 gccttcctga ccatgcagga gttcgaggaa gcgacggcgg cgctggcgca gcacgacctg
     2701 ttccgcgctc agcggctgga cctcgccata aacgtcttct tcctggctgc cggcgccact
     2761 caagggcgcc gcctgcaact ggcgatcgac gcgctccttg ggcgccacga agtgctcgtt
     2821 cagcgagcgc gagcggtcga tttgcacgaa acggcacttc ttgcggaaga tctcgacctg
     2881 ctcgggcttg ctgatgatga aaccctgcaa ggcgaacgaa aactcggtcc acggacagtc
     2941 cggttcggta atgaacatgc cgatcttcag ttcggcaacc ggaatgattt ccatggatca
     3001 ggtctctctg gacgtttcgc gggccagttc cttgagcccg tacatgggta gattgtccat
     3061 ccgcaccatg ctcatggcaa ggaaaggctg caacaatccg gcgttaccac cggacagcag
     3121 gcaatgccgt tcgttccccg gcagtcgcga ccaggcccgt tcgatggcgc cgacctgcgc
     3181 ttcgatggca ccgctgacga tggcgtcg
//
LOCUS       DAOSDX010000262         3203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10652_length_3203_cov_2.965693, whole genome shotgun sequence.
ACCESSION   DAOSDX010000262 DAOSDX010000000
VERSION     DAOSDX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10652_length_3203_cov_2.965693"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLN02_06800"
     CDS             complement(<1..765)
                     /locus_tag="PLN02_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52071.1"
                     /translation="MRQDSASPLPAVHARPIDLWPIGVSGLLGAVAMAPASLAEAAIA
                     GGFLLACLGLGWFALSRQARAHAARYEAASAACRDETARLAQQGLSGLDRLCLDLLPI
                     WSGQIETARSHTEDAAVSLSTRFADISQRLQQATAGSEAAAGDGNLLQLLNSAQDELH
                     AIIASLRTALGTKESLLAEVGALASHTETLSRMAREVAEIAKQTNLLALNAAIEAARA
                     GDVGRGFAVVADEVRKLSTLSGDTGMKISRTVDTVNH"
     gene            complement(1335..2171)
                     /locus_tag="PLN02_06805"
     CDS             complement(1335..2171)
                     /locus_tag="PLN02_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOI52072.1"
                     /translation="MDVERALQIIVADAERGNMVFPTHAELALSVKRMLDDPECAVEP
                     LTRLIAAEPLLASRVVAMANSVVYNRSGRRSADLRGAVSRLGFNTLRTLTMAIVIGQM
                     ANMVRDPTHRALAARLWEHSVHVAALARVIASRVTRQDPETAFFAGIVHEIGGFYLIA
                     RAAAFPGLLESDLAAWVGEREAQVGRFVLHALDIPENVMNALEVLWSGYLAMPAASLG
                     DTLLLADYLAPVESPLAELTGMGRRGMAAEIEQQVDGAELSAILADSADEVAALIASL
                     KG"
     gene            complement(2176..2715)
                     /locus_tag="PLN02_06810"
     CDS             complement(2176..2715)
                     /locus_tag="PLN02_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOI52073.1"
                     /translation="MKDLPEPLREVVLNPGEFHFGGGRTRISTLLGSCVSITLWHPRQ
                     RIGGMCHFMLSGRQRPAGAPLDGRYADEAFALFLERVAAAATQATAYQAKLFGGANML
                     RDLAGENIDIGRRNVARARELLAAHNIVLLSEHVGGSGRRKLHFDLWSGDVWLAFPDG
                     SDASIRKAGRPDLTSLQEK"
     gene            complement(2712..>3203)
                     /locus_tag="PLN02_06815"
     CDS             complement(2712..>3203)
                     /locus_tag="PLN02_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOI52074.1"
                     /translation="WSAACSSGEEVYTLAMVLAEALGDKPWEIIGSDISTQVLAKAAS
                     GHYSLARTEGIPPGLMKKHCLRGVRSQAGSFMIVPELRQRTRFYQLNLMHPVDTGIGD
                     FEVIFLRNVMIYFDPPTKTRVVHNLLPRLKSGGHLVIGHSETLNGITDRVQAVVPTIY
                     RKP"
BASE COUNT          586 a         1008 c         1075 g          534 t
ORIGIN      
        1 gtggttgacc gtatcgacgg tgcggctgat cttcatcccg gtgtcgccgg agagcgtgga
       61 cagcttgcgc acctcgtcgg cgaccaccgc gaaaccgcgc ccgacgtcgc cagcgcgggc
      121 cgcctcgatg gcggcgttga gcgccagcag gttggtctgc ttggcgattt cggcaacttc
      181 cctggccatc cggctcaacg tctcggtatg actggccagc gcgccaactt cagcgagcag
      241 gctttccttg gtgccgagcg cggtgcgcag cgaagcgatg atggcgtgca actcgtcctg
      301 cgccgaattg agcagttgca gcaggttgcc gtcgccggcc gcggcctcgc tgcccgcggt
      361 tgcctgctgc aggcgttgcg agatgtcggc gaagcgggtg ctcagcgaaa ccgcggcgtc
      421 ctcggtatgc gagcgggcgg tttcgatctg cccggaccag atcggcagca ggtcgagaca
      481 gagccggtcg agaccgctca agccctgttg cgccaggcga gccgtctcgt cacggcaagc
      541 agcgctagct gcctcgtagc gagcggcatg cgcccgcgcc tggcgggaca gcgcaaacca
      601 gccgagcccc agacaggcga gcaggaagcc gccggcaatc gccgcctcgg ccagcgaagc
      661 tggcgccatg gcgacagcgc cgagcagacc actgacgccg atcggccaga ggtcgatggg
      721 gcgggcgtga actgccggaa ggggcgatgc tgagtcctgc ctcatggtcc aattcctcgg
      781 ttgcacaaaa gtaaaaacat ctctgattag gtaacgcaaa acaacagcgc cataatacta
      841 tttttaataa tagtgtcaaa tatattattc gacgccatga attttttgcg cgcgccggta
      901 cgctcgccag acaagacggg gacgaagaag agacacgaaa aagccttgga cagcgtcgaa
      961 aaaaggcgtg agattccgcc aggcgtggcg cgacgacagc cttccggggc accgcagggc
     1021 acaccgggga aactcaggat ctgtcgaggc gggcagcgcg gaatcgagac ggcttcggga
     1081 acgctgcgct tgccgcgccg ctgcgctatg tgtcgctgat gaagttgcct tgaaccattg
     1141 tcttaccccg catcacgatt tgtcttgttc tgattggcag cgccaccgcc ttgcccgtca
     1201 gccttcgagg gcggactggg tgcgaaccac tataacgaag gtaataaacc ggtgcaatgg
     1261 ttttccctag gggttttcat ccgggtgtac cccttttggc aaaacgcgcc ctcgccatcg
     1321 cgcgaaggcg gcgctcagcc cttgagcgaa gcgatcaggg cggcgacttc gtccgccgaa
     1381 tcggcgagga tggcactgag ttcggcgccg tccacctgct gctcgatctc cgccgccatc
     1441 ccccgccgcc ccatgccggt gagttcggcc aacggggatt caactggcgc cagatagtcg
     1501 gcgagcagca gggtgtcgcc cagcgacgcc gccggcatcg ccaggtaacc gctccacagg
     1561 acttccagcg cattcatgac gttttccgga atatcgaggg catgtagcac gaaacggccg
     1621 acctgcgcct cgcgttcgcc gacccaggcc gccagatcgc tttccagcaa gccgggaaaa
     1681 gcagcagcgc gggcgatcag atagaaaccg ccgatttcat ggacgatacc ggcgaaaaag
     1741 gccgtttccg ggtcctggcg ggtgacgcgg ctggcgatca cccgcgccag cgcggcgaca
     1801 tgcaccgagt gctcccagag ccgggcggcg agcgcccggt gcgtcggatc gcgaaccatg
     1861 ttcgccatct ggccgataac gatcgccatc gtcagcgtgc gcagcgtatt gaaaccgagg
     1921 cgcgagacgg cgccgcgcag gtcggcgcta cgccgccccg aacggttgta gaccaccgaa
     1981 ttggccatcg ccacgacgcg gctggcgagc agcggctcgg cggcgatcag ccgggtcagt
     2041 ggctcgaccg cgcactcggg atcgtcaagc atgcgtttga cactgagcgc cagttcggca
     2101 tgggtgggga acaccatgtt gccgcgctcg gcgtcggcga cgatgatttg cagggcgcgt
     2161 tcgacgtcca tggcgtcatt tctcctggag agaggtcagg tcgggccggc cggccttccg
     2221 gatgctggca tcgctgccat cgggaaaggc cagccagaca tcgccgctcc acaagtcaaa
     2281 gtgcagcttg cggcggccgc tgccgccgac gtgctcggac agcaatacga tattgtgcgc
     2341 cgccagcaac tccctggcac gggcaacgtt acggcggccg atgtcgatgt tctcgccggc
     2401 caggtcgcgc agcatgttgg cgccaccgaa cagcttggcc tggtaggctg tggcctgggt
     2461 agcggcggcg gcaacgcgtt cgaggaagag ggcgaaggcc tcgtcggcgt agcggccgtc
     2521 gagcggcgcg ccggccggcc gctggcgacc gctgagcatg aagtggcaca tgccgccgat
     2581 gcgctggcgc ggatgccaga gggtgatcga gacgcatgag ccgagcagcg tcgagatgcg
     2641 cgtccggccg ccgccgaaat ggaattcgcc ggggttgagg acgacttcgc gcagcggttc
     2701 gggcaggtcc ttcatggctt gcggtagatg gtcggcacca cggcctggac acggtcggtg
     2761 atgccgttga gcgtttcgga atggccgatc accaggtgac cgccggactt caaccggggc
     2821 aacaggttat gcacgacccg ggtcttggtc ggcgggtcga aatagatcat cacgttgcgc
     2881 aggaagatca cctcgaaatc gccgatcccg gtatcgaccg ggtgcatcag gttgagctgg
     2941 tagaagcgcg tgcgctggcg caactcgggc acgatcatga agctgccggc ctgcgagcgg
     3001 acgccacgca ggcagtgttt cttcatcaag ccaggcggaa taccctcggt cctcgccagc
     3061 gagtagtggc cgctggccgc cttggccagg acctgggtgc tgatgtcgga accgatgatt
     3121 tcccagggtt tgtcgcccag cgcctcggcc agcaccatgg ccagcgtgta gacctcctcg
     3181 ccgctcgaac aggccgcgct cca
//
LOCUS       DAOSDX010000263         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10694_length_3195_cov_3.851274, whole genome shotgun sequence.
ACCESSION   DAOSDX010000263 DAOSDX010000000
VERSION     DAOSDX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10694_length_3195_cov_3.851274"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="bioD"
                     /locus_tag="PLN02_06820"
     CDS             <1..571
                     /gene="bioD"
                     /locus_tag="PLN02_06820"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOI52075.1"
                     /translation="LWAVGLKPVAAGTDAAGHNDDVQRLRAASSLQLPDEVVNPYCFA
                     PAIAPHIAAAEAGVDIDFTRIASTVASARQAADFVVVEGAGGFCVPFGLDRNGADLAA
                     QLRLPVILVVGMRLGCLNHALLTAEAVAARGLVIAGWVANCLAPPMPYLEENLATLSE
                     LLLAPRLGVVSWDETGKVENAASQLELPF"
     gene            complement(560..910)
                     /locus_tag="PLN02_06825"
     CDS             complement(560..910)
                     /locus_tag="PLN02_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOI52076.1"
                     /translation="MTTTPPFAIDKPAIMERLGGDREIYAMMIDIFLQDVEHNCSNLL
                     AASLSGSAELLVREAHTVKGLLATMSDDHGAELALALEMQARQGVMDGAEAKAQGIVA
                     RINEVAAVLRVSER"
     gene            complement(934..1986)
                     /locus_tag="PLN02_06830"
     CDS             complement(934..1986)
                     /locus_tag="PLN02_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52077.1"
                     /translation="MHKVLIIDDSDINLTLIKALVLKLGDCEPILFERPLEALEWCRD
                     NVPDLVIVDYMMPDMDGLRFIAAFRALHGRTEIPVLMITANDQKDIRYEALLGGANDF
                     LTKPIDRIEFSARSRNMLALRTGQKFLADRAAYLAEKVEERTREIRERERELIFRISR
                     AAEFRDPETGAHIQRMAHYSEIIARGLDLDDASCRLLLKAAPMHDVGKIGIPDYILLK
                     PGRLTPQEFEVMKRHAELGYELLKDSGSEILQAGAEIALSHHEKYDGSGYPKGLKGHA
                     IPLFGRIVAVADVFDALTSERPYKKAWSLDDALRFLENGRGAHFDPLCVEALLAGWEE
                     VLEVRQRFHDEEMPLI"
     gene            2036..2644
                     /locus_tag="PLN02_06835"
     CDS             2036..2644
                     /locus_tag="PLN02_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HOI52078.1"
                     /translation="MKLLFDLFPVILFFAAFKYAGGHPETAADWVGSLLGGAVIAPDK
                     APILLATVVVIVATVAQIAWVHFRHGKVDKMLWLSLGLVVVFGGMTLIFRNEAFIKWK
                     PTLLYWAFAGSMLFAALIMKKNAIQAMLGEQMKLPDAVWRRLNFAWIGFFLVMGVLNL
                     IIAFNFPTDIWVDFKLFGGMGLMLVFIVAQGALLSKYIEEEK"
     gene            2644..2946
                     /locus_tag="PLN02_06840"
     CDS             2644..2946
                     /locus_tag="PLN02_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOI52079.1"
                     /translation="MLYMIVGEDRPGSLEQRLAARPEHLARLQALQADGRLLLAGPCP
                     AIDAPDPGAAGFSGSLIVAEFASLDAARAWADADPYIAAGVYEKVTVKPFKKALPA"
     gene            2943..>3195
                     /locus_tag="PLN02_06845"
     CDS             2943..>3195
                     /locus_tag="PLN02_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOI52080.1"
                     /translation="MSHEATVALLRERLSALAPQSLAIADESHRHAGHASAGGGAHFR
                     VDIVAAAFSGKKTLVRHRLVYDAAGDLMHHRIHALAIRAL"
BASE COUNT          526 a         1073 c          992 g          604 t
ORIGIN      
        1 gctctgggcg gtcggcctca agccggtcgc cgccggcacc gacgccgccg gccacaacga
       61 cgacgtgcag cgcctgcgcg ccgcaagcag cctccagttg ccggacgagg tggtcaatcc
      121 ctattgtttc gcgcccgcca tcgcgcccca catcgccgcc gccgaagccg gcgtcgatat
      181 cgatttcacg cgcatcgcca gcaccgtggc gagcgcccgc caggcggcgg atttcgtcgt
      241 cgtcgaaggc gccggcggct tctgcgtacc gttcgggctc gaccgcaacg gcgccgatct
      301 cgccgcgcaa ctgcggctgc cggtgatcct ggtcgtcggc atgcggctcg gctgcctcaa
      361 ccacgcgctg ctcaccgccg aagcggttgc cgcgcgcggc ctggtcatcg ccggctgggt
      421 ggcgaactgc ctggcgccgc cgatgcctta cctggaagaa aatctggcga cgctgagcga
      481 actgctgctg gcgccaaggc tgggggtcgt ctcctgggat gagacgggca aggtcgagaa
      541 cgctgccagc cagctcgaac taccgttctg agacgcgcag caccgcggcg acttcgttga
      601 tccgcgcgac gatgccctgc gccttggcct cggcaccgtc catcacaccc tggcgggcct
      661 gcatttccag cgccaacgcc agttcggcgc catggtcgtc ggacatcgtc gccagcaagc
      721 ccttgaccgt atgcgcctcg cgcaccagta attcggcgct gccggagaga ctggccgcca
      781 acaggttgct gcaattgtgc tcgacgtcct gcaggaagat gtcgatcatc atcgcgtaga
      841 tctcccgatc accgcccaga cgctccatga ttgccggctt gtcgatggcg aagggtgggg
      901 tggtggtcat tgcagtctcc attcgacgct gtatcagatc agcggcattt cctcgtcatg
      961 gaagcgttgc cgcacttcga gcacttcctc ccaaccggcc agcaaggctt cgacgcagag
     1021 gggatcgaag tgcgcgccac gcccgttttc gaggaaacgc aacgcatcgt ccagcgacca
     1081 ggctttcttg tatgggcgtt ccgaggtcag cgcatcgaag acgtcggcga cggcgacgat
     1141 acgaccgaac aacgggatgg cgtgcccctt gagtccttta ggatagccgc tcccgtcata
     1201 tttttcatgg tgggaaaggg cgatttcggc gcccgcctgc aggatttccg atccgctgtc
     1261 cttgagcaac tcatagccca gttcggcgtg ccgtttcatg acttcgaact cctgcggcgt
     1321 caaacggccg ggtttgagca ggatgtagtc gggaatgcca atcttgccga catcgtgcat
     1381 cggcgccgcc ttcagcagca gccggcagct ggcatcgtca aggtcgagcc cgcgggcgat
     1441 gatttccgag tagtgcgcca tgcgctggat atgggcaccg gtttcgggat cgcggaattc
     1501 ggcggcgcgc gaaatccgga agatcagctc gcgttcgcgc tcgcggattt cccgggtacg
     1561 ttcctcgacc ttttcggcca gataggcggc gcgatcggcg aggaatttct ggccagtgcg
     1621 caaggccagc atgttgcgcg aacgggccga gaattcgatg cggtcgatcg gcttggtcag
     1681 gaaatcgttg gcgccgccga gcagcgcttc gtaacggatg tccttctggt cattggcggt
     1741 gatcatcaac accgggatct cggtccgccc atgcagcgcg cggaaggcgg cgatgaaacg
     1801 caggccgtcc atgtccggca tcatgtaatc gacgatgacc aggtcgggca cgttgtcgcg
     1861 acaccattcg agcgcttcca gcgggcgctc gaaaaggatc ggttcgcaat ccccgagctt
     1921 gaggaccagc gccttgatca gcgtcaggtt gatgtcgctg tcgtcgatga tcagtacctt
     1981 gtgcattcgt ctgcccgagc gttgcgtttc tcggcgcagt atataatgcg ccgccatgaa
     2041 actcctcttc gatctcttcc cggtcatcct ctttttcgcc gccttcaaat acgctggcgg
     2101 ccatccggaa accgccgccg actgggtcgg cagcctgctc ggcggcgccg tcatcgcccc
     2161 cgacaaggcg ccgatcctgc tcgccaccgt cgtcgtcatc gtcgccaccg ttgcccagat
     2221 cgcctgggtg catttccgcc acggcaaggt ggacaagatg ctctggctca gcctcggcct
     2281 ggtcgtcgtc ttcggcggca tgacgctgat cttccgcaac gaggccttca tcaagtggaa
     2341 gccgacgctg ctctactggg ccttcgccgg cagcatgctg ttcgccgcgc tgatcatgaa
     2401 gaagaacgcg atccaggcca tgctcggcga gcagatgaag ttgccggacg ccgtctggcg
     2461 gcggctcaac ttcgcgtgga tcggcttctt cctggtcatg ggcgtgctca acctgatcat
     2521 cgccttcaat ttcccgaccg acatctgggt cgatttcaaa ctcttcggcg gcatgggctt
     2581 gatgctcgtc ttcatcgtcg cgcagggcgc gctgctgtcg aagtacatcg aggaagaaaa
     2641 atgatgctgt acatgatcgt cggcgaagac cgccccggca gcctggagca acgcctcgcc
     2701 gcccgccccg agcacctcgc ccgcctgcag gcgctgcagg ccgacggccg cctgctgctg
     2761 gccggcccct gcccggcgat cgacgcgccc gaccccggcg ccgccggttt cagcggcagc
     2821 ctgatcgtcg ccgaattcgc ctcgctcgac gccgcccgcg cctgggcaga tgctgatccc
     2881 tacatcgccg ctggcgtcta cgagaaggtc accgtcaagc cgttcaagaa ggcgctgccg
     2941 gcatgagcca cgaggccacc gtcgccctgc tgcgcgaacg cctgagcgcg ctggcgccgc
     3001 aatcgctcgc catcgccgac gaatcgcatc gccacgccgg ccatgccagt gccggcggcg
     3061 gcgcgcattt ccgcgtcgac atcgtggcag cggctttttc cggcaaaaag acactcgttc
     3121 gtcatcggct ggtgtacgat gccgcaggtg acttgatgca ccaccggatc cacgcgctgg
     3181 ccatacgcgc actgg
//
LOCUS       DAOSDX010000264         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10747_length_3182_cov_3.050208, whole genome shotgun sequence.
ACCESSION   DAOSDX010000264 DAOSDX010000000
VERSION     DAOSDX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10747_length_3182_cov_3.050208"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLN02_06850"
     CDS             <1..399
                     /locus_tag="PLN02_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOI52081.1"
                     /translation="LPLLTALCDAGYDVSLETSGAFDIAPVDPRVSCIMDLKAPDSGE
                     AAKNLWTNLAHLKPRDEIKIVIASREDYEWAREVVHAHGLDARCPVLFSPAQGSVEPA
                     ALAGWILEDGLPVRFQLQLHKLLWGNMQGK"
     gene            415..777
                     /locus_tag="PLN02_06855"
     CDS             415..777
                     /locus_tag="PLN02_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOI52082.1"
                     /translation="MAENRRQFTRIVFSGPARLFLADGEHPVDVLDLSLKGALIRPCA
                     EMYTRIGGQGVLHLRLDGGETDIRMAVTVVHHQGDTYGLACHEIDLDSVTHLRRLVEL
                     NLGDETVLEREIGLLSRA"
     gene            879..2600
                     /locus_tag="PLN02_06860"
     CDS             879..2600
                     /locus_tag="PLN02_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI52083.1"
                     /translation="MSNFEFPEASADNSVAPESQHAILNQIGAAVMLTDPAERILWIN
                     EAFSRLTGYQPEEIIGRTPKILASGRQSPIFYSHMWQALDSAGHWEGEIWNRRKNGEI
                     YPEWLIIRRSDDANGETTGYVATFSDISKLKHAESEVQHLAFYDPLTALPNRRLLRDR
                     LAQACAAGKRSSGHGALLIIDIDNFKILNDTLGHDIGDHLLIEIACRLRQSIRQCDTA
                     ARQGGDEFAVMLEDLDPHPQSAAVSAEAIAEKIREALSHPVALTEGHEHYCTASIGVT
                     LFRGGEKSVDTLLKQADIALYKAKESGRNATRFFDQAMQTTLDHRAGIEAGLRHALAR
                     EEFVLYAQPQVNAEGTLIGAELLLRWQPPGQALVLPNDFIPLAEESGLIVPIGLRVLH
                     SACATLHRWAADPRRAGLALAVNVSARQFRQPDFVEQVASALSYFSIAPQRLKLELTE
                     SLLLDKVDEVVAKMRALHALGVRFSLDDFGTGYASLAYLKRFPFEQLKIDRSFIRDIA
                     SDPDDAAIVRAIVAMGDALRLDVVAEGVENDAQLAYLTEHGCRAFQGYLFGRPVPLVE
                     FETRLES"
     gene            complement(2601..>3182)
                     /locus_tag="PLN02_06865"
     CDS             complement(2601..>3182)
                     /locus_tag="PLN02_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI52084.1"
                     /translation="TTTLRALCGLQPVRAGSIRFAGERIDGWRPDRIGRAGIALVPEG
                     RQCFPNLTVDEHLLAFQANRRGVAGPWTPARVYELFPRLAERRKNLGNQLSGGEQQML
                     AIGRALVTNPRLLVLDEATEGLAPLIRDEIWACLDRLRGAGQSVLVVDKYVEKLIALA
                     DRHTIVERGRVVWAGSSAELDADHAIWHRYLGL"
BASE COUNT          584 a         1099 c          984 g          515 t
ORIGIN      
        1 ctgccgctgc tgacggcgct gtgcgacgcc ggctacgacg tctcgctgga aacctccggc
       61 gccttcgaca tcgccccggt cgacccgcgg gtttcctgca tcatggacct caaggccccc
      121 gattccggcg aagcggcgaa gaacctgtgg accaacctgg cgcaccttaa gccgcgcgac
      181 gagatcaaga tcgtcatcgc ctcgcgcgag gactacgaat gggcgcgcga ggtcgtccac
      241 gcgcacggcc tggacgcgcg ctgcccggtc ctcttttcgc cggcgcaagg cagcgtcgag
      301 ccggcggcac tggccggctg gatcctcgaa gacggcctgc cggtgcgctt tcagttgcag
      361 ttgcacaagc tgctttgggg taacatgcag ggcaaatagc ccccggagac aaacatggcc
      421 gaaaatcgca ggcaattcac ccgcatcgtg ttttccggac cggcccgcct gttcctggct
      481 gacggcgaac atccggtcga cgtcctcgac ctctccctca aaggcgccct gatccgtccc
      541 tgcgccgaga tgtacacccg catcggcggc cagggtgtgc tgcatctgcg cctcgacggc
      601 ggcgaaaccg acatccgcat ggcggtcacc gtcgtccacc accagggcga tacctacggc
      661 ctggcctgcc acgaaatcga cctcgacagc gtcacccacc tgcgccgcct ggtcgaactc
      721 aatcttggcg acgaaaccgt cctggaacgc gaaatcggcc tgctgtcccg ggcttgatga
      781 gaagcgttct tgctggccag acctgactta aggcatactc cgtttatacc taaaaaagat
      841 ggaaattgcc tttagaaccg caggcatgag ggagtgtcat gtcgaatttc gagtttccgg
      901 aggcctccgc tgacaattcg gtcgcgccgg aaagtcagca cgccatcctc aaccagattg
      961 gtgctgccgt catgctcacc gatcccgccg aacgcattct ctggatcaac gaggcgttca
     1021 gccgtctgac cggctaccag ccagaggaaa tcatcggccg cacgccgaag atcctcgcat
     1081 ccggacgcca gtcgccaatc ttctattcgc acatgtggca ggccctcgat agtgccggac
     1141 actgggaagg cgaaatctgg aaccggcgca agaacggcga aatctatccg gaatggctga
     1201 tcatcaggcg cagcgacgac gcgaacggcg aaaccacggg ctacgtggcg actttctccg
     1261 acatcagcaa gctgaaacat gccgaatccg aagtgcagca tctcgccttc tatgatccgc
     1321 tgaccgcgct gcccaaccga cgcctgctgc gtgaccgact cgcccaggcc tgcgcggcag
     1381 gcaagcgcag cagcggtcac ggcgccctgc tgatcatcga catcgacaac ttcaagattc
     1441 tcaacgacac cctcggccac gacatcggcg accacctgct gatcgagatc gcctgtcgcc
     1501 tgcggcaatc gatccggcaa tgcgacaccg cggcgcgcca gggtggcgac gagttcgcgg
     1561 tgatgctcga ggacctcgat ccgcacccgc agtcggcggc ggtcagcgcc gaagcgatcg
     1621 ccgagaaaat ccgcgaggca ttgtcgcatc cggtagcgct gaccgagggg catgagcatt
     1681 actgcaccgc gagcatcggc gtcaccctgt tccgcggcgg cgaaaagtcg gtcgacaccc
     1741 tgctcaagca agctgatatc gccttgtaca aggccaagga aagcggccgc aacgcaaccc
     1801 gcttcttcga ccaggcgatg cagacgacgc tcgaccaccg cgccggcatc gaagccggtc
     1861 tgcgccacgc gctggcgcgc gaggaattcg tcctctatgc ccagccgcag gtcaatgccg
     1921 aaggcaccct gatcggcgcc gaactgctgc tgcgctggca gccaccggga caagcgctgg
     1981 tgctgcccaa cgatttcatt ccgctcgccg aggaaagcgg gctgatcgtg ccgatcggcc
     2041 tccgggtgct gcacagcgcc tgcgccacgc tgcaccgctg ggccgccgat ccacgacgcg
     2101 ccggcctggc gctggcggtc aacgtcagcg cccgacaatt ccgccagccg gatttcgtcg
     2161 agcaggtggc cagcgcactg agctactttt ccattgcgcc gcaacgcctc aagctggaac
     2221 tgacggaaag cctgctgctc gacaaggtcg acgaggtggt ggccaagatg cgggcactgc
     2281 acgcgctcgg cgtccgtttc tcgctcgacg atttcggcac cggctacgcc tcgctcgcct
     2341 acctgaagcg cttccccttc gagcaattga agatcgaccg ttccttcatc cgcgatatcg
     2401 ccagcgaccc ggacgacgca gcgatcgtcc gcgccatcgt cgccatgggc gacgcgctgc
     2461 gcctcgatgt cgtcgccgaa ggggtcgaga acgacgccca gctcgcctac ctgaccgagc
     2521 acggctgccg cgccttccag ggctacctgt tcggccggcc ggtaccgctg gtggaattcg
     2581 agacccgtct cgaaagctga tcagagcccg aggtaacggt gccagatcgc gtgatcggca
     2641 tcgagttcgg ctgaactgcc cgcccagacc acgcgaccgc gttcgacgat ggtgtggcgg
     2701 tcggccaggg cgatcagctt ttccacgtac ttgtcgacca ccagcacgct ctgtcccgcg
     2761 ccgcgcaggc ggtcgaggca ggcccagatc tcgtcgcgga tcagcggcgc caggccctcg
     2821 gtcgcctcgt cgaggacgag cagacgcggg ttggtgacga gcgcccggcc gatcgccagc
     2881 atctgctgtt cgccgccgga cagctggttg ccgaggtttt tgcggcgctc ggcgaggcgc
     2941 gggaagagtt cgtagacgcg ggccggcgtc cacggcccgg caacgccgcg ccggttggcc
     3001 tggaaggcaa gcagatgctc gtcgaccgtc aggttgggaa aacactggcg gccttccggc
     3061 accagcgcga tgccggcacg gccgatgcgg tccgggcgcc agccgtcgat ccgctcgccg
     3121 gcgaagcgga tgctgccggc acgcaccggc tgcaggccgc acagggcgcg cagcgtcgtc
     3181 gt
//
LOCUS       DAOSDX010000265         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10751_length_3181_cov_3.554063, whole genome shotgun sequence.
ACCESSION   DAOSDX010000265 DAOSDX010000000
VERSION     DAOSDX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10751_length_3181_cov_3.554063"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLN02_06870"
     CDS             <1..440
                     /locus_tag="PLN02_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOI52085.1"
                     /translation="ECPAADVVAVDLSPAALAVARNNAGRLGAAVRCVDSDWYAALGN
                     ERFDLIVANPPYIAADDPHLSGDGLPCEPQMALTDGGDGLNCIRVIVAGAAAHLTAGG
                     WLLFEHGYDQGAASRNLLAAAGFQDAFTQPDLAGNDRVSGGHL"
     gene            457..780
                     /gene="grxD"
                     /locus_tag="PLN02_06875"
     CDS             457..780
                     /gene="grxD"
                     /locus_tag="PLN02_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HOI52086.1"
                     /translation="MSDVQQRIHATVTGNPVVLYMKGDARFPQCGFSAAAVQILKACG
                     VNDFVTVNVLADEEIRQGVKDYANWPTIPQLYIKGEFVGGCDIMKEMYAAGELQQMLA
                     DVVSA"
     gene            complement(840..1196)
                     /locus_tag="PLN02_06880"
     CDS             complement(840..1196)
                     /locus_tag="PLN02_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOI52087.1"
                     /translation="MSSKSYRAVTTDLTKALGTLRKELPEATQGFSAMHNGAMKAGAL
                     SELQKELIALAIGIAQHCDGCIGFHVKALIRLGVTREQFLETLAVSIYMGGGPGYMTA
                     AEALRAYEEFTAEKAA"
     gene            complement(1282..2667)
                     /gene="argH"
                     /locus_tag="PLN02_06885"
     CDS             complement(1282..2667)
                     /gene="argH"
                     /locus_tag="PLN02_06885"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOI52088.1"
                     /translation="MTSNANQYTWAGRFSEPVSDLVKRYTASVDFDQRMWRQDIRGSL
                     AHARMLARQGIISAQDLADIERGMAQVAEEIESGQLAWSIDLEDVHLNIEKRLTALVG
                     DAGKRLHTGRSRNDQVATDIRLYLRDAIDDIQVLLKAFRSALIDLAEKEAATPMPGFT
                     HLQVAQPVTFGHHVLAYFEMFGRDAERFADARKRVNRLPLGAAALAGTTYPIDREYVA
                     DQLGFEAVCENSLDAVSDRDFAIEFTAACALLMMHISRLSEELVMWMSPRVGFIQIAD
                     RFCTGSSIMPQKKNPDVPELARGKTGRVYGQLISLLTLMKSQPLAYNKDNQEDKEPLF
                     DAVDTVTDTLRIFADMAGGITVRADNMQAALTQGFATATDLADYLVKKGLPFRDAHEA
                     VGHAVKAAEFKGVDLPGLTLEEFRQFSPLIDDDVYTVLTVEGSLASRKHIGGTAPEQV
                     RAAIARARNRL"
     gene            2729..>3181
                     /locus_tag="PLN02_06890"
     CDS             2729..>3181
                     /locus_tag="PLN02_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOI52089.1"
                     /translation="MTSIRHFPATQPLPDWRNFGVMLRVLLGINLLAAVAALIQSPDL
                     ASWGERFVAMASVVEPLLLIGLAVLSLCRDLLWRLRLRVGQACVLALAGGLAAVLFAY
                     GHSLHLVEAEAVTRGSLLAMTATGMLLLYFELRSRALSPAQAEARLAAL"
BASE COUNT          488 a         1022 c         1090 g          581 t
ORIGIN      
        1 tggagtgtcc ggcggccgac gtcgtcgccg tcgatctctc gccggccgcg ctcgccgttg
       61 cccgcaacaa tgccggccgt ctcggcgccg ccgtgcgctg tgtcgacagc gactggtacg
      121 cggcgctggg caacgagcgt ttcgacctga tcgtcgccaa cccgccctac attgccgccg
      181 acgacccgca cctgagcggc gatggcctgc cctgcgagcc gcagatggcg ctcaccgacg
      241 gcggcgacgg gctgaactgc atccgcgtca tcgttgccgg cgccgcggcg catctgacgg
      301 ccggcggctg gctgctgttc gagcatggtt acgaccaggg cgcggcgagc cggaacttat
      361 tggccgccgc cgggttccaa gacgcattca cgcaacccga cctggccggc aacgaccgtg
      421 tttccggcgg gcacctgtaa tgcaactgga gtccccatgt ccgacgtaca acaacgcatc
      481 cacgcaaccg tcaccggcaa cccggtcgtc ctctacatga agggcgacgc ccgcttcccg
      541 cagtgcggct tttccgctgc cgcggtgcag atcctgaagg cctgcggagt gaacgacttc
      601 gtcacggtca acgtcctggc cgacgaggaa atccgccagg gggtcaagga ctacgccaac
      661 tggccgacca tcccgcagct ctacatcaag ggcgagttcg tcggcggctg cgacatcatg
      721 aaggaaatgt acgcggccgg cgaattgcag cagatgctgg ccgacgtcgt cagcgcctga
      781 gcggcatggc agcatgaata agccccggca attgcccggg gctttttaac gggcgccgtt
      841 caggcggcct tctcggcagt gaattcctcg taggcgcgta acgcctcggc ggccgtcatg
      901 tagcccgggc cgccgcccat gtagatgctc accgccagcg tttccaggaa ctgctcgcgg
      961 gtcacgccga gacggatcag ggccttgacg tggaagccga tgcagccgtc gcaatgctgg
     1021 gcgatgccga tcgccagcgc gatcaattcc ttctgcagtt cgctcagcgc accggccttc
     1081 atcgcgccgt tgtgcatcgc cgagaaaccc tgggtggctt ccggtagttc cttgcgcagg
     1141 gtgccgaggg ccttggtcag gtcggtggtg acggcgcggt aggatttgct gctcatgcgt
     1201 tctcccgata ggttaaatta gagaacgcta attttaaccg gaaagcgcgt cagccacgct
     1261 ttccggtgcc gtgcgccagg ctcagaggcg gttgcgggcg cgggcgatgg cggccctcac
     1321 ctgctccggc gccgtgccgc cgatatgctt gcggctggcc agcgaaccct cgacggtgag
     1381 cacggtatag acatcgtcgt cgatcagcgg cgagaactgg cggaactcct ccagcgtcag
     1441 gccgggcaga tcgacaccct tgaactcggc cgccttcacc gcgtggccga cggcttcgtg
     1501 ggcgtcgcgg aagggcaggc ccttcttgac caggtagtcg gcgaggtcgg tggcggtggc
     1561 gaagccctgg gtcagcgcgg cctgcatgtt gtcggcgcgc accgtgatgc cgccggccat
     1621 gtcggcgaag atgcgcaagg tgtcggtgac cgtgtcgacg gcgtcgaaca gcggctcctt
     1681 gtcttcctgg ttgtccttgt tgtaggcgag cggctgcgac ttcatcaggg tcagcaggct
     1741 gatcagctgg ccgtacacgc ggccggtctt gccgcgcgcc agttccggca cgtccgggtt
     1801 cttcttctgc ggcatgatcg acgagccggt gcagaagcgg tcggcaatct ggatgaagcc
     1861 gacgcgcggg ctcatccaca tcaccagttc ttccgacagg cggctgatgt gcatcatcaa
     1921 gagggcacag gcggcggtga attcgatcgc gaagtcgcgg tccgacacgg cgtccagcga
     1981 gttctcgcag acggcctcga agccgagttg gtcggcgacg tattcgcggt cgatcgggta
     2041 ggtcgtgccg gccagcgcgg cagcgcccag cggcaggcgg ttgacgcgct tcctggcgtc
     2101 ggcgaaacgc tcggcgtcac ggccgaacat ctcgaaatag gccaggacat ggtggccgaa
     2161 ggtcaccggt tgcgccacct gcagatgggt gaagccgggc atcggcgttg ccgcttcctt
     2221 ctcggcgaga tcgatcagcg cgctgcggaa ggccttcagc agcacctgga tgtcgtcgat
     2281 cgcgtcgcgc aggtagaggc ggatgtcggt cgccacctga tcgttgcgcg agcggccggt
     2341 gtgcaggcgc ttgccggcgt cgccgacgag cgcggtgagg cgtttctcga tgttgaggtg
     2401 tacgtcttcc aggtcgatcg accatgccag ctggccggac tcgatttcct cagcgacttg
     2461 cgccatgccg cgctcgatgt cggccagatc ctgggcgctg atgatgcctt gccgggcgag
     2521 catccgggcg tgcgcgagcg agccccggat gtcctggcgc cacatgcgct ggtcgaaatc
     2581 gaccgaggcc gtgtagcgtt tgacgagatc ggagaccggc tcggagaagc ggccggccca
     2641 ggtgtattgg ttggcgttgg aagtcatgac ttgggtctac aatggggcgc gaaacgagat
     2701 gaggaattcg gttgaatccg caagaagaat gacaagtata cggcactttc cggcgaccca
     2761 gccgctgccc gactggcgca acttcggcgt catgctgcgc gtcctgctcg gcatcaacct
     2821 gctcgccgcc gtcgccgcgc tgatccagtc gccggacctg gcgagctggg gtgagcgctt
     2881 cgtcgccatg gcctcggtgg tcgaaccgtt gctgctgatc ggtctcgccg tcctgtcgct
     2941 gtgccgcgac ctgctctggc gcctgcgcct gcgcgtcggc caggcctgtg tgctggcctt
     3001 ggccggtggc ctggcggcgg tgctcttcgc ctatggacat tcgctgcacc tggtcgaggc
     3061 cgaggcggtc actcgcggca gcctgctcgc catgacggca accgggatgc tgctgctcta
     3121 tttcgaactg cgctcgcggg cgctgtcgcc ggcccaggcc gaggcgcggc tggcagcgtt
     3181 g
//
LOCUS       DAOSDX010000266         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10777_length_3175_cov_3.426603, whole genome shotgun sequence.
ACCESSION   DAOSDX010000266 DAOSDX010000000
VERSION     DAOSDX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10777_length_3175_cov_3.426603"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..967)
                     /locus_tag="PLN02_06895"
     CDS             complement(167..967)
                     /locus_tag="PLN02_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52090.1"
                     /translation="MFAAPPPPPQRAFYLACLLSIALHLLVLFVPRHEPAAGQRPPSR
                     LEARLQPRPASLPEAPASIAAPPPPAAARPAARRPVLSSKRPGATSAPTWSAAEKAEM
                     NDFLDELARAPRPTLAQRSRAMAREEGRRLAAQDEAEEALLELRPNAPPIHPFSLESY
                     LDGMIRRLNRSARYVSNDKRALGVQTAAIQFRLNPDGTLKSFVVLKAGDQGAEIAFIK
                     AIIERSLPFSPFPPDIDRAARSLGITICIRPGSGDGDFGFSRMQGSRC"
     gene            1202..2299
                     /gene="tssA"
                     /locus_tag="PLN02_06900"
     CDS             1202..2299
                     /gene="tssA"
                     /locus_tag="PLN02_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HOI52091.1"
                     /translation="MLRPLVGSAGPCGEDMIFSSEFDEIQEARRFDDPSISQGEWVTT
                     IKEADWPAVIRLGERLLTTKTKDLRLAAWMIEARAKTAGLDGLAEGYWLLAGLAENFW
                     AAIHPLPEDADDDVRVGVLDWLVNQSARLIREIPLTKSKKGQYSSIDREAARNVAKNV
                     ELNSSYAEEAARNGMLTMEVFEAALKDTPRAYFLTQLQSAERLQGAIKRLQEVVDPLL
                     GEQSPAFGASLEALQEIMHFFRRQAGGDLPVNQPEAGIISETEDKEAMFSMPGRMEPR
                     IGELAGEKQAGPIRSREHAIYLLQEIAGYFRRTEPHSPVAYLAQKAAKWGTMPLHQWL
                     RSVVKDDASLSHVEELLGVNAMLPDDTDSAS"
     gene            complement(2329..3096)
                     /locus_tag="PLN02_06905"
     CDS             complement(2329..3096)
                     /locus_tag="PLN02_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI52092.1"
                     /translation="MKFRTIAASLALLACLLPSAQANPPGAVIASGVVPDEAARAAIL
                     SRLREVFGAANIVDRLEVGGVVPPPNWADYMGKLLTENLKEVHKGQMQVTGTRVALKG
                     TVANEALRQQVVSKIAPALNPTYTVDNALVVAGPAAQEVLDRTLSNRIVEFESGSATL
                     TAKGIIILDEMVAAMKQIGMPKVQIIGHTDSSGNRQANVSLSLARANSVRDYLVSKGI
                     PAESLTAMGSGPDHPVTSNATAEGRARNRRIEFRQTG"
BASE COUNT          567 a          959 c         1058 g          591 t
ORIGIN      
        1 cagccagccg tggtggtcgc ggtagagcgt cgacagggaa tcggacgggg cagcgcaggc
       61 caagggaagc tccggaaagg aatgctggct ggcccgatgg gcgtggcttg cgttagtttt
      121 taagaatggt tctcattgtc tgatattgcc gcggcagcgt caatgctcag cagcggctgc
      181 cctgcatccg cgagaagccg aaatcgccgt caccactgcc cggccggatg cagatggtga
      241 tcccgagcga gcgcgcggcg cggtcgatgt ccggcggaaa gggtgaaaac ggcagcgagc
      301 gctcgatgat cgccttgatg aaggcgattt ccgcgccctg gtcgccggcc ttgagcacga
      361 cgaaactctt cagcgtgccg tccgggttga ggcggaactg gatcgccgcc gtctgcacgc
      421 cgagcgcgcg cttgtcgttg ctgacgtagc gggcgctgcg gttgagccgg cggatcatgc
      481 cgtcgagata gctttcgagg ctgaacggat ggatcggcgg cgcgttcggg cgcaattcga
      541 gcagcgcttc ctcggcctcg tcctgcgccg ccagccgccg gccttcctcg cgcgccatcg
      601 cccgcgagcg ctgggccagc gtcggccgcg gggcgcgcgc cagttcgtcg aggaaatcgt
      661 tcatctcggc cttttcggcg gcgctccagg tcggcgcgct ggttgcgccc ggccgcttgc
      721 tgctcaggac cgggcggcgc gctgccggcc tggcggcggc gggcggcggt ggagcggcga
      781 tgctggccgg ggcctcgggc aggctcgccg gacgcggctg cagcctggcc tcgagccgcg
      841 acggcgggcg ctgcccggcc gccggctcat ggcgcggcac gaacaggacc agcaggtgca
      901 gggcgatgga gagcaggcag gccaggtaaa aggcacgttg cggcggaggg ggtggagcgg
      961 cgaacatcgc gatcattgta gtcggaggtt tgacatcagg catgaggttg gcgcgctgag
     1021 cagatccctt ttgcacgcgg cagttaacat aatacaaatt atgcgcgttt tataaccgga
     1081 ttcgcgatgc gcttctgaca accgagtccc aatttattag aatggcatct ttgtatgaat
     1141 tagccatatg cagccatctc cttgggatcg cctgcatgtt aaaacctcag gagttagaaa
     1201 cttgttgcgt cccctggtag gtagcgccgg tccttgcggc gaagacatga tcttttcttc
     1261 cgagttcgac gagattcagg aagcccggcg ctttgacgat ccgtcgattt cccaaggcga
     1321 atgggtaaca acgatcaagg aggcggattg gccggcagtg attcgtctcg gcgaacgcct
     1381 gctcactacg aaaaccaagg acttgcggct ggcggcatgg atgattgagg cgcgtgccaa
     1441 gaccgccggt ctcgacggtc tggccgaagg ctattggcta ctggcggggt tggcggaaaa
     1501 tttctgggcg gcgatacatc cgctgccgga agacgccgac gacgatgtcc gggtcggcgt
     1561 gctcgactgg ctggtcaacc aatccgcccg cttgatccgc gaaattccgc tgaccaagtc
     1621 gaagaagggg caatattcca gcattgaccg ggaagccgcc cgcaacgtgg cgaagaatgt
     1681 cgaactcaac tcgtcctatg ccgaggaagc ggcgcgcaac ggcatgctga cgatggaagt
     1741 attcgaggct gcgctcaagg atacgccgcg cgcctacttc ctcacccaac tgcagtctgc
     1801 cgaacgcctg cagggcgcga tcaagcgctt gcaggaagtc gtcgatcctc tgctgggcga
     1861 gcaatcgcct gctttcggag cgagcctcga agcattgcag gaaatcatgc atttcttccg
     1921 gcgccaggcg ggtggcgatc ttcccgttaa ccagccggaa gccggaatca tttccgagac
     1981 ggaggacaag gaagccatgt tttcaatgcc aggcaggatg gagccaagaa tcggcgagct
     2041 tgccggtgaa aaacaggccg ggccgattcg ctcccgcgag cacgcgatct atctgctgca
     2101 ggaaatcgcc ggctatttcc gccgcaccga accgcacagc ccggttgcct atctggcgca
     2161 gaaggccgcc aaatggggaa cgatgccgct acaccagtgg ctgcgcagcg tcgtcaagga
     2221 cgacgcctcg ctgtctcacg tcgaggaatt gctcggcgtc aacgccatgt taccggacga
     2281 caccgacagc gcttcctgaa tacccggcgc tcttcgagcg agcgccgcct agccagtttg
     2341 ccggaactcg atgcggcggt tgcgtgccct gccctccgcc gtcgcgttgc tggtgaccgg
     2401 atgatccggc ccggaaccca tcgcagtcag cgattcggcc ggaatcccct tgctgaccag
     2461 gtagtcgcgc acgctgttgg cacgggccag gctgaggctg acattggcct ggcggttacc
     2521 ggagctgtcg gtgtggccga tgatctgcac cttgggcatg ccgatctgtt tcatcgctgc
     2581 caccatctca tcaaggatga tgattccttt ggcagtgagc gtcgccgacc cgctttcaaa
     2641 ctcgacgatg cggttcgaca gcgtccggtc gagcacttcc tgggcggccg gaccggcaac
     2701 caccagggcg ttgtcgacgg tataggtcgg attcagcgcg ggggcgatct tgctcaccac
     2761 ctgttgccgc aacgcctcgt tggcaaccgt gcctttcagc gcgacgcggg tgccggtgac
     2821 ctgcatctgc cccttgtgca cttccttgag gttttcggtc agcagcttgc ccatgtaatc
     2881 cgcccagttg ggcggcggca cgacgccgcc gacttccagc cggtcgacga tgttggcggc
     2941 accgaaaacc tcgcgtagcc ggctcagaat cgccgcccgc gcggcttcgt cggggaccac
     3001 gccgctggcg atgacggctc caggaggatt ggcctgggcc gaaggcagca ggcaggcaag
     3061 cagagccagg cttgccgcga tggttcggaa tttcatgctc attctcccag gtagacttcg
     3121 cggaacgtct gaatcgccag ttccagcgtg atggcgggct gttcgagata ggcgg
//
LOCUS       DAOSDX010000267         3164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10826_length_3164_cov_3.810550, whole genome shotgun sequence.
ACCESSION   DAOSDX010000267 DAOSDX010000000
VERSION     DAOSDX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10826_length_3164_cov_3.810550"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLN02_06910"
     CDS             <1..325
                     /locus_tag="PLN02_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOI52093.1"
                     /translation="TLIGERIVRAVDDERAPELAALVRHHHENFDGSGYPDGLRQTDI
                     PLTARVLALADNYDALATSRPYHPGRPHREIIDILAGPDAEKFDPDLLHAFCHVIEHS
                     PRRAG"
     gene            complement(332..1429)
                     /gene="mnmA"
                     /locus_tag="PLN02_06915"
     CDS             complement(332..1429)
                     /gene="mnmA"
                     /locus_tag="PLN02_06915"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOI52094.1"
                     /translation="MSKGTVVVGLSGGVDSSVAALLLKRQGWKVIGLFMKNWEDDDND
                     EYCSSRQDLVDVMSVADVIGIDVEVVNFAAEYRERVFAEFLREYQAGRTPNPDILCNS
                     EIKFKAFLDHALQLGAEKIATGHYAGVREFNGEYQLLKAEDGTKDQSYFLYRLNQAQL
                     SKTLFPLADIYKREVRKIAEAEGLHVAAKKDSTGICFIGERPFKDFLSRYLPPKQGEI
                     RRLDDGKVLGSHDGLMYHTIGQRKGLHIGGLKEKGQAGGGDHDAWFVAGKDMEKNVLY
                     VVQGHDHPALLRDDLIAEQLSWIAGRPPHPHWVYTAKPRYRTADQPCEVERVDGVSCE
                     IRFAEPQWALTPGQSVVLYESRVCLGGGVIR"
     gene            complement(1422..1970)
                     /locus_tag="PLN02_06920"
     CDS             complement(1422..1970)
                     /locus_tag="PLN02_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52095.1"
                     /translation="MRQAALAAAFFLLSGAATADEMVAVCYGYGCSAQAEIRYSTGQQ
                     GEVRRLLAYAGDPASERIILGKVVGRLYRWAGEQSPVREDRGGNYADSDAPGKMDCID
                     HSTSTTRLLEMLQRLGYLRWHRVLAAEPRYLLGVIANHWSAVIEEKGGGRYVVDSWFV
                     NNGEPAVILPLEEWKKGAGPDV"
     gene            complement(1970..2416)
                     /locus_tag="PLN02_06925"
     CDS             complement(1970..2416)
                     /locus_tag="PLN02_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOI52096.1"
                     /translation="MIWKPNVTVAAVVYRDGRFLLVEEETETGLAFNQPAGHLEQGEA
                     LLDAVVREALEETAYDFKPTHLVGIYNWPHPTKDLTYLRFAFAGELRGHDPARKLDDG
                     IVGARWLTLDEVRATQARHRSPLILRCCEDYLAGRRYPLDLLVHYG"
     gene            2507..>3164
                     /locus_tag="PLN02_06930"
     CDS             2507..>3164
                     /locus_tag="PLN02_06930"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOI52097.1"
                     /translation="MSMSDRDGFIWQDGKLVPWREATTHVLTHSLHYGMGVFEGVRAY
                     KTESGTAIFRLKEHTERLFRSAHIFQMQMPYSQEELNEAQKEVIRANKLESGYLRPLA
                     FYGSEKMGVSPKGAKVHVIIAAWPWGAYLGEEGMQRGIRIKTSSYTRHHVNITMVRAK
                     ASGNYMNSILANNEATTDGYDEALLLDPEGYACEGAGENLFIVKKGKLYTPDLTACLE
                     G"
BASE COUNT          574 a         1078 c          955 g          557 t
ORIGIN      
        1 cacgctgatc ggcgaacgca ttgtccgcgc cgtcgatgac gaacgggcgc cggaactggc
       61 ggcgctggtc cgccaccatc atgagaattt cgacggctcg ggctacccgg acggcctgcg
      121 ccaaaccgac attccgctga cggcgcgcgt gctggcgcta gccgacaact acgatgcact
      181 ggcgaccagc cgcccctatc acccggggcg gccgcaccgg gaaatcatcg acatcctggc
      241 cggcccggac gccgagaagt tcgaccccga cctgctgcac gccttctgcc acgtcatcga
      301 gcattcgccc cgccgcgctg gctgaggcgc ttcagcggat gacgccgccg cccaggcaga
      361 cccggctttc gtagagaacc accgactggc cgggggtgag cgcccattgc ggctcggcga
      421 agcggatttc gcaggacacg ccgtcgacgc gctcgacctc gcagggttga tcggccgtcc
      481 gatagcgcgg cttggcggtg tacacccagt gcgggtgcgg tggccggccg gcaatccagc
      541 tcagttgctc ggcgatcagg tcgtcgcgca gcagcgccgg atggtcgtgg ccctggacga
      601 cgtagagcac gttcttttcc atgtccttgc cagcgacgaa ccaggcgtcg tggtcgccac
      661 caccggcctg ccccttctcc ttcaggccgc cgatgtgcag ccccttgcgc tggccgatgg
      721 tgtggtacat caggccgtcg tgactaccca acaccttgcc gtcgtcgaga cggcggattt
      781 cgccctgctt gggcggcagg tagcggctga gaaagtcctt gaacggccgt tcgccgatga
      841 aacagatacc ggtcgaatcc ttcttggcag cgacgtgcaa gccctcggcc tcggcgattt
      901 tcctgacctc gcgcttgtag atgtcggcca gcgggaacag cgttttcgag agctgcgcct
      961 ggttcaggcg gtagaggaaa tagctctggt ccttggtccc gtcctcggct ttcagcaact
     1021 ggtattcgcc attgaattcg cgcacaccgg cgtagtggcc ggtggcgatc ttctcggcgc
     1081 cgagctgcag ggcgtggtcg aggaaggcct tgaacttgat ctccgagttg cacaggatgt
     1141 ccgggttcgg cgtccgcccg gcctggtatt cgcgcaggaa ctcggcgaac acgcgctcgc
     1201 ggtactcggc ggcgaaattg accacctcga cgtcgatgcc gatgacgtcg gcgacgctca
     1261 tcacgtcaac cagatcctgg cgcgacgagc agtattcgtc gttgtcgtcg tcttcccagt
     1321 tcttcatgaa caggccgatc accttccagc cctggcgctt caacaacagc gccgcgaccg
     1381 acgaatcgac gccgccggac aatcccacta ccacggttcc cttagacatc gggcccagcc
     1441 cctttcttcc attcttccag cggcaggatc accgccggtt ctccgttgtt cacaaaccag
     1501 ctatcgacga cgtagcggcc gccgcccttt tcctcgatca ccgccgacca gtggttggca
     1561 atcacgccca gcaggtaacg cggctccgcc gccagcaccc gatgccagcg caggtagccg
     1621 aggcgttgca gcatttccag caggcgcgtc gtcgaggtcg aatggtcgat gcaatccatc
     1681 ttccccggcg cgtcgctgtc ggcatagttg cctccccggt cttcacgcac cggcgactgt
     1741 tcgccagccc agcgatagag gcggccgacg accttgccca ggatgatgcg ctcgctcgcc
     1801 ggatcgccgg cgtacgccag caggcgccgg acttccccct gttgccctgt ggaatagcgg
     1861 atctcggctt gcgcgctgca gccataaccg taacagacgg ccaccatctc gtccgccgtc
     1921 gccgcccccg agagcagaaa aaaggccgcg gcgagagcag cctgtcgcat caaccgtaat
     1981 gcaccagcag gtcgagcggg taacgccgcc cggccagata gtcctcgcag cagcgcagga
     2041 tcagcggact gcgatggcgc gcctgcgtcg cccggacttc gtcgagcgtc agccagcggg
     2101 cgccaacgat gccgtcgtcg agcttgcgcg cgggatcgtg accgcgcagt tcgccggcga
     2161 aggcgaagcg caggtaagtc agatccttgg tcggatgcgg ccagttgtag atgccgacga
     2221 ggtgcgtcgg cttgaaatcg taggcggttt cctcaagcgc ctcgcgcacc acggcgtcaa
     2281 gcagcgcctc gccctgctcg agatggccgg ccggctgatt gaaggcgagc ccggtctcgg
     2341 tttcttcctc gaccagcaaa aacctgccat cgcgatagac gacggcggca acggtcacgt
     2401 tcggcttcca gatcatgcaa ggcttcctgc gtcaaaagca ttattttagc cgctgatttc
     2461 ggctaaaatt tcatgctttc aagagtgcat cacggagaat tccaccatgt ccatgtcgga
     2521 tcgcgacggt ttcatttggc aggatggcaa gctcgtgccc tggcgcgaag cgaccaccca
     2581 cgtcctcacc cattccctgc actacggcat gggcgtcttc gagggcgtgc gcgcctacaa
     2641 gaccgaatcc ggcaccgcga tcttccgcct caaggaacat accgagcgcc tgttccgctc
     2701 ggcgcacatc ttccagatgc agatgccgta ctcgcaggaa gagctgaacg aagcgcagaa
     2761 ggaagtcatc cgcgccaaca agctggaatc cggctacctg cgcccgctcg ccttctacgg
     2821 ctcggaaaag atgggcgtct cgccgaaggg cgccaaggtg cacgtgatca tcgccgcctg
     2881 gccctggggc gcctacctcg gcgaggaagg catgcagcgc ggcatccgca tcaagacctc
     2941 gtcctacacc cgccaccatg tgaatatcac catggtccgc gccaaggcct cgggtaacta
     3001 catgaactcg atcctcgcca acaacgaggc gaccaccgac ggctacgacg aagccctgct
     3061 cctcgacccg gaaggctatg cctgcgaagg cgccggcgag aacctgttca tcgtcaagaa
     3121 gggcaagctg tacacgccgg acctcaccgc ctgcctggaa ggca
//
LOCUS       DAOSDX010000268         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10847_length_3159_cov_2.803802, whole genome shotgun sequence.
ACCESSION   DAOSDX010000268 DAOSDX010000000
VERSION     DAOSDX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10847_length_3159_cov_2.803802"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2408)
                     /locus_tag="PLN02_06935"
     CDS             complement(<1..2408)
                     /locus_tag="PLN02_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HOI52098.1"
                     /translation="MSLALIVILPFLGAALPALLIRAGRNACTTGTLATSLLAFLLLL
                     THAPAVFRGEVLQARWEWIPQLGLNVNFFLDGLGFFFAGLILGIGLLIIVYARFYLSK
                     EDPMGSFYSYLLLFQGAMVGVVLSDNILLLVIFWELTSLSSFLLIGYWKHLPAGRQGA
                     RMALTVTGAGGLAMIAGMLILGNIAGSYDLSTILQHKEAIQASPLYLPALLLILAGCF
                     TKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGLFLMARLWPVLAGTDEWFYLVAS
                     TGLVTMLIGAWIALFKDDLKGLLAYSTVSHLGLVTFLLGLGSPLAAVAAVFHILNHAT
                     FKAALFMNAGIVDHETGTRDIRQLGGLMTLMPIAGTLATIAAASMAGLPLLNGFLSKE
                     MMLEQAAHTSWLDLPWLVPLLATLAATFSVAYSFRYIVHVYFGKPAGETPRQPHDPPF
                     GMWGPPALLVVLVVLIGLFPASVAGPLVATVAGAVIGGPLPDYHLAIWHGLTPALGMS
                     AIAVASGLGLLAGYARLRGLWSAGPHPEAKAIFDAVVGTAVRLAQALTHGLHNGSLQR
                     HLAFAIGSAVVVGFTAFAMASHAPGARPLLPATPVAIVAWLLLLGATGALLALHRRRL
                     LSLVVVGVIGMVVSLAFVYLSAPDLALTQISVEVVTVILMLLALNFLPKETPLESSGG
                     RRLRDGLLAALAGIGVGGAAWAMTTRDAATLSGYYLEESVPGGGGTNVVNVILVDFRG
                     FDTFGEIIVLGIAALTIYALLDGATRGAVGRRLAAWAPDQARAADRHPLLLVVATRVM
                     LPLAL"
     gene            complement(2578..3108)
                     /locus_tag="PLN02_06940"
     CDS             complement(2578..3108)
                     /locus_tag="PLN02_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52099.1"
                     /translation="MTAPILIIDDDPTFNAILIRTLGRRQQTAVGAHSSEEALQLAQT
                     HQPSRIVLDLNLAGVSGLSLIPQLLDINPQARIVVLTGYGSIATAVEAIKRGATQYLA
                     KPVEIEAILSAFDDADPGDSDSTPDEPLSVDRLEWEHIQRVLKENDGNVSATARALKM
                     HRRTLQRKLAKHPVRA"
BASE COUNT          559 a          947 c         1210 g          443 t
ORIGIN      
        1 agcgccagcg gcagcatgac gcgggtggcg accacgagca gcagcgggtg acggtcggcg
       61 gcccgcgcct ggtcgggtgc ccaggctgcc agcctgcggc cgaccgcgcc gcgcgtggcg
      121 ccgtcgagca gggcgtagat ggtcagcgcg gcgatgccca ggacgatgat ctcgccgaag
      181 gtgtcgaagc cgcggaaatc gaccaggatg acattgacca cgttggtgcc gccgccgccg
      241 ggcaccgatt cttccagata gtagccggac agcgtcgccg cgtcgcgcgt cgtcatcgcc
      301 caggcggcac cgccgacgcc gatgccggcc agcgcggcga gcagtccgtc gcgcaggcgt
      361 cggccgccgg aactttcgag cggcgtctcc ttgggcagga aattgagcgc cagcagcatc
      421 aggatgacgg tgacgacctc gaccgaaatc tgcgtcagcg ccaggtccgg cgccgacaga
      481 tagacgaagg cgagtgagac gaccatgccg atgacgccga ccacgaccag cgacagcagg
      541 cgtcgccggt gcagggcgag cagcgcgccg gtggcgccga gcagcagcag ccaggcgacg
      601 atggccaccg gcgtcgccgg cagcaggggg cgcgcgcccg gcgcatggct ggccatggcg
      661 aaggcggtga agccgacgac gacggcgctg ccaatggcaa acgccaggtg gcgttgcagc
      721 gagccgttgt gcaggccatg cgtgagcgcc tgggcaagac gcacggcggt gccgacgacg
      781 gcgtcgaaga tggccttggc ttccggatgc gggccggccg accacagacc acgcaggcgg
      841 gcgtagccgg cgagcaggcc gagaccgctg gcgacggcga tcgcgctcat gccgagcgcc
      901 ggcgtcagac cgtgccagat cgccagatgg tagtcgggca gcgggccgcc gatcaccgcg
      961 ccggcaacgg tggcgaccag cgggccggcg acgctcgccg gaaagaggcc gatcaggacg
     1021 accagcacga ccagcagcgc cggcggcccc cacatgccga agggcgggtc gtgcggctgg
     1081 cgcggcgttt cgccggccgg tttgccgaag tagacatgga cgatgtagcg gaacgagtag
     1141 gcgaccgaga aggtcgccgc cagcgtcgct agcagcggca ccagccaggg caggtcgagc
     1201 cagctggtgt gcgccgcctg ttcgagcatc atttccttcg acaggaaacc gttcaacagc
     1261 ggcaggccgg ccatcgacgc ggcggcgatg gtggccagcg tgccggcgat cggcatcagc
     1321 gtcatcagcc cgccgagctg gcggatgtcg cgggtgccgg tttcgtggtc gacgatgccg
     1381 gcgttcatga acagcgccgc cttgaaagtg gcgtgattga ggatgtggaa gacggcggcg
     1441 acggcggcca gcggcgaacc gaggccgagc aggaaggtga cgaggccgag gtggctgacc
     1501 gtcgaatagg cgagcaggcc tttcaggtcg tccttgaaca gcgcgatcca ggcgccgatc
     1561 agcatggtga cgaggccggt gctggcgacc aggtagaacc attcgtcagt gccggcgagc
     1621 accggccaga gccgcgccat caggaacagg ccggccttga ccatggtcgc cgagtgcagg
     1681 taggcggaga ccggcgtcgg cgccgccatc gcgtgcggca gccagaaatg gaaggggaac
     1741 tgcgcgctct tggtgaagca gccggcgagg atcagcagca gcgccggcag gtagagcggc
     1801 gaggcctgga tcgcttcctt gtgctggagg atcgtcgaca gatcgtagga accggcgatg
     1861 ttgccgagga tcagcatgcc ggcgatcatc gccagaccgc cggcgccggt cacggtgagc
     1921 gccatgcgcg cgccctggcg gccggccggc aggtgtttcc agtaaccgat gaggaggaag
     1981 gacgagaggc tggtcagttc ccagaagatc accagcagca ggatgttgtc ggacagcacg
     2041 acgccgacca tggcgccctg gaagagcagc aggtagctgt agaagctgcc catcgggtct
     2101 tccttgctca ggtagaaacg ggcgtagacg atgatcagca ggccgatacc gaggatcagg
     2161 ccggcgaaga agaagcccag gccgtcgagg aagaaattga cgttgaggcc cagttgcgga
     2221 atccattccc agcgcgcctg cagcacctcg ccacggaaaa cggccggggc gtgcgtcagc
     2281 agcagcagga aggcgagcag gctggtggcc agggtgccgg tggtgcaggc gttgcggccg
     2341 gcgcggatga gcagcgccgg gagtgcggcg ccgagaaagg ggaggatgac gatcagcgcc
     2401 aggctcatgg gctctctttc cggatgtcac ggtgaacaaa tgctgcggtg gcagtgcggg
     2461 agaccacgat caagctgtgg agcggccagt ataaaggccg ctttacggat tcaccaaggc
     2521 ggggcgggga aaagaagctt cggtttttcc ggaacgaccg tccggggctt tccggtttca
     2581 ggcgcggacc gggtgcttgg caagcttgcg ttgcagcgtg cggcgatgca tcttcagggc
     2641 gcgggcggtg gccgagacgt tgccgtcgtt ttccttgagc acgcgctgga tgtgttccca
     2701 ttccaggcgg tcgaccgaca gcggctcgtc gggcgtgcta tcgctgtcgc cggggtcggc
     2761 gtcgtcgaag gcgctgagga tggcctcgat ttcgaccggc ttggcgaggt actgggtggc
     2821 gccgcgcttg atcgcttcga ccgcagtggc gatgctgccg taaccggtga ggacgacgat
     2881 gcgcgcctgc ggattgatgt cgagcaattg cgggatcagc gacagcccgg aaacgccggc
     2941 caggttgagg tcgaggacga tgcgcgaggg ctggtgcgtc tgcgccagtt gcagcgcttc
     3001 ctcgctgctg tgggcgccga ccgcggtctg ctggcggcgg cccagggtgc ggatcaggat
     3061 cgcgttgaag gtcgggtcgt cgtcgatgat caggatcggt gcggtcattt cttctcgatg
     3121 ggtagcagga tgcgggcgcg ggcgccgccg tcgtcgggg
//
LOCUS       DAOSDX010000269         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10866_length_3156_cov_4.416962, whole genome shotgun sequence.
ACCESSION   DAOSDX010000269 DAOSDX010000000
VERSION     DAOSDX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10866_length_3156_cov_4.416962"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLN02_06945"
     CDS             complement(<1..964)
                     /locus_tag="PLN02_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOI52100.1"
                     /translation="MANQPLFRRAALDAQKPKSCGEILLVRPLSYRLLSLAALVCTLA
                     IVALFTWGSYTKRSSVTGQLVPSAGLLRIYPPQTGIVGKKLVAEGQSVKAGDTLYLIS
                     SERQSSTLGSVFASVSEQLGARQESLTRELERTRQLQREEQEGLTRQVAALRSELEKI
                     DSLLEGQRARVALAEETSGRYQSLLEQDYISREQRQQKREELLDQQTRLKSVEREQIA
                     LRRELGTRQESLDALRFKHANQLAQIERVISSTSQELSESEGRRMLAITAPIDGTATA
                     VVAEVGQAVDGRRPLLSIVPAGSQLEAQLYAPSRAVGFVRPGAQV"
     gene            complement(1131..1346)
                     /locus_tag="PLN02_06950"
     CDS             complement(1131..1346)
                     /locus_tag="PLN02_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52101.1"
                     /translation="MAIQELSKTEIAAVSGGALDLGGLLGGILNPVLGLVGGILTPVV
                     SLVSGLVGTVTGLLGGLLGGLFGGSCQ"
     gene            complement(1388..1594)
                     /locus_tag="PLN02_06955"
     CDS             complement(1388..1594)
                     /locus_tag="PLN02_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52102.1"
                     /translation="MAIQELSKTEIAAVSGGALDLGGLLGGILNPVLGLVGGLLTPVL
                     GLVSGLLGTVTGLLGGLLGGLLGN"
     gene            complement(1645..1857)
                     /locus_tag="PLN02_06960"
     CDS             complement(1645..1857)
                     /locus_tag="PLN02_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52103.1"
                     /translation="MAIQELGKTEIEAVSGGTLTLDLHADVGSLLGGLLGPVFSLVGG
                     LVAPTFSLVGGLLGAVTGLLGGLLSR"
     gene            2199..>3156
                     /locus_tag="PLN02_06965"
     CDS             2199..>3156
                     /locus_tag="PLN02_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI52104.1"
                     /translation="MITSLLLLVLFVLFCCVVFWRAARIERVLQRLGDGDPAARTGLA
                     PWAPLGRLAQRVDWAAERLAEERRRLADSEGRLRIALQGCDIGVWDWQLDSGRIYYSP
                     RWKTLLGFAEDETPGHAETWLTRVHPDDLARVTAALDEHLSGHSEIFLCEHRLRHKRG
                     DYLWVQQSGVASRDDSGRVLRMIGALADVSAHKAAQAELLSSREAYRSVVEGVTQVIL
                     RSDVAGRLSFLNPAWQELTGHSIDEALGKTLASFVHDDERHSVSAFFAALADGAAGDL
                     SCELRLRCRDDAWRWFALYLRATPAAGAASAAGVLSDIAAQKA"
BASE COUNT          519 a          963 c         1108 g          566 t
ORIGIN      
        1 gcacctgggc gcccgggcgg acgaagccga ccgcccggct cggcgcgtac aactgcgctt
       61 cgagctggct gccggcgggg acgatgctga gcaacgggcg ccggccatcg accgcctggc
      121 cgacttcggc gaccaccgcg gtggcggtgc cgtcgatcgg cgccgtgatc gccagcatgc
      181 ggcgcccctc gctttcgctc agctcctggc tggtcgagga aatgacccgt tcgatctggg
      241 ccagttggtt ggcgtgcttg aagcgcaagg cgtcgaggct ttcctggcgg gtgccgagtt
      301 cgcggcgcag ggcaatctgc tcgcgctcga cgcttttcag ccgggtctgc tggtcgagca
      361 gttcctcgcg cttctgctgg cgctgttcgc gcgagatgta gtcctgctcc agcagcgact
      421 ggtagcggcc gctggtttcc tcggccaggg cgacgcgggc gcgctggcct tcgagcaggc
      481 tgtcgatctt ctccagttcg ctgcgcagcg ccgccacctg ccgggtcagg ccttcctgtt
      541 cctcgcgctg cagttgccgg gtgcgttcga gttcgcgggt cagcgattcc tggcgggcgc
      601 cgagctgctc gctgacactg gcaaagaccg agcccagggt gctgctctgg cgctcgctgg
      661 aaatcaggta aagggtgtcg ccggccttga ccgactggcc ttcggcgacc agcttcttgc
      721 cgacgatgcc ggtctgcggc ggatagatgc gcaacaggcc ggcgcttggt accagctggc
      781 cggtgacgct gctgcgcttg gtatagctgc cccaggtgaa caaggcgacg atggccagcg
      841 tgcagaccag ggcggccagg ctcaacaggc ggtagctgag tgggcggacg agcaggattt
      901 cgccgcagga cttgggtttt tgcgcgtcga gcgcggcgcg tcggaacagg ggctggttgg
      961 ccatgctgaa tgaattcggt tggcgctgga tgctgttggg atttttcggg acggccgaaa
     1021 tcgaggccac tgaaggcgcc gggaattggt caggaaagct gtcgggagag cagacgcgag
     1081 gcgggtgagg gcaggtcgtt cagggatccg cctcgcgctg cccaggcgga ttactggcag
     1141 gaaccgccga acaggccgcc aagcagaccg ccgagcaggc cggtgacggt gccgacgagg
     1201 ccgctgacca ggctgaccac cggcgtcaga atgccgccga ccaggccgag gaccgggttc
     1261 aggatgccgc cgagcaggcc gccgagatcc agagcgccgc cggaaacggc tgcgatttca
     1321 gtcttgctga gttcttgaat tgccatgttg ttactccttg gattttcgtc aggtcgaaat
     1381 gacccgatca gttgcccaac aggccgccga gcaggccgcc cagcagaccg gtgacggtac
     1441 cgagcaggcc gctgaccagg ccgagaaccg gggtcagcag gccgccgacc aggccgagga
     1501 ccggattcag gatgccgccg agcaggccgc cgagatccag ggcgccaccg gaaacggcag
     1561 cgatttcagt cttgctgagt tcttgaattg ccacgttgct actccttgtg ttgaacgaaa
     1621 actggtgaag gtgaattgtt tttcttagcg gctcagcaga ccgccgagca ggccggtgac
     1681 agcgccgagc aggccgccga ccaggctgaa ggtcggagcg accagaccgc cgacgaggct
     1741 gaagaccgga cccaggaggc cgcccaggag gctgccgacg tcggcatgca ggtccagggt
     1801 cagggtgccg ccggagacgg cttcgatttc ggtcttgcca agttcttgaa ttgccatgtt
     1861 tgttactcct tgactgattg aaaaacgcca caccgggtgg catgcattgc ccgtagacaa
     1921 ttctgttttg cagttatgca tattcgagtt tcaaaaacag tacaaacaat gcacaatgca
     1981 aattgttgcg acatgataat caaattatgc ggaattgcaa tcttgctcta ggggttttcg
     2041 taaaatcgcg cagcgtaagc ggtaaaagtg aaaattcgtc gccgcgacga tggctgcctt
     2101 tcgtccgtgg cgcgctgtcg gcctgcgcca ttcatgccgc tgccgcgctg gccgaggagg
     2161 aagtgcacga agctgcggct ttgtcttatc ctgatgcaat gatcaccagc ctcctgctcc
     2221 tcgtcctgtt cgttcttttc tgctgcgtgg tgttctggcg ggcggccagg atcgagcggg
     2281 tgctacagcg gctcggcgac ggcgatccgg cggcgcgcac cgggctggcg ccctgggcgc
     2341 cgctcggtcg tctggcgcag cgcgtcgatt gggcggccga gcgcctggcc gaggaaaggc
     2401 ggcgcctggc cgacagcgag ggacgcctgc gcatcgccct gcagggctgc gacatcgggg
     2461 tctgggactg gcagctcgac agcggccgca tctattactc gccgcgctgg aaaaccctgc
     2521 ttggttttgc cgaggacgag acgccgggac acgccgagac ctggctgacg cgggtccatc
     2581 ccgacgatct ggcgcgcgtc acggcggcgc tcgacgaaca cctgtcgggg cacagtgaaa
     2641 tcttcctctg cgagcaccgg ctgcggcaca agcgcggcga ctacctgtgg gtgcagcaga
     2701 gcggcgtcgc cagccgcgac gatagcggcc gggtgctgcg catgatcggc gcgctggccg
     2761 acgtttcggc gcacaaggcg gcacaggccg aactgctgag cagccgggaa gcctaccgct
     2821 cggtggtgga aggggtgacg caggtgatcc tgcgcagcga tgtcgccggg cggctgagtt
     2881 ttctcaaccc ggcctggcag gaactgaccg ggcacagcat cgacgaagcg ctcggcaaga
     2941 cgctggccag cttcgtgcac gacgatgagc gtcacagtgt ctcggctttc tttgccgcgc
     3001 tggccgatgg ggcggccggc gatctttcct gcgaattgcg cctgcgctgc cgcgacgatg
     3061 cctggcgttg gttcgccttg tacctgcggg ccacgccggc ggccggcgcg gcgtcggccg
     3121 ccggcgtgct gagcgacatc gcggcgcaga aggcga
//
LOCUS       DAOSDX010000270         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10874_length_3155_cov_2.646129, whole genome shotgun sequence.
ACCESSION   DAOSDX010000270 DAOSDX010000000
VERSION     DAOSDX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10874_length_3155_cov_2.646129"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLN02_06970"
     CDS             <1..594
                     /locus_tag="PLN02_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOI52105.1"
                     /translation="PGTKVKGKVTSITDFGIFIGLPGGIDGLVHLSDLSWSATGEEAI
                     RNFKKGDEVEAVVLGIDVEKERISLGIKQLDGDPYANFIATHEKNSIVRGIVKTVDAR
                     GAVVTLDGENEAYLRASEFSRERTEDLSQHLKVGDEIEAMIINVDRKTRGINLSIKAK
                     DQAEQHEAMQKFSADTSAATGTTNLGALLKAKLNQQG"
     gene            606..890
                     /locus_tag="PLN02_06975"
     CDS             606..890
                     /locus_tag="PLN02_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOI52106.1"
                     /translation="MTKSELIVRLAERFPQLVAKDADFAVKMILDAMSEALVRGDRIE
                     IRGFGSFALNYRPPRVGRNPKSGDKVSVPAKWVPHFKAGKELRERVDQSI"
     gene            958..1239
                     /locus_tag="PLN02_06980"
     CDS             958..1239
                     /locus_tag="PLN02_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOI52107.1"
                     /translation="MTALTWFLRFIIFAFLVLFAVQNTAPVTLSLALDYYWQAPLVIV
                     LLAFFGGGALLGVLSLVGTIFRQRREIARLKRALLKAEQPPLPDLPLQQ"
     gene            1236..2408
                     /gene="lapB"
                     /locus_tag="PLN02_06985"
     CDS             1236..2408
                     /gene="lapB"
                     /locus_tag="PLN02_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HOI52108.1"
                     /translation="MNELFEYWQLLLVPVFFAMGWAAARVDMRQVVHESRALPRSYFK
                     GLNFLLNEQPDKAIDSFLEVAKVDSQTVELHFALGNLFRRRGETERAIRMHQNLLELP
                     EVEDKHRLQAMVELGQDFLKAGLLDRAEDIFNKLLGTAREADAKRSLLEIYQVGKEWQ
                     KAIEIARELPDVATHKEIAEYYCELAAGEIMRSRHDSARQYLEVAIQENRKCVRASIL
                     LGDLFAQEGKAAEAIDAWQAIEQQDPAYLSLVAQRLLDAYRKLSRSDAGVALLRGYLE
                     HYPSIDLLEVVYQLVLENEGNEAAYRLVRAELQRNPTLVGLERLMAARLPLASPEVRS
                     DLELGKSIVQGYTRRLSRYRCNNCGFKARQFYWRCPACGGWETSPPRRSEEFDQSL"
     gene            2416..>3155
                     /locus_tag="PLN02_06990"
     CDS             2416..>3155
                     /locus_tag="PLN02_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOI52109.1"
                     /translation="MLEKLSQVRLLVVGDVMLDRYWFGEVSRISPEAPVPVVKVERME
                     ERLGGAANVARNAASLGARTTLLSVVGDDDAGRALSRLLAESEIAADLKIDRQIDTTV
                     KLRVIGRQQQLLRIDFETAPSHEVLDAKLAEYERRLADCDIVILSDYGKGGLTHITEM
                     IRLARLAAKPVLVDPKGDEWEKYAGATVITPNRSELRDVVGRWSSEQMLFDKAEKLRG
                     ELGLEALLVTRSEEGMTLFADGQTFHQAA"
BASE COUNT          591 a          985 c          984 g          595 t
ORIGIN      
        1 ccgggcacca aggtgaaggg caaggtcacc tcgatcaccg acttcggcat cttcatcggc
       61 ctgcccggtg gcatcgacgg tctggttcac ctgtccgacc tgtcctggtc ggcgaccggc
      121 gaagaagcga tccgcaactt caagaagggt gacgaagttg aagccgtggt tctcggcatc
      181 gacgtcgaga aggaacgcat ctccctgggc atcaagcagc tcgacggtga tccgtacgcc
      241 aactttatcg ccacgcacga gaagaacagc atcgtccgcg gcatcgtcaa gacggtcgac
      301 gcccgtggcg ccgtggtcac gctggatggc gaaaacgaag cctacctgcg cgcttccgag
      361 ttctcccgcg agcgtaccga agacctgtcg caacacctga aggtcggtga cgagatcgaa
      421 gcgatgatca tcaacgtcga tcgcaagacc cgcggcatca acctgtcgat caaggccaag
      481 gatcaagccg aacagcacga agcgatgcag aagttctctg ccgacacttc cgctgccacc
      541 ggtacgacca acctgggtgc gctgctcaag gccaagctga accagcaagg ctgataggct
      601 gcgacatgac caaatccgag ctcatcgtcc ggctggcgga gcgttttccc cagttggtgg
      661 cgaaggatgc cgatttcgca gtcaagatga ttctcgatgc gatgtccgaa gcgctggtgc
      721 gcggagatcg catcgagatc cgcggatttg gcagcttcgc gctcaactac cggccgccgc
      781 gcgttggacg taacccgaaa tccggggaca aggtcagcgt gccggcgaag tgggttcccc
      841 acttcaaggc cggcaaggaa ctgcgcgagc gggtcgatca gtcgatctga agccgggctt
      901 ggcccaatgt ggtagattcg gggcagtgag tttcactgcc ccttttcatt gaatgtcatg
      961 acagccctga cctggttcct gcgtttcatc atcttcgctt tcctggtgct ctttgccgtc
     1021 cagaacacgg caccggtcac cttgagtctg gccctcgatt actactggca ggcaccgctg
     1081 gtcatcgtcc tgctcgcctt tttcggtggt ggcgccctgc ttggtgtgct ctccctggtt
     1141 ggcacgatat ttcgtcagcg acgcgaaatc gcccgtctca aacgcgccct gctcaaggcc
     1201 gagcagccac cgctgccgga tttgccgctg caacaatgaa tgaactgttc gaatactggc
     1261 agctcctgct ggtcccggtg tttttcgcca tgggctgggc tgccgcccgt gtggacatgc
     1321 ggcaggttgt ccatgaatcg cgggccttgc cgcgttccta tttcaagggc ctgaatttcc
     1381 tgctcaacga gcagccggac aaggccatcg attccttcct cgaagtcgcc aaggtcgatt
     1441 cgcagaccgt cgagctgcat tttgcgctcg gcaacctgtt tcgccggcgc ggcgagaccg
     1501 agcgggcgat ccgcatgcac cagaacctgc tggagctgcc ggaagtcgag gacaagcatc
     1561 gcttgcaggc gatggtcgaa ctcggtcagg atttcctgaa ggctggcctg ctcgaccgcg
     1621 ccgaagacat tttcaacaag ctgctgggta ccgcccgcga ggccgatgcc aagcgctcgc
     1681 tgctcgagat ctaccaggtc ggcaaggaat ggcagaaggc gattgagatc gcccgcgaac
     1741 tgcccgacgt cgcgacgcac aaggaaatcg ccgagtacta ctgcgagctg gctgccggtg
     1801 aaatcatgcg ttcgcgccac gattcggcgc gccagtatct cgaggtggcg atccaggaaa
     1861 accgcaagtg cgtgcgcgcc agcatcctgc tcggcgacct gtttgcccag gagggcaagg
     1921 cggcggaagc gatcgacgcc tggcaggcga tcgaacagca ggatcccgcc tacctgtcgc
     1981 tggtcgctca gcgcctgctt gacgcctacc gcaagctgtc ccgctccgac gccggggtgg
     2041 cgctgctgcg cggctatctc gaacattacc cgtcgatcga cctgctcgaa gtcgtgtacc
     2101 agctggtgct ggaaaacgag ggcaacgagg cggcctatcg cctggtgcgc gccgaactgc
     2161 agcgtaaccc gacgctggtt ggcctggagc gactgatggc ggcacgtcta cccttggcgt
     2221 cgccggaagt gcgcagcgat ctggaactgg gcaagagcat cgtccagggc tatacccggc
     2281 gcctgtcgcg ctatcggtgc aataattgcg gcttcaaggc gcgtcagttc tactggcgct
     2341 gcccggcctg cggcggctgg gagaccagcc cgccgcggcg cagcgaggaa ttcgatcaat
     2401 ccctgtagga cttcgatgct ggaaaaactc tcgcaggtgc gcctgctcgt cgtcggcgac
     2461 gtcatgctgg accgctactg gttcggcgag gtcagccgga tttcgccgga agcgccggtg
     2521 ccggtggtca aggtcgagcg catggaagag cgcctcggcg gtgctgccaa cgttgcccgc
     2581 aacgccgctt cgcttggggc gcggacgacc ctgctctcgg tggttggcga cgacgatgct
     2641 ggtcgggcac tctcccgtct gctcgccgag agcgagatcg ctgccgatct gaagatcgat
     2701 cgccagatcg acaccacggt caagctgcgc gtcatcggtc gccagcagca gttgttgcgc
     2761 atcgatttcg aaaccgcgcc gtcgcacgaa gtgctcgacg ccaagctcgc cgagtacgaa
     2821 cgccggctcg ccgattgcga catcgtcatc ctgtccgact acggcaaggg tgggctgacc
     2881 catatcaccg agatgatccg tctcgctcgc cttgccgcca agccggtgct ggtcgatccg
     2941 aaaggtgacg agtgggaaaa atatgctggc gccacggtga ttaccccgaa ccgctccgaa
     3001 ttgcgcgatg tcgtcggccg ctggtcgtcg gagcaaatgc tcttcgacaa ggccgagaag
     3061 ctgcgcggcg aacttggtct tgaggccttg ctggtcaccc gcagcgagga aggcatgacg
     3121 cttttcgccg acggccagac tttccaccag gcggc
//
LOCUS       DAOSDX010000271         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10882_length_3153_cov_2.782763, whole genome shotgun sequence.
ACCESSION   DAOSDX010000271 DAOSDX010000000
VERSION     DAOSDX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10882_length_3153_cov_2.782763"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="panC"
                     /locus_tag="PLN02_06995"
     CDS             complement(<1..609)
                     /gene="panC"
                     /locus_tag="PLN02_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOI52110.1"
                     /translation="MQIHSSIADLRAALSQRGRIVFIPTMGNLHAGHISLMEQARAHG
                     DTVVASIFVNRLQFGPNEDFDKYPRTFQADCDKLAAAGVDVLFAPTEADLYPEPQEYL
                     IEPPGIQNILDGEFRPGHFRGVATVVTKLFNCVQPQVALFGKKDYQQLMMIRNMTRQL
                     ALPIAIVGGETVRAEDGLALSSRNGYLSEAERAEAPRLYRTLN"
     gene            complement(638..1456)
                     /gene="panB"
                     /locus_tag="PLN02_07000"
     CDS             complement(638..1456)
                     /gene="panB"
                     /locus_tag="PLN02_07000"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOI52111.1"
                     /translation="MSAQTVTRRLTQGDLAKLYQAGEKVVMFTCYDASFARLLDGAGI
                     DCLLIGDSLGNVIQGHDTTLPVTVADIAYHTAAVKRGSDRAFILADMPFGSYQESPEQ
                     AFRNAVALMAAGAQMVKLEGGAEMAATVRFLVSRGIPVCGHLGLTPQSVHVLGGYKVQ
                     GKGEAAAQRLKDDALALQEAGATMIVLEAIPAALAAEVTAALHLITIGIGAGKDCSGQ
                     VMVVHDAFDIPPGRKAKFVRNFMDGASSLHDAACRAAAAVRDGSYPGPEHTYAG"
     gene            complement(1518..2000)
                     /gene="folK"
                     /locus_tag="PLN02_07005"
     CDS             complement(1518..2000)
                     /gene="folK"
                     /locus_tag="PLN02_07005"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOI52112.1"
                     /translation="MAIAYIALGANLGDPASTLRAAFAALDSVPDSRLLARSALYLSA
                     PVGLTGQPDFVNAAARIETTLAPAALLDALFAIEQQFGRLRGERNGPRTLDLDLLLYD
                     QQVITTPRLTLPHPRLHLRAFVLLPLADLDHALSIPGRGTLAAWLPAVATQGIRRLDA
                     "
     gene            complement(2002..>3153)
                     /gene="pcnB"
                     /locus_tag="PLN02_07010"
     CDS             complement(2002..>3153)
                     /gene="pcnB"
                     /locus_tag="PLN02_07010"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOI52113.1"
                     /translation="AVRDLLIGHPPKDYDVATDATPEEVKRAFRRSRIIGRRFQIVHV
                     MMGQEQIEVTTFRGQLSEDTKKDEHGRVLHDNVFGSQAEDAARRDFTANALYYDPTTE
                     TVIDYHHGVGDLKAKTLRMIGEPRARYREDPVRMLRAVRLAAKLGLAIDPAAKKPIRE
                     MAGLLENVPAARLFDEMLKLLTSGHAVKCITQLRDEGLHHGLLPLLDVILEQPMGERF
                     VMAALANTDERIRRGKGSSPGFLFATLLWHEVLAHWDKLMAAGETKIPALYQAMDTVL
                     DVQAEKLAITRRIAGDIKEIWALQPRFEQRTGKRPYALLEHPRFRAGYDFLLLRGEAG
                     EIDSEIGAWWTAFQHADGEGRASMLLPEATGDKKKRRRKRKKPAADPEV"
BASE COUNT          466 a          938 c         1194 g          555 t
ORIGIN      
        1 gttcagcgtg cggtagaggc gtggcgcttc agcgcgctcg gcctcgctca gatagccgtt
       61 gcgcgacgac agcgcgaggc cgtcttcggc acgcaccgtc tcgccgccga cgatggcgat
      121 cggcagcgcc agttggcgcg tcatgttgcg gatcatcatc agctgctggt aatccttctt
      181 gccgaacagc gcgacctgcg gctggacgca gttgaacagc ttggtgacga cggtggcgac
      241 gccgcggaaa tggccggggc ggaattcgcc gtcgaggata ttctggatcc ccggcggctc
      301 gatcaaatat tcctgcggct ccgggtagag gtcggcctcg gtcggcgcga acaggacatc
      361 gacgccagcg gcggcgagct tgtcgcaatc ggcctggaag gtgcgcgggt atttgtcgaa
      421 atcctcgttc gggccgaact gcaggcgatt gacgaagatt gatgcgacca cggtgtcgcc
      481 gtgcgcgcgg gcctgttcca tcaggctgat gtggccggcg tgcaggttgc ccatggtcgg
      541 gatgaagacg atgcggccgc gctgggacag ggcggcgcgc aggtcggcga tgctggagtg
      601 gatttgcatg ggactgggtt gagtttggcc ggcgggatca accggcgtag gtgtgttccg
      661 ggccgggata gctgccgtcg cggacggcgg cggcggcacg gcaggcggcg tcgtgcagcg
      721 aactggcgcc gtccatgaag ttgcggacga acttggcttt gcggccgggc gggatgtcga
      781 aggcgtcatg aacgaccatg acctggccgg agcagtcctt gccggcgccg atgccgatgg
      841 tgatgaggtg cagcgcggcg gtgacttcgg cggccagcgc ggcggggatc gcttccagga
      901 cgatcatcgt cgctccggct tcctgcagtg ccagcgcgtc gtccttcagg cgctgcgcgg
      961 cggcctcgcc cttgccctgg accttgtagc cgccgagcac gtgcaccgac tgcggcgtca
     1021 gaccgaggtg gccgcagacc gggatgccgc ggctgaccag gaagcgtacc gtcgctgcca
     1081 tctcggcgcc gccttcgagc ttgaccatct gggcgccggc ggccatcagc gcgacggcgt
     1141 tgcggaaggc ctgttcgggc gattcctgat agctgccgaa cggcatgtcg gcgaggatga
     1201 aggcgcggtc ggaaccgcgc ttgacggcgg cggtgtggta agcgatgtcg gcgacggtca
     1261 ccggcagcgt cgtgtcgtgg ccctggatga cgttgcccag cgagtcgccg atcagcaggc
     1321 agtcgatccc ggcgccgtcg agcaggcgcg cgaagctggc gtcgtagcag gtgaacatga
     1381 cgaccttttc gccagcctgg taaagcttgg ccagatcgcc ctgggtcagg cggcgggtga
     1441 cggtttgtgc ggacatgggg gattcgctcc tgtgaatcgg acgcctagga tacacagcgg
     1501 cgccgtgggt gcaagtgtca ggcgtcgagc cggcggatgc cctgggtagc gaccgccggc
     1561 agccaggcgg cgagggtacc gcgaccggga atgctcaagg catgatcaag gtcggccagc
     1621 ggcagcagga cgaaggcgcg caggtgcagg cgcgggtgcg gcagggtcag acgcggcgtc
     1681 gtgatgacct gctggtcgta gagcagcagg tcgaggtcga gggtgcgcgg gccgttacgt
     1741 tcgccacgta agcgaccgaa ctgctgttcg atggcgaaca gcgcgtcgag cagggctgct
     1801 ggggctagcg tggtttcgat gcgggcggcg gcattgacga agtccggctg gccggtcagg
     1861 ccgaccggcg cgctcaggta gagcgccgaa cgggcgagca ggcggctgtc cggcacggaa
     1921 tcgagcgcgg caaaggcggc acggagcgtg gacgccgggt cgccgagatt ggcgcccagg
     1981 gcgatgtagg caatggccat ctcagacttc gggatcagcg gccggtttct tgcgcttgcg
     2041 ccggcgcttc ttcttgtcgc cggtggcttc cggcagcagc atgctggcac ggccttcgcc
     2101 gtcggcgtgc tggaaggccg tccaccacgc accgatttcg ctgtcgattt caccggcttc
     2161 gccgcgcagc agcaggaagt cgtaaccggc gcggaagcgc ggatgttcga gcagggcgta
     2221 ggggcgcttg ccggtgcgct gttcgaagcg cggctgcagg gcccagattt ccttgatgtc
     2281 gccggcgatg cggcgggtga tcgcgagttt ttcggcctgt acgtcgagca ccgtgtccat
     2341 cgcctgataa agcgccggaa tcttcgtttc gccggcagcc atgagcttat cccaatgcgc
     2401 cagcacttcg tgccagagca gggtggcgaa caggaaaccg ggcgaactgc ccttgccgcg
     2461 gcggatgcgc tcgtcggtgt tggccagcgc cgccatcacg aagcgttcgc ccatcggctg
     2521 ttcgaggatc acgtcgagca gcggcagcag gccgtggtgc aggccctcgt cgcgcaactg
     2581 ggtgatgcac ttgacggcgt gtccggaggt caagagcttg agcatctcgt cgaacaggcg
     2641 ggcggccggg acgttttcca gcaggccggc catctcgcgg atcggctttt ttgctgccgg
     2701 gtcgatggcg aggccgagct tggccgccag gcgcacggcg cgcagcatgc gcaccgggtc
     2761 ctcgcggtag cgggcgcgcg gctcaccgat catgcgcagc gtcttcgcct tgaggtcgcc
     2821 gacgccgtgg tggtagtcga tcaccgtttc ggtggtcggg tcgtagtaca gcgcgttggc
     2881 ggtgaagtcg cggcgcgccg cgtcttccgc ctggctgccg aagacgttgt cgtgcagcac
     2941 gcggccgtgt tcgtccttct tggtgtcttc gctgagctgg ccgcggaagg tggtgacctc
     3001 gatctgctcc tggcccatca tcacgtgcac gatctggaag cggcggccga tgatgcgcga
     3061 gcggcggaag gcgcgtttga cctcttccgg cgtggcgtcg gtggcgacgt cgtagtcctt
     3121 gggcgggtgg ccgatcagca ggtcgcgaac tgc
//
LOCUS       DAOSDX010000272         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10909_length_3146_cov_2.703656, whole genome shotgun sequence.
ACCESSION   DAOSDX010000272 DAOSDX010000000
VERSION     DAOSDX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10909_length_3146_cov_2.703656"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /gene="ligA"
                     /locus_tag="PLN02_07015"
     CDS             complement(<1..1615)
                     /gene="ligA"
                     /locus_tag="PLN02_07015"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOI52114.1"
                     /translation="MPAEIAARAAWLRAELERHNHAYYVLDAPTIPDADYDALFRELQ
                     ALEAEYPALVTVDSPTRRVGGSPLAQFAPVVHDVPMLSIQTETDTGPSGALNFDARVR
                     RELELPAAAPAIAYAAELKFDGLAVSLRYENGVLVRGATRGDGATGEDVTQNLRTVGQ
                     IPLRLQGEAPPLLEVRGEVYMRRDDLEKYNAAATARGEKTLVNPRNAAAGSVRQLDPA
                     IAAGRPLKFFAYGIGAVAGWDEPKTHSGLLDALAAFGLPVCEHRAVLQGGEALAGFHQ
                     QIAELRERLPFDIDGVVYKVDDRELQRRLGFRSREPRWAVAHKYPPQEASTVLEAIDI
                     QVGRTGALTPVARLQPVFVGGVTVTNATLHNADEIERKGGICVGDTVIVRRAGDVIPQ
                     IVGIVAERRPANARPFVMPDACPVCGAHTVREPGEAATRCSGGFSCSAQRTQAILHFA
                     GRRMMDIEGLGDRYVERLVEFGYVHGVADLYQLSLADLLEMKQRADEKDDAASLGEPI
                     PCGADGAPAANRGSEADALKTGQTPTRWAE"
     gene            complement(1615..2919)
                     /locus_tag="PLN02_07020"
     CDS             complement(1615..2919)
                     /locus_tag="PLN02_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HOI52115.1"
                     /translation="MTELQLGMIGLSATVVVGVYAYNKWQEYRHRKVAEAMLKPGHDD
                     VLLNGGARPARREKQPERSPEKPVERVEPACAAPVADAVRHEMPPQNDPEPVVSVAEL
                     PDEAPVPVFMPLEEETDLPPVFADEPVEEPVPRRPVIDSVPASPPVERTPAEIPQVAL
                     PPELLDPRIELIVAMELVEPVSSRQILVSQRDVLRRLGKPVHWVGFDESRRKWLPLHS
                     DEDIPVRRLRIGLPLVNRLGPVAEGDLALFNTAMQALADELMAVADLPSSRPLDQAAE
                     IDRFCAAVDLEIGLNLVSRGIPFPGTKIRALAEAAGMTLGVDGLFTRYDDAGRPQFSL
                     QNYENTLFSGDSLRTLTTHGITFLLDVPRVEHGEAVFVQMVAQAQRFAEVLQGTLVDD
                     NRNPLGESQLDHIRREFIGKPQATMRSFGLPAGSPQALRLFS"
     gene            complement(2916..>3146)
                     /locus_tag="PLN02_07025"
     CDS             complement(2916..>3146)
                     /locus_tag="PLN02_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52116.1"
                     /translation="PFCLLDEVDAPLDDSNTERFCSMVKKMSGATQFLFISHNKIAME
                     MAEQLVGVTMQESGVSRVVEVDIEEALKMRDA"
BASE COUNT          492 a         1060 c         1065 g          529 t
ORIGIN      
        1 tctccgccca gcgcgtcggc gtctggccgg tcttcagcgc atccgcttcg ctgccgcgat
       61 tcgccgccgg ggcgccgtcg gcgccgcacg ggatgggctc gccgagactt gccgcgtcat
      121 ccttttcgtc ggcacgctgc ttcatttcca acaggtcggc caagctcaac tgatagagat
      181 cggcgacccc atgcacgtag ccgaactcga ccaggcgctc gacgtagcgg tcacccaggc
      241 cttcgatatc catcatgcgc cggccggcga aatgcaggat cgcctgagta cgctgggcgc
      301 tacacgagaa accgccggaa cagcgggtcg ccgcctcgcc cggctcgcgc accgtgtgtg
      361 caccgcatac cgggcaggcg tcgggcatga cgaagggccg ggcgttcgcc ggccgccgtt
      421 cggcgacgat accgacgatt tgcggaatga cgtcgccggc gcggcggacg atcaccgtat
      481 cgccgacgca gatgccgccc ttgcgctcga tctcgtcggc gttgtgcagg gtcgcattgg
      541 tcaccgtgac gccgccgacg aacaccggct gcaggcgtgc caccggcgtc agcgcgccgg
      601 tgcggccgac ctggatgtcg atcgcctcga gcaccgttga tgcctcctgc ggcggatact
      661 tgtgcgccac cgcccagcgc ggctcgcgcg agcggaagcc gaggcggcgc tgcagttcgc
      721 ggtcgtcgac cttgtacacc acgccgtcga tgtcgaatgg caggcgctcg cgcagttcgg
      781 caatttgctg gtggaagccg gccagcgcct cccccccttg cagcacggcg cggtgctcgc
      841 agaccggcag tccgaaggcg gcgagcgcgt cgagcaaacc actgtgcgtc ttcggctcgt
      901 cccagccggc gaccgcgccg atgccgtaag cgaagaattt cagcggccgg ccggcggcga
      961 tcgccggatc gagctggcgg acgctgccgg cggcggcgtt gcgcggattg accagggtct
     1021 tttcaccgcg cgccgtggcg gcggcgttgt atttctcgag atcgtcgcgc cgcatgtaca
     1081 cctcgccgcg cacttccagc agcggcggcg cctcgccctg caggcgcagc gggatctggc
     1141 cgacggtgcg caggttctgg gtgacgtcct cgccggtggc accgtcgccg cgtgtcgcac
     1201 cgcgcaccag cacgccgttc tcgtagcgca ggctgaccgc cagaccgtcg aacttcagtt
     1261 cggcggcgta ggcaatggcc ggcgcagcgg ccggcagttc cagctcgcgg cggacgcggg
     1321 cgtcgaaatt gagcgcgccg ctcgggccgg tgtcggtctc ggtctggatc gacagcatgg
     1381 gtacgtcatg caccaccggc gcgaactgcg ccagcggcga gcctccaacg cgccgggtcg
     1441 gcgaatccac cgtcaccagc gccggatatt cggcttccag cgcctgcagc tcacggaaca
     1501 gcgcgtcgta atcggcgtcc gggatggtcg gcgcgtcgag gacgtagtag gcgtgattat
     1561 gacgttccag ttcggcgcgc agccaggccg cgcgtgccgc gatttccgcc ggcatcacga
     1621 gaacaggcgc aaggcttgcg gcgaaccggc gggcaggccg aaggaacgca tcgtcgcctg
     1681 cggtttgccg atgaactcgc ggcggatgtg atcgagctgg ctttcgccga gcggattacg
     1741 gttgtcgtcg accagcgtgc cctgcagcac ttcggcaaag cgttgcgcct gggcgaccat
     1801 ctgcacgaac accgcttcgc cgtgctcgac gcgcggcacg tcgagcagga aggtgatgcc
     1861 atgcgtcgtc agggtgcgca ggctgtcgcc ggagaacagg gtgttctcgt agttctgcag
     1921 gctgaactgc ggccgaccgg cgtcgtcgta gcgggtgaac aggccatcga cgccaagcgt
     1981 catgccggcg gcttcggcca gcgcgcggat cttggtgccc gggaagggaa tgccacggct
     2041 gaccaggttg aggccaatct caaggtcgac ggcggcgcag aaacgatcga tttccgccgc
     2101 ctggtcgagc gggcgcgacg acggcaggtc ggccaccgcc atcagttcgt cggccagcgc
     2161 ctgcatcgct gtgttgaaca gcgcgaggtc gccctccgcc accggcccga ggcgattgac
     2221 caggggcagc ccgatgcgca ggcgacgcac cgggatgtcc tcgtcgctgt gcaagggcag
     2281 ccatttgcgg cggctttcgt cgaagccgac ccaatgcacc ggcttgccga gacggcgcag
     2341 cacatcgcgc tgcgaaacaa ggatctgccg cgacgagacc ggctcgacca actccatggc
     2401 aacgatcaat tcgatgcgtg gatcgagcag ttccggcggc aaggcgacct ggggaatttc
     2461 cgccggcgtc cgttcgacgg gcggcgacgc gggaacgctg tcgatgaccg ggcggcgagg
     2521 aacgggctct tcgacgggtt cgtcggcaaa aaccggcggc aaatccgtct cttcttccag
     2581 cggcatgaac accgggacgg gcgcctcgtc aggcagctcg gcgacggaaa cgaccggctc
     2641 cggatcgttc tgcggcggca tctcatgccg gaccgcgtcg gcgaccggcg ccgcacaggc
     2701 cggttccact cgctccactg gtttctccgg cgaccgctcc ggctgttttt cccgccgtgc
     2761 cggacgggca ccgccgttga gcaggacgtc gtcgtgcccg ggtttgagca tcgcctccgc
     2821 gaccttgcgg tgccggtatt cctgccattt gttgtaagcg tacacgccga ccacgacggt
     2881 cgcgctcagg ccgatcattc ccagttggag ttcggtcatg catccctcat tttcaaggct
     2941 tcttcgatat ccacttccac cacgcgcgag acgccgcttt cctgcatggt gacgccgacc
     3001 agctgctcgg ccatttccat cgcgatttta ttatgggaaa tgaacaggaa ctgcgttgcc
     3061 cctgacattt tcttgaccat gctgcagaaa cgctcggtgt tggagtcgtc gagcggcgca
     3121 tcgacttcgt cgagcaggca gaacgg
//
LOCUS       DAOSDX010000273         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_10968_length_3135_cov_3.583442, whole genome shotgun sequence.
ACCESSION   DAOSDX010000273 DAOSDX010000000
VERSION     DAOSDX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10968_length_3135_cov_3.583442"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="PLN02_07030"
     CDS             <1..2127
                     /locus_tag="PLN02_07030"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOI52117.1"
                     /translation="PTLVVVTDRNDLDGQLFQTFAGARALLKEMPQQASDRDELRALL
                     DGRPSGGIIFTTVQKFAPGPDEAVFPLLSARTNIVVIADEAHRSQYGFRAVLDKKTGQ
                     YKYGFAKHLRDALKNATFVGFTGTPVESSDKDTRTVFGDYVSIYDIQDAVDDGATVPI
                     YYESRLARLDLNQAEMEQLNSDVEEVFEDEEDVALREAAKTRWAALEKLVGAQPRIDQ
                     VAADIVQHFEARNTAVAGKGMIVGMSRDICARLYQAIVALRPEWHSDDPAQGAIKVVM
                     TGSAADSELLRPHIYNAATKKLLEKRFKDANDSLKLVIVRDMWLTGFDVPCLHTMYVD
                     KPMRDHNLMQAIARVNRVFRDKEGGLVVDYIGIAAELRNALKAYTESRGKGQPTLDAA
                     EALAKLKELMDVARGLLHGFEFGAFATQAVALLLPAANFVLGLEDGKKRWADVVLAIT
                     KAFSLCGTLDEALALREEIAFFQAIKAVIAKATTSEKRLSEDQKNAVLKQILDNAVVA
                     EGVEDIFKLAGLERPDIGILSDAFLEEVRSLPQRNFAVELLQKLLNDEVKSRSRTNVV
                     LERKFSERLQAALNRYRGRAIESAQVIEELIAMAKEFRAAAQRGDALGLNESELAFYD
                     ALADNESAVREMGDEMLKKIAHELTEKLRNSTTVDWQKRDSVRARLRNLVRITLRRYK
                     YPPEMQEEAIRLVLDQAERLSDAWSR"
     gene            complement(2270..>3135)
                     /locus_tag="PLN02_07035"
     CDS             complement(2270..>3135)
                     /locus_tag="PLN02_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI52118.1"
                     /translation="FAAVARLRSFRKAAAELGVSPSALSHALRGLEARLGVRLLNRTT
                     RSVAPTEAGERLLDRLSPALQEIHGALDEVNAFRDSPLGTLRINAPRAACELVLAPLL
                     GRFLRANPGMRIELVADDAFVDIVATGFDAGVRFGESLQQDMVAVPLGPPQRFVVVAS
                     PDYLAAHGTPECPRELQQHRCIRIRFPNGSFYRWEFARGSEQFEIEVDGPVSVGQMPL
                     MIAAAEQGLGLAYVYAQYAAAPIAAGRLQTVLDDWCPEIPGFYLYYPSRKLMPAGLKA
                     FVEMLQERSEF"
BASE COUNT          603 a          944 c         1077 g          511 t
ORIGIN      
        1 ccgacgctgg tcgtggtgac cgaccgcaac gatctcgacg gccagttgtt ccagaccttt
       61 gccggggcgc gggcgttgct gaaggagatg ccgcaacagg cgtccgaccg cgacgagttg
      121 cgcgccttgc tcgacggccg accgtccggc ggcatcattt tcacgaccgt gcagaaattc
      181 gcaccggggc cggatgaggc ggtcttcccg ctgctttcgg cgcgcaccaa catcgtcgtc
      241 atcgccgacg aggcgcaccg ctcgcagtac ggttttcgcg ccgtgctgga caagaagacc
      301 ggccagtaca agtacggttt cgccaagcat ctgcgcgatg ccttgaagaa cgccaccttt
      361 gttggtttca ccgggacccc ggtggagagc agcgacaagg atacgcgcac ggtgttcggc
      421 gactacgtca gcatctacga catccaggac gcggtggatg acggtgccac ggtgccgatc
      481 tactacgaga gccgcctggc ccggttggac ctcaaccagg ccgagatgga acagctcaac
      541 agcgatgtcg aagaggtgtt cgaggacgag gaagacgtcg cgctgcgcga ggcggccaag
      601 acgcgctggg cggcgctgga aaagctggtc ggcgcgcagc cgcgcatcga ccaggtggcg
      661 gcggacatcg tccagcactt tgaggcgcgc aatacggcgg tggccggcaa gggcatgatc
      721 gtcggcatga gccgcgacat ctgcgcccgg ctctaccagg caattgttgc gctgcgccct
      781 gaatggcaca gcgacgaccc ggcgcagggc gcgatcaagg tggtgatgac cggctcggcg
      841 gcggatagcg aactgttgcg cccgcatatc tacaacgcgg ccaccaagaa gctgctggaa
      901 aagcgcttca aggatgccaa cgacagcctc aagctggtga tcgtgcgcga catgtggctg
      961 accggcttcg atgtgccctg tctgcacacg atgtacgtgg acaagccgat gcgcgaccac
     1021 aacctgatgc aggccatcgc gcgggtgaac cgggttttcc gcgacaagga aggcgggctg
     1081 gtggtcgatt acatcgggat tgccgccgag ttgcgcaatg ccttgaaggc ctatacggaa
     1141 agccggggca aggggcagcc aacgctggat gcagccgagg cgctggccaa gctgaaagag
     1201 ttgatggatg tggcgcgcgg gctgctgcat ggcttcgaat tcggtgcctt tgccacgcag
     1261 gcggtggcct tgctgctgcc ggcggccaat ttcgtgctcg ggctggagga cggcaagaag
     1321 cgctgggccg acgtggtgct ggccatcacc aaggcgtttt cgctgtgcgg cacgctcgac
     1381 gaggcgctgg ccttgcgcga ggaaattgct ttcttccagg cgatcaaggc ggtgatcgcc
     1441 aaggcaacga cgtccgagaa gcgcttgtcg gaagaccaga aaaatgccgt cctcaagcag
     1501 attctcgaca atgccgtggt ggccgagggc gtcgaggaca tcttcaagct ggccgggctg
     1561 gagcggccgg acattggcat tctttccgac gcttttcttg aagaagtgcg cagcctgccg
     1621 cagcgtaact ttgcggtgga gttgctgcag aagctgctca acgacgaggt gaaatcccgt
     1681 tcgcggacca atgtggtgct cgaacgcaaa ttcagcgagc gcctgcaggc ggcgctcaac
     1741 cgctaccgtg gccgggccat cgaaagcgcc caggtgatcg aggaactgat cgccatggcc
     1801 aaggagttcc gcgccgccgc gcagcgcggc gatgcgctgg ggctcaacga atccgaactg
     1861 gctttctacg acgcgctggc cgacaacgaa agcgcggtgc gcgaaatggg cgacgaaatg
     1921 ctcaagaaga tcgcccatga gctgaccgaa aagctgcgca acagcacgac cgtcgactgg
     1981 cagaagcggg acagcgtgcg cgcccgcttg cgcaacctgg tgcgcatcac cctgcgccgc
     2041 tacaagtacc cgccggagat gcaggaagag gcgatccggc tggtgctgga tcaggcggag
     2101 cggctttcgg atgcgtggtc gcgttgaaga ctatgcaaag aaattgaaac ctcaggtaga
     2161 gaactgacag gttttctgtt gtacaggtgg agagggtgct gcacattctt gccggtattg
     2221 ccgccagcct taccgggcag acgtcgatcg gatgagagag ccgtagcgat caaaactccg
     2281 accgctcctg cagcatctcg acaaaagcct tcagcccggc cggcatcagc ttgcggctcg
     2341 ggtaatacag gtaaaagccg ggaatttccg ggcaccagtc gtccagcacc gtttgcaagc
     2401 gccctgccgc gattggcgcc gccgcgtact gggcatagac gtaggccagc ccgagacctt
     2461 gttccgctgc cgcgatcatc agcggcatct ggccgacgct gaccggcccg tcgacttcga
     2521 tttcgaactg ttcgctgccg cgcgcgaatt cccagcgata gaaactgccg ttggggaaac
     2581 ggatgcggat gcagcggtgc tgctgcaatt cgcgcgggca ttcgggtgtg ccgtgcgccg
     2641 ccagatagtc gggcgaggcg accacgacaa agcgctgggg cgggcctagc ggtacggcga
     2701 ccatgtcctg ctgcaggctt tcgccgaagc ggacgccggc atcgaagccg gtggcgacga
     2761 tatcgacgaa ggcgtcgtcg gccaccagtt cgatgcgcat gcccgggttg gcccgcagaa
     2821 agcgcccgag cagcggcgcc agcaccagtt cgcacgccgc tcgcggcgcg ttgatgcgca
     2881 gcgtgccgag cggcgagtcg cggaaggcat tgacttcgtc gagcgcgccg tggatctcct
     2941 gcagggcggg cgagaggcgg tcgagcaggc gctcgccggc ctcggtcggc gccacgctgc
     3001 gtgtcgtccg gttgagcagg cggacgccga gccgggcctc caggccgcgc aaggcgtgac
     3061 tcagcgccga cggtgaaacg ccaagttcgg cggccgcctt gcgaaagctg cgcaggcggg
     3121 cgacggcggc aaacg
//
LOCUS       DAOSDX010000274         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11010_length_3127_cov_3.800130, whole genome shotgun sequence.
ACCESSION   DAOSDX010000274 DAOSDX010000000
VERSION     DAOSDX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11010_length_3127_cov_3.800130"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLN02_07040"
     CDS             <1..399
                     /locus_tag="PLN02_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOI52119.1"
                     /translation="VFRHCGAIPIASAKEDPALMEKAFAEVAAALADGQLVGIFPEGN
                     ITADGELQPFRPGVARILAANPVPVVPMALSGLWGSFFSRIDGVAMKTPFRRGMFSAI
                     GLKVGAPLDAAAATPERLFESVLALRGERR"
     gene            439..1197
                     /locus_tag="PLN02_07045"
     CDS             439..1197
                     /locus_tag="PLN02_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52120.1"
                     /translation="MSVSPSIPADSLAGLADADLQRQAARLAQDVFAGVFRQAVSADE
                     ADDTARLADLGNRCSNWCRAAGSDDGRALRLALLISGLDQWGLAYSQAFQLTAIPALT
                     ALVGALRSALDAQADARFQRYFAQLDGDEAAAIDFKIELRRAIHLALWHAMAACETGE
                     QAANIVRPLGGLMLALDQRMPELGWRLLADALASIQIRLLQDNSASTVAQEATQTLFA
                     SLRQALPGERFQAILAHSGQAVIAWQRAQRPVVH"
     gene            1208..2080
                     /gene="ubiA"
                     /locus_tag="PLN02_07050"
     CDS             1208..2080
                     /gene="ubiA"
                     /locus_tag="PLN02_07050"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOI52121.1"
                     /translation="MNWQAWRERLDLYEKLMRLDKPIGTLLLLWPTFWALWLSADGRP
                     DWIVVWVFALGTLLMRSAGCVINDYADRDFDKHVERTRERPLTAGRVSTREALALFGA
                     LCLASLALVVALDRPLVVWLSIPAVFLAASYPFTKRFFAIPQAYLGIAFGFGIPMGYA
                     AQLATVPAEAWWMLLANIFWAVAYDTEYAMVDRNDDLKIGIKTSAITFGRFDVAAVMA
                     CYAVTLGILAAIGAQRALAWPYYLGLALAAGMMLRHFFWIRGRERMPCFRAFLDNNRV
                     GAAIFAGLVASYWV"
     gene            2089..2937
                     /locus_tag="PLN02_07055"
     CDS             2089..2937
                     /locus_tag="PLN02_07055"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOI52122.1"
                     /translation="MAVSEGNFARVGLGDIVVGQPLRWNLYNEAREQIRRQGEIFDSL
                     EAIDALVSPGVYRLLSSAEKQSRDVAFDTSRIRVGDAIQLEVSPEQPRYVVFLIGYQK
                     NKGIIVTPPESAGGLMMLRDGQSFVGRFFSGQRAFAFSTTLVRQTSVPFPHMHLAYPR
                     DLRVQEVRKSPRIDVQMIAAIEFVDREAPQASGKICNLSATGAALRTKHRLAPIGEKV
                     RLKFKLAIQGLETYLSVLCEVRSLADNRDDPAMPVLWGLSFVETDSHAHFALSAYVYG
                     MLLGEV"
     gene            2959..>3127
                     /locus_tag="PLN02_07060"
     CDS             2959..>3127
                     /locus_tag="PLN02_07060"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOI52123.1"
                     /translation="MKYHDLRDFLSQLEKTGELKRIAVPVDTHLEMTEIGDRVLRAGG
                     PALLFEKATTGG"
BASE COUNT          459 a         1044 c         1050 g          574 t
ORIGIN      
        1 gtcttccgtc attgcggggc aattccgatt gcttcggcca aggaagaccc ggcgctgatg
       61 gaaaaggctt tcgccgaggt cgcggcggcg ctcgccgacg gccagctggt cggcatcttc
      121 ccggaaggca acatcaccgc cgacggcgaa ttgcagcctt tccggccggg cgttgcccgc
      181 atcctcgccg ccaacccggt gccggtggtg ccgatggcgc tgtccgggct gtggggcagc
      241 ttcttctccc gtatcgacgg ggtggcgatg aagacgccgt tccggcgcgg catgttttcg
      301 gccatcgggc tcaaggtcgg ggcgccgctg gatgccgcgg cggcgacgcc ggagcgactg
      361 ttcgaaagcg tgctggcgct ccggggggag cgccgctgag cggttagaat aggcgctttt
      421 catgtcgtgg atttgaagat gagtgtttct ccgtcgattc ccgccgactc cctggcggga
      481 ttggccgatg ccgatctgca gcgtcaggcg gcgcgcctgg cgcaggatgt gtttgccggc
      541 gttttccggc aggcggtgag cgccgacgag gccgacgaca cggcgcgtct cgccgatctc
      601 ggcaaccgct gcagcaactg gtgccgggcc gccggcagcg acgacgggcg cgccctgcgc
      661 ctggccctgc tgatcagcgg cctcgaccag tgggggctgg cctacagcca ggccttccag
      721 ctgacggcga tcccggcgct gacggcactg gtcggggcgc tgcgcagcgc gctcgacgcc
      781 caggccgacg cccgtttcca gcgctacttc gcgcaactcg acggcgacga ggcggcggcg
      841 atcgatttca agatcgagtt gcggcgcgcc atccatcttg ccctgtggca cgccatggcg
      901 gcctgcgaga ccggcgaaca ggcggcaaac atcgtccgcc cgctgggcgg cctgatgctg
      961 gcgctcgacc agcgcatgcc ggaactcggc tggcgcctgc tggccgatgc gctggccagc
     1021 atccagattc gcctgttgca ggacaattcc gcatccacgg tggcgcagga ggcgacgcag
     1081 accttgttcg catccttgcg ccaggcgctg ccgggcgagc gtttccaggc catcctggcg
     1141 cactccgggc aggcggtgat tgcctggcag cgggcgcagc ggccggtcgt gcattgaggg
     1201 ggcggcgatg aactggcagg cctggcgcga acggctcgac ctctacgaaa aactgatgcg
     1261 gctggacaag ccgatcggca ccctgctgct gctctggccg accttctggg cgttgtggct
     1321 gtccgccgac ggccggccgg actggatcgt cgtctgggtc ttcgccctcg gcaccttgct
     1381 gatgcgctcc gccggctgcg tcatcaacga ttacgccgac cgcgatttcg acaagcatgt
     1441 cgagcgcacc cgcgagcggc cgctgaccgc cggccgggtc agcacgcgcg aagcgctggc
     1501 gctgttcggt gcgctctgcc tggccagttt ggcgctggtc gtcgcactcg accggccact
     1561 ggtcgtctgg ctgtcgattc ccgccgtttt cctggccgcc agctatccct tcaccaagcg
     1621 cttcttcgcc atcccgcagg cctatctcgg catcgccttc ggtttcggca tcccgatggg
     1681 ctacgccgcg caactcgcga cggtgccggc ggaagcctgg tggatgctgc tcgccaacat
     1741 cttctgggcg gtcgcttacg acaccgaata cgcgatggtc gaccgcaacg acgacttgaa
     1801 aatcggcatc aagacctcgg cgatcacctt cggtcgtttc gacgttgccg ccgtcatggc
     1861 ctgctacgcg gtgaccctgg gcatccttgc cgcgatcggc gcgcagcgcg ctctggcctg
     1921 gccgtattac ttggggctgg cgctggccgc aggtatgatg ctcaggcatt tcttctggat
     1981 ccgcggtcgg gagcgcatgc cctgtttccg ggcttttctc gacaacaacc gggtgggggc
     2041 cgcgatcttc gcggggctgg tcgccagtta ctgggtatga ggtaaggcat ggcagtgagt
     2101 gaggggaatt ttgcccgggt cgggctgggc gacatcgtcg tcggccagcc tttgcgctgg
     2161 aatctctaca acgaggcccg cgagcagatt cgccgtcagg gcgagatttt cgattcgctg
     2221 gaggcgatcg acgccctggt ttcccccggc gtctaccgct tgctgtcgag cgccgagaag
     2281 cagtcgcgcg atgtcgcctt cgacacctcg cgcatccgcg tcggcgacgc gatccagctc
     2341 gaagtctcgc cggagcagcc gcgctacgtc gtcttcctga tcggctacca gaagaacaag
     2401 gggatcatcg tcaccccgcc ggaaagcgcc ggcggcctga tgatgctgcg cgacggccag
     2461 tccttcgtcg gccgcttctt ttccgggcag cgcgccttcg ccttctcgac gacgctggtc
     2521 aggcagacct cggtgccgtt cccgcacatg cacctggctt acccgcgcga tctgcgcgtc
     2581 caggaagtgc gcaagagccc gcgcatcgac gtgcagatga tcgccgccat cgagttcgtc
     2641 gaccgcgagg cgccgcaggc ctcgggcaag atctgcaacc tcagcgccac cggtgcggcg
     2701 ctgcgcacca agcaccgcct ggcgccgatc ggcgagaagg tccgcctcaa gttcaagctc
     2761 gccatccagg ggctggaaac ctacctttcg gtgctctgcg aggtccgctc gctggccgac
     2821 aaccgcgacg atccggcgat gccggtgctg tggggactga gtttcgtcga aaccgacagc
     2881 cacgcccatt tcgcgctgtc ggcctatgtt tacggcatgc tgctcggcga agtctgagcg
     2941 ccgagtgacg gagttttctt gaaataccac gacttgcgcg acttcctgtc gcaactggaa
     3001 aagaccggcg aactgaagcg catcgccgtg ccggtcgaca cccatctgga aatgaccgag
     3061 atcggcgacc gcgtgttgcg cgccggcggt ccggcgctgc ttttcgaaaa agcgacgacc
     3121 ggtggcc
//
LOCUS       DAOSDX010000275         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11011_length_3127_cov_3.500326, whole genome shotgun sequence.
ACCESSION   DAOSDX010000275 DAOSDX010000000
VERSION     DAOSDX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11011_length_3127_cov_3.500326"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLN02_07065"
     CDS             complement(<1..236)
                     /locus_tag="PLN02_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOI52124.1"
                     /translation="MFQHGRRPLILARYLALAWCGLIVYGSLHPFAGWRDNGVSPWIF
                     LEGDWPRYWTVFDLAANVAVYLPLGFFVALALYRL"
     gene            complement(268..528)
                     /locus_tag="PLN02_07070"
     CDS             complement(268..528)
                     /locus_tag="PLN02_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52125.1"
                     /translation="MVESAYRITLSGAFVPLFAGLFWKRANNLGAGLAIVLGIGTWLF
                     LEIFVPEGDIEPQLYGLIASTIGMLLGAWLGSRPGLEKTTQG"
     gene            complement(550..1692)
                     /locus_tag="PLN02_07075"
     CDS             complement(550..1692)
                     /locus_tag="PLN02_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOI52126.1"
                     /translation="MLIWFVVIYLAVSIGIGLYAATRVHNARDYIVAGRNLPFPIVLA
                     MVFATWFGAETVLGISATFIDEGFRGLISDPLGASICLMLFGLIFAKRLYRLNLLTLG
                     DFFRVRFNRKVEVALSTAIIISYLGWVGAQMAALGLVFNVLSDGGISLTQGTLIGAGI
                     VLLYTLFGGMWSVAMTTFVQMIVIVLGLLYVTWLAGDMAGGFSAVVGKAAADGKLNFL
                     PSADLLDIVSWIAALITMALGSIPQQDVFQRVNASKNEWVAVWGTTLGGASYFLFAAV
                     PLFLAYSATLIDPALVTRLMAEDSQLILPQLIIQYMPIGAQIIFFGALLSVIMSTASG
                     TLLAPAVTFSENILRGHYPQMTDRQLLLATRAAVIVFTGMVATYAI"
     gene            complement(1735..2415)
                     /locus_tag="PLN02_07080"
     CDS             complement(1735..2415)
                     /locus_tag="PLN02_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOI52127.1"
                     /translation="MQAYCPPTEDPVVLHVDDQLLVVDKPSGLLSVPGRGADKQDCVI
                     SRLQRNWPEAMIAHRLDMSTSGLLVVARGEAVQREMYRLFRERAVDKRYVAAVAGLIA
                     DDTGEIDLPLICDWPNRPRQMVSHELGKPSLTRFRVLARNPALDATVVELEPVTGRSH
                     QLRVHLAALGHPILGDDLYAGAAANRAARLLLHAAEIAFPHPADGRPANFRCPAPFIA
                     LEKAAITL"
     gene            complement(2418..2627)
                     /locus_tag="PLN02_07085"
     CDS             complement(2418..2627)
                     /locus_tag="PLN02_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52128.1"
                     /translation="MKYRLLRYLIPTLLGLSILAAQARPASWHRWFSKIDGTTVCLQT
                     SPGNGWTYGGGPFRDARCSIPDRRS"
     gene            2831..>3127
                     /locus_tag="PLN02_07090"
     CDS             2831..>3127
                     /locus_tag="PLN02_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOI52129.1"
                     /translation="MDDNKAKALAAALQQIEKQFGKGSIMKMGDAEIDEGIQVVSTGS
                     LGLDLALGVGGLPRGRVVEIYGPESSGKTTLCLQVVAEMQKLGGVAAFIDAEHAL"
BASE COUNT          642 a          873 c         1099 g          513 t
ORIGIN      
        1 agacggtaca gcgccagggc gacgaagaaa ccgagcggca ggtataccgc gacgttggcg
       61 gcgagatcga agaccgtcca gtaacgcggc cagtcgcctt cgaggaagat ccacggcgaa
      121 acgccgttgt cccgccagcc ggcaaaggga tgcaggctgc cataaacaat cagcccgcac
      181 caggcgagtg ccaggtagcg ggcgaggatc agcggtcgtc ggccatgttg gaacatggct
      241 gaattctagg cgcagtcggg gattacgtca gccttgcgtg gttttctcga ggcctggacg
      301 ggaaccgagc caggcgccga gcaacatgcc gatggtactg gcgatcagtc cgtacagttg
      361 cggctcgatg tcgccttccg gaacgaagat ttccaggaac agccaggtac cgatgccgag
      421 aacgatcgcc aggccggcgc cgaggttgtt ggcccgcttc cagaagaggc cggcgaacag
      481 cgggacgaag gcgccggaca gggtgatccg gtaagcgctt tcgaccatgt ggtggatggt
      541 ggcgttggac taaatggcgt aggtggcgac catgccggtg aacacgatga cggcggcacg
      601 cgtggcgagc agcagttggc ggtcggtcat ctgcgggtag tggccgcgca ggatgttttc
      661 ggagaaggtg acggccggtg ccagcagcgt gccggaagca gtactcatga tcaccgagag
      721 caaggcgccg aagaagatga tctgggcgcc gatcggcatg tactggatga tcagttgcgg
      781 caggatcagt tgagaatcct cggccatcag gcgcgtcacc agtgccggat cgatcaacgt
      841 tgccgaatag gcgaggaaga gcgggacggc ggcgaacaag aaataggacg caccgcccag
      901 cgtggtgccc cagaccgcga cccattcgtt cttcgacgcg ttgacccgct ggaagacgtc
      961 ctgctgcggg atcgagccga gcgccatggt gatcagcgcg gcgatccagg agacgatgtc
     1021 gagcaggtcg gcgctgggca ggaaattcaa cttgccgtcg gccgccgcct tgccgacgac
     1081 ggccgagaaa ccgccggcca tgtcgccggc cagccaggtg acgtaaagca ggccgaggac
     1141 gatgacgatc atctgcacga aggtggtcat cgccaccgac cacatgccgc cgaacagcgt
     1201 gtacagcagg acgatgccgg cgccgatcag ggtaccctgg gtgagcgaga tgccaccgtc
     1261 gctgaggacg ttgaagacca ggccgagggc agccatctgg gcaccgaccc agccgaggta
     1321 ggaaatgatg atggccgtcg atagcgcgac ttcgaccttg cggttgaagc ggacccggaa
     1381 gaagtcgccg agggtcagca ggttaaggcg gtaaagccgt ttggcgaaga tcaggccgaa
     1441 cagcatcagg cagatcgagg cgccgagcgg gtcggaaatc aggccgcgga atccttcgtc
     1501 gatgaaggtc gcggaaatgc cgagtacggt ttcggcacca aaccaggtgg cgaagaccat
     1561 cgccagcacg atgggaaacg gcaggttgcg gccggcgacg atgtagtcgc gggcattgtg
     1621 tacccgggtt gcggcgtaga ggccgatgcc gatggaaacg gcgagataga tgacgacgaa
     1681 ccagatcagc atgatggcgg gtcgactacg gcaaggggtt gaaaatgcgc ggaattataa
     1741 ggttattgcg gctttttcca gcgcgataaa tggcgccggg cagcgaaaat ttgccggtcg
     1801 cccatccgcc gggtgcggga aggcaatttc tgccgcgtgc agcagcagtc gtgccgcccg
     1861 gttggcggct gcgccggcgt acagatcgtc gccgaggatg gggtggccga gggcggccag
     1921 atggacgcgc aactggtgcg agcggccggt gaccggttcc agttcgacga ccgtcgcgtc
     1981 cagcgccgga ttgcgggcga ggacccggaa gcgcgtcagc gacggcttgc cgagttcgtg
     2041 actgaccatt tgccggggtc ggtttggcca gtcgcagatc agcggcaggt cgatttcgcc
     2101 cgtgtcatcg gcgatcagcc cggcgacggc ggcgacgtag cgcttgtcca ccgcccgctc
     2161 gcggaacagg cggtacatct cgcgctgcac cgcttcaccg cgggcgacga cgagcaggcc
     2221 ggaagtggac atgtcgaggc ggtgggcgat catcgcctcc ggccagttgc gttgcaggcg
     2281 gctgatcacg cagtcctgct tgtcggcgcc gcgaccgggc acggaaagca gcccgctcgg
     2341 cttgtcgacg accagcaact ggtcgtcgac atggaggacg acgggatctt cggtgggtgg
     2401 gcagtaagcc tgcatgttca ggaacggcgg tccgggatgc tgcagcgggc gtcgcggaac
     2461 ggcccaccgc cataagtcca gccgttacca ggcgaggtct gcaggcagac tgtcgtcccg
     2521 tcgatcttgc tgaaccagcg gtgccagctt gccgggcgag cctgggcggc gaggatggag
     2581 agaccgagta aggtcgggat caggtagcgg agaagacgat atttcatgat cgatatactg
     2641 tatagttgta cagtaaatac tgtccgtcag gcaggggcgc aaagcataca cccctgagtg
     2701 tggcattcgg cagcgaatcg atttgccatt ggcatgttcg ctgccgatgg cgcatcatga
     2761 tgcagccagc aatttttgtt ccatccctgg cccctgtgcc ataatccgct caacgtttcg
     2821 aggtaagaac atggacgaca acaaggcaaa ggcgctcgcc gcagcgctgc aacaaatcga
     2881 aaagcagttc ggcaagggct ccatcatgaa gatgggcgat gccgagatcg acgaaggcat
     2941 tcaggtcgtg tcgaccggtt cgctcgggct cgatctggca ttgggtgtcg gcggcttgcc
     3001 gcgcggtcgt gtcgtcgaga tctacggtcc ggagtcgtcg ggcaaaacca cgctctgtct
     3061 gcaggtcgtc gccgagatgc agaagctggg cggcgttgct gccttcatcg atgccgaaca
     3121 tgccctc
//
LOCUS       DAOSDX010000276         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11023_length_3124_cov_2.952753, whole genome shotgun sequence.
ACCESSION   DAOSDX010000276 DAOSDX010000000
VERSION     DAOSDX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11023_length_3124_cov_2.952753"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /gene="mnmC"
                     /locus_tag="PLN02_07095"
     CDS             <1..1156
                     /gene="mnmC"
                     /locus_tag="PLN02_07095"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HOI52130.1"
                     /translation="GCATASALAGAGWQVEVIDSAATPATGASNNIAGVLRPLPSADD
                     NRLSRLTRAGYLATRTLLQTLPDARWSPCGVLHLGRDAAHEATQRRVVERLQLPPELL
                     QFAAADAVAERLGRPVETGGWWFPQGGWVNPASVCRAALAAFPQRIATRYGVAGDRLE
                     RTDDGWRLLAADGSEIARAPVVILAAGAGATHFAQFAGQPQKGARGQISHLPAASITP
                     LPHVVCRHGYLMPEIDGICLTGATLQPGDADLTLRPADHAENLARLQRSLPGFATAID
                     PATLSGRCGVRPMSPDRLPIVGPLPDLAALKGQPRLPALPRHPGLWCVQGFGARGIVW
                     SALMADLLLSRLEGEPLPLENDLVDAVDPGRFLLKPAGRMQEDDGFDDRT"
     gene            1270..2541
                     /locus_tag="PLN02_07100"
     CDS             1270..2541
                     /locus_tag="PLN02_07100"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOI52131.1"
                     /translation="MKIETLAVHGGYSPDPTTKAVAVPIYQTTSYAFDSTQHGADLFD
                     LKVAGNIYTRIMNPTQDVLEKRVAAMEGGVAALAMASGMAAITASIQTIAEAGDNIVT
                     SSTLYGGTYNLFAHTLPQYGIEVRFADYRDPDAFEKLIDARTKAVFCESVGNPLGNVV
                     DFEKLAEVAHKHGVPVIVDNTVPSPYLCRPFEHGADIVVHALTKYLGGHGNSIGGIIV
                     DSGKFPWAEHKQKFKRLNEPDVSYHGVVYTEALGAAAYIGRARVVPLRNMGAAISPFN
                     AFLILQGIETLALRMDRICDNSLKIAEFLKTHPKVSWVNYAGLPDHKDHALVQKYMGG
                     RASGILNFGVKGGREGGGKFQDALQLVTRLVNIGDCKTLACHPASTTHRQLSPEELAK
                     AGVSEDMIRLSVGIEHVDDLIADLDQALNAA"
     gene            2818..>3124
                     /locus_tag="PLN02_07105"
     CDS             2818..>3124
                     /locus_tag="PLN02_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HOI52132.1"
                     /translation="MSDPTALGVFGLSMVTFVAASAKMGWTTGVAYLIPWALFLGSLA
                     QIWASMVDFKKNNYFGAIVLGAYGLFWIAVAMHWAISLGWLGEVGANADPKQLAFACI
                     "
BASE COUNT          550 a         1143 c          933 g          498 t
ORIGIN      
        1 cggctgcgcc acggccagcg cactggccgg cgccggctgg caggtcgagg tgatcgacag
       61 cgcggcgacg ccggccaccg gtgcctccaa caacatcgcc ggggtgctgc ggccgctacc
      121 cagcgccgac gacaaccgcc tgtcgcgcct gacccgcgcc ggctacctgg ccacccgcac
      181 cctcctgcag acgctgccgg acgcccgctg gtcgccctgc ggcgtgctgc acctcggccg
      241 cgacgcggcg catgaagcga cccagcggcg cgtcgtcgag cgcctgcaac tgccgcccga
      301 actgctccag ttcgccgccg ccgacgccgt tgccgaacgc ctcggccgac cggtggaaac
      361 cggcggctgg tggttcccgc agggcggctg ggtcaatccg gcctcggtct gccgcgccgc
      421 gctggcggct tttccccaac gcatcgccac gcgctacggc gtcgccggcg accgcctgga
      481 aagaacggac gacggctggc gcctgctcgc cgccgacggc agcgagatcg cccgcgcgcc
      541 ggtggtgatc ctggccgccg gcgccggcgc cacgcatttc gcgcagttcg ccggccagcc
      601 gcagaaaggc gcgcgcggcc agatcagcca cctgccggcc gccagcatca ccccgctgcc
      661 gcacgtcgtc tgccgccatg gctacctgat gccggagatc gacggcatct gcctgaccgg
      721 cgccacgctg caaccgggtg acgccgacct gaccttgcgc ccggctgacc acgccgaaaa
      781 cctggcccgc ctgcagcgca gcctgcccgg cttcgccacc gccatcgacc cggcgacgct
      841 cagcggccgc tgcggcgtcc gccccatgtc gcccgaccgc ttgcccatcg tcggcccgct
      901 gcccgacctc gccgcgctca agggccagcc gcgcctgccg gccctgcccc gccaccccgg
      961 cctgtggtgc gtgcagggtt tcggcgcgcg cggcatcgtc tggtcggcgc tgatggccga
     1021 cctgctgctg tcgcgcctgg aaggcgagcc gctaccgctg gagaacgacc tggtggacgc
     1081 cgtcgatccc ggccgcttcc tgctcaaacc ggccggccgg atgcaggaag acgacggctt
     1141 cgacgaccgc acgtgaaacc ggcataagct tagctgccaa tgttattggc agcccgccgg
     1201 acgcccggtt aagcttgccg ggctcctgta aacttcactt ttttccaaac acaaaagaga
     1261 gacacgcgga tgaagatcga aaccctcgcc gttcatggcg gctacagccc ggacccgacg
     1321 accaaggcag tcgccgtgcc gatctaccag accacctcct acgccttcga cagcacccag
     1381 cacggcgccg acctgttcga cctcaaggtc gccggcaaca tctacacgcg gatcatgaat
     1441 ccgacccagg acgtgctgga aaagcgcgtc gctgcgatgg aaggcggtgt tgccgcgctg
     1501 gccatggcct ccggcatggc ggcgatcacc gcctcgatcc agacgattgc cgaagccggc
     1561 gacaacatcg tcacctcgtc cacgctgtac ggcggcacct acaacctgtt cgcgcacacg
     1621 ctgccgcagt acggcatcga agtgcgcttc gccgactacc gcgacccgga tgccttcgaa
     1681 aagctgatcg acgccagaac caaggcagtg ttctgcgaat cggtcggcaa cccgctcggc
     1741 aacgtcgtcg atttcgagaa gctcgccgaa gtcgcccaca agcacggcgt gccggtcatc
     1801 gtcgacaaca cggtgccgtc gccctacctg tgccgcccgt tcgagcacgg cgccgacatc
     1861 gtcgtccacg cgctgaccaa gtacctgggc ggccatggca actcgatcgg cggcatcatc
     1921 gtcgatagcg gcaagttccc ctgggccgag cacaagcaga aattcaagcg cctcaacgag
     1981 ccggacgtct cctaccacgg cgtcgtctat accgaagcgc tcggcgccgc tgcctacatc
     2041 ggccgcgccc gcgttgtgcc gctgcgcaac atgggcgccg cgatcagccc gttcaacgcc
     2101 ttcctgatcc tgcaggggat cgagacgctg gcgctgcgca tggaccgcat ctgcgataac
     2161 tcgctgaaga ttgccgaatt cctcaagacg catccgaagg tcagctgggt caactacgcc
     2221 ggcctgcccg accacaagga ccacgcgctg gtccagaagt acatgggcgg ccgcgcttcc
     2281 ggcatcctca acttcggcgt caagggtggg cgcgaaggcg gcggcaagtt ccaggatgcc
     2341 ctgcagctgg tcacccgcct ggtcaatatc ggcgactgca agacactcgc ctgccacccg
     2401 gcgtcgacca cgcaccgcca gttgtcgccg gaagaactgg ccaaggccgg cgtctccgaa
     2461 gacatgatcc gtctgtcggt cggcatcgag cacgtcgatg acctgatcgc cgacctcgat
     2521 caggcgctca acgccgcctg agtcaccggc acccccgagc aagcccggca acgccgggct
     2581 ttttttgcgc ccaagcggcg ccaaatccac tcagccataa tgcattgaat tattcataac
     2641 acaatgattt taaatattta ttttgttttg ttgacctaaa tcagatggcg ctcgcatgcc
     2701 cccgtcgaaa atcgcccgga tcgtccaaac gatcgtttga cgcagtgcag caaaaaaatc
     2761 acaaatcaat tcgagggagc gcgtatgcaa cagcaagtca ctgaaaccaa aatcgtcatg
     2821 tccgacccca cggcgctggg tgttttcggt ttgtcgatgg tcaccttcgt cgccgcgtcg
     2881 gccaagatgg gctggaccac cggcgtcgcc tacctgattc catgggcgct gttcctcggc
     2941 tcgctggctc agatctgggc gtcgatggtc gacttcaaga agaacaacta tttcggcgcc
     3001 atcgtcctcg gcgcctacgg cctgttctgg atcgccgtcg ccatgcactg ggcaatcagc
     3061 ctcggctggc tgggcgaggt cggcgccaat gccgatccga agcaactcgc tttcgcctgc
     3121 atcg
//
LOCUS       DAOSDX010000277         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11043_length_3120_cov_2.613051, whole genome shotgun sequence.
ACCESSION   DAOSDX010000277 DAOSDX010000000
VERSION     DAOSDX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11043_length_3120_cov_2.613051"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLN02_07110"
     CDS             complement(<1..237)
                     /locus_tag="PLN02_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52133.1"
                     /translation="MPLSREQMLVEMGISPLWVLREPIPDNTSESPGAVAPTVIETPP
                     QSAAPVTENLARTETEPASTVAEFDWPELLAGIRD"
     gene            complement(228..662)
                     /gene="rimI"
                     /locus_tag="PLN02_07115"
     CDS             complement(228..662)
                     /gene="rimI"
                     /locus_tag="PLN02_07115"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOI52134.1"
                     /translation="MAVADLEAVTAVEASLQDFPWTRGNFADSLHAGYSAWIASIGGE
                     VIGFSVVMQVLDEAHLLNIGIASQHQGRGFGARLLRQAMTNAAQNGAHWMFLEVRPGN
                     ARAVALYRHFGFHEIGRRKDYYPARQGREDALVFQKELPACR"
     gene            complement(698..1390)
                     /gene="tsaB"
                     /locus_tag="PLN02_07120"
     CDS             complement(698..1390)
                     /gene="tsaB"
                     /locus_tag="PLN02_07120"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOI52135.1"
                     /translation="MQILALETSTEHGGCALWRDGDLLESACPDGRSHSETLLPLVRE
                     LLARAGTTVNALDGIAFGVGPGAFTGLRVACGAAQGLALAADLPLLPVTSLECMAAAS
                     GGTQVLALLDARMGEVYAAAYRNAVASPVLIDEIRVIAPAELAWPESGAGWLACGNAL
                     AAYPALAERLVSAGFSLQPDTLPLAAWVARLAAPRLLTGGGIDPALAAPLYIRDKVAK
                     TVAERLREGGRA"
     gene            complement(1422..1730)
                     /locus_tag="PLN02_07125"
     CDS             complement(1422..1730)
                     /locus_tag="PLN02_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52136.1"
                     /translation="MLRKMKIRLIVPLLAMLLLPAGPAAAGDHRYDFPSDDRRELRRQ
                     MRENWRQDAYDRRQQRDDPGQRWQEWPREERQRLRDEMRQQHERQDRDWRHERRHRRD
                     "
     gene            1881..2609
                     /gene="ompR"
                     /locus_tag="PLN02_07130"
     CDS             1881..2609
                     /gene="ompR"
                     /locus_tag="PLN02_07130"
                     /inference="COORDINATES: protein motif:HMM:NF007005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HOI52137.1"
                     /translation="MNENNQHILVVDDDARLRDLLTRYLGEQGFVVKAVGDAQQMEKT
                     RSREHYHLIVLDLMLPGEDGLSICRRLRGQGDRTPIIMLTAKGDEVDRIVGLEMGADD
                     YLPKPFNPRELLARIHAVMRRQGNVPPGAPEEALEAVRFGNIEVDFSARTLKRGDEIL
                     PLTTGEFAVLKVLLQHPRQPLSRDKLMTLARGREQGPFDRAIDVQVSRLRKLIENDPS
                     QPRYLQTVWGFGYVFMPDGAARES"
     gene            2609..>3120
                     /locus_tag="PLN02_07135"
     CDS             2609..>3120
                     /locus_tag="PLN02_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HOI52138.1"
                     /translation="MPRSLLARTFFLLALLVLSSTAGWLALFSHIDVEPRARESAAMA
                     TSAVNLIRASIFAAAPDKRPRLFAEFASTEGIRLLPAEDQDELVPMPDSRFHQLMRDT
                     IVARLGPTTRIAAEVNGEPGFWVSFRLDPKDEDEFWLVLPRERVTRDFATRWLSWAAL
                     AVSLALVVAW"
BASE COUNT          547 a         1100 c          950 g          523 t
ORIGIN      
        1 gtcgcgaatc cccgccagca gctccggcca gtcaaactcg gccaccgtac ttgccggttc
       61 tgtttctgtc cgcgccaggt tttccgttac cggagccgcc gactgcggcg gtgtttcgat
      121 caccgtcggc gcgaccgcgc caggcgactc tgaagtgttg tcgggaatcg gctcgcgcaa
      181 aacccacagc ggcgaaatcc ccatttcgac cagcatctgt tcacggctca gcggcatgcc
      241 ggcaactcct tttggaaaac cagcgcatcc tcgcgcccct ggcgcgccgg atagtagtcc
      301 ttgcgccgac cgatctcgtg gaaaccgaaa tgccgataca gcgccacggc gcgtgcatta
      361 cctggacgaa cctcgagaaa catccagtgc gccccgttct gcgcggcgtt cgtcatcgcc
      421 tggcgcagca ggcgtgctcc gaaaccgcgg ccctgatgtt gcgatgcgat cccgatattg
      481 agcagatgcg cttcgtcgag cacctgcatg actaccgaaa aaccgatcac ctcgccgcca
      541 atgctggcga tccaggcgct gtaaccggca tgcagcgagt cagcgaagtt gccccgcgtc
      601 cagggaaaat cctgcaacga ggcttcgacc gcggtgactg cctcgaggtc ggcaacggcc
      661 atcgggaaaa attccacggc aagtgccgcg acgccgttca cgcccgaccg ccttcgcgca
      721 ggcgctcggc gacggttttt gccaccttgt cgcgaatgta gaggggcgcc gccagcgccg
      781 gatcgatgcc gcccccggtc agcaggcgcg gcgccgccaa acgcgcaacc caggcggcca
      841 gcggcagcgt atccggttgt agcgaaaaac ccgcggacac caagcgttcg gccaacgccg
      901 gataggccgc cagggcattg ccgcaagcca gccagccagc accgctctcc ggccaggcca
      961 gttcggcggg cgcaatgacg cgaatctcgt cgatcaggac aggcgaagca acagcattcc
     1021 ggtaggcagc cgcataaacc tcgcccatgc gtgcatcgag cagcgccaga acctgcgtgc
     1081 ctccactggc tgccgccatg cactccaggc tggtcaccgg cagcagcggc agatcggccg
     1141 ccagcgccaa tccctgggca gcaccgcagg caacgcgcaa gccggtgaaa gcccccgggc
     1201 cgacgccgaa ggcgatgccg tcgagcgcat tgaccgtcgt tccagcccgg gcaagcagtt
     1261 cgcgcaccag cggcaacagg gtttctgagt gcgagcgccc atccgggcaa gcgctttcga
     1321 gcaagtcgcc gtcgcgccac agcgcgcagc caccgtgctc ggtcgaagtt tcaagggcca
     1381 ggatctgcat gggccgacat tctaccggag gcggccgaag ttcagtcgcg gcgatgtctc
     1441 cgctcgtgac gccagtcccg gtcctgcctt tcgtgctgct ggcgcatctc gtcgcgcaag
     1501 cgctgccgct cttcccgcgg ccactcctgc cagcgctgcc cgggatcgtc gcgctgctgc
     1561 cgacgatcat aagcatcctg gcgccagttc tcgcgcatct gccgacgcaa ctcccgccgg
     1621 tcgtcagagg gaaaatcata acgatggtcg ccggcagccg ctggccctgc cggcaacagc
     1681 agcatggcga gaagcggaac gatcaggcgg attttcatct tgcgcagcat tttatagcgt
     1741 tttccagacg caaccattgt ccgacttggg ccgccctaag gatcgatcga gttgtaatcg
     1801 aatgtttccc acgcccggcc gggatatcct ttgttaccat ttcccgcatt gccagccggc
     1861 gcacccctta tccttgccgc atgaacgaaa acaaccagca cattctcgtc gtcgatgacg
     1921 acgcccgcct gcgtgacctg ctgacccgtt acctcggcga acagggtttc gtcgtcaagg
     1981 cggtcggcga cgcccagcag atggaaaaga cgcgcagccg cgagcattac cacctgatcg
     2041 tcctcgacct catgctgccc ggtgaggacg gcctgtcgat ctgccgccgc ctgcgcggcc
     2101 agggcgaccg gacgccgatc atcatgctca ctgccaaggg cgacgaggtc gatcgtatcg
     2161 tcggcctcga aatgggcgcc gacgactacc tgcccaagcc tttcaatccg cgcgaactgc
     2221 tcgcccgcat ccacgccgtg atgcgccgcc agggtaacgt gccgccgggc gcgccggaag
     2281 aagcgctgga agccgtccgc ttcggcaaca tcgaagtcga tttctcggcc cgcacgctga
     2341 agcgcggcga cgaaatcctg ccgctgacca ccggcgaatt cgccgtcctc aaggtgctgc
     2401 tgcagcatcc gcgccagccg ctgtcgcgcg acaagctgat gaccctggcc cgcggccgcg
     2461 aacagggccc cttcgaccgc gccatcgacg tccaggtgtc gcgcctgcgc aagctgatcg
     2521 agaacgatcc ttcgcaaccg cgttacctgc agacggtctg gggcttcggc tacgtcttca
     2581 tgccggacgg cgccgcccgg gaaagctgat gccgcgctcg ctgctggcgc gcaccttctt
     2641 cctgctcgcc ttgctggtgc tatcgagcac cgccggctgg ctggcgctgt tcagccacat
     2701 cgacgtcgaa ccgcgggccc gcgaatcggc ggcgatggcg acttcggcgg tcaacctgat
     2761 ccgcgcctcg atcttcgccg ccgcgccgga caaacgtccg cgcctgttcg ccgaattcgc
     2821 ctcgaccgaa ggcatccgcc tgctccccgc cgaagatcag gacgaattgg tgccgatgcc
     2881 ggattcgcgt tttcaccagt tgatgcggga cacgatcgtc gcccgcctcg gtccgacgac
     2941 gcggatcgcc gccgaggtca atggcgaacc cggcttctgg gtcagcttcc gccttgaccc
     3001 caaggacgag gacgagttct ggctggttct gccacgcgag cgggtgacac gcgatttcgc
     3061 gacgcgctgg ctgagttggg cggcgctggc ggtaagtctg gcgctggtcg tcgcctggtt
//
LOCUS       DAOSDX010000278         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11065_length_3117_cov_2.618550, whole genome shotgun sequence.
ACCESSION   DAOSDX010000278 DAOSDX010000000
VERSION     DAOSDX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11065_length_3117_cov_2.618550"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLN02_07140"
     CDS             <1..409
                     /locus_tag="PLN02_07140"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOI52139.1"
                     /translation="KEEWKGGAECNKILGKPAFRTAGSIPIDGLCVRIGAMRCHSQAL
                     TIKLKKDVPLDEISDMLAKANPWAKVVPNDREISERELTPAAVTGTLTVPVGRLHKMA
                     MGPEYLAAFTCGDQLLWGAAEPLRRMLRILLDA"
     gene            628..>3117
                     /locus_tag="PLN02_07145"
     CDS             628..>3117
                     /locus_tag="PLN02_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOI52140.1"
                     /translation="MSLATSLAEAAGLGKLTVFSSLGQPLKAEVEIAASSEELSGMTA
                     RLAPPEVFKQAGVDFASALSDLQFKVEKRRNGKTVLRLTSVHPVNEPFLDFLVELNWP
                     AGRLVREYTFLLDPPEFAAQQGQRNTVDAKVVETVRGGAAAAPKPAAPAKPKVEEKPA
                     EGSATKTGDDKTHLVAKGDTLVKIARERRYASVSLEQMLVGLYRGNSDAFIGKDINRL
                     KSGVILNVPEESVVAAVSQKEARAVYVSAGDFAAYRRKLAAAAVAAPATKAEAPAGQS
                     SGGKITPKIEEKAPVVDATRDQVKVSRADAPSASVIDRVASEKAMQESQTRLAQLERN
                     IGELQKLLELKNQRLAELEKPAAVAAPAPAPAAKPEAAATKPEPTPPVAEPAAAPTPP
                     LASEGGARVIDANAPAPVEEKPAEPPAQVTPPPAPPKPVAPPPEPPEEPSELEGAMPL
                     LGGAAVLALLGAGWLYSRRRKSAAGPVTQVTAVPAPSSLGPNSVFGVTGGQSIDTGST
                     PPHTGDFSQAGPGTIDTDEVDPVAEADVYMAYGRDAQAEEILLEAMQKDPNRLAIHVK
                     LLEIYAGRRSLKQFETLASELYALTSGQGPEWEKVAALGAGIDPGNPLYGGAAKEKGF
                     DPDATLVASSAAVAAAAAAAAQPAVAEVVVPAIECEEAAPAVSAVPVSEPAVVPMVES
                     LVAEPAMPEDDGGMSLDFDLGDTSTETVTPPEEDVPAPAVEPYVDTVVTGENVADALD
                     FDLSGLVADEAAAAETPAHAGEAADTEDLVVDFDSIDLPDSADFDIAAPMEEAAAPDF
                     SPEGTLVVSGTGLGQMSASDFSFDALRASEPA"
BASE COUNT          582 a          957 c         1032 g          546 t
ORIGIN      
        1 gaaggaggaa tggaagggcg gcgccgagtg caacaagatc ctcggcaagc cggctttccg
       61 tacggcgggt tcgattccca tcgacggcct gtgcgtgcgc atcggcgcga tgcgctgcca
      121 tagtcaggcg ctgaccatca agctgaagaa ggacgtgccg ctcgacgaaa tcagcgacat
      181 gctggccaag gccaatccgt gggccaaggt cgtgccgaat gatcgcgaga tttccgagcg
      241 cgaactgacg ccggctgccg tcaccggcac gctgacggtg ccggtcggcc gtctgcacaa
      301 gatggcgatg ggcccggaat acctggccgc cttcacctgc ggcgaccagt tgctgtgggg
      361 ggccgccgag ccgctgcgcc gcatgctgcg catcctgctc gacgcctgat atggcatgaa
      421 ttgccaaaaa acggggcttt tgccccgttt ttttatttgt tggctatacg caaaaagtcc
      481 gtaactcaga agcgggttta gcttgcaatg ctaagtccat gatgttaatt tgattctccc
      541 aagtcatacg ctcagggcat ggtcgtgatc gagaataatt ctgtcaaatt caagaaatgt
      601 gcgctggctt tggccgtcgc ttcgtcattg tcgctcgcga cctcgcttgc cgaagccgcc
      661 ggactcggga agctgacggt cttttccagt ctggggcaac cgctgaaggc cgaggtggaa
      721 atcgccgcga gcagcgaaga actgtctggg atgacggcgc gcctggcgcc tcccgaagtg
      781 ttcaagcagg ccggcgtcga ctttgcttca gccctgagcg acctgcagtt caaagtggaa
      841 aaacggcgta acggcaagac cgtgttgcgc ctgacttcgg tccacccggt caacgaaccc
      901 ttcctcgact ttctggtcga gctgaactgg ccggctggac gtctggtccg tgaatatact
      961 ttcctgctcg acccgcccga attcgcggcg cagcaggggc agcgcaacac ggttgatgcc
     1021 aaggtggtgg agaccgtccg tggtggagcg gcagccgcgc cgaagccggc ggcgccagcc
     1081 aagcccaagg tcgaagagaa acccgcagaa ggcagtgcga ccaagactgg cgatgacaag
     1141 acccatctcg tcgccaaggg cgatacgctg gtcaagatcg cccgtgaacg ccgttacgca
     1201 tcggtatcgc tcgagcagat gctggtcggc ctctatcgcg gcaacagcga cgccttcatt
     1261 ggcaaggaca tcaatcgcct gaagtcgggc gtcatcctca atgtgcccga ggagagcgtg
     1321 gttgctgcgg tctcgcagaa ggaagcgcgg gcggtgtacg tctccgccgg cgattttgct
     1381 gcctatcggc gcaagttggc ggcggcagcc gtcgcagcgc cggccaccaa agcggaagcg
     1441 cctgccgggc aaagcagcgg cggcaagatc acgcccaaga tcgaggaaaa ggcgccggtt
     1501 gtcgatgcga cgcgggatca ggtcaaagtc tcccgggcag acgcgccctc ggcctcggtg
     1561 attgatcgcg tcgcctcgga aaaggcgatg caggagtctc agacacgctt ggcgcaactg
     1621 gagcgcaata tcggcgaact gcagaagctc ctcgaactga agaaccagcg tctggccgaa
     1681 ctggaaaaac ctgccgcggt tgcggcgccg gctccggcgc cggccgccaa accggaagcg
     1741 gcggcgacca aacccgaacc gacaccgccg gttgctgaac ccgccgctgc gccgaccccg
     1801 ccgcttgcca gcgaaggtgg tgcgcgagtc atcgacgcca acgctccggc gcccgtcgag
     1861 gagaagccag ccgagccgcc ggcgcaggtg acgccaccgc cggcgccgcc gaagccggtt
     1921 gctcctccgc cggaaccgcc cgaagagccc agcgaactgg aaggcgcgat gcccctgctc
     1981 ggtggcgccg ccgttctcgc cttgctcggt gccggctggt tgtacagccg tcggcgcaag
     2041 agcgccgccg ggccggtcac tcaggtgacc gccgtgccgg ctccatccag tctcggtccc
     2101 aattcggtat tcggggtgac tggcggacag agcatcgata ccggcagcac gccgccgcat
     2161 accggtgatt tcagccaggc gggaccgggg accattgata ccgacgaggt cgatccggtt
     2221 gccgaagccg atgtctacat ggcctatggt cgcgatgcgc aggctgagga aatcctgctc
     2281 gaagcgatgc agaaggatcc caatcgtctg gcgattcacg tcaagctact cgagatttat
     2341 gccggtcggc gcagcctcaa gcaattcgag acgctggcca gcgagcttta cgcactgacc
     2401 agtggacagg ggccggaatg ggaaaaggtt gccgcgctcg gcgccggtat cgacccgggc
     2461 aaccccttgt acggcggtgc ggcgaaggaa aaggggtttg atccggatgc gacgctggta
     2521 gcctcttcgg cggctgttgc cgcggctgcg gccgcagccg cgcagcctgc tgtcgccgag
     2581 gtcgtggtgc cggccatcga gtgcgaggaa gcggcgccgg cagtcagcgc cgtgccggtt
     2641 tccgaacccg ccgtggtgcc gatggtcgag tccttggtcg ctgagccggc gatgccggaa
     2701 gatgatggcg ggatgagtct ggatttcgat ctgggcgata cttcgacgga aaccgtgacg
     2761 ccgccggagg aggatgttcc ggctccggcc gttgagcctt atgtcgatac cgtggtgact
     2821 ggcgagaacg ttgccgatgc gctcgatttc gacctcagcg gtctggttgc cgatgaggcc
     2881 gcggctgcgg aaaccccagc ccacgccgga gaagccgctg acacggaaga cttggttgtc
     2941 gatttcgata gcatcgatct gccggacagt gccgatttcg atatcgctgc tccgatggaa
     3001 gaagccgctg cccccgactt ttcgcccgaa ggtacgctgg ttgtttccgg taccggcctg
     3061 ggtcagatgt cggccagtga tttctccttt gacgcgctgc gggcttcgga accggcc
//
LOCUS       DAOSDX010000279         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11073_length_3115_cov_3.896078, whole genome shotgun sequence.
ACCESSION   DAOSDX010000279 DAOSDX010000000
VERSION     DAOSDX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11073_length_3115_cov_3.896078"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PLN02_07150"
     CDS             <1..1416
                     /locus_tag="PLN02_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOI52141.1"
                     /translation="SLTPLLKLPPPPVPANFPLSTKPAASAVPASQLGWRAMFGDPRL
                     QRLIELALQHNRDLRLAALNVQAAKEMAKVQRAGLLPAVGLAATQTRERALATDGGGR
                     TTRSVSRQAEVNVAVSAFELDLFGRVRALSDAALARYLASEHGRDAVHIALVSAVAEA
                     YFAQQLAVEQRQLAERMLADWQQSLALARLLKQAEQSSSLDVAQAEAQAALAEAELEA
                     RRRAEAQAANALQLLVGGELPGDLPAALPLAGQPVIARLPAGLPSELLLTRPDLRQAE
                     QMLVAANADIGAARAAFFPQISLTASFGYASTALGGLFDPGHQVWRFAPQLSQPLFQG
                     GRLRAELRLAEVRKSEAVAQYERAIQNAFREVADALAGTATFDRQIAAQQRALDAAER
                     RLALSRQRYQAGFDGRLELLDAQRQFDAARQAMLALRHEEIVNRVALYKSLGGGLGAH
                     AGSSDAFVATPAALNPGAGAS"
     gene            complement(1504..2145)
                     /locus_tag="PLN02_07155"
     CDS             complement(1504..2145)
                     /locus_tag="PLN02_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52142.1"
                     /translation="MKRLLPLILVAACAAVPGKPGEREIFNAGLRQAWANEAQTMSAV
                     RPPLRFANGRTVRDCHGYLAEHASGTVDEAVNNRGIAQAYLVCDALAQLDQAKALAPA
                     AALPPSYGRELLQRLDLRSFPSSLRPRLGEKFTLAAIGGAVPQIETHAVTLETEDWRY
                     RFEVIADIVAADGRREWLVWFSDEAKAGNYRGYATLRLPVAAARGLITAIAAP"
     gene            complement(2142..2705)
                     /locus_tag="PLN02_07160"
     CDS             complement(2142..2705)
                     /locus_tag="PLN02_07160"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF028064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin C-terminus-like muramidase"
                     /protein_id="HOI52143.1"
                     /translation="MANQIDYKFISDREGGSVTEAYVPASEVSKSGVTVATGFDLGQR
                     NESDLNNLGLSAALVTKLKPYLGKTAKAATDALSARSLTISAAEATEIDKAVKAKEVD
                     KLKRRYAAAIDNSAKTDFYSLPAEAQTVIASVSFQYGDLSAKTPKFWKAVSAQDWAEA
                     VKLLRAFGDAYPTRRGLEATLLEKIVK"
BASE COUNT          428 a         1046 c         1090 g          551 t
ORIGIN      
        1 tcgctgacgc ctttgctgaa gctgccgccc ccgcccgtgc cggcgaattt cccgctgtcg
       61 acgaagcctg ccgcgtcggc cgtgccggcc agccagctcg gctggcgcgc gatgttcggc
      121 gatccgcgtc tgcaacggct gatcgagctg gccctgcagc acaatcgcga tctgcgcctg
      181 gccgcgctca acgtccaggc ggcaaaggaa atggcgaagg tccagcgcgc cggactgttg
      241 cccgccgtcg gcctggccgc gacgcagacg cgcgagcggg ccctggcgac cgatggcggc
      301 ggccgcacga cccgctcggt gagccggcag gccgaagtga atgtcgcggt cagcgccttc
      361 gaactcgatc tcttcgggcg cgtgcgggcg ctctccgatg cggcgctggc gcgctacctg
      421 gcgagcgaac atgggcgcga cgcggttcat atcgcgctcg tcagcgccgt cgccgaggcc
      481 tacttcgcgc agcagctggc cgtcgagcaa cggcagctgg cggaacgcat gctggccgat
      541 tggcaacagt cgctggccct ggcccgcctg ctcaagcagg ccgaacagag cagcagcctc
      601 gatgtcgccc aggccgaggc ccaggcggcg ctggccgagg ccgaactgga agcgcgccgg
      661 cgtgccgagg cgcaggcggc gaatgcgctg cagttgctgg tcggcggcga actgcccggc
      721 gacttgccgg ccgccctgcc gctcgccggg cagccggtga tcgcccggct gccggccggc
      781 ttgccgtcgg agctgctatt gacccggccc gatctacgcc aggccgaaca gatgctggtg
      841 gcggccaacg ccgatatcgg cgcggcgcgg gccgcgttct ttccgcagat ttcgctgacc
      901 gcgtcgttcg gttacgccag taccgcgctt ggcggcttgt tcgatccggg acaccaggtc
      961 tggcgtttcg ccccgcaact gtcgcagccg ctgttccagg gcggccgttt gcgcgccgaa
     1021 ctgcgcctgg ccgaggtccg caagtccgag gccgtcgccc agtacgaacg cgccatccag
     1081 aacgccttcc gcgaagtcgc cgatgccctc gccggcaccg ccaccttcga tcgccagatc
     1141 gccgcgcagc aacgggcgct ggacgccgcc gagcgtcgcc tggccttgtc ccggcaacgc
     1201 taccaggccg gcttcgacgg ccgtctcgaa ctgctcgacg cgcaacgcca gttcgacgcc
     1261 gcgcggcagg caatgctggc attgcgccac gaggaaatcg tcaaccgggt ggcgctctac
     1321 aaatccctcg gcggtggcct gggcgcccac gcggggagta gcgacgcctt cgtcgcaacg
     1381 cccgcagcac tcaatccggg tgctggggcg tcatgatgtc tggtacggcg attggtgcag
     1441 cttgccggcc acgggtggct gggaggattc cccgtttgcg gactgatgcg cggcgcgagc
     1501 ggatcaaggc gcggcgattg ccgtgatcaa tccccgcgcc gccgccaccg gcaggcgcag
     1561 cgtcgcgtag ccgcgatagt tgccggcctt ggcctcgtcg ctgaaccaga ccagccattc
     1621 gcgtcgaccg tcggcggcga cgatgtcggc gatgacctcg aagcggtagc gccagtcttc
     1681 agtctccagc gtcaccgcgt gcgtttcgat ctgcggcacg gcaccaccga tggcggcgag
     1741 cgtgaacttc tcgcccaggc gcggtcgcag cgacgacggg aagctgcgca ggtccaggcg
     1801 ctgcagcagt tcgcggccgt aggacggcgg cagcgccgcc gccggcgcca gtgccttggc
     1861 ctggtcgagc tgggccagcg cgtcgcacac caggtaggcc tgggcgatgc cgcggttatt
     1921 gaccgcttcg tcgacggtgc cgctggcgtg ctcggccagg tagccgtgac aatcgcgtac
     1981 cgtgcggccg ttggcgaagc gcagcggcgg ccggacggcg ctcatcgtct gcgcttcgtt
     2041 cgcccaggcc tggcgcaggc cggcgttgaa gatttcgcgc tcgccgggct tgcccggcac
     2101 ggcggcgcag gcggcaacga ggatgagggg aaggaggcgt ttcacttgac gattttctcc
     2161 agcagggtgg cttccagtcc gcgccgcgtc gggtaggcat cgccgaaagc ccgcagcagc
     2221 ttgacggctt cggcccagtc ctgcgcactg acggctttcc agaatttcgg ggtcttggcg
     2281 ctgaggtcgc cgtactggaa ggataccgag gcaatgacgg tttgtgcttc ggccggcagc
     2341 gaatagaagt cggttttcgc gctgttgtcg atggcggccg cgtagcggcg cttcagctta
     2401 tcgacttcct tggccttcac cgccttgtcg atctcagtcg cctccgccgc cgagatggtc
     2461 aggctccggg cgctcagcgc atcggtcgcc gccttggcgg tcttgccgag atagggcttg
     2521 agcttggtga ccagcgcggc ggacaggccg aggttgttca agtcggactc gttgcgctgg
     2581 ccaaggtcga aacccgtcgc caccgtcacc ccgctcttgc tcacctcgct tgccggaacg
     2641 taggcctccg tgacgctgcc gccttcgcgg tcgctgatga atttgtagtc gatctggttt
     2701 gccatgtcct gtccttgtgc cggtgagctt gaattgatag gtcgctattc taggttgaag
     2761 ccatcggcat atgacaaaaa gtttttgtcg atggggtctc acggcgctcg tcgacacaag
     2821 ccggttgaac gtcctttgtg tccgattccg tccgcttctg gtcccgttcc ggtctgtcgt
     2881 gggttccggc agccgcctaa gcttcatctc cagggaaacc gtcacgggag acgatcatgg
     2941 aacgcgaagt cctgctgttg ttgccgctgc cggccggggt cgatggcgat tcggcattgg
     3001 tccgctgctt gttcgagcac tggtggcgag gcgcggcggc gctggcgctg ggtggtgcgg
     3061 atggctggcc ggtgccggtc gagcggcgct tcgccggggc cggcgtgccg ctggc
//
LOCUS       DAOSDX010000280         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11100_length_3110_cov_2.872340, whole genome shotgun sequence.
ACCESSION   DAOSDX010000280 DAOSDX010000000
VERSION     DAOSDX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11100_length_3110_cov_2.872340"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLN02_07165"
     CDS             <1..303
                     /locus_tag="PLN02_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52144.1"
                     /translation="RRSIEEGMHHLETSQAAVDSVSSVLQAANGSVAEVGHGLDTIAQ
                     ATDQQRRVSGDVESSIEAIAGMARDNTGTVEQTAGAAHDLRRLAEGLANLVGRFKV"
     gene            complement(349..1707)
                     /locus_tag="PLN02_07170"
     CDS             complement(349..1707)
                     /locus_tag="PLN02_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOI52145.1"
                     /translation="METIGKYRVIRELGKGATATVYLCDDPENARQIAVKVIRFGQDC
                     AAMSRRMRKLFQNEGMVSRRLDHPNIVRVYDAVVEDEFSYLAMEYVDGFSLEQHCRID
                     KLLPLHRVVSIIFKCCMALDAAYRQGIIHRDIKPANIMVAANDEPKIADFGLALNLNK
                     DLDRDSTFIMGVGSPAYMSPEQIKGYPLNQKTDLYSLGVVMFQLLTGRLPFRANNQAT
                     LVYKIVNTDPPAVSALNPALPEGLDAILRKALEKDLYSRYRNGAEMAKDLSAVRYKIL
                     EEDETEHDMRHFEQLRRVEFFTEFENIELWEVLRVSLWRQVTANVRLITEGSNSRLFG
                     IILAGSVEVSLGGKALCRLGVGEPVGEVAFLHPSSDLRHATVVTLEPTLFLEVNASAL
                     ALSSEELMGRMRDALLARMIYRLREVNKIAASIGQPAVNNASGHYGGKSCGGVTLELA
                     PE"
     gene            1861..2415
                     /gene="grpE"
                     /locus_tag="PLN02_07175"
     CDS             1861..2415
                     /gene="grpE"
                     /locus_tag="PLN02_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOI52146.1"
                     /translation="MSNEETPQDHLLDAAQAETPASEPEKERHIDVLPSLEEQIKALE
                     LKVAETHDAWLRAKAEGENIRRRAQEDISKAHKFAVEKFAGELLAVKDSLEAALAVPE
                     QTVESFKSGVELTLKQLVAAFEKNALNEVNPVGEKFDPHKHQAIGMVDSEQEANTVVT
                     VLQKGYLIADRVLRPALVMVAKGK"
     gene            2485..>3110
                     /locus_tag="PLN02_07180"
     CDS             2485..>3110
                     /locus_tag="PLN02_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOI52147.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGTPKVIENAEGARTTPSVIGYTD
                     DGEILCGAPAKRQAVTNPKNTLYAVKRLIGRRFEEKEVQKDIALMPFKIVKADNGDAW
                     VEVRDKKMAPPQVSAEVLRKMKKAAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRV
                     AGLEVKRIINEPTAAALAFGMDKKEGDRKIAVYDLGGGTFDISIIEIA"
BASE COUNT          670 a          985 c          940 g          515 t
ORIGIN      
        1 cggcgcagca tcgaggaagg catgcaccac ctcgaaacca gccaggctgc cgtcgactcc
       61 gtttccagcg tgctgcaggc cgccaacggc tcggtcgccg aagtcggcca cgggctcgat
      121 accattgctc aggccaccga ccagcagcga cgcgtgtccg gcgatgtcga atcgagcatc
      181 gaagcgatcg ccggcatggc ccgcgacaac accggtaccg tcgaacagac cgccggcgcc
      241 gcccatgacc tgcgccgcct ggccgaggga ttggccaatc tggtgggccg cttcaaggtc
      301 tgagcgcacc accgcgccaa ccacaacgcc gcccgcgggc ggcgttattc attccggcgc
      361 cagctccagc gtcaccccgc cgcatgactt gccgccgtaa tgcccgctgg cgttattgac
      421 ggcgggctgg ccgatgctgg cggcgatctt gttgacctca cgcaggcgat aaatcatgcg
      481 agcgagcagc gcatcgcgca tgcggcccat cagctcttcc gacgacaaag ccagcgccga
      541 tgcattgacc tcaaggaaca gggtcggctc cagcgtcacc accgtcgcgt gacgcaggtc
      601 gctcgacgga tgcaggaagg ccacctcgcc gaccggctcg ccaacgccaa gacggcatag
      661 tgccttgccg cccaacgaaa cctcgacgct accggcaagg atgatgccga acaggcggct
      721 gttgctgccc tcggtgatca agcggacatt ggccgtcacc tgccgccaca gcgagacgcg
      781 cagaacttcc cagagttcga tgttctcgaa ttcggtgaag aactcgacgc gacgtaactg
      841 ctcgaagtgc cgcatgtcgt gctcggtctc gtcctcttcc aggatcttgt agcgcaccgc
      901 cgaaagatcc ttggccattt ccgcgccgtt gcggtaacgg ctgtacaggt ccttttccag
      961 cgccttgcgc aggatcgcgt cgagcccttc gggaagcgcc ggattgagcg cgctgaccgc
     1021 cggcggatcg gtattgacga tcttgtacac cagcgtcgcc tggttgttgg cgcggaaagg
     1081 cagacgcccg gtcagcagct ggaacatgac gacgccgagc gaatagaggt cggtcttctg
     1141 gttgagcggg tagcccttga tctgttccgg cgacatgtag gccggcgaac cgacccccat
     1201 gatgaaggtc gagtcgcggt cgagatcctt gttcaggttc agcgccagcc cgaaatcggc
     1261 gatcttcggc tcgtcgttgg cggcaaccat gatgttggcc ggcttgatgt cgcggtggat
     1321 gatgccctgc cggtaagcag cgtcaagggc catgcagcac ttgaagatga tgctgaccac
     1381 ccggtgcagc ggcagcagct tgtcgatccg gcaatgctgc tcaaggctga agccgtccac
     1441 atactccatc gccaggtagg agaattcgtc ctcgaccact gcgtcataga cccggacgat
     1501 gttcggatga tcgagccgcc gcgagaccat cccctcattc tgaaagagct tgcgcatgcg
     1561 ccgtgacatg gcggcgcaat cctggccgaa acggatcacc ttgacggcaa tctgccgcgc
     1621 attttccggg tcgtcacaaa gatagaccgt agcggtcgct cccttgccga gctctcggat
     1681 gacgcggtat ttgcctatcg tttccatgac attgacgttg aatgaatacc tccttggcga
     1741 tgctagcacg ccgacgcgcg caacaaagca aacaaatgtg tagtaaaaaa ttttcgagcg
     1801 gctcttgaaa tgtttttttc catccccatc tccccgttcg gtttattgga gaaaatgcgc
     1861 atgtctaacg aggaaacccc gcaggaccac ctgctcgatg ccgcccaggc ggaaacgccg
     1921 gccagcgaac cggaaaagga acgccacatc gacgttctgc cgagcctgga agaacagatc
     1981 aaggcgctgg aactgaaggt cgccgaaacc catgacgcct ggctgcgcgc caaggccgaa
     2041 ggcgaaaaca tccgccgccg cgctcaagaa gacatcagca aggcgcacaa gttcgcggtc
     2101 gagaaattcg ccggtgaact gctcgccgtc aaggacagcc tggaagcggc gctggcggtg
     2161 ccggagcaga cggtcgagag tttcaagtcc ggcgtcgagc tgaccttgaa gcaactggtt
     2221 gcggcttttg agaagaacgc gctgaacgag gtcaacccgg tcggcgaaaa attcgacccg
     2281 cacaagcacc aggcgatcgg catggttgat tccgaacagg aagcgaacac cgtcgtcacc
     2341 gtactgcaga agggctacct gatcgccgac cgcgtgctgc ggccggcgct ggtcatggtc
     2401 gccaagggca aataatccac ttgaaatccg gggcaatgcc cccacttcac gaacctcgcc
     2461 aattcacttt caggaaagaa catcatgggc aagatcatcg gtatcgacct cggcaccacc
     2521 aacagctgcg tcgccatcat ggaaggcggc acgccgaagg tcatcgaaaa cgcggaaggc
     2581 gcacgcacca cgccgtcggt catcggctac accgacgacg gcgagatcct ctgtggcgcc
     2641 ccggccaagc gccaggccgt gaccaacccg aagaacacgc tgtacgcggt caagcgcctg
     2701 atcggccgcc gcttcgaaga gaaggaagta cagaaggaca tcgccctgat gcccttcaag
     2761 atcgtcaagg ccgacaacgg cgacgcctgg gtggaagtgc gcgacaagaa aatggcgccg
     2821 ccgcaggtgt cggccgaggt gctgcgcaag atgaagaagg ccgccgagga ctacctgggt
     2881 gaggaagtca ccgaggcggt catcaccgtg cccgcctact tcaacgacag ccagcgccag
     2941 gcgaccaagg acgccggccg cgtcgcgggc ctggaagtga agcgcatcat caacgagccg
     3001 accgccgcgg cgctcgcctt cggcatggac aagaaggaag gcgaccgcaa gatcgcggtg
     3061 tacgacctgg gcggcggcac cttcgacatt tcgattattg aaattgccga
//
LOCUS       DAOSDX010000281         3104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11122_length_3104_cov_3.377829, whole genome shotgun sequence.
ACCESSION   DAOSDX010000281 DAOSDX010000000
VERSION     DAOSDX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11122_length_3104_cov_3.377829"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLN02_07185"
     CDS             complement(<1..244)
                     /locus_tag="PLN02_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOI52148.1"
                     /translation="MHRGHAALIGRLAEVAGERSLVPTVLSFEPHPREFFAPDSAPAR
                     LSTFREKLELLAASGVAQAMICPFNRAFAALGAQAFI"
     gene            445..1488
                     /locus_tag="PLN02_07190"
     CDS             445..1488
                     /locus_tag="PLN02_07190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOI52149.1"
                     /translation="MLSLINHVLRISARRDRTEINSAMVDALKELFSPRRLTIYRCYS
                     SKRKTIVFACAGYGPQGQFLRNAYLPEARFCQPIERDPLLRQCRRELSAVLEPMGDGS
                     HRVIFPILRLDEPIYFIDLVMEEDFSADLRVTLMGLVEYFGNHIGLLDYGEADTLTGL
                     ASRKTFDKHLFELLGLAASDHMLGNAPQPARRNEQADSNHWLAVCDIDHFKRVNDSYG
                     HLIGDEVLIMMSQVMRQSFRFDDQLFRFGGEEFIALLQPTNRQSAAATLERFRHDVEQ
                     TVFSRVGHVTVSIGFSRLLLSDTPSDVIERADEALYYAKRNGRNQLACYEQLIEDGRL
                     AAREIAKGEVELF"
     gene            complement(1493..2203)
                     /locus_tag="PLN02_07195"
     CDS             complement(1493..2203)
                     /locus_tag="PLN02_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52150.1"
                     /translation="MQAAPLTELRRLYTAADIAGDAAQLPLLDAAGTARALLIEFPQA
                     SDWPSVGATLSYLADELALPQPALSVSVGGAFTLWLAFATPRPLPQLAAFYDGLRAAC
                     LSDLPAERVRCHPLSGVEAVPLVPAFDAGVERWSAFIDPGMGSMFVSEPGLEFPPNPE
                     RQAEMLAGLRAIDDRQLAKAMAQLPTPVQAPAAGALPLAGHFADPAAFLLAVMNDASV
                     PLGERISAASALLAAGRR"
     gene            complement(2203..>3104)
                     /locus_tag="PLN02_07200"
     CDS             complement(2203..>3104)
                     /locus_tag="PLN02_07200"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOI52151.1"
                     /translation="RLVLDIYPETPADPLMALLEGRRDAVEPLKPGQDLGGEEAKPAK
                     APEPQVAKRSGKAVVDRLVTIMLDPGHGGEDPGAVGKRGTHEKAVTLAIARRLKARID
                     AEPNMRAVLTRDGDFFVPLQVRVQKARRIQSDLFLSIHADAWIKPDARGSSVFVLSEK
                     GASSTQARLLAQKENSADLIGGVNLAKTKDVFLARTLLDLSQTATINDSLKLGKYLLG
                     ELGSINHLHKTSVEQAGFAVLKAPDIPSALIETAFISNPEEELRLKDDAYQEQMADAI
                     MRGIRQYFIKHPPGPKAKIAQIG"
BASE COUNT          574 a         1025 c          979 g          526 t
ORIGIN      
        1 cgatgaaggc ttgcgccccc agcgccgcga aagcgcggtt gaaggggcag atcatcgctt
       61 gcgccacgcc gctggcggcg agcagttcga gcttttcgcg gaaggtgctg aggcgggccg
      121 gtgccgaatc gggcgcgaag aactcgcgcg gatgcggctc gaagctcaat accgtcggca
      181 ccagcgagcg ctctccggcc acctcggcca gacggccgat cagcgcggcg tgtccacgat
      241 gcacgccatc gaaattgcca atggccagtg cgaccggtgc cggaacagga cgagaataac
      301 cgcgaaaaac gcgcatcgga tgctgctgca aaaaagcgcg attataccgg ggtgccctga
      361 agttccggcc aaatccacga aaaaacagcc ccttgccgca gaggccgccg ctataatctg
      421 gcaaaatcga caagccatcc catgatgctc agcctgatca accatgttct tcgcatttcg
      481 gcgcgccgcg accgtaccga aatcaactcg gcaatggtcg acgcactgaa ggagctgttt
      541 tccccacgcc ggctgaccat ttatcgctgc tattccagca aacgcaagac cattgtcttc
      601 gcctgcgccg gctatggccc gcaagggcag ttcctgcgca acgcctacct gcccgaagca
      661 cgcttctgcc agccgatcga acgcgacccg ctgctccgac aatgccggcg cgaactctcc
      721 gccgtcctcg aacccatggg cgacggttcg catcgggtga tcttcccgat attgcgcctt
      781 gatgaaccga tctacttcat tgatctggtg atggaagagg atttttccgc cgacctgcgc
      841 gtcaccctga tgggactggt tgagtatttc ggcaaccaca tcggcctcct cgactacggc
      901 gaggccgaca cgctgaccgg cctggccagc cgcaagactt tcgacaagca cctgttcgaa
      961 ctgctcggtc tggccgcttc cgaccacatg ctcggcaacg cgccgcaacc tgcccggcgc
     1021 aacgaacaag cggacagcaa ccactggctg gcagtctgcg acatcgacca tttcaagcgg
     1081 gtcaatgaca gctacggcca cctgatcggt gacgaggtgt tgatcatgat gtcgcaggtg
     1141 atgcggcaga gttttcgttt cgacgaccag ttgttccgct tcggcggcga ggaattcatc
     1201 gccctgctgc agccgaccaa ccgccagtcg gccgcggcaa ccctggagcg cttccgccac
     1261 gacgtcgagc agacggtttt cagccgcgtc ggccacgtca ccgtcagcat cggcttcagc
     1321 cgcctgctgc tcagtgacac cccgagcgac gtcatcgaac gcgccgacga ggcgctttac
     1381 tatgccaagc gcaacggccg caaccagctt gcctgctacg agcagttgat cgaagacggg
     1441 cggctggccg cccgcgaaat cgccaagggc gaagtcgaac tcttctgagg cctcagcggc
     1501 ggccagcggc gagcaaggcg ctggccgcgc tgatccgctc gccgagcggc acggaagcgt
     1561 cgttcatcac cgccagcagg aaagccgccg gatcggcaaa atgtccggcc aagggcaaag
     1621 cacctgcagc cggcgcctga accggtgtcg gcagttgggc cattgccttg gccaactgcc
     1681 ggtcgtcgat cgccctcaag ccggccagca tttccgcctg ccgctccgga ttgggcggaa
     1741 actccagccc cggctcgctg acgaacatgc tgcccatgcc gggatcgatg aaggcagacc
     1801 agcgctcgac accggcgtcg aacgctggca ccaacggcac tgcttcaacc ccgctcaacg
     1861 gatgacagcg cacccgctcg gccggcagat cgctcaggca agctgcccgc aggccatcgt
     1921 aaaacgcggc cagttgtggc agtggccgcg gtgttgcgaa agccagccag agcgtgaacg
     1981 cgccgccgac ggacaccgac agcgctggct gcggcaaggc cagctcgtcg gcgagataac
     2041 tcagggttgc accaacgctc ggccagtcgc tggcctgcgg gaattcgatg agcaaggcgc
     2101 gggccgtgcc agcggcgtca agcagcggca gttgcgcggc atcgccagcg atatcggcag
     2161 ccgtgtaaag acggcgcagt tcagtgagcg gcgcggcctg catcagccga tctgggcgat
     2221 cttcgccttc ggccccggcg gatgcttgat gaagtactgg cgaatgccgc gcatgatggc
     2281 gtcggccatc tgctcctgat aggcgtcgtc cttcaagcgc agctcttctt ccggattgga
     2341 gatgaaggcg gtctcgatca gtgccgatgg aatgtccggc gccttgagga cggcgaatcc
     2401 cgcctgctcg acgctggtct tgtgcaggtg gttgatcgag ccgagttcgc cgagcaggta
     2461 cttgccgagt ttgaggctgt cgttgatggt cgccgtctgc gacagatcga gcagggtgcg
     2521 ggccaggaaa acgtccttgg tcttggccag attgacgccg ccgatcaggt cggcgctgtt
     2581 ttccttctgc gccagcaggc gagcctgggt gctcgacgcc cccttctcgg acaggacgaa
     2641 gaccgaggaa ccacgcgcat cgggcttgat ccaggcgtcg gcatggatcg acaggaagag
     2701 gtcggactgg atgcgtcgcg ccttctgcac gcgcacctgc agcggaacga agaaatcgcc
     2761 gtcgcgggtg agcacggcgc gcatgttcgg ttcagcgtcg atgcgcgcct tcaggcgacg
     2821 agcgatggcc agcgtgaccg ccttttcgtg ggtaccgcgc ttgccgacgg caccgggatc
     2881 ctcgccgcca tgcccgggat cgagcatgat cgtcaccagc cggtcgacca cggctttgcc
     2941 cgagcgcttc gccacctgcg gctctggcgc cttggccggc ttcgcttcct cgccgccgag
     3001 gtcctgcccc ggcttgagcg gctcgaccgc gtcgcgccgc ccttcgagca gcgccatcag
     3061 gggatcggcc ggggtttccg gatagatgtc gaggaccagg cggt
//
LOCUS       DAOSDX010000282         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11133_length_3103_cov_2.862861, whole genome shotgun sequence.
ACCESSION   DAOSDX010000282 DAOSDX010000000
VERSION     DAOSDX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11133_length_3103_cov_2.862861"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLN02_07205"
     CDS             <1..1025
                     /locus_tag="PLN02_07205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOI52152.1"
                     /translation="RDYLPAGVELIEEGVAEFDPEGRKIVTTGGKSIAYDYLIVATGC
                     KLDYAAIAGMDVEQIGNNGLGSIYHSPAKAEATWRAMSNFADNGGVGVFLRPGTEMKC
                     AGAPLKYTFITDDYLRRRGNRGKAELIYNANNKALFSVPIVHEKVRMLFEDRGVKVNY
                     ERILESIDLGKRLAVFKTALGPVELQYDFINVIPPMRAPDAVRNSPLRWQEGGWANDG
                     WIEVDKHKLRHKRFDNVFAVGDVAGVPKGKTAASVKWQVPVAVDHLVAQIEGKESSAH
                     YGGYTSCPLITRLGQAMLIEFDYKDNLTPSFPGVIAPLEELWISWVMKTMALKPTYIS
                     MLRGRA"
     gene            1045..1395
                     /locus_tag="PLN02_07210"
     CDS             1045..1395
                     /locus_tag="PLN02_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5368 domain-containing protein"
                     /protein_id="HOI52153.1"
                     /translation="MKELDPLVFLAVFQEMLGPLLWLMLLVIIAGTAAFIALLVKEKS
                     INSKRLVRAELAGLLGGVLALVLMAKVSSSGFTDAGGPADWFLIALVFGLGLIGTAVL
                     VYTAIGWRKPAANG"
     gene            complement(1401..2471)
                     /locus_tag="PLN02_07215"
     CDS             complement(1401..2471)
                     /locus_tag="PLN02_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOI52154.1"
                     /translation="MDLPTLLESFGEKTVITVTGLLIGILFGIFAQRSRFCLRSAVIE
                     FWRRESDGRLAIWLFAFAAAVVATQAAILAGWLDVAQARQLSARGSLSGALIGGLLFG
                     IGMILARGCSSRMLVLAANGNLRALLTGLIFAVTAQASLSGLLSPLRNWLSGLWTIEG
                     SARDLLVSAHLGNLGGLLFGLAWLAAAIVFGRRARLGFREWSCAIGVGLSVALAWLCT
                     FVIGSHAFEVMPVQSLSFTGPSANVLMLVLSPPGQPWDFDIGLVPGVVLGSFLAALWG
                     RELKLEGFKDGHSMRRYIAGALLMGFGGMLAGGCAVGAGVSGAAIFALTAWLALIGMW
                     GGAGLADTLIDGPARRNATPRP"
     gene            complement(2522..>3103)
                     /locus_tag="PLN02_07220"
     CDS             complement(2522..>3103)
                     /locus_tag="PLN02_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52155.1"
                     /translation="IEAARAGEAGRGFAVLADEVRSLAARTGEFNVDIRTALDDILKS
                     LRDVGERVNAASHIDLSVADRSRATLEDLGRELIELTEKAREHSTNITSITERMRDLT
                     LDGVMAMQFEDIVNQMITRISQRTENVGTYMHAFLSLHNDRDENDGVQRFRKRSQHLV
                     QLLVDSHVKTDALRSRTIQSGTGSSGVGDIEMF"
BASE COUNT          545 a          987 c         1060 g          511 t
ORIGIN      
        1 cccgcgatta cctgcccgcc ggcgtcgaat tgatcgagga gggcgtcgcc gagttcgatc
       61 ccgagggcag gaagatcgtc accaccggcg gcaagtccat cgcctacgat tacctgatcg
      121 tcgccaccgg ttgcaaactg gactacgcgg cgattgccgg catggatgtc gagcagatcg
      181 gcaacaacgg gctgggcagc atctatcaca gcccggccaa ggccgaagcc acctggcgcg
      241 ccatgtcgaa cttcgccgac aacggtggcg tcggcgtctt cctgcgcccg ggcaccgaga
      301 tgaagtgcgc cggggcgccg ctcaaataca ccttcatcac cgacgactat ctgcgccgcc
      361 gcggcaaccg cggcaaggcc gagctgatct acaacgccaa caacaaggcc ttgttctcgg
      421 tgcccatcgt ccatgagaag gtccgcatgc tgttcgaaga ccgcggcgtc aaggtcaatt
      481 acgagcgcat cctggaatcg atcgacctcg gcaagcgcct tgcggtgttc aagacggcgc
      541 tcggcccggt cgaattgcag tatgacttca tcaacgtgat tccaccgatg cgggcgccgg
      601 acgccgtgcg caacagcccg ttgcgctggc aggaaggcgg ctgggccaac gacggctgga
      661 tcgaggtcga caagcacaag ctgcggcaca agcgtttcga caatgtcttc gccgtcggcg
      721 atgtcgccgg cgtgcccaaa ggcaagaccg cggcgagcgt caagtggcag gtgccggtgg
      781 cggtcgacca cctggtcgcg cagatcgagg gcaaggaatc gtcggcgcat tacggcggtt
      841 acacctcgtg cccgctgatc acccgtctcg gccaggcgat gctgatcgag ttcgactaca
      901 aggacaacct gacgccgtct ttcccgggcg tcatcgcgcc gctcgaagag ttgtggatca
      961 gttgggtgat gaagacgatg gccctgaagc caacctatat cagcatgctg cgcggtcgcg
     1021 cctgattttc gaaggagtca aacgatgaaa gaactcgatc cgctggtttt cctcgctgtc
     1081 ttccaggaaa tgctggggcc gctgctgtgg ctgatgttgc tcgtcatcat tgccggcacg
     1141 gctgccttca ttgccctgct ggtcaaggag aaaagcatca attcgaagcg cctggtgcgt
     1201 gccgaactcg ccggcctgct cggtggcgtg ctggcgctgg tgctgatggc caaggtctcg
     1261 tcgtccggct tcaccgatgc cggcgggccg gccgactggt tcctgatcgc gctggttttc
     1321 ggtctcggct tgatcgggac ggcggtgctc gtctataccg cgatcggctg gcgcaagccg
     1381 gcggccaatg gctgactgaa tcacggccgg ggcgtcgcgt tgcggcgcgc cggcccgtcg
     1441 atcagtgtgt cagcaagacc ggcgccaccc cacatgccga tcagcgccag ccaggcggtg
     1501 agcgcgaaga tcgccgcacc ggaaaccccg gcaccgaccg cgcaaccacc ggcgagcatg
     1561 ccgccgaagc ccatcagcag ggcgccggcg atgtagcggc gcatgctgtg gccgtccttg
     1621 aagccttcca gtttcagttc gcggccccac aatgcggcaa ggaaggaacc cagcaccacg
     1681 ccgggcacca ggccgatgtc gaaatcccag ggctggccgg gcggcgacag caccagcatc
     1741 agcacgttgg ccgaggggcc ggtgaagctc aggctctgca ccggcatcac ctcgaaggcg
     1801 tggctgccga tgacgaaggt gcacagccag gcgagagcga cgctcagccc gacgccgatc
     1861 gcgcaactcc attcgcggaa gccgaggcgg gcgcggcgcc cgaagacgat cgccgccgcc
     1921 agccaggcga ggccgaaaag gaggccgcca agattgccga gatgggcgct gaccagcagg
     1981 tcgcgggcgc tgccttcgat cgtccatagc ccggaaagcc agttgcgcaa cggcgacagc
     2041 agcccgctca gcgaggcctg ggcggtgacc gcgaagatca gtccggtgag cagggcgcgc
     2101 aggttgccgt tggcggccag caccagcatg cgcgaggaac agccgcgggc gaggatcatg
     2161 ccgatgccga acagcaggcc gccgatcagg gcgccggaca ggctgccgcg cgccgatagc
     2221 tggcgggcct gcgcgacgtc gagccagccg gcgaggattg ccgcctgggt ggcgaccacg
     2281 gcggcggcga aggcgaacag ccagatcgcc aggcggccgt cgctttcccg gcgccagaac
     2341 tcgatgaccg ccgagcgcag gcagaaacgc gagcgctggg caaagatgcc gaacaggatg
     2401 ccgatcagca ggccggtgac ggtgatcacc gttttttccc cgaagctttc cagcagggtg
     2461 ggaaggtcca tgtttcgctc gcggctgcct gtgttcgggg tggggtttcc ggggcttggc
     2521 gtcagaacat ctcgatgtcg cctacgccgc tgcttcccgt tccgctctgg atggtgcgcg
     2581 aacgcagggc gtcggtcttg acgtgcgaat cgaccagcaa ctgcaccagg tgctggctgc
     2641 gtttgcggaa gcgctggacg ccgtcgtttt cgtcgcggtc gttgtgcagg ctgaggaagg
     2701 cgtgcatgta ggtgccgacg ttctcggtac gctggctgat gcgggtgatc atctggttga
     2761 cgatgtcctc gaactgcatc gccatcacgc cgtcgagcgt caggtcgcgc atgcgctcgg
     2821 tgatcgaggt gatgttggtc gagtgctcgc gcgccttctc ggtcagttcg atcagttccc
     2881 ggccgaggtc ctccagcgtc gcccgcgagc ggtcggcgac gctgaggtca atgtggctgg
     2941 cggcattgac ccgttcgccg acgtcgcgca gcgatttcag gatgtcgtcg agggcggtgc
     3001 ggatatcgac gttgaattcg ccggtacgcg ccgccagcga gcgcacctcg tcggcgagga
     3061 cggcgaagcc acggccggcc tcgccggcgc gggcggcttc gat
//
LOCUS       DAOSDX010000283         3101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11146_length_3101_cov_3.302364, whole genome shotgun sequence.
ACCESSION   DAOSDX010000283 DAOSDX010000000
VERSION     DAOSDX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11146_length_3101_cov_3.302364"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLN02_07225"
     CDS             complement(<1..515)
                     /locus_tag="PLN02_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOI52156.1"
                     /translation="MSNPNLEQLTAAQKANAEVMTALMRTAFNGLERLTALNMAATRE
                     FFNTTVASTQQLLAAKDANAVAKLGSELTQPSTEKLMDYSRSLYDLVTEMQKEVTSVM
                     EAQYSNFQQTAASAVEKAKTATPVGGDVFAATMQTMLDASTKAFGQMNSMAKQLSDIA
                     EANMQAATKAAA"
     gene            complement(597..1148)
                     /locus_tag="PLN02_07230"
     CDS             complement(597..1148)
                     /locus_tag="PLN02_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOI52157.1"
                     /translation="MFKTPEQFAAANKATVDSLLSLANTALASAERIAALNLNTARSL
                     MEDSVSGAKALMGAKDVQEALSIQASLAQPNVEKAVAYSRSVYEISAQTQEELSKMVE
                     AQFSDFQKTVSGLLDQAAKSAPAGSDVAVAAVKSAIAAANSAFDNMNKAAKQVAEIAE
                     ANVAAATNATVKAVSATAAPKKK"
     gene            complement(1339..1938)
                     /gene="lptA"
                     /locus_tag="PLN02_07235"
     CDS             complement(1339..1938)
                     /gene="lptA"
                     /locus_tag="PLN02_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOI52158.1"
                     /translation="MILRPALLAAIILGCPPAFAEKADRDKPMLLEANRISIDDAKKQ
                     QILEGDVVISKGTLMIKAARIIVSEDRYGFQKGIAFAPPGGKASFRQKRDGREDYVEG
                     EAERIEYDSNSEIAELFHRAHIRAGEDEVRGDYIWYDAVSEKFLASAGEASDPKGATP
                     RVRAVIQPRSKGSEPSRPSTRGDNLQLRSAGDIDPKDPR"
     gene            complement(1935..2519)
                     /gene="lptC"
                     /locus_tag="PLN02_07240"
     CDS             complement(1935..2519)
                     /gene="lptC"
                     /locus_tag="PLN02_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOI52159.1"
                     /translation="MKNWSGQLFPITLLALLAGLSFWLQAILTTDEVRPEMRTVAHEA
                     DAIVENFEIRRLDEQGKLQYRLSAPQLRHFPDNDSSLVTSPRLLAYRGDAPPLILTAG
                     RATVTARGETILLDDSVEIVRAATDKRPELNARTSTLTVEPETGKAHTDQAMTITLGA
                     SEISGVGARIDNKASTFELRSQVRGRYLSARAKP"
     gene            complement(2542..>3101)
                     /locus_tag="PLN02_07245"
     CDS             complement(2542..>3101)
                     /locus_tag="PLN02_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI52160.1"
                     /translation="LDAGVAQEACPLNLAPTASTTAALALGDALAVALLDARGFGPED
                     FARSHPGGSLGRRLLTHVRDVMRQRDRVPAVAPTTPISEAIVAMSHGGLGLVAVVDDT
                     DRPVGIFTDGDLRRAFEKRIDLQDASIVAAMTATPRTIGPERLAVEAVEMMERLRINA
                     LLVCDEDGKLQGALNMHDLFTAKVI"
BASE COUNT          450 a          876 c         1128 g          647 t
ORIGIN      
        1 gctgctgcct tggtggcggc ctgcatgttg gcttcggcga tgtcggacag ctgcttggcc
       61 atcgagttca tctggccgaa ggccttggtc gaggcgtcga gcatggtctg catggtcgcc
      121 gcgaacacgt cgccgccgac cggcgtcgcc gtcttggcct tttccaccgc gctggcagcg
      181 gtctgctgga agttgctgta ctgcgcttcc atcaccgagg tgacttcctt ctgcatttcg
      241 gtgaccagat cgtagaggct gcgcgaatag tccatcagct tctcggtgct cggctgggtc
      301 agttcgctgc ccagcttggc gacggcgttg gcgtccttgg cggcgagtag ttgctgggtg
      361 ctggcaaccg tcgtgttgaa gaactcgcgg gtcgccgcca tgttcagcgc ggtcaggcgc
      421 tcgaggccgt tgaaggcggt gcgcatgagg gcggtcatca cttcggcgtt ggctttctgt
      481 gctgcggtca gttgctcaag gttcgggttg ctcatcgtta tctccagcgt gcgttttata
      541 ggtggttggg atgaaacaaa gcctccgccc ggaggcagag gcctggatgc aacaacttac
      601 ttcttcttgg gggcggcggt ggcgctgacc gccttgaccg tggcgttggt ggcggcggca
      661 acgttggctt cggcaatctc ggcaacctgc ttggcggcct tgttcatgtt gtcgaaagcc
      721 gagttggcgg cggcgatggc gctcttgacg gcagcgacgg caacgtcgga gccggccggg
      781 gcggacttgg cggcttgatc gagcagaccg gagacggtct tctggaagtc ggagaactgg
      841 gcttcgacca tcttcgacag ttcttcctgg gtctgggccg agatttcata gacgctgcgc
      901 gagtaggcaa cggccttctc gacgttcggc tgggccagcg aggcctggat cgacagggct
      961 tcctggacat ccttggcgcc catcagcgcc ttcgcgccgg agacggaatc ttccatcagc
     1021 gagcgggcgg tgttcaggtt cagggcggcg atgcgttcgg cggaggcgag cgcggtgttg
     1081 gcgagggaca gcagggaatc gacggtggct ttgttggcag cggcgaattg ctcgggcgtc
     1141 ttgaacatgg gggtctccgg aaagaaaagt ctggcagaac aggggtggcc ttggcagtgc
     1201 cgtttcgctt tgcgttgctg caccgcacca tgtctttgat tatagacgag cgtgttgact
     1261 tgtcaacaac tattttgtgc actgcacaaa cgggataaat cctttcgaat cagccatgtg
     1321 attgatgcta atggtatttt atcgagggtc tttcgggtcg atgtcgccgg cgctgcgcag
     1381 ttgcagattg tcgccacggg tactcggacg gctcggttcg ctgcccttgc tgcgcggctg
     1441 gatcaccgcc cgcacgcgcg gtgtggcgcc cttcgggtcg ctcgcctcgc cagcgctggc
     1501 gaggaacttc tcgctgaccg cgtcgtacca gatgtagtcg ccgcggacct cgtcctcgcc
     1561 ggcgcggatg tgggcgcggt ggaaaagttc ggcgatctcg ctgttgctgt cgtattcgat
     1621 gcgctcggct tcgccttcga cataatcttc gcgcccgtcg cgtttctggc ggaagctcgc
     1681 cttgccgccc ggcggggcga aagcgatgcc cttctggaag ccatagcggt cctcgctgac
     1741 gatgatgcgc gccgccttga tcatcagcgt gcccttgctg atgacgacat cgccttcgag
     1801 gatctgctgc ttcttggcgt cgtcgatcga gatgcggttg gcttcgagca gcatcggctt
     1861 gtcgcggtcg gccttttcgg cgaaggcggg ggggcagcca agaatgatgg cggcgagcag
     1921 ggcggggcga aggatcatgg cttggctctg gcggaaaggt agcgaccgcg gacttgcgag
     1981 cgcagttcga aggtcgaggc cttgttgtcg atgcgggcgc cgacgccact gatttcggag
     2041 gcgcccaggg tgatggtcat ggcctggtcg gtatgggctt tgccggtttc cggttcgacg
     2101 gtcagcgtcg aggtccgggc attgagttcg gggcgtttgt cggtggcggc gcggacgatc
     2161 tccacgctat cgtcgagcag gatcgtttcg ccgcgggcgg tgaccgtcgc ccgaccggcg
     2221 gtcaggatga gcgggggcgc gtcgccgcga taggcgagca ggcgcggcga ggtgaccagc
     2281 gaactgtcgt tgtcggggaa atggcgcagt tgtggcgcac tcagacggta ttgcagtttg
     2341 ccttgctcgt cgaggcggcg gatttcgaaa ttttcgacga tggcatcggc ctcatgggcc
     2401 accgtgcgca tctccggacg cacttcgtcg gtggtgagta tcgcctgcag ccagaaactg
     2461 agccccgcca gcagcgccag cagggtgatc gggaagagtt ggccggacca gttcttcatg
     2521 gcaggagtgt gatggcgctc atcagatgac cttggcggtg aacaggtcgt gcatgttgag
     2581 cgcaccctgc agcttgccgt cctcgtcgca gacgagcagg gcattgatgc gcaggcgttc
     2641 catcatctcg accgcctcga cggcgaggcg ctcagggccg atggtgcgcg gggtggcagt
     2701 catcgcggcg acgatgctgg cgtcctggag gtcgatgcgt ttctcgaagg cgcgccgcag
     2761 gtcgccgtcg gtgaagatgc cgaccggccg gtcggtgtca tcgaccacgg caaccagacc
     2821 gaggccgccg tgggacatgg cgacgatggc ctcgctgatc ggtgttgtcg gcgctaccgc
     2881 cggcacccgg tcgcgctggc gcatgacgtc gcggacgtgg gtcagcaggc ggcggccgag
     2941 cgatccgccg gggtgcgagc gggcgaaatc ctccgggccg aagccgcggg cgtcgagcag
     3001 ggcgaccgcc agtgcgtcgc cgagggccag cgcggcggtg gtcgaggcgg ttggcgccag
     3061 gttgagcggg caggcttcct gggcgacccc ggcgtcgagg t
//
LOCUS       DAOSDX010000284         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11173_length_3096_cov_3.019730, whole genome shotgun sequence.
ACCESSION   DAOSDX010000284 DAOSDX010000000
VERSION     DAOSDX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11173_length_3096_cov_3.019730"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLN02_07250"
     CDS             complement(<1..214)
                     /locus_tag="PLN02_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52161.1"
                     /translation="MRLTLLVPELIWPEPGDTQTLAELSTPAFAWLDGHAAFSRGEPL
                     AVESALLEQFGIADAGSAAFRRLGETD"
     gene            complement(272..442)
                     /locus_tag="PLN02_07255"
     CDS             complement(272..442)
                     /locus_tag="PLN02_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52162.1"
                     /translation="MHFALLFLILAALAATLFPDWGWPAVLGSAGAAVVVLRLLLMLG
                     GGRGKSADDAAN"
     gene            543..1751
                     /locus_tag="PLN02_07260"
     CDS             543..1751
                     /locus_tag="PLN02_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI52163.1"
                     /translation="MTTELTPRNERLLLLVLAAIQFGHILDFMIMMPLGPILMRAFAI
                     NAGEFGFLVASYSFSAAAAGLLGATFVDRFERKRLLLVVVALFGLATLACALAPGYAT
                     LLVARGLAGTFGGLLGALVQTMIGDAIPFARRAQATGVVASAFSISTIAGVPLSLWLA
                     NHLGWHAPFVLVAAIAAVCIAVGRRCLPELHGHLGAPAHPLAAAGEVMKEGNHRRALL
                     FSALVIFSGFMVIPYITIQAVGNVGIAQSEIPLVYLVGGGATFFSARAIGRWADRRGK
                     VAVYRWLALAALLPLLLVTHAGHLPLWGWLVCSTSFFVLISGRMIPAMAIVTSAAQPR
                     LRGTFMSLNGTVQALAMGLATTLGGALIEVDAAGHLSGYPLVGYVAMAANLLAVAWVA
                     RIAMHTRPAG"
     gene            1821..2084
                     /locus_tag="PLN02_07265"
     CDS             1821..2084
                     /locus_tag="PLN02_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52164.1"
                     /translation="MSRSFLTGAAFGAALLGAALLLNPSADRHREKIKTVLAERSQLN
                     AAFGVGALVAFASNYHSLGIASYTTAGDQTLSFGMLGMVFVLD"
     gene            2164..2982
                     /locus_tag="PLN02_07270"
     CDS             2164..2982
                     /locus_tag="PLN02_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOI52165.1"
                     /translation="MRSLLIFLFCLVLAACGNKHEADSQAMAPAELAGSAAGAPAPAD
                     SPRRYMAYEHTIKLEVAEERVAAVHQQAEAACLAAAAEQCVILESRIGKGRGSYAQIT
                     LRAKAAGIRQLMSALSAQGRVASQSTTAEDLAGPLEDSAKKLAMLNDYRGKLEALRNK
                     ASGDIDAMITVNRELAQTQAQIEEISGARAHDLQRVNTEVLRLSITATGARSSWRPVR
                     DALDEFVDNLARGMSGAITAIAYLIPWSIAFLLVGGLARLLWRRFRKPAPKAAA"
BASE COUNT          447 a         1055 c         1045 g          549 t
ORIGIN      
        1 cgtcggtttc gccgaggcga cggaaagcgg cactgccggc gtcggcgatg ccgaattgtt
       61 cgagcagcgc ggattcgacg gccagcggtt cgccgcggct gaaggcggcg tgcccgtcga
      121 gccaggcgaa ggccggagtg ctcagctcgg cgagggtttg cgtgtcgccc ggttcgggcc
      181 agatcagttc ggggacgagc agggtgaggc gcacggcgaa actcggggca ggggaagccg
      241 cgagtttacc atcgcgcggc ggccggccgg ctcagttcgc ggcgtcgtcg gccgatttgc
      301 cgcggccgcc gccgagcatc agcaggaggc gcagaacgac gacggcggcg ccggcgctgc
      361 cgagcaccgc cggccagccc cagtcaggga acagggtagc ggcgagtgcc gccaggatca
      421 ggaagaggag ggcgaaatgc atggccgcat ggtaagtcgc cggcggtgcc ggtgacaatc
      481 ccgcactacc ggtccgccgc cgcgccgcgt atccttggcg tcccccgcaa tcctgcccgc
      541 caatgacgac cgaactgact ccgcgcaacg aacgcctgct cctgctggtc ctggccgcca
      601 tccagttcgg ccatatcctc gatttcatga tcatgatgcc gctcgggccg atcctgatgc
      661 gggctttcgc catcaacgcg ggcgaattcg gtttcctggt cgcatcctac agtttcagcg
      721 cggcggccgc cggcctgctc ggcgccacct tcgtcgaccg tttcgaacgc aaacgcctgc
      781 tgctcgtcgt cgtcgccctg ttcgggctgg cgacgctggc ctgcgcgctg gcgccgggtt
      841 acgccacgct gctcgtggcg cgcgggctgg ccgggacctt cggcggcctg ctcggggcgc
      901 tggtgcagac gatgatcggc gacgccatcc cgttcgcccg tcgggcccag gcgaccggcg
      961 tcgttgccag cgccttctcg atctcgacca tcgccggcgt gccgctgtcg ctgtggctgg
     1021 ccaaccacct cggctggcac gcgcccttcg tgctggtggc ggcgattgcc gccgtctgca
     1081 tcgccgtcgg ccggcgctgc ctgccggaac tgcacggcca tctcggcgcg ccggcgcatc
     1141 cgctggcggc ggccggcgag gtgatgaagg aaggcaacca tagacgcgcc ctgctgtttt
     1201 cggcgctggt catcttttcc gggttcatgg taattcccta catcacgatc caggcggtcg
     1261 gcaacgttgg tatcgcccag tcggagattc ccctcgttta tctggtcggc ggcggcgcca
     1321 ccttcttcag tgcccgcgcc atcgggcgct gggccgaccg ccgcggcaag gtggcggtct
     1381 atcgctggct ggccttggcg gcgctgttgc cgctgctgct cgttacccac gccggacact
     1441 tgccgctgtg gggctggctg gtctgttcga ccagtttctt cgtgctgatc tcggggcgca
     1501 tgattcccgc catggcgatc gtcacctcgg ctgcccagcc gcgcctgcgc ggcaccttca
     1561 tgtcgctcaa cggcaccgtg caggcattgg cgatgggctt ggcgacaacc ctcggcggcg
     1621 ccctgatcga ggtcgatgcg gcgggccacc tgagcggtta tccgctggtc ggctatgtcg
     1681 cgatggccgc caacctgctg gcggtcgcct gggtcgcccg catcgccatg catacgcggc
     1741 ctgccggctg aacgccgggt gtgttagcct gcacggccgc acttttcacc ccgaagtcat
     1801 atccttccag ggagttctcc atgagccgtt ccttcctgac cggcgccgcc ttcggcgctg
     1861 ccctgctggg cgcagccttg ttgctcaatc cgtcggccga tcgtcatcgc gagaagatca
     1921 agacggtgct cgccgagcgt agccagctca acgccgcctt tggcgtcggc gcgctggtcg
     1981 ctttcgcttc caactaccac agcctgggca tcgcgtccta caccacggcg ggcgaccaga
     2041 ccttgtcgtt cggcatgctc ggcatggtct tcgtgcttga ttaggcgcgg cgctgcctga
     2101 aagccgggca gcttttgttg gtatcccacg aagttccttg acgcattcat ccgggagcaa
     2161 gcgatgcgtt cgttgttgat tttcctcttc tgccttgtcc ttgccgcttg cggcaacaaa
     2221 cacgaagccg acagccaggc gatggcgccg gcggagctcg ccggcagcgc ggcaggcgca
     2281 ccggcaccgg ccgacagtcc gcgtcgctac atggcctacg agcacaccat caaacttgag
     2341 gtggctgagg aacgcgtcgc cgcagtgcac cagcaggccg aagccgcctg cctggccgcg
     2401 gcggccgagc aatgcgtcat cctcgaatcg cgcatcggca aggggcgggg cagttatgcg
     2461 cagatcaccc tgcgcgccaa ggcggccggc atccggcaac tgatgagcgc cctgagcgcc
     2521 cagggcaggg tcgccagcca gtcgacgacg gcggaggacc tggccggtcc gcttgaagat
     2581 tcggccaaga agctggccat gttgaacgac tatcgcggca agctcgaagc cctgcgcaac
     2641 aaggcgagcg gcgatatcga cgcgatgatc acggtgaacc gggaactggc ccagacgcag
     2701 gcacaaatcg aggaaatcag cggcgcccgc gcccacgacc tgcaacgcgt gaataccgag
     2761 gtgctgcgcc tgtcgattac ggcgactggt gcaaggtcgt cctggcggcc ggtgcgcgac
     2821 gcgctggacg agtttgtcga taacctggcg cgcggcatgt cgggcgcgat cacggcgatt
     2881 gcctacctca ttccgtggag catcgccttc ttgctggtcg gcgggctggc ccgcctgctc
     2941 tggcgccgtt tccgcaagcc ggcgccgaaa gcggcggctt gagcgccggc gcgttaaact
     3001 gccagccctt tttccccgga gtcgagcgat gaacgaggcc gtttactgga tactgcgctg
     3061 ccagcagcgc gtgctcaccg tgcccggcga ctggcc
//
LOCUS       DAOSDX010000285         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11177_length_3095_cov_3.449013, whole genome shotgun sequence.
ACCESSION   DAOSDX010000285 DAOSDX010000000
VERSION     DAOSDX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11177_length_3095_cov_3.449013"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PLN02_07275"
     CDS             complement(<1..1218)
                     /locus_tag="PLN02_07275"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOI52166.1"
                     /translation="MLSEQLVDLSRLQFAITALYHFLFVPLTLGMTWILVIMEASYVM
                     TGKTIYKDMTRFWGKLFGINFALGVTTGITMEFQFGTNWAYYSHYVGDVFGAPLAIEG
                     LMAFFLESTLIGLFFFGWDRLSRRQHLMVTIFMAIGTNLSALWILIANGWMQNPVGAE
                     FSYVTMRMEMVDFWAVLFNPDAQAKFVHTVSAGYTTGAIFVLAISSFYLLRGRDVEFA
                     KKSFRVAAAFGFASVCSVIVLGDEAGYSIGEAQQTKLAAMEAMWETEPAPAGFNLIAI
                     PNEKEMKNDFAIEIPWVMGIIGTRSLDKQIPGIKEIRETNRQRILSGQIAVTALEALR
                     QNPADAATRAQLDQHVKDLGFGLLLKKYVSDVRQATPEMIDKAVSDTIPSVAPMFWTF
                     RIMVAFGFLMLALF"
     gene            1525..2817
                     /locus_tag="PLN02_07280"
     CDS             1525..2817
                     /locus_tag="PLN02_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOI52167.1"
                     /translation="MLSELISFLETLPEPHILCDRENRIIAANDAYRNAWTDQREVVG
                     RKCYEVSHGYEVPCNEAGEFCPLQRSLHSGQRERVLHLHHTPDGETFENIELSPIRAE
                     SGEIVYYIEKLEPLPGARSQAHGQGLIGRSPPFLHMMEMVARVAPSEAAVLLQGESGT
                     GKELVAGAIHRMSKRAEQPFVAVDCSGLPETLFESELFGHERGAFTGASARKTGLVEA
                     AAGGTLFLDEVGDIPLSIQVKLLRLLETGTYRRVGSPELRKAEIRIVSATHRPLADMV
                     EAGTFRQDLYFRLNIFPIFLPPLRERGEDILLLAEALLARLANGRPLTLSRAARGWLA
                     GYRYPGNVRELRNILERACLLCDGEEIQVEHLPGLAQARFGAAGEAVAPRPAIIDDLE
                     RLLESHGGSRKMLAEQLGISERTLYRRLLDLRQRGRIR"
     gene            2924..>3095
                     /locus_tag="PLN02_07285"
     CDS             2924..>3095
                     /locus_tag="PLN02_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOI52168.1"
                     /translation="MSIINSKLKPFVVQAYQDGKFITVSDGDLKGKWSIFFFYPADFT
                     FVCPTELGDMADL"
BASE COUNT          603 a          902 c          982 g          608 t
ORIGIN      
        1 gaacagcgcc agcatcagga acccgaaggc gaccatgatg cggaaagtcc agaacatcgg
       61 ggcgaccgac gggatggtgt cggaaacggc cttgtcgatc atttccggcg ttgcctgacg
      121 cacgtcgctg acgtacttct tgagcagcag gccaaagccc agatccttga cgtgctggtc
      181 gagctgggct cgcgttgcag cgtcggccgg gttctggcgc agggcttcca gcgcggtgac
      241 ggcgatctgg ccggaaagga tgcgctggcg gttggtttcg cggatttcct tgatgccggg
      301 gatctgcttg tccagcgagc gcgtgccgat gatgcccatg acccagggaa tctcgatcgc
      361 gaagtcgttc ttcatttcct tctcgttggg aatggcgatc aggttgaagc cggccggtgc
      421 cggttcggtt tcccacatcg cttccattgc cgcgagtttg gtctgctgcg cttcgccgat
      481 gctgtagccg gcttcgtcgc cgagcacgat gaccgagcag acggaagcaa agccgaaggc
      541 ggcggcgacg cggaagctct tcttggcgaa ctcgacgtcg cggccgcgca gcaggtagaa
      601 ggacgagatg gcgaggacga agatggcacc ggtggtgtaa ccggccgata cggtatggac
      661 gaacttggcc tgggcatccg ggttgaacag cacggcccag aaatcgacca tttccatgcg
      721 catggtcacg tagctgaact cggcaccgac cggattctgc atccagccgt tggcgatcag
      781 gatccacagt gccgacaggt tggtgccgat cgccatgaag atggtgacca tcagatgctg
      841 gcggcgcgac aggcggtccc agccgaagaa gaacaggccg atcagcgtcg attcgaggaa
      901 gaaggccatc agcccttcga tcgccagcgg cgcaccgaag acgtcgccga cgtagtgcga
      961 gtaataggcc cagttggtgc cgaactggaa ctccatcgtg atgccggtgg tgaccccgag
     1021 cgcgaagttg atcccgaaca acttgcccca gaaacgggtc atgtccttgt agatggtttt
     1081 gccggtcatc acgtaactgg cttccatgat gacgagaatc caggtcatgc cgagggtcag
     1141 gggcacgaac aggaagtggt acagggcggt gatcgcgaac tgtaaccggg acaggtcgac
     1201 gagctgctca ctgagcatcg gtttctcctt gtgatgcaat gatcgaatga cggaccctgg
     1261 cccgtctctg ggatcagcct gcggacgatc gtttccgcga gagtgcggag gaaggactgc
     1321 agaagctaga acagcattta cttatcaaga cgcgtgccag ccggcagttc tcagttggct
     1381 cgagctaagt atttgaaatt aaatttttat tttaatgtct tgtaaaccgc cgaaggctgt
     1441 cgactgccat ttggcagtta aatggcagac agtaatggca gttgatggca gaattgctct
     1501 accccatggt gactggaagc ggcaatgctc agcgaactga tttctttcct ggaaaccctg
     1561 cccgaaccgc acatcctgtg cgaccgtgag aaccggatca tcgccgccaa cgatgcctat
     1621 cggaacgcct ggaccgacca gcgcgaggtg gtcgggcgca agtgttacga ggtttcccat
     1681 ggctacgaag tgccatgcaa cgaagccggc gagttctgcc cgctgcagcg cagtctgcat
     1741 tcgggccagc gcgagcgggt cctgcatctg catcacacgc cggacggcga gactttcgag
     1801 aatatcgagc tgtcaccgat tcgcgccgag agcggcgaaa tcgtctatta catcgagaag
     1861 ctcgaacccc tgccgggggc gcgcagccag gcgcatgggc agggcctgat cggacgttcg
     1921 ccgcctttcc tgcacatgat ggaaatggtc gcccgggtcg cgccgtcgga ggcggcggtg
     1981 ttgctgcaag gcgaatcggg gactggcaag gagctggttg ccggcgccat tcaccggatg
     2041 agcaagcggg cggagcagcc tttcgtcgcc gtcgactgtt ccggtttgcc ggaaaccctg
     2101 ttcgagagtg agttgttcgg gcacgagcgg ggcgctttta ccggcgcttc ggcacgcaag
     2161 accgggctgg tcgaggccgc tgccggcggc accttgttcc tcgacgaggt gggggacatt
     2221 ccgttgtcga tccaggtcaa gctgctgcgc ctgctggaga ccggcaccta ccggcgggtc
     2281 ggttcgccgg aattgcgcaa ggcggaaatc cgcatcgtct cagccaccca ccggccgctc
     2341 gccgacatgg tcgaggccgg caccttccgc caggacctct atttccggct caacatcttt
     2401 cccatcttcc tgccgccgct gcgcgagcgc ggcgaagaca tcctgctcct ggccgaagcc
     2461 ctgctcgcca ggctggccaa cgggcggcca ctgacgctgt cgcgggcggc ccggggctgg
     2521 ctggccggct atcgctaccc gggcaacgtg cgcgagttgc gcaatatact ggagagggct
     2581 tgcctgcttt gcgatggcga ggaaatccag gtcgaacatc tgcccgggct ggcccaggcc
     2641 aggttcggcg ccgccggcga agcagtagca ccgcgaccgg cgatcatcga cgatctggag
     2701 cgcctgcttg aaagccatgg cggcagccgc aagatgctgg ccgaacaatt gggtatctcg
     2761 gagcggaccc tgtatcgccg tctgctcgat ttgcggcagc gcggacgaat tcgatagtca
     2821 cgcctcattg atcggatttg tttaatcaat tggattgatt ggttcggctt ttttatgatt
     2881 cattccgtcg cagccaatca cccagcccac tcaggagaaa aacatgtcca tcatcaacag
     2941 caagctcaag cctttcgtcg tccaggccta ccaggacggc aagttcatca ccgtgtccga
     3001 tggcgatctg aagggcaagt ggtcgatctt cttcttctat ccggccgact tcaccttcgt
     3061 ctgcccgacc gaactcggcg acatggccga tctgt
//
LOCUS       DAOSDX010000286         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11201_length_3089_cov_4.226763, whole genome shotgun sequence.
ACCESSION   DAOSDX010000286 DAOSDX010000000
VERSION     DAOSDX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11201_length_3089_cov_4.226763"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLN02_07290"
     CDS             complement(<1..444)
                     /locus_tag="PLN02_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI52169.1"
                     /translation="MGKLSDILDIAHSRAEGLERPYQGELLPAEAYELLQHAPGARLV
                     DVRTRAEWDWVGRIPGAVEIEWNQYPGGTRNPHFLAELRRQVDPEALLIFICRSGVRS
                     IGAAAAASDAGYTNCYNVLEGFEGDKDASGHRNTVGGWRRAGLPWH"
     gene            494..1660
                     /locus_tag="PLN02_07295"
     CDS             494..1660
                     /locus_tag="PLN02_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOI52170.1"
                     /translation="MGKVDSGPPGSAADRAGEAQKATWISVLVNLALTVAQLIVGWLA
                     HSQSLVAHGLHSFSDLLSDFLVIWASRQSVVPADRAHPYGHGRVETAATLALGVSLVL
                     IGAGILWDSSMRLGEHGSQPAIAMQAFWIAVFTVVAKEGLYRYLIRVAEKLRSQLLTA
                     NALHTRADAASALVVVVGIGGALLGWGFLDLLAAALMGFMILRMGGKLAWEAIEELID
                     TGLDEARVTAIRNTLCATPGVLDLHALRTRRMAHQVLVDAHVQVDPRISVSEGHHIAE
                     SARAAVLREHPEVLDVLVHVDPDDDVEPDRSALGLPGRESLLPQLAPLLADLPEPDRI
                     VLHYVGGQVEVDLYFAQGSLAGCASLCEMESRLGDRLKAVPVVHALRLNCTAAP"
     gene            1848..>3089
                     /gene="glnA"
                     /locus_tag="PLN02_07300"
     CDS             1848..>3089
                     /gene="glnA"
                     /locus_tag="PLN02_07300"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOI52171.1"
                     /translation="MATPQDVINMIKENDAKFVDFRFTDTRGKEQHVTVPVSAFDEDK
                     FENGHAFDGSSIAGWKGIQASDMLLMPDPATAYIDPFFDETTVVLSCDVVDPADGRGY
                     DRDPRSIAKRAEAYLKSCGVGDTAYFGPEPEFFIFDGVEWSVDMSGCNLKIHSGEAAW
                     KSGEANDGNGSSGHRPGVKGGYFPVPPVDSLHDIRSAMVLTLESLGVPVEVHHHEVAN
                     AGQCEIGTVFSTLVKRADWTQILKYVVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQS
                     IWKDGKNLFAGDQYAGLSETALYYIGGIIKHAKALNAITNPGTNSYKRLVPHYEAPVK
                     LAYSAKNRSASIRIPFVASTKARRIETRFPDPIANPYLCFSALLMAGLDGIQNKIHPG
                     DPADKNLYDLPPEEDAKIPTVC"
BASE COUNT          524 a         1013 c          963 g          589 t
ORIGIN      
        1 atgccagggc aggccggcgc gtcgccagcc gccgacggtg ttgcgatggc cgctggcgtc
       61 cttgtcaccc tcgaagcctt cgagcacgtt gtagcagttg gtgtagccgg cgtcgctggc
      121 ggctgccgca gcgccgatcg aacggacgcc gctgcggcag atgaagatga gcagcgcttc
      181 cgggtcgacc tggcggcgca gttcggccag gaaatgcgga ttgcgggtgc cgcccgggta
      241 ctggttccac tcgatctcga cggcgccggg aatgcggccg acccagtccc attcggcgcg
      301 agtccgcacg tcaaccaggc gggcgcccgg ggcgtgctgc aggagctcgt aggcttcggc
      361 cggcaacagc tcaccctggt agggacgttc caggccttcg gcccgggaat gtgcgatgtc
      421 gagaatgtcg ctgagtttgc ccatggtgga tcgctgctag aatcttggcc ctacagtata
      481 taggacagac ccgatgggaa aagtggattc cgggccgccg ggcagtgctg cggaccgtgc
      541 cggtgaggca cagaaggcga cctggatcag cgtgctggtc aacctcgcgc tgaccgtcgc
      601 ccaactgatc gtcggttggc tggcgcactc gcaatcgctg gtggcgcacg gcctgcactc
      661 cttttccgat ctgttgtccg attttctcgt catctgggcc agccggcaga gcgtggtgcc
      721 cgccgaccgc gctcacccct acggccatgg ccgcgtcgag acggcggcga cgctggcgct
      781 cggcgtctct ctggtgctga tcggcgccgg cattctgtgg gattcctcga tgcgcctcgg
      841 cgaacatggc agtcaaccgg cgatcgccat gcaagccttc tggattgccg ttttcaccgt
      901 tgtcgccaag gaaggcctct accgttacct gatcagggtt gccgagaaac tgcgttccca
      961 actgctgacc gcgaacgcct tgcatacccg ggccgacgcg gcgtcggcgc tggtggtcgt
     1021 ggtcggcatc ggcggcgcgc tgctcggttg gggcttcctc gacctgctgg cggcggcgtt
     1081 gatgggcttc atgatcctgc gcatgggcgg caagctggcc tgggaggcga tcgaggagct
     1141 gatcgacacc gggctcgacg aagcccgcgt cacggcgatc cgcaacacct tgtgcgcgac
     1201 gcccggtgtg ctcgacctgc atgccctgcg cacgcggcgc atggcgcacc aggtgctggt
     1261 tgatgcgcat gttcaggtcg atccgcggat cagcgtctcg gaagggcacc atatcgctga
     1321 atcggcgcgg gcggccgtgc tcagggagca cccggaagta cttgatgtgc tggtgcacgt
     1381 cgatcccgac gatgatgtcg agcccgatcg ttcggcgctc ggcctgcccg ggcgcgaatc
     1441 gctgttgccc caactggcgc cgctgctcgc cgatttgccg gagccggatc ggattgtcct
     1501 gcactatgtc ggaggtcagg tcgaggtcga tctctatttt gcccagggtt ctctggccgg
     1561 ctgtgcttcg ctgtgcgaga tggagtcgcg tctgggcgac cgcttgaagg cggtgccggt
     1621 ggtgcacgcg ttgcgcctga attgcacggc ggcaccatag tggtgctgtc gatctgtgta
     1681 tgcacaaaaa tggtgcgcat atttgcctat attccctgcc agtcccttgt ggcacaatga
     1741 aaaacttcgc tcgggaccct tggcatgctt tctgctatct ctcaggcgct ggattttgaa
     1801 tggcgccggc ccggccggca tcctgttttc ttaggagact aacgcgtatg gcaaccccgc
     1861 aagacgtgat caacatgatc aaggaaaacg acgccaagtt cgtcgatttc cgcttcaccg
     1921 acacccgtgg caaggaacag cacgttaccg tgccggtctc cgcttttgac gaagacaagt
     1981 tcgaaaacgg ccatgccttc gacggctcct cgatcgccgg ctggaagggt atccaggcct
     2041 ccgacatgct gctgatgccg gatccggcca ccgcctacat cgatcccttc ttcgacgaaa
     2101 ccaccgtcgt cctctcctgc gacgtcgtcg atccggccga cggccgcggc tacgaccgcg
     2161 atccgcgctc gatcgccaag cgcgccgaag cctacctgaa gtcctgcggc gtcggtgaca
     2221 ccgcctactt cggtccggaa cccgaattct tcattttcga cggcgtcgaa tggtcggtcg
     2281 acatgtccgg ctgcaacctg aagatccatt ccggcgaagc cgcctggaag tccggcgaag
     2341 ccaacgacgg caatggctcc tccggccacc gtccgggcgt caagggcggc tacttcccgg
     2401 ttcccccggt cgacagcctg cacgacatcc gttcggccat ggttctcacc ctcgaatcgc
     2461 tgggcgtgcc ggtcgaagta caccaccacg aagtggccaa cgccggccag tgtgaaatcg
     2521 gtaccgtgtt ctcgaccctg gtcaagcgtg ccgactggac ccagatcctg aagtacgtgg
     2581 tgcacaacgt tgcccaccag tacggcaaga ccgccacttt catgccgaag ccgatcgtcg
     2641 gtgacaacgg ctcgggcatg cacgtgcacc agtcgatctg gaaggacggc aagaacctgt
     2701 tcgccggtga ccagtacgct ggcctctcgg aaaccgccct ctactacatc ggcggcatca
     2761 tcaagcacgc caaggcgctg aacgcgatca ccaacccggg taccaactcc tacaagcgtc
     2821 tggtcccgca ctacgaagcg ccggtcaagc tggcctactc ggccaagaac cgttcggctt
     2881 cgatccgcat ccccttcgtc gcttcgacca aggcccgtcg tatcgaaacc cgcttcccgg
     2941 atccgatcgc caacccctac ctgtgcttct cggcgctgct gatggccggt ctggacggca
     3001 tccagaacaa gatccacccg ggcgacccgg ctgacaagaa cctctacgac ctgccgccgg
     3061 aagaggatgc caagatcccg accgtctgc
//
LOCUS       DAOSDX010000287         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11207_length_3088_cov_3.117376, whole genome shotgun sequence.
ACCESSION   DAOSDX010000287 DAOSDX010000000
VERSION     DAOSDX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11207_length_3088_cov_3.117376"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLN02_07305"
     CDS             <1..490
                     /locus_tag="PLN02_07305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI52172.1"
                     /translation="VRFVHAGAEAIPAPDASFDIVLMLKSLHHVPLPLMDSALSEVAR
                     VLKPGGLAWISEPVYAGDMNALLSLFHDEKIVREAAFAAVSRAVEQGPLTLDRQIFFQ
                     TRSKFDDFAHFEQRMIRVTHLDHVLSPELYAAVKARFESFMTPEGVTFRPPQRVDLLV
                     KR"
     gene            complement(550..625)
                     /locus_tag="PLN02_07310"
     tRNA            complement(550..625)
                     /locus_tag="PLN02_07310"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(590..592),aa:His,seq:gtg)
     gene            791..2098
                     /gene="tig"
                     /locus_tag="PLN02_07315"
     CDS             791..2098
                     /gene="tig"
                     /locus_tag="PLN02_07315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOI52173.1"
                     /translation="MEATTAQANELERRIDLSIAIADVEKEMEPRLKRMGRNMKVPGF
                     RPGKVPFAMVKQQYGDQARHEVLSEQLDRAFGTAVSEQKLLVAGYPRLEPKSTESTTH
                     IEFTAVFEVYPEFVPGDLSAAEIERPLLEVGAAEIEKTLDILRKQRVRYDNAERGAEK
                     EDRVVIDFTGKKDGVAFAGGSATDYPFVLGQGMMLPDFEKAVEGSKAGETKTFDLTFP
                     EDYHAKDLAGQTVQFEITVKQVQAPRLPEVDADFAKQMGVADGDVEKMRAEVEANLKR
                     EVKRRIEARLKDQVMEALLKANPIAIPNALVDMEIQRLMQAARQDMEQRGMKAKDMPL
                     QPEWFAENAKRRVTLGLILAEVVKTENLQAKPEQVRAMVEDMAASYEQPEEVIRWYYA
                     QPQRLSDVEGVAIENNVVEWVLGKAKVTDKAVAFDELMSQQQQ"
     gene            2154..2783
                     /gene="clpP"
                     /locus_tag="PLN02_07320"
     CDS             2154..2783
                     /gene="clpP"
                     /locus_tag="PLN02_07320"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOI52174.1"
                     /translation="MIIDNASNWDPQALGLVPMVVEQSGRGERAYDIYSRLLKERVIF
                     LVGPVNDASANLVVAQLLFLEAENPDKDIYFYINSPGGSVTAGMSIYDTMQFIKPDVS
                     TLCIGQAASMGAFLLCAGTKGKRFALPNSRVMIHQPLGGFQGQASDIAIHAREILSIK
                     DKLNRIMAEHTGQPLERIEKDTDRDNFLSSAEAMEYGLIDKVLTRRDAV"
     gene            2798..>3088
                     /locus_tag="PLN02_07325"
     CDS             2798..>3088
                     /locus_tag="PLN02_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOI52175.1"
                     /translation="MSKDGQEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIALCNDI
                     VRDELPEESAKAGRSDLPTPREISAILDQYVIGQEVAKRILAVAVYNHYKRLR"
BASE COUNT          667 a          979 c          913 g          529 t
ORIGIN      
        1 ggtccgtttc gtgcacgccg gcgccgaggc gatccccgcc ccggacgcca gcttcgacat
       61 cgtgctgatg ctcaagtcgc tgcaccacgt cccgctgccg ttgatggaca gcgcactttc
      121 cgaagtggcg cgcgtcctca aaccaggcgg cctggcgtgg atttccgaac cggtctacgc
      181 cggcgacatg aacgctctgc tcagcttgtt ccatgacgaa aaaatcgtcc gcgaagccgc
      241 tttcgcggcc gtctcccgcg ctgtcgagca aggcccgctg acgctcgacc gccagatatt
      301 cttccagacg cgcagcaagt tcgacgactt cgcgcatttc gaacagcgca tgatccgcgt
      361 cacccacctc gaccacgtgc tctcgcccga actctacgcg gcggtcaaag cccggttcga
      421 atccttcatg accccggaag gcgtcacctt ccgcccgccg caacgcgtcg acctgctggt
      481 caagcgctga atccggacgc gccaacggaa aaagcccgtg catcttgcga tgtacgggct
      541 tttgaattct ggggcgactg atggggctcg aacccacgac aaccggaatc acaatccggg
      601 actctaccaa ctgagctaca gccgccgctg caggaaggcg cgcattgtaa ggcctgtcgc
      661 cgtggctgtc tacaggcatt tgcaaatctt gcgcagcggc gattcgacgt acttggcgca
      721 atgcttgggc ggctcgccgt gatatgatta ccagctttat tttttggcgc ctcaagcaca
      781 aggaaaattc atggaagcta ctaccgcaca agccaacgaa ctcgaacgtc gcatcgacct
      841 ctccatcgcc atcgccgatg tcgagaagga aatggagccg cgtctgaagc gcatgggccg
      901 caacatgaag gtgccgggct tccgcccggg caaggtgccg ttcgccatgg tcaagcagca
      961 gtacggcgac caggctcgtc atgaagtgct gtccgagcaa ctggaccgcg ccttcggcac
     1021 cgcggtgagc gagcagaagc tgctcgtcgc cggctatccg cgcctcgaac cgaagagcac
     1081 cgaaagcacg acgcacattg aattcaccgc cgtcttcgag gtctatcccg aattcgtccc
     1141 gggcgacctg tcggccgctg aaatcgaacg tccgctgctc gaagtcggcg ctgccgaaat
     1201 cgaaaagacc ctcgacatcc tgcgcaaaca gcgcgtccgc tacgacaacg ccgagcgcgg
     1261 cgccgagaag gaagaccgcg tcgtcatcga cttcaccggc aagaaggacg gcgtcgcctt
     1321 cgccggcggc tcggccaccg attacccctt cgtcctcggc cagggcatga tgctgcccga
     1381 cttcgagaag gccgttgaag gcagcaaggc tggcgaaacc aagactttcg acctgacctt
     1441 ccccgaggat taccacgcca aggatctggc tggccagacc gtgcagttcg aaatcaccgt
     1501 caagcaggtc caggcaccgc gcctgccgga agtcgatgcc gatttcgcca agcagatggg
     1561 cgttgccgac ggcgatgtcg agaagatgcg cgccgaagtc gaagccaacc tgaagcgcga
     1621 agtgaagcgc cgcatcgagg cccgcctcaa ggatcaggtc atggaagccc tgctcaaggc
     1681 caacccgatc gccatcccga acgcgctggt agatatggaa atccagcgcc tcatgcaggc
     1741 cgcccgccag gacatggaac agcgcggcat gaaggcgaag gacatgccgc tgcagccgga
     1801 atggttcgcc gagaacgcca agcgtcgcgt cacgcttggc ctgatccttg ctgaagtggt
     1861 gaagacggag aatctccagg ccaagccgga acaggttcgc gccatggtcg aagacatggc
     1921 cgccagctac gaacagccgg aagaagtgat ccgttggtac tatgctcaac cgcagcgcct
     1981 gagcgatgtc gagggcgttg ccatcgaaaa caacgtggtc gaatgggtcc tcggcaaggc
     2041 caaggtcacc gacaaggccg tggctttcga tgaactgatg agccagcaac agcagtaagc
     2101 cgtattcgtc agaaaaccga cagacgtatc aaacgcgaca gacaagggcc aacatgatta
     2161 tcgacaacgc aagcaactgg gatccgcagg ccttgggcct ggtcccgatg gtggtggagc
     2221 agagcggacg cggcgaacgc gcgtacgaca tctattcgcg cctgctgaag gagcgggtga
     2281 ttttccttgt tggcccggtc aacgacgcca gcgccaacct ggtggtcgcc cagctcctgt
     2341 tcctggaagc cgagaacccg gacaaggaca tctatttcta catcaactcg ccgggtggct
     2401 cggtgactgc cggcatgtcg atctacgaca ccatgcagtt catcaagcct gacgtgtcga
     2461 cgctgtgcat cggccaggcg gcttcgatgg gtgccttcct gttgtgcgcc ggcaccaagg
     2521 gcaagcgttt cgcgctgccc aactcgcgcg tgatgatcca ccagccgctc ggcggcttcc
     2581 agggtcaggc ctcggacatt gcgatccacg cccgggaaat cctgtcgatc aaggacaagc
     2641 tgaaccgcat catggccgag cataccggcc agccgctgga acggatcgaa aaggataccg
     2701 accgcgacaa tttcctttcc tcggccgagg caatggaata tggtttgatt gacaaggtgc
     2761 tgacccgccg cgacgcggtt tgatcaagga gagagggatg tcgaaggacg gccaggaaaa
     2821 actgctctat tgctcgtttt gcggcaagag ccagcatgaa gtcaagaaac tgatcgccgg
     2881 cccgtcggtg ttcatctgcg acgagtgcat cgccctgtgc aatgatatcg tccgcgacga
     2941 actgccggag gaatcggcca aggccggtcg ttccgacctg ccgacgccgc gcgagatcag
     3001 cgccatcctc gatcagtacg tgatcggcca ggaagtcgcc aagcgcatcc tggcggtcgc
     3061 cgtctataac cactacaagc gcctgcgc
//
LOCUS       DAOSDX010000288         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11260_length_3075_cov_3.217219, whole genome shotgun sequence.
ACCESSION   DAOSDX010000288 DAOSDX010000000
VERSION     DAOSDX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11260_length_3075_cov_3.217219"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLN02_07330"
     CDS             <1..261
                     /locus_tag="PLN02_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOI52176.1"
                     /translation="RDDPLHRVDAGDSHGGGLTAPMPGKVVALLAQPGQKVDKGTPLL
                     ILEAMKMEHTITAPAAGTLKAFCYAAGEQVSDGAALVEFEGD"
     gene            372..746
                     /locus_tag="PLN02_07335"
     CDS             372..746
                     /locus_tag="PLN02_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52177.1"
                     /translation="MDDRLMQFGGQFAVPPSSFQRYHVYSFHHLDDLAFMKLCSEAFS
                     SFPDAQNYLDSIRSLFRGHGWEGDGRIQLFWLPPFVVNSHDTDGIYCFHVKQNNNGTS
                     WIASPVPLRFKGLNYLTEEIYP"
     gene            743..1648
                     /locus_tag="PLN02_07340"
     CDS             743..1648
                     /locus_tag="PLN02_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOI52178.1"
                     /translation="MTLPARVKIVEVGPRDGLQNEKQVIPTETKIELIERLVEAGLSV
                     IEATSFVSPKWVPQMGDNAAVLAGIRRRPGVTYTALTPNLQGFDGAVTAQADEVAIFA
                     AASEAFSRKNINCSIAESLKRFEPVVAAASALEIPVRGYVSCVVGCPYEGAVAPEKTA
                     EVAKVLYDMGCYEVSLGDTIGVGNPASIVRMIEACARVVPIDRLAGHYHDTYGMAIAN
                     IYASLQAGMAVFDASVAGLGGCPYAKGASGNVATEDVVYLLDGLGIQTGIDLAKLAAV
                     GEWIAATINRPNGARAGRALCAKSD"
     gene            1645..2634
                     /locus_tag="PLN02_07345"
     CDS             1645..2634
                     /locus_tag="PLN02_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52179.1"
                     /translation="MTFLSALADLLKPAPQPDPEVTAALDRVIGMVDPLVRAAPRLDK
                     HLLAAVEHALGYCHGLVSALPGPIEINRRSFANDMLVHALFATPTDIDEMLGRSQAVR
                     DFLQRPECWASDCFCAMLAARRLQKRQFGMAQQGNVIRSDVPQEVVYFSDQTLIEPSC
                     SLETTRQRLQARALESLLRSFNAHVDQLRRERQGLRADLSVEHAQLLALQQQHHADED
                     ERHTRHLVELDLRLRENSEALMPEHLVEVLAEFLRHPESALALTPVSIRIDRMGIVHE
                     PSEPGDDAQTIEFPELSSRDRRQHLATLVRITREEAQAAVAKSIDRQKRYVLL"
     gene            2634..2822
                     /locus_tag="PLN02_07350"
     CDS             2634..2822
                     /locus_tag="PLN02_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HOI52180.1"
                     /translation="MPALPASPCTNVCRIDPVSGFCVGCRRSLDEIANWSRLDAPARL
                     AVLAALRDRQAASPRPTE"
     gene            2825..2992
                     /locus_tag="PLN02_07355"
     CDS             2825..2992
                     /locus_tag="PLN02_07355"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HOI52181.1"
                     /translation="MSEPSEDLYPCVGICTTDPDSGYCLGCGRPPAEVPESAAPPQTP
                     APTADETPRPA"
BASE COUNT          587 a         1037 c          904 g          547 t
ORIGIN      
        1 cgcgacgacc cgctgcaccg cgtcgatgcc ggcgacagcc acggcggcgg cctgaccgcg
       61 ccgatgcccg gcaaggtcgt cgccctgctc gcccaacccg gccagaaggt ggacaagggc
      121 acgccgctct tgatcctcga agcgatgaag atggagcaca ccatcaccgc ccccgccgcc
      181 ggcaccctca aggccttctg ctacgcggcc ggcgaacagg taagcgacgg cgcggcgctt
      241 gtggagtttg aaggagacta atctcagaaa aatagccgaa cggaataaat aacttcgcat
      301 tttgtccaaa ttgcatcctt ccctacactg aaacctctgt ttcgccttac ccaatttcac
      361 aggagattca gatggacgac agattgatgc aatttggcgg gcaatttgca gttccaccat
      421 caagctttca gcgctatcac gtctattcat tccaccattt ggacgatcta gcttttatga
      481 aactttgcag cgaagctttt tccagttttc ctgatgcgca aaactatctc gactcaatta
      541 ggtcgttatt tcggggacat ggctgggaag gagatggaag aatccagcta ttttggctgc
      601 cgccgtttgt cgttaattcg cacgacactg acggaattta ttgttttcac gttaagcaaa
      661 acaacaacgg cacctcttgg atcgcatccc ctgtaccttt gcgcttcaag ggtttgaatt
      721 acctaactga agaaatctat ccatgactct ccctgctcgc gtcaaaatcg tcgaagtcgg
      781 cccgcgcgac ggattgcaga acgaaaaaca agtcatcccg accgaaacca agatcgaatt
      841 gatcgaacgc ctggtcgagg ccgggttgtc ggtgatcgag gcgacttcct tcgtctcgcc
      901 gaagtgggtg ccgcagatgg gcgacaacgc cgccgtgctg gccggcatca ggcgccggcc
      961 gggcgtcacc tacaccgcgc tgacgcccaa cctgcagggc ttcgacggtg ccgtcacggc
     1021 gcaggccgac gaggtggcga tcttcgctgc cgcttccgag gctttttcgc gcaagaacat
     1081 caactgttcc attgccgaga gcctgaaacg cttcgagcca gtggttgccg ccgcttcggc
     1141 gctggaaatc cctgtgcgtg gctatgtttc ctgcgtcgtc ggttgccctt atgaaggggc
     1201 ggtggcgccg gagaaaaccg ccgaggtggc gaaagtcctt tacgacatgg gctgctacga
     1261 ggtgtcgctc ggcgacacca tcggcgtcgg caatccggct tcgatcgtgc ggatgatcga
     1321 agcctgcgcc cgcgtcgtgc cgatcgacag gctagccggc cattatcacg atacctacgg
     1381 catggcgatc gccaacatct acgcttcact gcaggccggc atggcggtct tcgacgcctc
     1441 ggtcgccggg ctgggcggct gtccctacgc gaaaggcgcc tccggcaacg tggcaaccga
     1501 ggacgtggtc tatctgctcg acggattggg catacaaacc ggcatcgatc tggctaaact
     1561 ggcggcagtc ggcgaatgga tcgccgccac catcaaccgc ccgaacggcg ccagggccgg
     1621 ccgggcgctc tgcgcgaagt ccgattgact ttcctttccg cccttgccga tctgctgaag
     1681 cccgcaccac agcccgaccc cgaggtgacg gcggcgcttg atcgcgtgat cggcatggtc
     1741 gatccgctgg tccgcgccgc gccacgcctc gacaagcacc tgctggcggc cgtcgaacat
     1801 gcactgggtt attgccacgg cctggtcagc gccttgcccg ggccgatcga gatcaaccgg
     1861 cgcagcttcg ccaacgacat gctggtgcat gcgctgttcg cgacgccgac cgacatcgac
     1921 gaaatgctcg gccgcagcca ggcggtgcgc gatttcctgc aacgccccga gtgctgggcc
     1981 agcgactgct tctgcgcaat gctggcggcg cgccggctgc agaagcgaca atttggcatg
     2041 gcgcagcagg gcaacgtgat ccgcagcgac gtgccgcagg aagtcgtcta tttttccgac
     2101 cagacgctga tcgaacccag ttgcagcctg gagacgacgc gccagcggct gcaggcgcgc
     2161 gcactcgaga gcctgctgcg cagtttcaac gcccacgtcg accagttgcg ccgcgaacgc
     2221 caggggctgc gcgccgatct ctcggtcgaa cacgcccagt tgctcgccct gcagcaacag
     2281 catcacgccg acgaggacga gcgccacacc cgccatctgg tcgaactcga cctgcgcctg
     2341 cgcgaaaaca gtgaggccct gatgcccgag catctggtcg aggtgctggc ggaattcctg
     2401 cgccatccgg aaagcgcgct ggcgctgacg ccggtcagca tccgcatcga tcgcatgggc
     2461 atcgtgcacg agccgtccga acccggcgac gacgcacaaa ccatcgaatt cccggaactt
     2521 tccagccgcg accggcgtca gcatctggcg acgctggtcc gcatcacgcg cgaggaagcg
     2581 caggccgccg tcgccaaatc catcgaccgc cagaaacgtt acgtcctcct ctgatgccag
     2641 ccctgcccgc ctcgccctgt accaatgtct gccgcatcga cccggtcagc ggtttctgcg
     2701 tcggttgccg acgcagcctc gacgaaatcg ccaactggtc gcgcctcgat gcgccggcac
     2761 gcctggccgt gctcgccgcg ctgcgcgacc ggcaagcggc atctccccgc cccactgaat
     2821 gagcatgagc gagcccagcg aagacctcta cccctgcgtc ggcatctgca ccaccgaccc
     2881 cgacagcggc tactgcctcg gctgcgggcg gccgccggca gaggtgccgg aatccgccgc
     2941 cccaccccag acgccagcgc cgaccgcaga cgagactccg cgcccggcat gacggcgaag
     3001 ctgccagaca gcctcctggt catcgagcgc ggctggctgt cggccaacaa catcctcgct
     3061 ttcgacggcg agcgg
//
LOCUS       DAOSDX010000289         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11348_length_3054_cov_3.111370, whole genome shotgun sequence.
ACCESSION   DAOSDX010000289 DAOSDX010000000
VERSION     DAOSDX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11348_length_3054_cov_3.111370"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLN02_07360"
     CDS             complement(<1..186)
                     /locus_tag="PLN02_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52182.1"
                     /translation="MNTHSTEISVAPRHDALSGLLATLAGACRQLAIGETQCLRLQLI
                     AEELFINTVDHGRPDPER"
     gene            207..665
                     /locus_tag="PLN02_07365"
     CDS             207..665
                     /locus_tag="PLN02_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI52183.1"
                     /translation="MMIRAHRALAFMIAFLAVAAAQAAEVAGMVKISRGEAAIERAGE
                     RLPATVGTPVLATDRLRTGGDGALGVTLRDNTLLSAGPNSLIVIEKFAFNSSTLEGGM
                     SVGIRKGTLAVASGKIAKRTPESVDFHTPTSVLGVRGTEFLVEVIGGSDE"
     gene            658..1239
                     /locus_tag="PLN02_07370"
     CDS             658..1239
                     /locus_tag="PLN02_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI52184.1"
                     /translation="MSKTLPLFVLCLLLGACASERVVLLPSTDGSPGKVIVRDAAGER
                     VLDQPYAATARRLGINRPTVTEAAQVEVRYGETLRALPPRPTSYTVYFETGTDTMTPA
                     SAQAFVGVKREIAERPAVEVMVIGHTDRVGSPAENDPLSRQRAELVRDQLVAEGVPAE
                     KLEVAGRGEREPLVPTADDVDEPRNRRVEISIR"
     gene            complement(1226..>3054)
                     /locus_tag="PLN02_07375"
     CDS             complement(1226..>3054)
                     /locus_tag="PLN02_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOI52185.1"
                     /translation="AVGFDFIFAEADQHAPALLARRLPGLDPALLSHLPDPDQQFAEV
                     IGRAPTVLATVGLPLALAGARLPRHPLPQIAIEPGADRLLPRFASALASRPELQAAAG
                     GEGFINATPDARNGGHDRGILRRVPTLALIDEQPYLSLPLEMVRQALGGGAVAIEAGG
                     KGMAALRLGDYRLPTQANGELLLHFGRASSHYYLSAADILAGKHPPETFAGRLVIVGF
                     NSIGLQDRVITPLGDSLPGADVHVQVIESLLAGDALRRPDWMPMLELAALLAGGLLLI
                     GSVPALRPRYAVLGFAAVGGSLLLAGHLAYFSGRWLVDGNTPALLLGPVFMALLGNTL
                     IRADERHQAAERQLQASREQAARIAGELDSARRIQMGLLPDPERLFADETRFALAAIL
                     EPAQAVGGDYYDCFMLDTRRLCIAIGDVSGKGVPASLFMAISKTLGNTLTRRHDDLGA
                     AIRDLEHELDRNNTALLFVTAFIAILDADDGRLDYVCAGHDAPLLKRNGELQPLPMAE
                     ISGPPLCALGDYPYASGSIRLQAGDLLCLFTDGVSEATRDGQLFGHAGLEACLARTPE
                     TEPGTVCRQIRDSVRQFEAGEPAADDLTLLVLRWNGPRLSGC"
BASE COUNT          477 a          952 c         1157 g          468 t
ORIGIN      
        1 gcgttcgggg tccgggcggc catgatcgac ggtgttgatg aacagctctt cggcaatgag
       61 ttgcaggcgc aggcattgcg tttcgccgat cgccagctgc cggcaggctc cggccagcgt
      121 ggcgagcagc ccgctcagcg catcatggcg cggggcgacg gaaatctccg tagaatgcgt
      181 gttcatgact caggaaggag cttgggatga tgattagagc acatcgggcg ctggctttca
      241 tgatcgcttt tctcgccgtt gccgcggcgc aggcggcgga agtggcaggc atggtcaaga
      301 tcagccgcgg cgaggcggcg atcgaacggg ccggcgagcg tctgccggcg acggtgggga
      361 cgccggtact ggcgaccgat cgcctgcgca ccggtggcga cggcgcgctc ggtgtcacct
      421 tgcgcgacaa caccttgctt tcggcggggc cgaattcact gatcgtcatc gagaagttcg
      481 ccttcaacag ttcgacgctg gagggcggga tgtcggtcgg catccgcaag gggacgctgg
      541 cggtcgcgtc cggcaagatc gccaagcgga cgccggaatc ggtcgatttc cacacgccga
      601 cctcggtgct cggcgtgcgc ggcaccgaat ttctggtcga agtgatcggg ggcagcgatg
      661 agtaagacgc tgccactctt cgttctttgc ctgctgctcg gcgcctgcgc cagcgagcgg
      721 gtggtcttgc tgccgtcgac ggacggcagc ccgggcaagg tgatcgtccg cgatgccgct
      781 ggcgaacgcg ttctcgatca gccttatgcg gcgactgccc gccgcctggg tatcaatcgg
      841 ccgaccgtga ccgaggctgc ccaggttgaa gtgcgttatg gcgaaaccct gcgcgcattg
      901 ccgccgcggc cgaccagcta taccgtctat ttcgagaccg gcaccgacac gatgacgcca
      961 gcgtcggcgc aggccttcgt cggcgtcaag cgggaaatcg ccgaacggcc ggcggtcgag
     1021 gtcatggtca tcggccacac cgaccgcgtc ggctcgccgg ccgagaacga tccgctgtcg
     1081 cggcagcgcg ccgaactggt gcgcgatcaa ctggtggccg aaggggtgcc ggcggagaag
     1141 ctggaagtcg ccgggcgcgg cgaacgcgaa ccgctggtgc cgaccgccga cgacgtcgac
     1201 gagccgcgca accgccgggt cgagatcagc atccgctgag ccgcgggccg ttccagcgca
     1261 gcacgagcag ggtcaggtcg tcggcggcgg gttcgccggc ctcgaactgg cggacgctgt
     1321 cgcggatctg ccggcagacg gtgccgggtt cggtttccgg tgtccgtgcc agacaggctt
     1381 ccaggccggc gtggccgaat agctggccgt cgcgagtggc ttcgctgacg ccgtcggtga
     1441 acaggcagag caggtcgcct gcttgcagcc ggatgctgcc gctggcgtag gggtaatcgc
     1501 ccagcgcgca gagcggcggg cctgaaatct ccgccatcgg cagcggctgg agttcgccgt
     1561 tgcgcttgag gagcggcgcg tcatggccgg cgcagacata atccagccgg ccgtcgtcgg
     1621 catcgagaat ggcgatgaag gcggtgacga aaagcagcgc cgtgttgttg cggtcgagct
     1681 cgtgttcgag gtcgcggatg gcggcgccga gatcgtcatg gcgccgggtc agcgtgttgc
     1741 ccagggtttt cgagatggcc atgaacaggc tggcgggaac gcctttgccg gacacgtcgc
     1801 cgatggcgat gcagagacgg cgcgtgtcga gcatgaagca gtcgtagtag tcgccgccga
     1861 ccgcctgtgc cggttcgagg atggcggcaa gggcaaagcg tgtttcgtcg gcaaacagac
     1921 gttcggggtc gggcagcagc cccatctgga tgcgccgcgc gctgtcgagt tcgccggcga
     1981 tgcgcgccgc ctgttcgcgg cttgcctgga gttgccgttc cgccgcctgg tgccgctcgt
     2041 cggcgcggat cagggtgttg ccgagcaggg ccatgaacac cggcccgagc agcagggccg
     2101 gtgtgttgcc gtcgaccagc cagcgtccgc tgaagtaggc gagatggccg gcgagcagca
     2161 ggctgccgcc gacggcggcg aaacccagca ccgcgtagcg cggccgcaag gccggtacgc
     2221 tgccgatcag cagcaggccg ccggcgagca gggcggccag ttcgagcatc ggcatccagt
     2281 ccggccggcg cagggcgtcg ccggcaagca ggctctcgat gacctggacg tgcacgtcgg
     2341 cgccgggcag gctgtcgcca agcggcgtga tgacgcggtc ctggaggccg atgctgttga
     2401 agccgacgat caccaggcgt ccggcgaagg tttccggcgg gtgcttgccg gccaggatat
     2461 cggcggccga caggtagtaa tgcgaactgg cgcgcccgaa atgcagcagc agttcgccgt
     2521 tggcctgggt cggcagccgg tagtcgccga ggcgcagcgc cgccatgccc ttgccgccgg
     2581 cctcgatggc gacggcgccg ccgcccagcg cctgtctgac catctcgagc ggcaacgaga
     2641 gatagggttg ctcgtcgatc aacgcaagcg tcggcacgcg gcgcaggatg ccacggtcgt
     2701 ggccgccgtt gcgggcgtcc ggcgtggcgt tgatgaagcc ttcgccaccc gcagccgcct
     2761 gcagctcagg gcggctggcg agggcactgg cgaagcgggg caacaggcgg tcggcccccg
     2821 gttcgatggc gatttgcggc agcggatggc gcggcagcct ggcgccggcc agcgccaggg
     2881 gcaggccgac cgtggcgaga acggtcggcg cccggccgat gacctcggca aactgctggt
     2941 caggatcggg caggtggctg agcagcgcgg gatcgaggcc gggcaggcgg cgggcgagca
     3001 gggctggggc gtgctggtcg gcttcggcaa aaatgaaatc gaaaccgacg gcga
//
LOCUS       DAOSDX010000290         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11356_length_3052_cov_3.383383, whole genome shotgun sequence.
ACCESSION   DAOSDX010000290 DAOSDX010000000
VERSION     DAOSDX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11356_length_3052_cov_3.383383"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLN02_07380"
     CDS             complement(<1..605)
                     /locus_tag="PLN02_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI52186.1"
                     /translation="MNATRNQLVFGRVLHFLADPGEQDAADSYEYFPDGALWIVAGRI
                     AAAGPRTAVEAGIAPAERAAAEVIDYGKQLILPGLVDTHCHYPQSAVIASFGRQLLDW
                     LNDYTFPAETAFADPEVAARTADYFLDRLLAHGTTTAAVFSTVHAGSVDAFMAGAEKR
                     HLRMLTGKVMMDRHAPPALCDTVADAERDCLALIERWHGQGR"
     gene            complement(607..1968)
                     /locus_tag="PLN02_07385"
     CDS             complement(607..1968)
                     /locus_tag="PLN02_07385"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOI52187.1"
                     /translation="MTTLLLKDADVLVTMDAARREIPGGGVFIRDGVIEAVGPSAELP
                     TTADQVISLAGTVALPGLVNTHHHMYQSLTRAIPGAQDAELFGWLRRLYPIWAGLTPE
                     MIRVSTLTAMAELILSGCTTSSDHLYLYPNGSRLDDSIEAAATIGMRFHAARGSMSVG
                     ESQGGLPPDRLVENEASILKDTQRLIETWHDPQRHAMQRIVVAPCSPFSVSRELMRDA
                     AELARSYGVSLHTHLAENDNDVAYSREKFGMTPAEYAESLGWVGHDVWHAHCVKLDTP
                     GINLFARSGTGVAHCPCSNMRLASGIAPIPALRAAGVNVGLGVDGCASNDAAHLLGEA
                     RQAMLLARVGHGPAAMSARQALELATLGGAKVLNRDDIGALAPGMSADLVAFPTGGLD
                     LAGAAVHDPVAALVFCTPQTVRHSIINGKPVVSDGHLLPLDLPPLVAEHNRLARQLAN
                     AAH"
     gene            complement(1965..2885)
                     /locus_tag="PLN02_07390"
     CDS             complement(1965..2885)
                     /locus_tag="PLN02_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI52188.1"
                     /translation="MLDYLPLILAGTLAAATPLIFAGLGELVAERSGVINLGVEGMML
                     IGAIAGFAAAAETGHGVAIGLPAGALAGAAAAAVFGFFALSLAANQAACGLALTIFGI
                     GLSAFIGQHYVSYSLPGLQPIAIPLLSDIPLLGPALFKQDWVVYLSLLAFALVAWTLA
                     KTRLGLLLKAVGEAPDAAHAIGYPVIAIRYGAVIFGGAMAGIAGAYLSTVYTPLWVQN
                     MSAGRGWIAVALVVFATWKPTRLMLGAYLFGFVTILQFHAQGLGIEVPVQLLAALPYL
                     ATIVVLVLISRDRKLLQLNLPASLGKPFLP"
BASE COUNT          487 a          923 c         1201 g          441 t
ORIGIN      
        1 cggccctggc cgtgccagcg ctcgatcagt gccaggcagt cgcgttcggc gtcggcgacg
       61 gtatcgcaca gcgccggcgg cgcgtggcgg tccatcatga ccttgccagt caacatgcgc
      121 aggtggcgtt tttccgcgcc ggccatgaag gcgtcgaccg agccggcatg cacggtcgag
      181 aagacggcgg cggtggtcgt gccgtgggcg agcaggcggt cgaggaagta atcggcggtg
      241 cgcgcggcga cttccgggtc ggcgaaggcg gtctcggccg ggaaggtgta gtcgttgagc
      301 cagtcgagca gttgccggcc gaaggaggcg atgactgccg actgcgggta gtggcagtgg
      361 gtgtcgacca ggccgggcag gatcagctgc ttgccgtagt cgatgacttc ggcggcggcg
      421 cgttcggccg gcgcgatgcc ggcttcgacc gcagtgcgcg ggccggcggc ggcgatacgg
      481 ccggcgacga tccacaatgc gccgtcggga aagtactcgt agctgtcggc ggcgtcctgc
      541 tcgccgggat cggcgaggaa gtggaggacg cggccgaaga ccagttgatt gcgggtggcg
      601 ttcatgtcag tgcgcggcgt tggcgagctg gcgcgccaac cggttgtgtt cggcaaccag
      661 cggcggcagg tcgagcggca gcaagtggcc gtcgctaacg accggtttgc cgttgatgat
      721 cgaatggcgc acggtctgcg gcgtgcagaa gaccagcgcg gcgaccgggt cgtgcacggc
      781 agcgccggcc aggtcgaggc cgccggtcgg gaaggcgacg aggtcggcgc tcatgcccgg
      841 ggcgagggcg ccgatgtcgt cgcggttgag caccttggcg ccgcccagcg ttgccagttc
      901 cagtgcctgg cgggcgctca ttgcggcggg gccatggccg acgcgggcca atagcattgc
      961 ctgccgggct tcgccgagca ggtgggcggc gtcgttcgag gcgcagccgt cgacgccgag
     1021 gccgacattg acgccggcgg cgcgcagcgc cggaatcggc gcgatgcctg aagccaggcg
     1081 catgttcgag cacgggcagt gggcgacgcc ggtgccgctg cgggcaaaaa gattgatgcc
     1141 cggcgtgtcg agcttgacgc agtgggcgtg ccagacatcg tggccgaccc agccgagcga
     1201 ctcggcgtat tcggccgggg tcatgccgaa cttttcgcgg ctgtaggcga cgtcgttgtc
     1261 gttctcggcc agatgcgtat gcagcgaaac accatagctg cgcgccagtt cggcggcgtc
     1321 gcgcatcagt tcgcggctga ccgaaaacgg cgagcagggc gcgacgacga tgcgctgcat
     1381 ggcgtggcgc tgcgggtcgt gccaggtttc gatcaggcgc tgggtgtcct tgaggataga
     1441 cgcttcgttt tcgacaaggc gatccggcgg caggccgccc tggctttcgc cgacgctcat
     1501 gctgccgcgc gcggcgtgaa agcgcatgcc gatggtggcg gcggcttcga tggaatcgtc
     1561 gaggcggctg ccgttcgggt aaaggtagag atgatcgctg gaggtggtgc agccggagag
     1621 gatcagctcg gccatcgcgg tcagcgtcga cacgcggatc atttccggcg tcagcccggc
     1681 ccagatcggg tagagccggc gcagccagcc gaacagttcg gcgtcctggg cgccggggat
     1741 ggcgcgggtc aggctctggt acatgtggtg gtgggtgttc accaggccgg gcagggcgac
     1801 cgtgccggcc agcgagatga cctggtcggc ggtggtcggc agctcggcgg acgggccgac
     1861 cgcctcgatc accccgtcgc ggatgaagac gccgccaccg gggatttcgc ggcgggcggc
     1921 gtccatggtg acgaggacgt cggcgtcctt gagcagcagc gtggtcatgg caggaagggc
     1981 ttgcccagcg aggccggcag gttcagttgc agcaacttgc ggtcgcgcga gatcagcacc
     2041 aggacgacga tggtcgccag gtaaggcagc gccgccagca gttgcaccgg cacctcgatg
     2101 ccgaggccct gggcgtggaa ctgcaggatg gtgacgaagc cgaacaggta ggcaccgagc
     2161 atcagccggg tcggcttcca ggtggcgaag acgaccagcg cgacagcgat ccagccgcgc
     2221 ccggcgctca tgttctggac ccacagcggc gtatagaccg tcgacagata ggcaccggcg
     2281 atgccggcca tggcgccgcc gaagatcacc gcgccgtagc ggatggcgat caccggatag
     2341 ccgatcgcgt gggcggcgtc cggcgcttcg ccgaccgcct tgagcagcag gccgagccgg
     2401 gttttggcca gggtccaggc gaccagcgcg aaggcaagca gcgacaggta gaccacccag
     2461 tcctgcttga acagcgccgg cccgagcagc gggatgtcgc tgagcagcgg gatggcaatc
     2521 ggctgcagcc cgggcaggct gtagctgacg taatgctggc cgatgaaagc cgagaggccg
     2581 atgccgaaga tggtcagggc gagcccgcag gcggcctggt tggcggccag tgacagcgcg
     2641 aagaagccga acacggcggc ggccgcggcg ccggccaggg cgccggccgg caggccgatg
     2701 gcgacgccgt ggccggtttc ggcggcagcg gcgaaaccgg cgatggcgcc gatcagcatc
     2761 atgccttcga cgccgaggtt gatgacgccg ctgcgttcgg caaccagttc gccgaggccg
     2821 gcgaagatca gcggggtggc ggcggcgagg gtgccggcga ggatcagggg caggtagtcg
     2881 agcatcgcgg tctccggtca gcgggcgcgg cggcgcaggc ggttgtcgat gaaggcgtcg
     2941 gcgccgagca ggaagaagag cagcatgccc tggaagatgc cggtgatcgc cgccggcatc
     3001 tgcaggctca gttgcgccgc ttcgccgccc agatagatca gcgccatcag gc
//
LOCUS       DAOSDX010000291         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11382_length_3045_cov_3.507692, whole genome shotgun sequence.
ACCESSION   DAOSDX010000291 DAOSDX010000000
VERSION     DAOSDX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11382_length_3045_cov_3.507692"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLN02_07395"
     CDS             <1..915
                     /locus_tag="PLN02_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOI52189.1"
                     /translation="LPLLVLSGPPASVSTPLIHMVETGALQAVADKVEFRPWKDPDQL
                     RVMALGKQADFVAMPTNVAANLHNRGVPLRLINVSTWGVLWMVSRDAGAKTLADFRGK
                     EIAMPFRADMPDIVFELLARRQGLDAKKDFKLRYVASPLDAMQLLVTRRVDHALLAEP
                     AVSMALRKTQSFPLSLIAPDLHRSVDLQQEWGRLFKRETRIPQAGIVALGAAAADPAL
                     QAAVLAAYARSLAWCQQNPLQCGAMVAKRVDLLSPEAVADSLAVSQMRSLPTAEARAE
                     LEFFLGQLHAANPALIGGKLPGPAFYAR"
     gene            912..1733
                     /locus_tag="PLN02_07400"
                     /pseudo
     CDS             912..1733
                     /locus_tag="PLN02_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336605.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            1745..2467
                     /locus_tag="PLN02_07405"
     CDS             1745..2467
                     /locus_tag="PLN02_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI52190.1"
                     /translation="MHHRFAYQRVLEDIDLCVQAGQAVALVGPSGCGKSTLLNLAAGL
                     LSPWEGRIDSSFEQAAMMFQQPRLLPWKRTLDNIALGLKARRTPIAERQTRARQLAHR
                     MGLDDDALDKFPAALSGGMQSRAALARAFAVSPDLLLLDEAFAALDIGLKSELYGLLA
                     RERAAAGSAVLMITHDLMEALRLADRILVMSGNPGRIVAEHRVATPVGERDDAWVFAR
                     TAEFIRWPDIRAAFALPPLAAT"
     gene            2464..>3045
                     /locus_tag="PLN02_07410"
     CDS             2464..>3045
                     /locus_tag="PLN02_07410"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOI52191.1"
                     /translation="MKNRTFPGLSAALWLCPCRPFFLLTAAHACIAMAWWLGNLAGLL
                     ALPELPGGSVVWHIHEMLFGFATAALAGFLLTAVVEFTGTPPAPRQTVQILLLLWAGG
                     RLAYLLAGWLGVWPAALCDLAFLGLLLVKTGAPLWRDPQRRHLAFFHALAALTGSHAG
                     FYLALARDGDAMAWLRLAIGLLMMLIVVAMSRIS"
BASE COUNT          417 a         1166 c          936 g          526 t
ORIGIN      
        1 ctgccgctgc tggtgctgtc cggcccaccg gcctcggtct cgacaccgct catccatatg
       61 gtcgagaccg gcgccctgca ggcggtcgcc gacaaggtcg aattccgccc ctggaaagac
      121 ccggaccagt tgcgcgtcat ggccctcggc aaacaggcgg atttcgtggc gatgccgacc
      181 aatgtcgccg ccaacctcca caaccggggc gtcccgctgc gcctgatcaa cgtttcgacc
      241 tggggcgtgc tgtggatggt ctcgcgcgac gccggggcga agacgctggc tgatttccgc
      301 ggcaaggaaa tcgccatgcc cttccgcgcc gacatgccgg acatcgtctt cgaactgctg
      361 gcgcgccggc agggcctgga tgcgaaaaag gatttcaagc tgcgttacgt cgcctcgccg
      421 ctcgacgcca tgcagttgct ggtgacgcgg cgcgtcgacc acgccctgct cgccgagccg
      481 gcggtgtcga tggcgctgcg caagacgcaa tccttcccgc tcagcctgat cgcccccgat
      541 ctccaccgca gcgtcgacct gcagcaggaa tggggacgcc tgttcaaacg cgagacgcgc
      601 attccgcagg ccggcatcgt cgccctcggc gcggcggccg ccgaccccgc gttgcaggcg
      661 gcagtgcttg ccgcctacgc ccggtcgctg gcctggtgcc agcaaaaccc gctgcaatgc
      721 ggcgcgatgg tcgccaaacg cgtcgacctg ctgagcccgg aagccgtcgc cgactcgctg
      781 gcagtcagcc agatgcgttc gctgccgacg gcggaagccc gcgccgaact cgaattcttc
      841 ctcggccagt tgcacgccgc caacccggcg ctgatcggcg gcaagctgcc cggccccgcc
      901 ttctacgccc gatgagccgc caggctggac gggcgcagca agtgctcgag gcgctcgccg
      961 ctagcggcca ttacctgtgg cacggctggg gttcgctggc cagtctctgc ctcttcctcg
     1021 ccgcctggga actgaccgca cagagcttcg ggccgttgat cctgccgacg ccgatggccg
     1081 ccttcgccac cctcggcggc ctcctcgacg gcggcggcgc ctggccggaa atcgccgtca
     1141 cggcgcgccg ggcgctcgcc ggcttcgcgc tttcggtcgg cgtcggcagc ctgctcggtc
     1201 tggccgccgg cgtttcgatg acagcctcga tgatggcccg gccgatcatc accgtgctcg
     1261 ccggcatgcc gccgatcgcc tggctgatcc tcgccctgct ctagttcggc gccggtgacg
     1321 gaacgccggt attcaccgtc ttcatcgcct gcttcccgat cgtcttcgtc ggcgccatgc
     1381 agggcacgcg caccctcgac ggccagttgc gggaagtggc gcagatgttc ggtctctcgc
     1441 cctggctgaa gctgaccgac gtctatctgc cgcacgtcgt ttcctatctc ttcccggcgt
     1501 ggatcgcggc gctcggcatg tcgtggaaga tcgtcgtcat ggccgaacta ctgtccaccg
     1561 aggacggcat cggcgccgcg ctggccgtct cgcgcagcca cctcgacacc gccgcctcga
     1621 tggcctggat cgtcgccctg gtcggcaccc tgctggccat cgaatacctt ctgctcgaac
     1681 cgctcaagcg ccgcgtcgag tcctggcggg agttcgcatc atcaagcaac tgaagatttc
     1741 ggcggtgcac caccgcttcg cctaccagcg cgtgctcgaa gacatcgacc tctgcgtgca
     1801 agccggtcag gccgtcgccc tggtcggccc ttccggctgc ggcaagagca ccctgctcaa
     1861 cctcgccgcc ggcctgctca gcccctggga aggacgcatc gacagcagct tcgaacaagc
     1921 cgcgatgatg ttccagcaac cacgcctgct cccctggaaa cgcacgctcg acaacatcgc
     1981 cctcggtctc aaggcgcgcc ggacaccgat tgccgaacgg cagacccgcg cccgccagct
     2041 ggctcaccgg atgggtctcg acgacgacgc tctcgacaag tttcccgccg ccctttccgg
     2101 cggcatgcag agccgcgccg cgctggcccg cgcctttgcc gtctcgcccg acctgctgct
     2161 cctcgacgag gccttcgccg ccctcgacat cggcttgaaa agcgaactct acggattgct
     2221 cgcgcgcgag cgcgcagcgg ccggcagcgc cgtgctgatg atcacccacg acctgatgga
     2281 agccctccgc ctggccgacc gcatcctggt gatgagcggc aaccccggcc gcatcgtcgc
     2341 cgaacaccgg gtcgccacgc cggtcggcga acgcgacgac gcctgggttt tcgcccgtac
     2401 cgccgaattc atccgctggc cggacatccg cgccgccttt gccctgccgc cgctggccgc
     2461 cacatgaaaa accgtacttt tcccggacta agcgcagccc tctggctctg cccctgccgc
     2521 cccttcttcc tgctcaccgc cgcccatgcc tgcatcgcca tggcctggtg gctgggcaac
     2581 ctggccggtc tgctagcgct gcccgagttg ccgggcggca gcgtcgtctg gcacatccac
     2641 gagatgctgt tcggcttcgc caccgcggcg ctggctggct tcctgctcac cgccgtggtc
     2701 gaattcaccg gcaccccgcc ggccccccgg caaaccgtgc agattctgct cctcctctgg
     2761 gccggcggtc gcctcgccta cctgctggcc ggctggctcg gcgtctggcc agccgccctg
     2821 tgcgacctcg ctttcctcgg actgctgctg gtcaaaaccg gcgcccccct gtggcgcgac
     2881 ccgcaacgcc gccacctggc gttttttcac gcactggccg cgttgaccgg gagccacgcc
     2941 ggcttctacc tggcgctggc gcgtgacggc gacgccatgg cctggctgcg cctggccatc
     3001 ggcctgctga tgatgctgat cgtcgtcgcc atgtcgcgca tctcg
//
LOCUS       DAOSDX010000292         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11408_length_3039_cov_3.593834, whole genome shotgun sequence.
ACCESSION   DAOSDX010000292 DAOSDX010000000
VERSION     DAOSDX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11408_length_3039_cov_3.593834"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLN02_07415"
     CDS             complement(<1..663)
                     /locus_tag="PLN02_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI52192.1"
                     /translation="MKGVELSADDPLDVRRQKLARIILDSMYQFLGLLDVDGRVIEIN
                     QAALDGAGIKLEEVVGKPFWEARWWALSAATQERVRTMVAEAASGQFVRCDIEIYGES
                     SGRRTIVVDFSLTPIFDDSGRVAFLLPEGRNISEKIAIEAELTRRNNEVHAALEKLRA
                     LDGFKTKFFANVSHELRTPLTLILGPVNQLLQEAGRSEREQSRLLTIKRNAQALLQQV
                     NDL"
     gene            complement(817..2193)
                     /locus_tag="PLN02_07420"
     CDS             complement(817..2193)
                     /locus_tag="PLN02_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOI52193.1"
                     /translation="MIQQKLAAGLAGTAIMLGGQAAWATDVFRLEGIGAVSRAMGGTA
                     VAHDVGPGGMLTNPATLSLMPGEQQAMVGFDLVTTDIEVRDEATGAKAHSGTHSRNRG
                     PYVAPQAAYSRRLGNLVVGVGAFAQGGLGTEYGSRSFLSNGTGGAPTGLENSSRLLVL
                     DIPLAASYRVSEALTVGASLDAMWQGLNLDLLLGADQVGSLIAGGRATGALVPVLGGI
                     PALQGAHFSLTKNKWLGSGVDAWGFAGRLGMLYQVSPATRLGAAYTVKSKIDDLQGSA
                     TLTAVSGLAGQIPLAGKIKLKDFQMPARLDLGLHHRLTPQWEVAADLSRVFWEDVMKD
                     IKVAFVANAGGDLNILLPQNYKDQTVFAVGTAYALTPQWTLRAGARVASQALRGNTLF
                     AVIPATPTKHLSLGFTYAFSPSSKIDFAWSHALREKMANRSLPNTSDPISVAHSQDNA
                     TLNYSLSF"
     gene            complement(2255..>3039)
                     /locus_tag="PLN02_07425"
     CDS             complement(2255..>3039)
                     /locus_tag="PLN02_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOI52194.1"
                     /translation="CQCRACGPITIAAPTAAEYVPLLAPRRQRAQLAMLRDITHDIRE
                     FRFVSDGPADFLPGQYAMLELPGVSGSRAYSMANTPNEQREWHFQVRRVPHGQGSHVL
                     FDGLAVGDRIGLDGPYGMAWLRTASLRDIVCVAGGSGLAPMLSIARGAVDAGLLGERT
                     LYFFYGARTPRDVCGGDLLAKLGAGERIRFIPVVSLPGDDGAWTGATGYVHDQLLPQL
                     PARLAHYEFYFAGPPPMTSALQELLMVGHQVPFEQIHFDRFF"
BASE COUNT          467 a          978 c         1098 g          496 t
ORIGIN      
        1 cagatcgttg acctgctgca gcagagcctg ggcgttgcgc ttgatcgtca gcagccgcga
       61 ctgctcgcgc tcgctacggc ccgcctcctg cagcaactgg ttcaccgggc cgaggatcaa
      121 cgtcagcggg gtgcgcaact cgtgactgac gttggcgaag aacttggtct tgaagccgtc
      181 gagcgcccgc agcttctcca gcgcggcgtg cacctcgttg ttgcggcggg tcagttcggc
      241 ctcgatggcg attttttccg agatgttgcg gccttccggc aacaggaagg cgacccgtcc
      301 gctgtcgtcg aagatcggcg tcagcgagaa gtcgacgacg atcgtccggc ggccgctcga
      361 ttcgccgtag atctcgatgt cgcagcgcac gaactggccg ctcgccgctt ccgccaccat
      421 cgtccgcacc cgttcctggg tggccgccga cagcgcccac cagcgggctt cccagaacgg
      481 cttgccgacc acttcctcga gcttgatccc ggcgccgtcg agcgccgcct ggttgatctc
      541 gatgaccctg ccgtcgacgt cgagcaggcc gaggaactgg tacatcgaat cgaggatgat
      601 ccgcgccagc ttctggcgcc ggacatcgag ggggtcgtcg gccgacagtt cgacgccctt
      661 cacgggcgcc tccctggcgg gtcttcccgg atcggtgata ttggccatga acgcattgca
      721 aggactaacg gaaatgaagc atccaacact ctggaaaaaa aagcgaggca agcctcgctt
      781 gaaaagcccc gaaggaggga tggggctggg gcttgatcag aaactgaggc tgtagttgag
      841 cgtcgcgttg tcctgggaat gcgcgacgct gatcgggtcc gaggtgttcg gcaggctgcg
      901 gttggccatc ttttcgcgca gcgcgtggga ccaggcgaag tcgatcttgc tgcttggcga
      961 gaaggcgtag gtgaagccga gcgacaggtg cttggtcggc gtcgccggga tcaccgcgaa
     1021 cagcgtgttg ccgcgcagcg cctgcgaagc gacgcgggcg ccggcgcgca gcgtccattg
     1081 cggcgtcagc gcgtaggcgg tgccgacggc gaatacggtc tggtccttgt agttctgcgg
     1141 cagcaggatg ttgaggtcgc cgccggcgtt ggccacgaaa gccaccttga tgtccttcat
     1201 gacgtcctcc cagaacaccc gcgacaggtc ggcggcgact tcccattgcg gcgtcagccg
     1261 gtgatgcagg ccgagatcga ggcgggccgg catctggaaa tccttgagtt tgatcttgcc
     1321 ggcgagcgga atctgcccgg ccagtccgga aacggcggtc agcgttgccg agccctgcag
     1381 gtcgtcgatc ttgctcttga ccgtgtaggc ggcgcccagg cgggttgccg ggctcacctg
     1441 gtagagcatg ccgaggcggc cggcgaagcc ccaggcgtcg acgccgctgc ccagccattt
     1501 gttcttggtc aggctgaaat gcgccccctg cagcgccggg atgccgccca gcacgggcac
     1561 cagggcgccg gtcgcgcggc cgccggcgat caggctgccg acctggtcgg cgccgagcag
     1621 caggtcgagg ttgagcccct gccacatggc gtcgaggctg gcgccgacgg tcagcgcctc
     1681 ggaaacgcga tagctggcgg cgagcgggat gtcgaggacg agcaggcggc tggagttttc
     1741 gagcccggtc ggcgcgccgc cggtgccgtt ggacaggaag ctgcggctgc cgtactcggt
     1801 gcccagcccg ccctgggcga aggcgccgac gccgacgacc aggttgccga ggcgccggct
     1861 gtacgctgcc tgcggcgcga cgtagggacc gcggttcctg ctgtgcgtgc cggaatgcgc
     1921 cttggcgccg gtggcctcgt cgcggacctc gatgtcggtg gtcaccaggt cgaagccgac
     1981 catcgcctgc tgttcgcccg gcatcagcga cagcgtcgcc gggttggtca gcatgccgcc
     2041 ggggccgacg tcgtgggcga cggcggtgcc gcccatggcg cgcgagacgg cgccgatgcc
     2101 ttcgagccgg aagacatcgg tcgcccaggc ggcctggccg cccagcatga tggccgtgcc
     2161 ggcgaggccg gcagccagtt tctgttggat catgtttttt ctcctcttgt cgtttgttgt
     2221 gtttgcctgt tgcggcaagt ccccgctgac cgcgtcagaa gaaacggtcg aaatgaatct
     2281 gttcgaacgg cacctgatgg ccgaccatca gcagttcctg cagcgccgag gtcatcggcg
     2341 gcggcccggc gaaatagaac tcgtagtggg cgagccgcgc cggcagttgc ggcagcaact
     2401 ggtcgtgcac gtagccggtg gcgccggtcc aggcgccgtc gtcgccgggc agcgaaacca
     2461 ccgggatgaa gcggatacgc tcgccggcgc cgagcttggc gagcaggtcg ccgccgcaga
     2521 cgtcgcgcgg cgtgcgggcg ccgtagaaga agtacagcgt gcgttcgccg agcaggccgg
     2581 cgtcgaccgc gccgcgggcg atcgacagca tcggcgccag cccggaaccg ccggcgacgc
     2641 agacgatgtc gcgcagcgac gcggtgcgca gccaggccat gccgtaggga ccgtcgagac
     2701 cgatgcggtc gccgacggcg aggccgtcga agaggacatg gctgccctgg ccgtgcggca
     2761 cgcgacggac ctggaagtgc cactcgcgct gctcgttcgg cgtgttggcc atcgagtagg
     2821 cgcgcgatcc gctgaccccc ggcagctcca gcatcgcgta ctggccgggc aggaagtcgg
     2881 ccgggccgtc gctgacgaag cggaattcgc ggatgtcgtg ggtgatgtcg cgcagcatcg
     2941 ccagttgcgc gcgctgccgg cgcggcgcca gcagcggcac gtattcggcg gcggtcggcg
     3001 cagcgatggt gatcgggccg caggcccggc actggcagg
//
LOCUS       DAOSDX010000293         3038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11415_length_3038_cov_3.545089, whole genome shotgun sequence.
ACCESSION   DAOSDX010000293 DAOSDX010000000
VERSION     DAOSDX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11415_length_3038_cov_3.545089"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..277
                     /locus_tag="PLN02_07430"
     CDS             86..277
                     /locus_tag="PLN02_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52195.1"
                     /translation="MNNLLGLLRELRLMKDQPSHRFYAVWLLILLFMGRGFVLIVLIL
                     GGGFVIKPALVMGLLSRFF"
     gene            294..947
                     /gene="cas5c"
                     /locus_tag="PLN02_07435"
     CDS             294..947
                     /gene="cas5c"
                     /locus_tag="PLN02_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOI52196.1"
                     /translation="MDKFCLEVSGDFACFTRPEMKVERVSYDVITPSAARSVFEAILW
                     KPAIRWEVRRIEVLKPIKWISVRRNEVSAKASVRNAQTAMNAGQGNLALYIEDDRQQR
                     AGLFLRDVAYRLHAELLPVGDDARANPAKYREMFQRRAEKGQCVNQPYLGCREFAARF
                     RLVTDPDSEPPALAESRDLGWMLYDMDFAAAGDPKPRFFRADLQNGVIDLTQAQVTA"
     gene            944..2683
                     /gene="cas8c"
                     /locus_tag="PLN02_07440"
     CDS             944..2683
                     /gene="cas8c"
                     /locus_tag="PLN02_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOI52197.1"
                     /translation="MILQALYDYYQRKAVDPDPARRLPSFGLEDKEIPFIVELTAEGR
                     PLGIVDTRQGDGKKKQARRYQVPKGVKRSSGVAANLLWDTAEYALGVDTKGKPERVAE
                     QHAAFRQRIAELPEAARQDAGILALGNFYANFGQAALVADPAWPEIVEGNPVVTFRLH
                     NDGDLICQRPAVIAGRVDVAEEGGKPGFCLVTGQAASAERLHTVIKGVWGAQSSGATL
                     VSFNLDAFASYNKEQGDNAPVSPLAAFAYTTALNHLLDRNSRQRIQVGDASTVFWAQK
                     DDAEIEEGFAAIFGERDDPDARAEQVRALLGAVQSGQFDGGRGDKLFYVLGLAPNAAR
                     VSVRFWHAAPLHEIARQIRQWFADLKVARSAKDPEYPSLFRLLAACAQQGKADNIPPN
                     LGGDIMRAILSGAPFPATWLNAAVLRCRAEQNVTYLRAAAIKASLNRLQRFQPNQSLD
                     KELSDMLDLENTSPAYRLGRLFAALEKIQEEASPGLNATIRERYYGAASSTPVAVFTT
                     LLRLKNHHLAKLTNRGRATNFERLLGEIMGGLNDFPKHLSLPEQGRFALGYYHQRQDF
                     FSKSESSQSMQST"
     gene            2695..>3038
                     /locus_tag="PLN02_07445"
     CDS             2695..>3038
                     /locus_tag="PLN02_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOI52198.1"
                     /translation="MSLNNRYDFVLVFDVRDGNPNGDPDAGNLPRLDAESGHGLVTDV
                     SLKRKVRNFVGLVKGETPPFDIYVKEKAVLNQTHEKAYVAVGAEEELKGDKKRKGSGD
                     TVDKARQWMCTN"
BASE COUNT          605 a          924 c          917 g          592 t
ORIGIN      
        1 ggtgcgtagt gtattcaacg acaactaaac cattgagaac gtttagtgta tagtcggcat
       61 cgtttcctgg ccaggggcga tacccgtgaa caatttactt ggactcttgc gcgaactgcg
      121 attgatgaag gatcagcctt ctcatcgctt ttacgcggta tggcttctga ttttgctgtt
      181 catgggtaga ggcttcgtat tgatcgtttt gatactcggt ggaggtttcg tgatcaagcc
      241 ggccttagtg atgggcttgc tgtcacgctt cttctgaaag aaaggagttg agcttggata
      301 agttctgtct tgaagtatct ggtgacttcg cctgcttcac ccggccggaa atgaaggtcg
      361 agcgggtgtc ttacgacgtg atcacgccat cggcggcgcg ttcggtgttc gaggccattc
      421 tgtggaaacc ggcgatacgc tgggaggtgc gccgcatcga ggtcttgaag ccgatcaaat
      481 ggatcagcgt gcggcgtaac gaggtgtcgg ccaaggcgtc ggtgcgcaat gcccagacgg
      541 cgatgaatgc cgggcagggc aatttggcgc tgtatatcga ggacgaccgc cagcagcggg
      601 ccgggctgtt cctgcgcgat gtggcttatc gcctgcatgc cgaactgctg ccggtcggtg
      661 acgatgcgcg ggcgaatccg gccaagtacc gggaaatgtt ccaacgccgg gccgagaaag
      721 ggcagtgcgt caatcagccc tatctcggtt gccgcgaatt cgccgcccgt ttccggctgg
      781 tcaccgatcc ggacagcgag ccgccggccc tggctgaaag ccgcgatctg ggctggatgc
      841 tctacgacat ggattttgcg gcggcgggcg acccgaaacc gcgctttttt cgcgccgatc
      901 tgcaaaacgg ggtcatcgac ctgacacaag cgcaggtgac ggcatgatcc tgcaagccct
      961 ctacgattac taccagcgca aggccgtcga tcccgatccg gctcgccgcc tgccgtcctt
     1021 cgggctggaa gacaaggaaa ttcccttcat cgtcgagttg accgccgaag gccggccgct
     1081 cggcatcgtc gatacccgcc agggcgatgg caagaagaaa caggcgcggc gttaccaggt
     1141 gcccaaaggc gtcaagcgtt cctccggcgt ggcggccaac cttttgtggg ataccgctga
     1201 atacgccctg ggcgtcgata ccaagggcaa gcccgaacgg gtggccgagc aacatgcggc
     1261 tttccgtcag cggattgcgg aattacccga agcggcccgc caggatgccg gcatcctggc
     1321 cttgggtaac ttctacgcca acttcggaca agcggccttg gtcgccgatc cggcctggcc
     1381 ggaaatcgtc gaaggaaatc ctgtcgttac gttccggctg cacaacgatg gcgacttgat
     1441 ctgccagcgc ccggcggtga ttgccggacg agtcgatgtg gccgaagaag gcggcaagcc
     1501 gggtttctgc ctggtgaccg gccaggcggc atctgccgaa cgcctgcata cagtgatcaa
     1561 aggcgtctgg ggcgcgcaaa gctccggcgc tacgctggtt tccttcaacc tcgacgcgtt
     1621 tgcctcctac aacaaggagc agggcgacaa tgcgccggtc agcccgctgg cagcctttgc
     1681 ctacacgacg gcgctcaatc acctgctcga ccgcaattcc cgccagcgca tccaggtcgg
     1741 cgatgcctcg acggtgttct gggcgcagaa ggatgatgcc gagatcgagg aaggtttcgc
     1801 cgccatcttc ggcgagcgcg acgatccgga cgcacgggcc gaacaggtgc gtgccttgct
     1861 cggtgccgtc caatcggggc agttcgacgg cggtcggggc gacaagctgt tttacgtgct
     1921 gggtttggcc cccaacgcgg cgcgggtttc cgtccgcttc tggcacgccg caccgctgca
     1981 cgaaatcgcc cgccagatca ggcagtggtt cgctgacctg aaagtcgcgc gcagtgcaaa
     2041 agacccggaa tacccgagcc tcttccgctt gctcgccgcc tgcgcccagc aaggcaaggc
     2101 cgacaacatc ccgcctaacc tgggcggcga catcatgcgg gcgattctct ccggcgctcc
     2161 gtttccggcc acttggctca atgcggcggt gttgcgttgc cgcgccgaac agaatgtcac
     2221 ctacctgcgc gctgccgcca tcaaggccag cctcaatcgt ttgcaacgct ttcaaccgaa
     2281 tcaatctctc gacaaggagt tgtccgacat gctcgattta gagaacacca gcccggccta
     2341 ccgacttggg cgcctgttcg cggcgctgga aaaaatccag gaagaagcca gccccggcct
     2401 caacgcgacc attcgcgaac gctattacgg cgccgcctcc agtacgccgg tggcagtttt
     2461 taccaccttg ctgcgcctca agaatcacca tctggcgaag ctcaccaatc gtggccgcgc
     2521 caccaatttc gagcgtctgc tcggcgaaat catgggcggc ctcaatgatt tcccaaaaca
     2581 tctgagcctg cccgagcaag gccgtttcgc cctcggctac taccaccagc gccaggactt
     2641 tttcagcaaa tccgaatcca gccaatccat gcaatccacc tagggagaat ccaaatgagc
     2701 ctgaacaacc gctacgactt tgtcctcgtt tttgatgtgc gcgacggcaa ccccaacggc
     2761 gacccggatg ccggcaacct gccgcgtctc gatgccgaat ccggtcatgg gctggtgacg
     2821 gatgtttccc tgaagcgcaa ggtgcgcaac tttgtcgggc tggtcaaagg cgaaacgccg
     2881 cctttcgata tctacgtgaa ggaaaaggct gtcctcaacc agacccacga aaaagcctat
     2941 gtcgcggttg gtgccgaaga agaactcaag ggcgacaaga agcgcaaggg gagcggcgat
     3001 acggtggaca aggcccgcca atggatgtgc accaattt
//
LOCUS       DAOSDX010000294         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11480_length_3022_cov_3.294574, whole genome shotgun sequence.
ACCESSION   DAOSDX010000294 DAOSDX010000000
VERSION     DAOSDX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11480_length_3022_cov_3.294574"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..847)
                     /gene="prfB"
                     /locus_tag="PLN02_07450"
                     /pseudo
     CDS             complement(1..847)
                     /gene="prfB"
                     /locus_tag="PLN02_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237878.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            1148..>3022
                     /locus_tag="PLN02_07455"
     CDS             1148..>3022
                     /locus_tag="PLN02_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI52199.1"
                     /translation="MQVLVHNLEGMVFRCAIDEHWTMHFVSDGSRELTGYCPDELEYN
                     KVVSYEAITHAEDRLRVRQRIVEAMNGVARYRVQYRIVHRDGSEKWVLERGAIVLDEE
                     GRRVLEGFVEDITDQVLSQRQLSDAEQRYRSIFEDSVVGMFQTSIDGRYIAANRALAQ
                     LYGYAGAAELIAGLSDIAGDLYVESGRRDEFETLMREHGRVSDFESEVYRRDGSRIWI
                     SEHAHVVRGPGGEPLYYEGTVEDITAQYHYRQQLEYQATHDPLTGLPNRNLLQDRLQQ
                     VVRLASRRGTKGSLAFVDVDNFKLVNDSLGHGAGDKLLVEVARRLKSCLRDSDTVARY
                     GGDEFVLILGDYDGLAETLHILHRIQDAIAEPITLDGQEVRVNCSIGVSIFPDDGVDL
                     DVLLRHADAAMHHAKKLGKGQFQFYTETLNVEARERLALDSALRRAIEEEALSVVYQP
                     KVNARGRACGFEALVRWESAEFGSVSPVRFIPLAEENGLIGQLTWFVLRQACHQAAAW
                     RAAGHPDLRIAVNISPSLFREKDLAALVGAVLEEAGLPAANLELEITEGMLMGDVQRS
                     LAVLGELKAMGVDIAVDDFGTGYSSLAYLKRFPIDILKVDRSFVLECDSGNGGLAITR
                     A"
BASE COUNT          510 a          931 c          963 g          618 t
ORIGIN      
        1 ttgcgaacgg tcgttctggc attgcacgac gatgccggtc gggacgtggg tcagacgcac
       61 ggcggagtcg gtcttgttga tgtgctggcc gcctgcgccg gaggcgcggt aggtgtcggt
      121 gcgcacgtcg gccgggttga tgtcgatttc gatcgaatcg tcgacttccg ggaagacgaa
      181 gaccgaagtg aagctggtgt ggcggcgggc gttggagtcg aacggcgatt tgcgcaccaa
      241 gcggtggatg ccggtttcgg tgcgcagcgt gccgtaggca tagtcgccgc tgaacttgac
      301 ggtggcgctc ttgatgccgg caacgtcgcc ttccgattct tccatcacct cgacgctgaa
      361 acccttcttt tcgccgaatt tcaggtacat gcgcagcagc atcgcggccc agtcctgggc
      421 ttcggtgccg ccggcgccgg cctggatttc caggaagcag ggcatggggt cggacgggtt
      481 gttgaacatg cggcggaatt cgagcgcggc gagcttcttt tccagctcgg cgttgtcggc
      541 ttcgacggcg agcagggtgt cgtcgtcgtt gtcttccttg cccatcgcga acagttcgcg
      601 gccgtcattg acgccgttgt ccacggcttc gaggacgagg acgatgtctt ccagcgattt
      661 cttttccttg cccagttcct gggcgcgctt cggatcgtcc cagatcttgg gatcttccat
      721 ttcctgattg aggagggtca ggcgctcgcg cttgtgatcg tagtcaaaga tacctccgca
      781 actcggcggc gcgttgcgcc aggtcgtcga gtttgttggc gatactgttg agatgttcgg
      841 cttccatgtc gattcaattc attcaaatgc aaaacgcgga ttatacattg accgggtagc
      901 gtgcccggcg ccactcagcg gggaatcgcc gcaccaggat ggggcgcgat tttgtcgttt
      961 ttccgctgct gagtcgcaaa ttcgctcgaa ttcgcagctt ctgtggttgg caagtctctt
     1021 gcttgacagt gtttcggatt aaccgagatt tacgattgtg aacgaccagg catcttcccc
     1081 cggctttctt cccgtactgc gcaaacgccg cgaatcggtc tggaacagga tcgacagcga
     1141 gcgcgtcttg caggtcctgg tccacaacct cgagggcatg gttttccgtt gcgccatcga
     1201 cgagcattgg acaatgcatt tcgtcagcga cggcagccgc gagctgaccg gctattgccc
     1261 ggatgaactc gagtacaaca aggtggtgtc ctacgaggcg atcacgcatg cggaggatcg
     1321 cctgcgggtg cgtcagcgca tcgtcgaagc gatgaacggc gtcgcccgtt accgggtgca
     1381 ataccggatc gtgcaccgcg atggttcgga gaaatgggta ctcgaacgcg gcgccatcgt
     1441 gctcgacgaa gaggggcgcc gggtgctcga aggcttcgtc gaagacatca ccgaccaggt
     1501 tctcagccag cgccagttga gcgatgccga gcagcgttac cgcagcatct tcgaggattc
     1561 cgtggtcggc atgttccaga cctcgatcga cggccgctac atcgccgcca accgcgcgct
     1621 ggcgcagctt tatggttacg ccggggcggc cgaactgatc gccggtctgt ccgacatcgc
     1681 cggcgatctc tatgtcgaat cagggcgtcg cgacgagttc gagacgctga tgcgcgagca
     1741 cggccgcgtc agcgatttcg aatccgaggt ctatcggcgc gacggcagtc gcatctggat
     1801 ttccgagcac gcccatgtcg tccgcggtcc cggcggcgag ccgctgtatt acgagggtac
     1861 ggtcgaggac atcaccgccc agtaccacta tcgccagcaa ctggagtacc aggcgacgca
     1921 tgatccgctg accggcctgc ccaaccgcaa cctgttgcag gaccgcctgc agcaggtggt
     1981 gcgtctggcc agccggcgtg gcaccaaggg cagcctggcc tttgtcgacg tcgataactt
     2041 caagctggtc aacgacagtc tcggccatgg cgccggcgac aagctgctgg tcgaagtcgc
     2101 ccgccgtctc aaatcctgct tgcgcgattc ggataccgtt gcccgctacg gcggcgacga
     2161 gttcgttctc atcctcggcg actacgacgg tctcgccgag accttgcaca tcctgcaccg
     2221 catccaggac gccatcgccg agccgatcac cctggacggc caggaggtcc gcgtcaattg
     2281 cagcatcggc gtcagcatct tccccgacga cggcgtcgac ctcgatgtcc tgctgcgcca
     2341 cgccgacgcc gcgatgcacc atgccaagaa gctgggcaag gggcagttcc agttctacac
     2401 cgaaaccctc aacgtcgagg cccgcgaacg cctagcgctg gattcggcct tgcgccgggc
     2461 gatcgaggag gaggcgctga gcgtcgttta tcagccgaag gtcaatgccc gcggtcgcgc
     2521 ctgcggcttc gaggcactgg tgcgctggga gagcgccgaa ttcggcagcg tttcgccggt
     2581 acgtttcatt ccgctggccg aagaaaatgg cctgatcggc cagctcacct ggttcgtcct
     2641 gcgccaggcc tgccatcagg ccgccgcctg gcgtgccgcc ggccacccgg acctgcgcat
     2701 cgccgtcaat atttcgccca gcctgttccg cgagaaggat cttgccgcgc tggtcggcgc
     2761 cgtccttgag gaagccgggc tgccggctgc caacctcgag ctggagatta ccgagggcat
     2821 gctgatgggc gacgttcagc gcagcctggc ggtgctcggc gaactgaagg cgatgggcgt
     2881 cgatatcgcg gtggatgact tcggtaccgg ctattcctcg ctcgcttacc tgaagcgttt
     2941 cccgatcgac attctcaagg tggaccgctc cttcgtcctc gaatgcgaca gcggcaacgg
     3001 cggtcttgcc atcacccgcg cc
//
LOCUS       DAOSDX010000295         3011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11534_length_3011_cov_3.011164, whole genome shotgun sequence.
ACCESSION   DAOSDX010000295 DAOSDX010000000
VERSION     DAOSDX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11534_length_3011_cov_3.011164"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLN02_07460"
     CDS             complement(<1..207)
                     /locus_tag="PLN02_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52200.1"
                     /translation="MIVASGQAKGFVATPELAGEAVARALERASLRRAEFVLLLLTRD
                     FTRLLPETLRCAASAAACLQISGCT"
     gene            complement(204..836)
                     /gene="nth"
                     /locus_tag="PLN02_07465"
     CDS             complement(204..836)
                     /gene="nth"
                     /locus_tag="PLN02_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOI52201.1"
                     /translation="MKKSVVEQFYQRLRENNPAPTTELAYATPFQLLIAVILSAQATD
                     VGVNKATARLFPVAPDAASMLALGEDGLRDYIKTIGLYRTKAKNVIATCRILLERHAG
                     EVPADRAALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRLGNRTGLAPGKTVVEVE
                     NKLLRVTPREFLKDAHHWLILHGRYVCKARQPACAACCVYDLCEFRDKTA"
     gene            1072..2085
                     /locus_tag="PLN02_07470"
     CDS             1072..2085
                     /locus_tag="PLN02_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOI52202.1"
                     /translation="MKKLLASLLLLVVVVGAAWYSMTGRDDDNLLDEDFTLPNDGATL
                     ALRPLPLPKQQPPLRVSLGERLFFDTRLSADKTIASATCHRFDLGGADGQQVSIGIGG
                     ARGPINAPTVFNSGYAFAQFWDGRAATLEEQAAGPVHNPLEMGSSWPVVLERLNADAA
                     IRRDFSLAYADGLTAANVANAIATFERTLITQDAPFDRYLAGDRQAIGKLAIDGYQRF
                     RDYGCISCHQGVLLGGNMYQKFGVLGDYFADRKLTTADLGRYNVTGREEDKHVFKVPS
                     LRNVALTAPYFHDGSTYSLAAAVAIMGRYQLGRELSQEEVAAIVAFLESLTGKQPVKG
                     LTQ"
     gene            2082..>3011
                     /locus_tag="PLN02_07475"
     CDS             2082..>3011
                     /locus_tag="PLN02_07475"
                     /inference="COORDINATES: protein motif:HMM:NF039312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52203.1"
                     /translation="MSLTLAELRAHRGWKVMLVVTLTTLLTWLFFKADEVSSQAHDNY
                     TRMLRQFQRYDEQLASKVVASHAGLVQNYDGMAFYLARLRETGQSLRSFPAWLNGTQS
                     ESIARTLQELIAAEEARALDIDRFQRANAVLRNSKLYLPQAANALLVSESWPTAGAFG
                     DFIRHLVGISMTRNPDDSELRQLRRELQALRNAGGPPALAGILVHAEVLLEQSPVVAT
                     LVDRITRAPTAELHDKLLRSYLEAYRSAVASASYHRTLLYAIAMMLLGYALYAVFRIE
                     RDRRALRLAHADLATRYEAQRRAEGELRLYGTVF"
BASE COUNT          507 a          917 c         1023 g          564 t
ORIGIN      
        1 cgtacaaccg ctgatctgca ggcaggccgc ggcgctggcg gcgcagcgca gggtttcggg
       61 caagaggcgg gtgaaatcgc gcgtcagcag cagcaagacg aattcggcgc ggcgcaggct
      121 tgcccgctcg agggcgcgcg cgaccgcctc gccggccagc tcgggcgttg cgacaaaccc
      181 tttcgcctgg ccgctggcga cgatcacgcg gtcttgtccc tgaattcgca gagatcgtag
      241 acgcagcacg cggcgcaggc cggttggcgt gccttgcaga cgtagcggcc gtgcaggatc
      301 agccagtgat gcgcatcctt gagaaattcg cgcggggtga cgcggagcag cttgttttcc
      361 acttcgacga cggtcttgcc gggggccagg ccggtgcggt tgccgagacg gaagatgtgc
      421 gtgtcgacgg cgatcgtcgg ttgaccgaaa gccgtgttga gaacgacgtt ggcggtcttg
      481 cggccgacac cgggcagtgc ttcgagcgcg gcgcggtcgg cgggaacctc gccggcgtgt
      541 cgttcgagca ggatgcggca ggtggcaatg acgtttttcg ccttggtccg gtagaggccg
      601 atcgtcttga tgtaatcgcg caggccatct tcgccgaggg cgagcatcga ggcggcgtcg
      661 ggggcgaccg ggaagaggcg ggcggttgcc ttgttgacgc cgacatcggt cgcctgggcg
      721 gagaggatga cggcgatcag gagttggaag ggggtcgcgt aagccaactc ggtggttggc
      781 gccggattgt tttcgcgcag gcgctgatag aactgttcga cgacggattt tttcacggtc
      841 aggcgggggc ggggattgtc agtcgttagg gaatgcgcga tattggcggg cgatgcattt
      901 taactgatga agaatagagc aaacgacgca gatgcgctta cggtcgagga ggaggcaggc
      961 atcgggagat tccgttgtcg ccgtgccgga tgattttcgc gaggcgttgc gggttgctga
     1021 tgtgcgggcg gtgggtgtga acggggaagc ccaccatctc gcggggccgc catgaagaaa
     1081 ctgctggctt ccctgttgct gctggtcgtg gtggtcggtg cggcctggta ttcgatgacc
     1141 gggcgcgacg atgacaacct gctggacgaa gacttcacgc tgcccaacga tggcgcgacg
     1201 ctggccctgc gtcccctgcc tctgccaaaa cagcagccgc cgctgcgcgt ttcgctcggc
     1261 gagcgcctgt tcttcgatac ccgcctctcc gccgacaaga cgatagcctc cgccacctgc
     1321 catcgcttcg atctcggggg cgccgacggc cagcaggttt cgatcggcat cggcggcgcc
     1381 cgtggtccga tcaatgcgcc gaccgtcttc aacagtggct atgccttcgc ccagttctgg
     1441 gacgggcggg cggccaccct tgaggagcag gcggccgggc cggtgcacaa tccccttgaa
     1501 atgggatcgt cgtggccggt cgttctcgaa cgtctgaatg ccgatgcggc gatccggcgg
     1561 gatttttcgc tggcctatgc ggacggtctg acggcggcca atgtcgccaa cgcgatcgcc
     1621 accttcgaac gcaccctgat cacccaggac gcgcccttcg atcgctatct ggcgggagat
     1681 aggcaggcca tcggcaaact cgccatcgac ggttatcagc gtttccgcga ctatggctgc
     1741 atcagttgtc accagggggt gctgctgggc ggcaacatgt atcagaaatt cggcgtcctc
     1801 ggcgactact ttgccgatcg caagctgacc acggccgatc tcgggcgcta caacgtcacc
     1861 ggtcgcgagg aggacaagca tgtcttcaag gtaccgagtc tgcgcaacgt tgccctgact
     1921 gcgccctatt tccatgacgg ttcgacctat tccctggccg ccgcggtcgc catcatgggg
     1981 cgctaccaac tggggcgcga gttgtcgcag gaagaagtgg cggcgatcgt cgcctttctc
     2041 gaaagcctga ccggcaagca gcccgtcaag gggctgaccc aatgagcctg actctggccg
     2101 aactgcgggc gcaccgggga tggaaggtga tgctggtcgt caccctgacc accttgctga
     2161 cctggctgtt tttcaaggcc gacgaagtct cgtcgcaggc gcacgataac tacacccgca
     2221 tgctgcgcca gtttcagcgc tacgacgaac aactggcttc caaggtcgtc gccagccatg
     2281 ccgggctggt ccagaactac gacgggatgg ccttctatct ggcccgcctg cgggaaaccg
     2341 gccagtcgct gcgatccttt cccgcctggc tgaacgggac gcaaagcgaa tcgatcgccc
     2401 ggacgttaca agaattgatc gccgccgaag aggccagggc gctcgatatc gaccgctttc
     2461 agcgggccaa cgccgtcctg cgtaattcca agctgtattt gccgcaggcg gcaaatgccc
     2521 tgcttgtcag cgaatcgtgg ccaaccgcgg gggcttttgg cgatttcatc cgccacctgg
     2581 tcggcatcag catgacgcgc aacccggatg acagcgaatt gcgccagttg cgccgggaac
     2641 tgcaggcgct gcgcaatgcc ggggggccgc cagcgctggc cggtattctg gtgcatgccg
     2701 aagttctgct cgaacagagt ccggtggtgg caaccctggt cgaccggatc accagggcgc
     2761 cgaccgccga actccatgac aagctgttgc gcagttacct ggaggcttac cggtcggcgg
     2821 tcgcttccgc ttcctatcac cgcacgctgc tctacgcgat cgccatgatg ctgctcggct
     2881 acgcgctgta cgcggtgttc cgcatcgagc gtgaccggcg cgccttgcgc ctcgcccacg
     2941 ccgacctggc cacgcgctac gaggcgcagc ggcgggccga aggcgagttg cgcttgtatg
     3001 gcacggtgtt t
//
LOCUS       DAOSDX010000296         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11536_length_3010_cov_2.872420, whole genome shotgun sequence.
ACCESSION   DAOSDX010000296 DAOSDX010000000
VERSION     DAOSDX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11536_length_3010_cov_2.872420"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PLN02_07480"
     CDS             <1..1206
                     /locus_tag="PLN02_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOI52204.1"
                     /translation="SLISTVLATQFPGPGTILVDQALHFARPVTIGDRIVITVTVKHK
                     FDHNRHILFDCLCTNQEGLPVVKGTAEVLAPSEKMSRLQETQAPEIYIDDKHARYTQL
                     LSRVKGLEPIPTAVAHPCDQESLRGPVIAFQAGIIEPILVGPEAKIRAVAEEFAIDLS
                     GIRIVNAAHSHDSAALAVSLIKTGDAEALMKGSLHTDELMSEVVARANGLRTNRRISH
                     VFVMDVPTYHRPLLITDAAINIAPTLEEKVDIIQNAIDLAHVLDIPEPKVAILSAVET
                     VNPKIQSTLDAAALCKMADRGQIKGGLLDGPLAFDNAVSIVAAKTKGIKSAVAGHAEI
                     LVVPDLESGNMVAKQLEYLANALTAGIVLGTKVPIVLTSRADTAETRTASCVIAALVA
                     HAKRAKENP"
     gene            1212..2393
                     /locus_tag="PLN02_07485"
     CDS             1212..2393
                     /locus_tag="PLN02_07485"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOI52205.1"
                     /translation="MQQGILTINAGSSSIKFALFPLAHPISPQAEVCGQIDGIGTNAT
                     KLVAHDRAGERIANQAIAGDAVSHSAALDAMLQWFLATYTGWQIVAVGHRVVHGGERY
                     SKPIIVDTTVLGHLESFIPLAPLHEPHNVAGIKALQALLPEVPQIACFDTAFHRSQPD
                     VAQLFGLPRALTAEGIKRYGFHGLSYEYIARALPQHSHRANGRVVVAHLGNGASMAAM
                     VNRKCVATTLGFSTIDGLMMGTRCGNLDPGLILHLMETKGFTVKDMTRILYKESGLLG
                     VSGISQDMRTLLASSKPEAHEAVELFCYRIARELGSLAAAAGGLDALVFTGGIGEHAA
                     EVRRRVCLQSEWLGIRVNPEANARHDIVISAGNSSVDVLVVPTNEEWMMAHHAQTLLA
                     L"
     gene            2593..2775
                     /locus_tag="PLN02_07490"
                     /pseudo
     CDS             2593..2775
                     /locus_tag="PLN02_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2911..2991)
                     /locus_tag="PLN02_07495"
                     /pseudo
     CDS             complement(2911..2991)
                     /locus_tag="PLN02_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
BASE COUNT          569 a         1086 c          908 g          447 t
ORIGIN      
        1 tcgctgattt cgaccgtgct ggccacccag ttccccggcc cgggcacgat cctggtcgac
       61 caggcgctgc attttgcgcg gccggtaacg atcggcgacc gcatcgtcat caccgtcacc
      121 gtcaagcaca agttcgacca taaccggcac atcctcttcg actgcctgtg caccaaccag
      181 gaaggcctgc cggtggtcaa gggcacggcg gaggtgctgg cgccgagcga gaagatgtcg
      241 cgcctgcagg aaacacaagc gccggaaatc tacatcgacg acaagcacgc ccgctacacc
      301 cagctcctgt cgcgggtgaa ggggctggaa ccgatcccga cagcggtcgc ccacccctgc
      361 gaccaggagt cgctgcgcgg gccggtgatc gccttccagg ccggcatcat cgaacccatc
      421 ctggtcggtc cggaagccaa gatccgcgcc gttgccgagg aattcgccat cgacctgtcc
      481 ggcatccgca tcgtcaatgc cgcgcacagc cacgattcgg cggcgctcgc cgtgtcgctg
      541 atcaagaccg gcgacgccga ggcgctgatg aagggcagcc tgcacaccga cgaactgatg
      601 agcgaggtgg tcgcccgcgc caacggcctg cgcaccaacc gccgcatcag ccacgtcttc
      661 gtcatggacg tgccgaccta ccaccggccg ctgctgatca ccgacgcggc gatcaacatt
      721 gcgccgacgc tggaggaaaa ggtcgacatc atccagaacg cgatcgacct cgcccacgtc
      781 ctcgacatcc cggaacccaa ggtggcgatc ctgtcggcgg tcgaaacggt caacccgaag
      841 atccagtcca cgctcgacgc cgccgccctg tgcaagatgg ccgaccgcgg gcagatcaag
      901 ggcggcctgc tggacggtcc gctcgccttc gacaacgcgg tgtcgatcgt cgccgccaag
      961 accaagggca tcaagtcggc ggttgccggc catgccgaaa tcctcgttgt ccccgatctc
     1021 gaatccggca acatggtggc caagcagctc gaatacctgg ccaacgcgct gaccgccggc
     1081 atcgtgctcg gcaccaaggt tcccatcgtc ctgaccagcc gcgccgacac cgcggagacg
     1141 cgtaccgcgt cctgcgtcat cgccgccctg gtcgcccacg ccaaacgcgc caaggaaaat
     1201 ccatgagcca aatgcagcaa ggcatcctga ccatcaacgc cggatcgtcg tcgatcaagt
     1261 tcgcgctgtt cccgctcgcc catccgattt cgccgcaggc cgaagtctgc ggtcagatcg
     1321 acggcatcgg caccaacgcc accaagctgg tcgcccacga ccgcgccggc gaacgcatcg
     1381 ccaaccaggc catcgccggc gacgcggtca gccacagcgc ggcgctcgac gccatgctgc
     1441 agtggttcct cgccacctac accggctggc agatcgtcgc cgtcggccac cgcgtcgtcc
     1501 acggcggcga gcgctactcg aagccgatca tcgtcgacac caccgtgctc ggccacctgg
     1561 aaagcttcat cccgctggcg ccgctgcacg aaccgcacaa cgtcgccggg atcaaggcgc
     1621 tgcaggcgct gttgccggaa gtgccgcaga tcgcctgttt cgacaccgcg ttccaccgca
     1681 gccagccaga tgtcgcccag ctcttcggcc tgccgcgcgc gctgaccgcc gagggcatca
     1741 agcgctacgg cttccacggc ctctcctacg aatacatcgc ccgcgccttg ccgcagcatt
     1801 cgcaccgcgc caacggccgt gtcgtcgtcg cccacctcgg caacggcgcc agcatggcgg
     1861 cgatggtcaa tcgcaaatgc gtcgcgacga cgctcggctt ctcgaccatc gacggcctga
     1921 tgatgggtac ccgctgcggc aacctcgacc ccggcctgat cctccacctg atggagacca
     1981 agggcttcac ggtcaaggac atgacccgca tcctgtacaa ggaatccggc ctgctcggcg
     2041 tctccggcat cagccaggac atgcgcaccc tgctcgccag cagcaagccg gaagcgcacg
     2101 aagcggtgga actgttctgc taccgcatcg cccgcgaact cggttcgctc gccgccgccg
     2161 ccggcggcct cgacgcgctg gtattcaccg gcggcatcgg cgagcacgcc gccgaagtcc
     2221 gccgccgcgt ctgcctgcag tcggaatggc tgggcatccg cgtcaacccg gaagccaacg
     2281 cccgtcacga catcgtgatc agcgccggca acagcagcgt cgatgtgctg gtcgtgccga
     2341 ccaacgagga atggatgatg gcgcaccacg cgcagacgct gctcgccctc tgaaaataaa
     2401 tttttacgcc ttgggcgccg accgtgaaag cggtcggcgc cttgtttttt cgggcttttt
     2461 ccggcgatga cggcagcacc ccgaggcgat gcagaaaaca ccgcatctag tcaacgtatt
     2521 gcccgcatac actatatcta gtagattacc gggcattcca aaccagataa cacctaccga
     2581 gaggagcttt caatgagcaa aatcgccgag cagttgtcgt tgaccgacat tcctgcaccg
     2641 ccgcaattcg cgccgctgcc cgagcaggaa atctccggcg aggtgctcat cgaaaagtac
     2701 gccaagggca aggaaaccaa cgtccacgac gtgcgcaaac gcgttgcctg ggcgctcgcc
     2761 caggtcgagc aggaaggcga ccacgcgctg gtcggcgccg agcatgcgcc ggtcgatgac
     2821 ggcgaggcgg cgcgcggcgg cgaggtcgag gagcggcggc tcggcggcca tgccgcgcgc
     2881 ctggtcgccg agcaggaccg gcgcctggta gatcagcagt tcgtcgacgc ggccggcggc
     2941 gagcagggcg ccgttgaggc cggcgccgcc ctcgacgagg acttcgttga tgccgcggca
     3001 gccgagctcc
//
LOCUS       DAOSDX010000297         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11537_length_3010_cov_2.068359, whole genome shotgun sequence.
ACCESSION   DAOSDX010000297 DAOSDX010000000
VERSION     DAOSDX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11537_length_3010_cov_2.068359"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLN02_07500"
     CDS             complement(<1..537)
                     /locus_tag="PLN02_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOI52206.1"
                     /translation="MSQSHYSFPDASGHFGPYGGVFVAETLFGALDELKAAYAAAQAD
                     PAFRAEYDYELKHFVGRPSPIYHAKRLSEVCGGAQIYLKREDLNHTGAHKVNNCIGQA
                     LLAKRMGKPRVIAETGAGQHGVATATVAARYGFECVVYMGSEDVRRQAANVYRMKLLG
                     ATVVPVESGSKTLKDALNE"
     gene            complement(661..1296)
                     /locus_tag="PLN02_07505"
     CDS             complement(661..1296)
                     /locus_tag="PLN02_07505"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOI52207.1"
                     /translation="MKTRIKICGLTREADVDAAVAAGADAIGFVFYPPSPRYVTPERA
                     AELACRVPPFVDVVGLFVNATADDVATTSATVPLNLLQFHGDESPEFCRSFARPWLRA
                     ARVRPGLDLVEFARSYSNARGLLLDAFVEGYGGGGHVFDWMLIPPDLPGYLVLSGGLH
                     AGNVGDAIRRVRPVAVDVSSGVEAGKGIKDHDKIAAFVAAVRAADAAVGSI"
     gene            complement(1293..2081)
                     /gene="truA"
                     /locus_tag="PLN02_07510"
     CDS             complement(1293..2081)
                     /gene="truA"
                     /locus_tag="PLN02_07510"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOI52208.1"
                     /translation="MRLALGVEYFGSPFRGWQSQAGGRTVQDALEAALARIAGQPVAT
                     LCAGRTDAGVHAIQQVVHFDAPVERPLTAWVRGVNTHLPDGVAVRWAQPVSDEFHARF
                     SAHGRRYRYLLLNRPQRPGLWQGRVGWFHLPLDLAAMQDACARLPGEHDFSAFRAAQC
                     QAKSPVKLMTAASVRQCGAMFVFDFAASAFLHHMVRNLVGSLVHIGKGARSPGWIDEL
                     LQMRDRKLAAPTFSPDGLYFRGPVYEPLWGLPEPADDFLDGMLT"
     gene            complement(2078..2668)
                     /locus_tag="PLN02_07515"
     CDS             complement(2078..2668)
                     /locus_tag="PLN02_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52209.1"
                     /translation="MHPTAALILWSSAVIAVQYLAYPPLAAFAVLLGVLPGVLRVWLG
                     FVYRARWLLLTLWLILAYNTPGEAYADLVWAPTYEGIAEASLHAARLLVVLGCLSWLL
                     VRLGRDGMLAGLWGALAPARACGLDSERLLVRLALVLERLQEPPEKGAWRRLLHTPPD
                     FAAGSAVVRLEYPAWRARDAWPVLLALLLLLGVALS"
     gene            complement(2731..>3010)
                     /locus_tag="PLN02_07520"
     CDS             complement(2731..>3010)
                     /locus_tag="PLN02_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOI52210.1"
                     /translation="VFLGQPMMNSEFDMDAINLDLSVPPPAPAPDAAAEPERDARWEE
                     VNTKLDLAKAYEEMGDLEGARELLQEVLGEGSADLVAQAQEILGRIGG"
BASE COUNT          560 a         1010 c         1009 g          431 t
ORIGIN      
        1 ttcgttgagc gcgtccttga gcgtcttcga gccggactcg accggcacca cggtggcgcc
       61 gagcagcttc atgcgataga cgttggcggc ctggcgacgc acgtcctcgc tgcccatgta
      121 gacgacgcac tcgaagccat agcgcgcggc gaccgtggcg gtagcgacgc cgtgctggcc
      181 ggcgccggtc tcggcgatga cgcgcggctt gcccatgcgc ttggcgagca gggcctggcc
      241 gatgcagttg ttcaccttgt gcgcgccggt gtggttgagg tcctcgcgct tgaggtagat
      301 ctgggcgccg ccgcagactt cggacaggcg tttggcgtgg tagatcggcg acggacggcc
      361 gacgaaatgc ttgagttcgt agtcgtactc ggcgcggaag gcgggatcag cctgggcggc
      421 agcataagcc gccttcagct cgtcgagcgc accgaacagc gtttcggcga cgaagacgcc
      481 gccatagggg ccgaaatggc cgctggcgtc gggaaaactg taatgcgatt gggacatggc
      541 gaaactcctg gaaaatgggt gaacggaaaa aggcaaaccg cccgtttcag ggatggttcg
      601 cctgatgacg ccgccaacag ttccgggtga tcccacccgc cgatttccgc atcttctttc
      661 ctaaattgag cccaccgccg catcggccgc ccgcacggcg gcgacgaacg cggcgatctt
      721 gtcgtgatcc ttgatgccct tgcccgcttc gacgcccgac gaaacatcga ccgccaccgg
      781 ccgcacccgg cggatggcgt cgccgacgtt gccggcatgc agcccgcccg acaacaccag
      841 ataccccggc aagtccggcg gaatcagcat ccagtcgaag acatgaccgc cgccgccgta
      901 gccttcgacg aaagcatcta gcaacaaacc gcgcgcattg gaatacgagc gcgcgaattc
      961 taccagatcg agccccggcc tcacgcgcgc cgctcgtagc cagggtctgg cgaagctgcg
     1021 acagaactcc ggcgactcgt cgccatggaa ttgcagcagg ttgagcggta ccgtcgcgct
     1081 ggtcgtcgcg acatcgtccg ccgtggcgtt gacgaaaagg ccgacgacgt cgacgaaggg
     1141 cggcacccgg catgccagtt cggcggcgcg ttccggcgtc acgtaacgcg ggctgggcgg
     1201 gtagaagaca aagccgatgg cgtcggcacc ggcggcgacg gcggcatcga catcagcttc
     1261 gcgggtcagg ccgcagatct tgatgcgggt tttcatgtca gcattccatc gagaaaatca
     1321 tcggccggct cgggcaggcc ccagagcggc tcgtagacgg gtccgcgaaa gtaaagccca
     1381 tcgggcgaaa aggtcggcgc cgccagcttg cggtcgcgca tctgcagcag ttcgtcgatc
     1441 cagccgggcg accgggcgcc cttgccgata tggaccagac tgccgaccag gttgcgcacc
     1501 atgtgatgca ggaaggcgct ggccgcgaaa tcgaagacga acatcgcccc gcactggcgt
     1561 accgaggccg ccgtcatcag tttcaccggc gatttcgcct ggcactgcgc cgcccggaag
     1621 gccgagaaat cgtgctcacc gggcaagcgg gcgcaagcgt cctgcatcgc cgccaggtcg
     1681 agcggcaggt ggaaccagcc gacccgtccc tgccagagcc ccggccgctg cggccggttg
     1741 agcagcaggt agcggtagcg tcggccatgc gcactgaagc gggcatggaa ttcatcgctc
     1801 accggctgcg cccagcgcac cgcgacgccg tccggcagat gcgtatttac gccgcgcacc
     1861 caggccgtga gcggccgctc gaccggcgcg tcgaaatgca ccacctgctg gatggcatgg
     1921 acgccggcat cggtacgacc ggcgcacaaa gtcgccaccg gctgcccggc gattctcgcc
     1981 agcgccgcct ccagcgcgtc ctggacggtg cggccgccgg cctggctctg ccagccgcgg
     2041 aagggcgagc cgaaatactc gacgccgagc gccagcctca tgacagcgcc acgcccagca
     2101 acaggaggag ggccagtagc acgggccagg catcgcgcgc ccgccaggcc gggtattcca
     2161 gacggacgac ggcgctgccc gcggcgaaat ccggcggcgt atgcagcagg cggcgccagg
     2221 cgcctttttc cggcggctcc tgcaggcgct cgagcaccag cgccaagcgt accagcagac
     2281 gctcgctgtc cagcccgcaa gcccgcgccg gcgccagggc accccacaaa ccggcgagca
     2341 tgccgtcgcg cccgaggcgg acgagcagcc aggacaggca gccgaggacg acgagcaggc
     2401 gtgccgcgtg caggctggct tcggcgatgc cttcgtaggt cggcgcccag accaggtcgg
     2461 cgtaggcttc cccaggcgtg ttgtaggcca ggatcagcca cagcgtcagc aacagccagc
     2521 gcgcccgata gacgaagccg agccagacgc gcagcacgcc cggcagcacg ccgagcagga
     2581 cggcgaatgc cgccagcggc ggataagcca ggtattgcac ggcgatcacg gcggacgacc
     2641 agaggatcag ggcggcagtg ggatgcaaaa cagctccatc aaaaacagac ggccccagcg
     2701 gggccgtctg ccaagtgcgg gagagtaatt ttacccgcca atgcggccga ggatttcctg
     2761 cgcctgagcc accaggtcgg cagagccttc gccgagaact tcctgaagca attcgcgggc
     2821 cccctcgagg tcgcccattt cctcgtaggc tttggccaga tcaagcttgg tgttgacctc
     2881 ttcccagcgg gcgtcccttt ccggctcggc cgcggcgtcc ggagcaggcg ctggaggcgg
     2941 cacggaaaga tcaagattga tcgcgtccat gtcgaattcc gaattcatca tcggctgacc
     3001 gaggaagacg
//
LOCUS       DAOSDX010000298         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11603_length_2995_cov_3.678571, whole genome shotgun sequence.
ACCESSION   DAOSDX010000298 DAOSDX010000000
VERSION     DAOSDX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11603_length_2995_cov_3.678571"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1245
                     /locus_tag="PLN02_07525"
     CDS             313..1245
                     /locus_tag="PLN02_07525"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOI52211.1"
                     /translation="MTAPLFASSITSLPLLSKGKVRDIYAVDADKLLIVTTDRLSAFD
                     VILPDPIPKKGQVLQAVANFWFAKLGHIVPNQLTGIDPESVVAENEREQVRGRAVVVK
                     RLKPLPIEAVVRGYVIGSGWKDYQATGAICGIALPTGLKLAQKLPAPIFTPATKAEVG
                     DHDENVSFEQAAANCDATLNAALAGTGKTGAQLCGEARDAAIRLYQEASEYAATRGII
                     IADTKFEFGIDAAGTLHLIDEALTPDSSRFWPADQYAEGSNPPSYDKQYVRDYLETLD
                     WNKKAPGPALPAEVVARTSAKYVEAFEKLTGQTL"
     gene            1320..2093
                     /locus_tag="PLN02_07530"
     CDS             1320..2093
                     /locus_tag="PLN02_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOI52212.1"
                     /translation="MHAPSSFHSFGHDLPAIRRVDARAPSRWLEAGWQDIRRTPIASL
                     AYGLLFALGGDLIILALLEHPHLLALSISGFFFVAPLLAAGLYELSRASGNGEKPIFV
                     ESLRVFRHNFQSLALFGLLLALIATLWERFSAISFALLGATSANSASVYLNELLFSGE
                     HLLFSLTWLGLGALLAAFVFALTVVSVPMMVDRECDPVTAMATSLRAVVANPGPLALW
                     ALLIVVLTLTGFATLLFGLVVLMPMLGHASWHAYRELVE"
     gene            2157..>2995
                     /locus_tag="PLN02_07535"
     CDS             2157..>2995
                     /locus_tag="PLN02_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOI52213.1"
                     /translation="MTATNPLLDFTDLPRFDLIQPEHVKPAITQLLADGRALVERLTA
                     DSTPATWADFAGALSDGLEGFGRAWGVVGHLHSVNDVPAWREAYNEMLPEVSRFYTEL
                     GQNLKLFDKYRALKQSPEYATLSAERRKIVDNEIRDFRLGGAELPEDQKPRFQAIMEE
                     LSQLSAKFSENILDATNAFAEIVTDETLLAGLPDYAKDAAREAAEKAGVEGWKFTLHA
                     PSYGPLMQYADNRELRARMYRAYATRAAEIDNGYSKPEWDNGPIMKRLLELKAEDAAM
                     LGF"
BASE COUNT          527 a         1101 c          889 g          478 t
ORIGIN      
        1 cgagacgctc gacgagaaag tcgcgcagcg gctggtacat ctgcaggatg acgcgtgcgc
       61 tcgaatgcgg cgcgacgccg atgcgcagca cggcgggggc tgcccgggcc aggcgcaagg
      121 ccaatggcgc cgccagcatg ccgagcagca acgatcgtcg gttggtcatc gccccctccc
      181 ggcgcgtatt gccaattaat caaaaatgcc aaaaacgaat gccatgatac cgcagactgc
      241 ccccgccggc actggcggca gcgctccggg gcagtggttt ataatccgcc ttttgctttc
      301 gggaccacga ccgtgaccgc tccgctcttc gcctcctcca tcaccagcct gccgctgctc
      361 tccaagggca aggtccgcga catctacgcg gtcgacgccg acaagctgct gatcgtcacc
      421 accgaccgcc tgtcggcctt cgacgtcatc ctgcccgatc cgatcccgaa gaagggccag
      481 gtcctgcagg cggtggcgaa tttctggttt gccaagctcg gccacatcgt ccccaatcag
      541 ttgaccggca tcgatccgga atcggtcgtt gccgaaaatg aacgcgagca ggtgcgcggc
      601 cgcgccgtcg tcgtcaagcg cctgaagccg ctgccgatcg aggccgtcgt gcgcggctac
      661 gtgatcggct ccggctggaa ggattaccag gcgaccggcg ccatctgcgg catcgccctg
      721 ccgaccggcc tcaagctggc gcagaagctg ccggcgccga tcttcacgcc ggcgaccaag
      781 gccgaagtcg gcgaccacga cgagaacgtc tccttcgaac aggccgccgc caactgcgac
      841 gccacgctga acgccgccct cgccggtacc ggcaagaccg gcgcccaact gtgcggcgaa
      901 gcgcgcgacg ccgccatccg cctctaccag gaagcctccg agtacgcagc aacgcgcggc
      961 atcatcatcg ccgacaccaa gttcgagttc ggcatcgacg ccgccggcac gctgcacctg
     1021 atcgacgagg cgctgacccc ggattcctcg cgtttctggc cggccgacca gtacgccgaa
     1081 ggcagcaacc cgccgtccta cgacaagcag tacgtgcgcg actacctgga aaccctggac
     1141 tggaacaaga aggcaccggg accggcactg ccggcggaag tcgtcgcccg caccagcgcc
     1201 aagtacgtcg aagccttcga gaagctgacc ggccagacgc tgtaaaccgg gcacgggccg
     1261 ggaacctccg gcccttgcca atggtctgaa ggggggtccg catcgctcag gagttcaaca
     1321 tgcacgcccc ttcaagcttc cacagtttcg ggcacgacct gccggcgatt cgccgcgtcg
     1381 atgcccgcgc ccccagccgc tggctggaag ccggctggca ggacatccgc cggacgccga
     1441 tcgcctcgct cgcctacggc ctgctcttcg cgctgggcgg cgacctgatc atccttgccc
     1501 tgctcgaaca cccgcacctg ctggcgctgt cgatttccgg cttcttcttc gtcgcgccgc
     1561 tgctcgccgc cggcctttac gagctgagtc gcgccagcgg caacggcgaa aagccgatct
     1621 tcgtcgaatc gctgcgcgtc ttccgccaca acttccagtc gctggcgctg ttcggcctgc
     1681 tgctggcact gatcgccacg ctctgggaac gtttttcggc gatctccttc gcgctgctcg
     1741 gcgccacctc ggccaactcc gccagcgtct atctcaacga actgctgttc tccggcgaac
     1801 acctgctctt cagcctgacc tggctcggcc tcggcgcgct gctcgccgcc ttcgtcttcg
     1861 cgctgaccgt ggtttcggtg ccgatgatgg tggaccggga atgcgacccg gtgaccgcga
     1921 tggccaccag cctgcgcgcg gtcgtcgcca accccggtcc gctggcgctg tgggcgctgc
     1981 tgatcgtggt cctgacgctg accggcttcg ccaccctgct cttcggcctg gtcgtcctga
     2041 tgccgatgct cggtcacgct tcgtggcacg cctaccgcga gctagtagaa taggacgctt
     2101 gacgccaaac acggggcggc agcgaccgcc cctttttctt gccccgcgaa aagaccatga
     2161 ccgccaccaa tcccctgctc gatttcaccg acctaccccg cttcgacctg atccagccgg
     2221 aacacgtcaa accggcgatc acccagctac tcgccgacgg ccgcgccctg gtcgagcgcc
     2281 tgaccgccga cagcacgccg gccacctggg ccgactttgc cggtgccctc agcgacggcc
     2341 tggaaggttt cggccgcgcc tggggcgtcg tcggtcacct gcattcggtc aacgacgtgc
     2401 cggcctggcg cgaggcctac aacgagatgc tgccggaggt gtcgcgcttc tacaccgagc
     2461 tcggccagaa cctgaagctg ttcgacaagt accgggcgct gaagcagagc ccggaatacg
     2521 ccacgctctc cgccgaacgc cggaagatcg tcgataacga gatccgcgat ttccgtctcg
     2581 gcggcgccga actgccggaa gaccagaaac cgcgcttcca ggcgatcatg gaagagctgt
     2641 cgcagctttc cgccaagttc tcggaaaaca tcctcgacgc gaccaacgcc ttcgccgaaa
     2701 tcgtcaccga cgagacgctg ctcgccggcc tgcccgacta cgccaaggac gccgcccgcg
     2761 aagccgccga aaaagccggt gttgaaggct ggaaattcac cctgcacgcg ccgtcctacg
     2821 gcccgctgat gcagtacgcc gacaaccgcg aactgcgcgc ccgcatgtac cgcgcctacg
     2881 ccacccgcgc cgccgagatc gacaacggct actcgaagcc ggaatgggac aacggcccga
     2941 tcatgaagcg cctgctcgaa ctcaaggccg aggacgcggc catgctcggc ttcaa
//
LOCUS       DAOSDX010000299         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11656_length_2983_cov_3.487022, whole genome shotgun sequence.
ACCESSION   DAOSDX010000299 DAOSDX010000000
VERSION     DAOSDX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11656_length_2983_cov_3.487022"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PLN02_07540"
     CDS             complement(<1..1133)
                     /locus_tag="PLN02_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOI52214.1"
                     /translation="MAFQQGLSGLNSSARALDVISSNVANAATAGFKASSTVFADAFA
                     SAMGGGSTSAQVGMGSVNQAVRQSFTQGAVTATGNSLDMAIGGNGFFVIQRGNDTVAY
                     TRNGQFDLDKNGYVVTALGERLMGYQSVGSDGMVSYIGSTIPRPLYVPPQGIAAKATG
                     NTGAAVTVRANLDSQLEVPATSPFDVNDPDSYSFATSVAVYDSLGNQHSLNMYFVREA
                     PTTSNTWGVYTTFDNSAPTTQSDIVFDSTTGAWESGGEGTISIPSATLGTGAADLTFD
                     YDLSQFTQRRADSAVYETTQDGSPPGEVASVSISADGIMQASYTNGQTRELGRVILAT
                     FRNPNGLSSLGDNLWGETIDSGSPAVAAPGEGLNGTISSGRIE"
     gene            complement(1147..1818)
                     /locus_tag="PLN02_07545"
     CDS             complement(1147..1818)
                     /locus_tag="PLN02_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HOI52215.1"
                     /translation="MSTESVTSSTTHPLYQEKTTTKTTSAADDMQTQFLTLLTTQLKN
                     QDPLNPTDANQMTAQLSQISTVSGIEKLNTTMEKLLSSYSAVQNMQAAAMIGKTVFIA
                     GNEMELGSEGAIAGFNLESAAESVTVKIRDSDGNLVTTQDIGAMSAGIGSFYWDGTKA
                     DGTKAAEGTYNFTFEATKGTTKVKGESLQAGTISAVTMLSSGAALVLNGNKSVSYTDI
                     RQILN"
     gene            complement(1831..2247)
                     /gene="flgC"
                     /locus_tag="PLN02_07550"
     CDS             complement(1831..2247)
                     /gene="flgC"
                     /locus_tag="PLN02_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOI52216.1"
                     /translation="MSLFNIFRVSSSAMTAQSMRLNTVASNLANADSIVTSDGKAYRS
                     KQVVFEATPMGGDASVSTGVRVRQVVESAAPARMVYDPKNPAADAKGYVSYSNVNVVE
                     EMTNMISASRSYQANVEVMNTAKSLMMRTLQIGQGQ"
     gene            complement(2262..2672)
                     /gene="flgB"
                     /locus_tag="PLN02_07555"
     CDS             complement(2262..2672)
                     /gene="flgB"
                     /locus_tag="PLN02_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOI52217.1"
                     /translation="MANLESHLGVYSKALDLRAQRQQVLASNIANADTPNYKARDFDF
                     SQAMAQAMAGRPSDGALPLARTSAGHLGAGNGAGSVRLQYRGEAQSAVDGNTVDMDVE
                     RAQIADNAIQYQILTQLISDKFSGLRTAMSGTQG"
     gene            2766..>2983
                     /locus_tag="PLN02_07560"
     CDS             2766..>2983
                     /locus_tag="PLN02_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HOI52218.1"
                     /translation="MPAFLLLTLLLLVTPPLAASEPQAILKAAEDLARKLATGQPGEV
                     QVVAGPIDSSQLPACSALETSPPATPRNL"
BASE COUNT          531 a          945 c          888 g          619 t
ORIGIN      
        1 tcctcgatcc gtcccgagct gatcgtgccg ttcaggccct cacccggcgc ggcgactgcc
       61 ggactgccgg aatcgatcgt ctcgccccag aggttgtcgc ccagcgagga aagaccgttc
      121 ggattacgga aggtggcaag gatgacacga ccgagttcgc gcgtctgacc attggtgtag
      181 ctcgcctgca tgatgccgtc ggccgagatc gaaacactgg ccacttctcc cggcggcgaa
      241 ccgtcctgag tcgtttcata aaccgcggaa tcggcacgcc gctgcgtgaa ttgcgacagg
      301 tcgtaatcga aggtcaggtc ggcagcaccg gtacccagag tggccgaggg aatgctgatc
      361 gtcccttccc cacccgactc ccaggcgccc gtcgtcgagt cgaagacgat gtcgctctgc
      421 gtggtcggcg cgctgttgtc gaaggtggtg taaacccccc aggtattcga cgtcgtcggc
      481 gcctcgcgca cgaaatacat gttcagggaa tgctggttgc caaggctgtc gtagaccgca
      541 accgatgttg cgaaactgta agaatccgga tcattgacat cgaatggcga agtcgccggc
      601 acttcgagct gcgagtcgag gttggcgcgc accgtcaccg cggcgccggt attaccggtc
      661 gccttggcgg cgatcccctg cggtggcacg tagagcgggc gcgggatcgt cgaaccgata
      721 taggacacca tgccgtcgga accgacgctc tggtagccca tcaaacgctc gccgagcgcc
      781 gtcacgacgt aaccgttctt gtcgaggtcg aactgcccgt tacgggtgta ggcgaccgtg
      841 tcgttgccgc gctggatgac gaagaagccg ttgccgccga tcgccatgtc cagcgaattg
      901 ccggttgcag tgaccgcgcc ttgcgtgaag ctttgccgga ccgcctgatt gacgctgccc
      961 atgccgacct gcgcggaggt ggaaccaccg cccatggccg atgcgaatgc atccgcgaac
     1021 acggtgctgc ttgccttgaa ccccgccgtc gcggcgttgg ccacgttgct ggagatcacg
     1081 tcaagcgccc gcgccgacga gttcagcccg ctaagtcctt gttgaaatgc catgatcttt
     1141 ctccactcag ttcagtattt gccggatgtc tgtgtagctg acgctcttgt tgccgttgag
     1201 caccagcgca gcaccggagg acagcatggt tacagcacta atcgtgccag cctgcaggga
     1261 ttcgcccttg accttggtcg ttcccttggt cgcttcgaaa gtgaagttgt aggtcccctc
     1321 ggccgccttg gtcccgtccg ccttcgtgcc gtcccagtag aagctgccga tgccggccga
     1381 catggcgccg atgtcctggg tcgtcaccag atttccgtcg ctgtcacgga tcttcacggt
     1441 aacgctttcg gcggcacttt cgaggttgaa gccggcaatc gccccttcgc tgccaagttc
     1501 catttcgttg ccggcaataa ataccgtttt gccgatcatt gctgccgcct gcatgttctg
     1561 gacggcgcta tagctgctca gcagcttttc catcgtcgtg ttcagcttct cgatgccgct
     1621 gaccgtactg atctgcgata gctgcgccgt catctgattg gcatcggtcg gattcagcgg
     1681 gtcctgattc tttagctgcg tcgtcagcag cgtcaggaat tgggtctgca tgtcatcggc
     1741 ggccgaagtc gtcttggtcg tcgtcttttc ctggtacagg ggatgcgtcg tggaagaggt
     1801 tacgctttca gtgctcatgt caaactccgg ttattgcccc tggccgatct gcaaagtccg
     1861 catcatcaga ctcttggcgg tattcatcac ttccacgttg gcctggtagg aacgggaagc
     1921 cgaaatcatg ttggtcattt cctcgacgac attgacattc gaataggaca catagccctt
     1981 ggcgtcagcc gccggattct tcgggtcgta gaccatgcgc gccggggccg cgctttccac
     2041 cacctggcgg acgcggacgc cggtggatac cgaggcgtcg ccacccatcg gcgtcgcttc
     2101 gaaaaccacc tgcttggagc gataggcctt gccgtccgag gtgacgatgc tgtcggcgtt
     2161 ggcgagattt gacgccaccg tgttgaggcg catcgactgc gccgtcatgg cgctcgaaga
     2221 aacccggaag atgttgaaca ggctcatccg tattctcctg cttagccttg agtgccgctc
     2281 atcgcggtac gcaggccgct gaacttgtcg ctgatcaact gggtcagtat ctggtactgg
     2341 atcgcgttgt cggcgatctg cgcgcgctcg acgtccatat cgacggtatt gccatcgacc
     2401 gccgattgcg cctcgccacg gtactgcagg cgcacgctgc cggcgccatt gccagcaccg
     2461 agatgcccgg cggaagtccg cgccagcggc aacgcgccat ccgacggccg gccggccatg
     2521 gcctgcgcca tcgcctggct gaaatcgaag tcgcgcgcct tgtagttcgg cgtatcagcg
     2581 ttggcgatgt tcgaggcgag cacctgctgg cgctgcgccc gcagatcgag cgctttgcta
     2641 tagaccccaa ggtgactttc aagattggcc atctgcctta tctccgaaga tttgcagcaa
     2701 acaaaagcag ataacgtgcc agacaacagc ttcacccgga cacccggctg gggcacaatg
     2761 acgccatgcc tgcattcctg ctcctgaccc tgttgctgct ggtgacacca ccgctcgctg
     2821 ccagcgaacc gcaagcgatc ctgaaagcgg cggaagacct tgcccgcaaa ctggcaaccg
     2881 gccaacccgg cgaagttcag gtggtcgccg gaccaatcga cagcagccag ctacccgcct
     2941 gcagcgcgct ggaaaccagc ccgccggcca ccccgcgcaa cct
//
LOCUS       DAOSDX010000300         2983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11658_length_2983_cov_3.364071, whole genome shotgun sequence.
ACCESSION   DAOSDX010000300 DAOSDX010000000
VERSION     DAOSDX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11658_length_2983_cov_3.364071"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLN02_07565"
     CDS             complement(<1..317)
                     /locus_tag="PLN02_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOI52219.1"
                     /translation="MSEAMTLALLLGLMTVILLQVALLWRGSRPGEEAAALRTLQDGL
                     DKGLARLERELREELARSRREDAEEAFRDREERAQSANLLGQAVSTQMGQLGTLQAER
                     LEA"
     gene            complement(322..>2983)
                     /locus_tag="PLN02_07570"
     CDS             complement(322..>2983)
                     /locus_tag="PLN02_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOI52220.1"
                     /translation="ESELNLQLDGFDLAQLQPYLPAGVPLRIAAARLHSQLRLVFREL
                     TDGANSLTLTGQARLDGLALSDDGGRPLVGWKSLLVDLEQFDPFKRNLALRKVTLDGA
                     ELDLAVDGRGELNLLAVANRLARPAAAAPSATPRATPFAWSLAAFELNDARVRWRDAS
                     GPQPVSGEIGALNARLGAVDHRLAAPLEITEAGWQMDFGTPLKVGSVGLKALRVDLAA
                     QRVEIGELALSGARIAAGLAADGSFAWLTPPVLRGVAQGGKAPAPRPWLAQVAKLKVD
                     DLGLRFEDQSVQPAAVQEISGLSLQGERLGNLPNEKGRLTLQATVNGEGKLAVAGEVQ
                     AVPLAAKLQIDSSGIPVQGLQAYSGRYLNIRLQRGQFSNQGEAVVRVDGGRLKAAYKG
                     SATIGNLLAVDPDNKADFLKWKSLHFGGVDFRLDPLAIDVREIALADFYSRLILNKDG
                     RLNLADIVRRNEAAPVAAADPAAAPVNPPPVAAATTPAAPPVPIRIAKVTLNNGTVNF
                     SDYFVKPNYTVNVGKLNGRVSGLSSAAGTLADMELRGTYAGSAPVLISARLNPLAAQS
                     YLDLKGEVKGVDMTGFSPYSGKYAGYLIDKGKLSLNVAYKLENRQLSADNRLFIDQFT
                     FGERVDSPDATKLPVNLALALLRNNRGEIDINLPISGSLDDPQFSVGGLVIKVIVNLF
                     VKAVTSPFALIGSLFGGGEELSTLAFAPGRTQFDAAAQKKLETLARALSERAALKLEI
                     AGYADPESDSEGVRRVAIERAMQREKFADLQKKGGDAESADAVRIAPEEYKTYLIRVY
                     KAGKFPKPRNFIGLQKDLPVEEMEKLLLANLPAGDEELRLLARQRAENVQTWLIEQGK
                     VPAERIFLVPGKAKAEAPAAAGGRVEFALR"
BASE COUNT          441 a         1048 c         1001 g          493 t
ORIGIN      
        1 aaggcttcca ggcgctccgc ctgcaacgtg ccgagctggc ccatctgggt actcaccgcc
       61 tggccgagca ggttggccga ctgggcgcgt tcctcgcggt cgcggaaggc ctcctcggcg
      121 tcctcgcggc ggctgcgcgc cagttcctcg cgcagctcgc gctcaagccg ggcaagcccc
      181 ttgtccagcc cgtcctgcaa ggtgcgcaag gcggccgcct cctcgcccgg ccggctgccg
      241 cgccagagca gcgcgacctg cagcaggatc accgtcatca ggccgagcag cagggccagc
      301 gtcatcgcct cgctcaccgc cctacctcaa cgcgaattcg acgcgcccgc cggccgccgc
      361 cggcgcctcg gccttcgcct tgccgggcac caggaagatg cgctcggctg gcaccttgcc
      421 ctgctcgatc agccaggtct ggacgttctc ggcgcgctgc cgggcgagca gacgcagttc
      481 ttcgtcgccg gccggcaggt tggcgaggag cagcttttcc atttcctcga ccggcagatc
      541 cttctgcaga ccgatgaagt tgcgtggttt cgggaacttg ccggccttgt acacccggat
      601 caggtaggtc ttgtattcct ccggcgcgat gcgtacggca tcggccgatt cggcgtcgcc
      661 gcccttcttc tgcaggtcgg cgaacttctc gcgctgcatc gcccgctcga tggcgacgcg
      721 ccggacgccc tcgctgtcgc tttccgggtc ggcgtagccg gcgatttcca gcttcagcgc
      781 ggcacgctcg ctgagggcgc gggccagcgt ttccagcttc ttctgcgccg ccgcatcgaa
      841 ctgcgtgcgc cccggcgcga aggccagcgt ggacagttcc tcgccgccgc cgaacagcga
      901 gccgatcagc gcgaacggcg aggtcaccgc cttgacgaaa aggttgacga tgaccttgat
      961 caccagcccg ccgaccgaga attgcggatc gtcgagcgaa ccggaaatcg gcaggttgat
     1021 gtcgatctcg ccgcggttgt tgcgcagcag cgccagcgcc aggttgaccg ggagcttggt
     1081 ggcgtccggg ctatcgaccc gctcgccgaa ggtgaactgg tcgatgaaca ggcggttgtc
     1141 ggcgctcaac tggcggttct ccagtttgta ggcaacgttc agcgacagct tgcccttgtc
     1201 gatcaggtag ccggcgtact tgccggagta cggcgagaag ccggtcatgt cgacgccctt
     1261 cacctcgccc ttgagatcga ggtaagactg cgccgccagc gggttgaggc gggcgctgat
     1321 cagcaccggc gccgaaccgg cgtaggtgcc gcgcaattcc atgtcggcga gcgtgcccgc
     1381 ggccgacgac aggccgctga cccggccgtt cagcttgccg acgttgaccg tgtagttggg
     1441 cttgacgaag tagtcggaga aattgaccgt gccgttgttc agcgtcacct tggcgatgcg
     1501 gatcggcacc ggcggcgccg ccggtgtcgt agctgccgca accggcggcg gattcaccgg
     1561 cgctgccgct gggtcggcgg cggcgaccgg cgccgcttcg ttgcggcgaa cgatgtcggc
     1621 gaggttgagg cggccgtcct tgttgaggat caggcgcgag tagaaatcgg ccagcgcgat
     1681 ctcgcgcacg tcgatcgcca gcggatcgag gcggaaatcg acgccaccga aatgcagcga
     1741 tttccacttc aggaaatcgg ccttgttgtc cgggtccacc gccagcaggt tgccgatcgt
     1801 cgccgaaccc ttgtaggccg ccttcagccg gccgccgtcg acgcggacga cggcctcacc
     1861 ctggttcgag aattggccac gctgcaggcg gatgttgagg tagcggccgg aataggcctg
     1921 caggccctgc accgggatgc cgctgctgtc gatctgcagc ttggccgcca gcggcaccgc
     1981 ctggacttcg ccggccaccg ccagcttgcc ttcgccattg accgtcgcct gcaaggtcag
     2041 gcggcccttc tcgttcggca ggttgcccag gcgctcgccc tgcaggctca agccgctgat
     2101 ctcctgcacc gccgccggct gcaccgactg atcctcgaaa cgcaggccga ggtcgtccac
     2161 cttgagcttc gccacctgcg ccagccacgg ccgcggcgcc ggcgccttgc cgccctgagc
     2221 gacgccgcgc agcaccggtg gcgtcagcca ggcgaagctg ccgtcggcgg ccaggccagc
     2281 ggcgatgcgc gcgccgctga gcgccagttc gccgatctcg acgcgctgcg ccgccaggtc
     2341 gacgcgcagc gccttgaggc cgacgctgcc gactttcagc ggcgtgccga aatccatctg
     2401 ccagcccgcc tcggtaatct ccagtggtgc cgccagacga tgatcgacgg caccgagccg
     2461 ggcattcagc gcaccgatct cgccgctcac tggctgtggc ccggaggcgt cgcgccagcg
     2521 gacgcgcgca tcgttcagtt cgaaagccgc cagcgaccag gcgaacggcg ttgcccgtgg
     2581 cgttgcggac ggcgctgctg ccgccggcct ggcgagccgg ttggcgacgg cgagcaggtt
     2641 gagttcgccg cgcccgtcga ccgccagatc gagttcggcg ccgtccagcg taaccttgcg
     2701 cagcgccaga ttgcgcttga acggatcgaa ctgctcgaga tcgacaagca gcgatttcca
     2761 gccgaccagc ggccggccac catcgtcgct cagcgccagc ccgtcgaggc gcgcctgacc
     2821 ggtcagggtc agcgaattgg cgccgtcggt cagttcccgg aacaccaggc gcaactgcga
     2881 atgcagcctg gcggcggcga tccgcagcgg cacaccggcc ggcagatagg gttgcagttg
     2941 cgccaggtcg aagccatcga gttgcaggtt cagctcgctc tcg
//
LOCUS       DAOSDX010000301         2979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11679_length_2979_cov_3.690150, whole genome shotgun sequence.
ACCESSION   DAOSDX010000301 DAOSDX010000000
VERSION     DAOSDX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11679_length_2979_cov_3.690150"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLN02_07575"
     CDS             <1..224
                     /locus_tag="PLN02_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOI52221.1"
                     /translation="LLDETLKAAATIAGYSLPVVMMIKESVNRAFESSLNEGLLFERR
                     VFHAAFALEDQKEGMAAFVEKRKPAFKNR"
     gene            complement(228..833)
                     /locus_tag="PLN02_07580"
     CDS             complement(228..833)
                     /locus_tag="PLN02_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOI52222.1"
                     /translation="MTTDPDVFTILQRFAPASPPNRLADSVLLIIDAQREYLDGSLAL
                     PGIGPALAAGARLLARARAAGAPVVHVLHRGAGPIFNPETPAYRPLDEMQPLPGESVV
                     NKTLADAFAGTELQAVLAATGRQNLIVIGFMTHNCVSSTVRAATGRGFRCTVVAPACA
                     TRPLPDGQGGVMAAELLHEASLVGLSDTQAVIVREVAAIPD"
     gene            926..>1736
                     /locus_tag="PLN02_07585"
     CDS             926..>1736
                     /locus_tag="PLN02_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52223.1"
                     /translation="MTALALLVAPGSTPSSVTSTLDMVAIARRHPAAADCRLDLLSAS
                     GGEIALAPALRLQTTALPERLDDYAAILISGFFAADIADLVRQLAAVWQPAIQRLAAL
                     PADRLVAASCYGSFVLAESGRLDGRPATTPWWLADAFHQRYPQVRLDADQALVDAGPA
                     ITAGAMTAHVDLSLHVLRRLFGAPLAREVGGIMLIDGARRSQRPFKSLPQRFADPLVD
                     AAAGWLGRHASQDVSTQELAAALAVSYRTLHRRFVAAAGMPPLAYLQALRIE"
     assembly_gap    1737..1746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1927..>2979
                     /locus_tag="PLN02_07590"
     CDS             1927..>2979
                     /locus_tag="PLN02_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52224.1"
                     /translation="MHLFADITAHGFGHLAISAPVLNALAELAPELRLTVRSRLPLAK
                     LRERIAPPFTLIEAASDFGYVMHDALRVDREASAAAYRAAHADWPARVAAEAEFLRSL
                     APDAVLSNVAYLPLAGAKLAGIPALAICSLNWADLFAHHFPGDPWAPPIHAQILAAYR
                     DTDTFLRLTPGMPMHDLARVEPIAPIAVRGTRHDLGFGAGRKILIALGGFDHRLPVDD
                     WPRLPATRWLVPASWQCRHPDAVAFDDRGLSFTDLLASVDAVITKPGYGSFAEAACCG
                     TPVLYLRRDDWPEQDCLIDWLEQQGRCREIAADRLRRGDIVPDLEALLALPAPPCPQP
                     DGALQAAHRILATVQGG"
BASE COUNT          432 a         1110 c          968 g          459 t
ORIGIN      
        1 aactgctgga cgaaacgctg aaggccgccg cgaccatcgc cggctactcg ctgccagtgg
       61 tgatgatgat caaggagtcg gtcaaccgcg ccttcgagtc ctcgctcaac gagggcctgc
      121 tcttcgaacg ccgcgttttc cacgcggcct tcgccctcga agatcagaag gaaggcatgg
      181 ccgccttcgt cgagaagcgc aagccggcct tcaagaaccg ctgaggttca gtcgggaata
      241 gcagcgacct cgcggacgat caccgcctgc gtgtcgctga ggccgaccag gctggcctcg
      301 tgcagcaact cggcggccat caccccgccc tgcccgtccg gcagcgggcg ggtagcgcag
      361 gccggggcga cgacggtgca ccggaaaccg cgcccggtcg ccgcgcgcac cgtcgaactg
      421 acgcagttgt gggtcatgaa gccgatgacg atcaggttct gccgccctgt ggctgccagc
      481 accgcctgca gttcggtacc ggcgaaggcg tcggccagcg tcttgttgac cacgctttcg
      541 ccgggcagcg gctgcatctc gtcgagcggc cggtaagctg gcgtttccgg gttgaagatc
      601 ggcccggcgc cgcgatgcag gacgtgcacg accggtgcgc cggcagcgcg agcacgcgcc
      661 agcaagcggg cgccggcagc cagcgcgggg ccgatgccgg gcagcgccaa cgagccgtcg
      721 aggtattcgc gctgcgcgtc gatgatcagc agcacgctgt cggcgagccg attcggcggg
      781 cttgccgggg cgaaacgctg gaggatggtg aaaacgtccg ggtcggtggt catggcaatc
      841 tccgtgtaag tgggcaaggc aatgatgcgc gtcggcaaaa ggcgccgaaa gcggcataat
      901 tgacattctt catgccgaat ctgccatgac cgccctcgcc ctcctcgtcg cccccggctc
      961 cacgccatcg agcgtcacca gcacgctgga catggtcgcc atcgcccgcc gtcatccggc
     1021 cgccgccgac tgccgactcg acctgctgtc ggccagcggc ggcgagatcg ccctggcgcc
     1081 ggcgctccgt ttgcagacga cggcgctgcc ggagcgcctg gacgactacg cggcgatcct
     1141 catctccggc ttctttgcgg cggatatcgc cgacctggtg cgccagctcg ctgccgtctg
     1201 gcagccagct attcagcggc tggcggcgct gccggcggat cgcctggtcg ccgccagttg
     1261 ctacggcagc ttcgtcctcg ccgaaagcgg tcgcctcgac gggcgtccgg caaccacccc
     1321 gtggtggctg gccgacgcct tccaccagcg ctatccgcag gtccgactgg atgccgacca
     1381 ggcgctggtc gacgccggcc cggccatcac tgccggggcg atgacggcgc atgtcgacct
     1441 gtcgctgcat gtgttgcgcc gcctgttcgg cgccccgctt gcccgcgagg ttggcgggat
     1501 catgctgatc gacggcgccc gccgttcgca gcggccgttc aagtccctgc cgcagcgctt
     1561 cgccgatccg ctggtcgacg ccgccgccgg ctggctgggc cggcacgcca gccaggatgt
     1621 cagcacccag gaactcgccg ccgcgctagc cgtcagctac cgcaccctgc accggcgttt
     1681 cgtcgccgcc gccggcatgc cgccgctcgc ctacctgcag gcgctgcgca tcgaacnnnn
     1741 nnnnnncacg cccgcgaact gctggaaacg acccgcgaca gcatcgaacg catcgtcgaa
     1801 gcggtcggct accgcgacag ttctgccttc cgccgcctgt tcgcgcgcca gctcgggctc
     1861 agcccggccg agtatcgcca gcgcttctgc caccccgagc ccacttttcc ggagaccgac
     1921 tgaaccatgc atctgtttgc cgacatcacc gcccacggct tcggccacct ggcgatcagc
     1981 gcgccggtcc tcaacgcgct ggcggaactc gcgcccgagc tgcgcctgac cgtgcgcagc
     2041 cggctaccgc tcgccaaact gcgcgagcgg atcgccccgc ccttcaccct gatcgaagcc
     2101 gccagcgatt tcggctacgt gatgcacgac gccttgcgcg tcgaccggga ggccagcgcc
     2161 gccgcctacc gcgccgcgca cgccgactgg ccggcgcggg ttgcggcgga ggcggaattc
     2221 ctgcgttcgc tggcgccgga cgcggtgctc agcaacgttg cctacctgcc gctcgctggg
     2281 gcgaagctgg ccggcattcc ggcgctggcg atctgctcgc tgaactgggc cgacctgttt
     2341 gcccaccatt ttcccggcga cccctgggcg ccgccgatcc atgcccagat tctcgccgcc
     2401 taccgcgaca ccgacacctt cctgcgcctg acaccaggga tgccgatgca cgacctggcc
     2461 cgcgtcgaac cgatcgcccc gatcgccgtc cgcggcaccc ggcacgatct cggattcggc
     2521 gccgggcgca agatcctgat cgccctcggc ggcttcgacc atcgcctgcc ggtcgacgac
     2581 tggccgcggc tgcccgccac ccgctggctg gtaccggcca gctggcaatg ccgccacccg
     2641 gacgcggtcg ccttcgatga ccgtgggctg agtttcaccg acctgctcgc ctcggtcgat
     2701 gccgtgatca ccaagccagg ctatggttct ttcgccgaag ccgcctgctg cggcacaccg
     2761 gtgctttacc tgcgacgcga cgactggccg gagcaggatt gcctgatcga ctggctggag
     2821 cagcaaggcc gctgccgcga aattgccgcc gaccgcctgc gccggggcga catcgtgccc
     2881 gacctggaag ccctgctggc attgccggca ccaccttgcc cgcagccgga cggcgcactc
     2941 caggcggcgc accgcatcct ggcgacagtc cagggcggg
//
LOCUS       DAOSDX010000302         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11685_length_2977_cov_3.560233, whole genome shotgun sequence.
ACCESSION   DAOSDX010000302 DAOSDX010000000
VERSION     DAOSDX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11685_length_2977_cov_3.560233"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLN02_07595"
     CDS             complement(<1..1044)
                     /locus_tag="PLN02_07595"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOI52225.1"
                     /translation="MTTFLRMTEVELAGKRVLIRADLNVPTDDAGRITDETRIVASLA
                     AMRLALDAGAAVMVTSHFGRPKEGALQPGDTLAPVAERLAEALGRPVPLVRNWLADGW
                     SVKPGQIVLLENCRGNVGEKADDPALAERIAALCDVYVNDAFGAAHRAEATTQGVARY
                     APLACAGPLLAAELDALGQAFEAPRRPLVAIVGGSKVSTKLSILRSLAARVDRLIVGG
                     GIANTFLLAAGKPIGKSLAEPDLVDEARAIMAMMAARGASVPLPEDVVVAGEFSPSAE
                     ARLCPVDAVAADEMIFDVGPRSAREIAGILQGAGTIVWNGPLGVFEFPQFAAGTAALA
                     RAIGIEHVQAVDPF"
     gene            complement(1055..2059)
                     /gene="gap"
                     /locus_tag="PLN02_07600"
     CDS             complement(1055..2059)
                     /gene="gap"
                     /locus_tag="PLN02_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOI52226.1"
                     /translation="MPIRVAINGFGRIGRMALRAIAQEDEFSGLEVVGINDLLDADYL
                     AYLLKHDSVHGRFAGEVAVDGGDLLVDGQRIRLSAERDPARLNWGELGVDLVIEATGF
                     FLTQESCQAHIAAGARKVVQSAPSKDDTPMFVYGVNHDSYAGQAILSAASCTTNCLAP
                     VAKVLNDRFGIKRGLMSTVHAATATQKTVDGPSGKDWRGGRGILDNIIPSSTGAAKAV
                     GKVIPALAGRLTGMAFRVPTPDVSVVDLTVELASSASYAEICEAMREAAAGPLKGVLA
                     YTDQAVVSTDFRGCPQPSIFDAEAGIALDPSFVKVVSWYDNEYGYTCNMLRLARHVAH
                     "
     gene            complement(2106..>2977)
                     /locus_tag="PLN02_07605"
     CDS             complement(2106..>2977)
                     /locus_tag="PLN02_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOI52227.1"
                     /translation="NLTAGKGSVAWHAAGDKTANYLSYGVREFGMAAIMNGVALHGGL
                     IPYGGTFAVFSDYSRNAIRMSALMQQRVVHVLTHDSIGLGEDGPTHQPIEHVGSLRLI
                     PGLDVWRPCDELETAVAWAEALERRDGPSALFLSRQNLDQCGDRPGRADEIARGGYVL
                     READGPLQAVILATGSEVGIALAAQRELAAHGVAVRVVSMPCCERFDRQKTAWKKAVL
                     PPDVVRIAIEAAHPDPWRKYVGLVGEVVGIDSFGASAPAPVLYEHFKLTAAHLASVVV
                     ETIARESGSDGDF"
BASE COUNT          469 a         1002 c         1057 g          449 t
ORIGIN      
        1 gaacggatcc acggcctgaa cgtgctcgat gccgatggcg cgggccagcg ccgcggtgcc
       61 ggcggcaaat tgcgggaatt cgaagacgcc gagcggcccg ttccagacga tggtgccggc
      121 gccctgcagg atgccggcaa tctcacgcgc gctgcgcggg ccaacgtcga agatcatctc
      181 gtcggcagcg accgcgtcga ccgggcacaa gcgggcctcg gcgctcggcg aaaactcgcc
      241 ggcgacaacg acgtcctcgg gcagcggaac ggacgcgccg cgcgccgcca tcatggccat
      301 gatcgcccgc gcctcatcga ccaggtcggg ttcggccagc gatttgccga tcggcttgcc
      361 ggcggcgagc aggaaggtgt tggcgatgcc gccgccgacg atcaggcgat cgacgcgggc
      421 agcgagcgaa cgcaggatgg acagcttggt ggacaccttg gaaccgccga cgatcgccac
      481 cagcggccgg cgcggcgcct cgaaggcctg gccgagggca tcgagttcgg cggccagcag
      541 tgggccggca caggccagcg gcgcgtagcg ggcgacgcct tgggtagtcg cctcggcgcg
      601 gtgggcggcg ccgaaggcgt cgttgacata gacgtcgcag agcgcggcga tgcgttcggc
      661 gagcgccgga tcgtcggcct tctcgccgac gttgccgcgg cagttctcga gcaggacgat
      721 ctgcccgggc ttgacgctcc agccgtcggc cagccagttc ctgaccagcg gcaccggccg
      781 accaagcgct tcggccaggc gttcggcgac cggcgccagg gtgtcgcccg gttgcagcgc
      841 gccctccttc ggacggccga aatgcgaggt caccatcacc gccgcgccgg cgtccagcgc
      901 cagccgcatc gccgccagcg aggcgacgat gcgcgtctcg tcggtgatcc ggccggcgtc
      961 gtcggtcggc acgttgaggt cggcgcggat cagcactcgc ttgccggcaa gctcgacctc
     1021 ggtcatgcgc agaaaagtcg tcatcgccgc cccctcagtg cgcgacatgg cgcgccaggc
     1081 gcagcatgtt gcaggtgtag ccgtactcgt tgtcgtacca cgagacgacc ttgacgaagc
     1141 tgggatcgag cgcgatgccg gcctcggcgt cgaagatcga gggttgcggg cagccgcgga
     1201 aatcggtcga taccaccgcc tggtcggtat aggcgaggac acccttgagc ggtccggcgg
     1261 cggcttcgcg catggcctcg cagatctcgg catacgacgc gctgctcgcc agttcgacgg
     1321 tcaggtcgac caccgagacg tccggcgtcg gcacccggaa ggccatcccg gtcaggcgcc
     1381 cggccagcgc cggaatcacc ttgccgacgg ccttggcggc gccggtcgag gacggaatga
     1441 tgttgtccag gatgccgcgc ccgccgcgcc agtccttgcc ggacgggccg tcgaccgtct
     1501 tctgggtggc ggtcgccgca tgcaccgtgc tcatcaggcc gcgtttgatg ccgaaacggt
     1561 cgttgagcac cttggccacc ggcgccaggc agttggtcgt gcaagacgct gccgaaagaa
     1621 tcgcctgacc ggcgtagctg tcgtgattga cgccatagac gaacatcggc gtgtcgtcct
     1681 tcgacggcgc cgactgcacg accttgcgcg ctccggcggc gatatgcgcc tgacagcttt
     1741 cctgggtgag gaagaagccg gtcgcctcga tcaccagatc gacgccgagt tcgccccagt
     1801 tcagccgggc cgggtcacgt tcggcgctga gccggatgcg ctggccatcg accagcaggt
     1861 cgccgccatc gacagcgacc tcgccggcga aacggccgtg caccgagtcg tgtttgagca
     1921 gataggcgag gtagtcggcg tcgagcaggt cgttgatgcc gaccacctcg agaccggaaa
     1981 actcgtcttc ctgggcgatg gcgcgcaagg ccatgcgtcc gatacggccg aagccgttga
     2041 ttgccacccg tattggcatg ggttgtctcc tgattttgaa tgaaaatcgg tggctgccgg
     2101 ggcgctcaga agtcgccgtc actgccgctc tcgcgggcaa tggtttcgac gacgacgctc
     2161 gccagatgcg cggcggtcag cttgaaatgt tcgtagagga cgggggccgg ggcggaggcg
     2221 ccgaagctgt cgatgccgac cacttcgccg accaggccga cgtacttgcg ccagggatcg
     2281 ggatgcgccg cctcgatggc gatgcgcacc acgtccggcg gcagcaccgc cttcttccac
     2341 gccgtcttct ggcggtcgaa gcgctcgcag catggcatcg agacgacgcg cacggcgacg
     2401 ccgtgggcag ccagctcgcg ctgcgcggcc agcgcgatgc cgacctccga gccggtggcg
     2461 aggatcaccg cctgcagcgg gccgtccgct tcgcgcagga cgtagccgcc gcgggcaatc
     2521 tcatcggccc ggccggggcg gtcgccgcat tggtcgagat tctgccgcga caggaacagg
     2581 gcgctcggcc catcgcggcg ctccagcgcc tcggcccagg cgaccgccgt ttccagttcg
     2641 tcgcaggggc gccagacatc gagaccggga atcaggcgca ggctgccgac atgctcgatc
     2701 ggctggtgcg tcgggccgtc ctcgccgaga ccgatcgaat cgtgggtcag gacatggacg
     2761 acgcgctgct gcatcagcgc actcatgcgg atggcgttgc gcgagtaatc ggagaagacg
     2821 gcgaaggtgc cgccgtaggg aatcagcccg ccgtgcaggg cgacgccgtt catgatcgcc
     2881 gccatgccga attcgcgcac gccgtaagac aggtagttcg ccgtcttgtc gccggccgcg
     2941 tgccaggcga cgctgccttt gccggcggtc aggttgg
//
LOCUS       DAOSDX010000303         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11705_length_2975_cov_3.253767, whole genome shotgun sequence.
ACCESSION   DAOSDX010000303 DAOSDX010000000
VERSION     DAOSDX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11705_length_2975_cov_3.253767"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /gene="leuB"
                     /locus_tag="PLN02_07610"
     CDS             complement(<1..860)
                     /gene="leuB"
                     /locus_tag="PLN02_07610"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOI52228.1"
                     /translation="MKICVLPGDGIGPEITAEAVRVLKALDLKFEMEEALLGGCAVDA
                     TGNPYPEATQKLAREADAILLGAVGGPQWDSLPREKRPERGLLGIRKDLNLFANLRPA
                     NASTLKAEVVAGLDILIVRELTGDIYFGQPRGVREENGERVGFNTMVYSESEIRRIGH
                     VAFQAAQKRNKRLCSVDKMNVLECTQLWRDVMIEVSKDYPDVELSHMLVDNAAMQLVK
                     APKQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLNTKNQGLYEPSHGSAPDIAGK
                     DMANPCSLILS"
     gene            complement(870..1511)
                     /gene="leuD"
                     /locus_tag="PLN02_07615"
     CDS             complement(870..1511)
                     /gene="leuD"
                     /locus_tag="PLN02_07615"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOI52229.1"
                     /translation="MDKFVRLDGLVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYMDVGQPGQDNSQRPKNPNFVLNQPRYQGAEVLLTRANFGCGSSREHAPWALLDY
                     GFKAIIAESFADIFFNNCFKNGILPIVLPAFEVEALFQQVEATPGYKLTVDLPAQAVV
                     RPDGHAIPFQVDAFRKECLLNGWDDIGLTLRHADKIKAFEEKRRVEQPWLFAQ"
     gene            complement(1522..1653)
                     /locus_tag="PLN02_07620"
     CDS             complement(1522..1653)
                     /locus_tag="PLN02_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HOI52230.1"
                     /translation="MMKKAFVLLLAVAGLVSAMGCNTVQGIGKDIEKGGEAIQRSTK"
     gene            complement(1663..>2975)
                     /gene="leuC"
                     /locus_tag="PLN02_07625"
     CDS             complement(1663..>2975)
                     /gene="leuC"
                     /locus_tag="PLN02_07625"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOI52231.1"
                     /translation="HEVTSPQAFEGLKLAGRKPWRVSSIVSTPDHNTPTDHWEEGIKD
                     PISRLQVETLDANIREVGALAYFPFKDPRMGILHVVGPENGATLPGMTVVCGDSHTST
                     HGAFACLAHGIGTSEVEHVLATQCLVQKKSKTMLIAVEGRLGKGVTAKDVALAIIGAI
                     GTAGGTGYAVEFGGSAIRSLSMEGRMTLCNMAIEGGARMGFVAVDDTTINYLKGRPFS
                     PRGETWDKAVAYWRTLHSDPGARFDRVITLKAEEIRPQVTWGTSPEMVVGIDAVVPDP
                     AKEADPVKREGMERALQYMGLNPNVPLKSIPVDKVFIGSCTNSRIEDLRQAAAVLKGR
                     HVAANIKLALAVPGSGLVKRQAEAEGLDKVFIAAGFEWREPGCSMCLAMNADRLEPGE
                     RCASTSNRNFEGRQGPGGRTHLVSPAMAAAAAVAGRFADVRDIL"
BASE COUNT          506 a          944 c          961 g          564 t
ORIGIN      
        1 gaaaggatca gtgaacaggg attggccatg tccttgccgg cgatgtctgg agcgctgccg
       61 tggctgggct cgtacaggcc ctggttcttc gtgttcagcg aggccgaggg cagcatgccg
      121 atcgagccgg tgagcatcga cgcctcgtcg gacaggatgt cgccgaacat gttgccggtg
      181 accatcacgt cgaactgctt cggcgccttc accagttgca tggcggcgtt atcgaccagc
      241 atgtggctga gttcgacgtc cgggtagtcc ttgctcactt cgatcatcac gtcgcgccag
      301 agttgcgtgc attcgaggac gttcatcttg tccaccgagc acaggcgctt gttgcgcttc
      361 tgcgccgcct ggaaggcaac gtggccgatg cggcggattt ccgactcgct atagaccatg
      421 gtgttgaagc cgacgcgctc gccgttttcc tcgcgcacgc cgcgcggctg accgaagtag
      481 atgtcgccgg tcagttcgcg gacgatcagg atgtccagcc cggcgacgac ttccgccttc
      541 agcgtcgagg cattggccgg acgcaggttg gcgaacaggt tgagatcctt gcggatgccg
      601 agcaggccgc gctccgggcg cttctcgcgg ggcaggctgt cccactgcgg gccgccgacg
      661 gcgccgagca ggatggcgtc ggcttcgcgc gccagtttct gcgtcgcctc cggataggga
      721 ttgcccgtgg cgtcgacggc acagccgccg agcagcgctt cttccatctc gaacttgagg
      781 tcgagcgcct tgagcacgcg caccgcttcg gcggtgattt ccggaccgat gccgtcaccc
      841 ggcagcacac aaattttcat gttgtctcct tattgggcga acagccaggg ctgctcgacg
      901 cggcgcttct cttcgaaggc cttgatcttg tcggcgtgac gcagggtcag gccgatgtcg
      961 tcccagccgt tgagcaggca ttccttgcgg aatgcgtcga cctggaaggg aatggcgtga
     1021 ccgtcggggc gtaccaccgc ctgcgccggc aggtctacgg tcaacttgta gcccggcgtt
     1081 gcctcgacct gctggaacag cgcctcgact tcgaaggccg gcaggacgat cggcaggatg
     1141 ccgttcttga agcagttgtt gaagaagatg tcggcaaacg actcggcgat gatcgccttg
     1201 aaaccgtagt cgagcagtgc ccagggcgcg tgctcgcgcg aactgccgca gccgaagttg
     1261 gcgcgcgtca gcaacacttc ggcaccctgg tagcgcggct ggttgagcac gaagttgggg
     1321 ttcttcgggc gctgcgaatt atcctggccg ggctggccga catccatgta gcgccactcg
     1381 tcgaaggcgt tcgggccgaa accggaacgc ttgatcgact tcaggaactg cttcgggatg
     1441 atcgcgtcgg tatcgacgtt gctgcggtcg agcggcgcca ccagcccgtc gaggcgtacg
     1501 aacttgtcca tgaaccttgc cttacttggt cgaacgctgg atggcttcgc cacccttctc
     1561 aatatccttg ccgattccct ggacggtgtt gcagcccatt gcggaaacca gaccagccac
     1621 ggccaacagc agaacaaacg ctttcttcat catcggttcc tttcagagaa tatcgcggac
     1681 gtcagcaaaa cggccggcca ccgcggcagc ggcagccatc gccgggctga ccaggtgagt
     1741 acgaccgcca ggaccctggc ggccttcgaa gttacggttc gaggtcgagg cgcaacgctc
     1801 gcccggttcc agacggtcgg cgttcatcgc cagacacatc gagcagcccg gctcgcgcca
     1861 ttcgaaaccg gcggcaatga agaccttgtc cagcccctcc gcctcggcct ggcgcttgac
     1921 cagaccggaa cccggcacgg ccagcgccag cttgatgttg gccgcgacgt gacgcccctt
     1981 cagcacggca gccgcctggc gcagatcctc gatgcgggag ttggtacagg aaccaatgaa
     2041 cactttatcg acgggaatgg atttgagcgg aacgttggga ttgagaccca tgtattgcag
     2101 cgcgcgttcc attccctcgc gcttgaccgg atcggcttcc ttcgccggat cgggcacgac
     2161 agcgtcgatg ccgaccacca tttcgggcga cgtgccccag gtcacctgcg gccgaatctc
     2221 ttcggccttg agggtgatca cgcggtcgaa gcgggcgccc ggatcggaat gcagggtgcg
     2281 ccagtaggca acggccttgt cccaggtttc gccacgcggc gagaacggac ggcccttcag
     2341 ataattgatc gtggtgtcgt cgacggcgac gaaacccatc cgggcgccgc cttcgatcgc
     2401 catgttgcac agcgtcatgc gcccttccat cgacaggctg cggatcgcgc tgccgccgaa
     2461 ttcgacggca tagccggtac cgccagcggt accgatggcg ccgatgatgg ccagcgccac
     2521 gtccttggcg gtgacgccct tgcccaggcg cccctcgacg gcgatcagca tggtcttcga
     2581 cttcttttgc accaggcact gcgtcgccag cacatgctcg acctcggagg taccgatgcc
     2641 gtgcgccaga caggcgaagg cgccatgcgt cgaggtgtgc gaatcgccgc agacgacggt
     2701 catgcccggc agcgtggcgc cgttttccgg gccgacgacg tgcaggatgc ccatccgcgg
     2761 atccttgaac gggaaatagg cgagcgcgcc gacttcgcgg atgttggcgt cgagcgtctc
     2821 gacctgcagg cgggagatcg gatccttgat cccctcttcc cagtggtcgg tcggcgtgtt
     2881 gtgatccggc gtcgacacga tggaagaaac gcgccacggc ttgcgtccgg ccagcttcag
     2941 gccctcgaag gcctgcggac tggtgacttc gtgga
//
LOCUS       DAOSDX010000304         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11709_length_2974_cov_3.321686, whole genome shotgun sequence.
ACCESSION   DAOSDX010000304 DAOSDX010000000
VERSION     DAOSDX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_2974_cov_3.321686"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..952
                     /locus_tag="PLN02_07630"
     CDS             107..952
                     /locus_tag="PLN02_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOI52232.1"
                     /translation="MRFSGTDTYVAPRDLTLAVNAAIALQRPLLIKGEPGTGKTLLAE
                     EVAGALGLPLFQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDPRVADIAHYIVPGVLWQ
                     AFECDTPSVVLIDEIDKADIEFPNDLLRELDRMEFHCYELQKTIRARHRPLIVITSNN
                     EKELPDAFLRRCFFHYIKFPDRETMQRIVAVHFPTLKQELLREALEVFFELREVPGLK
                     KKPSTSELIDWLKLLLAEDIPPEALRAKDAKDAIPPLHGALLKNEQDIHLFERLAFMA
                     RRKTP"
     gene            960..2144
                     /locus_tag="PLN02_07635"
     CDS             960..2144
                     /locus_tag="PLN02_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOI52233.1"
                     /translation="MLVDFFLHLKARQIPVSTQELLALLEALQARLVGPALDDFYLLA
                     RTLLVKDESHYDRFDRAFGEYFKGIEQLPGLDAEIPEDWLRLAAQRHLSEEERKKLEQ
                     LSYEKIFERLRERLAEQKERHAGGNKWIGTGGTSPFGHGGYNPAGVRIGGDSAGNRTA
                     IKVWEQRAYRNLDDQVELGTRNIKVALRRLRRFARQGAANELDLDGTIAGTARNGGWL
                     DLHLRPERHNAVKVLLFLDVGGSMDDHIQVCEELFSAARAEFKHLEHFYFHNCVYEHV
                     WRDNRRRHDQRLSTLDLIHTYGRDYTLIFVGDATMSPYEVDHPGGSVEHWNEESGAVW
                     LQRLVAAWPRVAWLNPVDENYWQYTPSIAAIRRQIGDERMFPLTLDGLERAMRRLASA
                     RG"
     gene            complement(2157..2535)
                     /gene="rnpB"
                     /locus_tag="PLN02_07640"
     ncRNA           complement(2157..2535)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLN02_07640"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2565..2741)
                     /locus_tag="PLN02_07645"
     CDS             complement(2565..2741)
                     /locus_tag="PLN02_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52234.1"
                     /translation="MKQPSSSERRRLDRRRSPVTPPPYRTAAGLVLAERRARVDRRSA
                     WVRDIVVETDGETS"
     gene            complement(2799..>2974)
                     /locus_tag="PLN02_07650"
     CDS             complement(2799..>2974)
                     /locus_tag="PLN02_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOI52235.1"
                     /translation="LLITGQQSDYQLKVVVRDMDAYQDLLLNKITRIPGVTGVHTSFV
                     LRKVVDRGALPVD"
BASE COUNT          541 a         1056 c          899 g          478 t
ORIGIN      
        1 ggcatcggac caggagattc ggggcgggct gatttcggac atggcgcatt gtaggccagc
       61 cgcttgccct tacaatcctt ccaccccaac ccgccggacc ccgaccatgc gtttctcagg
      121 caccgacacc tatgtcgccc cgcgcgacct gaccctcgcc gtcaatgccg ccatcgccct
      181 gcagcgaccg ctgctgatca agggcgaacc gggcaccggc aagaccctgc tcgccgagga
      241 ggtggccggc gcgctcggcc tgccgctgtt tcagtggcac atcaagtcga ccaccaaggc
      301 gcagcagggc ctctacgaat acgacgccgt ctcgcgcctg cgcgactcgc aactgggcga
      361 cccgcgcgtc gccgacatcg cccactacat cgtccccggc gtgctgtggc aggccttcga
      421 atgcgacacg ccgtcggtgg tgctgatcga cgagatcgac aaggccgaca tcgaatttcc
      481 caacgacctg ctgcgcgaac tcgaccgcat ggagttccac tgctacgagc tgcagaagac
      541 gatccgcgcc cgccaccggc cgctgatcgt catcacctcg aacaacgaga aggaactgcc
      601 cgacgccttc ctgcgccgct gcttcttcca ctacatcaag ttccccgacc gcgagacgat
      661 gcagcgcatc gtcgccgtgc atttcccgac cctcaagcag gaactgctgc gcgaggcgct
      721 ggaagtcttc ttcgagctgc gcgaggtgcc cggcctcaag aagaagccgt cgaccagcga
      781 actgatcgac tggctcaagc tgctcttggc cgaggacatc ccgcccgaag cgctgcgcgc
      841 caaggacgcc aaggacgcga tcccgccgct gcacggcgcg ctgctgaaga acgagcagga
      901 catccacctg ttcgagcggc tcgccttcat ggcgcggcgc aagaccccct gaaaccgcga
      961 tgctggtcga cttcttcctg cacctgaagg cgcggcagat tccggtgtcc acgcaggaac
     1021 tgctggccct gctcgaagcg ctgcaggcgc gcctggtcgg gccggcgctc gacgacttct
     1081 acctgctcgc gcgcaccttg ctggtcaagg acgagagcca ttacgaccgc ttcgaccgcg
     1141 ctttcggcga atacttcaag ggcatcgagc aactgcccgg actggatgcc gagatcccgg
     1201 aagactggtt gcgcctggcg gcgcaacgcc acctttccga agaagagcgg aaaaagctcg
     1261 aacaactgag ctacgagaaa atcttcgagc gcctgcgcga acgcctcgcc gaacagaagg
     1321 aacgccacgc cggcggcaac aagtggatcg gcaccggcgg cacctcgcct ttcggccacg
     1381 gcggctacaa cccggccggc gtacgcatcg gcggcgactc tgccggcaac cggacggcga
     1441 tcaaggtctg ggaacagcgc gcctaccgca acctcgacga ccaggtcgaa ctcggcaccc
     1501 gcaacatcaa ggtcgccctg cgtcggctgc gccgcttcgc ccgccagggt gcggcgaacg
     1561 aacttgacct cgacggcacc atcgccggca ccgcccgcaa cggcggctgg ctcgacctgc
     1621 acctgcggcc agaacgccac aacgcggtca aggtgctgct cttcctcgac gtcggcggct
     1681 cgatggacga tcacatccag gtctgcgagg aactcttctc cgccgcccgc gccgagttca
     1741 agcacctcga acatttctac ttccacaact gcgtctacga gcatgtctgg cgcgacaacc
     1801 ggcgccgcca cgaccaacgc ctgtccacgc tcgacctgat ccacacctac ggccgcgact
     1861 acaccttgat cttcgtcggc gacgcgacga tgagccccta cgaggtagac cacccgggcg
     1921 gcagcgtcga acactggaac gaggaaagcg gcgccgtctg gctgcagcgc ctggtcgccg
     1981 cctggccgcg cgtcgcctgg ctcaacccgg tggacgaaaa ctactggcaa tacaccccgt
     2041 cgatcgccgc catccgccgc cagatcggcg acgaacgcat gttcccgctc accctcgacg
     2101 ggctggagcg ggcgatgcgc cggctggcca gcgcgcgcgg ctgaaacgga aaactggtgg
     2161 aaagccggcc gataagccgg gttctgttcc cctcttgcga ggttcggcaa tcattcctct
     2221 aggcctgccg tcaccgacag gctcaagcaa cctacccgga agcagcgcgg gccacgcctc
     2281 agcttcccta tttggtcttg ctccggatgg ggtttagcgt gccgtcgaac gttaccgccg
     2341 acgcggtggg ctcttacccc gccgtttcac ccttacctgc cgggttgccc cgacaggcgg
     2401 tctactctct gttccacttt ccgttgcctt gggtcttgcg acttatagca cccagccgtt
     2461 agctggcatc ccgcccaatg gagcccggac tttcctcccg acacttgcgt gtccagcgat
     2521 tgcctggccg actttccgcg gcggattata cgtgggatcg gcgctcagct ggtttcgccg
     2581 tcggtttcga cgacgatgtc gcgcacccag gcgctgcgcc ggtcaacgcg cgcccggcgc
     2641 tcggccagga cgaggccggc ggccgtccgg taggggggcg gggtgactgg ggaacgccgg
     2701 cggtcgaggc ggcgacgttc ggaagaagag ggctgtttca tggccggcta ccggaagaaa
     2761 gggacgacca tgagaatagc atgagcccgc gaccccgctc aatcgaccgg cagggcgccg
     2821 cgatcaacca ccttgcgcag cacgaaactg gtgtgcacgc cggtcactcc cgggatgcgc
     2881 gtgatcttgt tgagcagcag gtcctggtag gcatccatgt cgcgcaccac cactttcaac
     2941 tggtagtcgg actgctggcc ggtgatcagc aggc
//
LOCUS       DAOSDX010000305         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11748_length_2964_cov_2.176693, whole genome shotgun sequence.
ACCESSION   DAOSDX010000305 DAOSDX010000000
VERSION     DAOSDX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11748_length_2964_cov_2.176693"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLN02_07655"
     CDS             complement(<1..437)
                     /locus_tag="PLN02_07655"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOI52236.1"
                     /translation="MNNPFPSMTRTLPSLPLLFAMAGATGRGTAADAPSNPHFINSPA
                     ALKERIKTELRVTRPDFIVFVPTVTDAGVNDTGNEHFLVFDGPDGSLMAVWTQSSAES
                     QPDQHIAFARSADEGRTWSKPRIIAGPKKPGDGFMASWGYPLV"
     gene            complement(434..1345)
                     /locus_tag="PLN02_07660"
     CDS             complement(434..1345)
                     /locus_tag="PLN02_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HOI52237.1"
                     /translation="MSLHSAPTPARFLQSFIFASRWIQAPLYVGLIVAQLVYVWLFMV
                     ELSHLVSGALGPHKMTEADIMLIVLGLIDVVMIANLLIMVIVGGYETFVSRLELEDHP
                     DQPEWLSHVNAGVLKIKLSTAIIGISSIHLLKSFINAGNLPEHTLYWQVAAIIGAVTG
                     GWLADRWMRRTVRGRIYVSAIGMSLIVPAMFGVGYAPQTGLLGVAIAFLILFGLGWGF
                     FDCNNMPILCQIARPELRATGYGIMNLVSISCGGLADWGFGILRDRQVPLFGIFGIFA
                     SAAVISVVLVLLIRPRDLDAPQRTSTP"
     gene            1605..>2964
                     /locus_tag="PLN02_07665"
     CDS             1605..>2964
                     /locus_tag="PLN02_07665"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOI52238.1"
                     /translation="MSNESVQLYYPSDEIVKNAAVSGMDAYRALCAEAEADYEGFWAK
                     RARELITWKEPFTQVLDESNAPFYKWFADGKLNVSYNCLDRQVEAGKGDKVAIVFEAD
                     NGDVTKVTYQELLVKVCKMANLLKAKGVQKGDRVVIYLPMTVEGVVAMQACARIGATH
                     SIVFGGFSAQALRDRINDAGATLLITADGQFRGGKSLPLKAIADEAIALGGCDTLKGV
                     VVFKRTGMDVTMVAGRDEWLHEGLASQPETCEPEWVGAEHPLFLLYTSGSTGKPKGVQ
                     HSTGGYLLHAILTMKYTFDIKDSDTFWCTADIGWVTGHTYITYGPLACGATEIVFEGV
                     PTYPDAGRFWKMIQDHKVSIFYTAPTAIRSLIKAADANPAVHPKSYDLSSLRILGSVG
                     EPINPAAWEWYYQNVGGSRCPIVDTFWQTETGGHMITPLPGATPLVPGSCTLPFPGIQ
                     FAV"
BASE COUNT          570 a          916 c          953 g          525 t
ORIGIN      
        1 ctgaccagcg gatagcccca gcttgccatg aaaccgtcgc ccggcttctt cggccccgcg
       61 atgatgcgcg gcttcgacca tgtccgtccc tcgtccgcgc tccgcgcgaa cgcgatatgc
      121 tggtccggtt gcgactccgc ggaactctgc gtccagaccg ccatcagcga gccgtccggt
      181 ccgtcgaaga cgaggaagtg ttcgttgccg gtgtcgttca cgccggcgtc ggtgacggtg
      241 gggacgaaca cgatgaagtc cggccgggtc acgcgcagtt ccgtcttgat tcgctccttc
      301 aacgcggctg gcgagttaat gaagtggggg ttcgacggcg catcagcggc cgtgcccctg
      361 ccggttgcgc cggccatggc gaaaagcagc ggcagggatg gcagtgtccg tgtcatggat
      421 gggaacgggt tgttcatgga gtacttgttc gttgaggcgc atccaggtcg cgggggcgga
      481 tgaggaggac aagcacgacg gagatcacgg cggcgctggc gaagatgccg aagatgccaa
      541 agagcggaac ctgccggtcg cgcaggatgc cgaatcccca atcggccagg ccgccgcaac
      601 tgatgctgac gaggttcatg atgccgtacc cggtggcgcg caactcgggc cgcgcgatct
      661 ggcagaggat gggcatgttg ttgcagtcga agaagcccca gccgagtccg aagaggatga
      721 ggaaggcgat ggcgaccccg agcaatccgg tctggggcgc gtagcccacg ccgaacatgg
      781 ccggcacgat aagcgacatg ccgatggcgc tcacgtagat gcgtccccgg acggtgcggc
      841 gcatccagcg gtcggccagc cagccgccgg tcacggcgcc gatgatggcg gccacctgcc
      901 agtagagcgt gtgttcgggc aggttgccgg cgttgatgaa acttttcagc agatggatcg
      961 acgagatgcc gatgattgcc gtcgacagct tgatcttcag caccccggcg ttgacgtggc
     1021 tgagccattc cggctggtcc gggtggtcct cgagttcgag gcgcgagacg aaggtttcat
     1081 agccgccgac gatgaccatg atcagcaggt tggcgatcat cacgacgtcg atcaggccga
     1141 ggacgatcag catgatgtcg gcttcggtca tcttgtgcgg ccctaaggca ccggacacca
     1201 gatggctgag ttcgaccatg aacagccaga catagaccag ttgggcgacg atcaggccga
     1261 cgtaaagcgg cgcctggatc cagcggctgg cgaaaatgaa ggattgcagg aaacgggccg
     1321 gcgtgggggc gctatgcaag gacatatttg tcttatggga tggcgttggc ttgggggcgc
     1381 aaagtttagc atggcgcggc aggcttgccc gaaaccgccg aatgctgcgc cgcagcgtga
     1441 tttcgtaagg ccattgtaag attatcgaag tgtaattagg gcttgacgct gcgccgggga
     1501 acgaggtggc gacggcgcgc aaggcctagc tgcaatccgg gaatttcccg aggcccgacc
     1561 ggttgttttc aacacaaatg cgatgtaacg cagaggagaa gaatatgtcg aatgagtccg
     1621 tgcagcttta ttatccgtcc gacgaaatcg tcaagaacgc ggcggtttcg gggatggacg
     1681 cctaccgggc gctgtgcgcg gaagcggaag ccgactacga aggtttctgg gccaagcgtg
     1741 cccgcgaact gatcacctgg aaggaaccct tcacccaggt gctcgacgag tccaacgcac
     1801 cgttctacaa gtggtttgcc gacggcaagc tgaacgtctc ctacaactgt ctcgatcgtc
     1861 aggtcgaagc cggcaagggc gacaaggtcg ccatcgtctt cgaagccgac aacggcgacg
     1921 tcaccaaggt cacctatcag gaactgctgg tcaaggtctg caagatggcc aacctgctca
     1981 aggccaaggg tgtgcagaag ggcgaccgcg tcgtcatcta tctgccgatg accgttgaag
     2041 gcgtcgtcgc catgcaggcc tgcgcccgta tcggcgccac ccactcgatc gttttcggcg
     2101 gcttctccgc ccaggccctg cgcgaccgca tcaacgatgc cggcgccacc ctgctgatca
     2161 ccgccgacgg ccagttccgc ggcggcaagt ccttgccgct caaggcgatc gccgacgaag
     2221 ccatcgccct tggcggttgc gacaccctga agggcgtcgt cgtcttcaag cgcaccggca
     2281 tggacgtcac catggttgcc ggccgtgacg aatggctgca cgaaggtctg gccagccagc
     2341 cggaaacctg cgaaccggaa tgggtcggtg ccgaacaccc gctgttcctg ctctacacct
     2401 ctggctccac cggcaagccg aagggcgttc agcattccac cggcggctat ctgctgcacg
     2461 ccatcctgac catgaagtac accttcgaca tcaaggatag cgacaccttc tggtgtaccg
     2521 ccgacatcgg ctgggtcacc ggccacacct acatcaccta cggtccgctc gcctgcggcg
     2581 ccaccgaaat cgtcttcgaa ggcgtgccga cctatccgga cgccggccgt ttctggaaga
     2641 tgatccagga tcacaaggtc tccatcttct acaccgcgcc gaccgcgatc cgttcgctga
     2701 tcaaggccgc cgatgccaac ccggcggtgc atccgaagag ctacgacctg tccagcctgc
     2761 gcatcctcgg ttcggtcggc gagccgatca acccggccgc ctgggagtgg tactaccaga
     2821 acgtcggtgg ctcgcgctgc ccgatcgtcg ataccttctg gcaaaccgaa accggtggtc
     2881 acatgatcac cccgttgccg ggcgcgacgc cgctggtccc gggttcctgc acgctgccct
     2941 tcccgggcat ccagttcgcc gtgg
//
LOCUS       DAOSDX010000306         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11809_length_2953_cov_4.572119, whole genome shotgun sequence.
ACCESSION   DAOSDX010000306 DAOSDX010000000
VERSION     DAOSDX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11809_length_2953_cov_4.572119"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLN02_07670"
     CDS             <1..1105
                     /locus_tag="PLN02_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52239.1"
                     /translation="SAFLANMSHEIRTPLNAITGMAHLIRRGGLNPEQSERLDKLEAA
                     GNHLLDIINAVLDLSKIEAGKLTLEEIPVNIGSILSNIVSMLHSRAEDRGITLIVEPG
                     APAGKLLGDPTRLQQALLNYAANAIKFTSEGWVRLAAETVASEGDSALIRFTVTDTGV
                     GIEPQAQSRLFSAFEQADNSTTRKFGGTGLGLAITRKLAESMGGEVGVISTPGEGSCF
                     WFTARLRRDPDDRSQTETASSARPTDSLAGRRVLLAEDEPVNREIARMLLDEIGLDSA
                     CAEDGSIALQMAAAEHYDLILMDMQMPNMDGLEATRRIRLLPGYAGVPILAMTANAFA
                     EDKARCFEAGMNDFIAKPARPEDLFAKLQQWLQ"
     gene            complement(1164..2441)
                     /locus_tag="PLN02_07675"
     CDS             complement(1164..2441)
                     /locus_tag="PLN02_07675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOI52240.1"
                     /translation="MTSPSLIGSLAVTPVAAYRHEKTLFAILAVFAGLFWLLLTVGTI
                     GIVWLYMLLMYLFALFAHSALISYLQGNAVRISATQFPDLHERLQRCAGRLGVKLPEA
                     FLMTGDGMLNAFATRFLRRYYVVLLSDVVDALEDDPEAINFYIGHELGHIDRKHIANG
                     WWMAPAMLLPLIGAAYRRAQEYTCDQYGLACCADQASAARALAVLAAGTRRWKSLDAA
                     AYVAQCERTGGFWMSLNELASDYPWLCKRMAHLREPMAKLPSRHPLAWLLALFMPRVG
                     AGGPLLGIVMLFALIGVLLAIALPAYQEHVNRAAAQPAFSYGDQITRATGSYILEHSQ
                     IPGDLGGLGIAAPGSGVQGSIDAESAIITLTLKGEQTITYTPTLDDEGDLYWSCETTL
                     AASAVPAGTACASLAATGVEQDGGWAGLLGRLR"
     gene            complement(2516..>2953)
                     /locus_tag="PLN02_07680"
     CDS             complement(2516..>2953)
                     /locus_tag="PLN02_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52241.1"
                     /translation="CVDLLDARDEKKAPLFSRCTWGVVWPASAAPSPQGDALTLAVQP
                     LDTWREMWVFRKGAAGWQLDVLPPALDNPDLGYIEFAGWVPGGQQLLAARELRAGQQY
                     RTSFETVSLSTLLTDRQADRPSSLTPFYRWQSPQWKAGTVAVR"
BASE COUNT          572 a          991 c          961 g          429 t
ORIGIN      
        1 gagcgccttc ctggccaaca tgagccacga aatccgcacc ccgctcaacg ccatcaccgg
       61 catggcgcac ctgatccggc gcggcggcct caatccggag cagagcgaac gcctggacaa
      121 actggaagcc gccggcaacc atctgctcga catcatcaac gccgtgctcg acctgtcgaa
      181 gatcgaagcc ggcaagctga cgctcgagga aatcccggtc aatatcggca gcatcctcag
      241 caacatcgtc tcgatgctgc acagccgcgc cgaagaccgc ggcatcacgc tgatcgtcga
      301 acccggcgcg ccagcgggca agctgctcgg cgacccgacg cgcctgcagc aagccctgct
      361 caattacgcc gccaacgcca tcaagttcac cagcgaaggc tgggtccggc tggccgccga
      421 gaccgttgcc agcgaaggtg acagcgcgtt gatccgtttc accgtcaccg acaccggcgt
      481 cggcattgaa ccgcaggcgc agtcccgcct cttctcggcc ttcgagcagg ccgacaattc
      541 aaccacccgc aaattcggcg gcactggact ggggctggcg atcacccgca agctcgccga
      601 atcgatgggc ggcgaggtcg gcgtcatcag cacgccaggc gaaggtagct gcttctggtt
      661 caccgcgcgc ctgcggcgcg atcccgacga cagaagccaa acggaaacgg ccagctccgc
      721 ccgcccgacc gacagtctgg cgggccggcg cgtactgctc gctgaagacg aaccggtcaa
      781 tcgcgaaatc gcccggatgc tgctcgacga gatcggcctt gactcggcct gcgccgagga
      841 cggcagcatc gcgctgcaga tggctgcggc ggagcattac gacctgatcc tgatggacat
      901 gcagatgccg aacatggacg gcctcgaggc cacccggcgc atccgcctgt tgcccggcta
      961 cgccggggtg ccgatcctgg cgatgacggc aaatgctttt gccgaagaca aggcgcgctg
     1021 tttcgaagcc ggcatgaacg acttcatcgc caaaccggcc cgccccgaag acctcttcgc
     1081 caagctgcag caatggctgc agtagtctga aaaaaaaagg ccgccggctg agccggcggc
     1141 ctttccaata tcgctcagcg atttcagcgc agacgaccca gcaggccagc ccagccacca
     1201 tcctgctcaa cgccggtcgc cgccaggctc gcgcacgcgg taccggcggg aaccgcgcta
     1261 gccgccagcg tcgtctcgca actccagtag aggtcgcctt cgtcgtccag cgtcggcgtg
     1321 taggtgatcg tctgctcgcc cttcagggtc agggtgatga tcgccgactc ggcgtcgatg
     1381 ctgccctgca cgccgctgcc cggcgcggcg atgccgaggc cgccgagatc gcccggaatc
     1441 tggctgtgtt cgaggatgta gctgccggtc gcccgggtga tctggtcgcc atagctgaac
     1501 gccggctgcg ccgcggcacg attgacgtgt tcctggtagg cgggaagggc gatggcgaga
     1561 agcacgccga tcagcgcgaa gagcatgacg atgccgagca gcggaccgcc ggcgccgacg
     1621 cgcggcatga acagcgccag cagccaggcc agcggatgac gcgacggcaa cttggccatc
     1681 ggttcgcgca agtgcgccat gcgcttgcac agccagggat agtcgctggc cagttcgttg
     1741 agcgacatcc agaagccgcc ggtgcgctcg cactgggcaa cgtaggcggc cgcgtcgagg
     1801 ctcttccagc gccgggtgcc ggcggcgagg acggccaggg cgcgcgccgc cgacgcctga
     1861 tcggcgcagc aggcgaggcc gtactggtcg caggtgtatt cctgggcgcg acggtaggcg
     1921 gcaccgatca gcggcagcag catcgccggc gccatccacc agccattggc gatgtgcttg
     1981 cggtcgatgt ggccgagttc atgaccgata tagaagttga tcgcttccgg gtcatcttcc
     2041 agcgcgtcga ccacgtcgga cagcaggacc acgtagtaac gacgcaggaa gcgggtggca
     2101 aaggcattga gcatgccgtc gccggtcatc aggaaggctt ccggcagctt gacgccgaga
     2161 cgcccggcgc agcgctgcag acgctcgtgc agatcgggaa actgcgtcgc actgatacgc
     2221 acggcgttgc cctgcaggta ggaaatcagc gccgaatggg caaacagcgc gaacaggtac
     2281 atcagcagca tgtacagcca gacgatgccg atggtgccga cggtgagcaa cagccagaac
     2341 agcccggcaa acactgccag gatcgcgaac agggttttct cgtgacggta agcggcgacc
     2401 ggggtcaccg cgagcgaacc gatcagggaa ggagaagtca tggggaactc cggaaacgac
     2461 caaaagggcg gcattatttc acgacatatg aatattcgcc agtcatcatg acggctcagc
     2521 gcaccgccac agtccctgcc ttccactgcg gactctgcca gcggtagaac ggcgtcaggc
     2581 tggacggccg gtcggcctgc cggtcggtga gcagggtgct cagggaaacg gtctcgaagc
     2641 tggtccggta ttgctgcccg gcgcgcagtt cgcgcgccgc cagcagttgc tggccgccgg
     2701 gcacccagcc ggcgaactcg atataaccga ggtcggggtt gtcgagcgcc ggcggcagca
     2761 cgtccagttg ccagcccgcc gcccccttgc ggaacaccca catctcgcgc caggtatcga
     2821 gcggctgcac cgccagggtc agcgcgtcgc cctgcggcga cggggcggcc gacgccggcc
     2881 agaccacgcc ccaggtgcag cggctgaaca aaggggcttt cttttcgtcc ctggcatcaa
     2941 gcagatcgac gca
//
LOCUS       DAOSDX010000307         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11812_length_2953_cov_2.892340, whole genome shotgun sequence.
ACCESSION   DAOSDX010000307 DAOSDX010000000
VERSION     DAOSDX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11812_length_2953_cov_2.892340"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2953
                     /locus_tag="PLN02_07685"
     CDS             <1..>2953
                     /locus_tag="PLN02_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52242.1"
                     /translation="VSLVASEIERSVGERINILEALAGRLTRLPADPAAGQALLDTQT
                     GLDPLFNWGIMVLDAGGTAIASVPARHERSGTHYGDRDFFRELVATGKPLITEPLTGR
                     RTGVPVITIAVPIFSPDKRLIGAVLGITNMGKPNFLDQISQAKYGRTGDFFVTDTRAR
                     MFIASSDKQRVLKKGPPPGVNAVYDSYINGRQGSGVAVSSRGVEELSSSVQIGNTPWV
                     MQSVLPTAEAFAVVREMQQRLLIAGLTFTLFAGAFAWWWVRRQLRPLERSAVLLDEMR
                     QGSRPRSALPIESDDEIGKLANAFNGLLKAIVDQEALLAKVAATELLRKTLAHVPGMV
                     FQYYQHADGSGAFPFASAAVQDIYGVSAEVIEADAHLIRDLLVPEDHDRLFDSMRRSA
                     ANMERWLVDYRIRMADGRIKWLHVDAMPEHEDGRIVWYGFVTDVTATKALEEELEIHR
                     SHLEELVRERTEQLEEARRVAESASLAKSSFLANMSHEIRTPMNAIIGLTGLLERNID
                     DPQHCDKLAKIHLAADHLLAIINDVLDISKIEAGKIQLEDIEFDLTETVTRAGDLIAG
                     KAREKGLDFALELPPPIGGCLRGDPTRLAQALLNYLGNAIKFTEHGGITLRASLLEQT
                     TGTASYRFEVEDSGIGIPTLALPSLFGAFEQADRSTTRQYGGTGLGLAITRQLARMMG
                     GDAGVDSIEGQGSRFWFTARFVRSSRELGGPSARRRSDAPEAVLRRDYAGSRVLLCED
                     NPVNQEVALALLQDVGMRVSIAQNGREAVERFTSQPFALVLMDMQMPLMDGLEATRRI
                     RALPGGETVPILAMTANAFTEDMQRCQAAGMNDFVAKPVEPELLFAKLLQWLPPVPAS
                     PPTTPANTANDGALREALAALPGFDFNAALARLRGNPQRLLKLLHLFVAEHHADPAAL
                     RQALSEGNRQTAEKICHALKGAAGSVGLERIHEPAAVLNALLRQDAADANEAPLIEQI
                     ADALQQLASALE"
BASE COUNT          536 a         1062 c          913 g          442 t
ORIGIN      
        1 gtttcgttgg tcgcttcgga aatcgagcgt tcggtcggcg agcggatcaa catccttgag
       61 gcgctggccg ggcgtctgac caggctgccg gccgatcccg ccgctggaca ggcgctgctc
      121 gatacgcaaa ccgggctcga ccccttgttc aactggggca tcatggtgct cgacgcagga
      181 ggcacggcaa tcgccagtgt tccggcgcgt cacgagcgca gcggcaccca ctatggcgac
      241 cgcgattttt tccgggagct ggtcgccact ggcaagccgc tgatcaccga gccactgacc
      301 ggacgccgca ccggcgtacc ggtaatcacc atcgctgtgc cgattttttc gccggacaag
      361 cggttgattg gcgccgtact cggcatcacc aacatgggga aacccaattt ccttgatcag
      421 atcagccagg ccaaatacgg acgcaccggc gatttcttcg tcactgacac ccgggcccgc
      481 atgttcatcg cctcgtcgga caagcagcgg gttctgaaga agggcccccc acccggcgtc
      541 aacgcggttt acgacagcta catcaacggc cgtcagggat cgggcgtcgc cgtcagttcg
      601 cgcggggtag aggaattgtc gtcgtcggtg cagatcggca ataccccctg ggtcatgcag
      661 tcggtgctgc ccacagccga ggccttcgcc gtcgtgcgcg agatgcagca gcggctgctg
      721 attgccgggc tgaccttcac cctgtttgcc ggggccttcg cctggtggtg ggtccggcgc
      781 cagttgcggc cgctcgaacg ctccgccgtc ctgctcgacg aaatgcgcca gggcagccgg
      841 ccgcgcagcg cgctgccgat cgaatccgac gacgagatcg gcaaactcgc caacgccttc
      901 aacggcttgc tcaaggccat cgtcgaccag gaagccctgc tcgccaaagt agcagccacc
      961 gaattgctgc gcaagacgct ggcccacgtg ccgggcatgg tcttccagta ctaccagcac
     1021 gccgacggca gcggcgcctt tcccttcgcc agcgcggcgg tgcaggacat ctacggggtc
     1081 agcgccgaag tcatcgaggc cgatgctcac ctgatccgcg acctgctggt gccggaagac
     1141 catgatcgcc tgttcgactc gatgcgccgg tcggcggcaa acatggagcg ctggctggtc
     1201 gattaccgga tccgcatggc tgacggccgg atcaagtggc tgcatgtgga cgcgatgccc
     1261 gagcacgagg acggcaggat cgtctggtac ggcttcgtca ccgacgttac cgcgaccaag
     1321 gcgctggagg aggaactgga aatccaccgc agccacctgg aagagctggt ccgcgagcgc
     1381 accgagcaac tggaggaggc gcgacgcgtc gccgaatcgg ccagtctggc gaagagcagc
     1441 ttcctcgcca acatgagcca cgagatccgc accccgatga acgccatcat tggtctcacc
     1501 ggcctgctcg agcgcaacat cgacgatccc caacattgcg acaagctggc caagatccat
     1561 ctggcggcgg atcacctgct ggcgatcatc aacgacgtgc tcgacatctc gaagatcgaa
     1621 gccggcaaga tccagctcga ggacatcgaa ttcgacctca ccgaaaccgt cacccgcgcc
     1681 ggcgatctga tcgccggcaa ggcccgggaa aaagggctgg atttcgccct cgaactgccg
     1741 ccgccgatcg gcggctgcct gcgcggcgat ccgacccggc tggcgcaggc gctgctcaac
     1801 tatctgggca acgcgatcaa gttcaccgaa cacggcggca tcacgctgcg cgccagcctg
     1861 ctcgagcaga cgacgggcac cgccagctac cgcttcgaag tcgaagacag cggcatcggc
     1921 atcccgacgc tggcgctacc cagcctgttc ggcgctttcg agcaggccga ccgctcgacc
     1981 acccggcagt acggcggcac cggcctcggg ctggcgatca cccgccagct agcccgcatg
     2041 atgggcggcg acgccggcgt cgacagcatt gagggacaag gcagccgctt ctggttcacc
     2101 gcccggtttg tccgctcgtc gcgcgaactc ggcgggccca gcgcccgccg ccgcagcgac
     2161 gcccccgaag ccgtcctgcg ccgcgactac gcgggcagcc gcgtcctgct ctgcgaggac
     2221 aacccggtca accaggaagt cgcgctcgcc ctgctgcagg atgtcggcat gcgcgtcagc
     2281 atcgcccaga acggccgcga agccgtcgaa cgtttcacca gccagccctt tgcgctggtc
     2341 ctaatggaca tgcagatgcc gctcatggac ggcctcgaag ccacccgccg catccgcgcc
     2401 ctgcccggcg gcgagacggt gccgatcctg gcgatgacgg ccaacgcctt caccgaggac
     2461 atgcagcgct gccaggccgc cggcatgaac gatttcgtcg ccaagccggt cgagcccgaa
     2521 ttgctcttcg ccaagctgct gcaatggctg ccaccagtcc cggccagccc gccgaccact
     2581 cccgcgaaca ccgccaacga cggcgccctg cgcgaagcgc tggccgccct gcccggcttc
     2641 gacttcaacg ccgcgctggc ccgcctgcgc ggcaacccgc aacgcctgct caagctactc
     2701 cacctgttcg tcgccgaaca ccacgccgac cccgcagccc tgcgccaggc cttgagcgaa
     2761 ggcaaccgcc agacggcgga gaaaatctgc cacgccctca agggagcggc cggcagcgtc
     2821 ggcctcgagc gcatccacga accggccgcc gtactcaacg ccctgctgcg ccaggatgcc
     2881 gccgacgcca acgaggcgcc gctgatcgag cagattgccg atgccctgca gcagctcgcc
     2941 agcgccctcg aag
//
LOCUS       DAOSDX010000308         2952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11814_length_2952_cov_2.565067, whole genome shotgun sequence.
ACCESSION   DAOSDX010000308 DAOSDX010000000
VERSION     DAOSDX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11814_length_2952_cov_2.565067"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLN02_07690"
     CDS             <1..189
                     /locus_tag="PLN02_07690"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOI52243.1"
                     /translation="DARGAISVTERAAYIGRIRNLARAVAQAYLESRARLGFPMAPKA
                     WAAEVLAKLEEQNAKKAA"
     gene            191..2305
                     /gene="glyS"
                     /locus_tag="PLN02_07695"
     CDS             191..2305
                     /gene="glyS"
                     /locus_tag="PLN02_07695"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOI52244.1"
                     /translation="MTVKNLLVELFVEELPPKALKTLGEVFADKLFNHLVKLQLTSPG
                     SVVGLKAFASPRRLAAFVPDVLTQAEDRQVSQKLMPVAVGLDAAGNATPALLKKLAAL
                     GADESAVASLRRESDGKAEILFYDSLAKGATLAEGLQKAIEAALAALPIPKVMSYQLA
                     DGWSSVNFVRPAHALVALHGSDVVPVSVLGLQSGKATKGHRFEAAVDPVVIADADSYA
                     ETLKRDGAVIASFAERRAEIARQLEAAAAQAGGKPIDDDALLDEVTALVERPNVLVGQ
                     FEEEFLAVPQECLILTMKANQKYFPLLDAAGKLTNKFLVVSNISPADPSAVIGGNERV
                     VRPRLADAKFFFDQDRKKSLESRVIGLSKVVYHNKLGTQGERVQRVAAIARAIAEALG
                     GGELVQHAEQAALLAKADLLTDMVGEFPELQGIMGRYYAQHDGLNADVADAIEDHYKP
                     RFAGDSLPRGKVGTVVALADKLETLVGMFGIGQIPTGDRDPFALRRHALGVIRMLAEG
                     DLNLAVDGLLNGVFAQFNGIEGFAPNPQALADFIYDRLAGSLREQGYTAQEVDAVVSQ
                     RPQRLGDIVKRLAAVRAFSALPEAAALAAANKRVGNILKKADGAVEARVDAALLREAA
                     ESALHAALVDVVPQADAAFLTGDYSESLLALAALRAPVDAFFDGVMVNADDPALKANR
                     LGLLAMLHAAMNRVADLSRLSA"
     gene            2312..2872
                     /gene="gmhB"
                     /locus_tag="PLN02_07700"
     CDS             2312..2872
                     /gene="gmhB"
                     /locus_tag="PLN02_07700"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HOI52245.1"
                     /translation="MVAKLVILDRDGVINFDSAQFIKSPEEWKPIPGSLEAIARLKQH
                     GYRVVVATNQSGVGRGLFDMDTLNRIHRKMHKAACELGGRIDAIFYCPHTADSKCDCR
                     KPKPGMFRRIAETLNTELKGVAAIGDSLRDLQAGAAAGCQPILVLTGKGERTREEGDL
                     PAGTLIFANLAAATDHLLAQEGKEKK"
BASE COUNT          528 a         1061 c          918 g          445 t
ORIGIN      
        1 gacgcgcgcg gcgccatctc ggtcaccgaa cgcgccgcct acatcggccg catccgcaac
       61 ctggcccgcg ccgtcgccca ggcctacctc gaatcgcgcg cccgcctcgg cttcccgatg
      121 gcgcccaagg cctgggccgc cgaagtgctg gccaagctcg aagaacagaa cgccaagaaa
      181 gccgcctgag atgactgtca agaacctcct cgttgaattg ttcgttgaag aactgccgcc
      241 caaggcactg aaaacacttg gtgaagtctt cgccgacaaa ctatttaatc acttggtgaa
      301 attacaacta acctcaccgg gttccgttgt cggcctgaaa gcgtttgcca gcccgaggcg
      361 tcttgccgca tttgttcctg acgtacttac acaggctgag gaccgacagg tttcacagaa
      421 gctgatgcca gtcgccgtcg gtctcgatgc cgccggtaac gccacgcccg cgctactgaa
      481 gaagctcgcc gcactcggcg ctgatgaaag cgccgtcgcc agcctgcgcc gcgaaagcga
      541 cggcaaggcc gagatccttt tctacgacag cctggccaag ggcgccacgc tggccgaggg
      601 cctgcagaag gccatcgaag ccgctctcgc cgcgctgccg atccccaagg tgatgagcta
      661 ccagctggcc gacggctgga gcagcgtcaa tttcgtccgc ccggcgcacg cgctggtcgc
      721 cctgcacggc agcgatgtcg tcccggtttc cgtgctcggc ctgcaatcgg gcaaggccac
      781 caagggccat cgctttgaag cggcggtcga tccggtggtg atcgccgatg ccgacagcta
      841 tgccgaaacg ctcaagcgcg acggcgccgt gatcgcctcc ttcgccgagc gccgcgccga
      901 aatcgcccgt cagctcgaag ccgctgccgc ccaggccggc ggcaagccga ttgacgacga
      961 cgccctgctc gacgaggtca ccgcgctggt cgaacgcccg aacgtgctgg tcggccagtt
     1021 cgaggaggaa ttcctcgccg tgccgcagga atgcctgatc ctgaccatga aggcgaacca
     1081 gaaatacttc ccgctgctcg acgccgccgg caagctgacc aacaagttcc tcgtcgtcag
     1141 caacatcagc ccagccgacc cgagcgcggt gatcggcggc aacgagcgcg tcgtgcgccc
     1201 gcgcctggcc gacgccaagt tcttcttcga ccaggaccgc aagaagtcgc tcgaatcgcg
     1261 cgtcatcggc ctgtccaagg tcgtctatca caacaagctg ggcacccagg gcgagcgcgt
     1321 ccagcgcgtc gcggcgattg cccgcgccat cgccgaagcg ctcggcggcg gcgagctggt
     1381 gcagcacgcc gagcaggcag cgctgctcgc caaggccgac ctgctcaccg acatggtcgg
     1441 cgagttcccc gagctgcaag gcatcatggg gcgctactac gcccagcacg acggcctgaa
     1501 tgccgacgtg gcggatgcca tcgaagacca ctacaagccg cgcttcgccg gcgacagcct
     1561 gccgcgcggc aaggtcggca cggtcgtcgc gctggccgac aagctggaaa ccctggtcgg
     1621 catgttcggc atcggccaga tcccgaccgg cgaccgcgac ccgttcgcgc tgcgccgcca
     1681 cgcgctgggc gtcatccgca tgctcgccga gggcgacctc aacctcgccg tcgacggcct
     1741 gctcaacggc gttttcgcgc agttcaacgg catagagggc ttcgcaccga acccgcaggc
     1801 gctggccgac ttcatctacg accgcctcgc cggcagcctg cgcgagcagg gctacacggc
     1861 gcaggaagtc gatgccgtgg tcagccagcg gccgcagcgc ctgggcgaca tcgtcaagcg
     1921 cctggccgct gtgcgcgcct tctcggcgct gcccgaagcc gccgcgctgg ccgccgccaa
     1981 caagcgggtc ggcaacatcc tgaagaaggc cgacggcgcc gtcgaagccc gcgtcgatgc
     2041 ggcgctgctg cgcgaagccg ccgaaagcgc gctgcacgcg gcgctggtcg acgtcgtgcc
     2101 gcaggccgat gccgccttcc tcaccggcga ctacagcgaa tcgctgctcg ccctggcggc
     2161 gctgcgcgca ccggtcgatg ccttcttcga cggcgtcatg gtcaatgccg acgatccggc
     2221 gctcaaggcc aaccggcttg gcctgctcgc catgctgcac gcggcgatga accgcgtcgc
     2281 cgacctttcc cggctgtcgg cgtaaatggc gatggtggcc aagctcgtca tcctcgaccg
     2341 cgacggggtg atcaatttcg actccgcgca gttcatcaag agccccgagg aatggaagcc
     2401 gatccccggc agcctggagg cgatcgcccg cctcaagcag cacggctacc gggtggtcgt
     2461 cgccaccaac cagtcgggcg tcggtcgcgg cctgttcgac atggatacgc tcaaccgcat
     2521 ccatcgcaag atgcacaagg ccgcctgcga gctcggcggg cgcatcgacg ccatcttcta
     2581 ttgcccgcac accgccgatt cgaaatgcga ctgccgcaag ccgaaaccgg gcatgttccg
     2641 gcgcatcgcc gaaaccctca acaccgagct caagggcgtc gccgccatcg gcgattcgct
     2701 gcgtgacctg caggccggcg ccgccgccgg ctgccagccg atcctggtgc tcaccggcaa
     2761 gggcgaaaga acccgcgagg aaggtgatct gcccgccggc acactgattt tcgccaacct
     2821 cgccgcggcg accgatcacc tgctggccca ggaaggcaag gaaaagaaat gagcgcagtc
     2881 cgttccaccc tgttcatgat ctacgcgatc ctctggtcga tgctcagcgc gccgctggtg
     2941 gtgatcgccg gg
//
LOCUS       DAOSDX010000309         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11832_length_2948_cov_3.024888, whole genome shotgun sequence.
ACCESSION   DAOSDX010000309 DAOSDX010000000
VERSION     DAOSDX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11832_length_2948_cov_3.024888"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1643
                     /locus_tag="PLN02_07705"
     CDS             15..1643
                     /locus_tag="PLN02_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HOI52246.1"
                     /translation="MPAAAQWRVEAPDGLAGRISPFLTEHTGRQGARALGEEILATEG
                     YFTPQIEIADGDAGLTLKIDPGPRTRIAEVDLAIAGPLDPARREALLAGWRLPVGQPF
                     RQADWNDAKQQVLAELLAVEHAAARLDDSAATIDAATQRATLRAHYDAGPRYRYGELR
                     INGLHRFPPELVARYNRQVRAGRPYRAEDLHLLASQLQATPYFASARAELDLDAASVD
                     ADGNATVPVDLRLRERPGHRAGFGAGFSSNTGARVEANYQTPNFLNQAWEFTGGLRLE
                     QKRQTLYGDVFLPPDASQRRHGFGFAQETSDIEDLRTERHAFGVQSIQQRGSVEQRLS
                     LNWQRERRSPDGADSVTSRALVANATWTWRAVDNPLDPRAGLVLQLSLGGAARALLSD
                     ADFVRAHGRVQYYLPVGSRDTLALRGELGRTYADSRLHVPQDYLFRTGGTGSVRGYAY
                     QSLGIREGTATVGGRYMAVASAEYTHWLNDDWGVAAFIDAGDAVDALADARLAVGYGL
                     GVRWRSPAGPIGADLGYGERERAVQLHVSLAIPF"
     gene            1646..>2948
                     /locus_tag="PLN02_07710"
     CDS             1646..>2948
                     /locus_tag="PLN02_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52247.1"
                     /translation="MKRRLAALALLIALPLAGLVWLTGTGSGLRTLTGLASTASGGRL
                     QFAGVDGRLAGPLQIAEVQWQDGEQHITIGQLQLAWTPAALIDGRLQIDALTIGELRI
                     DSATATTPTPLPADLRLPLALSVDRLEIAAVHYNGSAVANDLRARLASDGRQHRLSAL
                     SLRSGDVVIAGDGEIDGLAPFPLRADLAVHGQLAERPLALDLHAAGPLAQLPLRLQAK
                     SGIAGDGEALLTPFAAQPFASARIDLSDIDPTAWQAALPSARLAARIDIAPTDDGVSA
                     RFRIANSQPGPLDRQALPFAELAGDASWQAGRLQLPALHLTLAGGGELQGRGDWQGEQ
                     DGSLHLELTASRLDAGRLLAALRPTRLAGTIKAELGASAQQVRVDLTDPRFRLRAAVE
                     HAAATVRVEQLELAAGDARLQASGELHTGDLSFRADARLERF"
BASE COUNT          426 a         1211 c          955 g          356 t
ORIGIN      
        1 tcctctgctg cgcgctgccc gccgccgcgc agtggcgggt cgaagcgccc gacgggctgg
       61 ccggccgcat ctcgcccttc ctcaccgaac acaccgggcg ccagggggct cgcgcgctgg
      121 gcgaggaaat cctcgccacc gaaggttatt tcacgccgca gatcgaaatc gccgacggcg
      181 atgccgggct gacgctgaag atcgaccccg gcccgcgcac ccgcatcgcc gaagtcgacc
      241 tcgccatcgc cggcccgctc gacccggcgc gccgcgaggc gctgcttgcc ggctggcggc
      301 tgccggtcgg ccagcccttc cgccaggccg actggaacga cgccaagcag caggtgctgg
      361 ccgaactgct cgccgtcgaa cacgccgccg cccgcctcga cgacagcgcg gcgacgatcg
      421 acgcagcgac gcagcgggcg acgctgcgcg cccattacga cgccggcccg cgctaccgct
      481 acggcgaact gcgcatcaat ggcctgcacc gcttcccgcc cgaactggtc gcccgctaca
      541 accgccaggt gcgggccggg cgaccttacc gcgccgagga cctgcacctg ctcgccagcc
      601 agttgcaggc gacgccctac ttcgccagcg cccgcgccga actcgacctg gatgccgcca
      661 gtgtcgacgc cgacggcaac gcgacggtcc cggtcgacct acggctgcgc gaacgccccg
      721 gtcaccgcgc cggtttcggc gccggtttca gttcgaacac cggcgcccgc gtcgaggcca
      781 actaccagac cccgaacttc ctcaaccagg cctgggagtt taccggcggc ctgcgtctcg
      841 aacagaagcg gcagaccctc tacggcgacg tcttcctgcc gccggacgcc agccagcgcc
      901 ggcacggttt cggcttcgcg caggaaacca gcgacatcga ggacctgcgc accgaacgcc
      961 acgccttcgg cgtgcagagc atccagcagc gcggcagcgt cgaacagcgg ctgtcgctca
     1021 actggcagcg cgagcggcgc tcgccggacg gcgccgacag cgtcaccagc cgggcactgg
     1081 tcgccaacgc cacgtggacc tggcgcgccg tcgacaaccc gctcgacccg cgcgccggcc
     1141 tggtgctcca gttgagcctc ggcggcgccg cccgcgccct gctttccgac gccgacttcg
     1201 tgcgcgccca cggccgcgtc cagtactacc tgcccgtcgg cagccgcgac accctggcgc
     1261 tgcgcggcga actcggccgc acctacgccg acagccgcct gcacgtgccg caggactacc
     1321 tgttccgcac cggcggcacc ggctcggtgc gcggctacgc ctaccagagc ctcggcatcc
     1381 gcgaaggcac ggcgaccgtc ggcggccgct acatggccgt cgccagcgcc gagtacacgc
     1441 actggctgaa cgacgactgg ggcgtcgccg ccttcatcga cgccggcgac gccgtcgatg
     1501 ccctcgccga cgcccgcctc gccgtcggct acgggctcgg cgtgcgctgg cgcagcccgg
     1561 ccgggccgat cggcgccgac ctcggctacg gcgaacgcga gcgcgccgtg caactgcatg
     1621 tctcgctggc aatcccgttc tgatcatgaa acgccgcctt gccgcccttg ccttgcttat
     1681 tgccctgcca ctggccggcc tcgtctggct gaccggcacc ggcagcggcc tgcgcacgct
     1741 gaccggcctc gccagcacgg cgagcggcgg tcgcctgcag ttcgccggcg tcgacggccg
     1801 ccttgccggc ccgctgcaga tcgccgaagt gcaatggcag gatggcgagc agcacatcac
     1861 catcggccaa ctgcagctcg cctggacgcc ggccgcgctg atcgacggcc ggctgcagat
     1921 cgacgcactg acgatcggtg aactgcgcat cgacagcgct accgcgacca cgccgacgcc
     1981 gcttcccgcc gacctgcgcc tgccgctggc gctaagcgtc gaccggctgg agatcgccgc
     2041 cgtgcattac aacggtagcg ccgtcgccaa cgacctgcgc gcccgcctgg ccagcgacgg
     2101 ccgccagcat cgcctgagcg cgctcagcct gcgcagcggc gacgtcgtca tcgccggcga
     2161 cggcgaaatc gacggcctcg cccccttccc cctgcgcgcc gacctcgccg tgcacggcca
     2221 actggccgag cgcccgctcg ccctcgacct tcacgccgcc ggcccgctcg cccaactgcc
     2281 gctgcgcctg caggcgaaaa gcggcatcgc cggcgacggc gaagcgctgc tgacgccgtt
     2341 cgccgcccaa cctttcgcca gcgcccgcat cgacctcagc gacatcgacc cgaccgcctg
     2401 gcaggccgcg ctgcccagcg cccggctggc cgcccgcatc gatattgcgc cgaccgacga
     2461 cggggtgagc gcccgcttcc gcatcgccaa cagccagccc ggcccgctcg accgccaggc
     2521 gctgccgttc gccgaactgg ccggcgatgc cagctggcag gccggccgcc tgcaactgcc
     2581 ggcactgcac ctgacgctcg ccggcggcgg cgaactgcaa gggcgcggcg actggcaggg
     2641 cgagcaggac ggcagcctgc acctcgaact gaccgccagc cgcctcgacg ccggccgcct
     2701 gctcgccgca ttgcggccga cccggctggc cgggacgatc aaggccgaac tgggcgccag
     2761 cgcccagcag gtccgcgtcg acctgaccga cccgcgcttt cgcctgcgcg ccgcggtcga
     2821 gcacgcggcg gcgacggtgc gcgtcgaaca actcgaactg gcggccggcg acgcccgcct
     2881 gcaggcaagc ggcgaactgc acaccggcga cctcagcttc cgcgccgacg cccgactcga
     2941 gcgcttcg
//
LOCUS       DAOSDX010000310         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11861_length_2944_cov_3.010730, whole genome shotgun sequence.
ACCESSION   DAOSDX010000310 DAOSDX010000000
VERSION     DAOSDX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11861_length_2944_cov_3.010730"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1698)
                     /gene="nifL"
                     /locus_tag="PLN02_07715"
     CDS             complement(166..1698)
                     /gene="nifL"
                     /locus_tag="PLN02_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation negative regulator NifL"
                     /protein_id="HOI52248.1"
                     /translation="MPAQTQPETPSLQDLKDLLPPDVYRQAVSQADLAISITDPKARI
                     LYANDSFSRITGYSAEEIVGLNESVLSNHTTPRELYQEMWTALQAHQPWSGKLLNKRK
                     CGGTYLAEVSITPVHNAAGEITHFLGMHRDVTELHRLERSLRNHKHLIESVVDAAPVA
                     FALLDPAGRVVLDNQEYKKLVTDMRAKEPAHRLLDALAPEWYEALAGDIRRCEFTNRE
                     MRLDSPVGRARWLSVSASAIEMHNDCADSYFCAAGQPGLLLVISDITNLREEQERAKG
                     AALKASLADEERTAAIREGLSAAVFRLEEPLNVMASAIAVLQRRDPGSAEMLRHVLET
                     SREHVESLRQVIPQSPQEMVVRVNLNEILRDVLEVCTARLLKAGITVDWQPSAVLPPL
                     PGRPLQLRMLFKALVENAIEAMNVKGWPRRELQIKSEVIDDCIRVSVLDSGPGIPMEW
                     RHKAFEPFFTAKNGSGKHIGTGLSRAQQVVADHGGIIDLDESPSGGCAAIVEFRVDGD
                     PI"
     gene            1972..2550
                     /gene="rsxA"
                     /locus_tag="PLN02_07720"
     CDS             1972..2550
                     /gene="rsxA"
                     /locus_tag="PLN02_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOI52249.1"
                     /translation="MSEILLLLLSTALVNNVVLIKFLGLCPVMGVSKSVDSAFGMGLA
                     TTFVLVLASAASWMLDNWLLQPLGLGYLRILCFILVIASVVQFTEMFIKKSSPGLYQS
                     LGIYLPLITTNCAVLGVALLNVQQQFSLFKSLLYGFGSALGFTLVLLIFAGLRERVAL
                     ARVPAAFAGAPVAFVTISLLALAFMGFSGLNV"
     gene            2565..>2944
                     /locus_tag="PLN02_07725"
     CDS             2565..>2944
                     /locus_tag="PLN02_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOI52250.1"
                     /translation="MIAAILSLTLLGAALGVVLGLANRVLKVEGNPVVEELIAMMPGS
                     NCGQCGFPGCPGAAEAIVAGTAPPTCCPPGGKALASAIAAKLGITVDLSALGDDGPKI
                     AVVAEELCIGCCRCSKVCPTDAIIG"
BASE COUNT          428 a          931 c          994 g          591 t
ORIGIN      
        1 gcagcgattc gcggaagttg agcgaccggc tcagttcccg gctcaccgca tagagcgcgg
       61 aaaggagcag gatgttgagt tcgtgcgtgc ggcagaagcc gccttccggg gggcagtcgt
      121 cggcgttgtg gatggtgtgg tgttcctgca tgctcatggt cctgatcaga tcgggtcgcc
      181 gtcgacgcgg aattcgacga tggcggcgca gccgccgctc gggctctcgt cgagatcgat
      241 gatgccgccg tggtcggcga ccacctgctg ggcgcgcgac aggccggtgc cgatatgctt
      301 gccgctgccg ttcttggccg tgaagaaggg ttcgaaagcc ttgtgccgcc actccatcgg
      361 gatgccgggg ccgctgtcga ggaccgagac gcggatgcag tcgtcgatca cctcgctctt
      421 gatctgcaac tcgcggcgcg gccagccctt gacgttcatc gcttcgatcg cgttctcgac
      481 cagcgccttg aacagcatgc gcagttgcag cggtcgcccg ggcagcggcg gcagcactgc
      541 cgacggctgc cagtcgacgg tgatgccggc cttgagcagg cgcgccgtgc acacttcgag
      601 gacgtcgcgc aggatttcgt tgagattgac ccgcaccacc atttcctgcg ggctctgcgg
      661 gatcacctgg cgcagcgatt cgacgtgttc gcggctggtt tccagcacat ggcgcagcat
      721 ctcggcgctg cccgggtcgc gccgctgcag cacggcgatc gccgacgcca tcacgttgag
      781 cggttcctcg aggcggaaga cggcggccga caggccctcg cggatcgccg ccgtgcgttc
      841 ctcgtcggcc agcgaggcct tcaacgcggc gcccttggcg cgctcctgtt cctcgcgcag
      901 gttggtgatg tcggaaatca ccagcagcag gccgggctgg ccggcggcgc agaaatagct
      961 gtcggcgcag tcgttgtgca tctcgatcgc cgaggcgctg accgacagcc agcgggcgcg
     1021 gccgaccggg ctgtcgaggc gcatctcgcg gttggtgaat tcgcagcgcc ggatgtcgcc
     1081 ggccagcgcc tcgtaccatt ccggcgccag cgcatcgagc aggcggtgcg ccggttcctt
     1141 ggcgcgcatg tcggtgacca gcttcttgta ttcctggttg tcgaggacga cgcggccggc
     1201 cgggtcgagc agggcgaagg cgaccggtgc cgcatcgacc accgattcga tcaggtgctt
     1261 gtggttgcgt agcgaacgct caagccggtg tagttcggtg acgtcgcggt gcatgccgag
     1321 gaagtgggtg atttcgccag cggcgttgtg caccggcgtg atcgacactt cagcaaggta
     1381 ggtgccgccg cacttgcgct tgttgagcag cttgcccgac cacggctggt gcgcctgcag
     1441 cgcggtccac atttcctggt agagctcgcg cggcgtggtg tggttggaaa gcaccgattc
     1501 gttgaggccg acgatttcct cggcgctgta gccggtgatg cggctgaagc tgtcgttggc
     1561 gtaaaggatg cgtgccttcg ggtcggtgat cgagatcgcc agatcggcct ggctgaccgc
     1621 ctggcgatag acgtcgggcg gcaggagatc cttcaggtcc tgaaggctgg gcgtctcggg
     1681 ttgagtctgg gccggcatgg ggtactccgc aggggcgtca aaaacgatgc ggtgtttaaa
     1741 gcaggtttca tgccgcaccc tgcggatttt ctgaaatgca cggtttggcg gggcttgggc
     1801 gggaattcga caattttcct gtggactttg cgacaaagtg ttgcggggaa aacgcaccac
     1861 tttggtgtgc gctctggaaa atgggcacgg aatggcccgc ccagggcctt ggcgacggat
     1921 ggcacggctt atgcagtact ggcggtgatc ttcctttcga gacttccgtc catgagcgaa
     1981 atcctcttat tgctgctctc caccgcgctg gtcaataacg tggtgctgat caagttcctc
     2041 ggcctctgcc cggtgatggg cgtctcgaag agcgtggaca gcgccttcgg catgggcctg
     2101 gcgacgacct tcgtcctggt gctcgccagc gccgcttcgt ggatgctcga caactggttg
     2161 ctgcagccgc ttggcctcgg ctacctgcgc atcctctgct tcatcctggt gatcgcctcg
     2221 gtcgtgcaat tcaccgagat gttcatcaag aagtcgagtc ctggcctcta ccagtcgctc
     2281 ggcatctacc tgccgctgat caccaccaac tgcgccgtcc ttggcgtcgc gctgctcaac
     2341 gtgcagcagc agttcagcct gttcaagagc ctgctctacg gcttcggttc ggcgctcggc
     2401 ttcacgctgg tgctgctcat cttcgccggc ctgcgcgaac gcgtcgcgct ggcccgggta
     2461 cccgccgcct tcgccggggc gccggtcgcc ttcgtcacga tcagcctgct ggcccttgcc
     2521 ttcatgggct tttccggcct caacgtctga aaaggaggaa tccgatgatc gccgccatcc
     2581 tcagcctgac cctgctcggc gccgcgctcg gtgtcgtgct cggccttgcc aaccgcgtcc
     2641 tcaaggtcga gggcaatccc gtcgtcgaag aactgatcgc catgatgccc ggctcgaact
     2701 gcggccagtg cggcttcccg ggttgccccg gggcggccga ggcgatcgtc gccggcacgg
     2761 cgccgccgac ctgctgcccg cccggcggca aggcgctagc ttcggcgatt gccgccaagc
     2821 tcggcatcac cgtcgatctt tcggcgctcg gcgacgacgg tccgaagatc gccgttgtcg
     2881 cagaagagct gtgcatcggc tgctgccgtt gttccaaggt gtgcccgacc gacgccatca
     2941 tcgg
//
LOCUS       DAOSDX010000311         2937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11895_length_2937_cov_3.352186, whole genome shotgun sequence.
ACCESSION   DAOSDX010000311 DAOSDX010000000
VERSION     DAOSDX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11895_length_2937_cov_3.352186"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /gene="creD"
                     /locus_tag="PLN02_07730"
     CDS             complement(<1..1192)
                     /gene="creD"
                     /locus_tag="PLN02_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HOI52251.1"
                     /translation="MDRKLLFKIIAVVVMSILLLVPLALIEGQIRDRSQRQHEVQQNI
                     ANSSAGAQTLVGPVILLEFRERVQEPTRDPASGKITGTVSRIETQRQLISAESLNIDG
                     SARVENRHRGIYQARLFHLDLANQAKLVIEPFAASAPERTLLDAEATLVLGVADPRGV
                     DVDPEVTINGKPFRFVTPGNRLETGEALKMPQLIVPLGRIDPSKTHSFDVRFPLQLTG
                     TTRLAITPSADSNRIHLKSDWPHPNFGGRFLPRERSVGADGFDAQWAISHLARDFDQA
                     LDPTSGEALSIDFVDPVNVYLQSERAVKYGILFIVLTFAGFFLTEILRRAPIHPMQYL
                     LVGLALALFFLLLIALSEHLSFALAYGISSLACIALIGFYLAGALAGARRGLAFAGGL
                     TGWYG"
     gene            1429..1776
                     /locus_tag="PLN02_07735"
     CDS             1429..1776
                     /locus_tag="PLN02_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52252.1"
                     /translation="MWTALAELFVARELQDYDYRSIAQTLRAAGIGAAELGNILRDEV
                     APAFMGNFSGLNPMPEMEGWSENEVRERVRAAMARSRGWGSRLFRRLRPDPLRHPVLR
                     ERCRALLELLRII"
     gene            complement(2009..2293)
                     /locus_tag="PLN02_07740"
     CDS             complement(2009..2293)
                     /locus_tag="PLN02_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52253.1"
                     /translation="MPKWIYLLGASLFSAIFGEAVFFTIIDPQQLYLFGEPVSWQPVA
                     VYSAGFFMFWGLTFFNAALVTWMQKPGDEVNREPQEQARRLEQRNRLKST"
     gene            2401..2556
                     /locus_tag="PLN02_07745"
     CDS             2401..2556
                     /locus_tag="PLN02_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52254.1"
                     /translation="MTQQNPLYLSSQSICFGGDFHAGRLLWALLIVEIDPVTNDLASM
                     LKGLVRT"
BASE COUNT          589 a          869 c          924 g          555 t
ORIGIN      
        1 ccccatacca gccggtgagg ccgccggcaa aggccagccc gcggcgcgcg ccggccagcg
       61 cgccagccag gtagaagccg atcagcgcga tgcaggcgag cgaggaaatg ccgtaagcca
      121 gcgcgaacga caggtgttcg gacaaggcga tgagcagcag gaagaagagc gccagcgcca
      181 gcccgaccag caggtactgc atcggatgga tcggcgcgcg gcgcaggatt tcggtgagaa
      241 agaagccggc aaaggtgagg acgatgaaca ggatgccgta cttcaccgct cgctccgatt
      301 gcaggtaaac attcaccgga tcgacgaagt cgatcgacag cgcctcgccc gaagtcgggt
      361 ccagcgcctg gtcgaaatcg cgggcgaggt gggaaatcgc ccactgcgca tcgaagccgt
      421 cggcgccgac gctgcgctcg cgcggcagga aacggccgcc gaaattgggg tgcggccagt
      481 ccgacttcag gtgaatccgg ttgctgtcgg cgctcggcgt gatcgccagg cgggtggtgc
      541 cggtcagttg cagcggaaag cgcacgtcga aactgtgcgt cttgctcggg tcgatgcgcc
      601 ccagcggcac gatcagttgc ggcatcttca gcgcttcacc ggtttccagc cggttgcccg
      661 gcgtgacgaa acggaaaggc ttgccattga tcgttacctc gggatcgaca tcgaccccgc
      721 gcggatcggc cacgccgagc accagcgtcg cctcggcatc caacagcgtg cgctccggcg
      781 cgctggcggc gaacggctcg atcaccagct tcgcctggtt ggccagatcg agatggaaca
      841 gccgcgcctg gtaaatgccg cgatggcggt tctccacccg cgccgaaccg tcgatgttca
      901 ggctctcggc ggagatcaac tggcgctgcg tctcgatgcg cgacaccgtg ccggtgatct
      961 tgccgctggc cggatcgcgc gtcggttcct gcacccgctc gcggaattcg agcaggatca
     1021 ccggcccgac cagcgtctgt gcgccggccg aactgttggc aatgttctgc tgcacctcgt
     1081 gctggcgctg actacgatcc cgaatctgcc cctcgatcag cgccagcggc accagcagca
     1141 ggatggacat gacgacaacg gcaataatct tgaacagcaa cttgcggtcc atgaaaggct
     1201 ccctgagtga atgacggagc cagtctcgcc gccgcaagtg agggcagggc gaagtcgggg
     1261 cgggggattg tgaagcgggg gtgaagtggg cggtcgacca ccgcaagccg cagcattcca
     1321 tggcgcggga gtaagccaat ggaatgctat gatcccgccc acttgccctc cctggctgtg
     1381 cgctgaacga tggctccacc cgcttccgaa cccgaaaacc gtgtccaggt gtggactgcc
     1441 ctggccgaac ttttcgtcgc gcgcgaattg caggattacg actatcgaag catcgcgcaa
     1501 acgttgcgcg cagcagggat cggtgcggcg gagctgggga acatcctgcg cgacgaggtg
     1561 gcgccagcct tcatgggcaa cttcagtggg ctcaacccga tgcctgaaat ggaaggctgg
     1621 tccgagaatg aagttcggga aagggtgcgc gccgccatgg cgcgttctcg gggttggggg
     1681 tcgcggctgt tcaggcgctt gcgcccggac ccgctacggc atccggtgct gcgggagcgc
     1741 tgccgggctc ttctggagtt gcttcggata atttgagccc ggcccctttt tctttcggcc
     1801 ctttttttcc cgggattcac ttgccgcaac caggtgaggc gagcttgcaa ttaggcgcgg
     1861 aaaaccgggt gctgatcgcg gttctgtcct ccggggccgt gtggcggcga cagttggtga
     1921 ggatagcttt cggccggtcg tgttccgagc aaagacctgc accaaactga agtgcaaatc
     1981 gcggcaaggg cggggtgtac cccgttcgct aggtggattt caggcggttt ctttgctcaa
     2041 gacggcgtgc ctgttcttgc ggctcacgat tgacttcatc gcccggtttt tgcatccagg
     2101 tgaccagggc cgcgttgaag aaagtcaaac cccagaacat gaagaatccg gccgaataga
     2161 ccgcaaccgg ttgccaggac accggttcgc caaacaagta gagctgttgc ggatcgatga
     2221 tcgtgaaaaa gaccgcttca ccgaaaatgg cgctgaacaa agaagcacca agtaagtaga
     2281 tccatttcgg catactgacc tcccgagcca tcaaggtgtg acaaaaggtg ttgcacgctg
     2341 tacttggacc atgccgggcc gtaaagttca attccggccg gtattccgac ggcaagtgta
     2401 ttgacccagc agaacccgct ctacttatcc agccaaagca tatgcttcgg cggggatttt
     2461 catgccgggc gcctgttgtg ggcgcttttg attgtggaaa ttgatccagt caccaatgat
     2521 ctggctagca tgctgaaggg tctggtcaga acctgagttc agccagccaa tgcagcagaa
     2581 atttgcatgt tgcgttcatg caacacctgt tgcatccatg ccgaaacatc accttgaccc
     2641 ctaccccatc cccccgcgat acttccccca cgttttgcca accgcattta ttgaaaggag
     2701 tacgacatgc ctgcactaga tcacgaggtt ttccagcgct gagccgcagg cgagtcgtcg
     2761 cccggaacgc gctgatcagc gaatccgggc cgggatatga accctggccg tccgccgggg
     2821 ttttttttat gcctttttgg cggttgaccg caaagttttc attgcgggcc ggattccata
     2881 gcaaagcact attctggaat ccatcatggg cattcaatta gaactgaaca aggggcg
//
LOCUS       DAOSDX010000312         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11918_length_2934_cov_2.613755, whole genome shotgun sequence.
ACCESSION   DAOSDX010000312 DAOSDX010000000
VERSION     DAOSDX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11918_length_2934_cov_2.613755"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLN02_07750"
     CDS             <1..530
                     /locus_tag="PLN02_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI52255.1"
                     /translation="LDTPSVGEYYLAGENVATLDKDALAVLRNRTLGFVFQGFNLLPR
                     MSLQDNVALPLVYAGVDKATRRARARALLERVGLGKYAESLPNRISGGQQQRVAIARA
                     LVNAPRLILADEPTGNLDSHTSEEIMRLFGELNGDGITIVLVTHEPDIAAHARRQVRF
                     LDGRIVSDHLTGADA"
     gene            527..1747
                     /locus_tag="PLN02_07755"
     CDS             527..1747
                     /locus_tag="PLN02_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI52256.1"
                     /translation="MIHPMLGEAWLAMGANRLRTALTMLGMVIGVGAVVIMLAIGQGA
                     QYAVQQTISTMGANLFIVLSGSFTTSGVRSGSGGGPTLSVADAEAIAELDGVVNVAPT
                     HQGTRQLVYGAQNWSSQVIGTTPAYLDARAWSVANGGAFGDSDVRSATRVALIGKTVA
                     ENLFGDEDPVGKTLRIQQSPFVVVGVLGAKGQNLDGRDQDDSVIIPLSTAQRKVFGTP
                     FLGSVRMIMVQADSADNMERVMDATTALLRQRHRLRDDMPDDFFMRNLSAAAESEAAT
                     TRTMSVLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARRKDILSQFLLE
                     SVMISFAGCLLGLLLGVGIALAINAFTGMVVVISGSAALLAFAVAAGVGIFFGWYPAQ
                     KAARLDPIEALRYQ"
     gene            complement(1749..2246)
                     /locus_tag="PLN02_07760"
     CDS             complement(1749..2246)
                     /locus_tag="PLN02_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOI52257.1"
                     /translation="MRLWHALRHLSAPSWLLRRAVGPAELDALTASIAASERQHRGEI
                     CVAVDSTLPWSALRRDLDSRQRAAELFQQYGVARTREATGILIYVQLLDRRLEILADR
                     GIAACVAQAEWDAICRALATEFAAGRYANGLHLAVDRATTLLAQHFPAGDDNPDELAN
                     RPRLR"
     gene            complement(2248..>2934)
                     /locus_tag="PLN02_07765"
     CDS             complement(2248..>2934)
                     /locus_tag="PLN02_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOI52258.1"
                     /translation="SALNERLARLETEKGAQVVVLIVPTTQPESIEQYGIRLAEAWKI
                     GRRGVNDGVIVIVARDDRAMRIEVGYGLEGAIPDAIARRIIDEQMAPRFRQGDFAGGL
                     NAAVDALGRLIGGEALPPPAAKPGDKGSSFDSDTLLFVAIFVASIARAIFGLLGSLAV
                     SLLAGWLAWMSFGSLGTGLIAIVVTFLASFLRVGRGGNWRGGGGGFSGGGGFSGGGGS
                     FGGGGASGRW"
BASE COUNT          464 a         1011 c         1016 g          443 t
ORIGIN      
        1 gcctcgacac gccgagcgtc ggcgagtatt acctggccgg cgagaacgtc gcgacgctgg
       61 acaaggacgc gctcgccgtc ctgcgcaacc ggacgctcgg tttcgtcttc cagggcttca
      121 acctgctgcc gcgcatgagc ctgcaggaca atgtcgcgct gccgctggtc tatgccggcg
      181 tcgacaaggc aacccggcgg gcccgggcgc gtgccctgct cgaacgcgtc ggcctcggca
      241 agtacgccga gtcgctgccc aaccggattt ccggcggcca gcagcagcgg gtggcgatcg
      301 cccgcgcgct ggtcaacgcg ccgcggctga tcctcgccga cgagccgacc ggcaacctcg
      361 acagccacac cagcgaggaa atcatgcgcc tgttcggcga actcaacggc gacggcatca
      421 ccatcgtcct ggtcacccac gaacccgaca tcgcggcgca cgccagacgc caggtgcgtt
      481 tcctcgacgg ccgcatcgtc agcgaccacc tcaccggagc cgacgcatga tccatccgat
      541 gctcggcgaa gcctggctgg cgatgggcgc caaccgtctg cgcacggcgc tgaccatgct
      601 cggcatggtg atcggcgtcg gcgccgtggt gatcatgctg gccatcggcc agggcgcgca
      661 gtacgcggtg cagcagacga tcagcacgat gggcgccaac ctgttcatcg tcctttccgg
      721 ctccttcacc acctccggcg tgcgtagcgg cagcggcggc gggccgacgc tgagcgtcgc
      781 cgacgccgag gcgatcgccg aactcgacgg cgtcgtcaac gtcgcgccga cccaccaggg
      841 aacgcggcaa ctggtctatg gcgcgcagaa ctggagcagc caggtgatcg gcacgacgcc
      901 cgcctacctc gacgcgcgcg cctggagcgt cgccaatggc ggcgccttcg gcgattccga
      961 cgtgcgctcg gcgacgcggg tggcgctgat cggcaagacc gttgccgaaa acctgttcgg
     1021 cgacgaggac ccggtcggca agacgctgcg catccagcag agccccttcg tcgtcgtcgg
     1081 cgtgcttggc gccaaggggc agaacctcga cggccgcgat caggacgaca gcgtgatcat
     1141 tccgctcagc accgcccagc gcaaggtctt cggcacgccc ttcctgggct cggtgcggat
     1201 gatcatggtg caggccgact cggccgacaa catggaacgg gtgatggacg cgacgaccgc
     1261 gctgctgcgc cagcgccacc gcctgcgcga cgacatgccc gacgacttct tcatgcgcaa
     1321 cctgtcggcg gcggccgagt cggaagcggc gacgacgcgg acgatgtcgg tcctgctcgg
     1381 ggcgatcgcc tcggtctcgt tgctcgtcgg cggcatcggc atcatgaaca tcatgctcgt
     1441 ctcggttacc gagcgaacgc gcgagatcgg catccggatg gcgatcggcg cccggcgcaa
     1501 ggacattctc agccagttcc tgctcgaatc ggtgatgatt tccttcgccg gctgcctgct
     1561 cggcctgctg ctcggcgtcg gcatcgcgct ggcgatcaac gccttcaccg gcatggtcgt
     1621 cgtcatttcc ggcagcgcag ccctgctcgc cttcgcggtg gcggccgggg tcggcatctt
     1681 cttcggctgg tacccggcgc agaaggcagc gcgcctcgac ccgatcgagg cgctgcgcta
     1741 ccagtaattc agcgcaggcg cggccggttg gcgagttcgt ccgggttgtc gtcgccggcc
     1801 ggaaagtgct gcgcgagcag cgtcgtcgcc cggtcgacgg cgaggtgcag gccgttggcg
     1861 tagcggccgg cggcgaactc ggttgccaaa gcgcggcaga tggcgtccca ttcggcctgc
     1921 gcgacgcagg cggcgatgcc gcggtcggcg aggatttcca ggcggcggtc gagcaattgg
     1981 acgtagatca ggataccggt cgcctcgcgg gttcgagcga cgccgtactg ctggaacagt
     2041 tctgcggcgc gctgccggct gtcgaggtcg cggcgcagcg ccgaccaggg cagggtgctg
     2101 tcgacagcga cacagatttc gccgcgatgc tgccgttcgg aagcggcgat gctcgccgtc
     2161 agggcgtcga gttcggccgg gccgacggcg cgccgcagta gccaggacgg tgcgctcaga
     2221 tggcgcaggg catgccagag tcgcatttca ccacctcccc gaggcgccgc cgccaccgaa
     2281 actgccacca ccgcccgaga atccgccacc gccggaaaaa ccgccaccgc cgccgcgcca
     2341 gttgccgccg cgcccgacgc gcaggaaaga ggcaaggaag gtgacgacga tggcgatcag
     2401 gccggtgccg agcgaaccga agctcatcca ggccagccag ccggcgagca gcgagaccgc
     2461 cagcgaaccg agcagaccga agatggcgcg ggcgatgctg gcaacgaaga tggcgacgaa
     2521 gagcagggtg tcgctgtcga aactgctgcc cttgtcgcct ggtttggcgg ctggcggcgg
     2581 cagtgcttcg ccgccgatca ggcggcccag cgcatcgacc gcggcgttga ggccgccggc
     2641 gaagtcgccc tggcggaaac gcggcgccat ctgttcgtcg atgatgcggc gggcgatggc
     2701 gtcgggaatt gcgccttcca gcccgtaacc gacctcgatg cgcatcgccc ggtcgtcgcg
     2761 ggcgacgatg acgatgacgc cgtcgttaac gccgcggcgg ccgatcttcc aggcttcggc
     2821 gaggcggatt ccgtattgct cgatgctttc cggttgcgtc gtcggcacaa tcaggacgac
     2881 gacctgggcg cccttttcgg tttccaggcg ggccaggcgt tcgttcagcg cgct
//
LOCUS       DAOSDX010000313         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11947_length_2928_cov_3.847894, whole genome shotgun sequence.
ACCESSION   DAOSDX010000313 DAOSDX010000000
VERSION     DAOSDX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11947_length_2928_cov_3.847894"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLN02_07770"
     CDS             <1..801
                     /locus_tag="PLN02_07770"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOI52259.1"
                     /translation="RLGERIAALEARQEASRDQEAALQALFQDVASGREAADLLEIER
                     DVTLAAQQLQLAGDVPVALLALRAADAQLARLDKASLLPLRKALAADIARLDGLPRVD
                     LAGTSLRLEEVLRGIDKFPFAAHARPREETPAVAADAAPALPWWRLALADAWQEIRNL
                     IRIQRFDRAEAPLLAPEQGFFLRENLKLRLLSARLALFARDEASFRNELKAAQDWLGR
                     YFAGDDRAVAAALAQLKELAAADIVLELPDLRASQQALAALREAGEKQ"
     gene            798..1985
                     /locus_tag="PLN02_07775"
     CDS             798..1985
                     /locus_tag="PLN02_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HOI52260.1"
                     /translation="MKGLFWLLLLFALAVVVALAASLNAGYVLLVVPPWRVEVSLNFF
                     VLALLIGFFVAYAALRAVSLTLGMPAQVGAYRRRQLREKSGQMFQDAVRHLFEGRFGQ
                     ALKRAGEAYDAGRAPALAALIAARAAQRLGEPGRQQEWLEKAKQQDARNEAATLMLEA
                     EMYNEERRFDEALAALQRLQGKQGRHIAALRLELRARQGLGDWDGVLKLLRQLAKRDA
                     LAPELVSALSSKAHLANLARCGADREALVAYLRKLPEAERSLKVVLAAVRGLAAAGAD
                     GDAQQLIEEQLEGNDEESWQSELPAIYGSLAGGDQLSRIARGEAWLRQRPAHAALLLA
                     LGRMCARQRLWGKAQSFLEASLAVEDTQAGHLELARLDDQLERVDDANRHYRAAVAPN
                     TLK"
     gene            complement(2002..2898)
                     /gene="hemF"
                     /locus_tag="PLN02_07780"
     CDS             complement(2002..2898)
                     /gene="hemF"
                     /locus_tag="PLN02_07780"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HOI52261.1"
                     /translation="MSSPQLKEFFTSLQSSIVAELEAFDGKPFLTDTWQRPEGGGGIS
                     RLIEEGDFFERGGVNFSHVTGHALPASATAVRPQLAGRAWEAMGVSLVLHPRNPYCPT
                     AHMNVRCFVARKEGEDDVWWFGGGMDMTPYYGQREDVAHFHRSCRDALAPFGDDVYAK
                     YKQWCDEYFFLKHRNEPRGVGGVFFDDLNEGGFERCFALTQAVGQAFTRAYLPVLAKR
                     RATPYGERERDFQAYRRGRYVEFNLVWDRGTLFGLQSGGRTEAILMSLPPVVKWRYDW
                     QPAPGTPEAELYEVFLKPQDWV"
BASE COUNT          440 a         1009 c         1043 g          436 t
ORIGIN      
        1 cggctgggcg agcgcatcgc cgccctcgaa gcgcgccagg aagccagccg cgaccaggaa
       61 gccgccctcc aggcgctgtt ccaggacgtc gccagcggcc gcgaggcggc cgacctgctc
      121 gagatcgagc gcgacgtgac gctggccgcg cagcaactgc aactggccgg cgacgtgccg
      181 gtcgcgctgc tcgccctgcg tgccgccgac gcccagctcg cccgcctcga caaggccagc
      241 ctgctgccgc tgcgcaaggc gctggcggcc gacatcgccc gtctcgacgg cctgccgcgc
      301 gtcgacctcg ccggcaccag cctgcgtctc gaggaagtct tgcgcggcat cgacaagttc
      361 cccttcgccg cgcatgctcg accgcgcgag gaaaccccgg cggtcgccgc cgacgcggcg
      421 ccggccttgc cctggtggcg tctggcgctg gccgatgcct ggcaggaaat ccgcaacctg
      481 atccgcatcc agcgtttcga tcgcgccgaa gcgccgctgc tggcgcccga gcagggcttc
      541 ttcctgcgcg aaaacctcaa gctgcgcctg ctcagcgccc gccttgcgct gttcgcccgc
      601 gatgaggcga gtttccgcaa cgaactgaag gcggcgcagg actggctggg ccgctatttc
      661 gccggcgacg accgcgcggt ggcggcggcc ctggcgcagt tgaaggaact cgccgccgcc
      721 gacatcgtcc tcgaactgcc cgatctgcgg gccagccagc aggcgctggc ggccttgcgc
      781 gaagccgggg aaaaacagtg aagggcttgt tctggctgct cctgctgttc gccctggcgg
      841 tggtcgtcgc gcttgccgcc agcctcaatg ccggttacgt gctgctcgtc gtgccgccct
      901 ggcgggtcga ggtatcgctc aacttcttcg tgctggcgtt gttgattggc ttcttcgtcg
      961 cctacgccgc cctgcgcgcc gtctcgctga ccctgggcat gccggcgcag gtcggcgcct
     1021 atcgccggcg ccagttgcgc gagaagtccg ggcagatgtt ccaggatgcc gtgcgccatc
     1081 tcttcgaagg ccgtttcggc caggccctga agcgcgccgg cgaagcctac gatgccggcc
     1141 gggcgccggc gctggcggcg ctgatcgccg cccgtgcggc gcagcgcctg ggcgagccgg
     1201 ggcgccagca ggaatggctg gaaaaggcca aacagcagga cgcgcgcaac gaagcggcga
     1261 cgctgatgct ggaagccgag atgtacaacg aggagcgccg tttcgatgaa gcgctggccg
     1321 cgctgcagcg cctgcagggc aagcagggcc ggcacatcgc cgcgctgcgc ctggaactgc
     1381 gggcgcgcca ggggctcggc gactgggacg gcgtgctcaa gctgctgcgc cagttggcca
     1441 agcgcgacgc gttggccccg gaactggtca gcgcgctgtc gagcaaggcg catctggcca
     1501 atcttgcccg ttgcggcgcc gaccgcgagg cgctggtcgc ctacctgcgc aagctgccgg
     1561 aggcggagcg cagcctcaag gtcgtcctcg ccgccgtgcg cggcctggcg gcggcgggag
     1621 cggacggcga cgcgcagcaa ctgatcgagg aacaactgga aggcaacgac gaggagtcct
     1681 ggcagagcga gctgccggcg atctacggca gcctcgccgg cggcgaccag ttgtcgcgca
     1741 tcgcccgcgg cgaagcctgg ctgcggcagc ggccggcgca cgccgcgctg ctcctggcac
     1801 tgggccggat gtgcgcgcgc cagcggctgt ggggcaaggc gcagagtttc ctggaagcgt
     1861 cgctggcggt cgaggatacg caggccggcc accttgaact ggcccgtctc gacgaccagc
     1921 tcgaacgcgt cgacgacgcc aaccgccact accgcgcggc ggtcgctccg aacacgctga
     1981 agtgagtcgg ccgctgccgg ctcagaccca gtcctgcggc ttgaggaaga cctcgtagag
     2041 ttccgcttcc ggcgtacccg gcgccggctg ccagtcgtag cgccatttga cgaccggcgg
     2101 cagcgacatc aggatcgcct cggtacggcc gcccgactgc aggccgaata gcgtgccgcg
     2161 gtcccagacc aggttgaact cgacgtagcg gccgcgccgg taggcctgga aatcgcgttc
     2221 gcgctcgccg tagggcgtgg cgcggcgctt cgccagcacc ggcaggtagg cgcgggtgaa
     2281 ggcctggccg accgcctggg tcagtgcgaa gcagcgctcg aacccgccct cgttgaggtc
     2341 gtcgaaaaag acgccgccga cgccgcgcgg ctcgttgcgg tgcttgagga agaaatactc
     2401 gtcgcaccac tgcttgtact tcgcgtagac gtcgtcgccg aagggcgcca gcgcgtcgcg
     2461 gcagctacgg tggaaatgcg cgacgtcctc gcgctgcccg taatagggcg tcatgtccat
     2521 gccgccgccg aaccaccaga cgtcgtcctc gccttccttt ctggcgacga agcagcgcac
     2581 gttcatgtgc gccgtcgggc agtaggggtt gcgcggatgc agcaccagcg agacgcccat
     2641 cgcctcccag gcgcggccgg ccagttgcgg gcggacggcg gtggcggaag cgggcagcgc
     2701 gtggccggtg acgtgcgaga aattgacccc gccgcgctcg aagaagtcgc cttcctcaat
     2761 caggcgggaa atcccgccgc cgccttcggg acgctgccag gtatcggtca ggaagggttt
     2821 gccgtcgaag gcttcgagtt cggcgacgat gctcgactgc agcgaggtga agaattcttt
     2881 gagttgcgga ctggacatgg gtttctggag atgttcggga aacgactt
//
LOCUS       DAOSDX010000314         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11951_length_2928_cov_2.556213, whole genome shotgun sequence.
ACCESSION   DAOSDX010000314 DAOSDX010000000
VERSION     DAOSDX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11951_length_2928_cov_2.556213"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLN02_07785"
     CDS             <1..941
                     /locus_tag="PLN02_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOI52262.1"
                     /translation="IEQLKAVAGQAGVDFFPSAVGEKPEAIAVAAIDWAKRHYHDVLL
                     VDTAGRLAIDEEMMAEIKRLHAAIKPIETLFVVDAMLGQDAVNTARAFNEALPLTGVV
                     LTKLDGDARGGAALSVRHITGKPIKFAGVGEKLTGLEAFHPERMASRILGMGDVLSLI
                     EEAKKGIDEEKAVAFAKKLKAGKGFDLNDFKEQIGQMRAMGGLTSLMDKLPAQFAQAA
                     GQLPAGAENKMVGRVEGIINSMTPQERAKPELIKASRKRRIAAGAGVQVQEVNRLLNQ
                     FEQTQKMMKQFSKGGIGKLMRGMKGMMPGGMGGFGR"
     gene            complement(942..1553)
                     /locus_tag="PLN02_07790"
     CDS             complement(942..1553)
                     /locus_tag="PLN02_07790"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOI52263.1"
                     /translation="MRRLIGIAWLCCASAVNAGAQKYEPLAASVRAALHEAVSDSRPQ
                     VSSFKSPLEATNWLTEMSGRLARRIPDRNYRIDLLRSIHYEATRAGLDPHLVLGLIQV
                     ESAFRKYAVSTVGARGYMQVMPFWLKVIGRPEDNLFDMRTNLRYGCTILRHYLDIEKG
                     DLYRALGRYNGSLGKPEYPNLVRGAWQSNWSYASPAGLAANAR"
     gene            complement(1541..>2928)
                     /locus_tag="PLN02_07795"
     CDS             complement(1541..>2928)
                     /locus_tag="PLN02_07795"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOI52264.1"
                     /translation="DIARAEIHSWRQLPKNFYHIQTKFRDEIRPRFGVMRGREFLMKD
                     GYSFHTSFDDLVREYGNMYATYSRIFTRLGLKFRAVAADTGAIGGDGSHEFHVLADSG
                     EDDIAFCPDSDYAANVELAEAVAPATPRAAAGKTMEKIATPGKMACADVAAFLAIEPT
                     QIVKSIAVVAEREDGDKQFALLLLRADHELNEIKAAKIPTLGAFRFASDSEVQEYLGC
                     VPGYIGPVGVDAGKVTVFADRTVAAMSDFVCGANEAGFHLSGVNFGRDLPEPAVFDIR
                     NVVAGDPSPDGKGVLGICRGIEVGHIFQLRSKYAKALNCAFLDENGKSQIMEMGCYGI
                     GVSRIVGAAIEQGNDDKGIILPPTIAPFAVCIVPMGYHKSEAVKAAADQLYADLKKLG
                     IDVVLDDRNERPGVMFADMELIGIPHRVVIGERGLKDGQFEYKGRTEAEATMIAQGEI
                     VGLLQGKLCGA"
BASE COUNT          498 a          868 c         1035 g          527 t
ORIGIN      
        1 caatcgagca gttgaaggcg gtggccggtc aggccggggt ggatttcttc ccgtcggcgg
       61 tcggcgagaa gccggaagcg atcgctgtcg cggcgatcga ttgggcgaag cgccactatc
      121 acgacgtgct gctggtcgat accgccgggc gtctcgccat cgacgaagag atgatggcgg
      181 aaatcaagcg cctgcacgcg gcgatcaagc cgatcgaaac gctgttcgtg gtcgatgcca
      241 tgctcggcca ggatgcagtc aatacggcgc gcgccttcaa cgaggcgttg ccgctgaccg
      301 gtgtcgtgct gaccaagctt gacggcgatg cgcgtggcgg tgccgcgctg tcggtacgcc
      361 acatcaccgg caagccgatc aagtttgctg gggtcggcga gaagctcacc ggcctcgaag
      421 ctttccatcc cgagcggatg gcgtcgcgta tcctcggcat gggcgacgtc ctgtcgctga
      481 tcgaagaggc caagaaaggc atcgacgaag agaaggcggt ggctttcgcc aagaagctca
      541 aggccggcaa gggctttgat ctcaacgact tcaaggagca gatcggccag atgcgggcga
      601 tgggtggcct gacttcgctg atggacaagt tgccggccca gttcgcgcag gctgccggtc
      661 agttgccggc gggggcggaa aacaagatgg tcggccgggt cgagggcatc atcaattcga
      721 tgacgccgca ggagcgcgcc aaaccggaac tgatcaaggc cagccgcaag cgccggattg
      781 ctgccggggc cggtgtgcag gtgcaggaag tcaatcgtct gctcaaccag ttcgagcaga
      841 cgcagaagat gatgaagcag ttttccaagg gcggcatcgg caagctgatg cgcggcatga
      901 aaggaatgat gcccggcggt atgggcggtt tcggccgctg atcagcgggc gttggcggcc
      961 agcccggctg ggctggcgta gctccagttg ctctgccagg cgccgcgcac caggttcggg
     1021 tattccggct tgcccaggct gccgttgtag cgcccgaggg cgcggtaaag gtcgcccttc
     1081 tcgatgtcca gataatggcg caggatggtg cagccgtaac gcaggttggt cctcatgtcg
     1141 aagaggttgt cctccggccg gccaatcact ttcagccaga acggcatgac ctgcatgtag
     1201 ccgcgggcac cgacggtgga tacggcgtac ttgcggaagg ccgattcgac ctggatcaga
     1261 ccgaggacca agtgcgggtc gaggccggca cgggttgcct cgtagtggat gctgcgcagc
     1321 aggtcgatcc ggtagttgcg gtcgggaatg cggcgggcca gccgacccga catctcggtc
     1381 aaccagttgg tggcttccag cggcgacttg aacgagctga cctgtggccg gctgtccgag
     1441 accgcttcat gtagcgctgc ccgcacgctg gcggcgagcg gttcatactt ctgcgcaccg
     1501 gcgttgaccg ccgatgcgca gcacagccag gcgatgccga tcaggcgccg cacagttttc
     1561 cttgcagcag gccgacgatc tcgccctggg cgatcatcgt cgcttccgcc tcggtgcggc
     1621 ccttgtactc gaactgaccg tccttcaggc cgcgttcgcc gatcaccacg cggtgcggaa
     1681 tgccgatcag ctccatgtcg gcgaacatga cgccggggcg ctcgttgcgg tcgtccagca
     1741 cgacatcgat gccaagcttt ttcaggtcgg cgtagagctg gtcggcggcg gccttgaccg
     1801 cttcgctctt gtggtaaccc atcggcacga tgcagacggc gaagggggcg atggtcggcg
     1861 gcaggatgat gcccttgtcg tcgttgccct gctcgatggc ggcgccgacg atgcgcgaga
     1921 cgccgatgcc gtagcagccc atctccatga tctggctctt gccgttctcg tcgaggaatg
     1981 cgcagttcag ggccttggcg tacttgctgc gcaactggaa gatgtggccg acttcgatgc
     2041 cgcggcagat gccgagcacg cccttgccgt caggcgaggg gtcgccggcg acgacgttgc
     2101 ggatgtcgaa gactgccggt tcgggcaggt cgcggccgaa gttgacgccg ctgaggtgga
     2161 agccggcctc gttggcgccg cacacgaagt cgctcatcgc ggcgaccgta cggtcggcaa
     2221 agaccgtcac cttgccggcg tcgacgccga ccgggccgat atagcccgga acgcagccga
     2281 gatactcctg gacttcgctg tcagaggcga agcggaaagc gccgagcgtc gggatcttgg
     2341 ctgccttgat ctcgttcagt tcgtggtcgg cgcgcagcag cagcagggcg aactgcttgt
     2401 cgccgtcctc gcgttcggca acgacggcaa tcgacttgac gatctgcgtc ggctcgatcg
     2461 ccaggaaggc ggcgacgtcg gcgcaggcca tcttgcccgg cgtggcgatc ttttccatcg
     2521 tcttgccggc ggcggcacgc ggcgttgccg gggcgacggc ttctgccagt tcgacgttgg
     2581 cggcgtaatc ggagtccggg cagaaggcga tgtcgtcctc gccggagtcg gccaggacgt
     2641 ggaattcatg cgagccatcg ccgccgatgg cgccggtgtc ggccgccacg gcgcggaact
     2701 tcaggccgag ccgggtgaag atgcggctgt aggtggcgta catgttgccg tactcgcgga
     2761 ccaggtcgtc gaacgaggtg tggaaggagt agccgtcctt catcaggaat tcgcggccgc
     2821 gcatgacgcc gaagcgcggg cggatctcgt cgcggaactt ggtctggatg tgatagaaat
     2881 tcttcggcag ttggcgccag ctgtggattt cggcgcgggc gatgtcgg
//
LOCUS       DAOSDX010000315         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_11987_length_2922_cov_3.083014, whole genome shotgun sequence.
ACCESSION   DAOSDX010000315 DAOSDX010000000
VERSION     DAOSDX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11987_length_2922_cov_3.083014"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..760
                     /locus_tag="PLN02_07800"
     CDS             116..760
                     /locus_tag="PLN02_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HOI52265.1"
                     /translation="MFGALMPKEGKYFDHFNAHGALVAQGGSTLRQLIDVLSNGAEEG
                     KAQALADEIDRLESEADKITHEVLAQLHSAFITPFDRDEIHELINGMDNILDMTQDVA
                     EAMALYDIHRIPAEARAMAEITERSCQKVTELVKLLRNMDNGPSILKLCHEIDAYESE
                     VDRMLRVSMSRIFREEPDVREVIKLKEIYQLLESVTDRCKDVAGTVEAIVLENS"
     gene            769..1776
                     /locus_tag="PLN02_07805"
     CDS             769..1776
                     /locus_tag="PLN02_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOI52266.1"
                     /translation="MEAIHMSLGVVVTLVVVALLFDFMNGFHDAANSIATIVSTRVLK
                     PHHAVIWAASFNFLAYFLFHLKVAATIGKGTIDPSIVDHYVIFGALVGAIAWNVITWY
                     YGIPSSSSHALVGGLVGAAVAKAGFGSLVTAGVLKIIAFIFVAPLLGFLIGGLLMVAV
                     SWICRNMAPRKVDKHFRRFQLLSAAAYSLGHGGNDAQKTVGIIWLILIAAGMSTTESE
                     IPGWVVFACYTAMGLGTMFGGWRIVKTMGNRITKLNQARGFCANSGGAVTLFLASAFG
                     IPVSTTHTITGAIAGVGTTRGTRHVRWGVAGGIVWAWILTIPCSALMAALGWYAGRLI
                     L"
     gene            1884..>2922
                     /gene="glnS"
                     /locus_tag="PLN02_07810"
     CDS             1884..>2922
                     /gene="glnS"
                     /locus_tag="PLN02_07810"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOI52267.1"
                     /translation="MSSENKTPAPAANFIKNIVEADLAAGKHAQRRWAGQPGLAAEHA
                     AAPLDAAKIRTRFPPEPNGYLHFGHAKSILLNFGLAQEFGGRCHLRFDDTNPEKEEQE
                     YVDSIVDAVKWLGCSWGDEREDNLYYASNYFDWMVQFAEYLISAGHAYVDSQSAEEMR
                     ANRGTLTEAGKPSPFRARSVAENMDLFKRMQAGEFADGAHILRAKIDMASPNINLRDP
                     AIYRIRHATHHNTGDKWCIYPMYTFAHPIEDALECITDSICTLEFEDQRPFYDWLLER
                     LAEGGLLQRPLPQQIEFSRLNLTYIVLSKRKLIQLVEEKHVSGWDDPRMPTLAGARRR
                     GYTPEGFRRFTD"
BASE COUNT          546 a         1058 c          824 g          494 t
ORIGIN      
        1 atcgtccatg cctgaccggt cggcgggaaa tctcgcgccg gcttgatcca gcgcaacccg
       61 cctccagccc gggtgctaaa atggcgtttt gtcacaaaag ttaaacaaaa tcgtcatgtt
      121 cggcgccttg atgcccaaag agggcaaata tttcgaccac ttcaacgctc acggcgcact
      181 tgtcgcccag ggcggcagca cgctccgcca attgatcgac gtcctgtcca atggtgccga
      241 ggaaggcaag gcacaggccc ttgccgacga gatcgaccgc ctggaaagcg aagccgacaa
      301 gatcacccac gaggtcctgg cccagctgca cagcgccttc atcacgccgt tcgaccgtga
      361 cgagatccac gaactgatca acggcatgga caacatcctc gacatgacgc aggacgttgc
      421 cgaagccatg gcgctctacg acatccaccg cattccggcc gaagcccgcg ccatggccga
      481 gatcaccgag cgtagctgcc agaaggtcac cgaactggtg aagctgttgc gcaacatgga
      541 caacggcccg agcatcctca agctctgcca tgaaatcgac gcatacgaat ccgaagtcga
      601 ccgcatgctg cgcgtctcga tgtcgcgcat cttccgcgag gagccggatg tccgcgaagt
      661 gatcaagctc aaggaaatct accagttgct ggaatcggtg accgaccgct gcaaggatgt
      721 cgccggcacg gtcgaagcca tcgtcctcga aaactcctga gtccgggcat ggaagccatc
      781 cacatgagcc tcggggtcgt cgtcaccctg gtggtcgtgg ccttgctgtt cgatttcatg
      841 aacggctttc acgacgccgc caactcgatc gccaccatcg tctcgacccg cgtcctgaag
      901 ccgcatcacg cggtgatctg ggccgcctcg ttcaacttcc tggcctactt cctgttccac
      961 ctgaaagtcg ccgcgaccat cggcaagggc accatcgacc cgagcatcgt cgaccactac
     1021 gtgatcttcg gtgcgctggt cggcgccatc gcctggaacg tcatcacctg gtactacggc
     1081 attccctcgt cgtcgtcgca cgcgctggtc ggcggcctgg tcggcgccgc cgtcgccaag
     1141 gccggtttcg gcagcctggt cacggccggc gtgctcaaga tcatcgcctt catcttcgtt
     1201 gccccgctgc tcggtttcct catcggcggc ctgctgatgg tggccgtctc gtggatctgc
     1261 cgcaacatgg cgccacgcaa ggtggacaag cacttccgcc gcttccagct gctctccgcc
     1321 gcggcctaca gccttggcca cggcggcaac gacgcgcaga agacggtcgg catcatctgg
     1381 ttgatcctga tagccgccgg catgtcgacc acggaatcgg aaatccccgg ttgggtcgtt
     1441 ttcgcctgct acacggcgat gggcctgggc acgatgttcg gcggctggcg catcgtcaag
     1501 accatgggca accgcatcac caaactgaac caggcacgcg gcttctgtgc caacagcggt
     1561 ggcgccgtca ccctgttcct ggcttccgcc ttcggtatcc cggtgtcgac cacgcacacc
     1621 atcactggcg ccatcgccgg cgtcggcacc acgcgcggca cccgccacgt gcgctggggc
     1681 gtcgccggcg gcatcgtctg ggcgtggatc ctgaccatcc cgtgcagcgc gctgatggcc
     1741 gccctcggct ggtatgctgg ccgcctgatc ctctgatcgg caccgccgta caaacgggcc
     1801 ggccaagcgc cggcccgttt cgttttccgg gagcgcggcg ctgttagaat gtcgctttac
     1861 cgaatcgccc acgccggcta cccatgtcct ccgaaaacaa gactcccgct cccgccgcca
     1921 atttcatcaa gaacatcgtc gaggccgacc tcgccgccgg caaacacgcc cagcgccgct
     1981 gggccggcca gcctggcctc gccgccgagc acgcggcggc gccgctcgat gcggcgaaga
     2041 tccgcacccg cttcccgccc gagccgaacg gctacctgca cttcggccac gccaagtcga
     2101 tcctgctcaa cttcggcctc gcccaggaat tcggcggccg ctgccacctg cgcttcgacg
     2161 acaccaatcc ggaaaaggaa gagcaggaat acgtcgactc catcgtcgac gccgtcaaat
     2221 ggctgggctg cagttggggc gacgagcgcg aggacaacct ctactacgcg tcgaactact
     2281 tcgactggat ggtccagttc gccgaatacc tgatctccgc cggccacgcc tacgtcgata
     2341 gccagagcgc cgaggaaatg cgcgccaacc gcggcaccct gaccgaggcc ggcaagccct
     2401 cgcccttccg cgcgcgcagc gtcgccgaga acatggacct gttcaagcgc atgcaggccg
     2461 gcgaattcgc cgacggcgcc cacatcctgc gcgccaagat cgacatggcc tcgcccaaca
     2521 tcaacctgcg cgacccggcg atctaccgca tccgccacgc cacccaccac aacaccggcg
     2581 acaagtggtg catctacccg atgtacacct tcgcccaccc gatcgaggac gcgctggagt
     2641 gcatcaccga ctccatctgc acgctcgaat tcgaggacca gcgcccgttc tacgactggc
     2701 tgctcgaacg cctcgccgaa ggcggcctgc tgcagcgccc gctgccgcaa cagatcgaat
     2761 tctcgcgtct caacctgacc tacatcgttc tctccaagcg caagctgatc cagctcgtgg
     2821 aagaaaagca cgtctccggc tgggacgacc cgcgcatgcc caccctcgcc ggtgcgcgcc
     2881 gccgcggcta cacgccggaa ggtttccgcc gcttcaccga cc
//
LOCUS       DAOSDX010000316         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12002_length_2920_cov_3.219546, whole genome shotgun sequence.
ACCESSION   DAOSDX010000316 DAOSDX010000000
VERSION     DAOSDX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12002_length_2920_cov_3.219546"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..399
                     /locus_tag="PLN02_07815"
                     /pseudo
     CDS             1..399
                     /locus_tag="PLN02_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            673..1602
                     /gene="cysD"
                     /locus_tag="PLN02_07820"
     CDS             673..1602
                     /gene="cysD"
                     /locus_tag="PLN02_07820"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOI52268.1"
                     /translation="MTQRKTLSHLDWLEAEAIHIMREVAGQCANPVLLFSGGKDSICM
                     LRIAEKAFRPGKFPFPLMHIDTGHNYQEVVAFRDKRAAELGERLIVRSVEDSMKRGTV
                     VLKHEGESRNKHQSVTLLEAIEEFGFDACIGGARRDEEKARAKERIFSFRDEFGQWDP
                     KNQRPELWSLYNARSHKGENIRAFPISNWTEMDVWQYIEREKLELPSIYFAHTRPVVL
                     RQGAIVPVNVPLVSGELTNLPKAGEQIIDKLVRFRTVGDISCTAPVESDADTVEKIVV
                     ETATTTITERGATRLDDQTSEASMEQRKKEGYF"
     gene            1698..>2920
                     /locus_tag="PLN02_07825"
     CDS             1698..>2920
                     /locus_tag="PLN02_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI52269.1"
                     /translation="MSANQTIEDHGLLRFLTCGSVDDGKSTLIGRLLFDTKTILADTL
                     NAIEKTSQKRGLGAVDLSLLTDGLQAEREQGITIDVAYRYFSTGTRKYIIADAPGHEQ
                     YTRNMVTAASTANLAIILIDARKGVLTQTRRHSKLASLVGIPHLVVAINKMDLVDYSQ
                     ETYEKIKADYLEFAAKVGIADVRFIPLSALNGDMIVERGEQLGWYDGPTLMEILEAVP
                     AAHTEHAEKFRFPVQYVCRPQDSANPELHDYRGFMGRVESGSIKVGDQVTVLPNGLES
                     TVKAVQLGGVDIGAAFTEQSVTILLADEIDCSRGDMIVKTAEAPAAVKEIRATVCWLA
                     EAPLDRARTYLIRHTTRDSKAKLTAIDHRLDVNTLENLPADKLAMNDIAEVTFKLAQP
                     LFVDPYTENRGTGAF"
BASE COUNT          634 a         1016 c          835 g          435 t
ORIGIN      
        1 gacatgaccc gcgaggtccg cgacatcgcc gatcagacca acctgcttgc cctcaacgcg
       61 gcgatcgagg cggcgcgtgc cggcgaggcc gggcgcggtt tcgccgtggt cgccgacgaa
      121 gtgcgcaagc tggccgagaa atcgggcaag tcggcggtcg agatcgacca gcggacgcga
      181 gtgatcatgg atcagtcctc ggccgtgcag acggcgatca gcgccggcga ggcatcgatc
      241 cgggccagca acctgctggc cggcgaggtc gaggaggtgc tccagggctc aagccaagtc
      301 gtcgaacgct cgcgcagcgg cgtcaccgaa atcaccgatt cggtcgccga acagaagatc
      361 gcctcgaccg aaatcgccca gaacatggaa cgaatctcga acatggtcga ggaaaacaac
      421 gcctcgacgc aaagcatccg ccaatcgacc gacgacctgc atcgcctgtc ggaacgcctg
      481 agcggcctga ttgccggctt ccgcgtcgcc tgaaacgacg ccccgctgtt tcaacgacga
      541 cgcaggcaca gcggcgacac ctggctcgtt ttattcaaaa acagaataac aagatgcgct
      601 catgttctta attgaatatt aggcgcttgc tacaattcgc tccattcgac aatccctgcg
      661 gattacccag caatgaccca acgcaagaca ctctcccatc tcgactggct cgaagccgaa
      721 gccatccaca tcatgcgcga agtggccggc cagtgcgcca acccggtgct gctcttctcc
      781 ggcggcaagg actcgatctg catgctgcgc atcgccgaga aggccttccg ccccggcaag
      841 ttccccttcc cgctgatgca catcgacacc ggccacaact accaggaagt ggtcgccttc
      901 cgcgacaagc gcgccgccga actgggcgag cgcctgatcg tccgctcggt tgaagactcg
      961 atgaaacgcg gcacggtggt cctgaagcat gaaggcgaat cgcgcaacaa gcaccagtcg
     1021 gtgacgctgc tcgaagccat cgaggaattc ggcttcgacg cctgcatcgg cggcgcccgc
     1081 cgcgatgaag aaaaggcgcg cgccaaggaa cgcatcttca gcttccgcga cgaattcggc
     1141 caatgggacc cgaagaacca gcgcccggaa ctgtggagcc tgtacaacgc ccgcagccac
     1201 aagggcgaga acatccgcgc cttcccgatc tcgaactgga cggaaatgga cgtctggcaa
     1261 tacatcgagc gtgaaaagct ggaactgccg agcatctact tcgcccacac ccgcccggtc
     1321 gtgctgcgcc agggtgccat cgtgccggtc aatgtgccgc tggtttccgg cgagctgacc
     1381 aacctgccga aggccggcga gcagatcatc gacaagctgg tgcgcttccg caccgtcggc
     1441 gacatctcct gcacggcgcc ggtcgagtcg gacgccgaca cggtcgagaa gatcgtcgtc
     1501 gaaaccgcca ccaccaccat caccgaacgc ggcgccacgc gtctggacga ccagaccagc
     1561 gaagcctcca tggaacaacg caagaaagag ggttacttct gacaacacgc cgcgaagcgg
     1621 cttgttgcgg cgtaggctaa cgcggcgtag ccgggttatg ccgaagccaa ctaaccacct
     1681 aaccggatac gaaaacgatg tcagcaaatc aaactattga agatcacggc ctgctgcgct
     1741 ttttgacctg cggcagcgtc gacgacggca agagcaccct gatcgggcgc ctcttgttcg
     1801 acaccaagac catcctcgcc gacaccctga acgccatcga gaagacctcg cagaagcgcg
     1861 gcctcggcgc ggtcgacctc tccttgctca ccgacggcct gcaagccgag cgcgagcaag
     1921 gcatcaccat cgacgtcgcc taccgttatt tcagcaccgg gacgcgcaag tacatcatcg
     1981 ccgacgcgcc gggccacgag cagtacaccc gcaacatggt caccgccgcg tccaccgcca
     2041 acctggccat catcctgatc gatgcgcgca agggcgtgct gacgcagacc cgccgccact
     2101 ccaagctggc gtcgctggtc ggcatcccgc acctggtcgt cgccatcaac aagatggatc
     2161 tggtcgatta ctcgcaggaa acctacgaga agatcaaggc cgactacctc gaattcgccg
     2221 ccaaggtcgg catcgccgac gtgcgcttca tcccgctctc cgcgctcaac ggcgacatga
     2281 tcgtcgagcg cggcgagcag ctcggctggt acgacggccc gaccctgatg gaaatcctcg
     2341 aagccgtgcc ggccgcgcat accgaacacg ccgagaagtt ccgcttcccg gtccagtacg
     2401 tctgccgccc gcaggattcg gccaaccccg agctacacga ctaccgcggc ttcatgggcc
     2461 gcgtcgaatc cggcagcatc aaggtcggcg accaggtcac cgtgctgccc aacgggctcg
     2521 aatcgacggt caaggccgtg caactgggcg gcgtcgatat cggcgcagcc ttcaccgagc
     2581 aatcggtgac gatcctgctg gccgacgaaa tcgactgctc gcgcggcgac atgatcgtca
     2641 agaccgccga agccccggcc gccgtcaagg aaatccgcgc caccgtctgc tggctggccg
     2701 aagccccgct cgaccgcgcc cgcacctacc tgatccgcca caccacgcgc gactccaagg
     2761 ccaagctgac ggccatcgac caccgtctcg acgtgaacac cctggaaaac ctgccggccg
     2821 acaaactggc gatgaacgac atcgccgaag tcactttcaa gctggcgcaa ccgctgttcg
     2881 tcgatcccta caccgagaac cgcggcaccg gcgccttcat
//
LOCUS       DAOSDX010000317         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12040_length_2912_cov_3.732587, whole genome shotgun sequence.
ACCESSION   DAOSDX010000317 DAOSDX010000000
VERSION     DAOSDX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12040_length_2912_cov_3.732587"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLN02_07830"
     CDS             complement(<1..319)
                     /locus_tag="PLN02_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOI52270.1"
                     /translation="MVLIKSQRDTLLAPLQSVSGIVERRHTLPILSNVLLEKKGNQLT
                     LLATDIEIQITTTAICEGGDGDGAVTVGARKLQDILRSLPDSTEITVNLEDKRLQVKG
                     GKSR"
     gene            complement(529..1920)
                     /gene="dnaA"
                     /locus_tag="PLN02_07835"
     CDS             complement(529..1920)
                     /gene="dnaA"
                     /locus_tag="PLN02_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOI52271.1"
                     /translation="MTGFWESCLQRFEQELPAQQFNTWIKPLRLEGESTALEDGLRLI
                     APNGFILKWVRDRYLSRIEDYGATFFSAPVNISLVIGSGKPAASRAPSASPSAGGAPA
                     VPAVAVPEAAPPAARSKVASVYEKSRLFSNFTFDNLVVGRANDLARAAAVQVANNPGG
                     AYNPLFIYGGAGLGKTHLIHAIGNHIVRDNPEKIVRYVHAEDYYSDVVRAYQQKSFDT
                     FKRTYRSLDVLLLDDVQFFNGKNRSQEEFFFLFNALIEARKQIIITCDTYPKDINGLD
                     DRLVTRFDWGLTVQIEPPELEMRVAILKKKAEAEGIQLDDEVPFFIAKHLRSNVRELE
                     GALKKVLAYSSFHGRAIALDLAKEALKDVIGSVRNVGIDYIQKTVSDYYKIKVAEFFS
                     KKRTRAIARPRQVAMWLCREVTSHSFPEIGDAFGGRDHTTVIHAVKTIDSLRAKDNEL
                     NHDLHVLLQVLKG"
     gene            2156..2290
                     /gene="rpmH"
                     /locus_tag="PLN02_07840"
     CDS             2156..2290
                     /gene="rpmH"
                     /locus_tag="PLN02_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOI52272.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRSRTKGGRAVLAARRAKGRKRLAV
                     "
     gene            2309..2680
                     /gene="rnpA"
                     /locus_tag="PLN02_07845"
     CDS             2309..2680
                     /gene="rnpA"
                     /locus_tag="PLN02_07845"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOI52273.1"
                     /translation="MAASFARRYRLTKTDEYSSVFGFRKAIRGNWLMLHYLPRPEGDI
                     DARLGLVIGKKLLKRAVDRNQVKRIVREQFRLRRSALPVCDLIVRLATKPLPLDGKAI
                     AADINALFDRLCRPRAKRESA"
     gene            2677..2886
                     /gene="yidD"
                     /locus_tag="PLN02_07850"
     CDS             2677..2886
                     /gene="yidD"
                     /locus_tag="PLN02_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOI52274.1"
                     /translation="MKLLLIALVRFYQYAISPLFGRRCRYFPTCSEYTVDAIRKYGAI
                     KGGWLGIKRIFRCHPWHPGGYDPVP"
BASE COUNT          583 a          840 c          851 g          638 t
ORIGIN      
        1 agcggctctt gccgcccttg acctgcaggc gcttgtcttc gagattgacg gtgatctcgg
       61 tgctgtccgg cagtgagcgc aggatgtcct gcagcttgcg ggcgccgacg gtgaccgcgc
      121 catcgccgtc gccgccttcg caaatggcgg tggtggtgat ctggatttcg atatccgtag
      181 ccagcagggt cagctgatta cccttctttt ccagcagaac gttggaaagg atgggcagcg
      241 tgtgacgacg ttcgacaata cccgagaccg actgcaacgg agccagaagc gtgtctcttt
      301 ggctttttat aagaaccata aattaaattc ctttgtttac gatatcagga acagttctgt
      361 ggataactta aaaacatcgt ttttattcag caacctgcag aaaaaattca ggtcttggaa
      421 agcctctgga ttggctgtgg atgaaatccg gataattttc ccacaaagcg caaaagtcgg
      481 cttcatcccc agttcgccta cagtttaccc acagggttgt cgacagcctc atcccttgag
      541 tacctgcagc agtacgtgga ggtcgtggtt cagttcgttg tccttggcgc gcaggctgtc
      601 gatcgtcttc accgcgtgaa tgaccgtggt gtgatcgcgc ccgccgaaag catcgccgat
      661 ttccgggaag ctgtggctgg tgacttcgcg gcatagccac atcgccacct ggcgcggccg
      721 ggcaatcgcc cgcgtacgct tcttggagaa gaattcggcg accttgatct tgtaatagtc
      781 ggagacggtc ttctggatgt agtcgatgcc gacgttgcgc accgaaccga tgacgtcctt
      841 gagcgcttcc ttggccaggt cgagggcgat ggcacgaccg tggaaggagg aataggcgag
      901 caccttcttc agtgcccctt ccaactcgcg cacattggag cgcaaatgct tggcgatgaa
      961 gaagggaacc tcgtcgtcga gctggatgcc ttccgcctcc gccttcttct tcaggatggc
     1021 gacgcgcatt tccagttccg gcggctcgat ctgcaccgtc agcccccagt cgaagcgggt
     1081 caccagccgg tcgtcgagac cgttgatgtc cttcggatag gtatcgcagg tgatgatgat
     1141 ctgcttcctg gcttcgatca gcgcgttgaa caggaagaag aactcctcct gcgaacggtt
     1201 cttgccgttg aagaactgca cgtcgtcgag cagcagcaca tcgagcgaac ggtaggtgcg
     1261 cttgaaggtg tcgaaggact tctgctggta ggcgcggacg acgtcggagt aataatcttc
     1321 ggcatgcacg tagcggacga tcttctccgg attgtcgcgg acgatgtggt tgccgatggc
     1381 gtgaatcagg tgggtcttgc cgagaccggc gccgccgtag atgaacaacg ggttgtaggc
     1441 gccacccgga ttgttggcga cctgcacggc ggccgctcgg gccagatcgt tggcgcggcc
     1501 gaccaccagg ttgtcgaagg tgaaattgga gaacagccgc gatttctcgt aaaccgaagc
     1561 caccttgctg cgtgctgcgg gtggcgctgc ttcgggcacg gcaactgccg gtaccgctgg
     1621 cgcgccgccg gcagaagggc tggcgctcgg ggcgcggctg gccgccggtt tgccactgcc
     1681 gatcaccagc gagatgttga ccggcgccga aaagaaggtg gcgccgtagt cctcgatgcg
     1741 tgacagatag cggtcacgta cccatttcag gatgaagccg ttcggcgcga tcaggcgcaa
     1801 tccgtcttcc agtgccgtgc tctcaccttc caggcgcaat ggcttgatcc aggtattgaa
     1861 ctgctgcgct ggcagttcct gttcgaagcg ctgcaaacag gattcccaga aaccagtcat
     1921 tgaaggaaaa actccatgcc ccactgccac cgtcatggtg atcagtttta ttaattctat
     1981 gattggaaat cggatttcca atgaaatcgg cggctggtcg gccgccgaac gagggctaat
     2041 tctactcccc cggccacagg ttatccacag cgtcagcaaa taattcaagt tgacagcagt
     2101 ggtccaaaca aggtgtaatc acgcgttttt ctccgctcac tcaaggaatt acgacatgaa
     2161 acgcacgtat caaccttcgg tcgtgcgccg caagcgcacc catggcttcc tggtccgttc
     2221 gcgcaccaag ggcggccgtg cagtcctcgc tgcccgccgt gccaagggcc gcaagcgtct
     2281 ggccgtttaa taccggacta tcggcgaggt ggctgcaagc ttcgccagac gctatcgcct
     2341 gaccaaaacg gatgagtatt catccgtttt tggtttccgg aaggccattc gcggcaactg
     2401 gctgatgctg cattacctgc cgcggccgga aggcgatatc gatgcccggc tcggtctggt
     2461 catcggcaag aaactgctca agcgggcggt cgatcgcaac caggtgaaac gcatcgtgcg
     2521 cgaacagttt cgtctgcgtc gatccgccct gccggtctgc gacctgatcg tcaggctggc
     2581 gaccaagcca ttaccacttg acggcaaggc catcgctgcc gacatcaatg cgcttttcga
     2641 ccgtctgtgc cggccgcgcg caaaaaggga aagtgcatga agctgctcct catcgcactg
     2701 gttcgtttct atcagtacgc catcagtccg cttttcggac gacgttgccg ctattttccc
     2761 acctgttccg aatatacggt cgatgcgatc cgcaagtacg gcgccatcaa gggcggctgg
     2821 ctcggcatca aacgtatttt ccgttgtcac ccctggcatc cgggcgggta tgatcccgta
     2881 ccctgaaccc ccaacaaatc acgtaggcag cc
//
LOCUS       DAOSDX010000318         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12046_length_2911_cov_3.793067, whole genome shotgun sequence.
ACCESSION   DAOSDX010000318 DAOSDX010000000
VERSION     DAOSDX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12046_length_2911_cov_3.793067"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLN02_07855"
     CDS             complement(<1..234)
                     /locus_tag="PLN02_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HOI52275.1"
                     /translation="MTENFTLLFLAAFTLTLAARLWLKLRQIRHVAAHRGTVPTEFAE
                     RISLASHQKAAAYTIDRSRTAILGTLSEAVLLLA"
     gene            291..845
                     /gene="orn"
                     /locus_tag="PLN02_07860"
     CDS             291..845
                     /gene="orn"
                     /locus_tag="PLN02_07860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOI52276.1"
                     /translation="MAGNANNLVWLDMEMTGLNPDGDRIIEMAMVVTNSELEMVAESP
                     SWAVHQSDEVLAGMDDWNQKTHGRSGLIERVRTSTLAEADVEAAALEFLRKYSGKGHS
                     PMCGNSIGQDRRFMARYMPTLEAFFHYRNLDVSTLKELCKRWQPEVYRGFKKKSKHTA
                     LADIYESIDELKYYREHFLIKSGS"
     gene            complement(862..2481)
                     /locus_tag="PLN02_07865"
     CDS             complement(862..2481)
                     /locus_tag="PLN02_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52277.1"
                     /translation="MPDFAMLIRRGIRLPPGGWVLAGMLAFYVLAGLFGRDPWKGEDA
                     IHIGTAWQMLSSGDWLAPALAGRPFHEPPLYYWTAALTGMLFDWLLPAHEAMRLASGI
                     WIALALVGLYYAGRELYGTDSAAASPMLLAGCAGLLIHAHDAQPMLIALAAYAGGLGA
                     LATIGRKPRLTGVFYGLALAACLLGAGIAPTLPLLAIAPLAWWLSPDRAKALQTLLIG
                     LLIGTLIALPWPLTLHLLQPARFTAWLATELAPLQTPFSFVSLGRFAAMLPLFAFPAL
                     PLALWTLWTRRSQWYFPAQLLPLAFLLLTLLLLAWAYRPREIPALLLLPPLALLATPG
                     ALSLRRGAANAFDWFAMSVFSLFALLIWVAWSAMALGFPARLAERVVVLRPGFVGEIH
                     WLGLCIGGVATLFWLWLIATAPRSPYRSLTHWTLGFTTLWLIANALLLPWFDYGKSYR
                     PVAEAVARALPDGSRCLAERGLSQGQLASFAYFVDIAPIAHDSPAGRQCHWLLLVGSG
                     SELAAPGDGWTQVWEGRRPGERREKFRLYRR"
     gene            complement(2542..2766)
                     /gene="rpmE"
                     /locus_tag="PLN02_07870"
     CDS             complement(2542..2766)
                     /gene="rpmE"
                     /locus_tag="PLN02_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOI52278.1"
                     /translation="MKADIHPNYHDVQVTCSCGNVFTTKSTMPKNDFHIEVCAACHPF
                     YTGKQKIVDTAGRVEKFNQKFGAMRGKAAA"
BASE COUNT          574 a          854 c         1029 g          454 t
ORIGIN      
        1 cgcgagcagc agtacggctt cgctcagcgt gccaaggatc gccgtacggc tgcggtcgat
       61 cgtgtaggcg gcggctttct ggtgcgaggc gaggctgatg cgctcggcaa actctgtcgg
      121 tacggtgccc cggtgcgcgg cgacgtggcg gatttggcgc agcttgagcc agaggcgggc
      181 ggccagcgtg agcgtgaaag cggcaaggaa tagcagggtg aagttctcgg tcacgggcga
      241 tgtcggggaa gctgtgagaa aatggcgttt ttaaagtttc gggcaattat atggcaggca
      301 atgcaaacaa cctcgtctgg ctggacatgg agatgaccgg tctcaatccg gacggcgacc
      361 gcatcatcga gatggcgatg gtggtgacca attccgaact cgagatggtc gccgagtcgc
      421 cgtcatgggc ggtgcaccag tccgacgaag tgctcgccgg catggacgat tggaaccaga
      481 aaacgcacgg acgttccggt ttgatcgagc gcgtgcggac ttcgacgctg gccgaggccg
      541 atgtcgaggc agcggccctg gagttcctgc gcaagtactc gggcaagggg cattcgccga
      601 tgtgcggcaa ttcgatcggc caggatcgcc gcttcatggc ccgctacatg ccgacgctgg
      661 aagccttctt ccactaccgc aacctcgacg tcagtactct gaaggagttg tgcaagcgct
      721 ggcagccgga ggtgtaccgc ggtttcaaga agaagagcaa gcacaccgcg ctggccgata
      781 tctatgagtc gatcgacgaa ctcaagtact accgggagca tttcctgatc aagtccggta
      841 gctgagtttc tctgccggcg ctcagcgccg gtagagacgg aatttctcgc gccgctcgcc
      901 ggggcggcga ccttcccaga cctgcgtcca gccgtcaccc ggcgctgcca gttcgctgcc
      961 gctgccgacc agcagcagcc agtggcactg gcgcccggcc ggactgtcgt gcgcaatcgg
     1021 cgcaatatcg acaaaatagg cgaaggaggc cagttgcccc tggctcaggc cacgttcggc
     1081 caggcaacgg ctgccgtcgg ggagtgcccg ggccactgct tcggcgaccg gtcggtaact
     1141 cttgccgtaa tcgaaccagg gcagcagcag tgcgttggcg atcagccaca gcgtggtgaa
     1201 accgagggtc cagtgcgtca ggctgcggta cggcgagcgt ggcgcggtgg cgatcaacca
     1261 cagccagaac agggtggcga cgccgccgat gcacaagccg agccagtgga tttcaccgac
     1321 gaagccgggg cgcaggacga ccacgcgttc cgccaggcgg gccggaaaac cgagcgccat
     1381 cgccgaccag gcgacccaga tcagcagcgc gaagaggctg aaaacgctca ttgcgaacca
     1441 gtcgaaggcg ttggccgcac cgcgccgcag gctcaaggcg cccggtgtgg caagcagggc
     1501 gagcggcggc agcaacagca gggccgggat ctcgcgcggc cggtaggccc aggcgagcag
     1561 gagcagggtc agcagcagga aggccagtgg cagtagctgg gccgggaaat accactggct
     1621 gcgccgcgtc cacagggtcc agagcgccag cggcagggcc gggaaggcga acagcggcaa
     1681 cattgccgcg aaacggccaa gcgaaacgaa ggagaacggc gtttgcagcg gcgccagttc
     1741 ggtcgccagc caggcggtaa agcgcgccgg ttgcagcagg tggagcgtca gcggccaggg
     1801 cagggcaatg agggtgccga tcaatagccc gatcagcaaa gtctgcaaag ccttggcacg
     1861 gtccggcgac agccaccagg caagcggagc gatcgccagc aggggcaggg tcggcgcgat
     1921 accggcacca agcaggcagg cggccagcgc cagaccataa aaaacgccgg tcaggcgcgg
     1981 tttgcgaccg atcgtggcca gcgcgccgag accgccggcg taagcagcga gcgcgatcag
     2041 catcggctgg gcgtcgtggg cgtggatcag caggccggcg caaccggcca gcagcatcgg
     2101 actggccgcc gcgctgtcgg tgccgtacaa ctcgcgcccg gcgtagtaga gaccgaccag
     2161 cgccagcgca atccagatgc cgctggccag gcgcatggcc tcgtgcgccg gcagcagcca
     2221 gtcgaagagc atgccggtca gcgccgccgt ccagtaatac agcggcggct catggaaagg
     2281 ccggccggcc agcgctggcg ccagccagtc gccggacgac agcatttgcc aggcggtgcc
     2341 gatatggatc gcgtcctcgc ccttccacgg atcgcgaccg aagaggccgg cgaggacgta
     2401 gaaggcgagc atgccggcga gcacccagcc acccggaggc aggcggatgc cgcggcggat
     2461 cagcatggca aaatcaggca tcggtggcag aaatgaaaaa ggcagcccac gggctgccct
     2521 ttacggagtg ccgcaagccg atcaggcagc agccttgccg cgcatggcgc cgaatttctg
     2581 gttgaacttc tcgacgcgac cggcggtgtc gacgatcttc tgcttgccgg tgtagaacgg
     2641 gtggcaggca gcgcagactt cgatatggaa gtcgttcttg ggcatcgtgg acttggtggt
     2701 gaagacgttg ccgcaggagc aggtcacttg cacgtcgtgg taattcgggt gaatgtcggc
     2761 tttcatttca tatcctttag tcaagcgatc ggcggtattg gccgatctgg gaagcgcgtg
     2821 attatcgttg attcgggggg aaggagcaat tgttttttgg gttttgttgg ggggcgattg
     2881 tgtttttctg tgccgctgct tttcgccgtt g
//
LOCUS       DAOSDX010000319         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12057_length_2909_cov_2.818150, whole genome shotgun sequence.
ACCESSION   DAOSDX010000319 DAOSDX010000000
VERSION     DAOSDX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12057_length_2909_cov_2.818150"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLN02_07875"
     CDS             <1..1128
                     /locus_tag="PLN02_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOI52279.1"
                     /translation="RQEMEMVETVYLTEGRIPDLTGGSALYVPTLKAQPLPAAFAGLH
                     PGFQEVVGENGAWHVLETSIEGHRFILARDQGTFEAREQVLYKVVIGGFLLAVALAWL
                     IGRLTVRRVIDPVIRLAGQVRGRDQMVALAPPLAADYGDDELGQLAMAFDLAFSQLRL
                     AIDRERLFTSDVSHELRTPLMIVGTSAELLLRGDLSERARRQVERIARAAAEMRSLIE
                     VFLELARAQGAQLAPGEGVSLAAMADEQSQLWAEDIAARGLAFVRRSEGELPGLWHPT
                     LLRVVMGNLLRNAAHYTERGEIRLILASDGFRVEDTGPGISPAERAQLFQPFVRGRAA
                     RGEGLGLGLSLVRRICEQQGWRIDALNLDGGGSCFVVRLGG"
     gene            1214..1897
                     /locus_tag="PLN02_07880"
     CDS             1214..1897
                     /locus_tag="PLN02_07880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI52280.1"
                     /translation="MNPSSPIELEFSNKYDSAHALQYFHKHRAGLARRLSDWRDRQLA
                     RHALHLAGEPGLVLDLPCGAGRFWSLLGERPNRVILAADNSPHMLDVARRLQAPEIAG
                     RVHTFRTSAFAIDLDDGAVDCIFCMRLLHHIAESEHRLAMLREFHRVARDTVIVSLWV
                     DGNVKAWRRRQLEAKRAARGNANRNRFVVPVATIESEFAAAGFSILGHQDFLPGYAMW
                     RVYTLRKLA"
     gene            1986..>2909
                     /locus_tag="PLN02_07885"
     CDS             1986..>2909
                     /locus_tag="PLN02_07885"
                     /inference="COORDINATES: protein motif:HMM:NF023321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HOI52281.1"
                     /translation="MSALEVAGVLRRYFALLYVALFCMVALFWPQVTIRGGAAWGFTL
                     AASAAYAGLYLAAAALPCLLLSLLPRRFAFRQPLLAGAAVVGGSLMLLAMYADYRLYA
                     LYQYHFNGFVWNLLTTPGGVAALGATASTELTVAIQVSAIVLANLAVMWGLHRYRQLG
                     WQFPARPAVIAGFVLCGLLATEEVVFAYSVHTGQEELLAAADAIPFHMRTSSKTLFQK
                     LGVERRALQSLRLAGGDVVYPGTAIDSSHLKPTNVIVLVAESFRWDLLDPEITPNLWK
                     FSQRATRYDNHYSGGNRTRMGLFSLFYGVYAP"
BASE COUNT          407 a         1005 c          954 g          543 t
ORIGIN      
        1 cgccaagaga tggaaatggt cgagacggtc tacctgaccg aggggcggat tcccgacctc
       61 accggcggca gcgcgctcta cgtgccgacg ttgaaggcgc agcccctgcc ggcggcgttt
      121 gccgggttgc atccgggctt ccaggaagtg gtcggcgaaa acggcgcctg gcatgtgctg
      181 gaaaccagta tcgaagggca tcgcttcatc cttgcccgcg accaggggac tttcgaagcg
      241 cgcgagcagg tgctgtacaa ggtggtgatc ggcggcttcc tgctggcggt cgcgctggcc
      301 tggctgatcg gccggctgac cgtgcgccgg gtgatcgacc cggtgatccg gctcgccggg
      361 caggtgcgcg ggcgcgacca gatggtggca ttggcgccgc cgctggccgc cgattacggc
      421 gacgacgaac tcggccagct ggcgatggcc ttcgatctcg ccttcagtca gttgcgcctg
      481 gcgatcgacc gcgagcgtct gttcaccagc gacgtcagtc acgaactgcg cacgccgttg
      541 atgatcgtcg gcacttcggc cgaactgctg ctgcgcggcg acttgtccga acgcgcccgc
      601 cgtcaggtcg aacggattgc ccgggcggcg gcggaaatgc gctcgctgat cgaagtcttt
      661 ctcgaactgg cgcgcgccca gggcgcgcaa ctggcgcccg gcgaaggcgt cagcctggcg
      721 gcgatggccg acgagcaaag ccagctctgg gcagaggaca tcgccgcccg cggtctcgcc
      781 ttcgtccgcc gcagcgaggg cgagctgccc ggactgtggc acccgacgct gctgcgcgtg
      841 gtgatgggca acctgctgcg caacgccgcg cattacaccg agcgcggtga aatccgcctg
      901 atcctcgcca gcgacggttt ccgcgtcgag gataccgggc cggggatttc gcccgcggag
      961 cgggcgcagc tgttccagcc cttcgtccgc ggccgtgcag cgcgcggcga aggcctcggg
     1021 ctcggcctgt ccttggtgcg ccggatttgc gagcagcagg gttggcggat cgacgcgctc
     1081 aacctcgatg gcggcggcag ttgtttcgtg gttcgcctgg gcggctgatt tgacgttttt
     1141 ttgacgggct tttgacggcg gcttgacggg ccgccgccta tcgttggcgc catcatcaaa
     1201 ccggtgcgcg accatgaatc cgtcttcccc gatcgaactc gagttttcca acaaatacga
     1261 cagcgcgcat gcgctgcagt atttccacaa gcaccgcgcc ggcctcgccc gccgcctgtc
     1321 cgactggcgc gaccgccagc tcgcccggca cgcccttcat ctcgccggcg aacccggcct
     1381 ggtgctcgac ctgccctgcg gcgccggccg cttctggtca ttgctcggcg aacgtccgaa
     1441 ccgcgtcatc ctcgccgccg acaactcgcc gcacatgctc gacgtggcgc gccgcctgca
     1501 agcgccggaa atcgccggcc gcgttcacac cttccgcact tcggcctttg ccatcgacct
     1561 cgacgacggc gctgtcgatt gcatcttctg catgcgcctg ctgcatcaca tcgccgaatc
     1621 ggagcaccgg ctggcgatgc tgcgcgaatt ccatcgtgtc gcccgcgata cggtgatcgt
     1681 ctcgctctgg gtagacggca acgtcaaggc ctggcgtcgc cgccagctcg aggcgaagcg
     1741 cgcggcgcgc ggcaacgcca accgcaaccg cttcgtggtg ccggtggcaa cgatcgagag
     1801 cgagttcgcc gccgccggct tctccatcct cggtcatcag gacttcctgc ccggctacgc
     1861 gatgtggcgg gtgtacacgc tgcgcaagct ggcctgagcc ggtttccagc caagttcatg
     1921 gcctgttcct ccggcgccgg gggcaaggcc gcgtgtctgt ttcctggcgc cgttgcgagg
     1981 tatcgatgtc cgctcttgaa gtcgccggcg tgctgcgccg ttattttgca ctgctctatg
     2041 tcgcgttgtt ctgcatggtg gcgctgttct ggccgcaagt gacgatccgc ggcggtgccg
     2101 cctggggttt cacgctggcc gccagcgccg cctacgctgg tctctacctg gcggccgccg
     2161 ccttgccctg cctgctgctg tcgctgctgc cccgccgctt cgccttccgc cagccgctgc
     2221 tggccggtgc cgcggtcgtc ggcggcagcc tgatgctgct ggcgatgtac gccgattacc
     2281 gcctctatgc gctctaccag tatcacttca acggtttcgt ctggaacctg ctgacgacgc
     2341 caggcggcgt cgccgcgctc ggcgcgaccg cctccaccga gctgacggtg gcgatccagg
     2401 tgagcgccat cgtcctcgcc aacctggcgg tgatgtgggg gctgcatcgt taccgccagc
     2461 tcggctggca gttcccggcg cgtccggcgg tgatcgccgg cttcgtcctc tgtggtctgc
     2521 tggccacaga ggaagtggtg ttcgcctaca gcgtccatac cggtcaggaa gaactgctgg
     2581 cggcggccga cgcgattccc ttccacatgc ggaccagcag caagacgctg ttccagaagc
     2641 tcggcgtcga acgtcgcgcc ctgcagtcgc tgcgtctggc cggcggcgac gtggtctatc
     2701 cgggcactgc catcgacagc agccacctga agccgaccaa tgtcatcgtc ctggttgccg
     2761 aatccttccg ctgggatctg ctcgacccgg aaatcacccc caacttgtgg aaattctcgc
     2821 aacgggcgac ccgctacgac aaccactaca gcggcggcaa ccgtacccgg atggggcttt
     2881 tctcgctgtt ctacggggtt tatgcgccc
//
LOCUS       DAOSDX010000320         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12098_length_2902_cov_3.063576, whole genome shotgun sequence.
ACCESSION   DAOSDX010000320 DAOSDX010000000
VERSION     DAOSDX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12098_length_2902_cov_3.063576"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLN02_07890"
     CDS             <1..532
                     /locus_tag="PLN02_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI52282.1"
                     /translation="RPYLGVNVSVSDINAEDFPERVAAILAEEGMPAERLILEVTEDM
                     LLGDSGNVAQVLGRLRALGLRIAIDDFGTGYSSMSYLSSFMVNIIKIDQSFIRNLIDS
                     SSDQKIVRAIVSMAADLELSVVTEGVETPEQKELLRQMGCRLLQGYLFGRPQPAHSWL
                     GEKGLDLEAFQPFPAT"
     gene            complement(542..2164)
                     /locus_tag="PLN02_07895"
     CDS             complement(542..2164)
                     /locus_tag="PLN02_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HOI52283.1"
                     /translation="MLVAANITMQFGAKPLFENVNVKFGEGYRYGLIGANGAGKSTFM
                     KILCGALEPSAGNVSKDVNERMAYLRQDQFAYEEMRVLDVVMMGHEELWSAIQERDAI
                     YANPEATEDDYMRAAELEGKVGEYDGYTAESRAGELLLGVGIPTSQHNGPMSEVAPGW
                     KLRVLLCQALFANPDILLLDEPTNNLDINTIRWLEDVLNNRDSTMIIISHDRHFLNQV
                     CTHMADLDYGKITTYAGNYDDFMEAAQQARERQSSANAKAKERIAELQTFVRRFSANA
                     SKAKQATSRLKLIDKLKPEDVKPSSRQYPWIRFDYDEKQKLHRQAVEIENLTFAYEGS
                     ARKIVDNLTLTINGGEKIAVIGENGVGKTTFLKLLMGELQPQYGTLKWAEKAKLGYYA
                     QDHSAEFAGGQSLTDWIVGYARASIDEGGDLETLVRGTLGRLLFSGDEQKKAVNVISG
                     GEQGRMLFGRLMLQKPNVLVLDEPTNHLDMESIEALNSGLEKFNGTLVFVSHDREFVS
                     SLATRVLEVKGDGRIVDYLGGYEDYLASLGLE"
     gene            2308..>2902
                     /locus_tag="PLN02_07900"
     CDS             2308..>2902
                     /locus_tag="PLN02_07900"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOI52284.1"
                     /translation="MVPHLTTALSGPLLELEHRFLEASPQIEQWLRAQWQESTPPFYS
                     SVDLRNSGFKLAPVDTNLFPGGFNNLNPAFLPLCVQAAMSAIEKFCPEAKRLLLIPEN
                     HTRNQFYLQNVAQIAAVLKQTGLEVRIGSLIPDLAGPTPVDLPNGQQLLLEPLRRDGN
                     RIGVAGFEPCAILLNNDLSAGIPEILSDLDEQYVLPPL"
BASE COUNT          488 a          936 c          910 g          568 t
ORIGIN      
        1 ccgtccctac ctaggggtta acgtctcggt cagcgacatc aatgccgagg actttcccga
       61 acgcgttgcc gccatcctgg ccgaggaagg catgccggcc gaacggctga tcctggaagt
      121 caccgaagac atgctactcg gcgacagcgg caacgtcgcc caggtgctgg gacgcctgcg
      181 ggcgctcgga ctgcgtatcg ccatcgacga tttcggcacc ggctattcgt cgatgagcta
      241 cctctccagc ttcatggtga acatcatcaa gatcgaccag tccttcatcc gcaacctgat
      301 cgacagctcg tcggaccaga agatcgttcg cgcgatcgtg tcgatggcag ccgacctgga
      361 actgagcgtc gtcaccgaag gcgttgaaac ccccgagcag aaagaactgc tacgccagat
      421 gggctgccgc ctgctgcagg gttacctgtt cggccgaccg caaccggcac atagctggct
      481 gggcgaaaag ggattggacc tggaagcttt ccagcccttc cccgccacct agccggccag
      541 cttattcaag gccgagcgaa gccaggtaat cctcgtagcc gccgaggtaa tcgacgatgc
      601 ggccgtcgcc cttgacttcc agcacgcggg tggcgagcga ggagacgaat tcacggtcgt
      661 gcgagacgaa gaccagggtg ccgttgaact tttccaggcc gctgttgagc gcctcgatcg
      721 actccatgtc gaggtggttg gtcggttcgt cgagcaccag cacgttcggc ttctgcagca
      781 tcaagcggcc gaacagcatg cggccctgtt caccgccgga gatgacgttg acggctttct
      841 tctgttcgtc gccggagaag agcaggcggc cgagggtgcc gcggaccagg gtttccaggt
      901 cgccgccttc gtcgatcgag gcgcgggcat agccgacgat ccagtcggtc aggctctggc
      961 cgccggcgaa ttcggcgctg tggtcctgcg cgtagtagcc gagtttggcc ttttccgccc
     1021 acttcagcgt gccgtactgc ggctgcagtt cgcccatcag cagcttgagg aaggttgtct
     1081 tgccgacgcc gttctcgccg atcacggcaa tcttctcgcc gccgttgatg gtcagcgtca
     1141 ggttgtcgac gatcttgcgc gcgctgcctt cataggcaaa cgtcaggttc tcgatctcga
     1201 ccgcctggcg gtgcagcttc tgcttctcgt cgtagtcgaa gcggatccac gggtactggc
     1261 gcgacgacgg cttgacgtct tccggcttca gcttgtcgat cagtttcaga cggctggtcg
     1321 cctgcttggc cttggaggcg ttggccgaga aacggcggac gaaggtctgc agttcggcga
     1381 tccgttcctt ggccttggca ttggcgctcg actggcgctc gcgcgcctgc tgcgcggctt
     1441 ccatgaagtc gtcgtagttg ccggcatagg tggtgatctt gccgtaatcg aggtcggcca
     1501 tgtgggtgca cacctggttc aggaagtggc gatcgtgcga gatgatgatc atcgtcgaat
     1561 cgcggttgtt gagcacgtcc tccagccagc ggatggtgtt gatgtcgagg ttgttggtcg
     1621 gctcgtcgag cagcaggatg tccgggttgg cgaacagcgc ctggcagagc agcacgcgca
     1681 gcttccagcc gggggcgacc tcgctcatcg ggccgttgtg ctggctggtc gggatgccga
     1741 cgccgagcag cagttcgccg gcgcgcgact cggcggtgta gccgtcgtac tcgccgacct
     1801 tgccttcgag ctcggcggcg cgcatgtagt cgtcctcggt cgcctccggg ttggcgtaga
     1861 tcgcgtcgcg ttcctggatc gcgctccaca actcctcgtg gcccatcatg accacgtcga
     1921 gcacgcgcat ctcttcgtag gcgaactggt cctgacgcag gtaggccatg cgctcgttga
     1981 cgtccttgga gacgttgccg gccgacggtt cgagcgcccc gcacaggatc ttcatgaagg
     2041 tcgacttgcc ggcgccgttg gcgccgatca ggccgtagcg gtagccttcg ccgaacttga
     2101 cgttgacgtt ttcaaagagg ggcttggccc cgaattgcat ggtgatgttg gcagcgacga
     2161 gcacgacggt tccgatccgg caagtatttc aataaaggcg ggattttacc ctttttgcgc
     2221 ggctggccgc ggccgccggc cccattttgt gcaccgcggt gtaacatcgc ggccgacaat
     2281 aatgcgccag aacccaggag acagcccatg gtcccccatc tcaccacggc cctgagcggc
     2341 ccgctgctag aactggaaca ccgcttcctc gaagccagtc cgcagatcga acagtggctg
     2401 cgcgcccagt ggcaggaatc gacgccgccc ttctattcgt cggtcgacct gcgcaactcc
     2461 ggcttcaagc tggcgccggt cgataccaac ctgttcccgg gcggcttcaa caacctcaac
     2521 ccggccttcc tgccgctctg cgtgcaggcg gcgatgagcg cgatcgagaa gttctgtccg
     2581 gaagccaagc gcctgctgct gatcccggaa aaccacacgc gcaaccagtt ctacctgcag
     2641 aacgtcgccc agatcgccgc cgtcctcaag cagaccgggc tggaagtgcg gatcggctcg
     2701 ctgatccccg acctcgccgg accgaccccg gtcgacctgc cgaacggtca gcaactgctg
     2761 ctcgaaccgc tgcgccgcga cggcaaccgg atcggcgtcg ccggcttcga gccctgcgcg
     2821 atcctgctca acaacgacct ctcggccggc attcccgaga tcctcagcga cctcgacgag
     2881 cagtacgtgc tgccgccgct gc
//
LOCUS       DAOSDX010000321         2899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12114_length_2899_cov_3.161744, whole genome shotgun sequence.
ACCESSION   DAOSDX010000321 DAOSDX010000000
VERSION     DAOSDX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12114_length_2899_cov_3.161744"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /gene="atpA"
                     /locus_tag="PLN02_07905"
     CDS             complement(<1..1216)
                     /gene="atpA"
                     /locus_tag="PLN02_07905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOI52285.1"
                     /translation="MQLNPSEISELIKSKIQNLQGASEVRTQGTVVSVTDGICRVHGL
                     QDVMQGEMLEFPGNTIGMALNLERDSVGAVILGEYEHISEGDTVKTTGRILEVPVGPE
                     LLGRVVNTLGQPIDGKGPINAKETDKLEKVAPGVIWRKSVSQPVQTGLKCVDSMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGKNLFCVYVAVGQKASTIANVVRKLEEHGAM
                     EYTIVVAASASESAALQYLAPYAGCSMGEYFRDRGQDALIIYDDLTKQAWGYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVSEAWVERFTNGAVTGKSGSLTALPVIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLSGG
                     IRTDLAQYRELAA"
     gene            complement(1226..1759)
                     /locus_tag="PLN02_07910"
     CDS             complement(1226..1759)
                     /locus_tag="PLN02_07910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOI52286.1"
                     /translation="MAESVTIARPYADAAFKLAKESGAQGIWSQRLQRLALIAQDGDM
                     AQVMGNPRLSAEQIAELFISLSEDSDPILANFVRTLAENRRLALLPEISRLFDLARSQ
                     EEGVQEAVVYSAFPIDDAQVAALLQQLEPRFGTRLTARVEVDASLIGGVRVTVGDQML
                     DASVRGKLDAMAVALNN"
     gene            complement(1765..2235)
                     /locus_tag="PLN02_07915"
     CDS             complement(1765..2235)
                     /locus_tag="PLN02_07915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOI52287.1"
                     /translation="MNLNATLFAQLVVFFILAWFTMQFVWPPIVKALDERAKKIADGL
                     AAAERGKQDLELATKRSTEALREGKEKAAELIANAEKRGAQLIEEAKAAAKVEAERIV
                     TGAKAEIEQEAVRAKEQIREQVAALVINGAEQILRREINAQAHSDILATIKQDL"
     gene            complement(2278..2532)
                     /gene="atpE"
                     /locus_tag="PLN02_07920"
     CDS             complement(2278..2532)
                     /gene="atpE"
                     /locus_tag="PLN02_07920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOI52288.1"
                     /translation="MEHVLGFVALAAGLIIGLGAIGACIGIGLMGGKYIEASARQPEL
                     MNALQTKMFLLAGLIDAAFLIGVGIAMMFAFANPFQLVAG"
     gene            complement(2611..>2899)
                     /locus_tag="PLN02_07925"
     CDS             complement(2611..>2899)
                     /locus_tag="PLN02_07925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOI52289.1"
                     /translation="LWPINFAMQLIEFVAKTVSHGMRLFGNMYAGELIFMLIALMGAA
                     WAGTFGSGLLWLGGVIAGTIWAIFHILIITLQAFIFMMLTLVYIGQAHEAH"
BASE COUNT          593 a          842 c          896 g          568 t
ORIGIN      
        1 acgcagccag ttcacggtac tgggccaggt cggtacgaat accgcccgag agcttcttga
       61 tgaccttggt ctgcgcagca ccacccacac gcgacaccga gataccggcg ttgatcgcgg
      121 gacggatacc agcgttgaag aggtcggttt ccaggaagat ctgaccgtcg gtgatggaga
      181 tcacgttggt cggaacgaaa gcggaaacgt caccggcttg cgtttcgatg accggcagcg
      241 cggtcagcga accgctcttg cccgtcacgg cgccgttggt gaaacgctcg acccaggctt
      301 cggaaacgcg ggcagcgcgt tccagcaggc gggagtggag gtagaagacg tcgcccgggt
      361 aggcttcacg gcccggcgga cggcgcagca gcagggaaac ctgacggtaa ccccaggcct
      421 gcttggtcag atcgtcgtaa atgatcaggg catcttgacc acggtcacgg aagtattcgc
      481 ccatcgagca accggcgtag ggagccaggt actgcagcgc ggccgattcc gaagcggagg
      541 cggcgacgac gatggtgtat tccatggcgc cgtgctcttc gagcttgcgc acgacgttgg
      601 cgatggtgga agccttctgg ccgacggcga cataaacgca gaacaggttc ttgcccttct
      661 ggttgatgat cgcatcgaca gcgacggcgg tcttgccggt ctggcggtcg ccgatgatca
      721 gttcgcgctg gccgcggcca accggaacca tcgagtcgac gcacttcaga ccggtctgca
      781 ccggctgcga aaccgactta cgccagatga cgcccggggc gaccttttcc agcttgtcgg
      841 tttccttggc gttgatcggg cccttgccgt cgatcggctg gccgagggtg ttgaccacgc
      901 ggccgagcag ttccggacca accggcactt ccaggatgcg gccggtcgtc ttgaccgtat
      961 cgccttcgga gatgtgctcg tattcgccca ggataacggc gccgacggag tcgcgttcga
     1021 ggttgagcgc catgccgatg gtgttgccgg ggaattccag catttcgcct tgcatgacgt
     1081 cctgcaagcc atgcacgcgg cagataccgt cagtgacgga aaccacggtg ccctgcgtac
     1141 gcacttccga tgcgccttgc aggttctgga tcttgctctt gatcagttca gaaatttcgg
     1201 aaggattcaa ctgcatgaaa atctcctagt tgttcagtgc cacggccatc gcgtcaagct
     1261 tgccgcggac tgaagcatcc agcatctggt caccgacggt aacccgcaca ccaccgatca
     1321 ggctcgcatc gacttcgacg cgggcggtca ggcgggttcc gaaacgcggc tccagttgct
     1381 gcagcagagc agcgacctgg gcgtcgtcaa tcgggaatgc ggaatacacc accgcctcct
     1441 ggaccccttc ttcctggctg cgcgccagat cgaaaagccg ggaaatctcc ggcaggagtg
     1501 cgagacgccg gttttctgcg agggtacgaa caaaattggc gagaatcggg tcgctgtctt
     1561 cggacagcga aatgaacaat tctgcaatct gttcagcgga gagccgggga ttgcccatga
     1621 cctgagccat gtccccgtcc tgggcgatca gcgccagcct ttgcaggcgc tgcgaccaga
     1681 tgccctgggc cccgctttcc ttggccagct tgaaagcggc gtcggcgtag gggcgagcga
     1741 tggtgacgga ttcagccata agcgttacag atcctgtttg atcgtggcca gaatgtcgga
     1801 atgagcctgg gcgttgatct cgcggcgcag aatttgttcg gcgccgttga tgaccagggc
     1861 agcgacctgt tcgcgaatct gctctttggc gcgcaccgct tcctgctcga tttctgcctt
     1921 ggcaccggtc acgatgcgct cggcttccac cttggccgca gccttggctt cttcgatcag
     1981 ttgggcgccg cgcttttcag cattggcgat cagttcagca gccttttcct tgccttcgcg
     2041 gagagcttcc gtcgaacgct tggtggccaa ttcgagatcc tgcttgccgc gttcagcagc
     2101 agccagacca tccgcgatct tcttcgcacg ctcgtcgagc gccttcacaa tggggggcca
     2161 cacgaattgc atcgtgaacc acgccagaat gaagaacaca acgagctgtg caaacaacgt
     2221 tgcgttcaga ttcacggtgt tagtcctctt ctaaagggtt gatcggaaga ggagtaatta
     2281 acctgcgacc agttggaacg ggttggcaaa ggcgaacatc atggcgatac cgacgccgat
     2341 caggaaggcg gcatcgatca gaccggccag caggaacatc ttggtctgca gggcgttcat
     2401 cagttccggc tgacgagccg aagcttcaat gtacttgccg cccatcaggc cgataccgat
     2461 acaggcaccg atagcaccca gaccaatgat caggccggca gccagagcaa caaaaccgag
     2521 aacgtgttcc atgacaactc cttgctaagt ttaagtaatg aaaaggtaaa gcgaaaacag
     2581 ggttactacg ttgaatctgt tgggtgagtc ttagtgtgct tcgtgcgcct gaccgatgta
     2641 gaccagggtc agcatcatga agatgaaggc ctgcagggtg atgatcagga tgtggaagat
     2701 ggcccagatg gtgccggcga tcacgccgcc gagccagagc aggccgctgc cgaaggtgcc
     2761 ggcccaggcg gcgcccatca gcgcgatcag catgaagatc agttcgccgg cgtacatgtt
     2821 gccgaacaga cgcatgccgt gcgaaacggt cttggcgacg aactcgatca gctgcatggc
     2881 gaagttgatc ggccacaga
//
LOCUS       DAOSDX010000322         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12145_length_2893_cov_3.960183, whole genome shotgun sequence.
ACCESSION   DAOSDX010000322 DAOSDX010000000
VERSION     DAOSDX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12145_length_2893_cov_3.960183"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2893)
                     /gene="dnaE"
                     /locus_tag="PLN02_07930"
     CDS             complement(<1..>2893)
                     /gene="dnaE"
                     /locus_tag="PLN02_07930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOI52290.1"
                     /translation="RNRQGYQQLCELLTRAYTTEGRRDRAEIRREWFAEIGCDGLIAL
                     SGAHLGDVGEALLNGNFDLATERAQAWEAIFPDAFYLEVQRYGQPQQEAIVQQTADLA
                     GETGIPLVATHPIQFMNRDDFRAHEARVCIAEGYVLGDHRRPKIYTEEQYFKSQDEMV
                     ELFADLPEALENTLEIARRCNIEMTLGKNFLPNFPIPPGMTIDEFLVDEAKKGLEVRL
                     AELYPDPEVRQQRRPEYDARLAFECNTIVQMGFPGYFLIVADFINWAKQNGVPVGPGR
                     GSGAGSLVAYSLRITDLDPLAYALLFERFLNPERVSMPDFDVDFCQDNRWRVIEYVRE
                     HYGADAVSQIATFGTMSSKAVIRDVGRVFGLPYSMCDRISKLIPIVQNKPVSLAEALE
                     QEPQLREMMEGDGDGETIRELFDLAGRLEDLTRNVGMHAGGVLIAPGKITEFCPIYQA
                     TGADASPVSQFDKDDVEKAGLVKFDFLGLRNLTIIELALKYIERMTGEKLDLMSLGFE
                     DPGAYQILKDANTTAIFQVESEGMKKLLKKLAPDRFEDIIAVLALYRPGPLGSGMVDD
                     FILRKKGQQAIDYFHPDLTACLAPTYGVIVYQEQVMQISQIIGGYTLGGADMLRRAMG
                     KKKPEEMAKHRETIAAGAKEKGYDPALAEQLFDLMTKFAEYGFNKSHTAAYAVVTYHT
                     AWLKKYHCAAFMAATMSSDMDNTDTVKIFYEDTVANKVKVLGPDVNASAYRFEPVDRA
                     TIRYGLGAVKGTGEQAVNDILRAREAGGPFKDLFDFCQRCDKRMVNRRTIEALIKAGA
                     FDSIDADRHKLLASVGIAMEFAEQAERNAMQTSLFDLGDVAEAHAPQYLTVKPWDEKT
                     QLMEEKTALGFFFSGHPYNSYKKELGRFVRRPLNRLEPSKEFQVLAGVVVGVRTQMTR
                     RGKMLFVQLDDGTTMVEVSVFNELFEAERGKIVTDEILLVEGKVRYDDFS"
BASE COUNT          459 a          881 c          985 g          568 t
ORIGIN      
        1 ggagaagtcg tcgtaacgga ccttgccttc gacgagcagg atttcgtcgg tgacgatctt
       61 gccgcgttcg gcctcgaaca actcgttgaa caccgagact tcgaccatcg tcgtcccgtc
      121 gtcgagctgg acgaagagca tcttgccgcg gcgcgtcatc tgcgtgcgca cgccgaccac
      181 gacgccggcc agcacctgga actccttgga cggttcgagc cggttcaacg ggcgccggac
      241 gaagcgcccc agttccttct tgtagctgtt gtacgggtga cccgagaaga agaagccgag
      301 cgccgtcttc tcctccatca actgcgtctt ctcgtcccag ggcttgaccg tcaggtactg
      361 cggcgcgtgc gcctcggcga cgtcgccgag gtcgaacagg ctggtctgca tcgcgttgcg
      421 ctcggcctgc tcggcgaact ccatggcgat gccgaccgag gcgagcagct tgtggcggtc
      481 ggcgtcgatg ctgtcgaagg caccggcctt gatcagcgcc tcgatggtgc ggcggttgac
      541 catgcgcttg tcgcaacgct ggcagaaatc gaacaggtcc ttgaacgggc cgccggcctc
      601 gcgcgcgcgc aggatgtcat tgaccgcctg ctcgccggtg cccttgaccg caccgaggcc
      661 gtagcggatg gtcgcgcggt ccaccggttc gaaacggtaa gccgaagcat tgacgtcggg
      721 accgaggacc ttgaccttgt tggcgacggt gtcctcgtag aagatcttca ccgtgtcggt
      781 gttgtccatg tcggacgaca tggtcgccgc catgaaggcc gcgcagtggt atttcttcag
      841 ccaggcggtg tgataagtga cgacggcgta ggcggcggtg tgcgacttgt tgaagccgta
      901 ttccgcgaac ttggtcatca ggtcgaacaa ctgctcggcg agcgccggat cgtagccctt
      961 ttccttcgcg ccggcggcaa tcgtctcgcg gtgcttggcc atttcctcgg gctttttctt
     1021 gcccatcgcg cggcgcagca tgtcggcgcc accgagcgtg tagccgccga tgatctgcga
     1081 gatctgcatg acctgttcct ggtacacgat gacgccgtag gtcggcgcca ggcaggcggt
     1141 caggtcgggg tggaaatagt cgatcgcctg ctggcctttt ttgcgcagga tgaagtcgtc
     1201 gaccatcccg gagccgagcg ggccgggacg atagagcgcg agcacggcga tgatgtcttc
     1261 gaaacggtcg ggtgccagct ttttcagcag cttcttcatg ccttccgatt cgacctggaa
     1321 gatcgccgtc gtgttggcat ccttcaggat ctggtaggcc cccgggtctt cgaagccgag
     1381 cgacatcagg tcgagcttct cgccggtcat gcgctcgatg tacttgagcg ccagctcgat
     1441 gatcgtcagg ttgcgcaggc cgaggaagtc gaacttgacc aggccggcct tttcgacgtc
     1501 gtccttgtcg aactgcgaga ccggtgaggc gtcggcgccg gtggcctggt agatcgggca
     1561 aaactcggtg atcttgcccg gcgcgatcag cacgccgccg gcgtgcatgc cgacgttgcg
     1621 ggtcaggtct tcgaggcggc cggccaggtc gaacagttcg cggatggttt cgccgtcgcc
     1681 gtcgccctcc atcatctcgc gtagctgcgg ctcctgctcc agcgcctcgg ccagcgacac
     1741 cggcttgttc tggacgatgg ggatcagctt cgagatgcgg tcgcacatcg aataaggcag
     1801 gccgaagacg cggccgacgt cgcggatcac cgccttcgac gacatggtgc cgaaggtggc
     1861 gatctggctc accgcgtcgg cgccgtagtg ctcgcgcacg tactcgatga cgcgccagcg
     1921 gttgtcctgg cagaagtcga cgtcgaagtc gggcatcgag acccgttccg ggttgaggaa
     1981 gcgttcgaac agcagcgcgt aggcgagcgg gtcgaggtcg gtgatgcgca ggctgtaggc
     2041 aaccagcgag ccggcgccgg agccccgccc gggaccgacc ggcacgccgt tctgcttggc
     2101 ccagttgatg aagtcggcaa cgatcaggaa gtagccgggg aagcccatct ggacgatggt
     2161 gttgcactcg aaggcgagcc gcgcgtcgta ttccgggcgg cgttgctggc ggacttccgg
     2221 gtcaggatag agttcggcaa gacggacttc gaggcccttc ttcgcctcgt cgacgaggaa
     2281 ttcgtcgatc gtcatgccgg gcgggatcgg gaagttgggc aggaagttct tgcccagcgt
     2341 catctcgatg ttgcagcggc gggcgatttc cagcgtgttt tccagcgctt ccggcaggtc
     2401 ggcgaacagc tcgaccattt cgtcctgcga cttgaagtac tgctcctcgg tgtagatctt
     2461 cggccgccgg tggtcgccca gcacgtaacc ttcggcgatg cagacgcgcg cctcgtgggc
     2521 gcggaagtcg tcgcggttca tgaactggat cgggtgcgtg gcgaccagcg ggatgccggt
     2581 ttcgccggcc aggtcggcgg tctgctggac gatcgcttcc tgctgcggct ggccgtagcg
     2641 ctgcacttcg aggtagaagg catcgggaaa aattgcctcc caggcctgcg cacgttcggt
     2701 cgccaggtcg aaattgccgt tcagcagcgc ttcgccgaca tcgccgagat gcgcgccgga
     2761 gagggcgatc aggccgtcgc aaccgatttc ggcgaaccat tcgcggcgga tctcggcgcg
     2821 gtcgcgccgg ccctcggtgg tgtaggcgcg ggtcagcagt tcgcacaact gctggtagcc
     2881 ctggcggttg cgc
//
LOCUS       DAOSDX010000323         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12186_length_2885_cov_2.796466, whole genome shotgun sequence.
ACCESSION   DAOSDX010000323 DAOSDX010000000
VERSION     DAOSDX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12186_length_2885_cov_2.796466"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..458)
                     /locus_tag="PLN02_07935"
     CDS             complement(42..458)
                     /locus_tag="PLN02_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOI52291.1"
                     /translation="MANITRVDPFDDLFRGFFVRPVDFNGQPQQAPSIKMDVREQGDA
                     YLVHAELPGVKKEDIHVVVDGNQVSISAEVRQEKEVKEGERTLRSERYFGKVSRSFQL
                     QQDLDDTHSVARFADGVLELTLPKRIASPSKRLTIE"
     gene            complement(534..983)
                     /gene="nudB"
                     /locus_tag="PLN02_07940"
     CDS             complement(534..983)
                     /gene="nudB"
                     /locus_tag="PLN02_07940"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HOI52292.1"
                     /translation="MSAYKQPHSVLVVIHTLAGEVLLLERARHPGFWQSVTGSREGNE
                     PPAVTAHREVLEETGIDSRLYRLTDWRIENHYTIHPAWRHRYAPGVDCNTERVFGLCL
                     PERVAVRIAPEEHRAWLWLARDEAAERCFSWSNRDAIRLLGVAHEWT"
     gene            complement(980..1363)
                     /locus_tag="PLN02_07945"
     CDS             complement(980..1363)
                     /locus_tag="PLN02_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOI52293.1"
                     /translation="MNEFAPSGVYRQHADHPIDADITVDLGACREARQVLDRLGTTLD
                     FPDWYGSNFDALADCLSDPDWRDGAATLIRLSGLTRFSQQSPGDYAILLAVLDAACQA
                     RSEQGAPLAVILEDGPETLPPWLAA"
     gene            complement(1367..1723)
                     /locus_tag="PLN02_07950"
     CDS             complement(1367..1723)
                     /locus_tag="PLN02_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HOI52294.1"
                     /translation="MRQLMRFLIVLLLAIGSAFAFWSGGGDRQTIDVARLPPEARETL
                     VLIKQGGPFPYKRDGIVFNNFEKLLPLQPRGYYREYTVKTPGRRDRGPRRIVAGKAAE
                     YYYTEDHYRSFRRIRE"
     gene            complement(1727..>2885)
                     /gene="aspS"
                     /locus_tag="PLN02_07955"
     CDS             complement(1727..>2885)
                     /gene="aspS"
                     /locus_tag="PLN02_07955"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOI52295.1"
                     /translation="KCFRDEDLRADRQPEFTQVDIETSFMSEAEITALMEQLIRRVFK
                     EAIDVELPEFPHMTYAEAMRRFGSDKPDLRVTLELVDVADAFKDVAFKVFANIANSEG
                     GRIAAMRIPGGASLTRGEIDEYTKFVGIYGAKGLAYIKVNDVTQVNETGLQSPIVKNL
                     SEASLRTVLERTGAQSGDLIFFGADKAKIVSDALGALRIKIGHEKGFVTGAKWAPLWV
                     IDFPMFEYDDESKRWTACHHPFTSPKDEHLEFLATDPGKCLAKAYDLALNGWELGGGS
                     VRIHRADVQEKVFAALNIGPEEQQAKFGFLLDALKYGAPPHGGLAFGLDRIVTMMTGA
                     ESIRDVIAFPKTQRAQCLLTDAPSAVDEKQLRELHVRLRQKVETQVEVAQG"
BASE COUNT          459 a          833 c         1030 g          563 t
ORIGIN      
        1 cggccggctt gggggccggc cggcgcgagg ggtaaggcgg cttactcgat ggtcaggcgc
       61 ttgctcgggc tggcgatgcg tttgggcagg gtcagttcaa gtacgccatc ggcgaagcgg
      121 gcgaccgagt gggtgtcgtc gagatcctgc tgcagttgga aggagcgcga gaccttgccg
      181 aaatagcgct ctgatcgaag tgtgcgctcg ccttccttga cttccttttc ctgacggact
      241 tcggcgctga tggaaacctg gttgccgtcg acgacgacat ggatgtcttc cttcttcacg
      301 ccgggcagtt cggcgtgcac gaggtaggcg tcgccttgtt ccctgacgtc catcttgatc
      361 gagggggctt gctggggctg cccgttgaaa tcgacggggc ggacgaagaa gccgcggaac
      421 agatcgtcaa aggggtcgac acgggtgatg ttggccatct tggttctcct ttccggtggg
      481 ttttgaaata aggggattca tgcaaaacca atataggtct gccgccccgg ttttcaagtc
      541 cactcgtgcg cgacgccgag caagcggatc gcgtcccggt tgctccacga gaagcagcgc
      601 tcggcggcct cgtcgcgcgc tagccagagc caggcgcggt gctcctcggg cgcgatgcgg
      661 acggcgacgc gctcgggcag gcagaggccg aaaacccgct cggtgttgca gtcgacgccg
      721 ggcgcgtagc ggtggcgcca ggctggatgg atcgtgtagt ggttttcgat gcgccagtcg
      781 gtgaggcggt aaagccggct gtcgatgccg gtttcctcga gcacctcgcg gtgggccgtg
      841 actgccggcg gttcgttgcc ttcgcggctg ccggtcactg actgccagaa gccgggatgg
      901 cgggcgcgct cgagcagcag gacttcaccg gccagggtat ggatgaccac cagtaccgaa
      961 tgcggctgct tgtaggcgct catgccgcca gccatggggg cagggtttcc ggtccgtctt
     1021 cgaggatgac ggcaagcggc gcgccctgtt cgctgcgcgc ctggcaggcg gcgtcgagta
     1081 cggcgagcag tatcgcgtag tcgcccggcg actgctgcga gaaacgggtc agcccgctca
     1141 gccggatgag ggtggcagcg ccgtcgcgcc agtcggggtc ggagaggcaa tcagccaggg
     1201 catcgaaatt acttccgtac cagtcgggaa aatccagggt ggtgccgagt cgatcgagga
     1261 cttgcctggc ctcacggcag gcgccgaggt cgaccgtgat gtctgcatca atggggtggt
     1321 cggcatgctg gcgatagacg ccactggggg cgaattcatt cattggctat tcccggatgc
     1381 gccggaagga gcggtagtgg tcttccgtgt agtaatactc ggcggccttg ccggcaacga
     1441 tgcggcgtgg gccgcgatcc ctgcggccgg gtgtcttcac cgtgtactcg cggtaatagc
     1501 cgcgcggttg cagcggtagc agcttttcga aattgttgaa gacgatgccg tcgcgcttgt
     1561 aggggaaagg gccgccctgc ttgatcagga ccagcgtttc gcgggcttcg ggcggcaggc
     1621 gggcgacatc gatcgtctgc cggtcgccgc cgccactcca gaaagcaaaa gcgcttccga
     1681 tcgccagcag caggacgatc aggaagcgca tcagctggcg catggtttag ccctgggcaa
     1741 cttcgacctg ggtttcgacc ttctgccgca ggcggacgtg cagctcgcgc aactgcttct
     1801 cgtcgaccgc cgacggggcg tcggtgagca ggcactgggc gcgctgggtc ttcgggaagg
     1861 cgatcacgtc gcggatcgat tcggcgccgg tcatcatggt gacgatgcga tccaggccga
     1921 aggccaggcc gccgtgcggc ggggcgccgt acttgagcgc gtcgagcagg aagccgaact
     1981 tggcctgttg ttcttccgga ccgatgttga gcgcggcgaa gaccttttcc tgtacgtcgg
     2041 cgcggtggat acgcaccgag ccgccaccca gttcccagcc gttcagcgcc aggtcatagg
     2101 ccttggccag gcacttgccc ggatcggtcg ccaggaattc gaggtgctcg tccttcgggc
     2161 tggtgaaggg gtggtggcag gcggtccacc gcttgctctc gtcgtcgtat tcgaacatcg
     2221 ggaagtcgat gacccacagc ggcgcccact tggcgcctgt gacgaagccc ttttcgtggc
     2281 cgatcttgat gcgcagcgcg ccgagggcgt cgctgacgat cttggccttg tcggcgccga
     2341 agaagatcag gtcgccggac tgcgcgccgg tgcgttcgag cacggtgcgc agcgaggctt
     2401 cggagaggtt cttgacgatc ggcgactgca ggccggtttc attgacctgg gtgacgtcgt
     2461 tgaccttgat gtaagccagg cccttggcgc cgtagatgcc gacgaacttg gtgtattcgt
     2521 cgatctcgcc gcgggtcagc gaagcgccgc ccggaatgcg catggcagcg atgcggccgc
     2581 cttcgctgtt ggcgatgttg gcgaagacct tgaacgcaac gtccttgaag gcgtcggcga
     2641 catcgaccag ttcgagggtg acgcgcaggt ccggcttgtc cgagccgaaa cgacgcatgg
     2701 cttcggcgta ggtcatgtgc gggaattccg gcagctcgac gtcgatcgct tccttgaaga
     2761 cgcggcggat cagttgttcc atcagggcgg tgatttcggc ctcgctcatg aacgaggttt
     2821 cgatatcgac ctgggtgaat tcgggctggc ggtcggcgcg caggtcttcg tcgcggaagc
     2881 acttg
//
LOCUS       DAOSDX010000324         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12197_length_2883_cov_2.967115, whole genome shotgun sequence.
ACCESSION   DAOSDX010000324 DAOSDX010000000
VERSION     DAOSDX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12197_length_2883_cov_2.967115"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLN02_07960"
     CDS             complement(<1..460)
                     /locus_tag="PLN02_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52296.1"
                     /translation="MITVEDCNAFCDAPLGQVEEVARREGLPPVLAFARAQTQGGFAA
                     PRHAMMPFERKETMMMTMQARAMFPLPSEPRHPLRAYPINSASAKARLVVLALLADGR
                     LDECELEVLNRRGSFAALGISRAGFVQVLHEFCADVSRLAGGAADWRVSPD"
     gene            582..1493
                     /locus_tag="PLN02_07965"
     CDS             582..1493
                     /locus_tag="PLN02_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI52297.1"
                     /translation="MKPDDLNFRHLLYFWAVAKEGSITRAAERLGLSVQAISTQLGQL
                     ERQLGQALLAPRGRSLTLTEAGRTALAHADQIFQLGDRLRDALAEPSAGRIRLAVGIT
                     DAVPKLIAFRVLEGVLGAHPQLRLECSEGEFDHLLGELAVNRLDLVLADRSAPQRANL
                     RLQSKLLGNLAIALYAAPQLHGQYASSFPAGLQGAPLLLPVRGDPLRAGIDAWLEAHD
                     IVPQVIGEFSDSALLKTFGRAGLGIFPAPLALQEDIHLQFGALPIGELSGVSESWYAI
                     ATQRRLQHPAVDTLFEAGGGFIVGGAG"
     gene            1598..>2883
                     /locus_tag="PLN02_07970"
     CDS             1598..>2883
                     /locus_tag="PLN02_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOI52298.1"
                     /translation="MEKIGFWSGIAGAWPLCLLALTVNALAAGIDCAKASSLGERAIC
                     ADEQLLRLDGLLAEIHAHLQRALPDQAAGLRRAQKDWLLSRDQCAGNSSCLEQLYRER
                     IGVLSTQWQATVATTMPDRIDRQALQVLRAAVETRLQSDREFPLEKTLDALRIRQGMT
                     HFNNTLDAGEDDQVARFPRHRPPGVTPDEWQALRNSPIDGGGEHGQASYTLYDLDGDG
                     RRDLIVDSYVGGTGLFNYISVLRRQGERFTGAHAANAPLYSLNGRGSNQQAVWIRLAG
                     RVYAAYRNSVYGQDDLYLLRPFGLPGKVPTLTLHYRYDWSVPTEQRGDGRPPFTLDAP
                     THAALTRALAGFVRQDSAGAGAPDELLCPMPEGADPASRSRYASFGPGHYTYEMLGDV
                     PVWIGQECLLGRVVDWFGNYAADSGLFAALWTSHPET"
BASE COUNT          460 a          945 c          961 g          517 t
ORIGIN      
        1 ggtcgggcga gacacgccag tcggcggcac cgccggccag ccgcgagacg tcggcgcaga
       61 actcgtgcag gacctggacg aagcctgcgc ggctgatgcc cagcgcggcg aagctgccgc
      121 gccggttgag cacttccagc tcgcattcgt cgagccggcc gtcggcgagc agggcgagga
      181 ccaccaggcg cgccttcgcc gacgcactgt tgatcgggta ggcgcgcagc gggtggcgag
      241 gttcgctggg cagcgggaac atggcacggg cctgcatcgt catcatcatc gtttcctttc
      301 gttcgaacgg catcattgca tggcgtgggg cagcgaaacc tccctgcgtc tgggcgcggg
      361 caaaagccag caccgggggc agtccctcgc ggcgggcgac ttcctcgacc tggccgagcg
      421 gggcatcgca aaaggcgttg caatcttcga cggtgatcat gggggggctc ctctgggtat
      481 cggctgcggg aagtccgcgg cacatgggcg caatttacgg ccgcatcgat ttccaatgaa
      541 tggataatat tggtgttcca acttccgttt tatggaaacg tatgaagccc gatgatctca
      601 acttccgtca tctgctctac ttctgggcgg tcgccaagga aggcagcatc actcgcgccg
      661 ccgagcgtct cggcctgtcg gtgcaagcga tcagcaccca gctcggccag ctcgaacgcc
      721 agctcggtca ggccctgctg gcgccgcgcg gccgttcgct gaccctgacc gaagcggggc
      781 ggaccgcgct cgctcacgcc gaccagatct tccagctcgg cgaccgtctg cgcgatgcgc
      841 tggccgagcc gtcggccggc cgcatccggc tcgccgtcgg catcaccgac gcggtgccca
      901 agctgatcgc cttccgcgtg ctcgaaggcg tcctgggcgc gcatccgcaa ctgcgcctcg
      961 aatgcagcga aggcgaattc gaccacctgc tcggcgaact cgcggtcaat cggctcgacc
     1021 tcgtgctcgc cgaccgctcg gcgccgcaac gcgccaatct gcgcctgcag tcgaaactgc
     1081 tcggcaatct ggcgattgcc ctttacgcgg caccgcagct gcatggccag tacgccagca
     1141 gtttccccgc cggcctgcag ggcgcgccgc tgctgctgcc ggtgcgcggc gacccgctgc
     1201 gcgccggcat cgatgcctgg ctggaggccc acgacatcgt gccgcaggtg atcggcgagt
     1261 tttccgacag cgccctgctc aagaccttcg gtcgcgccgg cctcggcatc tttccggcgc
     1321 cgctcgccct gcaggaagac atccacctcc agttcggcgc gctgcccatc ggcgagctgt
     1381 cgggcgtcag cgaaagctgg tacgcgatcg ccacccagcg ccgcctgcag catccggccg
     1441 tggacacgct gttcgaggcg gggggcggat tcatcgttgg cggtgcgggc tgaatcgcca
     1501 gcgctcagcg gcagtgaatt tctcgggcaa ggaatggcgg tttgtcaggg tgttgaggtt
     1561 aaattgcgga tgtcatgaat tctgtggggc gttgcgcatg gagaaaatcg ggttctggtc
     1621 gggtatcgcc ggcgcttggc cgctctgcct gttggcactg acggtgaatg ccttggcggc
     1681 gggcatcgac tgcgccaagg cgagtagtct tggcgagcgc gcaatctgtg ccgacgagca
     1741 gttgttgcgt ctggatggct tgctcgccga aatccatgcc catctgcaac gcgccttgcc
     1801 ggatcaggct gccggcttgc ggcgggcgca aaaggactgg ctgctcagcc gcgaccaatg
     1861 cgccggcaac tcgagctgct tggagcagct ttacagagag agaattggcg ttctgtccac
     1921 gcaatggcag gctactgtcg ctacgacaat gccggatcgg attgaccgac aggcactgca
     1981 ggtcttgcgc gcggcggtcg aaacgcgtct gcagagcgat cgcgagttcc ccctggaaaa
     2041 gactctcgac gccttgcgca tccgccaggg aatgacgcac ttcaacaaca cgctcgacgc
     2101 cggggaggac gatcaggtcg cccgctttcc ccgccatcgg ccgcccgggg tgacgcccga
     2161 cgagtggcag gcgctgcgca attccccgat cgatggcggc ggcgagcatg gtcaggccag
     2221 ttacaccctg tacgacctcg acggcgacgg ccgacgcgac ctgatcgtcg attcctatgt
     2281 cggtggtact ggcctgttca actacatcag tgtcctgcgg cgccagggtg agcgtttcac
     2341 cggcgcccac gccgccaatg ctcccctgta ttcgctcaac ggtcgcggca gcaaccagca
     2401 ggccgtgtgg atccgactgg ctggccgggt ttatgctgcc taccggaaca gcgtctatgg
     2461 ccaggacgat ctttacctgc tgcgtccttt cggcctgcca ggcaaggtgc cgacgctgac
     2521 gttgcactat cgttatgact ggtcggtgcc gacggagcag cggggcgatg gccggccgcc
     2581 cttcacgctc gacgcgccaa cccatgcggc attgacgcgg gcgctggccg gcttcgtccg
     2641 gcaggactcc gctggcgccg gggcgccgga tgaacttctg tgcccgatgc cggagggggc
     2701 cgaccccgcg tcgcgcagca gatatgccag ctttggcccg ggacattaca cctatgagat
     2761 gctcggcgac gtgccggtgt ggatcggtca ggaatgcctg cttggtcgtg tcgtcgactg
     2821 gtttggcaat tacgctgccg acagcggcct gtttgccgca ctatggacga gtcaccccga
     2881 gac
//
LOCUS       DAOSDX010000325         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12249_length_2870_cov_2.823091, whole genome shotgun sequence.
ACCESSION   DAOSDX010000325 DAOSDX010000000
VERSION     DAOSDX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12249_length_2870_cov_2.823091"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..247)
                     /locus_tag="PLN02_07975"
     CDS             complement(47..247)
                     /locus_tag="PLN02_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52299.1"
                     /translation="MDPRLTEETLFWIRLAAFAHLSEQILCSGASVADAEELLFGWSL
                     PGALPATYACLTGGVAYATNPG"
     gene            complement(421..894)
                     /locus_tag="PLN02_07980"
     CDS             complement(421..894)
                     /locus_tag="PLN02_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52300.1"
                     /translation="MNRHLPPLLAAALITVSPGLAAHDDFAATFAAKTKADINFAQLM
                     QIMQRATSMILDGILAENKEMTVLGAHLLDAHPAPAQGPGAAMVPEQREAFKNVMPAY
                     DKVFHSATERLSVAASRGQWPQAYADYREIMDACMACHETWRPYALSRQQTAGKR"
     gene            complement(923..>2870)
                     /locus_tag="PLN02_07985"
     CDS             complement(923..>2870)
                     /locus_tag="PLN02_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52301.1"
                     /translation="MNAILGLTHLMRRDAVLPRQFEHLDKIGDAAQHLLGIINDILDF
                     SKIESGKLALENADFDLDGLFRSIHVLIGDKAADKNLEVVTRIDPALPMMLCGDRMRL
                     GQILLNFANNAVKFTEHGVVSLRARKVDAPRPEEIRIRFEISDTGIGITPEQQSRLFQ
                     PFEQADLSTTRNFGGTGLGLAISRRLAELMGGRVGVTSAPDQGSTFWAEIPFQPARSP
                     AAQIQAGRGIERRLRVLVVDDLDEARDALVDMLLMLQLQGASVSGGQQAIQQVAAAAQ
                     RGQPFDLVLLDWKMPGLDGSETARQIQALHLPRPPLMILVTAYGRDEPADQLHDAGIR
                     LSLSKPVTPSTLHDAILESLSGEATTQPLPVSALDLGRLRGRRVLLAEDNPINQEVAL
                     ELLREVGLAVDLAEDGLAAVELARKNTYDLILMDMQMPRLDGIAATIQIHRLPGASET
                     PILAMTANAFAEDRQACLNAGMADHVAKPVDPEQLYSTMLRWLPAQALTAPAASSAAD
                     PEVQDQIARLKQMLGGIEGLNVDAGLRAVHGKLATYRRVIELFIATHADDASRLRELI
                     LAGQLREGEQLAHALKGSAANLGAGEIRYLAERIEQALRRSEIHEAEVVLSMLEIKLP
                     MMVSALAGKRESTTEGDANLAREV"
BASE COUNT          457 a          830 c         1042 g          541 t
ORIGIN      
        1 ccgcgcgcgg cactcgatct gcagccgcat tccggcacta cctgaatcag cccggattgg
       61 tcgcgtaagc gacaccgccg gtcaggcagg cgtaggtcgc aggcagggct ccgggcagcg
      121 accagccgaa caacagttcc tcggcatcgg caacgctggc gccggagcag aggatctgtt
      181 cggacaggtg agcgaaagcg gccagccgga tccagaacag ggtttcttcg gtcagtcggg
      241 ggtccatgat catctcccgg gaagcagtgc gcacaatcat gcaacatctg ttccatcagg
      301 agtgatcgca gccactaacg gaacaccagg tcaaaaccgg cacaagccag gtgcgtggcc
      361 ggcgtttatc gccgcctggc ggtgcgtgga ccgggcctgt tcagcgccgg tggcgggtgg
      421 ctagcgcttg ccggcagtct gctggcggga aagcgcgtac gggcgccagg tttcgtgaca
      481 ggccatgcag gcgtccatga tttcccggta gtcggcgtag gcttgcggcc actgtccccg
      541 gctggcggcg accgacaggc gctcggtcgc gctgtggaag accttgtcgt aggcgggcat
      601 cacattcttg aaggcttccc gctgttccgg gaccatggcg gcgccggggc cctgggccgg
      661 tgccgggtga gcgtcaagga gatgggcgcc gagtaccgtc atttccttgt tctcggccag
      721 gatgccatcg aggatcatcg acgtggcgcg ctgcatgatc tgcattagct gggcaaaatt
      781 gatgtcggct ttggtcttgg cggcaaaggt ggctgcgaaa tcgtcatggg cggccagccc
      841 cgggctgacg gtgatcaggg cggcggcaag caggggaggg aggtggcgat tcatggtgtc
      901 tgtgtgatga aaaaataatt catcatacct ctcttgctag attagcgtct ccttctgtag
      961 tgctttcgcg tttgccggcg agcgccgaaa ccatcatcgg tagcttgatt tcgagcatgc
     1021 tgaggacgac ttccgcttcg tggatctcgc tgcgccgcag ggcttgttcg atgcgttcgg
     1081 cgagatagcg gatttcgccg gcgccgaggt tggcggcgga acccttgagg gcgtgggcga
     1141 gttgttcgcc ctcgcggagc tgaccggcga ggatcagttc gcgcaggcgg ctggcatcgt
     1201 cggcgtgggt ggcaatgaag agttcgatga cccggcggta ggtggccagc ttgccgtgta
     1261 cggcgcgcag cccggcgtcg acgttgagtc cttcgatgcc gccaagcatc tgcttgaggc
     1321 gggcaatctg atcctggact tcgggatcgg cggcgctgct tgcggcggga gcggtcagcg
     1381 cttgcgccgg cagccagcgc agcatggtgc tgtagagctg ctcgggatcg accggcttcg
     1441 ccacgtggtc ggccatgccg gcattgaggc aggcctggcg gtcttcggcg aaggcattgg
     1501 cggtcattgc caggatcggc gtttcgctgg cgccgggcag gcgatggatc tggatggtgg
     1561 cggcgatgcc gtcgagacgc ggcatctgca tgtccatcag gatcaggtcg taggtgtttt
     1621 tcctggccag ttcgacagca gccaggccat cctcggcgag gtcgacggcg aggccgacct
     1681 cacgcagcaa ttcgagtgcg acctcctggt tgatcgggtt gtcttccgcc agcagcacgc
     1741 ggcgtccccg caaacggcca agatcgagtg cgctgaccgg cagcggctgg gtggtcgctt
     1801 cgccgctcaa ggattcgagg atcgcgtcgt gcaaggttga aggggtgacc ggcttgctca
     1861 ggctgaggcg gatgccggca tcgtgcagtt gatcggccgg ttcgtcgcgg ccgtaagcgg
     1921 tgaccaggat catcaacggc gggcgcggca gatgcagcgc ctggatctgt cgtgccgtct
     1981 cgctgccgtc gaggccgggc attttccagt caagcaggac gaggtcgaag ggctggccgc
     2041 gttgtgcggc ggcggcgact tgctggatcg cctgctggcc gccgctgacg ctggcgccct
     2101 gcaactgcag catgagcagc atgtcgacca gcgcgtcgcg cgcttcgtcg aggtcgtcga
     2161 caacgaggac gcgcaggcgg cgctcgatgc cgcggcccgc ttggatttgc gcagccggac
     2221 tccgggcggg ctggaaggga atctccgccc agaaggtgct gccctggtcc ggcgcgctgg
     2281 tgacgccgac gcggccgccc atcaattcgg cgagacgccg ggaaatggcc agaccgagac
     2341 cggtgccgcc gaagttgcgg gtggtcgaca ggtcggcctg ctcgaagggt tggaacaggc
     2401 gcgactgctg ctccggggtg atgccgatgc cggtatcgct gatttcgaaa cggatgcgga
     2461 tttcctccgg ccgcggagca tcgaccttgc gggcgcgcag gctgacgacg ccgtgctcgg
     2521 tgaacttgac cgcgttgttg gcgaagttga gcaggatctg gccgagccgc atgcggtcgc
     2581 cgcagagcat catcggcagt gccggatcga tgcgggtgac gacttccaga ttcttgtccg
     2641 ccgccttgtc gccgatcagc acgtggatgc tgcggaacag gccgtcgagg tcgaagtcgg
     2701 cgttttccag ggcgagcttg ccggattcga tcttcgagaa atcgaggatg tcgttgatga
     2761 tgcccaagag gtgctgggcg gcgtcgccga tcttgtcgag gtgttcgaac tgccgcggca
     2821 gcaccgcgtc acgccgcatc aggtgggtga ggccgagaat ggcgttcatc
//
LOCUS       DAOSDX010000326         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12261_length_2869_cov_2.417200, whole genome shotgun sequence.
ACCESSION   DAOSDX010000326 DAOSDX010000000
VERSION     DAOSDX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_2869_cov_2.417200"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLN02_07990"
     CDS             complement(<1..304)
                     /locus_tag="PLN02_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOI52302.1"
                     /translation="MSLSRRILAFCLVLCASLARAEPLTVGVSLLPMQTFVERIGGEH
                     VRAVVLVPPGKSPASYEPTPKQMQALAAAPLYFRIGVPFEKAWLPRLQQALPGLRLI"
     gene            complement(603..1811)
                     /locus_tag="PLN02_07995"
     CDS             complement(603..1811)
                     /locus_tag="PLN02_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HOI52303.1"
                     /translation="MTDQRQRLKVLGAGIFSLLLALGVARFAYTPLLPLMQQQAGLGV
                     ADAGWLAAINYGGYLSGALLASLISDLVLKDRLFRLGMLLAVASTALMGLTTEPWLWA
                     VSRFVAGLSSAAAMLFGTGLILNWLIRHKHRSELGIHFAGIGLGIAGCAAAVALMQQW
                     LDWRQQWFALTALGVLLAVPALRWLPPPDTSGVTRSGEKLEDNPPSPLFLRLFMAAYF
                     CAGVGYVVSATFIVAIVDRLPGLAGSGTLVFLALGLAAAPACIVWDFVARRTGELNAL
                     ILAALLQIVGILLPLAGGLLPALAGAMLFGGTFIGMVSLVLTMAGRYYPTRPAKMMGK
                     MTLSYGLAQILGPAITGWLAAGRAGDYAVGLYLAAAVMLAGCALLFLLKAVQRRDAGG
                     AVAPCACPQN"
     gene            1912..2760
                     /locus_tag="PLN02_08000"
     CDS             1912..2760
                     /locus_tag="PLN02_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI52304.1"
                     /translation="MELVALRTFQAVVEEGGTLAAARKLNTVQSNVTARIRRLEEELG
                     AELFFRKGRGLELAPPGRVLLDYSRRLLMLERQTAAAVRQVGEATGELRIGAMETFAA
                     LHLPRALKAVRAAHPGIELRVFTDTSARLADRVIQHKLDCACIAGPVIHPELEFSELL
                     VEELVQVHAAGHDPLQQPLILFREGCAYRTRALAWQRSQGHALADAMEFGTLEGILGC
                     VAVGLGWTLMPRRVVEQSPHAADLVVETLPPAIAAVPTGMIRLREAPPLAALATLAAA
                     LAEGAA"
BASE COUNT          484 a          913 c         1042 g          430 t
ORIGIN      
        1 cgatcaggcg cagtccgggc agcgcctgct gcaggcgggg cagccaggcc ttctcgaacg
       61 gcacgccgat gcggaaataa agcggcgcgg cagccagtgc ctgcatctgt ttcggcgtcg
      121 gttcgtagct ggccgggctc ttgccgggcg gcaccaggac gacggcccgg acatgctcgc
      181 cgccgatgcg ttcgacgaag gtctgcatcg gcagcaggct gacaccgacg gtcagcggct
      241 cggcgcgggc gaggctggcg cagaggacga ggcagaaggc caggatgcga cgggagagag
      301 acatgggaag gacctcgacg ggaattgcgc gaattcgtga tgttataaca taacattttc
      361 cgataaagcg ccgaccgcag ctttcgcgtt tttcttgcct ggtctccccc gaaaaggcga
      421 aagccccgta ccggaggggg agtacggggc tttctggacg gtccgggggg tcgggggaga
      481 gggaggtgac cgtcagggct gatgtctgtt cagcacatgg gttcagaata agtaggcggc
      541 cggccggcgg caagtaacgg tttgcaacgg cgcgtatcgg tccgtggtcg ccgtcgcgcc
      601 gttcagttct gcgggcaggc gcaaggcgca acggcgcccc cggcgtcgcg ccgctgcact
      661 gccttgagca ggaagagcag ggcgcagccg gcgagcatca ccgccgccgc caggtagagg
      721 ccgaccgcgt agtcgccggc gcggcccgcc gccagccagc cggtgatcgc cgggccgagg
      781 atctgcgcca ggccgtagga aagcgtcatc ttgcccatca tcttcgccgg tcgcgtcggg
      841 tagtagcggc cggccatggt caggaccagg ctgaccatgc cgatgaaggt gccgccgaac
      901 agcattgccc cggccagcgc cggcagcagg ccgccggcca atggcagcag gatgccgacg
      961 atctgcagca gcgcggcgag gatcagcgcg ttcagttcgc ccgtgcggcg ggcgacgaaa
     1021 tcccagacga tgcaggccgg tgccgccgcc aggccgagcg ccaggaagac cagcgtgccg
     1081 ctgccggcga ggccgggcag gcggtcgacg atggcgacga tgaaggtggc gctgaccacg
     1141 tagccgacgc cggcgcagaa ataggcggcc atgaacagac gcaggaagag cggcgacggt
     1201 gggttgtcct caagcttttc gccgctgcgc gtgacgccgc tggtatcggg cggcggcagc
     1261 cagcgcagcg ccggcacggc gagcaggacg ccgagcgcgg tcagcgcgaa ccactgctgg
     1321 cgccagtcca gccactgttg catcagtgcc acggcggcgg cgcaaccggc gatgccgagc
     1381 ccgatgccgg cgaagtggat gcccaattcg ctgcggtgct tatggcggat cagccagttg
     1441 aggatgaggc cggtgccgaa cagcatcgcc gccgcgctgc tcaggccggc gacgaagcgc
     1501 gacaccgccc acagccaggg ttcggtagtc agccccatca gtgccgtgct ggccacggcg
     1561 agcagcatgc cgaggcggaa caggcggtcc ttcagcacca ggtcgctgat cagcgacgcg
     1621 agcagggcgc cgctcaggta gccgccgtag ttgatcgccg ccagccagcc ggcgtcggcg
     1681 acgccgagtc cggcctgttg ctgcatcagc ggcagcagcg gcgtgtaggc gaaacgggcc
     1741 acgccaagcg ccagcagcag gctgaagatg ccggcgccca ggactttcag tcgttgtcgt
     1801 tggtcggtca ttgttgttgt ctcctcggtt ggtgaaacaa cgataaacgg gctaagctat
     1861 cgctacaatt gatttgttta gatacaaccg ttcgtttttg gagattcatt catggagctg
     1921 gtcgccctca ggactttcca ggcggtggtc gaggaaggtg ggaccctcgc cgcggcgcgc
     1981 aagctgaaca ccgtgcagtc caacgtcacc gcgcgcatcc gccgcctgga agaggaactc
     2041 ggtgccgagc tcttcttccg caaggggcgg ggcctggaac tggcgccgcc cggtcgcgtc
     2101 ctgctcgact acagccgccg cctgctgatg ctggagcggc agacggcggc ggcggtgcgc
     2161 caggtcggcg aggcgactgg cgaactgcgc atcggcgcca tggaaacctt tgccgcgctg
     2221 cacctgccgc gtgccctgaa agcggtgcgt gccgcccatc ccggcatcga gctgcgggta
     2281 ttcaccgata ccagcgcccg cctcgccgac cgggtgatcc agcacaaact cgactgcgcc
     2341 tgcatcgccg ggccggtgat ccacccggaa ctggaattca gcgaactgct ggtcgaggaa
     2401 ctggtgcagg tccatgccgc tggccacgat ccgctgcagc agccgctgat cctgttccgc
     2461 gaaggttgcg cctaccgcac ccgggcgctt gcctggcagc gcagccaggg gcatgcgctg
     2521 gccgacgcga tggagttcgg cacccttgaa ggcattctcg gctgcgtcgc ggtcggcctc
     2581 ggctggacgc tgatgccgcg gcgcgtcgtc gagcagtcgc cgcacgcggc ggatctggtg
     2641 gttgaaacgc tgccgccagc aatcgccgcc gtcccgaccg gcatgatccg cctgcgcgag
     2701 gcgccgccgc tggcagcgtt ggcgacgctg gccgcggcgc tggcggaagg cgccgcctga
     2761 aaaggcgaaa gccccgtacc ggggggagta cgaggctttc tggacggtct gttggggtcg
     2821 ggggagaggg aggtgaccgt caggctgatg tctggtcagc gcatgggtt
//
LOCUS       DAOSDX010000327         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12303_length_2862_cov_3.429640, whole genome shotgun sequence.
ACCESSION   DAOSDX010000327 DAOSDX010000000
VERSION     DAOSDX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12303_length_2862_cov_3.429640"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLN02_08005"
     CDS             <1..345
                     /locus_tag="PLN02_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HOI52305.1"
                     /translation="GRLMELLAEEIRPGDASFADQAFMVGIMSLMPALVGLPIRDIVT
                     PLPIADSVKAALCDGHGILAKLLGITVCSESGDLTGLAEQLAQQPELGAKALNRAQTA
                     ALAWAGTLASEK"
     gene            complement(387..998)
                     /locus_tag="PLN02_08010"
     CDS             complement(387..998)
                     /locus_tag="PLN02_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HOI52306.1"
                     /translation="MFKPVLSSALLAAALAASLPAQAADQGSFMVRARAVHINFENDQ
                     SNAVKTLDIAAQNRWIPEVDLSYFFTPNIAAELVLTYPQKVKVDAGSTKIGTIKALPP
                     SLMLQYHFTNLGAFKPYVGLGVNYTVFYDRGNFVIPGADVERSSFGVAAQVGFDYALD
                     KNWSVNMDVKYIQMDTDVKVGNTKLGNLDLNPITAGIGIGYRF"
     gene            1239..1556
                     /locus_tag="PLN02_08015"
     CDS             1239..1556
                     /locus_tag="PLN02_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52307.1"
                     /translation="MPRASPPFSSDIRFARAPQGIAGKLLTALFSVGLLVLGVMFSLV
                     ALAVAAVIGLLFAGWFWWKTCALRKALREATPAAATASRAADGDIIDGECVRETPSRE
                     LLR"
     gene            complement(1561..1866)
                     /locus_tag="PLN02_08020"
     CDS             complement(1561..1866)
                     /locus_tag="PLN02_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52308.1"
                     /translation="MTRALLLIAALACGPALAAELDRTPLDAVTADGQPVRLFPNGRW
                     EYVDQAKAAAAQKVAADYPENKTRPVESQGIVFGGIGRYVMPGDKDYNRGSLNPKLR"
     gene            complement(1863..>2862)
                     /locus_tag="PLN02_08025"
     CDS             complement(1863..>2862)
                     /locus_tag="PLN02_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOI52309.1"
                     /translation="TVGNTVQAKVDLVSRAATVRNHSATHLMHKALREVLGAHVQQKG
                     SQVDPDKTRFDFAHNSPLSAEEIREVEEIVNAEILANAATDSRVMGIDDAQKSGAMML
                     FGEKYGDEVRVVAIGSSKELCGGTHVARSGDIGLFKIVSEAGVAAGVRRVEAITGNNA
                     LAYVQTQESRVAGISALLKTQPDDIGERVAGILDHVRALEKEVARLKSKLAASQGDDL
                     LAQAVEVKGARLLATTLDGADVATLRETMDKLKDKLKSAAIVLASVADGKVTLIAGVT
                     ADLTGKVKAGELVNLVAQQVGGKGGGRPDMAQAGGTQPENLPAALASVAAWVEGKL"
BASE COUNT          503 a          931 c          896 g          532 t
ORIGIN      
        1 ggccgcctga tggaattgct cgccgaagag atccggccgg gcgatgccag ttttgccgat
       61 caggccttca tggtcggcat catgtcgttg atgccggccc tggtcggcct gccgatccgc
      121 gacatcgtca ccccgctgcc gatcgccgac agcgtcaagg ccgcgctctg cgatggccac
      181 ggcatcctgg ccaaactcct gggcatcacc gtgtgcagcg aaagcggcga cctgaccggg
      241 ctggccgaac aactcgcgca acagcccgaa ctcggcgcca aggccctgaa ccgtgcgcaa
      301 accgctgcgc tggcatgggc cggcacattg gcgtcggaaa aatgaaaaag gggccgaagc
      361 cccctttgcc aaccggctgc tgacgcttag aagcggtagc cgatgccgat accggcggtg
      421 atcgggttga gatcgagatt gccgagcttg gtattgccaa ccttgacgtc ggtatccatc
      481 tggatgtact tgacgtccat gttcaccgac cagttcttgt ccagcgcgta atcgaagcca
      541 acctgggctg ccacaccgaa gctggaacgc tcgacatctg cccccggaat gacgaaattg
      601 ccgcggtcgt agaacaccgt gtaattcacg ccaaggccga catagggctt aaaggcgccg
      661 agattggtga agtgatactg cagcatcagg gaaggcggca gcgccttgat cgtgccgatc
      721 ttggtcgatc cggcatcaac cttgaccttc tgcggatagg tcaggaccag ttcggcagcg
      781 atgttcgggg tgaagaagta gctgaggtcg acttccggaa tccagcggtt ctgcgccgcg
      841 atatccaggg tcttgaccgc gttgctctgg tcgttttcga agttgatatg aaccgcacgg
      901 gcccgcacca tgaagctccc ctgatcggcg gcctgggccg gcagcgaagc cgccagtgcg
      961 gcggcaagca gggcggacga aagaactggc ttgaacatga acactccctt tgtcgttgta
     1021 gaaaacgacg atgctagacc tctgcacatt ccggatattg acccaaatca aaatgcaata
     1081 aacaatttat gcggtttttt ctgatagaaa aaacattatc acatcctaat attccgaatt
     1141 catgcaacgc gctgttacaa aacggcgcgt tcgaagcaca acgcaaccag ccacttgagg
     1201 gacaatagcc acttttcagg acgagccaga ccccagccat gcccagagcg tcacccccct
     1261 tttcatctga catccggttt gcgcgtgccc cgcaaggcat cgctggcaaa ctgctgaccg
     1321 cgctgttcag cgttggcctg ctggtgctcg gcgtgatgtt ctcgctggtg gcgctggcag
     1381 tggcggcggt gatcggcctg ctcttcgccg gctggttctg gtggaagacg tgcgccctgc
     1441 gcaaggcgct gcgcgaagcg acgccggcgg cagcgaccgc gagccgcgcc gccgacgggg
     1501 acatcatcga cggcgaatgc gtccgcgaaa ccccgtcccg cgaactgctg cgctgagcgc
     1561 tcagcgcagc ttcggattca gcgaaccgcg gttgtaatcc ttgtcgcccg gcatcacgta
     1621 gcggccgatg ccgccgaaca cgatgccctg cgattcgacc ggacgggtct tgttttccgg
     1681 gtagtcggcg gccactttct gcgctgccgc ggccttcgcc tggtccacat actcccagcg
     1741 accgttcgga aacagccgga ccggctggcc atcggcggtc accgcgtcga gcggcgtccg
     1801 gtcgagttcg gccgccagcg ctggcccgca ggccagcgcg gcgatcagca gcagcgcgcg
     1861 cgtcacagct tgccttcgac ccaggcggcg accgaggcca gcgccgccgg cagattttcc
     1921 ggctgggtgc cgccggcctg cgccatgtcc ggccgaccgc cacccttgcc gccgacctgc
     1981 tgggcgacca ggttgaccag ctcgccggcc ttgaccttgc cggtcaggtc ggcggtgacg
     2041 ccggcgatca gcgtcacctt gccgtcggcc accgacgcca gcacgatggc cgccgatttc
     2101 agcttgtcct tcaacttgtc catcgtctcg cgcagcgtcg ccacgtcggc gccgtcgagt
     2161 gtggtcgcca gcagcctggc gcccttgacc tcgaccgcct gcgccagcag gtcgtcgccc
     2221 tgcgaagcgg ccagtttgga tttcagacgg gcgacttcct tttccagcgc gcggacatgg
     2281 tcgaggatgc cggcaacgcg ctcgccgata tcgtccggct gcgtcttcaa cagcgccgaa
     2341 atcccggcga cgcggctttc ctgggtctgc acataggcca gcgcgttgtt gccggtgatc
     2401 gcttcgacac ggcgcacgcc ggcagcgacg ccggcttccg agacgatctt gaacagaccg
     2461 atgtcgccgc tgcgggcaac gtgggtaccg ccgcacagtt ccttggagga gccgatggcg
     2521 acgacacgca cttcatcgcc gtacttttcg ccgaagagca tcatcgcgcc cgacttctgg
     2581 gcgtcgtcga tgcccatcac gcggctgtcg gtggcggcgt tggcgagaat ctcggcgttg
     2641 acgatttcct cgacctcgcg gatttcctcg gcgctcagcg gcgaattgtg cgcgaagtcg
     2701 aaacgggtct tgtccggatc gacctgcgag cccttctgct ggacgtgggc gccgagcacc
     2761 tcgcgcagcg ccttgtgcat gaggtgggtg gcggaatggt tgcgcaccgt ggcggcgcgc
     2821 gagaccaggt ccaccttggc ctgcacggtg ttgccgacgg tg
//
LOCUS       DAOSDX010000328         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12318_length_2860_cov_3.543316, whole genome shotgun sequence.
ACCESSION   DAOSDX010000328 DAOSDX010000000
VERSION     DAOSDX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12318_length_2860_cov_3.543316"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLN02_08030"
     CDS             complement(<1..720)
                     /locus_tag="PLN02_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52310.1"
                     /translation="MQSSGNSGFQRRRLAILLTGALASAFAHADTVLDTVEVTARGNP
                     DGELVLPEAATPATLYRVDREAMRLFDTPGGCNPYTSVAEVPGVKVTPVDAWGLNNSQ
                     GGQKGMRVRGEVSTHGVSGTVEGLTLGGPGPGPGYLFLFDKENLRQVDFAQGAIAADR
                     GGLFNTSGALDSKLRWSQAKAGGEIGLSLGSDGFQRAFLRADSGRLASGTAFFFSASH
                     TQADKWRGHGEAPGDRENIELG"
     gene            1040..>2860
                     /locus_tag="PLN02_08035"
     CDS             1040..>2860
                     /locus_tag="PLN02_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI52311.1"
                     /translation="MKSEFADEKSVTRTHLLGTLGVIVVLTVALGAFFSWSSLAQHRQ
                     SLDRLQRVAAETQRQRLGSEMARALEHVEFARSRTESVLRQNLQAQVDGALQVAAAIH
                     ARESGRRPPAEVQRLIIEALRPVRFFDGRGYFFIDDMQGKFILLPTSPDLEGTTKLDN
                     QDDQGHYIMRGLIAAARQPAGLGFSRYRWYSPENPREMADKLAYVRHFAPYDWLIGTG
                     DYTHKWEEYQKGEALARLRELHFGESGHVAVVDAEGRILLAEDAASEGKRRSELSPLQ
                     AQAWTAILEARQNGWETVSYLWQRAVGGEAVSRTAQVRLVQPWGWTVVVAIDEDEMQR
                     ALAAEVERHVPIDGEKLGNLLLALGLALLLGLAASFCFSLWSNKIFRRYHRQNAAYAR
                     ELLESEAYSRILFAGSVVPMVVMEPDSGRFVDCNDAAVRIYGYSSREAVLGLTPVAVS
                     DVTQYDGSDSASAATRHIAACRAQGSVVFAWRHRRPDGTIWDADVTLFELPHNARRLM
                     LFQLQDVTERRNADAELARYRDHLEHEVAIRTEALTAAKDAAEAANVAKSAFLANMSH
                     EIRTPLNAITGMAYLIRRDGVGEKQADRLDKIDAAGRHLLD"
BASE COUNT          473 a          898 c          981 g          508 t
ORIGIN      
        1 accgagttcg atgttctcgc ggtcgcccgg cgcttcgccg tggccgcgcc atttgtcggc
       61 ctgggtgtgc gaggcggaga agaagaaggc ggtgccgctc gccaggcggc cgctgtcggc
      121 gcgcaggaaa gcgcgctgga agccgtcgct gcccaggctc aggccgatct cgccgccggc
      181 cttggcctgc gaccagcgca gcttgctgtc gagggcgccg gaagtgttga agagtccgcc
      241 gcggtcggcg gcgatcgcgc cctgcgcgaa atcgacctgg cgcaggtttt ccttgtcgaa
      301 caggaagagg tagccgggtc cggggccggg gccgccgagc gtgagccctt cgacggtgcc
      361 ggaaacgccg tgggtgctga cttcgccgcg cacccgcatg cccttctggc cgccttgcga
      421 attgttcagc ccccaggcgt cgaccggcgt gaccttgacg ccgggcactt cggcgacgct
      481 ggtgtaggga ttgcagccgc ccggcgtgtc gaacaggcgc atcgcctcgc ggtcgacccg
      541 gtacagcgtg gccggcgtcg ccgcttcggg caggacgagc tcgccgtccg ggttgccgcg
      601 ggcggtgact tccacggtgt cgagcacggt gtcggcgtga gcgaaggcgg aggcgagcgc
      661 cccggtcagc aagatcgcca gacggcgtcg ctgaaaaccg ctgttccctg aagattgcat
      721 ggcatttccc cttgatggat tttctggtct gatttgttat gtagttatat acataacgat
      781 aagtccagat cagcaattgc cgtgcccgcc tcaaatggtt gttatacggt ggataaggca
      841 ggagcgcttg tttcgttatg ttggaataaa tacaacgatg tcgggagtct gacaatccgt
      901 gtcgggaaac ggcgttgacc tgtcggcgat gcgccgacgg tctgttcgcc acggcgcccg
      961 gaaaattgcc ctgcgcaaag cgggccttcc cagtaccatt gttactcctt tagtaatagt
     1021 tggccgggcg ctcgttgtaa tgaaatccga attcgccgat gagaaatccg tcacccgcac
     1081 ccatctgctg gggacgctgg gggtcatcgt cgtgttgact gtcgcgcttg gcgccttctt
     1141 ttcctggagc agcctggccc agcaccggca gtccctggat cgcctgcagc gggtggcggc
     1201 cgagacgcag cgccagcgcc tgggcagcga gatggcgcgg gcgctcgaac atgtcgagtt
     1261 tgcccgttcg cggaccgaga gcgtcctgcg gcagaacctg caggcgcagg tcgacggcgc
     1321 gctgcaggtg gccgcggcga tccacgcccg ggaatcggga cgtcgcccgc cagccgaagt
     1381 gcagcgcctg atcatcgagg ccttgcgccc ggtacgtttc ttcgatgggc ggggctactt
     1441 cttcatcgac gacatgcagg gcaagttcat cctgctgccg acctcgcccg atctggaagg
     1501 gacgaccaag ctcgacaatc aggacgatca ggggcattac atcatgcgcg gcctgatcgc
     1561 ggcagcgcgc cagccggccg gcctgggctt ttcccgctat cgctggtatt ccccggaaaa
     1621 tccccgggaa atggccgaca agcttgccta cgtccgccat ttcgcgcctt atgactggct
     1681 gatcggcacc ggcgactaca cccacaagtg ggaggaatac cagaagggcg aggcgctggc
     1741 gcgcctgcgc gaactgcatt tcggcgagag cggtcacgtc gcggtggtcg acgccgaggg
     1801 gcgcatcctg ctggccgagg acgccgccag cgagggcaag cggcgcagcg agctttcacc
     1861 gctgcaggcg caggcctgga cggcgattct cgaggcccgg cagaatggtt gggaaaccgt
     1921 ttcctatctc tggcaacgcg ctgtcggcgg ggaagcggtg tcgcgcacgg cgcaggtcag
     1981 gctggtccag ccttggggat ggacggtggt ggtggcgatc gacgaggacg agatgcagcg
     2041 ggcgctggcc gccgaagtgg agcgtcatgt gccgatcgat ggcgagaagc tcggcaacct
     2101 gctgctcgcc cttggcctgg ccctgctgct cgggctggcg gcgtcgttct gcttttccct
     2161 gtggtcgaac aagatcttcc gccgctatca ccggcagaat gcggcctacg cccgggagtt
     2221 gctggaaagc gaagcgtaca gccggatcct gtttgccggt tcggtggtgc cgatggtcgt
     2281 catggaaccg gacagcgggc gcttcgtcga ttgcaacgac gcggcggtgc gcatttacgg
     2341 ctacagcagc cgcgaagcgg tgctcggcct gaccccggtc gccgtttccg acgtcacgca
     2401 atacgacggc agcgattcgg caagcgccgc cacccgtcac atcgccgctt gccgggccca
     2461 gggcagcgtc gtcttcgcct ggcgtcaccg gcgccccgac ggcacgatct gggatgccga
     2521 cgtcaccttg ttcgaactgc cgcacaacgc gcggcgactg atgttgttcc agttgcagga
     2581 tgtcactgag cggcgcaacg ccgacgccga actggcgcgt tatcgcgacc acctcgaaca
     2641 tgaggtggcg atccgtaccg aggccctgac cgcggcgaag gatgcggcgg aagccgccaa
     2701 cgtcgcaaag agcgccttcc tcgccaacat gtcgcacgag atccgcacgc cgctcaacgc
     2761 gatcaccggc atggcctatc tgatccgtcg cgatggcgtc ggcgagaaac aggcggaccg
     2821 tctggacaag atcgacgcgg cggggaggca tctgctggac
//
LOCUS       DAOSDX010000329         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12328_length_2858_cov_3.197645, whole genome shotgun sequence.
ACCESSION   DAOSDX010000329 DAOSDX010000000
VERSION     DAOSDX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12328_length_2858_cov_3.197645"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLN02_08040"
     CDS             complement(<1..372)
                     /locus_tag="PLN02_08040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI52312.1"
                     /translation="MKLDIAFVTETYPPEVNGVAMTVGRLVDGMRQRGHRVEVLRPRQ
                     GAADAGGEHDVPFAGVALPGYPGLQFGLPAGGTLRRRWRQRRPDLVHVVTEGPLGWSA
                     VAAARRLGIPVTSGFHTNFDRY"
     gene            complement(369..785)
                     /locus_tag="PLN02_08045"
     CDS             complement(369..785)
                     /locus_tag="PLN02_08045"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOI52313.1"
                     /translation="MRRPLPEELGLDPANESPFKGKTGLRRVWNAFRYSLDGLRAAYL
                     CEDAFRQETWLALLLIPLALWLPVGWLATALLIASLLLVLVVELLNSAIEAVVDRVGL
                     ENHRLAKRAKDIGSAAVFVSLCQVVVVWTGVLLEKL"
     gene            907..1353
                     /locus_tag="PLN02_08050"
     CDS             907..1353
                     /locus_tag="PLN02_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOI52314.1"
                     /translation="MHTRYLDDLSVGQIFNTAGVTLSQGEIIGFALQYDPQPFHLDAV
                     EAARSPYGGLIASGFQTLALCFRLFVQSGIFQSSSLGSPGIDELRWLAPVRPGDTLRT
                     EVEVLELRPSNSKPDRGIARLKFQARNQHGDAVIAFVVNLLLRRRA"
     gene            1436..1825
                     /locus_tag="PLN02_08055"
     CDS             1436..1825
                     /locus_tag="PLN02_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOI52315.1"
                     /translation="MESIMVFFELFAHNPTILRIPAGQTLFREGDADGGLMYVLSAGN
                     ADIVIGNRVVEHLQHGGIVGELGLVSPGPRSATVLAISDCEFVAIDEARFQFLIQNTP
                     FFAMQVMRVMAERLRATDRLMPVVEEV"
     gene            complement(1830..>2858)
                     /locus_tag="PLN02_08060"
     CDS             complement(1830..>2858)
                     /locus_tag="PLN02_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI52316.1"
                     /translation="VRVAGRPVVGDPDDRLAGWQAMHRAVFDAAADCDLIHVQTPFVA
                     HYAGLRAARRRALPVVSTYHTLFEEYLEHYAPWLPGGWLRAAARGFSRRQCNALDAVV
                     VPSTAMRQRLADYRVTVPTHVLPTGVPLAQFTGGDGAAFRAAHGIAAQRPVALFVGRV
                     AHEKNIEFLFHALREAQRRCPEILLVVAGEGPAMGELRARVAALGLAGAVRFIGYLDR
                     SQDLPNCYAAADAFVFASRTETQGLVLLEAMAAGLPVIALSAMGTADILENTAGCFTP
                     PDDPQAFGEVLGHVLANRDAWDYLGQDARRAAEAWSDSALAGRLAGLYRQLVAARQPQ
                     AVALAADT"
BASE COUNT          498 a          978 c          954 g          428 t
ORIGIN      
        1 gtagcggtcg aaattggtat ggaagccgga agtcaccggg atccccaggc ggcgcgcggc
       61 ggccaccgcc gaccagccga gcggcccctc ggtgaccacg tgcaccaggt ccggccgccg
      121 ttgccgccag cgccggcgca gggtgccacc ggctggcagg ccgaactgca ggccaggata
      181 accgggcagc gcaacaccgg cgaagggcac atcgtgctcc ccccccgcat ccgccgcgcc
      241 ttggcgcggt cgtagcacct cgacccggtg gccgcgctga cgcatgccat cgaccagacg
      301 gccgaccgtc atcgccacgc cgttgacttc cggcggatag gtttcggtga cgaaggcgat
      361 atccagtttc atagtttctc cagcaggacg ccagtccaga ccacgacgac ctggcacagc
      421 gagacgaaca cggcggcgct gccgatatcc ttggcgcgct tggccagccg gtggttctcc
      481 aggccgacgc ggtcgaccac ggcctcgatc gccgaattga gcaattcgac gaccagcacc
      541 agcagcaggc tggcgatcag cagcgcggtg gccagccagc cgaccggcag ccacagcgcc
      601 agcggtatca gtagcagggc cagccaggtt tcctggcgga aggcgtcttc gcacaggtag
      661 gcggcacgca ggccgtcgag cgaataacgg aaggcattcc agacccggcg caagccggtc
      721 ttgcccttga acggcgattc gttggcgggg tcgagtccga gttcctcggg cagtggtcgt
      781 cgcatcgcaa gttccctgaa atgcgaccaa ggtaatcggc cttcgctaag ctttcatgac
      841 aaccgccggt catcagggaa ttgggcgata ctggcgcccc aagccagcct cccgagagcc
      901 ccgaccatgc acacccgcta cctcgacgac ctcagtgtcg gccagatctt caacaccgcc
      961 ggcgtgacgc taagccaagg cgaaatcatc ggtttcgccc tgcagtacga tccgcagccc
     1021 ttccatctcg acgccgtcga agccgccaga tcgccctacg gcgggctgat cgccagcggc
     1081 ttccagacgc tggccctgtg tttccgcctg ttcgtgcaga gcggcatctt ccagtcatcg
     1141 agcctgggtt cgcccggcat cgacgaactg cgctggctgg cgccggtgcg tcccggcgac
     1201 acgctgcgca ccgaagtcga ggttctcgaa ttacgaccat cgaactccaa gccggaccgc
     1261 ggcatcgccc ggctgaaatt tcaggccagg aaccagcacg gcgacgcggt gatcgccttc
     1321 gtcgtcaacc tgctgctcag gcgccgggcc tgacgcggcc cgtcacggta acgcaagctg
     1381 cctgtaataa gcgggttatg accttcgtgc taaaaactgc ggtgatccca acctcatgga
     1441 aagcatcatg gtcttcttcg aactgttcgc gcacaacccc accatcctcc gcatccccgc
     1501 cggtcagacg ctgttccgtg aaggcgacgc ggatggcgga ctgatgtatg tcctgagcgc
     1561 cggcaatgcc gatatcgtca tcggcaaccg ggtcgtcgaa cacctgcagc atggcggcat
     1621 cgtcggcgaa ctgggcctgg tctcgccggg gccacgctcg gcgacggtgc tggcgatcag
     1681 cgattgcgag ttcgtcgcca tcgacgaggc gcgtttccag ttcctgatcc agaacacgcc
     1741 cttcttcgcg atgcaggtga tgcgggtgat ggccgaacgt ctgcgggcaa ccgaccggct
     1801 gatgccggtg gtcgaggagg tctgagttct caggtatccg ccgccagcgc caccgcttgc
     1861 ggctggcggg ccgcgacgag ctgccggtag agtccggcca ggcggccggc cagcgcgctg
     1921 tccgaccagg cctcggcggc gcgcctggcg tcctggccga gataatccca ggcgtcgcga
     1981 ttggccagga cgtggccgag cacttcaccg aaggcttgag gatcgtcggg cggcgtgaag
     2041 cagccggcag tgttttccag gatatcggcg gtacccatcg ccgacagcgc gatcaccggc
     2101 aggccggcgg ccatcgcttc gagcaggacc aggccctggg tttcggtgcg cgaggcaaag
     2161 acgaaggcgt cggcggcggc gtagcagttg ggcagatcct ggctacggtc caggtaaccg
     2221 atgaaacgga ccgccccggc aaggccgagt gccgcgacgc gggcgcgcag ttcgcccatc
     2281 gccggcccct cgccggcgac caccaggagg atctccgggc aacggcgttg cgcctcgcgc
     2341 agcgcgtgga agaggaattc gatgttcttc tcgtgggcga cgcggccgac gaacagcgcc
     2401 accggccgct gcgccgcgat gccgtgggcg gcgcggaaag cggcgccgtc gccgccggtg
     2461 aattgcgcca gcgggacgcc ggtcggcagc acgtgcgtgg gcaccgtcac gcgataatcg
     2521 gcaagccgct ggcgcatcgc cgtcgatggc acgacgacgg cgtcgagcgc attgcactgg
     2581 cgccgcgaaa aaccccgtgc cgcagcgcgc agccagccgc ccggcagcca gggggcgtaa
     2641 tgctcaaggt attcctcgaa cagcgtgtga taggtgctga ccaccggcag agcgcgacga
     2701 cgcgccgccc gcaggccggc gtagtgggcg acgaaaggtg tctggacatg gatcaggtcg
     2761 cagtcggcgg cggcgtcgaa caccgcgcgg tgcatcgcct gccatccggc caggcggtcg
     2821 tccggatcgc ccaccaccgg ccgtccggcg acccggac
//
LOCUS       DAOSDX010000330         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12438_length_2837_cov_3.150971, whole genome shotgun sequence.
ACCESSION   DAOSDX010000330 DAOSDX010000000
VERSION     DAOSDX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12438_length_2837_cov_3.150971"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLN02_08065"
     CDS             <1..390
                     /locus_tag="PLN02_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52317.1"
                     /translation="RLFENLAQRLLGQIEQVRDEVGHLKQARDALPPVKETAETNPNE
                     VLSYNDVEILRALAQEKFVYGNLAAVCERTGLPREYVSQRLTVMKTLGVIETRINDKN
                     VLHWFVSARGKAVLEEIVGNHQDKKLA"
     gene            431..1789
                     /gene="glmU"
                     /locus_tag="PLN02_08070"
     CDS             431..1789
                     /gene="glmU"
                     /locus_tag="PLN02_08070"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOI52318.1"
                     /translation="MNIVILAAGQGKRMHSNLPKVLHPIAGKPLAQHVIDTARQLAPE
                     KLIVVYGHGGEIVRATLLAPDILWAEQAQQLGTGHALAQAVPQLGDGAQTLVLYGDVP
                     LTSLATLKRLLQAGRDGLAILTVDLADPTGYGRIVRDEGGQVCRIVEQKDASEAEKAI
                     REINTGIMAMPTARLGEWLSSLKNDNAQGEYYLTDVVALAVAEGLPVRTAQPDGEWEV
                     LGVNSKVQLAELERQHQRNIADQLLVGGVRLADPARIDVRGQLAHGRDVAIDVGCVFE
                     GQVELGDAVEVGPYCVLKNVKVGAGTRIAAFCHFEDAVIGPDGVLGPYARLRPGTELG
                     PEVHVGNFVEIKKSVIGAQSKANHLAYIGDAEIGQRVNVGAGTITCNYDGANKFKTVI
                     EDDVFIGSDTQLVAPVTVHRGATLGAGTTLTKDAPPDALTVSRPKQITVAGWQRPVKE
                     KK"
     gene            1789..2112
                     /locus_tag="PLN02_08075"
     CDS             1789..2112
                     /locus_tag="PLN02_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52319.1"
                     /translation="MDGWFVAFAIVSSLLMAGGGTLLLVGYINTLPAALSFGWRTALP
                     VVVLPVVGTLWFAWTQGEEFRRARYQLIAALALLAAAGVLILAFGPYFAGRLIAEMVE
                     AAKMR"
     gene            2146..>2837
                     /locus_tag="PLN02_08080"
     CDS             2146..>2837
                     /locus_tag="PLN02_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOI52320.1"
                     /translation="MSKFTSESLRSVVLVGHGAAGKTTLAEALLHATGAIAAKGSIER
                     GSTVCDSDPQEKETGHSLSSAVFSIRHEGITIHAVDTPGYPDFAGQAIAALAAADTAL
                     VVINAQAGIELTAERMMRLAGERGLCRMIVVNKIDADNLDLPGLVAEIRERFGSQCLV
                     LDLPAHGAADVVEVLEHDAGEADFASVAAAHRALIDQIVEEDEDLLARYLDEGSDPST
                     NELHSPFEKALRA"
BASE COUNT          521 a          921 c          912 g          483 t
ORIGIN      
        1 cgtctgttcg agaacctggc gcagcgtctc ctcggccaga tcgagcaggt ccgggacgag
       61 gtcggccacc tcaagcaggc gcgcgacgcc ttgccgccgg tgaaggagac ggccgagacc
      121 aaccccaatg aagtgctctc ctacaacgat gtcgagatcc tgcgggcgct ggcccaggag
      181 aagttcgttt acggcaatct ggctgccgtc tgcgaacgca ccggtttacc ccgggaatac
      241 gtcagccagc gtctgaccgt gatgaagacg ctgggcgtca tcgaaacccg gatcaacgac
      301 aagaacgtcc tgcactggtt cgtctccgcg cgtggcaagg ccgtgctcga ggaaatcgtc
      361 ggcaaccacc aggacaagaa actcgcctga gatcaacgcc ggatgcccgg ccattacctg
      421 aaaaatcacc atgaacatcg tcatcctcgc tgccggtcaa ggcaagcgca tgcattcgaa
      481 tttgcccaaa gtgctgcatc cgatcgccgg caagccgttg gcccagcacg tcatcgatac
      541 cgcgcgccag ttggcgccgg aaaaactgat cgtggtttac ggccacggtg gtgaaatcgt
      601 ccgggcgacg ctgttggctc ccgacatcct gtgggccgaa caggcacagc aactgggcac
      661 tggccacgca ctggcacagg ccgtcccgca actgggcgac ggggcacaga cgctggtcct
      721 ctacggcgat gtgccgctga cctcgctggc aaccctgaaa cggctgctgc aagctggccg
      781 cgacgggctg gccatcctca ccgtcgatct cgccgatccc accggctacg gccgcatcgt
      841 ccgcgacgag ggcggccagg tctgccgcat cgtcgaacag aaggatgcga gcgaggccga
      901 gaaggcgatc cgcgaaatca ataccggcat catggcgatg ccgaccgcaa ggctgggcga
      961 atggttgtcc agcctgaaga acgacaacgc ccagggcgag tactacctga ccgacgtcgt
     1021 cgcgctggcg gtcgccgaag gcctgccggt gcgtaccgcg cagccggatg gcgagtggga
     1081 agtgctcggg gtcaacagca aggtgcaact ggccgagctg gaacgccagc atcagcgcaa
     1141 catcgccgat caactgctgg tcggcggcgt ccgtctggcc gatccggcgc gcatcgacgt
     1201 ccgcggccag cttgcccacg ggcgcgacgt ggcgatcgac gtcggctgcg tttttgaagg
     1261 ccaggtcgaa ctgggcgacg cggtcgaagt cggcccttac tgcgtgctga agaacgtcaa
     1321 ggtcggcgcc gggacacgga ttgccgcctt ttgccacttc gaggacgcgg tgatcggtcc
     1381 ggacggcgtg cttggcccct acgcccgtct gcggccgggc accgaactcg gtcccgaggt
     1441 ccatgtcggc aacttcgtcg agatcaagaa gagcgtcatc ggtgcccagt cgaaggcgaa
     1501 tcacctggcc tacatcggcg acgccgagat cggccagcgg gtcaacgtcg gcgccggcac
     1561 catcacctgc aactacgatg gcgccaacaa gttcaagacc gtgatcgagg acgacgtttt
     1621 tattggctcc gatacccaac tggtggcgcc ggtcaccgtc catcgcgggg cgaccctcgg
     1681 tgccggcacg acgctgacca aggatgcgcc gcccgacgca ctgaccgtgt cgcgtcccaa
     1741 gcaaatcacg gtggctggct ggcagcgtcc ggtcaaggag aagaagtgat ggatggctgg
     1801 ttcgtcgcct tcgcgatcgt cagttcgctg ctcatggccg gcggtggtac cttgctgctg
     1861 gttggctaca tcaacacctt gccggcggca ctgagtttcg gttggcgcac cgccttgccg
     1921 gttgtcgtgc tgccggtggt cggcacgctg tggttcgcgt ggacccaggg cgaggaattc
     1981 cgccgggcgc gctaccaatt gattgccgcg ctggccctgc tggccgctgc cggcgtcctg
     2041 attcttgctt ttggccctta ttttgcggga cgtttgatcg ccgaaatggt cgaggcggcg
     2101 aaaatgcgtt agcgttgtac cgacaatttg ctcagggaga agaagatgtc caagttcacc
     2161 agtgaatccc ttcgtagtgt cgtcctcgtc ggccacggtg ccgctggcaa gaccacactg
     2221 gccgaggcgc tgctccacgc taccggcgcc atcgcggcga aaggcagcat cgagcgcggc
     2281 agcaccgtct gcgacagcga cccgcaggaa aaggaaaccg gccattccct gagcagtgcg
     2341 gttttttcga tccgccacga aggcattacc atccacgctg tcgatacccc gggctacccg
     2401 gatttcgccg gtcaggcgat tgccgcgctg gccgccgccg ataccgcttt ggtcgtgatc
     2461 aatgcccagg caggcatcga actgaccgcc gaacggatga tgcgcctggc cggcgaacgc
     2521 ggcctgtgcc ggatgatcgt ggtcaacaag atcgatgccg acaaccttga cctgcccggt
     2581 ctggtggccg agattcgcga gcgtttcggc agccagtgcc tggtgctcga cctgccggcg
     2641 catggcgccg ccgacgtcgt cgaggtcctc gagcacgatg ccggcgaggc cgattttgcc
     2701 tccgtcgccg ccgcccaccg ggcgctcatc gaccagatcg tcgaggagga cgaggacctg
     2761 ctcgcccgct acctcgacga aggcagcgac ccgtcgacga acgagctgca ctcgcccttc
     2821 gagaaggcct tgcgcgc
//
LOCUS       DAOSDX010000331         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12442_length_2836_cov_3.856526, whole genome shotgun sequence.
ACCESSION   DAOSDX010000331 DAOSDX010000000
VERSION     DAOSDX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12442_length_2836_cov_3.856526"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2836
                     /locus_tag="PLN02_08085"
     CDS             <1..>2836
                     /locus_tag="PLN02_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOI52321.1"
                     /translation="LRDPAGNLSPTGSDAVTVDTTVYKNLAVEITEDRNDDGFISIAE
                     LQGNDIDVRVTLPAGAAVGDSLTVSGSGNVDQVITLTAAHLAAGHVDLKFNPTADNTD
                     FVATARITDIAGNAAGPVSDSARLLLSEPGAPIVAITEDADNNRWISAAELNGDIDVS
                     ITLPGTARVGDSLLVSINGVAQAPHVLTLADINRGSIALAVANPGEGQSLEVTAQVRD
                     PAGNLGKIGSDSATVDTIPPNGGKAPTVTITEDANNDGYINAAELNGDVDVQIAFDPA
                     KVDAGDIVVVSSGTITREITVTAADKANGYVSTAFPPQAHGTTLTVEALIRDPAGNAT
                     PRGSDSAEFRTALIIDDLTVSVSEEGLAKGLPDSLGLPTDSTDSTVVSGSLVVANGTP
                     ALSYTLLAPSEELCSNGVRIVWSGDGTEASPLVGRAGLLGPEVICAKVDAQGNYEIRL
                     LQAVDHLDTNGEDIAALNFTVQASDGTQQHSATLTLNIEDDAPLARDQSFRVGDGDGA
                     DTNLMIVLDISSSMNECACVKDLAGNDLSRLDLAKLAINDLLDRYDGLGDVMVRLVIF
                     GNDGQAIGDRWLTVDEARAALAGISVSGEASNYDAALATASQAYADPGRLATAQNVSY
                     FFSDGEPTTDSSGHPFNYSGQFDPFLGDGIDQGEELAWQAFLAQHQIVSQAIGVGPRG
                     VIYPDQLSPIAWNGAVGCDLNSNWIGDFNEMACVFSNTVSGPTTLTGSLGGFGADGGH
                     VASLSIGGYSFAYDVQTNTLTASGSSDSVTAYQFDPVARTLTLTTVQGETLRIGLCDG
                     SFHYHVDETPVRGSHTDLQYTLVDGDGDGDSASGSLGFVGTGAPANVAPVAGIETGNN
                     LLGIIGLEALDLIDLGTRTRFTALDANNDLMRVEIAYRALVNLGPYHLQASLALAAEL
                     GLHVEITNDPGLLGLVLPSSTLVITAADGGS"
BASE COUNT          516 a         1111 c          831 g          378 t
ORIGIN      
        1 gttgcgcgat cccgccggca acctcagccc gaccggcagc gatgccgtca ccgtcgatac
       61 cacggtctac aagaatctgg ccgtcgaaat cacggaagac cgcaacgacg acggcttcat
      121 ctcgatcgcc gaactgcagg gcaacgatat cgacgtccgc gtcacgctgc ccgccggcgc
      181 cgccgtcggc gattcgctga ccgtctccgg cagcggcaac gtcgatcagg tgatcaccct
      241 gaccgccgcc catctcgccg ccggtcacgt cgatctcaag ttcaacccga ccgccgacaa
      301 caccgacttc gtcgccaccg cccgcatcac cgacatcgcc ggcaacgctg ccggcccggt
      361 cagcgacagc gcccgcctgc tgctctccga acccggcgcc cccatcgtcg cgatcaccga
      421 ggacgccgac aacaaccgct ggatcagtgc cgccgaactc aacggcgaca tcgacgtcag
      481 catcaccctg cccggcaccg cccgcgtcgg cgacagtctg ctcgtctcga tcaacggcgt
      541 cgcccaggcg ccgcatgtgc tgacgcttgc cgacatcaac cgcggcagca tcgccttggc
      601 cgtcgccaat cctggtgaag gacaaagcct tgaagtcacc gcccaggtcc gcgacccggc
      661 cggcaatctc ggcaagatcg gcagcgacag cgccaccgtc gataccatcc cgcccaacgg
      721 cggcaaggca ccgaccgtca ccatcaccga agacgccaat aacgacggct acatcaacgc
      781 cgccgaactg aacggcgacg tcgatgtgca gatcgccttc gacccggcca aggtcgacgc
      841 cggcgatatc gtcgtcgtca gcagcggcac gatcacccgg gaaatcaccg tcaccgccgc
      901 cgacaaggcg aacggttacg tcagcaccgc cttcccgccg caagcgcacg gcacaacact
      961 gaccgtcgag gcgctgatcc gcgatccggc cggtaacgcg acgccgcgcg gcagcgacag
     1021 cgccgaattc cgcaccgcgc tgatcatcga cgacctgacc gtcagcgttt ccgaggaagg
     1081 cctggccaag ggcctgcccg atagcctcgg cctgcccacc gacagcaccg actcgacggt
     1141 cgtcagcggc tcgctggtcg tcgccaacgg caccccggcg ctcagctata ccctgctggc
     1201 gccgagcgag gaactgtgct cgaacggcgt gcgcatcgtc tggtccggcg acggcaccga
     1261 ggcctcgccg ctggtcggcc gcgccggcct gctcggcccg gaagtcatct gcgccaaggt
     1321 cgacgcccag ggcaactacg aaatccgcct gctgcaggcg gtcgaccacc tcgacaccaa
     1381 cggcgaggac atcgccgcgc tcaacttcac ggttcaggcc agcgacggca cccagcagca
     1441 cagcgcgacg ctgaccctga acatcgagga cgacgcgccg ctcgcccgcg accagagctt
     1501 ccgcgtcggc gatggcgacg gcgccgacac caacctgatg atcgtcctcg acatctcgtc
     1561 gagcatgaac gaatgtgcct gcgtcaagga cctggccggc aacgacctga gccgcctcga
     1621 cctcgccaag ctggcgatca acgacctgct cgaccgctac gatggcttgg gcgacgtcat
     1681 ggtccgcctc gtcattttcg gcaacgacgg ccaggcgatc ggcgaccgct ggctgaccgt
     1741 ggacgaggcg cgtgccgcgc tggccggcat cagcgtcagc ggcgaagcgt cgaactacga
     1801 cgcggcactg gccaccgcca gccaggccta cgccgacccc ggccgcctcg ccacggcgca
     1861 gaacgtctcc tacttcttct ccgacggcga gccgaccacc gacagcagcg gccacccatt
     1921 caactacagc ggccagttcg accccttcct cggcgacggc atcgaccagg gcgaggaact
     1981 cgcctggcag gctttcctgg cgcagcacca gatcgtctcg caggcgatcg gcgtcggccc
     2041 gcgcggcgtc atctaccccg accagctcag cccgatcgcc tggaacggcg ccgtcggctg
     2101 cgacctgaac agcaactgga tcggcgactt caacgagatg gcctgcgtct tctccaacac
     2161 cgtcagcggc ccgaccaccc tcaccggcag cctcggcggc ttcggcgctg acggcggcca
     2221 cgtcgccagc ctgagcatcg gcggttacag cttcgcttac gacgtgcaga ccaacacgct
     2281 gaccgccagc ggcagttcgg acagcgtcac cgcctaccag ttcgacccgg tggcgcgcac
     2341 cctgacgctg accacggtgc agggcgagac gctgcgcatc ggcctctgtg acggcagctt
     2401 ccactaccac gtcgacgaaa caccggtacg cggcagccac accgacctcc agtacacgct
     2461 ggtcgatggc gacggcgacg gcgacagcgc cagcggctcg ctcggcttcg tcggcaccgg
     2521 cgccccggcc aacgtcgcgc cggtggccgg catcgagacc ggcaacaacc tgctcggcat
     2581 catcggcctc gaagcgctcg acctgatcga cctcggcacc cgcacccgct tcaccgcgct
     2641 ggacgccaac aacgacctga tgcgcgtcga gatcgcctac cgggcgctgg tcaatctcgg
     2701 cccctaccat ctccaggcct cgctcgcgct ggccgccgaa ctcgggctgc acgtcgagat
     2761 caccaacgat cccggcctgc tcggcctggt cctgcccagc tcgacgctgg tcatcaccgc
     2821 cgccgacggc ggcagc
//
LOCUS       DAOSDX010000332         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12451_length_2835_cov_2.731655, whole genome shotgun sequence.
ACCESSION   DAOSDX010000332 DAOSDX010000000
VERSION     DAOSDX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12451_length_2835_cov_2.731655"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLN02_08090"
     CDS             <1..677
                     /locus_tag="PLN02_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HOI52322.1"
                     /translation="ATCVGWGPIGDAGYLTRNQAVKDSLASRLGAAPLAVETALAMLG
                     EVLAAGSPNLALADFHWPTLARLLPSSHSPRFAALWRNGDEAASGPENLEDFRSLIEG
                     KSPDEVRQLVRSLVTQEVAQTLAINPERIDAGRSLHDLGLDSLMGVELALGLEKRFGV
                     QVPAMMLNEGPTVDRVTERIVERLGGGGEVNDLASTAAGMAAQHAQDIAPEILESVVN
                     DLQGKA"
     gene            930..1976
                     /locus_tag="PLN02_08095"
     CDS             930..1976
                     /locus_tag="PLN02_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI52323.1"
                     /translation="MLNFSSYNYLGLSGDSRVSAAVKAAIDRYGTSLAAAPRVAGEVA
                     VVRELETAIAGLYGVDDAVTFASGHATNVNTIGFLFGPKDLIVHDQLVHNSVIEGVRL
                     SGATRRAFPHNDPAALDRLLTEIRGNFERVLVVIEGLYSMDGDSPALASFVEIKRRHR
                     AFLMVDEAHSLGVLGSSGRGIHEHCGVPAGDVDIWMGTLSKSFAGCGGFIAGEQALVD
                     LIRYAAPGYNASVGLAPALAAASSAVIRIMLDEPWRLEQLRAAGRYFLEQARLRGMNT
                     GLSEGYCVVPLITGSSLKAVSLSKKLARQGISVQPIIHPAVEERLARLRFFICYAHTQ
                     TQIDHTLDEIARCR"
     gene            1993..>2835
                     /locus_tag="PLN02_08100"
     CDS             1993..>2835
                     /locus_tag="PLN02_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI52324.1"
                     /translation="MSVLAGDLREKLVKRFAARRETGEVTSAPSPSAASPDIDQFCRF
                     DAFPAYRQIVSSLQSSQPALAPGQGEGDFASYDYLGLASDPRVREAAKQAVDQYGSSV
                     SASRLVAGERPLLRELEAALAEIYAVDDALVFVSRYAGNETTIGHLFGPRDLVVHDQF
                     CHLSVLEGVRLAGATRRAFPHNDLPALEKLLAGARGDYERVLIVADGLSPVEGDICDL
                     PGLVALKQRYRTFLMVDDAHALGVLGEGGRGSREHWALAGDDVDFWSGSLGHALAGAG
                     GYIAG"
BASE COUNT          485 a          883 c          936 g          531 t
ORIGIN      
        1 cggcgacctg cgtcggctgg gggccgatcg gcgatgccgg ttacctgacc cgcaaccagg
       61 cggtcaagga cagcctcgcc agccgtctcg gcgcggcgcc gctggcggtg gaaacggcgc
      121 tagccatgct cggcgaagtg ctcgccgccg gcagccccaa tctggcgctg gccgatttcc
      181 actggccgac gctggcgcgt ctgctgccgt ccagtcacag cccgcgtttc gccgcgctct
      241 ggcgcaacgg cgacgaagcg gcgagcggcc cggagaacct cgaggacttc cgcagtctga
      301 tcgaaggcaa gtcgccggat gaggtcaggc aactggtgcg ttcgctggtc acccaggaag
      361 tcgcgcagac gctggcgatc aaccccgagc gcatcgacgc cggtcgttcg ttgcacgacc
      421 tcggcctcga ttcgctgatg ggcgtcgaac tcgctctcgg cctggaaaag cgcttcggcg
      481 ttcaggtgcc ggcgatgatg ctgaacgagg ggccgaccgt cgatcgcgtc accgagcgca
      541 tcgtcgagcg tctcggcggt ggtggcgagg tcaacgatct tgccagcacg gcggccggca
      601 tggcggcgca gcatgcgcag gatattgcac cggaaatcct ggagtcggtg gtcaatgatt
      661 tgcaggggaa agcctgaccc atggcaattt tctcggcggg gcgcagcaag gagacagcca
      721 tccatttggc caaaaatacg ggcgaaaggc cgatggtggc tgatggcgga ctcgacgtac
      781 cggaaaagtt ctatcggatc gacaagtttc ccggctatac caaaatcgca ttgtttcgtc
      841 ttgcagcgga aaagttgcgg atcgacgacc cttacttcat ttgccatgag ggcgtggcag
      901 gcgcgacgac gacggttggc ggacggagca tgctcaattt ttcgagctac aactacctcg
      961 gcctttccgg cgattcccgc gtcagtgccg cagtcaaggc ggcgatcgac cgttacggta
     1021 cctcactggc ggccgcgccc cgggtggccg gcgaggtggc cgtggtgcgc gaactggaaa
     1081 ccgcgatcgc cggcctgtac ggcgtcgatg acgcggtgac cttcgccagc ggccacgcca
     1141 ccaacgtcaa taccatcggt ttcctgttcg gaccgaagga tttgatcgtt cacgaccagt
     1201 tggtccataa cagcgtgatc gaaggtgtcc gtctttcggg agcgacgcgc cgcgcctttc
     1261 cccataacga tcccgccgcg ctggatcgcc tgcttaccga aatccggggc aacttcgagc
     1321 gggtgctcgt ggtgatcgaa gggctctaca gcatggacgg cgattcgccg gcgttggcga
     1381 gctttgtcga aatcaagcgg cggcaccgcg ccttcctgat ggtcgacgag gcgcattcgc
     1441 tcggtgtgct gggtagcagc ggtcggggta ttcacgaaca ctgcggcgtg ccggctggtg
     1501 acgtcgacat ctggatgggt accctgagca aatcgttcgc cggctgcggc ggtttcatcg
     1561 ctggcgagca ggcgctggtc gacctgatcc gctacgcggc accgggctac aatgccagcg
     1621 tcggtctggc gccggcgctg gcggcagcat catcggcggt catccggatc atgctcgatg
     1681 agccctggcg gctggaacag ttacgtgcgg cgggccgcta ttttctcgaa caggctcgac
     1741 tccgtggcat gaataccgga ctctccgagg gctactgcgt cgttcctctg attactggca
     1801 gttcgctgaa ggcggtcagt ctctcaaaga aacttgctcg gcaaggaatc agcgtgcaac
     1861 cgatcatcca tccggcggta gaggagaggc tggcgcgcct gcgtttcttc atctgttacg
     1921 cccatacgca gacccagatc gatcacacac tcgacgagat tgcccgttgc cgctgaaacc
     1981 atgaaaaagc ccatgtccgt tctcgccggc gatcttcggg agaaactcgt caaacgcttt
     2041 gccgcccgcc gggaaaccgg cgaagttacg tccgcgccaa gtccgtccgc tgcgtcaccg
     2101 gatatcgacc agttctgtcg cttcgatgcc tttcctgcct atcggcagat cgtctcgtcg
     2161 ctacagtcca gccaaccagc gcttgctccg gggcagggag aaggcgattt cgcgagctac
     2221 gactatctgg gcctggccag cgacccgcgc gtccgggaag cggccaagca ggccgttgat
     2281 caatacggca gttcggtctc ggccagtcgt ctggtggccg gcgagcgccc cttgctgcgc
     2341 gaactcgaag cggcgctggc ggaaatctac gcagtggacg atgcgctggt ttttgtcagc
     2401 cgttatgccg gcaacgaaac gacgatcggc catctgttcg gcccgcggga tcttgtcgtc
     2461 catgaccagt tctgtcatct cagcgtactt gagggcgtcc gtctggccgg cgcgacgcgg
     2521 cgggcctttc cgcacaacga cttgccggcg ctggaaaaac tgctcgccgg agcgcgcggc
     2581 gattacgagc gggtgctgat cgtcgccgac gggctatccc cggtcgaagg cgatatctgc
     2641 gacttgccgg gcctggttgc cctgaaacag cgctaccgga ccttcctgat ggtcgatgac
     2701 gcccatgcgc tgggcgtgct cggcgaaggc ggccgcggca gccgcgagca ctgggcgctg
     2761 gccggcgacg atgtcgattt ctggtcgggc agcctcgggc atgcactggc cggggcgggc
     2821 ggttatatcg ccggc
//
LOCUS       DAOSDX010000333         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12494_length_2828_cov_3.391273, whole genome shotgun sequence.
ACCESSION   DAOSDX010000333 DAOSDX010000000
VERSION     DAOSDX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12494_length_2828_cov_3.391273"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1643
                     /locus_tag="PLN02_08105"
     CDS             375..1643
                     /locus_tag="PLN02_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOI52325.1"
                     /translation="MQAKLSAVALSIAAAFALSACGPKEEAPPPVAAPAAPAAKPEVV
                     VKLGHVAPMTGPQAHLGKDNESGAVLAVEELNAQGLEIGGAKVKFQLVSEDDQADPKQ
                     GTIVAQKLVDEKVNGVVGHLNSGTTIPASKLYADAGLPQVSPSATNPKYTQQGFKTAF
                     RVMANDVQQGKVLGEFAAKQGVKTVAIIDDRTAYGQGLADEFRKAAEGLGLKVVANEY
                     TTDKATDFKAILTKIKSTKADLVFYGGMDAQGGPMVKQMKELGIKAKFLGGDGVCTPE
                     FMKLGGPAADGQFCSLPGVPLEQLAKGPEFKTKFNTRFGYDIQLYAPNAYDAVMVIAD
                     AMKRADSVEPAKYLPEVGKTNLEGVTGKIEFDEFGDLKSGAISLYQYKGGTLSYVETL
                     GGAPAPEAAPAPAEPAAAPAAPAAPAEAKK"
     gene            1714..2661
                     /locus_tag="PLN02_08110"
     CDS             1714..2661
                     /locus_tag="PLN02_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI52326.1"
                     /translation="MDISLHQLLQQFPQQVVNGLVQGSIYALVALGYTMVYGIMGLIN
                     FAHGEVVMIGTLVAITVTGLLAAAGMPVGIAGLCGLVISILSCMAIGWSLERVAYRPL
                     RGSPRLTPLITAIGMSIVLQNLAMILWGRNYMTFPPLLPKVNFEFAGAYFTLVQVVIV
                     LLSALMMSGLVLLVYRTKLGMAMRATAQNPEVASLMGVNINRVISAAFVIGSALAAVA
                     GVMVGSYYEVAHYQMGFVLGLKAFTAAVLGGIGNLVGAMVGGILLGLIEALGAGYIGY
                     FTGDFLGSHYQDIFAFAVLIVVLMFKPSGLFGEKTGDRA"
BASE COUNT          529 a          883 c          842 g          574 t
ORIGIN      
        1 atgattccaa atgttgcact cggtctttga aaccgccgct actttctttt ttccaaaaaa
       61 agaaagtacg cccgccggtc agcggcggaa aacggcgcat caataaccaa agccgctcgc
      121 cgaagcataa gagaacccag cggttcagca tcaacaaacg cccgcctggc aaaagtggac
      181 cccgccgcct cggaaaccaa tcccgcccga atatcagcgc ggataacccc gcttctcggc
      241 gacaatcagt ccaaacgcgc cagccgccgc gattttaggg atattccgta ttgacgatgc
      301 gctatctgta tgcgcagaat cggtccctgt tgtttcgagc agatttcccg ccccccaatt
      361 caggagactt accgatgcaa gccaagcttt ccgcagtagc cctctccatt gccgccgctt
      421 tcgcgctgtc cgcttgcgga ccgaaggaag aggcgccgcc gccggtcgcc gcgcccgccg
      481 cacccgccgc caagcccgaa gtcgtcgtca agctcggcca tgtcgcgccg atgaccggcc
      541 cgcaggccca ccttggcaag gacaacgaga gcggtgccgt tctcgccgtc gaggagctga
      601 atgctcaggg tctcgaaatc ggtggcgcca aggtcaagtt ccagttggtc tcggaagacg
      661 accaggccga tccgaagcag ggcaccatcg ttgcccagaa gctggttgac gaaaaggtca
      721 atggcgttgt cggccacctt aactccggta cgacgatccc ggcttccaag ctgtacgcgg
      781 atgctggcct gccgcaggtt tcgccgtcgg cgaccaaccc gaagtacacc caacaaggtt
      841 tcaagacggc tttccgcgtg atggccaacg acgtccagca gggcaaggtg ctcggcgagt
      901 ttgccgccaa gcagggcgtc aagaccgtcg ccatcatcga cgaccgtacc gcttacggtc
      961 agggtctggc cgacgagttc cgcaaggcgg ccgaaggcct gggcctgaag gtggtggcta
     1021 acgaatacac caccgacaag gcgaccgact tcaaggcgat cctgaccaag atcaagtcga
     1081 ccaaggccga tctggtcttc tacggcggca tggatgcgca gggcgggccg atggtcaagc
     1141 agatgaagga actcggcatc aaggccaagt tcctcggcgg cgatggcgtc tgcacgccgg
     1201 agttcatgaa gctgggcggc ccggctgccg acggtcagtt ctgctcgctg ccgggcgtgc
     1261 cgctggaaca attggcgaag ggtccggaat tcaagaccaa gttcaacacc cgctttggct
     1321 acgacatcca gctctacgcg ccgaacgcct acgatgcggt gatggtgatt gccgatgcga
     1381 tgaagcgtgc tgattcggtc gagcccgcca agtacctgcc ggaagttggc aagaccaacc
     1441 ttgaaggcgt taccggcaag atcgagttcg acgaattcgg cgacctgaag agcggcgcaa
     1501 tttcgctcta ccagtacaag ggcggcacgt tgagctatgt cgaaaccctg ggtggtgccc
     1561 cggctccgga agctgccccg gctccggctg agcccgccgc cgcgccggcg gcccccgcgg
     1621 ccccggccga agcgaagaag taagcagcct gtctgctcaa tgaaacggca ccgcaaggtg
     1681 ccgtttccgt atcttgcagt caatggattc caaatggata tttcccttca tcagctcctc
     1741 cagcaatttc cgcagcaagt tgtcaatggt cttgtccagg gcagtatcta cgcgctggtc
     1801 gcgctgggct acacgatggt ctacggcatc atgggcctga tcaattttgc gcacggcgag
     1861 gtggtgatga tcggtaccct ggttgccatc accgtcaccg gtttactggc cgccgccggc
     1921 atgccggtcg gcatcgccgg cctgtgcggt ctggtcatct ccatcctttc ctgcatggcg
     1981 atcggctgga gcctggagcg ggtggcctac cgccccttgc gcggttcgcc gcggctgacg
     2041 ccgctgatta ccgccatcgg catgtcgatc gtgctgcaga acttggcgat gattctctgg
     2101 ggacgtaact acatgacctt tcccccgctt ttgcccaagg tcaatttcga attcgcaggc
     2161 gcctacttca ctttggtcca ggtggtcatc gtgttgcttt cggccctgat gatgagcggt
     2221 ctggtgctgc tcgtctatcg caccaagctg ggcatggcaa tgcgtgccac ggcgcagaac
     2281 cccgaggtgg ccagcctgat gggggtgaac atcaaccggg tgatctcggc ggctttcgtc
     2341 atcggctcgg cgctggcggc ggtagctggc gtgatggtgg gcagctacta cgaagtggcg
     2401 cattaccaga tgggtttcgt cctcggcctg aaggccttca ccgctgccgt tctcggtggc
     2461 atcggcaacc tcgtcggggc gatggtcggc ggcatcctgc tcggcttgat tgaagcactc
     2521 ggcgctggtt atatcggtta tttcactgga gattttctcg gtagtcacta tcaggacatc
     2581 tttgccttcg cggtcctgat cgtcgtgctg atgttcaagc cctccggcct gttcggtgaa
     2641 aagacggggg atcgggcatg aagaacttgc taagcccccg ttccaccctg gggatcgtgc
     2701 tcatcgccgc cgccctgatc gccttgccct tcgtcgtcgg catggccggt caggcctggg
     2761 tgcgcatcct caacttcgcc atcctctaca tcttcctggc gcttggcctg aacatcgtcg
     2821 tcggtttt
//
LOCUS       DAOSDX010000334         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12496_length_2828_cov_2.674360, whole genome shotgun sequence.
ACCESSION   DAOSDX010000334 DAOSDX010000000
VERSION     DAOSDX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12496_length_2828_cov_2.674360"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..871)
                     /gene="rpoH"
                     /locus_tag="PLN02_08115"
     CDS             complement(11..871)
                     /gene="rpoH"
                     /locus_tag="PLN02_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HOI52327.1"
                     /translation="MTHALALPVPSAVGNLDAYIQAANRYPLLSEAEETRLAERFHND
                     GDVDAARQLVLSHLRLVISIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPSRGVRLV
                     SFAMHWIKAEIHEYILKNWRLVKVATTKAQRKLFFNLRSMKADYEGVDTLSGRQAEEV
                     AQRLGVKRDEVVEMETRLSGRDLALEGNPDDGEDSFAPIDYLADSRYEPTRMLENKAQ
                     ARLQDEGLHQALAALDPRSRRIVEARWLADGEASTLHELAAEFDVSAERIRQIEAKAL
                     QKMRGLLAAA"
     gene            978..1904
                     /gene="gluQRS"
                     /locus_tag="PLN02_08120"
     CDS             978..1904
                     /gene="gluQRS"
                     /locus_tag="PLN02_08120"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOI52328.1"
                     /translation="MFVTGALLYRGRFAPSPTGPLHFGSLIAALASCLEARCRGGEWL
                     LRIEDVDTPRNVPGAADAILRTLEAFGFVWDGPLLYQSRRVAAYEAALDVLRGQGLLY
                     PCACSRSEIAAAASRTAVDGGAVYPGTCRDGLRPGAEARAWRLRVDDAVVVFEDRLQG
                     IVEQRLAEQVGDFVLRRADGIHAYQLAVVVDDASQGITDIVRGADLLASTPRQLWLQQ
                     CLGYPRPGYAHLPVATNPAGEKWSKQTLAPALAAGKAAAELVRALRFLGQPAPAELAA
                     APVAEVWDWALANWSFATIPRVPAISVPATAA"
     gene            complement(1879..>2828)
                     /gene="clsB"
                     /locus_tag="PLN02_08125"
     CDS             complement(1879..>2828)
                     /gene="clsB"
                     /locus_tag="PLN02_08125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HOI52329.1"
                     /translation="AAAGVRALFYRPEIRRFRLQRHRLRRLHRKLAVIDGTIAFVGGI
                     NIVDDDNAPAEMRPRYDYAVRVEGPLLAPIHHAVRRMWEIVTWVNFRRRFRLAPLLPP
                     CCTSAGEQTAAFLIRDNIRHRNDILNAYIDAIEAAQEEILIANAYFLPGIRFGRALYA
                     AARRGVRIDILLQGKTDHPLLRYATQTLYASALAAGIRIFEYEKSFLHAKVAVIDHRW
                     ATVGSSNIDPFSLLLAKEANLVARDRAFAEELRASLYEAIDTGSRELRAQDIAGQPWH
                     SRFLRWLSYGLVRLLVGIAGYGDRHWRGGEPVKPQSPEH"
BASE COUNT          423 a          857 c         1030 g          518 t
ORIGIN      
        1 gaagatgcgg tcaggcggcg gcgagcagtc cgcgcatctt ctgcagggcc ttggcttcga
       61 tctgccggat acgttcggcg gaaacgtcga attcggcggc cagttcgtgc agtgtcgagg
      121 cttcgccgtc ggccagccag cgggcttcga cgatgcgccg gctgcgcggg tcgagggcgg
      181 ccagcgcctg gtgcaggcct tcgtcctgca ggcgggcctg ggccttgttc tcgagcatcc
      241 gggtcggctc gtagcggctg tcggccaggt agtcgatcgg cgcgaagctg tcctcgccgt
      301 cgtctggatt cccttcaagc gccagatcgc ggccgctgag acgggtttcc atttcgacga
      361 cttcgtcgcg cttgacgccg aggcgctggg cgacttcttc cgcctgtcgg ccggaaagag
      421 tgtcgacgcc ttcgtaatcg gccttcatgc tgcgcaggtt gaagaacagc ttgcgctggg
      481 ccttggtcgt cgccactttg accaggcgcc agttcttcag gatgtattcg tgaatctcgg
      541 ccttgatcca gtgcatcgcg aaggagacca ggcgaacgcc acggctcggg tcgaagcgct
      601 tgaccgcctt catcaggccg atgttgcctt cctggatcag gtcggcatgc ggcaatccgt
      661 agccgaggta gccgcgggcg atcgagatca ccagacgcag atgcgagagc accagttggc
      721 gcgcggcgtc gacatcgccg tcgttgtgga agcgctcggc cagccgggtt tcttccgctt
      781 cggacagcag ggggtaacga ttcgccgcct gaatgtaagc gtcgaggttg ccaacggccg
      841 aggggacggg aagcgccagg gcatgggtca tagcgtagaa tcctccttga tctgacagaa
      901 ctattttagc actcgctgcc tgagagtgct aagtatggag caagtttccg gcgcgtgtgt
      961 aacaaaattc aactgccatg ttcgtgactg gcgcgctgct gtatcgcggg cgcttcgcac
     1021 cgtcgccgac cggtccactg cacttcggtt cgctgatcgc cgcgctggcg agctgtctgg
     1081 aagcgcgctg ccggggtggt gagtggctgc tgcgcatcga ggacgtcgat accccgcgca
     1141 atgttcccgg tgccgccgac gcgatcctgc gtacgctgga ggctttcggt tttgtctggg
     1201 acggcccgtt gctctaccag agccgccgcg tggcggccta cgaggcggcg cttgacgtgc
     1261 tgcgcgggca gggcctgctc tatccctgcg cctgttcgcg cagcgagatc gccgcggcgg
     1321 ccagtcggac ggcggtcgat ggcggggcgg tctatcccgg cacctgtcgc gatgggttac
     1381 gccctggggc cgaggcgcgg gcctggcggc tgcgggtgga tgatgcagtg gtggtgttcg
     1441 aggaccgttt gcagggtatc gtcgaacagc gtctggccga gcaggtcggc gatttcgtcc
     1501 tgcgccgcgc cgacggcatt catgcttatc aactggcggt ggtcgtcgac gatgccagcc
     1561 agggcatcac cgacatcgtg cgcggcgccg acctgctcgc ttcgacgccg cgccagttgt
     1621 ggctgcagca gtgtctaggc tatccccgtc cgggttatgc ccatctgccg gtggcaacca
     1681 acccggccgg cgagaaatgg tcgaaacaga ccctggcgcc agcgctggcg gccgggaagg
     1741 cggctgccga actggtgcgg gcgctgcgct tcctcggcca accggcgccg gccgaactgg
     1801 cggcggcgcc ggtggccgag gtctgggact gggcgctggc caactggtcg ttcgccacaa
     1861 ttccccgggt gccggcgatc agtgttccgg cgactgcggc ttgaccggct cgccgccccg
     1921 ccagtgacgg tcgccgtaac cggcgatgcc gaccagcagg cggactaggc cgtagctgag
     1981 ccagcgcagg aaacgcgaat gccagggctg gccggcgatg tcctgtgcgc gtagttcgcg
     2041 gctgccggta tcgatcgcct catacaggct ggcacgcagc tcttcagcga aagcgcggtc
     2101 gcgggctacg aggttggctt ccttggcgag cagcaggctg aaggggtcga tgttggagga
     2161 gccgacggtg gcccagcggt ggtcgatgac cgcaaccttt gcgtggagga agctcttttc
     2221 gtattcgaag atgcggatgc cggcggccag cgcgctggcg tacagggtct gggtggcgta
     2281 gcgcaacagc ggatggtcgg tcttgccctg gagcaggatg tcgatccgta cgccgcgccg
     2341 cgccgccgcg taaagggcgc ggccgaaacg gatgccgggc aggaaatagg cgttggcgat
     2401 gaggatttcc tcttgcgccg cctcgatcgc gtcgatgtag gcgttgagga tgtcgttacg
     2461 gtggcggatg ttgtcgcgga tcaggaaggc ggcggtctgc tcgccggcgc tcgtgcagca
     2521 gggcgggagc agcggcgcca ggcggaagcg gcggcggaaa ttgacccagg tgacgatttc
     2581 ccacatgcgc cggacggcat ggtggatcgg cgccagcagc ggaccctcga cacgcaccgc
     2641 gtagtcgtag cgcggccgca tctcggctgg cgcattgtcg tcgtcgacga tgtttatgcc
     2701 gccgacgaaa gcgatcgtgc cgtcgatcac cgccagtttg cggtgcaggc ggcgcaggcg
     2761 atgccgttgc aagcggaagc ggcggatctc cggccggtag aagagggcgc ggacgccggc
     2821 ggcggcca
//
LOCUS       DAOSDX010000335         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12510_length_2826_cov_2.105377, whole genome shotgun sequence.
ACCESSION   DAOSDX010000335 DAOSDX010000000
VERSION     DAOSDX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12510_length_2826_cov_2.105377"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..703)
                     /gene="radC"
                     /locus_tag="PLN02_08130"
     CDS             complement(23..703)
                     /gene="radC"
                     /locus_tag="PLN02_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOI52330.1"
                     /translation="MAITDWPASERPRERLLALGPQALSDAELLAIYLRVGVRGRSAV
                     DLARELLRRHDGRLSKLATAPLAQLAATPGIGQAKAAQLQASFELARRALAEEMGERD
                     NFSSPELVRDWLRLQLAMHQHEVFMALWLDAQNRLLLAETLFTGTLTQTSVYPREVVK
                     RALSTNAAAVILAHNHPSGLAEPSSADQLLTGSLKQALALVDVRLLDHFIVAGNARPL
                     SFAERGLI"
     gene            749..1945
                     /gene="coaBC"
                     /locus_tag="PLN02_08135"
     CDS             749..1945
                     /gene="coaBC"
                     /locus_tag="PLN02_08135"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOI52331.1"
                     /translation="MTMELSGKRIVLGVTGGIAAYKAAELVRLMVRQGADVQVAMSEG
                     ATHFVTPTTFQALSGKPVFTDQWDPTMPNGMAHIDLSRQADLILVAPATADFLARIVH
                     GLADDLLATMVLARACPLLVAPAMNRQMWENPATQRNVAQLAVDGVGLLGPASGAQAC
                     GEVGLGRMLEPEEIVEQVIDFLAAKCLAGRRVLLTAGPTFEAIDPVRGITNLSSGRMG
                     YAIARAARQAGAEVTLVSGPVALVAPLGVERIMVRSALDMHGAVMARAAASDIFIGVA
                     AVADYRVANAAEHKLKKDLGGVPAIELVENPDILAEVAALPGGPFCVGFAAESRNLEA
                     YAQAKRQKKKIPLIAGNLIQDGFGGDDNRLVLFDDHGSHPLAPASKDVLARQLIEHIA
                     RLTGNL"
     gene            1945..2394
                     /gene="dut"
                     /locus_tag="PLN02_08140"
     CDS             1945..2394
                     /gene="dut"
                     /locus_tag="PLN02_08140"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOI52332.1"
                     /translation="MHRIDVKILDSRLKDNPPHYATPGSAGLDLRACLDEALELAPGQ
                     TVLVPTGLAIHLADPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSMWNRS
                     QLPFTIAPLERIAQLVVVPVLQVGFNVVDEFSASDRGEGGFGSTGHA"
     gene            2551..>2826
                     /locus_tag="PLN02_08145"
     CDS             2551..>2826
                     /locus_tag="PLN02_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOI52333.1"
                     /translation="MARAEEIVGGRCFLCHGMEGEAASAVFPRLAGQHWEYIAKQLGD
                     FKSGKRKSDTMKPQSEELTLEDMKALGRFFENKQVPGREVADADLVAV"
BASE COUNT          488 a          873 c          979 g          486 t
ORIGIN      
        1 gtcagggcaa gcttttttcg aatcaaatca gcccgcgttc ggcgaacgaa agcgggcggg
       61 cgttgccggc aacgatgaaa tggtcgagca ggcggacgtc gaccagcgcc agcgcctgct
      121 tgaggctgcc ggtcagcaac tggtcggcac tcgatggttc ggccagaccc gaaggatggt
      181 tgtgcgccag gatgacggcg gcggcattgg tgctcaaggc gcgcttgacg acttccctag
      241 gataaaccga ggtttgggtc agggtgccgg taaacaaggt ttccgcgagc agcagccggt
      301 tctgcgcgtc cagccagagg gccatgaaga cttcgtgctg gtgcatcgct aattgcaggc
      361 gcagccagtc gcggaccagc tccggcgagc tgaagttgtc gcgttcgccc atttcctcgg
      421 cgagggcgcg ccgcgccagt tcgaagctgg cctgcagttg cgccgccttg gcctggccga
      481 ttcccggcgt tgccgccagt tgcgccagcg gtgcggtggc cagcttgctc aggcggccgt
      541 cgtggcggcg caacagttcg cgggcgaggt cgacggcgct gcggccacgc acgccgacgc
      601 gcaggtagat cgccagcaat tcggcgtcgg acagtgcttg cggcccgagg gcgagcagtc
      661 tctcgcgtgg tctctcgctg gccggccaat cggtgatcgc catgttccgg actccagtag
      721 aatacgggtc cggctatctt accgataaat gacaatggaa ttaagtggca agcgtatcgt
      781 gctcggtgtc acgggcggca tcgccgccta caaggcagcg gaactggtcc gcctcatggt
      841 gcgccagggg gcagatgtcc aggtggcgat gagcgagggg gcgacccatt tcgtcacgcc
      901 gaccaccttc caggccctgt caggcaaacc ggtattcacc gaccagtggg acccgacgat
      961 gccgaacggg atggcgcata tcgatctttc gcggcaggcc gacctgattc tggtggcgcc
     1021 agcgacggcc gatttcctgg cgcgcatcgt tcatggcctg gccgacgatc tgctggcgac
     1081 catggtgctg gcgcgcgctt gcccgttgct ggtggcgccg gcgatgaacc gccagatgtg
     1141 ggagaacccg gcgacgcaac gcaacgtggc gcaactggcg gtggatggcg tcggcctgct
     1201 cggtccggcc agcggcgccc aggcctgcgg tgaggtcggg ctggggcgga tgctagagcc
     1261 cgaggaaatc gtcgagcagg tgatcgattt cctggccgcc aaatgcctgg ccgggcggcg
     1321 cgtgctgctc actgccgggc cgaccttcga ggcgatcgat ccggtgcgcg gcatcaccaa
     1381 cctgtcgtcc gggcgcatgg gctatgcaat cgcccgtgcc gcgcggcagg ccggtgccga
     1441 ggtcactctg gtttccggac cggtggcgct ggtggcgccg ctgggcgtcg agcggatcat
     1501 ggtacgcagc gcgctcgaca tgcacggcgc ggtgatggcg cgggcggcgg cttcggacat
     1561 cttcatcggc gttgccgcgg tcgccgatta ccgggtcgcc aatgccgccg agcacaagtt
     1621 gaagaaggat ctcggcggcg tgccggcgat cgagctggtc gagaatccgg atatcctggc
     1681 cgaagtcgcg gccttgccgg gcggcccgtt ctgcgtgggc tttgcggcgg aaagccgcaa
     1741 tctcgaggca tacgcgcaag ccaagcggca gaagaagaaa atcccgctga tcgccggcaa
     1801 cctgatccag gacggcttcg gcggcgacga caaccggctg gtgctgttcg acgaccacgg
     1861 cagccatccc ctcgcgccgg ccagcaagga cgtgctggcc cggcaactga tcgaacatat
     1921 tgcccgactg acaggaaatc tctaatgcat cgcatcgacg tcaagattct cgactcccgc
     1981 ctcaaggaca acccgccgca ttacgcgacg cccggttcag ctggtctgga cctgcgcgcc
     2041 tgcctcgacg aggcgcttga actggcgccg gggcagactg tgctggtgcc gaccgggctt
     2101 gccatccatc ttgccgatcc cgggctggcg gcgatgatcc tgccgcgctc gggcctcggc
     2161 cacaagcacg gcatcgttct cggcaacctg gtcggactga tcgacagcga ctatcagggg
     2221 caacttatgg tgtcgatgtg gaaccgtagt cagctaccat tcaccatcgc gccgctcgaa
     2281 cgcatcgccc agctcgtcgt ggtgccggtt ctgcaggtcg gtttcaacgt ggtcgatgaa
     2341 ttttccgcca gcgatcgcgg cgaaggcggc ttcggcagca ccgggcacgc ctgaacgcct
     2401 tgcaagtcct gctcaagcaa attacgattt gctgatgttc aacaacaaaa ccagaggcgg
     2461 ggacaaatga agggattggc aagatttgcg gcaggtttcg ctgtcgcggc tgggctggcc
     2521 ggcgcagcca gcgctcagga tgcggcggcg atggcgcgcg ccgaggaaat cgtcggcggg
     2581 cgttgcttcc tctgtcacgg catggaaggg gaagccgcca gcgcggtctt cccccggctg
     2641 gccgggcagc attgggagta catcgccaag cagctcggcg acttcaagtc gggcaagcgc
     2701 aagagcgaca cgatgaagcc gcagtccgaa gaactgacgc tggaagacat gaaggcgctg
     2761 gggcgtttct tcgagaacaa gcaggtgccc gggcgcgaag tcgccgatgc cgatctggtc
     2821 gccgtc
//
LOCUS       DAOSDX010000336         2816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12569_length_2816_cov_4.193770, whole genome shotgun sequence.
ACCESSION   DAOSDX010000336 DAOSDX010000000
VERSION     DAOSDX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12569_length_2816_cov_4.193770"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLN02_08150"
     CDS             complement(<1..143)
                     /locus_tag="PLN02_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOI52334.1"
                     /translation="MSDTLTMTAPTVPAPGLRATLREDLACVFERDPAARSTLEVLTT
                     YPGL"
     gene            complement(136..1272)
                     /gene="nifV"
                     /locus_tag="PLN02_08155"
     CDS             complement(136..1272)
                     /gene="nifV"
                     /locus_tag="PLN02_08155"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HOI52335.1"
                     /translation="MTQPFITINDTTLRDGEQSAGVAFSLDEKLAIARRLDAIGVPEL
                     EVGIPAMGEEERASIRAVAGLGLNAKLMVWSRMHPEDIAACRDLGAHLVDISVPASEQ
                     HLAYKLKRDRQWLLRELPKYIGAARDLGLDVGVGCEDASRADPDFLCALAEVAERSGA
                     RRLRFADTLGILEPFTTFDRIARLRANTGLEIEMHAHDDMGLATANTLAACKAGATHV
                     NTTVNGLGERAGNAPLEEVVLGLKKLHGIETGVELRGFPALSALVASASGRAVPWQKS
                     VVGAGAFTHEAGIHVDGLLKHPDNYQGFDPQEVGQAHRIVLGKHSGSRAVQVVYAGLG
                     VDLDEAQAQALLPQVRAFVASHKHPPETTDLIGLLASLRSPLHV"
     gene            complement(1456..2427)
                     /locus_tag="PLN02_08160"
     CDS             complement(1456..2427)
                     /locus_tag="PLN02_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52336.1"
                     /translation="MSKESTAWTEYCKSLSPAIAGACARTTVSAGPTALVKALDTALE
                     DWKFRHAFARGGWYRLGGVVDGSGERVSDNLETWLEQELAARDGDIDLLVDDYADQEL
                     YATRLVGQTHYLTACVGDAHEDFLLLEIEDLQEMRAHRLFAGEPGSLEELTDPRSGSE
                     KLQPLDLPFYTFRRLQHIGALLQRMLVQKPEAAPIHRMFEDWTKSSAGQTSAYSNHWV
                     IATREHLDRYQQPIIRAQPIATLAGEPPTFGAAATTRQLELHQALTQFDRGLGFPMAW
                     YFHMLTSKAVPHWVAQAVIEDALDGYAYLPPRDLEVVRHWLHAPYTL"
     gene            complement(2424..>2816)
                     /locus_tag="PLN02_08165"
     CDS             complement(2424..>2816)
                     /locus_tag="PLN02_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S) protein"
                     /protein_id="HOI52337.1"
                     /translation="EQLPQLIDDADYLIRATVARRLPHGQLSRLAKDPEREVRKVVAS
                     RLPPFALHLLADDPEPEVRALVAERALPGLAIALLGDPEWWVRLAAIDNVPPEQLAAL
                     AEDPEPEVRAAALARLSPTTAVRPEERT"
BASE COUNT          398 a          835 c         1082 g          501 t
ORIGIN      
        1 aggccgggat aggtggtcag cacctcgagc gtcgagcggg ccgccgggtc gcgttcgaaa
       61 acgcaggcga ggtcttcacg gagcgtggcg cgcaggcccg gtgcgggaac ggtgggggcg
      121 gtcatggtca gggtgtcaga catggagcgg gctcctgagg gaggcgagca ggccgatcag
      181 gtcggtcgtc tcgggcggat gcttgtggct ggcgacgaag gcgcgcacct gcggcagcag
      241 cgcctgcgcc tgggcttcgt cgaggtcgac gccgaggccg gcatagacca cctgcacggc
      301 gcgcgaaccg gaatgcttgc cgagcacgat gcggtgcgcc tggccgactt cctgcgggtc
      361 gaaaccctgg tagttgtccg ggtgcttgag caggccatcg acgtggatgc cggcttcgtg
      421 ggtgaaggcg ccagcgccga cgacgctctt ctgccagggc acggcgcgcc cggaagcgct
      481 ggcgacgagc gccgagagcg ccggaaaacc gcgcagttcg acgccggttt cgatgccatg
      541 caatttcttc agaccgagca cgacctcttc cagcggcgcg ttgccggcgc gttcgcccag
      601 cccgttgacc gtggtgttga cgtgcgtcgc gccggccttg caggcggcca gcgtgttggc
      661 ggtggccagc cccatgtcgt cgtgcgcgtg catctcgatt tcgaggccgg tattggcacg
      721 caggcgggcg atccggtcga aggtggtgaa tggctcaagg atgcccaggg tgtcggcgaa
      781 gcgcaggcgg cgggcgccgc tgcgttcggc gacttcggcc agcgcgcaga ggaagtcagg
      841 gtcggcgcgc gaggcgtcct cgcagccgac gccgacgtcg aggccgaggt cgcgggcggc
      901 gccgatgtac ttgggcagtt cgcgcagcag ccattggcgg tcgcgcttca acttgtaggc
      961 gaggtgctgt tcggaagccg gcaccgagat atcgaccagg tgggcgccga ggtcgcggca
     1021 ggcggcgatg tcctcggggt gcatgcggct ccacaccatc agcttcgcgt tgaggccgag
     1081 gccggcgacg gcgcggatcg acgcgcgctc ttcctcgccc atcgccggga tgccgacttc
     1141 cagttcggga acgccgatgg cgtcgaggcg tcgggcgatc gccagcttct cgtcgagcga
     1201 gaaggcaacc ccggcggatt gctcgccgtc gcgcagcgtg gtgtcgttga tggtgatgaa
     1261 gggctgggtc atggatggct ccttgctgct ttattagcaa gcccggtgcc agcttttata
     1321 ttgttgattt aattgagtta ttgtttttct ttgagtgaca ttgtcaatgt catgtcgtga
     1381 ttcctacacg gcgccggcgg tcaacgcgac gacgccccgg ccgggtcacg accggggcgt
     1441 cggtggcggg tggtcctata gcgtgtaggg cgcgtgcagc cagtggcgga ccacttccag
     1501 gtcgcgcggc ggcaggtagg catagccgtc gagtgcgtcc tcgatgaccg cctgcgccac
     1561 ccaatgcggg acggccttgc tggtcagcat gtggaaatac caggccatcg ggaagccgag
     1621 gccgcggtcg aactgagtca gcgcctggtg cagttcgagc tggcgggtcg tcgctgccgc
     1681 gccgaaagtc ggcggctcgc cggccagtgt ggcgatcggt tgggcacgga tgatcggttg
     1741 ctggtagcgg tcgaggtgtt cgcgggtggc gatcacccag tggttgctgt aggcgctggt
     1801 ctgcccggcg ctgctcttgg tccagtcctc gaacatgcga tggatcggcg ctgcttccgg
     1861 tttctggacc agcatgcgct gcagcagggc accgatgtgc tgcaggcggc ggaaagtgta
     1921 gaaaggcaga tcgagcggct gcagtttctc gctgccgctg cgtggatcgg tcaactcctc
     1981 gagcgagccc ggctcgccgg caaacaggcg gtgggcgcgc atttcctgga gatcctcgat
     2041 ttcgagcagg aggaagtctt cgtgggcgtc gccgacgcag gcggtgaggt aatgggtctg
     2101 gccgaccaga cgggtggcgt agagttcctg gtcggcgtaa tcgtcgacca ggaggtcgat
     2161 gtcgccgtcg cgggcggcga gttcctgttc cagccaggtc tcgagattgt cggaaacgcg
     2221 ttcgccgctg ccgtcgacga cgccgcccag gcggtaccag ccgccgcggg caaaggcgtg
     2281 gcggaatttc cagtcttcca gcgccgtgtc gagcgccttg accagcgccg tcgggccggc
     2341 gctgaccgtg gtcctggcgc aggcgccggc aatggccggc gacagcgact tgcagtattc
     2401 ggtccaggcg gtgctttcct tgctcatgtt ctttcctcgg ggcggacggc ggtggtgggg
     2461 ctgaggcggg cgagggcggc ggcccggact tcgggttccg ggtcctcggc cagcgcggcc
     2521 agttgttcgg gtgggacgtt gtcgatggcg gccaggcgca cccaccactc ggggtcgccg
     2581 agcaaggcga tggcgaggcc gggcaaggcg cgttcggcga ccagcgcgcg cacttccggt
     2641 tccgggtcgt cggccagcag gtgcagggcg aagggtggca ggcggctggc gacgaccttg
     2701 cgcacttcgc gttccgggtc cttggccagg cgcgagagct ggccgtgcgg caggcggcgg
     2761 gcgacggtgg cgcggatcag gtagtcggcg tcgtcgatca actgtggcaa ttgctc
//
LOCUS       DAOSDX010000337         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12651_length_2802_cov_2.863851, whole genome shotgun sequence.
ACCESSION   DAOSDX010000337 DAOSDX010000000
VERSION     DAOSDX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12651_length_2802_cov_2.863851"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLN02_08170"
     CDS             <1..738
                     /locus_tag="PLN02_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52338.1"
                     /translation="TLRAAQDELVQAAKLAVLGQMATGVTHELSQPLGAIRTLSANAA
                     EFMRRGDQATAERNLAIVEQLTEQMGNIIGPLKTLARKSPAVSSAVDVAQAVDSALFL
                     FESRLKKENVSVTRGIEAGRSIAWCDRNRLQQVLVNLIGNALDAMREEPVRRLSFSVD
                     VASSGMLALAVSDSGYGFSEEALAHLFEPFFTTKQPGEGLGLGLTISRDILRDFGGDL
                     MAGPAPGGGARFVVMLPPLPSLNRGTP"
     gene            735..2054
                     /locus_tag="PLN02_08175"
     CDS             735..2054
                     /locus_tag="PLN02_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOI52339.1"
                     /translation="MKTTLSVLLVEDDPHVRLGCEQAMQLAGIPVLAAASAEAAEMYL
                     SAEFPGIIVTDMRLPGASGMDLLRKVRGLDPDLPVIIITGHGDVTLAVEAMRNGAYDF
                     MQKPFSTDDLVDVVRRALEKRALVLEVEALRRRLDQRDDLEARLIGRSPQMQKVRRLI
                     LDVADAGVDVLIYGETGTGKEMVARCLHDLSRAGQENYVALNCGGMADNLLDSELFGH
                     EPGAFTGAQKRRIGKIEYASGGTLFLDEVESMPMSMQIKLLRVLQERVIERLGSNQQL
                     PVNCRVVAASKEDLKAWSEQGKFRADLYYRLNVVRIELPPLRERREDIPALYDAFLLQ
                     AAKRYNRPPPPLTTDTLHRLMAQDWPGNIRELKNAAECHVLGIGAAAGSGAPAAAPSL
                     TEAVENYERLLIADEFTRQGGNIQRTADALKTARTTLHDKLKKYGLL"
     gene            2088..2423
                     /locus_tag="PLN02_08180"
     CDS             2088..2423
                     /locus_tag="PLN02_08180"
                     /inference="COORDINATES: protein motif:HMM:NF020469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 family protein"
                     /protein_id="HOI52340.1"
                     /translation="MIVTFESSETGEVLMYAETAKTLLDIVGKATTARGVFTPPEMLP
                     AAAKLQAAVAEARRQAAALPEEEDEEDKSKPLPVSLPQRAWPLIDMLERTAKAGPQAH
                     IMWTAAADF"
     gene            complement(2427..>2802)
                     /locus_tag="PLN02_08185"
     CDS             complement(2427..>2802)
                     /locus_tag="PLN02_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52341.1"
                     /translation="ELALDTLPDTVGNGMVKGEIKENTRIVADKVHQAIIAFQFYDKL
                     VQRLAHVGLSLGELSDLVGEPARLYNPGEWVALQNRIKGKYSTREEVAMFDAVMNGVP
                     VQEAIERFMAEMKDKNDDIELF"
BASE COUNT          490 a          964 c          915 g          433 t
ORIGIN      
        1 acgctgcgcg ccgcccagga tgaactggtg caggcggcca agctcgccgt cctcggccag
       61 atggcgaccg gcgtcaccca tgaactgagc cagccgcttg gcgccatccg cacgctgtcg
      121 gccaacgccg ccgaattcat gcgccgcggc gaccaggcga cggccgagcg caacctcgcc
      181 atcgtcgagc agttgaccga gcagatgggc aacatcatcg ggccgctcaa aaccttggcc
      241 cgcaagtcgc cggcggtgtc gagcgcggtc gacgtggcgc aggcggtgga tagcgcgctg
      301 ttcctcttcg agtcgcggct gaagaaggag aacgtcagcg tcacgcgcgg catcgaagcc
      361 ggtcgcagca tcgcctggtg cgaccgcaac cgcctgcagc aggtactggt caacctgatc
      421 ggcaacgccc tcgacgcgat gcgcgaggag ccggtgcgcc gcttgagctt cagcgtcgat
      481 gtcgccagct cggggatgct ggcgctggcc gtcagcgaca gcggctacgg cttttccgaa
      541 gaggcgctgg cccacttgtt cgaacctttc ttcaccacca agcagccggg cgagggcctt
      601 ggtctggggc tgaccatttc gcgcgatata ctgcgcgact tcggcggcga cctgatggcc
      661 ggcccggcgc cgggcggcgg cgcccgcttc gtcgtcatgc tgccgccgct gccgtcgctc
      721 aaccggggaa cgccatgaag acgaccttgt ccgtcctgct cgtcgaagac gatccgcacg
      781 tccgcctggg ctgcgaacag gccatgcaac tggccggcat cccggtgctc gccgccgcct
      841 ccgccgaagc ggcggaaatg tacctgagcg ccgagttccc cggcatcatc gtcaccgaca
      901 tgcggctgcc cggcgccagc ggcatggacc tgctgcgcaa ggtgcgcggt ctcgaccccg
      961 acctgccggt gatcatcatc accggtcacg gcgacgtcac gctggccgtc gaagcgatgc
     1021 gcaacggcgc ctacgatttc atgcagaagc cgttttcgac cgacgatctg gtcgatgtcg
     1081 tgcgccgcgc gctggaaaag cgggcgctgg tgctggaagt cgaagcgctg cgccgccgcc
     1141 tcgaccagcg cgacgacctt gaagcccgcc tgatcggccg ctcgccgcag atgcagaagg
     1201 tgcgccgact gatcctcgac gtcgccgacg ccggcgtcga cgtgctgatc tacggcgaaa
     1261 ccggcaccgg caaggaaatg gtcgcccgct gcctgcacga cctgtcgcgc gccggccagg
     1321 agaactacgt cgcgctcaac tgcggcggca tggccgacaa cctgctcgac agcgaactct
     1381 tcggccacga gccgggtgcc ttcaccggcg cccagaagcg gcgcatcggc aagatcgagt
     1441 acgcgagcgg cggcacgctc ttcctcgacg aggtcgagag catgccgatg agcatgcaga
     1501 tcaagctgct gcgcgtgctg caggagcgcg tcatcgagcg tctcggttcg aaccagcaac
     1561 tgccggtcaa ttgccgcgtc gtcgcggcga gcaaggaaga cctcaaggcc tggtccgagc
     1621 agggcaagtt ccgcgccgac ctctactacc ggctcaacgt cgtgcgcatc gagctgccgc
     1681 cgctgcgcga gcggcgcgag gacatcccgg cgctctacga cgccttcctg ctgcaggccg
     1741 ccaaacgcta caaccggcca ccgccgccgc tcaccaccga caccctgcac cggctgatgg
     1801 cgcaggactg gccgggcaac atccgcgaac tgaagaacgc cgccgaatgc catgtcctcg
     1861 gcatcggcgc tgccgcgggc agtggcgcac cggccgccgc accgtcgttg accgaggcgg
     1921 tggagaacta cgagcgcctg ctcatcgccg acgaattcac gcgccagggc ggcaacatcc
     1981 agcgcaccgc cgacgcgctg aagacggcgc gcaccacatt gcacgacaag ctgaagaagt
     2041 acggtctgct atagtgcttc tccctccaga cgacacggac gaatgcgatg atcgtgacct
     2101 tcgaatcgag cgaaaccggc gaagtgctga tgtacgcaga aacggcgaag accctgctgg
     2161 acatcgtcgg caaggcgacc accgcgcgcg gcgtgttcac gccgccggaa atgctgccgg
     2221 cggcggcgaa actgcaggcg gcggtagccg aggcgcggcg tcaggctgcc gctttgccgg
     2281 aagaggaaga cgaggaagac aagagcaagc ctttgccggt cagcctgccg caacgcgcct
     2341 ggccgctgat cgacatgctt gagcgcaccg ccaaggccgg gccgcaggcc cacatcatgt
     2401 ggacggcggc ggccgatttc tgagcctcag aacagctcga tatcgtcgtt cttgtccttc
     2461 atttcggcca tgaagcgttc gatcgcttcc tggaccggga cgccgttcat taccgcgtcg
     2521 aacatggcga cttcctcgcg cgtcgagtac ttgcccttga tccggttctg cagcgccacc
     2581 cactcgcccg ggttgtagag acgggccggt tcgccgacca ggtcgctcag ttcgcccagg
     2641 ctgaggccga cgtgggcgag gcgctggacc agcttgtcgt agaactggaa ggcgatgatc
     2701 gcctgatgca ccttgtccgc gacgatgcgc gtgttttcct tgatctcgcc cttgaccatg
     2761 ccgttgccga cggtgtccgg cagggtatcg agggcgagct cg
//
LOCUS       DAOSDX010000338         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12672_length_2798_cov_3.823186, whole genome shotgun sequence.
ACCESSION   DAOSDX010000338 DAOSDX010000000
VERSION     DAOSDX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12672_length_2798_cov_3.823186"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PLN02_08190"
     CDS             complement(<1..1497)
                     /locus_tag="PLN02_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOI52342.1"
                     /translation="MPPYMTLRNHTYYFRQSVPPELRPLIGKREIKKSLGRNYPAAVS
                     LCKKLAVEADKAICDARAQFDSIPVDPFSSEGIRRTRPVPLTEVTPELETQFGNLIRN
                     ALLETDRNKRIAGMDAEEFAEYGRHIDQSIAALRKQLAMGNVTPVLEDSRILLIGRGY
                     NPNFSEEGWRKIAYTLTQAFLEAYEGIKKRQEGAVVTETSTDTLPSQFIVQNTRKTTP
                     DAGSEITWQMLYDVWEKECERRQSTKDAYLAAMKLFAEFSRKAPPQTTRDDALAFRDF
                     LRDVKSLSPGTIANKLGFIGTLFNAGRNTARLASHLPENPFADITVKRAKRGSGDNKR
                     LPFSDNDLKIIFSSPIYTQGERPEGGAGEACVWVPAIAYLTGMRLEEIATLTRSQFRT
                     DAKGNHYIHVLASKTDGGANRDVPIHPELIKAGLLNYVSLCTDRLFPQIQSTDESQSS
                     AFSKWFGRFLDNLQIKERSKVFHSFRHLFKDICRNARIEEAVIDQICGH"
     gene            complement(1598..1674)
                     /locus_tag="PLN02_08195"
     tRNA            complement(1598..1674)
                     /locus_tag="PLN02_08195"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1638..1640),aa:Asp,seq:gtc)
     gene            complement(1815..>2798)
                     /locus_tag="PLN02_08200"
     CDS             complement(1815..>2798)
                     /locus_tag="PLN02_08200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOI52343.1"
                     /translation="SFLLHRDDDARPTMLVLAPSAWLRFKAINRDGRWKVERSTTQPE
                     IRVEMRQGEVKNSLWDAVESGAISPSALLDLVQIFESEFDFTADTQPGDRFNILIETR
                     YADGVPVDFGRILAAQYVSGEQTLTGIGFATPGGDPERLSYYDAQGNSLKRMFLRSPL
                     EFSRISSGFTYRRPHPVLGGVRPHLAIDYAAPIGTPVWAVADGVVSFAGRNGGNGIQV
                     RVRHRAGYETYYNHLSRLGKGIRRGARVEQKQIIGLVGSTGISTGPHLDYRVKHGNDF
                     VNPLSEKFLPGKKLDAAQLPQFTQQARALLARLEQDNALQLTIDPSWQGKP"
BASE COUNT          486 a          759 c          916 g          637 t
ORIGIN      
        1 gtggccgcaa atctgatcga tcaccgcttc ttcgatccgg gcgttgcggc aaatgtcctt
       61 gaacaggtgc cggaagctgt ggaatacctt ggagcgttcc ttgatctgaa gattgtcgag
      121 gaaacggcca aaccattttg agaacgcaga gctctggctc tcgtcagtcg actggatctg
      181 cggaaacagt cgatccgtgc agagactgac gtagttcaag aggcctgcct tgatcagttc
      241 ggggtggatt ggcacgtcac gattggcgcc gccatcggtt ttcgatgcca gaacgtgaat
      301 gtagtgattg cccttggcgt cggttcggaa ttgcgagcgg gtcagggtgg cgatttcctc
      361 tagccgcatg ccggtcaggt aggcaatcgc cggcacccac acacaggctt cgccggcgcc
      421 gccttcgggg cgctcaccct gagtgtagat cgggctgctg aagatgattt tcaggtcgtt
      481 gtcgctgaaa ggcaggcgct tattgtcacc actgccgcgt ttggcccgtt tcaccgtgat
      541 atcggcaaag gggttttctg gtagatgact ggccaggcgt gcggtatttc gcccggcgtt
      601 gaacaacgtg ccgatgaaac cgagcttgtt ggctatcgtg ccgggggaga gggatttgac
      661 gtcccgcagg aaatcgcgga atgccagggc atcgtcgcgc gtggtttggg gcggggcctt
      721 gcggctgaat tcggcgaaca gcttcattgc tgccagatat gcatctttgg tgctctgtcg
      781 gcgctcgcac tctttttccc agacgtcgta cagcatctgc caggtgattt cgctgcctgc
      841 gtcgggtgtg gtcttgcggg tgttctggac gatgaactgg ctgggtaggg tgtctgtgct
      901 ggtttcagtc acgacagcgc cttcctggcg tttcttgatg ccttcgtagg cttccaggaa
      961 tgcctgggtc agggtgtagg cgatttttcg ccaaccttcc tcagagaagt tcgggttgta
     1021 accccggccg atcaagagga ttctcgaatc ctcgagcact ggcgtgacgt tgcccattgc
     1081 caattgtttg cgcagagcag cgatcgactg gtcaatgtgc cttccgtact cggcaaattc
     1141 ctcagcatcc atgcccgcaa tccgtttgtt ccggtcggtt tcgagcagcg cgttgcgaat
     1201 caggttgccg aactgggttt cgagttctgg ggtgacttca gtcaggggaa ccggtcgggt
     1261 acggcgaatt ccctcggagg agaagggatc gacagggatg ctgtcgaatt gggcgcgagc
     1321 atcacagatg gccttgtcgg cctcgacggc aagtttcttg caaaggctga cggctgccgg
     1381 gtaattgcga cccaaagact tcttgatttc ccgcttgccg atgagcgggc gcagctctgg
     1441 cgggacgctc tgccggaaat agtaggtatg gttcctgagg gtcatgtagg gtggcatgtc
     1501 cagtcccttc tgactgaact ttgtaccctg tgtttgtacc cttttgctcc ccatggtgac
     1561 tgattgtatc aatataatca gtcacttggg aataaattgg cggagtggac ggggctcgaa
     1621 cccgcgaccc ccggcgtgac aggccggtat tctaaccaac tgaactacca ctccgcgcta
     1681 aaccttggct tgctgaaagg ccggctatga aaccgggctt cgactgggat acgccgtgca
     1741 gcaaccgaag ccgcgcatta tagtgaactc cgacgggagg cgcaaggcgc tttttcgatt
     1801 taagcgtgtc gtgctcacgg tttcccttgc cagctagggt cgatggtcag ttgcagcgca
     1861 ttatcctgtt ccagtcgcgc gagcagggca cgggcctgct gggtgaattg cggaagctgt
     1921 gcggcgtcga gcttcttgcc ggggaggaat ttttcgctca ggggattgac gaagtcgttg
     1981 ccgtgtttca cccggtagtc gaggtgcggg ccggtggaga tgccggtcga gccgaccagg
     2041 ccgatgatct gtttctgctc gacgcgcgcg ccgcggcgga tgcccttgcc gaggcgcgac
     2101 aggtggttgt agtaggtttc gtagccggcg cggtggcgca ccctgacctg gatcccgttg
     2161 ccgccgttgc ggccggcgaa gctcaccacg ccatcggcaa ccgcccatac cggcgtgccg
     2221 atcggcgccg cgtaatcgat cgccaggtgc ggcctgacgc cgccgagcac cgggtgcggg
     2281 cgacggtagg taaagccgga gctgatgcgg gagaattcca gcggcgagcg caggaacatc
     2341 cttttcagcg aattgccttg cgcgtcgtaa tagctcagtc gttccgggtc gccgcctggg
     2401 gtggcaaagc cgatgccggt cagcgtctgt tcgccgctga cgtactgggc cgccaggatg
     2461 cgtccgaaat cgaccggcac gccatcggcg tagcgtgttt cgatcaggat gttgaagcgg
     2521 tcgccgggct gcgtgtcggc ggtgaagtcg aactcggatt cgaaaatctg caccaggtcg
     2581 agcaaggccg agggggagat ggcgccgctc tcgacagcgt cccagagcga attcttcact
     2641 tctccctggc gcatttcgac ccggatttcc ggttgggtcg tgctgcgttc gactttccag
     2701 cggccgtcgc ggttgatcgc cttgaagcgt agccaggcgc ttggtgcgag gaccagcatg
     2761 gtcggccggg catcgtcgtc gcgatgcaac aggaaaga
//
LOCUS       DAOSDX010000339         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12717_length_2790_cov_3.697623, whole genome shotgun sequence.
ACCESSION   DAOSDX010000339 DAOSDX010000000
VERSION     DAOSDX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12717_length_2790_cov_3.697623"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLN02_08205"
     CDS             <1..579
                     /locus_tag="PLN02_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52344.1"
                     /translation="IQDHAFTMNICRHTHGELSPRHFAPMLPAGMPRKILRRSLIFIC
                     LCVSACSEPSPAVYSIETLGKADRIAKVTQLFKTTQRTTLPSPIEDAQLAQFRIGDED
                     TALTIGPRDYETYLYVKLPVDQIDAWLRIFSRLDGPPLYTAPIKAYPPWMSRSDFSSL
                     EFYDADALLPGRHGWIAVSRQHGELFLFTHTM"
     gene            662..1216
                     /locus_tag="PLN02_08210"
     CDS             662..1216
                     /locus_tag="PLN02_08210"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOI52345.1"
                     /translation="MPTLLSHPAVPLALACALADAPVSRRLLVAGIAASILPDLDTLA
                     FRFGIAYGAQLGHRGFSHSLLCALLVALLGMLAANWLKASRQRCFAFLFLACASHGIL
                     DAFTNGGLGVALLWPYSEHRYFAPFRPIEVSPFDLQRFLARAPTLLASEIRWVWLPLT
                     GLAVLTLAGRWRFRARGKVAGALP"
     gene            complement(1496..2188)
                     /locus_tag="PLN02_08215"
     CDS             complement(1496..2188)
                     /locus_tag="PLN02_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOI52346.1"
                     /translation="MRPADRLFQIILLLRHGRVVTAAALAQALEVSERTIYRDIADLV
                     KSGAPIDGEAGVGYRLRRGYQVPPLMFAGDELEALAIGAKLVRAWGDSALGKAAEQAL
                     ARIDAVLPRHLEEKLEASSRRFLALDCLLPAALREPMAVLRAGMEGGRKVRVAYRRGD
                     GETSARTLWPLGLVFWGHCWTLGAWCELRDSFRTFRVDRIGAAELLEESFDDRYGHLW
                     ETYLQNARGEAK"
     gene            2338..2706
                     /locus_tag="PLN02_08220"
     CDS             2338..2706
                     /locus_tag="PLN02_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOI52347.1"
                     /translation="MQTPIAWFEIPTTDLERAIAFYENVFAVTLRRENCGGGEMAIFP
                     YECPAPGGALVAMPQLQPRDNGTLVYLHGGDDLAVPLARALAAGAKLIMAKTDLGNGV
                     GHIALFADSEGNTVGLYSQG"
BASE COUNT          485 a          982 c          825 g          498 t
ORIGIN      
        1 atccaggatc atgccttcac catgaatatt tgccggcaca cccacggcga gcttagcccg
       61 cgccacttcg cgccgatgct gccagcaggc atgccccgga agatactgcg ccgctcgctg
      121 atcttcatct gcctgtgcgt cagcgcatgc agcgaacctt cgccagcggt ctattcgatc
      181 gagacacttg gcaaggcaga tcgcattgcc aaggtgaccc aattgttcaa gacgacacaa
      241 cggaccacct tgccgtcacc catagaagat gcgcagctcg cccagttccg cattggtgat
      301 gaggacaccg ccctgacgat cggcccgcgc gactacgaga cctacctcta tgtgaagctt
      361 cccgtcgatc agatcgacgc ctggctaagg atcttcagtc ggctcgatgg cccacccctc
      421 tatacggcgc cgataaaggc ctatcccccc tggatgagcc gaagcgactt ctcgtcactg
      481 gagttttatg atgccgatgc cttgctgcca ggccgtcatg gctggatcgc ggtttcgcgc
      541 cagcatggag aacttttctt gtttacgcac accatgtgag cacaggcaga ccgaaaatat
      601 tggcaacggc atatactgcc cccccgtacc gccagcggac catcccacca acccgtatcc
      661 agtgcccacc ctgctcagcc acccggcagt cccgcttgcg ctggcctgcg ccctggccga
      721 tgcgccggtt tcccggcgcc tgctggtcgc cgggatcgcg gcatccatcc tgcccgatct
      781 cgataccctg gccttccgtt tcggcattgc ctacggcgcc cagttgggac atcgcggatt
      841 cagccactcc ctgctctgcg cgctgctcgt ggcgctgctc ggcatgctcg ccgccaactg
      901 gctgaaggcg agtcgccagc gctgcttcgc ctttctcttc ctggcctgcg cctcgcacgg
      961 catccttgat gcattcacca atggcggact gggcgttgcc ttgctatggc catattcgga
     1021 acaccgctat ttcgccccgt tccgcccgat cgaagtatcg ccgttcgacc tgcaacgctt
     1081 ccttgcccgc gcgccgacgc tcctcgcctc ggaaatccgc tgggtctggc tgccgctgac
     1141 cggcctggcc gtgttgacac tggccggccg ctggcggttt cgagcccgtg gcaaagtggc
     1201 tggcgccctc ccctgaccag acaccagcta cttcccgagc gccacccgcg ttgcacagga
     1261 ggtggccgga ggcatgctct ccgcgccctc aaaacgatat tccgcacctc ccgcaagcga
     1321 taagcgaaca gagcatcgcc cccgaaggct atacggtgat cagcacggag gtctggtggc
     1381 agattactgt gaccccgccc tagggcatgt ggcttcgaca aagcgccttc agctacacaa
     1441 gcgctacagg cggcagcatg caacccggaa ggacagcccc cgccttgccg gggctctact
     1501 tcgcctcgcc cctggcgttc tgcagatacg tctcccacag gtgcccgtag cgatcgtcga
     1561 agctttcttc gagcagttcg gcggcgccga tccggtcgac gcggaaggta cggaagctgt
     1621 cgcgcagttc gcaccaggcg ccgagggtcc agcagtggcc ccagaagacg aggccgagcg
     1681 gccagagcgt gcgtgcggag gtttcgccgt cgccgcgccg gtaggcaacg cggactttgc
     1741 gcccgccctc catgccggcg cgcaagaccg ccatcggttc gcgcagcgcc gccggcaaca
     1801 ggcaatcgag ggcgagaaaa cggcggctgc tggcttccag tttttcttcc agatggcgcg
     1861 gcaataccgc gtcgatgcgc gccagcgcct gttccgccgc cttgcccagc gcgctgtcgc
     1921 cccaggcgcg gaccagcttg gcgccaatcg ccagggcttc cagttcgtcg ccggcaaaca
     1981 tcagcggcgg cacctggtaa ccccggcgca gacggtagcc gacgccggct tcgccgtcga
     2041 tcggggcgcc gctcttcacc aggtcggcaa tgtcgcgata gatcgtgcgc tcggacactt
     2101 ccagcgcctg ggccaaggcg gcggcggtga cgacgcggcc gtggcgcagc agcaagatga
     2161 tctggaagag gcgatcggcg gggcgcatgg ggctagtccg aaacgatgga atttcaggat
     2221 agcacgcgct ttttcccggc caggccaagc ctcctgacac cctgttgtca ggagcccggt
     2281 tcgagaatgg cggccagcaa cgacagcccg tcgctgcatt tcagcggaga aaacctcatg
     2341 caaaccccca tcgcctggtt cgaaatcccc acgaccgatc tggaacgcgc catcgccttc
     2401 tacgaaaacg tcttcgccgt caccctgcgt cgcgagaatt gcggcggcgg cgagatggcc
     2461 atcttcccct acgaatgccc ggcgcccggc ggcgcgctgg tggcgatgcc gcagttgcag
     2521 ccgcgcgaca acggcacgct ggtctatctc cacggcggcg acgacctcgc cgtaccgctc
     2581 gcccgtgcgc ttgcagcggg cgccaagctc atcatggcga agaccgacct cggcaacggc
     2641 gtcggccata tcgcgctgtt cgccgacagc gaaggcaata cggtcggcct gtactctcag
     2701 ggctgaggcg aacggcctgg cagggcacga caaaattgaa acggcgaggt acatcgcctc
     2761 gccgtgttat tgctcctccg ccctgttcgg
//
LOCUS       DAOSDX010000340         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12741_length_2785_cov_3.727839, whole genome shotgun sequence.
ACCESSION   DAOSDX010000340 DAOSDX010000000
VERSION     DAOSDX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12741_length_2785_cov_3.727839"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLN02_08225"
     CDS             <1..562
                     /locus_tag="PLN02_08225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HOI52348.1"
                     /translation="LAPPQPDQPLVAVPGAGTEVPIWLLGSSLFSARLAAERGLPYAF
                     ASHFAPALLHQALAVYRGNFRPSATLAKPYVIVGVPLVAAETDEEADFLATSIFQRVL
                     GILRGDRRPLPPPVAGFLAGLSMQEKAGIGDFLGAAVIGGPDRVREGLSALRQATQAD
                     ELMLVCDIFDPALRLRALDIAAAACA"
     gene            620..1774
                     /locus_tag="PLN02_08230"
     CDS             620..1774
                     /locus_tag="PLN02_08230"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOI52349.1"
                     /translation="MNHLFEFNYHNPTRIVFGPDSFAQLPSLLPAEARVLLLYGGGSI
                     KQNGVYDGIRAALAGRDVVEFGGVEANPTLVTLNRAVDLVRREKIGFILGVGGGSVAD
                     GAKYVACAALYDGDGWDIVIGKHQPQQALPVGVVLTLPATGSESNAGSVVTNTATQDK
                     RAFFVPPARPRFAVLNPTVMASLPVRQLENGIVDAFVHVCEQYLTYPVGALVQDGYAE
                     AVMKALVKLGATFDRHTEPEWQQNLMWAANQALCGIIGVGVPQDWATHRIAVELTALW
                     GIDHGRTLAIIQPALLRELIEYKRDKLEQFGREVFGLDAAGAEDAIAAMEAFYRSVNM
                     PLHLRDAGISEADAADRVLAGLRAHGKMALGGHAGIDEAKTERIVRAACA"
     gene            complement(1800..2036)
                     /locus_tag="PLN02_08235"
     CDS             complement(1800..2036)
                     /locus_tag="PLN02_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HOI52350.1"
                     /translation="MSQLALRLPDSLHERARLLAQRDNTSLNQFIALAVAEKVSALET
                     ASFFSERAARGNLDELRAILDKVPDVAPQAGDER"
     gene            complement(2033..2473)
                     /locus_tag="PLN02_08240"
     CDS             complement(2033..2473)
                     /locus_tag="PLN02_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOI52351.1"
                     /translation="MGNIMRVVLDTNILVAGLSSRRGASHELLRLALAQRLRLLAAPA
                     LWLEYESVLKRQEIQRMHGLSGIEIDRFLDALAILVEPVSMNYLWRPQLRDPKDEMVL
                     ETALNACADALVTFNQRDFQPAADNFRLRLLLPAACLLLMEETP"
     gene            complement(2521..>2785)
                     /locus_tag="PLN02_08245"
     CDS             complement(2521..>2785)
                     /locus_tag="PLN02_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOI52352.1"
                     /translation="AYVLKLEDYLLATWHASTRPSELDLTADDSKWLGLEVRAHQLQD
                     ETHATVDFVARYRIAGRGHRLHELSRFVREDGRWYYVDGEFLA"
BASE COUNT          471 a          946 c          916 g          452 t
ORIGIN      
        1 gctcgccccg ccccagcccg accagccgct ggtcgccgtg cccggcgccg gcaccgaggt
       61 gccgatctgg ctgctcggtt ccagcctgtt ctcggcccgc ctcgccgccg agcgcggcct
      121 gccctacgcc tttgcgtcgc acttcgcgcc ggccctgctg catcaggcgc tggccgtcta
      181 tcgcggcaac ttccggccat cggctacgct ggccaagccc tacgtcatcg tcggcgtgcc
      241 gctggtcgcc gccgaaaccg acgaggaagc cgacttcctc gccaccagca tcttccagcg
      301 cgtgctcggc atcctgcgcg gcgaccgccg gccgttgccg ccgcccgtgg caggcttcct
      361 cgccgggctg tcgatgcagg agaaagccgg catcggcgat ttcctcggcg ccgccgtgat
      421 cggcgggccg gaccgcgtgc gcgaaggact gagcgcgctg cgccaggcga cgcaggccga
      481 cgagctgatg ctggtctgcg acatcttcga cccggcgctg cgcctgcgcg cgctcgacat
      541 cgccgccgcc gcttgcgcct gagatttttc cgcctatcct gcggtcgacc gcaaaactga
      601 agccaaaacg gagacagcca tgaaccatct gttcgaattc aactatcaca acccgacgcg
      661 catcgtcttc ggccccgaca gcttcgcgca actgccttcg ctgctgccag ccgaggcgcg
      721 cgtgctgctc ctctacggcg gcggctcgat caagcagaac ggcgtctacg acggcatccg
      781 cgccgcgctc gccgggcgcg acgtggtcga gttcggcggc gtcgaggcca acccgacgct
      841 ggtgacgctg aatcgggcgg tcgacctggt ccgacgcgaa aaaatcggct tcatcctcgg
      901 cgtcggcggc ggttcggtcg ccgacggcgc caagtacgtc gcctgcgccg cgctttacga
      961 cggcgacggc tgggacatcg tcatcggcaa gcaccagccg cagcaggcgc tgccggtcgg
     1021 cgtcgtgctg acgctgccgg ccaccggttc ggaatcgaac gccggctcgg tggtcaccaa
     1081 caccgcaacc caggacaagc gcgccttctt cgtgccgccg gcacggccgc gcttcgccgt
     1141 gctcaacccg acggtgatgg ccagcctgcc ggtgcgccag ctggaaaacg gcatcgtcga
     1201 tgccttcgtc catgtctgcg aacaatacct gacctacccg gtcggcgcgc tggtccagga
     1261 cggttacgcc gaggcggtga tgaaggcgct ggtcaagctg ggcgcgacct tcgaccggca
     1321 caccgagccc gaatggcaac agaacctgat gtgggcggcc aaccaggcgc tgtgcggcat
     1381 catcggcgtc ggcgtgccgc aggactgggc gacgcaccgc atcgccgtcg aactgactgc
     1441 gctgtggggc atcgaccacg gccgcacgct ggcgatcatc cagccggcgc tgctgcgcga
     1501 gctgatcgag tacaagcggg acaagctgga acagttcggc cgcgaggtct tcggcctcga
     1561 cgcggcgggc gccgaagacg ccatcgccgc catggaagcc ttctaccgca gcgtcaacat
     1621 gccgctccac ctgcgcgacg ccggcatcag cgaagccgac gccgccgacc gggtgctcgc
     1681 cggcctgcgc gcccacggca agatggcgct cggcggccac gccgggatcg acgaagcgaa
     1741 gacggaaagg atcgtgcgcg cggcttgcgc ttgagactgc gccgggtgac gggacggatt
     1801 cagcgttcgt cgccggcctg cggcgctacg tcaggcactt tgtcgagaat cgcgcgcagc
     1861 tcgtccaggt ttccccgggc tgcgcgttcg ctgaagaaac tggcggtttc cagcgcggaa
     1921 actttctccg caaccgccag cgcgatgaac tggttcagtg aggtgttgtc gcgctgagcg
     1981 agcaggcgag cgcgttcgtg cagggaatcg ggcaggcgca gggcaagttg gctcatggtg
     2041 tttcctccat caggagcagg catgcggccg gcaacagcag gcgcaggcgg aaattgtcag
     2101 cagccggctg aaaatcgcgc tggttgaaag taaccagcgc atcagcgcag gcattcaggg
     2161 cggtctccag gaccatttca tccttggggt cgcgtaattg cggccgccaa aggtaattca
     2221 tactgaccgg ttcgaccaga atggcgagcg catccagaaa gcgatcgatt tcgatcccgg
     2281 acagaccatg catgcgctgg atttcctggc gtttgagcac gctctcgtat tccagccata
     2341 gtgctggcgc cgccaaaagc cgcaaacgtt gcgcgagggc aaggcgcaac aactcgtgcg
     2401 acgcaccacg gcggctgctc agacctgcaa caaggatgtt ggtatccagg acgactcgca
     2461 tgatattgcc catgattcta ctggcagtat caaatgtcaa ggcaagccga aagaatgccc
     2521 ttaggccaga aactcgccgt ccacgtagta ccagcgcccg tcctcgcgga cgaaacggga
     2581 gagttcgtgc agccggtggc cgcgcccggc gatgcggtag cgggcgacga agtcgaccgt
     2641 cgcgtgcgtc tcgtcctgaa gttggtgagc gcgtacttcc aggcccagcc atttgctgtc
     2701 gtcggcggtg aggtcgagtt cgctcggccg cgtcgacgcg tgccaggtcg ccagcaggta
     2761 gtcttcgagc ttgaggacgt aggcg
//
LOCUS       DAOSDX010000341         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12743_length_2785_cov_2.985348, whole genome shotgun sequence.
ACCESSION   DAOSDX010000341 DAOSDX010000000
VERSION     DAOSDX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12743_length_2785_cov_2.985348"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..822
                     /locus_tag="PLN02_08250"
     CDS             55..822
                     /locus_tag="PLN02_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52353.1"
                     /translation="MFRKFRILILLLALATVALAAWRAENRLTAWERTVHVALYPLAA
                     DNSPQTADYLKTLDNDSFAEIGQWIDQEMQRHGKRVLQPVVVRVAPAQQALPPLPAAG
                     GSALDNLLWSLKLRWWAAKHDDIAGPQPQIRLFVLYHDPDSNERLDHSVGLSKGQLGL
                     IHAFASRRQQRQNAVVIAHEMLHTFGATDKYDLRSLQPIFPDGYAEPERQPRLPQRLA
                     EIMGGRIPQAPDRAEMPASLRQAVIGPLTAREIGLTP"
     gene            877..>2785
                     /locus_tag="PLN02_08255"
     CDS             877..>2785
                     /locus_tag="PLN02_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOI52354.1"
                     /translation="MSAAPSLIQLPALPRPGQRVDLEALAGSADALALAELAAANPGR
                     TLAVVTASAADAQRLLEEIPWFAPQLKVRLLPDWETLPYDHFSPHQDLVSERLATLWA
                     ALQGEVQILLVPAATAVNRLAPPEFMAAYTFEFKKGQTLDAEKFRAQVTLAGYANVTQ
                     VVSPGEYSIRGGLIDLFPMGSQLPYRLDLFDDEVESIKTFDVDTQRTVYPVPEVRLLP
                     AREFPLDDKGRTHFRQAFRERFEGDPAKSGIYKDISTGIASAGIEYYLPLFFDATATL
                     FDYLPKDAIFVTHGDAPAAIAAFWNDTRSRYNLLQGDKARPLLAPEELFLTDEAFFTA
                     AKAYGRLALDAKNTATGKLPNVAVDRRSDDPLGALKNHLASYPGRVLLLAETAGRRET
                     LAAMFAEHGLRPAACADFAAFASGSERLALTVSPLQQGFALDRVAFVSETELFAGSPR
                     RTRREAVRKASFDNWLKDLTELKVGDPVVHESHGIGRYRGLVRMDLGLGEQEFLELHY
                     ANDAKLFVPVAQLHVISRYSGAEPDAAPLHTLGSGQWEKAKRKAAEQARDTAAELLAL
                     YAARAARQGHAFDFSENDYEAFADGFGFEETADQAQAIAAVIADMKSGKPMDRLVCGD
                     VGFGKTEVALRA"
BASE COUNT          483 a         1058 c          834 g          410 t
ORIGIN      
        1 gatagcgtga tcatataatg tagccctcaa cttgcgcatc ccctttaacg tcccatgttc
       61 cgcaaattcc gcatcctgat cctgctcctc gccctggcca ccgttgccct cgccgcctgg
      121 cgcgcggaga accggctgac cgcctgggag cgcaccgtcc atgtcgcgct ttacccgctc
      181 gccgccgaca attcgccgca gaccgcggac tacctgaaga ccctggacaa cgacagcttc
      241 gcagaaatcg gccagtggat cgatcaggaa atgcaacgcc acggcaaacg cgtgctgcaa
      301 ccggtcgtcg tccgcgtcgc cccggcgcag caggccttgc cgccgctacc ggcggccggc
      361 ggcagcgccc tcgacaacct gctgtggagc ctcaagctgc gctggtgggc ggcaaaacac
      421 gacgacattg ccgggccaca gccgcagatt cgcctgttcg tcctctatca cgacccggac
      481 agcaacgaac ggctcgatca ctcggtcggc ctcagcaagg gccagctcgg actgatccac
      541 gcctttgcca gccgccgcca gcagcgccag aacgccgtcg tcatcgccca cgaaatgctg
      601 cataccttcg gcgccaccga caagtacgac ctgcgcagcc tgcaaccgat cttccccgac
      661 ggctacgccg aaccggagcg acagccccgc ctgccgcagc gactggccga aatcatgggc
      721 ggccgcattc cgcaggcgcc ggaccgggcc gagatgccgg ccagcctgcg ccaagcggtg
      781 atcggcccgc tcaccgcgcg cgagattggc ctgacgccgt agggggcggg cgcgccaacg
      841 gctataatcc cagccccgtc gtttgaccct gccgccgtgt ccgctgcccc ttccctgatt
      901 caacttcccg ccctgcccag acccggacag cgtgtcgatc tcgaagcgct cgccggctcg
      961 gccgatgcgc tggcgctagc cgaactggcc gccgccaatc ccgggcggac gctggccgtg
     1021 gtcaccgcca gcgccgccga cgcgcagcgt ctgctggaag aaattccctg gttcgcgccg
     1081 caactcaagg tgcgcctgct gccggactgg gaaaccctgc cctacgacca tttctcgcca
     1141 caccaggacc tcgtctccga acgcctggca acgctgtggg cggcattgca gggcgaagtg
     1201 cagatcctgc tggtgccggc ggcaaccgcg gtcaaccggc tggcgccgcc ggaattcatg
     1261 gccgcctaca cgtttgaatt caagaagggc cagacgctcg acgccgagaa attccgcgcc
     1321 caggtcacgc tggccggcta cgccaacgtc acccaggtgg tgtcgcccgg cgaatactcg
     1381 atccgcggcg gcctgatcga cctcttcccg atgggctcgc aactgcccta ccggctcgac
     1441 ctgttcgacg acgaggtcga gagcatcaag accttcgacg tcgacaccca gcggaccgtc
     1501 tatccggtgc cggaagtccg cctgctgccg gcgcgcgaat ttccgctcga cgacaagggc
     1561 cgcacccact tccgccaggc cttccgcgag cgtttcgaag gcgacccggc caagtccggc
     1621 atctacaagg acatctcgac cggcatcgcc agcgccggca tcgagtacta cctgccgctg
     1681 ttcttcgacg cgacggcgac gctgttcgac tacctgccca aggacgcgat cttcgtcacc
     1741 cacggcgacg cgccggccgc catcgctgcc ttctggaacg acacccgctc gcgctacaac
     1801 ctgctgcagg gcgacaaggc gcggccgctg ctggcgccgg aagaactgtt cctcaccgac
     1861 gaagccttct tcaccgccgc caaggcatac ggccggctgg cgctcgacgc gaagaacacc
     1921 gccaccggca agctgcccaa cgtcgccgtc gaccgccgca gcgacgatcc gctcggcgca
     1981 ttgaagaacc atctcgccag ctatcccggg cgcgtgctgc tgctcgccga gaccgccggc
     2041 cgccgcgaga cgctggccgc gatgttcgcc gaacacggtc tcaggcccgc cgcctgcgcc
     2101 gactttgccg ccttcgccag cggcagcgaa cgcctggcgc tgaccgtcag cccgctgcag
     2161 cagggctttg ccctcgaccg ggtcgccttc gtcagcgaaa ccgagctgtt cgccggcagc
     2221 ccgcgccgca cccggcgcga agcggtgcgc aaggcgagct tcgacaactg gctgaaggac
     2281 ctcaccgaac tcaaggtcgg cgacccggtg gtgcatgaaa gccacggcat cggccgctat
     2341 cgcggcctgg tacggatgga cctcggcctc ggcgagcagg aattcctcga actgcactac
     2401 gccaacgatg ccaagctctt cgtgccggtg gcgcaactgc acgtgatctc ccgttattcc
     2461 ggcgccgagc cggacgccgc gccgcttcac acgctcggtt ccggccagtg ggaaaaggcc
     2521 aagcgcaaag ccgccgaaca ggcgcgcgac accgccgccg aactgctcgc gctctacgcc
     2581 gcgcgcgccg cccgtcaggg ccacgccttc gacttctcgg aaaacgacta cgaggccttc
     2641 gccgacggct tcggtttcga ggaaaccgcc gaccaggcgc aggccatcgc cgccgtcatc
     2701 gccgacatga agtcgggcaa gccgatggac cgcctggtct gcggcgacgt cggcttcggc
     2761 aagaccgaag tcgcactgcg cgccg
//
LOCUS       DAOSDX010000342         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12813_length_2773_cov_2.969463, whole genome shotgun sequence.
ACCESSION   DAOSDX010000342 DAOSDX010000000
VERSION     DAOSDX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12813_length_2773_cov_2.969463"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="soxA"
                     /locus_tag="PLN02_08260"
     CDS             <1..680
                     /gene="soxA"
                     /locus_tag="PLN02_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HOI52355.1"
                     /translation="SELVAAEGEELWKTARGPKNATLERCDLGLGPGVIKGASAQLPR
                     YFKDTGKVQDLETRLMTCMETLQGIDPQEVVKAKWLKGEREKMAALVAYVVTQSNGHK
                     IKVDMRPPAMKQMYEMGKMAFYYRAGPMDFSCATCHGDQGKRIRMQELPDLRTNAGAA
                     AGWGSWPAYRVSNGQFWTMQHRLNDCFRQQRTAEPIYGSDVTIALSVYLAANANGGKV
                     VTPGIKR"
     gene            691..1296
                     /gene="soxX"
                     /locus_tag="PLN02_08265"
     CDS             691..1296
                     /gene="soxX"
                     /locus_tag="PLN02_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HOI52356.1"
                     /translation="MKKLTTLALLLAALPTAWAGSEDTYAEFKAMMDRSFKTTGIAAK
                     HRMYQLDLQKACSQDKPPAKAEQLKIEAEQLKTIRYPADGKYFGDWKAGEKIAVDGRG
                     LTWTDKTPYNNGGGCYNCHQMSHHEVSYGTIGPSLLEYGKTRGNSEDVVRYTWGKIYN
                     AKAFNACSNMPRNGDAEILTEKQMKDLMAYLFDPQSPVNKK"
     gene            1353..>2773
                     /gene="soxB"
                     /locus_tag="PLN02_08270"
     CDS             1353..>2773
                     /gene="soxB"
                     /locus_tag="PLN02_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HOI52357.1"
                     /translation="MSMNRRELLQVLAVAAAGGMSLHSELALAEKGAGKLYDLPKFGN
                     VSLLHITDCHAQLNPIYFREPSVNLGFGDQFGKVPHLVGDKLLKHFGFKPNSIEAHAY
                     TYLNFEKAAETYGKVGGFAHLATLVKRMKARNPGALLLDGGDTWQGSGTALWTNAQDM
                     VDACKALGVNVMTLHWEATYGEARVKEIEEKDFAGVVDIVAQNVKTADFGDPVFKPYV
                     IKNMNGVPVAIVGQAFPYTPIANPRWQVPNWSYGIQEENMQAVVDEARAKGAQVVVVL
                     SHNGMDVDLKMASRVRGIDAIMGGHTHDGMPAPVIVKNAGGQTLVTNAGSNGKYLGVL
                     DFDVKNGKIAGYRYKLLPVFAKLLPADPAMQALIDKVRAPYLGKLNEQLAISEGTLYR
                     RGNFNGTFDQVIVDALIKEKDAEIAFSPGFRWGTSLLPGQPILMEHVLDQTAITYPWT
                     TVSNMSGEMIKTVRGFGYKLEITP"
BASE COUNT          591 a          893 c          849 g          440 t
ORIGIN      
        1 cttccgaact ggtcgccgcc gaaggcgagg agttgtggaa gaccgcgcgc ggaccgaaga
       61 acgcgaccct ggaacgctgc gacctcgggc tcggccccgg cgtcatcaag ggcgccagcg
      121 cccagctgcc gcgctatttc aaggataccg gcaaggtaca ggacctcgag acgcgtctga
      181 tgacctgcat ggaaaccctg caggggatcg atcctcagga agtcgtcaag gccaaatggc
      241 tgaagggcga acgcgagaag atggcggccc tggtcgccta tgtcgtgacc cagtcgaacg
      301 gccacaagat caaggtcgac atgcgtccgc ccgccatgaa gcagatgtac gagatgggca
      361 agatggcctt ctactaccgc gccgggccga tggatttctc ctgcgccacc tgtcacggtg
      421 accagggcaa gcgcatccgc atgcaggaac tgccggattt gcgcaccaat gccggcgctg
      481 ccgccggctg ggggagctgg ccggcctacc gggtgtcgaa cggtcagttc tggaccatgc
      541 agcatcgtct caacgattgc ttccggcagc agcgtaccgc cgagccgatc tacggttccg
      601 acgtgaccat cgccctctcc gtctatctgg cggccaacgc caatggcggc aaggtagtga
      661 cccccggcat caagcgctaa ggagacgaag atgaagaaac tcaccacact cgccctcctg
      721 ctggctgcct tgccgacggc ctgggccggc agcgaggata cctacgccga gttcaaggcg
      781 atgatggatc gttcgttcaa gaccaccggt atcgccgcca agcaccggat gtatcagctc
      841 gatctgcaga aggcctgctc gcaggacaag ccgccggcca aggcggagca gttgaagatc
      901 gaggcggaac agttgaagac catccgctac ccggccgacg gcaaatactt cggcgattgg
      961 aaggccggcg aaaagattgc cgtcgacggc cgcggcctga cctggaccga caagacgccg
     1021 tacaacaacg gcggtggctg ctacaactgt caccagatga gccaccacga agtctcctac
     1081 ggcaccatcg gtcccagtct gctcgaatac ggcaagacgc gcggcaacag cgaggatgtc
     1141 gtcagataca cctggggcaa gatctacaac gccaaggcct tcaacgcctg cagcaacatg
     1201 ccgcgcaatg gcgatgccga aatcctgacc gaaaaacaga tgaaggatct gatggcctat
     1261 ctgttcgatc cccagtcgcc ggtcaacaag aagtaacacc gatgtgacca gcccggcgga
     1321 ttcccgccgg gcttttcttc agggaagcat tcatgagcat gaatcgtcga gaattgctcc
     1381 aggtgctggc ggtggctgcc gctggcggca tgtccttgca cagcgaactg gcgctggccg
     1441 aaaagggggc cggcaagctg tacgacctgc ctaagttcgg caacgtcagc ctgctgcaca
     1501 tcaccgactg ccacgcccag ctcaatccga tctattttcg tgagccgagc gtcaatctcg
     1561 gtttcggcga ccagttcggc aaggtgccgc acctggtcgg cgacaagctg ctcaagcact
     1621 tcgggttcaa gccgaacagc atcgaggcgc atgcctacac ctatctgaat ttcgagaagg
     1681 ccgccgagac ctacggcaag gtcggcggct tcgcccacct ggcgacgctt gtcaagcgca
     1741 tgaaggcgcg caatccgggg gcgctgctgc tcgacggcgg cgacacctgg cagggttccg
     1801 gcaccgcgct gtggaccaac gcccaggaca tggtcgacgc ctgcaaggcg ctcggcgtca
     1861 atgtcatgac cctgcactgg gaagccacct atggcgaggc gcgggtcaag gaaatcgagg
     1921 agaaggactt cgccggcgtc gtcgacatcg tcgcccagaa cgtcaagacc gccgatttcg
     1981 gcgatccggt gttcaagccc tacgtgatca agaacatgaa cggcgtgccg gtcgccatcg
     2041 tcggccaggc cttcccctac acgccgatcg ccaacccgcg ctggcaggtg ccgaactgga
     2101 gctacggcat ccaggaagag aacatgcagg cggtggtcga cgaagcccgc gccaagggcg
     2161 cccaggtcgt cgtcgtcttg tcgcacaacg gcatggacgt cgacctgaag atggcctcgc
     2221 gcgtgcgcgg catcgacgcc atcatgggcg gccacaccca cgacggcatg ccggcgccgg
     2281 tcatcgtcaa gaatgccggc ggccagacct tggtcaccaa cgccggttcc aacggcaagt
     2341 acctcggcgt cctcgacttc gacgtcaaga acggcaagat cgccggctac cgctacaagc
     2401 tgctgccagt gttcgccaag ctgctgcccg ccgacccggc gatgcaggcg ctgatcgaca
     2461 aggtccgtgc gccttacctc ggcaagctca acgagcaact ggccatcagt gaaggcacgc
     2521 tgtaccgccg cggcaatttc aacggcactt tcgaccaggt catcgtcgat gcgctgatca
     2581 aggaaaagga cgccgaaatc gccttctcgc ccggtttccg ctggggcacc tcgctgctgc
     2641 ccgggcagcc gatcctgatg gagcacgtgc tcgaccagac ggcgatcacc tacccgtgga
     2701 cgacggtgag caacatgagc ggcgagatga tcaagaccgt gcgcggcttc ggctacaaac
     2761 tggagattac gcc
//
LOCUS       DAOSDX010000343         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12846_length_2766_cov_3.468093, whole genome shotgun sequence.
ACCESSION   DAOSDX010000343 DAOSDX010000000
VERSION     DAOSDX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12846_length_2766_cov_3.468093"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLN02_08275"
     CDS             complement(<1..808)
                     /locus_tag="PLN02_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOI52358.1"
                     /translation="MKRLLPRLIFGIFCTFWILGHVVQSWHIPLVALFDHYFYDVRVR
                     AFMPDTQDERIVIVDIDEKSLGELGRWPWKRSVLAELVRRLADDYAASVIGFDIVFAE
                     PDDSSGLPVLDAMAHGPLRDNVAYQRVLAGQRAGLDYDRRFAESLRGRPVVLGFYFSN
                     QAGASPGSRLPPPLLFEGQPSPGQFPVWPGFGGNLAAFQEAAASGGHFNPLMDSDGIT
                     RRVPLLGAYAGSYQPTLALAMSLMLLEAEKVRLFAPAAGAPVEWVDILGKR"
     gene            1201..>2766
                     /locus_tag="PLN02_08280"
     CDS             1201..>2766
                     /locus_tag="PLN02_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOI52359.1"
                     /translation="MNDLEKSRRRLELLYDVGISLSSEKHLPKLLEKIVQAAKCITNA
                     DGGTLYLLSEDGRSLRYEIVRTDSLDLAYGGSAPLPASGIFANLPLYRPDGSNNDAIV
                     AAYAALSASTVNIADAYEVKDFDFTGPRAFDARMGYRSRSFLTVPMKNHENELIGVLQ
                     LINAIDPETGKTTVFRETDQRLVESLASQAAVALSNRQLMHQMESLFEATIRMINTAI
                     AKKSAFTNSHCVRVPELTMLIAEAVCADTEGPLAGWTLNETEQHELRIAALMHDCGKI
                     TTPVHVIDKSRKLETIFDRIALIDTRFEVVRRDAKIRMLETIAAGADAAPAKAEHARL
                     LEEIAEDQALLQRSNLGSERMSDSDVERIKEIARKYRWTSADGVNHPFLDEDEIDNLT
                     IIAGTLTAPERQIINDHIVATINMLEELPWPKHLTNVPEIAGNHHERMDGQGYPRGVR
                     AGELSVQARILAIADIFEALTASDRPYKTPMKVSEALSILEKFKQRGHIDPDIFDSFV
                     RQQVHKRYAELFLT"
BASE COUNT          694 a          886 c          730 g          456 t
ORIGIN      
        1 gccgcttccc caggatatcg acccattcga cgggagcgcc ggctgccggg gcgaaaagcc
       61 tcactttttc cgcctcgagc agcatcaggc tcatcgccag cgccagcgtc ggctggtaag
      121 acccggcgta agcccccaac agaggaacgc gccgggtgat cccatcacta tccatcaacg
      181 ggttgaaatg cccgccgctc gccgcggcct cctgaaaggc cgcgagattt ccaccaaatc
      241 cgggccacac ggggaattgc cccggcgacg gctggccttc aaagagcaac ggcggcggca
      301 gtcggctacc gggcgaggct cctgcctggt ttgaaaaata gaaaccgaga acgaccggcc
      361 ggccgcgcaa ggattcggcg aacctccggt catagtcgag ccctgcccgc tgcccggcaa
      421 gcacccgctg atacgccaca ttgtcgcgca acgggccgtg cgccatggca tcgaggacag
      481 gcaggcctga actgtcgtcc ggctcggcaa aaacaatgtc gaaaccgatc accgacgccg
      541 cgtaatcatc ggccagacgg cgcaccagtt ccgccaggac gctgcgcttc cagggccagc
      601 gcccgagttc ccccaggctt ttttcgtcga tatcgacgat gacgatacgt tcatcctggg
      661 tatcgggcat gaaagcccgg acacggacat cgtaaaagta atgatcgaac agcgcgacca
      721 agggaatgtg ccaggactgg acgacgtgcc caagaatcca gaatgtgcag aatattccga
      781 atatcagccg gggcagcagt cgtttcatcg aaatatcacg ctttccagaa aaaatgcctt
      841 ggcaggcggt attgcgcaag catcgcaggg aatacactaa cacgagccgg ttcgcgatac
      901 actgaacccg aaatcaacag cgcccgaatc atcgatgaca cggcccagaa ggatattgat
      961 tgtttcaaga taacagcaaa atcattgaca tacaaaacaa atgaaccacc agcaaaatgt
     1021 tcttgcaaat cattgacgta atgtgtaatt caggacggaa cgaaacacga tcatatattc
     1081 aatacggttg ttttttaaaa taccgaggga aaggccctga taactcagtg aagcccggca
     1141 atgaaaaata aaaataaaga cacttgcgag gatattggaa taaaccgcgc ggagaatgcc
     1201 atgaacgacc tcgaaaaatc ccgacgccga ctcgaactcc tgtatgacgt aggtatttca
     1261 ctgagcagcg aaaagcattt accgaagctg ctggaaaaaa tcgtccaggc tgcaaaatgc
     1321 atcaccaacg ccgacggcgg aaccctttac ctgctctccg aagatggccg tagcctgcgc
     1381 tacgaaatcg tccgcaccga ctcgctcgat ctggcctacg gcggcagtgc ccccctcccc
     1441 gcatccggta tttttgccaa tcttcccctc tatcgcccgg acggctccaa caacgacgcg
     1501 atcgtcgccg cctacgccgc gctcagcgcc agcaccgtca atattgccga cgcctacgag
     1561 gtcaaggact tcgatttcac cggtccgcgg gctttcgacg cgcgcatggg ctaccgctcg
     1621 cggtctttcc tgacggtacc gatgaagaac cacgagaacg aattgatcgg cgttctccag
     1681 ttgatcaatg ccatcgatcc tgaaacgggc aagaccacgg ttttccgcga aaccgaccag
     1741 cgcctggtcg agtccctggc atcacaggcg gcggtcgcgc tatccaaccg gcagttgatg
     1801 caccagatgg aatcgctgtt cgaagcgacc atccgcatga tcaacacggc aatcgccaaa
     1861 aaatcggcat tcaccaacag ccactgcgtc cgggtaccgg aactgaccat gctgatcgcc
     1921 gaagccgtct gcgccgatac cgaaggcccg cttgccggct ggacgctcaa cgaaaccgaa
     1981 cagcacgagc tacgcatcgc cgccctgatg cacgattgcg gcaagatcac gacacctgtg
     2041 cacgtgatcg acaaatcgcg caaactggaa accatattcg accgcatcgc cctgatcgac
     2101 acccgcttcg aggtcgtccg gcgggatgcg aaaatccgca tgctggagac gattgccgcc
     2161 ggcgccgacg cagcgccagc caaagccgag cacgcgcgcc tgctcgagga aatcgccgaa
     2221 gaccaggcct tgctgcagcg ctccaacctg ggtagcgaac ggatgagcga cagcgatgtc
     2281 gagcggatca aggaaatcgc ccggaaatac cgctggacga gcgccgacgg cgtcaaccac
     2341 cccttcctcg atgaagacga gatcgacaac ctgaccatca tcgccggcac cctgacagcc
     2401 cccgagcggc agatcatcaa cgaccacatc gttgccacca tcaacatgct ggaagaactg
     2461 ccctggccca agcacctgac caacgttccg gaaatcgccg gcaaccacca cgaacggatg
     2521 gacggccagg gctatccacg cggcgtacgg gcgggtgaac tcagcgtcca ggcgcgcatc
     2581 ctggcgattg ccgacatttt tgaggcactt accgcctccg accgccccta caagacgccg
     2641 atgaaggtca gcgaagcgct gtccattctg gagaaattca agcaacgcgg ccacatcgac
     2701 ccggatatct tcgacagctt cgtgcgccag caggtgcaca aacgctacgc cgaactcttc
     2761 ctcacc
//
LOCUS       DAOSDX010000344         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12988_length_2744_cov_2.991819, whole genome shotgun sequence.
ACCESSION   DAOSDX010000344 DAOSDX010000000
VERSION     DAOSDX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12988_length_2744_cov_2.991819"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>353)
                     /locus_tag="PLN02_08285"
     CDS             complement(<1..>353)
                     /locus_tag="PLN02_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOI52360.1"
                     /translation="IMAGMAAALTEEDMKNVAAWFASQKLKPESAKNEASLALGKKIW
                     RQGDFRKGVPACAGCHGPAGSGMPAQYPRLAGQFAEYTEAQLVAFRAGTRANDPESMM
                     RTIAEKMTDADMKAV"
     assembly_gap    354..363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<364..603)
                     /locus_tag="PLN02_08290"
     CDS             complement(<364..603)
                     /locus_tag="PLN02_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOI52361.1"
                     /translation="MIRSAAMAILFATSFVTHASEQATAKADPAKGKIVAETICVACH
                     GADGNSMSSANPHLAGQIEQYIYKQLSNFKTVDGNP"
     gene            712..1410
                     /gene="yihA"
                     /locus_tag="PLN02_08295"
     CDS             712..1410
                     /gene="yihA"
                     /locus_tag="PLN02_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOI52362.1"
                     /translation="MPLFQNAVFHTTVANLRDLPVDATCEVAFAGRSNAGKSSAINTL
                     AGRVRLAFVSKTPGRTQHLNYFTLAPGKYFVDLPGYGFAKAPEDIRAQWEGLIGPYLS
                     RRGPLVGLVVIMDCRRPFTDLDRNLIEWFKPTGRPIHLLLSKADKLSRQEQTKVLRAV
                     KAEIATWVGGTYSAQLFSSLKKTGIEEAEAVLAGWLGIESPKKAVTENKGPPDKGNPG
                     AKMPQQCQGTRSGR"
     gene            1542..2510
                     /gene="hemB"
                     /locus_tag="PLN02_08300"
     CDS             1542..2510
                     /gene="hemB"
                     /locus_tag="PLN02_08300"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOI52363.1"
                     /translation="MRRMRRDDFSRRLMRESVLTPDDFIYPVFVLEGGGRTEKVVSMP
                     GVERQSLDVLLRTAERTVKLGIPALALFPVIDAPLKTLGAEEAYNPEGLVPRTVRALK
                     REFPELGVITDVALDPYTSHGQDGLIDDTGYVLNDETLDVLVRQALCHAEAGADVVAP
                     SDMMDGRIGRIRETLERNGQIYTRILAYSAKYASAFYGPFRDAVGSAGNLGKGNKYTY
                     QMDPANGDEALREVAQDIEEGADMVMVKPGMPYLDIVRRVKDEFGVPTYAYQVSGEYA
                     MLKAAALNGWLDERAVVLESLLAFKRAGADGILTYFALDAAEYLRG"
     gene            complement(2563..>2744)
                     /locus_tag="PLN02_08305"
     CDS             complement(2563..>2744)
                     /locus_tag="PLN02_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HOI52364.1"
                     /translation="QEKFKADADFGALANLLRCYVVGRWEQYARSGFSREPLAQWPQQ
                     WPMLYFACLSQAGEA"
BASE COUNT          495 a          786 c          894 g          559 t
ORIGIN      
        1 acaccgcctt catgtcggcg tcggtcatct tttcggcgat ggtgcgcatc atgctttccg
       61 gatcgttggc gcgggtgccg gcgcggaaag ccaccagttg cgcttcggtg tattcggcaa
      121 actggccagc caggcgcgga tactgggccg gcatgccgga gccggccggg ccgtggcaac
      181 cggcgcaggc gggaacgccc ttgcggaagt cgccctgacg ccagattttc ttgcccagcg
      241 cgaggctggc ttcgttcttc gccgattccg gcttcagttt ctggctggcg aaccaagcgg
      301 cgacattctt catgtcttct tcggtcagcg ccgcggccat gccggccatg atgnnnnnnn
      361 nnnggggttg ccgtcgactg tcttgaagtt ggacaactgc ttgtaaatgt actgttcgat
      421 ctggccggcc agatgcgggt tggcggaact catgctgttg ccgtcggcac cgtggcaggc
      481 gacgcagatg gtttcggcga cgatttttcc tttggcagga tcggccttgg ccgttgcttg
      541 ctcggaagcg tgggtgacaa aactggtggc aaacaggatt gccattgccg cagaacggat
      601 cattttcgaa ctcctagtgc gcatgatggg gcgctttggc cggaacggaa gttacaaacc
      661 tgttattcta tattagtttc ccttcaacta ggcgggtctt ccgcgttttt tatgcctctt
      721 ttccagaacg cggtttttca tacgaccgta gccaatctgc gcgacctgcc agtcgatgcc
      781 acttgcgaag tggcgtttgc cggacgctcg aatgccggca agtcgtcagc gatcaacacc
      841 ctggcgggac gggtgcgtct tgccttcgtc agcaagacgc cggggcgtac ccagcacctc
      901 aattacttca cgctggcacc gggaaagtat ttcgttgacc tgcctggcta tggttttgcc
      961 aaggcgcccg aagatattcg ggcgcagtgg gaaggactga tcggccccta tctcagccgg
     1021 cgcgggccgc tggtcggctt ggtggtgatc atggattgcc ggcgaccatt caccgacctc
     1081 gaccgcaacc tgatcgagtg gttcaagccc accgggcggc cgattcatct cctgctgtcg
     1141 aaggccgaca aattgtcgcg gcaggagcag accaaggtac tgcgtgcggt gaaggcggaa
     1201 atcgctacct gggtgggcgg aacctatagc gcccagctct tttccagtct gaagaagacc
     1261 gggatcgagg aggcggaagc cgttcttgcc ggctggttgg gcatcgagtc gccgaaaaag
     1321 gctgtgacgg aaaacaaagg gcccccggac aaggggaatc cgggggccaa aatgcctcaa
     1381 cagtgtcaag gcacccgctc agggaggtga agcgggagac ggcgcgagcc atcgtgcctt
     1441 tctgaccgcg gggtttgaaa aaaggttccg cgcgggttta aatttttatc tttatttttt
     1501 gcagggatgt cgacatggct ggcagttttc ccggaatccg gatgcgccgg atgcggcgcg
     1561 acgatttttc gcgtcgcctg atgcgcgaat cggtactgac cccggacgat ttcatttacc
     1621 cggtcttcgt tctcgaaggc gggggccgta cagagaaggt ggtttcgatg cccggcgtcg
     1681 agcggcaatc gctcgatgtc ctgttgcgta ccgcggagcg cacggtcaag ctgggtattc
     1741 cagcgctggc gctgtttccg gtgatcgatg cgccgctgaa aacgctcggc gcggaggaag
     1801 cctacaaccc ggaaggtttg gtgccgcgta cggtacgcgc cctcaagcgc gagtttcctg
     1861 agttgggcgt gatcaccgac gtcgctcttg atccctacac cagccatggt caggacggcc
     1921 tgatcgacga caccggctat gtgctcaatg acgagacgct cgacgttcta gttcgccagg
     1981 cgctgtgcca tgccgaagcc ggtgccgacg tggtggcgcc gtcggacatg atggatggac
     2041 ggatcggccg catccgcgaa acgctggaac gcaacggcca gatttacacg cgcatcctgg
     2101 cctattcggc caagtacgct tccgccttct acggcccctt ccgcgacgcg gtcggctcgg
     2161 ccggcaacct tggcaaaggc aacaagtaca cctaccagat ggacccggcc aacggcgacg
     2221 aggcgctgcg tgaagtggcg caggatatcg aggaaggtgc cgacatggtc atggtcaagc
     2281 cgggcatgcc gtatctggat atcgtccgcc gggtcaagga cgagttcggc gtgccgacct
     2341 atgcctacca ggtcagcggc gagtacgcga tgctcaaggc ggcggcattg aacggctggc
     2401 ttgacgagcg ggcggtggtg ctggagagcc tgctggcgtt caagcgcgcc ggcgccgacg
     2461 gcatcctgac ctatttcgcg ctcgatgcgg cggaatacct gaggggctga tcaggatttt
     2521 aaaagcaagc taaaagggcg gctggtgggc cgcccttggc gcttaagcct cgccggcctg
     2581 cgacaggcag gcgaagtaaa gcatcggcca ctgctgcggc cattgcgcca gtggttcgcg
     2641 gctgaagccg gagcgggcgt attgctccca gcggccgacg acgtagcagc gcaagaggtt
     2701 ggccagcgcc ccgaaatcgg cgtcggcctt gaacttctcc tgcg
//
LOCUS       DAOSDX010000345         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_12989_length_2744_cov_2.919673, whole genome shotgun sequence.
ACCESSION   DAOSDX010000345 DAOSDX010000000
VERSION     DAOSDX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12989_length_2744_cov_2.919673"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..615)
                     /locus_tag="PLN02_08310"
     CDS             complement(211..615)
                     /locus_tag="PLN02_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOI52365.1"
                     /translation="MKRSARSLTIQAEIDEITIMASHKKIDRKTEEAVRRFLQLIAGR
                     YEMTGAIVYGSRARGTHRRDSDADVAVLLKGEHQRILPTTLAMANFAYDVLLETGINI
                     SPLPVWQDEWEHPENAANPSLLSNIAREGIRI"
     gene            complement(612..2129)
                     /locus_tag="PLN02_08315"
     CDS             complement(612..2129)
                     /locus_tag="PLN02_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HOI52366.1"
                     /translation="MPLDYASLTALRTYHPAWRLLRSDHAALVASFLHRVFVAPNQRV
                     MAAADLAELLEDELYALRLQLGDSAFPKAAIDYLNDWAAPDKGWLRKFYKSGTDEAQF
                     DLTPATEKALAWLAQLAERQFVGTESRLLTLFDLLKQMREGSETDPAARIAELQRKRN
                     EIDAEIARVVAGDLPMLDDTALRERFQQFIQGARELLGDFREVEHNFRQLDRRVRERI
                     ALWEGGKGALLEEIMGERDAIADSDQGKSFRAFWDFLLSSRRQEELGDLLEQVLALPA
                     VAALHPDARTRRVHYDWLEAGEHTQRTVASLSQQLRRFLDDQAWLENRRIMDILHGIE
                     SKALALRAHWGDGWPPAGPVMEIAEARADIDLAMERPLFTPAVKPLLSSLALQAGDED
                     IDPAALFDQVLVDPAQLVRHIRHALQDKTQTTLSELVASRPLTHGLAELVTYLHLGSD
                     TFNAVVDEDHPEVIHWQARTETGQSITRSARLPRVIFLAAQKTESHAQTHQQESF"
     gene            complement(2136..2543)
                     /locus_tag="PLN02_08320"
     CDS             complement(2136..2543)
                     /locus_tag="PLN02_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOI52367.1"
                     /translation="MIYLLDTHILIYLIKKQPPDIARRIDALPEEDSLGMSFVTWAEL
                     LKGAERSTRKSDVLRRLEALARQVPVRYPGGPAICRHYAEQATRLKDNGTPIGANDLW
                     IACHALAENATLVTHDTREFVRVSGLKIEDWAA"
     gene            complement(2540..>2744)
                     /locus_tag="PLN02_08325"
     CDS             complement(2540..>2744)
                     /locus_tag="PLN02_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOI52368.1"
                     /translation="AEFRLDADRVSISRDEAGDLIIHPLPTPRGTALLQALRALGEVD
                     EAFIQALESGQASPLPAPERETL"
BASE COUNT          429 a          809 c          958 g          548 t
ORIGIN      
        1 ggcgcggtag agcaccccct tgagcaatgc gatcagcagg gccgagaggt cgggggccgg
       61 ggtttcggca ggagaggctt ccatcaggtt ttgctggggt tcaggtgccg gactcgtcat
      121 ggggagccag cagggggcca agcgtcggtt cgttacgcac tgcgttgatg aactgctcgg
      181 cttgttcgac gattacgcgg gcgtcacttt tcaaatccgg ataccttccc tggcgatgtt
      241 actcaacagc gagggattcg ccgcgttctc cgggtgttcc cactcgtcct gccagacggg
      301 cagtggcgag atattgatgc ctgtctcaag caggacgtcg taggcgaaat tcgccatggc
      361 cagcgtggtt ggcaaaatgc gctggtgctc gcccttcagg agtacggcga catcggcatc
      421 gctatcccgg cggtgcgtgc ctcgagcccg gctgccgtag acgattgcgc ctgtcatctc
      481 gtatcgaccg gcgatcaact gcaggaagcg gcgaaccgct tcttcagtct tccgatcgat
      541 tttcttgtga cttgccatga tcgttatctc gtcgatttca gcctggatag taagcgatcg
      601 ggcagatcgt ttcaaaaact ctcttgttga tgtgtttgtg catgactttc tgttttctgt
      661 gcggccagga aaatcacccg tggcaaccgg gccgagcggg tgattgattg cccagtttcg
      721 gtccgggcct gccagtggat gacttccggg tggtcttcgt cgacgacggc gttgaaggtg
      781 tcgctgccca gatgcaggta ggtcaccagt tcggccaggc cgtgcgtcag cggtcggctg
      841 gcgaccagtt cgctcagggt ggtctgggtc ttgtcctgca gggcgtggcg gatgtggcgg
      901 acgagttgcg ccgggtcgac cagtacctgg tcgaagaggg ccgcagggtc gatgtcttcg
      961 tccccggctt gcagggcgag gctgctcagg agcggcttca cggcgggagt gaatagcgga
     1021 cgctccatcg ccagatcgat gtcggcacgg gcttcggcga tttccatcac ggggccggcc
     1081 ggtggccagc catcgcccca gtgcgcgcgc agcgccagtg ccttgctttc gatgccatgc
     1141 aggatgtcca tgatgcggcg gttttccagc caggcctgat cgtcgaggaa gcggcgcaat
     1201 tgctgcgaca gcgaagccac ggtgcgctgg gtgtgttcgc cggcttccag ccagtcgtaa
     1261 tggacgcggc gggtgcgggc gtcggggtgc agggcggcga ccgccggcag ggccagcacc
     1321 tgttcgagca aatcgccgag ttcctcctgg cggcggctcg acagcaggaa atcccagaag
     1381 gcgcggaagc ttttgccctg gtcggaatcg gcgatggcgt cgcgttcgcc catgatctct
     1441 tcgagcaggg cgcccttgcc gccttcccac agggcgatgc gctcgcggac gcggcggtcg
     1501 agctggcgga aattgtgttc gacctcgcgg aaatcgccga gcagttcgcg cgcgccctgg
     1561 atgaactgct ggaaacgctc gcgcagcgcc gtgtcatcga gcatcggcag gtcgccggcg
     1621 acgacgcggg cgatctcggc gtcgatctcg ttgcgtttgc gttgtagttc ggcaatgcgc
     1681 gcggcgggat cggtttcgct gccttcgcgc atctgcttga gcagatcgaa cagcgtcagc
     1741 aggcgcgatt cggtgccgac gaactgcctt tcggccagtt gcgccagcca ggcgagggct
     1801 ttttcggtgg ccggcgtcag gtcgaactgg gcttcgtcgg tgccgctctt gtagaacttg
     1861 cgcaaccagc ccttgtcggg cgccgcccag tcgttcaggt agtcgatggc cgctttggga
     1921 aaggcggagt cgcccagttg caggcgcagg gcatacaact cgtcttccag cagttcggcc
     1981 aggtcggcgg cggccatgac gcgctggttg ggagccacga acacgcggtg cagaaagctc
     2041 gccaccagtg ccgcgtggtc ggagcgcagc aggcgccagg ccgggtggta ggtgcgtagg
     2101 gcagtgaggc tggcgtaatc gagcggcatt acggtctacg ccgcccagtc ttcgattttc
     2161 aggccgctca cgcggacgaa ctcgcgcgtg tcatgcgtga ccagggtcgc gttctcggcc
     2221 agcgcgtgac aggcaatcca caaatcgttg gcgccgatgg gcgtgccatt gtccttgagg
     2281 cgggtcgcct gttcggcata gtggcggcag atggccgggc cgccggggta acgcacgggc
     2341 acctggcggg ccagcgcttc cagccggcgc aggacatcgc tcttgcgggt actgcgctcg
     2401 gcacccttga gcagttccgc ccaggtgacg aaggacatgc ccaggctgtc ttcttcgggc
     2461 agggcatcga tgcgtcgcgc gatgtccggc ggctgcttct tgatcaggta gatcaggata
     2521 tgggtatcga gcaggtagat cacagcgttt cgcgctccgg cgccggcaag ggcgatgcct
     2581 ggccgctttc cagcgcctgg ataaacgctt cgtcaacctc gcccaatgcc cgcagggcct
     2641 gcaacagcgc cgtaccgcgt ggcgtcggca gcgggtgaat gatcaggtcg ccggcctcgt
     2701 cgcgggagat gctgactcga tcggcatcga gacggaactc ggcc
//
LOCUS       DAOSDX010000346         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13000_length_2741_cov_3.213328, whole genome shotgun sequence.
ACCESSION   DAOSDX010000346 DAOSDX010000000
VERSION     DAOSDX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13000_length_2741_cov_3.213328"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..334)
                     /locus_tag="PLN02_08330"
     CDS             complement(104..334)
                     /locus_tag="PLN02_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52369.1"
                     /translation="MQTKKFNINELTLEDMQPKQASRHDPSNVGRKCLKCEHIRLASE
                     SHIPAYECPKCGAVYDKIEQLVSARPMGKLAR"
     gene            complement(383..814)
                     /locus_tag="PLN02_08335"
     CDS             complement(383..814)
                     /locus_tag="PLN02_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HOI52370.1"
                     /translation="MNQTIHLQNRLAKAHHLRGDGGFSLVELMIVVAVIGILASIAVP
                     SYQAYVRQSVRTQAQGCVSQIAQALERRYSTALSYAGAMPVTGCTTEGGLNTRYTITA
                     NIQARSYVLTATPSGDQVKDTCGTMTLNNQATKTAAKTGCW"
     gene            complement(817..>2741)
                     /locus_tag="PLN02_08340"
     CDS             complement(817..>2741)
                     /locus_tag="PLN02_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOI52371.1"
                     /translation="GEFIVASNPDEFTKALGDTLNAIKNETKSAASGGSSSTRIEAGA
                     KAIFSQYTSGSWNGNLLAYNINETTGLQDTTTPAWTAESMLPAWGSRSIYVSKDGASV
                     PFRYSNLNAAQQTALGSQQVVDYLRGDRSQEQPSGSLRARAGVLPAFVNSQLVYVGPP
                     SQTAYFGNASFTGASQYAAYATSKQSRTPILYVAGNNGMLHAFNGNTGAEVYAFLPNF
                     SIMHPSASATQKTLKLYTDPKYGLNDSADADWETYKHRYILDGELTVADVYLAGAWKT
                     ILVGTQGRGGTGVFALDVTDPTDIKFLWEKTSSDSAALGNNLGKPIIAQVASGDWRVV
                     LGNGPNSTGDKAQLIMIKLSDGSISTVDTGVGENNGLSGVNLWDADKDGFFETAYAGD
                     LKGNLWRFKGLGGTPSFAKLFATRNARPITAMPLIVRNQKTNKTWVFFGTGQNLNKVD
                     QENLDVQSWYGMMDDGSEVASNKLRARAIGTSGTIGARSARILESGTEAEIISAGQLG
                     WYIDFPANGERMITPNSLLGGALFGTTYTPDNGDTCAPDGKSSLWAINPFSGARLNQG
                     IFDVNGDGVVNDSDKVNGVFPSALDGLSPITSGQPPITVGKDGKFSVHLPGESIGGKL
                     PVGVLGRQSWREIIGQ"
BASE COUNT          482 a          752 c          871 g          636 t
ORIGIN      
        1 gcggtggggc agttgcaggg cgtggaagaa ggcgtttcgt tcgccctgcc gaaagaactt
       61 gcctgaaggc cctgagggat caggagtatg gacagacagt cgttcagcgt gccagtttgc
      121 ccatgggacg agcgctgacc agttgctcga tcttgtcgta aacagcaccg catttcgggc
      181 actcataggc cggaatgtgg ctttccgaag cgagccggat gtgctcgcat ttgagacact
      241 tgcgaccgac gttcgaggga tcatgccgag aagcctgctt cggttgcatg tcctcgagcg
      301 tcagttcgtt gatgttgaat ttcttggttt gcatgttgct ttccggtcag aaaaaaatcc
      361 cggaacattg tagcttcccc gcttaccagc aaccagtctt ggcggctgtt ttggtggcct
      421 ggttgttcag ggtcatcgtg ccgcaagtgt ccttgacttg gtcccccgat ggcgtcgcag
      481 tcagcacata gcttctcgcc tgaatgttgg ccgtgatcgt gtagcgcgtg ttcaatcccc
      541 cttctgtggt gcatcccgtt acaggcatcg cgcccgcata gctgagcgcg gtggaatagc
      601 gtctttccag tgcctgggca atctgtgaaa cgcatccctg agcctgggta cggacgctct
      661 gccgaacata ggcctgatag ctcggcaccg caatactggc aaggatgccg atgacagcga
      721 cgacgatcat gagttcaacc agagagaatc ccccgtcgcc gcgtaggtga tgggccttgg
      781 ctagcctgtt ctgtaagtga atggtttggt tcatgtttat tgtccgatga tttcacgcca
      841 ggattgacgt cccaatacgc cgaccggaag tttgccaccg atcgattcgc cggggagatg
      901 gacggagaat tttccatcct tgcccaccgt gatgggaggc tgtccgctgg tgatcggcga
      961 gagtccatcg agcgcggacg ggaaaacgcc gttaaccttg tccgagtcat tgacgacccc
     1021 gtcgccattg acgtcgaaga tgccctggtt gagtcgcgcg ccactgaacg ggttgattgc
     1081 ccacaacgaa ctcttgccat caggggcgca agtgtcgccg ttgtccggtg tgtaggtcgt
     1141 gccgaagagc gcacctccca gcaggctgtt cggggtgatc atgcgttcgc cgttggcggg
     1201 gaagtcaatg taccagccga gttgtccggc gctgatgatt tccgcctccg taccggattc
     1261 cagtattctg gctgaacgcg caccaatggt gccggaagtg ccgattgcgc gagcccgcaa
     1321 cttgttgctg gcgacctcgc tgccgtcgtc catcatgccg taccaactct ggacatccag
     1381 attttcctgg tcgaccttgt tgagattttg gccggtaccg aagaaaaccc aggtcttgtt
     1441 ggttttctga ttgcgtacga tcagcggcat ggcggtgatc gggcgggcat tgcgggtggc
     1501 gaacaatttg gcaaagcttg gcgtcccgcc gagtcccttg aagcgccaca ggttcccctt
     1561 caggtcgccg gcataggctg tttcgaagaa accgtccttg tccgcatccc acagattgac
     1621 gccggacaga ccgttgtttt cgccgacgcc ggtatctacc gtcgatatgc tgccgtcgga
     1681 aagcttgatc atgatcaatt gcgccttgtc gccggtgctg ttgggaccgt tgccgagaac
     1741 gacgcgccag tcgccgctcg ccacctgggc aatgatcggt ttgcccagat tgttgcccaa
     1801 ggcagcgctg tcgctgctgg ttttttccca gaggaacttg atgtctgtcg ggtcggtcac
     1861 gtcgagggcg aatacgccgg tgccgccgcg tccttgagta ccgaccagaa tggtcttcca
     1921 ggcaccggcg agatagacat cggccacggt cagttcgcca tccagaatat agcggtgctt
     1981 gtaggtttcc cagtcggcat ccgcggaatc gttcaggccg tatttcgggt cggtatagag
     2041 cttcagcgtc ttctgggtgg ctgaggcgct cgggtgcatt atcgagaaat tgggcaggaa
     2101 ggcatagact tccgcaccgg tattgccgtt gaaagcgtgc agcatgccat tgttgcccgc
     2161 gacgtagagg atcggggtgc gactctgttt gctggtggcg taagccgcat actggctggc
     2221 gccggtgaag ctggcattgc cgaagtacgc cgtctggctg ggcgggccga catagaccag
     2281 ttgcgagttg acgaaggcgg gcaggacgcc ggcgcgggcg cgcaagctac ccgagggttg
     2341 ttcctggctg cggtcgccgc gcaggtagtc gacgacttgc tgtgacccca gtgccgtttg
     2401 ctgcgcggcg ttcaggttgc tgtagcggaa cggtaccgaa gcgccgtcct tgctgacata
     2461 gatgctgcgc gacccccagg cgggaagcat gctttccgcc gtccaggcgg gagtcgtggt
     2521 gtcctgcagg ccggtagttt cattgatgtt gtaggcgagg aggttgccgt tccaggagcc
     2581 ggaagtgtat tggctgaaaa tggccttggc gccagcttcg atccgggtgg aactggagcc
     2641 gccgctggcc gcgctcttgg tttcgttctt gatcgcgttc agcgtatcgc ccagcgcttt
     2701 ggtgaattca tccgggttgg aggcgacgat gaattcgccg c
//
LOCUS       DAOSDX010000347         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13086_length_2729_cov_3.345550, whole genome shotgun sequence.
ACCESSION   DAOSDX010000347 DAOSDX010000000
VERSION     DAOSDX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13086_length_2729_cov_3.345550"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLN02_08345"
     CDS             <1..350
                     /locus_tag="PLN02_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOI52372.1"
                     /translation="DAQITLIATGSEVKLALDAQAALAGEGIAARVVSMPCTNVFDRQ
                     SDDYKASVLGTCKKRIAIEAAHPDFWRKYVGLHGAVIGIDRFGESAPAGQLFELFGFT
                     VGNVVATAKSLLS"
     gene            386..1414
                     /gene="gap"
                     /locus_tag="PLN02_08350"
     CDS             386..1414
                     /gene="gap"
                     /locus_tag="PLN02_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOI52373.1"
                     /translation="MAIKVAINGFGRIGRCTLRAIYEQGLQNEFDVVAINASGDLATN
                     AHLLKYDTTHGRFTTSVETEGENCIIIDGKKIAFYSTKNPKDINWADHGVDVLLECTG
                     AYTSKAKAQALLEQGAKRVLISAPGGDDVDGTIVMGVNEGILTQAMSVVSNASCTTNC
                     LAPVAKVLSDNIGIKSGLMTTVHAYTNDQVTVDVRHKDLRRARAAAANIIPTKTGAAK
                     AVGLVLPQLVGKFDGFALRVPTINVSLVDLTFTAERATTKDEINALMTAAANGPLKGI
                     MAVNTEPLVSSDFNHTTVSSTFDATQTRVLKAEDGSTLVKVLAWYDNEWGYSCRMLDA
                     ARAWMAAK"
     gene            1617..>2729
                     /locus_tag="PLN02_08355"
     CDS             1617..>2729
                     /locus_tag="PLN02_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52374.1"
                     /translation="MSWFDNLSLRYKLLINFLASGGILIAAIVFSVVELRSAGRDMAE
                     LAGNSLPSIQAIGEISQLRLRYRVRSLEYMLPDTPEAKAKLAKDMGKLDGMLREAFAR
                     YEKLISSDKEREVYKAAIAAADDYKASVEKAVALTVAGDEAGAQQLRKTEWVKNANKL
                     RDRTDELTDINRQAADALSEQTQKDLASGLTGAITALIGGTLMALLVSYVVAQRMGHQ
                     LDQAVAATERIAEGNLTGDVPGGGADEIGKLLRAVAHMQLSLRSTIGESLRGAGQVLE
                     ASKALNHSVQMIDESASIQSSAASAIAANVEELTVSINHVSDSTGEAARLAGESDKQA
                     SHGRQAIDALVDRINQVADVVREASEQITNLKNESEK"
BASE COUNT          588 a          928 c          779 g          434 t
ORIGIN      
        1 ccgacgcgca gatcacgctg atcgccaccg gctccgaggt caagctggcg ctcgacgccc
       61 aggccgcgct ggccggcgaa ggcatcgccg cgcgcgtcgt gtcgatgccc tgcaccaatg
      121 tcttcgaccg ccagagcgac gactacaagg catcggtgct tggcacctgc aagaagcgca
      181 tcgccatcga agcggcacat ccggatttct ggcgcaagta cgtcggcctg cacggcgcgg
      241 tcatcggtat cgaccgcttc ggcgaatcgg caccggccgg ccagctcttc gaactgttcg
      301 gcttcaccgt cggcaacgtc gtcgccacgg cgaaaagcct gttgtcctga tttcaataca
      361 tccaacgcag agaggaaata gatccatggc tatcaaggtt gcaatcaacg gtttcggtcg
      421 tatcggtcgc tgcacgctgc gcgccattta cgaacaagga ttgcagaacg aattcgacgt
      481 tgtcgcgatc aacgcctccg gcgacctggc gaccaacgcc cacctgctga agtacgacac
      541 gacgcacggc cggttcacga ccagcgtcga aaccgaaggt gaaaactgca tcatcatcga
      601 cggcaagaag atcgccttct actcgaccaa gaaccccaag gacatcaact gggccgacca
      661 cggcgtcgac gtcctgctcg aatgcaccgg cgcctacacc tccaaggcca aggcccaggc
      721 actgctcgaa caaggcgcca agcgcgtgct gatctccgcc ccgggcggtg acgacgtcga
      781 cggcaccatc gtcatgggcg tcaacgaagg catcctgacc caggcgatga gcgtcgtctc
      841 caacgcctcg tgcaccacca actgcctggc cccggtggcc aaggtgctgt ccgacaacat
      901 cggcatcaag tccggcctga tgaccacggt gcacgcctac accaacgacc aggtcacggt
      961 ggacgtccgc cacaaggacc tgcgccgcgc ccgtgccgcc gccgccaaca tcatcccgac
     1021 caagaccggc gccgccaagg ccgtcggcct ggtgctgccg caactggtcg gcaagttcga
     1081 cggtttcgcg ctgcgcgtgc cgaccatcaa cgtgtccctc gttgatttga ccttcaccgc
     1141 cgaacgcgcc accaccaagg acgaaatcaa cgccctgatg accgctgccg ccaacggccc
     1201 gttgaagggc atcatggcgg tcaacaccga gccgctggtg tcgtccgact tcaaccacac
     1261 caccgtctcg tcgaccttcg acgcgaccca gacccgcgtc ctcaaggccg aagacggctc
     1321 gaccctggtc aaggtgctgg cctggtacga caacgagtgg ggttactcct gccgcatgct
     1381 cgacgccgcc cgcgcctgga tggctgccaa gtaaacgaag caggctcaag gaaaccgccg
     1441 gcaagccggc ggtttttttt cgcccggtta atgccaagtg cttataatcg attaagccag
     1501 atttaatcca tttactgcac tatcagtctg caaatccatc gtcccctcgc attgcccaaa
     1561 aatagagcaa ttgcaatgca gcaagctgac aataatttca cattccggga ggcgtcatga
     1621 gctggttcga caatctttca ctgcgttaca agctgctgat caactttctt gccagcggcg
     1681 gcatcctgat cgcggcgatc gtcttctccg tcgtcgaatt gcgttcggca ggcagggata
     1741 tggccgagct tgccggcaac agcctgccct cgatccaggc gatcggggaa atcagccagc
     1801 tccgcctgcg ttatcgcgta cgcagcctgg aatacatgtt gcccgacacc ccggaagcca
     1861 aggccaagct ggccaaggac atggggaagc tcgacggtat gttgcgcgag gccttcgcca
     1921 ggtacgaaaa actgatttcc agcgacaagg aaagggaagt ctacaaggcc gccatagctg
     1981 ccgccgacga ctacaaggcc agcgtcgaga aggcggttgc gctgaccgtg gcgggtgatg
     2041 aagccggcgc ccagcaactg cgcaaaaccg agtgggtgaa aaacgccaac aagctgcgcg
     2101 accgcaccga cgagttgacc gacatcaacc gccaggcggc cgacgcgctg tccgaacaga
     2161 cccagaagga cctggccagc ggactgaccg gcgccatcac cgcgctgatc ggcggcacct
     2221 tgatggccct cctcgtttcc tatgtcgtcg cccagcgcat gggccatcag cttgaccagg
     2281 ccgtcgccgc caccgagcgc atcgccgagg gcaacctgac cggcgacgta ccgggcggcg
     2341 gcgccgacga aatcggcaag ctgctgcgcg cggtcgccca catgcagctc tcgctgcgca
     2401 gcaccatcgg cgaatcgcta cgcggggccg gccaggtgct cgaagcctcc aaagccctca
     2461 accactcggt gcagatgatc gacgaatcgg cgtcgatcca gagctccgcc gcctcggcga
     2521 tcgccgccaa cgtcgaggaa ctgacggtgt cgatcaacca cgtttccgac agcaccggcg
     2581 aagccgcccg cctcgccggg gaatcggaca agcaagccag ccatggtcgg caggccatcg
     2641 acgcactggt cgaccgcatc aatcaggtgg ccgatgtcgt acgcgaggca tccgagcaga
     2701 tcaccaatct caaaaacgaa tccgagaag
//
LOCUS       DAOSDX010000348         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13104_length_2726_cov_3.058405, whole genome shotgun sequence.
ACCESSION   DAOSDX010000348 DAOSDX010000000
VERSION     DAOSDX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13104_length_2726_cov_3.058405"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLN02_08360"
     CDS             <1..324
                     /locus_tag="PLN02_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOI52375.1"
                     /translation="YDYDEIDYMTDINFRQIPPAPDFETEMSGEVWYPVHRGDVFPEE
                     FATFLLGSPQVRKLFMKHHKDLLTPAFWQDAQAKIRAGHVEDFFPYPQELRFVNSHPG
                     QDENT"
     gene            465..1592
                     /locus_tag="PLN02_08365"
     CDS             465..1592
                     /locus_tag="PLN02_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOI52376.1"
                     /translation="MPLTLAVARERSDGESRTALVPETAKKFAALGARLRMEQSAGVQ
                     SHFLDPDYIGVDFMPSLEGSYAGADLILRVTPPSLAEIAAMPEGAVLIGLLKPYESKE
                     RLAALNARKITAFSLELLPRISRAQSMDALSSQGACAGYQCGLIAAARCTKFFPMLTT
                     AAGTIRPARVLVIGAGVAGLQAIATCKRLGAMVEAYDVRAAAKEQIESLGAKFVDTGV
                     SADGAGGYARELTAEEKAAQAEKLAKAVAAADVVITTAAIPGKKAPVIVTRDMVGRMK
                     YGAIIVDMAAESGGNCELTQPGEHVVANDVNIHGPLNLPSRMPTHASELYAKNIYNFL
                     SPWIKDGALNIDWSDEVVAGTVLCRDGATVHATVKQILGDA"
     gene            1592..1879
                     /locus_tag="PLN02_08370"
     CDS             1592..1879
                     /locus_tag="PLN02_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOI52377.1"
                     /translation="MDGLLALYIFMLAAFTGYEIIAKVPVILHTPLMSGSNFVHGVVV
                     VGAMIALGHAETPLEQAIGFFAVALGAANAAGGYVVTERMLGMFKKKEGTQ"
     gene            1876..>2726
                     /locus_tag="PLN02_08375"
     CDS             1876..>2726
                     /locus_tag="PLN02_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOI52378.1"
                     /translation="MTAPFYVQGAWFVGAALFIFGLKGMSSPASARRGIVIAGYGMLI
                     AIAATFLIPGLHNLGLIALALVLGGGAAWISGQKVKMTDMPQMVAIYNGMGGGAAAAI
                     AAIEFARGDAHGLVTTTLAVLGALIGAVSFTGSCVAWAKLQGVLRKAYRLPAQNAVNV
                     TLAAVAIGLGLSMIALAPAQPQLIIGFFVVALVLGLIVTLPIGGADMPVVISLFNAFT
                     GLAVGFEGYVLGNPALIIAGIVVGAAGTLLTQLMAKAMNRPLSNILFTPMVASGPGEA
                     ISGSMKEV"
BASE COUNT          475 a          959 c          828 g          464 t
ORIGIN      
        1 tacgattacg acgagatcga ctacatgacc gacatcaact tccgccagat tccgccggca
       61 ccggatttcg aaaccgagat gtcgggcgag gtctggtacc cggttcaccg cggcgacgtc
      121 tttcccgagg aattcgccac cttcctgctc ggttcaccgc aggtgcgcaa gctcttcatg
      181 aaacaccaca aagacctgct gaccccggcc ttctggcagg acgcccaagc caagatccgg
      241 gccggccacg tcgaggattt cttcccctat ccgcaggaat tgcgcttcgt caacagccat
      301 cccggccagg atgaaaacac ctgatgccca cgcctttcga cagcaaccgg taagtgattg
      361 ctgaccagtc acgtcagcaa tactgattgt ttcaacatta acaatccact tatcttagcc
      421 aggaaaaatt aaccctcgaa tgactttcag gcaaggagat caaaatgccg ctgaccctgg
      481 cggttgcccg tgaacgcagc gacggcgaaa gccgcaccgc gctcgttccc gagacagcca
      541 agaagttcgc cgcacttggc gcccgcctcc gcatggagca gagtgccggc gtgcaaagcc
      601 acttcctcga ccccgattac atcggcgtgg acttcatgcc cagcctcgaa ggcagctatg
      661 ccggcgccga cctgatcctg cgcgtgacgc cgccgtcgct ggccgaaatt gccgcgatgc
      721 cggaaggcgc ggtgctgatc ggcttgttga aaccctatga gtcgaaggaa cgactggcag
      781 cactgaacgc caggaaaatc accgccttct cgctcgaact gctgccgcgc atctcgcgcg
      841 cccagagcat ggacgcgctg tccagccagg gcgcctgcgc cggctaccag tgcgggctga
      901 tcgccgccgc ccggtgcacc aagttcttcc cgatgctgac caccgccgcc ggcaccatcc
      961 gcccggcgcg cgtgctcgtc atcggtgccg gcgtcgccgg cctgcaggcg atcgccacct
     1021 gcaagcgcct cggtgccatg gtcgaagcct acgacgtgcg cgccgctgcc aaggagcaga
     1081 tcgaatcgct cggcgccaag ttcgtcgata ccggcgtctc cgccgacggt gccggcggtt
     1141 acgcccgcga gctgaccgcc gaagagaagg cggcgcaggc cgagaagctg gccaaggcgg
     1201 tcgccgccgc cgacgtcgtg atcacgacgg cggcaattcc cggcaagaaa gcgccggtga
     1261 tcgtcacccg cgacatggtc gggcgcatga agtacggcgc catcatcgtc gacatggccg
     1321 ccgagtccgg cggcaactgc gagctgacgc agccgggcga acacgtcgtc gccaacgacg
     1381 tgaacatcca cggcccgctc aacctgccgt cgcgcatgcc gacgcacgcc tccgagctgt
     1441 acgccaagaa catctacaac ttcctctcgc cgtggatcaa ggacggcgcc ctgaacatcg
     1501 actggagcga cgaggtcgtc gccggcaccg tgctgtgccg cgacggcgcc accgtccatg
     1561 ccacggtcaa gcaaatcctg ggagacgcct gatggacggt ctgcttgcgt tgtacatctt
     1621 catgctcgcc gccttcaccg gttacgagat catcgccaag gtaccggtga tcctgcacac
     1681 gccgctgatg tcgggttcca acttcgtcca tggcgtggtg gtggtcggcg cgatgattgc
     1741 gctcggccac gccgagacgc cgctcgaaca ggccatcggc ttcttcgccg tggccctcgg
     1801 cgcggccaac gcggccggcg gctacgtggt caccgaacgc atgctcggca tgttcaagaa
     1861 gaaggaggga acgcaatgac cgcccccttc tacgtccagg gcgcctggtt cgtcggcgcc
     1921 gcgctcttca tcttcggctt gaagggcatg agttcgccgg cgtcggcgcg gcgtggcatc
     1981 gtcatcgccg gctacggcat gctgatcgcc atcgccgcca ctttcctcat ccccggcctg
     2041 cacaacctcg gcctgatcgc cctcgccctg gtactcggcg gcggcgccgc ctggatttcc
     2101 gggcagaagg tgaagatgac cgacatgccg cagatggtcg ccatctacaa cggcatgggc
     2161 ggcggcgcgg cggcggcgat tgccgccatc gagttcgccc gcggcgacgc ccacggtctg
     2221 gtgacgacga cgctcgccgt gctcggcgcg ctgatcggcg ccgtctcctt caccggctcc
     2281 tgcgtcgcct gggccaagct gcagggcgtg ctcaggaagg cttaccgcct gcccgcgcag
     2341 aacgcggtca atgtgacgct ggcggcggtc gccatcggcc tcggcctgag catgatcgcc
     2401 ctggcgccgg cgcagccgca actgatcatc ggcttcttcg tcgtcgccct ggtccttggc
     2461 ctgatcgtca ccctgcccat cggcggcgcc gacatgccgg tggtgatctc gctgttcaac
     2521 gccttcaccg gtctcgccgt cggcttcgag ggctacgtgc tcggcaaccc ggcgctgatc
     2581 atcgccggca tcgtcgtcgg cgccgccggc acgctgctca cccagttgat ggccaaggcg
     2641 atgaaccggc cgctctccaa catcctgttc acgccgatgg tcgcttccgg cccgggcgaa
     2701 gcgatcagcg gctcgatgaa ggaagt
//
LOCUS       DAOSDX010000349         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13111_length_2725_cov_2.977154, whole genome shotgun sequence.
ACCESSION   DAOSDX010000349 DAOSDX010000000
VERSION     DAOSDX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13111_length_2725_cov_2.977154"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLN02_08380"
     CDS             complement(<1..375)
                     /locus_tag="PLN02_08380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOI52379.1"
                     /translation="MSKVYENAAAALDGLLFDGMTLAAGGFGLCGIPENLIGALRESG
                     TRNLTIVGNNAGVDDFGMGPLLKTRQVKKVIASYVGENKEFERQVLAGELDLELVPQG
                     TLAERLRAGGAGIPGFCTRTAAG"
     gene            complement(446..952)
                     /locus_tag="PLN02_08385"
     CDS             complement(446..952)
                     /locus_tag="PLN02_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HOI52380.1"
                     /translation="MSTYTNEQVAHLVEGTLDWETTFRMLSMPKDHGRFEQYLAALQA
                     KVDFPDRIILPLGPHLYIVQQVQTKKWVTQCDCGHVFGDYRENWKLNALVYVRRTEEA
                     MTEVYPKLMAPDTEWQVYREYYCPQCGAMHDVEAPTPWYPVIHDFEPDIEAFYKEWMH
                     LPVPERAD"
     gene            complement(975..>2725)
                     /locus_tag="PLN02_08390"
     CDS             complement(975..>2725)
                     /locus_tag="PLN02_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HOI52381.1"
                     /translation="FGDGYIVTCRKTGANDKPFKDWLHESQRSVRTPKYWILDEKTRI
                     AGCHMIRELVEEVIRADGIEAYEKFAFEVIEEGRRGLMSRIKAMTLPGKYRKVSFVDV
                     PYKHDDVQVSNAFAKLDSIMHSPCEMTIKPDGKWRLDFEGASRWGWHTFNAHQVAFTS
                     GIWVMMCQTLVPTQRINDGAYYATEFRLPKGTWCNPDDRRTGHAYAWHFLVSGWAALW
                     RGLSQSYFSRGYLEEVNAGNANTSNWLQGGGINQDGEIHAVNSFEASSCGTGACAVKD
                     GLNHAAAIWNPEGDMGDIEIWEMAEPLLYLGRNVKANSGGYGKYRGGCGFETLRMVWN
                     AQDWTMFFMGNGFMNSDWGMMGGYPSATGYRFEAHKTGLENRIAIGDSLPLGADLDPS
                     NPDYERHLDATATVKRDKQCITTEDCYANHDLYLNYLRGGPGFGDPIDREPKAIEADL
                     NQKFLLPEYAQKVYGAVFSEDNGVFTVDVAKTAERRTQIRKERLARALPTREWMKAER
                     ERILNKHASTQVKHMFATSFALSEKFTKQFKDFWHLPADWQLLEDELDVPTYGSKHRM
                     DLSLLPDVTTVVQVEE"
BASE COUNT          414 a          841 c          923 g          547 t
ORIGIN      
        1 gccggccgcc gtgcgggtgc agaagccggg gatgccggcg ccgcccgcgc gcaggcgctc
       61 ggccagcgtg ccttgcggca ccagttccag gtccagctcg ccggccagca cctgacgttc
      121 gaattccttg ttctcgccga cataggaggc gatcaccttc ttgacctgcc gcgttttcaa
      181 cagcggcccc atgccgaagt cgtcgacgcc ggcgttgttg ccgacgatgg tcaggttgcg
      241 ggtccccgat tcgcgcagcg cgccgatcag gttctccggg atgccgcaca ggccgaagcc
      301 gccggcggcc agggtcatgc cgtcgaacag cagcccgtcg agggcggcgg cggcattttc
      361 atagactttg ctcattgcgt tgctgctccg aaatcgtcga aaaagccccg ggggcggcca
      421 tcacccgccc ccggggtagc gccgctcagt cggcgcgttc cggcaccggc aggtgcatcc
      481 attccttgta gaaggcttcg atatccggct cgaagtcatg gatcaccggg taccacggag
      541 tcggcgcctc gacgtcgtgc atggcgccgc attgcgggca gtagtactcg cggtacacct
      601 gccactcggt gtccggggcc atcagcttgg gatagacctc ggtcatcgcc tcttcggtgc
      661 ggcgaacgta gaccagggcg ttgagcttcc agttttcgcg gtagtcgccg aagacatggc
      721 cgcagtcgca ctgggtcacc cacttcttgg tctggacctg ctgcacgatg tagaggtgcg
      781 ggccgagcgg caggatgatg cggtccggga aatcgacctt ggcctgcaac gccgccaggt
      841 attgctcgaa gcgaccgtgg tccttgggca tcgacagcat gcggaaggtg gtttcccagt
      901 ccagcgtgcc ctcgacgagg tgggcgacct gttcgttggt gtatgtagac atttctgact
      961 ccttgatacg gtgattactc ttcgacctgg acgacggtgg tgacgtccgg cagcagcgac
     1021 aggtccatgc ggtgcttgga accgtaggtc ggcacgtcca gctcgtcctc gagcagttgc
     1081 cagtcggccg gcagatgcca gaagtccttg aactgcttgg tgaacttctc ggacagcgcg
     1141 aagctggtgg cgaacatgtg cttgacctgg gtcgaggcgt gcttgttgag gatgcgctcg
     1201 cgttccgcct tcatccattc gcgggtcggc agcgcgcggg ccaggcgttc cttgcggatc
     1261 tgggtacgcc gttcggcggt cttcgcgaca tcgacggtga acacgccgtt gtcttcgctg
     1321 aacactgcgc catagacctt ctgggcgtat tccgggagca ggaacttctg gttgaggtcg
     1381 gcctcgatcg ccttcggctc gcggtcgatc ggatcgccga agccggggcc gccgcgcagg
     1441 tagttgaggt agaggtcgtg gttggcgtag cagtcctcgg tggtgatgca ctgcttgtcg
     1501 cgcttgaccg tcgcggtggc gtcgagatgg cgctcgtagt ccgggttgga cgggtcgagg
     1561 tcggcgccca gcggcagcga gtcgccgatg gcgatacggt tttccagccc ggtcttgtgg
     1621 gcttcgaaac ggtagccggt ggccgacggg tagccgccca tcatgcccca gtcgctgttc
     1681 atgaagccgt tgcccatgaa gaacatcgtc cagtcctgag cgttccacac catgcgcagc
     1741 gtctcgaagc cgcagccgcc gcgatacttg ccgtagccgc cggagttggc cttgacgttg
     1801 cggccgaggt aaaggagcgg ctcggccatt tcccagatct cgatgtcgcc catgtcgcct
     1861 tccgggttcc agatcgcggc agcgtggttg aggccgtcct tgacggcgca ggcgccggtg
     1921 ccgcagctcg aagcctcgaa gctgttcacc gcgtggattt cgccatcctg gttgatgccg
     1981 ccaccttgca gccagttcga ggtgttggcg ttgccggcat tgacctcttc caggtagccg
     2041 cggctgaagt aggactggct caagccgcgc cacagcgccg cccagccgga gaccaggaag
     2101 tgccaggcgt aggcgtggcc ggtgcggcgg tcgtccgggt tgcaccaggt gcccttgggc
     2161 aggcggaact cggtggcgta gtaggcgccg tcgttgatcc gttgggtcgg caccagggtc
     2221 tggcacatca tcacccagat gcctgaggtg aaggcgacct ggtgggcgtt gaaggtatgc
     2281 caaccccagc ggctggcgcc ctcgaagtcg agccgccact tgccgtccgg cttgatcgtc
     2341 atctcgcacg gcgagtgcat gatcgagtcg agcttggcga aggcgttgga gacctgcacg
     2401 tcgtcgtgct tgtacggcac atcgacgaag gacaccttgc ggtacttgcc aggcagcgtc
     2461 atcgccttga tccggctcat caggccgcgg cggccttcct cgatgacttc gaaggcgaac
     2521 ttctcgtagg cctcgatgcc gtcggcacgg atcacttcct cgaccagttc gcggatcatg
     2581 tggcagccgg cgatgcgggt cttctcgtcg agaatccagt acttgggcgt gcgcaccgag
     2641 cgctggctct cgtgcagcca gtccttgaac ggcttgtcgt tggcgccggt cttgcggcag
     2701 gtgacgatgt agccgtcgcc gaagc
//
LOCUS       DAOSDX010000350         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13179_length_2713_cov_3.386381, whole genome shotgun sequence.
ACCESSION   DAOSDX010000350 DAOSDX010000000
VERSION     DAOSDX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13179_length_2713_cov_3.386381"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PLN02_08395"
     CDS             complement(<1..1315)
                     /locus_tag="PLN02_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52382.1"
                     /translation="MSIAIRVVLLIALSIAGLIGLGGFSLYQMSSINSGIRHTNEVLL
                     PSILKLEEAQVAFLRARPYLLNVLLEVDPSRRESSVEKFNALIGEMNTAFADYEKLAS
                     TERDRELLARDVKTAEAYAIFARNYMNAAMQGKREEAAEISRNAADTVNGLSNALTEH
                     ARYSQELASANAKEAYDIHAAALRNLIVAIVGISLLVAGVGFLIYRHVAGGLGRLVEV
                     FDQIEKERDFTRQLDVNGKDELARVGHAFNALLGKLRDSFRQISSQADSVNSAAQRVS
                     AAARQMSSASQYQSEAASGMAAAVEEMTVSVNHVADRAEDTRQLASNAGKFAHEGEGI
                     INQTVDSINGIASTVQGASVQLSDLERQSSKISSIVSVIKEIADQTNLLALNAAIEAA
                     RAGEQGRGFAVVADEVRKLAERTGQSTQEIAATIEQMVSGAQAAVA"
     gene            1511..1882
                     /locus_tag="PLN02_08400"
     CDS             1511..1882
                     /locus_tag="PLN02_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52383.1"
                     /translation="MTIPILENVMGNKLAVGLLLAAMGAVPAVAADLKILAFDDNSCA
                     AWKLAADDPDRRAAQVAWARGFLSGHNYANQKQQVTDVSAGTVERNIEQYCRKNPQGL
                     FIDAAYRMSDSMSGRNAPIRK"
     gene            1939..2121
                     /locus_tag="PLN02_08405"
     CDS             1939..2121
                     /locus_tag="PLN02_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52384.1"
                     /translation="MEYQVQDESEHYAMVTLRAKAMSLFFAEKLEAVGLEYRDSQRNN
                     DGTVSVLCRKTEKYKD"
     gene            2271..>2713
                     /locus_tag="PLN02_08410"
     CDS             2271..>2713
                     /locus_tag="PLN02_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOI52385.1"
                     /translation="MRLLLFLFCLIALPGWAASYTPQTVPNPRLVNGSHVADPDQIIG
                     AENLARIDALLRQLEQRNGVQVAVVALDSIGSAEVFDFAQQLFETWGIGHAGRDDGLL
                     VLLVRDQRAIRLHTGYGLEGALPDVVCKRIQREFMVPAFQRGDFGG"
BASE COUNT          437 a          799 c          884 g          593 t
ORIGIN      
        1 tggcgaccgc ggcctgggcg ccggaaacca tctgttcgat cgtcgcggcg atttcctggg
       61 tcgattgacc ggtccgttcg gccagcttgc gcacttcgtc ggccaccacg gcaaagccgc
      121 ggccttgttc gccggcgcgc gccgcttcga tcgccgcgtt gagcgcgagc aggttggtct
      181 ggtcggcgat ttccttgatc accgagacga tgctgctgat cttgctgctt tgccgttcca
      241 ggtctgagag ttgaacggag gcgccttgca ccgtgctggc gatgccgttg atcgagtcca
      301 ccgtctggtt gatgatgcct tcgccttcat gggcgaattt accggcgttg ctggcgagct
      361 gacgggtgtc ttcggcgcgg tcggcaacgt ggttgacgct gaccgtcatt tcctcgaccg
      421 cggcggccat gccggaggct gcctcgctct ggtactggga cgccgaggac atctggcgag
      481 ccgccgccga tacccgctgg gcggcgctgt tgacgctgtc ggcctggctc gagatctggc
      541 ggaagctgtc gcgcagcttg ccgagcaagg cgttgaaggc atggccgacc cgcgccagtt
      601 cgtccttgcc attgacgtcg agctggcggg tgaaatcgcg ttccttctcg atctggtcga
      661 agacctcgac cagacggccg aggccgccgg caacgtggcg atagatcagg aagccgacgc
      721 cggcaaccag caggctgatg ccgacgatgg cgacgatcag gttgcgcagc gccgcggcgt
      781 ggatgtcgta ggcctccttg gcgttggcgc tggccagttc ctggctgtac ctggcgtgct
      841 cggtgagtgc gttggacagg ccgttgacgg tgtccgccgc gttgcgggaa atttccgccg
      901 cttcctcgcg tttgccctgc atggcggcat tcatgtaatt gcgggcgaag atcgcatacg
      961 cctcggcggt tttcacgtca cgcgccagca gttcgcggtc gcgctcggtc gaggccagtt
     1021 tttcgtagtc ggcgaacgcg gtgttcatct cgccgatcag cgcgttgaat ttttccactg
     1081 agctttcccg gcggcttggg tcgacttcca gcaacacatt gagcagatag gggcgcgcac
     1141 gcaggaaggc cacctgggct tcttcgagtt tgaggatgct gggcagcaga acctcgttgg
     1201 tatgccggat gccgctgttg atgcttgaca tctggtacag gctgaatccg ccgaggccga
     1261 tcaggccggc aatggacagg gcgatcagta atacgacgcg aatggcgatg ctcatgtgtt
     1321 ctccgttgtt gagtcgagtg gtttgttgtt gtctattggg gattttactt atttttacgt
     1381 tattgatcga ttttttcaaa attacgatat gtgatatttg tttattgaaa tttcagtttt
     1441 gcttctctat ggtctattgg tttcgagcgc ggatttcccg acatcggcaa gctggtctat
     1501 gctctgcctg gtgactatcc cgatcctgga gaatgtgatg ggtaacaagc tcgcggtggg
     1561 gctgctgctc gccgccatgg gggcagtgcc agcggtggcg gccgatctga agattctcgc
     1621 cttcgacgac aattcctgcg ctgcctggaa gctggcggcc gacgatcccg accggcgtgc
     1681 cgcgcaggtc gcctgggcgc gcggtttcct cagcggtcac aactacgcca accagaagca
     1741 gcaggtcacc gacgtttcgg ccggtaccgt cgagcgcaac atcgagcagt actgccgcaa
     1801 gaatccgcaa ggactgttca tcgacgccgc ctaccggatg agcgacagca tgtccgggcg
     1861 caacgcgccg atccgcaagt agtcagcaat tcccggtctg cggttagaat atgccaattg
     1921 acataaccgg ggattgcaat ggaatatcag gtgcaggacg agtccgaaca ttacgcgatg
     1981 gtgaccctgc gggcgaaggc gatgagcctg ttcttcgccg agaagctgga agcggtcggt
     2041 cttgaatacc gggacagcca gcgcaacaac gacggcacgg tttccgtact gtgccgcaag
     2101 accgaaaaat acaaggactg aacggtggtc ggtggcgccc gccggccagt tttctgaccc
     2161 gctcggcgcc gattgcgcct tgccgctgtc cttgccctgc cgtcccttgc gcgcgggtgg
     2221 tttccggcaa cccgggcggg ggggcgcaac ggtttttccg gtgtctcgca atgcgcctcc
     2281 tgctcttcct gttctgcctg attgcgctgc ccggctgggc cgcgtcctac acgccgcaga
     2341 ccgtgcccaa tccaaggctg gtcaacggca gccatgttgc cgatcccgac cagatcatcg
     2401 gcgccgaaaa cctggcgcgc atcgatgccc tgttgcgcca gctggagcag cgcaacggcg
     2461 tgcaggtggc ggtggtggcg ctcgattcga tcggtagcgc cgaagttttc gatttcgccc
     2521 agcaattgtt cgagacctgg ggcatcggcc acgccgggcg tgacgacggt ttgctcgtgc
     2581 tgctggtgcg cgaccagcgg gcgatccgcc tccataccgg ttacggactc gaaggcgcgt
     2641 tgccggacgt ggtctgcaag cgcatccagc gcgaattcat ggtgcccgcc ttccagcgtg
     2701 gcgatttcgg cgg
//
LOCUS       DAOSDX010000351         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13219_length_2702_cov_3.392898, whole genome shotgun sequence.
ACCESSION   DAOSDX010000351 DAOSDX010000000
VERSION     DAOSDX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13219_length_2702_cov_3.392898"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLN02_08415"
     CDS             <1..364
                     /locus_tag="PLN02_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOI52386.1"
                     /translation="ITSNRPYKAGWDPAESIRRMAEWQGHFDPAVFQAFVRSLGIYPV
                     GSLVRLESGKLGVVVEQGELSLLKPRVKVFFSTRSQAYLKPEIIDIARSREKIAGRED
                     AAKWGIRDIDRWWAGDKR"
     regulatory      386..447
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            523..1938
                     /gene="ahcY"
                     /locus_tag="PLN02_08420"
     CDS             523..1938
                     /gene="ahcY"
                     /locus_tag="PLN02_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOI52387.1"
                     /translation="MAETQDYVIADLSLADWGRKEIRIAETEMPGLMAIREEFAKTQP
                     LKGARITGSLHMTIQTAVLIETLTALGAEVRWASCNIFSTQDHAAAAIAATGVPVFAV
                     KGESLTDYWDYTHRIFEWADGGQGQKVYSNMILDDGGDATLLLHLGARAEQDLSVLDH
                     PDSEEAVVLFASIKAKLKTDPTWYSTRLAQVKGVTEETTTGVHRLYQMFERGELKFPA
                     INVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIGVVLGYGDVGKGCAQSLRGL
                     GATVWVTEIDPICALQAAMEGYRVVTMEDAANMGDIFVTTTGNVGVITHDHMKAMKNN
                     AIVCNIGHFDSEIEVASLRQYTWENIKPQVDHIIFPDGKRIILLAEGRLVNLGCATGH
                     PSFVMSNSFANQTLAQIELFTKTADYPVGVYVLPKHLDEMVARLHLKKIGANLTTLSE
                     AQAKYIGVPVNGPYKPNHYRY"
     gene            1950..2303
                     /locus_tag="PLN02_08425"
     CDS             1950..2303
                     /locus_tag="PLN02_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOI52388.1"
                     /translation="MLRLLAVWIVNALALLALPYLLPSIQVAGLSTALLVAIVLGLIN
                     TLLRPLLILLTLPVTLLTLGLFIFVINALLFQLAGNLVDGFQVGGFWSALFGSLAYSL
                     ISWALSSVLLGKSRR"
     gene            2300..>2702
                     /locus_tag="PLN02_08430"
     CDS             2300..>2702
                     /locus_tag="PLN02_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOI52389.1"
                     /translation="MSMNSFHARQGGRQRDEKAAKPPVERHGLLRADALLVQRGLAAS
                     RTQAQRLIGEGRVFADGKPVGKPSLELPADAALTVQADDSDRYVSRGGLKLAGALAQT
                     GLNAAAKTCLDVGQSTGGFTDCLLQAGAARVV"
BASE COUNT          506 a          931 c          817 g          448 t
ORIGIN      
        1 catcacatcg aaccggccct acaaggcggg ctgggatccg gccgagtcga tccggcgcat
       61 ggccgagtgg cagggccatt tcgacccggc ggtgttccag gccttcgtca ggagcctggg
      121 catctacccg gtcggctcgc tggtccgact ggaatcaggc aagctcggcg tcgtcgtcga
      181 gcagggagaa ctatcgctgc tcaagccgcg ggtcaaagtc ttcttctcga cccgctcgca
      241 ggcctacctc aagccggaga tcatcgacat cgccaggagc cgcgaaaaga tcgccggccg
      301 cgaggatgcg gcaaaatggg gcatccgcga catcgaccgc tggtgggccg gcgacaagcg
      361 ctgaaaacgc gtttataatc cgcaacctac cgaggagcgc tgcaggaggt cactcccagg
      421 ctcggttcaa aacggcgctc actgtccaac cctgccgggc gcagggggcg cagctgagca
      481 tgccgcacct ccctccccgg tcacagttca aggagcttta ctgtggctga aacccaagac
      541 tacgtcattg ccgatctttc ccttgccgac tggggtcgca aggaaatccg catcgccgaa
      601 accgaaatgc cgggcctgat ggccatccgc gaggaattcg cgaaaacgca accgctgaag
      661 ggcgcgcgca tcaccggctc gctgcacatg acgatccaga ccgccgtgct gatcgagacg
      721 ctgaccgcgc tcggcgccga agtccgctgg gcctcgtgca acatcttctc gacccaggac
      781 cacgccgccg ccgccatcgc cgccaccggc gtgccggtgt tcgcggtcaa gggcgaatcg
      841 ctgaccgatt actgggatta cacccaccgc atcttcgaat gggccgacgg cggccagggt
      901 caaaaagtct actcgaacat gatcctcgac gatggcggcg acgccaccct gctgctgcac
      961 ctcggcgccc gcgccgagca ggatctgtcg gtgctcgacc atccggacag cgaggaagcc
     1021 gtggttctct tcgccagcat caaggccaag ctgaagaccg atccgacctg gtattccacc
     1081 cgtctcgccc aggtcaaggg cgtcaccgaa gaaaccacca ccggcgtgca tcgcctgtac
     1141 cagatgttcg aacgcggcga actgaagttc ccggcgatca acgtcaatga ttcggtgacc
     1201 aagtccaagt tcgacaacct ctacggctgc cgcgaatcgc tggtcgacgg catcaagcgc
     1261 gccaccgacg tgatgatcgc cggcaagatc ggcgtggtgc tgggctacgg cgacgtgggc
     1321 aagggctgcg cccagtccct gcgcggcctc ggcgccaccg tctgggttac cgaaatcgac
     1381 ccgatctgcg ccctgcaggc agcgatggaa ggctatcgcg tggtcaccat ggaagacgcc
     1441 gccaacatgg gcgacatctt cgtcaccacc accggcaacg tcggcgtcat tacccatgac
     1501 cacatgaagg cgatgaagaa caatgccatc gtctgcaaca tcggccattt cgactcggaa
     1561 atcgaagtcg caagcctgcg ccagtacacc tgggagaaca tcaagccgca ggtcgaccac
     1621 atcatcttcc cggacggcaa gcgcatcatc ctgctcgccg aaggccgcct ggtgaacctc
     1681 ggctgcgcca ccggccaccc cagcttcgtc atgagcaact cgttcgccaa ccagacgctg
     1741 gcgcagatcg aactgttcac caagaccgcc gactacccgg tcggcgtcta tgtgctgccc
     1801 aagcatctcg acgaaatggt ggcccgcctg cacctgaaga agatcggcgc caacctgacg
     1861 acgctgagcg aggcccaggc caagtacatc ggcgtgccgg tgaacggccc gtacaagccg
     1921 aaccactacc gctactgagt ccgaccaaca tgctccgact gctcgccgtc tggatcgtca
     1981 atgcgctggc cctgctggcg ctgccttatc tgctgccgtc gatccaggtg gcgggtctga
     2041 gcaccgccct gctggtcgcc atcgtgctcg gcctgatcaa cacgctgctg cggccgttgc
     2101 tgatcctgct caccctgccg gtgacgctgc tgacgctggg gctgttcatc ttcgtcatca
     2161 atgcgctgct gttccagcta gccggcaatc tggtcgacgg tttccaggtc ggcggtttct
     2221 ggtcggccct gttcggttcg ctcgcctaca gcctgatttc ctgggctttg agtagcgtgt
     2281 tgttgggcaa atcccggcga tgagcatgaa ctccttccac gcccggcaag gtggccgaca
     2341 gcgtgacgaa aaagcggcca aaccgccggt cgaacggcac ggcctgctgc gcgccgacgc
     2401 cctgctcgtc cagcgcggcc tggcggcgtc gcgcacccag gcgcaacggc tgatcggcga
     2461 gggccgggtg ttcgccgatg gcaagccggt cggcaagccc tcgctggaac taccggccga
     2521 tgcggcgctg accgtgcagg cggacgacag cgatcgctac gtctcgcgcg gcggcctcaa
     2581 gctggccggc gcgctggctc agaccggact caacgccgcc gccaagacct gcctcgacgt
     2641 cggccagtct accggcggct tcaccgactg cctgctgcag gctggcgccg cccgcgtcgt
     2701 cg
//
LOCUS       DAOSDX010000352         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13234_length_2699_cov_3.352874, whole genome shotgun sequence.
ACCESSION   DAOSDX010000352 DAOSDX010000000
VERSION     DAOSDX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13234_length_2699_cov_3.352874"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PLN02_08435"
     CDS             complement(<1..1268)
                     /locus_tag="PLN02_08435"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI52390.1"
                     /translation="MTIHSSVNLPDVADVDRRMAFAFGGLIFVLMTVILLAGGGYLYQ
                     VMSREQDKLSSLTTRVLANAVNRISFSGKYHARLMLEEVKESHPDLVYLRLIDTSGRV
                     IADSDPAHNDAQVDAELQPLIDQVVSGKTPEVVRQLMRDDVPIREVTLIYRGGYDNAV
                     LGVIQVGLSEAESSQALRTGMVFVIVLTSGLLLIGIFLTLRLSSYFAMPVRRVAQELD
                     QERMFLRTVISTIPDLVWLKDGNGVYLSCNREFERFFGKSEAEIVGRTDYDFVDQALA
                     DSFRANDRAAVEAGRPLVNEEWITYASDGHRAFLETTKTPMFAPDGALIGVLGIGHDI
                     TESRANQLEIVRNRDHLEEVVAERTVELKQAKDAAETANMAKSAFLANMSHEIRTPLN
                     AITGMAHVIRRGGVSEKQAEQLDKLEGAGH"
     gene            complement(1280..2272)
                     /locus_tag="PLN02_08440"
     CDS             complement(1280..2272)
                     /locus_tag="PLN02_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOI52391.1"
                     /translation="MSAENAYKVGVLYWSMSIPGQVAMRQGLEAEAKRINAQAARSGG
                     RGVVLLPEVAGDGGDGVERQIRQMRAMVAARPDIIVVQPSDNAALAEPLKAANAAGIP
                     VVAYDQYISGGKLASYVTSDNRQAGYLGGEYIAARFPGNTPLRLVLVEYPLVSSTVER
                     LNAFLDALRDHGKAFRILKTYQAVEPVGGRKAGEAILRDFPQRDSVDVVFTVNDGGGL
                     AVVDALAKAGRNEIAVASVDGDPVAVGNIRAGRLTVIDAAQFCGPLGAEAMRVAYAVL
                     RKQPVARHVLVPVFPVTRETVDRYPGWQGPLPQAFEKPWPANQRSWSGKIVEPR"
BASE COUNT          516 a          909 c          792 g          482 t
ORIGIN      
        1 tggtgccccg ccccctccag cttgtccagt tgctcggcct gcttctcgct gaccccgcca
       61 cgccggatga cgtgcgccat accagtgatg gcgttgagcg gcgtacggat ttcatggctc
      121 atgttggcca ggaaggcgct ctttgccatg ttggccgttt cggctgcgtc cttggcctgt
      181 ttcagttcga cagtacgctc ggcgacgact tcttccaggt gatcgcggtt gcgcacgatc
      241 tccagctggt tggcccggct ttccgtgatg tcgtggccga tgccgagcac cccgatgagc
      301 gccccgtcgg gcgcgaacat cggtgtcttg gtcgtttcga ggaaggcccg atggccgtcc
      361 gaggcgtagg tgatccactc ctcgttgacc aacggtcggc cggcctcgac ggcagcccgg
      421 tcattggccc ggaagctgtc ggccaatgcc tgatcgacga aatcgtaatc ggtccgcccg
      481 acgatctccg cttccgactt gccaaagaaa cgttcgaact cgcggttgca ggaaaggtag
      541 accccgttcc catccttcag ccagaccaga tcgggaatgg tcgaaatgac ggtacgcaag
      601 aacatgcgct cctggtcgag ctcctgggca acgcgccgga ccggcatggc gaagtacgaa
      661 ctcagacgca aggtcaggaa gataccgatc agcagcaggc ccgaggtcag cacgatgacg
      721 aacaccatgc cggtgcgcag ggcctgcgaa ctctcggctt ccgacaggcc gacctggatg
      781 acgccaagca cggcattgtc gtagccaccg cggtagatca gcgtcacttc gcggatcggc
      841 acatcatcgc gcatcagttg ccgcaccact tccggcgttt ttcccgagac cacctggtcg
      901 atcagcggct gcagttcggc atcgacctgg gcgtcgttgt gcgccggatc gctatcggca
      961 atcacccggc cactggtgtc gatcaagcgc agatagacga gatccgggtg cgattccttg
     1021 acctcttcca gcatcaggcg ggcgtggtac ttgccggaaa agctgatgcg attgaccgcg
     1081 ttggccagca cccgcgtggt cagcgacgac aacttgtcct gctcgcggct catgacctga
     1141 tacagataac cgccaccggc aagcagaatg acggtcatca aaacgaagat caaccctccg
     1201 aaggcgaacg ccatgcggcg gtctacatcg gcgacatcgg gcaaattgac gctggagtga
     1261 attgtcacga tacggcctgt tatctcggtt cgacgatctt gcccgaccac gaccgctgat
     1321 tggcaggcca gggcttttca aaggcctggg gcagcggccc ctgccaaccg ggatagcggt
     1381 ccaccgtttc acgggtgacc ggaaaaaccg ggaccaacac atgccgggcg accggctgct
     1441 tgcgcagtac ggcataggcg acacgcatcg cttcggcgcc gagcgggccg caaaactggg
     1501 cggcatcgat gacggtcagc cgcccggccc ggatgttgcc gacggcgacc ggatcgccat
     1561 cgacgctggc caccgcgatc tcgtttcgcc cagccttggc cagcgcatcg acgacggcca
     1621 gcccgcctcc atcgttcacc gtaaacacca catcgacgct gtcgcgctgt ggaaaatcgc
     1681 gcaggatcgc ctcgcccgcc ttgcgtccac cgaccggctc gaccgcctgg taagtcttga
     1741 gaatcctgaa ggccttgcca tgatcgcgca gggcatcgag gaaagcgttc aggcgttcga
     1801 ctgtggacga gaccagtgga tactcgacca gcaccagccg caagggcgta ttgccgggaa
     1861 agcgggcggc gatgtactcg ccgcccagat agccggcctg ccggttgtcc gaggtaacgt
     1921 aggaggccag cttgccgccg ctgatgtact ggtcgtaggc cacgacggga atcccggcgg
     1981 cgttggccgc cttcagcggc tcggccagcg ccgcattgtc ggacggctgg acaacgatga
     2041 tgtccggtct ggccgccacc atggcccgca tctggcgaat ctgacgctcg accccatcgc
     2101 cgccgtcgcc cgccacctcc ggcagcagga caacgccccg cccgccgctg cgcgccgcct
     2161 gcgcgttgat cctcttggct tccgcctcca gcccctggcg catcgccacc tggccgggaa
     2221 tgctcatcga ccagtaaagc accccaacct tataggcatt ttcggcgctc accgtgccgc
     2281 acagacacaa cactagaccc caaagtaccc accgcaagcg atgtcgaacc tgcatgaaat
     2341 atccctgaga cagtcctctt cggaaaccgc atggcgctcg gccccgtcac ccgctcaaca
     2401 acggtgacgt ccccgtttcc ccggacgacg aaggcgcagt ataaatcctt acttccagca
     2461 ttgcttatcc ggcttgccta aattgcctaa cgctgacgtc cggccttgct tcggccaatc
     2521 atgagacagg cccaagcgaa atccggagta ggcacgaagc agttcgtcgc atgcgacacc
     2581 aagacaatct gcataacccg agcaccgccc ggagtcggtc gaccgggcgc gcccgccgat
     2641 gatagcgtgg taagctccac tgcctacgga gtcttccaac ccatgtccct tcttgccag
//
LOCUS       DAOSDX010000353         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13242_length_2697_cov_4.590462, whole genome shotgun sequence.
ACCESSION   DAOSDX010000353 DAOSDX010000000
VERSION     DAOSDX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13242_length_2697_cov_4.590462"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLN02_08445"
     CDS             complement(<1..1233)
                     /locus_tag="PLN02_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOI52392.1"
                     /translation="MKLTVAKKMALLGGSALLGIALLTGLGQQQMGKVYEAASYCSVN
                     TVPSMTTLNTALAEFSRIRVGTYRHVLNTDDKAMVEIESKLKDATAKFDKALNDYESL
                     VSGDKDKALLEADKKIRDEYYLGLDQTLELSRANKTEQAREVMAKNAALAEKLNDALD
                     EHMQYNVDLGKKGTDEAAATKGSALTLSLLIAVVTLVAVGFIAFVVTRAFLKQLGGEP
                     DFAADVANKIAAGDLTSKIEIKSGDSTSLLCAMKTMQDSLTGIVGEIKELVVAANRGD
                     FNTKMNLAGKAGYSKDLADLLNQLSATVDTAFKDVVRVSSALAAGDLSQKVNREYQGD
                     FDQAKQAVNTTVDALTRIVGEIREIVGAANRGDFSIKMELAGKAGYTKELSDLLNQLS
                     STVDTAFKDTIYVAQALEQ"
     gene            complement(1265..>2697)
                     /locus_tag="PLN02_08450"
     CDS             complement(1265..>2697)
                     /locus_tag="PLN02_08450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOI52393.1"
                     /translation="TQAELEEALVLQATIPVETTAPPLGEVLVQQKLVQPEVVDAALD
                     KQKQLREKHSGEAALIRVNAEKLDQHINLIGELIIASAGINLAAARSGQPELQEAASL
                     LNRLVEDIRDSALQLRMVQIGATFNKFQRVVRDVSKEIGKDIRLEIAGAETELDKTVV
                     ERIGDPLTHLVRNSMDHGIESAELRRARGKPGHGTLKLNAYHDSGSIVIEVADDGGGL
                     PRDKILAKAIERGLVKPEQVLSDSEIHALIFEPGFSTAEQVNNLSGRGVGMDVVRRNI
                     VALRGSIEIESSEGVGTTIRIRLPLTLAIIDGFLVGVGDSSFVVPLEMVVECVELSAA
                     EIEAAQGRNQLDLRGEVLPFIRLRDLFEIPQAAAPEIGNAQEILTQDVDEELAFLLEP
                     VRRENVVVVDYAGRRAGLVVDMLQGEYQTVIRPLGAVFNGLQGISGFTILGSGQVALI
                     LDVPGLVRRVAAHEGLRKQLSLSEAC"
BASE COUNT          478 a          828 c          791 g          600 t
ORIGIN      
        1 ctgctcgagc gcctgggcaa cgtagatggt gtccttgaac gcggtatcga ccgtgctgga
       61 aagctggttc agcaaatccg acaattcctt cgtataaccc gccttgccgg caagctccat
      121 cttgatcgag aagtcaccgc ggttggctgc accgacgatc tcccgaatct cgccaacgat
      181 gcgggtcagt gcgtccaccg tcgtattcac cgcctgcttg gcctgatcga agtcaccttg
      241 gtactcccgg ttcaccttct gtgacaggtc gcccgccgcc agcgccgaag aaacccggac
      301 cacatccttg aacgccgtat cgaccgtggc cgacaactga ttcagcaaat cggcaagatc
      361 cttgctgtag cccgctttcc ccgccagatt catcttcgtg ttgaaatcgc cgcggttcgc
      421 cgcaacaacc agctccttga tttccccgac gattcccgtc agcgaatcct gcattgtctt
      481 catcgcacac agcaggctgg tactgtctcc ggacttgatc tcgatcttgc tcgtgaggtc
      541 gccggcggca atcttgttgg ccacatcagc ggcgaagtcg ggttcgcccc ccagttgctt
      601 gaggaatgcc cgggtaacga caaaggcgat gaagccgacg gcaacgagag tcactaccgc
      661 aataagcagc gaaagtgtca aagcgctccc tttggttgca gctgcttcat ccgtgccttt
      721 cttccctaga tcgacgttgt attgcatatg ctcgtcaaga gcatcgttga gcttttcggc
      781 aagagcggca ttctttgcca ttacctctct cgcttgctcg gtcttgtttg cacgggataa
      841 ttcgagcgtc tgatccaagc cgaggtaata ctcgtctctg atctttttat ctgcctccag
      901 cagggctttg tccttatcgc cagagaccaa ggattcgtag tcattcaacg ccttgtcgaa
      961 tttcgccgtc gcgtccttta gcttcgattc gatttcgacc attgccttgt catcggtatt
     1021 gagcacgtgg cgataggtcc cgactcgaat tcggctgaat tcagccaggg ctgtattcag
     1081 cgttgtcata ctgggaacgg tgtttaccga gcaatagctt gctgcttcgt agactttacc
     1141 catttgctgc tgccccagtc ctgtcagcaa cgcgatgccg agtaaagcgg aaccgccgag
     1201 cagggccatt ttttttgcaa cggtgagttt catcgttatc tccgggtgga aaataggttt
     1261 gggttcagca ggcttcactg agggaaagtt gcttgcgtag gccttcgtgt gcggcgacac
     1321 ggcggaccag tcctggcaca tcgaggatca gggcgacctg gccgctgccc aggatggtga
     1381 agccgctgat tccctgcagg ccgttgaaga cagcgccgag cggacggatc accgtctggt
     1441 attcgccctg cagcatgtcg acgacgaggc cggcccggcg accggcgtaa tcgaccacca
     1501 ccacgttttc ccgacgtacc ggttcaagga ggaaggcgag ctcctcgtcc acgtcctggg
     1561 tgaggatttc ctgtgcattg ccgatttccg gcgccgccgc ttgcggaatc tcgaaaaggt
     1621 cgcgcagacg gatgaagggc agcacttcgc cgcgcaggtc gagctggttg cgcccttgcg
     1681 ccgcctcgat ttcggcggcg ctgagctcga cgcactcgac gaccatctcc agcggcacga
     1741 cgaaggagga atcgccgaca ccgaccagga agccgtcgat gatggccagg gtcagcggca
     1801 ggcggatgcg gatggtggta ccgacgcctt cgctgctttc gatctcgatg ctgccgcgca
     1861 gggcgacgat gttgcggcgc acgacatcca tgccgacgcc gcggccagat aggttgttga
     1921 cctgctcggc ggtggagaag ccgggctcga agatcagcgc atggatctcg ctgtcgctga
     1981 gaacctgctc aggcttgacc agaccgcgct cgatcgcctt ggccaggatc ttgtcccggg
     2041 gcagaccgcc gccatcgtcg gcaacctcga tgacgatgct gccggaatcg tggtaggcgt
     2101 tcagcttcaa tgtgccgtgc ccgggcttgc cgcgcgcccg ccgcagttcc gcggactcga
     2161 tgccgtgatc catggaattg cgtaccagat gggtcagcgg atcacctatc ctctcgacca
     2221 ccgtcttgtc gagttcggtc tcggcaccgg cgatttccag ccggatatcc ttgccgatct
     2281 ccttcgagac gtcgcgcacc acccgctgga acttgttgaa ggttgcgccg atctggacca
     2341 tgcgcagttg cagcgcggag tcgcggatgt cctcgacgag ccggttgagc aaggaggcgg
     2401 cctcctgcag ttcgggctgg ccactgcgtg cggcggccag gttgatgccg gcactggcga
     2461 tgatcagttc gccgatcagg ttgatgtgct gatcgagttt ttccgcgttg acgcggatca
     2521 acgcggcctc cccggaatgc ttctcgcgga gctgtttctg cttgtcgagc gcggcatcga
     2581 ccacttccgg ctgcaccagc ttctgctgca ccagcacctc gcccagcggt ggcgccgtgg
     2641 tctcgacggg aatcgttgcc tgcaggacga gcgcctcttc cagttcggcc tgggtca
//
LOCUS       DAOSDX010000354         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13261_length_2695_cov_2.947348, whole genome shotgun sequence.
ACCESSION   DAOSDX010000354 DAOSDX010000000
VERSION     DAOSDX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13261_length_2695_cov_2.947348"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLN02_08455"
     CDS             complement(<1..519)
                     /locus_tag="PLN02_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI52394.1"
                     /translation="MTLTELRYIVALAQEKNFSRAAERCSVSQPTLSVAIARLEDELG
                     VQLFERGKGFVSPSLVGSKVVEQAQLALGEAEKVRQIAQSGRDQLDGHLRLGIIHTIG
                     PYLLPQLIHSLKAIAPNMPLAIEENMTANLADMLRDNDIDVAIIALPFDLPGVLTRPL
                     YDERFKVIVPRGH"
     gene            682..1680
                     /locus_tag="PLN02_08460"
     CDS             682..1680
                     /locus_tag="PLN02_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HOI52395.1"
                     /translation="MKRTVLSAAILLLSGMALQGAAQAASDEPIQPIKPPASINVGMV
                     ELGKKLYFDPRLSKSGIMSCNTCHNLSMGGTDNIPTSVGDHWQQGPINAPTVLNSSLN
                     LAQFWDGRAADLKAQAGGPIANPGEMAFTHTLAVNVLQSIPGYVREFKMVFGKDKIDI
                     DQVTLAIAEFEKTLVTPNSRFDQWLLGKKDALSKDELEGYALFKDSGCVACHNGEAVG
                     GNSFQKMGIVEPYKGKMSDGRAAVTGKDEDRFNFKVPTLRNVEMTYPYFHDGAANTLT
                     EAVDVMGRLQLGKKFTKDENAKVVAFLKALTGDQPNFTLPILPPSSDTTPRPTPFK"
     gene            1805..2419
                     /gene="ccmA"
                     /locus_tag="PLN02_08465"
     CDS             1805..2419
                     /gene="ccmA"
                     /locus_tag="PLN02_08465"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HOI52396.1"
                     /translation="MLEADNLECVRGERRLFAGLSFKLEAGELLYLQGRNGAGKTSLL
                     RMLIGLLPPEAGDIRWQGETIRRRRDDYHAALCYLGHLNAIKEELTPLENLLAGARLA
                     GETLSEDDAIDALEQVGLAGREDLACKYLSQGQKRRVALARLVIDRRPLWILDEPFVA
                     LDVGAVDWLAGVISAHLQRGGMAVMTTHQPVAIPAGTVRERRIG"
     gene            2429..>2695
                     /locus_tag="PLN02_08470"
     CDS             2429..>2695
                     /locus_tag="PLN02_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOI52397.1"
                     /translation="MFDIVFTVIVRDLKLAMRRQADIVSALFFFIIVVSLFPLGIGPE
                     LDLLRQLAPGVLWVAALLATMLSLPRLFADDLRDGTLEQLALSPH"
BASE COUNT          515 a          854 c          812 g          514 t
ORIGIN      
        1 atggccgcgc ggcacgatga ccttgaaacg ctcgtcgtag agggggcggg tcagcacccc
       61 gggaaggtcg aaaggcagcg cgatgatggc gacatcgatg tcgttgtcgc gcagcatgtc
      121 agccaggttg gccgtcatgt tctcttcgat cgccagcggc atgttcggcg cgatcgcctt
      181 cagcgaatgg atcaattgcg gcagcaggta ggggcctatc gtatggatga tccccaggcg
      241 caggtggccg tcgagctgat cgcggccgct ctgggcaatc tgtcgcacct tctctgcctc
      301 accgagagcc agttgcgcct gttcgaccac cttgctgccg accaggctgg ggctgacgaa
      361 gcccttgccg cgctcgaaaa gttgcacgcc aagctcatcc tcgaggcggg caatggccac
      421 gctcagggtc ggctggctga cggaacagcg ctcggcggca cggctgaaat tcttttcctg
      481 ggcaagcgcc acgatgtaac gcagttcggt cagggtcatg ccggtttcct cctaagactg
      541 aggatcatac cctcatgatt tataggggat acctatggtt actataggga aatcccataa
      601 aaaccattga tttggatcaa atttgcaaat ttctcaaggg tgagaatggt ccggcaaatt
      661 tcacaacgag ggagatcaat aatgaaacgt accgtccttt ccgccgccat cctcctgctt
      721 tccggcatgg ccctgcaggg tgccgcgcag gccgccagcg acgaaccgat ccagccgatc
      781 aagccgcccg ccagcatcaa tgtcggcatg gtcgaactgg gcaagaaact gtacttcgac
      841 ccgcgcctgt ccaaatccgg catcatgtcc tgcaacacct gccacaacct gtcgatgggc
      901 gggaccgaca acatcccgac ctcggttggt gaccactggc agcaaggccc gatcaacgca
      961 ccgacggtgc tcaactccag cctcaacctg gcccagttct gggacggccg cgccgccgac
     1021 ctgaaggcgc aggctggcgg tccgatcgcc aacccggggg aaatggcttt cacgcacacc
     1081 ctggcggtca acgtgctgca gtcgattccg ggctatgtcc gcgaattcaa gatggtcttc
     1141 ggcaaggaca agatcgatat cgaccaggtg acgctggcca ttgccgaatt cgagaagacc
     1201 ctggtgacgc cgaactcgcg cttcgaccag tggctgcttg gcaagaagga tgcgctgagc
     1261 aaggacgagc tggaaggcta cgcgctgttc aaggacagcg gctgcgtcgc ctgtcacaat
     1321 ggcgaagctg ttggcggcaa ttccttccag aagatgggca tcgtcgagcc gtacaagggc
     1381 aagatgtcgg atggtcgcgc cgccgtcacc ggcaaggatg aagatcgctt caacttcaag
     1441 gtgccgacct tgcgcaacgt cgaaatgacc tatccgtact tccatgacgg tgccgccaac
     1501 acgctgaccg aggcggtcga cgtgatgggc cgcttgcaac taggcaagaa gttcaccaag
     1561 gatgaaaacg ccaaggtcgt tgccttcctg aaggccctga ccggcgacca gccgaacttc
     1621 acgctgccga tcctgccgcc gtcctccgac acgacaccgc gtccgacgcc gttcaaataa
     1681 gcgctaatac cgcctctcga tttccggggg ctgcggcccc cgtttttttc gatttgattc
     1741 accgcaaact gccggtacac tggcgcgtcc ataatacgtg ccggataaat cccgggccct
     1801 gaccatgctg gaagccgaca atctggaatg cgtgcgcggc gagcgccgcc tgttcgcagg
     1861 cctgagcttc aagctggaag ccggagaatt gctctatctg cagggccgta acggtgccgg
     1921 caagaccagc ctcttgcgca tgttgatcgg cctgctgcca ccagaggccg gcgacatccg
     1981 ctggcagggc gaaacgatcc gccgccgccg cgacgactac cacgccgctc tctgctatct
     2041 cggtcacctc aacgcgatca aggaagaact gacgccgctc gagaacctgc tggctggcgc
     2101 gcgcctggcc ggcgaaacct tgtccgagga cgatgcgatc gatgctctgg aacaggtcgg
     2161 cctcgccggc cgcgaggatc tcgcctgcaa gtatttgtcg cagggacaga agcggcgcgt
     2221 cgcgctggct cgcctggtga tcgaccggcg gccgctgtgg attctcgacg aacctttcgt
     2281 cgctctcgac gtcggcgcgg tcgactggct agccggtgtg atctcggcgc acctgcagcg
     2341 cggcggcatg gcggtgatga cgacgcatca gccggtggcg attcccgccg gcacggtgcg
     2401 cgaacggcgc atcggctgag gctcatcgat gttcgatatc gtcttcaccg tgatcgtccg
     2461 cgacctgaaa ctggccatgc gccgccaggc cgacatcgtc tccgcgctct ttttcttcat
     2521 catcgtcgtc agcctctttc cgctcggcat cggtccggaa ctcgatctgc tgcgccagct
     2581 ggcgcccggc gtgctgtggg ttgccgctct gctggcgacg atgctgtcgt tgccccgtct
     2641 tttcgccgat gacttgcgcg acggcacgct ggagcagtta gccctgtcgc cacac
//
LOCUS       DAOSDX010000355         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13265_length_2694_cov_2.896173, whole genome shotgun sequence.
ACCESSION   DAOSDX010000355 DAOSDX010000000
VERSION     DAOSDX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13265_length_2694_cov_2.896173"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="PLN02_08475"
     CDS             complement(<1..1833)
                     /locus_tag="PLN02_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI52398.1"
                     /translation="MPAPALSLPAGDKTGYDAAMPGSSPSLSRVWLLTAAAAASCLLV
                     GLIAYRLLLGSYLAHEQQVASQRLDAFALSLEATLNRHESLPGLLALEPSLAALLREP
                     DNAQRIAAANAYLEAAQQGAAVAATYLIDADGLTLAASNWRQPRSFVGHNYAFRPYFT
                     DAVAQGLGRFYGVGVTTGEPGYFLAAPVRSAGRVLGVVVLKVGLEAMEQAMAESGDTL
                     LLADADGIVFLSSQRRLRYRTLAPLPGAISARLAETRQYGNQTIQPLADRPLALDGST
                     PQRLALAGEPIRELLLLSRPVGNLGWRVVQLGHPDEARSAAFSGAMAAALAAAFGLGV
                     AAHLRHRRRRREELRRIHGELEQRIAERTADLTGKVAELQRTEAILHETRDAAVQAGK
                     LAVLGQLSAGISHELNQPLAALRTFADNASALLARGRHEEVAENLQMIGELVGRTGRI
                     VRQLKTFARKEAATPQAVTVTSAIEHALLLVEPRRRDSAATIAVSQDDPALCVVAEAG
                     RFEQVLVNLMLNGLDAMHGQPAPRLEIDVARRGANVRLAVRDHGPGIADDVRNHLFEP
                     FYTTKPAGEGLGLGLAISLAIVESYGGTLRTENAADGGAVFVLTL"
     gene            complement(1815..2195)
                     /locus_tag="PLN02_08480"
     CDS             complement(1815..2195)
                     /locus_tag="PLN02_08480"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HOI52399.1"
                     /translation="MNAICLVAGLLVAPLGEAVTLQWTHSVQKTLWAEDYRLQGDALR
                     LTSARVLGTGAGMEPPPDAVYRDGAWHYTPALPALPKVTLRHSPYVAPYTVCVGADCR
                     PLPAWLPGLPGDTTVDLQPCPPQP"
     gene            complement(2192..>2694)
                     /locus_tag="PLN02_08485"
     CDS             complement(2192..>2694)
                     /locus_tag="PLN02_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOI52400.1"
                     /translation="NYIITSSIAAPALLDLGVPLIVSHMFVFYFGIMADLTPPVALAA
                     FAASSVAKEPAMKIGVKAVQIAIAGFVVPFMAVYDNALMLQGDPSVIAVVYIVGKALL
                     AITLWGAASIGYLRAPLAAWERVWAAAAAFLLVAALPLTDEIGFAACAAFIGWHLLHS
                     RRLQAA"
BASE COUNT          399 a          879 c         1034 g          382 t
ORIGIN      
        1 cagcgtcagc acgaacacgg cgccgccgtc ggccgcgttc tcggtgcgta gtgtgccacc
       61 gtagctctcg acgatggcga gcgagatggc cagcccgagg ccgaggcctt cgccggccgg
      121 cttggtggtg tagaagggct cgaacaggtg gttgcgcacg tcgtcggcga tgcccggccc
      181 atggtcgcgt accgccaggc ggacgttggc gccgcgccgg gcgacgtcga tttcgagccg
      241 cggcgccggt tgtccgtgca tggcgtcgag accgttgagc atcagattga ccagcacctg
      301 ctcgaagcgc ccggcttcgg caacgacgca cagcgccgga tcgtcctggc tgacggcgat
      361 ggtggcggcg ctgtcgcggc ggcgcggttc gaccagcagc agcgcgtgtt cgatcgcact
      421 ggtcacggtg accgcctgcg gcgtcgccgc ctccttgcgc gcgaaggtct tcaactggcg
      481 aacgatgcgc ccggtgcggc cgaccagttc gccgatcatc tgcaggtttt cggctacctc
      541 ttcgtggcga ccgcgggcga gcagggcgct ggcgttgtcg gcgaaggtgc gtagcgccgc
      601 cagcggctgg ttcagttcgt ggctgatgcc ggccgacaac tggccgagga cggcgagttt
      661 gccggcctgc accgcggcgt cgcgcgtttc gtgcaggatc gcctcggtgc gttgcagctc
      721 ggcgactttc ccggtcaggt cggcggtgcg ttcggcgatc cgctgttcca gttcgccgtg
      781 gatgcgccgc agttcctcgc gccgccgccg ccggtggcgc aggtgggcgg cgacgccgag
      841 gccgaaagcg gcagccagcg ccgccgccat cgccccggaa aaggcggcgc tgcgggcttc
      901 gtccggatgc ccgagctgga cgacgcgcca gccgagattg ccgaccgggc ggctcaacag
      961 cagcagttcg cggatcggtt cgccggccag ggccaggcgc tgcggcgtcg agccatcgag
     1021 cgccagcggg cggtcggcca gcggctggat cgtctggttg ccgtattgcc gggtttccgc
     1081 caggcgggcg ctgatcgcgc ccggcagcgg ggccagcgtg cgatagcgca ggcgccgttg
     1141 cgaggagagg aagacgatgc cgtcggcgtc ggccagcagc agcgtgtcgc cggactcggc
     1201 catggcctgc tccatcgcct ccaggccgac cttgaggacg acgacgccga gcacgcggcc
     1261 ggcgctgcgt accggcgcgg cgaggaaata gcccggttcg ccggtggtca cgccgacgcc
     1321 gtagaagcgc cccaggccct gcgccacggc atcggtgaaa tagggccgga aggcgtagtt
     1381 gtggccgacg aaactgcgcg gttggcgcca gttgctggcg gccagcgtca ggccgtcggc
     1441 gtcgatcagg taggtggcgg cgaccgccgc gccctgctgg gccgcttcca ggtaggcgtt
     1501 ggcggcggcg atccgttgcg cgttgtccgg ctcgcgcagc agcgcggcga gcgacggttc
     1561 gagggcgagc aggccgggta gcgattcgtg ccggttgagc gtcgcttcca gcgacagcgc
     1621 gaaagcgtcg aggcgctggc tggccacctg ctgttcgtgc gccagatagc tgccgagcag
     1681 cagacggtag gcgatcaggc cgaccagcag gcagcttgcc gccgccgccg cggtgagcag
     1741 ccagacgcgg gagagggacg gcgacgatcc gggcatggcc gcatcgtaac cagttttatc
     1801 gccggccggc aggctcaggg ctggggcggg catggctgca ggtcgaccgt cgtgtctccg
     1861 ggcaggccgg gcagccaggc cggcagcggc cggcagtcgg cgccgacgca gacggtataa
     1921 ggggcgacat agggcgaatg gcgcagcgtg actttgggca gcgccggcag ggccggggtg
     1981 tagtgccagg cgccgtcacg gtagacggcg tcgggcggcg gctccatgcc ggcgccggtg
     2041 ccaaggacgc gggcagaggt cagacgaagc gcgtcgccct gcaggcggta gtcttccgcc
     2101 cacagggttt tctggaccga atgcgtccat tgcagcgtca ccgcctcgcc gagcggcgcc
     2161 acgaggaggc cggcgacgag gcagatcgcg ttcatgccgc ctgcaggcga cggctgtgca
     2221 gcaggtgcca gccgatgaag gcggcgcagg cggcaaagcc gatctcgtcg gtgagcggta
     2281 gcgccgccac cagcaggaag gcggcagccg ccgcccagac ccgttcccag gcggccagcg
     2341 gggcgcgcag gtagccgatg ctggcggcgc cccagagcgt gatcgccagc aaggccttgc
     2401 cgacgatata gacgacggcg atcaccgacg ggtcgccctg cagcatcagc gcgttgtcgt
     2461 agaccgccat gaacggcacg acgaagccgg cgatggcaat ctgcaccgcc ttgacgccga
     2521 tcttcatcgc cggctccttg gccaccgaac tggcggcgaa ggcggcgagc gccaccggcg
     2581 gcgtcaggtc ggccatgatg ccgaagtaaa agacgaacat gtggctgacg atcagcggca
     2641 cgccgagatc gagcagcgcc ggggcggcga tcgaactggt gatgatgtag ttgg
//
LOCUS       DAOSDX010000356         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13268_length_2693_cov_2.504170, whole genome shotgun sequence.
ACCESSION   DAOSDX010000356 DAOSDX010000000
VERSION     DAOSDX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13268_length_2693_cov_2.504170"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLN02_08490"
     CDS             complement(<1..570)
                     /locus_tag="PLN02_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HOI52401.1"
                     /translation="MATALVAYHADCVDGFGAAWSAWRALGEAALYRPLHHGEHLAGE
                     EIAGRDVYILDFSFAPAELEAMTELAHSVTQIDHHATAHGDWCGHLQDSGDGREIYCD
                     PQRSLTVIFALDRAGAMLAWAQFHHTTPPLLLQHVEDLDLWRFALPGTRNFCRALRLQ
                     PFCFTIWERLAEELASPEAPAYLAFVERGA"
     gene            complement(572..772)
                     /locus_tag="PLN02_08495"
     CDS             complement(572..772)
                     /locus_tag="PLN02_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOI52402.1"
                     /translation="MESRITELEIKISFNEDQIEELNKIIYQQQRQIELLAAELRNLR
                     DQVNSARPQEGRSLRDEIPPHY"
     gene            1012..1088
                     /locus_tag="PLN02_08500"
     tRNA            1012..1088
                     /locus_tag="PLN02_08500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1046..1048,aa:Val,seq:gac)
     gene            1223..>2693
                     /gene="thrS"
                     /locus_tag="PLN02_08505"
     CDS             1223..>2693
                     /gene="thrS"
                     /locus_tag="PLN02_08505"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOI52403.1"
                     /translation="MPDIKLPDGSIRSFAQPVTIAEVAASIGAGLARAALAGKVNGKL
                     VDTSYLIEENIDLAIVTDRDADGLELIRHSTAHLLAYAVKELFPEAQVTIGPVVENGF
                     YYDFAYKRPFTPEDLVAIEKRMAELAKRDIPVSREVWNRDDAVNFFLGQGEKYKAELI
                     AAIPADQQVSLYREGDFVDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDHRNEQLQRIY
                     GTAWAKKEDLDAYLHMLEEAEKRDHRRLGKQFDLFHMQDEAPGLVFWHPKGWSVWQEV
                     EGYMRKVYRDNGYQEVRCPQILDRSLWEKSGHWDHYKDNMFTTSSENRDYAVKPMNCP
                     GHIQVFNSDLRSYRELPLRYGEFGSCHRNEPTGALHGLMRVRGFVQDDGHIFCTEDQI
                     ESEVTAFNALVKKVYADFGFNDVAVKLALRPEGRVGSDEVWDKAEDALRQGLRASGLE
                     WTELPGEGAFYGPKIEFHIKDAIGRSWQCGTMQVDFSMPG"
BASE COUNT          528 a          775 c          838 g          552 t
ORIGIN      
        1 cgcgccgcgt tcaacgaaag caagataggc gggggcttct ggcgatgcga gttcctcggc
       61 caggcgctcc cagatcgtga aacagaacgg ctgcaggcgc agcgcccggc aaaaatttcg
      121 ggtgccgggc agggcaaagc gccacagatc gagatcctcg acgtgctgga gcagcaaagg
      181 cggggtggtg tgatgaaact gcgcccaggc aagcatggcg ccggcgcggt cgagggcgaa
      241 aatcacggtc agcgaacgtt gcgggtcgca atagatttcg cggccgtccc cgctgtcttg
      301 caaatgcccg caccagtcgc cgtgggcggt ggcgtgatga tcgatctggg taaccgagtg
      361 agccagttcg gtcatggctt ccagttcggc gggggcgaac gagaaatcga ggatgtatac
      421 gtcgcgcccg gcaatctctt cgccggcaag gtgttccccg tggtgcagcg ggcggtagag
      481 tgccgcctcg ccaagtgcgc gccatgcact ccaggcggcg ccgaaaccgt ccacgcaatc
      541 ggcgtggtaa gcaacgagcg ctgttgccat ttcagtaatg cggcggaatt tcgtcgcgca
      601 ggctacgtcc ttcctggggg cgtgcgctat tgacctggtc gcgcagattg cgcaactcgg
      661 cggcgagcaa ctcgatctgg cgttgctgct gatagatgat cttgttcaat tcctcgatct
      721 ggtcttcatt gaagctgatc ttgatttcca gttcggtgat ccgggattcc atgcggtgtt
      781 ccatcattct ttgggttgtg cttcccggcg tcttcgcgca gagagcgccg gcgctcgtga
      841 agcaagggta gtgtggcgcc gatcaaggtc aggacgcaga gtccgaaaac aatccagccg
      901 gtgttcatgg tcttggcaag agtcgctgac ccgcgcatgt tactgcggat caaaataatc
      961 gtggcgaaat gttgacttat cctgtgcggt gtctataatg cgcgcttctt caggcggtta
     1021 gctcagttgg ttagagcgcc acgttgacat cgtggaggtc gttggttcga ttccaatacc
     1081 gcctaccaaa ttcctgacgg agtcgttttg atcactttcc gaacttgcac tgcagttcag
     1141 aaactctaat cgagtcggct ttcgttcatc caaaaaaagt gcggctcagc ccgcactttt
     1201 ttttcgtcca gagaatcctg tcatgcccga tatcaaactt cccgatggtt ccatccgttc
     1261 cttcgcgcag ccggtcacca tcgccgaggt ggcagccagc atcggcgccg gtctggcgcg
     1321 tgcggcgctg gccggcaagg tcaatggcaa gctggtcgat acctcgtacc tgattgaaga
     1381 aaatatcgat ctggccatcg tcaccgatcg tgacgccgat ggcctggaac tgatccgcca
     1441 ctcgacggcg cacctcctgg cctacgcggt caaggaattg ttcccggaag cccaggtgac
     1501 catcggcccg gtcgtcgaaa acggcttcta ttacgacttc gcttacaagc gtccgtttac
     1561 gccggaggat ctggttgcca tcgaaaaacg catggccgag ctggccaaga gggatattcc
     1621 ggtctcgcgc gaagtctgga atcgcgacga cgcggtcaat ttcttcctcg gtcagggtga
     1681 aaagtacaag gccgaactga ttgccgcgat tcccgccgat cagcaggttt cgctctaccg
     1741 cgaaggcgat ttcgtcgacc tctgccgcgg tccgcacgtg ccgtcgaccg gcaagctcaa
     1801 ggttttcaag ctgatgaagg tggccggcgc ctactggcgt ggcgatcatc gcaacgaaca
     1861 gctgcagcgc atctacggca ccgcctgggc gaagaaggag gacctcgacg cctacctgca
     1921 catgctggaa gaagcggaga agcgcgacca ccgtcgtctg ggcaagcagt tcgacctgtt
     1981 ccacatgcag gatgaggcgc cgggcctggt tttctggcac cccaagggtt ggtcggtgtg
     2041 gcaggaagtc gagggataca tgcgcaaggt ctaccgcgac aacggctacc aggaagtccg
     2101 ctgcccgcag attctcgatc gctcgctgtg ggagaagtcg gggcactggg atcactacaa
     2161 ggacaacatg ttcacgacct cgtcggaaaa ccgcgattac gcggtcaagc cgatgaactg
     2221 tccgggccat atccaggtgt tcaattccga cctgcgttcc tatcgcgaac tgccgctgcg
     2281 ttacggcgaa ttcggctcct gccaccgcaa cgagccgacc ggcgcgctgc acggcctgat
     2341 gcgcgtgcgc ggcttcgttc aggacgacgg acacatcttc tgtaccgaag accagatcga
     2401 gtccgaagtg acggcgttca acgcgctggt caagaaggtt tatgccgatt tcggcttcaa
     2461 cgacgttgcc gtcaagctgg cgctgcgtcc ggaagggcgg gtcggttccg acgaagtctg
     2521 ggacaaggcc gaggatgcgc tacgtcaggg cttgcgtgct tccggtctgg agtggaccga
     2581 attgccgggt gagggcgctt tctacgggcc gaagatcgaa ttccacatca aggacgcgat
     2641 cggtcgctcc tggcagtgcg gcaccatgca ggtcgacttc tccatgccgg gcc
//
LOCUS       DAOSDX010000357         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13274_length_2692_cov_3.792947, whole genome shotgun sequence.
ACCESSION   DAOSDX010000357 DAOSDX010000000
VERSION     DAOSDX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13274_length_2692_cov_3.792947"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="PLN02_08510"
     CDS             <1..1318
                     /locus_tag="PLN02_08510"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOI52404.1"
                     /translation="ANQAWFEYMPADVSFDDSVAGFATIRLYRDFRFGTLAHLLMTDE
                     RLYRSDHLIPEAAPNPATGSATGSIGSRYMVPETVLYDQVEVAKIAAGTGLGDPLALV
                     SMLGATQRDWWKTTLGNASTTWKLWGSEVMLLKLGLDGTRAIAALIALQAVPTVGAAT
                     AFGVIMPDIVANGVNSAYIPAADKAALAPYFQKFLLNADMWDGYDAERRDLMNFIANN
                     NIANVVALTGDIHAFYAGEVRDNYRAVGGGNPVMNEIVVAGVSSDSFFSYLKGAVAAL
                     SSDLATLVYYPVNDLPVPGVGNISFDVNLLDYTLGRPAPTLADLQQQVRHPVRSALAQ
                     KGVPEAQLDATTDAVLSGLAADPAFNTSLLGLAQTLAGLDGNPWLKHIDSDAQGYAVV
                     TLTPDELVCRLRKMHRLIGSDAPGSAIVATETTARIAAGTKTVTIS"
     gene            1530..1757
                     /locus_tag="PLN02_08515"
     CDS             1530..1757
                     /locus_tag="PLN02_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52405.1"
                     /translation="MSWEIDADVWKLVGVKIKSRWGKINQARLEIIDSKRELLVGQVQ
                     ELYAVSAEEAENQVQSFEKHTKELRPKTAEA"
     gene            complement(1803..>2692)
                     /locus_tag="PLN02_08520"
     CDS             complement(1803..>2692)
                     /locus_tag="PLN02_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52406.1"
                     /translation="AKGLQLTVHNAAMPLGLMGDRTRLQQALLNYLSNAVKFTERGTI
                     TLRSFVLEETRDDVLLRFEVSDTGIGIETAALERLFSAFVQVDSTTTRKYGGTGLGLA
                     ITRKLAELMGGAAGASSVPGQGSTFWFTARLRKGQDQHATAGTPLTQAEAVLGRKYVG
                     TRVLLAEDEPINREIALAMLEDVGLVVDTAADGVEAVQLAGDNDYALMLMDMQMPNMD
                     GLAATRRIRQLASHAHTPILAMTANAFAEDRSRCFDAGMDDFIAKPVRPEQLYLILLN
                     WLDAARSSEDRRSAEKKPT"
BASE COUNT          493 a          959 c          810 g          430 t
ORIGIN      
        1 cgccaaccag gcctggttcg aatacatgcc ggccgacgtc agcttcgacg actcggtcgc
       61 cggctttgcg acgatccgcc tctaccgcga cttccgcttc ggcaccctgg cccatctgct
      121 gatgaccgac gaacgcctct accgcagcga ccacctgatc ccggaagcgg cgcccaaccc
      181 ggccaccggc agcgcgacgg gttcgatcgg cagccgctac atggtccccg aaacggtcct
      241 ctacgatcag gtcgaagtgg ccaagatcgc tgccggcact ggcctgggcg acccgctggc
      301 gctggtttcg atgctcggcg cgacacagcg cgactggtgg aagacgacgc tgggcaacgc
      361 cagcaccacc tggaaactgt ggggctcgga agtcatgctg ctcaagctcg gcctcgacgg
      421 cacgcgcgcc atcgccgcgc tgatcgccct gcaggcggtg ccgacggtag gcgccgccac
      481 cgccttcggc gtgatcatgc cggacatcgt cgccaacggc gtcaattcgg cctacatccc
      541 ggccgccgac aaggccgcgc tggcccccta cttccagaaa ttcctcctca acgccgacat
      601 gtgggacggc tacgacgccg aacggcgcga cctgatgaac ttcatcgcta acaacaacat
      661 cgccaacgtc gtcgccctga ccggcgacat ccacgccttc tacgccggcg aagtgcgcga
      721 caactaccgc gccgtcggcg gcggcaaccc ggtgatgaac gagatcgtcg tcgccggcgt
      781 ctccagcgac tccttcttct cctacctcaa gggtgccgtc gccgcgctct ccagcgacct
      841 cgccacgctg gtctattacc cggtcaacga cctgccggta cccggtgtcg gcaacatcag
      901 cttcgacgtc aacctgctcg actacaccct cggtcgcccg gcgccgacgc tagccgacct
      961 gcagcagcag gtgcgccacc cggtgcgcag cgcgctggcg caaaagggcg taccggaagc
     1021 ccagctcgac gcgacgaccg acgccgtgct cagcggactg gccgccgacc cggccttcaa
     1081 caccagcctg ctcggccttg cccagaccct cgccgggctc gacggcaacc cgtggctcaa
     1141 gcacatcgac agcgacgccc agggctacgc cgtggtcacg ctgaccccgg acgaactcgt
     1201 ctgccgcctg cgcaagatgc accgcctgat cggtagcgac gcccccggca gcgccatcgt
     1261 cgccacggaa accacggccc gcatcgccgc cgggacgaaa accgtgacca tcagctgagc
     1321 ctccggccgg attattttcg ccagcaacgc aaatgagctc agacggccca cccgctttcg
     1381 gttagtctga agcggtagcc gggctgcaat agacgccccg gctaccgctc ctgtccggcc
     1441 ttgacccttt ccttacgagg ctggagcgca acgctggcgg aagcgggcgc caggcaatct
     1501 cgccaggcgc cacctacaag gagctgcaaa tgagctggga aattgatgcg gatgtctgga
     1561 agctggtcgg ggtcaaaatc aagtcgcgct ggggcaagat caaccaggcc cgcctggaga
     1621 tcatcgacag caagcgcgaa ttgctggtcg gccaggtcca ggagctctac gcagtcagcg
     1681 ccgaggaagc cgaaaaccag gtgcagagtt tcgaaaagca caccaaggaa ctgcgcccca
     1741 agacagccga ggcctgaacg ccagacctcc gggcatcgca cggaccgcaa aagccaaccg
     1801 gatcaggttg gctttttttc tgccgatcgt cgatcctcgg aactccgggc ggcatccagc
     1861 cagttgagca ggatcaggta gagctgctcc ggccgcaccg gtttggcgat gaagtcatcc
     1921 atgccggcat cgaaacaacg ggagcggtcc tcggcaaagg cgttggcggt catggccagg
     1981 atcggcgtat gcgcgtgcga cgccagttgc cggatccgcc gcgtcgctgc caacccgtcc
     2041 atgttcggca tctgcatgtc catcagcatc agcgcgtaat cgttgtcgcc cgccagttgc
     2101 accgcctcca cgccgtccgc cgcggtatcg acgaccagac cgacgtcttc gagcatggcg
     2161 agagcgattt cccggttgat cggctcgtct tccgccagca agacgcgggt accgacgtac
     2221 ttgcggccga ggacggcttc cgcctgtgtc aagggcgttc ccgccgtcgc atgctgatcc
     2281 tgccccttgc gcagccgcgc cgtgaaccag aaggtgctgc cctgccccgg cacgctgctg
     2341 gcccccgccg caccgcccat cagttcggcc agttttctgg tgatcgccag ccccagcccg
     2401 gtgccgccat atttccgggt ggtcgtgctg tcgacctgaa cgaaggccga gaacaggcgt
     2461 tcgagcgcag cggtttcgat accgatcccg gtgtcgctga cttcgaaacg cagcagcaca
     2521 tcgtcgcgcg tctcttcgag cacgaaggaa cgcagggtga tcgtgccgcg ctcggtgaac
     2581 ttgacggcat tcgacaggta attgagcagg gcttgctgca gacgtgtccg gtcgcccatc
     2641 aagccaagcg gcatggcggc gttgtggacc gtcagctgca gccccttggc ac
//
LOCUS       DAOSDX010000358         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13319_length_2685_cov_3.034601, whole genome shotgun sequence.
ACCESSION   DAOSDX010000358 DAOSDX010000000
VERSION     DAOSDX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13319_length_2685_cov_3.034601"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLN02_08525"
     CDS             complement(<1..336)
                     /locus_tag="PLN02_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOI52407.1"
                     /translation="MLAPIEHRIALELNVRPAQVKAAVQLLDEGATVPFIARYRKEVT
                     GELDDTQLRTLEERLGYLRELEDRRGAVLASIEEQGKLTPELRGEIENAETKQRLEDL
                     YLPYKPKRRT"
     gene            456..893
                     /gene="fliW"
                     /locus_tag="PLN02_08530"
     CDS             456..893
                     /gene="fliW"
                     /locus_tag="PLN02_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOI52408.1"
                     /translation="MQVNTYLFGTVDVAEDKVISFPNGLIGFEDKKQFMLAHEEGKGE
                     PQSFTLQSVDDAALAFQIIDPTALGFNYELELTDEENALLQSPAADDVAVMITLYKQE
                     EGGGVAPSLRAPLIINLKARVGLQKLLAQMTTNVTLSNLVNKV"
     gene            complement(934..1686)
                     /locus_tag="PLN02_08535"
     CDS             complement(934..1686)
                     /locus_tag="PLN02_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOI52409.1"
                     /translation="MGLFDWLRRRPAAIPDDLWQRTLASQPFLAALDADEARRLRALA
                     ERFLADKEFTAAGGLELSDEICVAIAAQGCLPVLELGLGAYGDWVGIVVYADEFVVPR
                     EVADDDGVVHRYADVLSGEAWEGGPLLVSWRDVQMAGGDYNVVIHEFAHKLDMLNGEV
                     DGIPALHSGITAERWEGVLLPAYDDFCARVDGGEETIIDPYAGEAPGEFFAVLSECFF
                     GIPDVVAAEYPALYELLRDYFRQDPLARLQKK"
     gene            complement(1694..2581)
                     /locus_tag="PLN02_08540"
     CDS             complement(1694..2581)
                     /locus_tag="PLN02_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI52410.1"
                     /translation="MPKFLTNPYLLLTLTALFWSGNMVLGRGIRADVPPLALAFWRWA
                     IAFLLVLPLALPHFKAQWPLLKKGWRPVLLLGLLGVGGYNTFAYLALQTTTATNAVLL
                     NSFIPVATIAISWAFLGKRLTPLAASGVIVSLLGALAIVARGELGVLLHLNLNIGDLW
                     MLGAVAVWAIYTVGLAWRPAGVHPMLALAAFTLVGLAALFPAYLWEMAQGRTINVHAG
                     SLLSLAYVGIFPSFVGYIFYNRGVAEVGANKASLFIHLMPVFGTLLSVVFLGEIPHPY
                     HYAGIALIFAGIWLTMKKG"
BASE COUNT          501 a          797 c          956 g          431 t
ORIGIN      
        1 ggtgcggcgc tttggcttgt agggcaggta gaggtcttcg aggcgctgct tggtttcggc
       61 gttttcgatc tcgccgcgca gttccggcgt cagcttgccc tgttcctcga tgctggccag
      121 caccgcgccg cggcggtctt cgagttcgcg caggtaaccg aggcgctctt ccagcgtgcg
      181 cagttgcgtg tcgtcgagtt cgccggtgac ttccttgcgg tagcgggcga tgaaaggcac
      241 ggtggcgcct tcgtcgagga gctggacggc ggccttgacc tgggccggac ggacgttgag
      301 ttcgagggcg atgcggtgtt cgatgggagc gagcatgggg gcggcgactg ctgaaggggg
      361 cggcaatgat gccgtaatct gaaggtgaat tacaactctc tgcggagtgg tgtaggatgc
      421 caattgatac atcccaactc aagaggagag agcacatgca agtgaatacc tatctgtttg
      481 gtaccgtcga tgtggcagaa gacaaggtca tcagctttcc gaacggcctg atcggtttcg
      541 aagacaagaa gcagttcatg ctcgctcacg aagaaggcaa gggcgagccg cagagcttca
      601 cgctgcagtc ggtggatgac gcggcgctgg ctttccagat catcgatccg accgcgctcg
      661 gcttcaacta cgagctggaa ctcaccgacg aggaaaacgc gctgctgcag tcgccggccg
      721 ccgacgacgt ggcggtgatg atcaccctct acaagcagga agaaggcggc ggcgtcgccc
      781 ccagcctgcg cgcgccgctg atcatcaacc tcaaggcccg cgtcggcctg cagaagttgt
      841 tggcgcagat gaccaccaat gtcacgctgt ccaacctggt gaacaaggtc tgattcctcg
      901 acacccacgc caagccggcc tgcgcgccgg cttttatttt ttctgcaggc gggctaacgg
      961 gtcctggcgg aagtaatcgc gtagtagttc gtaaagcgcc gggtattcgg cggcgacgac
     1021 atcgggaatg ccgaagaagc attcactgag gacggcgaag aattctccgg gtgcctcgcc
     1081 ggcatagggg tcgatgatcg tctcctcgcc gccgtcgacg cgggcgcaga aatcgtcgta
     1141 agcgggaagg agaacgcctt cccagcgttc ggcggtgatt ccggaatgca gcgccgggat
     1201 gccgtcgact tcgccgttga gcatgtcgag cttgtgcgcg aactcgtgga tgacgacgtt
     1261 gtagtcgccg ccggccatct gcacgtcgcg ccaggaaacg agcagcgggc cgccttccca
     1321 ggcctcgccg gagagcacgt cggcgtaacg gtgcaccacg ccgtcgtcgt cggcgacctc
     1381 acgcgggacg acgaactcgt cggcatagac gacgatgccg acccagtcgc cgtaggcgcc
     1441 gaggccgagt tcgagcaccg gcaggcaacc ctgggcggcg atggcgacgc agatttcgtc
     1501 gctcagttcg agcccgccgg cggcggtgaa ttccttgtcg gccaggaagc gttcggccag
     1561 cgcccgcagg cggcgcgcct catcggcgtc gagcgcggcg aggaagggct ggctggccag
     1621 cgtccgttgc cagaggtcgt cggggattgc cgccggacgg cggcgcagcc agtcgaacag
     1681 gcccatccgc cgctcagcct ttcttcatcg tcagccagat gccggcgaag atcagcgcga
     1741 tcccggcata gtggtagggg tgcgggattt cgcccagaaa gacgaccgag agcagggtgc
     1801 cgaacaccgg catcaggtgg atgaacaggc tggccttgtt ggcgccgact tcggcaacgc
     1861 cgcggttgta gaagatgtag ccgacgaagc tcgggaaaat gccgacgtag gcgagcgaga
     1921 gcagcgagcc ggcatgcacg ttgatcgtcc gcccttgcgc catctcccac aggtaggcgg
     1981 gaaacagggc ggcgaggccg accagcgtga acgcggcgag cgccagcatc gggtgcacgc
     2041 cggccgggcg ccaggccagg ccgacggtgt agatggccca gacggcgacg gcaccgagca
     2101 tccagaggtc gccgatgttg aggttgagat ggagcagcac gcccagttcg ccgcgggcga
     2161 cgatggccag cgcgccgagc agcgaaacga tgacgccgct agccgccagc ggcgtcagcc
     2221 gcttgccgag gaaggcccag gaaatggcaa tggtggcgac cggaatgaag gagttgagca
     2281 gcaccgcgtt ggtcgccgtc gtcgtctgca gcgccagata ggcgaaggtg ttgtagccgc
     2341 cgacgccgag caggccgaga agcagcaccg gccgccagcc ttttttcagc agcggccact
     2401 gcgccttgaa atgcggcaac gccagcggca ggacgagcag gaaggcgatc gcccagcgcc
     2461 agaaggcgag cgccagcggc ggcacgtcgg cgcggatgcc gcggccgagc accatgttgc
     2521 ccgaccagaa gagcgcggtc agggtcagca gcaggtaagg gttggtcagg aatttcggca
     2581 tggtcggcgg gatgcggcac cgtcgaccac gggttcgacg gtggggtgga tcaggggtag
     2641 agcccccgtt cggcgctggc ttcgaggacc cggtggcagg cgatg
//
LOCUS       DAOSDX010000359         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13335_length_2683_cov_4.245434, whole genome shotgun sequence.
ACCESSION   DAOSDX010000359 DAOSDX010000000
VERSION     DAOSDX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13335_length_2683_cov_4.245434"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLN02_08545"
     CDS             <1..693
                     /locus_tag="PLN02_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52411.1"
                     /translation="ASVGNDYGPELAPLAQELDLVLARNARIVDRARGHAADLSHALK
                     KPLSLLAADGELQRNALLRQQVGAMTQLIDRHLARAGSGAGSVRQVAVGDCIDSLIGL
                     MQRLHRERGLSWQLDVNRDLHWRGEQTDFEEMLGNLLDNAGKWARSRVSVRARRQHDE
                     VVVEIGDDGPGLSAEQIRETGVRGRRFDEDVEGSGLGLAITGDIAETYAGELSLGRSP
                     LGGLLACLRLPG"
     gene            779..>2683
                     /locus_tag="PLN02_08550"
     CDS             779..>2683
                     /locus_tag="PLN02_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOI52412.1"
                     /translation="MPQAAIPASPISRLWQRLDFCGPYAGIAMLFLVGLPLLTLSRAA
                     LMLWQSERVAATGIWSTMLLQGVRVDVIQLSLLALPLLLLAPLLAGKRLWPAWRRLNV
                     GWMTLMIAALALLEIASPAFINEYDSRPNRLFVEYLKYPNEVVAMLWSGFRLHLVGGL
                     LVTGLLGWSAWRIMRAWQRREAPWSGWKLWLSWPLLVLICALAIRSSLQHRPANPATF
                     ALTADPMVNTLILNSAWSVTHAIYGMQHEANSSEIYGKMESADMLAEFRHARTSLGNP
                     HPLVGDPEIPTLVDQQATVRRAKPLNLVIILEESLGATFVQSLGGVPVTPNLEKLKEQ
                     GWWFEQLYATGTRSIRGIEAVTTGFLPTPAQAVVKLSLAQRNFATLASILGRHGYESE
                     FIYGGEAHFDNMRGFFTGNGFTHITEQKDFKNPKFVAGWGVSDEDLFDKTHERLNAKH
                     AEGQPSFTLVFTSTNHSPFDFPDGRIELYEQPRATDNNAVKYTDHALGEFIAKAKASP
                     YWQDTLFVVVADHDIRVRGDSMVPVERFHIPGLILGADLKPKRVKTVASQIDLAPTLL
                     SLMGVDTKHPMPGRDLTREADDLPGRAMMQYEQNYGWMEGNQVVVLRPEKTPAFAHYD
                     RQTKRLSAAEA"
BASE COUNT          471 a          973 c          818 g          421 t
ORIGIN      
        1 gccagcgtcg gcaacgacta cggcccggaa ctggcgccgc tggcccagga actcgacctg
       61 gtgctggccc gcaacgcgcg catcgtcgac cgcgcccgcg gccacgccgc cgacctcagc
      121 cacgcgctga aaaagccgct gtcgctgctc gccgccgatg gcgaactgca gcgcaatgcc
      181 ctgttgcgcc agcaggtcgg cgccatgacc cagttgatcg accgccacct ggcccgcgcc
      241 ggcagcggtg ccggcagcgt ccgccaggtg gcggtcggcg actgcatcga cagcctgatc
      301 ggcctgatgc agcgcctgca ccgcgaacgt ggcctgagct ggcagctcga cgtcaaccgc
      361 gacctgcact ggcggggcga acagaccgat ttcgaggaaa tgctcggcaa cctgctcgac
      421 aacgccggca aatgggcaag atcgcgggtc agcgtgcgcg cccgccggca gcacgacgaa
      481 gtcgtcgtcg aaatcggcga cgacggcccc ggcctgtcgg cggaacagat ccgcgaaacc
      541 ggcgtacgcg gccgacgctt cgacgaagac gtcgaaggca gcggcctcgg cctggcgatc
      601 accggcgaca tcgccgaaac ctacgcaggc gagctgagcc tcggccgttc accgctcggc
      661 ggcctgctcg cctgcctgcg actaccgggc tgaggcacaa tagcgctccc ggacgaactt
      721 aatggccgtt taatcgatct gatccagact tttccggccc gcttccaacg agatttccat
      781 gcctcaagcc gcaattcccg cctcccctat ctcccgtctc tggcaacgcc tcgatttctg
      841 cggcccctac gccggcatcg ccatgctctt cctggtcggc ctgccgctgc tgacgctgtc
      901 gcgcgcggcg ctgatgctgt ggcagtcgga gcgggtcgcc gccaccggca tctggagcac
      961 gatgctgctg cagggcgtgc gtgtcgacgt gatccagctt tccctgctcg ccctgcccct
     1021 gctgctgctc gccccgctgc tcgccggcaa gcgcctgtgg ccggcctggc gcaggctgaa
     1081 cgtcggctgg atgacgctga tgatcgccgc cctggcgctg ctcgagatcg cctcgccggc
     1141 cttcatcaac gaatacgaca gccggccgaa ccggctcttc gtcgaatacc tgaaataccc
     1201 caacgaagtc gtcgccatgc tgtggagcgg cttccgcctg cacctggtcg gcggtctgct
     1261 ggtcaccggc ctgctcggct ggtcggcgtg gcggatcatg cgtgcctggc aacggcgcga
     1321 ggcgccgtgg tcgggctgga aactgtggct gagctggccg ctgctggtgc tgatctgcgc
     1381 cctggccatc cgttccagcc tgcagcaccg gccggccaac ccggccacct tcgcgctcac
     1441 cgccgacccg atggtcaata cgttgatcct gaattccgcc tggtcggtga cgcacgcgat
     1501 ctacggcatg cagcacgagg ccaattcgag cgagatctac ggcaagatgg aaagcgccga
     1561 catgctcgcc gagttccgcc acgcccgcac cagcctcggc aacccgcatc cgctggtcgg
     1621 cgacccggaa attccgaccc tggtcgacca gcaggcgacg gtacgccgtg ccaagccgct
     1681 gaacctggtg atcatcctcg aggaaagcct gggcgcgacc ttcgttcagt cgctcggcgg
     1741 cgtcccggtg acgcccaacc tggaaaagct gaaggaacaa ggctggtggt tcgagcagct
     1801 ctacgccacc ggcacccgct cgatccgcgg catcgaggcg gtgaccaccg gcttcctgcc
     1861 gacgccggcg caagccgtcg tcaagctgtc gctggcccag cgcaattttg cgacgctggc
     1921 ttccatcctc ggccgccacg gttacgaatc ggagttcatc tacggcggcg aagcgcattt
     1981 cgacaacatg cgcggcttct tcaccggcaa cggtttcact cacatcaccg aacagaagga
     2041 cttcaagaac ccgaagttcg tcgccggctg gggcgtttcc gacgaagacc tgttcgacaa
     2101 gacgcacgag cgcctgaacg ccaagcacgc cgagggccag cccagcttca cgctggtgtt
     2161 cacctcgacc aaccattcgc ccttcgactt ccccgacggc cgtatcgagc tttacgagca
     2221 acccagggcg accgacaaca acgccgtcaa atacaccgac catgccctcg gcgagttcat
     2281 cgccaaggcc aaggccagtc cctactggca ggacaccctg ttcgtcgtcg tcgccgacca
     2341 cgacatccgc gtgcgtggcg acagcatggt accggtcgag cgcttccaca tccccggcct
     2401 gatcctcggc gccgacctca aaccgaagcg ggtgaagacc gtcgccagcc agatcgacct
     2461 ggcgccgacg ctgctctcgc tgatgggcgt cgacaccaaa cacccgatgc cgggtcgcga
     2521 cctgacgcgc gaagccgacg acctgcctgg ccgggcgatg atgcagtacg agcagaatta
     2581 cggctggatg gaaggcaacc aggtcgtcgt cctgcgcccg gaaaaaaccc cggccttcgc
     2641 ccactatgac cgccagacaa agcgcctgag cgccgccgag gcg
//
LOCUS       DAOSDX010000360         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13342_length_2682_cov_3.763228, whole genome shotgun sequence.
ACCESSION   DAOSDX010000360 DAOSDX010000000
VERSION     DAOSDX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13342_length_2682_cov_3.763228"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..498
                     /locus_tag="PLN02_08555"
     CDS             82..498
                     /locus_tag="PLN02_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOI52413.1"
                     /translation="MSFGSFNEGGNAPMADINTTPLVDVMLVLLVIFIITAPLFHQAI
                     PIDLPRVSSTKLDDKPEVIQLAINADGIAYWNGEAVHRNALDARFAEASARQPELHLR
                     ADREARYEAVADVMAAAQRSGIVKIAFVTQPKGEAK"
     gene            540..2615
                     /locus_tag="PLN02_08560"
     CDS             540..2615
                     /locus_tag="PLN02_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI52414.1"
                     /translation="MKSRQRLLAVAVATAFVQPASAADDGDRVLSPVKAEAAREVEVQ
                     SRTELGRLTEYTPLAGSVVEREELETVRFVDSFHELLPRVPGVSMSRNMRFTDGGKNY
                     TENRIDGMRARNTGTYGFVDQVNSGDIERIEFIRGPGSVLSGSNAIGGTINVITRDPP
                     AKREFQVSGEAFEDGGYRTGLSGGDRLSDSLGYFFNINQLDHDGWRDHTDQKKTSLST
                     KWVWRPDSSSKLALRYEYIDDDYQSAGSLDEAQFRANWRQAEPDSYLRTAVKYSTPSL
                     HYRKMFGDFGELNLYGQQRHTDQTARSSGFSGGGATLGDTESYETNFQLIYKHNFSLA
                     KTSVTGGFDYLDTASDSRKFKDLSPNSFHFSRGALTGYSKSYEKNKSPFLQLEFSPLD
                     PLRLTLGVRRDDISYRIDDKINDNKDGSASYQKTVRKFGAVYEISPRALIWANVAEGF
                     MGPGVSTLLGSGTPTPATPAAAWASRYIPANMDLKPEESMTHEIGVRGSFDSGLKYDT
                     GYYETDFENLIVSQACIQNVDYCRTRNVNAAKASAHGFETALSYDINKYLEVGLSHTY
                     ARYRYGDYKTASADYTGTRRYYTPRNHYNLRVVVKPAAGWRVELEGDRIDSYYTNQAN
                     TDRYSRPDLYNLRASYSAKSWSAWLHVFNLMDSKYAERVGSTDAGVRNSFDAGYTPRL
                     IRVGLSYQF"
BASE COUNT          547 a          931 c          778 g          426 t
ORIGIN      
        1 acggcttcgc ccacgacctg cacgcctacc tgaccaccgg cacccgcatc acgctggcgc
       61 gggcggcctg aggagcgcgc gatgtccttc ggttccttca acgagggcgg caacgccccg
      121 atggccgaca tcaacaccac cccgctggtc gacgtcatgc tcgtcctgct ggtcatcttc
      181 atcatcaccg cgccgctctt ccaccaggcg attccgattg acctgccgcg cgtcagctcg
      241 accaaactcg acgacaagcc cgaggtcatc cagctggcga tcaacgccga cggcatcgcc
      301 tactggaacg gcgaggcggt gcaccgcaac gcgctcgacg cccgcttcgc cgaagccagc
      361 gcccgccagc ccgaactgca cctgcgcgcc gaccgcgaag cgcgctacga agccgtcgcc
      421 gacgtgatgg ccgcggcgca gcgcagcggc atcgtcaaga tcgccttcgt cacgcagccc
      481 aagggcgagg cgaagtgaat cgctcattca ttccataaga aaacaccagg gggtagttca
      541 tgaaatccag gcaacgcctg cttgccgtcg ccgtggcgac cgcgttcgtc caaccagcgt
      601 ccgccgccga cgacggcgac cgcgtcctgt caccggtcaa ggccgaagcg gcgcgtgaag
      661 tcgaagtcca gtcgcgcacc gaactcggcc gcctgactga atacacgccg ctcgccggca
      721 gcgtcgtcga gcgcgaggag ctggagacgg tgcgcttcgt ggactccttc cacgaactgc
      781 tgccgcgcgt gcccggcgtc agcatgagcc gcaacatgcg cttcaccgac ggcggcaaga
      841 actacaccga aaaccgcatc gacggcatgc gtgcccgcaa caccggcacc tacggtttcg
      901 tcgaccaggt gaactcgggc gatatcgagc gcatcgagtt catccgcggg ccgggctcgg
      961 tattgagcgg cagcaacgcg atcggcggca cgatcaacgt gatcacccgc gatcccccgg
     1021 ccaaacggga attccaggtc agcggcgaag ccttcgagga cggcggctac cgtaccggcc
     1081 tcagcggcgg cgaccggctc agcgacagcc tcggctattt cttcaacatc aaccagctgg
     1141 accatgacgg ttggcgtgac cacaccgatc agaagaagac ctcgctcagc accaagtggg
     1201 tctggcgtcc ggacagcagc tccaagctgg cgctgcgcta cgaatacatc gacgacgact
     1261 accagagcgc cggttccctt gatgaagcgc agttccgtgc caactggcgg caggccgagc
     1321 cggatagcta cctgcgcacg gcggtcaagt attcgacgcc gtcgctgcac taccgcaaga
     1381 tgttcggcga cttcggcgag ctgaacctct acggccagca gcgccacacc gatcagacgg
     1441 cgcgctcgtc gggattctcc ggcggcgggg cgacgctcgg cgacaccgaa tcgtacgaga
     1501 ccaatttcca gctgatctac aagcacaatt tcagcctggc caagacctcg gtcaccggcg
     1561 gtttcgatta tctggacacg gccagcgaca gccggaaatt caaggatctc agcccgaact
     1621 cctttcattt ctcgcgcggc gcgctgaccg gctattcgaa atcctatgaa aagaacaagt
     1681 cgcccttcct gcagctcgag ttctcgccgc tcgatccgct gcgcctgaca ctgggcgtgc
     1741 ggcgcgacga catcagttac cgcatcgatg acaagatcaa cgacaacaag gacggcagcg
     1801 ccagctacca gaagacggtg cgcaagttcg gcgccgtcta tgaaatctcg ccgcgcgccc
     1861 tgatctgggc gaacgtcgcc gagggcttca tgggtccggg cgtcagcacc ctgctcggca
     1921 gcggcacacc gaccccggcg acgcctgcgg cggcctgggc gtcccgctac ataccggcca
     1981 acatggacct gaagccggaa gagtcgatga cccacgaaat cggtgtgcgt ggcagcttcg
     2041 attccggcct caagtacgac accggctatt acgagaccga cttcgagaac ctgatcgttt
     2101 cccaggcctg catccagaac gtcgattatt gccgtacgcg taacgtcaat gccgccaagg
     2161 ccagcgccca cggcttcgag acggcgcttt cctacgatat caacaagtat ttggaagtcg
     2221 gcctgtcgca cacctacgcc cgttatcgct atggcgacta caagacggcg agcgccgact
     2281 acaccggcac ccggcgctac tacacgccgc gcaaccacta caacctgcgc gttgtggtca
     2341 agccggctgc cggctggcgg gtcgagctgg aaggcgatcg catcgacagc tattacacca
     2401 accaggccaa taccgaccgc tacagccgtc ccgacctcta caacctgcgc gccagctaca
     2461 gcgccaagtc gtggagcgcc tggctgcatg tcttcaacct gatggacagc aagtacgcgg
     2521 aacgcgtcgg ctccaccgac gccggcgtgc gcaactcctt cgacgccggc tacacgccgc
     2581 gtctgatccg ggtcgggctg tcctaccagt tctgatgcaa aaccgtattt ctctaggtga
     2641 attcatgtcg gaaaagaacg ttcgggatgg cgcagcgaat gc
//
LOCUS       DAOSDX010000361         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13367_length_2679_cov_2.348704, whole genome shotgun sequence.
ACCESSION   DAOSDX010000361 DAOSDX010000000
VERSION     DAOSDX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13367_length_2679_cov_2.348704"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PLN02_08565"
     CDS             complement(<1..857)
                     /locus_tag="PLN02_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOI52415.1"
                     /translation="MSEFHQGEQDPDRFRRRILFAGAFVLTCFALLFGRFFYLQILQH
                     EYYRTRAESNRISLVPVMPNRGNIVDRNGVILARNYSAFTLEITPSKTLGLEETIDRL
                     GEIVEIQLKDRRRFKRLLEESKNFESIPIRTRLTDEEVARFAAQRYRFPGVEVKARLF
                     RQYPENTLAAHAIGYISRINTKDKEMIEANGHDANYKGTEHIGKVGLEANYEFELHGT
                     AGYEQVEVDAGGRAVRVLSQTAPIPGNNLTLTLDARLQSVAEQAFGGRRGALTAIDPS
                     NGAVLALVSS"
     gene            complement(867..1388)
                     /gene="mreD"
                     /locus_tag="PLN02_08570"
     CDS             complement(867..1388)
                     /gene="mreD"
                     /locus_tag="PLN02_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOI52416.1"
                     /translation="MQPTFSSSRILLPVRPWFIVASLFAALLANFLPTAHWPGIPDWV
                     ALVLCFWCIRETRRVGMGWAFFLGLAMDVADGAVLGQHCFAYVLLAYFAAGQSRRILW
                     FPLAQQALQIFPLLLACQVIQLLMRLPTGADFPGWGYLIGPLSATLLWIPATFLLLLP
                     QYQPVERDANRPL"
     gene            complement(1388..2302)
                     /gene="mreC"
                     /locus_tag="PLN02_08575"
     CDS             complement(1388..2302)
                     /gene="mreC"
                     /locus_tag="PLN02_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOI52417.1"
                     /translation="MAGSDHAPPPFFKRGPAPLALLTFYIALSLALFFVDLRLRSLDL
                     LRQTVALITDPIQRIAQMPGGLVDNAGDYLRGLDKLLEENRSLKQAQLQTAPNLLRLE
                     QLESENDRLRKLLAVKEREKARGEVARIMYTARDPFTRRIVIDKGQQSGIVAGQPAID
                     EAGVVGQVTRVFPFSAEITLITDKDQTVPVQVVRSGQRSVVFGLGSGELELRYIPANA
                     DIREGDLLVTSGLDGVYLAGFPVAKVVSIERDNSYAFARIRCAPVAAVENFGEVLVLA
                     PRASQPPPPPDSPGRAGNSGTPGQEEKK"
     gene            complement(2334..>2679)
                     /locus_tag="PLN02_08580"
     CDS             complement(2334..>2679)
                     /locus_tag="PLN02_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOI52418.1"
                     /translation="EMEVSGINKAEGIPRKFTISSNEILEALTDPLNQIISAVKSALE
                     KTPPELGADIAEKGMVLTGGGALLRDLDRLLMEETGLPVIVADEPLTCVARGCGMALE
                     KMDKLGSIFASD"
BASE COUNT          454 a          768 c          983 g          474 t
ORIGIN      
        1 gaggagacga gggcgagtac cgcgccgttg ctcgggtcga tcgccgtcag cgcgccacgg
       61 cggccgccga aagcctgttc ggccaccgac tgcagacggg cgtcgagggt cagggtcagg
      121 ttgttgccgg ggatcggcgc agtttgcgaa aggacgcgca cggcccggcc gccggcatcg
      181 acctcgacct gctcgtagcc ggcggtgcca tgtagttcga actcgtaatt ggcttccagc
      241 cctaccttgc cgatgtgctc ggtgcccttg tagttggcat cgtgcccgtt ggcctcgatc
      301 atctccttgt ccttggtatt gatccggctg atgtaaccga tggcgtgggc ggccagggtg
      361 ttttcgggat actggcggaa cagccgggcc ttcacctcga cgccggggaa acgataacgc
      421 tgcgcggcaa agcgggcgac ttcctcgtcg gtgaggcggg tgcggatcgg aatcgactcg
      481 aaattcttcg attcctcgag caggcgtttg aaacggcggc ggtccttgag ctggatttcg
      541 acgatttctc ccaggcggtc gatggtttct tcgagtccca gggttttcga cggcgtgatt
      601 tccagcgtga aggcggaata gttgcgggcc aggatgacgc cgttgcggtc aacgatgttg
      661 ccccggttgg gcatcaccgg aaccagcgag atgcggttgc tctcggcgcg ggtgcggtaa
      721 tactcgtgct gcaggatctg cagatagaag aagcggccga agagcagggc gaaacaggtc
      781 aggacgaagg cgccggcgaa cagaatccgg cggcggaaac ggtcgggatc ctgttctccc
      841 tggtggaatt cgctcatggt caggggtcag agcgggcggt tggcgtccct ttccaccggc
      901 tggtactggg gcagcagcaa caggaaggtg gcgggaatcc agagcagggt ggcgctcagc
      961 gggccgatca ggtaacccca gccggggaag tcggcgccgg tcggcaggcg catcagcaac
     1021 tggatgacct ggcaggcgag cagcagcgga aagatttgca gcgcctgctg ggccagcggg
     1081 aaccacagga tgcggcgcga ctggccggcg gcgaagtaag cgagcaggac gtaggcgaag
     1141 cagtgttggc cgagcaccgc gccgtcggcg acgtccatcg ccaggccgag gaagaaggcc
     1201 cagcccatgc cgacccgccg ggtctcgcgg atacaccaga aacagaggac cagcgcgacc
     1261 cagtccggga tgccgggcca gtgggcggtc ggcaggaagt tggcgaggag cgcggcgaac
     1321 aggctggcga cgatgaacca ggggcggacc ggcagcagga tgcgcgagga ggagaaggtc
     1381 ggttgcatca ttttttctct tcctggcccg gcgtgccgct gttgccggcg cggcccggcg
     1441 aatccggcgg tggcggcggc tgggaggcgc gcggtgcgag gaccaggact tcgccgaagt
     1501 tctcgactgc ggcgaccggt gcgcaacgga tgcgggcaaa ggcgtaggag ttgtcgcgct
     1561 cgatgctgac caccttggcc accgggaagc cggcaaggta gacgccgtcc aggcccgagg
     1621 tgacgagcag gtcgccttcg cggatgtcgg cgttggcggg aatgtagcgc agttcgagtt
     1681 cgccgctgcc gaggccaaag acgaccgagc gctggccgct gcgcacgacc tgaacgggga
     1741 cggtctggtc cttgtcggtg atcaaggtga tttcggcaga aaaggggaag acgcgggtca
     1801 cctggccgac gacgccggct tcgtcgattg ccggctggcc ggcgacgatg ccagattgct
     1861 ggcctttgtc gatgacgatg cggcgggtga aggggtcgcg cgccgtatac atgatgcgtg
     1921 ccacctcgcc gcgcgctttt tcccgctcct tgacggcgag cagtttgcgc aggcggtcgt
     1981 tctcgctttc cagctgctcc aggcgcagca ggttgggggc ggtctgcaac tgggcttgct
     2041 tcaggctgcg gttttcctcg agcagcttgt ccagtccgcg caggtaatcg ccggcgttgt
     2101 cgaccaggcc gcccggcatc tgggcgatgc gctggatcgg atcggtgatc agggcgacgg
     2161 tctggcgcag cagatcgagg ctgcgcagac ggaggtcgac gaaaaacagc gccaatgaca
     2221 gcgcgatgta gaaagtcaga agggccagcg gtgctggccc tcgcttgaag aacggtggcg
     2281 gtgcgtggtc ggagccggcc atcgcttaat cctgcaggtg ggcggttggg tggtcagtcc
     2341 gaggcaaaga tgctgcccag cttgtccatc ttttccagcg ccatgccgca gccgcgggcg
     2401 acgcaggtca gtggttcgtc ggcgacgatc accggcagac cggtttcttc catcagcagg
     2461 cggtcgaggt cgcgcagcag cgcgccgccg ccggtcagga ccatgccctt ttcggcgatg
     2521 tcggcgccga gttccggcgg cgtcttttcc agcgccgact tgaccgcgga aatgatctgg
     2581 ttgagcggat cggtcagcgc ttcgaggatt tcgttcgacg aaatcgtgaa cttgcgcggg
     2641 atgccttcgg ccttgttgat gccggagact tccatttcc
//
LOCUS       DAOSDX010000362         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13401_length_2673_cov_2.553858, whole genome shotgun sequence.
ACCESSION   DAOSDX010000362 DAOSDX010000000
VERSION     DAOSDX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13401_length_2673_cov_2.553858"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLN02_08585"
     CDS             complement(<1..380)
                     /locus_tag="PLN02_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI52419.1"
                     /translation="MPQFFIKRPVFAWVIALFIVLAGVIAIPQLPIARYPSVAPPSVS
                     LYASYPGATPQTLNDSVVTLVERELSGVKNLLYFESSVDTSGSAQITATFKPGTDPEL
                     AQVDVQNRIKSVEPRLPQAVRQNGL"
     gene            complement(397..1530)
                     /locus_tag="PLN02_08590"
     CDS             complement(397..1530)
                     /locus_tag="PLN02_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI52420.1"
                     /translation="MSSIKGFARILFAGALLLGVAACGPAAENKAPAAPLQVSVMTLE
                     AQALTVSEDLPGRVAALRSAEVRAQVGGIVQHRRFEQGSEVKAGSMLFQINPAPFAAE
                     ADSAAAALQRSEAAHTRAAIQVERMAPLMRAEAVSRQAYDDAVALRDQAAAELAQAKA
                     TLARRRLDLRFASVEAPISGRVDQALVSEGALVAPGDASPMARIQQIDQVYVDVRQSA
                     AALESLRQQAEPTASALAVDILDGEGKPMELSGRILFSGIEVDAGTGDVLLRVLVDNP
                     QRRLLPGMFVRARVRRAHYPAALSVPQQAVQRRGNQASVWVVDMRGLAQQRAIELGEL
                     VGRRYRVVSGLSAGQQVVIEGIERLSPDAPVVTRAWQPAAAGH"
     gene            1670..2410
                     /locus_tag="PLN02_08595"
     CDS             1670..2410
                     /locus_tag="PLN02_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52421.1"
                     /translation="MPESSPTAARPPAAPAQALVLIAEDEPEIAEILSAYLQRSGLRT
                     RHASDGQQALTMHRALKPDLLLLDVQMPQLDGWKVLSEIRTRGDTPVIMLTALDQDID
                     KLMGLRLGADDYVVKPFNPAEVVARVEAVLRRTRAAPTAATPILRAGPFQIDPDSHEA
                     SVYRDGRQQMLDLTLTEFKLLACLMRAPKRVFSRSELLANCLPEGDTQERTVDSHVSK
                     LRKKLEALGIEGVPTSVWGVGYRFGGDA"
     gene            2407..>2673
                     /locus_tag="PLN02_08600"
     CDS             2407..>2673
                     /locus_tag="PLN02_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HOI52422.1"
                     /translation="MTPSHSLRRQIIQSFVVMALGIILISVFGSYAFYALALAYLPGS
                     ISDTWIPSQVETVWIVCTVAAALAMAVFAAIRLSRRILTPLNSVA"
BASE COUNT          444 a          947 c          860 g          422 t
ORIGIN      
        1 aggccgttct gcctgaccgc ctgcggcagg cgcggctcga ccgacttgat gcgattctgc
       61 acgtccacct gggccagctc ggggtcggtg cctggcttga aggtggcggt gatctgcgcg
      121 ctgcccgagg tatcgaccga ggattcgaag tacagcaggt tcttgacccc cgacagttcg
      181 cgttcgacca gggtcaccac cgagtcgttg agcgtctgcg gcgtggcgcc cggataactg
      241 gcgtagagac tgaccgacgg cggcgccacc gagggatagc gggcaatcgg cagttgcgga
      301 atggcgatca cgccggcgag cacgatgaac aatgcgatca cccaggcgaa caccgggcgc
      361 ttgatgaaaa actgcggcat ggcgggcgct cccgactcag tgaccggcgg cagcgggctg
      421 ccaggcacgg gtgacgaccg gcgcatccgg actgaggcgt tcgatccctt cgatcaccac
      481 ctgctgcccg gcgctcagcc cggagactac gcggtagcgg cgcccgacca gttcgcccag
      541 ctcgatcgcc cgctgctgcg caaggccgcg catatcgacc acccagacgc tggcctggtt
      601 tccccggcgc tgcaccgcct gctgcggcac gctcaaggcc gccggatagt gcgcgcgtct
      661 gacgcgggcc cggacaaaca tgccgggcag caagcgacgc tgcgggttgt cgaccaggac
      721 ccgcagcagt acgtcgccgg ttccggcgtc cacctcgatc ccggaaaaca ggatgcgccc
      781 gctcagttcc atgggctttc cttcgccgtc cagaatgtcc acggccagtg cagaagcggt
      841 cggctcggcc tgctggcgca gagattccag cgccgccgcc gactggcgga catcgacata
      901 gacctggtcg atctgctgga ttctcgccat tggcgaagca tcgcccggcg ccaccagcgc
      961 accttcgctg accagcgcct gatcgacccg tccggagatc ggtgcctcga cgctggcaaa
     1021 cctcaggtcc agacgtcggc gcgccagcgt tgccttggcc tgggccagtt cggccgccgc
     1081 ctggtcgcgc agcgctaccg cgtcgtcgta agcctggcgg ctgaccgcct cggcccgcat
     1141 cagcggggcc atccgctcga cctggattgc ggcccgcgta tgcgccgcct cgctccgctg
     1201 caaggcggcg gcggcactgt cggcttcggc cgcgaacggc gccggattga tctggaacaa
     1261 catgctgccg gccttgactt cgctaccttg ctcgaagcgg cggtgctgca cgatgccgcc
     1321 gacctgggcg cgcacctcgg cgctgcgcaa agccgccacg cgccccggca ggtcctcgct
     1381 gacggtcaat gcctgtgctt cgagggtcat cactgaaacc tgaagcggcg cggcgggcgc
     1441 tttgttttcc gccgccgggc cgcaagcggc caccccgagc agaagcgccc cggcaaacag
     1501 gatgcgcgca aaacctttta tggaactcat gccaaacctg acctcgtcaa ttgccttttg
     1561 ccgcccgaag gcgcgagaaa tacgaccgga gggcgagtct ggcgcacagt tgtcgaggcg
     1621 gtttcgaggc tttttggaga aagcatggag gcggcgataa gattcgccca tgcccgaatc
     1681 aagcccaacc gccgctcgcc cgcctgccgc cccggcccag gcgctcgtcc tgattgccga
     1741 ggacgaaccc gagatcgccg aaatcctcag cgcctacctg caacgcagcg ggctgcgcac
     1801 ccggcacgcg agcgatggcc agcaggccct gactatgcac cgggcgctca agcccgattt
     1861 gctgctgctc gacgtgcaga tgccgcagct cgacggctgg aaggtgctca gcgaaatccg
     1921 cacccgtggc gatacgccgg tgatcatgct gaccgcgctc gaccaggaca tcgacaagct
     1981 gatggggctg cgcctgggcg ccgacgacta cgtggtcaaa ccgttcaacc cggccgaagt
     2041 ggtggcgcgg gtcgaggcag tactgcgccg cacccgcgcg gcgcccaccg ccgcgacgcc
     2101 tatcctgcga gccggcccct tccagatcga tcccgacagc cacgaagcca gcgtctaccg
     2161 cgacggccgg caacagatgc tggatctcac gctgaccgag ttcaagctgc tcgcctgcct
     2221 gatgcgcgcg cccaaacgcg tgttcagccg cagcgaactg ctggccaact gcctgcccga
     2281 aggcgacacc caggagcgca ccgtcgacag ccacgtcagc aagctgcgca agaaactgga
     2341 ggcgctgggc atcgaaggcg tcccaaccag cgtctggggc gtcggctatc gcttcggcgg
     2401 cgacgcatga ccccctcgca cagcctgcgc cggcagatca tccagtcctt cgtggtgatg
     2461 gcgctgggca tcatcctgat ctcggtcttc ggctcctacg ccttctatgc gctggccctg
     2521 gcctatctgc cgggcagcat ttccgacacc tggataccgt cgcaggtcga aacggtgtgg
     2581 atcgtctgca ccgtcgccgc cgcgctggcg atggcggtgt tcgccgcgat ccgtctgtcg
     2641 cggcgcatcc tgacgccgct caactcggtc gcc
//
LOCUS       DAOSDX010000363         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13405_length_2672_cov_3.272831, whole genome shotgun sequence.
ACCESSION   DAOSDX010000363 DAOSDX010000000
VERSION     DAOSDX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13405_length_2672_cov_3.272831"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2468)
                     /locus_tag="PLN02_08605"
     CDS             complement(<1..2468)
                     /locus_tag="PLN02_08605"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOI52423.1"
                     /translation="MTRSFRFNLLALAIASCFGAAHANPTGGQVVSGQATMVNSGNLL
                     TVTNSANAIINWQTFSIGRNETTCFVQPSASSAVLNRVLGNNPSELLGQLQSNGRVFL
                     INPAGILVGKDAVIDVAGLVASTLNLSDSNFLAGKQKFDQTPGAAALHNAGTIRTADG
                     GQVYLVAPKVNNSGSIAAADGTVVLAAGERVEIGDTALPGLKVEVVGAGEASNVGELL
                     AASGKVGIVGALVRNSGKVDVSAIEREGGRIFLRAARKATVDGGELLATGARGGRISV
                     EGEEVDIAAGLLDASGEQAGGQVLVGGGYQGRNSEVRNATRTRFASAAEIRADATENG
                     NGGEVVVWSDDTTLAAGRISARAGTLGGDGGLVEVSGKSHLAFRAQVDTRAPAGKTGT
                     LLLDPSDVTIASRGDRAVTTVDGQFDTAYAVAGASESSAFTLYWEDLVSQLGQNNVVV
                     QTSSAASGAGNIDVVDGQTFASGNQLSLLAENNLTVHTGADVVNTGNGGFQFVAGWNP
                     TSGASAPLANNGTTTGTMLINGGLQTQGQINLSSKGDTTLNGLVKSTAGPVVLTVGGN
                     LEINLPAGAPEQYVDDDYFEYRFPSGYLFSFYGTSYDKAYISSNGLITFGSGTSAYSD
                     SQGGLAAFQMPVIAAAWNDWEINASQGESIQITRPSSNELAVKWDVGRYYRSGNKALF
                     ETVLNRQNGNITFNYGATSTESNAAVHPDFAGDVTIGLAKGDGTGQISGLMNQESFSM
                     HNLKSTTFRPGANGYTETIANSGGQLASKGAVIYSSDIGFAFGNTNIASYTTLQAATQ
                     LVLNVGGRISGYGGISTPSLAIDAG"
     gene            complement(2482..>2672)
                     /locus_tag="PLN02_08610"
     CDS             complement(2482..>2672)
                     /locus_tag="PLN02_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52424.1"
                     /translation="GRNNLLAGESQPRKSVSSYGVGMRLVLGKNLSARFDVARVIKGA
                     EARDSGDMRGHFSLMASF"
BASE COUNT          495 a          883 c          785 g          509 t
ORIGIN      
        1 ccggcatcga tggcgagcga cggcgtactg atcccgccat agccgctgat ccgcccaccg
       61 acattgagca ccagttgagt tgcggcctgc agcgtggtgt aggaggcaat attggtattg
      121 ccgaaggcga agccgatatc cgacgaatag atgaccgcgc ccttactagc caactgaccg
      181 ccactgttgg caatggtctc cgtgtagccg ttggcgcccg gccggaaggt cgtcgatttc
      241 aggttgtgca tcgagaaact ttcctggttc atcaacccgg aaatctgccc cgtaccatcc
      301 cccttcgcca agcctatggt gacgtcgcca gcaaagtccg gatggacggc agcattgctt
      361 tcagtactgg ttgccccgta gttgaaagtg atgttgccgt tctggcgatt gagcacggtt
      421 tcgaacagcg ccttgttgcc ggaacggtaa taacgcccga catcccactt gacggcgagt
      481 tcgttgctac tggggcgggt gatctggatc gactcgccct gagacgcatt gatttcccag
      541 tcattccacg ctgcggcaat caccggcatc tgaaaggcgg ccagcccgcc ttgcgaatcg
      601 ctgtaagcac tggtgccgct accgaaggtg atcaagccgt tgctggagat ataggccttg
      661 tcgtagctgg tgccataaaa gctgaacagg taaccgctcg ggaagcgata ttcgaaatag
      721 tcgtcatcaa cgtactgctc aggcgcaccg gccggcaggt tgatctccag attgccgcca
      781 accgtcaaca ccacaggccc tgccgtcgat ttcaccaagc cattgagcgt cgtgtcgccc
      841 ttcgaactca ggttgatctg accttgcgtc tgcaaaccac cattgatcaa catcgtgcct
      901 gtcgtcgttc cgttgttggc gagcggcgcg ctggctcccg aggtcgggtt ccagccagca
      961 acgaactgaa aaccgccatt ccccgtattc acgacgtcgg caccagtgtg cacggtcagg
     1021 ttgttctcgg ccagcaagct gagctggttg ccgctggcga aggtctggcc atcgacgaca
     1081 tcgatgttgc cggcaccgct cgccgctgag ctggtctgca cgacgacgtt gttctgtccc
     1141 aactggctga ccaggtcttc ccagtacaaa gtgaacgccg agctttcact ggcgccggcg
     1201 acggcatagg ccgtgtcgaa ctgaccatcg accgtcgtca ccgcccggtc accgcgactg
     1261 gcgatggtga catcgctcgg atcgagcaac agcgtaccgg tcttgccggc cggagcgcgg
     1321 gtatcgacct gcgcccggaa agccaggtgc gacttgccgg agacttcgac cagaccgcca
     1381 tcgccgccca gcgtgccggc ccgcgcggaa atccgcccag cggccagcgt ggtgtcgtca
     1441 gaccagacga cgacctcacc gccattgcca ttttcggtgg catcggcgcg gatttcggcg
     1501 gcgctggcaa agcgcgtccg ggtagcattc ctgacttcgg aattgcgccc ctgatacccc
     1561 ccgccaacca gcacctgacc gcctgcctgc tcgccggaag cgtcgagcag accggcggcg
     1621 atgtccacct cctcgccctc gacgctgatc cgcccgccgc gcgcaccagt ggccagcaac
     1681 tcaccgccat cgacggtcgc cttgcgggcg gcgcgcagga agatgcgacc gccttcacgc
     1741 tcgatcgccg agacatcgac cttgccgctg ttgcgcacca gcgcgccgac gatgcccacc
     1801 ttgccggaag cggccagcag ctcgccgaca ttgctcgcct cgcctgcgcc gacgacttcg
     1861 accttgaggc cgggcaaggc ggtatcgccg atctcgacgc gctcgccggc ggccagtacc
     1921 accgtgccat ccgccgcggc gatgctgccg ctgttgttga ccttgggcgc caccagatag
     1981 acctgtccgc catcagcggt acggatcgtc ccggcgttgt gcaatgccgc agcgcccggg
     2041 gtctggtcaa atttctgctt gccggcgagg aagttgctat cgctcagatt cagcgtcgac
     2101 gccaccaaac cggcaacgtc gatcactgca tccttgccaa ccagaatgcc ggccgggttg
     2161 atcaggaaca cccggccgtt cgactgcaac tggccaagca actctgaagg attattgccg
     2221 agcacccggt taagtacggc gctgctcgcc gacggctgga cgaagcaggt cgtctcgttg
     2281 cgcccgatcg aaaaggtctg ccagttgatg atcgcgttgg ccgaattggt cactgtcagc
     2341 aggttgccgc tattgaccat cgttgcctgc ccggaaacca cttgcccccc ggtcgggttg
     2401 gcgtgggcgg caccgaagca ggaagcgatc gccagcgcca gcagattgaa cctgaaactg
     2461 cgtgtcatga tcgtcatctc ctcagaacga tgccatcagg ctgaaatggc cgcgcatgtc
     2521 gccggaatcg cgagcctcgg cacccttgat gacgcgcgcc acgtcaaacc tggcactcag
     2581 gttcttaccc agcaccagac gcatgccgac accgtagctg ctgaccgatt tcctgggctg
     2641 ggattcgccg gccagcaggt tgttgcgccc at
//
LOCUS       DAOSDX010000364         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13409_length_2672_cov_2.708827, whole genome shotgun sequence.
ACCESSION   DAOSDX010000364 DAOSDX010000000
VERSION     DAOSDX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13409_length_2672_cov_2.708827"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /gene="glyA"
                     /locus_tag="PLN02_08615"
     CDS             complement(<1..575)
                     /gene="glyA"
                     /locus_tag="PLN02_08615"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313521.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOI52425.1"
                     /translation="MFSAKDTLAKVDPELWQAITAENQRQEDHIELIASENYVSKAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEHVDVAEQIAIDRIKALFGADAANVQPNSGSQAN
                     QAVLMAFCKPGDTIMGMSLAEGGHLTHGMPLNMSGKWFNVVSYGLNEKEEIDYDRMEA
                     LAREHKPRLIVAGASAYALRIDFERFAKIAK"
     gene            complement(613..1272)
                     /locus_tag="PLN02_08620"
     CDS             complement(613..1272)
                     /locus_tag="PLN02_08620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOI52426.1"
                     /translation="MRFDEESPRPTRLCVVRHGETEWNAERRIQGQIDIGLNATGQRQ
                     AQAAGRWLKTAGISALYASDLRRAWDTALAIGAALQLQPVATPELRERRYGVFEGLTY
                     AEAAARFPEGYAAFEGRNADYAFENGESLKVMYARVTGKLREIAALHPGQNVVVVVHG
                     GVLDVINRFVRGNPLEMARDFLIPNAGINWIATLDGRWTLESWGETGHLAGDALDELP
                     S"
     gene            complement(1274..2449)
                     /locus_tag="PLN02_08625"
     CDS             complement(1274..2449)
                     /locus_tag="PLN02_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOI52427.1"
                     /translation="MQRRDFLALAAAGAAASLAACGKDETPPPVVEPAPPKKSVAPPS
                     EAAPKPVEAAAVQSAYPAVRWRLTSSFPRSLDTIFGGAEVLADRLRAMTGGRFDIRVF
                     QGGEIVPGLQALDAVEQGTVEMAHTCSYYYVGKDKSFGFGTSMPFGMNARQMNAWVYY
                     GGGQELLDDFYGNFNVLSFAGGNTGTQMGGWWRKKVSAVDDLKGIKMRIAGLGGAVFS
                     ELGVVPQQIAGGDIYPALERGTIDAAEWVGPYDDERLGFYKVAKNYYYPGWWEPGPMI
                     HFFVNRNEWEKLPREYQEAFRAAAFEANVTMMARYDHQNPIALKKLLQSGVKLQEFSR
                     PILEAAYKAAQQLYADEAKKNPAFKKILKAYDKYRRSENAWFSVAENRMDDFLQSQA"
BASE COUNT          444 a          893 c          827 g          508 t
ORIGIN      
        1 tccttggcga tcttggcgaa gcgctcgaaa tcgatgcgca gcgcgtaggc cgaggcaccg
       61 gcgacgatca ggcgcggctt gtgttcgcgg gccagcgctt ccatcctgtc gtagtcgatc
      121 tcttcctttt cgttcaggcc gtaagaaacg acgttgaacc acttgccgga catgttgagc
      181 ggcatgccgt gggtcaggtg gccgccttcg gccaggctca tgcccatgat cgtgtcgccc
      241 ggcttgcaga aggccatcag cacagcctgg ttggcctgcg aaccggagtt cggctggacg
      301 ttggcggcat cggcgccgaa cagcgccttg atgcggtcga tggcgatctg ctcggcaacg
      361 tcaacgtgtt cgcaaccgcc gtagtagcgc ttgcccgggt agccttcggc gtacttgttg
      421 gtcagctgcg agccctgggc ttccatgacc gccttgctga cgtagttctc ggatgcgatc
      481 agttcgatgt gatcttcctg acgctggttt tcggccgtga tggcctgcca cagttcaggg
      541 tcaactttcg ccagggtgtc tttcgcggaa aacatgggga tgcctcaagc cgttgaaaag
      601 gccgcgattt tatcacgaag gcagttcgtc caacgcgtcg cccgccagat gcccggtttc
      661 gccccagctt tccagcgtcc accggccatc gagcgtggcg atccagttga tgccggcgtt
      721 cggaatcagg aagtcgcgcg ccatctcgag cggattgccg cgcacgaagc ggttgatcac
      781 atcaagcacg ccgccatgga cgacgacgac gacattctgg ccgggatgca gggcagcgat
      841 ttcgcgcagc ttgccggtaa cgcgggcgta catgactttc aggctctcgc cgttctcgaa
      901 cgcgtaatcg gcgttgcgcc cctcgaacgc ggcatagccc tcgggaaagc gcgccgccgc
      961 ctcggcatag gtcaggcctt cgaagacgcc gtagcggcgt tcgcgcaact cgggcgtcgc
     1021 taccggctgc aactgcagcg cggcgccgat cgccagcgcc gtatcccagg cccggcgcag
     1081 gtcgctggcg taaagcgcgc tgattccagc ggtcttcagc cagcgcccgg cggcctgcgc
     1141 ctgacgctgg ccggtcgcat tgaggccgat gtcgatctgc ccctggatgc gccgttcggc
     1201 attccactcg gtctcgccgt ggcggaccac gcaaagccgc gtcgggcgcg gcgattcttc
     1261 atcaaaacgc atctcaggcc tgcgactgga ggaaatcgtc catacggttc tcggcaacgc
     1321 tgaaccaggc gttctcgcta cgccggtact tgtcgtaggc cttgagaatc ttcttgaatg
     1381 ccgggttctt cttcgcctcg tcggcgtaaa gctgctgcgc cgccttgtac gccgcttcga
     1441 gaatcggccg cgagaactcc tgcagcttga cgccgctctg cagcagcttc ttcagcgcaa
     1501 tcgggttctg gtggtcgtag cgcgccatca tcgtcacgtt ggcttcgaaa gccgccgccc
     1561 ggaacgcctc ctggtattcg cgcggcagct tctcccactc gttgcgattg acgaagaaat
     1621 ggatcatcgg ccccggctcc caccagcccg ggtagtagta gttcttcgcc accttgtaga
     1681 aacccaggcg ctcgtcgtcg tagggaccga cccattcggc ggcgtcgatg gtgccgcgct
     1741 cgagtgccgg gtagatgtcg ccgccggcga tctgctgcgg gacgacgccg agttcggaaa
     1801 agaccgcgcc gccgaggccg gcgatgcgca tcttgatccc cttgaggtcg tcgaccgcgc
     1861 tcaccttctt ccgccaccag ccgcccatct gcgtcccggt gttgccaccg gcgaaggaga
     1921 ggacgttgaa attgccgtag aaatcgtcga gcagttcctg gccgccaccg taatagaccc
     1981 aggcgttcat ctgccgcgcg ttcatgccaa agggcatcga ggtgccgaaa ccgaagctct
     2041 tgtccttgcc gacgtaatag taggaacagg tatgcgccat ctcgaccgtg ccctgctcga
     2101 ccgcatcgag cgcctgcagg ccgggaacga tctcgccgcc ctggaagacg cggatgtcga
     2161 aacggccgcc ggtcatcgcc cgcagccggt cggcgagaac ctcggcgcca ccgaagatgg
     2221 tgtccagact gcgcggaaaa ctcgaagtca ggcgccagcg caccgccggg taggcactct
     2281 gcacggcggc tgcctcgacc ggcttcggcg ccgcctcgct gggcggggcc accgacttct
     2341 tcggcggcgc cggctcaacg accggcggcg gcgtttcatc cttgccgcaa gcggcaagcg
     2401 aagccgctgc gccagccgcc gccaaggcca gaaaatctct acgttgcatg cctgtctcct
     2461 cccgctcggg cgggattttg ctgtatccaa tggcatgcat tcttccgtaa agcgcgcgac
     2521 ttggcgagca aaaaccgaaa ttcgcccccc gccgccaacc gtcgtcgtaa actcggggag
     2581 cgccgccgtt ccggaaaact caacgtgtcc caactgcccg atcgcctgca caagcttctt
     2641 gccgccgccg gctcgcccgc cgggctgagc cg
//
LOCUS       DAOSDX010000365         2671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13413_length_2671_cov_2.696101, whole genome shotgun sequence.
ACCESSION   DAOSDX010000365 DAOSDX010000000
VERSION     DAOSDX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13413_length_2671_cov_2.696101"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLN02_08630"
     CDS             <1..427
                     /locus_tag="PLN02_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOI52428.1"
                     /translation="VRRALAAGDMAHAAKLLGRPYMMDGQVVHGQKLGRQLGFATANL
                     RIKHNPLPMTGVFAVEVGGLGETPLPGVANLGIRPTLGGTRPLLEVHLFDFDRDIYGA
                     HISVRFVHKLRDEQRFPNFDALKAQIAADAAAARAFFKR"
     gene            436..>2671
                     /gene="ileS"
                     /locus_tag="PLN02_08635"
     CDS             436..>2671
                     /gene="ileS"
                     /locus_tag="PLN02_08635"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOI52429.1"
                     /translation="MADYKDTLNLPDTPFPMRGDLPKREPKWVARWQEKQLYRRIREI
                     SAGRPKFILHDGPPYANGSLHLGHALNKILKDIIVRSKTLAGFDAPYVPGWDCHGLPI
                     EHKVEVTHGKNLPADKVRELCRAYAAEQIEIQKAGFIRLGVLGDWDNPYLTMNFANEA
                     GEIRALAEIVRKGYVFKGLKPVNWCFDCGSALAEAEVEYADKKSPTIDVAFPVDAADG
                     QKLAAAFGQPALTKPAFAVIWTTTPWTIPANQALNIHPEHDYALVDTGERLLVLAAEL
                     LEASLQRYDLQGSVLATVQGKALAGLRFRHPLYDRESPVYLADYVGLDAGTGVVHSAP
                     AHGVDDFNTWLAYGRGNDEILSIVKGDGVYVDDLPFFGGMSIWKANQAVCDKLTEVGC
                     LLSHGNISHSYMHCWRHKTPVIYRATAQWFVGMDRVASQPAQAGLGGEGETLREIALR
                     GVEATEFYPGWGKPRLHNMIANRPDWCISRQRNWGVPIPFFMHRETGELHPRTVELME
                     EVAKRVEKEGIEAWFKLDAAELLGAEANQYDKISDTLDVWFDSGTTHWHVLRGSHNDG
                     HASGPRADLYLEGSDQHRGWFHSSLLTGAAIDGHPPYKALLTHGFTVDQQGRKMSKSL
                     GNTILPEEVTDKMGADILRLWVASTDYSGELAMSKEILARVVEGYRRIRNTLRFLLAN
                     ISDFDASTAMLPVDQWLEIDRYALALARRLQEQVTADYGRYEFHRVVQALQGFCSEEL
                     GAFYL"
BASE COUNT          495 a          861 c          849 g          466 t
ORIGIN      
        1 cgtgcgccgg gcgctggctg ctggcgacat ggcgcacgcg gcgaagttgc tcggccgccc
       61 gtacatgatg gacggccagg tggttcacgg ccagaagctc ggccgccagc tcggtttcgc
      121 cacggccaac ctgcgcatca agcacaaccc gctgccgatg accggcgttt ttgcggtcga
      181 ggtcggcggc ctcggcgaga cgccgctgcc cggcgttgcc aacctcggca tccggccgac
      241 gctcggtggc acgcgaccgc tgctcgaagt ccatctgttc gatttcgatc gcgacatcta
      301 cggcgcgcat atttcggttc gtttcgtgca taaactgcgc gacgagcaac gttttcccaa
      361 tttcgatgcc ctcaaggcgc agatcgcggc cgatgccgcg gcggcgcggg ccttcttcaa
      421 gcggtgagca agcaaatggc tgattacaaa gacaccctga atcttcccga taccccgttc
      481 ccgatgcgcg gcgacctgcc caagcgcgag ccgaagtggg tggcacgctg gcaagagaag
      541 cagctttacc ggcggattcg cgagatttct gccgggcggc cgaagttcat cctgcacgac
      601 ggcccgccgt atgccaacgg cagcctgcat ctgggccacg cgctgaacaa gattctcaag
      661 gacatcatcg tccgttcgaa gacgctggcc ggcttcgatg cgccttacgt gcccggctgg
      721 gattgccacg gtctgccgat cgagcacaag gtcgaggtga cgcacggcaa gaacctgccc
      781 gccgacaagg tgcgcgaact atgccgcgcc tacgcggccg aacagatcga aatccagaag
      841 gccggtttca tccggctcgg cgtgctgggc gactgggaca atccctacct cacgatgaac
      901 tttgccaacg aagccggcga aatccgggcg ctggccgaaa tcgtcaggaa gggctatgta
      961 ttcaagggcc tgaagccggt aaattggtgt ttcgattgcg gttcggcgct ggccgaggcg
     1021 gaagtcgagt atgccgacaa gaaatcgccg accatcgacg tggcctttcc ggtcgacgcc
     1081 gccgacgggc aaaaactggc ggctgccttc ggccagccgg cgctgacgaa accggcgttc
     1141 gcggtgatct ggacgacgac gccgtggacc attccggcca accaggcgct gaacatccat
     1201 cccgaacatg actatgcgct ggtcgatacc ggcgagcggc tgctggtgct ggccgccgaa
     1261 ctgcttgaag cctcgctgca acgctacgac ctgcaaggca gcgtgttggc caccgtgcag
     1321 ggcaaggcgc tggcagggct gcgcttccgc cacccgctgt acgatcgcga gtcgccggtc
     1381 tatctcgccg actacgtcgg gctggatgcc ggtaccggcg tcgtgcatag cgcgccggcg
     1441 cacggcgtcg atgacttcaa tacctggctc gcttacgggc gcggcaacga cgagattctt
     1501 tccatcgtca agggcgatgg cgtttatgta gacgacctgc ctttctttgg cggcatgtcg
     1561 atctggaagg ccaatcaggc ggtctgcgac aagctgaccg aagtcggctg cctgctctcg
     1621 cacggcaata tcagccacag ctacatgcac tgctggcggc acaagacacc ggtgatctac
     1681 cgcgccacgg cgcagtggtt cgtcggcatg gacagggtgg ccagccaacc ggcacaagcc
     1741 gggctcggcg gagaagggga gaccttgcgc gagatcgctt tgcgcggcgt cgaggcgacc
     1801 gagttctatc ccggctgggg caagccgcgc ctgcacaaca tgatcgccaa ccggccggac
     1861 tggtgcatct cgcgccagcg caactggggg gtgccgattc ccttcttcat gcaccgggaa
     1921 accggcgaac tgcatccgcg taccgtcgaa ctgatggaag aagtggccaa gcgcgtggaa
     1981 aaggaaggca tcgaggcctg gttcaagctc gacgccgccg aactgctcgg tgccgaggcc
     2041 aaccagtacg acaagatcag cgataccctc gacgtctggt tcgactccgg cacgacgcac
     2101 tggcatgtgc tgcgcggttc gcacaacgac ggccatgcca gcggtccgcg cgccgacctc
     2161 tacctggaag gctccgacca gcatcgcggc tggttccatt cgtcgctgct taccggcgcg
     2221 gcgattgacg gtcatccgcc gtacaaggcg ctgctgacgc acggtttcac cgtcgaccag
     2281 caggggcgca agatgagcaa gtcgctcggc aacaccattc tgccggaaga agtcaccgac
     2341 aagatgggcg ccgacatcct gcgcctttgg gttgcgtcga ccgattattc gggcgaactg
     2401 gcgatgtcca aggaaatcct ggcgcgtgtc gtcgaaggct accgccgcat ccgcaacacg
     2461 ctgcgcttcc tgctcgccaa tatttccgat ttcgacgcgt cgaccgcaat gctgccagtc
     2521 gatcagtggc tggagatcga ccgctacgcc ctggcgctgg cgcgccgcct gcaggagcag
     2581 gtgacggccg actacggccg ctacgagttc catcgcgtcg tccaggcgct gcagggattt
     2641 tgttccgagg aactgggcgc cttctacctc g
//
LOCUS       DAOSDX010000366         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13441_length_2666_cov_2.553811, whole genome shotgun sequence.
ACCESSION   DAOSDX010000366 DAOSDX010000000
VERSION     DAOSDX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13441_length_2666_cov_2.553811"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLN02_08640"
     CDS             <1..467
                     /locus_tag="PLN02_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI52430.1"
                     /translation="AIDGILLQVPVLGSTLMAAAMAQAAWTFGLLLKSRLTVLEALRS
                     VAQVSANARISHALEAACEQVIEGRALTVALDRPPIPPLVRHMAAVGERSGEMEAVME
                     ALGNHYQKELDARVKMLSALIEPVLTLFIGGVVGFVYYAFFQAVLAVSTGGG"
     gene            474..995
                     /locus_tag="PLN02_08645"
     CDS             474..995
                     /locus_tag="PLN02_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52431.1"
                     /translation="MNTHRLPALALLALLTLSGDLFAQNRALDLAADRNADLSPTMRE
                     NLATIGRYADTVNRRLAPSEPKPRRLDQVADPFEVSPQLRDGPKNNSSFGGLPGAGRL
                     ELQRQVQLRALLAIGGRRVAQLLIRDKDSLTVHDRELLDLGELGVFEVRIERDAVSLF
                     NPSTPQGTKLILR"
     gene            992..>2666
                     /locus_tag="PLN02_08650"
     CDS             992..>2666
                     /locus_tag="PLN02_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52432.1"
                     /translation="MKRFLSALCFLLLAVAPLAAAPAKAPARPAAGVIGDIQLTRVRL
                     EDVARLLSQVGQANVVVTSEVFDKQVSIYLRNARVEDMVRNVCRAAGVWYRKDADADT
                     YIIMSAAEYQKDLAIVRDEKTRVFTLRHHNVVATANAIRALFGSRVALAAPVEEMPPV
                     SLGAGNRQKAGSRNTGNGSGNRSGNNGNNSGTAAGTADNGDFAASGSSGGLQGGSRGG
                     GASAVVSDPRAELDRLSAERLSTQVRVDEQGQLLAGAGSVQEVSAHLGPPINVTYNRL
                     NNLLMVRSGDLAALEQIGQLIAEMDRPPKQVLLEMKILEVTLDDGYRSVFDIGLAGKG
                     TTAGPGGWASQSNQGTGISARNSLGSGLFDVENDANLIWQVMSSSLTMRLQLLANENK
                     LKVLSSPMLVAANNQQARLFIGDERVLTVGASSQSVTGTTGATNTTITVETEKRDVGQ
                     TLAILPRINGDRTISLTIDQDSSTVKIGDATIPITTANGNVIYFPIDTVNTANLQVTA
                     HAKDGMTVAIGGMIRESSQRDEEKVPLLGDIPGVGFFFKRDVRAAVRSQI"
BASE COUNT          481 a          992 c          825 g          368 t
ORIGIN      
        1 gcgccatcga cggcatcctg ctgcaggtgc cggtgctcgg cagcacgctg atggcggcgg
       61 cgatggcgca ggccgcatgg actttcggcc tgctcctgaa aagccgcctg accgtgcttg
      121 aagccttgcg ctcggtcgcc caggtcagcg ccaacgccag gatcagccac gccctcgaag
      181 ccgcctgcga acaggtgatc gaaggccgcg ccctgaccgt cgccctcgac cgcccgccga
      241 tcccgccgct ggtccgccac atggcggccg tcggcgagcg cagcggcgag atggaagcgg
      301 tgatggaagc gctcggcaac cactaccaga aagagctcga cgcccgcgtc aagatgctat
      361 cggcactgat cgaaccggtg ctgacgctgt tcatcggcgg ggtcgtcggc tttgtctatt
      421 acgccttctt ccaggccgta ctcgccgtgt ccaccggcgg gggttaggag ttcatgaaca
      481 cgcaccgcct tcccgccctg gccttgctcg ccctgctgac gctgagcggc gacctgttcg
      541 cccagaaccg ggcgctcgac ctggccgccg accgcaatgc ggacctgagc ccgacaatgc
      601 gcgagaacct cgccaccatc ggccgctacg ccgacacggt caatcgccgg ctggccccga
      661 gcgagcccaa accgcgccgc ctcgaccagg tcgccgaccc gttcgaagtc tcgccgcaac
      721 tgcgcgacgg cccgaagaac aacagcagct tcggcggcct gcccggcgcc ggccgcctcg
      781 aactgcaacg ccaggtccag ttgcgggcct tgctggccat cggcggccgg cgcgtcgccc
      841 agctcctcat ccgcgacaag gacagcctca ccgtgcatga ccgcgaactg ctcgacctcg
      901 gcgagctggg cgtcttcgaa gtccgcatcg aacgcgacgc ggtctccctg ttcaacccca
      961 gcacgccgca aggcaccaag ttgatcctgc gatgaaacgc ttcctgtccg ccctctgctt
     1021 cctccttctc gccgtcgctc cgctcgccgc cgcgccggcc aaggcgccgg cccggcccgc
     1081 tgccggcgtc atcggcgaca tccagctgac ccgggtccgc ctcgaagacg tcgcccgcct
     1141 gctgtcgcag gtcggccagg ccaacgtcgt cgtcaccagc gaggtgttcg acaagcaggt
     1201 ctcgatctac ctgcgcaacg cccgcgtcga ggacatggtg cgcaacgtct gccgcgccgc
     1261 cggcgtctgg taccgcaagg acgccgatgc cgacacctac atcatcatga gcgcggcgga
     1321 ataccagaag gatctggcca tcgtccgcga cgagaagacg cgggttttca ccttgcgcca
     1381 ccacaacgtc gtcgccaccg ccaacgccat ccgcgccctg ttcggcagcc gcgtcgcgct
     1441 ggccgccccg gtcgaggaaa tgccgccggt ttccctcggt gccggcaacc ggcagaaggc
     1501 cggcagccgc aacacgggca acggtagcgg caatcgcagt ggcaacaacg gcaacaacag
     1561 cggcacggcg gccggcaccg ccgacaacgg cgattttgca gccagcggca gcagcggtgg
     1621 cctgcagggc ggtagccgcg gcggcggggc ttctgccgtg gtcagcgacc cgcgcgccga
     1681 actcgaccgc ctgagcgccg agcgcctatc gacgcaggtc cgcgtcgacg aacagggaca
     1741 actgcttgcc ggcgccggca gcgtgcagga agtctcggcc cacctcgggc cgccgatcaa
     1801 cgtcacctac aaccgcctga acaacctgtt gatggtgcgc agcggcgacc tcgccgcgct
     1861 ggaacaaatc ggccagttga tcgccgagat ggaccggccg ccgaaacagg tgctgctgga
     1921 aatgaagatc ctcgaagtga cgctcgatga cggctaccgc tcggtcttcg acatcggcct
     1981 cgccggcaag gggacgaccg ccggccccgg cggctgggcc agccagtcca accagggcac
     2041 cggcatcagc gcgcgcaaca gcctcggctc cgggctgttc gacgtggaaa acgacgccaa
     2101 cctgatctgg caggtgatga gcagctcgct gaccatgcgc ctgcaactgc tggccaacga
     2161 gaacaagctc aaggtcctct cctcgccgat gctggtcgcc gccaacaacc agcaggcgcg
     2221 gctgttcatc ggcgacgaac gcgtcctcac cgtcggcgct tcgtcgcaga gcgtcaccgg
     2281 gacaaccggc gcgaccaaca cgacgatcac cgtcgaaacc gaaaagcgag acgtcggcca
     2341 gaccctggcc atcctgccgc gcatcaacgg cgaccggacg atcagcctga ccatcgacca
     2401 ggacagctcg acggtcaaga tcggcgacgc gacgatcccg atcaccaccg ccaacggcaa
     2461 cgtcatctac ttcccgatcg ataccgtcaa caccgccaac ctgcaggtca ccgcccacgc
     2521 caaggacggc atgaccgtcg ccatcggcgg catgatccgc gaaagcagcc agcgcgacga
     2581 ggaaaaggtg ccgctgctcg gcgacatccc cggcgtcggc ttcttcttca agcgcgacgt
     2641 gcgggcggcg gtacgctcgc agatcg
//
LOCUS       DAOSDX010000367         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13471_length_2662_cov_2.762946, whole genome shotgun sequence.
ACCESSION   DAOSDX010000367 DAOSDX010000000
VERSION     DAOSDX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13471_length_2662_cov_2.762946"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..376
                     /locus_tag="PLN02_08655"
     CDS             170..376
                     /locus_tag="PLN02_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52433.1"
                     /translation="MLPPRLRPKNKLPLRLLPRLLTLPLPRLLPRLTLLPLRLLPRLP
                     TLPLLLSLTLPRLPLPKKPRSNLA"
     gene            complement(463..1602)
                     /gene="bamC"
                     /locus_tag="PLN02_08660"
     CDS             complement(463..1602)
                     /gene="bamC"
                     /locus_tag="PLN02_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HOI52434.1"
                     /translation="MNHRISGLTLCLVAVAVAGCSGNIIPESRKIEYKSAGKVPTLEV
                     PPDLTQLSRDDRYLVPDDAGKGSATFSAYAAERTPEAQAGQSAVLPKVDKVRVERSGS
                     QRWLVVNSAPEALWDPIKEFWQETGFIVNIERPEAGVMETDWAENRAKIPSDIIRNTL
                     GRIVDSLYSSGERDKFRTRLEPGAQPGTTEIYISHRAMEEVYTSSAKEDTRWQPRAAD
                     PDLEAEMLRRLMVRLGAEEKRAEAALATPKSEPRARLTRADDGSGKLEVFDRFDRAWR
                     RVGLALDRVGFTVEDRDRSRGIYFVRYVDPEQDNASKKSDGLLSRLAFWKSDEKSAVS
                     REQYRIHVEDGGGSSSSVQVLTSEGGTDKSETAKKILALLQQQLQ"
     gene            complement(1620..2498)
                     /gene="dapA"
                     /locus_tag="PLN02_08665"
     CDS             complement(1620..2498)
                     /gene="dapA"
                     /locus_tag="PLN02_08665"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOI52435.1"
                     /translation="MITGSIVAIVTPMHEDGSLDFNALRNLVDFHVQEGSDGIVIVGT
                     TGESPTVDVDEHCELIRVTVEHAAGRIPVIAGTGANSTAEAIELTRFAKKAGADMALS
                     VVPYYNKPSQEGLYRHFKAIAEAVDLPVLLYNVPGRTVADMSNDTILRLAEVPGIVGV
                     KDATGNLDRACDLIQRAPEGFALYSGDDLSCVLSIMLGFHGNISVTANVAPRLMHEMC
                     VAAAAGDVARAKEIHFRLLGLHRDLFCEANPIPVKWAVSRMGLMPEGIRLPLTPLAEA
                     GQARVLAAMRQAGIDV"
BASE COUNT          456 a          845 c          839 g          522 t
ORIGIN      
        1 aacatttttt ttgcgctata attttcggcg gttcggtgta aaccggccga acaaatctat
       61 tcggatttat ttgcttcttg tcgataagga aatctcatga acaagtccat tctcgccgcc
      121 gccctgtttg ccctcgccct gaccgcctgc ggtcagaagg aagaaccggt tgctgccgcc
      181 ccggctccgg cccaagaaca agctgccgct ccggctgctg ccccggctgc tgacgctgcc
      241 gctgccccgg ctgctgcccc ggctgacgct gctgccgctc cggctgctgc cccggctgcc
      301 gacgctgccg ctgctgctgt cgttgacgct gccaaggctg ccgttaccga agaagccaag
      361 aagtaatctg gcttgaccag gcaataaaaa aacggggctt cggccccgtt tttttattgc
      421 cttggcgccg tgatccggtc acggcgccaa tacctgctgt ccttactgca actgctgctg
      481 caacagcgca agaatcttct ttgccgtctc cgacttgtcg gtaccgcctt cgctggtcag
      541 gacctgcacg ctggaactgc tgccaccgcc gtcctcgacg tgaatacggt actgctcgcg
      601 agaaaccgcc gacttctcgt cgctcttcca gaacgccagg cgcgacagca ggccatcact
      661 cttcttgctg gcattgtcct gttcaggatc gacgtagcgg acgaaataga tgccgcgcga
      721 gcgatcacga tcctcgacag tgaacccgac gcggtcaagc gccagaccga cacgacgcca
      781 ggcgcggtcg aaacgatcga agacttcaag cttgccgctg ccgtcgtcag cccgcgtcag
      841 acgcgcccgc ggctcgctct tgggcgttgc cagtgcggct tcggcgcgct tctcctcggc
      901 gccgaggcgc accatcaggc ggcgcagcat ttccgcttcc aggtccggat cggcggcacg
      961 cggctgccaa cgggtgtctt ccttggccga agaggtatag acctcctcca tcgcccggtg
     1021 gctgatgtag atttcggtgg tgcccggctg cgcccccggc tccaggcggg tgcggaactt
     1081 gtcgcgctcg ccgctggaat agagggaatc gacgatacga cccagggtgt tgcggatgat
     1141 gtcggacgga atcttggcgc gattttccgc ccagtcggtt tccatcacgc cggcttccgg
     1201 tcgttcgatg ttgacgatga aaccggtttc ctgccagaac tccttgatcg ggtcccacaa
     1261 ggcctccggt gccgaattga cgaccagcca gcgctggctg ccggaacgct cgacgcgcac
     1321 cttgtccacc ttgggcagca ccgccgattg cccagcctgc gcttccggcg tccgttcagc
     1381 ggcataggcc gagaacgtgg ccgagccctt gccggcgtcg tccggcacca ggtagcggtc
     1441 gtcgcggctc aactgggtga gatccggcgg cacttcgagg gtaggcacct tgcccgccga
     1501 cttgtattcg atcttgcgcg actcgggaat gatgttgcca ctgcagccgg ccacggcaac
     1561 cgcgacgaga cagagggtga ggccggaaat ccggtgattc atggtcttca tgctttcttt
     1621 catacgtcga taccggcctg gcgcatggct gccagcacgc gtgcctgacc ggcttccgcc
     1681 agcggcgtca gcggcaggcg aatgccttcg ggcatcaggc ccatgcggct gaccgcccac
     1741 ttgacgggaa tcgggttggc ctcgcagaag aggtcacgat gcaggccaag caggcggaaa
     1801 tggatttcct tggcgcgcgc gacatcgccg gcggcggcgg cgacgcacat ttcgtgcatc
     1861 aggcgcggcg cgacgttggc ggtgaccgag atattgccgt ggaagccgag catgatcgac
     1921 agcacgcagc tcaggtcgtc gccgctgtac agggcgaaac cttccggcgc acgctgaatc
     1981 aggtcacagg cgcgatccag gttgccggtc gcgtccttga cgccgacgat accgggcacc
     2041 tcggcgagac gcaggatggt gtcgttgctc atgtcggcaa cggtacggcc gggcacgttg
     2101 tagagcagga ccggtaggtc gactgcttcg gcgatcgcct tgaagtggcg gtagaggcct
     2161 tcctggctcg gcttgttgta atacggcaca accgacagcg ccatgtcggc gcccgccttc
     2221 ttggcgaaac gcgtcagttc gatggcttcc gccgtcgaat tggcgccggt gccggcgatc
     2281 accgggatgc ggccggcggc gtgttcgacc gtcacgcgga tcagttcgca gtgctcgtcg
     2341 acgtcgacgg tcggggattc gccggtggta ccgacaatga cgatgccgtc gctaccttcc
     2401 tggacgtgga agtcgaccag gttgcgcagg gcgttgaaat cgaggctgcc atcctcgtgc
     2461 atgggcgtga cgatggcgac aatggatccg gtaatcatgg cctgtttaca aataaataat
     2521 ccggtgattg taactgaagg cttccgacag gggaaaggca ggcggtaagc aattgttatc
     2581 cacccggcaa ccagcgcagc cgggcccggc tcagacgcat ccgcccggcc ggcagttgca
     2641 gttcgggatg acggtcgggt gc
//
LOCUS       DAOSDX010000368         2661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13476_length_2661_cov_3.874520, whole genome shotgun sequence.
ACCESSION   DAOSDX010000368 DAOSDX010000000
VERSION     DAOSDX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13476_length_2661_cov_3.874520"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /gene="glnE"
                     /locus_tag="PLN02_08670"
     CDS             complement(<1..1284)
                     /gene="glnE"
                     /locus_tag="PLN02_08670"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOI52436.1"
                     /translation="MNSLPDPRWQAALAHSRYLNNLFQARPALLPELAASWLQPLDEA
                     LLSAPLAQDFADDEALKSALRRLRQRAMAHIALRDLCGLAPLAEVVESMTLLADVTTN
                     FALAHYHRQLAASYGEPLDSAGRPQRLLVIGMGKLGGRELNVSSDVDYIFIYPEDGDT
                     AGPKSIENYDFFTRLGKRVIQALGDITADGQVFRVDMRLRPNGDSGPLVCSLDSLENY
                     FITQGREWERYAWIKGRTMNTGANLQPEWVEAMQKIGRPFVFRKYLDFGAINAMRDLH
                     AQIRREVARKDMADHIKLGPGGIREIEFMAQVFQLIRGGRDPALQIRPTLAVLALLVE
                     RQLLPADSERELREAYIFLRRLEHRLQYVDDAQTHMLPVDPAERQKIARSMDFADWPA
                     LLEVLNGHRGRVSRHFEAVFSDPEAGEHPRTGLWQG"
     gene            1386..>2661
                     /locus_tag="PLN02_08675"
     CDS             1386..>2661
                     /locus_tag="PLN02_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02099 family protein"
                     /protein_id="HOI52437.1"
                     /translation="MNNPSGQTETTDARLIHQGLYYRLHGLRALLGRPWVASVLRVAG
                     WLLVVGWLFFVALVLLLRFAVLPSIADYRADIERLAGKAVGQEVRIGQIAAHWRGLNP
                     ELVLDGVSLLAADGAPSFSLQRVDAVLSWHSLWRLRPVLALLSFDHPVLHVRRASDGR
                     ISVAGIESTGDSDPAFAEWVLEQKRIRIHDATIVWEDALRAAPPLILEDLQFGLDNRG
                     QRHRFGLSAVPPAELAARIDLRGEINGDLHDALERYAGNLYLELEYADLAGWQPWFDY
                     PVDLPRGRGGVRLWGEIKEGESRLTADLALQDLRIRLSRKLPALDLDRLRGRLTATYG
                     KAAWSLAARQLELQTSEGIRLAPTDFQLDWRQGESAINGNFRANQLDLEILSRLAGYP
                     PLDARSRELLQAYAPQGRISELRTSWGLQGEKL"
BASE COUNT          409 a          870 c          927 g          455 t
ORIGIN      
        1 gccctgccag agaccggtcc gcggatgttc gccggcttcc gggtcggaga agacggcctc
       61 gaagtggcgg ctgacccggc cgcggtggcc gttgagcacc tcgagcagcg ccggccagtc
      121 ggcgaagtcc atgctgcggg cgatcttctg ccgctccgcc ggatcgaccg gcagcatgtg
      181 cgtctgcgcg tcatcgacat actgcagacg gtgttcgagc cggcgcagga agatgtaggc
      241 ctcgcgcagc tcgcgctcgc tgtcggccgg cagcaactgg cgttcgacga gcagggcgag
      301 taccgccagg gtcggccgga tctgcagcgc cgggtcgcgg ccgccgcgga tcaactggaa
      361 cacctgggcc atgaactcga tttcgcgaat gccgccgggg ccgagcttga tgtggtcggc
      421 catgtccttg cgcgcgactt cgcggcggat ctgcgcgtgc aggtcgcgca tcgcgttgat
      481 cgcgccgaaa tcgaggtact tgcggaagac gaagggacgg ccgatcttct gcatcgcctc
      541 gacccattcc ggctgcaggt tggcgccggt gttcatcgtc cgccccttga tccaggcgta
      601 gcgttcccac tcgcggccct gggtgatgaa gtagttttcc agcgagtcga gcgagcagac
      661 cagcggcccg gagtcgccgt tcgggcgcag ccgcatgtcg acgcggaaga cctggccgtc
      721 ggcggtgatg tcgccgagcg cctggatcac gcgcttgccg aggcgggtga agaaatcgta
      781 gttctcgatc gatttcgggc cggcggtgtc gccgtcctcg gggtagatga agatgtaatc
      841 gacgtcggag gaaacgttca gttcgcgccc gccgagcttg cccatgccga tcaccagcag
      901 gcgttgcggc cggcccgcgc tgtcgagcgg ttcgccgtag ctggcggcga gctggcggtg
      961 gtagtgggcg agggcgaagt tggtggtgac gtcggcaagc agggtcatgc tctcgacgac
     1021 ttcggcgagc ggcgccagtc cgcacaagtc gcgcagcgcg atgtgcgcca tcgcccgctg
     1081 acgcagccga cgcaatgccg acttgagcgc ctcgtcgtcg gcgaaatcct gggccagcgg
     1141 ggcgctcagc agggcttcgt cgagcggttg cagccagctt gccgcgagtt cgggaagcag
     1201 cgccggacgc gcctggaaga ggttgttcag gtagcggctg tgggccaggg cggcctgcca
     1261 gcggggatcg gggagggaat tcatggggtt ctgtcggcaa ctgggcgaaa aggtagaata
     1321 cgccctcaaa taaattttcc cgattgtagt gacctccgac cgcatctggc aaagcgacgc
     1381 gacgtttgaa taatccgtcc ggccaaaccg aaaccactga cgcccgcctg atccaccagg
     1441 ggctgtacta ccggctgcac ggcctgcgcg ccttgctcgg ccggccttgg gtcgcgtctg
     1501 tgctgcgcgt cgccggctgg ttgctggtcg tcggctggtt gttcttcgtg gcgctggtcc
     1561 tgctgctgcg ctttgccgtg ctgccgtcga ttgccgacta tcgggccgac atcgagcgcc
     1621 tggcgggcaa ggcggtgggc caggaagtgc gcattggcca aatcgccgcc cactggcgcg
     1681 gcctcaatcc ggaactggtg ctcgacggcg tctcgttgct ggcggccgac ggtgcgccga
     1741 gcttctcgct gcaacgggtc gacgccgtgc tgtcctggca cagcctgtgg cgactgcgcc
     1801 cggtgctggc gctgctttcc ttcgatcacc cggtgttgca cgtgcgccgt gccagcgacg
     1861 ggcggatcag cgtcgccggc atcgaatcga ccggcgacag cgatccagcc tttgccgaat
     1921 gggttctgga acagaagcgc atccgcatcc acgacgcgac catcgtctgg gaagacgcct
     1981 tgcgcgccgc gccgccactg atcctggaag acctccagtt cggcctcgac aaccgcggcc
     2041 agcgtcatcg cttcggcctg tcggcggtgc cgcctgccga actggcagcg cgcatcgacc
     2101 tgcgcggcga gatcaacggc gacctgcacg acgcgcttga gcgttacgcc ggcaatctct
     2161 acctcgaact cgaatacgcc gatctggccg gctggcagcc ctggttcgac tacccggtcg
     2221 acctgccgcg cgggcgcggc ggcgtgcgcc tgtggggcga gatcaaggaa ggggaaagcc
     2281 ggctgaccgc cgacctggcg ttgcaggatc tacgcatccg cctcagtcgc aagctgccgg
     2341 cgctcgacct cgaccgtctg cgcggccggc tcaccgccac ctacggcaag gcggcctggt
     2401 cgctcgccgc ccgccaactg gagttgcaga ccagcgaggg catccggctg gcgccaacgg
     2461 atttccagct cgactggcgg cagggtgaat cggcgatcaa cggtaatttc cgggcgaatc
     2521 agctcgacct cgaaatcctc tcccggctcg ccggctaccc gccgctcgat gcgcgctccc
     2581 gcgaactgct gcaggcctac gcgccgcagg ggcggatcag cgaactacgc accagttggg
     2641 gcctgcaggg tgaaaaactg g
//
LOCUS       DAOSDX010000369         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13491_length_2659_cov_3.489631, whole genome shotgun sequence.
ACCESSION   DAOSDX010000369 DAOSDX010000000
VERSION     DAOSDX010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13491_length_2659_cov_3.489631"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..935)
                     /locus_tag="PLN02_08680"
     CDS             complement(42..935)
                     /locus_tag="PLN02_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOI52438.1"
                     /translation="MRLGIDLGGSKIEIVALASDGSERLRRRIATPQGDYRATLQAIA
                     GLVASAENETGELGSVGIGIPGAESLLDGRIKNANSTCLNGQPLRADLRTLLQREVRL
                     ANDANCFALSEAVDGAGRGARCVFGVILGTGVGGGIVIDGKVLVGANAIAGEWGHNPL
                     PPAAADPLPAQPCYCGRQGCVETWLSGPGLAADHRRINGDELSALEIAERAAAGDTAC
                     AATLARHLARLGRALAGVINLLDPDVVVLGGGLSNIRQLPQHLPEACLPHVFSDACRT
                     RFLPAVHGDSSGVRGAAWLWD"
     gene            complement(935..1585)
                     /locus_tag="PLN02_08685"
     CDS             complement(935..1585)
                     /locus_tag="PLN02_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI52439.1"
                     /translation="MHKNIQPSPWAEENDPIQQLALEECVKRQRFDNAVSVLVLPAGG
                     AKLASRFAALGARVTLADRAERKQEIEGRVLAAGYPDHIRFAATELPSPPPQDPNAPY
                     DIIVFRHGLCKLPYARGKQLIQQLMQQLKIGGKLYLSIIGLHSELGEGYAGHEQSIYE
                     RWGKLSPAMARKYDVRDEVCLYSERDLFLLLLESGASVLRTLTTTWGNVKGVAVRV"
     gene            1892..>2659
                     /locus_tag="PLN02_08690"
     CDS             1892..>2659
                     /locus_tag="PLN02_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOI52440.1"
                     /translation="MKRIALAAALATVFSAAAVTPAFAQEASTLKKIKDTGSITLGHR
                     ESSIPFSYYDDKQQVIGYSHELMLKVVDAVKADLKLAKLDTKLMPVTSANRITLLQNG
                     TVDIECGSTTNNLERQKQVGFSTTIFVIGTKLLTKKTSGINDFADLAGKNVVTTAGTT
                     SERLIRKMNEEKKLGMNVISAKDHGESFLTLETGRAVAFMMDDALLYGEMAKAKRPGD
                     WVVVGTAQSKEAYGCMLRKDDAEFKKVVDAALTKAMTS"
BASE COUNT          460 a          841 c          909 g          449 t
ORIGIN      
        1 gccgccgacg aagagggaga tctgtgccat ggattgcgct gtcagtccca cagccaggcc
       61 gcgccgcgca cgccggacga gtcgccatgg acggccggca ggaagcgggt ccggcaagcg
      121 tcggaaaaga cgtgtggcag gcaggcttcg ggcaggtgtt gggggagttg ccggatgttg
      181 gagaggccgc cgccgaggac gacgacgtcc gggtcgagca ggttgatgac gccggccagg
      241 gcgcgaccaa ggcgggcgag gtggcgcgcc agcgtcgccg cgcaggcggt gtcgccagcg
      301 gcggcacgct cggcgatctc cagcgcgctc agttcgtcgc cgttgatccg ccggtgatcg
      361 gccgccaagc cggggccgga cagccaggtt tcgacgcagc cctgacggcc gcaatagcag
      421 ggctgggcgg gcagggggtc ggccgccgct ggcggcagcg ggttgtgccc ccactcgccg
      481 gcgatcgcat tggcaccgac cagcaccttg ccgtcgatga cgatgccgcc gccgacgccg
      541 gtgccgagga tgacgccaaa gacgcaacgg gcgccgcgac cggcgccgtc gaccgcttcc
      601 gagagggcga agcagttggc gtcgttggcc aggcggactt cccgctgcag cagcgtccgc
      661 aggtcggcgc gcaggggctg gccgttgagg caggtcgaat tggcgttctt gatccgcccg
      721 tcgagcagcg actcggcgcc gggaatgccg atgccgacgc tgccgagttc gccggtttcg
      781 ttctcggcgc tggcgaccag gccggcgatc gcctgcaggg ttgcccggta gtcgccctgc
      841 ggcgtcgcga tgcggcgacg caggcgctcg ctgccgtcgc tggcgagggc gacgatttcg
      901 atcttgctgc cgccgaggtc gatccccagc cgcatcagac gcgcacggcg acgcccttga
      961 cgttgcccca ggtggtggtc agcgtgcgca gcacactggc gccggattcg agcagcagca
     1021 ggaagaggtc gcgctcggaa tagaggcaga cttcgtcgcg gacgtcgtac ttgcgcgcca
     1081 tcgccggcga aagcttgccc cagcgttcgt agatcgactg ttcgtggccg gcgtagcctt
     1141 cgcccagttc ggaatgcagg ccgataatgg aaaggtagag cttgccgccg atcttcagtt
     1201 gctgcatcag ttgctggatc aactgcttgc cgcgggcgta gggcagtttg cacaggccgt
     1261 ggcggaaaac gatgatgtcg tagggcgcgt tcgggtcttg cggcggcggc gagggcagct
     1321 cggtggcggc gaagcggata tggtccggat agccggcggc gaggacgcgg ccttcgattt
     1381 cctgcttgcg ctcggcccgg tcggccaggg tgacccgcgc gccgagcgcg gcgaagcggc
     1441 ttgccagttt ggcgccaccg gccggcagga cgagtactga gaccgcgttg tcgaagcgct
     1501 gacgcttgac acactcttcc agcgccaact gctggatcgg gtcgttctcc tccgcccagg
     1561 gactgggttg gatattcttg tgcatgatct ctctccccct ttccgggcat tgtgcatgag
     1621 gcgcccgatc cctgacaact cgcgactctc cagaggcgag ccggggttgg cgacgcttcg
     1681 tgggcggcgt ggcgatccca gtatcggaaa tccgatggcg tactttcccg aaatatcggg
     1741 tttccgttgg cgctgaaccc cggtcctcga tccattttca tagaaatcaa cggtttgccg
     1801 attgtcggcg tagcgacggc ggctggcacg tggcttgcga tgtattctcc gccgaagccc
     1861 gttcgcggct tcctcacttt caggagaaga catgaaacgt atcgccctcg ccgccgccct
     1921 ggccaccgtg ttttccgctg ccgccgtgac gccggcgttt gcccaggaag cctcgacgct
     1981 gaagaagatc aaggacacgg gcagcatcac gctcggtcat cgcgaatcct cgatcccgtt
     2041 ttcttactac gacgacaagc agcaagtcat cggctactca catgaactga tgctcaaggt
     2101 ggtcgacgcg gtgaaggccg atttgaagct ggccaagctc gacaccaagc tgatgccggt
     2161 cacttccgcc aaccgcatca cgctgctcca gaacggcacc gtcgatatcg aatgcggctc
     2221 gaccaccaac aacctggagc gccagaagca ggtcggtttc tcgaccacca tcttcgtcat
     2281 cggcaccaag ctgctgacca agaagacctc gggcattaac gacttcgccg acctcgccgg
     2341 caagaacgtc gtcaccaccg ccggcacgac gtccgagcgc ctgatccgca agatgaacga
     2401 agagaagaag ctgggcatga acgtgatttc ggccaaggat cacggcgaaa gcttcctgac
     2461 cctggaaacc ggccgtgccg tcgccttcat gatggacgac gcgctgctct acggcgaaat
     2521 ggccaaggcc aagcggccgg gtgactgggt cgtggtcggc acggcgcaat cgaaggaagc
     2581 ctacggctgc atgctgcgca aggacgacgc cgagttcaag aaggtggtcg atgccgcgct
     2641 gaccaaggcg atgacttcc
//
LOCUS       DAOSDX010000370         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13560_length_2651_cov_2.717643, whole genome shotgun sequence.
ACCESSION   DAOSDX010000370 DAOSDX010000000
VERSION     DAOSDX010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13560_length_2651_cov_2.717643"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLN02_08695"
     CDS             complement(<1..313)
                     /locus_tag="PLN02_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOI52441.1"
                     /translation="MSEHTLHFKPAEGFRARSKAVVDNPFLRQSFRGAMDFLMSKRAA
                     QFPDPEELESLRSLGEAIRQYNLAKLPDLLVQLEANLTRNGVKVHWAETPDEANAIIL
                     GI"
     gene            complement(310..978)
                     /locus_tag="PLN02_08700"
     CDS             complement(310..978)
                     /locus_tag="PLN02_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HOI52442.1"
                     /translation="MSARDRILGKLRQHAPQTAKPLPDVGSWYAMHRPLEAAAEKARR
                     FRHFIELAHAEVHAVTAANWTETLHAVLKARAIDKLLVAEGTPHGDAALDALPPRGIE
                     CSVYDMAIEAWKSEMFNATPAGLTAARAAIAETGTLILWPNALEPRLMSLVPPVHIVL
                     LDASKIYNTFFEAMSVEGWKNGLPTNSLLISGPSKTADIQQTLAYGAHGPKELIVLLL
                     EGEA"
     gene            complement(975..1763)
                     /locus_tag="PLN02_08705"
     CDS             complement(975..1763)
                     /locus_tag="PLN02_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOI52443.1"
                     /translation="MTNPTTQTSRPSDVYFFATCVVDQFFPGAGLDAITLLERQGIRV
                     HFPEDQTCCGQPAYSSGFEEEARRVAAHQLTLFPNDWPIVVPSGSCAGMMKHHYPKLF
                     AADPTRKAVAERLSARVYELTQFLVEVLDFQPQDCGGDCKVVLHTSCSARREMGVHLT
                     GRKLLAGLEKVSVVQQDHESECCGFGGTFSVKQPEISGAMVEDKVKALKATGAERVVS
                     ADCGCLMNILGHAAWKDQQAGRSEPSLPGEHIASFLLRRTGGGK"
     gene            1950..>2651
                     /locus_tag="PLN02_08710"
     CDS             1950..>2651
                     /locus_tag="PLN02_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOI52444.1"
                     /translation="MPNKVQVQRISDAVASTLERRILEGSLKPGDRLPAERELALELG
                     VSRPSLREAIQKLASKGMLQSRQGGGTYVTAALEKSFFDPWQEMMGSYPNLREDMLEF
                     RRMIEGQAAEWAAERATEADLLRLDQAFGALQTAFQSDDTDKRSAVDIAFHQAIGDAS
                     HNVLIGHLSAALLRLMHDNIRLNLGELKSVPAASRLLLTQHEAIYRAIRDSKPQAARA
                     AAETHIDFVRETLAHS"
BASE COUNT          439 a          826 c          900 g          486 t
ORIGIN      
        1 tgatgccgag gatgatcgcg ttcgcctcgt caggcgtctc cgcccaatgc accttgacgc
       61 cgttgcgggt caggttggct tcgagttgca cgagcaggtc gggcagcttg gccaggttgt
      121 actgacggat cgcttcgccc aggctgcgca ggctttccag ttcctcgggg tcggggaact
      181 gggcggcgcg cttgctcatc aggaaatcca tcgcgccgcg gaagctctgg cgcaggaagg
      241 gattgtcgac gaccgccttg gaacgggcgc ggaaaccctc ggccggcttg aagtgcaagg
      301 tatgttcgct catgcctcgc cctccagcag caggacgatc agttccttcg ggccgtgcgc
      361 gccgtaggcc agcgtctgct ggatgtcggc ggtcttcgac gggccggaga tgagcagcga
      421 attggtcggc aggccgttct tccagccttc gaccgacatc gcctcgaaga aggtgttata
      481 gattttcgac gcgtcgagca gcacgatgtg caccggcggt accagcgaca tcagccgcgg
      541 ctccagcgcg ttcggccaga ggatcagtgt gccggtctcg gcgatcgcgg cgcgggcggc
      601 ggtcaggccg gccggcgtgg cgttgaacat ttccgacttc caggcctcga tcgccatgtc
      661 gtagacgctg cattcgatgc cgcgcggcgg cagggcgtcg agcgccgcat cgccgtgcgg
      721 cgtgccttcg gcgaccagca gcttgtcgat ggcgcgcgcc ttgagtacgg cgtgcagcgt
      781 ctccgtccag ttggcggcgg tcaccgcatg cacttcggcg tgcgccagtt cgatgaaatg
      841 acggaagcgc cgggcttttt ccgcggccgc ttcgagcggg cgatgcatgg cgtaccagct
      901 accgacgtcg ggcagcggtt tggccgtttg cggcgcgtgc tggcgcagct tgccgaggat
      961 gcggtcgcgg gcgctcattt tccacctccg gtacggcgca gcaggaagct ggcgatgtgt
     1021 tcgccgggca gactgggctc gctgcggccg gcctgctggt ccttccaggc ggcgtggccg
     1081 aggatgttca tcaggcagcc gcagtcggcg ctgacgacgc gttcggcacc ggtggccttg
     1141 agcgccttga ctttgtcctc gaccatggcg ccggagattt ccggctgctt gaccgagaag
     1201 gtaccgccga agccgcagca ttcgctttcg tgatcctgct ggacgacgct gaccttctcc
     1261 agtccggcca gcagcttgcg accagtgaga tggacgccca tctcgcggcg cgccgagcac
     1321 gaggtgtgca ggacgacctt gcaatcaccg ccgcaatcct gcggctggaa atcgaggacc
     1381 tcgaccagga actgcgtcag ctcgtagacc cgcgccgaca ggcgctcggc aaccgccttg
     1441 cgggtcgggt cggcggcgaa cagcttgggg tagtggtgtt tcatcatgcc ggcgcaggag
     1501 ccggacggaa cgacgatcgg ccagtcgttg ggaaacagcg tcagttggtg ggcggcaacg
     1561 cgccgcgctt cttcttcgaa gccgctgctg taggccggct ggccgcagca ggtctggtct
     1621 tccggaaagt gcacgcggat gccctggcgt tcgagcaggg tgatggcgtc gaggccggcg
     1681 ccgggaaaga actggtcgac gacgcaggtc gcaaagaaat agacgtcgct tggccggctg
     1741 gtctgggttg ttgggtttgt catcctcgct ccgcgttttt atttgatgga taaatggtaa
     1801 gaccacttgt gtcgcggaat ggaaatttaa ggcaaaaccg taacttggtc aatgcatcaa
     1861 ggagatggtc aagggaaatc cctaagcttg cgctttgttt ttcggctggt tagaattgcc
     1921 cggtcttacc aatcatggtg gtcttaccaa tgccgaacaa ggtgcaggtt cagcggattt
     1981 ccgatgccgt cgcttcgacg ctggaacgcc gcattctcga aggctcgctg aagccgggtg
     2041 accgcctgcc ggcggaacgc gaactggcgc tcgaactcgg cgtgtcgcgg ccgagcctgc
     2101 gcgaggcgat ccagaagctg gcgtccaagg gcatgctgca gagccgccag ggcggcggca
     2161 cctacgtcac ggcggcgctg gagaaaagtt tcttcgatcc ctggcaggaa atgatgggtt
     2221 cctacccgaa cctgcgcgag gacatgctcg aattccgccg catgatcgag ggccaggccg
     2281 ccgaatgggc cgccgaacgc gccaccgagg ccgacctgct gcgcctcgac caggccttcg
     2341 gcgccttgca gacggccttc cagagcgacg acaccgacaa gcgctcggcg gtcgacatcg
     2401 ccttccacca ggcgatcggc gacgcctcgc acaacgtgct gatcggccat ctgtcggcgg
     2461 cgctgctgcg cctgatgcac gacaacatcc gcctcaacct cggcgaattg aagagcgtgc
     2521 cggccgccag ccggctgctg cttacccagc acgaggcgat ctaccgcgcc atccgcgaca
     2581 gcaagccgca ggcggcgcgc gccgccgccg agacacacat cgatttcgtg cgcgagacgc
     2641 tggcccactc g
//
LOCUS       DAOSDX010000371         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13566_length_2650_cov_3.240848, whole genome shotgun sequence.
ACCESSION   DAOSDX010000371 DAOSDX010000000
VERSION     DAOSDX010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13566_length_2650_cov_3.240848"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /gene="dapE"
                     /locus_tag="PLN02_08715"
     CDS             complement(<1..782)
                     /gene="dapE"
                     /locus_tag="PLN02_08715"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOI52445.1"
                     /translation="MTDPTLELARRIMACPSVTPDDAGCMDILGARLAAIGFSLEWLD
                     RNGTRNLWARRGTAAPLFVFAGHTDVVPTGPLDKWTSPPFAPEIRDGKLYGRGAADMK
                     TGVAAAVTAVEAFVAAHPGHPGSLAFLLTSDEEGDANDGTVAVTEALQARGEHLDYCI
                     IGEPTSVDTLGDMIKNGRRGSLSGVLNVKGIQCHIAYPEKGRNPIHQAAPAIAELAAT
                     EWDQGNEYFPPTTWQISNIHGGTGATNVIPGQLEIKFNFRFST"
     gene            complement(782..1966)
                     /locus_tag="PLN02_08720"
     CDS             complement(782..1966)
                     /locus_tag="PLN02_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOI52446.1"
                     /translation="MILDKLFQLMSEKQASDIFISAGTPIHIKIQGTTMPVNQQIMLP
                     DMIEKIAYELMNEDQAKTFEATNEMNLSFGVPQVGNFRINLFRQRGSIGIVVRFILGN
                     IPPLDSLGMPPLLGELIMEKRGLILIVGATGSGKSTTIASMLDHRNSTRAGHILTVED
                     PIEFLFKHKKSIVNQREIGMDTHDWNSALKNAMRQAPDCILIGEIRDRDTMQAAISYA
                     QTGHLCLATLHANNSYHALNRIISFFPLENRPALFLDLSVSLRAIVSQRLVRNPEGKR
                     LPTCEIMLNTRHISELIERGEVQGIKDAMEQTLAPGSQTFEQDLFRLYHEGSITLDEA
                     LANADSPTNLSWLINNSEFNSNSGKDEKKPDISLEFDSTDEGGASFKEFQLSLAEDDE
                     TH"
     gene            complement(1993..>2650)
                     /gene="dapD"
                     /locus_tag="PLN02_08725"
     CDS             complement(1993..>2650)
                     /gene="dapD"
                     /locus_tag="PLN02_08725"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOI52447.1"
                     /translation="VNQWAKKAVLLSFRTRDNRVQEAGDVRFYDKVDTKFQGWSEEQF
                     KAGGFRVVPGAFARKGSHIAKNVVLMPSFVNIGAFVDEGTMVDTWATVGSCAQIGKNV
                     HLSGGVGIGGVLEPIQAGPVIIEDNCFIGARSEVVEGVVVGENSVISMGVYIGQSTPI
                     YDRETGEISYGRIPPGSVVISGSLPKDNGRYSLYAAIIVKKVDAQTRSKTSINELLRA
                     "
BASE COUNT          429 a          744 c          941 g          536 t
ORIGIN      
        1 gtggagaagc ggaagttgaa cttgatttcg agctggccgg ggatgacgtt ggtcgcgccg
       61 gtgccgccgt ggatgttgga aatctgccag gtggtcggcg ggaagtattc gttgccctgg
      121 tcccactcgg tcgccgccag ttcggcaatc gccggcgccg cctggtggat cgggttgcgc
      181 cccttttccg ggtaggcgat gtggcactgg atgcccttga cgttgagcac gccggagagc
      241 gagccacggc ggccgttctt gatcatgtcg ccgagcgtat cgaccgaggt cggctcgccg
      301 atgatgcaat agtcgagatg ttcgccacgc gcctgcaggg cttcggtgac ggcgacggtg
      361 ccgtcattgg cgtcaccttc ctcgtcggag gtgagcagga aggcgagcga gccggggtgg
      421 cccggatgcg cggcgacgaa ggcctcgacc gcggtgacgg cggcggcgac gccggtcttc
      481 atgtcggcgg cgccgcgccc gtagagcttg ccgtcacgga tttccggggc gaacggcggg
      541 ctggtccatt tgtcgagcgg gccggtcggt acgacgtcgg tatggccggc gaagacgaac
      601 agcggtgcgg cggtgccacg gcgcgcccag aggttgcggg tgccgttgcg gtccagccat
      661 tcgagggaaa agccgatggc ggccagacga gcgccgagta tgtccatgca gccggcgtcg
      721 tcgggcgtga cggacgggca cgccatgatc cggcgtgcca gttcgagggt gggatcggtc
      781 atcagtgggt ttcgtcgtct tcggcgaggc tgagctggaa ttccttgaac gaggcgccgc
      841 cttcgtcggt gctgtcgaat tcgagcgaga tgtcgggctt cttctcatcc ttgcccgagt
      901 tggaattgaa ctcggagttg ttgatcagcc acgacaggtt ggtcggcgaa tcggcgttgg
      961 ccagcgcttc gtcgagagtg atcgagcctt cgtggtagag gcggaagaga tcctgttcga
     1021 aggtctgcga tccgggcgcc agtgtctgct ccatcgcgtc cttgatgccc tggacttcgc
     1081 cgcgctcgat cagttcgctg atgtggcgcg tgttgagcat gatttcgcag gttggcaggc
     1141 gcttgccttc cggattgcgt accagacgct gcgagacgat cgcgcgcagg gagaccgaga
     1201 ggtcgaggaa gagcgccggc cggttttcca gcgggaagaa gctgatgatg cggttgagcg
     1261 catggtagga gttgttggcg tgcagtgtcg ccagacagag gtggccggtc tgggcgtagg
     1321 aaatcgccgc ctgcatggta tcgcggtcac ggatctcgcc gatcaggatg cagtccggcg
     1381 cctggcgcat cgcgttcttc agcgcggaat tccagtcgtg cgtgtccatg ccgatttcgc
     1441 gctggttgac gatcgatttc ttgtgcttga acaggaattc gatcgggtcc tcgacggtca
     1501 ggatgtggcc ggcgcgcgtc gagttgcggt ggtcgagcat cgaggcgatg gtcgtcgact
     1561 tgccggaacc ggtggcgccg acaatcagga tcaggccgcg cttttccatg atcagctcgc
     1621 cgagcagcgg cggcatgccg aggctgtcga gcggcgggat gttgccgagg atgaagcgga
     1681 cgacgatgcc gatcgaaccg cgctggcgga acaggttgat gcggaagttg ccgacctgcg
     1741 gcacaccgaa cgagaggttc atctcgttgg tggcctcgaa ggtcttggcc tgatcctcgt
     1801 tcatcagttc ataggcgatc ttctcgatca tgtcgggcag catgatctgc tggttgaccg
     1861 gcatggtcgt accctggatc ttgatgtgga tcggcgtgcc ggcggaaatg aagatatccg
     1921 atgcctgctt ttccgacatc agctggaaca gtttgtcgag gatcatggca agacctcttt
     1981 cgcgaaaggt agttaggcgc gcaacagttc gttgatgctg gtcttcgagc gcgtctgggc
     2041 gtcgaccttc ttgacgatga tcgccgcgta caggctgtag cgaccgttgt ccttgggcag
     2101 cgagccggag atcacgaccg agcccggcgg gatgcggccg tagctgattt caccggtctc
     2161 gcggtcgtag atcggcgtgc tctggccgat gtacacgccc atcgaaatca ccgagttctc
     2221 gccgacgacg acgccttcga cgacttccga gcgggcgccg atgaagcagt tgtcttcgat
     2281 gatcaccggg ccggcctgga tcggttcgag cacgccgccg atgccgacgc cgcccgagag
     2341 gtgcacgttc ttgccgatct gcgcgcagga gccgacggtg gcccaggtgt cgaccatcgt
     2401 gccttcatcg acgaaggcgc cgatgttgac gaaggacggc atcaacacga cgttcttcgc
     2461 aatgtgcgaa cccttgcgcg cgaaggcacc gggcacgacg cggaagccgc cggccttgaa
     2521 ctgctcctcg ctccagccct ggaacttggt atcgaccttg tcgtagaagc gcacgtcgcc
     2581 ggcttcctgg acgcggttgt cgcgggtgcg gaaggagagc agaacggcct tcttggccca
     2641 ctggttgacc
//
LOCUS       DAOSDX010000372         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13589_length_2647_cov_3.020833, whole genome shotgun sequence.
ACCESSION   DAOSDX010000372 DAOSDX010000000
VERSION     DAOSDX010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13589_length_2647_cov_3.020833"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /gene="glcF"
                     /locus_tag="PLN02_08730"
     CDS             <1..784
                     /gene="glcF"
                     /locus_tag="PLN02_08730"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HOI52448.1"
                     /translation="KLPPEPAAARPWPATGQHPRRMLALAGCVQPSLAPNINAATARV
                     LDRLGIELFEEKKAGCCGAVRHHLNDQAAARADMRRNIDAWWPHVEAGVEAIVVTASG
                     CGVQVRDYGHLLADDADYAEKAARVSALCRDPSEVVAAEGGRLRALLAPAERGKLAFQ
                     SPCTLQHGLKIRGVVENLLAAAGYLLTPVADGHLCCGSAGTYSLLQPALSQQLRANKL
                     AALTAGGPALIATANIGCLTHLQAGATTPVRHWIELIDEALG"
     gene            781..1674
                     /locus_tag="PLN02_08735"
     CDS             781..1674
                     /locus_tag="PLN02_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOI52449.1"
                     /translation="MSSKGNSVPEITRSLGAEDAARVRRVLEGGIKIPPQPRVLDEMR
                     RMMNRQEYDVRRLARTIQQDPGLSAMLFKVVGNAAYRQHQPFETIEQILHAVGLKQTF
                     NLVQAIALIDAGTPTADKRIYEAFWSRSQAVGQLAMLVAEERVAVCNIFADQAYLAGI
                     FHACGVPLLLQRFSSYGMELRLATPGHWVDWREEDRKFNTDHAVVGYLVARHWCLPGF
                     ICDAIRYQHSLAELGDHEARSMVAILALAIDIHARDQRIPNPEWDEVRNVLLPELGLD
                     EESLPEFIDVVLERFNHATHV"
     gene            1724..2485
                     /locus_tag="PLN02_08740"
     CDS             1724..2485
                     /locus_tag="PLN02_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOI52450.1"
                     /translation="MAIAIDACAAQHQGDRREQQDRVAILPHPRSRGVALAVVADGMG
                     GHTGGALAAEQVIHTARNNLEHFAPSEESARWLLENSMREAHTMIKASRFMNEKDPHS
                     TAVMLLLQPGKLSWGYCGDSRFYAYRGRQLLTRTVDHSYVEHLVAQGKITAAQALTHP
                     NRNILVTSLGGQDEPLFTFGETADLQPGDSFMLCSDGVWGYFDEAELGAALADNTARG
                     ACETIIEQARRRAAGGGDNLSLAVIRLLKPAAPAR"
BASE COUNT          447 a          857 c          902 g          441 t
ORIGIN      
        1 caagctgccg ccggagccgg cggcggcccg cccgtggccg gccacgggcc agcatccgcg
       61 gcgcatgctg gcgctcgccg gctgcgtgca gccttcgctg gcgccgaaca tcaacgcggc
      121 gacggcgcgc gtgctcgacc gcctcggcat cgaactattt gaagaaaaaa aggccggctg
      181 ctgcggtgcc gtgcgccatc acctcaacga tcaggcggcg gcccgggccg atatgcggcg
      241 caacatcgac gcctggtggc cgcatgtcga agccggggtc gaggccatcg tcgtcaccgc
      301 ctccggttgc ggcgtccagg tgcgcgatta tggccacctg ctggccgacg atgcggacta
      361 cgccgaaaaa gcggcacggg tcagcgcgtt gtgccgcgac ccgtcggaag tggtcgccgc
      421 cgaaggcggt cgcttgcgcg ccttgctggc accggccgaa cgtggcaagc tcgcctttca
      481 gtcgccatgt acgctgcaac acggattgaa gattcgcggt gttgtggaaa acctcctggc
      541 agcggccggg tatctgctga cgccggtggc cgatggccac ctgtgctgcg gttcggcggg
      601 aacgtattcg ctgctgcagc cggcgctgtc gcaacagctg cgcgccaaca agctggcggc
      661 gctgaccgcc ggcggtccgg cactgatcgc caccgccaat atcggttgcc tgacacactt
      721 gcaggccggc gcgacgacgc cggtccgcca ctggatagaa ttgattgacg aggctttggg
      781 atgagcagca aggggaattc ggtgccggaa atcacgcgtt cgctgggggc ggaagacgcc
      841 gcccgggtgc ggcgcgtcct tgagggcggc atcaagattc cgccccagcc gcgcgttctc
      901 gacgagatgc ggcggatgat gaaccgccag gaatacgatg ttcgccgcct ggccaggacc
      961 atccagcagg acccagggct gagcgcgatg ctgttcaagg tggttggcaa cgcggcgtat
     1021 cgccagcacc agccgttcga gaccattgag cagatcctgc acgcggtcgg cctcaagcag
     1081 accttcaacc tggtgcaggc gatcgccctg atcgacgctg gcacgccgac ggccgacaag
     1141 cgcatttacg aggccttctg gtcgcgttcg caggcggtcg gccagctggc gatgctggtt
     1201 gccgaggagc gcgtcgccgt ctgcaacatc ttcgccgatc aggcctacct ggccggcatc
     1261 tttcacgcct gcggcgtgcc tctgctgctg cagcgctttt cttcctacgg catggagttg
     1321 cgtctggcga cgccggggca ctgggtggat tggcgggaag aggatcgcaa gttcaatacc
     1381 gaccacgccg tcgtcggcta tctggtcgcc cgtcactggt gcctgcccgg attcatctgc
     1441 gacgccatcc gttaccagca ttcgctggcc gagctgggcg atcacgaagc acgcagcatg
     1501 gtggcgatcc ttgccctggc catcgacatc cacgcccgcg accagcggat tccgaatccc
     1561 gagtgggacg aggtgcgcaa cgtactgctg cctgaactcg gactcgacga agaatccttg
     1621 ccggaattca tcgatgtcgt gctcgaacgc ttcaatcacg ccacccacgt ttgattccgg
     1681 gcgcggtgtt tcgtgctaat ctgcccggca aaagggagga atgatggcga tagccattga
     1741 tgcctgcgcg gcacaacatc agggcgaccg gcgcgaacaa caggatcggg tggcgatcct
     1801 gccgcatccg cgcagccggg gcgtcgcgct ggccgtcgtt gccgacggca tgggcggcca
     1861 caccggtggc gcgctggcgg ccgagcaggt gatccacacc gcgcgcaaca atctcgagca
     1921 tttcgcgccg agcgaggaaa gcgcccgctg gctgctggaa aacagcatgc gcgaggcgca
     1981 cacgatgatc aaggcgtcgc gtttcatgaa cgagaaggac ccgcacagca cggcggtgat
     2041 gctgctgctg cagccgggaa agctcagttg gggttattgc ggcgacagcc gcttctacgc
     2101 ctaccgcggt cgccagttgc tgacgcgcac ggtcgatcat tcctatgtcg aacatctggt
     2161 ggcgcagggc aagatcactg cggcgcaggc gctgacccat cccaaccgca acatcctggt
     2221 gacctcgctt ggcggccagg atgaaccgct gttcaccttc ggcgaaaccg ccgacctgca
     2281 gcccggcgac agcttcatgc tgtgctcgga cggggtctgg ggttatttcg acgaagccga
     2341 actgggcgca gcgctggccg acaacacggc gcgcggagct tgcgagacga tcatcgagca
     2401 ggcgcggcgg cgggcggcgg gcggcggcga caacctgtcg ctggcggtga tccgcctgct
     2461 caagccggca gcgccggccc gctgaagaga cgtctcagga cttgcggccg aggccgccga
     2521 gcagcgcggc aaccacccgt ttcggcttgt gcgggtcggc cttggccggt gcttgcggcg
     2581 aatcgggaat ctcgccctgc ggcttggtcg attcgtaggg cttgctgaag tcgaagccgt
     2641 cggcggc
//
LOCUS       DAOSDX010000373         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13597_length_2646_cov_4.394056, whole genome shotgun sequence.
ACCESSION   DAOSDX010000373 DAOSDX010000000
VERSION     DAOSDX010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13597_length_2646_cov_4.394056"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2639)
                     /gene="vgrG"
                     /locus_tag="PLN02_08745"
     CDS             complement(<1..2639)
                     /gene="vgrG"
                     /locus_tag="PLN02_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HOI52451.1"
                     /translation="MSIDLVRNLFSSDTRLYALSWRDEGMPQLEVECWWGRESLAGGF
                     EYVIDLVSPDARLGQSRFLGKALALDVRLADGSTFKRHGYVRWAATLDADGGFGRYRL
                     IVIPWLWLTSRGRHHRVFQERSVVDIVDTVFADYADIAAWQWADEVAGFLADTRPRSY
                     CVQYNESDYQFVSRLLAEEGLGWRVEADDAAPGGHRLTIFADSRLLPQDPVSASGLRY
                     HRSDSQEAQDAILAFGEHRRLLAAKTTLLSHDYKAKRAIASSVPTAQPVGGPHAPLLE
                     NYDDAGPYAFASLAEAERYGRLTMEAHEARYLTWLGRGTVRSLSAGTWIAIGDLPRAG
                     DHRYLVCEVNHAGINNLTAAAMARLAERLRLPQNLGQDDPLSGGDAVEPVAAPARVPA
                     ELLELARERGYGNSFTGLHADLPWRPALSDDTGLRLNPRPTAPGPMTATVVGPNGEQS
                     PNGADEIWCDRYGRIKIRFPWQQAERPDDRDSCWMRVLQRQAGPGMGWQWLPRIGQEV
                     LIDFLNGDMDRPYVLGALYNGKGAGGVPATPGGRPGESDASVFDPATDHRPAGQGNLA
                     GGNSPAWHGGASHSHRHPAALGGFKSKEFGGQGFNQLVLDDSDRQQRIQLKSTQHSSE
                     LNLGHLIHQADNYRGSFRGTGVELRTDAWGALRGGRGVLMSTWPLATPGTPAGDFAPG
                     MALLRQADTLARTLSQAAKTHRTVELASAIGSNAAEQSAIDQQAAPIKALHTVASGMV
                     DAKDENQASADAQAKTITADTDKRPHPTDPAILQAARANFAHVAGQNLQYANDETTTF
                     ESGEDSNLAIAGKARIHTGQAIGLVAGAIRPGEGNTGIKLIAAKDDIDLQAQSDEMKF
                     QAKQDLKIVSANAHVDFAAAKKI"
BASE COUNT          298 a          800 c         1064 g          484 t
ORIGIN      
        1 tgaatcttct tcgccgccgc gaagtcgacg tgggcattcg ccgagacgat cttcaggtcc
       61 tgcttggcct ggaacttcat ttcatcgctt tgcgcctgca ggtcgatatc gtctttggcg
      121 gcgatcagtt tgatgccggt gttgccttcg ccggggcgga tggcgccggc gacgaggccg
      181 atggcctggc cggtgtggat tctggctttg ccggcgatgg cgaggttgct gtcttcgccg
      241 ctctcgaagg tggtggtttc gtcgttggcg tattgcaggt tctgaccggc aacgtgggcg
      301 aagttggcgc gggcggcttg caggatcgcc gggtcggtcg ggtgcgggcg tttgtcggtg
      361 tcagccgtga tggtcttggc ctgggcgtcg gcgctggcct ggttttcgtc tttggcgtcg
      421 accatgccgc tggcgacggt gtgcagggcc ttgatcggtg cggcttgttg gtcgatggcg
      481 ctttgttcgg cggcgttgct gccgatggcg ctggcgagtt cgacggtgcg gtgggtcttc
      541 gccgcctggc tgagggtgcg ggccagggtg tcggcttgtc tgagcagggc catgccgggg
      601 gcgaagtcgc cggccggggt gccaggggtg gcgagcggcc aggtgctcat cagtacgccg
      661 cgcccgccgc gcagcgcgcc ccaggcgtcg gtccggagtt cgacgccggt gccgcggaag
      721 ctgccacggt agttgtcggc ctggtggatg aggtggccga ggttgagttc gctgctgtgc
      781 tgggtgctct tgagctggat gcgttgctgg cggtcgctgt cgtccagcac cagttgattg
      841 aacccctggc cgccgaattc cttgctcttg aagccgccga gtgcggccgg gtggcggtgg
      901 ctgtggctgg cgccgccgtg ccaggcgggg ctgttgccgc cggcgaggtt gccctggccg
      961 gccgggcggt ggtcggtggc ggggtcgaag acgctggcgt cgctttcgcc cgggcggccg
     1021 cccggggtgg cggggacgcc gccggcgccc ttgccgttgt agagggcgcc gagcacgtag
     1081 gggcggtcca tgtcgccgtt caggaagtcg atcagcactt cctggccgat gcgtggcagc
     1141 cattgccagc ccatgccggg gccggcctgc cgttgcagga cgcgcatcca gcagctgtcg
     1201 cggtcgtccg gccgttcggc ttgttgccag gggaagcgga tcttgatccg gccgtagcgg
     1261 tcgcaccaga tttcgtcggc gccgttcggg ctctgctcgc cgttggggcc gacgacggtg
     1321 gcggtcatcg ggccgggggc ggtggggcgc gggttgaggc gcagtccggt gtcgtcgctg
     1381 agcgccggcc gccatgggag gtcggcgtgc agaccggtga agctgttgcc gtagccgcgt
     1441 tcgcgggcga gttcgaggag ttcggcgggc accctggccg gggcggcgac cggttcgacg
     1501 gcgtcgccgc cggagagcgg gtcgtcctgg ccgaggttct gcgggaggcg caggcgttcg
     1561 gcgaggcggg ccatggcggc ggcggtcagg ttgttgatgc cggcgtggtt cacttcgcag
     1621 accaggtagc ggtggtcgcc ggcgcgcggc aggtcgccga tggcgatcca ggtgccggcg
     1681 ctgaggctgc gcacggtgcc gcggccgagc caggtcaggt agcgggcttc gtgcgcttcc
     1741 atcgtcagcc gcccgtagcg ttcggcttcg gccaggctgg cgaaggcgta ggggccggcg
     1801 tcgtcgtagt tctcgagaag cggcgcatgc ggccccccga ccggctgcgc ggtcggcacg
     1861 ctgctggcga tggcgcgctt ggccttgtag tcgtgcgaga gcagcgtggt tttggcggcg
     1921 agcaggcggc ggtgttcgcc gaaggcgagg atggcgtcct gggcttcctg cgagtcgctg
     1981 cggtggtagc gcaggccgct ggcggacacc gggtcctgcg gcagcaggcg gctgtcggcg
     2041 aagatcgtca ggcggtggcc gccgggcgcc gcgtcgtcgg cttcgacgcg ccagcccagg
     2101 ccttcctcgg cgagcaggcg ggagacgaac tggtagtccg attcgttgta ctgcacgcag
     2161 tagctgcgcg gccgggtgtc ggcgaggaag ccggcgacct cgtcggccca ttgccaggcg
     2221 gcgatgtcgg cgtagtcggc gaacacggtg tcgacgatgt cgaccacgct gcgttcctgg
     2281 aagacccggt gatggcggcc gcgcgaagtc agccagagcc aggggatgac gatcagccgg
     2341 tagcggccga aaccgccgtc ggcgtcgagc gtcgccgccc agcgcacgta gccgtggcgc
     2401 ttgaaggtgc tgccgtcggc cagccggacg tcgagcgcca gcgccttgcc gaggaaacgc
     2461 gactggccga gccgggcatc cggcgacacc aggtcgatga cgtactcgaa gccgcccgcc
     2521 aggctctccc gcccccacca gcattcgact tccagttgcg gcatcccctc gtcccgccag
     2581 gacagcgcat acaggcgcgt gtcgctcgag aacaggttgc gcacgaggtc gatggacatg
     2641 gcggtg
//
LOCUS       DAOSDX010000374         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13610_length_2644_cov_2.237157, whole genome shotgun sequence.
ACCESSION   DAOSDX010000374 DAOSDX010000000
VERSION     DAOSDX010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_2644_cov_2.237157"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..879)
                     /locus_tag="PLN02_08750"
     CDS             complement(196..879)
                     /locus_tag="PLN02_08750"
                     /inference="COORDINATES: protein motif:HMM:NF022885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2894 domain-containing protein"
                     /protein_id="HOI52452.1"
                     /translation="MADRGDLQRRLDALRAGGAAERDPVRFAYLDALARRAATQPASI
                     RQVLAGKFGAGADELLSRPAPTPTASAPSDRASPLATLLAYIGQQAHAQPESNPLANA
                     PARLPGDQRPQAKDSVGASRPSAPELKSVAYFRDEWSKLSTEQQLTQTLAQAPENAGP
                     MNSQHLVLRSLQLMRDIAPDYLHGFMSYIDALIWLEHAAPVKPAPARAAASEGVPAKR
                     ATRSSPKRR"
     gene            complement(872..1516)
                     /locus_tag="PLN02_08755"
     CDS             complement(872..1516)
                     /locus_tag="PLN02_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI52453.1"
                     /translation="MEDIDASVDHSTPVWAVFGDLMAGLLGAFVLILVGVLGVQLELV
                     SSLEAEIQKRQQEEQRRLALEKALAGPLASGRVTLDNGKIGISGNVLFALNSDQLQPE
                     GKQLLKSLSQPIAAYLGASEQMLMVSGFTDDMPVRGSNSRFADNWELSAQRALTVTRT
                     LIEDGVPSSSIFAAAFGSQQPVADNADAEGRARNRRVEMAPVPKSRTNAKDANG"
     gene            complement(1519..>2644)
                     /locus_tag="PLN02_08760"
     CDS             complement(1519..>2644)
                     /locus_tag="PLN02_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF802 domain-containing protein"
                     /protein_id="HOI52454.1"
                     /translation="LSVFADTFGQRSQALLDSVAGTHAALKGELATSHAAFAQTTQAQ
                     QAAISEQVAGQLDGIARRFDGAVQTVAETWTGALAKHEQASDRLTQALEQTQISLADT
                     FAQRTAALLTDMRATQADWQRGWASGEQARQSQFADALAAMAGKLEAQWQQHGDATLA
                     QQEQITRTLGDTARELVAAHQRQAETTIAEVTRLMHTAAEAPKAAAEVIGQLRHELSA
                     SMARDNSLLEERSRIMETLGALLDAINHASTEQRSAIDALVASSAELLERAGSQFAAR
                     IEREAGKLVEVGSGIAGGALEVASLGEAFGAAVKLFAESNDKMMAALQRIENGLAKSL
                     TRSDEQLAYYVAQAREIIDLSILSQKKMVDDLQRVAASEA"
BASE COUNT          342 a          797 c          989 g          516 t
ORIGIN      
        1 tgacttgaat ctgggttgtc gcgtgctgcc acgtggcgat gccgcgtgaa attcctaatt
       61 ctaagtgcaa cacgtgattc gaggcgctgg tgatattggg tgagccagac gttcgttcag
      121 ccaacgatcg aactggccca ttctccacct atcaaaattt tgcggcctga gcgtcaggcc
      181 aggggacggg ttcgatcagc gacgcttcgg gctgctcctg gtcgcccgct tcgccggtac
      241 tccctcgctg gcggcggcac gcgccggcgc tggcttgacc ggggccgcgt gttccagcca
      301 gatcagcgcg tcgatgtagg acatgaaacc gtgcagataa tccggcgcga tgtcgcgcat
      361 gagctgcagg ctgcgcagca cgaggtgctg ggaattcatc gggccggcgt tttccggggc
      421 ctgggccagg gtctgtgtca gttgctgctc ggtgctgagt ttggaccact cgtcgcggaa
      481 gtaggcgacg gatttgagtt ccggtgctga cgggcggcta gcgccaaccg aatccttggc
      541 ctggggccgc tggtcgcctg gcaggcgtgc aggcgcgttg gccagggggt tggactctgg
      601 ctgagcgtgg gcctgctggc cgatgtaggc gagcagcgtg gcgagcgggc tggcgcggtc
      661 gctcggcgcg ctcgccgttg gcgtcggtgc cgggcggctg agcagttcgt cggcgccagc
      721 gccgaatttg ccggcgagca cctggcggat gctggccggt tgcgtcgcgg cgcggcgggc
      781 cagggcgtcg aggtaggcga agcggacggg atcgcgctcg gcggcgccgc cggcgcgcag
      841 ggcatcgagt cggcgctgga ggtctccccg gtcagccatt ggcgtccttg gcattggttc
      901 gcgacttggg caccggcgcc atttcgacgc ggcggttgcg ggcgcggcct tcggcgtcgg
      961 cgttgtcggc gaccggctgc tgcgatccga aggcggcggc gaagatcgac gaggacggca
     1021 cgccgtcctc gatcagcgtg cgggtgacgg tcagcgcgcg ctgggccgag agttcccagt
     1081 tgtcggcgaa gcggctgttg ctgccgcgca ccggcatgtc gtcggtgaag ccgctgacca
     1141 tcagcatctg ttcgctggcg ccgaggtagg cggcgatggg ctgggacagg ctcttcaaca
     1201 gttgcttgcc ttccggctgc aattggtcgg aattgagcgc aaagagcaca ttgccgctga
     1261 tgccgatctt gccgttgtcg agcgtcacgc ggccggaggc gagcgggccg gccagcgcct
     1321 tttccagcgc caggcggcgt tgttcttcct gctggcgttt ctggatttcg gcttcgaggc
     1381 tgctgaccag ttcgagctgc acaccgagca cgccgaccag gatcagcacg aaggcgccga
     1441 gcagcccggc catcaggtcg ccgaagacgg cccagaccgg ggtgctgtgg tcgacgctgg
     1501 cgtcgatgtc ttccatgatc aggcctcgct agcagcgacg cgttgcaggt cgtcgaccat
     1561 cttcttctgc gacaggatgc tgaggtcgat gatttcgcgc gcctgggcga cgtagtaggc
     1621 gagttgctcg tcactgcggg tcagcgactt ggctaggccg ttttcgatgc gttgcaacgc
     1681 ggccatcatc ttgtcgttcg attcggcgaa cagcttgact gcggcgccga aggcttcgcc
     1741 caggctggcc acttcgagcg cgccaccggc gatgccgctg ccgacctcga ccagcttgcc
     1801 ggcttctctc tcgatcctgg cggcgaactg gctgccggcg cgttcgagca gttcggccga
     1861 cgacgcgacg agcgcgtcga tggcgctgcg ctgctcggtc gaggcgtggt tgatggcgtc
     1921 gagcagggcg cccagggttt ccatgatgcg gctgcgctct tcgagcaggc tgttgtcacg
     1981 ggccatgctc gccgacagtt cgtggcgcag ttggccgatg acctcagccg cggccttggg
     2041 cgcttcggcg gcggtgtgca tgaggcgggt gacttcggcg atggtcgtct cggcctggcg
     2101 ctggtgggcg gcgaccagct cgcgggcggt gtcgccgagc gtgcgggtaa tctgttcctg
     2161 ctgcgccagt gtggcgtcgc cgtgttgctg ccattgcgct tcgagcttgc cggccatggc
     2221 ggcgagggca tcggcgaatt gggactgacg ggcctgttcg ccgcttgccc aaccgcgctg
     2281 ccagtcagcc tgcgtggcgc gcatgtcggt gagcagggca gcagtgcgct gggcgaaagt
     2341 gtcggcgagg ctgatttgcg tttgttccag cgcctgcgtc aggcggtcgc tcgcctgttc
     2401 gtgtttagcg agggcgccgg tccaggtttc ggcaaccgtt tgcacggcgc cgtcgaagcg
     2461 ccgggcgatg ccgtcgagct ggccggcgac ctgctcgctg attgcggcct gctgggcctg
     2521 cgtggtctgg gcgaaggcgg catgactggt ggccagttcg cccttcagcg cggcgtgcgt
     2581 accggccacc gagtcgagca aggcttgcga gcgttggccg aaggtgtcgg cgaagacgct
     2641 cagc
//
LOCUS       DAOSDX010000375         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13625_length_2642_cov_3.416312, whole genome shotgun sequence.
ACCESSION   DAOSDX010000375 DAOSDX010000000
VERSION     DAOSDX010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13625_length_2642_cov_3.416312"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..857
                     /locus_tag="PLN02_08765"
     CDS             237..857
                     /locus_tag="PLN02_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52455.1"
                     /translation="MNVDDAGTLAKGDMKIEGVLGKDDRERSAEALFGFSFIEHLEME
                     IGLARATDHATDPDSRARGVGFGAKWVPYQNDTGWSLGARVDVGRTRIHDRETPEKFT
                     ERSWSVSGLASYRWEGGRVLHLNLGSARTRAQGESETLGTWGIGYEYPLLDRLQLTME
                     IFGEEHARPDKAIGLRYEIVDGLKVSGAVGRGSERNFGQIGVAWEF"
     gene            886..1128
                     /locus_tag="PLN02_08770"
     CDS             886..1128
                     /locus_tag="PLN02_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOI52456.1"
                     /translation="MHAKSFPLSMANPGLRVQVVSLKGGAGLDRRLTEMGLNIGAELL
                     VRQHEGGGVVVTRGETRFALGCGMAHKIMVAPLVGA"
     gene            1151..1384
                     /locus_tag="PLN02_08775"
     CDS             1151..1384
                     /locus_tag="PLN02_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOI52457.1"
                     /translation="MTTLGELQIGDHARVIGYGEAGGAYRRKLLSMGLTPGVEISITR
                     VAPMGDPVELKVRGCQLSLRKEEAAGLNVEKLG"
     gene            1381..>2642
                     /gene="feoB"
                     /locus_tag="PLN02_08780"
     CDS             1381..>2642
                     /gene="feoB"
                     /locus_tag="PLN02_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOI52458.1"
                     /translation="MKPEITIALVGNPNCGKTTLFNALTGAKQRVGNWPGVTVEKKSG
                     EYRHAGRQVEVIDLPGTYSLDVVDREVSLDERVARDFVHTGEADLVINIVDASNLERN
                     LYLTTQLAEMGLPLLLVLNMTDVAAAKGMQVDADALARQLGFPVVPVVASAGTGIDAL
                     KTAIVKAADAPSFSHGLVTYDSALETAIERLAQRIATEMADSRVTPRWLAARLLEGDD
                     LARQQAGPALAATAREAATAFGDDLDILLADARYGSANRIANGCVRVTGKVSRDLTDR
                     IDRVVLNRVLGIPIFLLMMYLMFMFTINIGGAFIDFFDQFTGALLVDGFGAWLSAVGT
                     PAWLTVLLAKGIGGGIQTVATFIPVIGFLYLFLSLLEGSGYMARAAFVMDRFMRWVGL
                     PGKSFVPLIVGFGCNVPAIMATRTLENRR"
BASE COUNT          485 a          920 c          815 g          422 t
ORIGIN      
        1 tacgaaagcg ttgccgaaac catgagcgca gcgcgccgcg ccggcctcgg caagatcggc
       61 ttcgtcaccc aacccggaat atccaacccc taaccctcgc ttccccactt agccgaaaac
      121 atcggaaatc caccatgaag acccccagcc aagctttact aaaaagccca gccaggttca
      181 ctctcctagc tgccaccgca ttcctgatca ctgccccggc gcatgccgaa ggaccgatga
      241 acgtcgatga cgccggcacc ctcgccaagg gcgacatgaa gatcgaaggc gtgctcggca
      301 aggacgaccg cgaacgcagc gccgaagcgc tgttcggctt cagtttcatc gagcatctgg
      361 aaatggaaat cggcctcgcc cgtgccaccg atcatgccac cgacccggac agccgggcgc
      421 gcggcgtcgg cttcggcgcc aagtgggtgc cctaccagaa cgacaccggc tggtcgctcg
      481 gtgcgcgcgt cgatgtcggc cgtacccgca tccacgaccg cgagacgccg gagaaattca
      541 ccgagcgtag ctggtcggtc agcggcctcg cttcctaccg ttgggaaggt ggccgggtcc
      601 tgcacctcaa tctgggcagc gcccgcaccc gcgcccaggg cgaatcggaa accctcggca
      661 cctgggggat cggctacgaa tatccgctgc tcgaccgact gcaactgacg atggaaatct
      721 tcggcgagga acacgcccgc ccggacaagg cgatcggcct gcgctacgag atcgttgacg
      781 ggctcaaggt ctccggcgcc gtcggccggg gcagcgaacg caacttcggc cagatcggcg
      841 tggcctggga attctgacac caccattcaa ttcgaggaga aacgcatgca tgccaagtcc
      901 tttccgctca gcatggccaa ccccggcctg cgggtccagg tggtttccct gaaaggcggc
      961 gcgggactgg accgccgcct gaccgagatg gggctcaaca tcggcgccga actgctggtc
     1021 cgccaacacg aaggcggcgg cgtcgtggtg acgcgcggcg aaacgcgttt cgcgctgggc
     1081 tgcggcatgg cgcacaagat catggtcgcc ccgctggtcg gggcctgaca gacaaacagg
     1141 gagcaggaaa atgacaacac tgggcgaact ccagatcggc gatcacgcca gggtgatcgg
     1201 ttacggcgaa gccggcggtg cctaccgccg caagctgctc agcatggggc tgacgcccgg
     1261 cgtcgagatc agcatcacgc gggtggcgcc gatgggcgac ccggtcgaac tcaaggtgcg
     1321 tggctgccag ctttcgctgc gcaaggaaga agcggccgga ctgaacgtgg agaaactggg
     1381 atgaaaccgg aaatcaccat cgccctggtc ggcaatccga actgcggcaa gacgacgctg
     1441 ttcaacgccc tgaccggtgc caagcaacgg gtcggcaact ggccgggggt caccgtcgag
     1501 aagaagagcg gcgaataccg tcacgccggt cgtcaggtcg aagtcatcga cctgcccgga
     1561 acttactcgc tggacgtcgt cgaccgtgaa gtttcgctcg acgagcgcgt tgcgcgcgat
     1621 ttcgtccata ccggcgaggc cgatctggtg atcaacatcg tcgatgcctc gaacctcgaa
     1681 cgcaacctct acctgaccac gcaactggcc gaaatgggcc tgccgttgct gctcgtcctc
     1741 aacatgaccg acgttgccgc cgccaagggc atgcaggtcg atgccgacgc cctcgcccgc
     1801 cagctcggct tcccggtggt gccggtggtc gcctcggccg gcaccgggat cgacgcgctg
     1861 aagacggcga tcgtcaaggc tgccgacgcg ccgtccttca gccacgggct ggtcacctac
     1921 gacagcgcgt tggaaacggc gatcgagcgc ctggcgcaac gcatcgccac cgaaatggcc
     1981 gattcccgcg tgacgccgcg ctggctcgcc gcccgcctgc tcgaaggcga tgatctcgcc
     2041 cgccagcaag ccggcccggc gctcgccgcg acggcgcgcg aggccgccac cgcgtttggc
     2101 gacgacctcg acatcctgct cgccgatgcc cgctacggca gcgccaatcg cattgccaac
     2161 ggctgcgtgc gcgtcaccgg caaggtcagc cgcgacctca ccgaccgcat cgaccgcgtc
     2221 gtcctcaacc gcgtgctcgg catcccgatc ttcctgctga tgatgtacct gatgttcatg
     2281 ttcaccatca acatcggcgg cgccttcatc gatttcttcg atcaattcac cggcgcgctg
     2341 ctggtcgacg gttttggcgc atggctcagc gccgtcggca ccccggcctg gctcaccgtg
     2401 ctgctcgcca agggcatcgg cggcggcatc cagacggtcg ccaccttcat cccggtgatc
     2461 ggcttcctct acctcttcct gtcgctgctc gaaggctccg gctacatggc gcgcgccgcc
     2521 ttcgtcatgg accgcttcat gcgctgggtc ggtctgcccg gcaagtcctt cgtgccgctg
     2581 atcgtcggtt tcggctgcaa cgtgccggcg atcatggcca cccgcacgct ggaaaaccgc
     2641 cg
//
LOCUS       DAOSDX010000376         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13647_length_2639_cov_3.159830, whole genome shotgun sequence.
ACCESSION   DAOSDX010000376 DAOSDX010000000
VERSION     DAOSDX010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13647_length_2639_cov_3.159830"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLN02_08785"
     CDS             complement(<1..248)
                     /locus_tag="PLN02_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI52459.1"
                     /translation="MPTQAKHAAPDGLHTFPRLLMHHATTRPQAPAMREKYLGIWQTW
                     TWSDVAERVRALACGLAALGLKRGDNLAIIGDNRPHLY"
     gene            420..1094
                     /locus_tag="PLN02_08790"
     CDS             420..1094
                     /locus_tag="PLN02_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI52460.1"
                     /translation="MKNAEELLRRSQWGQALSEEEMQRALAGTVERSFAAGAFICMKG
                     EAVTHWMGIVDGLGKMASHWTSGKTTSLTGISSGAWFGEGSLLKEEPRRYDVMAIRDT
                     RVAFMSRGTFLWLLDHSIAFNRFMITQLNERLGQFIGMMENERMLDIDTRIARVLAAL
                     FNPVLYPGTNRLLQISQEELGYLAGVSRQRANQGLKVLEEAGLVRSEYGGINVIDLDG
                     LRSFGE"
     gene            complement(1104..2432)
                     /locus_tag="PLN02_08795"
     CDS             complement(1104..2432)
                     /locus_tag="PLN02_08795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOI52461.1"
                     /translation="MNDHDSLSAALQRNRLNRRQLLWLFGAAALSGCAQSPVGGKSIL
                     VGMSEDDEKAVDKQVAPQQFSQDMGPVQEGEVNRYLSSVGERMDKLTHRPQMPYSYRV
                     LNANHVNAYTFPGGAMGVTRGIMVDLHNEAELGALLGHEMGHVNARHAAQRQGQAMVA
                     QVAVAGASIAASSTEWGGLVAVGGQLGASALLSSYSRDNEREADALGQEYMVRAGYPA
                     SGMTGLHQLLVDQQKEAPGLLQTMFATHPMNVERRDTARRLAEGKYAASLKRDPGRER
                     FMDNTASLRRIKPTIESCQKGEVAMADKQYGKAEGQFREALKKTPRDYPANLLMAQCL
                     QAQDRDRDALGYAESAKAIYPQEAQAHKLVGVLALGLKDPAAAYAGLDRYDRLLPGDA
                     GIGFLKGVALEGMGKRQEAAQQYAGYLRNNRQGKAAEYAYSRLKGWGYAK"
     gene            2431..>2639
                     /locus_tag="PLN02_08800"
     CDS             2431..>2639
                     /locus_tag="PLN02_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52462.1"
                     /translation="MAFSLRKQTGFGILLEKPPWGQGKPGGAPRLSGPGLPPHIALHW
                     TDPAFHLADLPTGPEFETALLVRLGV"
BASE COUNT          399 a          907 c          849 g          484 t
ORIGIN      
        1 atgtacaggt gcggccggtt gtcgccaatg atcgccaggt tgtcgccgcg cttgaggccc
       61 agtgccgcca ggccgcaggc cagcgcgcgg acgcgctcgg caacgtcgga ccaggtccag
      121 gtctgccaga taccgaggta tttctcgcgc atcgccggcg cctgtggccg cgtcgtcgca
      181 tgatgcatga gcagccgcgg gaaggtgtgc agaccatccg gcgcggcgtg tttcgcctgc
      241 gtgggcataa attgtctcct ccgccgcgac tcatcgttcg cggtcatgtg tcattaggtt
      301 cattgtggcg ccggctttct tacaccagtc ttgcagacgc cgccttttcc tgacctgcgg
      361 gccagtgtaa gcacaggcgc ggcgtacaat tgtcgtctgg cagacaatcc ggataaaaaa
      421 tgaagaacgc tgaggaattg ctgcggcgct cccagtgggg acaggcgctc agtgaggagg
      481 agatgcagcg cgcgctggcc ggcaccgtcg aacgcagctt cgcggccggc gccttcatct
      541 gcatgaaggg cgaggcggtc acgcactgga tgggcatcgt cgacgggctg ggcaagatgg
      601 ccagccactg gacctcgggc aagaccacgt cgctgaccgg catcagcagt ggcgcctggt
      661 tcggcgaagg ctcgctgctc aaggaggaac cgcgccgcta cgacgtcatg gcgatccgcg
      721 acacccgcgt cgccttcatg agccgcggca ccttcctgtg gctgctcgac cacagcatcg
      781 ccttcaaccg cttcatgatc acccagttga acgaacgcct cggccagttc atcggcatga
      841 tggaaaacga gcgcatgctc gacatcgaca cccgcatcgc ccgcgtcctg gcggcgctgt
      901 tcaacccggt gctctacccc ggcaccaacc gcctgctgca gatttcccag gaagaactcg
      961 gctacctcgc cggcgtctcc cggcaacgcg ccaaccaggg cctcaaggtg ctcgaggaag
     1021 ccggcctggt gcgcagcgaa tacggcggca tcaatgtcat cgacctcgac ggcctgcgca
     1081 gtttcggcga gtaagcgctc accctacttg gcgtagcccc aacccttgag tcggctgtag
     1141 gcgtattcgg cggccttgcc ctggcggttg ttgcgcaggt agccggcgta ctgctgcgcc
     1201 gcttcctggc gcttgcccat gccttccagc gccaccccct tgaggaagcc gatgccggca
     1261 tcgcccggca gcagccggtc gtaacggtcg agtccggcgt aagcggcggc cgggtccttg
     1321 aggccgagcg ccagcacgcc gaccagcttg tgcgcctgcg cttcctgcgg atagatcgcc
     1381 ttcgccgatt cggcgtagcc cagcgcgtcg cggtcgcgat cctgcgcctg caggcattgc
     1441 gccatcagca ggttggccgg atagtcgcgc ggtgttttct tcagggcctc gcggaactgc
     1501 ccttcggcct tgccgtactg tttgtcggcc atcgccacct cgcccttctg gcaggattcg
     1561 atggtcggct tgatgcggcg cagcgacgcc gtgttgtcca tgaagcgctc gcgcccgggg
     1621 tcgcgcttca ggctcgccgc gtacttgccc tcggccagcc gacgcgccgt gtcgcgccgt
     1681 tcgacgttca tcggatgggt cgcgaacatg gtctgcagca gaccgggcgc ttccttctgc
     1741 tggtcgacca gcaactggtg caggccggtc atgccgctcg ccggataacc ggcgcgcacc
     1801 atgtattcct ggcccagggc gtcggcttcg cgctcgttgt cgcgcgaata gctggagagc
     1861 agcgcgctgg cgcctagctg gccgccgacc gcgaccagcc cgccccattc ggtggacgag
     1921 gcggcgatgc tggccccggc caccgccacc tgggcgacca tcgcctgccc ctggcgctgc
     1981 gccgcgtgcc gcgcattgac gtgtcccatt tcgtggccga gcagcgcgcc cagttcggct
     2041 tcgttgtgta gatcgaccat gatcccgcgc gtcaccccca tcgccccgcc ggggaaggtg
     2101 taggcgttga cgtggttggc gttgagcacg cggtaggaat acggcatctg cggccggtgc
     2161 gtcagcttgt ccatgcgttc gccgaccgaa ctcaggtagc gattgacctc gccctcctgc
     2221 accggcccca tgtcctgcga gaactgctgc ggcgccacct gcttgtccac cgccttctcg
     2281 tcgtcctcgc tcatgccgac caggatcgac ttgccaccga ccggcgactg cgcgcagccg
     2341 gacaatgccg cggcgccgaa cagccagagc agctggcggc ggttgaggcg gttgcgctgc
     2401 agcgcggcac tcagggaatc gtgatcgttc atggcgttct ccttgcgaaa acagacaggc
     2461 ttcggaattt tgctggaaaa accgccttgg ggacagggca agcccggagg tgcgccgcgg
     2521 ctctccggcc ccgggctacc gccccatatc gccctccact ggactgatcc ggctttccac
     2581 cttgctgacc tgccgacggg cccggagttc gaaactgcgc tgctcgtccg tctcggggt
//
LOCUS       DAOSDX010000377         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13664_length_2636_cov_4.171251, whole genome shotgun sequence.
ACCESSION   DAOSDX010000377 DAOSDX010000000
VERSION     DAOSDX010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13664_length_2636_cov_4.171251"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..579)
                     /locus_tag="PLN02_08805"
     CDS             complement(274..579)
                     /locus_tag="PLN02_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52463.1"
                     /translation="MQGVDFYQAELCGALDEGLMSEVMHRNSASMIQVGKLMGDIGSL
                     RSSLAESVGILLLVASGFGFCSVLFSAPDGRAFLLAEPLGSAVDRRAYFLLVSPSRK"
     gene            633..>2636
                     /locus_tag="PLN02_08810"
     CDS             633..>2636
                     /locus_tag="PLN02_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOI52464.1"
                     /translation="MSKHVALSHVTKYKYDRLINLGPQVIRLRPCPHSRTRILSYSLK
                     VGPDQHFINWQQDPQGNYLARLVFPEKTREFSFEVDLVAEMSVINPFDFFLEPHAEEF
                     PFDYEAWQRHELTPYLYKVAGGELFDKYVAAIPREKKRSVDFLVGLNARLQQEIGYTI
                     RMKPGVQTPEETLSKKTGSCRDSAWLLVQILRHLGLAARFVSGYLIQLTADVKSLDGP
                     SGPDADFTDLHAWAEVYLPGAGWIGLDPTSGLFAGEGHIPLACTPEPASAAPVTGGLD
                     ECETDFEHHMQVTRIWEAPRVTKPYTDEQWVEIEGLGHQIDDELLRMDVRLTQGGEPT
                     FVSADDPDGAEWNTAAMGPTKRLLAADLFHRLREKYAPNGLMHFGQGKWYPGEQLPRW
                     SLNCFWRKDSEPVWNNPALYADERKDYGVSAEQAGQFLKRVAERLGVTPDYVFPAYED
                     VYYYMWRERRLPGNVDPFDSRVDDALERARLMKVFTQGMTHVVGHTLPIMKNVRNEWQ
                     TGPWFLRAERCYLFPGDSAMGYRLPIDSQPWTAQSDYPYVNVPDAETATDPLASYAAL
                     RARVSGEAGARLPQMQARRSGAPGADGLAEGEGRTRPWEKPTDTVPGFKESAAWITRL
                     AMCAEPRDGKLYIFMPPTQTLDDYLEIVTAVEATAEEMRLPVIL"
BASE COUNT          501 a          901 c          795 g          439 t
ORIGIN      
        1 ccgacttccc ccggcccttc tccgttgctc ggcgctccac atgggggccc cgaaaagcgt
       61 ctctgctgaa tggccaaggt ttaaatcctg agtgtttccc ttccgctcgg tttgccgggc
      121 cccttgggag gcgccgagca acgcaggcgt tggcggataa agggcgagca ctgtctgagg
      181 ggcaaagccc cgagttgcgc agcccccgcc aacgacgagt agcgcagggc acccgcgtag
      241 cgggcgccga cctagggtcg ccttcttctt tggctacttt ctgcttggcg aaacaagaag
      301 aaagtacgcc cgccggtcaa cggcggaacc cagcggctca gcaagcaaaa acgcccgccc
      361 gtcaggcgcg gaaaacagca ccgaacaaaa accaaatcca ctcgccacca gcagcaaaat
      421 gccaacactt tcagcaagtg acgacctcaa tgaaccaata tcgcccatca gcttcccaac
      481 ctggatcatc gatgcactat tgcggtgcat tacctccgac atcaagcctt catcaagtgc
      541 gccgcacaat tccgcttgat aaaaatcaac gccttgcaag ctggttcgcc tcttgctttt
      601 aagctgccaa acaaactccc cggagcctac ccgtgtccaa acacgtcgca ctgagccacg
      661 tcaccaagta caaatacgac cgtctgatca atcttggccc gcaggtcatc cgcctgcggc
      721 cgtgtccgca ttcgcgcacg cgcatcctct cctattcgct gaaggtcggt ccggaccagc
      781 atttcatcaa ctggcagcag gacccgcagg gcaattacct ggcgcgcctg gtgttcccgg
      841 agaagacccg cgagttcagc ttcgaggtcg atctggtcgc cgagatgtcg gtgatcaacc
      901 ccttcgactt cttcctcgag ccgcatgccg aggagtttcc gttcgattac gaggcctggc
      961 agcgccatga actgacgccc tacctgtaca aggtggccgg cggcgagctg ttcgacaagt
     1021 acgtggcggc gattccgcgc gagaaaaagc gcagcgtcga ttttctggtt ggcctgaatg
     1081 cccggttgca gcaggagatc ggctacacga tccggatgaa accgggggtg cagacgccgg
     1141 aagaaacact gtccaagaag accggttcct gccgcgattc ggcctggctg ctggtgcaga
     1201 tcctgcgcca tctgggcctg gcggcgcgtt tcgtttccgg ttacctgatc cagctcaccg
     1261 ccgacgtcaa gtcgctcgac ggcccgtccg gcccggacgc cgatttcacc gacctgcacg
     1321 cctgggccga ggtctatctg ccgggtgccg gctggatcgg cctcgacccg acttccggcc
     1381 tgttcgctgg cgaaggccac attccgctcg cctgcacgcc ggaacccgct tccgccgcgc
     1441 cggtgaccgg cggcctggac gagtgcgaga ccgacttcga gcaccacatg caggtcaccc
     1501 gaatctggga ggcgccgcgc gtcaccaagc cgtacacgga tgaacagtgg gtcgagatcg
     1561 agggcctcgg ccatcagatc gacgacgaac tcctgcgcat ggacgtccgc ctgacccagg
     1621 gcggcgagcc gaccttcgtc tcggccgacg accccgacgg cgctgaatgg aacacggcgg
     1681 cgatggggcc gaccaagcgc ctgctggccg ccgatctttt ccaccgcctg cgcgaaaaat
     1741 acgcgcccaa cggcttgatg catttcggcc agggcaagtg gtatcccggc gaacagttgc
     1801 cgcgctggtc gctgaactgc ttctggcgca aggacagcga gccggtctgg aacaatccgg
     1861 cgctgtacgc cgacgagcgc aaggattacg gcgtcagcgc cgaacaggcc gggcaattcc
     1921 tcaagcgcgt cgccgagcgc ctgggcgtca cgccggacta tgtcttcccg gcctacgagg
     1981 acgtctatta ctacatgtgg cgcgagcgcc gcctgccggg caacgtcgat cccttcgatt
     2041 cgcgcgtcga tgacgcgctt gaacgcgccc ggctgatgaa ggtgttcacc cagggcatga
     2101 cccatgtcgt cgggcacacg ctgccgatca tgaagaacgt ccgcaacgaa tggcagaccg
     2161 ggccgtggtt cctgcgcgcc gagcgttgct acctgttccc cggcgactcg gcgatgggct
     2221 accggctgcc gatcgactcg caaccgtgga cggcgcagag cgattacccc tacgtcaatg
     2281 tgccggacgc tgaaacagcg accgatccgc tggccagtta cgcggcgctg cgcgcccgcg
     2341 tcagcggcga agccggcgcc cgcctgccgc agatgcaggc gcgacgcagc ggcgcgcctg
     2401 gcgcggacgg actggccgag ggcgagggcc ggacccggcc gtgggagaag ccgaccgaca
     2461 cggtccccgg tttcaaggaa tccgccgcct ggatcacccg cctggcgatg tgcgccgagc
     2521 cgcgcgacgg caagctctac atcttcatgc cgccgaccca gacgctcgac gactacctgg
     2581 agatcgtcac cgccgtcgag gcgaccgccg aggaaatgcg cctgccggtc atcctc
//
LOCUS       DAOSDX010000378         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13675_length_2634_cov_3.485072, whole genome shotgun sequence.
ACCESSION   DAOSDX010000378 DAOSDX010000000
VERSION     DAOSDX010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13675_length_2634_cov_3.485072"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PLN02_08815"
     CDS             <1..784
                     /locus_tag="PLN02_08815"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOI52465.1"
                     /translation="QFAPEIVAACRKAVAGAQADLTFLRVDKAAFAACPTDSIDYAVM
                     EKTADAAVVALDAGWSDVGCWSALWDLSAKDAAGNAVLGDVILQDCAGSYVHASNRLV
                     AAVGVRDLIVIETKDAVLVASKDRVQEVKQVVERLKADGRPEHDKHREVYRPWGIYDS
                     IENGVRYQVKRITVRPGAKLSVQMHHHRAEHWIVVSGTARITKGEQTYLLTENQSTYI
                     PVGELHALENPGKIPLELIEVQSGSYLGEDDIVRLEDRYGRT"
     gene            925..2202
                     /locus_tag="PLN02_08820"
     CDS             925..2202
                     /locus_tag="PLN02_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52466.1"
                     /translation="MHNIPDWPAFQKLLNGREVYIYGGNVEGVGVSRMISSHYRPTRG
                     FIDTRQFNHGFLRGVPVVHPAAFSDSPAAAFIVICTKHRETRALAKKFCLERGYVEGR
                     DFVFNSALCRYFPTIETVGICNLRCITCDMGIPGANHNQKMMRLETFSAILDKLKAEI
                     PFLNSLALYLWGEPLLHPEVGEMVRACHARGIATEFSSNLNNIRHLDSFIAADPDILI
                     VTSSGFGDNYEITHTGGDFEKFRNNCIQLRQMIDKHGAETFVKYHYCVYNNNQGEEML
                     AARRFAESLNFQFVPILANIFPGKVHDYVVLGTPLPDVMLEANKYLLYDIHDQIQWAQ
                     AQRDKHCPVIKAFPTIRWDGRVMQCCNMTQPFVGDATYLETSMSELLELRESSGFCQR
                     CMAHGMHRVFDVNGREDKRMTTIASSKNDSPKA"
     gene            2205..>2634
                     /locus_tag="PLN02_08825"
     CDS             2205..>2634
                     /locus_tag="PLN02_08825"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOI52467.1"
                     /translation="MYFSYCPTKKIFGDNAVPALAREIGDAGRIALIASASAMNAPGL
                     AEALAPLDARICLRQISHAAGEPDYLAVGALADTLRERQVTLLLCVGGASVIDLAKAA
                     AVCAHFPGNADEKWAQLLASPFDTPALPIAVISSLPGTSSE"
BASE COUNT          517 a          921 c          786 g          410 t
ORIGIN      
        1 gcagttcgcg ccggagatcg tcgccgcctg ccggaaagcg gtcgccggcg cgcaggcgga
       61 tctgaccttc ctgcgggtgg acaaggcggc gttcgcggcc tgcccgacgg attcgatcga
      121 ctacgcggtc atggaaaaaa ccgccgacgc cgcggtcgtc gccctcgacg ccggctggag
      181 cgacgtcggc tgctggtcgg cgctgtggga cctgagcgcc aaggacgccg ccggcaacgc
      241 cgtccttggc gacgtcatcc tgcaggactg cgccggctcc tacgtccatg ccagcaaccg
      301 cctggtggcg gcggtgggcg tcagggacct gatcgtgatc gagaccaagg atgcggtcct
      361 ggtcgccagc aaggaccgcg tgcaggaggt gaagcaggtg gtcgaacggc tcaaggccga
      421 cggccgcccc gaacacgaca agcaccgcga ggtctatcgt ccctggggca tctacgacag
      481 catcgagaac ggcgtccgct accaggtcaa gcgcatcacg gtgcggccgg gcgccaaact
      541 gtcggtgcag atgcaccacc accgcgccga acactggatc gtcgtcagcg gcaccgcccg
      601 gatcaccaaa ggcgagcaga cctacctgct gacggagaac cagtccacct acattccggt
      661 cggcgaactg cacgccctgg aaaacccggg caagattccg ctcgaactga tcgaggtgca
      721 gtcgggctcc tacctgggcg aagacgacat cgtcaggctg gaagaccgtt atggccggac
      781 atgaagacgc cgaccgcgcg tcggcgctcg tatatcatgc tgaaacggat tcacccagcc
      841 aagcttgcgg gcgcggcggc gccaccgttc cagaacatca gcgtttagcg gcctgaccgc
      901 gacgcttcag ccgaagacac gacgatgcac aatatcccag actggccggc cttccagaag
      961 ctgctgaacg gccgcgaggt ttacatctat ggcggcaacg tcgaaggcgt cggcgtgtcg
     1021 cggatgatct ccagccacta ccggccgacc cgcggcttca tcgacacccg gcagttcaac
     1081 cacggcttcc tgcgcggcgt gccggtggtg catccggcgg cattcagcga cagcccggcc
     1141 gccgccttca tcgtcatctg caccaagcac cgggaaaccc gcgcgctggc gaaaaaattc
     1201 tgcctcgaac gcggctatgt cgagggacgg gatttcgttt tcaattcggc gctctgccgc
     1261 tatttcccga ccatcgagac ggtcggcatc tgcaacctgc gctgcatcac ctgcgacatg
     1321 gggattcccg gcgcgaacca caaccagaag atgatgcgcc tggaaacctt cagcgccatc
     1381 ctcgacaagc tgaaggcgga aatccccttc ctcaactcgc tggcgctgta tctgtggggc
     1441 gagccgctgc tccatcccga ggtcggcgaa atggtgcgcg cctgccacgc gcggggtatc
     1501 gccaccgagt tctcctcgaa cctgaacaat atccgccatc tcgattcctt catcgccgcc
     1561 gacccggaca tcctgatcgt caccagctcc ggtttcggcg acaactacga gatcacccac
     1621 accggcggcg acttcgagaa gttcaggaac aactgcatcc agctgcggca aatgatcgac
     1681 aagcacggcg ccgagacctt cgtgaagtat cactactgcg tctataacaa caaccagggc
     1741 gaggaaatgc tcgccgcccg ccgcttcgcc gaatcgctga acttccagtt cgtgcccatc
     1801 ctcgccaaca tcttccccgg caaggtgcac gactacgtgg tgctcggcac cccgctgccc
     1861 gacgtgatgc tggaagccaa caagtacctg ctctacgaca tccacgacca gatccagtgg
     1921 gcgcaagccc agcgcgacaa gcactgcccg gtgatcaagg cctttccgac gatccgctgg
     1981 gacggtcggg tcatgcagtg ctgcaacatg actcagccct tcgtaggcga cgcgacctac
     2041 ctcgaaacca gcatgtccga actgctcgaa ctgcgcgaat cgtccggctt ctgccagcgc
     2101 tgcatggcgc acggcatgca ccgggtattc gacgtcaacg ggcgcgagga caagagaatg
     2161 accacgatcg ccagcagcaa gaacgactcg cccaaagcct gaccatgtac ttcagttact
     2221 gcccgaccaa gaaaatcttc ggcgacaacg cggtcccggc gctcgcccgg gaaatcggcg
     2281 acgccggcag aatcgccctg atcgccagcg ccagcgccat gaacgcgccg gggctggcgg
     2341 aagcgctcgc cccgctcgac gccaggatct gcctgcgcca gatctcccat gccgccggcg
     2401 aaccggatta cctcgccgtc ggcgccctcg ccgacaccct gcgcgagcgc caggtgacgc
     2461 tgctgctgtg cgtcggcggc gcctcggtga tcgacctggc caaggcggcg gcggtctgcg
     2521 cccacttccc cggcaatgcc gacgaaaaat gggcgcaact gctggcctcg cccttcgaca
     2581 cgccggccct gccgatcgcc gtcatcagca gcctgcccgg cacctcctcg gaat
//
LOCUS       DAOSDX010000379         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13711_length_2629_cov_3.067988, whole genome shotgun sequence.
ACCESSION   DAOSDX010000379 DAOSDX010000000
VERSION     DAOSDX010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13711_length_2629_cov_3.067988"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLN02_08830"
     CDS             complement(<1..709)
                     /locus_tag="PLN02_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI52468.1"
                     /translation="MALDLFLEELRRPAAGEARQQRLYRLLKQAIVDGRLGAGLRLPA
                     SRRLAVDAGMARNCVLHAYQQLLAEGFLVADRGGTRVASLPTPSRLLPLVPASGVLSR
                     RAATLSASSRGEPTLPFAPGVPDLNAFPWPLWSRLLQDAWRAVGARHLAYAEVGGEPV
                     LRQAIASFLGARRGVACTAAQVFILPGAQAALDACARLLADPGDLAWVEEPGYPVARN
                     TLLAAGLTVQSVAVDGQG"
     gene            795..1457
                     /locus_tag="PLN02_08835"
     CDS             795..1457
                     /locus_tag="PLN02_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOI52469.1"
                     /translation="MKPDAPSPRTQIRRKPQRGHYDFATIAAIVDAAQICQIAFNEGG
                     SVHCLPTACWRVGNHLYIHGASNSRLTRTLSGSDCSVCIAHLDGLVLARSAFHHSMNF
                     RSVVIYGRFIAVDDPEEKKDVLAAFVEHISPGRSALVRPASTAEVAGTAVLRLSLDEA
                     AAKIRNWGVDDDAEDLEIPVWAGVLPLQVVAGTAIPEAGCAEMAKPAHRFPQAAEYES
                     AP"
     gene            complement(1514..1678)
                     /locus_tag="PLN02_08840"
     CDS             complement(1514..1678)
                     /locus_tag="PLN02_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HOI52470.1"
                     /translation="MAWNLLLTSDIGLLSLFTILFILVMGVYIGRYASKQIAKENSAS
                     HGAPPHQGAH"
     gene            complement(1902..>2629)
                     /locus_tag="PLN02_08845"
     CDS             complement(1902..>2629)
                     /locus_tag="PLN02_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HOI52471.1"
                     /translation="HPQPDGRGIHTRTLQVLEQSIAQWVQSRDVLVLMVPSVLQDGIL
                     MRSNIRLRDYAEYLDGLILQGGADVSPLAYGETPLQPAWAGDPVRDAYELELLHEFME
                     AGKPVLGICRGMQLINVALGGSLYQDLPTQFPSDIAHEHEDYDRHAHPVRFSPEGHFI
                     RWFAGGEGGQVVSIHHQAIHRLGNDVVIEAEAADGIIEAIRWKGRSFVCGVQWHPEFH
                     HHGDGGLLDCAPLLDAFLDAARR"
BASE COUNT          501 a          864 c          843 g          421 t
ORIGIN      
        1 tgccctggcc gtccaccgcc accgactgca ccgtcagccc ggcggcgagc agcgtgttgc
       61 gggcgaccgg gtagcctggc tcctcgaccc aggcaaggtc gccgggatcg gccagcaggc
      121 gggcgcaggc gtcgagcgcc gcctgggcac cgggcaggat gaagacttgc gccgccgtgc
      181 aggcaacgcc gcggcgggcg ccgaggaaac tggcgatggc ctgacgcagc accggctcgc
      241 cgccgacttc ggcgtaagcc agatgacggg cgccgaccgc acgccaggcg tcctgcagca
      301 gacgcgacca gagcggccag ggaaaggcgt tgaggtcggg aacgccgggc gcgaacggca
      361 gtgtcggctc gccgcggctg ctcgccgaca gcgtggcggc gcggcgcgac aggacgccgg
      421 atgccggcac aagcggcagc agccttgacg gcgtcggcaa gctggccacg cgcgtaccgc
      481 cgcgatcggc gacgagaaaa ccttcggcaa gcagttgctg gtatgcgtgc aacacgcagt
      541 tgcgcgccat gcccgcgtcg accgccagac gacggctggc cggcagacgc aggccggcgc
      601 cgaggcgacc gtcaacgatc gcctgcttga gcaggcggta cagccgctgc tggcgtgcct
      661 caccggcggc aggccgacgc agttcttcga ggaagagatc gagcgccatc tggctccatc
      721 attgagtggt ttcttggctc catcgatggt gccatagccg ggctatgctg accggacttt
      781 ttcaggagaa catgatgaaa ccggacgccc ccagcccgcg aacgcaaatc cgccgcaagc
      841 cgcaacgcgg ccactacgac ttcgccacca tcgccgccat cgtcgatgcg gcgcaaatct
      901 gccagatcgc cttcaacgag ggaggcagcg tgcactgcct gccgactgcc tgctggcgcg
      961 tcggcaatca cctgtacatc cacggtgcca gcaactcgcg gctaacccgg accctgagcg
     1021 gcagcgactg ctcggtctgc atcgcccatc tcgacggcct ggtgctggcc cgctcggcct
     1081 ttcaccattc gatgaatttc cgctcggtgg tcatctacgg tcgcttcatc gcggtcgatg
     1141 acccggaaga gaagaaagac gtgctcgccg cctttgtcga gcacatcagc ccggggcgct
     1201 cggcgctggt acgcccggcc agtacggccg aagtcgccgg caccgcggtg ctgcgtctga
     1261 gcctcgacga agcggcagcg aagattcgca actggggcgt tgacgacgac gccgaggacc
     1321 tggagattcc cgtctgggcc ggcgtgcttc cgctacaggt ggtcgccgga acggccatcc
     1381 cggaggcagg ttgtgccgaa atggcgaaac cggcgcatcg ctttccacag gctgccgagt
     1441 acgaatcagc gccgtaaacg aatttcgccg gccaagggcc ggcgaatcag acttgaactt
     1501 ctctttgcgc ggctcagtgg gcaccctgat gcggcggcgc gccatgagaa gcgctgtttt
     1561 ccttggcgat ctgcttcgat gcgtagcgtc cgatatagac gcccatcaca aggatgaaaa
     1621 gaatcgtgaa aagactgagc aaaccgatgt cagaagttaa gagcagattc caggccatga
     1681 acgttctccc tcgaattttt tgaaagtgct gaatcagccc tcatacgaac gcaataattg
     1741 cgccagatca aatcagcctc gagggaattt cgcgaaacaa cgccgatgtc cgccagacgg
     1801 tcgttttcgg caatccacga cacccgcgcg agcgtcatga ccgcgcttgc tttgccaaaa
     1861 atggcacaca agcctgccaa ccatgtcaca ccagacaaat cctagcgcct tgccgcgtcg
     1921 agaaatgcat ccagcaaggg ggcgcaatcg agcagaccgc cgtcgccatg gtggtggaat
     1981 tccgggtgcc attgcacgcc gcagacaaag ctgcgccctt tccagcggat cgcctcgatg
     2041 atgccgtccg ccgcctcggc ttcgatcacg acgtcgttgc cgagtcggtg tatcgcctga
     2101 tggtggatcg acaccacctg cccgccttcg ccgccggcga accaacggat gaagtggcct
     2161 tcgggcgaga agcgcaccgg atgcgcgtgg cggtcgtaat cctcgtgttc gtgggcaatg
     2221 tccgatggaa actgggtcgg cagatcctga tacagcgaac cgccgagcgc gacattgatc
     2281 aactgcatgc cgcgacagat gccgagcacc ggtttgcccg cctccatgaa ctcgtgcagc
     2341 agttccagtt cgtaggcgtc gcgcaccgga tcgccggccc acgccggttg cagcggcgtt
     2401 tcgccgtagg ccagcgggct gacgtcggcg ccgccctgca ggatcaggcc gtcgagatac
     2461 tcggcgtagt cgcgcagacg gatgttgctg cgcatcagga tgccgtcctg caggacggag
     2521 ggtaccatca gcaccagcac atcacgcgac tgcacccact gggcaatcga ctgctccagc
     2581 acctgcaagg tccgtgtgtg gatgccgcgg ccgtccggct gcggatgat
//
LOCUS       DAOSDX010000380         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13743_length_2624_cov_3.249124, whole genome shotgun sequence.
ACCESSION   DAOSDX010000380 DAOSDX010000000
VERSION     DAOSDX010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13743_length_2624_cov_3.249124"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLN02_08850"
     CDS             complement(<1..392)
                     /locus_tag="PLN02_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOI52472.1"
                     /translation="MNFQTIAFSASDGIARLTLNRPDKLNSFTGEMHAELRVALDRVQ
                     ADHSIRVLVLSGAGRAFCAGQDLADPDMQAINGQLPDIGNVVEKNYKPLVLRLENLRV
                     PTLAAVNGIAAGAGASLALACDLVVATQS"
     gene            738..1613
                     /locus_tag="PLN02_08855"
     CDS             738..1613
                     /locus_tag="PLN02_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI52473.1"
                     /translation="MLAQFLQFLFSGVTVGATYALAALGFTLIYNASNVINFAQGEFI
                     MLGGMLAVFFAQMGLPLPVVLLLAVLVPAVVGVLVEKLAIEPVKGAETVTLIIITIGA
                     SLVIRGIVQVWLGKNTFSLPAFSGDAPIEILGATLLPQSLWVLGITALVVAGLWYFFN
                     RTLQGKAMLATSFNRTAAELVGINTSWVLFLSFAMSAALGALGGILITPITLTSYDVG
                     IMLGLKGFVAAVVGGLGNGLGAVVGGLLVGILEAMGAGYISSAYKDAIPFVLILLILF
                     FMPRGLFGGKSTDRV"
     gene            1616..2548
                     /locus_tag="PLN02_08860"
     CDS             1616..2548
                     /locus_tag="PLN02_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI52474.1"
                     /translation="MQKNPHLGLGLLAAILVALPFVLPNSFYLDLAVRMAINAIIVIG
                     LNLLIGFAGQISLGHAGFLGIGAYASAVLPTHFGWHPLLALLTGAVASALLAALVARP
                     IFKLKGHYLAMATLGLGIIINIALRNEAQWTGGPDGMAVPAMALFGIEISNDRQWYWV
                     VAALLLVTVWASLNLIDSPFGRALRALHGSEVASQVVGVDVVRYKVSIFVMSAFSASV
                     MGSISAHYIGFISPNFADFFHSIELVTMVVIGGMASVYGSVVGAVLLTALPQALATFE
                     GWETVAFGAILMLCMIFLPRGLVPTLAARFGKGE"
BASE COUNT          365 a          854 c          879 g          526 t
ORIGIN      
        1 gactgcgtgg cgacaaccag gtcgcaggcc agcgcgagcg aggcgccggc gccggcagcg
       61 atgccgttga ccgcggccag ggtcggcacg cgcaggttct ccagacgcag caccagcggc
      121 ttgtagttct tctcgacgac gttgccgatg tccggcagtt ggccgttgat tgcctgcatg
      181 tcggggtcgg ccaggtcctg gccggcgcag aaagcccggc cggcaccgct gaggacgagc
      241 acgcggatgc tgtggtcggc ctgaacgcga tcgagcgcga cgcgcagttc ggcgtgcatt
      301 tcgccggtga agctgttcag cttgtcgggg cggttcaggg tcaggcgggc gatgccgtcg
      361 ctggcggaaa aggcaatggt ctggaagttc attcggttct cctcaagtag tgcagcaagc
      421 gcagcccacg tggggactgg cgcagcactc ggttctattg ggttgctgcc ggcggcggga
      481 cggcggaagc ggtgtcgtag attaatgcat gcgtgccgat gtggtaacag cgcgcgctgg
      541 caatcgtgcc gttgcccttg cggacgacgg cgccggaccc ggctttccgc cgtccgcccg
      601 aggcgacggg gcttgcccgc cgggccatgt aaaacgccgc cgttccggtt gccaacttgg
      661 gtcttcgccc tagacaatgt ggggctattg gaggagagaa caatgaaaat gaccggcctg
      721 cgggcagggg gacgctgatg ctcgcccagt tcctgcaatt cctgttttcc ggggtcaccg
      781 tcggcgccac ctacgcgctg gccgccctcg gcttcacgct gatctacaac gccagcaacg
      841 tcatcaactt cgcgcagggc gagttcatca tgctcggcgg catgctcgcc gtcttcttcg
      901 cgcagatggg gctgccgctg ccggtcgtgc tgctgctcgc cgtcctggtg ccggcggtgg
      961 tcggcgtcct ggtcgagaag ctggccatcg agccggtcaa gggcgccgag acggtgacgc
     1021 tgatcatcat caccatcggc gcttcgctgg tcatccgcgg catcgtccag gtctggctgg
     1081 gcaagaacac cttcagcctg cccgccttct cgggcgacgc gccgatcgag atactcggcg
     1141 ccacgctgct gccgcagagc ctgtgggtgc tcggcatcac cgcgctggtg gtggccggcc
     1201 tctggtactt cttcaaccgc acgttgcagg gcaaggcgat gctcgccacc tcgttcaacc
     1261 ggacggcggc cgaactggtc ggcatcaaca cgagctgggt gctcttcctc agcttcgcca
     1321 tgtcggcggc gctcggcgcg ctcggtggca tcctgatcac gccgatcacc ctgacttcct
     1381 acgacgttgg catcatgctc ggcctcaagg gcttcgtcgc cgccgtcgtc ggcgggctgg
     1441 gcaacggctt gggcgcggtg gtcggcggcc tgctggtcgg cattttggag gcgatgggcg
     1501 ccggctacat ttcgtcggcg tacaaggacg cgattccctt cgtcctcatc ctgctcatcc
     1561 tgttcttcat gccgcgtggc ctgttcggcg gcaaatcgac cgatcgggtg taagcatgca
     1621 aaagaaccct catctcggcc tcgggctgct cgccgccatc ctcgtcgcgc tgcccttcgt
     1681 gctgcccaac agtttctacc tcgacctcgc ggtacggatg gcgatcaatg ccatcatcgt
     1741 catcggtctc aatctgctga tcggtttcgc cggccagatc agcctcggcc acgccggttt
     1801 cctcggcatc ggtgcctacg cctcggcggt gctgccgacc catttcggct ggcatccgct
     1861 gctcgccctg cttaccggcg ccgtcgcctc ggcgctgctg gcggcgctgg tggcgcggcc
     1921 gatcttcaag ctgaaggggc attacctggc gatggcgacg ctcgggctgg gcatcatcat
     1981 caacatcgcg ctgcgcaacg aggcgcaatg gaccggcggc ccggacggca tggcggtgcc
     2041 ggcgatggcg ctgttcggca tcgagatcag caacgaccgc cagtggtatt gggtggtcgc
     2101 cgcgctgctg ctggtcaccg tctgggcctc gctcaacctg atcgattcgc ccttcggtcg
     2161 cgccctgcgc gcgctgcacg gctccgaagt ggcgtcgcag gtggtcggcg tcgacgtcgt
     2221 ccgctacaag gtgtcgatct tcgtcatgtc ggccttctcg gccagcgtga tgggcagcat
     2281 ctcggcgcat tacatcggtt tcatcagccc caacttcgcc gatttcttcc actcgatcga
     2341 actggtgacc atggtcgtca tcggcggcat ggcctcggtc tacggttcgg tcgtcggcgc
     2401 cgtgctgctc actgcgttgc cgcaggcgct ggccaccttc gagggctggg agacggtggc
     2461 cttcggcgcc atcctgatgc tgtgcatgat cttcctgccg cgtggcctgg tgccgacgct
     2521 ggcggcgcgt ttcggcaagg gggagtgaga tgagcctgct ccaggtacgc aacctgtcga
     2581 tccatttcgg cggcgtcaag gcggtgcaga acgtcagctt cgac
//
LOCUS       DAOSDX010000381         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13747_length_2624_cov_2.778902, whole genome shotgun sequence.
ACCESSION   DAOSDX010000381 DAOSDX010000000
VERSION     DAOSDX010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13747_length_2624_cov_2.778902"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="PLN02_08865"
     CDS             complement(<1..1573)
                     /locus_tag="PLN02_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOI52475.1"
                     /translation="MKFSPIIALLVWGVSAFAQSADDSAFIAAREAFRKGDAARLASA
                     VGETRGHPLAIYAENYLLRNRLARNDADGIPAFLARNEGSNVAERLRSDWLTWLAKQG
                     DWPRVESEYAKLLAPDTERTCLQQQARLQRGDRRVLDEAAGYWLSMLEPSEACKPLLD
                     ALVAAGRIDVDAAWQRARRQIEINRPGAAQQTLAYLPDSQVPGTADFDAATKKSMSYL
                     ARQGANWQRQRSNRELAAIAIRYVAVNDPLVAAEQLDKREAQLERSAGGWAWSQIALL
                     GARRHLPEASIWYARAGDAALTDENLQWRVRAALRALNWGQVRDAIQAMPPELANRPE
                     WIYWLGRAYKSGGRTAEADALFAKIAGQAHFYGNLADEELGRQVRPQASATPPSAEEI
                     AAARDNPNLQRALSLFRLDMRTEAVREWNWALREMNDRQLLAAAELARRNQLWDRAIN
                     TADRTRNEHDYSLRFLSPFADSIRPVTRQQALDDAWVYGLMRQESRFITSARSNVGAA
                     GLMQLMPATARWVAKK"
     gene            1588..2166
                     /locus_tag="PLN02_08870"
     CDS             1588..2166
                     /locus_tag="PLN02_08870"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOI52476.1"
                     /translation="MTGRFEDSTLWRRDLRRDMVARREMLGEKERARLSAAIVGWLRK
                     HFPPPASCAFCWPIKHEPDVRAIIDEWTGRGTRAALPVVVAEATPLQFREWRAGDRLQ
                     ADRYGIPTPVDGDWLQPELILLPLNGFDAAGYRLGYGGGYFDRTLAALTPRPLTVGVG
                     FEINRLDDIRPEVHDQRLDWIVTEAGAFKVAG"
     gene            complement(2150..2437)
                     /locus_tag="PLN02_08875"
     CDS             complement(2150..2437)
                     /locus_tag="PLN02_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52477.1"
                     /translation="MSSYQIAVLALIAGLAGQTIAGGLACERCLRGQGQTSDRFLWLV
                     LAGGALLLALAQGQALELAVHTGLHNLRQALLTGGGGLLYAIAVSGLSRRP"
     gene            complement(2434..>2624)
                     /locus_tag="PLN02_08880"
     CDS             complement(2434..>2624)
                     /locus_tag="PLN02_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HOI52478.1"
                     /translation="LAWLDVRELGRADPAAHFEAHGLGLSDGRAFGSPGWLRLNFGCP
                     ASLLDEALQRLAQACHAA"
BASE COUNT          383 a          808 c          981 g          452 t
ORIGIN      
        1 tcttctttgc cacccagcgg gcggtagcgg gcatcaactg catcaggccg gcggcgccga
       61 cgttcgagcg ggcgctggtg atgaaccgac tttcctggcg catcaggccg tacacccagg
      121 catcgtcgag cgcctgctgg cgagtgaccg ggcggatgct gtcggcgaac ggcgagagga
      181 agcgcaagct gtagtcgtgc tcgttgcggg tacggtcggc ggtgttgatc gcccggtccc
      241 agagttggtt gcggcgggcg agttcggcgg cggccagcaa ctgacgatca ttcatctcgc
      301 gcagggccca gttccattcg cggacggctt cggtgcgcat gtccaggcga aacaggctca
      361 aggcccgttg caggttggga ttgtcgcgag cagcggcaat ttcttcggct gacggcgggg
      421 tggcggatgc ctgggggcgc acctgacggc ccagttcctc gtcggccaga ttgccgtaga
      481 aatgcgcctg gccggcgatt ttcgcgaaca gggcgtcggc ttccgcggtg cggccgccgg
      541 atttgtaggc gcgtcccagc cagtagatcc actcgggtcg gttggcgagt tcgggcggca
      601 tggcctggat ggcgtcgcgg acctggcccc agttcagggc gcgcagcgcg gcgcggaccc
      661 gccactggag attttcgtcg gtgagcgcag catctccggc gcgagcgtac cagatgcttg
      721 cttctggcag gtgccggcgg gcgccgagca gcgcaatctg gctccaggcc cagccgccag
      781 cggagcgctc gagttgcgcc tcgcgcttgt cgagttgttc ggcggctacc agcgggtcgt
      841 tgaccgccac gtaacggatc gcgatcgccg ccagctcgcg gttgctccgc tgtcgctgcc
      901 agttggcccc ctggcgggcc aggtaggaca tcgatttctt tgtcgccgcg tcgaaatcgg
      961 cggtgccggg tacctggctg tcggggaggt aggcgagcgt ctgctgggcg gcgcccgggc
     1021 ggttgatttc gatctgccga cgggcgcgtt gccaggcggc atcgacgtcg atgcgaccgg
     1081 cagcgaccag ggcatcgagc aagggtttgc aggcctcgct gggttcaagc atgctcagcc
     1141 agtagccggc agcctcgtcg aggacgcggc gatcgccgcg ctgcaggcgg gcttgctgct
     1201 ggaggcaggt gcgttcggta tcgggggcga gcagtttggc gtattcgctt tcgacgcgtg
     1261 gccagtcgcc ctgtttggca agccaggtca gccagtcgct acgcaggcgt tcggcgacat
     1321 tgctgccctc gttgcgggcc aggaaggccg ggatgccgtc ggcgtcattg cgcgccagac
     1381 ggttgcgcag gagatagttt tcggcgtaaa tggccagcgg atggccgcgg gtttcgccga
     1441 cggcgctggc cagccgggcg gcatcgccct tgcggaaggc ttcgcgggcg gcgatgaagg
     1501 cgctgtcgtc ggcgctctgg gcaaaggcgg aaacgcccca gacaagcagg gcgatgatgg
     1561 gggaaaactt catgctggat tcgggtgatg acgggtcgtt ttgaggatag cacgctatgg
     1621 cgtcgtgatt tgcggcgcga catggtcgcc cgtcgggaga tgctgggcga gaaggagcgc
     1681 gcccgcctgt cggcggcgat cgtcggctgg ctgcggaagc atttcccgcc gccggccagt
     1741 tgtgccttct gctggccgat caagcacgaa ccggacgtcc gcgccatcat tgacgagtgg
     1801 accggtcgcg gtacgcgggc ggccttgccg gtggtggtgg ccgaggcgac gccgctgcag
     1861 tttcgcgaat ggcgggccgg cgaccgcctg caggccgatc gttacgggat tccgacgccg
     1921 gtcgatggcg actggctgca gcccgaactg atcctgctgc cgctcaacgg cttcgatgcc
     1981 gccggttacc gtctcggcta tggcgggggc tatttcgacc gcacgctggc ggcgctgacg
     2041 ccacggccgc tgacggtcgg cgtcggtttc gagatcaacc gtctcgatga catccgtccg
     2101 gaagtgcacg atcagcgtct ggactggatt gtcaccgagg ctggtgcgtt caaggtcgcc
     2161 ggctgaggcc gctcaccgcg atcgcgtaga gcagcccgcc gccgccggtg agcagcgcct
     2221 ggcgcagatt gtgcagtccg gtgtgcaccg ccagctccag ggcctggcct tgtgccaggg
     2281 caagcagcaa ggcgccgccg gcgagcacca gccagaggaa acggtcgctc gtctgcccct
     2341 ggccgcgcag gcagcgttcg caggcgaggc caccggcgat ggtctgcccg gcgaggccgg
     2401 caatcagtgc cagcacggca atctggtagc tgctcatgcg gcgtggcagg cttgcgccag
     2461 acgttgcaga gcctcgtcga gcagactcgc cgggcagccg aagttcaggc gtagccagcc
     2521 aggggatccg aaagcgcggc cgtcggaaag accgagacca tgggcctcga aatgcgcggc
     2581 cggatcggcg cggccgagtt cgcggacatc gagccaggcc aggt
//
LOCUS       DAOSDX010000382         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13754_length_2622_cov_3.343981, whole genome shotgun sequence.
ACCESSION   DAOSDX010000382 DAOSDX010000000
VERSION     DAOSDX010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13754_length_2622_cov_3.343981"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PLN02_08885"
     CDS             complement(<1..1444)
                     /locus_tag="PLN02_08885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOI52479.1"
                     /translation="MKPALQLKLSQHLALTPQLQQSIKLLQLSTVEMQQEIERYLLEN
                     PMLERDDDGPTESYAAAQQFDSPDKTSQTDNTAAPESREDVQGEPPMTPDDVDDDRWS
                     SDAGTFTGAGRDEDDDTDPQDSHAANVSLREHLGWQLGMTQMSERDRSLVRFLIEALD
                     DDGYLATPLEELWETLPAEYEVELEELDIALRHIQNFDPTGVGARDLRECLLLQLKVE
                     EDCAERTLAMAIADKHLELLAARDFARIRRLTGCDDEILKGAHELITSLNPRPAAGYS
                     QIEARYITPDVIVKKLKGKWTAYINPDAYPRLRINRLYAEILAKQRRGNGNLSGQLQE
                     ARWLIKNVQQRFETIHRVTQAIVDRQRQFFEHGEVAMRPLVLREIADLLGLHESTISR
                     VTSQKYMATPRGIFELKYFFGSHVSTDTGGACSATAIRALIKQLISAEDGKKPLSDSQ
                     LSEILGQQGIVVARRTVAKYRESLNIPPVNL"
     gene            complement(1472..2206)
                     /gene="lptB"
                     /locus_tag="PLN02_08890"
     CDS             complement(1472..2206)
                     /gene="lptB"
                     /locus_tag="PLN02_08890"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOI52480.1"
                     /translation="MSLPSATLSAHNLKKRYKARTVVEDVSFEVRSGEVVGLLGPNGA
                     GKTTCFYMVVGLVVADGGEVYIDEERLTHQAMHARARLGLSYLPQEASVFRKLNVEEN
                     IQAVLELQKLSPTEIAARLEELLSELHIGHVRHVNAAALSGGERRRVEIARALATNPR
                     FILLDEPFAGVDPIAVLEIQKIIRFLKERGIGVLITDHNVRETLGICDHAVIINAGHV
                     LASGRPEEIVDNESVRKVYLGEHFRL"
     gene            complement(2203..>2622)
                     /gene="recX"
                     /locus_tag="PLN02_08895"
     CDS             complement(2203..>2622)
                     /gene="recX"
                     /locus_tag="PLN02_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HOI52481.1"
                     /translation="NRALQLLARREHSRAELHARLATQAESGEALLAVLDALEQQGLL
                     SDDRYARQRVVARAGRYGNARLRQELKQRGIGESEIEAALPEAGDELERCRAIWARKF
                     GQPPETPEARAKQTRFLQYRGFSHEAIRHVLRGVEDE"
BASE COUNT          449 a          797 c          843 g          533 t
ORIGIN      
        1 gtaaattgac cggcgggatg ttcaacgatt cgcggtattt ggcgaccgtg cgtcgggcaa
       61 cgacgattcc ctgctggcct agtatttcgg ataattgact atccgacaag ggcttcttgc
      121 catcctcggc cgaaatcaat tgcttgatca gggcacggat ggcggtggca gaacaggcac
      181 cgccagtatc ggtggatacg tggctgccaa agaagtattt gagctcgaaa attccccgcg
      241 gcgtcgccat gtacttctgg ctcgtgacgc gtgaaatggt cgattcgtgg agaccgagca
      301 ggtcggcgat ctcgcgcagg accagcggcc gcatcgcgac ttcgccgtgc tcgaagaact
      361 ggcgctggcg gtcgacgatg gcctgggtga cgcggtggat ggtctcgaaa cgctgctgga
      421 cgttcttgat cagccagcgc gcttcctgca gctggccgct caggttgccg ttgccgcgcc
      481 gctgcttggc gaggatttcc gcgtacaaac ggttgatacg caggcgaggg taggcatcgg
      541 ggttgatgta ggccgtccac ttgcccttga gtttcttgac gatgacatcg ggcgtgatgt
      601 aacgggcttc aatctgcgaa tagccggcag ccggccgcgg attgaggctg gtgatcagct
      661 cgtgggcgcc cttgaggatt tcgtcgtcgc aaccggtgag ccggcggatc cgcgcaaagt
      721 cacgggcagc caggagttcc aggtgcttgt cggcgatggc catggccagc gtccgctcgg
      781 cgcaatcttc ctcgaccttg agctgcagca gcagacactc gcgcagatcg cgggcgccaa
      841 caccggtcgg atcgaagttc tggatgtggc gcagggcgat gtcgagttct tcgagttcga
      901 cttcgtactc ggcgggcagc gtttcccaga gttcctcgag cggcgtcgcc aggtaaccgt
      961 cgtcgtcgag cgcctcaatc aggaaacgga ccagactgcg atcgcgttcc gacatctggg
     1021 tcatccccag ttgccagccg agatgctcgc gcaagctgac attggccgcg tgactgtcct
     1081 gcgggtcggt atcgtcgtcc tcgtcgcgcc cggcgccggt aaaggtgccg gcatcgctcg
     1141 accagcggtc gtcgtcgacg tcgtcaggcg tcatcggcgg ctcgccctgg acgtcctcgc
     1201 ggctttccgg cgcagcagtg ttatccgtct ggctcgtctt gtccggtgaa tcgaattgct
     1261 gggcggccgc gtacgattcc gtcgggccgt cgtcgtcacg ctccagcatc ggattttcca
     1321 gcaggtagcg ttcgatctcc tgctgcatct cgaccgtcga cagttgcagc agcttgatcg
     1381 actgctgcag ttgcggcgtc agggcgagat gttgcgagag tttgagctgg agagccggtt
     1441 tcattgcgga aatggtctgg tcacgaagag gtcaaagccg gaaatgttca cccagatata
     1501 ccttgcgcac gctttcgttg tcgacaattt cttccggccg tccagaagcc aggacatggc
     1561 cggcattgat gatcaccgcg tgatcgcaga tgccgagggt ctcgcgaaca ttatggtcgg
     1621 taatcaggac gccgatgccg cgttccttga ggaaacggat gattttctgg atttccagca
     1681 cggcgatcgg gtcgacaccg gcaaagggtt cgtcgagcag aatgaagcgc gggttggtcg
     1741 ccagcgcccg ggcaatctcg acgcgccgcc gctcgccgcc ggaaagcgcc gccgcattga
     1801 cgtggcggac atggccgatg tgcagttcgc tcaacaactc ctcaagacgg gcagcgatct
     1861 cggtgggcga cagcttctgc agttcgagca ccgcctggat gttttcctcg acgttcaact
     1921 tgcggaacac cgacgcttcc tgtggcagat aggagagccc gagcctagcg cgggcatgca
     1981 tcgcctgatg cgtcaggcgc tcctcgtcga tatagacctc gccgccgtcg gcgaccacca
     2041 gcccgacgac catgtagaag caggtagtct tgccggcgcc gttcgggcca agcagaccga
     2101 ccacctcacc tgacctgact tcgaaggaaa catcctcgac gacggtccgc gccttgtagc
     2161 gcttcttgag gttatgggcg gacagggtgg ctgacggcag gctcattcgt cttcgactcc
     2221 acgcaggaca tggcggatcg cttcatggga aaagccgcga tactgcagga aacgcgtctg
     2281 ttttgcacgc gcttccggtg tttccggcgg ctgaccgaac ttcctggccc agattgcccg
     2341 gcagcgctcc agttcatcgc ccgcttccgg cagcgccgct tcgatctcgc tctcgccgat
     2401 accgcgctgc ttcagttcct gccgcagacg ggcattgccg tagcgcccgg cacgggcgac
     2461 cacgcgttgc ctggcatagc gatcgtcgga cagcagcccc tgctgctcca gggcatcgag
     2521 caccgccagc agcgcctcgc cggactcggc ctgggtggcc agtcgggcat gcagttcggc
     2581 ccggctgtgc tcccgccggg ccagaagttg cagggcgcga tt
//
LOCUS       DAOSDX010000383         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13775_length_2618_cov_2.843933, whole genome shotgun sequence.
ACCESSION   DAOSDX010000383 DAOSDX010000000
VERSION     DAOSDX010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13775_length_2618_cov_2.843933"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLN02_08900"
     CDS             <1..952
                     /locus_tag="PLN02_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOI52482.1"
                     /translation="CAVDNPVGLPPISVDEPTLTINFQVNTSPLAGTEGKFVTSRQIR
                     DRLHKELLTNMALRVHDTPNGDVFDVAGRGELHLGILLENMRREGYELAVGRPRVVKK
                     IIDGVECEPYEQLTVDVEEDTQGGVMESLGLRKGEMLDMVPDGRGRVRIEYRIPARGL
                     IGFQGEFMNLTRGNGLMSHVFDDYAPVKEGNVAERRNGVLISQDNGEAVAYALWKLQD
                     RGRMFVSPGEKLYEGMIIGIHSRENDLVVNPIKGKQLTNVRASGTDEAVRLVPPVQLS
                     LESAVEFIDEDELVELTPKSIRLRKRYLTEIERKRALRGL"
     gene            complement(1125..2141)
                     /gene="moaA"
                     /locus_tag="PLN02_08905"
     CDS             complement(1125..2141)
                     /gene="moaA"
                     /locus_tag="PLN02_08905"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOI52483.1"
                     /translation="MEPDIATTLHPPGTLVDRYGRHVTYLRLSITDRCDFRCTYCMAE
                     EMTFLPRSEVMSLEEALKVAKTFVGLGVSKLRLTGGEPLVRKNVMWLIERLGALPGLD
                     NLVMTTNGSQLETHASALKSAGVKRLNISLDSLNRERFRKITRIGDLDKVLRGIAAAT
                     ASGFRRTKLNTVMMRGVNDDEFIDLVRFAVDNGLDISFIEEMPLGDIQGRSQTHVSSE
                     ETRSRLGEHFSIVPSSENSGGPARYWRVADSETRVGFISPHSHNFCDSCNRVRVTAKG
                     ELYPCLGQNDALNLLPALRNSQDDSALRQAIIDSMGIKPFGHEFAEQMQNPKVVRFMS
                     MTGG"
     gene            complement(2309..2384)
                     /locus_tag="PLN02_08910"
     tRNA            complement(2309..2384)
                     /locus_tag="PLN02_08910"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2349..2351),aa:Asn,seq:gtt)
BASE COUNT          512 a          780 c          807 g          519 t
ORIGIN      
        1 gtgcgccgtg gacaacccgg tcggcctgcc gccgatcagc gtggacgagc cgacgctgac
       61 catcaatttc caggtgaata cctcgccgct cgccggcact gaaggcaagt tcgtcaccag
      121 ccgccagatc cgtgatcgcc tgcacaagga actgctgacc aacatggcgc tgcgcgtgca
      181 tgacaccccg aacggcgacg tcttcgacgt cgccggccgc ggcgaactgc acctcggcat
      241 cctgctcgag aacatgcgcc gcgaaggcta cgagctggcg gttggccgtc cgcgcgtggt
      301 caagaagatc atcgacggcg tcgaatgcga gccgtacgaa caactgaccg tcgacgtcga
      361 ggaagacacc cagggtggcg tcatggaaag tcttggcctg cgcaagggcg aaatgctcga
      421 catggtgccg gatggccgcg gtcgcgtccg tatcgagtac cgcatcccgg cccgcggcct
      481 gatcggcttc cagggtgaat tcatgaacct gacccgcggc aatggcctga tgagccacgt
      541 tttcgacgac tacgcgccgg tcaaggaggg taacgtcgcc gagcgtcgca atggcgtgct
      601 gatctcgcag gacaacggcg aagccgtcgc ctacgcgctg tggaagctgc aggatcgcgg
      661 ccgcatgttc gtcagcccgg gtgagaagct gtacgaaggc atgatcatcg gcatccacag
      721 ccgtgagaac gacctggtcg tcaacccgat caagggcaag caactgacca acgtccgcgc
      781 ttccggtacc gacgaagccg tgcgcctggt gccgccggtg cagctcagcc tggaatctgc
      841 cgtcgagttc atcgacgagg acgaactggt cgagctcacg ccgaagtcga tccgcctgcg
      901 caagcgctat ctgaccgaaa tcgagcgcaa gcgggcgttg cgcggcttgt aagataaagc
      961 gtatggcatt ttgccaaccc tgccgggagt tggtcgaacg tcacccgaag gcggcccggt
     1021 gggtcgcctt catccttggc atgtcgatct ttttcacctt cctgggccaa gtcagcgatc
     1081 cggtcaacgg gatcggtttc tgcaagattt acccgtaaag aacttcaacc gccggtcatc
     1141 gacatgaagc ggacaacctt cgggttctgc atctgctcgg caaattcgtg accgaaaggc
     1201 ttgatgccca tgctgtcgat gatggcctgg cgtaaagcgc tgtcgtcctg gctgttgcgc
     1261 agcgccggca gcaggttgag cgcatcgttc tggccgagac agggatagag ttctcccttg
     1321 gcggtgaccc gtacgcgatt gcagctgtcg cagaaattgt gactgtgcgg cgaaatgaag
     1381 ccgacccgtg tctcgctgtc ggcaacgcgc cagtaacgcg ccgggccgcc gctgttttcg
     1441 ctgctgggaa cgatactgaa atgctcgcca aggcgcgagc gggtttcctc ggacgaaaca
     1501 tgggtctgac tgcggccctg gatgtctccg agcggcattt cctcaatgaa tgaaatgtcg
     1561 agtccattat cgacagcgaa gcggacaaga tcgatgaatt catcgtcgtt gacgccgcgc
     1621 atcatcaccg tgttcagttt ggtgcgccga aatccgctcg ccgtcgcagc ggcgatgccg
     1681 cgcagcacct tgtccagatc gccgatccgg gtgattttcc ggaaacgttc ccggttcagg
     1741 ctgtcgaggc tgatgttcag gcgcttgacg ccggccgact tgagcgcact ggcatgggtt
     1801 tccagttgcg aaccgttggt cgtcatcacc aggttgtcga gtcccggcag cgcgccgagt
     1861 cgctcgatca gccacatcac gttcttccgc accaggggtt cgccgcccgt caggcgcaat
     1921 ttgctgacac cgagcccgac gaaggtcttg gcaactttca gcgcttcttc cagcgacatc
     1981 acctcgctgc gcggcaggaa agtcatttct tccgccatgc agtaagtgca gcggaaatcg
     2041 cagcgatcgg tgatcgacag gcgcaggtag gtcacatgtc gtccgtagcg atcgaccaga
     2101 gtgccgggtg gatgtagggt ggtggcgata tcgggttcca tgctgcgatt atcagtgcca
     2161 aaatcgctgt cgacaagcgc catggcgcgc gccggaagct gaaaaacccc ggcaatatcc
     2221 taggcttatg ctattatcgt ggccgttgtt cggggtaaga ccggccaaca aaaagcccag
     2281 caaagatgct gggcttttcg atgaagcttg gctccccgac ctgggctcga accagggacc
     2341 tacggattaa cagtccggcg ctctaccgac tgagctatcg gggaatcgat gaagccgaga
     2401 ttctatgagt ttctttaaaa cgcgtcaatg gattttgggc ggattttcgc gggtttttcg
     2461 ccggcgatcg tccgccccgt atccatcttt cgccatgttc cgcgcgcggc gcgatcaggc
     2521 gggcgcttgg tccagcttga aggcctggtg cagcacgcgc acggcgagtt ccagatactt
     2581 ctcgtcgacg acgacggaaa tcttgatttc cgaggtgg
//
LOCUS       DAOSDX010000384         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13854_length_2606_cov_2.918071, whole genome shotgun sequence.
ACCESSION   DAOSDX010000384 DAOSDX010000000
VERSION     DAOSDX010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13854_length_2606_cov_2.918071"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLN02_08915"
     CDS             <1..361
                     /locus_tag="PLN02_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52484.1"
                     /translation="APSETAAEPMNRVAVTPPSPVSRDAIFAAAREIARSEAQVDSHG
                     APRQAAIADRALLPGLAKALENKGAGTRVSRYSNGMVKVESAAGSTYCLLPPPAIPQV
                     GVPQVGVPQVSVAVTCP"
     gene            complement(526..2073)
                     /locus_tag="PLN02_08920"
     CDS             complement(526..2073)
                     /locus_tag="PLN02_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HOI52485.1"
                     /translation="MSRTTPSPLPEQPLSGGMLWFAAIILAAANFVAVLDMTIANVSV
                     PTIAGGLGATTSQGTWVITSYAVAEAITVPLTGWLAARFGAVRVFTIAMALFGGFSAL
                     CGLANSLGMLVFARICQGFAGGPLMPLSQTLLLRIFPKEKATAAVGIWSMTTLIAPVL
                     GPMLGGYICDEYSWPWIFYINLPIAFACAFIAWKLLKRYEEPLVRNPIDRIGLALLIV
                     WVGSLQLLLDEGKNLDWFASTEIVGLGIVAAIGFVTFLIWELHEEHPVVDLRVFRHRG
                     FSASVLTISLAFAAFFGANVLTPQWLQTYMGYTSTTAGMATAWTGIFAVFVAPIAAGL
                     SAKVDPRKLVFWGVLWLGIVCLWRTVATTDMGYWEVALPLMAMGLGLPFFFIPLTGLA
                     MASVDEPEMASAAGLMNFLRTLSGAFATSLITTLWDDQTTVNHAELVAVSDSDQTLQR
                     FFEGIGSAGETTLQNIDRLINAQAVMLATNQVMSIVAFSFIVAAFAIWLAPKPQRVID
                     PAASGGH"
     gene            complement(2083..>2606)
                     /locus_tag="PLN02_08925"
     CDS             complement(2083..>2606)
                     /locus_tag="PLN02_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOI52486.1"
                     /translation="SKEANAVLIAGVDEDSNQEIALARAKRDQAAIDLERTVIRSPID
                     GIVAKRQVQLGQRVQAGTTLLSVVPIQEMHVDANFKEGQLENVRIGQIATVTADIYGS
                     AVTYHGVVEGFSAGSGAAFAAIPAQNATGNWIKVVQRLPVRIRLNADELQANPLKAGL
                     SMAVRIDTGTLAN"
BASE COUNT          512 a          817 c          836 g          441 t
ORIGIN      
        1 cgccccgagc gaaactgcgg ccgaacccat gaaccgcgtc gcggtgaccc cgcccagccc
       61 ggtctcccgc gatgccattt tcgccgcggc gcgggaaatc gcccggagcg aagcgcaggt
      121 cgatagtcac ggtgcaccgc gacaggcagc aatcgccgat cgcgccttgc tgccaggctt
      181 ggcaaaggcg cttgagaaca agggcgcggg aacgcgtgtt tcccgctaca gcaatggcat
      241 ggtcaaggtc gagagcgcgg ccggaagcac ctactgcctg ctgcctccgc cggcgatccc
      301 gcaggtcggc gtgccgcagg tcggcgtgcc gcaggtcagc gtcgcggtca cctgtccgtg
      361 aaggttgcgg cggggtggcc gaatgtctac gtgccggatt gtcgtgtcgc tgtcacgaca
      421 attctgaacc aggcagagcc gcgaatagcc gactccgccg cccttctcga cgcggatctg
      481 cgccggattc ctttccttca tgtgcggccc ggctgcgatg gccagtcagt gtccgccgga
      541 cgcggcagga tcgatgacgc gctgcggctt gggcgccagc cagatggcga aggcggcgac
      601 gatgaagctg aaggcgacga tgctcatcac ctgattggtc gccagcatca ccgcttgcgc
      661 gttgatcagc cggtcgatgt tctgcagcgt ggtctcgccg gcgctgccga tgccttcgaa
      721 aaacctttgc agcgtctggt cggaatcgct gacggcgacc agctcggcgt gattgaccgt
      781 cgtctggtcg tcccacaggg tggtgatcag cgaggtggcg aaggcgcccg agagggtgcg
      841 caggaagttc atcaacccgg cggccgatgc catttccggt tcgtccacgc tggccatcgc
      901 cagcccggtc agcggaatga agaagaaagg caggcccagg cccatcgcca tcagtggcag
      961 cgcgacttcc cagtaaccca tgtccgtcgt cgcgaccgtg cgccagagac agacgatgcc
     1021 cagccagaga acgccccaga ataccagctt gcggggatcg actttggccg agagtcccgc
     1081 cgcaattggt gcgacgaaga ccgcaaaaat cccggtccag gctgtcgcca tccccgccgt
     1141 cgtcgaggtg tagcccatgt aggtctgcag ccattgcggc gtcagcacgt tggcgccaaa
     1201 aaacgcggcg aaagccagac tgatcgtcag cacgctggcc gagaagccac ggtggcggaa
     1261 cacacgtaaa tcgaccaccg ggtgttcttc atgcagctcc cagatcagga aagtgacaaa
     1321 gccgatggcg gcgacgatgc cgagcccgac aatttccgtg gaggcaaacc agtccaggtt
     1381 tttcccttcg tcgagcagca attgcagcga gccgacccag acgatcagca aggcgagtcc
     1441 gatgcggtcg atcggattgc ggaccagggg ttcttcatag cgcttgagca acttccaggc
     1501 aatgaaggcg caggcaaagg caatcggcag attgatgtag aaaatccacg gccaggaata
     1561 ctcgtcgcag atgtaacccc cgagcatcgg gccgagcacg ggcgcaatca gggtcgtcat
     1621 cgaccagatg ccgacggccg cagtggcttt ttcctttgga aatatccgca gcagcagcgt
     1681 ctgcgagagc ggcatcaacg ggccgccggc aaagccctgg caaatccggg caaagaccag
     1741 catgccgagc gagttggcca ggccgcagag ggcggaaaaa ccgccaaaca aggccatggc
     1801 gatggtaaag acgcgaaccg cgccgaagcg ggcggctagc cagccggtca gcggcacggt
     1861 gatcgcctcg gcgacggcgt aggaggtaat cacccaggtg ccctggctgg tcgtcgcacc
     1921 cagaccgccg gcgatggtcg gcacggaaac gttggcgatg gtcatgtcga gcacggccac
     1981 aaaattggcc gccgccagga tgatggccgc gaaccagagc atgccgccgc tcagcggctg
     2041 ctctggcaaa ggcgatgggg tcgtccggct catttcgttt cctcagttgg ccagggtgcc
     2101 ggtgtcgatc ctgaccgcca tcgacagtcc agccttcaag gggttggctt gcaattcatc
     2161 ggcattcaag cggatgcgaa ctggcaggcg ctgcaccacc ttgatccagt tgccggtggc
     2221 gttctgcgcc ggaattgcgg caaaggcggc accggatccg gcagaaaacc cttcgaccac
     2281 gccgtgatag gtcaccgcgc tgccataaat gtcggccgtg accgtggcga tctgtccgat
     2341 gcggacattt tccagttgtc cttccttgaa attcgcatcg acgtgcattt cctgtatcgg
     2401 cactaccgaa agcagcgtcg tacccgcctg gacacgctgc cccagttgca cctggcgctt
     2461 ggcgacgatg ccgtcgatcg gtgagcgaat gacggtgcgc tccagatcga tggctgcctg
     2521 atcgcgtttg gcgcgggcca gggcaatttc ctgattgctg tcttcatcga cgccggcaat
     2581 cagcaccgca ttggcctcct tcgagc
//
LOCUS       DAOSDX010000385         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13883_length_2602_cov_2.808009, whole genome shotgun sequence.
ACCESSION   DAOSDX010000385 DAOSDX010000000
VERSION     DAOSDX010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13883_length_2602_cov_2.808009"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="PLN02_08930"
     CDS             <1..1608
                     /locus_tag="PLN02_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOI52487.1"
                     /translation="RGESWPVTCAYSLHGVGEGGAGFVGQIPPLEAQAIYRRAGSQDF
                     QTLPLKRQTLWFFPDRDVGAVLFTGLLDVDDMLGDEIDHLLVALSEFGTPYPLNYLSV
                     VARRREDTSCNQIEGMRDEVLLPPASRGWAWEYLLLPEDHPRVAQPARAYARVRERMQ
                     HYLGMVAAAAEATDKAKEQDIAQVKAQTRALIRGEGLPETEADKMDWRRQLAAWPRDR
                     RRDDLIIRNADLSGLDFSGQSWQGSQLINVDLSACRFVDVDLRNMLFLGCKLDGASFE
                     NAVLDSCHFSECSLSKSRWKATQLATVQLIECDFSASTVLESQWKTTFFSESRGSKLS
                     FAKVAFDSVQMQKCAFPALSFADSTVDGHVFLDCQLPSLKISQSLWRKTSWVACKLPG
                     SSFENSRFATAVLADACDISGARWTQCRFEKVFLGAAKFDGGVCESCTWVEASMNALK
                     ASGSQWIACDLRQGNLMHADFSASRFQSSALRDANLYGADLRASEVHSSNLIGANLGY
                     VQREKNWQKKWKDNLMGRVIEVPRRQD"
     gene            1608..>2602
                     /locus_tag="PLN02_08935"
     CDS             1608..>2602
                     /locus_tag="PLN02_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOI52488.1"
                     /translation="MFDKLTGMPDFDTSAISGMPIKEKSFAGARFLAPPAGTHFVDCD
                     FSGTLWDGVTLDGQLFTRCNFSGAQFIGCILRTVRFFECVAASARFHRTLTTECHWES
                     GAWGDCQWQECRLARQMVNAVRGGNWEFRICDISFSTLSSLAFTGLRVESSLIRDLSL
                     VESKVHDQCWQECVMERVIAANSTFGGGQWLGCQGINNRWMGIKARHLAYQSCNFEQS
                     AWSTCEIEGGDFQSNLLRIAHFDSGQLQRLRFKGNTLFAIAFDGSLLRECEFRERDVS
                     HFSLRNARLESCDLNGITGSDLDARGCTMQGTTVSGARFVDANVAGQQPEDWRGA"
BASE COUNT          469 a          811 c          815 g          507 t
ORIGIN      
        1 cgtggggaaa gctggccggt gacttgcgcc tattcgctgc acggagtcgg tgagggtggc
       61 gccggctttg tcggtcagat tccgccgctg gaagcgcaag cgatctaccg ccgggccggc
      121 agccaggatt tccagaccct gccgctgaag cgccagaccc tgtggttttt cccggatcgc
      181 gacgtcggcg cggtgctatt taccggcctg ctcgacgtcg acgacatgct gggcgacgag
      241 atcgaccatc tattggtggc gctctcggaa ttcggcacgc cttacccgct gaattacctc
      301 tcggtcgtcg cccggcgccg cgaagatacg agctgcaacc agattgaagg catgcgcgat
      361 gaggtcctgc tgcccccggc cagccgcggc tgggcctggg aatacctgct attgcccgag
      421 gatcatccgc gcgtcgcgca accggcgcgg gcctatgcgc gggtccgcga acgcatgcag
      481 cattacctcg gcatggtcgc tgctgcggcc gaggcgaccg acaaggccaa ggaacaggat
      541 atcgcccagg tcaaggcaca gactcgcgcg ctgatccgcg gcgaaggcct gcccgagacg
      601 gaagccgaca aaatggactg gcgccggcag ctcgccgcct ggcctcggga cagacgtcgc
      661 gacgacctga tcatccgcaa cgccgatctc tccggcctgg atttttcggg gcagagctgg
      721 cagggctcgc agctgatcaa cgtcgatctc agcgcttgcc gtttcgtcga tgtcgacctg
      781 cgcaacatgc tcttcctcgg ctgcaaactc gatggcgcca gtttcgagaa cgccgtgctc
      841 gatagctgcc atttcagcga atgcagcctg agcaaatcgc gctggaaggc gacgcaactg
      901 gcgacggtgc agttgatcga atgcgatttc agcgccagca cggttctgga aagccagtgg
      961 aaaacgacct tcttcagcga atcgcggggc agcaagctga gcttcgccaa ggtggcgttc
     1021 gacagcgtgc agatgcagaa atgcgctttc cccgcgctgt ctttcgcgga tagcacggtc
     1081 gacggccatg tcttcctcga ttgccaattg ccatcgttga agatcagcca gtcgctgtgg
     1141 cgcaagacca gttgggttgc ctgcaagctg cccggcagca gcttcgagaa cagtcgcttt
     1201 gccaccgccg tcctcgccga cgcctgcgac atttccggtg cccgctggac gcagtgtcgg
     1261 ttcgagaagg tcttcctcgg cgccgccaag ttcgatggcg gcgtctgcga aagctgcacc
     1321 tgggtcgaag cgtcgatgaa tgcgctgaag gccagcggca gtcagtggat cgcctgcgat
     1381 ttgcgccagg gcaacctgat gcacgccgat ttctccgctt cccgtttcca gtccagcgcg
     1441 ctgcgcgacg ccaacctcta tggcgccgac ctgcgcgcca gcgaggttca ttccagcaac
     1501 ctgatcggcg ccaacctcgg ttacgtgcag cgcgagaaga actggcagaa aaagtggaag
     1561 gacaatctga tggggcgggt catcgaagtt cccaggaggc aggactgatg ttcgacaagc
     1621 tcaccgggat gcccgatttc gatacttccg cgatcagcgg catgccgatc aaggaaaaga
     1681 gttttgccgg agcgcgcttt cttgcgccgc ccgccgggac gcatttcgtc gattgcgact
     1741 tctccggaac cctctgggac ggtgtcacgc tcgatggcca attgttcacg cgctgcaatt
     1801 tcagcggcgc gcagttcatc ggctgcatcc tgagaaccgt ccgtttcttt gaatgtgtcg
     1861 ctgccagtgc ccgtttccac cggacgttga cgaccgaatg ccactgggaa agcggggcct
     1921 ggggcgactg tcagtggcag gaatgccggc tggctcgcca gatggtcaac gcggtgcgcg
     1981 gtggcaactg ggaattcagg atttgcgaca tttccttctc gaccctgtcg tcgctggcct
     2041 tcaccggctt gcgcgtcgaa agctcgctga tccgcgactt gtcgctggtc gagagcaagg
     2101 ttcatgacca gtgctggcag gaatgtgtga tggagcgcgt gatcgccgcc aattcgacgt
     2161 tcggcggcgg ccagtggctc ggttgccagg gcatcaacaa ccgctggatg gggatcaagg
     2221 cccgtcatct ggcctatcag tcctgcaatt tcgagcagtc cgcgtggtcc acctgcgaaa
     2281 tcgaaggcgg tgacttccag tcgaacctgc tgcgcatcgc ccatttcgat agcggtcagt
     2341 tgcagcgcct gcgtttcaag ggcaacaccc ttttcgccat tgccttcgac ggttctttgc
     2401 tgcgcgaatg cgaattccgc gaacgcgatg tctcccattt ctctttgcgc aacgcccgcc
     2461 tggaatcctg cgacctcaac ggcatcaccg gcagcgacct cgatgcgcgc ggttgcacga
     2521 tgcagggaac gacggtcagc ggcgcgcgct tcgtcgatgc caatgtcgcc ggccaacagc
     2581 cggaagactg gcgcggcgcc ga
//
LOCUS       DAOSDX010000386         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13893_length_2601_cov_2.994894, whole genome shotgun sequence.
ACCESSION   DAOSDX010000386 DAOSDX010000000
VERSION     DAOSDX010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13893_length_2601_cov_2.994894"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLN02_08940"
     CDS             complement(<1..979)
                     /locus_tag="PLN02_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52489.1"
                     /translation="MENFLRRGGRRTLTLIILLPLLLVGGSAMIFNLSTVYSLKQQQA
                     DDNAEQRELLALLNEATALSETLAAKQLKVSETLGLARDGKMAEAALYRSHASLVDQM
                     AALDRRVTDLAEHTRRLVIPEREAQALIEDFQGYRNYVVMASDIAAIDPGTASRHIDQ
                     ARAHFIAFAEHAHHLALVLGKRVEARNEAATSAFARHFASVRLTVIAGMLAMLALALL
                     IANYFSRRMSLLAEGMMSLAASRESPAPLPEIERLARRKHGEIAPLAAAVLDFRQALL
                     DRHDAEKQLRDYQQNLESLVEQRTAEVNQARLAAETANRAKSAFLANMSH"
     gene            complement(987..1610)
                     /locus_tag="PLN02_08945"
     CDS             complement(987..1610)
                     /locus_tag="PLN02_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOI52490.1"
                     /translation="MKTLTCLLSGILLSLSVAAADTPTFIEQPITRELFEQIRSGGFV
                     LYMRHGNTDNSRADRFPAVDLSDCSTQRLLSDAGRQLMRDIGRHLRSAKLPLNRVLVS
                     PMCRTRESAQLAIGEAFEISEPLMYSANMTSEQKQPRIAALKKLLAEPVKAGGNTLII
                     AHAPNLDDLIGFFVKPEGTIVVFRQTGNAGYEYAASIHPADWPGLLK"
     gene            complement(1720..>2601)
                     /locus_tag="PLN02_08950"
     CDS             complement(1720..>2601)
                     /locus_tag="PLN02_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HOI52491.1"
                     /translation="AAVRRLADYVIARHGGPRENSGQPYRDLLQAVAERQAALVARWL
                     GVGFIHGVMNTDNMTISGETIDYGPCAFLEAYDPGAVFSSIDHQGRYAYRRQGTIAQW
                     NLARFAETLLPLLDTDSERAVAVAMDIVEAFPDRHAAEWLRVFRAKLGIDEGGDAADD
                     RRLIEDFLALLQAGKADFTLAFRALYELATGSTPYLIAGEARFAAWLARWQARQPQRP
                     PALLAAMRTANPLYIARNHQVEAALDAAVDGADFAPFARLLDVVRNPFDARPDADAYA
                     TPAPAAFSDSYRTFCGT"
BASE COUNT          400 a          734 c          980 g          487 t
ORIGIN      
        1 cgtgcgacat gttggccagg aaggcgctct tcgcgcggtt ggcggtttcg gcagcgagcc
       61 gcgcctggtt gacttcggcg gtgcgctgtt cgaccaggct ttccagattt tgctggtagt
      121 cacgcagttg tttttcggcg tcgtggcggt cgagcagggc ctgacggaaa tcgaggacgg
      181 ctgccgccag cggcgcgatt tcgccgtgct tgcggcgtgc caggcgttcg atttcgggga
      241 gcggcgccgg gctttcgcgg ctcgcggcga ggctcatcat gccctcggcg agcagcgaca
      301 tccggcggct gaagtagttg gcgatcagca gcgccagcgc cagcatggcg agcatgccgg
      361 cgatgacggt caggcggacg ctggcgaagt gtctggcgaa ggcgctggtg gccgcctcgt
      421 tgcgtgcctc gacgcgtttg ccgagaacga gggcgaggtg gtgagcatgt tcggcaaagg
      481 cgatgaaatg ggcgcgcgcc tggtcgatgt ggcggctggc ggtgccggga tcgatggcgg
      541 cgatgtcgga ggccatcacc acgtaattgc ggtagccctg aaagtcctcg atcagggcct
      601 gcgcctcgcg ctccggaatc accaggcgcc gcgtgtgttc ggcgaggtcg gtgacgcggc
      661 ggtcgagcgc cgccatctgg tcgaccaggc tggcatggct gcggtagagc gccgcttcgg
      721 ccattttgcc gtcgcgggcg aggccgaggg tttcgctgac tttcagttgc ttggcggcga
      781 gcgtttcgct gagcgcggtg gcttcgttga gcagggccag cagttcgcgc tgttcggcgt
      841 tgtcgtcggc ctgctgctgc ttaaggctgt acaccgtgct caggttgaag atcatcgcgc
      901 tgccgccgac cagcaacagc ggcagcagga tgatcagggt cagggtgcgc cgaccgccgc
      961 gacggaggaa gttttccatc gccgcatcac ttgagcaggc cgggccagtc ggccgggtgg
     1021 atgctggcgg cgtattcgta accggcgttg ccggtttgcc ggaagacgac gatggtgcct
     1081 tctggtttga cgaagaagcc gatcagatcg tcgagattgg gggcgtgggc gatgatcagc
     1141 gtgttgccgc cggctttcac cggttcggca agcagctttt tcagggcggc gatccgcggc
     1201 tgcttttgct cactggtcat gttggccgaa tacatcagcg gttcgctgat ttcgaacgct
     1261 tcgccgatcg ccagttgggc cgattcccgg gtgcggcaca tcggactgac cagcacgcgg
     1321 ttgagtggca gtttcgcgct gcgcagatgg cggccgatgt cgcgcatcag ttggcggccg
     1381 gcgtcggaaa gcaggcgctg ggtcgagcag tcgctgagat ccaccgccgg aaaacggtcg
     1441 gcccggctgt tgtcggtatt gccgtgacgc atgtagagga cgaaaccgcc gctgcggatc
     1501 tgctcgaaga gttcgcgggt gatcggttgt tcgatgaagg tcggggtatc ggctgcggcg
     1561 acggacagcg aaagcaggat gccggacaac aggcaagtca aggtcttcat gaatttgcct
     1621 ccgcgggggt ttttgtaaag ttttgtacat taaagcacag caaatgctta tacgcgagcg
     1681 gtttatgcgt tcgtaattcc cctggccggg tcgggatttt caggtgccgc agaaagtccg
     1741 gtagctgtcg ctgaaggcgg cgggcgccgg ggtggcgtag gcgtcggcgt cggggcgggc
     1801 gtcgaacgga ttgcggacga cgtcgagcag gcgcgcgaaa ggcgcgaaat cggcgccgtc
     1861 cacggcggcg tcgagggcgg cctcgacctg atggttgcgc gcgatgtaca gcgggttggc
     1921 ggtccgcatc gccgccagta gcgctggcgg gcgctgcggc tggcgggcct gccagcgggc
     1981 gagccaggcg gcaaagcgcg cttcgccagc gatcaggtac ggcgtgctgc cggtggcaag
     2041 ctcgtaaagc gcacggaagg cgagggtgaa atcggccttg ccggcttgca gcagggcgag
     2101 gaaatcctcg atcaggcggc ggtcatcggc ggcgtcgccg ccttcatcga ttcccagctt
     2161 ggcgcggaag acgcgcagcc attcggcggc atggcggtcc gggaaagcct cgacgatgtc
     2221 catggcaacg gcgacggccc gctcggagtc ggtgtcgagc aggggcagca gggtttcggc
     2281 gaagcgggcg aggttccact gcgcgatcgt tccctgacga cggtaggcgt agcggccctg
     2341 gtggtcgatc gaactgaaca cggcgcctgg atcgtaggct tcgagaaagg cgcaggggcc
     2401 gtagtcgatc gtctcgccgg agatcgtcat gttgtcggtg ttcatcacgc catggatgaa
     2461 gccgacgccg agccagcgcg cgaccagcgc cgcctgcctt tcggcgaccg cctgcagcag
     2521 gtcgcggtag ggttggccgg agttttcccg gggcccgcca tggcgggcga ttacgtaatc
     2581 ggccaggcgc ctgactgccg c
//
LOCUS       DAOSDX010000387         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13894_length_2601_cov_2.957973, whole genome shotgun sequence.
ACCESSION   DAOSDX010000387 DAOSDX010000000
VERSION     DAOSDX010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13894_length_2601_cov_2.957973"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="PLN02_08955"
     CDS             complement(<1..1950)
                     /locus_tag="PLN02_08955"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HOI52492.1"
                     /translation="MTATPPSSRDERLRHWLAELEHILPAQAPIRDFVHHNTLHGFQH
                     LPFAEALAAAQAVTGARCYWPLGRFREAYASGRISRDDLLAALADAGIDDLEVPLCGG
                     LRRRDVWLAALLVDDTAPPAAARLTACRQLLPAPPAVDWREFAARRWAALAAGGGRQH
                     SLRGLLARLSGEDVLASVRGILQRHLAAHLDLGMAAWRNPAHGQGFYAAWKASAGRDL
                     AWELDELPDIRDEIQHLPDDAVFLLEAELRELQPDEAGWYDTLLRLALEMPGWSGMFL
                     RRDRQPLADAGVPVAMVDYLAVRVFLERRLCCELLRRLIGSPGGLAELDAYYRAHPAE
                     FLVRDAWHLGSLPEDLLAAAGRLCAVPPAAMLANAAAWEGLAPAVAAYFADAAAAAEA
                     RALAGLLEQLGEAAPSGLDESGAQALLDELARLTPELCGQLWLEAYEGHYRDQLFGAL
                     AANAGRRQAAPAPTAQVVMCMDDREEGTRRHLEEIAPDIETFGAAGFFGLPMYWQGLD
                     DAQPIALCPVVVTPERLVREQPLAGSEAAGARHARRRELRLRWRERLYQDTRRAPLAG
                     PLLTAAAGLPALAALTATTLAPAWLARATRRWRERFDGRVASEPVLTRAAADDPGRLP
                     VFSDAEQVERVEDFLRTIGLTDGFAP"
     gene            complement(1952..>2601)
                     /locus_tag="PLN02_08960"
     CDS             complement(1952..>2601)
                     /locus_tag="PLN02_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI52493.1"
                     /translation="AMQSWLLLLALGSLLYGALLAWRQQDLKGMVAYSSISHMGVVLL
                     GIAALNPAGFNGALLQMVAHGLAAALLFLIVGLLYRRTHRRDLADYGGLYGLAPRFAF
                     FIAFGLLAAIGLPGSAGFVAELHVLYGAYGRWGFAVVALGIAMLVAAAYGLRVIGRFC
                     QPAPAGAELADLTRSETVAASLLALAIVLLGVWPTPLLTLVGGSTARLAALFAGS"
BASE COUNT          401 a          947 c          951 g          302 t
ORIGIN      
        1 cggcgcgaaa ccgtcggtca ggccgatcgt gcgcaggaaa tcctcgaccc gctcgacctg
       61 ctcagcatcg ctgaacaccg gcagccgacc gggatcgtcg gcggccgcgc gggtcagcac
      121 cggttcgctg gcgacccggc cgtcgaatct ttcgcgccag cgccgggtcg cccgcgccag
      181 ccaggccggc gccagcgtcg tcgcggtcaa cgcggcgagc gccggcaagc cggcggcggc
      241 ggtcagcagg ggtccggcca gcggggcgcg ccgggtgtcc tggtagaggc gctcgcgcca
      301 gcgcaggcgc agttcgcgcc ggcgggcatg gcgggcgccg gcggcctcgc tgccagccag
      361 gggctgctcg cgcaccagcc gctcgggcgt caccaccacc gggcagaggg cgatcggctg
      421 cgcgtcgtcc agcccctgcc agtacatcgg caggccgaag aagccggcgg cgccgaaggt
      481 ttcaatgtcc ggcgcgatct cctcgaggtg gcggcgcgtg ccttcctcgc ggtcatccat
      541 gcacatcacc acctgcgccg tcggcgccgg cgcggcctgg cggcgaccgg cgttggcggc
      601 cagggcgccg aacagctggt cgcggtaatg cccctcgtaa gcctccagcc agagctggcc
      661 gcacaattcc ggcgtcagtc gcgccagttc gtcgagcagc gcctgcgctc cgctctcgtc
      721 gaggccgctc ggcgccgcct ctcccaattg ttcaagcagc ccggccaggg cacgcgcctc
      781 ggctgccgcc gcagcgtcgg caaaataggc ggcgaccgcc ggcgccagcc cttcccaggc
      841 cgccgcattg gccagcatag cggccggtgg cacagcgcac agccggccgg cggcggccag
      901 caggtcttcc ggcaggctgc cgaggtgcca ggcatcgcgg accaggaatt cggccggatg
      961 cgcccggtag taggcgtcga gttcggccag cccgcccgga ctgccgatca gccggcgcag
     1021 cagttcacag cacaggcggc gttcgaggaa aacgcggacg gccaggtaat cgaccatcgc
     1081 caccggcacc ccggcgtcgg ccagcggttg ccggtcgcgg cgcaggaaca tgccggacca
     1141 gccgggcatt tccagcgcca aacgcagcag ggtgtcgtac cagcccgcct cgtccggctg
     1201 caactcgcgc aactccgcct cgagcaggaa gacggcgtcg tccggcaggt gctggatctc
     1261 gtcgcggatg tcgggcagtt cgtcgagttc ccaggcgagg tcgcggccgg cgctggcttt
     1321 ccaggcggcg tagaagccct ggccgtgggc cgggttgcgc caggccgcca tgccgagatc
     1381 gagatgcgcc gccaggtggc gttgcaggat gccgcgcacc gaggccagca cgtcctcgcc
     1441 gctgagccgg gcgagcaggc cgcgcaagct gtgctggcgg ccgcccccgg cggccagcgc
     1501 ggcccagcgg cgggcggcga attcccgcca gtctacggcc ggcggcgccg gcagcagttg
     1561 ccggcaggcc gtcaggcggg cggcggcggg cggcgcggta tcgtcgacca ggagcgccgc
     1621 cagccagacg tcgcggcggc ggagcccgcc acatagcggt acttcgagat cgtcgatgcc
     1681 ggcatcggcc agcgcggcga gcaggtcgtc gcgactgatc cggccactgg cgtaggcttc
     1741 ccggaaacgc cccagcggcc agtagcagcg cgccccggtc accgcctgcg ccgccgccag
     1801 cgcctcggcg aacggcaggt gctggaagcc atgcagggtg ttgtggtgca cgaaatcgcg
     1861 gatcggcgcc tgcgccggca ggatgtgctc cagttcggcc agccagtgcc gcaggcgttc
     1921 gtcgcgggag gacggaggcg tcgccgtcat ctcagctccc cgcaaacagg gcggcaaggc
     1981 gtgccgtact gccgccgacc aaggtcagca gcggcgtcgg ccacaccccg aggaggacga
     2041 tcgccagcgc cagcaagctg gcggcaaccg tctcgctgcg cgtcaggtcg gccagttcgg
     2101 cacccgccgg cgccggctgg cagaaacggc cgatcacgcg caggccgtag gccgccgcga
     2161 ccagcatggc gataccgagc gccaccacgg caaatcccca gcgcccgtag gcgccgtaca
     2221 ggacgtgcag ttcggcgacg aaaccggcac tgcccggcag accgatcgcc gccagcaggc
     2281 cgaaagcgat gaagaaggcg aagcgtggcg ccagtccgta gaggccgccg tagtcggcga
     2341 ggtcgcggcg atgcgtgcgc cggtagagga gaccgacgat caggaacagc agcgccgccg
     2401 ccaacccgtg cgcgaccatc tgcagcagcg cgccgttgaa gccggcggga ttgagcgcgg
     2461 cgatgccgag caggacgacg cccatgtgcg aaatcgacga gtaggcgacc atccccttca
     2521 ggtcctgctg gcgccaggcg agcagcgcgc cgtagagcag gctgcccagc gccagcagca
     2581 gcagccagga ctgcatcgcc t
//
LOCUS       DAOSDX010000388         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13923_length_2597_cov_2.256884, whole genome shotgun sequence.
ACCESSION   DAOSDX010000388 DAOSDX010000000
VERSION     DAOSDX010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13923_length_2597_cov_2.256884"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLN02_08965"
     CDS             complement(<1..295)
                     /locus_tag="PLN02_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOI52494.1"
                     /translation="MHTAATVSPLSLGQRLAAFGHLCKPRVNSLIVFTAMIGMCLATP
                     DFPPLLRFIVAALGIALVSFAAAAMNCLVERTVDAKMARTSWRATARGDISPLE"
     gene            complement(306..860)
                     /locus_tag="PLN02_08970"
     CDS             complement(306..860)
                     /locus_tag="PLN02_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52495.1"
                     /translation="MNSVALPSPPDQRQGRLTLALIVGLLALPFVIAAALYFGGWHPS
                     RMTHHGHLLNPPRPLPAEILRSTGKNAEKWQLVLHVRGPCAADCRRRLDEMRRVHVAL
                     YKNMGRLGRALLTGHADDPALPGVRASQPDLLLLSAPPDTLADIADPLLLVDPQGRII
                     MTYPPDASPQGLRADLDRLLKYAG"
     gene            complement(857..1624)
                     /locus_tag="PLN02_08975"
     CDS             complement(857..1624)
                     /locus_tag="PLN02_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOI52496.1"
                     /translation="MDDASGSVATAQYARRPSAPARASRKQVAILGGLLLALLLPSFV
                     SLGLWQWRKAESKTALQIELDSRSHDAAIAMPSTPADVDALRHRRVILRGSYDAAHQV
                     LIDNRPYREQAGYHVITPLRLSGATMHVLVNRGWLPAPADHRQQPVAEVPAGEIEVSG
                     IAVLPAQRFFNLAAQPTSGWAPVWQNLDLERFRSAVPYPLQGLVVQLDPTAAGGYVRD
                     WPRPDERADRHRSYALQWFGFAIASLGIWGYFLIRRP"
     gene            1623..1823
                     /locus_tag="PLN02_08980"
     CDS             1623..1823
                     /locus_tag="PLN02_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HOI52497.1"
                     /translation="MLRIVVVLLLLGIVGSLFSGLFLLYRDRGQGERTVRALTLRIGL
                     SIALFLLLLGSFRFGFIPGYTQ"
     gene            complement(1811..>2597)
                     /locus_tag="PLN02_08985"
     CDS             complement(1811..>2597)
                     /locus_tag="PLN02_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOI52498.1"
                     /translation="MFLLALGFVQTMNDAGPGQWVMLAGAAVIVYVLFGWFGHVIAES
                     QSGAYRDWEDRSFRYGMIWFIATEVMFFAAFFGALFYMRVISVPRLGEMMAVHGVTLW
                     PDFAGTWPTSGPQGAPFTPMGAWGIPALNTALLLSSGATVTWAHWALLRDRRRQLVAG
                     LAATVLLGAIFLGFQAYEYHHAYTELGLTMGAGAYGATFFMLTGFHGFHVTLGTVMLA
                     VILLRSLAGHFSAERHFAFEAVAWYWHFVDVVWLILFVFVYWV"
BASE COUNT          437 a          808 c          968 g          384 t
ORIGIN      
        1 tttccagcgg cgagatgtcg ccacgggcgg tggcgcgcca actggtgcgc gccattttgg
       61 catcgaccgt gcgctcgacc aggcagttca tcgcggcggc ggcgaaggag accagcgcaa
      121 tgcccagcgc ggcgacgatg aaacgcagca gcggcgggaa atccggcgtc gccaagcaca
      181 tcccgatcat cgcggtgaac acgatcaggc tgttgacccg cggtttgcac aggtggccga
      241 aggcggccag acgttggccg agactcaggg gggagacggt ggcggcggtg tgcatggcgg
      301 ttctcctagc cggcgtattt gagcagacgg tcgaggtcgg cgcgcagtcc ttgcgggctg
      361 gcgtcgggcg gataggtcat gatgatccgg ccttgcggat cgaccagcag cagcggatcg
      421 gcgatgtcgg ccagggtgtc cggcggcgcg ctgagcagca gcaggtcggg ttggctggcg
      481 cgcacgccgg gcagcgcggg atcgtcggca tgcccggtga gcagcgcacg gcccaggcgg
      541 cccatgttct tgtagagggc gacgtggacg cggcgcatct cgtcgaggcg gcgccggcaa
      601 tcggcggcgc aggggccgcg gacgtgcagc accagttgcc atttttcggc gtttttcccg
      661 gttgaccgca ggatttctgc cggcagcggg cggggcgggt tgagcaggtg gccgtggtgc
      721 gtcatccgcg acgggtgcca gccgccgaag taaagcgcgg cggcgatgac gaagggcagg
      781 gcgagcaggc cgacgatcag ggccagggtc aggcggcctt ggcgctggtc gggcggggag
      841 gggagggcga cgctgttcat ggtcggcgga tcaggaaata gccccagatg ccgaggctgg
      901 cgatggcaaa accgaaccat tgcaaggcgt agctgcggtg gcggtcggcg cgttcgtccg
      961 ggcgcggcca gtcgcgcacg tagccgcccg ccgcagtcgg gtcgagctgg acgaccaggc
     1021 cttgcagcgg atagggcacg gccgagcgga agcgttcgag gtcgaggttc tgccagaccg
     1081 gcgcccaacc gctggtcggc tgcgccgcca ggttgaagaa gcgctgcgcg ggcagcacgg
     1141 cgatgccgct cacttcgatt tcgccggccg gcacctcggc cacgggctgc tggcgatgat
     1201 cggccggcgc cggcagccaa ccgcgattga ccagcacatg catggtcgcc ccggaaagcc
     1261 gcaagggggt gatgacgtga tagccggctt gctcccgata cgggcggttg tcgatcagca
     1321 cctggtgggc ggcgtcgtaa ctgccgcgca ggatgacccg gcgatggcgc agggcgtcga
     1381 cgtcggcagg cgtcgacggc atggcgatgg cggcgtcgtg gctgcggctg tcgagttcga
     1441 tctgcagcgc cgtcttgctt tcggccttgc gccattgcca caggccgagg gaaacgaagg
     1501 acggcagcag cagcgccagc agcagcccgc cgaggatcgc cacctgcttg cggctggccc
     1561 ttgccggcgc gctcgggcgg cgcgcatact gggccgtggc aactgacccg gaggcgtcgt
     1621 ccatgctcag gattgtggtg gttttgctgt tgcttggcat cgtcggcagc ctgttctccg
     1681 ggctcttcct gctctatcgc gaccgcggcc agggcgaacg cacggtacgc gcgctgaccc
     1741 tgcgcatcgg cctgtccatc gcgctcttcc tgttgctgct cggcagtttc cggtttggct
     1801 tcattcccgg ttacacccaa taaacgaaga caaaaaggat cagccagacc acgtcgacaa
     1861 agtgccagta ccaggccacc gcctcgaagg cgaagtggcg ctcggcgctg aagtggccgg
     1921 ccaggctgcg cagcaggatc accgccagca tgacggtgcc gagcgtgacg tggaaaccgt
     1981 ggaagccggt gagcatgaag aaggtcgcgc cgtaggcgcc ggcgcccatg gtcagtccca
     2041 gttcggtgta ggcgtggtgg tattcgtaag cctggaagcc gaggaagatg gcgccgagca
     2101 ggacggtcgc cgccagcccg gcgaccagtt gccggcgccg gtcgcgcaac agggcccaat
     2161 gcgcccaggt gacggtggcg ccggaactga gcagcagcgc ggtgttcaag gccgggatgc
     2221 cccaggcgcc catcggcgtg aacggcgcgc cctgcggacc gctggtcggc caggtgccgg
     2281 cgaagtcggg ccacagggtg acgccatgca ccgccatcat ctcgcccagg cgcggcaccg
     2341 agatcacccg catgtagaac agggcgccga agaacgcggc aaagaacatc acctcggtgg
     2401 cgatgaacca gatcatgccg tagcggaagg agcggtcttc ccagtcgcgg taggcgccac
     2461 tctgcgattc ggcgatgacg tggccgaacc agccgaacaa cacatagacg atgaccgccg
     2521 cgccggccag catgacccac tggccggggc cggcatcgtt catcgtctgc acgaagccga
     2581 gcgccagcag gaacatg
//
LOCUS       DAOSDX010000389         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13935_length_2594_cov_3.234344, whole genome shotgun sequence.
ACCESSION   DAOSDX010000389 DAOSDX010000000
VERSION     DAOSDX010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13935_length_2594_cov_3.234344"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PLN02_08990"
     CDS             <1..1441
                     /locus_tag="PLN02_08990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOI52499.1"
                     /translation="DVDTLHARNHPAWRRVKFDEVLAQQLSLRRAYLARREQGAPVLV
                     AQDDLGARLLDRLPFGLTGAQLRAMAEIAGDLAQPYPMQRLLQGDVGAGKTIVAALAA
                     CQAISAGWQAAFMAPTEILAEQHYLKLRDWLEPLGVRVAWLSGSLKSKAKRAQLAATA
                     ADAQLVVGTHALIQDGVDFARLGLAIVDEQHRFGVAQRLALRKKGSNPHQLMMSATPI
                     PRTLAMSYYADLDVTVLDELPPGRTPIKTRLVADNRRQDVVGFVKKHVEEGRQAYWVC
                     PLIEESEALQLQTAEETYAQLSADLPGLSVGLVHGRLKADEKQAVMSAFAAGDIDVLV
                     ATTVIEVGVDVPNASLMVIEHAERFGLSQLHQLRGRVGRGQYESSCVLIYAGPLGEIA
                     RQRLKIIFENTDGFEIARQDLQLRGPGEFVGARQSGVPLLRYADLELDADLVEMARQI
                     AEEMLTEHPALAEKHLQRWLGAREELLKS"
     gene            complement(1431..2321)
                     /locus_tag="PLN02_08995"
     CDS             complement(1431..2321)
                     /locus_tag="PLN02_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOI52500.1"
                     /translation="MQPSRSEFFDVNGVRLHIRRWGRTDAPTLFLLHGWMDVSASWQF
                     VVDALAREWNIVAPDWRGFGQSQWLNRPYFFAEHIGDLAAIIDRYAPDEAVRIAGHSM
                     GGILGTLYAGIRPQRVQRLVSLEGIGIAPTTGDMAPARYRQWLEQLEEPPRMHAYADR
                     QAFARRLLKSDPYLTEERAEFLSRHLARIGDGTNRQGEARHGVIWNGDPWHKATSPYL
                     FRLEESMAIWREVACPVLWISGRQSWVVRDFAARPGDWEARRACFADIRESWIDDADH
                     MLHHDQPAQVAALIEDWFRT"
BASE COUNT          434 a          855 c          883 g          422 t
ORIGIN      
        1 cgacgtcgac accttgcacg cgcgcaatca tccggcctgg cggcgggtca aattcgacga
       61 ggtgctggcc cagcaactgt cgctacgccg tgcctacctc gcccgccgcg aacagggcgc
      121 gccggtgctg gtggcgcagg acgacctcgg cgcccgcctg ctcgaccgcc tgcccttcgg
      181 tctgaccggc gcgcagttgc gggcgatggc cgagattgcc ggcgacctcg cccagcctta
      241 cccgatgcag cgcctgctgc agggcgatgt cggcgccggc aagaccatcg tcgccgcgct
      301 ggcggcctgc caggcgattt ccgccggttg gcaggcggcg ttcatggcgc cgaccgaaat
      361 tctcgccgaa caacattatc tgaagctgcg cgactggctg gaaccgctcg gcgtgcgcgt
      421 cgcctggctg tccggcagcc tcaaatcgaa agccaagcgc gcgcaactgg cggcgacggc
      481 ggccgacgcg caactggtgg tcggcaccca tgcgttgatc caggatggcg tcgacttcgc
      541 ccggctgggc ctggccatcg tcgacgagca gcaccgcttc ggcgtcgccc agcgcctggc
      601 gctgcgcaag aagggcagca acccgcacca actgatgatg tcggcgacgc cgatcccgcg
      661 cacgctggcg atgagctact acgccgacct cgacgtcacc gtcctcgacg aactgccgcc
      721 cggccggacg ccgatcaaga cccggctggt tgccgataat cggcgccagg acgtcgtcgg
      781 tttcgtgaaa aaacatgtcg aggagggccg ccaggcctac tgggtgtgcc cgctgatcga
      841 ggagtcggaa gccctgcaat tgcagacggc ggaggaaacc tatgcgcaac tttcggccga
      901 tttgccgggc ctgagcgttg gcctggtaca cggccgcctc aaggccgacg agaagcaggc
      961 ggtgatgagc gccttcgccg ccggcgacat cgacgtgctg gtggcgacga cggtgatcga
     1021 agtcggcgtc gatgtgccca acgccagcct gatggtcatc gagcacgccg aacgtttcgg
     1081 cctctcgcaa ctgcaccagt tgcgcggccg ggtcggccgt ggccagtatg aatcgagctg
     1141 cgtgctgatt tacgccgggc cgctcggcga gattgcccgc cagcgcttga agatcatctt
     1201 cgagaatacc gacggcttcg agatcgcccg ccaggacctg caattgcgcg gccccggcga
     1261 attcgtcggc gcccgccaga gcggcgtgcc gctgctgcgc tacgccgacc tggaactgga
     1321 cgccgacctg gtcgagatgg cgcgccagat tgccgaggaa atgctcaccg agcacccggc
     1381 gctggccgag aaacacctgc agcgctggct cggcgcccgc gaggaactgc tcaagtcctg
     1441 aaccagtcct cgatcagcgc tgcgacctgt gccggctgat cgtggtgcaa catgtggtcg
     1501 gcgtcgtcga tccagctttc gcggatgtcg gcgaagcagg cgcggcgcgc ttcccagtcg
     1561 cccgggcggg cggcgaaatc gcgcacgacc caggactggc ggccgctgat ccagagcacc
     1621 gggcaggcga cttcacgcca gatcgccatc gattcttcga ggcggaacag gtagggcgag
     1681 gtcgccttgt gccaggggtc gccgttccag atcacgccat gccgggcttc gccctggcgg
     1741 ttcgtgccgt cgccgatgcg cgccaggtgg cgggaaagaa actcggcgcg ctcctccgtc
     1801 aggtagggat cgcttttcag cagccggcgg gcgaaggcct ggcggtcggc ataggcgtgc
     1861 atgcgcggcg gttcttccag ttgctccagc cactgccggt agcgggccgg cgccatgtcg
     1921 ccggtggtcg gcgcgatgcc gatgccctcc agcgacacca ggcgctgcac gcgttgcggc
     1981 cggatgccgg cgtacagcgt gccgaggatg ccacccatgc tgtggccggc gatgcgtact
     2041 gcctcatccg gggcgtagcg gtcgatgatc gccgccaggt cgcctatgtg ctcggcgaag
     2101 aaatacggcc ggttcagcca ctgcgactgg ccgaagccgc gccagtcggg ggcgacgatg
     2161 ttccactccc gcgccagggc gtcgacgacg aactgccagg aggcggaaac gtccatccag
     2221 ccgtgcagca ggaacagcgt cggggcgtcg gtgcggcccc agcggcggat gtgcaggcgg
     2281 acgccgttga cgtcgaagaa ttcggatcgg gaaggctgca tggtcgctga aatcaaggag
     2341 tcgggctggc gattctagcc gatgcggcaa atgcgtcgcc gggtgctgtt ccgatattgc
     2401 caagggcata taatgttggc atatcaaacc aagcaaaagt cagggagcct tcatgaaacg
     2461 tcgcgatttt gtcctgctcg ccaccatgtt ttccggcgtc agcttcgtca atcaagccag
     2521 tgcgcagttc aactgggggc gggcgctcga tgccggtcgc aatctcctgc agtcggaaac
     2581 catttccgat tccg
//
LOCUS       DAOSDX010000390         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13936_length_2594_cov_2.600236, whole genome shotgun sequence.
ACCESSION   DAOSDX010000390 DAOSDX010000000
VERSION     DAOSDX010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13936_length_2594_cov_2.600236"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLN02_09000"
     CDS             complement(<1..1092)
                     /locus_tag="PLN02_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOI52501.1"
                     /translation="MKGQLRNDATAKAWLNGLAVRGRPWRELAILAGVLQGVGVIVMA
                     ALLAALIHGLVMDGKSLAELAAYWYGLPFAFAWRALAGWAREEAGARAAAKIRAALRR
                     GLLDQVQALGPAWVAGQTGGGLVSTLLEQVDGLDGYFARYRPQMFIAVLIPLLILGVV
                     FPQNWAAGLIFLLTAPLIPVFMILVGLGTQARQTRQLQMLTRMSGHFLDLVRGLGNLR
                     LLDAHRAQTPRVAAVAEEFRVRTMSVLRMAFLSSTVLEFFASVSIAMLAVYLGFSLLG
                     HLDFGYYEAPPDLALSFFILLLAPEFYLPLRELGTHYHARAEALAAAEQLQPIFAASS
                     PQCRGGSQQPADKAPAIRLQRLSFAHQPGV"
     gene            complement(1234..>2594)
                     /gene="groL"
                     /locus_tag="PLN02_09005"
     CDS             complement(1234..>2594)
                     /gene="groL"
                     /locus_tag="PLN02_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764708.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOI52502.1"
                     /translation="AIVREGMKYVAAGMNPMDLKRGIDKAVAATIEELKQISKPCTTT
                     KEIAQVGSISANSDSDIGEIIANAMEKVGKEGVITVEDGKSLANELDVVEGMQFDRGY
                     LSPYFINNQDKQIALLDNPFVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVDGE
                     ALATLVVNNIRGILKTVAVKAPGFGDRRKAMLEDIAILTGGTVISEETGLSLEKATLK
                     DLGQAKRVEIAKENTTIIDGAGDAASIEARVKQIRVQIEEATSDYDREKLQERVAKLA
                     GGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALLRARAAVKSLKG
                     ENHDQDAGIKLILKAIEAPLREIVANAGDEPSVVVDKVVRGKGNYGYNAATGEYGDMV
                     EMGVLDPTKVTRTALQNAASVAGLMLTTECMIAELAEDKPAGGMPDMGGMGGMGGMGM
                     GM"
BASE COUNT          436 a          838 c          852 g          468 t
ORIGIN      
        1 cacgccgggc tgatgtgcga agctcagacg ctgcagacgg attgccggcg ccttgtccgc
       61 cggctgctga ctgccgccgc ggcattgcgg cgaactggca gcgaagatcg gctgcaactg
      121 ttcggcggcg gccagcgcct cggcgcgcgc gtggtagtgc gtccccagtt cgcgcaacgg
      181 caggtagaac tctggcgcca gcagcaggat gaagaaggaa agcgccaggt cgggcggcgc
      241 ctcgtagtaa ccgaaatcaa gatggccgag cagcgagaac cccaggtaga cagccagcat
      301 ggcgatcgac accgaggcga agaattcgag cacggtcgac gacaggaaag ccatgcgcag
      361 cacgctcatc gtgcgcaccc ggaactcctc ggcgacggcg gcgacgcgcg gcgtctgcgc
      421 gcggtgcgcg tcgagcaggc gcaggttgcc cagaccgcgc accaaatcga gaaaatgtcc
      481 gctcatccgg gtcagcatct gcagttgccg ggtctggcga gcctgcgtgc cgaggccgac
      541 gaggatcatg aacaccggga tcagcggcgc cgtcagcagg aagatcaggc cggcggccca
      601 gttctggggg aagacgacgc ccaggataag caagggaatg aggacggcga tgaacatctg
      661 cggccggtaa cgggcgaaat aaccgtccag cccatcgacc tgttcgagca gcgtggacac
      721 caggccgccg ccggtctgcc cggccaccca ggccgggccg agcgcctgta cctggtcgag
      781 caggccgcgg cgcaacgccg cccggatctt cgccgccgcg cgcgcccccg cctcttcccg
      841 cgcccagccg gcgagcgccc gccaagcgaa cgcaaacggc aggccgtacc agtacgccgc
      901 caattcggcg agcgacttgc catccatcac caggccatgg atcagcgcgg ccagcaaggc
      961 cgccatgacg atgacgccga cgccctgcag cacaccggcg aggatcgcca gttcgcgcca
     1021 gggccgtccc ctgaccgcca gtccgttgag ccaggccttt gccgtggcgt cgttgcgcag
     1081 ttgtcctttc accttcccgc cgtcctcttg tcccggactc gcctggcggc gcgtccgcat
     1141 tggtggcggc accccctgac gggagcacca aacaaaacac ccggccgcag ccgggtgtct
     1201 gtgcttgctt gagcgatgct caggcggaca ttattacatg cccataccca tgccgcccat
     1261 accgcccatg ccgcccatgt caggcatgcc gccggccggc ttgtcttcgg ccagttcagc
     1321 gatcatgcat tcggtggtga gcatcaggcc ggccacggaa gcggcgttct gcagcgcggt
     1381 gcgggtgacc ttggtcgggt ccagcacgcc catttcgacc atgtcgccgt attcgccggt
     1441 agcggcgttg taaccgtagt tacccttgcc acggaccacc ttgtcgacca ccacggacgg
     1501 ctcgtcgccg gcattggcaa cgatttcgcg cagcggagct tcgatcgcct tgaggatcag
     1561 cttgatgccg gcgtcctggt cgtggttttc gcccttcagc gacttgaccg cggcacgggc
     1621 gcgcagcagg gcaacgccgc cgccaggaac gatgccttct tcaacggcgg cacgggtggc
     1681 gtgcagcgcg tcttcgacac gggccttctt ttccttcatt tcgacttcgg tggcagcacc
     1741 gaccttgatc acggcaacac cgccggccag cttggcgacg cgctcttgca gcttttcacg
     1801 gtcgtagtcg gaagtggctt cctcgatctg cacgcggatt tgcttgacgc gggcttcgat
     1861 cgaagcggca tcgccggcgc cgtcgatgat ggtggtgttt tccttggcga tttcaacgcg
     1921 cttggcctgg cccagatcct tcagggtggc cttttccagg gacagaccgg tctcttcgga
     1981 gatgacggta ccgccggtca ggatggcgat gtcttcgagc atggccttgc gacggtcgcc
     2041 gaagccagga gccttgacgg caacggtctt caggatgccg cggatgttgt tcacgaccag
     2101 ggtggccagc gcttcgccat cgacgtcctc ggcgatgatc agcagcgggc gggaggcctt
     2161 ggcgacttgt tccagcaccg gcagcaggtc gcggatgttg gagatcttct tgtcgaagag
     2221 caggacgaac gggttgtcga gcagggcgat ctgcttgtcc tggttgttga tgaagtaggg
     2281 cgacaggtag ccgcggtcga actgcatgcc ttcgacgacg tcgagttcgt tggccaggga
     2341 cttgccgtct tcaacggtga tgacgccttc cttgccgacc ttttccatgg cattggcgat
     2401 gatttcgccg atgtcggaat cggagttggc ggagatcgaa ccgacctggg cgatttcctt
     2461 ggtcgtcgtg cacggcttgg agatctgctt cagttcttcg atggtggcgg cgacggcctt
     2521 gtcgatgccg cgcttcagat ccatcgggtt catgccggcg gcgacgtact tcatgccttc
     2581 gcggacgatg gcct
//
LOCUS       DAOSDX010000391         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_13948_length_2592_cov_3.960978, whole genome shotgun sequence.
ACCESSION   DAOSDX010000391 DAOSDX010000000
VERSION     DAOSDX010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13948_length_2592_cov_3.960978"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLN02_09010"
     CDS             complement(<1..734)
                     /locus_tag="PLN02_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOI52503.1"
                     /translation="MLSQLFKFHGGVKPASHKDLSNQAPIVTAPLPSRLIVPLHQSIG
                     GTPRPVVAVGDRVLKGQLIGEADGWLSAAIHAPTSGTVVEIAMHTRPHPSGLGSLCVV
                     IEPDGRDEWIAHGPADYAAMTPADICSYLQRYGIVGLGGAGFPTHGKLTANPKVAMAE
                     LIINAAECEPYITCDDRLMRERAEEIVRGTAIFRDMLKPQQVIIGVEDNKPEAIAALR
                     AAVAALAEPFAVVPVPALYPAGGAKQ"
     gene            complement(734..1306)
                     /gene="rsxB"
                     /locus_tag="PLN02_09015"
     CDS             complement(734..1306)
                     /gene="rsxB"
                     /locus_tag="PLN02_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HOI52504.1"
                     /translation="MLLAIAIMALGALVLGAALGFSSIKFKVEGDPLVEKIDAILPQT
                     QCGQCGYPGCKPYAEAIAKGEADINLCPPGGMEGVQRLADLLGREVKALEAEEKPKQV
                     ARIDENTCIGCTLCIQACPVDAIVGAAKQLHTILTQQCTGCELCLPPCPVECIHMEVV
                     PETLDNWKWKYPVVSIHPASPAASPEKEAA"
     gene            complement(1312..1896)
                     /gene="rsxA"
                     /locus_tag="PLN02_09020"
     CDS             complement(1312..1896)
                     /gene="rsxA"
                     /locus_tag="PLN02_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOI52505.1"
                     /translation="MSHYIFLLVGAVLVNNVVLVKILGLCPFMGVSKKLETAYGMGAA
                     TTFVLTLATGSSYIIDHFLLMPFGLEYLRTLSFIVTIAAIVQLTEMVIAKTSPTLQQT
                     LGIYLPLITTNCAVLGVPLLNVANGYDFVESLLYGAGSAVGFSGVLILFAGIRERIEG
                     ADVPTHFRGTAIALVTAGLMALAFMGFAGLDKYQ"
     gene            complement(1973..>2592)
                     /gene="pyrD"
                     /locus_tag="PLN02_09025"
     CDS             complement(1973..>2592)
                     /gene="pyrD"
                     /locus_tag="PLN02_09025"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HOI52506.1"
                     /translation="ATTPIEKAADDYLICLDKVYGDASYVAVNISSPNTRNLRELQKD
                     EALDDLLSQLKARQQALAQQHGRYVPMALKIAPDLDDAQIATIADALRRHRFDGVIAT
                     NTTLSREGVDHLPNGNEGGGLSGAPVFRKSTDVQHKLATALAGEVPIIGVGGILKGED
                     AAAKIRAGASLVQVYSGFIYRGPELVAEMGQAVARALAEKKPITA"
BASE COUNT          422 a          778 c          919 g          473 t
ORIGIN      
        1 aactgcttgg cgccgcccgc cggatagagc gccggcaccg ggacgacggc gaagggttcg
       61 gcgagcgccg ccaccgccgc gcgcagggcg gcgatcgctt ccggcttgtt gtcctcgacg
      121 ccgatgatca cctgttgcgg cttgagcatg tcgcggaaga tcgcggtgcc acggacgatt
      181 tcctcggcac gctcgcgcat caggcggtcg tcgcaggtga tgtagggttc gcattcggcg
      241 gcgttgatga tcagttccgc catcgctacc ttcgggttgg cggtcagctt gccgtgcgtc
      301 gggaagccgg cgccgccgag accgacgatg ccgtaacgct gcaggtagct gcagatgtcg
      361 gccggcgtca tcgccgcgta gtcggccgga ccgtgcgcga tccattcgtc gcgtccatcc
      421 ggctcgatga ccacgcacag tgagcccagc ccggaaggat gcgggcgcgt gtgcatggcg
      481 atttcgacga ccgttcccga ggtcggggca tggatggcgg cggacagcca gccgtcggcc
      541 tcgccgatca actggccctt gagcacgcga tcgccgaccg cgaccaccgg tcgcggcgtg
      601 ccgccgatgc tctgatgcag cggtacgatc agccgcgacg gcaacggcgc ggtgacgatg
      661 ggcgcctgat tggagaggtc cttgtggctg gccggcttga cgccgccatg gaacttgaac
      721 aactggctca gcatcaggcg gcctccttct cgggcgaggc ggcggggctg gcaggatgga
      781 tgcttaccac cgggtatttc catttccagt tatccagcgt ttccggcacc acttccatgt
      841 ggatgcattc gaccgggcag ggcggcaggc agagttcgca gccggtgcac tgctgggtga
      901 ggatggtgtg cagttgcttg gcggcgccga cgatggcgtc gaccgggcag gcctggatgc
      961 acagcgtgca gccgatgcag gtgttctcgt cgatgcgggc gacctgcttc ggcttttcct
     1021 cggcttccag cgccttgacc tcgcggccga gcaggtcggc caggcgctgc acgccttcca
     1081 tgccgcctgg cgggcacagg ttgatgtcgg cctcgccctt ggcgatcgct tcggcgtagg
     1141 gcttgcagcc ggggtagccg cactggccgc actgcgtctg tggcaggatg gcgtcgatct
     1201 tttcgaccag cgggtcgccc tcgaccttga acttgatcga cgagaaaccg agcgcggcgc
     1261 cgagcacgag cgcgcccagg gccatgattg caatggcgag caggatgtcc attattgata
     1321 cttgtccagt ccggcgaagc ccatgaaggc cagcgccatc aggccggcgg tgacgagggc
     1381 gatggcggtg ccgcggaagt gggtcggcac gtcggcgcct tcgatgcgtt cgcggatgcc
     1441 ggcgaagagg atcagcacgc cggaaaagcc gaccgcgcta ccggcgccgt agagcagcga
     1501 ttcgacgaag tcgtagccgt tggcgacgtt gagtagcggc acgccgagca ccgcgcagtt
     1561 ggtcgtgatc agcggcaggt agatgcccaa ggtctgctgc agcgtcggcg aggtcttggc
     1621 gatgaccatt tcggtgagct ggacgatggc ggcgatggtg acgatgaagg acagggtgcg
     1681 cagatattcc agcccgaaag gcatgagcag gaagtggtcg atgatgtagc tggaaccggt
     1741 cgccagggtc agcacgaagg tggtcgcggc gcccatcccg taagccgtct ccagtttctt
     1801 ggaaacgccc atgaaggggc acaggccaag gatcttgacg agcacgacgt tgttgaccag
     1861 caccgcgccg acgagaagga aaatgtagtg gctcatgaaa gggggaagga gcgaaggcgg
     1921 ctattatcgg agtttgccgc agcgcgaaca accgcgcgcc gggcagggaa atttatgcgg
     1981 tgatgggttt tttctcggcc agtgcgcggg cgacggcttg gcccatttcg gccaccagtt
     2041 ccggtccacg atagatgaaa ccgctgtaca cctggaccag gctcgctccg gcacggattt
     2101 tcgcggcggc atcctccccc ttgagaatgc caccgacgcc gatgatcggc acttcgccgg
     2161 ccagcgccgt cgcgagcttg tgttgtacgt cggtcgattt gcggaatacc ggcgccccgg
     2221 acaggccgcc gccttcgttg ccgttgggca ggtgatcgac gccctcgcgg gagagcgtgg
     2281 tattggtcgc gatgacgccg tcgaaacggt gccggcgcag tgcgtcggcg atcgtcgcga
     2341 tctgcgcgtc gtcgaggtcg ggcgcgatct tcagcgccat cggcacatag cggccgtgtt
     2401 gctgagcgag cgcctgctgg cgtgctttca gttgcgacag cagatcgtcg agtgcctcgt
     2461 ccttctgcag ttcgcgcagg ttgcgggtgt tcggcgacga gatgttgacg gcaacatagc
     2521 tggcgtcgcc atagaccttg tcgaggcaga tcaggtaatc gtcggccgcc ttctcgatcg
     2581 gcgtcgtcgc gt
//
LOCUS       DAOSDX010000392         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14038_length_2579_cov_3.309429, whole genome shotgun sequence.
ACCESSION   DAOSDX010000392 DAOSDX010000000
VERSION     DAOSDX010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14038_length_2579_cov_3.309429"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLN02_09030"
     CDS             <1..760
                     /locus_tag="PLN02_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOI52507.1"
                     /translation="DPTDVFVPLLIVTYIGTLTGLFVTGFFQKLHLWNRVTLAYLGGA
                     TALIGGLVAYFSALPSAEMTRQSSILSNVVLFGIVVVFVLVFGTISEALGFHFVIGAF
                     FGALFLDQRHFLASRYEDLRGTLASITNGFLAPVFFAYLGLEFQISAISELDFTVVVI
                     LVSILSKLFAGWLGGRIVGMAHREALGLGCVLNGRGVMELVVAGIAYQKGFIDPMIFS
                     TLVLMGILTTFLTPILFKQTFRGPRLERYRTGNH"
     gene            complement(828..901)
                     /locus_tag="PLN02_09035"
     tRNA            complement(828..901)
                     /locus_tag="PLN02_09035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(867..869),aa:Gly,seq:ccc)
     gene            complement(955..1446)
                     /locus_tag="PLN02_09040"
     CDS             complement(955..1446)
                     /locus_tag="PLN02_09040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOI52508.1"
                     /translation="MTQPVTDTRALRNALGRFATGIAVVTAIDPDGKPIGLTVNSFSA
                     VSLQPALVLWCLGSGSANLEAFRKASHHAINILAADQQDLSNRFATWPTDRFAGLPWH
                     GGYGGAPVLEGCCASFEVANESEVPGGDHSIFIGRVERYGENPELAPLLFHAGQYRRL
                     AEL"
     gene            1510..2550
                     /gene="mutY"
                     /locus_tag="PLN02_09045"
     CDS             1510..2550
                     /gene="mutY"
                     /locus_tag="PLN02_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOI52509.1"
                     /translation="MPQEQSFAQRLIAWQRSAGRHDLPWQGTRDPYRVWLSEIMLQQT
                     QVATVIPYYQRFLSSFPDVATLAEAPVERVIEHWAGLGYYARARNLHRCAQRVIAEHG
                     GRFPENAAALAGLPGIGRSTAAAIAAFCFGERAAILDGNVKRVLCRVFGIAGFPGATA
                     VERRLWSLAESLLPAAGEVGTYTQGLMDLGATLCTRGLPNCPACPFAESCVARRENRQ
                     RSLPEAKPRPPLPEREAVFVLILAGDRLLLERRPPHGLWGGLLVPPQGEPAAILAGRG
                     LVAVEQRELPPLKHVFTHFRLTLKPLLCRIAPPRSAGEPGDEWLPLGEAAAAGVPTPI
                     RKLIRQVASAGD"
BASE COUNT          392 a          866 c          869 g          452 t
ORIGIN      
        1 cgatccgacc gacgtgttcg tgccgctcct gatcgtcacc tacatcggca cgctcaccgg
       61 gctcttcgtc accggcttct tccagaagct gcatctgtgg aaccgcgtca cgctggccta
      121 cctgggcggg gccaccgcgc tgatcggcgg cctggtcgca tatttctccg cgctgccgtc
      181 cgccgagatg acgcggcagt cgtccatcct tagcaacgtc gtgctgttcg gcatcgtcgt
      241 cgtcttcgtg ctggtcttcg gcaccatcag tgaagcgctc ggtttccact tcgtcatcgg
      301 cgccttcttc ggcgccctct tcctcgacca acgccacttc ctcgcctccc gttacgaaga
      361 cctgcgcggc accctggcct cgatcaccaa cggcttcctg gcgccggtct tcttcgccta
      421 tctcggcctg gaattccaga tcagcgcgat cagcgaactg gacttcaccg tcgtcgtcat
      481 cctcgtctcg atcctcagca agctgttcgc cggctggctg ggcgggcgca tcgtcggcat
      541 ggcgcaccgc gaggcgctcg gcctgggctg cgtgctcaac ggccgcggcg tgatggaact
      601 ggtggtggca ggcatcgcct accagaaagg cttcatcgac ccgatgatct tctccacctt
      661 ggtcctgatg ggcatcctga ccaccttcct gacaccgatc ctgttcaagc agaccttccg
      721 cggcccccgc ctcgaacgct accgcacggg aaaccactga gcccggcaac ggcggtggcc
      781 agacaacaaa aagcccaccg acgtcgggtg ggctgggcat gctttggtgg agcgggcgat
      841 gggaatcgaa cccacggctc tagcttggga agctagggta ttaccattat acgacgcccg
      901 ctcgggcggc aaggacgcga ttctatgccg agttcgtgtc cggttcaatc gttgtcagag
      961 ctcggcgagg cggcggtact ggccggcgtg gaagagcagc ggggcaagtt ccgggttttc
     1021 gccgtagcgc tcgacgcggc cgatgaagat gctgtggtcg ccacccggaa cttcgctctc
     1081 gttggcgact tcgaagctgg cgcagcagcc ttccagcacc ggcgcgccgc cgtagccgcc
     1141 gtgccagggc aagccggcga agcggtcggt cggccaggtg gcgaagcggt tggagaggtc
     1201 ctgctggtcg gcggcgagga tgttgatcgc gtgatggctc gccttgcgga aggcttccag
     1261 gttggccgaa ccgctgccca ggcaccagag gaccagcgcc ggttgcagcg agacggcgga
     1321 gaaggagttg accgtcagtc cgatcggctt gccatccggg tcgatggccg tgacgacggc
     1381 gatgccggtg gcgaagcggc cgagggcatt gcgcagggcg cgggtatcgg taacgggctg
     1441 ggtcatgggc gtcgcaggcg aaaaaaggac gtattatccc ggctcccgct ttccgcgtcg
     1501 agacgaaatt tgccgcaaga acaaagcttt gcccaacgcc tgatcgcctg gcagcgcagc
     1561 gccggacgcc atgacctgcc gtggcaggga acgcgcgacc cctaccgggt ctggttgtcg
     1621 gagatcatgc tgcagcagac ccaggtggcg acggtgatcc cttattacca gcgcttcctg
     1681 agcagctttc ccgatgtggc gacactggcg gaagcaccag tggaacgggt gatcgagcat
     1741 tgggccggcc tcggctatta cgcccgagcg cgcaacctgc accgctgcgc gcagcgcgtg
     1801 atcgccgagc acggcggccg ttttccggag aatgccgcgg cgcttgccgg cttgccgggc
     1861 atcggccggt cgacggcggc agcgattgcc gccttctgtt tcggcgagcg ggctgcgatc
     1921 ctcgacggca acgtcaagcg cgtgctgtgc cgggtgttcg ggattgccgg ttttcccggc
     1981 gcgacggcag tcgaacgacg cctgtggtcc ctcgccgaaa gcctcctgcc ggcggctggc
     2041 gaggtcggca cctacacgca gggcctgatg gacctcgggg caacactatg cacgcgcggc
     2101 ctgcccaact gcccggcctg tccctttgcc gaaagctgcg tcgcccgccg cgaaaaccgc
     2161 cagcgcagcc tgccggaggc gaagccgcga ccgccgctgc cggagcgcga ggcggtgttc
     2221 gtgctgatcc tggccggcga ccgcctgctg cttgaacggc ggccgccgca cgggctgtgg
     2281 ggcggcctgc tggtgccgcc gcaaggtgag ccggcggcaa tcctggccgg gcgcgggctg
     2341 gtcgccgtcg agcagcggga gttaccgccg ctcaagcacg tcttcacgca tttccggctg
     2401 accctgaaac cgctgctttg ccgcatcgcg ccgccgcgct cggcgggcga accgggcgac
     2461 gaatggctgc cgctgggcga ggctgccgcc gccggcgtgc cgacgccgat ccgcaagctg
     2521 atcaggcagg ttgccagcgc aggggactga gcccccagcg ctcgaaactt tcggcgcgc
//
LOCUS       DAOSDX010000393         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14095_length_2571_cov_3.000397, whole genome shotgun sequence.
ACCESSION   DAOSDX010000393 DAOSDX010000000
VERSION     DAOSDX010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14095_length_2571_cov_3.000397"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLN02_09050"
     CDS             <1..561
                     /locus_tag="PLN02_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52510.1"
                     /translation="ISKRLIEMMDGRFGVDSTPGVGSTFWFSVDLETGAPGVDAAPKA
                     LEAPEEKLRQRRQRARILLAEDEPINREVSLALLEDAALAVDVAEDGEQALALAGKNA
                     YALILMDIQMPKMNGLDATRAIRRLPQHAQTPILAMTANAFDEDRQTCLAAGMNDHIS
                     KPIDPDRLFATLLRWLPEDAETPPQT"
     gene            complement(552..905)
                     /locus_tag="PLN02_09055"
     CDS             complement(552..905)
                     /locus_tag="PLN02_09055"
                     /inference="COORDINATES: protein motif:HMM:NF025640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HOI52511.1"
                     /translation="MASISAVGGTTAVSASVASSGNASASRIAALQKQLSALQKELSQ
                     LLKDPSEETEKQAEILQAQIAALQAQIAQLQAQEAREAAEAQSEQAKSEPAGAATGAA
                     ASSDDILGTQVDLYV"
     gene            1048..1539
                     /gene="ybaK"
                     /locus_tag="PLN02_09060"
     CDS             1048..1539
                     /gene="ybaK"
                     /locus_tag="PLN02_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOI52512.1"
                     /translation="MSKAAHAPETQATQFLKANKVAYAPHLYAYEEHGGTRVSARELG
                     VDEHAVVKTLIFEDENGKPLIVLMHGDCKVSTKELARQIPCKKVEPCKPEVANRHTGF
                     LVGGTSPFGTKKAMPVFIEQSILDLPLIYINGGRRGFLVALDPKEILRTLHPKCVEAA
                     LGD"
     gene            1896..>2571
                     /locus_tag="PLN02_09065"
     CDS             1896..>2571
                     /locus_tag="PLN02_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOI52513.1"
                     /translation="MTQLVARHLAHPGLRRRWLAGVALAAGALLFGIDQAITWFAAQP
                     MAWHGLQASLLAGLATGIGALPVLFLRRPNEGLMAPMLGLAAGMMLAASLFSLLVPAV
                     ETVAASGAPWTLGCLTALALLAGAAIMHGIDRRTAHAHVEDIDSRQDLPRIGLVVAAI
                     ALHNVPEGLAVGVAAASGVDHGMSLGIAIQNVPEGWIVASAMLVLGAGPWRAALMALA
                     TGLVEPV"
BASE COUNT          423 a          929 c          804 g          415 t
ORIGIN      
        1 atcagcaagc ggctgatcga aatgatggac ggccggttcg gcgtggacag cacgccgggc
       61 gtcggcagca ccttctggtt cagcgtcgac ctcgaaacgg gggcgccagg agtggacgcc
      121 gcccccaagg cgctggaagc tcccgaggaa aaacttcgac agcgacgcca gcgcgcccgc
      181 atcctgctcg ccgaggatga accgatcaac cgcgaggtct cgctggcgct gctcgaagac
      241 gccgcgctcg ccgtcgatgt cgcggaggac ggcgaacagg cgctggccct ggccggcaag
      301 aacgcctacg cgctgatcct gatggacatc cagatgccga aaatgaatgg cctcgacgcc
      361 actcgcgcca tccgccgctt gccgcagcac gcgcagacac cgatcctggc gatgaccgcc
      421 aacgccttcg acgaggatcg ccagacctgc ctggccgccg gcatgaacga ccacatcagc
      481 aagccgatcg accccgaccg gctgttcgcc acgctgctgc gctggctgcc ggaagacgca
      541 gaaacgccgc ctcagacgta gagatcgacc tgcgtgccga ggatgtcgtc gctactcgcc
      601 gccgcccccg ttgccgcgcc agccggttcg ctcttcgctt gttcgctctg ggcctcggcg
      661 gcttcccgcg cctcctgcgc ctgcaactgg gcgatctgcg cttgcagggc ggcgatctgc
      721 gcctgcagga tttcggcctg cttttcggtt tcctcggacg gatccttgag caactgactc
      781 aattccttct gcagcgccga caactgtttc tgcagcgcgg cgatgcgcga cgccgaagca
      841 ttccccgaac tggcgacact ggcgctgacc gccgtcgtgc cgccgaccgc actgatcgat
      901 gccatgatgt tctccctgtg tcattttcct gtctacattt acggcaccgg cgagcgcagc
      961 ttgtgccatc ggcgggtaaa agcgggtaaa gagcgccggc ccgccggcca cgctagaatt
     1021 gcgcctcccg acccgaagcg caatccgatg agcaaagccg cccacgcccc cgaaacccag
     1081 gccacccagt tcctcaaggc gaacaaggtg gcctacgcgc cgcatctcta cgcctacgag
     1141 gaacacggcg gcacccgcgt ctcggcccgc gaactcggcg tcgacgagca cgccgtggtg
     1201 aaaacgctga tcttcgagga cgagaacggc aagccgctga tcgtgctgat gcatggcgac
     1261 tgcaaggtat cgaccaagga gctggcgcgg cagattccgt gcaagaaggt cgagccgtgc
     1321 aaacccgagg tcgccaaccg ccacaccggc ttcctggtcg gcggcaccag ccccttcggc
     1381 acgaaaaagg cgatgccggt gttcatcgag cagagcatcc tcgacctgcc gctgatctac
     1441 atcaacggcg gccgccgcgg cttcctggtc gccctcgacc cgaaggaaat cctgcgcacg
     1501 ctgcacccga agtgcgtcga ggccgccctc ggcgactgat ttttagggcg tgttcacagt
     1561 aagcagcggg cggcgcaagg ggttggcggg gaggcagtgc aaggcgccgg tcgcgccgtg
     1621 cagtgatctg cacaagcgag cggcaacgcg gcaatgcgcc ccgccaaccc cttgcccttc
     1681 gggtttgccg tggcgggcgg tcgccggcgt tactcggctc gggaatggaa caaccattcc
     1741 cagcgcctcg cgccttggcg cccttcccgc cacggcaaac gccgtcccgc tggttactgt
     1801 gaacacgccc tagctatcaa atcgatttaa gcaatcgatt ggagctatca aggcagcggc
     1861 cataaagttc ttccgtcagc caccggagaa ccgccatgac ccagctcgtc gcccgtcacc
     1921 ttgcccatcc cggcctccgc cgccgctggt tggccggcgt cgcgctcgcc gccggcgccc
     1981 tgctgttcgg catcgaccag gccatcacct ggtttgccgc ccagccgatg gcctggcacg
     2041 gccttcaggc cagcctgctg gccggtctcg ccaccggaat cggggcgctg ccggtactct
     2101 tcctgcgccg cccgaacgaa ggcctgatgg cgccaatgct cggcctcgcc gccggcatga
     2161 tgctagccgc cagcctcttt tcgctgctcg tgccggcggt cgagacggtc gccgccagtg
     2221 gcgcgccgtg gacgctcggc tgcctcaccg cgctcgccct gctggccggc gccgccatca
     2281 tgcatggcat cgaccggcgc accgcgcacg cccatgtcga ggacatcgac agtcgccagg
     2341 acttgccgcg gatcggcctg gtggtcgccg cgatcgccct gcacaacgtc ccggagggcc
     2401 tcgccgtcgg cgttgctgcc gcgtccggcg tcgatcacgg aatgtcgctc ggcattgcca
     2461 tccagaacgt gccggaaggc tggatcgtcg ccagcgccat gctcgtgctt ggcgccggcc
     2521 cctggcgcgc cgcgctgatg gccttggcga ccggtctcgt cgagccggtc g
//
LOCUS       DAOSDX010000394         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14153_length_2562_cov_3.131233, whole genome shotgun sequence.
ACCESSION   DAOSDX010000394 DAOSDX010000000
VERSION     DAOSDX010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14153_length_2562_cov_3.131233"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLN02_09070"
     CDS             complement(<1..667)
                     /locus_tag="PLN02_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI52514.1"
                     /translation="MRLLIVEDNSDILANIIDYLEAAGHVVDCARNGPGGLHLALTGH
                     YDLIVLDIMLPGMDGYQLCRSLRDAGCQVPVIMLTARDALDDRLQGLRSGADDYLVKP
                     FALAELDARIEAILRRTRGASQRLQVADLVLDLDTWRATRAGQPLRLNPACLKLLETL
                     MRKSPGVVRREALETALWGDDPPASDSLRSHLHLLRQAVDKPFPRPLLHTVHGVGYRL
                     GEEA"
     gene            844..>2562
                     /locus_tag="PLN02_09075"
     CDS             844..>2562
                     /locus_tag="PLN02_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOI52515.1"
                     /translation="MPFSRYAQPRFLTLVLACWLLVFFAVRGLLLATHFAEADAGFLA
                     ILRLFAVGTVYDLAFLVYAALPLALYLLLCPERMWRSRGHRAFLLVLTGIGFFAMLFV
                     AAAEWLFWDEFGVRFNFIAVDYLVYSKEVVDNIVESYPVYRLLGVLAIVAGVATLALK
                     RQLIASFDAPSLPRRQALLGFAGLLLAAGLNLAVVGQSFPHGEGGNVYQRELASNGPF
                     QFFAAFRNNELDYRQFYATLPDAEAGALLRAEVAEPNARFLGDHPLDIRRAIDNPGPE
                     RRHNIVLVTIESLSAKYLGSFGDPRGLTPNFDRLRGESLFFSNFRATGTRTDRGLEAI
                     TLSMPPTPGRSIVKRQGRESGYASLGQQLTARGYDSVFVYGGRGYFDNMNAFFSGNGY
                     RIVDQSSVPESEMSFQNAWGMADEDIYRQALKIGDQDHAAGTPFFLQLMTTSNHRPYT
                     YPDGRIDIPSGDGREGAVKYTDHAIGQFLEQARSKPWFADTVFVFVADHTAGSAGYED
                     LPVANYHIPLFIYAPGLIKPREMTGLASQIDLAPTLLGLLGIDYESTFYGRNLLLDPQ
                     RPGRAVI"
BASE COUNT          409 a          950 c          759 g          444 t
ORIGIN      
        1 gggcctcttc cccgagacga tagccgacgc catgcacggt atgcagcagc ggccgcggga
       61 agggcttgtc caccgcctgg cgcagcaggt gcaggtggct gcgtaggctg tcgctggccg
      121 gcggatcgtc gccccacaac gcggtttcca gcgcttcccg gcggacgacg ccgggactct
      181 tgcgcatcag cgtctcgagc agcttgaggc aggccgggtt gagacgcagc ggctggccgg
      241 cgcgggtcgc ccgccaggtg tcgagatcaa gcaccaggtc ggcaacctgc aggcgctggc
      301 tggcgccgcg cgtgcggcgc aggatcgcct cgatgcgcgc atcgagttcg gccagcgcaa
      361 aaggcttcac caggtagtcg tcggcgccgc tgcgcaagcc ctgcaagcgg tcgtcgaggg
      421 catcgcgggc ggtgagcatg atcaccggca cctggcagcc ggcgtcgcgc aggctgcgac
      481 agagctggta gccgtccatg ccgggcagca tgatgtcgag gacgatcagg tcgtaatgcc
      541 cggtcagcgc cagatgcagt ccgcccggcc cgttacgcgc gcaatcgacg acatggccgg
      601 cggcttcgag gtagtcgatg atgttggcga ggatgtcgga attgtcttcg acgatcagca
      661 gacgcatgcg cttggaactt ccggttgcga aatagagagt atagcgatgg cggctgaatc
      721 gcagcttaat cgcccgtctg ccggaagcgc gaggtttctt gacgtttttt tgacaatccg
      781 ttcacgcgcc attgacgttc ccgatcgcag actgcgcacc gtcacagatt ctccgcgcca
      841 ccgatgccct tttcccgtta cgcccagccc cgcttcctca cgctcgtcct cgcctgctgg
      901 ttgctggttt tcttcgccgt ccgcggcctc ctgctcgcca cccatttcgc cgaagccgac
      961 gccggtttcc tggcgatcct gcgcctgttc gcggtcggca ccgtgtacga cctggctttc
     1021 ctggtttacg ccgcgctgcc gctcgccctc tacctgctgc tctgcccgga acgcatgtgg
     1081 cgcagtcgcg gccaccgcgc cttcctgctc gtcctgaccg gcatcggctt cttcgccatg
     1141 ctcttcgtcg ccgccgccga gtggctgttc tgggacgaat tcggcgtccg cttcaacttc
     1201 atcgccgtcg actacctggt ctattccaag gaagtcgtcg acaacatcgt cgagtcctac
     1261 ccggtctatc gcctgctcgg cgtgctcgcc atcgtcgccg gcgtcgccac gctggccttg
     1321 aagcgccagc tcatcgccag cttcgacgcg ccgtcgctgc cgcgccgcca ggcattgctg
     1381 ggcttcgccg gcctgctgct ggccgccggc ctcaacctcg ccgtggtcgg ccagtccttc
     1441 ccgcacggcg agggcggcaa cgtctaccag cgcgaactgg cgagcaacgg cccgttccag
     1501 ttcttcgccg ccttccgcaa caacgaactc gactaccgcc agttctacgc caccctgccc
     1561 gacgccgagg ccggcgccct gctgcgcgcc gaagtcgccg agccgaatgc ccgtttcctc
     1621 ggcgaccacc cgctcgacat ccgccgcgcc atcgacaacc cgggcccgga acgccgccac
     1681 aacatcgtcc tcgtcaccat cgagagcctc agtgccaagt acctcggcag cttcggcgac
     1741 ccgcgcggat tgaccccaaa cttcgaccgc ttgcgcggcg aaagcctgtt cttcagcaat
     1801 ttccgggcga ccggcacgcg caccgaccgc ggcctggaag cgatcaccct gtcgatgccg
     1861 ccgacccccg gccgctccat cgtcaaacgt cagggtcgcg aaagcggcta cgccagcctc
     1921 ggccagcaac tcaccgcgcg cggctacgac agcgtcttcg tctatggcgg acgcggctac
     1981 ttcgacaaca tgaacgcctt cttcagcggc aacggttacc gcatcgtcga ccagagcagc
     2041 gtcccggaaa gcgaaatgtc cttccagaac gcctggggca tggccgacga ggacatctac
     2101 cggcaggcgc tgaagatcgg cgaccaggac cacgccgccg gcacaccctt cttcctgcag
     2161 ttgatgacca cctccaacca ccgcccctac acctacccgg acggccgcat cgacattccc
     2221 tcgggtgacg gccgcgaggg cgccgtcaaa tacaccgacc acgccatcgg ccagttcctc
     2281 gaacaggccc gcagcaagcc gtggtttgcc gacaccgtct tcgtcttcgt cgccgaccac
     2341 accgccggca gcgccggcta cgaggacctg ccggtggcca actaccacat ccccctgttc
     2401 atctacgcac cgggcttgat caaaccgcgc gaaatgaccg ggctggccag ccagatcgac
     2461 ctggcgccaa ccctgctcgg cctgctcggc atcgattacg aatcgacctt ctacggccgc
     2521 aacctgctcc tcgacccgca gcgcccgggc cgcgcggtga tc
//
LOCUS       DAOSDX010000395         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14171_length_2559_cov_3.029553, whole genome shotgun sequence.
ACCESSION   DAOSDX010000395 DAOSDX010000000
VERSION     DAOSDX010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14171_length_2559_cov_3.029553"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..417)
                     /locus_tag="PLN02_09080"
     CDS             complement(115..417)
                     /locus_tag="PLN02_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52516.1"
                     /translation="MKRDANCPLCGAKLPSSAWLDACDELLDGEHGILAARCPACQGR
                     LEALAQTGSLQLGYRNGNNFTAALSLDYPGLQVEIADGRLHLRAEEREWVFVEAED"
     gene            660..1118
                     /locus_tag="PLN02_09085"
     CDS             660..1118
                     /locus_tag="PLN02_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOI52517.1"
                     /translation="MKRKLLLALVATATVATAHAQVKVEDAIKWRQSAYATMGWSMGR
                     IKANLDGTYNKDEVVKAANVIQAIANSGMGALYVPGSDKGKGWAETRVKPELFTDGAK
                     VGKLAGDFNQAANELAKVAASGDAAAVKTAFGKVGGSCKACHDDYRKDNK"
     gene            1204..1896
                     /locus_tag="PLN02_09090"
     CDS             1204..1896
                     /locus_tag="PLN02_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOI52518.1"
                     /translation="MNSKKIRVWDLPTRLFHWLLVLAVLALVVTGKVGGNLIDWHGRI
                     GVGLAGLIAFRLVWGVAGSTYARFGQFFPTPARISAYLRRGHRGVGHNPLGALSVFAL
                     LGLLSIQLLTGLVANDDIAFVGPLFDLVGRDLSNVATGWHKAVVNLLILLVALHIAAI
                     VFYARVKKDNLVKPMLTGWKEVNEGETAQGGGWLALLVALAIAGLAVYLASGSWLPEP
                     PPPPPAVETPAW"
     gene            1980..>2559
                     /locus_tag="PLN02_09095"
     CDS             1980..>2559
                     /locus_tag="PLN02_09095"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOI52519.1"
                     /translation="MSYRVIVALASLLLMSVFAGVALFGARLADGRLAESLESEARRL
                     KTAYELSQGELEQQMMALAGTIAADPETIRLMRAAGEAVAAEGGAAGGARAAAVRAAL
                     AAHRQQQWDYLQREVGVRQMQYLLPDLTSFLRFHAPTQFGDSLGELRYLLRNVEELRQ
                     VASGFETGRAFAGIRGAAPVLDRLPDGRENLVG"
BASE COUNT          436 a          755 c          868 g          500 t
ORIGIN      
        1 cttctttggc tactttcttc ttggcgaaac aagaagaaag tacgcccgcc ggtcagcggc
       61 ggaacccaac ggttaatcaa ccaaagacgc ccacgcgtca ggtgcggaac ccgctcagtc
      121 ctccgcttcg acaaataccc actcccgctc ctccgcccgc aggtggagcc gaccgtcggc
      181 gatttcgacc tgtagtccgg gatagtcgag gctgagcgca gccgtgaaat tgttgccgtt
      241 gcggtagccg agttgcaggc tgccggtttg cgccagggct tcgaggcggc cctggcaggc
      301 cgggcagcgg gcggcgagga tgccgtgttc gccgtcgagc agttcgtcac aggcgtcgag
      361 ccaggccgag gagggaagct tggcgccgca gagcgggcag ttggcgtcac gtttcatgcg
      421 tactccggca ggatgttgtt gcgccagctt agcgcggaag tcgatttggc gctgcgttgc
      481 gggcccggtt caggtttgcc attggcctat cgttcgaatt cgttcaacgt tttcgtgaat
      541 atcactcagc gagccttctc tccggagcct gggcgggcgc gtataatcgc cggggaactt
      601 cgggggagta gtggttcgct caattaatca ttttccttca acacaacgga gattacacga
      661 tgaaacgcaa gttgctgcta gccttggtcg ccaccgcaac cgtcgccacc gctcacgctc
      721 aggtcaaggt cgaggatgcc atcaagtggc gccagtcggc atatgccacg atgggctgga
      781 gcatgggtcg gatcaaggcc aacctcgacg gtacctacaa caaggatgag gtggtcaagg
      841 ccgccaacgt cattcaggcg atcgccaatt ccggcatggg cgcgctttat gtgccgggca
      901 gcgacaaggg taagggctgg gccgaaaccc gcgtcaagcc cgaactgttc accgatggtg
      961 ccaaggtcgg caaactggcc ggcgatttca atcaggctgc caatgaactg gccaaggttg
     1021 ccgccagcgg cgacgcggca gcggtgaaga cggcgttcgg caaggtcggc ggtagctgca
     1081 aggcctgtca cgacgattac cgcaaggaca acaagtaagg ttccggcgtt ctgaacgcag
     1141 ggatcggggc gccgcgaggc gcccttgtcg tttggcgcga aggaatacga aaagaggtat
     1201 gcaatgaaca gcaagaaaat ccgggtctgg gatctgccga cccgtctctt ccactggcta
     1261 ctggtgctgg cggtgctcgc tctggtggta accggtaagg taggcggcaa cctgatcgac
     1321 tggcatggca ggattggcgt cgggctcgcc ggactgatcg ccttccgcct ggtctggggc
     1381 gtcgccggct cgacctatgc ccgcttcggc cagttcttcc cgacgccggc gcggatttcc
     1441 gcctacctgc gccgcggcca tcgtggcgtt ggccataatc cgctgggggc cttgtcggta
     1501 tttgccctgc tcggcctgct gagcattcag ttgttgaccg gcctcgtggc caacgatgac
     1561 atcgccttcg tcggcccgct cttcgatctg gtcggccgtg atctgagcaa cgtggcgacc
     1621 ggctggcaca aggccgtggt caacctgctg atcctgctgg tggcgctgca catcgcggcg
     1681 atcgtcttct atgcccgggt gaagaaggac aacctggtca agccgatgct caccggctgg
     1741 aaggaagtga acgagggcga gacagcgcaa ggtggaggct ggctggcgct gcttgtcgcc
     1801 ctggcgattg ccgggctcgc cgtgtacctg gccagcggta gctggttgcc ggagccgccg
     1861 ccaccgccac cggctgttga aaccccggct tggtgagact tttcggcaaa aaacttcaat
     1921 tgcgaccgga gggccggtgc ctataatgga ccgagttcat tttctgccat ccggaagcca
     1981 tgtcctatcg cgtcatcgtc gcgttggcca gtctgttgct gatgtcggtt tttgccggtg
     2041 tcgcgctgtt tggcgcgcgc ctcgccgatg gtcgtctggc ggaaagcctg gagagcgaag
     2101 cgaggcgcct gaaaacagct tacgagttgt cgcagggcga actcgagcag cagatgatgg
     2161 cgctggccgg gacgatcgcc gccgatccgg agacgatccg cctgatgcgt gcggctggcg
     2221 aggcggtggc ggccgagggc ggcgctgccg gcggtgcgcg ggcggccgcg gtgcgcgccg
     2281 cgttggcggc gcaccggcag caacagtggg actacctgca acgcgaggtc ggcgtccggc
     2341 agatgcagta tctgttgccg gatctcactt ccttcctgcg tttccatgcg ccgacgcagt
     2401 tcggcgacag cctgggcgaa ctgcgttacc tgttgcgtaa cgtcgaggag ttgcggcaag
     2461 tggcgagcgg tttcgagact ggccgcgctt ttgccggcat ccgcggggcg gcgccggtgc
     2521 tcgatcgttt gccggacggg cgcgagaatc tcgtcggct
//
LOCUS       DAOSDX010000396         2548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14246_length_2548_cov_3.340554, whole genome shotgun sequence.
ACCESSION   DAOSDX010000396 DAOSDX010000000
VERSION     DAOSDX010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14246_length_2548_cov_3.340554"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLN02_09100"
     CDS             <1..227
                     /locus_tag="PLN02_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604401.1"
                     /note="sigma 54-dependent; regulates genes involved in
                     forming a 2',3'-cyclic phosphodiester on RNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HOI52520.1"
                     /translation="VLGELAEQLDLFERCQLNTVIDTCRNSPSLSEAGRRLFAISRAG
                     KKQSNDADRLRKYLARFALDFETLKNGARR"
     gene            complement(245..1036)
                     /gene="cobA"
                     /locus_tag="PLN02_09105"
     CDS             complement(245..1036)
                     /gene="cobA"
                     /locus_tag="PLN02_09105"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOI52521.1"
                     /translation="MRAIEREVFGKVYLVGAGPGDPELLTLRAARLLARAEVVVYDHL
                     VGPGILDLLPASARRVYAGKEAGHHALPQEETNRLLIELAREGMSVIRLKGGDPFIFG
                     RGGEEMEALEAAGIACEIVPGITSAAGVGAYAGIPLTHRDHARTLIFTTGHLKDGSPD
                     LDWPALARPQQTVVIYMGLGALAIICQRLIEHGLDPATPGVVVQSASTSAQRTVHASI
                     AELPDAVRDAGLKSPSLIVIGPVTGLYRPERERECCIAARVVSVV"
     gene            1224..>2548
                     /locus_tag="PLN02_09110"
     CDS             1224..>2548
                     /locus_tag="PLN02_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOI52522.1"
                     /translation="MKRNKWLQRSLGLAMLPLAIGVAFADNAKKDAADVQYHAGGSPL
                     ANVEMYQDINPKAPPMTKAEFDKARRIYFERCAGCHGVLRKGATGKPLTPDITLDKGT
                     DYLKVFIQYGSPAGMPNWGTSGELTDAEVDLMARYVQQTPPQPPEYGMAEMKNTWKVI
                     VPPEKRPTKKMNSYNLDNIFSTTLRDSGEIALIDGDSKQIINIVKTGYAVHISRTSAS
                     GRYLYVIGRDAKINLIDLWMPKPDNVAEVRIGLEARSVETSKAKGYEDKYAIAGAYWP
                     PQFTIMNGDTLEPLKIVGTRGMTVDTQEYHPEPRVASIVASHFKPEFVVNVKETGKIL
                     MVDYSNINALKTTEIGAARFLHDGGWDASKRYFMVAANQSNKVAAVDAKDGKLAALIE
                     VGKIPHPGRGANFVHPKFGPVWATGHLGDETVSLIGTDPQKHPKQAWKVV"
BASE COUNT          500 a          873 c          806 g          369 t
ORIGIN      
        1 aggtgctcgg cgagcttgcc gagcagctcg acctgttcga gcgctgccag ttgaacaccg
       61 tgatcgacac ctgccgcaac agccccagcc tttccgaggc cggccgccgc ctgttcgcga
      121 tttcccgcgc cgggaagaaa cagagcaacg acgccgaccg cctgcgcaaa tacctcgccc
      181 gtttcgcgct cgatttcgag acgctgaaga acggcgcccg ccgctgagct gacgccgccc
      241 ccgctcagac gacgctgacg acgcgcgcgg cgatacagca ctcgcgctcg cgttccggcc
      301 ggtagagccc ggtcaccggg ccgatgacga tcagcgatgg cgatttgagt ccggcgtcgc
      361 gcaccgcgtc gggcagttcg gcgatgctcg cgtgcaccgt gcgctgcgcc gaggtgctgg
      421 ccgactggac gacgacgcct ggcgtcgccg ggtcgaggcc gtgttcgatc aggcgctggc
      481 agatgatggc gagcgccccc agccccatgt agatcaccac cgtctgctgc ggccgggcga
      541 gcgccggcca gtcgaggtcg gggctgccgt ccttgaggtg gccggtggtg aagatcaggg
      601 tccgggcgtg atcgcggtgg gtcagcggga tgccggcgta ggcgccgacg ccggccgccg
      661 aggtgatgcc gggaacgatc tcgcaggcga tgccggcggc ttccagcgcc tccatctcct
      721 cgccgccgcg gccgaagatg aagggatcgc cgcccttcag ccggatcacg ctcataccct
      781 cacgcgccag ctcgatcagc aggcggttgg tttcctcctg cggcagcgcg tggtggccgg
      841 cttccttgcc ggcatagacg cgacgcgcgc tggccggcag cagatcgagg atgcccgggc
      901 cgaccagatg gtcatagacc accacctcgg cccgggcgag cagccgggcg gcgcgcaggg
      961 tcagcagctc cgggtcgccc ggaccggcgc cgaccaggta gaccttgcca aaaacctctc
     1021 gttcgatagc cctcattttt ctcctccgcg atgccgatcc ggcggctgga cgatgcctgc
     1081 cggccagcga cgaaaccact ctatctgctc gccggacgga aaaaattaag agacatcaag
     1141 gatttccccg gcatgaaaaa aagatactgc ccgcaccgtc aataacggca gcgtgtttcg
     1201 gagcggttct caaggagagg aaaatgaaac ggaataaatg gttgcaacgg tcgctggggc
     1261 tggcaatgct tcccctggcc atcggcgtcg ccttcgcgga caacgcgaag aaggacgccg
     1321 cggatgtgca gtaccacgcc ggcggttcgc cgctggccaa cgtcgagatg tatcaggaca
     1381 tcaacccgaa ggcgccgccg atgaccaagg cggaattcga caaggcccgc cgcatctact
     1441 tcgaacgctg cgccggctgc cacggcgtgc tgcgcaaggg cgccaccggc aagccgctga
     1501 ccccggacat caccctcgac aagggcaccg actacctgaa ggtcttcatc cagtacggtt
     1561 cgccggccgg catgccgaac tggggcacct ccggcgaact gaccgacgcc gaagtcgacc
     1621 tgatggcccg ctacgtgcag cagaccccgc cgcagccgcc ggaatacggc atggccgaga
     1681 tgaagaacac ctggaaggtg atcgtcccgc cggaaaaacg gccgaccaag aagatgaaca
     1741 gctacaacct ggacaacatc ttctcgacga cgctgcgcga cagcggtgaa atcgccctga
     1801 tcgacggcga cagcaagcag atcatcaaca tcgtcaagac cggctacgcc gtgcacatct
     1861 cgcgcacctc ggcgtccggc cgctacctgt acgtgatcgg ccgcgacgcc aagatcaacc
     1921 tgatcgacct gtggatgccg aagccggaca acgtcgccga agtgcgcatc ggcctcgagg
     1981 cgcgctcggt cgaaacttcg aaggccaagg gctacgagga caagtacgcg atcgccggcg
     2041 cctactggcc gccgcaattc acgatcatga acggcgacac cctggagccg ctgaagatcg
     2101 tcggcacccg cggcatgacc gtggacaccc aggaatacca tccggagccg cgcgtcgcct
     2161 cgatcgtcgc ctcccacttc aaaccggagt tcgtggtcaa cgtcaaggaa accggcaaga
     2221 tcctgatggt cgattacagc aacatcaacg ccctcaagac caccgagatc ggcgccgccc
     2281 gcttcctgca cgacggcggc tgggacgcct cgaagcgcta cttcatggtc gccgccaacc
     2341 agtcgaacaa ggttgccgcg gtcgacgcca aggacggcaa gctggccgcc ctgatcgaag
     2401 tcggcaagat cccgcacccg ggacgcggcg ccaacttcgt ccatcccaag ttcggcccgg
     2461 tgtgggcgac cggccatctc ggcgatgaga cggtcagcct gatcggcacc gatccgcaga
     2521 agcatccgaa gcaggcctgg aaggtcgt
//
LOCUS       DAOSDX010000397         2542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14284_length_2542_cov_3.791717, whole genome shotgun sequence.
ACCESSION   DAOSDX010000397 DAOSDX010000000
VERSION     DAOSDX010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14284_length_2542_cov_3.791717"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLN02_09115"
     CDS             <1..937
                     /locus_tag="PLN02_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52523.1"
                     /translation="SIKEATDSINTAAREIAQGNQDLSGRTEQQASSLEETASSMEEL
                     TGTVHQNADNARRANDLAGEAQRVAEQGGNVVDQVVVTMSAIQKASARIADIIGVIDG
                     IAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKALIADSVSKV
                     NAGNRLVDQAGATMTEIVSSIQGVARLMAEISEASREQSSGIEQVGRAINQMDEVTQQ
                     NAALVEEAAAAAESLRGQAQRLALAVAAFHLGAEVVPALGLAPANTVAARQSGLRLPG
                     EKKQHAAALTADFSKKMLAANVSVPSGQADDWAEF"
     gene            962..>2542
                     /locus_tag="PLN02_09120"
     CDS             962..>2542
                     /locus_tag="PLN02_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOI52524.1"
                     /translation="MRNNQPVTANEVLLGADTLIVSRTDPKGLITYVNKAFIDISGFA
                     ESELLGQPHNIVRHPDMPVEAFADLWHDLQAGRPWTGMVKNRCKNGDFYWVQATVTPL
                     WENDRLVGYLSVRRRPTREQVNAAESAYRLFRERRAGGLRIRHGVVVNGGPGWFAGWS
                     LRQKLFAGFATLLLATALVAGIGAWGAGRNYDSAVRINDDGVLPIQSLAVIGRLMAEN
                     RSQIMLSLQHNPEGGYASLHDHDIQFHLRQVDANITEISRQWEIYQQHVEAGAQQELA
                     AEFQQARERFVQEGLLPAKAALLEGRFDDANLILLNKINPAYTKAAAKADALFQAREE
                     GARALVAHSQALYAATLAWSIGLTVLALALGLWIAVAITRSITRPIGEVIAAFRELAN
                     GNYTSKVDVSRDDEMGKVLQGLMSMQIQQGFSVAEAQQLANDNLRIRIALDCVNANLR
                     IADDDGTVIYANKGLQRTLRRIEPALRERQPNFSVDRFIGSSIGDFYADPQAALRGLR
                     ELQNTRNTEMEIGGRIFNV"
BASE COUNT          499 a          800 c          835 g          408 t
ORIGIN      
        1 ctcgatcaag gaagccaccg actcgatcaa caccgcggcc cgcgagatcg cccagggcaa
       61 ccaggatctc tccggtcgca ccgaacagca ggcgtcaagt ctggaagaga cggcgtcgag
      121 catggaagag ttgaccggca ccgtgcatca aaatgccgac aatgcgcggc gcgccaacga
      181 cctggcgggc gaagcgcagc gcgtcgccga acaggggggc aacgtcgtcg accaggttgt
      241 ggtgacgatg agcgccatcc agaaggcgag tgcccggatc gccgacatca tcggggtgat
      301 tgacgggatc gccttccaga ccaatatcct cgccctcaat gccgcggtcg aggcggcgcg
      361 ggcgggcgag cagggccggg gcttcgcggt ggtggcgacc gaggtgcgta gcctggcgca
      421 gcgcagtgcc gccgcagcca aggagatcaa ggcgctgatc gccgattcgg tcagcaaggt
      481 caatgccggc aaccggctgg tcgatcaggc cggtgcgacg atgaccgaga ttgtttccag
      541 cattcagggc gtggcgcggc tcatggcgga aatctccgaa gccagccgtg agcagagctc
      601 cggcatcgag caggtgggac gcgcgatcaa ccagatggac gaagtaaccc aacaaaatgc
      661 cgcattggtc gaggaagccg ccgccgccgc cgaaagcctg cgcggtcagg cgcagcgact
      721 ggcgctggcg gtggcggcct tccatttggg ggccgaggtg gttccagcac tcgggctggc
      781 accagcaaac acggtggcag cgcgtcagtc agggctgcgc ttgccgggcg aaaaaaaaca
      841 acacgcggcg gcgctgaccg ctgatttttc caaaaaaatg ctcgccgcca atgtctccgt
      901 gccctccggc caggctgacg actgggccga gttctaatca ttccccagga aaggcagatt
      961 catgcgcaac aaccaacctg tcaccgccaa cgaggtgctc ctgggcgccg ataccctgat
     1021 cgtctcccgg accgatccga agggactgat cacctacgtc aacaaggcat tcatcgatat
     1081 cagcggtttt gccgagtcgg aactgctcgg ccagccgcat aacatcgttc gccatcccga
     1141 catgccggtc gaggcttttg ccgatctctg gcacgatctg caggccggcc ggccgtggac
     1201 cggcatggtc aagaaccgct gcaagaacgg cgatttctat tgggtccagg cgaccgtcac
     1261 gccgttgtgg gagaacgatc ggctggtcgg ttacctgtcg gtacgacggc ggccgacgcg
     1321 cgaacaggtg aacgcggcag aaagcgccta tcgcctgttc cgcgaacggc gcgccggcgg
     1381 cctgcggatt cgccatggcg tcgtggtcaa tggcggccca ggctggtttg ctggctggtc
     1441 gttgcggcag aagctgtttg ccggctttgc caccttgctg ctggcgaccg ccctggttgc
     1501 cgggatcggt gcctggggcg ccgggcgcaa ttacgacagc gcggtgcgca tcaatgacga
     1561 cggcgtcctg ccgatccagt cgctggcggt gatcggccgc ctgatggcgg aaaaccgcag
     1621 ccagatcatg ctgagcctgc agcacaaccc ggaagggggc tacgccagcc tgcacgatca
     1681 cgacatccag ttccatctcc gacaggtcga cgccaacatc accgaaatca gccgacagtg
     1741 ggaaatttat cagcagcatg tcgaagccgg cgcccaacag gaactggctg ccgagttcca
     1801 gcaggcgcgc gagcgtttcg tgcaggaggg cctgctgccg gcgaaggccg ccttgctcga
     1861 ggggcgtttt gatgatgcca acctgatcct gctcaacaag atcaatcctg cctataccaa
     1921 ggccgccgcc aaggcggatg cattgttcca ggcgcgcgag gaaggcgccc gggcgctggt
     1981 cgcccacagt caggcgctgt acgcggcgac gctggcctgg agcattggcc tgaccgtcct
     2041 ggcgctagcc cttggcctgt ggatcgcggt ggcgatcacc cgctcgatca cccggccgat
     2101 cggcgaagtc atcgccgcct tccgcgaact ggcgaacggc aattacacca gtaaggtcga
     2161 tgtttcgcgc gacgacgaaa tgggcaaggt gctgcagggg ctgatgtcga tgcagatcca
     2221 gcagggcttc agcgtcgccg aggcgcagca gttggccaac gacaacctgc gcatccgcat
     2281 tgccctcgat tgcgtcaatg ccaacctgcg catcgccgac gatgacggca cggtgatcta
     2341 cgccaacaag ggcctgcagc gtacgctgcg gcggatcgag ccggcgctgc gcgagcggca
     2401 gcccaacttc tcggtcgatc gtttcatcgg cagcagcatc ggcgatttct atgccgatcc
     2461 gcaggcggca ctgcgcggtc tgcgcgaact gcagaacacg cgcaataccg aaatggaaat
     2521 cggcgggcgg attttcaacg tc
//
LOCUS       DAOSDX010000398         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14354_length_2532_cov_3.082761, whole genome shotgun sequence.
ACCESSION   DAOSDX010000398 DAOSDX010000000
VERSION     DAOSDX010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14354_length_2532_cov_3.082761"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="dgt"
                     /locus_tag="PLN02_09125"
     CDS             <1..1068
                     /gene="dgt"
                     /locus_tag="PLN02_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HOI52525.1"
                     /translation="VESGDFGAIVAAACLAHDIGNPPFGHAGEDAIREWFRNSGLFQR
                     HEFTPAQRADFEKYEGNAQGFRIVSRLQSPANPGGLQLTSAVLATFTKYPRPSALDGP
                     LDGKSGKKFGFFQQDADNFARVAQSTGLVERIPGQAWRRHPLAFLVEVADDTCYLIVD
                     LEDAARLGFVPYKDAECLLADLAGSTINGSRLDRLYDPKERLEYLRAKAIGRLLESAA
                     AVFLENEAAILDGSFDDELLEQSPVSVPLQAVLQVAKETIYTARPALEIETAGFEVLG
                     ALLGLFTHAVEAAAVPELRMTKREEMLLRLLPPQFLGHGGKPDADPYVRLLQVADFVA
                     GMTDSYAVDMYRKLKGFDLPT"
     gene            complement(1069..1374)
                     /locus_tag="PLN02_09130"
     CDS             complement(1069..1374)
                     /locus_tag="PLN02_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52526.1"
                     /translation="MKLQHLAIGDRFEYEGKIFVKTGPLTASSDQGGQQVIPRYAVLK
                     PLDQPQPESRAGGRERLNKAAVLAAFDRFYRTSERLCDSTAHAELARARSEFLAIFE"
     gene            1474..>2532
                     /locus_tag="PLN02_09135"
     CDS             1474..>2532
                     /locus_tag="PLN02_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOI52527.1"
                     /translation="MFGNKYRQQVGELEAALADCHALHAALQRSMAIVELMPDGTVID
                     ANDNFCRTLGYTLSELKGQHHRRLCDPAYAGSPEYDTFWASLRRGEFFRGPVRRRQRD
                     GRDVWLEATYNPVLGPDGRVVKVVKFATDVTPQVIEAASQRAMVDAIERSMAVIEFAT
                     DGRILRANDNFCRAMGYSTRELAGQHHRIFCPEDFVRGPEYAQFWARLGRGEFCSGQY
                     LRLDRQGREVWLEASYNPVFGPDGQVVKVVKFATDISERIRRHMAEREGAETAYKVAL
                     ETRETSQSGEGIILDTVDKMQHIAGIVDESAALVAVLGEQTARISSIVGTIREIADQT
                     NLLALNAAIEAARAGESGR"
BASE COUNT          414 a          837 c          852 g          429 t
ORIGIN      
        1 gtcgaatcgg gcgatttcgg ggcgatcgtc gccgccgcct gcctggccca cgacatcggc
       61 aacccgccct tcggccatgc cggcgaggac gcgatccgcg aatggttccg caattccggc
      121 ctgttccagc gccatgaatt cacgccggcg cagcgcgccg atttcgagaa gtacgagggc
      181 aacgcgcagg ggttccgcat cgtttcgcgg ctgcagagcc cggccaatcc tggcgggctg
      241 cagctgacca gcgcggtgct ggcgaccttc accaagtacc cgcggccttc cgcgctcgac
      301 ggcccgctgg atggcaagag cggcaagaaa ttcggtttct tccagcagga cgccgacaat
      361 tttgcccgcg ttgcccagtc gaccgggctg gtcgagcgaa ttccggggca ggcctggcgc
      421 cgtcatccgc tggccttcct ggtcgaagtg gccgacgata cctgctacct gatcgtcgat
      481 ctcgaagacg cggcgcgtct cggcttcgtg ccctacaagg acgccgaatg cctgctcgcc
      541 gacctcgccg gttcgacgat caacggcagc cggctcgacc gcctgtacga cccgaaggag
      601 cgtctcgaat acttgcgcgc caaggcgatc ggtcgcctgc tggaaagcgc ggcggcggtt
      661 ttcctggaaa acgaggcggc gatcctcgat ggcagtttcg acgacgaact gctcgagcag
      721 tcgccggtca gcgtgccgct gcaggcggtg ctgcaggtgg ccaaggagac gatctacaca
      781 gcgcggccgg cgctggaaat cgaaaccgcc ggcttcgagg tgcttggcgc gctgctcggg
      841 ctgtttaccc acgccgtcga ggcggcggcg gtgcccgaac tgcgcatgac caagcgcgag
      901 gaaatgctgc tccgcctgct gccgccgcaa ttcctcggtc acggcggcaa acccgatgcc
      961 gatccctacg tgcgtctctt gcaggtggcc gacttcgtcg ccggcatgac cgactcctac
     1021 gccgtcgaca tgtaccgcaa gctcaagggt ttcgacctgc cgacctgatc actcgaagat
     1081 ggcgaggaat tcgctgcgcg cccgcgccag ttcggcgtgc gcggtgctgt cgcagaggcg
     1141 ttcgctggtc cggtagaaac ggtcgaaggc ggccagcacc gccgccttgt tcaggcgctc
     1201 gcggccgccg gcccggcttt ccggctgcgg ctggtcgagc ggcttgagca ccgcgtagcg
     1261 aggaatgacc tgctgtccgc cctggtccga ggaggcggtg agcgggccgg tcttgacgaa
     1321 gatttttcct tcgtattcga agcggtcgcc gatggcgaga tgctggagtt tcatgctggg
     1381 tcctgtagcg cggtgcgccg gatattagca aggcgtgtcg cgtccgggcg ggttttggta
     1441 gcatcggcgc cttcaaccag acagggagat ggaatgttcg gcaacaagta ccggcagcag
     1501 gtcggcgaac tcgaagcggc gcttgccgat tgtcacgccc tgcacgcggc gctccagcgc
     1561 tcgatggcga tcgtcgaact gatgcccgac ggcacggtga tcgacgctaa cgacaatttc
     1621 tgccggaccc tcggctacac gctgtccgaa ctcaagggcc agcatcaccg ccgcctgtgc
     1681 gatccagcct atgccggctc gccggaatac gacactttct gggcctcgct gcggcgcggc
     1741 gagttcttcc gcggccccgt gcgccgccgt cagcgcgacg gccgggatgt ctggctggaa
     1801 gccacctaca acccggtgct cgggccggac ggccgggtgg tcaaggtggt caagttcgcc
     1861 accgacgtca cgccgcaggt gatcgaggcc gccagccagc gtgccatggt cgatgccatc
     1921 gagcgctcga tggcggtcat cgaatttgcc accgacggcc gcatcctgcg cgccaacgac
     1981 aatttctgcc gggccatggg ctattcgacc cgcgagctgg ccggccagca tcaccggatc
     2041 ttctgtccgg aagatttcgt gcgcgggccg gagtacgcgc agttctgggc tcgcctgggg
     2101 cgcggcgaat tctgttccgg acagtacctg cggctcgacc gccagggccg cgaggtctgg
     2161 ctggaggcca gctacaaccc ggtgttcggg ccggacgggc aggttgtcaa ggtggtcaag
     2221 ttcgccaccg acatcagcga gcgcatccgt cgccacatgg ccgagcgcga aggcgcggaa
     2281 accgcctaca aggtggcgct ggaaacgcgg gagacttccc agtccggcga gggcatcatc
     2341 ctcgacaccg tggacaagat gcagcacatc gccggcatcg tcgacgaatc ggcggcgctg
     2401 gtcgccgtgc ttggcgaaca gacggcccgc atctcgtcga tcgtcggtac catccgcgag
     2461 atcgccgacc agaccaacct gctggcgctc aatgcggcga tcgaggcggc gcgcgccggc
     2521 gaatcggggc gt
//
LOCUS       DAOSDX010000399         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14436_length_2524_cov_2.632645, whole genome shotgun sequence.
ACCESSION   DAOSDX010000399 DAOSDX010000000
VERSION     DAOSDX010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14436_length_2524_cov_2.632645"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLN02_09140"
     CDS             <1..818
                     /locus_tag="PLN02_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HOI52528.1"
                     /translation="INEINCVRKHLSAIKGGRLALAAWPAPLLTLAISDVPGDDPAVI
                     ASGPTVGDPTTAADALRVLDAYAIPLAAPLRAWLASGAAETPKADDPRLAGATYRLLA
                     SPRQMLEAAAAEARCCGVTPLILGDALEGEAREVGRVHAGIALACARHGFPGAAPCVL
                     LSGGETTVTVRGAGGRGGRNSEFLLGLALALGGAPGIHALAADSDGIDGTEDNAGAFV
                     APDTPLRARSLGLDGQAALAANDAWGYFAALDDLLLTGPTRTNVNDFRAILIA"
     gene            complement(826..2058)
                     /locus_tag="PLN02_09145"
     CDS             complement(826..2058)
                     /locus_tag="PLN02_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOI52529.1"
                     /translation="MLAALEQRRPSIPESEIVAAESFSLNWPNLKTCLAESLGCDDET
                     RCQTMMRQLAATDTPFMLFSYQFGTLRNQLLKLALEDGAIADAQQIMTLFEAMEENFA
                     AVYLEIFLNRLGTRNHLRLSHIRALNDKNLLSYFESHLEWMATLIEATRARDPQRMPE
                     LNPNHCQFGLWLNDEGARLIRDNSHIAEIRELHEAMHHIIAEIDELIVQPRASGPVYA
                     ILKKAETYSLELGNEISLLNSIVIMSVYSKDTLTGFLSRRFLDRVLANQMEIAKATES
                     AFSVIMFDIDHFKDLNDRYGHHTGDLALEHLAGIVRDTLRQSDLIFRYGGEEFLLVCP
                     STRQAQARLLAEKLRLRIEACPVPHDPPLPLSASFGVSEVAPASYEIVDTRLVHEVVA
                     ACDGKLYAAKRGGRNRVV"
     gene            2247..>2524
                     /locus_tag="PLN02_09150"
     CDS             2247..>2524
                     /locus_tag="PLN02_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOI52530.1"
                     /translation="MIPDDTLRCDPATLHRRLRNVLPPHCLLVEDEDLRPYECDGLTA
                     YREMPLAVCLPENEGQVVDVLRTCHQLGVPVVARGAGTGLSGGAMPHA"
BASE COUNT          372 a          812 c          889 g          451 t
ORIGIN      
        1 cgatcaacga aatcaactgc gtgcgcaagc atctctcggc gatcaagggc gggcgcctgg
       61 cgctcgccgc ctggccggca ccgctgctga ccctggcgat ttccgacgtg cccggcgacg
      121 acccggcggt gatcgcttcc gggccgacgg tcggcgatcc gacgacggcg gccgacgccc
      181 tgcgcgtcct cgacgcctac gcgattccgc tcgccgcgcc gctgcgcgcc tggctcgcca
      241 gcggcgccgc cgagacgccg aaggcggacg atccgcggct ggccggcgcc acttaccgcc
      301 tgctcgcctc accgcgccag atgctcgaag cggcggcggc cgaagcgcgg tgttgcggcg
      361 tcacgccact gatcctcggc gacgcgctgg agggcgaggc gcgcgaagtt ggccgagtgc
      421 acgccggcat cgcgctcgcc tgcgcccgcc acggtttccc gggggcagca ccgtgcgtcc
      481 tgctctccgg cggcgagacg acggtgacgg tgcgcggcgc cggcggccgc ggcggacgca
      541 acagcgagtt cctgctcggg ctggcgctgg cgctcggcgg cgcgcccggc atccacgcgc
      601 tggccgccga cagcgacggc atcgacggta ccgaggacaa tgctggcgcc ttcgtcgctc
      661 ccgatacgcc gctgcgggcg cgctcgctcg gcttggacgg tcaggccgcg ctggcggcca
      721 acgacgcctg gggctatttt gcggcgctcg acgacctgtt gctgaccggg ccgacgcgga
      781 ccaacgtcaa cgacttccgg gccatcctga tcgcctgaag cgctttcaga ccacccggtt
      841 gcggccgccg cgcttggcgg cgtagagctt gccgtcgcag gcggcgacca cttcatggac
      901 cagccgggtg tcgacgatct cgtaagaggc cggtgccacc tcgctgacgc cgaagctggc
      961 gctgagcggc agcggcgggt catgcggcac cgggcaggct tcgatgcgca ggcgtagctt
     1021 ctcggccagc aggcgcgctt gcgcctgcct ggtcgatggg cagacgagca ggaactcctc
     1081 gccgccgtag cggaagatca ggtcggactg gcgcagcgtg tcgcggacga tgccggccag
     1141 gtgttcgagc gcaaggtcgc cggtgtggtg gccgtagcgg tcgttgagat ccttgaaatg
     1201 gtcgatgtcg aacatgatca ccgagaacgc cgactcggtc gccttggcga tctccatctg
     1261 gttggccagc acgcggtcga ggaagcgccg gctgaggaag ccggtcagcg tgtccttgct
     1321 gtagacgctc atgatgacga tgctgttgag cagcgaaatc tcgttaccga gttcgagcga
     1381 gtaggtctcc gccttcttga ggatggcgta gaccgggccg ctggcgcgcg gctggacgat
     1441 caactcgtcg atttcggcga tgatgtggtg catcgcctcg tgcagctcgc ggatttcggc
     1501 gatatggctg ttgtcgcgga tcaggcgcgc gccttcgtcg ttcagccaca ggccgaactg
     1561 gcagtgattc gggttgagtt ccggcatgcg ctgcgggtcg cgggcgcggg tggcttcgat
     1621 cagtgtcgcc atccattcga gatggctctc gaaatagctc agcaggttct tgtcgttgag
     1681 cgcgcggatg tgggagagcc gaaggtggtt gcgggtgccg agccggttga ggaagatttc
     1741 cagatagacc gcggcgaagt tttcttccat cgcctcgaac agcgtcatga tctgctgggc
     1801 gtcggcgatc gccccgtctt ccagcgccag cttgagcaac tggttgcgca aggtgccgaa
     1861 ctggtaggag aagagcatga acggcgtgtc ggtggcggcc agctggcgca tcattgtctg
     1921 gcagcgggtc tcgtcgtcgc agcccagcga ttcggcgagg caggtcttga ggttgggcca
     1981 gttgagcgag aagctctcgg cggcgacgat ttcgctctcc gggatgctgg ggcgccgctg
     2041 ctccaatgct gccagcattc tttcgagcaa ttgccggtta gccggcgatc tccattcggc
     2101 catgcgtgcg gttccagtac agagtgagag agggagcgtt ggcgaaatta tattgagccg
     2161 atctatgctg aaagtaaaat ttttgcatgt ccgggcgcgg gttttcccga taggtctttg
     2221 ccgcgttgcg ctttagaatt cggcacatga tccccgacga tacactccgc tgcgaccccg
     2281 cgaccctgca ccgccgcctg cgcaacgtgc tgccgcctca ttgcctgctg gtcgaggacg
     2341 aggatctgcg cccctacgaa tgcgacggcc tcaccgctta ccgcgagatg ccgctggcgg
     2401 tttgcctccc ggagaacgaa ggccaggtgg tcgacgtgct gcgcacctgc caccagctcg
     2461 gcgtgccggt ggtcgcccgc ggcgccggca ccggcctgtc cggcggcgcg atgccgcatg
     2521 ccga
//
LOCUS       DAOSDX010000400         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14440_length_2523_cov_3.309968, whole genome shotgun sequence.
ACCESSION   DAOSDX010000400 DAOSDX010000000
VERSION     DAOSDX010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14440_length_2523_cov_3.309968"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..979)
                     /locus_tag="PLN02_09155"
     CDS             complement(329..979)
                     /locus_tag="PLN02_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI52531.1"
                     /translation="MIVASQLTKRYPGGFEALHGVSFEIRAGELVLITGHSGAGKTTL
                     VKLIASIERPTSGSLVVNGQNLSALRRSAIPYVRRHFGLIFQDQKLLFDRSALDNVLL
                     PLQIAGLPHREAVRRAQAALDKVGLLAREKAYPIALSGGEQQRLAIARAVVNRPTVLL
                     ADEPTANLDRESAQDIRDLFADFHRVGVTVVVATHDQAWVEHCGDNALRLDHGRVV"
     gene            complement(976..1953)
                     /gene="ftsY"
                     /locus_tag="PLN02_09160"
     CDS             complement(976..1953)
                     /gene="ftsY"
                     /locus_tag="PLN02_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOI52532.1"
                     /translation="MFSFLKKSAPDAKTAAPAEVAAAPSWRERLFKGLARTRAQWGGK
                     LKSIFARGKVDDELLEELETLLLTSDVGIEATTHLLDELKKAAKRDKLETPEAIQRAL
                     ADALYETLAPLEQPLDVSTHKPYVIMIAGVNGAGKTTSIGKLAKYFQNQGKSVLLAAG
                     DTFRAAAREQLQTWGERNNVTVIAQESGDPAAVIFDAVSAARARGIDIVLADTAGRLP
                     TQLHLMDEIAKVRRVIQKVEPEGPHETLLVLDANIGQNALMQVKAFDKAVNVTGLVLT
                     KLDGTAKGGVVAAIARQCPKPIRFIGVGEQIDDLRPFSARDFVDALFDQ"
     gene            2062..>2523
                     /locus_tag="PLN02_09165"
     CDS             2062..>2523
                     /locus_tag="PLN02_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOI52533.1"
                     /translation="MTLRQLFPFLLAPWLLGQALANPYETTLDNGLRVIVKEDRRAPT
                     AVHMVWYRIGSMDEVDGQSGVAHALEHMMFKGTPSVGPGEFNRLVAAAGGRDNAFTSR
                     DYTAYFQQIPKEKLGEMMQLEADRMRHLNVDPKEFEQEIKVIMEERRMRTDD"
BASE COUNT          403 a          793 c          896 g          431 t
ORIGIN      
        1 ttccgccatg tcgccgtttt ccttgagccg acgcagggct tcgtcgcgcg gcacgaaggt
       61 gctgcgaacg ccgggctggg cacgcaggcg ctgttcgatc tcggcgactt cggccttgct
      121 ggcgccgact tccatgaaga cgctgacctg ctgcacggcg ctggcgtcgc ggccgggtcg
      181 cgcaggctgt cgagggcgac gtagccgaag gcgggcaggg tcagggcgat gccgatggcg
      241 aggagcgaca gcagggtgtt gagcggtgcc gcgaagaggc gacggagggc ccgcccgaaa
      301 gcggcgcgat gctgcgacag ccagcgtctc atacgactct cccgtgatcg aggcgcaagg
      361 cgttgtcgcc gcagtgttcg acccaggcct gatcgtgggt ggcgacgacg acggtgacgc
      421 cgacacgatg gaaatcggcg aacaggtcgc ggatgtcctg cgccgactcg cggtcgaggt
      481 tggcggtggg ttcgtcggcg agcagcacgg tcggccggtt gacgacggcg cgggcgatcg
      541 ccaggcgctg ctgttcgccg ccggagaggg cgatcggata ggctttttcg cgcgccagca
      601 ggccgacctt gtcgagcgcc gcctgggccc ggcgcaccgc ctcgcggtgc ggcaggccgg
      661 caatctgcag cggcagcagc acgttgtcga gggcgctgcg gtcgaagagc agtttctgat
      721 cctggaagat caggccgaag tggcggcgca cgtagggaat cgcgctacga cgcagtgccg
      781 acaggttctg cccgttgacc accaggctgc cggaggtcgg ccgttcgatc gaggcgatca
      841 gcttgaccag cgtcgtcttg ccggcgccgg agtggccggt gatcagcacc agttcgccgg
      901 cgcggatctc gaagctgacg ccgtgcagcg cttcgaagcc gcccgggtaa cgcttggtca
      961 gttggctggc gacgatcatt gatcgaacag ggcatccacg aaatcgcgcg ccgagaaggg
     1021 gcgcaggtcg tcgatctgct cgccgacgcc gatgaagcgg atcggtttcg ggcactgccg
     1081 ggcgatcgcc gcgacgacgc cgcccttggc ggtgccgtcg agcttggtca ggacgaggcc
     1141 ggtgacgttg accgccttgt cgaaggcctt cacttgcatc agcgcgttct ggccgatgtt
     1201 ggcatcgagc acgagcaggg tttcgtgcgg gccttccggt tcgaccttct ggatcacgcg
     1261 gcggaccttg gcgatctcgt ccatcaggtg cagttgcgtc ggcaggcggc cggcggtatc
     1321 ggccagcacg atgtcgatgc cgcgggcgcg ggccgccgac acggcgtcga agatgaccgc
     1381 cgccgggtcg cccgattcct gggcaatcac cgtcacgttg ttgcgctcgc cccaggtctg
     1441 cagctgttcg cgggcggcgg cgcggaaggt gtcgccggcg gcgagcagca cgctcttgcc
     1501 ctggttctgg aagtacttgg cgagcttgcc gatcgacgtc gtcttgccgg cgccattgac
     1561 cccggcgatc atgatcacgt agggtttgtg agtactcaca tcgagcggct gctcgagcgg
     1621 cgcgagggtt tcgtagaggg catcggcgag cgcccgctgg atcgcctcgg gcgtttccag
     1681 cttgtcgcgc ttggccgcct tcttcagttc gtcgagcagg tgggtggtcg cctcgatacc
     1741 gacgtcggaa gtcagcagca gggtttcgag ttcttcgagc agttcgtcgt cgaccttgcc
     1801 gcgcgcgaag atcgacttga gcttgccgcc ccactgggca cgggtccggg ccaggccctt
     1861 gaacaggcgt tcgcgccacg agggagcggc cgcgacctcg gccggcgcgg cggtcttggc
     1921 gtcgggcgcg gattttttca ggaaactgaa catgggtgtt aaggtctcag gccttgctga
     1981 caggcggaca gtcagccgtt aagatgccgt ccggatcgat gcagaaacag cgctgaattc
     2041 tagcagaaag cccccttccc catgaccctg cgacagcttt tccccttttt gctggccccc
     2101 tggctgctcg gccaggccct tgccaacccc tacgaaacga cgctcgacaa cgggctgcgc
     2161 gtgatcgtca aggaagaccg gcgggcgccg accgccgtgc acatggtctg gtaccggatc
     2221 ggcagcatgg acgaggtcga cggtcagtcc ggcgtcgcgc acgcgctcga gcacatgatg
     2281 ttcaagggca cgccgagcgt cggcccgggc gagttcaacc gcctggtggc ggccgccggc
     2341 gggcgcgaca acgccttcac cagccgcgac tacaccgcct acttccagca gattccgaag
     2401 gagaagctgg gcgaaatgat gcagctcgaa gccgaccgca tgcgccatct caacgtcgac
     2461 cccaaggaat tcgagcagga aatcaaggtc atcatggaag agcgccgcat gcgcaccgac
     2521 gac
//
LOCUS       DAOSDX010000401         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14477_length_2518_cov_2.883475, whole genome shotgun sequence.
ACCESSION   DAOSDX010000401 DAOSDX010000000
VERSION     DAOSDX010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14477_length_2518_cov_2.883475"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLN02_09170"
     CDS             complement(<1..274)
                     /locus_tag="PLN02_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI52534.1"
                     /translation="MRISEICIERPVFATVLSLVIMLLGIVSYDRLSVREYPKIDEPV
                     VTVSTDYRGASADIIESQVTKPLEDSLAGIEGVEVITSISRAERSQI"
     gene            complement(271..1368)
                     /locus_tag="PLN02_09175"
     CDS             complement(271..1368)
                     /locus_tag="PLN02_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI52535.1"
                     /translation="MKQMKRRGIVALSLAGLIGLGYFAWSANRAGQAVAAPEPAAKAA
                     PAGGPAKPVAVELAKVQAVDFSDETSAVGTLKAAESVVLRPEVSGRIARIGFRDGALV
                     RRGDLLIGLDATIQEAELAQAKANVELARTSFRRNEELLAKKFISPQALDASAATLKV
                     QEAALQLATAKAAKMRLVAPFAGIVGLRDVSVGGYVKEGEELVNVEDISSLRADFRLA
                     EAYFGRLRPGLALELSSDALPGQTFAATVEAIDPQIDPAARSIAVRARLDNRQGKLRP
                     GMFVRVRVAFGERKGVLMIPEEAVQPGAKPSVFRVVDGKAEVAPVRLGLRRDAQVEVV
                     EGLAAGDLIVTAGQLKLRPGAPVKMPQAAQP"
     gene            complement(1365..1904)
                     /locus_tag="PLN02_09180"
     CDS             complement(1365..1904)
                     /locus_tag="PLN02_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOI52536.1"
                     /translation="MTKRRPWWSERLRQAALLGAGLGLAGAALALELPAVSGNSIVDG
                     LASFYSNRFHGRKTATGERFDKKQLTAASNHFPLGSHVAVLRPASGLCVIVRINDRMH
                     HRQRGGRVIDLSAAAADYLEMLKEGIVQVRLAHLPDNWRERGPQACGEAFTGGEAPRG
                     EPGLLEQIENGISQGITQP"
     gene            complement(1876..>2518)
                     /gene="purM"
                     /locus_tag="PLN02_09185"
     CDS             complement(1876..>2518)
                     /gene="purM"
                     /locus_tag="PLN02_09185"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOI52537.1"
                     /translation="GCALIGGETAEMPGMYPAGEYDLAGFAVGVAEKSKLIDGKSICE
                     GDVVLGLASSGAHSNGYSLVRKIIERSNPDMDAPFDGERSLAEVVMAPTRIYVKQVLA
                     TMQKVTIKGMAHITGGGLLENVPRVLPENTVAELQKAAWPRPKLFDWMQQEGQVDEKE
                     MHRTFNCGIGMVIIVSAAEADAAEAELKAQGEAVYRIGRIRARQGDEAQTLVV"
BASE COUNT          380 a          877 c          840 g          421 t
ORIGIN      
        1 aaatctggct gcgctcggcg cgcgaaatcg aggtgatgac ctcgacgcct tcgatgccgg
       61 ccagcgaatc ttccagcggc ttggtcacct gcgactcgat gatgtcggcg gaggcgccgc
      121 ggtaatcggt cgacacggtg accaccggct cgtcgatctt cgggtattcg cgcaccgaca
      181 gccggtcgta ggaaacgatg ccgagcagca tgatgacgag cgacagcacg gtcgcgaaga
      241 ccggccgctc gatgcagatc tcggaaattc tcatggttgt gccgcttgcg gcatcttcac
      301 cggagcgccg gggcgcagct tcaactggcc agcggtgacg atcaggtcgc cggcggccag
      361 cccctcgacc acctcgacct gcgcgtcgcg gcgcaggccg agcctgaccg gcgccacttc
      421 cgccttgcca tcgacgacgc gaaacaccga cggcttggcg cccggttgca ccgcttcctc
      481 gggaatcatc agcacgccct tgcgttcgcc gaaggcgacg cggacgcgca cgaacatgcc
      541 cgggcgcaac ttgccctggc ggttgtcgag gcgggcgcgg acggcgatcg agcgcgccgc
      601 cggatcgatc tgcgggtcga tcgcctcgac cgtcgccgcg aaggtctggc cgggcagcgc
      661 gtcgctggaa agttcgagcg ccagccccgg ccgcaggcgg ccgaaatagg cttcggcgag
      721 gcggaagtcg gcgcgcaggc tggagatgtc ctcgacgttg accagttcct cgccttcctt
      781 gacgtagccg ccaacgctca cgtcgcgcag cccgacgatg ccggcaaacg gcgccaccag
      841 gcgcatcttg gcggccttgg cggtagccag ctggagcgcc gcttcctgca ccttgagcgt
      901 cgccgccgag gcatcgagcg cctgcgggct gatgaacttc ttggccagca gttcctcgtt
      961 gcgccggaaa cttgtccgcg ccagctcgac gttggccttg gcttgcgcca gctcggcttc
     1021 ctggatcgtc gcgtcgaggc cgatcagcag gtcaccgcgc cgcaccaggg caccgtcgcg
     1081 aaagccgatg cgggcgatac ggccgctgac ttcgggacgc agcaccaccg attcggccgc
     1141 cttcagcgtg ccgaccgcgc tcgtctcgtc gctgaaatcg accgcctgca ccttggccag
     1201 ttcgaccgcc accggctttg ccgggccgcc ggccggcgcc gccttggccg ccggttccgg
     1261 cgcggcaacg gcctggccgg cgcggttggc cgaccaggca aagtagccga gcccgatcag
     1321 gccggccagg gaaagcgcga caatgccgcg ccgcttcatc tgcttcatgg ctgggtgatt
     1381 ccttgggaaa tgccgttttc gatctgttcc agcaagccgg gttcgccgcg cggcgcctcg
     1441 ccgccggtga aggcttcgcc gcaggcctgc ggaccgcgct cgcgccagtt atcgggcagg
     1501 tgcgccagac gcacctggac gatgccttcc ttcaacattt cgaggtaatc ggcggcggcc
     1561 gccgacaggt cgatgacccg tccgccgcgt tgccggtggt gcatgcggtc gttgatgcgg
     1621 acgatgacgc acagcccact cgctggtcgc agcacggcga cgtggctgcc gagcggaaaa
     1681 tggttgctgg cggcggtgag ctgcttcttg tcgaaccgtt cgccggtggc ggtcttgcga
     1741 ccgtggaaac ggttgctgta gaaactggcc agcccgtcga cgatcgagtt gccgctcacc
     1801 gccggcaact cgagcgccag cgccgccccc gccagaccga ggccggcgcc gagcagcgcc
     1861 gcctggcgca ggcgctcaga ccaccagggt ctgcgcttcg tcaccctgcc gtgcccgaat
     1921 ccggccgatg cggtaaaccg cctcgccctg cgccttcagt tcggcctcgg cggcatccgc
     1981 ctcggcggcc gagacgatga tgaccatgcc gatgccgcag ttgaaggtgc ggtgcatttc
     2041 cttctcgtcg acctggcctt cctgctgcat ccagtcgaac agcttgggac gcggccaggc
     2101 ggccttctgc agctcggcga cggtgttttc cggcagcacg cgcggcacgt tctcgagcag
     2161 gccgccgccg gtgatgtggg ccatgccctt gatcgtcacc ttctgcatcg tcgccagcac
     2221 ctgcttgacg tagatgcggg tcggcgccat gacgacctcg gcgaggctac gctcaccgtc
     2281 gaacggcgca tccatgtccg ggttggaacg ttcgatgatc ttgcgcacca gcgaatagcc
     2341 gttggaatgc gcgccggagg aagcgaggcc gagcaccacg tcgccttcgc agatcgactt
     2401 gccgtcgatc agcttcgact tctcggcgac gccgacggca aagccggcca ggtcgtattc
     2461 gccggcaggg tacatgcccg gcatttcggc ggtttcgccg ccgatcagcg cgcagccg
//
LOCUS       DAOSDX010000402         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14511_length_2514_cov_3.379829, whole genome shotgun sequence.
ACCESSION   DAOSDX010000402 DAOSDX010000000
VERSION     DAOSDX010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14511_length_2514_cov_3.379829"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLN02_09190"
     CDS             <1..1103
                     /locus_tag="PLN02_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOI52538.1"
                     /translation="RLLDSPWLQQAWAGIDPAGLWDCHVHLAGLGDGGSGIEVGPQLS
                     SPLHPLHYAQRLFYMNAGCAHDAPGAVDAAYTARLLNLCEAMPEGFKVMLFAFERFHD
                     AAGQAHPEHSAFYVPNAWAAKLAREFPARFEWVASLHPYHATAVDDLGQAIAGGARAV
                     KWLPAAQGMDPADAKCDAFYRVLAESGTPLIIHCGEEQAVRGSDTQAFGNPLRLRRAL
                     DLGVKVVVAHCATMGSDLDDDGAVRPSFDLFLKAMAEPRAKDLLYGDISAIALRNRQP
                     AVIRTLLERGDLHERLLHGSDYPLPGILPLISAATLARAGLLPKAAVADLDALREHNP
                     LLFDFVLKRLLNWQGHSFSPAVFNTRRIFSGQ"
     gene            1106..>2514
                     /locus_tag="PLN02_09195"
     CDS             1106..>2514
                     /locus_tag="PLN02_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI52539.1"
                     /translation="MSGQFGLLKEKRFGPLFGTQFLGAFNDNLYKNALVVLLTFQAAQ
                     WTTIAPELLGNLAAGIFILPFFLFSATAGQLADKYDKATLARLSKLLEILIMGIAAVG
                     FALHSLVWLLAALFLLGLQSTQFGPVKYAILPQHLHEDELVGGNALIEAGTFVSILIG
                     TLAGGLLAGSVAQPMWIAVGGLAVAVLGYLYSRGIPTAPPPAPELRINWNPLSETWRS
                     INFARGNRSVFLSILGASWFWLYGALFLAQFPAYTKNVLGGGETMVTLLLAIFTVGIG
                     VGSLLCERMSGKHVEIGLVPFGSIGLTLFGIDLYFASPVGLVGTTAHELTALLSLPAV
                     WRVLFDLLMLGMFGGFFIVPLYALVQSRSEPAHRARIIAANNILNALFMVVGALGAAA
                     ALGAGLSIPALFGIAALLNAAVAVYIYGLVPEFLLRFVAWLLVKAVYRLRSEGVEHIP
                     LDGPALLVANHVSFADAVIL"
BASE COUNT          347 a          891 c          815 g          461 t
ORIGIN      
        1 tgcgactgct cgacagcccc tggctgcagc aggcctgggc cggtatcgac ccggccgggc
       61 tctgggattg ccacgtccat ctcgccggac tgggcgatgg cggcagcggc atcgaagtcg
      121 ggccgcagtt gtcgtcgccg ctacacccgc tgcattacgc gcagcgcctg ttctacatga
      181 acgccggttg cgcccacgat gcgccgggtg ccgtcgatgc cgcctacacg gcgcgcctgc
      241 tcaacctgtg cgaggcgatg ccggaaggct tcaaggtcat gcttttcgcc ttcgagcgtt
      301 tccacgacgc ggccggccag gcgcacccgg aacattcggc cttctacgtg ccgaacgcct
      361 gggcggcgaa gctggcgcgc gagttcccgg cgcgcttcga gtgggtggcg tcgctgcatc
      421 cctaccacgc gacggcggtc gacgacctgg gccaggcgat tgccggcggc gcccgcgccg
      481 tcaaatggct gccggcggcg cagggcatgg acccggccga cgcgaagtgc gatgccttct
      541 accgcgtgct ggccgagtcc ggcacgccgc tgatcatcca ttgcggcgag gagcaggcgg
      601 tgcggggcag cgacacgcag gccttcggca acccgctgcg cctgcgccgg gcgctcgacc
      661 tcggcgtcaa ggtcgtggtc gcccattgcg caacgatggg cagcgacctc gacgacgacg
      721 gcgccgtgcg ccccagtttc gacctcttcc tcaaggcgat ggccgagccg cgcgccaagg
      781 acttgctcta cggcgacatc tcggcgatcg cgctgcgcaa tcgccagccg gcggtgatcc
      841 gcaccctgct cgaacgcggc gatctgcacg agcgactgct gcacggttcc gactacccgc
      901 tgcccggcat cctgccgctg atttcggcgg cgacgctggc ccgcgccggg ctgctgccga
      961 aagccgccgt cgccgatctc gacgcgctgc gcgagcacaa tccgctactc ttcgatttcg
     1021 tcctcaagcg cctgctcaac tggcagggac attccttctc gccggcggtc ttcaataccc
     1081 gccgcatctt ttcgggccag tagtcatgag cggacaattc ggactcctca aggaaaaacg
     1141 tttcgggcca ctgttcggca cccaattcct cggcgccttc aacgacaacc tgtacaagaa
     1201 cgcgctggtc gtgctgctga ccttccaggc ggcgcaatgg acgacgatcg cgccggaact
     1261 gctgggcaac ctggcggccg gcatcttcat cctgcctttc ttcctctttt cggcgaccgc
     1321 cgggcaactc gccgacaagt acgacaaggc gacgctggcg cggctttcca agctgctgga
     1381 aatcctcatc atgggcatcg ccgccgtcgg tttcgcgctg cacagcctgg tctggctgct
     1441 cgccgcgttg ttcctgctcg gcctgcaatc gacgcagttc gggccggtca aatacgcgat
     1501 cctgccgcag cacctgcacg aagatgaact ggttggtggc aacgcgctga tcgaggccgg
     1561 taccttcgtc tcgatcctga tcggcaccct ggccggcggc ctgctcgccg gcagcgtcgc
     1621 gcagccgatg tggatcgccg tcggcgggct ggcggtcgcc gtgctcggtt acctctacag
     1681 ccgcggcatc ccgacggcgc cgccaccggc gccggaactg cggatcaact ggaacccgct
     1741 gagcgagacc tggcgcagca tcaatttcgc ccgcggcaac cgcagcgtct tcctctccat
     1801 cctcggtgcg tcctggttct ggctgtacgg cgcgctgttc cttgcccagt tcccggccta
     1861 cacgaagaac gtgctgggcg gcggcgaaac gatggtgacg ctgctgctcg ccatcttcac
     1921 cgtcggcatc ggcgtcggct cgctgctttg cgagcgaatg tccggcaagc acgtcgagat
     1981 cggcctggtg cccttcggct cgatcggcct gacgctgttc ggcatcgacc tctacttcgc
     2041 gtcgccggtt ggcctggtcg gcacgacggc acacgagctg accgccttgc tcagtctgcc
     2101 ggcggtctgg cgcgtgctgt tcgacctcct gatgttgggg atgttcggcg gcttcttcat
     2161 cgtcccgctc tacgcgctgg tgcagtcgcg cagcgagccg gcgcaccgcg cccggatcat
     2221 cgccgccaac aacatcctga atgcgttgtt catggtcgtt ggcgcgctcg gcgcggcggc
     2281 ggcgctcggc gccgggctgt cgatcccggc gctgttcggc atcgcggcgc tgctcaacgc
     2341 ggcggtcgcg gtctacatct acgggctggt gccggaattc ctgctccgct tcgtcgcctg
     2401 gctgctggtc aaggcggtgt accggctgcg cagcgagggc gtcgagcaca ttccgctgga
     2461 cgggccggcg ctgctcgtcg ccaaccacgt cagcttcgcc gacgcggtga tcct
//
LOCUS       DAOSDX010000403         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14512_length_2514_cov_2.873119, whole genome shotgun sequence.
ACCESSION   DAOSDX010000403 DAOSDX010000000
VERSION     DAOSDX010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14512_length_2514_cov_2.873119"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /gene="trkA"
                     /locus_tag="PLN02_09200"
     CDS             complement(<1..1338)
                     /gene="trkA"
                     /locus_tag="PLN02_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOI52540.1"
                     /translation="MKIVILGAGQVGASVAEGLVSEENDITIVDQDPQRLAQLQDRLD
                     LRTVVGDAATPSVLRSAGAEDADMVIAVTQSDQSNLVACKLAHSVFNIPTRIARLRSR
                     DFLEDEVLLSDDNFAVDFALCPEQVITDYIRRLIEFPRALQVLNFARGRVCLVGVRAY
                     DGGLIVGKPIKAMRDLLPPEIDARIAAIFRDNQPVFPEGDTIVKSGDEVFLLAATENI
                     HPVLNQLRREVSRVERIMIAGGGNIGLRLAKSLEGHYEVKLIEGRRPRAELLANELND
                     TLVLCGDATDEELLEQENIGEMDLFLAMTNDDEDNIMAGSLAKRLGCKRVVALINRRA
                     YAEMIEGGPIDIGISPAQVSIGTLLAYVRQGDVAEVHSLRRGAAEALEIVAHGDRNTS
                     KVVGRRIDEVEWPRGVTVAAMVRNFEEPMVVGQTDDWTAITRYGEVVIAHHDTV"
     gene            complement(1355..>2514)
                     /locus_tag="PLN02_09205"
     CDS             complement(1355..>2514)
                     /locus_tag="PLN02_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52541.1"
                     /translation="ARRVRSELRPDLVLLDIWMPDTDGISLLKEWHAGGHLNMPVVMM
                     SGHGTIDTAVEATRLGACDFLEKPIALQKLLGSVQKALKHDAPPTRPPLTLEAFSRLP
                     FVKEFRRRLEQAAAKASVVLIKGATAGMAEIAARTLLAPRAPWLDLSTTSSALSQEVL
                     EKTSGGILFVPDLAALGKMQQMNLAFAVDRADKLNLQLIAASTTPLSVLAEHGWDSKL
                     IARLGEIWVALPALAGHVDDLPEIASLLLGNFVERGEVPHRRFSTAALNALRTLPWKR
                     HADASWNVLYGLVRNLALTALDDEIDADDVARVMPEQEEESGAALSFLPLFEQPLREA
                     RDAFEKLYFEHHLRQEGGNMTRLAERSGLERTHLYRKLKQLDIRLGKRNDE"
BASE COUNT          416 a          805 c          846 g          447 t
ORIGIN      
        1 caccgtgtcg tggtgggcga tcaccacctc gccgtagcgg gtgatcgccg tccagtcatc
       61 ggtctggccg acaaccatcg gttcttcgaa attgcgcacc atggcggcaa cggtgacgcc
      121 gcgtggccac tcgacttcat cgatgcggcg gccgaccacc ttggaggtat tgcggtcgcc
      181 gtgggcaacg atctccagcg cttcggcggc gccacgacgc agcgaatgaa cttcggcgac
      241 gtcgccctgg cggacgtaag cgagcagcgt gccgatcgag acctgggccg gcgagatgcc
      301 gatgtcgatc ggcccgccct cgatcatctc ggcgtaggca cggcggttga tcagggcgac
      361 gacgcgcttg cagccaaggc gcttggccag gctgccggcc atgatgttgt cttcgtcgtc
      421 gttggtcatg gcgaggaaga gatccatctc gccgatgttt tcctgctcca gcaattcctc
      481 gtcggtggcg tcgccgcaca gcaccagggt gtcgttgagc tcgttggcaa gcagttcggc
      541 gcgcggccgc cggccttcga tcagcttgac ctcgtaatgc ccttccagcg acttggccag
      601 gcgcaggccg atgttgccgc caccggcgat catgatccgc tcgacccggc tgacttcgcg
      661 ccgcagctgg ttgaggaccg gatggatgtt ctcggtggca gcgagcagga agacctcgtc
      721 gccactcttg acgatggtgt cgccctcagg gaaaaccggc tggttgtcgc ggaagatggc
      781 ggcgatgcgg gcatcgattt ccggcggcag caggtcgcgc atggccttga tcggcttgcc
      841 gacgatcagc ccgccgtcgt aggcgcggac gccgaccaga cagacccggc cgcgggcaaa
      901 gttgagcacc tgcagggcgc gcggaaattc gatcaggcga cgaatgtagt cggtgatcac
      961 ctgttccggg cagagcgcga aatcgactgc gaagttgtcg tccgagagca gcacctcgtc
     1021 ttcgagaaag tcgcgcgaac gcaggcgggc gatgcgggtc gggatgttga agacggaatg
     1081 cgccagcttg caggcgacca ggttggactg gtcgctctgg gtcacggcga tcaccatgtc
     1141 ggcgtcctcg gccccggcac tgcgcagcac cgacggtgtc gccgcgtcac cgacgacggt
     1201 gcgcaggtcg aggcggtcct gcaactgggc gagacgctgc gggtcctggt cgacgatggt
     1261 gatgtcgttc tcttccgaga ccaggccttc ggcaacgctg gcgccgacct ggccggcgcc
     1321 gagaatgaca atcttcatgc cccctccccc gtcttcattc gtcgttgcgt ttgccgagcc
     1381 ggatgtcgag ctgcttcagc ttacggtaga ggtgggtgcg ttcgagcccg gaacgctccg
     1441 ccagccgggt catgttgccg ccttcctggc gcaggtgatg ttcgaagtag agtttctcga
     1501 aggcatcgcg cgcctcgcgc agcggctgct cgaagagcgg caggaaggaa agcgcggcgc
     1561 cgctctcctc ctcctgctcg ggcatcaccc gggccacgtc gtcagcgtcg atctcgtcgt
     1621 cgagcgcggt cagcgccaga ttgcgcacca acccgtagag aacgttccag ctggcatcgg
     1681 catggcgttt ccagggcaac gtgcgcaagg cgttgagcgc cgccgtcgag aaacgccgat
     1741 gcggtacttc gccgcgttcg acgaaattgc cgagcagcag gctggcgatt tccggcagat
     1801 cgtcgacatg accagcgagc gccggcaagg ccacccagat ctcgccgagg cgggcgatca
     1861 gcttgctgtc ccagccatgc tcggcaagca cggaaagcgg cgtggtactg gcggcgatca
     1921 gttgcaggtt aagcttgtcg gcgcggtcga cggcaaaggc caggttcatc tgctgcatct
     1981 tgcccaacgc ggcaagatcg ggaacgaaaa ggatgccgcc gctggtcttt tccagcactt
     2041 cctggctcag cgcactgctc gtcgtcgaca ggtcgagcca gggcgcgcgt ggcgccagca
     2101 gcgtacgcgc ggcgatctcc gccatgccgg cggtggcgcc cttgatcagc accaccgagg
     2161 ccttggccgc cgcctgctcc agccggcggc ggaattcctt gacgaagggc aggcgggaaa
     2221 aagcttccag cgtcagcggc gggcgcgtcg gcggcgcgtc gtgcttgagc gccttttgca
     2281 cgctgccgag cagtttctgc agggcaatcg gcttttccag gaagtcgcag gcacctaagc
     2341 gcgtcgcctc taccgcggta tcgatcgtcc cgtgaccgga catcatcacc accggcatgt
     2401 tgaggtgacc tccggcatgc cactccttga gcagcgaaat gccgtcggta tccggcatcc
     2461 agatgtcgag cagcaccagg tccgggcgta gctcgctacg cacgcggcgc gccg
//
LOCUS       DAOSDX010000404         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14564_length_2507_cov_2.915579, whole genome shotgun sequence.
ACCESSION   DAOSDX010000404 DAOSDX010000000
VERSION     DAOSDX010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14564_length_2507_cov_2.915579"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLN02_09210"
     CDS             complement(<1..482)
                     /locus_tag="PLN02_09210"
                     /inference="COORDINATES: protein motif:HMM:NF033682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retention module-containing protein"
                     /protein_id="HOI52542.1"
                     /translation="MSAAISIAKAASVTGQVFAISPDGKVRPLHPGDPLFPGEKVVTA
                     RDAQVVVKLADGREISLERSESLQLDGDVAGNSSQDDSAVAAGSPGVRELTAALAANR
                     PLDDLLDEEATAAGESGAASEGGHNFVQFQRIVESIGSSDSAYRFGAGDRYALAERQN
                     G"
     regulatory      722..801
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            896..1135
                     /locus_tag="PLN02_09215"
     CDS             896..1135
                     /locus_tag="PLN02_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52543.1"
                     /translation="MIKNHQAFAPTPLHGLYRRRTTACAPAPASVWLSRYLPGLILKL
                     ALLILVCQWVPLYGGLIEDLFGPLTQSLSFALTRL"
     gene            1190..>2507
                     /locus_tag="PLN02_09220"
     CDS             1190..>2507
                     /locus_tag="PLN02_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52544.1"
                     /translation="MPFRNLLLDDEKACVLAVDDTPAILQLLEMALGIDYEVVTAESG
                     AQALEIFPQRRFDLVLLDLLMPGMNGFETLAELQKLPSFNGTPVIFLTAMDDLASECS
                     ALEMGADDYITKPFKPNLVRLRIANILHRVRLQRHLSLALAGADQGLWSWDLASDQVR
                     VAAGWSEPLGCANGEAAGEVAGWAALAHADCGAVIEQARAAYLAGERPAFEADVRLRT
                     LKGGWKWFNIYGKSGNRQSLVGTYRNIDAKKAAELALRDSEERLRHVMDATGEGIWDW
                     QVGSAMARHNAAWSRILGLDEGAHEHEIACVAALIHPDDRPAHAAALAACLERAPSFA
                     LEYRIRHASGNYLWVMDRGKVVERAADGTALRLVGAFQDISESKRLEEEYRRLALFDT
                     LTGLPNRRLLVDRMEQAIVQNRRQASLGALMFIDMDRFKELNDSLGH"
BASE COUNT          431 a          770 c          827 g          479 t
ORIGIN      
        1 ccgttctggc gctcggccag ggcgtaacgg tcgccggcgc cgaaacggta ggcggagtcg
       61 ctgctgccga tgctttcgac gatgcgctgg aactgcacga agttgtggcc gccttcgctg
      121 gcggcgccgc tttcaccggc ggcggtggct tcctcgtcga gcaggtcgtc gagcggccgg
      181 ttggcggcga gcgccgccgt cagttcgcgg acgccggggc tgcccgccgc cactgcgctg
      241 tcgtcctggc tgctgttgcc ggcgacatcg ccgtcgagtt gcaggctttc gctgcgctcc
      301 aggctgattt cccggccgtc ggcaagtttg acgacgactt gcgcgtcgcg cgcggtgacc
      361 accttctcgc cggggaagag cgggtcgccg gggtgcaagg gacgaacctt gccgtcgggc
      421 gagatggcga aaacctggcc ggtgacggaa gctgctttgg cgatgctgat ggcggctgac
      481 atggcttgaa ctccttgcgc gctggggctt gctctgacga ggaaagaagt tgcagtcaga
      541 gttaattgaa agtcgtgttg tcaaattata cgtatacatc aattaaatgc tatacgtgaa
      601 aaccaagttt cattttattc gattggcaag cagcggaatg ctggatgttg tttttgtgat
      661 tattcagcga ataaacgata aaaatagttg acgtgcctgt atttgcgatc tagcatgcag
      721 tcgtgatcaa cggcaaacct gcggcgacgc agggacgcaa agcctccggt ctggcgcttg
      781 tcagatagcg gggttgccca gcccaagatc tcggccatct gccgggacct gcccgaaaac
      841 tccaacgggc gctcgcgtga tggtctttcc aacgtatcaa gacaaaggaa agatcatgat
      901 caagaaccac caggctttcg ctcccactcc cctgcacggt ctttatcgcc gtcggacgac
      961 ggcttgcgcg cccgcaccgg ccagcgtctg gctgagtcgc tacctgccgg gactgatcct
     1021 gaaacttgcg ctgctgattc tggtctgtca gtgggtgccg ctatacggcg gtctaattga
     1081 agacctgttc gggccgctca cgcagagctt gagttttgcc ctgactcgcc tttagtatga
     1141 cgtatcagtt ttgcggcgtt aattcatttt taccggtggc ggagcgagcg tgcctttcag
     1201 aaacctcttg cttgatgacg agaaggcctg cgtgctggcg gtcgatgaca cgccagccat
     1261 cctgcaattg ctggagatgg cgctcggcat cgattacgaa gtggtgaccg ccgaatccgg
     1321 ggcgcaggcg ctggaaatct tcccgcagcg gcgcttcgac ctcgtgctgc tcgatctgct
     1381 gatgccgggg atgaacggtt tcgagaccct ggccgagttg cagaagttgc cgtcgttcaa
     1441 cggtacgccg gtgatcttcc tgaccgccat ggacgacctg gcgagcgagt gcagcgcgct
     1501 ggaaatgggc gccgacgact acatcaccaa gccgttcaag cccaacctgg tgcgcctgcg
     1561 catcgccaac atcctgcatc gcgtccgcct gcagcgtcat ctctcgctgg ccctggccgg
     1621 cgccgaccag ggcctgtggt cctgggatct ggcgagcgac caggtgcgcg tcgccgccgg
     1681 ctggagcgag ccgctgggct gcgccaacgg cgaggcggcg ggcgaggtcg ccggctgggc
     1741 ggcactggcg cacgccgatt gcggcgccgt catcgagcag gcgcgggcgg cctacctggc
     1801 cggcgagcgt ccggccttcg aggccgacgt ccggctgcgc acgctgaagg gcggctggaa
     1861 gtggttcaac atctatggca agtcgggcaa ccggcagtcg ctggtcggca cctaccgcaa
     1921 catcgacgcc aagaaggcgg ccgaactggc gctgcgcgac agcgaggagc gcttgcgcca
     1981 cgtcatggac gctaccggcg aaggcatctg ggattggcag gtggggagcg ccatggcccg
     2041 ccacaacgcc gcctggagcc gcatcctggg cctcgacgaa ggcgcgcacg agcacgagat
     2101 cgcttgcgtc gctgccttga tccatccaga cgaccggccg gcgcacgctg ccgcgctcgc
     2161 tgcctgcctg gagcgggcgc cgtccttcgc cctcgaatac cggattcgcc acgccagcgg
     2221 caattatctg tgggtcatgg atcgcggcaa ggtggtcgag cgcgcggcag atggcacggc
     2281 actgcgcctg gtcggcgcct tccaggacat ttccgagagc aagcggctgg aggaggaata
     2341 ccggcggctg gccttgttcg ataccctgac cggcttgccc aaccgccgcc tgctggtcga
     2401 ccgcatggaa caggcgatcg tccagaaccg ccgccaggcg tcgctcggcg cgctgatgtt
     2461 catcgatatg gaccgcttca aggaactcaa cgacagcctc ggccatg
//
LOCUS       DAOSDX010000405         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14587_length_2503_cov_3.005310, whole genome shotgun sequence.
ACCESSION   DAOSDX010000405 DAOSDX010000000
VERSION     DAOSDX010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14587_length_2503_cov_3.005310"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLN02_09225"
     CDS             <1..309
                     /locus_tag="PLN02_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52545.1"
                     /translation="AYRAAIVADSDFLPGGSAGGANPCLDCATRPCLAACPAGAAGEP
                     FLLTACSDERLRPGSPCARSCLARLACPVGSAHRYDDAQLSHSYGQSLAMLESWRRQR
                     "
     gene            complement(323..727)
                     /locus_tag="PLN02_09230"
     CDS             complement(323..727)
                     /locus_tag="PLN02_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52546.1"
                     /translation="MNPPIESGPGGVRPSLVNVTHLIYGLHAIAVLIGVTSSATIVGG
                     FVFGLPSLLGVFLNYWMRSEVRGTWLESHFRWQIRTFWFTVLWLGVYGLFVITIIGIP
                     IAFLLILGLGFWVAYRVIRGWLALNAGETINA"
     gene            complement(724..1221)
                     /locus_tag="PLN02_09235"
     CDS             complement(724..1221)
                     /locus_tag="PLN02_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI52547.1"
                     /translation="MSLRVIDLDLNDAAHAAALIDLLDHYAQDPMGGGNGLKPAVKAT
                     LVDGLKSVSFFHGGLALAGDEAVGLINCFAGFSTFAAKPLLNIHDIVVRRERRRQGIG
                     QALLAWAEARARALGCCKLTLEVLSNNHQAMASYQLAGFAPYVLDPAAGQALFLQKML
                     SEEKT"
     gene            complement(1218..>2503)
                     /gene="ubiD"
                     /locus_tag="PLN02_09240"
     CDS             complement(1218..>2503)
                     /gene="ubiD"
                     /locus_tag="PLN02_09240"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HOI52548.1"
                     /translation="TNLFGTPKRVALGMGEESVQALREVGKLLAYLKEPEPPKGLKDA
                     WDKLPVLKQVLNMAPKKISNAPCQEVVWEGKDVDLSRLPIQHCWPGDVAPLITWGLVV
                     TKGPHKNRQNLGIYRQQVLGPNKVIMRWLAHRGGALDFRDHQQANPGQPFPLAVVLGC
                     DPATILGAVTPVPDSLSEYQFAGLLRGGKTELTQCLGPGLQVPASAEIVLEGHIHPGE
                     KALEGPYGDHTGYYNEQAEFPVFTIDRITMRRNPIYHSTYTGKPPDEPAVLGVALNEV
                     FVPLLQKQYPEIVDFYLPPEGCSYRMAIVSIKKAYPGHAKRIMFGIWSFLRQFMYTKT
                     IVVVDEDIDIRDWKEVVWAMTTRFDAVRDTTLVDNTPIDYLDFASPVAGLGSKMGIDA
                     TNKWPGETQREWGRPIVMDTDVKQRIDNLWGSLGL"
BASE COUNT          414 a          828 c          838 g          423 t
ORIGIN      
        1 gcctaccgtg ccgccatcgt cgccgacagc gatttcctgc ccggcggcag tgccggtggc
       61 gccaatccct gcctcgactg cgctactcgc ccctgcctgg ccgcctgccc ggccggcgcc
      121 gccggcgagc ctttcctgct caccgcctgc agcgacgaac gcctgcgccc cggctcgccc
      181 tgcgcccgca gctgcctggc gcgcctggcc tgcccggtgg gcagcgcaca tcgctacgac
      241 gacgcccaac tcagccacag ctacgggcag tcgctggcga tgctggaaag ctggcggcgg
      301 cagcgctgag cgcgcgccgg aatcaggcgt tgatcgtctc gccggcgttg agcgccagcc
      361 agccgcgaat gacgcggtag gcgacccaga aaccgaggcc gaggatgagc aggaaggcga
      421 tcgggatgcc gatgatggtg atcacgaaca ggccgtagac gccgagccac agcacggtga
      481 accagaaggt ccggatctgc cagcggaaat ggctctccag ccaggtgccg cggacttcgc
      541 tgcgcatcca gtaattgagg aagacgccga gcaaggacgg cagcccgaag acgaaaccgc
      601 cgacgatggt cgccgacgag gtgaccccga tcagtacggc tatcgcgtgc aggccgtaga
      661 tcaggtgggt gacattgacc agcgacggac ggacgccgcc ggggccggat tcgatcggtg
      721 ggttcatgtc ttctcctcgc tcagcatctt ctgcaggaac agcgcctggc cggccgccgg
      781 gtcgagcacg tagggcgcga aaccggcgag ctggtaggaa gccatcgcct ggtggttgtt
      841 cgacagcact tccagcgtca gcttgcagca gccgagcgcg cgcgccctgg cctcggccca
      901 ggccagcagc gcctgaccga tgccctggcg gcggcgctcg cggcggacga cgatgtcgtg
      961 aatgttgagc aacggcttgg cggcgaaggt cgagaagccg gcgaaacagt tgatcaggcc
     1021 gaccgcttcg tcaccggcca gcgccaggcc gccgtggaag aaagacacgc tcttcaggcc
     1081 atcgaccagc gtcgccttca ctgccggctt gaggccgttg ccgccgccca tcgggtcctg
     1141 cgcatagtgg tcgagcaggt cgatgagcgc tgccgcatgt gcggcgtcgt tgaggtcgag
     1201 atcgatgacg cgcaggctca taggccgagg ctcccccaga ggttgtcgat gcgttgcttg
     1261 acgtcggtgt ccatgacaat cggccgcccc cattcgcgct gggtttcgcc cggccacttg
     1321 ttggtcgcgt cgatccccat cttcgagccg aggccggcga ccgggctggc gaagtcgagg
     1381 tagtcgatcg gcgtgttgtc gaccagggtc gtgtcgcgca ccgcgtcgaa gcgcgtggtc
     1441 atcgcccaga ccacttcctt ccagtcgcgg atgtcgatgt cctcgtccac cacgacgatc
     1501 gtcttggtgt acatgaactg gcgcaggaag ctccagatgc cgaacatgat gcgcttggcg
     1561 tggccgggat aagccttctt gatcgagacg atagccatcc ggtaggaaca gccttccggc
     1621 ggcaggtaga aatcgacgat ctccggatac tgcttctgca gcagcggcac gaacacctcg
     1681 ttgagcgcga cgcccaacac cgccggttcg tcgggcggct tgccggtgta ggtgctgtgg
     1741 tagatcgggt tccggcgcat ggtgatgcgg tcgatggtga acaccgggaa ttccgcctgc
     1801 tcgttgtagt agccggtatg gtcgccatag ggcccttcga gcgccttctc gccgggatga
     1861 atatggcctt ccagaacgat ctcggcggaa gcgggcactt gcaaaccggg gcccaggcac
     1921 tgcgtcagct cggtcttgcc gccgcgcaac aggccggcga actggtattc ggaaagactg
     1981 tccggcaccg gcgtcaccgc gccgaggatg gtcgccggat cgcagccgag caccaccgcc
     2041 aacgggaaag gctggcccgg gttggcctgc tgatggtcgc ggaagtcgag cgcgccgccg
     2101 cggtgcgcca gccagcgcat gatcaccttg ttcggcccga gcacctgctg gcggtagatg
     2161 ccgaggttct gccggttctt gtgcggcccc ttggtgacga ccaggcccca ggtgatcaac
     2221 ggcgccacgt cgcccggcca gcaatgctgg atcggcaggc gcgacaaatc gacgtccttg
     2281 ccctcccaaa ccacttcctg gcagggcgcg ttgctgatct tcttgggcgc catgttgagc
     2341 acttgcttga gcaccggcag tttgtcccaa gcgtccttca ggcctttcgg cggttccggc
     2401 tccttcaggt aggcgagcag cttgccgacc tcgcgcagcg cctgcaccga ttcctcgccc
     2461 atgcccagcg ccacgcgctt cggcgtgccg aacaggttgg tga
//
LOCUS       DAOSDX010000406         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14614_length_2500_cov_3.055215, whole genome shotgun sequence.
ACCESSION   DAOSDX010000406 DAOSDX010000000
VERSION     DAOSDX010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14614_length_2500_cov_3.055215"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLN02_09245"
     CDS             <1..594
                     /locus_tag="PLN02_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOI52549.1"
                     /translation="TWEVDRDQLFKNNEVHDISVAVGVDPHGIEKIDEVDLSGLRYGR
                     IIVMSDADVDGSHIQVLLFTLFLKHFPALVEKGHIHVAQPPLFRVDVEAKGHTRKIYC
                     LDEAEKEQTLRKLRDEGVNENKITVSRFKGLGEMNPDQLWETTLCPDTRRLVPFQAER
                     ETIRRMHQTFTLLMGKGEASGRRQWMEKDGGLVETDV"
     gene            complement(628..1086)
                     /locus_tag="PLN02_09250"
     CDS             complement(628..1086)
                     /locus_tag="PLN02_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52550.1"
                     /translation="MTKRFAALHAAGAFVLPGCPEVPPGDLLPVLDAQGGLHAWLAPF
                     VDAGHLPAYALLTPQLVLQRFSLFAGGRLDAAPAAADWLDPASIAARAEAAAGRGARA
                     EMPVLSFDGVPDRLAWRVALVFPDGQRQTWFVAGSAAWPASDAGEIEYRG"
     gene            complement(1112..2296)
                     /locus_tag="PLN02_09255"
     CDS             complement(1112..2296)
                     /locus_tag="PLN02_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOI52551.1"
                     /translation="MSFSLSAQDAHEFAITELNLLNAARALPDVDLEKSELMPGTPIF
                     DLNGEILFYRVPLEQADGQRGYVDIAAQSLFGESILALAPAADWNASTWLAQAKSVFA
                     RRARAARIADYDELRFVAYSYPKIAVQFLLDGKEVALLELATWAAIPTAEKRRKGGEP
                     GQFERWSLIAELPGKSKADAGKRFELRAAGLRKALGQRVGAGDLFISRDFLPATIAIS
                     RLHDSQQLRYSNRAGDHAPCFELRGQETSVWCVGASVQMLLDFYRYNYEQTRLAEELG
                     LVTLADPNGLPYGDENKVPQTLEKLTSSALSAAMVATPTFSLHVNEIRANRPLISFIP
                     GHSRAVAGYTRSLFALAGSPGFSGLLVYDPWPPNAGVITRWENFNTQTYRYAFTAHVN
                     TV"
BASE COUNT          472 a          808 c          810 g          410 t
ORIGIN      
        1 acctgggaag tcgaccgcga ccagttgttc aagaacaacg aagtgcatga catttccgtg
       61 gcggtcggcg tcgatccgca cggcatcgag aagatcgacg aggtcgatct gagcggcctg
      121 cgctacggcc gcatcatcgt catgtccgac gccgacgtgg acggctcgca catccaggtg
      181 ctgctgttca ccctcttcct caagcacttc ccggcgctgg tcgagaaagg ccacatccac
      241 gtcgcccagc cgccgctgtt ccgcgtcgat gtcgaggcca agggccacac gcgcaagatc
      301 tattgcctcg acgaggcgga gaaggaacag accctgcgca agctgcgcga cgaaggcgtc
      361 aatgaaaaca agatcaccgt ctcgcgcttc aagggcctgg gcgagatgaa cccggaccag
      421 ctctgggaaa ccacgctctg cccggacacc cgccgcctgg tgccgttcca ggccgagcgc
      481 gagacgatcc gccgcatgca ccagaccttc acgctgctga tgggcaaggg cgaagcgtcg
      541 ggccgccggc agtggatgga gaaggacggc gggctggtgg aaaccgacgt ctgaggcagg
      601 gcaacgccag ccttgccctc ctttcaacta cccccgatat tcaatctcac cggcgtcgga
      661 cgccggccac gccgcgctgc ccgcgacgaa ccaggtctgc cgctggccat ccgggaagac
      721 taacgccacc cgccaggcca gacgatcagg gacgccatcg aaggaaagca ctggcatttc
      781 cgcccgagca cccctacccg ccgcagcttc agcccgggcg gcgatgctcg ccgggtcgag
      841 ccagtctgcg gcagccggcg cggcatcgag ccgaccgcca gcgaacagcg agaatcgctg
      901 caggaccagt tgcggcgtca gcagggcgta ggccggcagg tgtccggcgt cgacgaaggg
      961 cgccagccag gcgtgcaagc cgccctgcgc atcgagtacc ggcagcaagt cgccgggtgg
     1021 cacttccgga cagccgggca ggacgaaagc gcccgcggca tggagggccg cgaagcgctt
     1081 cgtcactgcc ttgatgtcaa ccgccgtgat ttcagacggt attgacgtgc gcggtgaagg
     1141 catagcgata ggtctgcgtg ttgaagttct cccagcgcgt gatcacgccg gcattgggcg
     1201 gccagggatc atataccagc aggccggaaa acccgggact gccggccagc gcgaagagac
     1261 tgcgggtgta gccggcgacg gcgcgcgaat ggcccggaat gaagctgatc agcggccggt
     1321 tggcacggat ttcattgacg tgcagcgaaa aggtcggcgt tgccaccatc gccgcgctca
     1381 gcgccgacga ggtcagcttt tccagagtct ggggtacctt gttctcgtcg ccgtaaggca
     1441 ggccgttggg atcggccagc gttaccaggc cgagttcctc ggcgagtcgc gtctgctcgt
     1501 agttgtaacg atagaaatcg agcagcatct gcacgctggc gccgacgcac cagacgctgg
     1561 tttcctggcc gcgcaactcg aagcacggcg catgatcgcc ggcacggttg gaatagcgca
     1621 actgctggct gtcgtgcagg cggctgatgg cgatcgtcgc cggcaggaaa tcgcgcgaga
     1681 tgaagagatc gcctgcaccc acccgctgac ccagcgcctt gcgcaagccg gcggcccgca
     1741 gttcgaagcg tttgccggcg tcggccttgc tcttccccgg cagttcggcg atcagcgacc
     1801 agcgctcgaa ctgcccgggc tcgccgccct tgcgccgctt ttccgccgtt gggatcgccg
     1861 cccaggtagc cagttccagc agcgccacct ccttgccatc gagcaggaac tgaacggcga
     1921 tcttgggata gctgtaggcg acgaaacgca gttcgtcgta atcggcaatg cgggcggcgc
     1981 gggcgcggcg ggcaaaaacc gacttggcct gtgccagcca ggtgctggcg ttccagtcgg
     2041 cggccggcgc cagcgccagg atcgattcgc cgaacaggct ctgcgccgcg atatcgacgt
     2101 agccgcgctg accgtccgcc tgttccagcg gcacccggta aaacaggatt tcgccgttga
     2161 ggtcgaaaat cggcgttccc ggcatcagtt cgctcttttc cagatcgaca tcgggcagcg
     2221 cccgggccgc gttgagcagg ttgagttcgg tgatggcaaa ttcatgcgca tcctgcgcac
     2281 tgagggaaaa tgacatgatc gtttcctccg ttgtgttccg gtttttccgt gcctgccccc
     2341 gcctggtatc cttttccggg aagcggcaga ccatgctcat ggcgcccggc aatgtcatga
     2401 catggtttta aacatagaac aacggaatca tcagtcaatg aaaactttcg cgccgccagg
     2461 cggggccgga agggcgtcgt taccgaatgg cacagcgcgc
//
LOCUS       DAOSDX010000407         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14616_length_2500_cov_2.803272, whole genome shotgun sequence.
ACCESSION   DAOSDX010000407 DAOSDX010000000
VERSION     DAOSDX010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14616_length_2500_cov_2.803272"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /gene="gspK"
                     /locus_tag="PLN02_09260"
     CDS             <1..645
                     /gene="gspK"
                     /locus_tag="PLN02_09260"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HOI52552.1"
                     /translation="ELPPLRHEGARIGGRIVDLQGRFNLNNLRREDGRLDPQAFAAYR
                     RLLAILGLPDKLANALAERLTGTREGASDDAPAGGRPLLAFGELQGLAGYDAGVLARL
                     AGEVCVLAGSQPVNVNTAPAAVLSALQPGLSPAAAASLASGRRGLPFRDSGDFRNRLG
                     DPGLPGSLLPIAAASRHFLIEIEVDRDRARSRVTSLVRRNPNGRLPEIVWQTLL"
     gene            642..1796
                     /gene="gspL"
                     /locus_tag="PLN02_09265"
     CDS             642..1796
                     /gene="gspL"
                     /locus_tag="PLN02_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HOI52553.1"
                     /translation="MNQILTLIIPSGWPQETRACPWQLHAADGRLLAQGCAEPGQWPT
                     AGEDDRCHLLLAGRQVAAQPARLPPPPQGERQAVIAAALEDNLLEPAEHLLFALAPTP
                     LADGRRCVASVSRARLAALCQRLRELGWPPVAAWPLALALPTGQALALAGELSLRQDD
                     GFVALPLDATLPEWLDLLAPAAPIHASALDTDAAHALAAAGKRLQAEELPPLRIPDGP
                     GFLVDDLAPPRPHVDWVRRLRPALRLAAGLGALLLAMALGQWGWHAWQARQLRGEIAR
                     QFHEILPQTPMVDPVLQAQRQLDTRAQAGGALAANDFLLLADALSELAPEQLPLLALA
                     YADGRLRVSVAHLDAGAAERLAAAARQRGLTLSPPSGTPPEFTLSAGEHR"
     gene            1793..2296
                     /gene="gspM"
                     /locus_tag="PLN02_09270"
     CDS             1793..2296
                     /gene="gspM"
                     /locus_tag="PLN02_09270"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOI52554.1"
                     /translation="MKADLAPAWRRRAAVFWAARSPREQGILRLAAGVLALAIAGQLL
                     WQLEDARRRQLRLLPELAAQATQMAALAAAWQGLAATPLAPVDAAALRSVVQTRLGEL
                     GPEIAAHWEDDGRLTLRGKAALTAWLRWTAAVHEEQRLRLESCRITGSGETLQIEASY
                     RAAGKAS"
     gene            2293..>2500
                     /locus_tag="PLN02_09275"
     CDS             2293..>2500
                     /locus_tag="PLN02_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52555.1"
                     /translation="MRGGRLLLALLCLLLILAQLPAHLAARLLATACAEQCRLAATSG
                     TLWQGQGQLYVASGKDWYALGQLAW"
BASE COUNT          337 a         1016 c          825 g          322 t
ORIGIN      
        1 gaactgccgc cgctgcgcca cgaaggcgcc cgcatcggcg gtcgcatcgt cgacctgcag
       61 ggccgcttca acctcaacaa cctgcgccgc gaagacggcc gcctcgaccc ccaggcgttc
      121 gccgcctacc gccgcctgct ggccattctc ggcctgcccg acaagctggc caatgcgctg
      181 gccgaacgcc tgaccggcac acgagaaggc gccagcgacg acgccccggc gggcggacga
      241 ccgctgctcg ccttcggcga actacaaggc ctggctggct acgacgccgg cgtgctcgcc
      301 cgcctggccg gagaggtctg cgtcctcgcc ggcagccagc cggtcaacgt caatactgcg
      361 ccggccgccg tcctttcggc gctgcagccc ggtctctcgc cggccgccgc ggccagtctc
      421 gccagcggcc ggcgcggcct gcccttccgc gacagcggcg atttccgcaa ccgcctgggc
      481 gaccccggcc tgcccggcag cctgctgccg attgccgccg ccagccgcca cttcctgatc
      541 gaaatcgagg tcgaccgcga ccgggcgcgc agccgcgtca ccagtctggt ccgccgcaac
      601 cccaacggca gattgccgga aattgtctgg caaacccttc tatgaaccag atcctgaccc
      661 tgatcatccc ctccggctgg ccgcaggaaa cccgcgcctg cccctggcaa ctgcacgccg
      721 ccgacggccg cctgctggcg cagggctgtg ccgagccagg ccaatggccg accgccggcg
      781 aagacgaccg ctgccacctg ctcctcgccg gccgccaggt cgccgcccag ccggcccgcc
      841 tgccgccgcc cccgcagggc gaacgccagg cggtgatcgc cgccgcgctc gaagacaacc
      901 tgctcgaacc ggccgaacac ctgctgttcg cgctcgcccc gacgccgctg gccgatggtc
      961 gacgctgcgt cgccagcgtc agccgcgccc gcctcgccgc cctttgccag cgcctgcgcg
     1021 aactgggctg gccgcccgtc gccgcctggc cactggcgct ggcgctgccg accgggcagg
     1081 cgctggcgct cgctggcgaa ctgagcctgc gccaggacga cggcttcgtc gctttgccgc
     1141 tcgacgccac gctgcccgaa tggctggacc tgctggcgcc cgccgcgccg atccacgcca
     1201 gcgccctcga caccgacgcc gcgcacgccc tggcggcggc gggcaagcgc ctgcaggcgg
     1261 aagagctgcc gccgttgcgc atccccgacg gccccggctt cctggtcgac gatttggcac
     1321 cgccgcgccc ccatgtggac tgggtacgcc gcctgcggcc ggccttgcgc ctcgccgccg
     1381 gcctcggcgc gctactgctg gcgatggcgc tcgggcaatg gggctggcac gcctggcagg
     1441 cacgccagtt gcgcggcgaa atcgcccgcc agttccatga aatcctgccg cagacaccaa
     1501 tggtcgaccc cgtccttcag gcacagcgcc agctcgatac ccgggcgcag gccggcggcg
     1561 ccctggccgc caacgatttc ctgctgctcg ccgacgcgct cagcgagcta gcgccggaac
     1621 aactgccctt gctggcactg gcttacgccg acggccgcct gcgcgtcagc gtcgcccacc
     1681 tcgacgccgg cgccgccgag cgtctggccg ccgccgcccg gcagcgcggc ctgacgctga
     1741 gcccgccgag cgggacgccg cccgaattca ccctgagtgc cggagaacat cgatgaaagc
     1801 cgatctcgcc cccgcctggc ggcgccgcgc cgccgttttc tgggccgccc gctcgccgcg
     1861 cgaacagggc atcctgcgcc tcgccgccgg cgtcctggct ctggcaattg ccggccaact
     1921 gctgtggcaa ctcgaagacg cccgccgccg gcaactgcgc ctgctgccgg aactcgccgc
     1981 gcaggcgacg cagatggcgg cgctcgccgc cgcctggcaa ggtctggcgg cgacgccgct
     2041 cgctccggtc gacgcagcgg cgctgcgcag cgtcgtgcag acacggctgg gcgaactcgg
     2101 accggagatc gccgcgcact gggaggacga tggccggctg acgctacgcg gcaaggccgc
     2161 gctgaccgcc tggctacgct ggactgccgc cgtacacgag gaacaacggc tgcgcctgga
     2221 aagctgccgc atcaccggca gcggcgagac gctgcagatc gaggccagct atcgggccgc
     2281 cggcaaagcg tcatgagagg cggccgcctg ctgctcgcgc tgctctgcct gctcctgatc
     2341 ctcgcgcagt tgccggccca cctcgccgct cgcctgctcg ctacggcctg tgccgagcag
     2401 tgccgactgg cggcaacgag cggcacgctg tggcaaggcc aggggcagtt gtacgtcgcc
     2461 agcggcaagg actggtacgc cctcggccag ctcgcctggc
//
LOCUS       DAOSDX010000408         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14658_length_2494_cov_2.824108, whole genome shotgun sequence.
ACCESSION   DAOSDX010000408 DAOSDX010000000
VERSION     DAOSDX010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14658_length_2494_cov_2.824108"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PLN02_09280"
     CDS             complement(<1..133)
                     /locus_tag="PLN02_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOI52556.1"
                     /translation="MKPNDACPCGSGKSYAACCRPLHAGEKPASTAEALMRSRYAAYV
                     "
     gene            complement(130..1092)
                     /locus_tag="PLN02_09285"
     CDS             complement(130..1092)
                     /locus_tag="PLN02_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOI52557.1"
                     /translation="MWFKNLQIYRLPTPWAVDLAKLDEQLARGEFTRCPSNQPMSRGW
                     VSPRKDGALIYANNRQWLIALAVEQRLLPSSVVNETTQERAEQIADQQGYPPGRKQMK
                     EIKERVTEELMPRAFTRKRTSFVWIDPQNGWFVVDAGSPAKAEEVIEHLRHCLDEFPL
                     KPLHTQLSPQSAMADWLAGGDAPAGFTVDRDCELKSVAEEKAAVRYVRHPLGDEIGAE
                     IKAHLAAGKLPTRLALTWDERISFVLTEKLEIKRLAFLDILKEEAEKSAERADEQFDA
                     DFALMTGELVRFLPQLVAALGGEQVEAETPAAAPAAAGTPPWEA"
     gene            complement(1094..>1547)
                     /locus_tag="PLN02_09290"
     CDS             complement(1094..>1547)
                     /locus_tag="PLN02_09290"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HOI52558.1"
                     /translation="IAEKNLSNLRIGNHDAVEVVRDMLAEGALDGIHIFFPDPWHKTR
                     HKKRRLIQPPFVALLASRLKPGGYIHCATDWENYALQMLDVLGREPSLDNSVANPSTV
                     DLAAALDADSAACLAGFAPRPEYRPLTKFENRGLRLGHGVWDLVFRKR"
     assembly_gap    1548..1557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1558..1858)
                     /locus_tag="PLN02_09295"
     CDS             complement(<1558..1858)
                     /locus_tag="PLN02_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOI52559.1"
                     /translation="MIHPAAEGEEGEYTAGRHGHIKSFVRRAGRMSEAQQRYYETMLP
                     RIGIPYQPENIDLTAVFGRVAPKILEIGCGMGETTARIAEAHPENDYFGLEVHVPG"
     gene            complement(1870..>2494)
                     /locus_tag="PLN02_09300"
     CDS             complement(1870..>2494)
                     /locus_tag="PLN02_09300"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOI52560.1"
                     /translation="GQPSLLDAVPPSQFTILPNTAGCYTADDAVRTLRLARELLDGHP
                     LCKLEVLGDPTNLFPNMPETLKAAETLVKEGFEVMVYCADDPIQCKMLEEIGCVAVMP
                     LASLIGSGMGIVNPWNLRLIIDNAKVPIIVDAGVGTASDATIAMELGCDGVLMNTAIA
                     HARNPVLMASAMKKGIEAGREAFLAGRMPRKYYSADPSSPTSGLIGS"
BASE COUNT          363 a          775 c          885 g          461 t
ORIGIN      
        1 ggacgtaggc ggcgtagcgc gagcgcatca gcgcctcggc cgtcgacgcc ggtttttcgc
       61 cggcgtgcag tggcctgcag caggcggcgt aagacttgcc gctaccgcag ggacaggcgt
      121 cgttgggttt caggcttccc acggcggggt gccggcggcg gccggggccg cagcgggcgt
      181 ctcggcttcg acctgttcgc cgcccagggc tgcgaccagt tgcggcagga agcgcaccag
      241 ttcgccggtc atcagcgcga aatcggcgtc gaactgctcg tcggcacgtt cggcgctctt
      301 ttcggcttct tccttgagga tgtcgaggaa ggccaggcgc ttgatctcca gcttctcggt
      361 gaggacgaag ctgatccgct cgtcccaggt cagcgccagg cgcgtcggca gcttgccggc
      421 ggcaaggtgg gccttgatct cggcgccgat ttcgtcgccc agcggatggc ggacgtagcg
      481 cacggcggcc ttctcttcgg cgaccgattt cagctcgcaa tcgcggtcga ccgtgaaacc
      541 ggccggggcg tcgccgccgg ccagccagtc ggccatcgcc gattgcggcg agagttgcgt
      601 gtgcagcggc ttcagcggga attcgtcgag gcagtggcgc aggtgttcga tgacttcctc
      661 ggccttggcc gggctgccgg catcgacgac gaaccagccg ttctgcgggt cgatccagac
      721 gaagctcgtg cgcttgcggg tgaaggcgcg cggcatcagt tcctcggtga cgcgctcctt
      781 gatttccttc atctgcttgc ggccgggcgg gtagccctgc tggtcggcga tctgctcggc
      841 gcgttcctgc gtcgtttcat tgaccaccga cgacggcagc aggcgctgct cgacggccag
      901 cgcgatcagc cactggcggt tgttggcgta gatcagcgcg ccgtccttgc gcggcgacac
      961 ccagccgcgc gacatcggct ggttggacgg gcagcgggtg aattcgccgc gcgccagttg
     1021 ctcgtcgagc ttggccaggt cgaccgccca cggcgtcggc aggcggtaga tctgcaggtt
     1081 cttgaaccac atgtcagcgt ttcctgaata cgagatccca gacaccgtgg cccagacgca
     1141 ggccgcggtt ctcgaatttg gtcagcggcc ggtattccgg acgcggggcg aagccggcca
     1201 ggcaggcggc ggaatcggca tcgagcgcgg cggcgaggtc cacggtcgac ggattggcga
     1261 cgctgttgtc caggctgggc tcacgaccga gcacgtcgag catctgcagc gcgtagtttt
     1321 cccagtcggt ggcgcaatgg atgtagccgc ccggcttcag acgcgaggcg agcagcgcga
     1381 cgaagggcgg ctggatcaac cggcgcttct tgtgccgcgt cttgtgccag ggatcgggga
     1441 agaagatgtg gatgccgtcg agcgcgcctt cagccaacat gtcgcgcacc acttcgaccg
     1501 cgtcgtggtt gccgatgcgc agattgctca ggttcttctc ggcgatcnnn nnnnnnncgc
     1561 cggggacatg gacttccagc ccgaaatagt cgttctccgg gtgggcctcg gcgatgcgcg
     1621 ccgtggtttc gcccatgccg cagccgattt cgaggatctt cggcgctacg cggccgaaca
     1681 ccgccgtcag gtcgatgttt tcaggctggt agggaatgcc gatcctgggc agcatcgtct
     1741 cgtagtagcg ctgctgcgct tccgacatgc ggccggcgcg gcggacgaag ctcttgatgt
     1801 ggccgtggcg gccggcggtg tattcgcctt cctcgccttc ggcggcgggg tggatcagca
     1861 ggtcgtcgct cacgagccga tcaatccgct ggtcggcgac gagggatcgg ccgagtagta
     1921 cttgcgcggc atgcgaccgg ccaggaaagc ctcgcggccg gcctcgatgc ccttcttcat
     1981 ggcgctggcc atgagcaccg gattcctggc gtgggcgatg gcggtattca tcagcacgcc
     2041 gtcgcagccg agttccatgg cgatggtcgc gtccgaggcg gtgccgacgc cggcgtcgac
     2101 gatgatcggc accttggcgt tgtcgatgat caggcgcagg ttccacggat tgacgatgcc
     2161 catgcccgaa ccgatcagcg aagccagcgg catcaccgca acgcagccga tctcttcgag
     2221 catcttgcac tggatcggat cgtcggcgca gtaaaccatg acttcgaaac cttccttgac
     2281 cagggtttcc gcggccttca gcgtttccgg catgttcggg aagaggttgg tcgggtcgcc
     2341 gagcacttcg agcttgcaca gcgggtggcc gtcgagcagc tcgcgcgcca ggcgcaaggt
     2401 gcgcacggca tcgtcggcgg tgtagcagcc ggcggtattg ggcaggatgg tgaattgcga
     2461 cggcggcacg gcgtcgagca ggctgggctg gccg
//
LOCUS       DAOSDX010000409         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14689_length_2490_cov_2.763039, whole genome shotgun sequence.
ACCESSION   DAOSDX010000409 DAOSDX010000000
VERSION     DAOSDX010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14689_length_2490_cov_2.763039"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2288)
                     /locus_tag="PLN02_09305"
     CDS             complement(<1..2288)
                     /locus_tag="PLN02_09305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOI52561.1"
                     /translation="MTVPSALPDYAELHCLSNFSFLRGASHPEELAAQAVAQGYAALA
                     LTDECSLAGVVRVHQAFRAQGGTTRLVIGSEMTTVDGLKLVFLAQNREGYGNLSALIT
                     LARRRTEKGAYTLQRHDLNAISPNGALPDCLVLWVPEATPSVADGRWLAERFPGRCWI
                     AVELHAGPDDAARLADLQALGVACGLPLVAAGDVHMHVRARRPVQDVLTALRLKTSVF
                     DAGHALFPNGERHLRSRLRLARLYPPELLAETLRIAEKCTFSLDELRYEYPEEIVPAG
                     HTPTSWLRYETEAGLRRRYPAGVPDAVRERIEHELRLIAELQYEAYFLTVYDIVCFAR
                     GAGILCQGRGSAANSTVCFALGVTEVDPARSAMLFERFVSKERGEPPDIDVDFEHERR
                     AEVIAYIYEKYGRERTALAAAVITYRTKGALRDAGRALGFDIARIDALTASLAWWDKR
                     EQMPERFAEQGLDPASPRVAKWLWLAEQLRGFPRHLTQHVGGFVMSRGPLARLVPVEN
                     TAMAERTVIQWDKDDLDAVGLMKVDVLALGMLSALRRMLAMIGPLALNDIPEGDKATY
                     DMLCRADSMGVFQVESRAQMSMLPRLKPRNYYDLVIEVSLVRPGPIQGDMVHPYLKRR
                     QGKEDIERITPAIDAVLARTCGVPIFQEQVMQLAIVAAGFTPGEADELRRAMAAWRRK
                     GNLARYQDKLRAGMARNGVPAAFVERIVAQIQGFGEYGFPESHAASFALLVYASAWVK
                     RHHPAAFLCGLLNSQPMGFYSPS"
BASE COUNT          418 a          857 c          850 g          365 t
ORIGIN      
        1 gacggcgaat agaaacccat cggctggctg ttgagcaggc cgcacaggaa ggccgccggg
       61 tggtggcgct tgacccaggc cgaggcatag accagcagcg cgaagctggc ggcgtgcgac
      121 tcggggaagc cgtattcgcc gaagccctgg atctgggcga caatgcgttc gacgaaggcg
      181 gccggcacgc cgttgcgcgc catgccggcg cgcagcttgt cctggtagcg ggcgagattg
      241 cctttacgcc gccaggccgc catcgcccgc cgcaattcgt cggcctcgcc gggggtgaag
      301 ccggcggcga cgatggccag ttgcatgacc tgttcctgaa agatcggcac gccgcaggtg
      361 cgcgccagca ccgcgtcgat cgccggcgtg atgcgctcga tatcctcctt gccctggcgc
      421 cgcttcaggt agggatggac catgtcgccc tggatcggcc cgggccgcac cagcgagact
      481 tcgatcacca ggtcgtaata attgcgcggc ttcaggcgcg gcagcatgct catctgcgcc
      541 cgcgattcga cctggaagac acccatgctg tcggcgcggc agagcatgtc gtaggtcgcc
      601 ttgtcgcctt cggggatgtc gttcaatgcc agcggcccga tcatcgccag catccggcgc
      661 agcgccgaca gcatgccgag cgccagcacg tcgaccttca tcaggccgac cgcgtcgagg
      721 tcgtccttgt cccactggat caccgtgcgc tcggccatcg ctgtgttctc gaccggcacc
      781 agccgggcca gcggcccgcg cgacatgacg aagccgccga cgtgctgggt caggtggcgg
      841 ggaaagccgc gcagctgctc ggccagccac agccacttgg cgacgcgcgg cgacgccggg
      901 tccagcccct gttcggcgaa gcgttccggc atctgttcgc gcttgtccca ccaggcgagc
      961 gaggcagtca gcgcgtcgat gcgggcgatg tcgaagccga gcgcgcggcc ggcgtcgcgc
     1021 agcgcgccct tggtccggta ggtgatgacg gcggcagcca gcgccgtgcg ctcgcgcccg
     1081 tatttttcgt aaatgtaggc gatgacctcg gcgcgccgct cgtgttcgaa atcgacgtcg
     1141 atgtcgggcg gctcgccacg ctctttcgag acgaagcgct cgaacagcat cgccgagcgc
     1201 gccggatcga cctcggtgac gccgagcgcg aaacagaccg tcgaattcgc cgccgagccg
     1261 cgcccctggc agaggatgcc ggcgccgcgg gcgaagcaga cgatgtcgta gacggtcagg
     1321 aaataggctt cgtactgcaa ctcggcaatc agccgcagtt cgtgctcgat gcgctcgcgc
     1381 accgcgtccg gcacgcccgc cggatagcgc cggcgcagcc cggcctcggt ttcatagcgc
     1441 aaccacgagg tcggcgtgtg gccggcggga acgatttcct ccgggtattc gtagcgcagc
     1501 tcgtcgagcg aaaacgtgca tttttccgcg atgcgcagcg tctcggccag cagctcgggc
     1561 ggatagagcc gcgccagccg caggcgcgag cgcaagtggc gctcgccgtt cgggaacagc
     1621 gcgtggccgg cgtcgaacac cgaggtcttc aggcgcagcg cggtcagcac gtcctgcacc
     1681 ggccgccgcg cccgcacgtg catgtgcacg tcgcctgcgg ccaccagcgg caggccgcag
     1741 gcgacgccga gcgcctgcag gtcggccagc ctggccgcat cgtccgggcc ggcgtgcagt
     1801 tcgacggcga tccagcaacg tccgggaaaa cgttcggcca gccagcgccc gtcggcaacc
     1861 gagggcgtcg cctcgggcac ccacagcacc aggcaatcgg gcaaggcgcc attcggcgag
     1921 atggcgttca ggtcgtggcg ctgcaaggta taggcaccct tctccgtccg ccgccgggca
     1981 agcgtgatca gcgccgagag gttgccgtaa ccctcccggt tctgcgccag aaaaaccagt
     2041 ttcaggccat cgaccgtggt catctcgctg ccgatgacga ggcgcgtcgt cccgccctgc
     2101 gcccggaacg cctgatgcac gcgcacgacg ccggccagcg agcactcgtc ggtcaacgcc
     2161 agcgccgcgt aaccctgcgc caccgcctgc gccgccagct cctccgggtg cgaggcaccg
     2221 cgcaggaagc tgaagttgga caggcagtgc agctcggcgt agtcggggag cgcggacggg
     2281 acagtcaatg cgggcccctg gcaaacaact gtattttcat acagtcattt tagcccagcc
     2341 cgcgtttcag tcccagcgct gctggtagga aaaagtcggc agcttccagg cgaagcgcag
     2401 cgccagcatg cggaaggcga aaccggcgcc gaaacagagc agcgtgatca ggtcgctgtc
     2461 ccagccgagc ccgcgcagca ccaggaagag
//
LOCUS       DAOSDX010000410         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14712_length_2487_cov_3.105263, whole genome shotgun sequence.
ACCESSION   DAOSDX010000410 DAOSDX010000000
VERSION     DAOSDX010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14712_length_2487_cov_3.105263"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLN02_09310"
     CDS             <1..801
                     /locus_tag="PLN02_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOI52562.1"
                     /translation="INCAAIPDSLLEATLFGYEKGAFTGAQQAQAGKFEQAQDGTLLL
                     DEVTEMPLGLQAKLLRVLQEREVERVGGKKPVALNIRIVATSNRDMAEAVARGVFRED
                     LYYRLNVFPVAIPALCQRPLDIVPIARHFIAEHGSRFGRTGMRLSPAAERALTVHAWP
                     GNVRELENVIQRALILAPGQEIAAEHLNLPANAVVPAAATAVGAGQPVAAKNGDNRNI
                     KDLEREHILQTLAEVGGSRKLAIERLGISERTLRYKLQQYREEGHYQD"
     gene            849..1175
                     /gene="fliE"
                     /locus_tag="PLN02_09315"
     CDS             849..1175
                     /gene="fliE"
                     /locus_tag="PLN02_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOI52563.1"
                     /translation="MDTKGIEQMLSTLRATAAQASGLPAAGNSGTTAGVTDFAEVLKN
                     SIDKVNEAQKSANQMAEKLAAGDSNQNLHEVMIALQSASVSFQEMVQVRNKLVTAYQD
                     VMNMQV"
     gene            complement(1187..1978)
                     /locus_tag="PLN02_09320"
     CDS             complement(1187..1978)
                     /locus_tag="PLN02_09320"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOI52564.1"
                     /translation="MPTVSNTASTTLFEIDQPDQFGQFIQSNPLEILRNLHALTRQGG
                     FVSVYFDRGEKFFLSTILAVDESRRSIVIDAPRQAEVLQQAMHADIATLCANLERIKI
                     QFHLQRLQPNTWEDREALISPLPEKMLRLQRREFFRIQTPLLNPLRCRLIRQHGDGRS
                     EVFEFPLHDISGGGVCLIAPIEMAEKFSLGELFADCRLDIPGESVLSVNLRVRELAQL
                     EMHDGQPQLRLGCEFFNLPGTRLSLIERYITRQERENKARLNGYV"
     gene            complement(2001..2297)
                     /locus_tag="PLN02_09325"
     CDS             complement(2001..2297)
                     /locus_tag="PLN02_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOI52565.1"
                     /translation="MSPTAKPNPLREAVALAYSQTEAAPRVVAKGKGLIAEQIIAKAR
                     EHGVYVHESAELVALLTQVDIDEHIPPQLYMAVAELLAWLYRIEQGEHPEPPKV"
     gene            complement(2263..>2487)
                     /locus_tag="PLN02_09330"
     CDS             complement(2263..>2487)
                     /locus_tag="PLN02_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOI52566.1"
                     /translation="RLKLTLPVLGEVDASISLQGSGQISLRLAAGDAASATQLQERLA
                     ELRRQFETAGLSIGQMRVDHEPHREAEPAA"
BASE COUNT          466 a          725 c          819 g          477 t
ORIGIN      
        1 atcaactgcg cggcgatccc cgacagcctg ctcgaagcga cgctgttcgg ctacgagaaa
       61 ggcgccttca ccggggcgca gcaggcccag gccggcaagt tcgagcaggc gcaggacggt
      121 accctgctac ttgacgaagt cacggaaatg ccgctggggc tgcaggccaa gctgctgcgg
      181 gtgctgcagg aaagggaagt cgagcgggtc ggcggcaaga agccggtggc gctcaacatc
      241 cgcatcgtcg ccacgtccaa ccgcgacatg gcggaagcgg tggctcgcgg cgtctttcgc
      301 gaagacctct actaccggct caacgtcttc ccggtggcga ttccggcctt gtgccagcgg
      361 ccgctcgata tcgtgccgat cgcccgccat ttcatcgccg agcatggcag tcgtttcgga
      421 cgcaccggca tgcgcctgtc gccggcggcg gagcgggcgc tcactgtgca cgcctggccg
      481 ggcaacgtcc gggagctgga aaacgtcatc cagcgcgcgc tgatcctggc gccggggcag
      541 gaaatcgcgg cggaacattt gaacctgccg gctaacgcgg tggtgccggc ggcggcgacg
      601 gcggttggcg ccgggcagcc ggttgccgcg aaaaacggcg ataatagaaa catcaaggat
      661 ctcgaacgcg agcacatcct gcagacgctg gccgaagtcg gcggttcgcg caagctggcg
      721 atcgagcgtc tggggatttc cgagcggacc ttgcgctaca agttgcagca gtatcgcgag
      781 gaaggtcact atcaggactg atattggcct gcttcttgct ctgaattgcc tgcgaaaagg
      841 agtgacagat ggacaccaag ggtatcgaac agatgttgag cacgttgcgg gcaaccgccg
      901 cgcaggcctc tggcctgccc gctgccggga attccggcac gacggccggc gtgaccgatt
      961 ttgccgaggt gctgaaaaat tccatcgaca aggtcaacga ggcccagaaa tcggccaatc
     1021 agatggcgga aaaacttgcc gccggtgaca gcaaccagaa tctgcacgaa gtcatgatcg
     1081 ccctgcagag cgccagtgtc tcgttccagg aaatggttca ggtccgcaac aagctcgtca
     1141 cggcctatca ggacgtgatg aacatgcagg tctgagcgct ggccgatcag acgtagccgt
     1201 tgagtcgggc tttgttctcg cgttcctgcc gggtgatgta gcgctcgatc aggcttagtc
     1261 gggtgcccgg caggttgaag aactcgcacc ccagtcttag ctgcggctga ccgtcgtgca
     1321 tttccagctg ggccagttcg cgcacccgga gattgaccga gagcacgctt tcgccgggaa
     1381 tgtccaggcg gcaatcggca aacagttcgc ccagggaaaa tttttccgcc atctcgatcg
     1441 gtgcgatcag acagacgccg ccgccgctga tgtcgtgcag cgggaactcg aagacctcgc
     1501 tacggccatc gccgtgctgg cgaatcaggc ggcagcgcag gggattgagt agcggtgtct
     1561 ggatacggaa gaattcgcgg cgctgcaggc gcagcatttt ttccggcagg gggctgatca
     1621 gcgcttcgcg gtcttcccag gtatttggct gcagacgctg cagatggaac tgaatcttga
     1681 tccgttccag attggcgcac agggtagcga tgtcggcgtg catcgcttgc tgcaggactt
     1741 ccgcttggcg tggggcatcg atcacgatgc tgcgtcgact ttcgtcgacg gccagtatcg
     1801 tgctcaggaa aaatttttca ccgcgatcaa agtaaacact gacgaatccc ccctgtctgg
     1861 tcagcgcgtg cagattgcgc aggatttcca gcgggttgct ctggatgaac tggccgaact
     1921 ggtccggttg gtcgatttcg aataaggtcg tggaagcggt gttgctgacg gtgggcatgg
     1981 tggcgctttg ctcgatcggg ttagaccttg ggtggttccg gatgctcgcc ttgttcgatc
     2041 cggtacagcc aggccagtag ttcggcgacg gccatgtata actgcggcgg gatgtgttcg
     2101 tcaatatcga cctgggtcag caaggcgacc agttcggccg attcgtggac gtaaaccccg
     2161 tgttccctgg ctttggcgat gatctgctcg gcgatcagcc ccttgccctt ggcgacaacg
     2221 cggggcgcgg cttcggtctg gctgtaggcc agtgcaaccg cttcacgcag cgggttcggc
     2281 ttcgcggtgg ggctcatgat cgacacgcat ctggccaatc gacagaccgg cggtttcaaa
     2341 ttgccgacgc aactcggcca ggcgttcctg caactgcgtc gcgcttgccg catcgccggc
     2401 ggccaggcgc aggctgatct gccccgagcc ttgcagcgag atactggcgt caacctcacc
     2461 gagaacgggg agtgtcagtt tcaggcg
//
LOCUS       DAOSDX010000411         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14713_length_2487_cov_2.924753, whole genome shotgun sequence.
ACCESSION   DAOSDX010000411 DAOSDX010000000
VERSION     DAOSDX010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14713_length_2487_cov_2.924753"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLN02_09335"
     CDS             complement(<1..247)
                     /locus_tag="PLN02_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52567.1"
                     /translation="MPTPPSQFTRIRLIDLRWFSIGGMLLAATVSTSVLGSADLLPRL
                     FAFTLVVACLNGCLWLASRLHSGDDIPLISPWTQLQFD"
     gene            complement(387..956)
                     /locus_tag="PLN02_09340"
     CDS             complement(387..956)
                     /locus_tag="PLN02_09340"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOI52568.1"
                     /translation="MKQLVSRLRALIPTREQIMTNRWLRWLHPFLAHPRLWHWSRRGV
                     ALGVAIGIFFGLLIPIAQIPFSAAAAILLRANVPAAAASTLVTNPVTFGPVYYAAYKL
                     GVWIHGGDHAAAPPATQLNGNQETGVLQRIRRLGLPLLTGLAVMASLFGLLSYVVISA
                     VWYWRVKARRRRQLRDYQTRARGRIHGPG"
     gene            1193..1777
                     /locus_tag="PLN02_09345"
     CDS             1193..1777
                     /locus_tag="PLN02_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI52569.1"
                     /translation="MNQPVHLRAHKLSASASLCAAGFNQLAADSPAIEAMTDFSRVAA
                     VSIAPEALIDDANARMISRGVRLLMVLGKDDEVVGLITARDILGEKPLQVALARASKR
                     DELRVGELMTPIGSIDTLALADVLRASVGEILDALKQLGRQHILVEDNDPLTGLPRVR
                     GLFSATQIGRALGVPVLGFDLPRTFAEIEAALVN"
     gene            1861..>2487
                     /locus_tag="PLN02_09350"
     CDS             1861..>2487
                     /locus_tag="PLN02_09350"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOI52570.1"
                     /translation="MAGGISSNPAGKVMSARTAVARIRDGDTVATGGFVGIGFAENIA
                     VALEQRFLEARESDVHGLGSPRGLTLVYAAGQGDGKERGLNHLGHDGLVDRVIGGHWG
                     LAPRLQELAVANRVEAYNLPQGVITHLFRDIAAGKPGTISRIGLGTFVDPRFGGGRLN
                     QRTTTEIVRLMEIDGEEYLFYKAFPIHVGIIRGTTADPDGNVTMEKEAL"
BASE COUNT          441 a          807 c          888 g          351 t
ORIGIN      
        1 cgtcgaactg cagttgcgtc cacggcgaaa tgagcgggat gtcgtcgccg ctatgcaggc
       61 gcgatgccag ccacaggcag ccgttgaggc aggcgacgac gagggtgaag gcgaacagcc
      121 ggggcagcag gtcggcgctg ccgaggacgc tggtgctgac cgtggcggcg agcagcatgc
      181 cgccgatcga gaaccagcgc aggtcgatca ggcgtatccg ggtgaactgg gaggggggcg
      241 tcggcacggg caaaaaagaa gccccggggt cggggctgaa gggatcgaga ggtgttcgct
      301 acaaacactg atccacacaa taacgccagg cgccgcggct gtggagtgac cgaatgtcgc
      361 acccaccccg gggcggtcgg cggaggtcag ccggggccgt ggatgcggcc gcgcgcccgc
      421 gtctggtaat cgcgcagctg gcgccgccgc ctggccttga cccgccagta ccagacggcg
      481 ctgatcacga cgtagctgag cagtccgaac agcgaggcca tgacggcgag cccggtgagc
      541 agcggcagac caaggcggcg aatgcgttgc aggacgccgg tttcctggtt gccgttaagt
      601 tgagtcgcgg gtggagcggc ggcgtggtcg ccgccatgga tccagacgcc gagcttgtag
      661 gcggcgtaat agacggggcc gaaggtcacc gggttggtga ccagcgtgct cgccgcggcg
      721 gccggcacgt tggcgcgcag caggatggcg gcggcagccg agaagggaat ctgggcgatc
      781 ggaatgagca ggccgaagaa gatgccgatc gccacgccga gcgcgacgcc gcggcgcgac
      841 cagtgccaca gccggggatg ggcgaggaag ggatggagcc agcgcagcca gcggttcgtc
      901 atgatctgct cccgggtcgg gatgagggcg cgcaggcggc tgacgagttg cttcatggcg
      961 gggtacggcg tctttcggcg aaggatcggg ggcggcaaat cataccgttt cggcaatccg
     1021 gcgggggccg ccgatggtcc ttcgaggcgc tgtcgggcgc cattttcgcg ctacttgcaa
     1081 cgcggccggc ttggctcaat actgtgcgac agaaggtcgc ggcggcaagg cgagcttccg
     1141 gacaacagcg gcgtcagccg caagacaata atcctgatgg aggaagacaa ccatgaacca
     1201 acccgttcac ctgcgtgccc acaaactgtc ggccagcgcc agcctgtgcg ccgccggttt
     1261 caaccaactc gccgccgatt cgccggcgat cgaggcgatg accgatttct cccgggtcgc
     1321 cgcggtcagc atcgcgcccg aggcgctgat cgacgacgcc aacgcccgca tgatctcgcg
     1381 cggggtccgc ctgttgatgg tgctcggcaa ggacgacgag gtggtcggcc tgatcaccgc
     1441 ccgcgacatc ctcggcgaga agccgctgca ggtcgccctg gcgcgcgcca gcaagcgcga
     1501 cgaactgcgt gtcggcgaac tgatgacgcc gatcggcagc atcgacacgc tggccctggc
     1561 cgacgtgctg cgcgccagcg tcggcgaaat cctcgatgca ctgaagcagc tcgggcgtca
     1621 gcacatcctg gtcgaggaca acgacccgct gaccggtctg ccgcgcgtcc gtggcctctt
     1681 ctcggcgacc cagatcggtc gcgcgctcgg cgtgccggta ctcggcttcg acctgccgcg
     1741 cacctttgcc gagatcgagg cggcgctggt caactgagcc ggcacaactc agggtaaacg
     1801 agcgtggcgc ggcgccacgc tctgacccaa catgagcgtt ttctgcgggc ggaggaaacg
     1861 atggcgggtg gtatttccag caatccggcg ggcaaggtga tgtcggcgcg caccgccgtc
     1921 gcccgcatcc gcgacggcga caccgtcgcc accggcggct tcgtcggcat cggcttcgcc
     1981 gagaacatcg ccgtcgccct cgaacagcgc tttctggaag cgcgcgagag cgacgtgcac
     2041 ggtctcggca gcccgcgcgg cctgaccctg gtctacgcgg ccgggcaggg cgacggcaag
     2101 gaacgcggcc tcaaccatct cggccacgac ggcctggtcg accgcgtcat cggcggccac
     2161 tggggactgg cgccgcgcct gcaggaactg gccgtggcca accgggtcga ggcctacaac
     2221 ctgccgcagg gcgtcatcac ccatttgttc cgcgacatcg ccgccggcaa gccggggacg
     2281 atcagccgca tcggcctcgg caccttcgtc gatccgcgtt tcggcggcgg caggctgaac
     2341 cagcgaacga ccaccgagat cgtccgcctg atggaaatcg acggcgagga atacttgttc
     2401 tacaaggcct tcccgatcca cgtcgggatc atccgcggca ccaccgccga tcccgacggc
     2461 aacgtcacca tggaaaagga ggcgctg
//
LOCUS       DAOSDX010000412         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14715_length_2487_cov_2.476151, whole genome shotgun sequence.
ACCESSION   DAOSDX010000412 DAOSDX010000000
VERSION     DAOSDX010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14715_length_2487_cov_2.476151"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLN02_09355"
     CDS             complement(<1..596)
                     /locus_tag="PLN02_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOI52571.1"
                     /translation="MNDQFDLFSQTPAVPDETPAAPALAPAAVVTEAAPPPASDDADL
                     PPLPPAAQGPAEDIPPPADYAARRYLEYAMSVVTGRALPANADGQKPVQRRILYAMHR
                     MGLYKSPKHVKSARVVGDVIGKYHPHGDSSVYDAMVRMAQDWSLRYPVVDGQGNFGSR
                     DGDNAAAMRYTEARLTPIAELLLSELDEGTVEWKPNYDG"
     gene            748..2103
                     /locus_tag="PLN02_09360"
     CDS             748..2103
                     /locus_tag="PLN02_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOI52572.1"
                     /translation="MTSSQGPRAHWSSRWAFFLVTVGSAVGLGNIWKFPYMTGSNGGS
                     AFVLVYLACILVIGVPLLMAETLLGRRGQGNPVMTMRVLADEAGASRWWQVIGLIGIV
                     GAFIILAFYSVVAGWVLEYTWRAGLGFAVQPKEAFADAFGALLASPGRLIFWHTVFMA
                     LTAAVVLGGVTSGIERANKIMMPALFGILVFLVAYGAIVADLPAAFRFLFHFDPSAIN
                     RGVLFSAMGHAFFTLSLGMGAIITYGSYLGREVSIPRTCLAIAAADTGVALLAGLSIF
                     AIVFAQGLEPAAGPGLLLQTLPLAFSQMPGGHIVGLVFFVLVVFAAWTSAISLLEPGV
                     TLLVEHFDLGRKAAVLLLSTGIWLLGVAVALSFNEWSAFSLFGLGLFDLLDTLTTKIM
                     MPLAGLLIALFTAWAMKRAHVEEEVGLRGGAFRLWFGVVRYVSPVAIVLIFLNVIGVI
                     G"
     gene            complement(2114..>2487)
                     /locus_tag="PLN02_09365"
     CDS             complement(2114..>2487)
                     /locus_tag="PLN02_09365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOI52573.1"
                     /translation="DIDHFKSINDRFGHEAGDQALVEVARRLGGMLRQEDTLARIGGD
                     EFVILLSDLAENAERIVAKVAEKCLSIFEPDFVIGGENCRLSTSIGIALGDADGDQLL
                     IAADQAMYRAKEQGRGRYRWA"
BASE COUNT          310 a          822 c          851 g          504 t
ORIGIN      
        1 ccgtcgtaat tgggcttcca ctccaccgtg ccttcgtcga gttcgctgag cagcagttcg
       61 gcgatgggcg tcagccgcgc ttcggtgtag cgcatggcgg cggcgttgtc gccgtcgcgc
      121 gagccgaagt tgccctggcc gtcgactacc gggtagcgca ggctccagtc ctgcgccatg
      181 cgcaccatcg cgtcgtacac cgacgaatcg ccgtgcgggt ggtatttacc gatcacgtcg
      241 ccgacgacgc gcgccgactt gacgtgcttc gggctcttgt acaggcccat ccggtgcatc
      301 gcgtagagga tgcgccgctg caccggtttc tggccgtcgg cgttggccgg cagggcgcgg
      361 ccggtgacca cgctcatcgc gtattcgagg tagcggcggg cggcgtagtc ggccggcggc
      421 ggaatgtcct cggccgggcc ctgggcggcg ggtggcagcg gcggcaggtc ggcgtcgtcg
      481 ctggccggcg gcggggcggc ctcggtcacc acggctgcgg gggcgagggc tggcgcggca
      541 ggggtttcgt ccggcacggc gggggtttgg gagaagaggt cgaattggtc gttcatggtg
      601 gcgactgcag ggattccggt cgggggcgaa tgtatcacgc gggctcggcg cgtggcgctt
      661 gcgcgggtcc tgtctaggtg aaatccccaa gtttgcgcgc gcgccggggc gcgtagaatc
      721 ccgcccctta aaattccgga cagcatcatg acttcctcgc aagggccgcg cgcccactgg
      781 tccagtcgct gggccttctt cctggtcacc gtcggctcgg cggtcggtct tggcaacatc
      841 tggaaatttc cctacatgac cggcagcaac ggcggcagcg ccttcgtgct ggtctacctg
      901 gcctgcatcc tggtcatcgg cgtgccgctg ctgatggcgg agaccctgct cggccggcgc
      961 ggtcagggca atccggtgat gacgatgcgg gtgctcgccg acgaggccgg cgccagccgc
     1021 tggtggcagg tgatcggcct gatcgggatt gtcggcgcct tcatcatcct ggcgttctat
     1081 agcgtggttg ctggctgggt gctggaatac acctggcgcg ccggtctcgg tttcgcggtg
     1141 cagcccaagg aagcctttgc cgacgccttc ggcgccttgc tggccagccc cgggcggctg
     1201 atcttctggc atacggtgtt catggcgctg accgcagcgg tggttctcgg cggggtgacc
     1261 agcggcatcg agcgcgccaa caagatcatg atgccggcgc tgttcggcat cctcgttttc
     1321 ctggtcgctt acggcgccat cgtcgccgac ctgccggcgg ccttccgctt cctgttccat
     1381 ttcgacccgt cggcgatcaa ccgcggcgtg ctgttctccg ccatgggcca tgctttcttc
     1441 acgctcagcc tgggcatggg ggcgatcatc acctacggtt cctacctcgg ccgcgaggtg
     1501 tcgattcccc gtacctgcct ggcgatcgcc gccgccgaca ccggcgtcgc gctgctcgcc
     1561 ggcctgtcga tcttcgccat cgtcttcgcc caggggctgg aaccggccgc cggccccggc
     1621 ctgctgctgc agaccctgcc gctggccttc tcgcagatgc cgggcgggca catcgtcggc
     1681 ctggtgttct tcgtgctggt cgtctttgcc gcctggacct cggccatctc gctgctcgag
     1741 cctggcgtca ctctgctcgt cgagcatttt gacctcgggc gcaaggcagc ggtgctgctg
     1801 ctgtcgaccg gcatctggct gctcggcgtc gccgtcgcgc tgtcgttcaa cgaatggagc
     1861 gctttcagcc tgttcgggct cggcctgttc gacctgctcg acacgctgac caccaagatc
     1921 atgatgccgc tggccggtct gctgatcgcg ctgtttaccg cctgggcgat gaagcgcgcc
     1981 cacgtcgaag aggaagtcgg cctgcgcggc ggcgccttcc gactctggtt cggcgtcgtc
     2041 cgttacgtct cgccggtggc gatcgtcctg atcttcctca acgtcatcgg cgtcattggc
     2101 tgagccacct ggttcaagcc cagcgatagc gaccgcgccc ctgttccttg gcgcggtaca
     2161 tcgcctggtc ggcggcgatc agcaactggt caccgtcggc atcgccgagg gcgataccga
     2221 ttgacgtcga gagtcggcaa ttctcgccgc cgatgacgaa atccggctcg aaaatgctca
     2281 ggcacttttc ggcgaccttg gccacgatgc gctcggcgtt ttccgcgagg tcggacagca
     2341 ggatcacgaa ctcgtcgccg ccgatgcgcg ccagcgtgtc ttcctggcgc agcatgccgc
     2401 cgagccggcg ggcgacttcg accagagcct ggtcgccggc ttcgtgaccg aaacggtcgt
     2461 tgatgctctt gaagtggtcg atgtcga
//
LOCUS       DAOSDX010000413         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14762_length_2481_cov_2.690849, whole genome shotgun sequence.
ACCESSION   DAOSDX010000413 DAOSDX010000000
VERSION     DAOSDX010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14762_length_2481_cov_2.690849"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="PLN02_09370"
     CDS             complement(<1..1692)
                     /locus_tag="PLN02_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOI52574.1"
                     /translation="MSTSLKTLISKLNTTCRQATERAAGLCMARGNYEVDFEHLLLAL
                     LEQQDSDLTTILRRCKISPSGLERELHDEVGRFRNGNTRTPVFSTHIIKLFEQAWLIA
                     SLDTRLPRIRSGHLLLALLTEPELAQLATRGAHLLAKVRLDELKHKFDEITEGSAEAA
                     EVTGLSGKPAEEEGGDPLQGSAPQAGKTPALDQFTTNLTQRARDGKVDPVIGRDAEIR
                     QAIDILMRRRQNNPILTGEAGVGKTAVVEGLALRIAAGDVPPPLAEVELHSLDMGLLQ
                     AGASVKGEFENRLKNVIDEVKKSPKPIILFIDEAHTMIGAGGQSGQNDAANLLKPALA
                     RGELRTVAATTWAEYKKYFEKDAALARRFQVVKVEEPSEPLAAAMLRGMVPLMEKHFG
                     IRVMDEAVTEAVRLSHRYISGRQLPDKAIGVLDTACAKVALGQSANPALIDETEKRLL
                     RERAELAALEREAASGAWHDTRIGELKAQIAALESDLAIHRARFDEEKALVARILELR
                     AALEAGEPSPAADAEKARKPARRSKKAGSPTSGNAELDQAMARLREVQGETPMVPL"
     gene            complement(1717..>2481)
                     /gene="tssG"
                     /locus_tag="PLN02_09375"
     CDS             complement(1717..>2481)
                     /gene="tssG"
                     /locus_tag="PLN02_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HOI52575.1"
                     /translation="MLGVNGTLPVHYTEQVIDHERFKRDTSGRAFLDLFTNRAVGHFY
                     RAWKKYRLPIQYETNRRNRFLPLILALGGVGFDALRDRLNDAPGAIEDESIAYFTTLL
                     RQRPVSGEAMARVLAAYFRTPVRVEQFVGRWYVVPRAQWTTLGGQNCALGVDSLVGER
                     VWQRNLRIRLHIGPMAHEQYMGFMPRGELAHALEKLLELATGGQFEYEIRPILRAPDV
                     RPVSLNGGRLGYDSFLISRPETRDRSDCRYLTQTIH"
BASE COUNT          351 a          757 c          904 g          469 t
ORIGIN      
        1 cagcggcacc atcggcgtct cgccctgcac ttcgcgcagg cgggccatcg cctggtcgag
       61 ttcggcattg ccggaggtcg gcgaaccggc cttcttgctg cggcgcgcgg gtttcctggc
      121 cttttccgcg tcggccgcgg gcgacggctc gccggcttcg agcgcggcgc gcagttcgag
      181 aatgcgggcg acaagcgcct tttcctcgtc gaagcgggcg cggtggatgg ccagatcgct
      241 ttccagcgcg gcaatctgcg ccttcagttc gccaatccgc gtgtcgtgcc aggcgccgct
      301 ggcggcttcg cgttcgagcg cggcgagttc ggcgcgctcg cgcagcaggc gcttctcggt
      361 ttcgtcgatc agcgccgggt tggcgctctg gccgagggca accttggcgc aggcggtgtc
      421 gagaacgccg atcgccttgt ccggcagttg ccggccgctg atgtagcggt gcgacaggcg
      481 gacggcttcg gtgacggctt cgtccatgac gcggatgccg aagtgctttt ccatcaacgg
      541 caccatgccg cgcagcatgg cggcggcgag cggctcggac ggttcttcga ccttgaccac
      601 ctggaagcgg cgggcaagcg ccgcgtcctt ctcgaagtac ttcttgtatt cggcccaggt
      661 ggtggcggcg acggtgcgca gttcgccgcg cgccagcgcc ggcttgagca ggttggcggc
      721 gtcgttctgg ccggactggc cgccggcgcc gatcatggtg tgggcttcgt cgatgaacag
      781 gatgatcggc ttcgggcttt tcttgacctc gtcgatgacg ttcttcaggc ggttctcgaa
      841 ctcgcccttg acgctggcgc cggcttgcag caggcccatg tcaaggctgt gcagttcgac
      901 ctcggcgagc ggcggcggca cgtcgccggc ggcgatgcgc agggccaggc cctcgaccac
      961 ggcggtcttg ccgacgccgg cttcgccggt gaggatcggg ttgttctggc ggcggcgcat
     1021 caggatgtcg atggcctggc ggatttcggc gtcgcggccg atcaccgggt cgaccttgcc
     1081 gtcgcgcgcg cgctgcgtca ggttggtggt gaactggtca agcgccggcg tcttgccggc
     1141 ctgcggcgcg ctgccctgca acgggtcgcc gccctcctcc tcggccggct tgccggacag
     1201 gccggtcact tcggcggctt cggcggaacc ttcggtgatt tcgtcgaact tgtgcttgag
     1261 ttcgtcgagc cggaccttgg ccagcagatg cgcgccgcgg gtcgccagtt gggcgagttc
     1321 aggttcggtg agcaaggcca gcaacaggtg cccagagcgg atgcgcggca ggcgggtgtc
     1381 gagcgaggcg atcagccagg cttgctcgaa cagcttgatg atgtgcgtgg aaaacaccgg
     1441 cgtgcgcgtg ttgccgttgc ggaagcggcc gacttcgtcg tgcagttcgc gctccaggcc
     1501 gctcgggctg atcttgcagc ggcgcaggat cgtcgtcagg tcgctgtcct gctgttcgag
     1561 cagggccagc aacaggtgct cgaaatcgac ttcgtaattg ccgcgcgcca tgcacaggcc
     1621 ggcagcgcgt tcggtggcct ggcggcaggt ggtgttgagt ttgctgatca gggttttcag
     1681 ggaggtgctc atcggtgctt ctttcgctgg gaatcgctaa tgaatggtct gggtcaggta
     1741 gcggcaatcg ctgcggtcgc gggtttcggg gcggctgatc aggaagctgt cgtagccgag
     1801 gcggccgccg ttcagggaga ccggcctgac gtccggggcg cgcaggatgg gacggatttc
     1861 gtattcgaac tggccgccgg tggccagctc gagcagcttt tccagggcat gggcgagttc
     1921 gccgcgcggc atgaagccca tgtactgctc gtgcgccatc ggcccgatat gcaggcggat
     1981 gcgcaggttg cgctgccaga cccgttcgcc gaccagggaa tcgacaccca gcgcgcagtt
     2041 ctggccacca agcgtggtcc attgagcgcg cggcacgacg taccagcgac caacgaactg
     2101 ttcgacacga accggggtcc ggaaataggc ggcaagcacc cgcgccatcg cttcgccgga
     2161 aaccggccgc tgccgcagca aggtggtgaa ataggcgatc gattcgtctt cgatggcgcc
     2221 cggcgcgtcg ttcagccggt cgcgcagggc atcgaagcca accccaccga gggccaggat
     2281 cagcggcagg aagcggttgc ggcgattggt ctcgtactgg atgggcaggc ggtatttttt
     2341 ccaggcgcgg tagaaatggc cgacggcacg gttggtgaac aggtctagga aagcgcgccc
     2401 cgaggtatcg cgcttgaaac gttcgtggtc gatgacctgc tcggtgtaat gaacgggcaa
     2461 ggtgccgttg acgccgagca t
//
LOCUS       DAOSDX010000414         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14843_length_2470_cov_3.272050, whole genome shotgun sequence.
ACCESSION   DAOSDX010000414 DAOSDX010000000
VERSION     DAOSDX010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14843_length_2470_cov_3.272050"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLN02_09380"
     CDS             complement(<1..497)
                     /locus_tag="PLN02_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOI52576.1"
                     /translation="MDTARDLYLSATLRRIEARHANLPLMQRAGLAAADFAGELADPR
                     NRPVLILAGPGNNGGDAFVVGSRLRERFFAVHLVFAGDPAALPADAAAAREAFLAGGG
                     STLGEIPEIDHWGLIVDGIFGIGLRRAPEGRYAEWIATANRLAQRDACPLLALDCPSG
                     LDADSG"
     gene            705..1973
                     /locus_tag="PLN02_09385"
     CDS             705..1973
                     /locus_tag="PLN02_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52577.1"
                     /translation="MRNLVDLSLPVEPIAPHHLIGTLGDIFLQERHERLLSLPIVNEG
                     RPVGVISRHNMQKIFMSRYGREIQGKKPVETIMNPAPLLVSVDQSMEEASHFVTRNIA
                     FPITEDFILIKDGQYHGIGSVIDLLRGMEERMAEQNASLSNALSLLKASQSQIIQSEK
                     MASLGQMVAGVAHEINTPLGYVKNNLEMASEAFKLLRDIGVAHNALLSALQTGEASDE
                     EVHQRLSELEARNAGFQAVYPEDEMDALFKDTLYGLEQISEIVVNLKNFSRLDQAAVD
                     NVNINQCIDSALLIARNVIKHKAEVERNYGSLPTVRCAPSQINQVILNLLTNAAHAID
                     RPDGRIVIRTQADERYVHIVLTDNGKGIPPEVIKKIFDPFFTTKPVGQGTGLGLSIAF
                     KIVQDHKGLIRVKSKPGLGSAFCVSLPRST"
     gene            1997..>2470
                     /locus_tag="PLN02_09390"
     CDS             1997..>2470
                     /locus_tag="PLN02_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52578.1"
                     /translation="MIAKSAPPCPLGKAGMLPADRPTILFVDDEERILRSLRMLFAAS
                     YRVLMTTNGHEALEILRKERVHVLVSDQRMPIMTGVELLRQAREYSPPSMRLLLTGYS
                     DMEAIIGSINEGEIFRYINKPWNAEDFRRTVGEAMEIAIKLWDPAPVEVPPPAPDE"
BASE COUNT          521 a          757 c          739 g          453 t
ORIGIN      
        1 ccgctgtcgg cgtcgaggcc ggacgggcag tcgagggcga gcagcgggca ggcatcgcgt
       61 tgcgccagcc ggttggcggt ggcgatccac tcggcgtagc ggccttccgg tgcgcgcctg
      121 agaccgatgc cgaagatgcc atcgacgatc agtccccaat gatcgatctc gggaatctca
      181 ccgagcgtgc tgccgccgcc ggccaggaaa gcctcgcgcg ccgccgccgc atcggccggc
      241 agggcggcgg gatcgccggc aaagacgaga tggacggcga agaagcgctc gcgcagccgg
      301 ctgccgacga cgaaggcgtc gccgccgttg ttgccggggc cggcgaggat gaggaccggg
      361 cggttgcgcg gatcggccag ttcgccggcg aaatcggcgg ccgccagccc ggcgcgctgc
      421 atcagcggca ggttggcgtg gcgggcttcg atccggcgca gcgtggcgct caggtagaga
      481 tcgcgggcgg tatccatggc aggattttag cgctacatgg cgcgggtgag gcggacctgt
      541 ccgctggctg acgttcagtg gccccatccc tggggctggc ggggcagttt tacaaaatgg
      601 ggtgagtagg tttgattgtc ccgcaagctc gttcctggac acctgattgc aatcttcgtg
      661 gttgaaaatc ggccaccgcc tctgacgcat ttgtgaattc ccgcatgaga aacctggtcg
      721 atctcagcct gcctgttgaa ccgattgcgc cgcatcacct gatcggcacc ctgggcgata
      781 tctttcttca ggagcgacat gaacgtctgc tgtcgcttcc catcgtgaat gaagggcggc
      841 ccgtcggcgt catcagccgg cacaacatgc agaaaatctt catgtcgagg tatggtcggg
      901 aaatccaggg gaaaaagccg gtcgaaacga tcatgaatcc cgcgcctttg ctggtttccg
      961 tcgatcagtc catggaagag gccagccact tcgtgacccg caacatcgcc ttccccatca
     1021 ctgaagattt catcctcatc aaggatgggc agtaccacgg cattggctcg gtcatcgacc
     1081 tgctgcgcgg gatggaagaa cgcatggcgg aacagaacgc ttcgctttca aacgcgctgt
     1141 ccctgttgaa agcctcgcaa tcgcagatca ttcagtcgga aaagatggcc tcgctcggcc
     1201 agatggtggc gggcgttgcc catgaaataa acacgccgct cggctacgtg aaaaacaatc
     1261 ttgaaatggc cagcgaggcg ttcaaactgc tgcgggacat tggcgtcgct cacaacgccc
     1321 tgctgtcggc attgcagaca ggcgaggctt cggacgaaga agtccaccag cggttatccg
     1381 aactcgaagc acgcaacgcc gggtttcaag ccgtctatcc ggaagatgaa atggatgccc
     1441 tgttcaagga caccctgtac ggcctggagc aaatctccga gatcgtcgtc aacctcaaga
     1501 atttcagccg cctggaccag gcagccgtag acaacgtcaa tatcaatcaa tgcatcgaca
     1561 gcgcgctcct catcgccagg aacgtcatca aacacaaggc cgaggtcgaa cgcaattatg
     1621 gaagcttgcc gacggtacgc tgtgctccgt cgcaaatcaa tcaggtcata ctgaatctgc
     1681 taaccaacgc cgcccacgca atcgaccggc ccgacggtcg tatcgtcatc cgtacccagg
     1741 cagacgaacg ctacgtccat atcgtgctga ccgataacgg caagggaatt cctccagaag
     1801 tcatcaagaa gattttcgac ccgttcttca ccaccaagcc ggttggccag gggaccggcc
     1861 tcggactgtc gatcgccttc aagatcgttc aggaccataa aggcctcatc cgcgtgaaat
     1921 cgaagccggg gctgggctcc gccttctgcg tcagtctccc tcgcagcacc tgaggtcgag
     1981 agaaaggaaa acgagtttga tcgccaaatc cgcaccacct tgtccgctcg gcaaagccgg
     2041 gatgctaccg gcggaccggc caaccatcct gtttgtcgat gacgaagagc gtatcctgcg
     2101 ctccctgcgc atgctgtttg ccgccagtta ccgggtcttg atgaccacca acggtcacga
     2161 agccctggaa atcctgcgca aggaacgcgt gcacgttctg gtcagcgacc agcgcatgcc
     2221 gatcatgacc ggcgtcgagt tgctgcgcca ggcgcgcgag tattctcctc ccagcatgcg
     2281 cctcctcctg accggctact ccgacatgga agcgatcatc ggttcgatca atgaggggga
     2341 gatattccgc tatatcaaca agccgtggaa tgcggaagat ttccgccgta ccgtcggcga
     2401 agcgatggaa attgccatca agctgtggga cccggcccct gtcgaagtgc caccacccgc
     2461 ccctgacgaa
//
LOCUS       DAOSDX010000415         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14902_length_2463_cov_3.497093, whole genome shotgun sequence.
ACCESSION   DAOSDX010000415 DAOSDX010000000
VERSION     DAOSDX010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14902_length_2463_cov_3.497093"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLN02_09395"
     CDS             complement(<1..220)
                     /locus_tag="PLN02_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52579.1"
                     /translation="MTDPIRHACRLAGLLASLCLPLAGHAAPQEPLTINFVGVDVESA
                     VKAVGQVTGRNFVIDPRVKGSINIVSNRP"
     gene            complement(213..698)
                     /locus_tag="PLN02_09400"
     CDS             complement(213..698)
                     /locus_tag="PLN02_09400"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOI52580.1"
                     /translation="MPSLFLSRRLPSPAALQRFAAPAATTLVALAASWLFGALAGDFL
                     LPASAPVHIAEHRPQPLAERLASRLAFAGNTPSTAAAPLNLTLAGISASADAGVARAL
                     IRVEGQPGLRLVAIGDEIGNGLRLRAIENDAVTLAGPAGDSRLTLPKPANLTPTTDPD
                     D"
     gene            807..1367
                     /locus_tag="PLN02_09405"
     CDS             807..1367
                     /locus_tag="PLN02_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOI52581.1"
                     /translation="MDNVNLTDNFLIAMPALEDPYFSNALVYICEHNENGALGIIVNR
                     PLDMNLAGLFEKIDIPLEQAGLARLPVYFGGPVQLDRGFVLHRPVGNWQSTLAINSEI
                     GLTSSRDILAAVGKDGVPAEILVTLGYSGWDAGQLEEELAQNSWLTVPAKSAILFDLP
                     PEERLPAAMQKLGVSFSQLSDVAGHA"
     gene            1369..1794
                     /gene="ruvX"
                     /locus_tag="PLN02_09410"
     CDS             1369..1794
                     /gene="ruvX"
                     /locus_tag="PLN02_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOI52582.1"
                     /translation="MGTVLAFDFGEKRIGVATGETLLASAHPLTVIRAESNDERFAAI
                     GKLIAEWQPERLVVGLPTHADGTPHEMTRLATKFAERLQKRFNLPVAMADERLSSLDA
                     EARLRETGRNAKSAKPLLDAVAAQLILQTWFESSSHASQ"
     gene            1781..2287
                     /gene="pyrR"
                     /locus_tag="PLN02_09415"
     CDS             1781..2287
                     /gene="pyrR"
                     /locus_tag="PLN02_09415"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOI52583.1"
                     /translation="MLANDPLPDAEAQCRQLAELIRPLLPKNPALVGIYSGGAWIAER
                     LRELLGLTEEIGLIDVSFYRDDFAEKGLHPQVKPTLIPFEVEGRHLILVDDVLYTGRT
                     TRAAINELFDYGRPAAVDLAVLADRGGRELPVAATWRVWDVALAPGQSLVLGRDERDQ
                     LAWRLENA"
     gene            2280..>2463
                     /gene="pyrB"
                     /locus_tag="PLN02_09420"
     CDS             2280..>2463
                     /gene="pyrB"
                     /locus_tag="PLN02_09420"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335698.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOI52584.1"
                     /translation="MHNPQLNANGELIHLLSTEGLPRSIMTQILDTAESFMEISSREV
                     KKVPLLRGKSVFNLFFE"
BASE COUNT          407 a          776 c          870 g          410 t
ORIGIN      
        1 ttgggcgatt cgaaacgatg ttgatgctgc ccttgacgcg cgggtcgatg acgaaattgc
       61 gtccggtcac ctggccgacc gctttcaccg ccgattcgac atcgacgccg acgaagttga
      121 tggtcagcgg ttcctgcggc gcggcgtggc cggccagcgg caggcagagg ctggccagca
      181 gcccggcgag gcggcaggcg tggcggatcg ggtcagtcat cggggtcggt ggtgggcgtg
      241 aggttggcgg gtttgggcag ggtcagccgg ctgtcgccgg cgggaccggc gagggtcacg
      301 gcatcgtttt cgatggcacg caggcgcagg ccgttgccga tttcgtcacc gatggcgacg
      361 aggcgcaggc cgggttgccc ttcgacgcgg atcagcgcgc gtgccacgcc ggcgtcggcg
      421 gaggcgctga tgccggcgag cgtcaggttg agcggcgctg ccgccgtgct gggcgtattg
      481 ccggcgaagg cgaggcggct ggccaggcgt tcggcgagcg gctgcgggcg gtgctcggcg
      541 atgtgcaccg gggcgctggc cggcagcagg aagtcgccag ccagcgcccc gaacagccag
      601 ctcgcggcga gcgccacgag cgtcgtcgcc gctggtgcgg cgaagcgctg cagggcggcc
      661 ggggacggca ggcgacggga aaggaaaagg ctgggcatgc cggatgtcgg tgggcggtga
      721 gtagggcaga ttaatgcagc gctgttaccg ttccgtgacg gcgatcggcg cggcagcagc
      781 cgggttttcc tttaaaattg ccgcccatgg acaacgtcaa cctgaccgac aacttcctga
      841 tcgccatgcc ggcgctggaa gacccctatt tctcgaatgc gctcgtctac atttgcgagc
      901 acaacgagaa cggcgcgctg ggcatcatcg tcaatcggcc gctcgacatg aacctggccg
      961 gtctgttcga gaagatcgac atcccgctcg agcaagcggg gctggccagg ctgccggtgt
     1021 atttcggcgg gccggtgcag ctcgaccgcg gtttcgtgct gcaccggccg gtcggcaact
     1081 ggcagtcgac gctggcgatc aacagcgaga tcgggctcac cagttcgcgc gacatcctcg
     1141 ctgccgtcgg caaggacggg gtgccggcgg aaatcctcgt caccctcggc tattccggtt
     1201 gggatgccgg ccagctcgag gaagagctgg cgcagaactc ctggctgacg gtgccggcca
     1261 agtccgccat cctgttcgac ctgccgccgg aagagcgtct gccggcagcg atgcagaaac
     1321 tgggcgtcag tttctcgcag ctttccgacg tcgccggtca cgcctgacgt ggggacggtg
     1381 ctcgctttcg atttcggcga aaagcggatc ggcgtcgcca ccggcgagac gctgctcgca
     1441 tcggcgcatc cgctgacggt gattcgcgcc gagtcgaacg acgagcgctt cgcggcgatc
     1501 ggcaagctga tcgccgaatg gcagccggag cggctggtcg tcggcctgcc gacgcacgct
     1561 gacggcacgc cgcacgaaat gacccgcttg gcgacgaagt tcgccgagcg cctgcaaaaa
     1621 cggttcaatc tgccggtcgc catggccgac gagcggctca gttcgctcga cgccgaggcg
     1681 cgcctgcgcg agaccggacg caatgccaag tcggcgaagc cgctgctcga tgcggtcgcc
     1741 gcccaattga ttctccaaac ctggttcgaa agttcgtcgc atgctagcca atgaccccct
     1801 gcccgatgcc gaagcccagt gccggcaact ggccgaactg atccgtccgc tgttgccgaa
     1861 gaatcccgcg ctggtcggca tctacagcgg cggcgcctgg atcgccgagc gcctgcgtga
     1921 attgctcggc ctgaccgagg aaatcggcct gatcgacgtt tccttctacc gcgacgattt
     1981 cgccgagaag ggcctgcacc cgcaggtcaa gccgacgctg attccgttcg aggtcgaagg
     2041 ccgccacttg atcctggtcg atgacgtgct ctataccggg cggacgacgc gggcggcgat
     2101 caacgaactg ttcgattacg ggcggccggc ggcggtcgat ctcgccgtcc tcgccgaccg
     2161 cggtgggcgc gagctgccgg tggcggcgac ctggcgggtc tgggacgtgg ccctggcgcc
     2221 cggccagagc ctggtcctgg gccgcgacga acgcgatcaa ctggcctgga ggctggaaaa
     2281 tgcataatcc gcagctcaat gccaacggcg aactgatcca cctgctgtcg accgaaggtc
     2341 tgccgcgttc gatcatgacg cagatcctcg ataccgccga atccttcatg gaaatctcca
     2401 gtcgcgaggt gaaaaaagtg ccgctgctgc gcggcaagag cgtgttcaac ctgttcttcg
     2461 aga
//
LOCUS       DAOSDX010000416         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14910_length_2462_cov_3.715413, whole genome shotgun sequence.
ACCESSION   DAOSDX010000416 DAOSDX010000000
VERSION     DAOSDX010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14910_length_2462_cov_3.715413"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="coaD"
                     /locus_tag="PLN02_09425"
     CDS             <1..357
                     /gene="coaD"
                     /locus_tag="PLN02_09425"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOI52585.1"
                     /translation="QPRFTLAERVEMAHEVVGDLPNIEIVGFNTLLMSFVHEREAKVI
                     VRGLRAVSDFEYEFQMAGMNRSLYPEVETVFLTPGEQYMFISATMVREIARLGGDVGK
                     FVHPSVEKRLRATTTA"
     gene            378..635
                     /locus_tag="PLN02_09430"
     CDS             378..635
                     /locus_tag="PLN02_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOI52586.1"
                     /translation="MSLIITDECINCDVCEPECPNEAISQGDEIYVIDPGKCTECVGH
                     YDEPQCVQVCPVDCIPKDPAHEETEDQLWAKYEKLTGNPRH"
     gene            complement(713..2350)
                     /locus_tag="PLN02_09435"
     CDS             complement(713..2350)
                     /locus_tag="PLN02_09435"
                     /inference="COORDINATES: protein motif:HMM:NF021493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadG family pilus assembly protein"
                     /protein_id="HOI52587.1"
                     /translation="MRALPRAARAQRGAVSVMAAVALTMVIGAALLAVDLGSLFNTRR
                     ELQTVADTAALSAVNNLAAAQAIALDAASLNHFPVPGDQQNTLGTVLGHYDVDAREFT
                     PGGSAAEYNAVQVTVTTQQPYFFMLGSRQVTASAVAMRNDIAALSVGTSLLDIDTEKS
                     VLLNALLGKLLNTSLNLSAIAYQGLVNTSIRLLDLVKVVGSVGTVEELLKLDLTVGEL
                     LELSARALERSSILGLDATVVQTLDLLALRADGNLNLKLADLINVDLAPGNQAAEAKI
                     NLMQLVTLAAQVANREHFIDIPVLGINLPGLVKLDAALTMIESPGIAIGPAGQDASGN
                     WRTSVHTAQWRLKLDLLVGELLGGLVRLPLYLEIGAGEAWLKDIDCRYPRDASIVQVG
                     AASSAVRAYVGNVNPDAMTNRSVAATVTPATILNVLGLVKATARLQVDLPGASGDLEF
                     TGPFDGSNTQRIHGLTTAGLGEILLSGMGAVLDVELLGIKLGLGEVVEALVTLLAPVF
                     ALLDSLIAPVLSLLGIQIGIADVTTFDLSCGAPQLVR"
BASE COUNT          430 a          779 c          848 g          405 t
ORIGIN      
        1 cagccgcgct tcacgctggc cgaacgcgtc gagatggcgc acgaggtagt cggcgatctg
       61 cccaacatcg agatcgtcgg cttcaacacg ctgctcatga gcttcgtcca tgagcgcgag
      121 gcgaaggtca tcgtgcgcgg gctgcgcgcc gtgtccgact tcgaatacga attccagatg
      181 gccggaatga accgcagcct ctaccccgag gtcgaaacgg tattcctcac ccccggcgag
      241 caatacatgt tcatttccgc aacgatggta agggaaattg cgcgactggg cggtgatgta
      301 ggaaaattcg tccatccttc agtagaaaag cgcctgcggg caacaaccac tgcttaaacg
      361 agagaggaac aactgacatg tccctgatta ttacggacga gtgcatcaac tgcgacgtgt
      421 gcgaaccgga atgcccgaac gaggcgattt cccagggtga cgaaatctac gtcatcgatc
      481 cgggcaagtg cacggagtgc gtcggtcact acgacgaacc gcaatgcgtg caggtctgcc
      541 cggtcgattg catccccaag gacccggccc acgaggaaac cgaggaccag ctctgggcca
      601 agtacgagaa gctgaccggc aatccccgcc actgagcgct agcggcgccg ctccggcgcc
      661 ccacgaaaaa cgcccgccac cgtcgcgggc gttttctttt gtggctggcg gttcagcgca
      721 ccagttgcgg ggcgccgcag gaaaggtcga aggtggtgac gtcggcgatg ccgatctgga
      781 tgccgagcag cgacaggacc ggcgcgatca ggctgtcgag cagcgcgaag accggcgcca
      841 acagcgtcac cagcgcctcg accacctcgc ccaggccgag cttgatgccg agcagttcga
      901 cgtcgagcac cgcccccatg ccggagagca ggatctcacc caggccggcg gtcgtcagcc
      961 cgtggatgcg ctgcgtgtta ctgccgtcga acgggccggt gaattcgagg tcgccgctgg
     1021 cgccgggcag gtcgacctgg agccgtgccg tcgccttgac caggcccaac acgttgagga
     1081 tggtcgcagg ggtcaccgtc gccgccaccg agcggttggt catcgcgtcg ggattgacgt
     1141 tgccgacgta ggcgcgcacc gcgctcgacg cggcgccgac ctggacgatg ctggcgtcgc
     1201 gcggatagcg gcagtcgatg tccttgagcc aggcctcgcc ggcgccgatt tccaggtaga
     1261 gcggcagacg gaccaggccg ccgagcagtt cgccgaccag caggtcgagc ttgagccgcc
     1321 attgcgcggt atgcaccgag gtccgccagt tgccgctggc gtcctgaccg gccgggccga
     1381 tggcgatgcc gggcgattcg atcatggtca gcgcggcgtc gagcttgacc agccccggca
     1441 ggttgatgcc gagcaccggg atgtcgatga aatgttcgcg gttggccacc tgcgccgcca
     1501 gcgtcaccag ttgcatcagg ttgatcttgg cttccgccgc ctggttgccg ggcgccaggt
     1561 cgacgttgat caggtcggcg agcttcaggt tgaggttgcc gtcggcgcgc agggccagca
     1621 ggtcgagcgt ctgcacgacg gtggcgtcga ggccgaggat gctcgagcgt tcgagcgcgc
     1681 gcgccgacag ttcgagcagt tcgccgacgg tcaggtcgag cttgagcagc tcctcgacgg
     1741 tgccgaccga accgaccacc ttgaccaggt cgagcaggcg gatcgaagta ttgaccagcc
     1801 cctggtaggc gatcgccgac aggttgagcg aggtgttgag cagcttgccg agcagcgcgt
     1861 tgagcagcac cgatttctcg gtatcgatgt cgagcagcga ggtgccgacc gacagcgcgg
     1921 cgatgtcgtt gcgcatggcg acggcactgg ccgtcacctg ccggctgccg agcatgaaga
     1981 aatacggctg ctgcgtcgtc accgtcacct gcaccgcgtt gtattcggcg gcgctgccgc
     2041 ccggcgtaaa ctcgcgggca tcgacgtcgt agtggccgag caccgtgccg agcgtgttct
     2101 gctggtcacc ggggaccggg aagtggttga gactggcggc gtcgagggcg atcgcctgcg
     2161 ccgcggccag gttattgacc gccgacaggg cggcggtgtc ggccaccgtc tgcagctcgc
     2221 gccgggtatt gaagagactg ccgagatcga ccgccagcag ggcggcaccg atcaccatgg
     2281 tcagcgccac ggcggccatg acgctgaccg cgccgcgctg ggcgcgcgct gcccggggca
     2341 gtgctctcat cggtagtctc cgctgccggt tcagcgggcg ttgccgaccg cggcggcagt
     2401 cgccggctgg tcgatctggc cgaccgtggt cggcttcttg aaactgtcga ggtaacgctg
     2461 gt
//
LOCUS       DAOSDX010000417         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14951_length_2457_cov_2.829309, whole genome shotgun sequence.
ACCESSION   DAOSDX010000417 DAOSDX010000000
VERSION     DAOSDX010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14951_length_2457_cov_2.829309"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLN02_09440"
     CDS             complement(<1..357)
                     /locus_tag="PLN02_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52588.1"
                     /translation="MRRLGAFLLLLTILTGYCAANPYYDAEKAHHRPDGFVNSDPAAR
                     IDGAPWYEVLGRRWRGDFQPAAEPAGGYAAFAARWVVQPDRAQLDDPGGLPRLTWLGH
                     ATLLVQAGGRNILIDPN"
     gene            complement(362..1300)
                     /locus_tag="PLN02_09445"
     CDS             complement(362..1300)
                     /locus_tag="PLN02_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HOI52589.1"
                     /translation="MPPENAILTISLFAAFADGSNDDREREHIRRFAESMGDTAPELP
                     RLYQDVLFKRVTLDGAVQALGELGQRQFAYEMAVCVCDADGVQTAAEQAFLGNLKTRL
                     GLDGDAGSMATATQANNLAAAAAAPLATTAAAAQPTVNDAELDKSILNYSILNGALEL
                     LPQSWASVAIIPLQVKMVYRIGKAYGVELDQGHIKEFLATVGVGLTSQYLEQFGRKLL
                     GGLLGKMAGKTAGKIGRAATGMAFSFATTYALGQVAKRYYASGRQMSTQLLQESFQGL
                     LGPAKQLQSQYLPQIEQRAQTLDAGQVMNLVRGGGL"
     gene            complement(1389..1850)
                     /locus_tag="PLN02_09450"
     CDS             complement(1389..1850)
                     /locus_tag="PLN02_09450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOI52590.1"
                     /translation="MLAFQDYYPENVAHCYGCGKLNEHGHQIKTYWDGDETVTRYTPR
                     PEHTAIPGFVYGGLLASLIDCHGTGTAAAAMYRQENRAMDSEPPFRFVTASLHVDYLK
                     PTPLGPELEIRGKVKEIRGRKVIVEATVYADGVATARGEIVAAQMPPSFTA"
     gene            2022..>2457
                     /locus_tag="PLN02_09455"
     CDS             2022..>2457
                     /locus_tag="PLN02_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOI52591.1"
                     /translation="MANDSIVDRLRSLIVPGQRRSSLTAKGLERLRAQLGECAAGQGG
                     EVSARTRAAKLAETYLNLDDAGRQQFLKMIALEFGPAPDKVAAAHAAYQAAVGTPGQW
                     KAEAALRAAMRSSRIRIMTQFNAIPQGVKFLVDLRADLLRYLH"
BASE COUNT          372 a          734 c          904 g          447 t
ORIGIN      
        1 gttggggtcg atcaggatgt tgcggccgcc ggcctgcacc agcagcgttg cgtgaccaag
       61 ccaggtgagg cggggcaggc cgccgggatc gtcgagctgg gcgcggtcag gctgtaccac
      121 ccagcgcgcg gcgaaggccg cgtagccgcc ggcgggttcg gcggccggct ggaaatcgcc
      181 gcgccagcgt cggcccagca cttcgtacca gggcgcgccg tcgatgcggg ccgccgggtc
      241 gctgttgacg aagccgtccg gccggtgatg cgctttttcc gcgtcgtagt aagggttggc
      301 ggcgcagtac ccggtgagga tggtgagcag caggaggaag gcgccgaggc ggcgcatcgc
      361 gtcagagacc gccgccgcgc accaggttca tcacctgccc ggcgtcgagt gtctgcgccc
      421 gctgctcgat ctgcggcagg tactggctct gcaattgttt ggccgggccg agcaggccct
      481 ggaagctttc ctgcagcaac tgcgtgctca tttgccggcc gcttgcgtaa taacgcttgg
      541 cgacctggcc gagggcgtag gtggtggcga acgagaaggc catgccggtt gccgcgcgac
      601 cgatcttgcc cgcggtcttg ccggccatct tgccgagcag gccgccgagc agcttgcggc
      661 cgaattgttc gaggtactgc gaggtcagac cgacgccgac ggtggcgagg aattccttga
      721 tgtgtccctg atcgagttcg acgccgtagg ctttgccgat gcggtaaacc atcttcacct
      781 gcagcgggat gatcgccacc gaggcccagg attgcggcaa cagttcgagg gcgccgttga
      841 ggatcgagta gttgaggatg gatttgtcga gttcggcgtc gttgaccgtc ggctgggccg
      901 cggccgccgt tgtggcaagc ggggcggcgg cagcggcggc caggttgttg gcctgggtgg
      961 cggtggccat gctgccagcg tcgccgtcga ggccgagacg ggttttcaga ttgccgagga
     1021 aggcctgttc ggcagccgtc tgcacgccgt cggcatcgca gacgcagacc gccatttcgt
     1081 aggcgaactg gcgctgaccg agttcgccca gcgcctgcac ggcgccgtcc agcgttaccc
     1141 gcttgaacag cacgtcctgg tagagccggg gcagttcggg ggcggtgtcg cccatcgatt
     1201 cggcgaagcg gcggatgtgt tcgcgttcgc ggtcgtcatt gctgccgtcg gcgaaagcgg
     1261 cgaacaggct gatcgtcagg atggcgtttt ccgggggcat ggcacggtct cgtcgttgcg
     1321 gaacgacgca ttttgcctac ggccccggat tttttcagtg gccgtctgta acggtgtgtt
     1381 gccgcagatc aggcggtgaa gctgggcggc atctgggcgg cgacgatttc gccgcgcgcg
     1441 gtggcgacgc cgtcggcgta gacggtggct tcgacgatca ccttgcggcc gcggatttcc
     1501 ttgaccttgc cgcggatttc cagttccggt ccgagcggcg tcggcttcag gtagtcgacg
     1561 tgcagcgagg cggtgacgaa gcggaacggc ggttcgctgt ccatcgcccg attttcctgg
     1621 cggtacatcg cggcggcggc ggtgccggtg ccgtggcagt cgatcagcga ggcgagcagg
     1681 ccgccataga cgaaaccggg gatcgccgtg tgttccgggc gcggcgtgta gcgggtgacg
     1741 gtttcgtcgc cgtcccagta ggtcttgatc tgatggccgt gttcgttcag cttgccgcaa
     1801 ccgtagcagt gggcgacgtt ttcggggtag tagtcctgga aggcgagcat gtgtatctcc
     1861 gcgtggggtt gatcaggaaa cgatgatagt gccgcggcat gggcaaggcg attgcatggc
     1921 cggacagctt gcatgcaaat tccgccagag ccgcggcggc tcctgaagac gctgacgggg
     1981 cggggcttct cgggcaaaat gccgggaagg aggaaagcac catggctaat gacagcatcg
     2041 ttgatcgctt gcgctcactg atcgttcccg ggcaacgccg cagctcgctg accgcgaagg
     2101 ggctcgaacg cctgcgcgca caactgggtg aatgtgccgc cgggcaaggc ggtgaggttt
     2161 cggcgcggac gcgcgcggcg aagctggcgg aaacctatct gaaccttgac gatgctggcc
     2221 gccagcagtt cctgaaaatg atcgctctcg aattcggtcc ggcgccggac aaggtcgccg
     2281 ctgcccacgc cgcctaccag gcggcggtcg gcacgcccgg ccagtggaag gcggaagcgg
     2341 cgctgcgcgc ggcgatgcgc tcgtcgcgca tccgcatcat gacgcagttc aacgcgatac
     2401 cgcagggcgt caagtttctc gtcgacctgc gcgccgacct gctgcgctac ctgcacg
//
LOCUS       DAOSDX010000418         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_14959_length_2456_cov_3.687630, whole genome shotgun sequence.
ACCESSION   DAOSDX010000418 DAOSDX010000000
VERSION     DAOSDX010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14959_length_2456_cov_3.687630"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLN02_09460"
     CDS             complement(<1..983)
                     /locus_tag="PLN02_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOI52592.1"
                     /translation="MPDSAAQLPTPLPRPHRLLGLAALLVAFLALALFSYRLSEHYGI
                     AEMREAASHQLDILGAAIDSEVTRHASIPSAVELNADIVALLRGPSEGQDILQAEANS
                     FLQKLNDHLGGPAIFVLDTSGTVVASSDWIFSDNLLGSDLSYMPFFRDAVRGTPARHY
                     AVDHIRHEPGYYFALPIRDEAQEWKVIGVAVVKSGIREVERRWLAQEAPALIVDRNDV
                     VLLASPPEWRYATLRPQARDVLNRLGRQQFAGQPLGEISLDIDLAGADEGRIVRLPKH
                     LRDDVAPAQGARPYLAMSRHLSGTAWRIVVFSDLRPVGVQAATHAALSVAAL"
     gene            complement(1085..>2456)
                     /locus_tag="PLN02_09465"
     CDS             complement(1085..>2456)
                     /locus_tag="PLN02_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOI52593.1"
                     /translation="GDAEVPAGAYWGVHTLRAIENYPITGKTIGSYGELVRALALTKQ
                     AAAMANAELGQLDRTRADAIIAACQEIAGGKLHGEFVVDVIQGGAGTSTNMNANEVIA
                     NRALEILGHRKGDYAHLHPLNHVNMSQSTNDVYPTALRVAACFAIRGLLSAMAGLREA
                     FAAKAEEFKDVLKIGRTQLQDAVPMTLGQEFSTYAVMLHEDELRLGEAIALIREINLG
                     ATAIGTGINTDMRYSRLAIDHLSQLSGFALEAAPNLIEATQDCGAFVQLSGVLKRVAA
                     KLSKTCNDLRLLSSGPQAGLNEIRLPPRAAGSSIMPGKVNPVIPEVVNQIAFEVIGND
                     VTITMAAEGGQLQLNAFEPIIAHSLFKSIEHLAAGCRTLTAHCVRGITANRELLAERV
                     RTSAGLATALNPHIGYENATRVAQEALRSGRGVAELVEEMGLMDRAQLAAVLAPEVLT
                     APRRPG"
BASE COUNT          368 a          781 c          918 g          389 t
ORIGIN      
        1 agggcagcga cgctcagcgc ggcatgcgtc gccgcctgga cgccgaccgg ccgcaggtcg
       61 gaaaagacga cgatgcgcca ggcggtgccg gagaggtggc gcgacatcgc caggtagggg
      121 cgcgcgccct gcgccggcgc gacgtcgtcg cgcaggtgct tgggcaggcg gacgatgcgt
      181 ccttcgtcgg caccggcgag atcgatgtcg aggctgatct cgcccagcgg ctggccggcg
      241 aactgttgcc ggccgagacg gttgaggacg tcgcgggcct gcgggcgcaa ggtcgcgtag
      301 cgccattccg gcggcgaagc gagcagcacg acgtcgttgc ggtcgacgat cagcgccggc
      361 gcctcctgcg ccagccagcg ccgctcgacc tcgcggatgc cggacttgac gacggcgacg
      421 ccgatcacct tccattcctg cgcttcgtcg cggatcggca gcgcgaagta gtagcccggc
      481 tcgtgccgga tgtggtcaac cgcgtagtgg cgagccggcg tgccgcgcac cgcatcgcgg
      541 aagaagggca tgtaggagag gtccgagccg agcaggttgt cggagaatat ccagtcgctg
      601 gaggcgacga cggtgccgct ggtgtcgagg acgaagatcg ccggcccgcc gagatgatcg
      661 ttgagcttct gcaggaagct gttggcctcg gcctgcagga tgtcctgccc ctcgctcggg
      721 ccgcgcagca gggcgacgat gtcggcgttg agttcgacgg cgctgggaat cgaggcgtgg
      781 cgggtgacct cgctgtcgat cgcggcgccg aggatgtcga gctggtgcga ggccgcctcg
      841 cgcatttcgg caatcccgta atgctcggaa agccggtagg aaaacagggc cagcgccagg
      901 aaagcgacca gcagcgccgc cagtccgagc agacggtgcg ggcggggcaa gggcgtgggc
      961 agttgggcgg cggaatccgg catgacggga cgggctcagg gaatgggcat tgtacggctg
     1021 aacacccgcc cgccgcggcg ggcggcgtga cgggcagtag cacatccggt tgccggcatc
     1081 gacctcagcc cggccggcgc ggcgcggtca gcacttcggg cgccaggacg gcggccagtt
     1141 gtgcgcggtc catcagaccc atctcctcga ccagctcggc gacgccgcga ccgctgcgca
     1201 gcgcctcctg ggcgacccgg gtggcgttct cgtagccgat gtgcgggttc agcgcggtgg
     1261 ccagaccggc cgaagtgcgt actcgctcgg ccagcagctc gcggttggcg gtgatgccgc
     1321 gcacgcaatg ggcggtcagc gtgcggcagc cggcggccag gtgttcgatg ctcttgaaca
     1381 ggctgtgggc gatgatcggt tcgaaagcgt tcaactgcag ttggccgccc tccgccgcca
     1441 tggtgatggt gacgtcgttg ccgatcacct cgaaggcaat ctggttgacc acttccggga
     1501 tcaccggatt gaccttgccc ggcatgatcg acgaaccggc ggcgcgcggc ggcaggcgga
     1561 tctcgttgag cccggcctgc ggaccggaag agagcaggcg caggtcgttg caggtcttcg
     1621 acagcttggc ggcgacgcgc ttgagaacgc cggatagctg gacgaaggcg ccgcagtcct
     1681 gggtcgcctc gatcaggttg ggcgccgctt ccagcgcgaa accggagagc tgcgacaggt
     1741 ggtcgatggc cagcctggaa tagcgcatgt cggtgttgat gccggtaccg atcgccgtcg
     1801 ccccgaggtt gatctcgcgg atcagggcga tcgcctcgcc caggcgcagc tcgtcctcgt
     1861 gcagcatcac cgcgtaggtc gagaactcct ggccgagcgt catcggcacc gcgtcctgca
     1921 actgggtgcg gccgatcttc agcacgtcct tgaactcctc ggccttggcg gcgaaggctt
     1981 cgcgcagccc ggccatcgcc gacagcaggc cgcggatggc gaagcaggcg gcgacgcgca
     2041 gcgccgtcgg atagacgtcg ttggtgctct gcgacatgtt gacgtggttg agcgggtgca
     2101 ggtgggcgta gtcgcccttc cggtggccga ggatttccag cgcccggttg gcgatcacct
     2161 cgttggcgtt catgttggtc gaggtgccgg cgccgccctg gatgacgtcg acgacgaact
     2221 cgccgtgcag tttgccgccg gcgatttcct ggcaggcggc gatgatcgcg tcggcgcgcg
     2281 tgcggtcgag ctggccaagc tcggcattgg ccatcgccgc cgcctgcttg gtcagcgcca
     2341 gggcgcgcac cagttcgcca taactgccga tggtcttgcc ggtgatcgga tagttctcga
     2401 tcgcccgcag ggtatgcacg ccccagtagg cgccggccgg cacctcggcg tcgccg
//
LOCUS       DAOSDX010000419         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15038_length_2445_cov_2.730962, whole genome shotgun sequence.
ACCESSION   DAOSDX010000419 DAOSDX010000000
VERSION     DAOSDX010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_2445_cov_2.730962"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLN02_09470"
     CDS             complement(<1..786)
                     /locus_tag="PLN02_09470"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOI52594.1"
                     /translation="MPKVRSLFLSDIHLGTRACQADRLLDFLREYPADNTFLIGDIVD
                     FWAMSRSICWTRAQNTVVQKLLRRARSGDRVVFIPGNHDEVLREYCGIVFGDIEVADE
                     MEHETASGKRYLLIHGDQFDQVTRHHRWVAVLGDKAYDLLVSLNHWLSWVRRKLGLAG
                     YWSLAGYAKRRVKTALNFIFDFETSAVHHARERGLDGVICGHIHWATIRQIDGLDYIN
                     CGDWVDSCTAIVEHLDGRLELVTWTASPQAPRQLADRIGVRGRK"
     gene            916..>2445
                     /locus_tag="PLN02_09475"
     CDS             916..>2445
                     /locus_tag="PLN02_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52595.1"
                     /translation="MKISHRLVLLLAFTMSALLALGLVALTQFQRNNTMIRELTEGAI
                     PGFLASIELGAELKGLQIVAINLAHASERNVAGQLQEQLNAGKTKLGDALAAQRGLAE
                     GDAQRGLLDQAEESLKNYYAAIDDVARFSLAGQPLLAEATLSGNAAPYLAELEGVLET
                     LRVEKRRAKDESVARLENGLQETLLQLGAAFAAILAVLAGLGLALYRRIARPLREMER
                     TMQTIADSLDFTHRVPVINDDEIGRSIQAFNRLLDTLQQSLGEMAEVIRQNGVAAADM
                     QRSATTLAELAANSNGSSREIQEAIQQVQAQIDRIHADTGHAGELTAHSGKQAEDNGA
                     TIRQTVERIHDLAGNVETAAARVYALAAAGQNISGLVREIREIADQTNLLALNAAIEA
                     ARAGESGRGFAVVADEVRKLAERVSAATLSIAGQVEEIAVTSRQSTGLMQQVVEDIKL
                     NIDLASTAGNAMGDIERSAREVIETVGKIAEEVGVGHASSRQIVRQVDAIEQLMSHAN
                     VT"
BASE COUNT          439 a          839 c          806 g          361 t
ORIGIN      
        1 ttttcggcct cgaacgccga tgcgatccgc cagttgccgc ggcgcttgcg gcgacgcggt
       61 ccaggtgacg agttccaggc gaccgtcgag atgttcgacg atggcggtgc aggaatcgac
      121 ccagtcgccg cagttgatgt agtcgagacc gtcgatctgg cgtatcgtcg cccagtggat
      181 gtggccgcag atgacgccat ccagcccccg ctcgcgggcg tgatggacgg ccgaggtctc
      241 gaaatcgaag atgaagttga gcgccgtctt gacccgccgc ttggcgtagc cggcgagcga
      301 ccagtaaccg gccaggccga gcttgcgacg gacccaggac agccagtggt tgaggctgac
      361 caacaggtcg taggccttgt cgccgagtac cgcgacccag cggtggtggc gcgtcacctg
      421 atcgaactgg tcgccgtgga tgagcagata gcgcttgccg ctcgccgttt cgtgctccat
      481 ctcgtcggcc acttcgatgt cgccgaagac gatgccgcag tattcgcgca gcacttcgtc
      541 gtggttgccg ggaatgaaca ccacgcggtc gccggaacgc gcccggcgca gcagcttctg
      601 gaccacggtg ttctgcgccc gggtccagca gatgctgcgg ctcatggccc agaaatcgac
      661 gatgtcgccg atcaggaagg tattgtcggc cgggtattcg cgcaggaaat cgaggaggcg
      721 gtccgcctgg cacgcccggg tgccgaggtg gatgtccgat agaaacaggc ttctgacttt
      781 tggcatgggg gcatcatcgc cgccctccgt gaagcgcctg tgaccgccgc gtgacagcct
      841 gatgacaagg cgccggtcac cgtgctgcaa caatgttggc ttaggctgcc gcggtttttc
      901 cccgaaagct cggccatgaa gatatcgcac cgcctcgtcc tgctcctcgc gttcaccatg
      961 agcgcgctgc tcgccctcgg cctcgtcgcg ctgacccagt tccagcgcaa caacacgatg
     1021 atccgcgagc tgaccgaggg cgccatcccc ggctttctcg cgtcgatcga actgggcgcc
     1081 gagctgaagg gcctgcagat cgtcgccatc aacctggccc acgccagcga gcgcaacgtc
     1141 gccgggcagt tgcaggagca gttgaacgcc ggcaagacga aactgggcga cgccctcgcc
     1201 gcccagcgcg ggctcgccga gggcgacgcg cagcgcggcc tgctcgacca ggcagaggag
     1261 agcctgaaga actattacgc cgccatcgac gatgtcgccc gcttcagcct ggccggccaa
     1321 cccctgctcg ccgaagcgac gctgtccggc aatgcggcgc cctacctggc cgaactggaa
     1381 ggcgtcctgg agaccctgcg cgtcgagaag cggcgggcca aggacgagag cgtcgcccgc
     1441 ctcgaaaacg gcctgcagga aacgctgctc cagctcggcg ccgccttcgc cgccatcctg
     1501 gcggtcctgg ccgggctcgg cctcgccctc taccggcgca ttgcccggcc gctccgcgag
     1561 atggagcgga ccatgcagac gatcgccgac agcctcgatt tcacgcaccg cgtgccggtc
     1621 atcaacgacg acgagatcgg ccgttcgatc caggccttca accgcctgct cgacaccctg
     1681 cagcagagtc tcggcgagat ggccgaggtg atccgccaga acggcgtcgc ggccgccgac
     1741 atgcaacgtt cggcgacgac gctggccgaa ctcgcggcca acagcaacgg ctcgtcgcgc
     1801 gagatccagg aagcgatcca gcaggtgcag gcgcagatcg accgcatcca cgccgatacc
     1861 ggtcatgccg gcgaactgac cgcgcattcc ggcaagcagg cggaggacaa cggggcaacc
     1921 atccgccaga cggtcgagcg catccacgac ctcgccggca acgtcgagac cgccgccgcc
     1981 cgcgtctatg cgctggctgc ggcaggccag aacatttccg gcctggtccg cgaaatccgc
     2041 gagatcgccg accagaccaa cctgctggcg ctcaacgcgg caatcgaggc agcgcgcgcc
     2101 ggcgaatccg ggcgcggttt cgcggtggtc gccgacgagg tgcgcaagct ggccgaacgg
     2161 gtcagcgccg ccaccctgtc gatcgccggc caggtcgagg agatcgccgt cacctcgcgc
     2221 cagtcgaccg ggctgatgca gcaggtggtg gaagacatca agctcaacat cgacctcgcc
     2281 agcaccgccg gcaacgcgat gggcgacatc gagcgctcgg cgcgggaagt gatcgagacc
     2341 gtcggcaaga tcgccgagga ggtcggcgtc ggccatgcgt cgagccggca gatcgtccgc
     2401 caggtggacg ccatcgagca actgatgagc cacgccaacg tcacc
//
LOCUS       DAOSDX010000420         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15062_length_2443_cov_2.353434, whole genome shotgun sequence.
ACCESSION   DAOSDX010000420 DAOSDX010000000
VERSION     DAOSDX010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15062_length_2443_cov_2.353434"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLN02_09480"
     CDS             <1..205
                     /locus_tag="PLN02_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOI52596.1"
                     /translation="QNVPEDQLEPLIAIACPNVLFPYARETVSDAVTRAGFQAILLQP
                     VNFEAMYVQRLQEMQAAAEAQIQ"
     gene            214..651
                     /locus_tag="PLN02_09485"
     CDS             214..651
                     /locus_tag="PLN02_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOI52597.1"
                     /translation="MRRLLTAFCCLATALPALALDFRSVVVPAAVLYDAPSLKAKKLY
                     LIRAQTPVEVVVRLEGWVKVRDAEGGLAWIEASQVGERRTVIVTAADTELREAAADNA
                     PVSASLAKWLVLDYVGTPAPGWVAVKHRDGLSGVVRATQVWGL"
     gene            648..1637
                     /locus_tag="PLN02_09490"
     CDS             648..1637
                     /locus_tag="PLN02_09490"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOI52598.1"
                     /translation="MRVSLLGGGAWGTALAIAFAEAHAVTLWSRESDVAAELLSCREN
                     RRFLPGYRLPASVAVATDFAAAVGDAELLIVATPIAGLRPTAEKLRELPCTVPLLWVC
                     KGFEAGSGKLPHQVVADVLGPAALCGALSGPSFAEEVAAGQPTAITLASRDAAFARET
                     ARQLHGGSLRIYANDDLVGVEVGGAVKNVLAIATGVCDGLGLGLNSRAALMTRGLAEI
                     ARLGVALGAQRETFMGLAGMGDLILTCTGDLSRNRRVGLGLAQGKSLAEALDELGHVA
                     EGVYTARETARLAAELGVEMPISTAVAAVLDGRLTAPAAVEQLMARDPKEEIA"
     gene            complement(1649..2113)
                     /gene="trmL"
                     /locus_tag="PLN02_09495"
     CDS             complement(1649..2113)
                     /gene="trmL"
                     /locus_tag="PLN02_09495"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOI52599.1"
                     /translation="MFAVVLVHPEIPPNTGNIIRMCANTGAELHLVEPLGFDLSDKHL
                     RRAGLDYHEYARLVRHPDWPSCLAALAPRRMFAMTTRGSRNLYSVTMEADDVFVFGSE
                     TAGLPPAIRDSFPEERRLRLPMRAGQRSLNLSNAAAVTVFEAWRQCGFSDAV"
     gene            complement(2157..>2443)
                     /locus_tag="PLN02_09500"
     CDS             complement(2157..>2443)
                     /locus_tag="PLN02_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOI52600.1"
                     /translation="EIARTIAGLRQLPLARDLLQRQRATASQAGLDRKARQRNVRGAF
                     ACHADCSGQRLLLVDDVLTSGATADEAARSLIEHGALEVHVAVVARTLNS"
BASE COUNT          367 a          769 c          876 g          431 t
ORIGIN      
        1 ccagaacgtc cccgaagacc agcttgagcc gctgatcgcc atcgcctgcc cgaacgtgct
       61 ttttccctat gcccgggaaa cggtctcgga cgcggtgacc cgcgccggct tccaggccat
      121 cctgctgcag ccggtcaatt tcgaggcgat gtacgtgcag cgtctgcagg aaatgcaggc
      181 cgcggctgaa gcccagatcc agtaagcgcc gtcatgcgcc gcctgctgac agccttctgc
      241 tgcctcgcca cggccttgcc ggccctggcg ctggatttcc gttcggtcgt cgtgccggcg
      301 gcggtgctct atgatgcgcc ttcgctcaag gccaagaagc tctacctgat ccgcgcccag
      361 acgccggtcg aggtggtcgt ccgcctcgag ggctgggtca aggttcgtga cgccgagggc
      421 ggcctggcct ggatcgaagc cagtcaggtg ggcgaaaggc gcaccgtcat cgtcaccgct
      481 gccgacaccg agcttcggga ggcagcggca gacaacgcgc cggtcagcgc cagtttggcc
      541 aaatggctgg tgcttgacta cgtcggtacc ccagcgcccg gctgggttgc tgtcaagcat
      601 cgcgatgggc tgagcggcgt ggtccgcgcg acgcaggtct gggggctatg agagtttccc
      661 tgcttggggg cggtgcctgg ggcaccgcgc tggccatcgc tttcgccgag gcgcacgcgg
      721 tgactttgtg gtcacgcgaa agcgatgttg ccgccgaact cctttcctgc cgtgaaaacc
      781 gccgcttcct gccgggctac cgtttgccgg cgtcagtcgc ggtggcgacc gattttgccg
      841 cggcggttgg tgatgcagaa ttgctgatcg tcgcgacgcc gattgccggt ctgcggccga
      901 cggcggaaaa attgcgcgaa ttgccctgca cagtgccgct actctgggta tgcaagggtt
      961 tcgaagccgg tagcggcaag ttgccgcacc aggtggtggc agacgtgctt ggccctgccg
     1021 cgctttgcgg cgccctgtcc gggccgagct tcgccgagga agtcgccgcc ggacagccga
     1081 cggcgataac gctggcgtcc cgcgatgccg cctttgcgcg cgagaccgcg cgacagttgc
     1141 acggcggcag tctgcgcatc tacgccaacg acgatctggt cggtgtcgag gtcggcggcg
     1201 cggtcaagaa cgtgctggcg atcgccaccg gcgtctgcga cggcctcggc ctcggtctca
     1261 attcgcgggc cgcgctgatg acccgtggcc ttgccgaaat tgcccgtctc ggcgttgcac
     1321 tgggcgccca gcgcgagact ttcatgggct tggccggcat gggcgacctg attctgacct
     1381 gcaccggcga tctctcgcgc aaccgtcggg tcggcctcgg gctggcgcag ggtaagtcgc
     1441 tggcagaggc gctcgacgaa ctcggccatg tcgccgaagg ggtctatacc gcccgcgaga
     1501 cggcgcgtct ggcggccgaa ctcggtgtcg agatgccgat cagtacggcg gtggccgccg
     1561 ttcttgatgg tcgcttgacc gcgccggctg cggtcgagca actgatggcg cgcgatccca
     1621 aggaagagat cgcctgattt gaggtcgctc acacggcgtc gctgaagccg cactgtcgcc
     1681 acgcctcgaa gacggtgacg gcggcggcgt tcgacaggtt gaggctgcgc tgcccggcgc
     1741 gcattggcaa gcgcaggcgg cgctcttccg ggaagctgtc gcggatcgct ggcggcaggc
     1801 cggcggtttc gctgccgaag acgaagacat cgtcagcttc cattgtcacc gagtacaggt
     1861 tgcggctgcc gcgggtggtc atcgcgaaca tccggcgcgg cgccagggcg gccaggcagc
     1921 tcggccagtc cgggtgacgg acgagcctgg catattcgtg gtagtcgagc ccggcccggc
     1981 gcaggtgctt gtcgctgaga tcgaagccga gcggctcgac gagatgcagt tcggcgccgg
     2041 tattggcgca catgcggatg atgttgccgg tattgggcgg aatttccgga tggacgagga
     2101 cgacggcgaa cacgggacgg tcggtgagcg gcaaaggcgc cgattaaatc acgaattcag
     2161 ctattgaggg tgcgggcgac gaccgcgaca tggacctcga gcgcgccgtg ttcgatcaag
     2221 ctgcgggcgg cttcatccgc ggtggcgccg ctggtcagca catcgtcgac cagaagaagg
     2281 cgctggccgc tgcaatccgc atggcaggcg aaggcgccgc gcacgttgcg ctgcctggcc
     2341 ttgcggtcga ggccggcttg tgacgcggtg gcgcgttggc gttgcaggag gtcgcgggcg
     2401 agcgggagtt gccgcagccc ggcaatcgtg cgcgcaattt cca
//
LOCUS       DAOSDX010000421         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15085_length_2439_cov_3.341023, whole genome shotgun sequence.
ACCESSION   DAOSDX010000421 DAOSDX010000000
VERSION     DAOSDX010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15085_length_2439_cov_3.341023"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLN02_09505"
     CDS             complement(<1..603)
                     /locus_tag="PLN02_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOI52601.1"
                     /translation="MSTSLIGRPLPHESARLHVSGKAHYVDDLPQAAGTLHAAPGLST
                     IARGRIRSMDLSAVRAYPGVRAVLTAADIPGDNNCGPILHDDPILADGEIRFHGQPLF
                     VVAAETRDIARQAVRLAKIDYEIEPPILDMDAAIAAESWVLPPFSMQRGAVDAAFADA
                     PHRLSGNAVLGGQEHFYLEGQISYAQPGDDGTLHVLCSTQH"
     gene            complement(600..2093)
                     /gene="xdhA"
                     /locus_tag="PLN02_09510"
     CDS             complement(600..2093)
                     /gene="xdhA"
                     /locus_tag="PLN02_09510"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HOI52602.1"
                     /translation="MSERPIQFYFRGAIQSVQGPAPTRTVLQYLREDLQQTGTKEGCA
                     EGDCGACTVVIGELVGEPGAEELRLRAVNACIQFLPSLDGKALFTVEDLAAGEQLHPV
                     QQALVDRHGSQCGFCTPGFAMSLFALYENQEHCPERDEIDHTLSGNLCRCTGYRPIVD
                     AARDAYALPRRRLDRLPVIAALKTLADLPPLDYLGDGRRSHSPRTLAELAELRERLPD
                     ARLVAGGTDVGLWVTKQGRDLGELILTGAVAELKRIQIGPGRLEIGAGVTLTDAFAAL
                     TAFEPQWAELARRFASTPVKNAGTLGGNVANGSPIGDSMPALIALGARIVLQRGAATR
                     EMALEDFYLDYQKTALQSGEFVRALVLDAPAAGLQRHFRSYKVAKRLDQDISAVACGL
                     AVHVDDEGCIRRARLAFGGMAATPKRAAGAEAALLGRPWDAAAVRRAMDALGSDFAPF
                     TDVRASSAYRLSVAANLLQRFWLETSGAADAPVRLAALAPIAPEDKA"
BASE COUNT          386 a          770 c          881 g          402 t
ORIGIN      
        1 gtgctgggtc gagcagagca cgtgcagcgt cccgtcgtcg cccggctggg cataggaaat
       61 ctgcccttcg aggtagaaat gttcctgtcc accgagcacg gcgttgccgc tcagacggtg
      121 cggggcgtcg gcaaaggcgg cgtcgacggc gccgcgctgc atggaaaacg gcggcaggac
      181 ccaggattcg gcggcaatcg ccgcgtccat gtcgaggatg ggcggctcga tctcgtagtc
      241 gatcttcgcc agacgcaccg cctggcgggc gatgtcgcgc gtctcggcgg cgacgacgaa
      301 gagcggctgg ccgtgaaaac ggatttcgcc atcggcgagg atggggtcgt cgtgcaagat
      361 cggcccgcag ttgttgtcgc cgggaatgtc ggcggcggtg agcacggcgc gtacgccggg
      421 ataggcgcgc acggcgctga gatccatgct gcggatgcgg ccacgggcga tggtcgataa
      481 accgggcgcc gcgtgcagcg tgccggcggc ttgcggcagg tcgtcgacgt agtgggcctt
      541 gccgctgacg tgcaggcggg cgctttcgtg cggtagcggg cggccgatca gcgaggtgct
      601 catgccttgt cctccggggc aatcggcgcc agcgcggcaa ggcggaccgg cgcgtcggcg
      661 gcgcccgagg tttccagcca gaagcgctgc agcaggttgg cggcaacgct caggcggtag
      721 gcgctgctcg cgcggacatc ggtgaagggg gcgaaatcgc tgcccagcgc gtccattgcc
      781 cggcgcacgg cggcggcgtc ccagggccgg ccgagcagcg ccgcttcggc gccggccgcc
      841 cgtttcgggg tcgccgccat gccgccgaag gcgaggcggg cgcggcggat gcagccttcg
      901 tcgtcaacat ggacggccag cccgcaggca accgccgaga tgtcctggtc cagccgcttg
      961 gccaccttgt agctgcggaa atgccgttgc agcccggccg ccggcgcatc aaggacgagc
     1021 gcgcggacga actcgccgct ctgcagcgcc gtcttctggt aatcgaggta gaaatcttcc
     1081 agcgccattt cgcgcgtcgc cgcgccgcgc tgcagcacga tgcgcgcgcc gagggcgatc
     1141 agcgccggca tcgaatcgcc gatcggcgaa ccgttggcga cgttgccgcc gagcgtgccg
     1201 gcgttcttga ccggggtcga ggcgaagcgc cgggccagtt cggcccattg cggctcgaag
     1261 gcggtgagcg ccgcaaaggc gtcggtcaac gttacgccgg cgccgatttc cagccggccg
     1321 gggccgatct ggatgcgctt gagttcggcg acggcgccgg tcaggatcag ttcgccgaga
     1381 tcgcgcccct gcttggtcac ccacaggccg acgtcggtgc caccggcaac cagccgggcg
     1441 tccggcagcc gctcgcgcag ctcggcgagt tcggccagcg tgcgcgggct gtggctgcgc
     1501 cggccgtcgc cgaggtaatc gagcggcggc aggtcggcca atgttttcaa ggcagcgatc
     1561 accggcaggc ggtcgaggcg gcggcgcggc agcgcgtagg cgtcgcgggc ggcatcgacg
     1621 atcggccggt agccggtgca gcggcacagg ttgccggaca gcgtgtggtc gatctcgtcg
     1681 cgttccgggc aatgttcctg gttttcatag agggcgaaca gcgacatcgc gaagcccggc
     1741 gtgcagaaac cgcactgcga gccgtgccgg tcgaccagcg cctgctgtac cgggtgcagt
     1801 tgctcgccgg ccgccaggtc ttctaccgtg aacagcgcct tgccgtcgag cgaaggcagg
     1861 aactggatgc aggcattgac cgcgcgcagg cgcaattcct cggcaccggg ttcgccgacc
     1921 agttcgccga tcaccaccgt gcacgcgccg caatcgcctt ccgcgcagcc ttccttggtg
     1981 ccggtctgct gcaggtcttc gcgcaggtac tgaagcaccg tgcgcgtcgg cgccggccct
     2041 tgcaccgact ggatggcacc gcggaaatag aactggatcg gacgttcgga catggctgca
     2101 agcttcgctg atggcatggg ccgaaattag cacagggcag acgcgccggg cagcgtctgt
     2161 catggataag gactatgtga cgtggcttat ggttctcccg ccagctaacg ggcaggcgat
     2221 cgcaggcgat ctcattcggc ggatagagtc aaaatggaaa accctgggaa ccttgtcatt
     2281 tcgccccatg cgcctttaag accttgatca ggtggggctg aggactattc gcggattgca
     2341 gtgcggtcaa tcctttggca ttttttacgt ttacgccgac acctttggca atcagcaaat
     2401 tcaccatgtc gagattgtcg tgaactggaa tgactctgg
//
LOCUS       DAOSDX010000422         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15092_length_2438_cov_2.619387, whole genome shotgun sequence.
ACCESSION   DAOSDX010000422 DAOSDX010000000
VERSION     DAOSDX010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15092_length_2438_cov_2.619387"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..997)
                     /locus_tag="PLN02_09515"
     CDS             complement(113..997)
                     /locus_tag="PLN02_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI52603.1"
                     /translation="MIPIRYVEPVFRPPSEAESLILPVTDGCSWNQCTFCEMYTAPQK
                     KFRARDEAEVLESIRATGERYGSDIRRVFLADGDALVLPTRRLLTVIEAIRTYLPAVR
                     RISSYCLPRNLKKKSQQEIDELAAAGLSMVYVGAESGDDAVLAAVSKGETFDSTVDAL
                     NKLGAAGISRSVMILNGLGGRELSRQHAENSARLANATQPEYLATLVVSFPKGEQRLR
                     AGFPGWEPLTQPELFAEMETFLSGLELKRTVFRSDHASNWLILKGTLGADKERLLAQV
                     RQAIAGEAPLRPAWARGL"
     gene            complement(994..1422)
                     /locus_tag="PLN02_09520"
     CDS             complement(994..1422)
                     /locus_tag="PLN02_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOI52604.1"
                     /translation="MPRIKIALPEKFVFSTEIPIYIQHINYGHHLDNAALLMLVAEAR
                     VRFFKHFGWSELDVGGPGIVVADVAAQYRSEAFHGETLVIAFAAADFHDYGCDLVWQL
                     NDQASGREVARGKHGIVFFDYATRSKVKVPTVFAEKMAGA"
     gene            complement(1495..>2438)
                     /locus_tag="PLN02_09525"
     CDS             complement(1495..>2438)
                     /locus_tag="PLN02_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOI52605.1"
                     /translation="PAKPGAAGGDAGAGGKAEKPAAKIDLSALEALNFRGKLAIGQLQ
                     AKGLKLSALAARLGAADGRLDVSSMQAKLYGGSLDGALGVNARGNVFTVRQNLSGIDI
                     RPLLADLIHKEPLEGRGTVTLNVNTRGATVDALKRGLAGQASLALKDGAVRGVNLAKQ
                     LRDAKAALRGGKSSASNADATEKTDFSELVASFRIAGGVAHNDDLAMKSPFLRLAGNG
                     DIDIGNSRIDYLAKVSVVETSKGQEGKELADLKGITVPIRLRGPFDNLSYSLEAGELL
                     KDVAKAILDEKKEELKEKAGKKLEEKLGDKLKGLFGK"
BASE COUNT          377 a          797 c          808 g          456 t
ORIGIN      
        1 atcttcgggc ggctgatgga cagcggcaat ttcagcggcg tgttgttcgg tatcgccatc
       61 ttccaggcgc tcgccatctt caccgcgctg cgcgtcggca aagcgggctg agctacaggc
      121 cgcgcgccca ggccgggcgc agcggcgctt cgccggcgat ggcctgacgc acctgggcaa
      181 gcaggcgttc cttgtcggcg ccgagcgtgc ccttgaggat cagccagttc gacgcgtggt
      241 cggaacggaa caccgtgcgc ttgagttcca ggccgctcag gaaagtctcc atctcggcga
      301 acaactccgg ctgggtcagc ggctcccagc cgggaaaacc ggcgcgcagg cgctgttcgc
      361 ccttgggaaa gctgaccacc agcgtcgcca ggtattccgg ctgggtggcg ttggccaggc
      421 gcgccgagtt ctccgcatgc tggcgcgaca gttcgcgccc accgagaccg ttgagaatca
      481 tcaccgaacg gctgatgccg gcggcgccga gcttgttcag cgcgtcgacg gtcgagtcga
      541 aggtttcgcc cttgctaacc gcggcgagca ccgcgtcgtc gccggattcg gcgccgacat
      601 agaccataga gaggccggca gcggcgagtt cgtcgatttc ctgctggctt ttcttcttca
      661 gattgcgcgg caggcagtag ctggaaatcc gccgcaccgc tggtagatag gtgcggatcg
      721 cctcgatcac ggtcaaaagc cgccgcgtcg gcaacaccag cgcatcgccg tcggccagga
      781 agacgcggcg gatatcgctg ccgtaacgtt cgccggtggc gcggatgctc tccagcacct
      841 cggcctcgtc acgggcgcgg aatttcttct gcggcgcggt gtacatctcg cagaaagtgc
      901 actggttcca cgaacagccg tcggtcaccg gcaggatcag cgactcggct tccgacggcg
      961 gccggaacac cggctcgacg tagcgaatcg ggatcatgcg ccggccatct tctcggcgaa
     1021 taccgtcggc accttgacct tgctgcgcgt cgcgtagtcg aagaagacga tgccgtgctt
     1081 gccgcgcgcc acttcgcggc cgctcgcctg atcgttcagt tgccagacca ggtcgcagcc
     1141 gtaatcgtgg aaatcggcgg cggcgaaggc gatcaccagc gtctcgccgt ggaaggcttc
     1201 ggaacggtac tgggcggcga catcggcaac gacgatgccg ggcccgccga cgtcgagctc
     1261 gctccagccg aaatgcttga agaagcgcac ccgtgcctcg gcgaccagca tcagcagcgc
     1321 ggcgttgtcg aggtggtggc cgtagttgat gtgctggatg tagatcggaa tttcggtgct
     1381 gaaaacgaac ttctcgggca gggcgatctt gatgcggggc atggcgtcaa tccggcggat
     1441 aaaggcggca ttctaggccg gcagcgcgcg cggcgcacgc tcccggccgg tggctcattt
     1501 gccgaacagg cccttgagtt tgtcgccgag tttttcttcc agcttcttgc cggccttttc
     1561 cttcaactcc tccttcttct cgtcgagtat ggctttcgcc acatccttga gcaattcgcc
     1621 ggcttccagc gaataggaaa gattgtcgaa ggggccgcgc aggcggatcg gcaccgtgat
     1681 gcccttcaag tcggcgagtt ccttgccttc ctgccccttg gaggtttcaa cgacgctgac
     1741 tttcgccagg tagtcgatac ggctgttgcc gatgtcgatg tcaccgttgc cggccaagcg
     1801 caggaagggc gatttcatcg ccaggtcgtc gttgtgtgcg acgccgccgg caatccggaa
     1861 gctggcgacc agttccgaga agtcggtctt ttccgtggcg tcggcgttgc tcgcgctgct
     1921 cttgccgccg cgcagggcag ccttggcgtc gcgcaactgt ttggccagat tgacgccgcg
     1981 cacggcgccg tccttcagtg ccagcgaggc ctgcccggcc agcccgcgct tcagcgcatc
     2041 gaccgtggcg ccacgggtat tgacgttgag cgtcaccgtg ccgcgacctt ccagcggctc
     2101 cttgtggatc aggtcggcga gcagcgggcg gatgtcgatg ccggagaggt tctggcggac
     2161 cgtaaacaca ttgccccggg cattgacgcc gagcgcgccg tcgaggctgc cgccgtagag
     2221 cttcgcctgc atcgacgaga cgtccaggcg gccgtcggcg gcgcccaggc gcgccgccag
     2281 tgccgagagt ttcaggccct tcgcctgcaa ctggccgatg gccagcttgc cgcggaagtt
     2341 gagcgcttcg agcgcgctca ggtcgatctt cgccgccggc ttctccgcct tgccgcctgc
     2401 gccagcatcg ccgcccgccg cgcccggctt ggccggcg
//
LOCUS       DAOSDX010000423         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15206_length_2423_cov_2.895693, whole genome shotgun sequence.
ACCESSION   DAOSDX010000423 DAOSDX010000000
VERSION     DAOSDX010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15206_length_2423_cov_2.895693"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PLN02_09530"
     CDS             <1..1084
                     /locus_tag="PLN02_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOI52606.1"
                     /translation="EKRDLERELQARQRDLERVRPAVAAVRQLDLRLNARRDGRVRLR
                     YLFAEAGWRPAYRAKLDSDGRRVEIERLAQIAQRSGEDWSKVELALSTGQPQQRVAGP
                     LPDPWNLSLRPEVRPEARAVMPMMAAAPAPMAKRGVAADQAAEPLFEVAVTQSEFATE
                     YAIPGRIDLPADGRKVAVALGSLQVPVELHAQVAPRQDKAAYLVARGALPEGVWPAGP
                     LQIYRNGAYVGQTTWDPAQETRLELPFGRDEKIKVAVRNLAADQARAGFVGQQAERRL
                     ADVFTVTNQHARPVDIRVIDASPVARDSQIEVEASFQPPVTANDWDDRPGVVAWEQRL
                     DAGAAASFTADYRIRWPKERKVIGLP"
     gene            1110..1976
                     /locus_tag="PLN02_09535"
     CDS             1110..1976
                     /locus_tag="PLN02_09535"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI52607.1"
                     /translation="MKIGFIGLGIMGRPMALNLLKGGHQVSVWARRAESMQPLLDAGA
                     SGAASLADVARGQELVISMVADAPDVAEVMRGVASAGTSGLVAVDMSTIAPAAAKAIH
                     AELAAAGVDFVDAPVSGGEVGAIAGTLAIMAGGSDAAFARAEPAFACLGKNVVHVGGP
                     GAGQVTKAANQIVTGMGVLAVAEAMAFASKNGVDRAKVREALLGGFAYSKILENHGQR
                     MLDRNFKPGFKSWMHEKDLNIVMQTAHELGLCLRGSAATAQMFNAMVGSGLGEEDSIA
                     VLKLLENLSGQA"
     gene            1973..>2423
                     /locus_tag="PLN02_09540"
     CDS             1973..>2423
                     /locus_tag="PLN02_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOI52608.1"
                     /translation="MKVGFIGLGAMGRPMALHLQAAGHELHVWARRPASADGLPATVH
                     PTPAVLGAACEVVFTVITSSADVEGVALGDNGLIHGMAPGSVLVDCSTIAPDVARRIA
                     GQLAEKGIHMLDAPVSGGEQGAIAATLAIMAGGDAAVLERVRPLLEKL"
BASE COUNT          379 a          839 c          863 g          342 t
ORIGIN      
        1 ggagaagcgc gatcttgaac gcgaactgca ggcccgccag cgcgacctcg aacgcgtccg
       61 cccggcggtc gccgccgtcc gccagctcga cctgcgcctg aacgcgcggc gtgatggccg
      121 cgtccgcctg cgctacctgt tcgccgaggc cggctggcgg ccggcttacc gcgccaagct
      181 cgacagcgac ggccggcggg tcgagatcga gcgtctggca cagatcgccc agcgcagcgg
      241 cgaggactgg agcaaggtcg aactcgcgct atccaccggc cagccgcagc agcgcgtcgc
      301 cggtccgctg cccgatccct ggaacctctc gctgcgtcca gaagtgcgcc ccgaagcgcg
      361 cgccgtgatg ccgatgatgg cggccgcccc ggcgcccatg gcgaaacgtg gcgtggcggc
      421 ggaccaggct gccgagcctt tgttcgaggt agcggtgacg cagagcgaat tcgccaccga
      481 atacgcgatt cccggccgca tcgacctgcc ggccgacggg cgcaaggtgg cggtggcgct
      541 gggcagcctg caggtgccgg tggagctgca cgcgcaggtg gcgccgcgcc aggacaaggc
      601 cgcctacctg gttgcccgcg gcgcgctgcc ggaaggcgtc tggcctgccg gtccgctgca
      661 gatctaccgc aacggcgcct acgtcggcca gaccacctgg gatccggcgc aggaaacgcg
      721 cttggagctg cccttcgggc gtgacgagaa gatcaaggtg gcggtgcgca acctggccgc
      781 cgaccaggcg cgcgccggtt tcgtcggcca gcaggcggaa cggcgtctcg ccgacgtgtt
      841 cacggtgacc aaccagcacg cgcggccggt cgacatccgc gtcatcgatg ccagcccggt
      901 tgcccgcgac agccagatcg aggtcgaggc cagcttccag ccgccggtca ccgctaatga
      961 ctgggacgac cggccaggtg tcgtcgcctg ggaacagcgt ctcgatgccg gcgcggcggc
     1021 cagcttcacc gccgattacc gcatccgctg gccgaaagaa cgcaaagtca tcggcctgcc
     1081 ctgaacccca aacgaacgga gataacaaaa tgaagatcgg attcatcggc cttggcatca
     1141 tgggccgccc gatggccctc aacctgctca agggcggcca ccaggtcagc gtctgggcgc
     1201 gccgtgccga atcgatgcag cccttgctcg acgctggcgc cagcggcgcc gcgtcgctcg
     1261 ccgacgtcgc gcgcggccag gaactggtga tctcgatggt cgccgacgcg ccggatgtcg
     1321 ccgaagtcat gcgcggcgtc gccagcgccg gcaccagcgg cctggtcgcc gtcgacatga
     1381 gcaccatcgc cccggcggca gccaaggcca ttcacgccga actggcggcg gccggcgtcg
     1441 atttcgtcga tgcgccggtg tccggcgggg aagtcggggc gattgccggc acgctggcta
     1501 tcatggccgg cggttccgac gccgctttcg cccgcgccga gccggcgttc gcctgcctgg
     1561 gcaagaacgt cgtccacgtc ggcggtcccg gtgccgggca ggtgaccaag gcggccaacc
     1621 agatcgtcac cggcatgggc gtgctggcgg tcgccgaggc gatggccttc gcgtcgaaaa
     1681 acggcgtcga tcgcgccaag gtgcgcgagg cgctgctcgg cggcttcgcc tactcgaaga
     1741 tcctggaaaa ccacggccag cgcatgctcg accgcaactt caagccgggt ttcaagagct
     1801 ggatgcatga gaaggacctg aacatcgtca tgcagacggc gcacgaactc ggtctgtgcc
     1861 tgcgtggttc ggcggcaacc gcgcagatgt tcaacgcgat ggtcggctcg gggctgggcg
     1921 aggaggattc gatcgccgtg ctgaagcttc tggaaaacct gtcggggcag gcatgaaggt
     1981 cggtttcatc gggctgggcg cgatgggtcg cccgatggcc ctgcacctgc aggcggccgg
     2041 ccacgaactg cacgtctggg cgcgccgccc ggccagcgcc gacggcctgc cggccacagt
     2101 gcacccgacg ccggccgtgc tcggcgcggc gtgcgaggtg gtgttcacgg tcatcacttc
     2161 cagcgccgac gtcgaaggtg tggcgcttgg cgacaacggc ctgatccacg gcatggcgcc
     2221 gggcagcgtg ctggtcgatt gctcgaccat cgctcccgac gttgcccggc gcatcgccgg
     2281 ccaactggcg gagaagggca tccacatgct cgacgcgccg gtctccggcg gtgagcaggg
     2341 ggcgattgcc gcgacgctgg cgatcatggc cggcggcgac gcggcggtgc tcgaacgggt
     2401 gcgcccgctg ctcgaaaaac tcg
//
LOCUS       DAOSDX010000424         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15223_length_2421_cov_3.191462, whole genome shotgun sequence.
ACCESSION   DAOSDX010000424 DAOSDX010000000
VERSION     DAOSDX010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15223_length_2421_cov_3.191462"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1051)
                     /gene="ylqF"
                     /locus_tag="PLN02_09545"
     CDS             complement(110..1051)
                     /gene="ylqF"
                     /locus_tag="PLN02_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOI52609.1"
                     /translation="MSIQWYPGHMTQARKKAGETLAMADVVVEVLDARLPQASSNPMI
                     HELRTFRQRPCLKLLNKADIADPAVTKAWLDYFGKQPGVMAVAISCKKPGDVAKIPDL
                     AQKLAPHRNDAVKPLRLMIMGIPNVGKSTLMNALVKKKVAAVGDQPAVTKSQQRIDLS
                     SRLTIYDTPGMLWPKIDHPIDGLMMAASHSVGVNAYIDDEVATWLAGYLLEHYPALLQ
                     ARYGFELPGMDAVAVLEGIAKKRGCIIKGRGGELDLEKAAGILLNDYRTGTLGRISLE
                     TPTLRASRQAELEKAAKSTAVAEASPETASTDEDAEH"
     gene            complement(1082..>1330)
                     /locus_tag="PLN02_09550"
     CDS             complement(1082..>1330)
                     /locus_tag="PLN02_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOI52610.1"
                     /translation="VESSVESSVESSVESSVESSVETADKILGLLAEHSTLTLAELAK
                     CLNLSVRAVEKQVARLKAQGRLRRIGPNKGGRWEVSGA"
     assembly_gap    1331..1430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1431..2387)
                     /locus_tag="PLN02_09555"
     CDS             complement(<1431..2387)
                     /locus_tag="PLN02_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52611.1"
                     /translation="MAIRVPRASRQQRPIHLTTNPFGNTWRRLNDGDRALADEDIKRM
                     LAEQVEDSRDTRILPNFGLSDLNADSLKAYRNIFRADKPDHPWLALDDQAFLQRLGGW
                     REDRTSGETGLTQAGLLMFGQWPAIAEALPLYFVDYQERPRDYAESVQWLDRLVPDGS
                     WSGNLFDFYRRVVIKLTAELKVPFVLQGDLRQDDTPTHKALREALVNALVHADYSGRV
                     SVQVVKEPAGFTFRNPGTLRISPSQALQGGISDCRNRTLQQMFLMIGLGERAGSGMSR
                     IVHGWQALGHGLQLREYFDPQEYTVLEMRWSDELLQDGSPKSS"
BASE COUNT          401 a          708 c          726 g          486 t
ORIGIN      
        1 accagggacc tacggattaa cagtccggcg ctctaccgac tgagctatcg aggaaaagtc
       61 ccgccgtctg gcgagaggcg cggattataa ccagcttttc cgaaaaggcc tagtgctcgg
      121 cgtcttcgtc ggtcgatgcg gtttccggcg aggcttcggc gaccgccgtc gacttggcgg
      181 ccttttccag ttcggcctgg cggctggcgc gcagggtcgg ggtttccagg ctgatgcggc
      241 cgagggtgcc ggtgcggtag tcgttgagca ggatgccggc ggccttttcg aggtcgagtt
      301 cgccgccccg gcccttgatg atgcagccgc gctttttggc gatgccttcg agcacggcaa
      361 ccgcgtccat gcccggcaac tcgaagccgt agcgggcctg gagcagcgcc gggtagtgtt
      421 cgagcaggta gccggcgagc caggtggcga cttcgtcgtc gatgtaggcg ttgacgccga
      481 ccgaatggct ggccgccatc atcaggccgt cgatcgggtg gtcgatcttc ggccacagca
      541 tgcccggcgt gtcgtagatg gtcagccgcg aactgaggtc gatgcgctgc tggctcttgg
      601 tcaccgccgg ctggtcgccg acggcggcga ccttcttctt gaccagcgcg ttcatcagcg
      661 tcgatttgcc gacgttgggg atacccatga tcatcagccg cagcggcttg accgcgtcgt
      721 tgcgatgcgg cgccagcttc tgtgccagat cggggatctt ggcgacgtcg ccgggtttct
      781 tgcaggagat ggcgaccgcc atcacgcccg gttgcttgcc gaagtaatcg agccaggcct
      841 tggtcactgc cgggtcggcg atgtcggcct tgttcagcag tttcaggcag gggcgctggc
      901 ggaaggtgcg cagctcgtgg atcatcgggt tgctggaagc ctgcggcagg cgggcgtcga
      961 gcacttcgac gacgacatcg gccatggcca gcgtctcgcc cgctttcttg cgggcctggg
     1021 tcatgtgacc gggataccat tggatggaca tgtttgctct ctgttctatc ttgactgttt
     1081 ttcaggcacc ggagacttcc cagcggccac ccttgttcgg gccgatgcgg cgcaaacgtc
     1141 cttgcgcctt gagtcgggca acctgttttt cgactgcgcg cacgctgagg ttaaggcact
     1201 ttgccagttc cgcaagcgtc agcgtcgaat gttccgccaa aagacccaga atcttgtcgg
     1261 cagtttctac cgaactttct accgaacttt ctaccgaact ttctaccgaa ctttctaccg
     1321 aactttctac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgaacttttc
     1441 ggtgaaccgt cctgcagaag ttcatcagac caacgcattt ccaatacggt gtattcctgc
     1501 ggatcgaaat actcacgcaa ttgcagaccg tggcccagcg cttgccagcc gtgaacgata
     1561 cgcgacatgc ccgacccggc gcgttcgccc aagccgatca tcaggaacat ctgctgcagg
     1621 gtgcgattgc ggcaatcact gataccaccc tgcaaagcct gactgggcga aatacgcaag
     1681 gtgcccggat tgcggaaggt gaaaccggcc ggctccttga ccacctggac ggaaacgcgg
     1741 ccgctgtaat cggcatgcac caacgcattg accagtgcct cgcgcagcgc cttgtgcgtg
     1801 ggtgtatcgt cctgtcgcag gtcgccctgc aggacgaagg gcactttcaa ctcggcagtc
     1861 agtttgatga ccactcgtcg ataaaagtcg aacaggttac ccgaccagga accgtccggc
     1921 accagccgat ccagccattg cacgctttcg gcgtagtcac gcgggcgttc ctggtaatcg
     1981 acaaagtaca acggcagcgc ttcggcaatc gccggccact gcccgaacat cagcagcccg
     2041 gcctgagtca gcccggtttc tccgctggta cggtcttcgc gccagccgcc gagcctttgc
     2101 aggaaagcct gatcgtcgag cgccagccag ggatgatccg gtttgtcggc acggaaaatg
     2161 ttgcggtagg ccttgaggct gtccgcattc aggtcgctta ggccaaaatt cggcaggatg
     2221 cgcgtatcgc ggctgtcttc gacctgctca gcgagcatcc gcttgatgtc ttcatcggcc
     2281 agcgcgcggt cgccgtcgtt caggcgacgc caggtattgc cgaagggatt ggtcgtcaga
     2341 tgaatcggcc gctgctgccg gctggcccgt ggaacgcgga tcgccaacgg ggtcttgccg
     2401 gcgatgtcga tttcctcgac a
//
LOCUS       DAOSDX010000425         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15273_length_2415_cov_3.454237, whole genome shotgun sequence.
ACCESSION   DAOSDX010000425 DAOSDX010000000
VERSION     DAOSDX010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15273_length_2415_cov_3.454237"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..845
                     /locus_tag="PLN02_09560"
     CDS             117..845
                     /locus_tag="PLN02_09560"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOI52612.1"
                     /translation="MTPGELLPPEVASAPEPPAPAVTPPAAPVLAGSGRLAFVVVKDT
                     LGMQIGRAEHRWEFPGDGTYRLQAMIETSGLAALFKPLRQEHESRGRLGPGGLQPESF
                     RVLRNGVSRGEDADFDWATRQVRLQRDGSVQSVASGSQDLLSLNYQLAYLAAPENGST
                     IGVVTGRKYMSYDLDAFGEETIETPAGSFRTLHLRAAGDTITEIWIALDRWRLPVKIR
                     FTDKKGDSYELRATEIDIAEAEPQ"
     gene            842..2110
                     /locus_tag="PLN02_09565"
     CDS             842..2110
                     /locus_tag="PLN02_09565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOI52613.1"
                     /translation="MTTKARFTPALFAHAEHLLGQLLRFDHPADAVVSHYFREHRQLG
                     HADRAFVAETVFAFLRRRRSLDARCGERPDNRQRLLATLAVARGWSQRELAPVLKASE
                     EAWLAGVKAVRDDDLPPAVRCDLPDWLYERLLAQYSDEEIRALAAALNRPAPLDLRVN
                     TLKIDRERLLARLAGDGIGAAPGRWSPLAVRLRDKPALAKNPAFLDGSFEVQDEGSQL
                     LGLLLEARRGEMVVDFCAGAGGKTLLLGAQMKNTGRLYAFDVSDKRLANLKPRLARSG
                     LSNVHPVRIEHERDLKVKRLAGKADRVLVDAPCSGLGTLRRNPDLKWRQDAGAVAELT
                     AKQAAILASAATLVRPGGRLVYATCSLLREENAAVVEAFLAAHPGFVLVPADGVLARF
                     GCELPGAMLQLLPQQHDTDGFFAAVLERQA"
     gene            2107..>2415
                     /locus_tag="PLN02_09570"
     CDS             2107..>2415
                     /locus_tag="PLN02_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HOI52614.1"
                     /translation="MNDSGVAFGRLISHLLDDFHEPSFVWQVVALLCALGLAWSIARW
                     WRAAHPPEAGWHLRQVGSRLAFPLSGMLLTGAAMLILKHFFPVTLLKLAMSLLASMAL
                     V"
BASE COUNT          350 a          880 c          813 g          372 t
ORIGIN      
        1 cacccgcgca gccggcaccg ccgccgcccg cggcgaagaa acccaagctg ccgaccccgg
       61 cgccggccag cgcgccgccg ccgcttgtgc cgcccagtag cgcgccggaa gacgccttga
      121 cgccgggcga actgctgccg cccgaggtgg catcggcgcc ggaaccgccg gctcctgccg
      181 taacaccgcc ggcggcgccg gtgctggccg gcagcggccg gctcgccttc gtcgttgtca
      241 aggatacgct cggcatgcag atcggccgcg ccgaacaccg ctgggagttt cccggcgacg
      301 gcacttaccg cctgcaggcg atgatcgaga ccagcggtct ggccgcgctg ttcaagccgc
      361 tacgccagga gcacgagagc cgtggccggc tggggccggg cggcctgcag ccggaaagtt
      421 tccgcgtcct gcgcaacggc gtgtcgcgcg gcgaggacgc cgatttcgac tgggcgacgc
      481 gccaggtccg gctgcagcgc gatggcagcg tgcagtcggt ggccagcggc agccaggacc
      541 tgttgtcgct caactatcag ctggcctacc tggcggcgcc ggaaaacggc agcacgatcg
      601 gcgtcgtcac cggccgcaag tacatgtcct acgacctcga tgccttcggg gaagaaacca
      661 tagaaacgcc ggccggcagt ttccgcacct tgcacctgcg ggccgccggc gacaccatca
      721 ccgaaatctg gatcgccctc gaccgctggc gactcccggt taaaatccgc ttcaccgaca
      781 agaagggcga cagttacgaa ttgcgcgcca ccgagataga tattgccgag gccgaaccgc
      841 agtgaccacg aaagcccgtt tcacacccgc cctgtttgct cacgccgaac acctgctcgg
      901 ccagttgttg cgcttcgacc atccggccga cgccgtcgtc tcgcattact tccgcgagca
      961 ccgccaactc ggtcacgccg accgcgcctt cgtcgccgag acggtgttcg ccttcctgcg
     1021 ccgccggcgc agcctggacg cgcgctgcgg cgaacgtccg gacaaccggc agcgcctgct
     1081 ggcgacgctg gccgtcgccc gcggctggag ccagcgcgag ctggcgccgg tgctgaaggc
     1141 gagtgaggag gcctggctgg ccggcgtcaa ggcggtgcgc gacgacgacc tgccgccggc
     1201 ggtgcgctgc gacttgccgg actggttgta cgagcgcctg ctggcccagt acagcgacga
     1261 ggaaatccgc gccctggccg ccgccctcaa ccggccggcg ccgctcgacc tgcgcgtcaa
     1321 caccctgaag atcgaccgcg agcgcttgct cgcccggctc gccggcgacg gcatcggcgc
     1381 cgcgcccggg cgctggtcgc cgctcgccgt gcgcctgcgc gacaagccgg cgctggcgaa
     1441 aaacccggcc ttcctcgatg gcagtttcga ggtccaggac gagggcagcc agttgctcgg
     1501 cctgctgctc gaagcgcggc gcggcgagat ggtcgtcgat ttctgtgccg gtgccggcgg
     1561 caagacgctg ctgctcggcg cacagatgaa gaacaccggt cgtctctacg ccttcgacgt
     1621 ttccgacaag cggctcgcca acctgaagcc gcgcctggcg cgttccgggc tgtccaacgt
     1681 ccatccggtg cgcatagagc acgagcgcga cctcaaggtc aagcgcctgg ccggcaaggc
     1741 cgaccgcgtg ctggtcgatg cgccctgttc cgggctcggc acgctacgcc gcaatcccga
     1801 cctgaaatgg cgtcaggacg ccggcgcggt cgccgaactg accgccaagc aggcggccat
     1861 cctggcctcg gcggcgaccc tggtgcgccc cggcgggcgc ctggtgtacg cgacctgcag
     1921 cctgctgcgc gaggaaaatg cggcggtggt cgaggccttc ctcgccgctc atcccggctt
     1981 cgtcctggtg ccggcggacg gcgtgctggc ccgcttcggc tgcgagttgc ccggtgccat
     2041 gctgcaactg ctgccgcagc agcacgatac cgacggcttc ttcgccgccg tcctggagcg
     2101 ccaggcatga acgattccgg cgtcgctttc ggcaggctga tcagccacct gctcgacgat
     2161 ttccacgagc ccagcttcgt ctggcaggtg gtggccttgt tgtgcgcgct tggcctggcc
     2221 tggagcattg ctcgctggtg gcgggcggcg catccgccgg aagccggctg gcacctgcgc
     2281 caggtcggca gccggctggc cttcccgctt tccggcatgt tgctgaccgg cgccgcaatg
     2341 ctgatcctca agcacttctt tccggtcacc ctgctcaagc tggcgatgtc gctgcttgct
     2401 tcgatggcgc tcgtg
//
LOCUS       DAOSDX010000426         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15276_length_2415_cov_3.031356, whole genome shotgun sequence.
ACCESSION   DAOSDX010000426 DAOSDX010000000
VERSION     DAOSDX010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15276_length_2415_cov_3.031356"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..704)
                     /locus_tag="PLN02_09575"
     CDS             complement(21..704)
                     /locus_tag="PLN02_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HOI52615.1"
                     /translation="MLIPIEGVLSKDEVRQFRELLDVAPWEDGLKTAGTLARAVKRNQ
                     QLADGSPLAVELGNRILRRLGNNPLFISAALPARIYPPKFNRYADGGTYGAHVDSAVM
                     QVPGTSISVRSDLSATLFLAEPDEYDGGELEIEGPFGIQAAKLAAGDIVLYPSSSLHR
                     VTPVTRGARIASFMWIQSMVQNDSSRTLLFDLDQAIQGMTGVVAPDDERLLKLTAVYH
                     NLLRQWAKP"
     gene            complement(698..1819)
                     /locus_tag="PLN02_09580"
     CDS             complement(698..1819)
                     /locus_tag="PLN02_09580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOI52616.1"
                     /translation="MQENRPPLTAIPAEIRCAQDYEAIAGHFLAADRLAYIAGGCGQE
                     TTLAANRAVFADLRIWPRLLRDVGQGHTALELFGRRLAHPILLAPLAYQTLAHPRGEI
                     DVARGAAASDSGFVLSTLAGRTLEEVAAQAGEERWFQLYFQAERRHTLDLVRRAEAAA
                     YRVLVVTLDTAIKLPSLRAQRAGFALPAAVVAANLADYPEVAPPVIERGQSRVFQGVM
                     AQAPGWADLDWLLGETRLPVVVKGVLHPEDARQLKARGVAGLIVSNHGGRSLDGAPAS
                     LAALPAIRAAVGDAYPLLLDSGIRSGGDVFKALALGADAVLVGRLQTYALAVAGALGV
                     AHLLRLLREELEVCMAQVGCATLADIGAAMIFKEKETRC"
     gene            complement(1872..>2415)
                     /locus_tag="PLN02_09585"
     CDS             complement(1872..>2415)
                     /locus_tag="PLN02_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI52617.1"
                     /translation="YATVGTFNTGENRVRGIEFGLAGNLTDKLSAFAGVTFMDSEVTK
                     SANPENKGKTLSNFADKTASAQLRYQATPKFAFGTAVKYESKKYAGQPDTAAGYVTAA
                     QVAAGSQPASNLGAYTQPIPAYTVWDLFAEYRVTKAAKLRLNVGNVFDKDYYLAGYRS
                     GSFLYKGDARNARLTLNYEF"
BASE COUNT          398 a          826 c          789 g          402 t
ORIGIN      
        1 gcatgcgttt ctccttgaaa tcagggcttg gcccactgcc gcaacaggtt gtgatagacg
       61 gcggtcagct tgagcagacg ctcgtcatcg ggcgcgacga cgccagtcat gccctggatc
      121 gcctggtcga gatcgaagag cagcgtgcgc gaactgtcgt tctgtaccat gctctggatc
      181 cacatgaagg aggcgatgcg cgcgccgcgc gtcaccggcg tcacgcggtg caggctgctc
      241 gacggataga ggacgatgtc gccggccgcc agcttcgccg cctggatgcc gaacggcccc
      301 tcgatttcca gttcgccgcc gtcgtactcg tcgggttcgg cgaggaacaa ggtcgccgag
      361 aggtcgctgc gtaccgaaat cgacgtaccc ggcacctgca tcaccgcgct atcgacatgc
      421 gcgccgtaag ttccgccatc ggcgtagcgg ttgaacttcg gaggatagat gcgcgccggc
      481 aaggccgccg agatgaacag cggattgttg ccgagtcgcc tgaggatgcg gttgcccaac
      541 tcgaccgcca gcggcgagcc gtcggccagt tgctggttgc gcttgacggc gcgggccagg
      601 gtgccggccg tcttcaggcc gtcttcccac ggcgcgacat cgagcagctc gcggaattgc
      661 ctgacttcat ccttgctcag gacaccctcg atcgggatca acatctcgtt tccttttcct
      721 tgaaaatcat cgccgcaccg atatcggcca gcgtcgcgca gcccacctgc gccatgcaaa
      781 cttccagttc ctcgcgcagc aggcgcagca gatgcgccac cccgagcgcc ccggcaaccg
      841 ccagcgcata ggtctgcaga cggccgacca gcaccgcgtc ggcgcccaag gccagcgcct
      901 tgaagacatc gccgccgctg cggatgccgc tgtcgagcag cagcggataa gcgtcgccga
      961 cggcggcgcg gatcgccggc agcgcggcga ggctggccgg cgcgccgtcg agcgagcgac
     1021 cgccatgatt ggagacgatc agcccggcaa cgccgcgcgc cttcagttgg cgagcgtcct
     1081 ccggatgcag gacgcccttg accaccaccg gcagacgcgt ctcgccaagc agccagtcga
     1141 ggtccgccca gcccggcgcc tgcgccatca ccccctggaa cacccggctc tgtccgcgct
     1201 cgatgacggg cggcgccact tcggggtaat ccgccagatt ggcggcgacc accgccgccg
     1261 gcagcgcaaa accggcgcgc tgggcgcgca ggctgggcag cttgatcgcc gtgtccagcg
     1321 tcaccaccag cacccggtag gcggcggcct cggcacgccg gacgaggtcg agcgtatgcc
     1381 ggcgctcggc ctgaaaatac agctggaacc agcgctcttc gccggcctgc gccgccacct
     1441 cttccagtgt ccgcccggcc agcgtgctca acacgaagcc gctatcgctc gccgcggcgc
     1501 cgcgcgcgac gtcgatttca ccccgcggat gagccagcgt ctggtaggcg agcggtgcca
     1561 gcagaatcgg atgcgccagg cggcgaccga acagctccag cgccgtatgc ccctggccga
     1621 catcgcgcag caggcgcggc cagatccgga ggtcagcgaa aacggcgcga ttcgccgcca
     1681 gcgtcgtctc ctgcccgcag ccgccggcga tgtaggcgag gcgatcggcg gccaggaaat
     1741 gcccggcgat cgcttcgtaa tcctgtgcgc aacgaatctc ggcgggaatg gcggtcagtg
     1801 gcggtcggtt ttcttgcatt gcatgccaac gggcgaaccc ggaggattcg cccgtttcct
     1861 tttgcccagg tttagaactc gtaattgagc gtcagcctgg cgttgcgggc atcgcccttg
     1921 tagaggaagg agcccgaacg gtagccggca aggtagtaat ccttgtcgaa gacgttgccg
     1981 acgttcaggc gcagcttggc cgccttggtc acccggtatt ctgcgaacag gtcccacacc
     2041 gtgtaggccg gaatcggctg ggtgtaggcg ccgaggttcg acgccggctg gctgccggcg
     2101 gcgacctgcg ccgccgtgac gtagccggcc gcagtgtcgg gctgccccgc gtacttcttg
     2161 ctctcgtact tgaccgccgt cccgaaggcg aatttcggcg tcgcctggta acgcaactgc
     2221 gccgacgccg tcttgtcggc gaagttgctc aaggtcttgc ccttgttctc cggatttgcc
     2281 gacttggtca cttccgagtc catgaaggtg acgccggcaa aggcgctcag cttgtcggtc
     2341 aggttgccgg ccagaccgaa ctcgatgcca cgcacgcggt tctcgccagt gttgaaggta
     2401 ccgaccgtcg cgtag
//
LOCUS       DAOSDX010000427         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15280_length_2414_cov_3.996609, whole genome shotgun sequence.
ACCESSION   DAOSDX010000427 DAOSDX010000000
VERSION     DAOSDX010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15280_length_2414_cov_3.996609"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..223)
                     /locus_tag="PLN02_09590"
     CDS             complement(17..223)
                     /locus_tag="PLN02_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52618.1"
                     /translation="MAATQRKASRTRVQPVVQRRVEYIAAVRYLDGSRELFYVRNADN
                     LADARELVMLEVDDVSSLMIAERH"
     gene            394..2064
                     /locus_tag="PLN02_09595"
     CDS             394..2064
                     /locus_tag="PLN02_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protein"
                     /protein_id="HOI52619.1"
                     /translation="MLFLTHSFNSLTQRLLSELAARGHAISIELDIADSVTEEAVARF
                     RPDLVLAPFLKRAIPESVWSVVPCLIVHPGIPGDRGPAALDWAIQRGETRWGVTVLQA
                     EAEMDAGPVWAAAEFPMRPASKGSLYRNEVTEAAVRAVLKAVERFAAGDFQPQRVPGA
                     AHPAIRQADRQIDWTTQPTADVLARLRAADGFPGVADELFGQPCQLFDAWPEGRLRGA
                     PGTLLARRETALCRATADGAVWIGHVKKPGGIKLPATVAFPEAAELPEAPLAGWWQAD
                     HPTWQDIAWREADGVGFLSFRFYNGAMGTAQCRRLTEAFGWATTRPVRVIVLDGGSDF
                     WSNGIHLNLIEAADSPADESWANINAIDDLAEAILRCQTQLTVAAVGGNAGAGGCFLA
                     RACDRVWVRDGVVLNPHYKNMGNLYGSEFWTYLLPPRVGADGARAIMQHRLPMPAAEA
                     VACGFYDACLPAPGFAVDVARRALEMAQAANFPAQLADKVARRAADEAAKPLAQYREE
                     ELAAMRRNFYGFDPAYHVARYHFVHKSPHSWTPRHLARHRDLDWIIPT"
     gene            2061..>2414
                     /locus_tag="PLN02_09600"
     CDS             2061..>2414
                     /locus_tag="PLN02_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52620.1"
                     /translation="MNAPVLRRLPAVLVIDDELRSQEALRRTLEEDFEVFCASSAAEG
                     WEILEMEEASAGIRVVLCDQRMPGTTGVQFLKEVRQRWPDIVRIILSGYTDAEDIISG
                     VNEAGIWQYLLKPWQP"
BASE COUNT          408 a          823 c          780 g          403 t
ORIGIN      
        1 tgctgaggcg aggaattcag tgtcgttcgg cgatcatcag gctgctgaca tcatccactt
       61 cgagcatcac cagctcgcgg gcatcggcga gattgtcggc attgcgcacg tagaacagtt
      121 cgcggctacc atcgagatag cggacggcgg cgatgtattc gacccgacgc tgaacgacgg
      181 gttgaacccg ggtacgggat gccttgcgtt gcgttgcagc catgatgttg ttctccctgt
      241 gacatttttg cgcatcgtag cgaatggcaa gtccctgaaa aatagcgcga aggtcataaa
      301 cgccctgttt tctaacgcga acaccgtgga aaacgcggcc acaaaacctt ccatgatgaa
      361 gacatgatga agaaaaatcc ccgcagcatg cgcatgttat tcctaacgca tagcttcaac
      421 agcctgaccc aacgcctgct cagcgaactc gccgcccgtg gacacgcaat cagcatcgag
      481 ctggacatcg ccgattcggt gacggaagag gcagtcgccc gcttccgtcc cgacctggtg
      541 ctggcgcctt tcctgaaacg ggcgattccc gagtcggtgt ggtcggtcgt gccctgcttg
      601 atcgtccatc ccggcattcc cggcgaccgc ggtccggctg cactcgactg ggcgatccag
      661 cgcggcgaga cgcgctgggg cgtcaccgtg ttgcaggccg aggcggaaat ggacgccggc
      721 ccggtctggg cggcggcgga atttcccatg cgcccggcga gcaagggctc gctctaccgc
      781 aacgaagtca ccgaagcggc ggtgcgcgcc gtgctgaagg cggtcgagcg tttcgctgcc
      841 ggcgatttcc agccgcaacg cgtgcccggt gccgcgcatc cagcgatccg ccaggccgac
      901 cgccagatcg actggacgac gcagcccacc gccgatgtcc tcgcccgact gcgcgccgcc
      961 gacggttttc cgggtgttgc cgacgaactc ttcggccaac cctgtcaact gttcgatgct
     1021 tggccggaag ggcgcctgcg cggcgccccg ggcacgctgc tggcgcgccg cgagacggcg
     1081 ctgtgccgcg cgaccgccga cggcgcggta tggatcggcc acgtcaagaa gccgggcggc
     1141 atcaagctgc cggcgaccgt cgccttcccg gaagcggctg agttgcctga agcgccgctt
     1201 gccggctggt ggcaggccga ccatccgacc tggcaggaca tcgcctggcg cgaggccgac
     1261 ggcgtcggtt tcctgtcctt ccgcttctac aacggggcga tgggcacggc gcagtgccgc
     1321 cggctgaccg aagccttcgg ctgggcgacg acgcggccgg tgcgggtaat cgtcctcgac
     1381 ggcggcagcg acttctggtc gaacggcatc cacctcaacc tgatcgaggc cgccgacagc
     1441 ccggccgacg aatcctgggc caacatcaac gccatcgacg acctggccga ggccattttg
     1501 cgctgccaga cgcaattgac cgtcgccgcc gtcggcggca acgctggcgc cggcggctgt
     1561 ttcctggcgc gcgcctgcga ccgggtctgg gtgcgcgacg gcgtcgtcct caatccgcac
     1621 tacaagaaca tgggcaacct ctacggttcc gagttctgga cctacctgct gccgccgcgt
     1681 gtcggcgccg acggcgcccg ggcgatcatg cagcaccgcc tgccgatgcc ggccgccgag
     1741 gcagtcgcct gcggcttcta cgacgcctgc ctgccggcgc ccggctttgc cgtcgatgtc
     1801 gcccggcggg cgctggaaat ggcgcaggcg gcgaatttcc ccgcgcaact ggcggacaag
     1861 gtggcgcgcc gggccgccga cgaagccgcg aaaccgctgg cgcagtaccg ggaagaggaa
     1921 ctggcggcga tgcggcgcaa tttctatggc ttcgatccgg cctaccacgt cgcgcgttat
     1981 catttcgtcc ataagagccc gcattcgtgg acgccgcggc atctcgcccg gcaccgcgac
     2041 ctcgactgga taatccccac atgaatgccc ccgtgttgcg ccgcctgcct gccgtcctcg
     2101 tcatcgacga cgagttgcgt tcgcaggaag cgttgcggcg cacgctggaa gaggatttcg
     2161 aggtgttctg cgcgtccagc gccgccgaag gctgggaaat cctggagatg gaagaagcca
     2221 gcgccggcat ccgcgtcgtc ctctgcgacc agcgcatgcc cggcaccacc ggcgtgcagt
     2281 tcctcaagga agtccgccag cgctggccgg acatcgtccg catcattctc tccggctaca
     2341 ccgacgccga ggacatcatc agcggcgtca acgaagccgg catctggcag tacctgttga
     2401 agccctggca gccc
//
LOCUS       DAOSDX010000428         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15327_length_2408_cov_2.311942, whole genome shotgun sequence.
ACCESSION   DAOSDX010000428 DAOSDX010000000
VERSION     DAOSDX010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15327_length_2408_cov_2.311942"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLN02_09605"
     CDS             <1..299
                     /locus_tag="PLN02_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI52621.1"
                     /translation="FRDIVLPNCRNGILAGALMVVTLSVGEFNMTWLLHTPFTKTLPV
                     GLADAYASLRLEIGSAYTLIFFLLITPLLLTLQALARPRRQSFAAPDNTIENAS"
     gene            299..808
                     /locus_tag="PLN02_09610"
                     /pseudo
     CDS             299..808
                     /locus_tag="PLN02_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1019..1333
                     /locus_tag="PLN02_09615"
     CDS             1019..1333
                     /locus_tag="PLN02_09615"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOI52622.1"
                     /translation="MNRLSGHADGEVFRCAGGYVAWPALPAGAQEILFRPEDTRIAAD
                     GEAATLHGTIGAAFFCGNHLRLFVEVGEARPLVLETPARREYRRGEPVALAIETHGLM
                     VL"
     gene            1336..2151
                     /locus_tag="PLN02_09620"
     CDS             1336..2151
                     /locus_tag="PLN02_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOI52623.1"
                     /translation="MLIAQLTDTHIKTAGRLAYRRVDTAAMLAAAVDALRRLDPQPDL
                     VVITGDLVDLGNAEEYARLRALLAPLAQPLLVVPGNHDARDALRQAFADGGYLPASGF
                     LHYAVDDHPLRIIGLDTLIPGQGGGELCAERLAWLARTLAARPAAPTLILMHHPPFAT
                     GIGHMDRIGLRGRQAFAALLAQHPQVQRVLCGHLHRTIHAVVGGRAVLTAPSTAHQVA
                     LDLREQAPSAFRMEPPGFMLHRWADDMLVSHVANIGDFAGPFPFFDENGALID"
     gene            2167..>2408
                     /locus_tag="PLN02_09625"
     CDS             2167..>2408
                     /locus_tag="PLN02_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOI52624.1"
                     /translation="MHLALFDLDHTLLDGDSNSLWLSYLVAAGQLPATALHRQAEEYA
                     RYEAGILDIAAYLDFHLSLLAGRPLAKWLPWRERFVA"
BASE COUNT          367 a          880 c          755 g          406 t
ORIGIN      
        1 gtttccgcga catcgtcctg cccaactgcc gcaacggcat cctggccggc gcgctgatgg
       61 tggtgacgct gtcggtcggc gaattcaaca tgacctggct gctccacacg ccattcacca
      121 agacgctgcc ggttggcctg gccgatgcgt acgcctcgct gcgtctggag atcggcagcg
      181 cctacacgct gatcttcttc ctgctcatca ccccgctgct gctcaccctg caggcgctcg
      241 cccggccacg ccggcagtcg ttcgccgcgc cggacaacac tatcgagaat gcttcatgat
      301 gcaaaccgtc cccatcgccc ttgaaaactg tgccaagacc tttgccgacg gcacccgcgc
      361 gctggaagcc gtcaacctgg aaatcgccgc tggcgagacc gtcgtcctgc tcggcccttc
      421 cggttgcggc aagaccacca cgctgcgcct gatcgccggg ctggaacagc ccgatgccgg
      481 cggccgcgtc cgtttcggcg ccgacgacgt gaccgcgctg ccgatcgaaa agcgtaacgt
      541 cggcatggtc ttccagtcat acgcactgtt tcccaacatg acggtggccg agaacatcgc
      601 ttacggcctg cgcctgcgcc gccagtcgac cgccgaacag cgcgcccggg tcggcgaaat
      661 gctcgccctg atgcgcatcg agcgccttgc cgagcgcagc atcgaccagt tgtccggcgg
      721 ccagcgccag cgtgtcgcgc tggctcgggc gctggctgtg cagccgcgcg ttttattgct
      781 cgacgagccg ttgaccgcgc tcgacgccaa gttgcgcgac gtgctgcgct tcgaaatcga
      841 cagcctgctg cgcgagctgg gcatcacggc gatctacgtc acgcacgacc aggccgaagc
      901 gatggcgctg ggcgaccgca tcgtcgtcat ggaccatggg cgcatcgccc aggtcggcgc
      961 gccgcgcgag atctaccagc agccggcgtc gcgctacgtc gccgaattca tcggcacgat
     1021 gaaccgcctg agcggccatg ccgacggcga agtcttccgc tgtgccggcg gctatgtcgc
     1081 ctggccggca ctgcccgccg gggcgcagga aatcctcttt cgccccgaag atacgcggat
     1141 cgccgccgac ggcgaggcgg cgacgctgca tgggacgatc ggcgctgcct ttttctgcgg
     1201 caaccatctc cgcctcttcg tcgaagtcgg cgaagcccgg ccgctggtcc tggaaacgcc
     1261 ggcccggcgc gagtaccggc gtggcgagcc ggtcgcgctg gcgatcgaaa cccacggcct
     1321 gatggttctg taaacatgct catcgcacaa ctgaccgata cccacatcaa gaccgccggc
     1381 cgcctggcct atcggcgggt cgatacggcg gcgatgctgg ccgccgccgt cgacgccttg
     1441 cgccggctcg atccgcagcc agacctggtg gtcatcaccg gcgacctggt cgatctcggc
     1501 aacgccgagg aatacgcccg cctgcgcgcc ctcctggcgc cgttagcgca accgctgctg
     1561 gtcgttcccg gcaaccacga cgcgcgcgac gcgctgcgcc aggctttcgc cgacggcggc
     1621 tatctgccgg ccagcggttt cctgcattac gcggtcgacg accatccgct gcgcattatc
     1681 gggctcgaca ccctgattcc cggtcagggc ggcggcgaac tgtgcgccga acgcctcgcc
     1741 tggctggcgc gcaccttggc cgcgcgcccg gcagcgccga cgctgatcct gatgcaccat
     1801 ccgccgttcg ccaccggcat cggccacatg gatcgcatcg gcctgcgcgg ccggcaggcc
     1861 ttcgccgcgc tgctggcgca acacccgcaa gtgcagcggg tgctgtgcgg ccacctgcac
     1921 cggacgatcc acgccgtggt cggcgggcgg gcggtgctga ccgcgccgag caccgcgcac
     1981 caggtcgcgc tcgacctgcg cgaacaggcg cccagcgctt ttcgcatgga gccgcccggc
     2041 ttcatgctgc accgctgggc ggacgacatg ctggtcagcc atgtcgccaa tatcggcgat
     2101 ttcgccggtc cctttccctt ctttgacgag aacggcgcgc tcatcgactg agcgcgccgg
     2161 ataaacatgc atctggcact tttcgatctt gatcacacgc tgctcgacgg agacagcaac
     2221 agcctctggc tgtcctacct ggtggcagcc gggcaattgc ccgccaccgc cctgcaccgc
     2281 caggccgagg aatacgcgcg ctacgaagcg ggcattctgg acatcgccgc ctacctggat
     2341 tttcatcttt cgttgctggc tggacggccg ctggcgaaat ggttgccctg gcgcgagcgc
     2401 ttcgtcgc
//
LOCUS       DAOSDX010000429         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15330_length_2407_cov_3.608844, whole genome shotgun sequence.
ACCESSION   DAOSDX010000429 DAOSDX010000000
VERSION     DAOSDX010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15330_length_2407_cov_3.608844"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2407)
                     /locus_tag="PLN02_09630"
     CDS             complement(<1..>2407)
                     /locus_tag="PLN02_09630"
                     /inference="COORDINATES: protein motif:HMM:NF023874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HOI52625.1"
                     /translation="AEFGRNEQSLILRTRARTENNCVARDIVRIGDRLLFGYNVFIGL
                     RKETAVGDVFALYRLAEAGGGDELEALPLAGSFLEDQRFVSDFRELHAYYKQATLIQL
                     RVTQDKLLAAFQIGQQLHDLRVFRWSLENDGSLRYIDNRGERDMALPPSHDFEWTPTR
                     REDQVGGRHPHVNILDTVFVETIGGDLTVKIENNTESGLGIYSEAVEDKTQSLDDAEV
                     AYARLGLLILLRIKPYREQTVRYLVYNTRTQSVTRIDAIGVSCIQLPEDHGIIFPGGY
                     YLQSGEHKRFDLPEAMLDNLRFKRVLRSPNGEDVLYVFYQPGLGHYALFTYNLIDKTL
                     GTPILGNGYARFPDGRILVFQADGGEPTRVHPMQLWQTPFCSEEHAGQQSAGQGFFGR
                     IGNAELVRGISELNAIARAVREQVPTRTAYEDLIRQCSRAQDAYFWIGEAEAGGLGDE
                     LRAIGEGARLTLAEFEKVDSIRRETAQALAAAETEQRELLTRIAGMLWRSSDEFVSAL
                     NLLRERRGRLQTLKELRYADLPRIEAMDQALAEEQNRVGERAMQFLATPDAFAGQRHL
                     LDALAARISQAATAQALEQILGELDAVAGGLDLLSEQLGNLPGGDAVLRTDILDRISV
                     LYAETNRLRAEARNRRRNLGSAEAAAEFGAQFKLFGQTVESALEFADTPEKCDEALTR
                     LLARLEELEGRFAEQENFLADIASQREAVYEALSARRQSLLDARQRRVRSLNEAAARI
                     LDGIPRRVAKFQEIAEVHAYFAADPLIGKLQKLVEDLRALGDGVAADDLDTRIKTARD
                     QALR"
BASE COUNT          361 a          731 c          873 g          442 t
ORIGIN      
        1 gcgcagggct tggtcgcggg cggtcttgat gcgcgtgtcg aggtcgtcgg cggcgacgcc
       61 gtcgcccagt gcacgtaggt cttcgaccag cttctgcagt ttgccgatca agggatcggc
      121 ggcgaaatag gcgtgaactt cggcgatttc ctggaacttg gcgacgcgcc ggggaatgcc
      181 gtcgaggatg cgggcagcgg cctcgttgag cgaacggacg cggcgctggc gggcgtcgag
      241 caggctctgg cgacgcgccg acagggcttc gtagacggct tcgcgctggc tggcgatgtc
      301 cgcgaggaag ttctcctgtt cggcaaagcg cccttccagt tcctccaggc gggcgagcag
      361 gcgggtcagc gcttcgtcgc atttctccgg tgtgtcggcg aattccagcg cgctttcgac
      421 ggtctggccg aagagcttga actgggcgcc gaattcggcg gcggcttcgg cgctgcccag
      481 gttgcgccgc cgattgcggg cttccgcgcg caggcggttg gtctcggcgt aaagcacgga
      541 aatgcggtcg aggatgtcgg tgcgcaggac cgcatcgccg ccgggcaggt tgcccaactg
      601 ttcgctcaac aggtcgagac cgccggcgac ggcgtcgagt tcgccgagga tctgttcgag
      661 cgcctgagcc gtcgccgcct gcgagatgcg agcggcgagc gcgtcgagca ggtggcgctg
      721 accggcgaag gcgtccggcg tcgcgagaaa ctgcatggcg cgctcgccga cccggttctg
      781 ttcttcggcc agcgcctgat ccattgcctc gatgcgcggc agatcggcgt agcgcagctc
      841 cttcagcgtc tgcaagcggc cgcgccgctc gcgcaacaag ttgagagcgc tgacgaattc
      901 gtccgaactg cgccacagca tgccggcgat ccgggtcagc aactcgcgct gttcggtctc
      961 ggcggcagcc aaggcctgcg cggtctcgcg gcggatgctg tcgacctttt cgaactcggc
     1021 cagggtcagg cgggcacctt cgccaatcgc ccgcagctcg tcgccgagtc cgccggcttc
     1081 tgcttcgccg atccagaaat aggcatcctg ggcgcggctg cactggcgga tcaggtcttc
     1141 gtaggcagtg cgggtcggca cctgttcgcg cacggcgcgg gcgattgcgt tcaattcgga
     1201 gatgccgcgc accagttcgg cgttgccgat gcggccgaag aaaccctgac cggcgctctg
     1261 ctggccagcg tgttcttcgc tgcagaacgg cgtctgccac aactgcatcg gatgcacccg
     1321 ggtcggctca ccgccgtcgg cctggaaaac caggatgcgg ccgtccggga aacgggcata
     1381 gccgttgccg aggatcggcg tgccgagggt cttgtcgatc aggttgtagg tgaacagcgc
     1441 gtaatggccg aggccgggct ggtagaagac gtagagcacg tcttcgccgt tcggcgagcg
     1501 caggacgcgc ttgaagcgca ggttgtcgag catcgcttcc ggcaggtcga aacgcttgtg
     1561 ctcgccggac tgcaggtagt agccaccggg gaagatgatg ccgtggtctt ccggcaactg
     1621 gatgcaggag acgccgatgg cgtcgatgcg ggtgacgctt tgcgtgcgcg tgttgtagac
     1681 caggtagcgc accgtctgct cgcggtaggg cttgatgcgc agcaggatca gcaggccgag
     1741 ccgggcgtag gcgacttcgg cgtcgtcgag cgactgcgtc ttgtcctcga ccgcttcgct
     1801 gtagatgccg agcccgctct cggtgttgtt ttcgatcttg accgtcaggt cgccgccgat
     1861 ggtttcgacg aagaccgtgt cgagaatgtt gacgtgcgga tggcggccgc cgacctggtc
     1921 ttcgcgccgg gtcggcgtcc attcgaaatc atggctcggc ggcagcgcca tgtcgcgctc
     1981 gccgcggttg tcgatgtagc gcaggctgcc gtcattttcc aggctccagc ggaagacgcg
     2041 caggtcgtgc agttgctggc cgatctggaa ggcggcgagc agcttgtcct gggtgacgcg
     2101 caactggatc agcgtcgcct gcttgtaata ggcgtgcagt tcgcggaaat cggaaacgaa
     2161 gcgctggtcc tcgaggaagc tgccggccag cggcagggct tcgagttcgt cgccgccgcc
     2221 cgcctcggcc agccggtaga gggcgaagac gtcgccgacc gcggtttcct tgcgcaggcc
     2281 gatgaagacg ttgtagccga agagcaggcg gtcgccgatg cggacaatgt cgcgggcgac
     2341 gcagttgttc tcggtgcggg cccgggtgcg caggatcagc gactgctcgt tgcggccgaa
     2401 ttcggca
//
LOCUS       DAOSDX010000430         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15413_length_2399_cov_2.806314, whole genome shotgun sequence.
ACCESSION   DAOSDX010000430 DAOSDX010000000
VERSION     DAOSDX010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15413_length_2399_cov_2.806314"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..625)
                     /locus_tag="PLN02_09635"
     CDS             complement(77..625)
                     /locus_tag="PLN02_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52626.1"
                     /translation="MEFRDLRGKVVLTAFSVFFCCMTLGCSSKTAEEKGKEMASEKID
                     LVKGVGAALEEKGGAAAESVTSGLGSIVSGVKKGVAKSDKRVVNDASLAAAGFQVTAV
                     QETPPGTELESRGIDAYLVAERKVAGLLKVTVYDVLDKEIGRTKIPFALDAEQGKYFR
                     IPLEQQIEFGKIEKLSFVFQAS"
     gene            complement(724..1041)
                     /locus_tag="PLN02_09640"
     CDS             complement(724..1041)
                     /locus_tag="PLN02_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HOI52627.1"
                     /translation="MSFAENLKKLPGITHLAALNLLDADGNVVASIENKAGSQGSLAV
                     YNHLAQTYGSINAEAAKKGIEIFAEHGEDERANPGKHPNIARLLQIEAGAPALRVKHV
                     FAV"
     gene            complement(1053..1325)
                     /locus_tag="PLN02_09645"
     CDS             complement(1053..1325)
                     /locus_tag="PLN02_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52628.1"
                     /translation="MACPDCGGESPDGAAVCPNCATLRRIARLRVSSSALKGGVGGLA
                     LGLLLVTVLLAQGEHAVSVKALLLALPLVLAALGAVIGWRRASSSS"
     gene            complement(1325..>2399)
                     /gene="purB"
                     /locus_tag="PLN02_09650"
     CDS             complement(1325..>2399)
                     /gene="purB"
                     /locus_tag="PLN02_09650"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOI52629.1"
                     /translation="WIKGNTKGNAEVVKVSEFFHFACTSEDINNLSHALMCQGAREQA
                     MLPTLDQVIEKLRELAHAYADIPMMSRTHGQPATPSTMGKEIANVAYRLQRARARIAQ
                     VELLGKINGAVGNYNAHLAAYPGYDWEGFAKKFVESLGLTFNPYTIQIEPHDALAELF
                     DAFARANSILVDLDRDVWGYISLGFFKQKVKAGEIGSSTMPHKVNPIDFENSEGNLGL
                     ANAMLKFLAEKLPISRWQRDLTDSTVLRNMGVGLGYTLLGYDSLLKGLGKLEINADLM
                     KADLDANWELLAEPIQTVMRRYAVANAYEKLKELTRGTRVSRDGMQAFVSTLDIPEAA
                     KAELLKLTPWDYTGKAAELAKRI"
BASE COUNT          434 a          742 c          758 g          465 t
ORIGIN      
        1 gaaaacagcg gttcaacaac caagcacgcc cgcgcgtcag gcgcggaaaa cagcgcatca
       61 gcaagaaaat caaaaatcag gaagcctgaa acacaaagga cagcttttca atcttcccga
      121 attcgatctg ctgctccagc ggaatacgaa aatacttccc ttgctccgca tccagcgcaa
      181 acgggatctt cgtcctgccg atctccttgt ccagcacgtc gtacaccgtg accttgagca
      241 accctgccac cttccgttcg gcgacgagat aggcgtcgat gccgcgactc tcgagttccg
      301 tccccggcgg cgtttcctgg accgccgtca cttgaaaccc ggccgccgcc agcgacgcat
      361 cgttgacgac acgcttgtcg gatttcgcga cgcctttctt gaccccgctt acgatgctgc
      421 caaggccgct ggtcacggat tcggccgctg cgcctccctt ttcttcgagc gcggcgccga
      481 cccctttgac gagatcgatt ttctccgatg ccatttcctt gcccttttct tccgctgtct
      541 tcgatgaaca accaagcgtc atgcagcaaa aaaacaccga aaacgcggtc agaacgacct
      601 tgccccgtag atcacgaaat tccacactgc ctctccctga taaaaattat cgaatcggca
      661 ttatgaaata aaacccggga tcagggatgc accgcggaca tcaatccccg ggcgccgcgg
      721 ctatcacacc gcgaacacgt gcttcacccg caaggccggc gcgccggcct cgatctgcag
      781 caggcgggcg atgttcgggt gcttgccggg gttggcgcgc tcgtcttcgc catgctcggc
      841 aaagatttcg atgcccttct ttgcggcttc ggcgttgatc gaaccgtagg tctgggcgag
      901 gtggttgtag acggcgagcg agccctggct gccggccttg ttttcgatgc tggcgacgac
      961 attgccgtcg gcgtcgagca ggttgagcgc ggcaaggtgg gtgataccgg ggagtttctt
     1021 gaggttttca gcgaaagaca tggcaatcgg gatcaggaag aggaagaagc gcgccgccag
     1081 ccgatcaccg cgcccagcgc ggcgaggacg aggggcagcg cgagcagcag cgccttgacg
     1141 gaaaccgcgt gctcgccctg ggccagcagc acggtaacca gcagcagtcc cagcgccaga
     1201 ccgccgacac cgcccttcaa cgcgctgctg ctcacgcgca ggcgggcgat gcgacgcagg
     1261 gtggcgcaat tcgggcagac ggcggcgccg tccggcgatt cgccgccgca gtcgggacag
     1321 gccatcagat gcgcttggcc agttcggcgg ccttgccggt gtaatcccac ggggtcagct
     1381 tgagcagctc ggccttggcg gcttccggaa tgtccagcgt cgagacgaaa gcctgcatgc
     1441 cgtcgcgcga gacacgggtg ccgcgggtca gctccttcaa cttttcgtag gcgttggcca
     1501 cggcgtagcg gcgcatcacg gtctgaatcg gctcggcgag cagttcccag ttggcgtcga
     1561 ggtcggcctt catcaggtcg gcgttgattt ccagcttgcc cagtcccttc aacagcgagt
     1621 cgtagccgag cagcgtgtag ccgaggccga cgcccatgtt gcggagcacc gtcgaatcgg
     1681 tcaggtcgcg ctgccagcgc gagatcggca gcttctcggc gaggaacttg agcatcgcgt
     1741 tggccaggcc gaggttgcct tcggagtttt cgaagtcgat cggattgacc ttgtgcggca
     1801 tcgtcgacga gccgatttcg ccagccttga ccttctgctt gaagaagccg agcgagatat
     1861 agccccagac gtcgcggtcg agatcgacca ggatgctgtt ggcgcgggcg aaggcgtcga
     1921 acagctcggc cagcgcgtcg tgcggctcga tctggatggt gtaggggttg aaggtcaggc
     1981 cgagcgattc gacgaacttc ttggcgaagc cttcccagtc gtagcccggg taggcagcga
     2041 ggtgggcgtt gtagttgccg acggcgccgt tgatcttgcc gagcagttcg acctgagcga
     2101 tgcgggcgcg ggcgcgttgc aggcggtagg cgacgttggc tatttccttg cccatcgtcg
     2161 acggcgtcgc cggctggccg tgcgtgcggc tcatcatcgg gatgtcggcg taggcatggg
     2221 ccagttcgcg cagcttctcg atgacctggt ccagcgtcgg cagcatcgcc tgttcgcggg
     2281 cgccctggca catcagcgcg tgcgacaggt tgttgatgtc ttccgaggtg caggcgaagt
     2341 ggaagaactc gctgaccttg accacttcgg cgttaccctt ggtgttgccc ttgatccag
//
LOCUS       DAOSDX010000431         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15420_length_2398_cov_3.604353, whole genome shotgun sequence.
ACCESSION   DAOSDX010000431 DAOSDX010000000
VERSION     DAOSDX010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15420_length_2398_cov_3.604353"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLN02_09655"
     CDS             complement(<1..657)
                     /locus_tag="PLN02_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOI52630.1"
                     /translation="MAAGSMRLQNLLGRLNVVQMAAPILILMVLSMMVLPLPPFILDL
                     FFTFNIAIAVLVLMVAVNTQKTLDFSVFPTVLLVTTLLRLSLNVASTRVVLLEGHNGP
                     DAAGKVIEAFGHFLVGGNFAVGIIVFMILVVINFIVITKGAGRVAEVSARFTLDAMPG
                     KQMAIDADLNAGLIGEEEARRRRSDISRESEFYGSMDGASKFVRGDAIAGIVIMLLNI
                     V"
     gene            complement(658..1791)
                     /gene="flhB"
                     /locus_tag="PLN02_09660"
     CDS             complement(658..1791)
                     /gene="flhB"
                     /locus_tag="PLN02_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOI52631.1"
                     /translation="MAEDSDLEKTEEPTGRRIEQAREKGQIPHSRELGTFTVLIVAAM
                     TLWMMGGWFLDRSLALGRKAFTPEIHHLLEPAMIVPRMVELFADTFFVFSPLFGLLLV
                     AAILPPFFLNAWVFAPKALVPDLQRMNPLSGLGRMFSWNSLMELGKAVLKATLVGGVA
                     VLLISRQRDAIFGLLAEPLESGLAHAGDMLMFSFLMLVLTLLIVVAADVPFQLWQYYH
                     KLKMTKDEVKQEMKEMMGDPMVKGRIRSLQMQAARRRMMQAVPQADVIVTNPTHYAVA
                     LAYKAGMAAPKVVAKGTGVIALKIRELAAEHAVPTLEAPPLARALYKHGDLDKEIPGG
                     LYAAVAEVLAYIYQLARWRETGGAYPVPPQQLPVPPELVPEAA"
     gene            complement(1975..>2398)
                     /locus_tag="PLN02_09665"
     CDS             complement(1975..>2398)
                     /locus_tag="PLN02_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI52632.1"
                     /translation="LLVLLGDQPFALPLSLVREILPIDQQRIQEVGGKETLVVRGEVL
                     PVISLAKLLGWEIVRPPEYGVFMQTAERSFILGVDSFAGRDDAVIKSLEDFRPKGVAG
                     VTTLSNGQIVLILDMKELLADYGQHAEMGTAIRNFEFS"
BASE COUNT          517 a          743 c          717 g          421 t
ORIGIN      
        1 gacgatgttg agcagcatga tgacgatgcc ggcaatggcg tcgccgcgga caaatttcga
       61 cgcaccgtcc atcgaaccgt agaactcgga ttcgcgggag atgtcgctgc gccgccgccg
      121 ggcctcttct tcgccgatca ggccggcgtt gaggtcggcg tcgatcgcca tctgcttgcc
      181 cggcatcgca tcgagagtga agcgcgcgga aacttcggcg acgcgcccgg cgcccttggt
      241 gatgacgatg aagttgatca ccaccagaat catgaaaacg atgataccga ccgcgaagtt
      301 gccgccgacc agaaaatgcc cgaaggcctc gatcaccttg ccggccgcat ccggaccgtt
      361 atgcccttcg agcagcacga cgcgggtgga ggcgacgttg agcgacaggc gcaacagcgt
      421 cgtcaccagc aggacggtcg gaaaaaccga gaaatccagc gttttctggg tgttcaccgc
      481 aaccatcagg accagcacgg cgatcgcgat gttgaaggtg aagaagagat cgaggatgaa
      541 cggcggcaac ggcaggacca tcatggacag caccatgagg atcaggatcg gcgccgccat
      601 ctggacgacg ttcagccgtc cgagcaggtt ttgcagacgc atcgaaccgg ccgccattta
      661 cgcagcctcc ggcacgagtt cgggcggcac cggcaactgc tgcggcggca ccggataggc
      721 accccccgtt tcgcgccagc gcgccagttg gtagatgtag gcgagaactt cggcgaccgc
      781 ggcgtaaagc ccgcccggaa tctccttgtc gagatcacca tgcttgtaca gggcgcgtgc
      841 cagcggcggc gcttccagcg tcggcaccgc atgctcggcg gccagttcgc ggatcttcaa
      901 ggcgatcacg ccggttccct tggccaccac tttcggcgcc gccatgccgg ccttgtaggc
      961 gagggcgacg gcgtagtgcg tcgggttggt gacgatcacg tcggcctgcg gcaccgcctg
     1021 catcatccga cgccgcgcag cctgcatctg caggctgcgg atgcgcccct tgaccatcgg
     1081 gtcgcccatc atttccttca tttcctgctt gacctcgtcc ttggtcatct tcagcttgtg
     1141 gtagtactgc cagagctgga agggaacatc cgccgcgacc acgatgagca gcgtgagaac
     1201 gagcatcagg aaggaaaaca tcagcatgtc gccggcgtgc gccagtccgc tttccagcgg
     1261 ctcggccagg agaccgaaga tcgcgtcgcg ctgccgcgaa atcagcagca cggcgacgcc
     1321 accgaccagc gtcgccttga gtaccgcctt gccgagctcc atcaggctgt tccaggagaa
     1381 catccgaccc agaccgctca gcggattcat gcgctgcaga tcgggaacca gggccttcgg
     1441 cgcgaacacc caggcattga ggaagaacgg cggcaggatc gccgccacca gcagcaggcc
     1501 gaatagcggc gagaaaacga agaaggtgtc ggcaaacaat tcgaccatcc gcgggacgat
     1561 catcgccggc tcgagaaggt ggtgaatttc cggggtaaag gccttgcgcc ccaaagccag
     1621 actgcggtcg agaaaccagc cacccatcat ccaaagcgtc atggcggcaa cgatgagcac
     1681 ggtgaaagta ccgagctcgc gcgaatgggg aatctgccct ttttcgcgcg cctgctcgat
     1741 acgcctaccg gttggctctt cagttttctc gagatcgctg tcttcagcca tgaccattat
     1801 ctgtacaggg tatggctatt atagaaaaaa accaaagaaa ttaaataggt agcgcgtctt
     1861 ttttaatcag tttcgaggta aacggcgcaa gccaatgaca aatagcaaaa cccaaaaaaa
     1921 gggaggaggc ctcagcctcc tccctaactc gttgaccggc aaggaaatcg tcgttcagga
     1981 gaattcgaaa ttgcggatcg ccgttcccat ttcagcgtgc tggccgtaat cggcaagcaa
     2041 ctccttcatg tcgaggatga gcacgatctg tccgttcgac agcgtggtca cgcccgccac
     2101 ccccttcggc cggaaatctt ccagcgactt gatgacggca tcgtcgcgac cggcgaagct
     2161 gtccactccc aggatgaagc tacgttccgc ggtctgcatg aagaccccgt attcgggtgg
     2221 tctgacgatt tcccagccga gcagcttggc cagcgaaatc accggcagca cttcgccgcg
     2281 taccaccagg gtttccttgc cgccgacttc ctggatgcgt tgttgatcga tcggcaggat
     2341 ttcccgaacc agcgacagcg gcagggcgaa cggctgatcg ccgagcagca ccagcagg
//
LOCUS       DAOSDX010000432         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15450_length_2395_cov_2.085897, whole genome shotgun sequence.
ACCESSION   DAOSDX010000432 DAOSDX010000000
VERSION     DAOSDX010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15450_length_2395_cov_2.085897"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLN02_09670"
     CDS             complement(<1..320)
                     /locus_tag="PLN02_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOI52633.1"
                     /translation="MTDEQLLRYSRHILLDALGIEGQQRILDSHALIVGAGGLGSPAA
                     LYLASAGIGRITLVDDDVVDLTNLQRQILHTEARVGMAKAESGRLALAALNPAVEVVP
                     LQSRL"
     gene            complement(317..718)
                     /locus_tag="PLN02_09675"
     CDS             complement(317..718)
                     /locus_tag="PLN02_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52634.1"
                     /translation="MSSTDLRKKREILFAKFPPGQVPEAADDLKKVEEVDVEPRYEKR
                     ALGVAYDLQQHTLAELEDRLVDHGYHLDSTLMSKLTRALIHYVEETQLHNMASPEKRL
                     KPSPEEAYVKAWEHRPHGDHDDTPPEWREYK"
     gene            complement(743..2125)
                     /locus_tag="PLN02_09680"
     CDS             complement(743..2125)
                     /locus_tag="PLN02_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOI52635.1"
                     /translation="MSKIKTLSLAVGGFVAGALITLQLPAFAEKDAKIGLPIEDLRTF
                     AEVYSAIKQGYVEPVEDKKMIANAIAGMLGNLDPHSAYLDADSFKDLQVGTQGEFGGL
                     GIEVGMEDGLVKVVSPIEGTPAYRAGVKAGDLIFKLDDTPVKGLSLSDAVKKMRGKPK
                     TSIRLGILRKGEAKPLEITLVREVIKVQSVKSKLIEPGYAWVRITQFQETTVADLAKH
                     LGKLYKEGELKGLVLDLRNDPGGLLNGAIGVSAAFLPPEVKVVSTDGRTEDAKQEFLA
                     RPRDYLRGTGEDPLRSLPVAVKKVPMVVLVNGGSASASEIVAGALQDHKRAAIVGTQS
                     FGKGSVQSVLPLPGNTAIKLTTARYFTPSGRSIQAKGITPDIVVDETPNGNGPARLRE
                     ADLERHLEDKQDAAKKEDAKPQPSKPAAKEEHDDEAMPARLEYASKSDYQFQQALNIL
                     KGVQFMQGKQ"
     gene            complement(2122..>2395)
                     /locus_tag="PLN02_09685"
     CDS             complement(2122..>2395)
                     /locus_tag="PLN02_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOI52636.1"
                     /translation="SPVKAIASGRVVFSEWMRGFGNLLIVDHGDAYLSIYGNNDSLLK
                     QVGQAVDGGETIATVGNTGGNPESGLYFEIRHKGNPIDPLSWASLK"
BASE COUNT          346 a          725 c          818 g          506 t
ORIGIN      
        1 agccggcttt gcagcggcac cacttcgacc gcgggattca gtgcggctag cgccagacgg
       61 ccggattcgg ccttggccat gccgacgcgc gcttcggtgt ggagtatctg tcgctgcagg
      121 ttggtcaggt cgacgacgtc gtcgtccacc agggtgatcc ggccgatgcc ggccgaggcg
      181 agatagagcg ccgctggcga gccgagaccg ccggcgccga cgatcagcgc gtggctgtcg
      241 aggatgcgtt gctggccttc gataccgagc gcgtcaagga ggatgtgccg gctgtagcgg
      301 agaagctgct cgtcggtcac ttgtactcgc gccactccgg cggggtgtcg tcgtggtcgc
      361 cgtgcggccg gtgttcccag gccttgacgt aggcttcctc gggcgagggc ttgaggcgct
      421 tctcggggga ggccatgttg tgcaactggg tttcctcgac gtagtggatc agcgctcgcg
      481 tcagcttgct catcagcgtg ctgtcgagat ggtagccgtg gtcgaccagg cggtcctcga
      541 gttcggccag ggtgtgctgc tgcaggtcgt aagcaacgcc gagcgcccgc ttttcatagc
      601 gcggctcgac atcgacctct tccaccttct tgaggtcgtc ggcggcttcc ggtacctgtc
      661 ctggcggaaa cttggcgaac aggatttccc gtttcttcct gaggtccgtg ctgctcatca
      721 tccctcccgc gcggtgatcg gcttactgct taccctgcat gaactgtacg cccttcagga
      781 tattcagcgc ctgctggaac tggtagtcgc tcttgctggc atattcaaga cgggccggca
      841 tcgcttcgtc gtcgtgttct tccttggcgg cgggcttgct cggctggggt ttggcgtctt
      901 ccttcttcgc cgcatcctgc ttgtcttcga gatggcgttc gaggtcggct tcgcgcaggc
      961 gggcaggacc gttgccgttc ggggtctcgt cgacaacgat gtcgggggtg atgcccttgg
     1021 cctggatcga acgcccgctg ggcgtgaagt agcgagccgt ggtcagcttg atcgccgtgt
     1081 tgccggggag cggcagcacc gactgcaccg agcccttgcc gaaggactgg gtgccgacga
     1141 tggcggcgcg cttgtggtcc tgcagggcgc cggcgacgat ttccgacgcc gaggccgagc
     1201 cgccattgac cagcaccacc attggtactt tcttgaccgc caccggcagg ctgcgcagcg
     1261 ggtcttcacc ggtgccgcgc aggtagtcgc gcggccgggc gaggaattcc tgcttggcgt
     1321 cctcggtgcg gccgtcggtc gatacgacct tgacttccgg cggcaggaag gcggcggaga
     1381 cgccgatggc gccgttgagc aggccgcccg gatcgttgcg caggtcgagg acgaggccct
     1441 tgagttcgcc ttccttgtac agcttgccga ggtgcttggc gaggtcggcg accgtggttt
     1501 cctggaactg ggtgatgcgg acccaggcgt agcccggttc gatgagcttg gacttgacgc
     1561 tctgcacctt gatcacttcg cggaccagcg tgatttccag cggcttggcc tcgcccttgc
     1621 gcaggatgcc gaggcggatc gaggtcttcg gcttgccgcg catcttcttg accgcgtcgg
     1681 acaggctgag gcccttgacc ggggtgtcgt cgagcttgaa gatcaggtcg ccggccttga
     1741 ccccggcacg gtaggccggc gtgccctcga tcggcgagac gaccttgacc agaccgtctt
     1801 ccatgccgac ttcgatgccg aggccgccga actcgccttg ggtgccgacc tgcagatcct
     1861 tgaacgaatc ggcgtcgagg taggcggagt gggggtcgag attgccgagc atgccggcga
     1921 tggcattggc gatcatcttc ttgtcttcga ccggttcgac gtagccctgc ttgatcgcgc
     1981 tgtaaacctc ggcgaaggtg cgcagatcct cgatcggtag tccgatcttg gcgtccttct
     2041 cggcgaaggc gggcaactgc agggtgatca gtgcgccggc aacgaagccg ccgacggcga
     2101 ggctcaaggt cttgattttg ctcatttcag gctggcccag gacaaggggt cgatcggatt
     2161 acctttatga cggatttcga agtataaacc ggattctgga ttgcccccgg tgttgccgac
     2221 cgtggcgatg gtttcacccc cgtcgacggc ctggccgacc tgcttgagca gcgagtcgtt
     2281 gttgccgtag atcgacaggt aggcgtcacc atggtcgacg atcagcaggt tgccaaaacc
     2341 gcgcatccac tccgaaaaca ccactcggcc ggaggcgatc gccttgaccg ggctg
//
LOCUS       DAOSDX010000433         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15476_length_2391_cov_3.591610, whole genome shotgun sequence.
ACCESSION   DAOSDX010000433 DAOSDX010000000
VERSION     DAOSDX010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15476_length_2391_cov_3.591610"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1947
                     /locus_tag="PLN02_09690"
     CDS             <1..1947
                     /locus_tag="PLN02_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52637.1"
                     /translation="VSIGFDGLYRQLLGNAALILVVFAAGLWVILRRLGARVESSLRP
                     LDLLDETMAEISIGHLDIRAAPSEIMEFNRLSDGFNQMIAQIRERDHWLATHLGNLEQ
                     IVEQRTRELRQAKEVAEAGSHAKSEFLAMMSHEIRTPMNGVLGMTELLLNTDLTPTQR
                     QFVEAVDRSGRHLLTIINDVLDFSKIEAGHLELEVQDFDLRALLEESVELFSQPASKK
                     GLALMIELPAGRSLKLRGDALRLRQVVANLLNNAIKFTDEGEIVVSLLLLSGEAGSVA
                     FELKVRDSGIGIPQDAQARIFEHFAQADGSTSRRFGGTGLGLTICRQLIEMMGGRISV
                     DSRPGHGSCFTVALSLPIAETPELSADQANEETTLRRVALPSLDEQYLPARLRGRVLL
                     AEDNESNQIVARTHLERFGLQVLLVGNGQQALDLLESEPFDLVLMDCQMPVVDGFAAC
                     AELRRREQRAGLPRVPVVALTANAMRDDRKRCLAAGMDDYLAKPYRGDEMLAVLERWL
                     PRERRKSSGPSVPNGGVEASAGLPIDPAAFDQLRALAPNGASALIRQLIDAYLRGARK
                     HWEACEKAVAGGDLELLAQSCHALKSSSFNVGALRFAGLCREIESLSQEAARDEVQAL
                     YPGLQAEWHAVSDALGVMLAGDAS"
     gene            1944..2321
                     /locus_tag="PLN02_09695"
     CDS             1944..2321
                     /locus_tag="PLN02_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52638.1"
                     /translation="MSQRCLRVLAADDDPTMAILLRAVLQAPAFELVVAGNGADALRA
                     ASAQDAFDIALLDVEMPEVDGLQVAAQLRRQFGERLCIVLLTGREDAAFVAAQQSLRA
                     QYLPKPVDWTQLPQRLRDWSAER"
BASE COUNT          424 a          677 c          817 g          473 t
ORIGIN      
        1 gtgagtatcg gattcgatgg cctgtatcgg caattgctcg gtaacgcggc actgattctg
       61 gtcgtgttcg ctgcggggct ctgggtgatt cttcgccgtc tgggagctcg ggtcgaaagc
      121 agcttgcggc cgctcgacct gctcgacgag acaatggcgg aaatctcgat tggtcatctc
      181 gatattcgtg cggcaccgtc cgaaatcatg gagttcaacc gcctgagcga tggtttcaac
      241 cagatgattg cgcagatccg cgagcgcgat cactggttgg cgacccacct gggcaatctc
      301 gagcagatcg tcgaacagcg cacccgcgaa ttgcgccagg cgaaggaggt cgcggaagct
      361 ggcagccacg ccaaaagcga gtttctggcg atgatgagtc acgagatacg gacgccgatg
      421 aacggtgtgc tggggatgac cgagttgttg ctcaataccg acctgacgcc gacgcagcgt
      481 cagtttgtcg aagcggtgga tcgctcgggg cgacatctgc tgacgatcat caacgacgtc
      541 ctcgattttt cgaagatcga ggccggtcat ctcgaactgg aggtgcagga ttttgatctg
      601 cgcgccctgc ttgaagagtc ggtggaattg ttctcccagc cggcaagcaa gaaggggctc
      661 gccctgatga tcgagttacc ggccgggcgc agcctgaagc tgcgcggcga cgccctgcgc
      721 ttgcgccagg tggtcgccaa cctgctcaac aacgcgatca aattcaccga cgaaggcgag
      781 atcgtcgtca gtctgctcct gttgtccggc gaagccggga gtgtggcttt cgaactcaag
      841 gtccgggaca gcggcattgg catcccgcag gacgcgcagg cccggatttt cgagcatttt
      901 gcccaggctg acgggtcgac ctcgcggcgc ttcggtggta ccggtctggg cttgaccatc
      961 tgtcgccaac tgatcgaaat gatggggggg cggatcagcg tcgacagccg gcctggtcat
     1021 ggctcctgtt ttaccgtggc actgagcttg ccgatagcgg aaacccctga actgtcggcc
     1081 gatcaggcga atgaagagac gacgctgcgt cgggtggcgc taccctcgct cgacgagcaa
     1141 tacttgccgg cgcgtttgcg cggacgtgtg ctgttggccg aagacaacga aagcaaccag
     1201 atcgtcgccc gtacccatct cgagcgcttc ggcctgcagg ttctgctggt cggtaacggc
     1261 cagcaggcac tcgatctact ggagagtgaa cccttcgatc tggtgttgat ggattgccag
     1321 atgccggtgg tggatggctt tgccgcctgt gccgagttac gccgccgcga acaacgcgcc
     1381 ggtttgccgc gggtgccggt agtcgcgctc accgccaatg ccatgcgcga cgaccgcaag
     1441 cgctgtctgg cggcaggcat ggacgattat ctggccaagc cttaccgtgg cgacgaaatg
     1501 ctcgccgtgc tcgagcgctg gttgccgcgc gagcggcgca aatcgagcgg gccgagcgtg
     1561 ccgaacggcg gggtcgaggc gtcggccggc ttgccgatcg atccggccgc tttcgaccag
     1621 ctccgtgcct tggcgccgaa cggcgcgagc gccctgatcc ggcaactgat cgacgcttat
     1681 ctgcgcggcg ccaggaagca ttgggaagcg tgcgagaagg ccgttgccgg aggcgatctg
     1741 gaattgctgg cgcagtcctg tcatgccttg aagtcgagta gcttcaatgt cggcgccctg
     1801 cgctttgccg gtttgtgccg ggaaatcgaa tcgttaagcc aggaagcggc gcgcgatgaa
     1861 gtgcaggcgc tttatcctgg cctgcaggcc gaatggcatg cggtgagcga cgccctgggc
     1921 gtcatgttgg cgggggatgc gtcatgagtc agcggtgttt gcgggtattg gcggcggacg
     1981 acgatccgac gatggcgatc ctgttacggg cggtgctgca ggcgccggca ttcgaactcg
     2041 tcgttgccgg caatggcgcg gatgccttgc gcgccgccag cgctcaggat gccttcgata
     2101 tcgccttgct cgatgtcgag atgccggagg tcgatggctt gcaagtggca gcgcagttgc
     2161 gtcggcaatt tggtgagcgt ctgtgcatcg tgctgctgac tgggcgcgag gatgcggcct
     2221 tcgttgccgc acaacagagt ttgcgggcgc aatatctgcc gaaaccggta gactggacgc
     2281 aactgccgca acgcctgcgc gattggtcgg ccgagcgctg atcccgtttt ggccgctggg
     2341 cgccgaagcg ggtacactgc gaaaaaagcg attgtgaaca gatggagggg a
//
LOCUS       DAOSDX010000434         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15480_length_2391_cov_2.724743, whole genome shotgun sequence.
ACCESSION   DAOSDX010000434 DAOSDX010000000
VERSION     DAOSDX010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15480_length_2391_cov_2.724743"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..933
                     /locus_tag="PLN02_09700"
                     /pseudo
     CDS             220..933
                     /locus_tag="PLN02_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            973..1311
                     /locus_tag="PLN02_09705"
     CDS             973..1311
                     /locus_tag="PLN02_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52639.1"
                     /translation="MPGEEVAPGVWVGLNTSVAWESAKLVGPVYLGSNVRIDPGVSII
                     GPAWIGHGSHIRSGTRIERCIVFEYTRIGDAQHLREMIVSPQYCVGRDGVAHYLGEDD
                     SPYRWGDARG"
     gene            complement(1321..>2391)
                     /locus_tag="PLN02_09710"
     CDS             complement(1321..>2391)
                     /locus_tag="PLN02_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOI52640.1"
                     /translation="HRLTAAERRIHILEGRQAILLDIDRVIKVIRESDDPKADLIAHF
                     KLSDIQAEDILEIRLRQLARLEGLKIGEDLAKLREEAAGLNKLLDSESALRDCVAAEI
                     EADAKKYGDDRRTLIEAAERVTLSDAKNVSIPDEPTTLIVSKHGWLRSRSGHNVDPGQ
                     LTFRSGDSLLACLPCRTVNHLILLDTKGRAYSIPAADLPGGKGDGVPATSLADFQDGG
                     KLVLALAVSADKTYLVAGEGGYGFRVKGEDLLSRGKAGKAFLTLDDNEAPAIFQPAPA
                     GGEIAVFTRDGRALVFALDDIRQLPKGRGLKLIDAAPGKSALETIEAVVDGVAGKLKG
                     ERLDNCRGSRGGKGKPLRATRK"
BASE COUNT          369 a          742 c          854 g          426 t
ORIGIN      
        1 cccggtctga gcgacgacga gttctaccgc tcgcgtgtca tccgcgacct cgccgccttc
       61 aagcagcagg ccgacctcat cgtcgccaac cggatgagcg atgaaatcag cgacgtggcg
      121 gccaaggtat atacccgcga cctgttcggt ggcgattgat caatcgcccg gccgccggcc
      181 ggggcgtgac ggaggaagca ggcagcaatg gccaagggaa tgatactggc ggcggggcag
      241 ggaacgcggg tgcgtccgct gaccaagtcg atgccgaaac cgatggtgcc gatcctcggc
      301 aaaccggtga tggaatacct gatcgagcac atggcgcgct acggcgtgcg cgacatcatg
      361 gtgaacgtcg ccttcaacca tcgcaagatc gaaaattact tcggcgatgg tcggcgctgg
      421 ggcgtcaatc ttggctactc gttcgaaggc gcccgcgaac acggggaaat cgtcccctag
      481 gccatgggct cggccggcgg catgcgcaag atccaggact tcggcggctt cttcgacgag
      541 acgacgctgg tcatctgtgg tgacgcgctg gtcgatctcg acatcgcggc ggcgctgcgc
      601 gaacaccgtg agaagggcgc gctggccagc gtggtgacgc tggaagtgcc gttggccgac
      661 gtgccgaact acggcgtcgt ggtcgccgac gacaacgggc gggtgctctc cttccaggaa
      721 aagccgaaac ccgccgaggc gaagtcgcgc ctggcgagca ccggcatcta tatcttcgaa
      781 ccggcggtgc tcgatctgat tccgcccggc cagcccttcg acatcggcag cgaactgttc
      841 ccgctgctgg tcgagcgcgg cttgcccttc tacgcccagc ggcgtccttt caactggatc
      901 gacatcggtc gcttgagcga ttactgggag gtttcgcagc gcgtgctgcg cggcgaggtc
      961 gagcagatgg agatgccggg cgaggaagtt gcgcccgggg tctgggtcgg cctcaatacc
     1021 tcggtggcct gggagagtgc gaagctggtc gggccggtct acctcgggtc caacgtgcgc
     1081 atcgatcccg gcgtctcgat catcggtccg gcgtggatcg ggcacggcag tcacatccgc
     1141 tcgggaacgc gcatcgagcg ctgcatcgtt ttcgaataca cgcgcatcgg cgacgcgcag
     1201 cacctgcgcg agatgatcgt ctcgccgcaa tactgcgttg gccgtgatgg cgtcgcgcac
     1261 tacctgggcg aggacgattc gccctaccgc tggggcgacg cgcgcggctg aggcggtgcc
     1321 ttacttgcgc gtcgcgcgca gcggtttgcc tttgccgcca cggctgccgc ggcagttgtc
     1381 caggcgttcg cccttgagct tgccggcgac gccgtcgacc accgcttcga tggtttccag
     1441 tgcgctcttg ccgggcgccg cgtcgatcag cttgaggccg cgtcccttgg gcaactggcg
     1501 gatgtcgtcg agggcgaaga cgagcgcccg gccgtcgcgg gtgaatacgg cgatctcgcc
     1561 gccagccggg gcaggctgga agatggccgg cgcttcgttg tcgtcgaggg tcaggaaggc
     1621 cttgccggcc ttgccgcgcg aaagcaggtc ctcgcccttg acgcggaagc cgtagccgcc
     1681 ttcgccggcc accaggtagg tcttgtcggc cgagacggcc agggcgagca ccagcttgcc
     1741 gccgtcctga aagtcggcga gcgaggtggc gggaacgccg tcgcccttgc cgccgggcag
     1801 gtcggcggcg gggatcgagt aggcgcggcc cttggtgtcg agcaggatca ggtgattgac
     1861 cgtgcggcag ggcaggcagg cgagcaggct gtcgccggag cggaaagtca gttgccccgg
     1921 atcgacgttg tggccgctac gcgagcgcag ccagccgtgc ttggagacga tcagcgtggt
     1981 cggctcgtcg gggatcgaga cgttcttggc atccgacagc gtgacgcgtt cggcggcttc
     2041 gatcagggtg cggcggtcgt cgccgtactt cttggcatcg gcttcgatct cggcggcgac
     2101 gcagtcgcgc agcgcgcttt cggagtcgag cagcttgttg aggccggcgg cttcttcacg
     2161 cagcttggcc agatcctcgc cgatcttcag gccttccagc cgcgccagtt ggcgcaggcg
     2221 gatttccagg atgtcttcgg cctggatgtc ggagagcttg aagtgggcga tcaggtcggc
     2281 cttggggtcg tccgattcgc ggatcacctt gatcacccgg tcgatgtcga gcaggatcgc
     2341 ctgccggcct tcgaggatgt ggatgcgccg ttcggcggcg gtcagccggt g
//
LOCUS       DAOSDX010000435         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15503_length_2387_cov_3.189537, whole genome shotgun sequence.
ACCESSION   DAOSDX010000435 DAOSDX010000000
VERSION     DAOSDX010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15503_length_2387_cov_3.189537"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2105)
                     /locus_tag="PLN02_09715"
     CDS             complement(<1..2105)
                     /locus_tag="PLN02_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52641.1"
                     /translation="MKRLLAAAGALAAAIGGILWFLLLMATEADTVVFSRNYPLLVGL
                     NVVLALAMLALVGWQLSTLWRDYRAQVFGARLKLRLMLMFGVIAVLPGALVYGVSVQF
                     VTRSIESWFDVRVEKALESGLQLGRSSLDSLLENLSQRARDMADELSVVPETAQRSAL
                     FRLREERGVHTAALFSLSGQLLSSATGDLGNQLPNAPSQAQLRQARAAQGLSGVEGEN
                     ERMFMRVLVPVTSLELFAEPRILQVTQRVPATLALDAEAVQDVYRDYQELQLARQGLT
                     GIYALTLTLTVLVALFSAFALAFWMARRLVAPMYILAEGTQAVAQGDFSQRQAIYSGD
                     ELGVLTQSFNRMTRQLADVRRENDRHRAYLESILANLSAGVLVFDRDFLLRTVNGGAS
                     SILDDDFAGLIGLAPADWPRQQVLAAFIDQHFAGTDEPEWRAQLEIERPNSAPQVLLL
                     RASRLPESSGGGDVVVFDDVTNLIAAQRTAAWGEVARRLAHEIKNPLTPIQLSAERLQ
                     FKLAERLSNGDAEMLSRGTQTIIAQVQAMKRMVDDFRDYARKPVLEVAPLDLNGLLRE
                     VLGLYESSSVHFDSRLGEGLPQVCGDATQLRQIIHNLLRNAEDALEGRADGRVEIVTE
                     VAGRCVRLRITDNGPGFPIELLPRIFEPYVTTKARGTGLGLPIVRKIVDEHQGSIEIS
                     NAPAGGARIDIRLPLVRDK"
     gene            complement(2102..>2387)
                     /locus_tag="PLN02_09720"
     CDS             complement(2102..>2387)
                     /locus_tag="PLN02_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOI52642.1"
                     /translation="STGGLHQSFATLGEALVMLRRLRNWQVIERNDPVAVPGSTYQAA
                     LRLRLDIAQLPRPFQISALGSKDWNLESDWKIWSATLPPPPTPAPVPEDK"
BASE COUNT          435 a          799 c          775 g          378 t
ORIGIN      
        1 tccttgtccc gcaccagcgg caggcggatg tcgatccgcg caccgccggc cggcgcattg
       61 ctgatttcta tactaccttg atgctcatcg acgatcttcc tgacgatggg cagaccgagc
      121 ccggtacccc gggccttggt cgtcacatac ggttcaaaga tgcgcggcag caactcgatc
      181 gggaaacccg gaccgttatc ggtgatgcgc aggcggacgc aacgcccggc cacttcggtg
      241 acgatctcga cccgcccgtc ggcgcgtcct tcgagggcat cctcggcgtt gcgcaacaga
      301 ttgtggatga tctgccgtag ctgggtcgcg tcgccgcata cctgcggcaa accctcaccc
      361 aggcggctgt cgaaatgcac cgacgagctt tcatacagcc cgagcacttc gcgcagcagg
      421 ccgttgaggt cgagtggcgc gacttccagc accggcttgc gggcgtaatc gcggaaatcg
      481 tcgaccatcc gcttcatcgc ctgcacctgg gcgatgatcg tctgcgtacc gcgcgaaagc
      541 atctcggcat cgccgttgct caggcgctcg gccagcttga actgcaggcg ctcggcggaa
      601 agctggatcg gcgtcagcgg gttcttgatc tcgtgcgcca gacgccgcgc cacttcgccc
      661 caggcggcgg tgcgctgggc ggcgatcagg ttggtgacgt cgtcgaagac gacgacgtca
      721 ccgccaccac tgctttccgg caggcgcgag gcgcgcagca aaagcacctg cggcgcactg
      781 ttcggccgct cgatttccag ttgcgcccgc cactccggtt cgtcggtgcc ggcaaaatgc
      841 tggtcgatga aggcggcgag aacctgctgg cgcggccagt ccgccggtgc caggccgatc
      901 aggccggcaa aatcgtcatc aaggatgctg ctcgcgccgc cgttgaccgt acgcaacagg
      961 aagtcacggt cgaaaaccag caccccggcc gagaggttgg ccaggatcga ttccaggtag
     1021 gccctgtggc ggtcgttctc gcgccggacg tcggccagtt gccgggtcat ccggttgaag
     1081 gactgggtca gcacgccgag ttcgtcaccg ctgtagatcg cctggcgctg cgagaaatcg
     1141 ccctgcgcca cggcctgtgt accctcggcc aggatgtaca tcggcgccac cagccggcgg
     1201 gccatccaga aggccagcgc gaaagcgctg aacagcgcga cgagcacggt cagcgtcagc
     1261 gtcagcgcat aaatcccggt caggccctga cgcgcaagtt gcagttcctg atagtcacgg
     1321 tagacatcct ggaccgcttc cgcatcgagc gccagcgtgg ccggcacccg ttgggtcact
     1381 tgcaggatgc gcggttcggc gaaaagctcg agcgaagtca ccggcaccag cacgcgcatg
     1441 aacatccgtt cgttctcgcc ttcgaccccg ctcaggccct gcgccgcacg agcctggcgc
     1501 aactgggcct ggctcggcgc attgggtagc tggttgccga ggtcgccggt ggcgctggac
     1561 aacaactggc cgctgaggga aaaaagcgcc gccgtatgga cgccgcgctc ctcgcgcaga
     1621 cggaacagtg ccgaacgctg ggcggtttcc ggcaccaccg ataattcgtc ggccatgtcg
     1681 cgcgcccgct gggagaggtt ctcgagcagc gaatcgagcg aggagcgccc cagttgcagg
     1741 ccggactcca gcgccttttc gacccgcacg tcgaaccagc tttcgatcga gcgcgtcacg
     1801 aactgcaccg aaacgccata caccagcgcc cccggcaaca ccgcgatgac gccgaacatc
     1861 agcatcagac gcagcttgag acgggcaccg aagacctgcg cccgataatc gcgccacaag
     1921 gtgctcagct gccagccgac cagtgccagc atggccagcg ccaggacgac gttgagcccg
     1981 accagcagcg ggtagttgcg cgagaagacg acggtgtcgg cttccgtcgc catcagcagc
     2041 aggaaccaga gaatgccgcc gatggcggca gccagcgccc cggcggcagc gaggagtcgc
     2101 ttcacttatc ctccggcacg ggagcagggg taggcggggg cggcagggtc gccgaccaga
     2161 ttttccagtc cgactcgaga ttccagtcct tgctgccgag cgcgctgatc tggaagggac
     2221 gcggtaactg ggcaatgtcc agccgcaggc gcaaggccgc ctggtaggtg ctgccgggaa
     2281 ctgccaccgg gtcgttgcgc tcgatcacct gccagttgcg caaacgacgc agcatgacca
     2341 gggcctcgcc cagggttgcg aaggactggt gcaagccacc ggtgctc
//
LOCUS       DAOSDX010000436         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15504_length_2387_cov_3.066895, whole genome shotgun sequence.
ACCESSION   DAOSDX010000436 DAOSDX010000000
VERSION     DAOSDX010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15504_length_2387_cov_3.066895"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLN02_09725"
     CDS             <1..649
                     /locus_tag="PLN02_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOI52643.1"
                     /translation="HGEVVLDLCFHCHGVWFDEFESVQITPGGVIELFRLIHEYREAP
                     RLPLRDVLRCPRCNERLLQGLDVSKHGGHFNYHRCLQKHGRFTTFAQLMIEKGFVRQL
                     SPAEIDELASKVSIVRCTGCGAPIDIRQDHACRHCRAPIAILDPAAVEKTLDRLDQAE
                     VQRSRRDVEMLGDAIVMREREKSRFEREQRERFNNLDIGDLVVAGVDLLWRVLHR"
     gene            complement(665..1141)
                     /locus_tag="PLN02_09730"
     CDS             complement(665..1141)
                     /locus_tag="PLN02_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI52644.1"
                     /translation="MSSQDENETQGVVFGVLAGVIFGVIALVAGLGVWKASQSAPAAM
                     VAAESDIAPVGEALAKVYFAVGSAALGDGDLMVVARTVDALAAKSDGIVLLSGFHDAS
                     GDPQQNAELARQRAQAVRDALLAGGVAAEKVRMRRPESTLGSGSAEEGRRVEIRVQ"
     gene            complement(1188..1739)
                     /locus_tag="PLN02_09735"
     CDS             complement(1188..1739)
                     /locus_tag="PLN02_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HOI52645.1"
                     /translation="MALKSTVFKAELQVADLDRGHFADYALTVARHPSENDERMMVRI
                     LAFALHAGPDLAFGKGLSSDDECALQEVDPSGVLQTWIEVGQPDETRIRKGCHRARRA
                     VVLTYGARSVDVWWQQIAPQLARFDNLTVLRLSAEETQALGALAERSMRLSCTVQEGT
                     VWLSAGEHSLQLHPDTLLSPNAG"
     gene            1854..2087
                     /locus_tag="PLN02_09740"
     CDS             1854..2087
                     /locus_tag="PLN02_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52646.1"
                     /translation="MSGTLLQPVARKRCASCQCWSGPRAVGTTPGSVAIAAETDIGLC
                     DGGGWHGSERRARSACGHWRIWPVLLDGGTPPA"
     gene            complement(2088..>2387)
                     /locus_tag="PLN02_09745"
     CDS             complement(2088..>2387)
                     /locus_tag="PLN02_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52647.1"
                     /translation="IDHCAIGRLLAMAWRLPASVVAAIEHHHDVQPPADKLVAVTHVA
                     EVVVNALDLAAHQKSRVTHLAASACELIGLNWSDDLGYLFGRIEARSHYLTRIFF"
BASE COUNT          423 a          834 c          759 g          371 t
ORIGIN      
        1 gcacggtgag gtcgttctcg acctctgctt ccactgccac ggcgtctggt tcgacgaatt
       61 cgaaagcgtg cagatcacgc cgggcggcgt catcgaactg ttccggctga tccacgaata
      121 ccgcgaagcg ccccgcctgc cgctgcgcga cgtgctacga tgcccgcgct gcaacgagcg
      181 cctgctgcaa ggtctcgacg tttccaagca cggcggccac ttcaactacc accgctgcct
      241 gcagaagcat ggacggttca cgacctttgc ccagttgatg atcgaaaagg gtttcgtccg
      301 ccagttgtcg ccggccgaaa tcgacgagct ggcgagcaag gtcagcatcg tccgctgcac
      361 cggttgcggc gcgccgatcg acatccgcca ggaccacgcc tgccgccact gccgggcgcc
      421 gatcgccatc ctcgacccgg ccgccgtcga aaagaccctg gaccggctcg accaggccga
      481 ggtccagcgc agccggcgcg acgtcgaaat gctcggcgac gccatcgtca tgcgcgaacg
      541 ggaaaaatcc cgtttcgaac gcgaacagcg cgaacgcttc aacaacctcg acatcggcga
      601 tctggtcgtc gccggcgtcg atctgttgtg gcgcgtcctg caccgctaag ccggcctccc
      661 cgaactactg gacgcgaatc tcgacccgcc gaccttcctc ggcgctgccg ctgcccagcg
      721 tcgattccgg gcgccgcatc ctgacctttt ctgcggccac gccgccagcg agcagcgcat
      781 cgcgcaccgc ctgcgcccgc tgccgagcca gttcggcgtt ctgttgcgga tcgccggaag
      841 cgtcatggaa accggaaagc aggacgatgc cgtcactctt cgcggccagg gcatcgaccg
      901 tccgcgcgac gaccatcagg tcgccgtcgc cgagcgccgc cgaaccgacc gcgaaataga
      961 ccttggccag cgcctcgccg accggcgcga tatcgctttc cgccgccacc atcgctgccg
     1021 gcgccgactg gctcgccttc cagacgccga gaccggcgac cagcgcgatg acgccgaaga
     1081 tgacgccggc gaggacgccg aagaccacgc cctgggtttc gttttcgtct tgactggaca
     1141 tgctgggtaa cctcatttca tggtgaagga aaaatcaccg tggcattcta accagcattc
     1201 ggactcaaca gtgtgtcggg gtgcagttgc agcgagtgct cgccggccga caaccacacc
     1261 gtgccttcct ggacggtgca ggaaaggcgc atcgaacgct cggccagcgc ccccagcgcc
     1321 tgcgtctcct cggccgacag gcgcagtacc gtcagattgt cgaaacgcgc gagttgcggt
     1381 gcaatctgct gccaccagac atcgaccgaa cgggcgccgt aggtcagcac caccgcccgc
     1441 cgggcgcggt gacagccctt gcggatgcgt gtctcatcgg gctggccgac ctcgatccag
     1501 gtctgcagca cgccggaagg atcgacctcc tggagcgcgc attcgtcatc gctcgacagc
     1561 ccctttccga acgccaggtc cggtccggca tggagcgcaa aagcgaggat gcggaccatc
     1621 atccgctcgt cgttttccga aggatgacgg gcgacggtca gcgcgtaatc ggcgaagtgg
     1681 ccgcgatcga gatcggccac ctgcagttcg gccttgaaga cggtggattt gagcgccatt
     1741 gcagcccccg ggaaaagtgc gcgattatcc catggcggca ggcccgccaa ccatccggag
     1801 gagaccatgg aacacgcccc cgtctgcaga cagtcagcaa cagcggctcg gcgatgagcg
     1861 ggacgctgtt gcagcccgtc gcccgcaagc gctgcgccag ttgccagtgc tggagcggcc
     1921 cgcgcgccgt cggcacgacc ccgggcagcg tcgccatcgc cgccgaaacc gacatcggtc
     1981 tgtgcgacgg cggcggctgg cacggcagcg agcggcgcgc ccgctcggcc tgcggtcact
     2041 ggcggatctg gccggtgctg ctcgatggcg gaacgccgcc cgcgtgatca gaagaagatg
     2101 cgggtcagat agtggctgcg tgcctcgatg cggccgaaca ggtaaccgag gtcatcgctc
     2161 cagttcaggc cgatcaactc gcaggcgctc gcggcgaggt gggtaacccg cgatttctgg
     2221 tgcgcggcca ggtcgagggc attgaccacg acttcggcga cgtgggtcac cgcgaccagc
     2281 ttgtcggccg gcggctggac gtcatggtgg tgctcgatcg ctgccaccac cgacgccggc
     2341 agccgccagg ccatcgccag caagcggccg atagcacaat gatcgat
//
LOCUS       DAOSDX010000437         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15506_length_2387_cov_2.920240, whole genome shotgun sequence.
ACCESSION   DAOSDX010000437 DAOSDX010000000
VERSION     DAOSDX010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15506_length_2387_cov_2.920240"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLN02_09750"
     CDS             <1..316
                     /locus_tag="PLN02_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOI52648.1"
                     /translation="VARWFNPSASAIKSGQLVPENISFEHALQLLLENPLLIRRPLME
                     VGDERMVGFDAEAVNAWVGLNGVELPAGNIEACVHGPEGHGSCGGHDHEDEAEHAACG
                     TH"
     gene            294..1565
                     /locus_tag="PLN02_09755"
     CDS             294..1565
                     /locus_tag="PLN02_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOI52649.1"
                     /translation="MPLAAHTDSAVEIAPGVHWVGALDPHLRSFDIILKTANGTSYNA
                     YVVRGETGVAVIDTVKESFSDDFFRRLESVADYAEITTIVLNHLEPDHSGALPELLRR
                     APQARVYLSSRAQMMLKALLKPAGGKPPEYIPVTTDDSVDLGGRTLRFLHTPYLHWPD
                     TQCTYLVEDGILFSGDIFGCHFCDPRLFSDRVGDFRFSFEYYYAHIMRPFREHVLDAV
                     ALIEPLALSTIAPAHGPILRQQPRDYVHRYRELATPRLFNEARVEKTLLVFYISAYGN
                     TRRMAEALAAGAEALGGVRVSLYDLEGGEAGVFTDLIEEADALAFGSPTINGDAVKPV
                     WDVLSSLTTVNVKGKLGAAFGSYGWSGEAVRLIEDRLRGLKLRVPVEGLRIKLVPDNG
                     ELAACRDLGEQLARHLTGRLETRVIDMAELA"
     gene            1582..1869
                     /locus_tag="PLN02_09760"
     CDS             1582..1869
                     /locus_tag="PLN02_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOI52650.1"
                     /translation="MPKAKVTFEDIGVAVTVPAGTRLIEVSEKVGAGITYGCREGECC
                     TCLTKVISGGENLAPPSVLEDQVLKDNMAPRGHRLACQAQILGGEIVVKPA"
     gene            1954..>2387
                     /locus_tag="PLN02_09765"
     CDS             1954..>2387
                     /locus_tag="PLN02_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOI52651.1"
                     /translation="MALITFSSPLHKDKTVYAVAGSRTQTVLSLAKEHHIPIDFGCEN
                     GDCGTCLVKVSSVDAKRSPMGAPLTDHEVRVLRELGKITRVNWIITTWFRSDAYYASG
                     GWRATWKGEGGGVLLNQCPHQLDLMQWLFGMPSRVRAFCHIG"
BASE COUNT          439 a          841 c          733 g          374 t
ORIGIN      
        1 ggtcgcccgc tggttcaacc cgagcgcgtc ggcgatcaag agcggccagc tggtgccgga
       61 gaacatcagc ttcgagcacg cgctgcaact gctgctcgag aatcccctgc tgatccgccg
      121 cccgctgatg gaagtcggcg atgagcgcat ggtcggtttc gatgccgagg cggtcaatgc
      181 ctgggtcggc ttgaacggtg tcgaactgcc ggccggcaac atcgaagcct gcgtccatgg
      241 cccggaaggc cacggcagct gcggcggcca cgatcacgaa gacgaggcag agcatgccgc
      301 ttgcggcaca cactgattcc gccgtcgaga tcgccccggg cgtccactgg gtgggtgccc
      361 tcgatccgca tctgcgcagt ttcgacatca tcctgaagac ggccaacggg acgtcctaca
      421 acgcctacgt ggtgcgcggc gaaaccggcg tggcggtgat cgacacggtc aaggagagtt
      481 tctccgacga tttcttccgc cgtctggagt cggtcgccga ttacgccgaa atcaccacca
      541 tcgtcctcaa ccatctggaa ccggaccata gcggcgccct gcccgaactg ctgcgccgcg
      601 cgccgcaggc gcgggtctat ctgtcgagcc gggcgcagat gatgctcaag gcgctcttga
      661 aaccggccgg cggcaagccg cccgagtaca tcccggtgac taccgacgac agcgtcgatc
      721 tcggcggccg cacgctgcgc ttcctgcaca cgccctacct gcactggccg gacacgcagt
      781 gcacctacct ggtcgaggac ggcatcctgt tctctggcga catcttcggt tgccacttct
      841 gcgatccgcg cctgttcagc gaccgcgtcg gcgatttccg cttttccttc gagtactact
      901 acgcccacat catgcgcccc ttccgcgagc acgtgctcga cgcggtggcg ctgatcgaac
      961 cgctcgcact atcgacgatc gccccggcgc acgggccgat cctgcgccag cagccgcgcg
     1021 actacgtgca ccgctaccgc gaactggcga cgccgcgcct gttcaacgaa gcgcgggtgg
     1081 aaaagaccct gctcgtcttc tacatctcgg cctacggcaa cacccgccgc atggccgagg
     1141 cgctcgccgc cggcgccgag gccctgggcg gcgtgcgcgt ctcgctctat gacctcgaag
     1201 gcggcgaggc cggcgtgttc accgacctga tcgaggaagc cgacgcgctc gccttcggca
     1261 gcccgacgat caacggcgac gcggtgaagc cggtgtggga cgtgctgtcg tcgctgacca
     1321 cggtcaacgt caagggcaag ctcggtgccg ccttcggttc ctacggctgg agcggcgagg
     1381 cggtgcggct gatcgaggac cgcctgcgcg gactcaagct gcgcgtgccg gtggaggggc
     1441 tgcgcatcaa gctcgtcccc gacaacggcg agctggcggc ctgccgcgac ctcggcgaac
     1501 aactggcgcg ccatctgacc ggccgtctcg aaacccgcgt catcgacatg gccgaactgg
     1561 cctgaacctg gagagaacaa aatgcccaaa gcgaaagtca ccttcgaaga catcggcgtc
     1621 gccgtcaccg tcccggccgg cacccgactc atcgaagtct cggaaaaggt cggcgccggc
     1681 atcacttacg gctgccgcga aggcgaatgc tgcacctgcc tgaccaaggt catctcgggc
     1741 ggcgaaaacc tcgccccgcc gagcgtcctc gaagaccagg tgctgaagga caacatggcc
     1801 ccgcgcggcc accggctggc ctgccaggcg cagatcctgg gcggcgagat cgtcgtcaag
     1861 cccgcctgaa tcaccggaca agattaaaag caaagcttca ccccaactac aacagagaaa
     1921 ccccactaac ccgctcaacc cgaaggaaac aacatggcgc tcatcacttt cagcagtccg
     1981 ctgcacaagg acaaaaccgt ttatgccgtc gccggcagcc gtacccagac cgtgctctcc
     2041 ctggccaagg aacaccacat cccgatcgat ttcggctgcg agaacggcga ctgcggcacc
     2101 tgcctggtca aggtctcgtc ggtcgatgcc aagcgtagcc cgatgggcgc tccgctgacc
     2161 gaccacgagg tgcgcgtgct gcgcgaactg ggcaagatca cccgcgtcaa ctggatcatc
     2221 accacctggt tccgctccga cgcctactac gccagcggcg ggtggcgcgc cacgtggaaa
     2281 ggcgagggcg gaggcgtgct gctcaaccag tgcccccacc agctcgactt gatgcagtgg
     2341 ctcttcggca tgccctcccg cgtgcgcgcc ttctgccaca tcggcaa
//
LOCUS       DAOSDX010000438         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15528_length_2384_cov_3.708888, whole genome shotgun sequence.
ACCESSION   DAOSDX010000438 DAOSDX010000000
VERSION     DAOSDX010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15528_length_2384_cov_3.708888"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /gene="metX"
                     /locus_tag="PLN02_09770"
     CDS             complement(<1..439)
                     /gene="metX"
                     /locus_tag="PLN02_09770"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285899.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOI52652.1"
                     /translation="MPGQSVGVVEAQRIHFSEPLHLRSGGVLPSYDLAVETYGTLNAD
                     KSNAILVCHYFSGSAHAAGRYQDSDPLPGWWDGVIGPGKIIDTDRYFVVCSDSLCNVR
                     ALDGHVVTTGPASIDPDTGKAYGLDFPVTTVGDFVHVQRALLDH"
     gene            694..1281
                     /locus_tag="PLN02_09775"
     CDS             694..1281
                     /locus_tag="PLN02_09775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOI52653.1"
                     /translation="MIEQRRHQRIRFGVSPPVRIGFGGKAGEGSVENLSMSGLMLRSP
                     EPLEIGRLVGCEFSLFGSPLIDIAVNIVSRIGDGLYGARFQIGLINQVLIEDAIRDAL
                     ASGKASVLSVHELSGRKVMRIVGGLNGALRNDFMHALIRVGVEELDVGGVTLVEQAGL
                     ALCLVATDRHGVSITSRSDCFADAWAQAVPTSGSH"
     gene            1327..>2384
                     /locus_tag="PLN02_09780"
     CDS             1327..>2384
                     /locus_tag="PLN02_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI52654.1"
                     /translation="MLPFGTLLAEDELFFAELPVVASVSRLPQRLADAPASVTVIDRE
                     MIRASGYRSLNDLFRLVPGFQTFAHSDVGARVNYHGITDDNDFSPRVQVLIDGRSLHS
                     PLFRGGVNWALIPVALEDIERIEVVRGSNSVSYGTNAFLGVVNIVTIDPSLVRGVSVS
                     ASRGTQGVNDYSVRGGGKLGETGNFRLSYQQTSDDGLKGNYDWEDSYRNRRFDARFDY
                     AVNLRDSLEFSLGKVEGRFLSGSLDKVTRLSDPCDDPLRNRDESSTWMQARWLRALSE
                     TADFSLRYTFSEDRGDNAYRIDPVTCGVVPYPKVNESGDWGRRHEIEATHNFVPMAKT
                     RLVWGASWRHDEMSSQTM"
BASE COUNT          397 a          691 c          801 g          495 t
ORIGIN      
        1 ggtggtcgag gagggcgcgc tggacgtgca cgaagtcgcc cacggtggtg acggggaagt
       61 cgagcccata ggccttgccg gtgtcggggt cgatgctcgc cgggccggtg gtcaccacgt
      121 gaccgtcgag ggcgcgcacg ttgcagaggc tgtcgctgca gaccacgaag tagcggtcgg
      181 tgtcgatgat cttgccgggc ccgatcacgc cgtcccacca gccgggcagg gggtcgctgt
      241 cctggtagcg cccggcggcg tgggcgctgc ccgagaagta gtggcagacc aggatggcgt
      301 tggatttgtc agcgttcagc gtgccgtagg tctcgacggc caggtcgtag gacggcagga
      361 cgccgccgct gcgcaggtgt agcggttcgc tgaaatggat gcgttgcgcc tcgacgacgc
      421 cgacggactg tcccggcatg atgctcccga aaacaaaaaa cccagttggc cgaaacgcga
      481 actgggcggt tcccgtttta gctgcatttt tgagcgcccg caatcagagt acaaatcggc
      541 gctgccggat gatttcggca gaaggaagat aaccagccct ccggcaaatg tcaattcgag
      601 gcaattgccg ggttcaggcg tcgtgacggc ttatccggtc gcgtactttt ggtaccattg
      661 tcacccgggg attgatgaag attggggaga agcatgatcg agcagcggcg acatcagcgc
      721 atccgtttcg gggtttcgcc cccggtcagg atcggctttg ggggtaaggc tggcgagggt
      781 tccgtcgaga acctgtcgat gtccggcttg atgttgcgca gcccggagcc gctcgagatc
      841 ggccgtctgg tcggctgcga gttttcgctg ttcggttcgc cgttgatcga cattgcggtc
      901 aatattgtca gccggatcgg cgatgggctg tatggtgccc gcttccagat cggcttgatc
      961 aatcaggtgc tgatcgagga tgcgatccgc gatgccctgg cttccggaaa ggcttcagtg
     1021 ctttctgtcc acgaactgag tgggcgcaag gtgatgcgta tcgtcggcgg cctcaatggt
     1081 gccctgcgca atgacttcat gcatgccttg atccgggtcg gcgttgagga gctcgatgtc
     1141 gggggggtga cgctggtcga gcaggcagga ctggcgctct gtctggtggc gaccgaccgg
     1201 cacggggtaa gcatcaccag tcgttccgac tgctttgccg acgcctgggc gcaggccgtg
     1261 ccgacgtcgg gaagccactg atccgtgcac atgaagcggc ggcgctatct ttccgtgctg
     1321 gtttgcatgc tgccgtttgg cacgctgctg gccgaggacg agttgttttt cgccgagttg
     1381 ccggtggtcg cctcggtcag ccgcctgccg cagcggctcg ccgatgcgcc ggcttcggtg
     1441 acggtgatcg accgggagat gatccgtgct tccggctatc gctcgctcaa cgatctgttc
     1501 cgtctggtgc cgggtttcca gacctttgcc cacagcgacg tcggcgcccg ggtcaattac
     1561 cacggcatca ccgacgacaa cgatttttca ccacgggtcc aggtgctgat cgacggtcgc
     1621 tcgctgcatt cgccgttgtt ccggggcggg gtcaattggg cactgattcc cgtcgccctc
     1681 gaggatatcg agcgcattga ggtggtgcgc ggcagcaaca gcgtttccta cgggaccaat
     1741 gccttcctcg gtgtcgtcaa tatcgtcacc atcgatccct cgttggtacg cggtgtttcg
     1801 gtttccgcca gccgtggcac gcagggggtc aacgattact cggtgcgtgg cggtggcaag
     1861 ctgggtgaga ccgggaattt ccgcctgagc taccagcaga ccagcgacga tggcctgaaa
     1921 ggcaattacg actgggagga tagctaccgc aatcgtcgct tcgacgcccg tttcgactac
     1981 gcggtgaatc tccgggacag cctggagttc tcgctgggca aggtcgaggg acgcttcctg
     2041 tctggctcgc tcgacaaggt cacccgcctt tcggatccct gcgacgatcc cctgcgcaac
     2101 cgcgacgaat cctccacctg gatgcaggcg cgctggctgc gggcgctttc cgaaactgcc
     2161 gatttctcgc tgcgctacac cttcagcgaa gatcgcggcg acaatgccta tcgtatcgat
     2221 ccggtcactt gcggcgtggt tccctaccct aaggtgaacg aatccggtga ctgggggcgg
     2281 cggcacgaaa tcgaggccac ccacaatttc gtgccaatgg caaagacgcg tctggtctgg
     2341 ggggcgagtt ggcgacatga cgaaatgtcc tcgcagacca tgat
//
LOCUS       DAOSDX010000439         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15538_length_2383_cov_3.436856, whole genome shotgun sequence.
ACCESSION   DAOSDX010000439 DAOSDX010000000
VERSION     DAOSDX010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15538_length_2383_cov_3.436856"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLN02_09785"
     CDS             complement(<1..645)
                     /locus_tag="PLN02_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52655.1"
                     /translation="MPSSRLHRFGLASRLVALAAIAALLVSTVSGWILRQSVQGTLLR
                     HFEQRLAERTERLLAGFSVLPGSSQIHWLGRSNDEFGRIFSGWYWQLEGSGELQASRS
                     LWDSRLDTASNRPVPGNPELRQGVDPQGLAVMGIVRVIEVDGQAAHLHVYGPAAEIAS
                     ELDRLDRILLLTQLGLIVSLLLTSILQVRLGLLPLRRLRDRLAAVRTGSAASVGN"
     gene            complement(635..1303)
                     /locus_tag="PLN02_09790"
     CDS             complement(635..1303)
                     /locus_tag="PLN02_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI52656.1"
                     /translation="MKVLLVEDDRELAAKIAAALSADGMIVDTVHDGDEAAFVGSTED
                     YDAAVLDLGLPGQDGVSVLRHWREQGRRLPVLVLTARGRWSDKLASFDAGADDFLTKP
                     FLHEEVALRLRVLKRRALGHSSPILQAGPLSYDANADRFTLDGAPLNLTAQEKRILAY
                     LIQRKDCVVSRNEISEHVYARDLDPDSNTLDVLIGRIRRKLGVPLIQTERGRGFCLTE
                     VDAV"
     gene            complement(1300..2004)
                     /locus_tag="PLN02_09795"
     CDS             complement(1300..2004)
                     /locus_tag="PLN02_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOI52657.1"
                     /translation="MKLLPALLIPLLLLPPAHAEEQYVIRRAVEAGQLKPLAEILAGV
                     QKTHPGKVLDVDLERDLNGRRIYEVTVLKSDGQRSKLLVDAVSGGELPHATPAMPRLD
                     MAKALRALLARHPGKVLELELKLAADSRLIYEVQVILQDGQLREFVIDARSGELIDGN
                     GHRQEVLKKLMPLPDLLARLPARYRGTIHEVELEYDQDGHYFYEVEVRLNDGRVFELD
                     IDAVSGKLLNDEEVSR"
     gene            2116..>2383
                     /locus_tag="PLN02_09800"
     CDS             2116..>2383
                     /locus_tag="PLN02_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HOI52658.1"
                     /translation="MSTTRSLSVPLSLSAPRLRWPHGGFLLAILFVLAGAALFGRWPL
                     VALLWQQATADAAQRERAVWLPDYRVDIEARPLAGIAANLSGLTY"
BASE COUNT          356 a          753 c          847 g          427 t
ORIGIN      
        1 gttgccgacg ctggcggcgc tgccggtgcg caccgccgcc agccggtcgc gtagccggcg
       61 cagcggcagc agtccgagac ggacctgcag gatgctggtg agcagcagcg agacgatcag
      121 gccgagttgg gtgaggagca ggatgcggtc gaggcgatcg agttcgctgg cgatctcggc
      181 ggccgggcca tagacgtgca ggtgggccgc ttgtccatcc acttcgatga cgcggacgat
      241 gcccatgacg gccagtcctt gcgggtcgac cccctgccgc agctcggggt tgccgggtac
      301 gggccggttg ctagcggtat ccaggcggct gtcccacagc gagcgcgaag cctgcagttc
      361 gccgctgcct tccaactgcc agtaccagcc ggagaagatg cggccgaatt cgtcgttgct
      421 gcgcccgagc cagtggatct ggctgctgcc gggcaggacg gagaaaccgg cgagcagtcg
      481 ctcggtccgt tctgcgaggc gttgctcgaa atgccggagc agggtgccct gcacgctctg
      541 ccgcaagatc cagccactga ccgtgctgac cagcagggcg gcgatcgcgg ccagcgcgac
      601 caagcgcgag gccagaccaa aacggtgcag ccggctagac ggcatcgact tcggtaaggc
      661 agaagccgcg cccgcgttcg gtctggatca gcggcacgcc gagcttgcgc cggatacggc
      721 cgatcaggac atcgagggtg ttggaatccg ggtcgaggtc gcgcgcgtag acgtgttcgc
      781 tgatttcgtt gcggctgacg acgcaatcct tgcgctggat caggtaggca aggatgcgtt
      841 tttcctgggc ggtcaggttg agcggcgcgc cgtcgagcgt gaaacggtcg gcgttggcgt
      901 cgtaactgag tgggccggcc tgcaggatcg gcgaactgtg accgagggcg cgccgcttca
      961 ggacacgcag gcggagcgcg acttcctcgt gcaggaaagg cttggtcagg aagtcgtcgg
     1021 cgccggcgtc gaaactggcc agcttgtcgc tccagcggcc gcgcgcggtc agtaccagca
     1081 ccggcaggcg gcgcccctgc tcgcgccagt ggcgcaacac cgagacgccg tcctggccgg
     1141 gcaggccgag atcgagcacg gcggcatcgt agtcctcggt gctgccgacg aaagccgcct
     1201 cgtcgccgtc gtgcacggta tcgacgatca tgccgtcggc gctcagcgcg gcggcgattt
     1261 tcgcggcgag ttcgcggtcg tcctcgacca gcagcacctt catcggctga cttcctcgtc
     1321 gttgagcagc ttgccgctga ccgcgtcgat gtcgagttcg aagacgcggc cgtcgttgag
     1381 tcggacttcg acctcgtaga aatagtggcc gtcctgatcg tattccagtt cgacctcgtg
     1441 gatggtgccg cggtagcgcg cgggtaggcg ggcgagcagg tcgggcagcg gcatcagctt
     1501 cttcaggacc tcctggcggt ggccgttgcc gtcgatcagc tcgccactcc gggcatcgat
     1561 gacgaactcg cgcaactgtc cgtcctgcag gatgacctgg acctcgtaga tcagccggct
     1621 gtcggccgcc agcttcagtt ccagctcgag gaccttgccc gggtggcggg cgagcagcgc
     1681 gcgcagcgcc ttggccatgt cgagacgcgg cattgccggt gtcgcgtgcg gcaactcccc
     1741 gccgctcacc gcatcgacca gcagcttgct gcgttgaccg tcgctcttga gcacggtgac
     1801 ctcgtagata cgcctgccgt tgaggtcgcg ttcgagatcg acgtcgagta ccttgcctgg
     1861 atgcgttttc tggacaccgg ccaggatttc cgccagcggt ttgagctggc cggcctcgac
     1921 ggcgcggcgg atgacgtatt gctcttcggc gtgggcgggc ggcagcagga gtagggggat
     1981 caggagggcg ggcaggagtt tcatggggcg catgctgcgg caaccaggat aaacggttga
     2041 tgaattgtag cgtcaggcgg cgtttagcgt cggggcggca ggatggtgcc gtcttcgact
     2101 gcaactgttt aggtcatgtc caccactcgc agcctctctg ttccgctttc tctatccgct
     2161 ccccggctac gctggccgca cggcggcttc ctgctggcga tcctgttcgt tctcgccggt
     2221 gccgcgctgt tcgggcgctg gccgctcgtc gccctgctct ggcagcaggc gacggccgat
     2281 gctgcgcaac gcgagcgggc ggtgtggctg cccgactacc gggtcgacat cgaagcccgg
     2341 ccgttggccg gcatcgccgc caatctctcc ggactgactt acg
//
LOCUS       DAOSDX010000440         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15543_length_2383_cov_2.937715, whole genome shotgun sequence.
ACCESSION   DAOSDX010000440 DAOSDX010000000
VERSION     DAOSDX010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15543_length_2383_cov_2.937715"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLN02_09805"
     CDS             complement(<1..873)
                     /locus_tag="PLN02_09805"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HOI52659.1"
                     /translation="MDSFKALLIEESEGKVANRFVDMTVADLDAGEVLIRVAYSSVNF
                     KDALAATGAGKIIRRFPCVGGIDLSGTVVASDDARFKPGDPVIATSFDIGVAHHGGYA
                     EMARVPADWVVPLPAGMSLYEAMVLGTAGFTAALGVVRMEENGLTPAKGPVIVTGATG
                     GVGSLAIDMLAQRGYHVVALTGKESEADYLRSLGAAEVMLRQSLDLSKIRPLDRARWA
                     GAVDNLGGDVLAWIASTMEQGGTIASIGLAASMKLNTSVSPFILRGDSLLGIDSGYIR
                     EPYLHGVWQRLASDL"
     gene            1110..1517
                     /locus_tag="PLN02_09810"
     CDS             1110..1517
                     /locus_tag="PLN02_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOI52660.1"
                     /translation="MSFVERYTNVTQDVILEGLKLVGVRYKMGGNSVENGLDCSSFVQ
                     MVFKESIGAYLPRTAREQSQVGEQIDASQLQPGDLVFFNTMRRTFSHVGIYLGDNHFM
                     HAPRAGSEVRVENMNNSYWQSRFNGARRIIEAR"
     gene            1629..>2383
                     /locus_tag="PLN02_09815"
     CDS             1629..>2383
                     /locus_tag="PLN02_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOI52661.1"
                     /translation="MKLKVSLAALSIAAALQPALAEEKVLNLYSARHYQTDEALYADF
                     TKQTGIKINRIEGKEDELLERIRNEGANSPADVLLTVDASRLAKAREMGLFAPLSSPI
                     LEKRIPAHLRTAEWAAFSTRARVIVYNKAKVSAADVQNYSDLANPKLKGKFCSRSGSH
                     PYNLSLLASVIAHDGEARAEELARGMVANFARAPKGGDTDQIKAVAAGECCVAISNSY
                     SVARLMRSNKAAAQKVTEQVGIAWPNQETTGTH"
BASE COUNT          511 a          786 c          651 g          435 t
ORIGIN      
        1 cagatcgctg gccaaacgct gccagacgcc atgcagatag ggctcgcgaa tgtagcccga
       61 atcaatgccg agcagcgaat ccccgcgcag gatgaatggc gaaacagagg tattcagctt
      121 catgctggca gccaggccga tgctggcgat ggtgccgccc tgctccatcg tgctggcgat
      181 ccaggccagc acgtcgccgc ccagattgtc cacggcgccc gcccagcgcg cccgatcgag
      241 cggccggatc ttgctcagat cgaggctctg gcgcagcatc acttccgccg cgccgaggct
      301 gcgcaggtag tctgcttcgc tttccttgcc ggtcagcgcc acaacgtgat agccgcgctg
      361 cgccagcatg tcgatggcca gactgccgac tccgccggtc gcgccggtaa cgatcaccgg
      421 tcccttggcc ggcgtcagac cgttttcttc catccggacc acgcccagcg ccgcagtgaa
      481 acccgccgtg cccagcacca tcgcctcgta aagcgacatt ccggcaggca gcggcaccac
      541 ccaatcggcc ggcaccctgg ccatttcggc gtaaccgccg tgatgtgcaa cgccgatgtc
      601 gaaactggtc gcaatgaccg ggtctccggg cttgaatctg gcatcatcgc tggcgacgac
      661 agttccggaa agatcaatgc caccgacaca gggaaagcgc cggatgatct tgccggcacc
      721 ggtagcggcc agcgcatcct tgaaattgac gcttgaatag gcgacccgga tcaggacttc
      781 gccagcatcg aggtcagcaa ccgtcatatc gacgaagcga ttggcgacct tgccttcgct
      841 ctcttcaatc aacaatgcct tgaacgaatc cacggtgacc tgcctcctca cgcctagggg
      901 ttgggattgt attcataatt acggattatg aacatagttt catttccact cgccagcagc
      961 ttcgatgggc tttacagagc ggcagatttc aaatagatta cgcgcccatg tatcgacaaa
     1021 tcacatccct tctgcttggc tccgcacttc tcctgaccgg cgtctgcccg gttggcgcgg
     1081 ccgaagcgac gcgttcgccg gaaactccga tgtccttcgt cgaacgttac accaacgtca
     1141 ctcaggatgt catcctggaa ggcctcaagc tggttggcgt gcgctacaag atgggcggca
     1201 acagcgtcga gaatggcctg gactgcagca gtttcgtcca gatggtgttc aaggaaagca
     1261 tcggtgccta cctgccgcgc accgcgcgcg aacaaagcca ggttggcgaa caaatcgacg
     1321 ccagccagtt gcagcccggt gatctcgtct tcttcaacac catgcgccgc accttctccc
     1381 acgtcggcat ctacctcggc gacaatcact tcatgcatgc cccgcgcgcc ggctcggaag
     1441 tccgggtcga gaacatgaac aacagctact ggcaaagtcg cttcaacggc gccagaagga
     1501 tcatcgaagc ccgctgatcg aaattacggc cgttcggccg tttttttcac ccacaattgg
     1561 taatgtttct cattaataat ttttgccgta gaatagaaaa tgccccatcc gcaaaactgg
     1621 agaaaccaat gaagttgaaa gtctcgctcg ccgccttgtc cattgccgcc gccctgcaac
     1681 cggcgctggc cgaggaaaaa gtgctcaatc tgtactcggc ccgtcactac cagaccgacg
     1741 aagcgctgta cgccgatttc accaagcaga ctggcatcaa gatcaaccgg atcgaaggca
     1801 aggaggacga gctgctcgaa cgcatacgca acgaaggcgc caacagccca gccgacgtcc
     1861 tgctgaccgt cgacgcctca cgcttggcca aggcgcggga aatgggactg ttcgcgccgc
     1921 ttagctcgcc gattctggag aaacgcatcc cggcccacct ccgcaccgcc gaatgggcag
     1981 ccttttccac ccgtgcccgc gtcatcgtct ataacaaggc gaaagtctcc gcggccgatg
     2041 tgcagaacta cagcgacctc gccaacccga aactgaaggg aaaattctgc tcgcgttctg
     2101 gctcgcaccc ctacaacctg tctctgctcg cctcggtgat cgcgcatgac ggcgaggcca
     2161 gggccgaaga actggcgcgt ggcatggtcg ccaacttcgc ccgcgctccg aaaggcggtg
     2221 atacggacca gatcaaggcg gtcgccgccg gcgaatgctg cgtcgccatt tccaacagct
     2281 actccgtcgc ccgcctgatg cgttcgaaca aggccgctgc ccagaaagtc acggaacagg
     2341 tcgggatcgc ctggcccaat caggagacga ccggcaccca cat
//
LOCUS       DAOSDX010000441         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15563_length_2381_cov_2.986242, whole genome shotgun sequence.
ACCESSION   DAOSDX010000441 DAOSDX010000000
VERSION     DAOSDX010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15563_length_2381_cov_2.986242"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLN02_09820"
     CDS             <1..950
                     /locus_tag="PLN02_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOI52662.1"
                     /translation="LIVGAMRRLPAGRARKPGSPEERLRTLAGMANELCEAIALATPE
                     NRDQEFRKIVTRFNEAIPLDDAQVMQTLRRAVEEVGELARIVHLGVQNTTFGRQMRHF
                     AKLAQSPGEATGTGQTEDSLGSIALGDDVVPGTVGDAASAVGVDAQAVLTAGIQDISN
                     TLVDGFKLNDILRIILETMYRAMGFKRVILCVRDPRSNTMQGRFGFGPEASELARGFR
                     FPLSFTPDIFHAATSKGVDILIRDIDDPKIAQRVPAWYRSLLPARSFVVFPLNLKGNP
                     VALIYADCDEADGIHIPEKELSLLRTLRNQAVLAIKQGG"
     gene            complement(998..1825)
                     /locus_tag="PLN02_09825"
     CDS             complement(998..1825)
                     /locus_tag="PLN02_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOI52663.1"
                     /translation="MMRSLPAFPTFAASLRWLLALLAAAVLAGCSSLPAEQDETAGWS
                     ANRLYTAAREAQTEGSWEIAVKMLEKLEARYPYGRYAQQAQLELAYVYWKSNEPGSAL
                     AAADRFIKMHPNHPAVDYAMYLKGLVNFNEDLGLIGHISNQDLTERDPKAAREAFESF
                     KELVTRFPDSKYAPDSIKRMNYLVNALASLEVHVARYYIKRGAYIAAANRAQVAVETY
                     PRAPAIEEALYILVLAYEKLEMKELRDDAERVLKQNFPNSRFYTEGIERKTAWWQLW"
     gene            1824..>2381
                     /locus_tag="PLN02_09830"
     CDS             1824..>2381
                     /locus_tag="PLN02_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOI52664.1"
                     /translation="MNDPLENSGDYSPSEPLKMTVPDVMGGRRLDQALAEMLPQYSRN
                     RLQSWIREGCVTLYGEPVGEPKRKLVGGEQLLLELPDDEFLGAEQAEDIPLAIVYEDE
                     ALLVINKPAGLVVHPGSGNRNGTLMNALLHHVPGIGEVPRAGIVHRLDKETSGLLVVA
                     KTLEAQTDLVRQLQARTVKRQYLAVA"
BASE COUNT          414 a          737 c          807 g          423 t
ORIGIN      
        1 cgctgatcgt cggcgccatg cggcgcctgc cggcgggccg ggcgcgcaag ccgggctcgc
       61 cggaagagcg gctgcggacg ctggccggca tggccaacga gttgtgcgag gcgattgccc
      121 tggcgacgcc ggagaaccgc gaccaggagt tccgcaagat cgtcacccgt ttcaacgagg
      181 cgattccgct cgacgacgct caggtgatgc agacgctgcg gcgggcggtc gaggaggtcg
      241 gcgaacttgc ccggatcgtc catctggggg tgcagaacac gacgttcggc cggcagatgc
      301 ggcatttcgc caagctcgcg cagagcccgg gcgaagcgac gggcaccggc cagacggagg
      361 attcgctcgg cagcattgcc ctcggcgacg atgtcgtgcc gggtaccgtc ggtgacgctg
      421 ccagcgccgt cggcgtcgat gcgcaggcgg tgctgaccgc cggcatccag gacatcagca
      481 acacgctggt cgacggtttc aagctcaatg acatcctgcg catcatcctg gaaaccatgt
      541 accgggcgat gggtttcaag cgcgtcatcc tgtgcgtgcg cgatccgcgc agcaacacca
      601 tgcaggggcg tttcggcttc ggcccggagg ccagcgaact ggcgcgcggt tttcgctttc
      661 cgctcagctt cacgccggac atcttccacg ccgcgacttc gaaaggcgtc gatatcctga
      721 tccgcgacat cgacgatccg aagatcgccc agcgcgttcc cgcgtggtac cgcagcctgc
      781 tgccggcgcg ttccttcgtc gtctttccgc tcaacctgaa gggcaacccg gtggcgctga
      841 tctacgccga ttgcgacgag gcggacggca tccacatccc ggaaaaggaa ttgtcgctgc
      901 tgcgcaccct gcgcaaccag gcggtgctgg cgatcaagca gggcggatag caaaagcccg
      961 ccggaaggcg ggctgtcgag gacgattcag gggcgcttca ccagagttgc caccaggcgg
     1021 tcttgcgttc tatcccctcg gtgtagaaac ggctgttcgg gaaattctgc ttcagcaccc
     1081 gttcggcgtc gtcgcgcaat tccttcattt ccagcttttc gtaagcaagg acgaggatgt
     1141 acagcgcttc ctcgatcgcc ggcgcgcgcg ggtaggtttc gacggcgacc tgcgcccggt
     1201 tggcggcggc aatgtaggcg ccgcgcttga tgtaatagcg ggcgacgtga acttccagcg
     1261 aggccagtgc attgaccagg tagttcatgc gcttgatcga gtccggcgcg tatttgctgt
     1321 ccgggaagcg ggtgaccagt tccttgaacg actcgaaggc ttcgcgcgcc gccttggggt
     1381 cgcgttcggt caggtcctgg ttgctgatgt ggccgatcag gccgagatcc tcgttgaaat
     1441 tgacgaggcc cttcaggtac atcgcgtaat cgacggcggg atggttcggg tgcatcttga
     1501 tgaagcggtc ggcggcggcg agcgccgatc ccggttcgtt cgatttccag tagacgtagg
     1561 ccagttcgag ctgcgcctgc tgggcgtagc ggccgtaggg gtagcgggct tcgagttttt
     1621 ccagcatctt gaccgcgatc tcccacgatc cttcggtctg cgcctcgcgg gcggcggtgt
     1681 agaggcgatt ggcggaccag ccagcggttt cgtcttgttc ggccggcagc gaactgcagc
     1741 cggcaagtac ggcagcggca aggagtgcga gcagccagcg aaggctggcg gcgaaagtcg
     1801 ggaatgcagg taaactgcgc atcatgaatg atcctcttga aaactcgggc gattatagcc
     1861 caagcgagcc gctcaaaatg acggttcccg atgtcatggg cggtcgccgg ctcgaccagg
     1921 cgctcgccga aatgcttccc caatattccc gcaaccgcct gcaatcctgg attcgggagg
     1981 gctgcgtcac gctctacggc gagccggtcg gcgaacccaa gcgcaagctg gtgggcggcg
     2041 aacagttgct gctcgaactg cccgacgacg aattcctcgg tgccgagcag gcggaagaca
     2101 ttccgctcgc catcgtctac gaggacgagg cgctgctggt gatcaacaag ccggccgggc
     2161 tggtcgtcca tccgggcagc ggcaaccgca acggcaccct gatgaacgcg ctgctgcacc
     2221 acgtgccggg catcggcgag gtgccgcggg ccggtatcgt gcatcgcctg gacaaggaaa
     2281 ccagcggcct gctggtcgtc gccaagacat tggaagcgca gaccgacctg gtgcggcagt
     2341 tgcaggcgcg caccgtcaag cgccagtacc tggcggtcgc c
//
LOCUS       DAOSDX010000442         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15564_length_2381_cov_2.904557, whole genome shotgun sequence.
ACCESSION   DAOSDX010000442 DAOSDX010000000
VERSION     DAOSDX010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15564_length_2381_cov_2.904557"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PLN02_09835"
     CDS             complement(<1..542)
                     /locus_tag="PLN02_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOI52665.1"
                     /translation="MSESPQFPIPPAPFKGDSREVDAGACFDWLAQGWAMFLAHPGIW
                     LGASILLLVILMAISIVPLFGQIAAHLLVPLFGAGMLQISRAIADGGEPQIGDLFAGF
                     RSQAGQLVMVGVFFAIGVFGIALIAFLLISGGLLGGAISGRIGGFGIAFGGFMLAGLL
                     VMLLSVPVIMATWFAPALVY"
     gene            complement(552..1478)
                     /locus_tag="PLN02_09840"
     CDS             complement(552..1478)
                     /locus_tag="PLN02_09840"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOI52666.1"
                     /translation="MSVYTTVGRAELAAWLRPLAAGELIDHAGIAAGMQNSNYFVTTS
                     GGRFVLTLFEALDPAALDFYLRLQAHLADAGLPCPRPLADAAGRLWWPLCGKPAALLT
                     CLPGRAVDTPDATQCRAVGAALAQLHLAAADLAGAPANPCGAAWCLALGESLLPLLPA
                     ADGALLADELDFQSRQDRSALPQGVIHADLFRDNVLWRDDGELGGLLDFYFAGVDAWL
                     FDLAVVANDWCGDAGRLAALIDGYTGVRPLTAAERLAWPALRRAAALRFWLLRLDAKL
                     RPRAGEVVTIKNPDDFKALLLRLRLAADALPG"
     gene            complement(1482..1811)
                     /locus_tag="PLN02_09845"
     CDS             complement(1482..1811)
                     /locus_tag="PLN02_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HOI52667.1"
                     /translation="MRRLLPILLFAALPVWAQQGELQPLPAVPPPPPEMEPFDAALEP
                     QVTIVRNDKETREEFRANGKLYMVKVTPTHGKPYYLVDRQGDGVMVESEINPNPVRPP
                     MWVIHSW"
     gene            complement(1843..>2381)
                     /locus_tag="PLN02_09850"
     CDS             complement(1843..>2381)
                     /locus_tag="PLN02_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOI52668.1"
                     /translation="ELTERVARQLSDSGFSVISGGGPGIMEAANKGAFHGKSPSVGLN
                     IQLPHEQQSNPYQDISQTFRHFFARKYMFVRFASAYVVMPGGFGTLDELMEALTLIQT
                     GKARKIPLILVCSDFWKGLIDWFKDTLVREKMIDPEDTELIQIIDDPEKVVEAIFKHY
                     EARPFSPLPNEREMMLNL"
BASE COUNT          455 a          759 c          808 g          359 t
ORIGIN      
        1 aaatagacca gcgccggcgc gaaccaggtg gccatgatca ccggcaccga gagcagcatg
       61 acgagcaggc cggccagcat gaagccgccg aaggcgatgc cgaagccgcc gatccgcccg
      121 gaaatcgcgc cgcccagcaa accgccgctg atcagcagga aggcgatcag cgcgatgccg
      181 aagacgccga tggcgaagaa gacgccaacc atcaccagtt gcccggcctg gctgcggaag
      241 ccggcgaaga ggtcgccaat ctgcggttcg ccgccgtcag cgatggcgcg cgaaatctgc
      301 agcatgccgg caccgaacag cggcaccagc aggtgggcgg cgatctgccc gaacagcggg
      361 acgatggaaa tggccatcag aatgaccagc agcaggatgc tcgcccccag ccagatgccg
      421 ggatgggcga ggaacatcgc ccagccctgg gccaaccagt cgaaacaagc gccggcgtcg
      481 acctcacggc tgtcgccctt gaacggcgcg gggggaatcg ggaattgcgg actttcgctc
      541 atggccggat gctagccggg gagcgcgtcg gcggcaaggc gcaagcgcag cagcagggcc
      601 ttgaaatcgt caggattctt gatcgtcacc acttcgccgg cgcgcgggcg caattttgca
      661 tcaagacgca gcagccagaa acgcagcgcc gccgcccgcc gcaaggccgg ccaggccagc
      721 ctttcggctg ccgtcagcgg gcgcaccccg gtatagccgt cgatcagcgc cgccagccgc
      781 ccggcgtcgc cgcaccagtc gttggcaacc accgccaggt cgaacagcca ggcatcgacg
      841 ccggcaaaat agaaatcgag caggccgcca agttcgccat cgtcgcgcca cagcacgttg
      901 tcgcggaaca ggtcggcgtg aatcacgccc tgcggcagcg ccgagcggtc ctgccgcgac
      961 tggaagtcca gttcgtcggc aagcagcgcg ccatcggcgg ccggcagcag aggcaagagg
     1021 ctttccccca gcgccaggca ccaggcggcg ccacagggat tggccggcgc tccggcaaga
     1081 tcggcggcgg ccagatgcaa ctgcgccagc gcggcgccga cggcgcggca ttgcgtcgca
     1141 tcaggcgtgt cgacggcgcg cccgggcaag caggtgagca gcgccgccgg cttgccacac
     1201 aacggccacc acaggcggcc agcggcgtca gccagcggcc gcggacaggg cagcccggca
     1261 tcggccagat gcgcctgcag gcgcagatag aaatcaagcg ctgccggatc gagcgcttcg
     1321 aacagcgtca gcacgaagcg gccgccgctc gtcgtcacga aataattgga attctgcatg
     1381 ccggcggcga tgccggcgtg gtcaatcagt tcgccggcgg cgagcggccg gagccaggcc
     1441 gcgagctcgg cgcggccgac cgtggtgtag acggacaagg cttaccagct atggatgacc
     1501 cacatcggcg gtcgcaccgg gttgggattg atctcgcttt cgaccatcac gccgtcgccc
     1561 tggcgatcga ccaggtaata gggtttgccg tgcgtcggcg tcaccttgac catgtagagc
     1621 ttgccgttgg cgcggaattc ctcgcgcgtt tccttgtcgt tgcggacgat ggtcacctgc
     1681 ggttcgagtg cggcgtcgaa cggttccatt tccggcggcg gaggcggcac ggcgggcagc
     1741 ggttgcagtt cgccctgttg cgcccagacg ggcaaggcgg cgaagagcag gatggggaga
     1801 agacggcgca tggtttttcc tttcggtatg ccggccattt tattacaggt tgagcatcat
     1861 ctcccgctcg ttcggcaagg ggctgaacgg gcgtgcttcg taatgcttga agatcgcttc
     1921 gacgaccttt tccgggtcgt cgatgatctg gatcagttcg gtgtcttcgg ggtcgatcat
     1981 cttctcgcgc accagagtgt ccttgaacca gtcgatcaaa cccttccaga aatccgagca
     2041 gaccaggatc agcggaatct tgcgcgcctt gccggtctgg atcagggtca gggcctccat
     2101 cagttcgtcg agcgtgccga agccgcctgg catgacgacg taggcgctgg cgaagcggac
     2161 gaacatgtac ttgcgcgcga aaaagtggcg gaaggtctgc gagatgtcct gatagggatt
     2221 gctctgctgc tcgtgcggca actggatgtt gagcccgacc gacggtgact tgccgtggaa
     2281 ggcgcccttg ttggccgctt ccatgatgcc gggaccgcca cctgagatca ccgagaagcc
     2341 ggaatcggaa agctggcggg caacgcgctc ggtcagttcg t
//
LOCUS       DAOSDX010000443         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15591_length_2377_cov_2.730835, whole genome shotgun sequence.
ACCESSION   DAOSDX010000443 DAOSDX010000000
VERSION     DAOSDX010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15591_length_2377_cov_2.730835"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLN02_09855"
     CDS             complement(<1..995)
                     /locus_tag="PLN02_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOI52669.1"
                     /translation="MSKLQTAVLVVTLALAACAVPPRTVSPEGAAKAPDCPPCAEAAA
                     EKPAAPAFSRVFTLAAWSDLPGWQEDDLSAAWQPFLKSCQALRSRAGGAPWQQACAHA
                     AEVDARSGEAIRRYFENVMQPYTVSTDDRSAVEGMITGYYEPLLQGSRQRDKRNLHPV
                     HGVPDNLLTIELGDLFPELKGKRVRGRIEGNKVVPYWSRGEIAAQGKRLPARTLAWVD
                     DAVELFFLQIQGSGRIRLPDGSLLRVNYADQNGHPYRSIGRLLVDRGDLKLEEASMQG
                     IQAWARANPARLDELLNANPSYVFFREMPASDDGPLGALGVPLTAERSIAIDPRS"
     gene            1041..1922
                     /locus_tag="PLN02_09860"
     CDS             1041..1922
                     /locus_tag="PLN02_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52670.1"
                     /translation="MIDPAQLERLLNRAEHVLSRLEGILPPPPAQPDWAGAVAFRWRK
                     SGGRGWLQGVAHPHPIRLADIETVDDQKARIVANTRQFVAGHGANNVLLTGARGCGKS
                     SLVKAVLNEFSAAGLRLIEVDKHDLVDLPDIVDLVDGRPEKFIIFCDDLSFEAGETAY
                     KALKSVLDGSVAAPPANVLIYATSNRRHLMPEYMAENLEVQRVGDEIHPGEAVEEKVS
                     LSERFGLWISFYPFSQDDYLAVFAHWARQFGVAAAVIAGSEREALNWALSRGSRSGRV
                     AWQFARDLAGRQPEASA"
     gene            1919..>2377
                     /locus_tag="PLN02_09865"
     CDS             1919..>2377
                     /locus_tag="PLN02_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOI52671.1"
                     /translation="MSDIVEVAAAVMLRAGGSEFLLAQRPEGKVYAGYWEFPGGKVEP
                     GESVRDALVRELQEELGITVTACHPWLTRRHVYPHATVRLNFWQVTAWQGEIGITAPL
                     EHSAVDWQRRGAAASVRAEAIQAGATRIESCAEAGDLAAAAQALAALDAHF"
BASE COUNT          347 a          803 c          852 g          375 t
ORIGIN      
        1 atcgaacgcg ggtcgatggc gatgctgcgt tcggcggtga gcggcacgcc cagtgcgccg
       61 agcgggccat cgtcgctggc cggcatttcg cggaagaaga cgtagctggg gttggcgttg
      121 agcagttcgt cgaggcgcgc cgggttggcc cgcgcccagg cctggatgcc ctgcatcgac
      181 gcttcttcca gcttgaggtc gccgcggtcg acgagcaggc ggccgatcga gcggtagggg
      241 tggccgttct ggtcggcgta attgacccgc agcaggctgc cgtcgggcag acggatgcgc
      301 ccggagccct ggatctgcag gaagaagagt tcgaccgcgt catcgaccca ggccagcgtc
      361 ctggccggca ggcgcttgcc ctgggcggcg atctcgccgc gcgaccagta ggggacgacc
      421 ttgttgcctt cgatccggcc gcgcacgcgc ttgcccttga gttccgggaa gaggtcgccg
      481 agttcgatgg tcagcaggtt gtccggcacg ccatgcaccg ggtggaggtt gcgcttgtcg
      541 cgctggcggc tgccctgcag caacggttcg tagtagccgg tgatcatgcc ttcgacggcg
      601 ctgcggtcgt cggtcgatac cgtgtagggc tgcatgacgt tctcgaagta gcggcggatt
      661 gcctcgccgg aacgggcgtc gacctcggcg gcgtgggcgc aggcctgttg ccagggggcg
      721 ccgccggcgc ggctgcgcag cgcctggcag cttttcagga agggttgcca ggcggcgctc
      781 aggtcgtctt cctgccagcc cggcaggtcg ctccaggcgg ccagcgtgaa cacccggctg
      841 aacgccgggg cggccggctt ttccgcagcg gcctcggcac agggcgggca gtccggcgcc
      901 ttggccgcgc cttccggcga aacggtacgc ggcggcacgg cacaggcggc cagcgccagg
      961 gtaacgacga gaacggcggt ttggagtttt gacatggctt tggttagagt gagcaacttt
     1021 tcccgagtat acctgtgacg atgatcgatc ccgcccaact cgaacgtttg ctgaaccgcg
     1081 ccgaacacgt cctgtcccgc ctcgaaggca tcctgccgcc gcccccggca cagcccgact
     1141 gggccggtgc ggtggctttc cgctggcgca agagcggcgg ccgcggctgg ctgcaggggg
     1201 tggcccaccc gcacccgatc cgcctcgccg acatcgagac ggtcgacgac cagaaggcgc
     1261 gcatcgtcgc caacacccgc cagttcgtcg ccggccacgg cgccaacaac gtgctgctga
     1321 ccggcgcccg cggctgcggc aagtcgtcgc tggtgaaggc ggtgctcaat gaattttcgg
     1381 ccgccggcct gcgcctgatc gaggtcgaca agcacgacct ggtcgacctg ccggacatcg
     1441 tcgacctggt cgacggacgg cccgagaagt tcatcatctt ctgcgacgac ctctcgttcg
     1501 aggccggcga aaccgcctac aaggcgctga agtcggtgct cgacggttcg gtcgcggcgc
     1561 cgccggccaa cgtgctgatc tacgccacct ccaaccgccg ccacctgatg cccgaataca
     1621 tggcggaaaa cctggaggtg cagcgtgtcg gcgacgaaat ccaccccggc gaggcggtcg
     1681 aggagaaagt ctcgctgtcc gaacgcttcg gcctgtggat ttccttctac cccttctcgc
     1741 aggacgatta cctggcggtc ttcgcgcact gggcgcggca gttcggtgtc gccgcggcgg
     1801 tgatcgccgg cagcgagcgc gaggcgctga actgggcgct gtcgcgcggt tcgcgctcgg
     1861 gccgggtggc ctggcagttc gcccgcgacc tcgctggccg ccagccggag gccagcgcgt
     1921 gagcgacatc gtcgaagtcg cggcggcggt gatgctgcgc gccggcggca gcgagttcct
     1981 gctcgcccag cgcccggagg gcaaggttta cgccggttac tgggagtttc ccggcggcaa
     2041 ggtcgaaccc ggcgaaagcg tgcgcgacgc gctggtgcgc gaactgcagg aagagctggg
     2101 gatcaccgtc accgcctgcc acccctggct gacccgccgg catgtctatc cgcacgccac
     2161 ggtacgcctc aatttctggc aagtcaccgc ctggcagggc gaaatcggca tcaccgcgcc
     2221 gctcgaacac agcgcggtgg actggcagcg gcgcggcgcg gcggccagcg tgcgcgccga
     2281 agccatccag gccggcgcga cgcgcatcga atcctgcgcc gaggcgggcg atctcgccgc
     2341 cgccgcgcag gcgctggccg cgctcgacgc gcacttc
//
LOCUS       DAOSDX010000444         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15608_length_2375_cov_3.234483, whole genome shotgun sequence.
ACCESSION   DAOSDX010000444 DAOSDX010000000
VERSION     DAOSDX010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15608_length_2375_cov_3.234483"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PLN02_09870"
     CDS             complement(<1..144)
                     /locus_tag="PLN02_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOI52672.1"
                     /translation="MTRKIALITGGSRGLGRNAAVKLAARGVDVVLTYRQRADEATDA
                     VAAI"
     gene            256..1173
                     /locus_tag="PLN02_09875"
     CDS             256..1173
                     /locus_tag="PLN02_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018607727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI52673.1"
                     /translation="MNPLDAMQVFVRVAEVASFTRAADSLGVPKASVSLAVQQLESRV
                     GARLLQRTTRRVQLTPDGQAYLARCLDLLADMDELDQMFRDDESGVHGRLRVDLPLAV
                     ARDLVMPHLPEFLARHPGIALELGSTDRRVDLIGEGFDCVLRVGPLADSSLVARHLGY
                     FRQSNCASPAYLERHGVPASPEALLAGHRIVHYSSTLGDKPAGFEFIDPASGELREQR
                     LPASLVVNNSDAYRAACLAGLGIIQAPVHGMQSLLDAGRLVEILPGYRAPALPVTLLY
                     GNRRHLAKRVRLFMNWLAEVLRPSLSELP"
     gene            1290..1667
                     /locus_tag="PLN02_09880"
     CDS             1290..1667
                     /locus_tag="PLN02_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOI52674.1"
                     /translation="MFAIHVDDTPVITVAGSRHEVCFAMNGSEMNPLEGFYATLAGCA
                     AVYAKKACMEMGVPAAGIEIDCKPFAGSKGPMSLAKFKTEVRFPGHFSAEQRAAVLES
                     IGQCAVKKIVQLGGDIDFVVSEA"
     gene            complement(1726..1944)
                     /locus_tag="PLN02_09885"
     CDS             complement(1726..1944)
                     /locus_tag="PLN02_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HOI52675.1"
                     /translation="MSVANIKAFSDKARADAALGAALKACQKLKELRSLAREHGFEFD
                     EDSLYPPNEPQFTHDQLSERLVKNLLPS"
     gene            complement(2090..>2375)
                     /locus_tag="PLN02_09890"
     CDS             complement(2090..>2375)
                     /locus_tag="PLN02_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52676.1"
                     /translation="VLPELSAMLAEYDSQRMETPNVDQWFLGERVWGYLQHSCLIHDR
                     CFDQPGSRRLPTPGDDRHIGQNEAVLALQERLLRPWIERHPCLGELPPAT"
BASE COUNT          385 a          660 c          819 g          511 t
ORIGIN      
        1 gatggcggcg acggcatcgg tggcttcgtc ggcgcgctgg cgataggtga ggacgacatc
       61 aacgccgcgg gcggcgagct tgactgcggc gttacggccg aggccgcgac tgccgccggt
      121 gatcagagcg atttttcggg tcatggtgca ctcctggggt ggttgaacga tggctgcagt
      181 ctattgctgg catcttgtcg aataaactgg cgaattttgt ttagactgtt cgcttattct
      241 ggataaaggt gagcgatgaa cccgctcgat gcgatgcagg tttttgtccg ggtcgccgag
      301 gtggccagtt tcacccgtgc tgcagacagc ctgggggtgc ccaaggcctc ggtctcactg
      361 gccgtgcagc agctggaaag ccgggttggc gcgcggctat tgcagcgaac gacgcggcgc
      421 gtccaattga cgcccgacgg tcaggcttat ctcgcgcgct gcctggatct gctggccgac
      481 atggacgaac tcgatcagat gttccgcgat gatgagagcg gtgtgcatgg acggctgcgc
      541 gtcgacctgc cgttggcggt ggcgcgcgat ctggtgatgc cgcacttgcc ggaattcctc
      601 gcccggcatc cggggattgc ccttgagctg ggcagtaccg accgccgcgt cgacctgatc
      661 ggcgagggct tcgattgcgt gctacgcgtc gggccgctcg ccgactcgtc attggtcgcc
      721 cgccatctcg gctatttccg ccagagcaac tgcgccagcc cggcctatct ggaacgtcac
      781 ggcgtgccgg cctcgcctga ggcgctgctg gccggccacc ggatcgtcca ttacagcagt
      841 acgctgggcg acaagcccgc cggcttcgag ttcatcgatc cggccagcgg cgaattgcgc
      901 gagcagcgcc tgccggccag cctggtggta aacaattccg atgcctatcg cgccgcctgc
      961 ctggccgggc tcggcatcat ccaggcgccg gtgcatggca tgcagtcctt acttgatgcc
     1021 gggcggttgg tcgaaatcct gcccggatac cgggcgccgg cactgccggt gacgctgctc
     1081 tacggcaacc ggcgccatct ggccaaacgc gtgcggttgt tcatgaactg gctggctgag
     1141 gtgttgcgac cgtcgctgag cgagttgccc tgatctcgcg ctgcgatgtt gcactgcagc
     1201 aggcggctga tttaaacttt agtgtataag aatttcctga tttacaaggc tgcctggacg
     1261 cggcggccat ttccctcgga gaaaaaagca tgtttgccat acacgtcgac gatacgccgg
     1321 tgatcacggt tgccggcagt cgccatgaag tctgttttgc gatgaacggg tcggagatga
     1381 atccgctcga aggtttttac gccacgctgg ccggttgcgc ggcggtctat gccaagaagg
     1441 cctgcatgga aatgggggtg ccggcagcgg gcatcgagat cgattgcaag cctttcgccg
     1501 ggagcaaagg gccgatgtcg ctggccaaat tcaagacgga ggttcgtttc cccgggcatt
     1561 tttcggccga gcagcgggcc gctgtgcttg aatcgatcgg tcagtgtgca gtgaagaaaa
     1621 tcgttcagct cggtggcgat atcgattttg tcgtcagcga agcctgaacc ttcctgttct
     1681 tgccgtgaaa aaagccggga ttttcccggc ttttttgttg gccgatcagc tcggcagcag
     1741 gttcttgacc aggcgttcgg aaagctggtc gtgggtgaat tgcggttcgt tcggcgggta
     1801 gagcgagtct tcgtcgaatt cgaagccgtg ctcgcgggcg aggctacgca gttccttgag
     1861 tttctggcag gccttgaggg ctgcgccgag ggcggcatcg gccctggctt tatcggaaaa
     1921 tgccttgatg ttggcaacgg acatcggggt tttctcctgt gcggttgtag tggtgaacga
     1981 gggtgtcgct cggccgtctt gtgtcgggtt gtcgacagaa aaggcagcat tgagcgatcg
     2041 gaaaaccctg taagcaattt ccgcgccgtt cggcgggggc tgctggcgct caggttgccg
     2101 gtggcagttc gccaaggcag gggtggcgtt cgatccacgg gcgcagcagg cgctcctgga
     2161 gggcgagcac ggcttcgttc tggccgatgt ggcggtcgtc gccgggcgtt ggcaggcggc
     2221 ggctgcctgg ctggtcgaag cagcggtcgt ggatcaggca actgtgctgg agatagcccc
     2281 agacccgttc gccgagaaac cattggtcga cgttcggggt ttccatgcgt tgcgaatcgt
     2341 actcggcgag catggcggac agttcgggca ggacg
//
LOCUS       DAOSDX010000445         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15611_length_2375_cov_2.752586, whole genome shotgun sequence.
ACCESSION   DAOSDX010000445 DAOSDX010000000
VERSION     DAOSDX010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15611_length_2375_cov_2.752586"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLN02_09895"
     CDS             complement(<1..291)
                     /locus_tag="PLN02_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOI52677.1"
                     /translation="MKRSLLIGCLAAAFSLPLTAHEGHDHGDEKAALPNLGNTPQRLA
                     DGTVFLPKTAQRQLGVRTAPVAAADAARAVELSGQVVIDPQRGGRVQTMLAGR"
     gene            complement(288..803)
                     /locus_tag="PLN02_09900"
     CDS             complement(288..803)
                     /locus_tag="PLN02_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52678.1"
                     /translation="MKLKNLLIAGATVAVLLPAQAAEPVVPLPVVVAESEAFEIVGRL
                     DENSLLFHVDRAPTNAPVLGATLSVEAGGKEVAAPFDAASGAYRISDAAWLQPLRAVG
                     EHALSFTLLAGDEADLLAASLSVVAEAPPAVAAGWRLSPLGGGLLLVGIGLGGAVLVW
                     RRIKQRQGGVA"
     gene            complement(809..2032)
                     /locus_tag="PLN02_09905"
     CDS             complement(809..2032)
                     /locus_tag="PLN02_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOI52679.1"
                     /translation="MRPVARILVLLALLPVTPVGAETVVTLPQAFAAAWTRHSLSQTL
                     PLQREAVAARRDAAAAWTPEPAAIEIGGRSDRFNADRGAQEVELGLALPLWLPGERQA
                     SQALAEAEGARLEGRAAAQRLQLAGELRSAWWEWRLARDEVALSDNALDSARRLRDDV
                     ARRVAAGDLARADLHQAAAALASAQAEQADVAVRLEAAAARLQWLTGQRPAALNDDGE
                     AAPAKVAEADWLSAHPELQAAQARFEQARQAQALARSRSRANPELTVSTRRERGASGE
                     AAERSWSLGLRIPLSSSAGHGAAIAEAGAERVEAEIDVERERERLQQTAQLARSQWQA
                     AERRRQASEERAQLARETQRFFDKAFRLGESDLPTRLRVAQETFAAERAASRARIQES
                     AALSAYRQALGLLPE"
     gene            complement(2129..>2375)
                     /locus_tag="PLN02_09910"
     CDS             complement(2129..>2375)
                     /locus_tag="PLN02_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52680.1"
                     /translation="QMHAAMDDDCDACPDHDQTPAPCDDVQHQCCPGHLSGYPATPAV
                     VALLPAPAATPVVDRRPDGFPSRVPEGLERPPRTVSA"
BASE COUNT          353 a          821 c          850 g          351 t
ORIGIN      
        1 gcgaccggcc agcatggtct ggacgcgacc gccgcgctgc gggtcgatga cgacctgacc
       61 ggacaattcg accgcccgcg ccgcgtcggc ggcggccacc ggcgcggtac gcacgccgag
      121 ctggcgctgc gccgtcttgg gcaggaaaac ggtgccatcg gccagccgct gcggcgtgtt
      181 gccgaggttg ggcaaagcgg ccttttcatc gccatggtca tgcccctcgt gcgccgtcag
      241 cgggagggaa aacgcggctg ccaggcagcc gatcagaaga ctgcgtttca tgccacacct
      301 ccctgccgtt gtttgattcg gcgccacacc aggacggcgc cgcccagacc gatcccaact
      361 agcagcaggc cgccgcccag cggcgacaga cgccagccgg ccgcgacggc cggcggcgct
      421 tcagccacaa cgctcaagct ggcggccagc aggtcggctt cgtcgccggc cagcaaggta
      481 aaggacagcg cgtgttcgcc cactgcccgc agcggctgca accaggcggc atcgctgatc
      541 cggtaagcgc cgctggcggc gtcgaagggc gcggcaactt ccttgccgcc ggcctcgacc
      601 gacagcgtcg caccgagcac tggcgcgttg gtcggcgccc ggtcgacatg gaaaagcagc
      661 gaattttcat cgagtcggcc gacgatttcg aaggcctcgc tctcggcgac gaccaccggc
      721 agcggcacga cgggttcggc ggcctgggcc ggcagcagca cggctacggt ggcgccggca
      781 atcagtaggt ttttcaattt catcgtgttc attccggtaa caggccgagg gcctgacgat
      841 aggcggacag cgcagccgat tcctggatgc gcgcgcggct ggcggcgcgt tcggcggcga
      901 aagtttcctg ggcaacgcgc agacgcgtcg gcagatcgct ttcgccgagg cggaaagcct
      961 tgtcgaaaaa gcgctgcgtc tcacgcgcca gttgcgcccg ttcttcgctc gcctgacggc
     1021 ggcgttcggc cgcctgccac tggctgcggg caagctgcgc cgtctgttgc aggcgctcgc
     1081 gctcgcgttc cacgtcgatt tcggcctcga cgcgctcggc gccggcttcg gcaatcgccg
     1141 cgccatggcc agccgacgag gaaagcggaa tgcgcagccc gagcgaccag ctgcgttcgg
     1201 cggcctcgcc gctggccccg cgctcgcgcc gagtggacac ggtcagttcg ggattcgccc
     1261 gactgcgcga tcgggccagc gcctgcgcct gacgcgcctg ttcgaagcgg gcctgggcgg
     1321 cctgcagttc aggatgggcg ctcagccagt cggcctcggc caccttggcc ggggccgctt
     1381 cgccgtcgtc gttcagggcc gcgggccgct gcccggtcag ccactgcagg cgggctgccg
     1441 cggcttccag gcgaaccgcg acatcggcct gctcggcttg cgccgaggct agagcggccg
     1501 cagcctggtg caggtcggcg cgcgccagat cgccggcagc gacccgccgc gccacgtcgt
     1561 cgcgcagccg cctggcactg tcgagcgcat tgtcgctcag cgcgacttcg tcgcgggcga
     1621 gccgccattc ccaccaggcg ctgcgcagtt caccggccag ttgcaggcgt tgcgccgccg
     1681 cccgtccttc gaggcgggca ccttcggcct cggccagcgc ctggctggcc tgacgctcac
     1741 cgggcagcca cagcggcaag gccagaccga gttcgacttc ctgggcaccg cgatcggcgt
     1801 tgaaacggtc gctccgtccg ccaatttcga tggctgccgg ctccggggtc caggccgcgg
     1861 cggcgtcccg ccgggcggca accgcctcgc gctgcaatgg cagggtttgc gacagggaat
     1921 gtcgcgtcca ggccgcggcg aacgcctgcg gcaaggtgac gactgtttcg gcgccgaccg
     1981 gggtgaccgg tagcagcgcc agcaagacaa ggattcgcgc aacagggcgc atgggaaatc
     2041 tccggctgga tggaacaagc cggcgagttg ccgttttcgg ctcgcgagga gcctggacta
     2101 cagggagtta acggtcagcc gccgacgctc aggcggagac ggtgcggggt ggtcgttcga
     2161 gaccttcggg gacgcgcgac ggaaaaccgt ccggtcggcg gtcgacgacc ggcgtggcgg
     2221 ccggtgccgg aagcaaggcg acgacagcgg gggttgccgg gtagccggac agatgtccgg
     2281 gacagcattg gtgctggaca tcgtcgcagg gcgccggtgt ctgatcgtga tcgggacaag
     2341 cgtcgcaatc atcgtccatc gcggcatgca tctgc
//
LOCUS       DAOSDX010000446         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15652_length_2369_cov_3.079948, whole genome shotgun sequence.
ACCESSION   DAOSDX010000446 DAOSDX010000000
VERSION     DAOSDX010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15652_length_2369_cov_3.079948"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="bamA"
                     /locus_tag="PLN02_09915"
     CDS             complement(<1..908)
                     /gene="bamA"
                     /locus_tag="PLN02_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOI52681.1"
                     /translation="MKKSLLPALIAGLFAMPALAFSPFVVKDIRVEGIQRTEAGTVFS
                     YLPVKVGETFTDEKAAQAIKSLFATGFFKDVRIDVEGNVMVVVLQERPAIASLDFVGM
                     KEFEKDAIIKALKEVGIATGRIFDRSLLDKAEQELKRQYLTRGKYAARITTTVTPLER
                     NRVGVNFTIVEGDAAKIRQINIVGAKAFPEKELQSLFELTTPGWLTWYNKNDQYSRQK
                     LSADIEKLRSFYLDRGYMEFNVESTQVSISPDKQDVYITLNIVEGERFKVASVKLAGD
                     FTIPEAELRKLVSVRPGDVFSREKLN"
     gene            complement(911..2278)
                     /gene="rseP"
                     /locus_tag="PLN02_09920"
     CDS             complement(911..2278)
                     /gene="rseP"
                     /locus_tag="PLN02_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOI52682.1"
                     /translation="MSDLPLTLAAFALALGILIVVHEFGHYIVARWCGVKVLRFSIGF
                     GPVLWQRRLGRDATEWAVSALPLGGYVKMLDEREGEVAAEELPRAFNRQGVGRRSLIV
                     AAGPLANFLLAIFLYWAVFLHGTNELLPVLGAPALSTPAAAAGIRGGDQVMRIDDETV
                     ATWDDFRWQLLQLAASRAQIALDVVDADGVARTRILALDAVREEGWEGDPLVHLGLRF
                     YRPDLPAVIGRLVDGGPGARAGLMEGDRVLAIDGRPLANWQALVLAVREAAGRSLRFD
                     IERQGVSLQLAVTPDEASEGGQRIGRIGVAVAETPESRRELRAFVRYGVIDAGRKAIF
                     ETWDKSIFSLRMMGKMLTGEVSWKNLSGPVTIADYAGQTAKLGIDYYVRFMALISISL
                     GVLNLLPVPVLDGGHLMYHMIEVVRRKPLSERAMAIGQQFGLAILFSLMAFAFFNDLN
                     RLFGG"
BASE COUNT          481 a          767 c          713 g          408 t
ORIGIN      
        1 tcgttaagct tttcccgcga gaagacatcg ccgggacgca ccgaaaccag cttgcgcaat
       61 tccgcttcgg gaatcgtgaa atcgccggcc agcttgaccg aggccacctt gaagcgttcg
      121 ccctcgacaa tgttgagggt gatgtagacg tcctgcttgt cgggcgaaat cgagacctgg
      181 gtggactcga cgttgaattc catgtaaccg cgatccaggt agaaggaacg cagtttttcg
      241 atgtcggcgg aaagcttctg gcgggaatac tggtcgttct tgttatacca ggtcagccag
      301 cccggcgtgg tcaactcgaa cagcgactgc agttccttct ccgggaaggc cttggcgccg
      361 acaatgttga tctggcgaat cttcgcggca tcgccttcga cgatggtgaa gttgacgcca
      421 acccgattgc gttcaagcgg cgtcaccgtc gtcgtgatcc gggcggcata cttgccgcgc
      481 gtcaggtact gccgcttgag ctcctgctcc gccttgtcga gcaaggaacg gtcgaagatg
      541 cggccagtgg cgataccgac ttccttgagc gccttgatga tcgcgtcttt ctcgaattcc
      601 ttcatgccga cgaaatcgag cgaggcgatc gccggccgct cctgcaggac gacgaccatg
      661 acattgcctt cgacgtcgat ccgcacatcc ttgaagaaac cagtggcgaa cagcgacttg
      721 atggcctgtg ccgctttttc gtcggtgaag gtttcgccga ccttgaccgg cagataactg
      781 aacaccgtcc ccgcctcggt acgctggata ccctcgacac ggatgtcctt gaccacgaac
      841 ggggaaaagg ccagcgcggg catggcaaac agaccggcaa tcagggctgg caacagggat
      901 tttttcatcg ttagccgccg aacaggcgat tcaagtcgtt aaaaaatgcg aaagccatca
      961 agctgaacaa aatggcgagt ccgaactgtt gtccgattgc cattgcccgc tccgagaggg
     1021 gcttccgcct gacgacttcg atcatatgat acatcaaatg cccgccatcc agcaccggga
     1081 cgggaagcag gttgagcacc cccaggctga tgctgatcag cgccatgaaa cggacataat
     1141 aatcgatccc gagcttcgcc gtctgtccgg cgtaatcggc gatcgtcacc ggccccgaca
     1201 ggtttttcca ggaaacctcg ccggtcagca tcttgcccat catgcgcagg ctgaagatcg
     1261 acttgtccca ggtttcgaag atcgccttgc gccctgcgtc aatgacgccg taacggacga
     1321 aggcacgcag ttcgcgacgg ctttccgggg tttcggcaac cgccacgccg atgcggccga
     1381 tccgctgacc gccttcggaa gcttcgtctg gagtgacagc aagctgcaag ctgacgccct
     1441 gccgctcgat gtcgaaacgc aggctgcgcc ctgccgcttc acggacggca agcaccagcg
     1501 cctgccagtt ggcgagcgga cgaccgtcga tcgccagcac gcggtcgccc tccatcaagc
     1561 cggccctggc accgggtccg ccatcgacca ggcggccgat gaccgccggc aggtccggac
     1621 gatagaaacg cagaccaaga tgcaccagag gatcgccctc ccagccctct tcccgcaccg
     1681 catcgagcgc caggatacgc gtgcgagcga cgccgtcggc atcgacgaca tcaagtgcga
     1741 tttgcgcgcg cgatgcagct agctgcagga gttgccagcg aaaatcgtcc caggtcgcca
     1801 ccgtctcgtc gtcgatgcgc atcacctgat cgccgccccg gataccggcg gcggcggcgg
     1861 gcgtcgagag agccggcgca ccgaggacgg gcagcagttc gttggtgccg tgcaggaaaa
     1921 ccgcccagta caggaagatg gccagcagga aattggccag cggaccggca gcaacgatca
     1981 ggctgcgccg accgaccccc tgccggttga aggcacgcgg cagttcttcc gccgccacct
     2041 cgccttcccg ttcgtcgagc atcttgacgt agccgcccag cggcagggcg ctgaccgccc
     2101 actccgtggc atcgcgcccg agacgccgct gccagagaac ggggccgaaa ccgatcgaga
     2161 agcgcaagac cttgacgccg caccagcgcg cgacgatgta atgcccgaat tcatggacga
     2221 cgatcaggat gcccaatgcc agcgcgaagg cggccagagt cagcggcaga tcgctcatgc
     2281 gaaacgcccg tcggccacca tgacgcccgc caactgccgc gcccgggcgt cggcggcgat
     2341 gacgtcgtcg aggctgccct cgggaccgg
//
LOCUS       DAOSDX010000447         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15678_length_2366_cov_3.071830, whole genome shotgun sequence.
ACCESSION   DAOSDX010000447 DAOSDX010000000
VERSION     DAOSDX010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15678_length_2366_cov_3.071830"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..650
                     /gene="dksA"
                     /locus_tag="PLN02_09925"
     CDS             219..650
                     /gene="dksA"
                     /locus_tag="PLN02_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOI52683.1"
                     /translation="MSRSTAKPGLPSEAELLSASEDEYMSPTQLAFFRQRLLDEEAAL
                     RGAAEETTHHLQETAAEPDPNDRASAEEEHALELRVRDRERKLLKKIDQALKRIDDGS
                     YGWCEDTGEPIGIARLLARPTASLCLEAQERHEQRRRQFGG"
     gene            702..1208
                     /locus_tag="PLN02_09930"
     CDS             702..1208
                     /locus_tag="PLN02_09930"
                     /inference="COORDINATES: protein motif:HMM:NF022439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HOI52684.1"
                     /translation="MKKSPLLLLALLALQAAAHEQHAHQHGLGQLGIVVEGAQLTLLL
                     EAPQHDLVGFERAARSPREQLAAQAALEKLRAPAQLFVPTAAAQCALSEQKVTAPLLD
                     GQGGSDGHGDIEARYVYLCANPLALDGLRVQVGEAFPRLRALEVSFAGPKGQKAGRVD
                     IRQPVFSW"
     gene            1211..1879
                     /locus_tag="PLN02_09935"
     CDS             1211..1879
                     /locus_tag="PLN02_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI52685.1"
                     /translation="MAIRIDDLLFRWRAAAPPAVRLEHLAVAAGRSLFLHGPSGAGKS
                     TLLNLIGGVLQPERGRIELLGQALGALSAARRDAFRADHLGFIFQQFNLLPWLSARDN
                     VLLPCRFSRRRRQRADDPAAAAARLLAALDLEPAATAAPAGELSVGQQQRVAAARALI
                     GRPEIIVADEPTSALDAARQDAFIELLLGEARAAGSALVFVSHDLRLARHFDDSLALA
                     GGEA"
     gene            1876..>2366
                     /locus_tag="PLN02_09940"
     CDS             1876..>2366
                     /locus_tag="PLN02_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI52686.1"
                     /translation="MNALWRLALASAWNRRLSLGLTLLAVLLATTLLLSVERIRQAAR
                     DGFAQSVSGTDLVVGARSSPVQLVLYSVFRLGEATNNLSWDSYRKIATDPAIAWSVPL
                     ALGDSHRGYPVLGTTPAYFDHYRYGDRQPLRFASGQVFSGIFDAVLGAEVAQRLGYRI
                     GDQ"
BASE COUNT          349 a          803 c          828 g          386 t
ORIGIN      
        1 gccctgttgc cggcagcggg tttcggcgcg ctcgagcaac tcggcttcgg catcgggttc
       61 aatcattctc gaagtcgact tggtggtggg gatgcgatgt tataacatcg cccgtccgca
      121 atcgatagcg ttgcggtcgg gtgcgcagcg tcggttcggg ttggccgtgc cggttgcggg
      181 catgccgcag acgtcccatg acgaacaagg aaagatcgat gtcacgatcc actgcaaaac
      241 cggggttgcc gagcgaggcc gagctgctgt cagcatcgga agatgaatac atgtcgccga
      301 cccagctggc cttcttccgc cagcgcctgc tcgacgagga ggcggcgctg cgcggtgctg
      361 ccgaagagac tacgcatcac ctgcaggaga cggcggccga gcccgatccc aacgatcgcg
      421 ccagcgccga ggaggagcat gcgctggaac tgcgcgtgcg cgatcgcgag cgcaaactgt
      481 tgaagaagat cgaccaggcg ctgaagcgca tcgatgacgg cagctatggc tggtgcgagg
      541 ataccggcga gccgatcggc atcgcccgcc tgctggcgcg gccgaccgcc agcctgtgcc
      601 tggaggcgca ggaacggcac gaacagcggc ggcgccagtt cggcggttga ggccggcggt
      661 tggtccgcgg agcagcgttt cgaaccaggg aggaatagcc gatgaagaaa tcacctctat
      721 tgctgcttgc cctgcttgcg ctgcaagcgg cggcgcatga gcagcatgcc caccagcatg
      781 ggctcggcca gctcggcatc gtcgtcgaag gggcgcagct gacgctgttg ctggaggcgc
      841 cgcagcacga tctggtaggt ttcgagcggg cggcacggag cccacgcgaa caactggccg
      901 cccaggcggc tctggagaag ctgcgcgcgc cggcgcagct tttcgtaccg acagcggccg
      961 cgcagtgcgc cttgagcgaa cagaaggtca ctgccccgct gcttgacggg caaggcggca
     1021 gcgatggtca cggcgatatc gaagcgcgct atgtctatct ttgtgccaac ccgctggcgc
     1081 tcgatggcct gcgggtccag gtcggcgagg cattcccgcg cctgcgcgcg ctcgaagtca
     1141 gttttgccgg cccgaagggg cagaaggccg gtcgcgtgga tatccggcag ccggtcttct
     1201 cttggtaggc atggcgatcc gcatcgacga tctgctgttt cgctggcgcg ccgccgcgcc
     1261 gccagccgtt cgtctcgaac acctggccgt cgccgccggt cgcagtttgt tcctgcacgg
     1321 gccgagcggc gccggcaaga gcaccttgct caacctgatc ggcggcgtcc tgcagcccga
     1381 gcgcggccgc atcgaattgc tcggccaagc gctcggcgcg ctgtcggcgg cgcggcgcga
     1441 cgctttccgc gccgaccatc tcggtttcat cttccagcag ttcaacctgc tgccctggct
     1501 gtcggcgcgc gacaacgtgc tgctgccctg ccgcttttcg cggcgtcgcc ggcagcgcgc
     1561 cgacgatccg gcggccgcgg cggcgcgcct gctggcggcg ctcgacctgg agcccgcggc
     1621 gacggcggcg cccgccggcg aactgtcggt cggccagcag cagcgcgtcg ccgcggcgcg
     1681 cgcgctgatc ggccggccgg aaatcatcgt cgccgacgag ccgacctcgg cgctcgacgc
     1741 cgcccgccag gatgccttca tcgagctgtt gctgggtgag gcgagggcgg ccggcagcgc
     1801 gctcgtcttc gtcagccacg atctgcggtt ggccaggcat ttcgacgaca gcctcgcttt
     1861 ggccggaggc gaggcatgaa cgcactctgg cggctggccc tggccagcgc ctggaaccgt
     1921 cgcctcagcc tggggctgac gctgctcgcc gtgctcctgg cgacgaccct gctgctctcg
     1981 gtcgaacgca tccgccaggc ggcgcgcgac ggtttcgcgc agtcggtttc cggtaccgac
     2041 ctggtcgtcg gcgcccgcag cagcccggtg caactggtgc tgtattcggt gttccggctc
     2101 ggcgaggcga ccaacaacct gagctgggac agctaccgga agatcgccac cgacccggcg
     2161 atcgcctgga gcgtgccgct ggcgctcggc gactcgcacc gcggctaccc ggtgctcggc
     2221 acgacgccgg cctatttcga ccattaccgc tacggcgacc gccagccgct gcgcttcgcc
     2281 agcggccagg ttttctccgg catcttcgac gccgtgctcg gcgccgaggt ggcgcagcgc
     2341 ctgggctacc gcattggcga ccagat
//
LOCUS       DAOSDX010000448         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15695_length_2363_cov_3.384315, whole genome shotgun sequence.
ACCESSION   DAOSDX010000448 DAOSDX010000000
VERSION     DAOSDX010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15695_length_2363_cov_3.384315"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="PLN02_09945"
     CDS             <1..98
                     /locus_tag="PLN02_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52687.1"
                     /translation="IAPIAMEEGLRFAIREGGRTVGAGVVSKIIE"
     gene            165..476
                     /gene="rpsJ"
                     /locus_tag="PLN02_09950"
     CDS             165..476
                     /gene="rpsJ"
                     /locus_tag="PLN02_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOI52688.1"
                     /translation="MQSQKIRIRLKAFDYRLIDQSAQEIVETAKRTGAVVRGPVPLPT
                     RKQRFDILRSPHVNKASRDQLEIRTHLRLMDIVDPTDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            587..1225
                     /gene="rplC"
                     /locus_tag="PLN02_09955"
     CDS             587..1225
                     /gene="rplC"
                     /locus_tag="PLN02_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOI52689.1"
                     /translation="MSLGLVGRKVGMTRIFAEDGASIPVTVLDVSNNRVTQVKTPEVD
                     GYAAVQVAFGQRRASRVSKPLAGHLAKAGVEAGHVLKEFPVTAEQLANFKAGDQVAVT
                     IFAEGQKVDVTGTSIGKGFQGGIKRHNFSSNRASHGNSVSHNAPGSIGMAQDPGRVFP
                     GKRMAGHLGDEQSTTQGLTIVRVDAERQLLLVKGAVPGAKGSDVVVRPAVKA"
     gene            1235..1858
                     /gene="rplD"
                     /locus_tag="PLN02_09960"
     CDS             1235..1858
                     /gene="rplD"
                     /locus_tag="PLN02_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOI52690.1"
                     /translation="MELKVINEQGQEAAKLQASDVLFGRDYNEALVHQIVVAYQANAR
                     SADSKQKDRSEVRHTTTKPWRQKGTGRARSGSNGSPLWRGGGRIFPNSPDQNYGQKVN
                     KKMFRAGMAAILSELARQDRLVVIDDLAVEAPKTKLFAQKLKGMGLEGNLLVVTDELN
                     ENLYLSSRNLPNVLVLEAQEADPVSLIRFAKVLVTKNAVAKFEEMWG"
     gene            1855..2157
                     /gene="rplW"
                     /locus_tag="PLN02_09965"
     CDS             1855..2157
                     /gene="rplW"
                     /locus_tag="PLN02_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOI52691.1"
                     /translation="MNQERLMQVLLAPQISEKATFVAEKNEQVIFRVASDATKPEIKA
                     AVELLFKVEVESVQTLNVKGKVKRFKGATGRRKGWKKAFVCLKAGQEINFVEGGNA"
     gene            2157..>2363
                     /locus_tag="PLN02_09970"
     CDS             2157..>2363
                     /locus_tag="PLN02_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOI52692.1"
                     /translation="MALVKVKPTSPGRRAVVQVVNANLHKGKPFAGLVESQSKNSGRN
                     NNGRITVRHQGGGHKRKYRLVDFKR"
BASE COUNT          480 a          683 c          715 g          485 t
ORIGIN      
        1 tgatcgcccc gatcgccatg gaagaaggtc tgcgcttcgc gatccgcgaa ggcggccgta
       61 ccgtcggtgc cggtgtcgtc tccaagatca tcgagtaagc gttcttttgt tcaatccggg
      121 gtggcggccc tcgctgcccc atcgttcttt ttgaaagcca aaaaatgcaa agccagaaaa
      181 tccgtatccg cctgaaggcc ttcgactatc gcctgatcga ccagtccgct caggaaatcg
      241 tcgagaccgc caagcgtacc ggcgccgtcg ttcgtggtcc cgtgccgctg ccgacccgca
      301 agcagcgttt cgacatcctg cgttcgccgc acgtcaacaa ggcttcgcgc gatcagctcg
      361 aaatccgcac ccacctgcgc ctgatggaca ttgttgatcc caccgacaag acggtcgatg
      421 cgctgatgaa gctggacctg ccggctggcg tggacgtcga aatcaagttg cagtaactgt
      481 ggtcttgcgg atataatccg ctgttttcgg gggtggccgg tgacggctgc ccatttgttg
      541 ttttacatcg accggccaat cgcagccggc gatgaggaga ataaccatga gtctaggcct
      601 tgttggtcgc aaggttggca tgactcgcat ttttgccgag gatggtgcgt ccattccggt
      661 aactgtgctt gacgtgtcca acaaccgcgt gacccaagtc aaaacgccgg aagtcgatgg
      721 ctatgcagcc gtccaggtcg ctttcggtca gcgccgtgcc tcccgtgttt cgaagcccct
      781 tgctggccat ctggccaagg cgggcgttga agccgggcac gtgctcaagg aattcccggt
      841 taccgccgag cagctcgcca atttcaaggc gggtgatcag gttgccgtca ccatctttgc
      901 cgaagggcag aaggttgatg tcaccggtac ctcgatcggt aagggtttcc agggcggtat
      961 caagcgtcac aacttctctt ccaaccgtgc atcccacggt aactcggtgt cgcacaatgc
     1021 gccgggttcc atcggtatgg cgcaagatcc ggggcgtgtt ttcccgggta agcgcatggc
     1081 tggccatctg ggcgacgagc agtcgacgac ccagggtttg accatcgttc gcgttgatgc
     1141 cgagcggcaa ctgttgctgg tcaagggcgc cgtgcctggc gccaagggtt ccgacgtcgt
     1201 cgtgcgtccg gcagtcaagg cttaaggggg cgtcatggaa ctgaaggtta tcaacgaaca
     1261 aggtcaggaa gctgccaagc tgcaggcttc cgacgtgctg ttcggtcgcg attacaacga
     1321 ggctctggtt caccagatcg tcgtcgccta tcaggcgaat gctcgttccg ccgacagcaa
     1381 gcagaaggac cgctccgaag tccgtcacac caccaccaag ccgtggcgcc agaagggtac
     1441 cggtcgtgcc cgttcgggtt cgaacggttc gccgctgtgg cgtgggggcg gtcggatttt
     1501 tccgaactct ccggatcaga actacggtca gaaggtcaac aagaagatgt tccgcgccgg
     1561 tatggcagcg atcctctccg aattggcgcg tcaggaccgt ctcgtcgtga tcgacgacct
     1621 cgccgtcgaa gcgccgaaga ccaagctgtt cgcccagaag ctgaagggca tgggtctcga
     1681 aggcaatctg ctggtggtta ccgacgaact gaacgagaac ctctacctgt cgtcgcgcaa
     1741 tctgccgaac gtgctggtcc ttgaagcgca ggaagccgat ccggtttcgc tgatccgctt
     1801 tgccaaggtg ctggtcacca agaatgccgt ggccaaattc gaggagatgt ggggatgaac
     1861 caggaacgtc tgatgcaggt gctgctggca ccgcaaatct ccgaaaaggc gacctttgtt
     1921 gccgagaaga acgagcaggt gattttccgt gtcgcctccg atgcgaccaa gccggaaatc
     1981 aaggctgccg tcgaactgct gttcaaggtc gaagtcgagt cggtgcaaac cctgaacgtc
     2041 aagggcaagg tcaagcgctt caagggcgcc accggtcgtc gcaagggctg gaagaaggcc
     2101 tttgtctgcc tcaaggccgg ccaggaaatc aattttgtcg aagggggtaa cgcgtaatgg
     2161 cgctcgttaa agtcaaaccg acttccccgg ggcgtcgtgc tgttgttcag gtggttaacg
     2221 ccaatctgca caagggcaag ccgttcgccg gcctggtcga gtcccagtcc aagaattccg
     2281 gtcgcaacaa caacggtcgc atcaccgttc gccatcaagg cggcggtcac aagcgtaaat
     2341 atcgcctggt cgactttaaa cgc
//
LOCUS       DAOSDX010000449         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15697_length_2363_cov_2.990901, whole genome shotgun sequence.
ACCESSION   DAOSDX010000449 DAOSDX010000000
VERSION     DAOSDX010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15697_length_2363_cov_2.990901"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLN02_09975"
     CDS             <1..478
                     /locus_tag="PLN02_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HOI52693.1"
                     /translation="NDAFAFARDARVLEGTQAVAGLGRLHDLLAEVSGEVVWRLEGFK
                     GDLGEPLLHLTVGGCIPLACQRCLEAIPFELEVDTLLELVPASADLSQDELEDDSRDF
                     LPVEKELDVLELVEDEILLSLPVAPRHEKCGLPGAADAGERINPFAALAGLKGKPN"
     gene            497..676
                     /gene="rpmF"
                     /locus_tag="PLN02_09980"
     CDS             497..676
                     /gene="rpmF"
                     /locus_tag="PLN02_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOI52694.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLTTPPLAVEPTTGETHLRHHISP
                     TGFYRGKKVLKGKGE"
     gene            731..1759
                     /gene="plsX"
                     /locus_tag="PLN02_09985"
     CDS             731..1759
                     /gene="plsX"
                     /locus_tag="PLN02_09985"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOI52695.1"
                     /translation="MTTRIAIDCMGGDHGPSVTVPAAIRFLAEHPAANLLLVGQEEVL
                     RPLLGAAAGDSRLRIVHASEIVGMDESPALALRNKKDSSMRVAINLVKSGDADACVSA
                     GNTGALMAISRFVLKMLPGIDRPAICAPLPTLSGHTHMLDLGANVDCGPEHLLQFGIM
                     GAMLVAAMEHKERPTVGILNIGEEEIKGNEVVKAAAELLRNSGLNFIGNVEGDGIYQG
                     EADVIVCDGFVGNVALKTSEGLAQMLASSLRSEFKRNWLTKIAALIAISVLNNFKKRF
                     DHRRYNGAILLGLKGISVKSHGSADALSFGYAIARAFDAAENRALERISDRLAQTNAD
                     RMVAVENA"
     gene            1762..>2363
                     /locus_tag="PLN02_09990"
     CDS             1762..>2363
                     /locus_tag="PLN02_09990"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HOI52696.1"
                     /translation="MYARVTGTGSFLPGNPVSNNDLAARGIDTSDEWIASRTGIRSRH
                     LAEAGTTSSQLGLIAAQRALEMAGVAAEEIDLIIVATSTPDFIFPSTACIIQGLLGNK
                     GAAAFDVQAVCAGFTYALGIAEKFIAAGSHRKALVIGAEVFSRILDWNDRGTCVLFGD
                     GAGAVVLEAAETPGIMASALHADGSQFAILNVPGQISNGQV"
BASE COUNT          432 a          728 c          751 g          452 t
ORIGIN      
        1 caacgacgct tttgcctttg cccgggatgc gcgcgttctt gaaggaacgc aggcggttgc
       61 tggcttgggg cgcctgcatg atttgctggc tgaagtgtcg ggtgaggtcg tctggcgcct
      121 ggaaggtttc aagggtgatc ttggcgagcc gctgctgcat ctgacagtcg gtgggtgcat
      181 cccgctggcg tgtcagcgct gcctggaagc cattcctttt gagttggagg tcgataccct
      241 gctggaactc gttccggcga gtgccgacct gtcgcaagac gaactggaag acgacagccg
      301 ggattttctt ccggtggaga aggaactgga tgtgctggaa ctggttgagg acgaaatcct
      361 gttgtccctg ccggttgcac cacgacatga aaaatgtggc ctgcctggtg cggccgacgc
      421 tggcgagcgg atcaatccgt tcgccgcgct ggccgggctc aaaggcaagc cgaactgatt
      481 tttttggagt aacgaaatgg ccgttcaaca gaacaagaag tccccgtcca agcgtggcat
      541 gcatcgcgct cacgatttcc tgaccacccc gccgctcgcc gtcgagccga cgaccggtga
      601 aacgcacctg cgtcaccaca tttccccgac cggtttctac cgcggcaaga aagtcctcaa
      661 gggcaagggc gagtaatcgt ctcgtaaagg gcaggtggct ccttcgggag agcctgcctt
      721 ttcttttgcc atgacaaccc gcatcgccat tgattgcatg gggggtgacc acggtccgtc
      781 ggtgacggtg ccggcggcca tccgttttct tgcagagcat ccggcggcaa atcttctcct
      841 ggttggccag gaagaagtct tgcgtccctt gctcggcgct gccgccggcg attcccgcct
      901 gcgcatcgtg cacgcttccg aaatcgtcgg catggacgaa tcgccggcgc tcgccctgcg
      961 caacaagaag gattcgtcga tgcgcgtggc gatcaacctc gtcaagtccg gcgacgctga
     1021 tgcctgcgtc tcggccggca acaccggcgc cttgatggcg atctcgcgct tcgtcctgaa
     1081 aatgctgccg ggtatcgacc ggccggccat ctgtgcgccg ctgccgacgc tgagcggcca
     1141 cacgcacatg ctcgacctcg gcgccaatgt cgattgcggc cccgagcatc tgctgcagtt
     1201 cggcatcatg ggtgccatgc tggtcgcggc gatggagcac aaggagcggc cgacggtggg
     1261 catcctcaac atcggcgaag aggaaatcaa gggcaacgaa gtggtcaagg cggcagccga
     1321 actgctgcgc aactccggcc tgaacttcat cggcaacgtc gaaggcgacg gcatctacca
     1381 gggcgaggca gacgtcatcg tctgcgacgg ctttgtcggc aacgtcgccc tgaagacctc
     1441 cgaagggctg gcgcagatgc tggcgtcgtc gttgcgtagc gagttcaagc gcaactggct
     1501 gaccaagatc gcggcgctga tcgccatctc ggtactcaac aatttcaaga aacgtttcga
     1561 ccaccgtcgt tacaacggcg cgatcctgct cggtctgaag gggatttcgg tgaagagcca
     1621 cggctctgcc gacgcgctgt cgttcggcta tgccatcgcc cgcgccttcg atgcggccga
     1681 aaaccgggcg ctggagcgca tcagcgacag gttggcgcaa acgaatgcgg accgtatggt
     1741 cgcagtggag aatgcctgag catgtacgca cgagtcaccg gtaccggcag ctttctgccg
     1801 ggcaatcccg tcagcaataa tgatctggcg gcgcgcggca tcgacaccag cgatgaatgg
     1861 atcgccagcc gcaccggcat ccgtagccgc catctggcgg aagcagggac gacatccagc
     1921 cagctcggcc tgatcgccgc gcagcgggcc ctcgaaatgg ccggtgttgc cgcggaagaa
     1981 atcgacctga tcatcgtcgc cacgtcgacg ccggatttca tctttcccag caccgcctgc
     2041 atcatccagg gcctgctcgg caacaagggc gccgccgcct tcgacgtgca ggcggtttgt
     2101 gccggcttca cctatgcgct gggaattgcc gagaaattca tcgctgcggg cagtcatcgc
     2161 aaggcgctgg tgatcggcgc cgaggtcttc tcgcgcattc tcgactggaa tgatcgcggc
     2221 acctgcgtcc tgttcggcga cggtgccggc gccgtggtgc tcgaagctgc ggagacgccg
     2281 ggcatcatgg ccagcgcgct gcatgccgac ggtagtcagt tcgccatcct caacgtcccc
     2341 ggccagatca gcaatggcca ggt
//
LOCUS       DAOSDX010000450         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15707_length_2361_cov_3.811362, whole genome shotgun sequence.
ACCESSION   DAOSDX010000450 DAOSDX010000000
VERSION     DAOSDX010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15707_length_2361_cov_3.811362"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLN02_09995"
     CDS             complement(<1..571)
                     /locus_tag="PLN02_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI52697.1"
                     /translation="MEMQKLYLLVPMAPLIGAMIAGLFCRVIPRWVAHTATIAGVAIA
                     FVASLFIFQDVMAGNTFNGPVYTWLTSGDYKFEVGFLIDTLTVTMMLVVTFVSLMVHI
                     YTIGYMHDDPGYNRFFSYISLFTFSMLMLVMANNFTQLFFGWEAVGLVSYLLIGFWYT
                     RPTAIFANMKAFLVNRVGDFGFILGIGLVL"
     gene            complement(574..885)
                     /gene="nuoK"
                     /locus_tag="PLN02_10000"
     CDS             complement(574..885)
                     /gene="nuoK"
                     /locus_tag="PLN02_10000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOI52698.1"
                     /translation="MLTLSLSHFLILGAILFAISVVGIFLNRKNLIVLLMAIELMLLA
                     VNMNFVAFSHYLGDLSGQIFVFFILTVAAAEAAIGLAILIVLFRNLKSIHVDDLGSLK
                     G"
     gene            complement(898..1494)
                     /locus_tag="PLN02_10005"
     CDS             complement(898..1494)
                     /locus_tag="PLN02_10005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOI52699.1"
                     /translation="MDFPTAVFFFLSAILVFAALRVITARNPVYAALHLMLAFFTCGG
                     IWALLQAEFLAIAIILVYVGAVMVLFLFVVMMLDINLDRIREGFWQYLPLGAVLGLLM
                     VLEMGLVLGSKYFQVPAAEAVVPAGVSNTKEIGTLMFTDYVFPFELASIILLVGMIAA
                     IVLTYRGPKKSKISNPNTQVFVKAKDRMRVIQMPVEKD"
     gene            complement(1507..1986)
                     /gene="nuoI"
                     /locus_tag="PLN02_10010"
     CDS             complement(1507..1986)
                     /gene="nuoI"
                     /locus_tag="PLN02_10010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOI52700.1"
                     /translation="MMEVFNSLFLKELLKGMSVTGKYFFARKITVQYPEEKTPQSFRF
                     RGLHALRRYENGEERCIACKLCEAICPAMAITIEAEPRDDGSRRTTRYDIDLTKCIFC
                     GFCEEACPVDAIVETRIFEYHGEKRGDLYYTKQMLLANGERYEAQIAEDRALDAPYR"
     gene            complement(2007..>2361)
                     /locus_tag="PLN02_10015"
     CDS             complement(2007..>2361)
                     /locus_tag="PLN02_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOI52701.1"
                     /translation="LAEGESEIVGFHVEYSGMAFALFFLAEYANMILVSALTSLLFLG
                     GWLSPVGFLPDGILCLFATMFFVLVLFLWFRATFPRYRYDLLMRLGWKVFLPLCLIWL
                     VVVGAWMLSPLNIWN"
BASE COUNT          551 a          648 c          770 g          392 t
ORIGIN      
        1 ccagcaccag gccgatgccg aggatgaagc cgaagtcgcc gacgcggttg accaggaagg
       61 ccttcatgtt ggcgaagatc gccgtcggcc gggtgtacca gaagccgatc aacaggtagg
      121 aaacgagacc gaccgcttcc caaccgaaga agagttgggt gaagttgttg gccatcacca
      181 gcatcagcat cgagaaggtg aacagcgaga tgtagctgaa gaagcggttg tagcccggat
      241 catcgtgcat gtagccgatg gtgtagatgt gcaccatcag cgagacgaag gtgacgacca
      301 gcatcatcgt cacggtcagc gtgtcgatca ggaagccgac ctcgaacttg tagtcgccgc
      361 tggtcagcca ggtgtagacc gggccgttga aggtgttgcc ggccatcacg tcctggaaaa
      421 tgaacagcga ggcgacgaag gcgatggcga caccggcgat ggtcgccgtg tgggcgaccc
      481 agcgcgggat gacgcggcag aaaaggccgg cgatcatggc accgatcagg ggcgccatcg
      541 gcacgagcag gtagagtttt tgcatttcca tggttaaccc ttcaggctgc ccaggtcatc
      601 cacgtggatg ctcttgaggt tgcggaacag cacgatcagg atcgccaggc cgatggcggc
      661 ctcggcggcg gcgacggtca ggatgaagaa gacgaagatc tgcccggaga gatcgcccag
      721 atagtgcgag aaggcgacga agttcatatt gaccgcgagc agcatcagtt cgatcgccat
      781 cagcaggacg atcaggttct tccggttgag gaagatgccg accacgctga tcgcgaacag
      841 gatcgcgcca aggatcagga aatgggaaag tgaaagcgtc agcatgttgg ccccgggtca
      901 gtctttttca acaggcatct ggatcacgcg catgcggtcc ttggccttga cgaagacctg
      961 cgtgttcgga ttggaaatct tggatttctt cggcccgcgg taggtcagca cgatggcggc
     1021 gatcatgccg acgagcagga tgatcgaggc gagttcgaag gggaacacgt aatcggtgaa
     1081 catcagcgta ccgatttcct tggtgttcga cacaccggcc gggacgacag cttcggcggc
     1141 cggtacctgg aaatacttgc tgccgagcac cagtcccatc tcgagaacca tcagcaggcc
     1201 gagcacggca ccgagcggca ggtattgcca gaagccctcg cggatgcggt cgaggttgat
     1261 gtcgagcatc atgacgacga acaggaagag caccatgacc gcgccgacat agacgaggat
     1321 gatggcgatc gccaggaact ccgcctgcag cagcgcccag atgccaccgc aggtgaaaaa
     1381 ggcaagcatc aggtgcaagg cggcatacac cgggttgcgc gcagtgatta cgcgcagcgc
     1441 ggcgaacacc aggatcgcgg agaggaagaa gaaaacggca gtcgggaaat ccatcgcggg
     1501 cacctgtcag cggtagggcg catcaagcgc gcggtcttcg gcaatctgcg cttcatagcg
     1561 ctcaccgttg gccagcagca tctgcttggt gtagtagagg tcgccgcgct tttcgccgtg
     1621 gtactcgaaa atgcgcgttt cgacgatcgc gtcgaccggg caggcttctt cgcagaaacc
     1681 gcagaagatg cacttggtca gatcgatgtc gtagcgggtg gtgcgacgcg aaccgtcgtc
     1741 gcgcggctcg gcctcgatgg tgatagccat cgccgggcag atcgcctcgc acagcttgca
     1801 ggcgatgcag cgttcctcgc cgttctcgta gcggcgcagc gcgtgcaggc cacggaaacg
     1861 gaaggactga ggcgtctttt cttccggata ctggacggtg atcttgcggg caaagaaata
     1921 cttgcccgtc accgacatgc ctttcaacag ctccttgagg aagaggctgt tgaagacttc
     1981 catcatcgaa cccattctta ttctcctcag ttccagatgt tcagcgggga cagcatccac
     2041 gccccgacga cgaccagcca gatcaggcac agcggcagga agactttcca gcccaggcgc
     2101 atcagcaggt catagcgata acgggggaag gtggcgcgga accacaggaa aaggacgagc
     2161 acgaagaaca tcgtggcgaa cagacagagg atgccgtcgg gcaggaagcc gaccggcgac
     2221 agccagccgc cgaggaacag cagcgaggtc agcgcggaga ccaggatcat gttcgcgtat
     2281 tcggccagga agaacagcgc gaaggccatg ccggagtatt cgacgtggaa accgacgatt
     2341 tccgattcgc cttcggcgag g
//
LOCUS       DAOSDX010000451         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15709_length_2361_cov_3.341717, whole genome shotgun sequence.
ACCESSION   DAOSDX010000451 DAOSDX010000000
VERSION     DAOSDX010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15709_length_2361_cov_3.341717"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLN02_10020"
     CDS             complement(<1..234)
                     /locus_tag="PLN02_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOI52702.1"
                     /translation="MDCRIADEYRDTHAGQVAGEILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGQPVTAKTRLHLDRCLTC"
     gene            complement(234..1289)
                     /gene="glcE"
                     /locus_tag="PLN02_10025"
     CDS             complement(234..1289)
                     /gene="glcE"
                     /locus_tag="PLN02_10025"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HOI52703.1"
                     /translation="MTLDEIVAAIRAAHAGHTPLRLRGAGSKDFYGGLLAGEVLDLSG
                     HRGIVDYEPTELYVTAKCGTPLAEIEAALAEHGQMLAFEPPRFAGATLGGCIAAGLSG
                     PRRMQAGAVRDFVLGVKLVDGRGQVLNFGGQVMKNVAGYDVSRLIAGSLGTLGVIAEA
                     TLKVLPKPVAETTLEFVCAADEAVRRLNDWGAQPLPVSAAFWHEGRLWLRLSGAAAAV
                     DAAARKLGGKVVADAGRWWDEVREQRHAAFAAPVLWRVALPSTAPALAVDGLQAIEWG
                     GALRWYAGEIDAATIRAAASTASGHATLYRAPESLRCRVDPFTPLPPALLALHRRLKK
                     VFDPAGILNPGRLYAEF"
     gene            complement(1289..>2361)
                     /locus_tag="PLN02_10030"
     CDS             complement(1289..>2361)
                     /locus_tag="PLN02_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOI52704.1"
                     /translation="YAPDPSSQIACTLGGNVAENSGGVHCLKYGLTVHNVLRIRMVSI
                     EGEIFEIGSGAPDAPGYDLLALCIGSEGLLGVVTEVTVKLVPKPELAQVVMASFDDVA
                     QAGDAVAAIIAAGIIPAGLEMMDKPATAAVEPFVKAGYDLDAAAILLCESDGTPEEVA
                     EEIARVRAVLTGCGARDLRVSQSEAERLRFWAGRKAAFPAVGRITPDYYCMDGTIPRK
                     RLAEMLSAIAAMETKYGLSCANVFHAGDGNLHPLIMYDMSLPGDWEKAEAFGAEILEL
                     SVALGGSITGEHGVGIEKIKQMCVQYTTPELETFHAVKAAFDEKRLLNPEKAIPSLHR
                     CAEYGRMHVHRGELKFPDLERF"
BASE COUNT          365 a          826 c          807 g          363 t
ORIGIN      
        1 gcaggtcagg cagcggtcga ggtggagccg ggtcttggcg gtaaccggct gaccttcgag
       61 cacctgcttg atcaggtaga tgcggccgcg cggcccgtcg agttcgtcgc caaggagttg
      121 gtaagtcggg caggtggcgg tgcagaaacc gcaatgcacg cacttgcgca ggatttcgcc
      181 ggccacctgg ccggcgtgcg tgtcgcggta ttcgtcggcg atccggcagt ccatcagaac
      241 tccgcgtaaa gccggccggg gttgaggatg ccggccgggt cgaagacctt cttcagccgc
      301 cggtgcagcg cgagcagcgc cggcggcaac ggcgtgaacg gatcgacccg gcagcgcagc
      361 gattccggcg cccggtacag cgtcgcatgg ccgctggctg tactggcggc ggcgcggatc
      421 gttgccgcat caatctcgcc agcgtaccag cgcaaggcac cgccccattc gattgcctgc
      481 aggccgtcga ccgccagggc cggcgcggtg gatggcaggg cgacccgcca cagcaccggc
      541 gccgcgaaag cagcgtggcg ctgttcgcgc acttcgtccc accagcgccc ggcgtccgcc
      601 accaccttgc cgcccagctt gcgcgccgcc gcgtcgaccg ctgctgcggc cccggagagg
      661 cgcagccaaa gccgcccctc gtgccagaac gcggccgaca ccggcagcgg ctgcgcgccc
      721 cagtcgttca gccgacgcac cgcctcgtcg gcggcgcaga cgaattccag cgtcgtctcg
      781 gcaaccggct tcggcaacac cttcagcgtc gcctcggcga tcacgccgag cgtgcccagg
      841 ctgccggcga tcagccgcga aacgtcgtag ccggcgacgt tcttcatcac ctggccgcca
      901 aaattcagca cctgaccgcg cccgtcgacc aatttcacgc cgagcacgaa gtcgcgcacc
      961 gccccggcct gcatccggcg cggtccggac aggccagcgg cgatgcagcc gccgagcgtc
     1021 gccccggcga aacgcggcgg ctcgaaggcc agcatctgac catgttcggc cagcgccgcc
     1081 tcgatctcgg cgagcggcgt accgcatttc gcggtgacgt aaagttcggt cggctcgtaa
     1141 tcgacgatgc cgcgatgccc ggacagatcg agcacctcgc cggccagcag gccgccgtag
     1201 aaatccttgc tgccagcgcc gcgcaggcgc agcggcgtgt gcccggcgtg ggcggcgcgg
     1261 atggcggcga cgatttcgtc cagcgtcatc agaaacgctc caggtcgggg aatttcagtt
     1321 cgccgcgatg cacgtgcatg cgcccgtatt cggcgcagcg gtgcaggctg gggatcgcct
     1381 tctccgggtt gagcaggcgt ttctcgtcga acgccgcctt gaccgcgtgg aaggtttcca
     1441 gctccggcgt cgtgtactgc acgcacatct gcttgatctt ctcgatgccg acgccgtgct
     1501 cgccggtgat cgaaccgccg agcgccaccg acagctccag gatctcggcg ccgaaggcct
     1561 cggccttttc ccagtcaccg ggcagcgaca tgtcgtacat gatcagcggg tgcaggttgc
     1621 cgtcgccggc atggaagacg ttggcgcagc tcaggccgta tttcgtttcc atggcggcga
     1681 tcgccgacag catctcggcc aggcgtttgc gcggaatcgt gccgtccatg cagtagtagt
     1741 cgggcgtgat gcggccgacg gccggaaacg ccgccttgcg cccggcccag aagcgcaggc
     1801 gctcggcttc cgactgcgac acgcgcaggt cgcgggcacc acaaccggtg agcacggcgc
     1861 gcacgcgggc gatttcctcg gcgacttcct ccggcgtgcc gtccgactcg cagagcagga
     1921 tggcggcggc gtcgaggtcg tagccggcct tgacgaaggg ctcgacggcg gcggtcgccg
     1981 gcttgtccat catctccagc ccggccggga tgatgccggc ggcgatgatc gcggcgaccg
     2041 cgtcgccggc ctgggcgacg tcgtcgaatg aggccatcac cacctgggcg agttccggct
     2101 tcggcaccag cttcaccgtc acttcggtaa ccacgccgag caggccctcg gagccgatgc
     2161 acagggccaa caggtcgtag ccgggggcat ccggcgcgcc gctgccgatc tcgaagattt
     2221 cgccctcaat gctgaccatg cggatgcgca gcacgttgtg cacggtcagg ccgtatttca
     2281 ggcaatgcac gccgcccgag ttctcggcga cgttgccgcc gagcgtgcag gcgatctgcg
     2341 acgaggggtc gggcgcgtaa t
//
LOCUS       DAOSDX010000452         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15711_length_2361_cov_2.900694, whole genome shotgun sequence.
ACCESSION   DAOSDX010000452 DAOSDX010000000
VERSION     DAOSDX010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15711_length_2361_cov_2.900694"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..491)
                     /locus_tag="PLN02_10035"
     CDS             complement(81..491)
                     /locus_tag="PLN02_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HOI52705.1"
                     /translation="MKQGESMEAQQTTIYEKIGGAATVGRLADRFYELMDSVAQFKEL
                     RAMHPESLAGSRDKLYKFLSGWFGGPDLFVQEFGHPMLRARHMPFAIGTLERDQWVAC
                     MVLAMEDVGIDADVRKVLLNNFFNTADFMRNKEG"
     gene            complement(488..>2361)
                     /locus_tag="PLN02_10040"
     CDS             complement(488..>2361)
                     /locus_tag="PLN02_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOI52706.1"
                     /translation="DDAYTPNAEPNKRPDRAVTVYAQRCREAFAGTPVVIGSIEASLR
                     RIAHYDYWSDKVRRSVLPDSKADLLVFGNAERAIVELAHRLAKGEKIGEIRDLRGTAF
                     MVGRDWLPSADWDEMDSTEVDTPGALVKHADPYAMDERAGNSAGSALPATDGGQPVRI
                     VARAERLAARRERRAHSVVRLPAYEQVKDDPVLYAHASRTFHLESNPGNARALRQAHG
                     ERDVWLNPPPVPLSTEELDAVYELPYARRPHPAYGEAKIPAWEMIRFSVNIMRGCFGG
                     CTFCSITEHEGRIIQSRSEDSVIREIEEMRDKTPGFTGIVSDLGGPTANMYHLKCKDE
                     KIEAACRRLSCVYPDICDNLGTDHTPLISLYRRARSLKGVKKVLIGSGLRYDLAVRSP
                     EYIKELVQHHVGGYLKIAPEHTEEGPLSKMMKPGIGSYDRFKQLFDRFSREAGKEQYL
                     IPYFIAAHPGTTDADMVNLALWLKKNNFRLDQVQTFLPTPMALATTMYHSERNPLKKL
                     HRQVGETVGAVRGARQRKLHKAFLRYHDPENWPLLREALKEMGRADLIGNGKKQLVPA
                     WQPAGTGLKAAGAIRQTPERKGGAQRPTAATRNGTGGPRPATGGARQPGPRKTRSPR"
BASE COUNT          365 a          753 c          788 g          455 t
ORIGIN      
        1 caagggcgaa gcgtcgcagg cgctggacgc caacgacacg atctggaatt tcttcgtcga
       61 agtcgcggga agctgaaagc tcagccttcc ttgttgcgca tgaagtcggc ggtgttgaag
      121 aagttgttga gcagcacctt acgcacatcc gcgtcgatgc cgacgtcttc catcgccagc
      181 accatgcacg ccacccactg gtcgcgttcc agggtgccga tggcaaacgg catgtggcgg
      241 gcgcgcaaca tcggatggcc gaattcctgg acgaacaggt cggggccgcc gaaccagccg
      301 gagaggaact tgtacagctt gtcgcgcgaa ccggccaggc tttccgggtg catcgcgcgc
      361 aactccttga actgggcgac gctgtccatc aattcgtaga agcggtcggc gagccggccg
      421 accgtcgctg cgccaccgat tttctcgtag atggtggtct gttgtgcttc catgctttct
      481 ccctgcttca tcgtggtgat cttgtcttgc gcggccccgg ctggcgggcg ccgcccgttg
      541 ccgggcgagg tccgccggtg ccgttgcgcg ttgccgctgt cgggcgctgt gccccaccct
      601 ttctctctgg cgtctggcgg atggcgccag ccgccttcag cccggtaccg gccggctgcc
      661 aggccggcac cagttgtttc ttgccattgc cgatcaggtc ggcgcggccc atttccttca
      721 aggcttcgcg cagcaacggc cagttttccg ggtcgtggta gcgcaggaaa gccttgtgca
      781 gcttgcgctg gcgcgcgcca cgcacggcgc cgacggtttc tccgacctgg cggtgcagct
      841 tcttcagcgg atttctctcg ctgtgataca tcgtcgtcgc cagcgccatc ggcgtcggca
      901 ggaaggtctg cacctggtcg agacggaagt tgttcttctt cagccacagc gccaggttga
      961 ccatgtcggc gtcggtcgtc cccggatgcg cggcgatgaa gtagggaatc aggtactgct
     1021 ccttgcccgc ctcgcgcgaa aaacggtcga atagctgctt gaagcggtcg tagctgccga
     1081 tgccgggctt catcattttc gacagcgggc cttcttcggt atgttccggg gcgatcttca
     1141 ggtagccacc gacatggtgc tggaccagtt ccttgatgta ctcgggcgag cgcaccgcca
     1201 gatcgtagcg cagaccggag ccgatcagca ccttcttgac ccccttcagg ctgcgtgcgc
     1261 ggcggtagag cgaaatcagc ggcgtgtggt cggtgccgag gttgtcgcag atgtcgggat
     1321 agacgcagga cagccgcctg caggccgctt cgatcttctc gtccttgcac ttcaagtggt
     1381 acatgttggc ggtcggcccg ccgaggtcgg aaacgatgcc ggtgaagccc ggcgtcttgt
     1441 cgcgcatttc ctcgatctcg cggatcaccg aatcctcgga acggctctgg atgatgcgcc
     1501 cttcgtgctc ggtgatcgag cagaaggtgc aaccgccgaa acagccgcgc atgatgttga
     1561 ccgagaagcg gatcatctcc caggccggaa tcttcgcctc gccataggcc ggatgcggcc
     1621 gccgcgcgta aggtagctcg tagacggcgt cgagttcctc ggtggaaagc ggtaccggcg
     1681 gcgggttcaa ccagacgtca cgctcgccgt gcgcctggcg cagcgcgcgg gcgttgcccg
     1741 ggttggattc gaggtggaag gtgcgcgaag cgtgggcgta gagcaccgga tcgtccttga
     1801 cctgctcgta ggccggcaag cggacgacac tgtgggcgcg gcgctcacgg cgggcggcca
     1861 gacgctcggc acgcgcaacg atgcgcaccg gctggccgcc gtcggtggca ggcagcgccg
     1921 aaccggcact gttgccggcc ctctcgtcca tcgcgtaagg atcggcgtgt ttgaccagcg
     1981 cgccgggcgt atcgacctcg gtcgaatcca tttcgtccca gtcggccgac ggcagccagt
     2041 cgcgcccgac catgaaggcg gtaccgcgca gatcacggat ttcgccgatc ttctcgccct
     2101 tggccaggcg atgcgccagt tcgacgatgg cgcgctctgc attgccgaag accagcaggt
     2161 cggccttgga atcgggcagc accgaacggc gcaccttgtc cgaccagtag tcgtaatggg
     2221 cgatgcgccg caggctggcc tcgatcgagc cgatcaccac cggcgttccg gcgaaggcct
     2281 cgcggcagcg ctgggcgtac accgtgacgg cgcggtccgg ccgcttgttc ggctcggcat
     2341 tcggcgtgta ggcgtcgtcc g
//
LOCUS       DAOSDX010000453         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15724_length_2359_cov_3.994792, whole genome shotgun sequence.
ACCESSION   DAOSDX010000453 DAOSDX010000000
VERSION     DAOSDX010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15724_length_2359_cov_3.994792"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLN02_10045"
     CDS             complement(<1..515)
                     /locus_tag="PLN02_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOI52707.1"
                     /translation="MSSTAHLGERASGLSFLNPAAPEPWASFLEQIDRVAPHMGGLAK
                     WVETLKHPKRIMIVDVPIHRDDGTVAHYEGYRVQHNLSRGPGKGGVRFHPAVTLNEVM
                     ALSGWMTIKNAALNLPYGGAKGGIRLDPRAHSRDELERITRRYTSEIGVVIGPGKDIP
                     APDVGTNEQTMA"
     gene            complement(550..1278)
                     /locus_tag="PLN02_10050"
     CDS             complement(550..1278)
                     /locus_tag="PLN02_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOI52708.1"
                     /translation="MIQISHVSKSYGSFDVLKDCSTTVNKGEVIVVCGPSGSGKSTLI
                     KCVNGLEPFQSGEIIVNGTSVGDPKTNLSKLRAHVGMVFQNFELFPHMSITQNLAIAQ
                     VKVLGRAQDEAIDKGLKLLDRVGLKAHAHKFPGQLSGGQQQRVAIARALAMDPICMLF
                     DEPTSALDPEMVNEVLDVMTELAREGMTMMCVTHEMGFARRVANRVIFMDHGQIVEDD
                     EKEAFFANPRSERAQQFLAKILHH"
     gene            complement(1302..1985)
                     /gene="gltK"
                     /locus_tag="PLN02_10055"
     CDS             complement(1302..1985)
                     /gene="gltK"
                     /locus_tag="PLN02_10055"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HOI52709.1"
                     /translation="MVYEFDWSSIPGALPFLWDGMKISLEITLTAIVVGIVWGTLLAM
                     MRLSSVKPLSLFAAGYVNLFRAVPLVMVLLWFFLIVPKFIEQLFNLPPGADLRLTSAM
                     IGFALFESAYYSEIIRAGIQSVPKGQMAACQALGMTYGQAMRLVILPQAFRNMIPLLL
                     TQAIILFQDTSLVYVSALADFFGAAYKVGDRDGRLVELLLFAGLVYFVLCFAASMLVK
                     HLQKKYHPA"
     gene            complement(1987..>2359)
                     /locus_tag="PLN02_10060"
     CDS             complement(1987..>2359)
                     /locus_tag="PLN02_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI52710.1"
                     /translation="ARVAEQVRSGINALPRGQRNAGLALGLTLGQTYRFVMLPMAFRL
                     IVPPLTSEFLNIFKNSAVCSTIGLLELAAQGRQLVDYTAQPYESFIAVTVAYVAINII
                     VMTLMKKLEDKVRVPGYMGGK"
BASE COUNT          425 a          715 c          791 g          428 t
ORIGIN      
        1 gccatggtct gctcgttggt gccgacatcg ggcgccggaa tgtccttgcc cgggccgatg
       61 acgacaccga tttccgaggt gtaacggcgg gtgatgcgct ccagctcgtc acgcgaatgc
      121 gcccgcggat cgagccggat gccgcccttg gcgccgccgt agggcaggtt gagggcggcg
      181 ttcttgatcg tcatccagcc cgacagcgcc atcacttcgt tgagcgtcac cgccgggtgg
      241 aagcgcacgc cgcctttgcc cggcccccgc gacaggttgt gctggacgcg atagccctcg
      301 tagtgggcca cggtgccgtc gtcgcggtgg atcggcacat cgacgatcat gatgcgcttc
      361 gggtgcttca gggtttccac ccacttggcc aggccgccca tgtgcggggc gacacggtcg
      421 atctgctcga ggaagctggc ccagggctcg ggcgccgccg gattgaggaa ggaaagtccg
      481 ctcgcgcgct cgccgagatg tgctgtgctg ctcatgtctg tctccgtttt tggtgtgtca
      541 ggcaggtaat cagtggtgca ggatcttggc caggaactgc tgtgcccgtt ccgagcgcgg
      601 gttggcgaag aaggcttcct tctcgtcgtc ttcgacgatc tggccgtggt ccatgaagat
      661 gacccggttg gcgacgcggc gggcgaagcc catttcgtgc gtcacgcaca tcatggtcat
      721 gccttccctg gccagctcgg tcatcacgtc gagcacttca ttgaccattt ccgggtcgag
      781 cgccgaggtc ggctcgtcga acaacatgca gatcgggtcc atcgccagcg cccgggcgat
      841 cgccacccgc tgctgctggc caccggacaa ctggccggga aacttgtggg cgtgcgcctt
      901 caggccgacc cggtcgagca gcttgaggcc cttgtcgatg gcttcgtcct gtgcgcggcc
      961 gagcaccttg acctgggcga tcgccaggtt ctgggtgatg ctcatgtgcg ggaacagttc
     1021 gaagttctgg aagaccatcc cgacgtgggc gcgcagcttc gacaggttgg tcttcgggtc
     1081 gccgaccgag gtgccattga cgatgatctc gcccgactgg aagggttcga ggccattgac
     1141 acacttgatc agcgtcgatt tgccggaacc cgacgggccg cagacgacga tcacctcgcc
     1201 cttgttcacg gtggtgctgc agtccttgag cacgtcgaac gaaccgtagc tcttgctgac
     1261 atgggaaatc tggatcatga ctatctcctt ggagcacggc ctcaggccgg gtggtatttt
     1321 ttctgcaggt gcttgaccag catcgacgcg gcaaagcaga ggacgaaata gacgagaccg
     1381 gcgaacagca gcagttcgac caggcggccg tcgcggtcac cgaccttgta ggcggcgccg
     1441 aagaagtcgg ccagcgcgga cacgtaaacc agcgaggtgt cctggaacag gatgatggcc
     1501 tgggtcagca gcagcggaat catgttgcgg aaggcctgcg gcaggatcac caggcgcatc
     1561 gcctggccgt aggtcatgcc cagcgcctga caggccgcca tctggccctt cggcaccgac
     1621 tggatgccgg cgcggatgat ctccgagtag taggccgact cgaacagcgc gaagccgatc
     1681 atcgccgagg tcaggcgcag gtcggcgccc ggcggcaggt tgaacagctg ctcgatgaac
     1741 ttggggacga tcaggaagaa ccagagcagg accatgacca gcggcacggc gcggaacagg
     1801 ttcacgtagc cggcggcgaa cagcgacaag ggcttcaccg acgacagccg catcatcgcc
     1861 agcagcgtgc cccagacgat gccgacgacg atcgccgtca gcgtgatttc cagcgagatc
     1921 ttcatgccgt cccagaggaa gggcagcgcc ccggggatcg acgaccagtc gaattcgtag
     1981 accatgttac ttgcctccca tgtagcccgg cacgcggacc ttgtcttcga gcttcttcat
     2041 cagcgtcatg acgatgatgt tgatcgcgac ataagcgacg gtgacggcaa tgaaggattc
     2101 gtagggctgc gccgtgtaat cgaccaattg acggccttgc gcggcgagtt cgaggagacc
     2161 gatggtcgag cagacggcgg agttcttgaa gatgttgagg aattccgagg tcagcggcgg
     2221 cacgatcagc cggaaggcca tcggcaacat gacgaagcgg taggtctggc cgagggtgag
     2281 cccgagcgcc aggccggcgt tcctctggcc gcgcggcagc gcgttgatgc cggaacgcac
     2341 ttgctcggcc acccgcgcg
//
LOCUS       DAOSDX010000454         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15746_length_2357_cov_2.279757, whole genome shotgun sequence.
ACCESSION   DAOSDX010000454 DAOSDX010000000
VERSION     DAOSDX010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15746_length_2357_cov_2.279757"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLN02_10065"
     CDS             <1..735
                     /locus_tag="PLN02_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOI52711.1"
                     /translation="SLAGGIRSLMWSATHVRERLSLDHWHSLNSLQREQQAAQKKHPT
                     LTEAIAFLDRVLLVSASLNGFAMDNMTRDDGWRFLIVGRRLERLSFLALAIAHFLRMP
                     SSRAPGCLDWLLELADSIITYRSRYSRQAELLPVIDLLVFDESNPHGVLFQVEALGRY
                     VERMAEEVGHQPIERLQRAARRLQRFPLEPLERLQFSQCSCSAPCEDLAQLLDDLVAA
                     AAALSDALAMRYFTHVGDISRQTMAL"
     gene            737..1699
                     /locus_tag="PLN02_10070"
     CDS             737..1699
                     /locus_tag="PLN02_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOI52712.1"
                     /translation="MTAVRYHVLHETRYDYGSVVSLSQQQLHLSPRFLPWQEVFEQRL
                     DIEPAPTWRTDGEDAFGNPVTWISFHAPHDSLRIRSAMNVAVLPHPIDAETLAASLPW
                     ETVRECLAYDAHPPQPEDLDAVRFLFESPHVRVKHELADYAADCFTPGTPVLVGAQRL
                     MAKIFEEFEFDPEATTVSTPVLEVLENKRGVCQDFAHLMIACLRALGLAARYVSGYLL
                     TRPPPGKPRLIGADASHAWVSVYAPGTADNWVDFDPTNNLLPDTEHITLAIGRDFSDI
                     SPLRGIILGGGGAEPEVAVTVVPLDEEDIPEGLLDDSPVVADKP"
     gene            1699..>2357
                     /locus_tag="PLN02_10075"
     CDS             1699..>2357
                     /locus_tag="PLN02_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HOI52713.1"
                     /translation="MRRVAIVGGGPAGLMAAERLAGQSGLQVDLYDAMPSPGRKFLIA
                     GVGGMNITHAEALPAFIARYGARAAQIAPLLDAFGPAALREWIHGLGIETFVGSSGRV
                     FPREMKAAPLLRAWLHRLRGQGLRLHVRHRWLGWNAAGALRFATPDGEIALAADATLL
                     ALGGGSWAKLGSDGAWVPLLAAAGIPVAPLQPANCGFDVDWSTHFRERFAGAPVKSVI
                     AG"
BASE COUNT          330 a          867 c          777 g          383 t
ORIGIN      
        1 agcctggctg gcggcatccg cagcctgatg tggtcggcga cgcatgtccg cgagcgcctt
       61 tcgctcgatc actggcattc gctgaacagc ctgcagcgcg agcagcaggc ggcgcagaag
      121 aagcatccga cgctgaccga ggcgatcgcc ttcctcgacc gcgtgctgct ggtttcggcg
      181 tcgctcaacg gtttcgcgat ggacaacatg acgcgcgacg acggctggcg tttcctcatc
      241 gtcggccgcc gtctcgaacg cctcagcttc cttgcgctgg ccatcgccca cttcctgcgc
      301 atgccgtcca gccgggcgcc cggctgtctc gactggctgc tcgaactggc cgactcgatc
      361 atcacctacc gttcgcgtta ttcgcgccag gccgagctgt tgccggtgat cgacctgctg
      421 gtgtttgacg agagcaaccc gcacggcgtt ctcttccagg tcgaggcgct cggccgctac
      481 gtcgagcgga tggccgagga agtcggccac caaccgatcg aacgcctgca gcgcgccgcc
      541 cgccgccttc agcgtttccc gctggaaccg ctggagcgcc tgcagttcag ccagtgcagc
      601 tgcagcgcgc cgtgcgagga cctggcgcaa ctgctcgacg acctggtcgc cgccgccgcc
      661 gcgctctccg atgcgctggc catgcgctac ttcacccacg tcggcgacat cagccggcag
      721 accatggcgc tgtaacatga ccgccgtccg ctaccacgtc ctgcacgaga cccgttacga
      781 ctacggcagc gtggtctcgc tgtcgcagca gcaactgcac ctgtcgccgc gcttcctgcc
      841 ctggcaggaa gtgttcgagc agcgcctcga catcgagccg gcgccgacct ggcgcaccga
      901 cggcgaggac gccttcggca acccggtgac ctggatttcc ttccacgcgc cgcacgacag
      961 cctgcgcatc cgttcggcga tgaatgtcgc ggtgctgccg catccgatcg acgccgagac
     1021 gctggccgcc tcgctgccct gggaaacagt gcgcgagtgc ctggcctacg acgcccaccc
     1081 gccgcagccg gaagacctcg acgccgtccg tttcctgttc gaaagtccgc acgtccgggt
     1141 caagcacgaa ctggccgact acgcggccga ctgcttcact cccggcacgc cggtgctggt
     1201 cggggcgcag cggctgatgg cgaagatctt cgaggaattc gagttcgacc ccgaggcgac
     1261 cacggtgtcg acgccggtgc tcgaagtcct ggaaaacaag cgcggcgtct gccaggattt
     1321 cgcccacctg atgatcgcct gcctgcgtgc gctcggtctt gccgcccgct acgtcagcgg
     1381 ctacctgctg acccggccgc cgccgggcaa gccgcggctg atcggcgccg acgcctcgca
     1441 cgcctgggtc tcggtgtatg ccccggggac cgccgacaac tgggtcgatt tcgacccgac
     1501 caacaacctg ctgcccgata ccgagcacat cacgctggcg atcggccgcg atttctcgga
     1561 catctcgccc ctgcgcggca tcattctcgg cggcggcggc gccgaaccgg aagtggcggt
     1621 caccgtcgtg ccgctcgacg aagaggatat ccccgagggg ctcctcgacg actcgccggt
     1681 cgtcgccgac aagccctgat gcggcgcgtc gccatcgtcg gcggcgggcc ggccggcctg
     1741 atggccgccg agcgcctggc cggccagtcg ggactgcagg tcgacctcta cgacgcgatg
     1801 ccgtcgcccg gtcgcaagtt cctgatcgcc ggcgtcggcg gcatgaacat cacccatgcc
     1861 gaagcgctgc ctgccttcat cgcccgctac ggcgcgcgcg ccgcgcagat cgcgccgctg
     1921 ctcgacgcct tcggcccggc ggcgctgcgc gagtggatac atggcctggg catcgaaacc
     1981 ttcgtcggca gttcgggccg cgtcttcccc agggaaatga aggcggcgcc gctgctgcgc
     2041 gcctggctgc accgactgcg cgggcagggg ctgcggctgc atgtccggca ccgctggctg
     2101 ggctggaacg ccgctggcgc cctgcgcttc gcgacgccgg acggcgagat cgcgctggcc
     2161 gccgacgcca cgctgctggc gctgggcggc ggcagctggg cgaagctcgg ctcggacggc
     2221 gcctgggtgc cgctgctcgc tgccgccggc attccggtgg cgccgctgca gccggccaat
     2281 tgcggcttcg atgtcgactg gagcacgcat tttcgcgagc gcttcgccgg cgccccggtc
     2341 aagtcggtga tcgccgg
//
LOCUS       DAOSDX010000455         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15883_length_2340_cov_2.924289, whole genome shotgun sequence.
ACCESSION   DAOSDX010000455 DAOSDX010000000
VERSION     DAOSDX010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15883_length_2340_cov_2.924289"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..385)
                     /locus_tag="PLN02_10080"
     CDS             complement(14..385)
                     /locus_tag="PLN02_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52714.1"
                     /translation="MAKAIIGITDWTQTRSNLAALARRVDAGERLPEADYMLNFASPV
                     ALLEALPPKRLEALRTIRKVGPVSIYALAKKLCRNYSNIHSDVMRLLDLGLVEKDEHG
                     RICVPWDDVVVRVDGSLMVAA"
     gene            complement(387..674)
                     /locus_tag="PLN02_10085"
     CDS             complement(387..674)
                     /locus_tag="PLN02_10085"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HOI52715.1"
                     /translation="MRKATPIYRLNETRLDGVKVDMVIWQLPTPTPDRPHGLKYRLWA
                     GRDGKRLVRYDNESGKGDHKHLGEAGVEEVYCFVSLSKLVADFFADIEKGG"
     gene            complement(787..1964)
                     /locus_tag="PLN02_10090"
     CDS             complement(join(787..1712,1712..1964))
                     /locus_tag="PLN02_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611361.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOI52716.1"
                     /translation="MKIPKQEYTAEFKELAVKRVKDGQAVGAVAKDLGLIEQTLRNWV
                     KAHAAGKLTAPGARKITPEEMELSRLRAENIRLRRECEILKKSDGILRERCAVKYAWI
                     DTQRAHYALSEMCPVLDVSIGGYRAWKRGGKPERKRLTDAQLLALIRAIHAEFKGAYG
                     SPRMVLELRARGFSASKQRVERLMQENGIRARHKRKFKVTTDSKHGLPVADNLLDRNF
                     TPEAPNQVWTSDITYLWTDEGWLYLAIVLDLFNREVVGWSLKPRMTADIATDALTMAW
                     FRRKPAPGILHHSDRGSQYASQAFQGKLAEYGMTCSMSRKGNCWDNAPTESWFNSFKN
                     ERVHGVGFATREDMKATAFEYIEVFYNRKRLHSTLGYKSPTQFMHDWLTAQPTEKQVA
                     "
     gene            2004..>2340
                     /locus_tag="PLN02_10095"
     CDS             2004..>2340
                     /locus_tag="PLN02_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52717.1"
                     /translation="MENEISGEGHTPSKYVLREDRHGGLRRGEDGDGRTLTVEAYAQT
                     LFVLHGVASENGKQSTLRFLANDGLREALLRRWRGLAPMWLWGSQKPFSVQTTWNGEF
                     QERWTVASGK"
BASE COUNT          453 a          712 c          654 g          521 t
ORIGIN      
        1 ttcgatatcc gggctatgcg gcaaccatca gcgagccatc cacacgcacc accacgtcgt
       61 cccagggaac acatatccgg ccgtgctcgt ctttttccac aaggccgaga tcgagcaggc
      121 gcatgacgtc agaatggatg ttggaatagt tgcggcatag tttttttgcc aaggcataaa
      181 tcgaaacggg gccgactttc cgtatggttc gcagcgcttc aagcctcttg ggaggcaatg
      241 cttccagcag cgctaccgga ctggcaaaat tcaacatgta atccgcttca ggcaaccgtt
      301 cacccgcatc cacccgacgc gccagcgccg cgagattcga acgggtttgc gtccagtccg
      361 taattccaat aatcgccttg gccatatcag cccccttttt caatgtccgc aaagaagtcg
      421 gccaccagtt tcgatagcga cacgaagcag tacacttcct ctacaccggc ctcgccaaga
      481 tgcttgtggt cacccttgcc gctctcattg tcgtagcgca caagcctctt gccatcccgt
      541 ccggcccaca gccgatattt caagccatgc ggccggtccg gggtaggggt tggcaactgc
      601 cagatcacca tgtccacctt cacaccgtcg agtcgggttt cattcagtcg gtagattggc
      661 gtagctttcc gcatctattg cattctacgc aatatattga tcgtcgcaaa tccgggaaaa
      721 tggggtcaga cacgatatcg tgtatcctga ccttccctcg atttttcgtc ttccaacagg
      781 cggggttcat gctacctgtt tttccgtcgg ttgagccgtc agccaatcat gcatgaactg
      841 cgtcggcgac ttgtagccca gcgtcgagtg cagccgtttc cggttgtaaa acacctcgat
      901 gtattcgaac gccgtcgcct tcatgtcctc gcgcgtggca aatccaacgc catgcacccg
      961 ctcgttcttg aagctgttga accagctctc ggtcggggcg ttgtcccagc agttcccctt
     1021 gcgactcatc gagcaggtca tgccgtattc agccagcttg ccctggaacg cctggctggc
     1081 gtactggctg ccccggtcgg aatgatgcag gatgcccggc gcaggcttgc gccggaacca
     1141 ggccatggtc agcgcgtcgg tcgcgatatc cgccgtcatg cgtggcttca acgaccagcc
     1201 caccacttcg cggttgaaca gatcaaggac gatggccaga tacagccagc cttcgtccgt
     1261 ccacaggtag gtgatgtcgg aggtccagac ctggttcggc gcttcgggcg tgaagttccg
     1321 atccagcagg ttgtccgcca ccggcaggcc gtgcttcgag tcggtcgtga ccttgaattt
     1381 gcgcttgtgc cgcgcccgaa tgccgttctc ctgcatcagc cgctcgacgc gttgcttgct
     1441 cgcggagaat ccccgggcgc gcagttccag caccatgcgg gggctgccat aagcaccctt
     1501 gaactcggca tggatggcgc ggatcagcgc cagcagctga gcgtcggtca ggcgtttgcg
     1561 ttccggcttg ccgccgcgtt tccaggcacg gtagccgccg atgctgacgt ccagcacggg
     1621 gcacatctcg gacagggcgt agtgcgcgcg ttgcgtgtcg atccaggcat acttcacagc
     1681 gcatctttcg cgaagtatgc cgtcgctttt tttaggattt cgcactcccg gcgcagccgg
     1741 atgttctcgg cacgtagccg ggacagttcc atttcttccg gggtgatctt ccgggcacca
     1801 ggagcggtga gcttgcctgc ggcgtgcgct ttgacccagt tacgcagggt ctgctcgatc
     1861 aatcccaaat ccttggccac cgcgcccacc gcctggccgt ccttgacacg cttgaccgcc
     1921 agttccttga attcggcggt gtattcctgc ttcggtatct tcatctttcc tcttcccaag
     1981 ttaacgccaa tactacgtca cccttggaaa acgaaatttc gggggaaggt cacacaccaa
     2041 gcaaatatgt attaagggag gatcgtcatg gaggattacg acgaggggaa gacggtgatg
     2101 ggcggacgct taccgtcgag gcttacgccc agacgctgtt cgtgctgcat ggggtcgcct
     2161 cagaaaacgg aaaacaaagc acgctaaggt ttctcgcaaa cgacggtctg cgagaagcct
     2221 tgctgagaag gtggcgcggt ctagcgccga tgtggctgtg gggttctcaa aaaccgttct
     2281 cggttcaaac gacctggaac ggcgagtttc aagaacgctg gacagttgcc agcggcaaga
//
LOCUS       DAOSDX010000456         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15896_length_2339_cov_2.306480, whole genome shotgun sequence.
ACCESSION   DAOSDX010000456 DAOSDX010000000
VERSION     DAOSDX010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15896_length_2339_cov_2.306480"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1249)
                     /gene="cysS"
                     /locus_tag="PLN02_10100"
                     /pseudo
     CDS             complement(65..1249)
                     /gene="cysS"
                     /locus_tag="PLN02_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
     gene            1352..>2339
                     /locus_tag="PLN02_10105"
     CDS             1352..>2339
                     /locus_tag="PLN02_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI52718.1"
                     /translation="MTTLSLFQPRFRTLRAFAFCLSIGFAGPVLADNLPDIQRLIKQG
                     QHAQALEKVDQYLSSRPKDAQGRFLKGLILTELNRPADAIGVFTKLSEDYPELPEPYN
                     NLAVLYAQQKQYDKARTALEMAIRTHPSYAIAYENLGDVYAKLASQAYDKALQLDGAN
                     ATTQNKLALIRDLISTSGKGNVRPPVVAAAPAPAPVPAPAPAPVAAAKPTPTATVVAT
                     TPGAAAAAPTPVAAAETKRPAPAKAAGNDDVAQAIASWAQAWSQKDMKAYFAAYANSF
                     EPGKGASRKAWEAERSQRIGERPGKISVTYEEPTISVDGNTATARFRQHYKAP"
BASE COUNT          494 a          799 c          654 g          392 t
ORIGIN      
        1 tggcaatcaa cgcctcgacc gccgcctcat caatcccgtc ggccgacccg cctgaccgca
       61 attaggcctg cggctcgcgt tcaagcaggc cgatcaccgc cgccagcgcc ttgagcagac
      121 cagagagctc gccggatttc tggcgattgg cttccgccgc cagttcgaaa agcacggcaa
      181 tcgcaccgtg cgaatcgaaa tcctcgttca aggcagcggc aaaacgggcg gcaaaatcct
      241 gcttccagtc gatggccacc ggcaccggcg ccacgtcgcg caacgtgaag taaagaccgt
      301 cgagcgagcg cttggcgtcg tcgaggtggg catcggaata attcagcgga ctgcggtaat
      361 gggcacgcag gatgaagaag cgaacgactt cggcatcgaa tttcttcagc acctcgcgga
      421 tggtgaagaa attgcccagc gacttcgaca tcttttcgtt gtcgacccgg acaaaaccgt
      481 tgtgcatcca gtaattgacg tagccgcagc aattcgcgcc ctcggactgg gcgatctcgt
      541 tctcgtgatg cggaaactgc agatcctgac cgccgccgtg gatgtcgaaa tgcttgccga
      601 gcagcgacga actcatcgcc gagcattcga tatgccagcc cggccgtcct tcgccccagg
      661 gcgacgccca ggccggctcg cccggcttgg catgcttcca gagaacgaaa tcgagcgggt
      721 cctgcttggc ggtatcgact tcgacgcgct caccggcgcg caggtcgtcg agcgatttgc
      781 cggacagctt gccgtagccc ggaaacttgc gcaccgcata attgacgtcg ccgtcggccg
      841 ccagataagc caggccattg gcttcgagct taccgatcag ctccagcatc tgcggcacat
      901 actcggtcgc ccgcggctcg tgatcggggc gcagcacgcc cagcgcgtcg gcgtcctcgt
      961 gcatgaaacc gatgaaacga tcggtcaatt gctggatgct ttcaccgttc tccgccgcac
     1021 gacgaatgat cttgtcatcg atatcggtaa tattgcgaac gtaggtcacc tcgtagccac
     1081 gcgttctcag ccagcgataa accatgtcga agacgaccat cacccgggca tggcccaggt
     1141 gacaatagtc gtagaccgtc atcccgcaga catacatgcg aaccttgttg gcttcgatcg
     1201 gttcgaaaac ctgcttttcg cgcttgaggg agttgaatat ctttagcatg acggaatgaa
     1261 aagatgcaga attggccctg caaacctatg gctttatccg cttctttggt agaatcgagt
     1321 gattgaaaaa ctcagaaatt atagcacggc gatgaccacc ctatccttgt tccagcctcg
     1381 cttccggacg ctacgcgcgt tcgccttctg cctgtcaatc ggctttgccg gcccggtact
     1441 ggccgacaac ctgcctgaca tccagcgctt gatcaaacag ggacagcacg cacaggccct
     1501 ggaaaaggtc gaccaatatc tgagcagccg gcccaaagat gcccaggggc gtttcctcaa
     1561 ggggctgatc ctcaccgaac tgaaccgccc cgccgatgcc atcggcgtct tcaccaagct
     1621 ctccgaggac tatccggagc tgcccgaacc ctacaacaac ctggccgtgc tctacgccca
     1681 gcagaagcag tatgacaaag cgcgcaccgc cctggaaatg gcgatccgca ctcacccctc
     1741 ctacgccatc gcctacgaga acctcggcga cgtctatgcc aagctggcca gccaggccta
     1801 cgacaaggcg ctgcaactcg acggcgcgaa tgcgaccacg cagaacaagc tggcgttgat
     1861 ccgcgacctg atcagcacct ccggcaaggg caacgtccgg ccgccagtgg tcgccgccgc
     1921 tccggcaccg gcgcccgttc cggccccagc gcccgccccg gtcgccgccg ccaaaccgac
     1981 gccgactgcc accgtcgtcg ccaccacgcc gggcgccgcc gccgcggccc ccaccccggt
     2041 cgccgccgcc gaaaccaagc gaccggcccc ggccaaggca gccggcaacg acgacgtggc
     2101 gcaggccatc gcctcctggg cccaagcctg gtcgcaaaag gacatgaagg cctactttgc
     2161 cgcctacgcc aacagcttcg aacccgggaa gggcgccagc cgcaaggcct gggaagctga
     2221 acgctcgcag cggatcggcg aacgccccgg caagatttcg gtaacttacg aggaaccgac
     2281 gatcagcgtg gacggcaaca ctgccacggc ccgtttccgc cagcactaca aggcaccgg
//
LOCUS       DAOSDX010000457         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15900_length_2338_cov_2.496715, whole genome shotgun sequence.
ACCESSION   DAOSDX010000457 DAOSDX010000000
VERSION     DAOSDX010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15900_length_2338_cov_2.496715"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2231
                     /locus_tag="PLN02_10110"
     CDS             <1..2231
                     /locus_tag="PLN02_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52719.1"
                     /translation="RNVGRMLASESLPISSLSQESLIADLVGAPYLEGLAVGGNGRVI
                     VATDSALLGLAAASLPGIDPRWLAAEAPDEQWIAAGDRLTSVQRLRGESASAPLYTAI
                     IAVSTEALSARKQSIILLGVIGSLFFIVLSSAVLIVVAQRFIGRRVSATLAVLKRVEE
                     GALDERIPVTSEDELGELQRGTNSMIARVREILDELRASREALARSEERMALALQGAN
                     DGIWDWDLVSNEVYFSPRWKSMLGYGEHELADEFATWERLTDDEGRERTMRLIDDVLS
                     GRARVFDIEFRMRHKAGDWVDIRSRAFLQRDADGKPLRLVGTHVDISERKRAEAELDA
                     HRHHLESLVKARTAELEVARDIAEAASRAKSTFLANMSHEIRTPMNAIMGMTAIALRH
                     AEDARLRDQLEKIDSASRHLLHVINDILDISKIEAERLILERTRFRLGEPVENLLSLI
                     GHKVTEKGLRLDVDLAPDLAACHVLGDPVRLGQVLINLTANAIKFTQQGSIVLRCRVL
                     EENRNGLQLLWEVVDTGIGIAAEDQKRLFNAFEQTDSSMTRKYGGTGLGLAISRRLVK
                     MMGGEIGVESALARGSRFWFTVRLEKAGDVAVEAAPSDEALSAEARLLQDYAGVRVLL
                     AEDEPINQEVSLGLLGDVGLTVDLAEDGEKAVALASEKRYDLILMDMQMPNLNGLDAT
                     RAIRAGGPNAATPILAMTANAFDEDRQVCLNAGMNDHIAKPIDPDHLYMTLLHWLAKR
                     AA"
BASE COUNT          411 a          792 c          789 g          346 t
ORIGIN      
        1 tgcgcaacgt cggccgcatg ctggcgagcg agagcctgcc gatcagctcg ttgtcgcagg
       61 aaagtctgat cgccgacctg gttggcgccc cctatctcga aggcctggcc gtcggcggca
      121 acggccgcgt catcgtggcc accgacagcg ccttgctcgg cctggcagcc gcatcgctgc
      181 cggggatcga cccgcgctgg ctggccgccg aagcgccgga cgaacaatgg atcgccgccg
      241 gcgaccgcct gaccagcgtc cagcgcctgc gcggggagtc cgccagcgcg ccgctgtaca
      301 cggcgatcat cgccgtcagt accgaggcgc tatcggcgcg caagcagtcg atcatcctgc
      361 tcggcgtgat cggttcgctg ttcttcatcg tcctcagttc ggccgtgctg atcgtcgtcg
      421 cccagcgttt catcggccgc cgggtcagcg cgacgctggc cgtcctcaag cgcgtcgagg
      481 agggggcgct cgacgagcgc attccagtca ccagcgagga cgaactgggc gaactgcagc
      541 gcggcaccaa ctcgatgatc gcccgggtgc gggagatcct cgacgaactc cgggcctcgc
      601 gcgaagcgct ggcgcgcagc gaagaacgca tggcgctggc cctgcagggc gccaacgacg
      661 gcatctggga ttgggacctg gtcagcaacg aggtctattt ctcgccgcgc tggaagtcga
      721 tgctgggcta cggcgagcac gaactggcgg atgaattcgc cacctgggaa aggctgaccg
      781 acgacgaggg ccgggaacgc acgatgcgcc tgatcgacga cgtgctgagc ggccgggcca
      841 gggtcttcga catcgaattc cgcatgcgcc acaaggccgg cgattgggtg gacatccgtt
      901 cccgcgcctt tctccagcgc gacgccgacg gcaagccgct gcgcctggtc ggcacgcacg
      961 tcgacatcag cgagcgcaag cgggcggaag ccgagctcga cgcccaccgc catcacctgg
     1021 aatcgctggt caaggcgcgc accgccgaac tcgaagtggc cagggacatc gccgaggcgg
     1081 caagccgggc caagagcacc ttcctcgcca acatgagcca cgagatccgc acgccgatga
     1141 acgccatcat gggcatgacg gcgatcgccc tgcgccacgc cgaggacgcc aggctgcgcg
     1201 accagctgga aaagatcgac tcggcgtcgc gccatctgct gcacgtcatc aacgacatcc
     1261 tcgacatctc gaagatcgaa gccgagcgcc tgatcctcga gcgcacccgg ttccgcctcg
     1321 gcgagccggt ggaaaacctg ttgagcctga tcggccacaa ggtgacggaa aaggggctgc
     1381 gtctcgatgt cgatctggcg ccggatctcg ccgcttgcca tgtactcggc gacccggtgc
     1441 gcctcggtca ggtgctgatc aatctgaccg ccaacgcgat caagttcacc cagcagggtt
     1501 cgatcgtcct gcgctgccgg gttctggaag aaaatcgcaa tggactgcag ttgctctggg
     1561 aggtcgtcga taccggcatc ggtatcgcgg ccgaggacca gaagcgcttg ttcaatgctt
     1621 tcgagcagac cgacagctcg atgacccgca aatacggcgg caccggcctc gggctggcga
     1681 tcagccggcg cctggtgaag atgatgggcg gcgaaatcgg cgtcgaaagc gcgcttgccc
     1741 ggggcagccg cttctggttc acggtccgcc tggaaaaggc cggcgacgtg gcggtcgagg
     1801 cggcgccgag cgacgaggcg ctctccgccg aagcccgcct gctgcaggac tacgccggcg
     1861 tccgcgtgct gctggccgag gacgagccga tcaaccagga agtgtcgctc ggcctgctcg
     1921 gcgacgtcgg cctgacggtc gacctggcgg aagacgggga gaaggcggtg gcgctggcca
     1981 gcgagaaacg ctacgacctg atcctgatgg acatgcagat gccgaacctg aacggcctcg
     2041 atgcgacgcg ggcgatccgc gccggcggcc cgaacgcggc gacgccgatc ctggcgatga
     2101 ccgccaacgc cttcgacgag gatcgccagg tctgtctcaa tgccggcatg aacgaccaca
     2161 tcgccaagcc gatcgacccc gatcacctct acatgacgct gctgcactgg ctggcgaagc
     2221 gcgccgcctg aagcgggacg ccccggcgcc ggccggctgc ctccctgttg cgtcaggcgc
     2281 tggcggccgg cagcgtcagc agcaccgccg gcaggatcgc cgtttcctcg cagttgtt
//
LOCUS       DAOSDX010000458         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15929_length_2335_cov_3.157895, whole genome shotgun sequence.
ACCESSION   DAOSDX010000458 DAOSDX010000000
VERSION     DAOSDX010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15929_length_2335_cov_3.157895"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLN02_10115"
     CDS             <1..307
                     /locus_tag="PLN02_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOI52720.1"
                     /translation="TAGQWVIKAFLLNILNPKLSIFFLAFLPQFVDPAQGSPLLQLAQ
                     LSAVFMLMTFVVFVVYGLLAHAFRRTVIEAPRVQSWLRRGFAGVFAGLGVHLAQAER"
     gene            307..1005
                     /locus_tag="PLN02_10120"
     CDS             307..1005
                     /locus_tag="PLN02_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOI52721.1"
                     /translation="MTMKTLGLIGGMSWESTLPYYRLINEQVRARLGGLHSAKLVLYS
                     VDFAEIEQLQQSGEWVTAGARLADAARALAAAGAEALVLCTNTMHKVAPAIAAAVNLP
                     LLHIADPTAAAIRAAGLTRVGLLGTRFTMEQDFYVERLRAHGLEVLLPNAEQREIVHS
                     VIYDELCLGRSEPTSRAAYRAIIADLACRGAEAVILGCTEISLLIGPDDSPLPLFDTT
                     RLHAQAAVDFALAD"
     gene            complement(1014..2036)
                     /locus_tag="PLN02_10125"
     CDS             complement(1014..2036)
                     /locus_tag="PLN02_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOI52722.1"
                     /translation="MPSPAKPDICRPTGDAELAMLDVYMPMMKSAAIVSAGQLGLFEA
                     LAAGPLAASELAGRLQASARGIATLADFLVAIGYLERQDEAYANTASTQRWFTSRGTV
                     DYTPGLLWTAEAWPMLGELAGAVRRGGPQTTLWQAMQARPQLGERFSAYMGAFARDLG
                     PDLLARVPVGDDQRRLLDLGGSHGLHSIRFCERYPQLSAQIVDLPSALSETPATIAAH
                     GLAERIALTPGDVLEFDWGQGFDLVFYLSVAHNQSADDNRRVIRRIRDSLKPGGLLVI
                     HEYLDDQPPGAYHAAFKLTLLYETGTQIHREADYRDWLAEAGFASVERIDLDPREKGS
                     LLLARC"
     gene            complement(2036..>2335)
                     /locus_tag="PLN02_10130"
     CDS             complement(2036..>2335)
                     /locus_tag="PLN02_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOI52723.1"
                     /translation="CSSNYGVSFPLFAKLQINSAPRHPLYAALIAAAPATVFPAGSDF
                     RAKLAGYNMLPANDSDVLWNFEKFLVDRDGRVVGRFSPDTAPDDAALRVAIEAGL"
BASE COUNT          358 a          775 c          812 g          390 t
ORIGIN      
        1 gacggccggc cagtgggtca tcaaggcctt cctgctcaac atcctgaatc ccaagctgag
       61 cattttcttc ctcgccttcc tgccgcagtt cgtcgatccc gcccagggtt cgcccttgtt
      121 gcaactggcg cagctcagcg ccgtcttcat gttgatgacc ttcgtcgttt tcgttgtcta
      181 cgggctgctg gcgcacgcct tccggcgcac ggtcatcgag gcaccgcgcg tccagtcctg
      241 gttacggcgc ggctttgccg gcgtttttgc cggcctcggc gtccatctgg cccaggcgga
      301 gcgctgatga cgatgaagac cctcggcctg atcggcggca tgagctggga atcgacgctg
      361 ccctattacc gcctgatcaa cgaacaggtc cgcgcccgcc tgggcggcct gcattcggcg
      421 aagctggtgc tctacagcgt cgacttcgcc gagatcgagc agttgcagca gtccggcgaa
      481 tgggtcacgg ccggcgcccg cctggccgac gccgcccgcg ccctggcggc ggccggcgcc
      541 gaagcgctgg tgttgtgcac caacaccatg cacaaggtag cgccagccat tgcggcggcg
      601 gtaaacctgc cgctgctgca catcgccgac ccgacggcgg cggcgatccg ggctgccggg
      661 ctgacgcggg tcggcttgct tggcacgcgc ttcacgatgg aacaggattt ctacgtcgag
      721 cgcctgcgcg cgcacgggct ggaggtgctg ctgcccaatg ccgagcagcg cgagatcgtg
      781 catagtgtga tctacgacga gttgtgcctt ggccgcagcg aaccgacgtc gcgcgctgct
      841 taccgcgcca tcatcgccga cctggcctgc cgcggcgccg aagcggtgat acttggctgc
      901 acggaaattt cgctgctgat cggcccggac gactcgccgc tgcccttgtt cgacaccacg
      961 cggctgcatg cgcaggcggc ggtggatttc gcgctggccg actgaaacca gcctcagcag
     1021 cgggccagca gcaacgagcc cttttcccgt ggatcaaggt cgatgcgttc gacgctggca
     1081 aagcccgctt cggccagcca gtcgcgatag tcggcttcgc ggtggatctg cgtcccggtc
     1141 tcgtagagca acgtcagttt gaaggcggcg tggtaggcgc cgggcggctg gtcgtcgagg
     1201 tattcgtgga tcaccagcag gccgccgggc ttcaggctat cgcggatgcg ccggatgacg
     1261 cggcggttgt cgtcggcgct ctggttgtgc gcgaccgaga ggtagaagac gaggtcgaag
     1321 ccctgccccc agtcgaactc gaggacatca cccggggtca acgcgatgcg ctcggccagg
     1381 ccgtgggcgg cgatggtcgc cggcgtttcg ctcagcgcgc tgggcaggtc gacgatctgc
     1441 gcgctgagtt gcggatagcg ctcgcagaag cggatcgaat gcaagccgtg cgagccgccg
     1501 aggtcgagca ggcggcgctg gtcgtcgcct accggcacac gcgccagcaa gtcggggccg
     1561 aggtcgcggg cgaaggcgcc catgtaggcg gaaaaccgct cgccgagctg cggccgcgcc
     1621 tgcatcgcct gccacagcgt ggtctgcggc ccgccgcggc gcaccgcacc ggccagctcg
     1681 cccagcatcg gccaggcttc ggcggtccac agcaggcccg gcgtgtaatc gacagtgccg
     1741 cgtgaggtga accagcgctg ggtcgacgcc gtgttggcgt aggcttcgtc ctggcgttcg
     1801 agatagccga tggcgacgag gaaatcggcc agcgtcgcga tgccgcgcgc cgacgcctgc
     1861 agccgcccgg ccaattcgct ggcggccagc ggcccggcgg cgagcgcttc gaacaggccg
     1921 agctggccgg ccgagacgat ggccgccgat ttcatcatcg gcatatagac gtcgagcatc
     1981 gccagctcgg cgtcgccggt cgggcgacag atatcgggct tggccggcga cggcatcaga
     2041 gccctgcctc gattgccacg cgcagcgccg cgtcgtcggg cgcggtatcg ggcgagaagc
     2101 ggccgacgac gcgaccgtcg cggtcaacca ggaatttctc gaaattccac agcacgtcgc
     2161 tgtcattggc cggcagcatg ttgtagccag cgagcttggc gcggaaatcg ctgccagccg
     2221 ggaagacggt cgccggtgcc gccgcgatca gcgccgcgta gagcggatgg cgcggcgcgc
     2281 tgttgatctg caacttggcg aacagcggaa agctgacgcc gtaattgctg ctgca
//
LOCUS       DAOSDX010000459         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15967_length_2331_cov_3.844025, whole genome shotgun sequence.
ACCESSION   DAOSDX010000459 DAOSDX010000000
VERSION     DAOSDX010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_2331_cov_3.844025"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="PLN02_10135"
     CDS             complement(<1..1200)
                     /locus_tag="PLN02_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52724.1"
                     /translation="MSGLAKLRVTTRMHILSGLMLVGLLVLCLISLFEIREGMLHDRQ
                     DKTRDLVEVALGIVKHQHQLASSGALTEEQAKQAARDSLRGLRYGENDYFFGFDTEGV
                     YFLHGGNPQMEGQKKIDMQDTNGKYIIRELVAAARAGGGFVDYWFPRAGQKEAEPKLG
                     YAVLFPQWNWVIGTGIYIDDVDQAFNEIAWLLGGISAVLLAVLLFTGLLVSRSVIRQL
                     GGEPADAAAIMERVASGDLSTNIAQAPAGSLLHALSGMVGALRQMVAEINNDASRLVS
                     NAEQISSASDQVAAAAEQQSDATSSMAAAIEELTVSSNHISDSARDTSDDSTSAVELS
                     GQGSQRVDKAAAAIRQIADTVSDASTRIHALEERARQISDIANVIKDIAGQTNLLALN
                     AAIEAARA"
     gene            complement(1320..1808)
                     /locus_tag="PLN02_10140"
     CDS             complement(1320..1808)
                     /locus_tag="PLN02_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOI52725.1"
                     /translation="MSLLIHAAPFLAGNWLADAPPPTPRPPPLQAQLQAPPPVPELIV
                     PETPKPPAEKSATRPRQAAPSGPGATASTWTRAIREQFSRQQQAGLFYPAEAIRLGLE
                     GEALVFMMLDADGNVVAARVEAGSGHELLDRAALDAVRRLRALPADAPQEALLPVRFR
                     LH"
     gene            1921..>2331
                     /locus_tag="PLN02_10145"
     CDS             1921..>2331
                     /locus_tag="PLN02_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOI52726.1"
                     /translation="MIIGMVLVCPARAQTWLGGEVRLPDPVSFANRMEMGDVAQAKAW
                     LDAGMAPDFMGSRIGSGLMIAAWEGNIPLMRLFLSRGANLGLSNANGETALALAAWRG
                     QLEAVQWLVERGAPVNAPPRQWSPLHYAVFGGHAA"
BASE COUNT          363 a          700 c          834 g          434 t
ORIGIN      
        1 ggcgcgcgct gcctcgattg ccgcgttgag cgccagcagg ttggtctggc cggcgatgtc
       61 cttgatcacg ttggcgatgt cggaaatctg ccgcgcacgc tcttccagcg cgtggatgcg
      121 ggtcgaggcg tcggagacgg tatcggcgat ctggcggatc gccgccgccg ccttgtccac
      181 gcgttgcgaa ccctggccgg agagttcgac ggcgctggtc gaatcgtcgg aggtgtcgcg
      241 ggcgctgtcg gaaatgtggt tggaactgac cgtcagttcc tcgattgccg ccgccatcga
      301 cgaagtcgca tcggattgct gctcggccgc ggcggcgacc tgatcggagg cgctactgat
      361 ctgttcggcg ttgctgacca gccggctggc gtcgttgttg atctcggcga ccatctggcg
      421 cagtgcgccg accatgccgc tgagcgcatg cagcaggctg ccggccggcg cctgagcgat
      481 gttggtgctg aggtcgccgc tggcaacgcg ttccatgatt gccgccgcgt cggcaggttc
      541 gccgccgagc tgccggatca cgctgcggct gaccagcagg ccggtgaaca gcagaacggc
      601 cagcagtact gcggaaatgc cgccgagcaa ccaggcgatt tcgttgaatg cctggtcgac
      661 gtcgtcgatg tagatgccgg tgccgatcac ccagttccac tgcgggaaca gtaccgcata
      721 gccgagtttc ggttcggctt ccttctggcc ggcgcgcgga aaccagtaat cgacgaagcc
      781 gccgccggct ctggcggcgg cgaccagttc gcggatgatg tacttgccgt tggtgtcctg
      841 catgtcgatc ttcttctggc cttccatctg cgggttgcca ccatgcagga aatagacgcc
      901 ttcggtatcg aagccgaaga agtaatcgtt ctcgccgtag cgtagcccgc gcaggctgtc
      961 gcgcgccgcc tgcttggcct gttcctcggt cagcgccccg ctgctcgcca gctggtgctg
     1021 atgcttgacg atgccgaggg ccacctcgac caggtcgcgg gtcttgtcct ggcggtcatg
     1081 cagcatgccc tcgcgaatct cgaacagcga aatcaggcag aggacgagca ggccgaccaa
     1141 catcaggcca ctgaggatgt gcatgcgcgt ggtgacgcgc agttttgcca agccgctcat
     1201 ttttagctcc ctgtagcaag tggttctgtt tatggttaac gagcatagtc tataaccgaa
     1261 ccctgttttt gatacaggtc aaagcttgca cattggtgaa ttttgttaat tgtgttgttt
     1321 tagtgaagcc ggaagcgtac cggcagcagc gcttcctgcg gcgcgtcggc gggcagggcg
     1381 cgcaggcggc gtaccgcgtc cagtgccgcc cggtcgagca gttcgtggcc gctgccggct
     1441 tcaacgcggg cggcgacgac gttgccgtcg gcgtccagca tcatgaacac cagcgcctcg
     1501 ccttcgaggc cgaggcggat cgcttcggcc gggtagaaca ggccggcctg ctgctggcgc
     1561 gagaattgtt cgcggatcgc ccgcgtccag gtgctggctg tcgcgccggg tccgctcggc
     1621 gcagcctgcc gggggcgggt ggcggatttt tcggctggcg gcttcggtgt ctcgggaacg
     1681 atcagttccg gcaccggcgg cggagcctgc aactgcgcct gcagcggtgg cgggcgcggg
     1741 gtcggcgggg gcgcgtcggc cagccagttc ccggcgagaa agggagcggc gtgaatgagc
     1801 agcgacacac agaaagcagg cagcaggaaa cgatgcgtcc ggagcatgag agaatggcgc
     1861 ctgttcgcag cgcaaacggg aagtcggatg aagggaatgg caggcaggct ggtagcggca
     1921 atgataatcg ggatggtgct ggtttgcccg gcccgggccc agacctggtt gggcggcgag
     1981 gtccgcctgc ccgatccggt gagcttcgcc aaccggatgg aaatgggcga cgtggcgcag
     2041 gccaaggcct ggctggacgc gggcatggcg ccggatttca tgggcagccg gatcggttcc
     2101 gggctgatga tcgccgcctg ggagggcaat attccgctga tgcgcctgtt cctgtcgcgc
     2161 ggcgccaatc tggggctgag caacgccaac ggcgaaacgg cgctggcgct ggccgcctgg
     2221 cgcggccaac tcgaagcggt gcagtggctg gtcgaacgcg gggcgccggt caatgcgcca
     2281 ccgcgccagt ggtcgccgct gcattacgcg gtgttcggcg gtcacgcggc g
//
LOCUS       DAOSDX010000460         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15983_length_2329_cov_4.581354, whole genome shotgun sequence.
ACCESSION   DAOSDX010000460 DAOSDX010000000
VERSION     DAOSDX010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15983_length_2329_cov_4.581354"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..730)
                     /locus_tag="PLN02_10150"
     CDS             complement(80..730)
                     /locus_tag="PLN02_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HOI52727.1"
                     /translation="MSIDEHLLRLLVSFFLGGLISLATITNPLSKIPLFLSLAAGRAA
                     PEATLEARRACFYGFLLLAGGLFAGVMILEAFGISFGALRIAGGLTVSIIGYRMLFGT
                     SDTTLTGSGGSFAFFPLAMPGIAGPGALATVIGISSEIAELDGGISRLLAYTATVASI
                     AATCLLIWLVLRSARWVSRRLGSEGINVVARLNGFLLVCIGVQFVGSGIRSFVAGT"
     gene            complement(733..>2329)
                     /locus_tag="PLN02_10155"
     CDS             complement(733..>2329)
                     /locus_tag="PLN02_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI52728.1"
                     /translation="AFTVVVGLFVIVAGWTTFSLGRVDAAAERIASVSLTKDSQIAEL
                     GYAYAGMRESVRNNIIFTDAEVMKAEAARYEREKARFFAALGALEKIAVDHAVVGEAA
                     LLQRVRQQATAAIDIQDKAMAEAMQFLSTVAMGILQQEAEPRRQVLEASIAEARQLVQ
                     AQSRERAGDIVAESANTRRLTLILVVVAVLIAAVLGYLLTGGVRRPLETTARLMESIA
                     AGDLTQRIEPNGFAEIRRVQAAAMKTTQSLAGILGAIRAEAGQLKHAVTALSGSADEM
                     RGGSEAQAAASAAMAETLQTMSVCITHIANLGQEAQDLSGKAGQQAAGGCQTIQTMVD
                     EIRQIAGLVTDSAATATTLGQESERISAITSVIHDLADQTNLLALNAAIEAARAGDSG
                     RGFAVVADEVRKLAEKTTASASQIAAMVSAIQNGARSMAEHMQRSVSRVDEGLRMACA
                     AGESMSAIESSNRHVAQVIDDVSASLRQQSSGGEEIAARVEHIVQMIHENNRATASMA
                     TTARQLDNLAGSLESGIARFRTA"
BASE COUNT          406 a          771 c          755 g          397 t
ORIGIN      
        1 cggcgtcgcc gagaaggtcg tgttcgagtc gtgtgctcat gtggtctcca tttatgcttg
       61 tgggtgatgc atcgccggct caggtgccgg cgacaaaact acggatgccg gaaccgacga
      121 actggacgcc gatgcagacg agcagaaagc cgttcaggcg ggcgacgacg ttgatgccct
      181 cgctgcccag gcgccgggaa acccagcgtg ccgagcgcag caccagccag atcagcagac
      241 aggtggcggc aatgctcgcc accgtcgccg tataggccag caggcggctg atgccgccat
      301 ccagttcggc gatctccgag gaaatgccga tcaccgtcgc cagtgccccc ggcccggcga
      361 tacccggcat cgccagcggg aaaaaggcaa agctgccgcc gctaccggtc agcgtcgtat
      421 cgctggtgcc gaacagcatg cggtagccga tgatggacac ggtcaggcca ccggcgatgc
      481 gcagggcacc gaaggagatg ccgaaggctt cgaggatcat cacgccggcg aacaatccac
      541 cggccagcag caggaagccg tagaagcacg cgcgccgggc ttccagcgta gcctccggcg
      601 ccgctcggcc ggccgccagg gaaagaaaga gcggaatctt cgacaaggga ttggtgatcg
      661 ttgccagact gatcaaaccg cccagaaaga aactgaccag cagtctgagc aggtgctcgt
      721 cgatgctcat tttcaggccg tccggaaacg ggcgatgccg gattcgagac tgcccgccag
      781 attgtccagt tgtcgcgccg tggtcgccat gctggcggtg gcccgattgt tttcatggat
      841 catctggacg atgtgctcga ctcgggcagc aatctcctcg ccgccggacg attgctggcg
      901 caggctggcc gagacgtcgt cgatgacctg ggcgacgtgg cggttgctgc tttcgatggc
      961 gctcatcgat tcgccggccg cgcaggccat gcgcagacct tcgtcgacgc ggctgaccga
     1021 gcgctgcatg tgctcggcca tcgagcgggc gccgttctgg atggcgctga ccatggcggc
     1081 gatctggctg gccgaagcgg tcgtcttttc ggccagcttg cgcacctcgt cggcgaccac
     1141 ggcaaagccc cggccgctgt cgccagcgcg cgccgcctcg atcgcggcat tgagcgccag
     1201 caggttggtc tggtcagcca gatcgtgaat caccgaggtg atcgccgaga tgcgctccga
     1261 ctcctgcccc agcgtcgttg ccgtcgctgc cgaatcggtg accagcccgg caatctgacg
     1321 aatctcgtcg accatggtct ggatcgtctg gcaaccaccg gccgcctgct gcccggcctt
     1381 gccggacaga tcctgggcct cctgaccgag gttggcgata tgcgtgatgc agaccgacat
     1441 ggtctgcagc gtttcggcca tggcggcact ggccgcggcc tgcgcttccg aaccgccgcg
     1501 catttcgtcg gcggaaccgg aaagcgcagt gacggcgtgc ttgagttgcc cggcttccgc
     1561 acggatggcg ccaagtatcc ccgccagcga ctgggtggtc ttcatcgccg ccgcctggac
     1621 acggcggatt tcggcaaagc cgttcggctc gatgcgctgc gtcaggtcgc cggcagcaat
     1681 gctttccatc aggcgagctg tcgtttccag cggacgacgc acgcccccgg tcagcagata
     1741 accgagaacg gcggcgatca ggacggcaac gaccaccagg atcagcgtca gccgacgggt
     1801 gtttgccgac tcggccacga tgtcgccggc ccgttcgcgg ctttgcgcct gcaccagctg
     1861 gcgcgcttcg gcgatgctgg cttcgagcac ctgcctgcgc ggctcggctt cctgctgcag
     1921 gatgcccatg gccaccgtcg acaggaactg catggcctcg gccatcgcct tgtcttggat
     1981 atcgatggcc gccgtcgcct gttgcctgac ccgttgcagc aacgcagctt caccgacgac
     2041 ggcatggtcg accgcaatct tctccagcgc cccgagggcg gcgaagaagc gcgccttctc
     2101 gcgctcgtag cgtgcggctt ccgccttcat cacctcggca tcggtgaaaa tgatgttgtt
     2161 gcgcacgctt tcacgcatac cggcataggc atagcccaac tcggcaatct ggctgtcctt
     2221 ggtcagcgac acggaggcga tgcgctcggc cgccgcatcg acacggccca gactgaaggt
     2281 cgtccagccg gcaacaatga cgaacagccc gacgacgacg gtaaacgcc
//
LOCUS       DAOSDX010000461         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_15986_length_2329_cov_3.615655, whole genome shotgun sequence.
ACCESSION   DAOSDX010000461 DAOSDX010000000
VERSION     DAOSDX010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15986_length_2329_cov_3.615655"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLN02_10160"
     CDS             <1..412
                     /locus_tag="PLN02_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOI52729.1"
                     /translation="QAWKVVDTLKGQGGGSLFLKTHPKSKNLWVDTALNPDPQVSQSI
                     AVFDIDNLGKGYQVLPIAEWAGVGEGAKRVLQPEYNLAGDEVWFSVWSAKNQQSAIVV
                     VDDKTRKLKAVIKDSRLITPTGKFNLHNTQHDVY"
     gene            465..785
                     /locus_tag="PLN02_10165"
     CDS             465..785
                     /locus_tag="PLN02_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOI52730.1"
                     /translation="MKFLILPVVLALGASLPLTAMASEALAKQGGCLACHAKDKKLVG
                     PAFKDIAAKYQGDAGAPAKLADKVRKGGSGVWGAIPMPPNPATRLGDADLKAVVAWIL
                     KGGV"
     gene            800..1117
                     /locus_tag="PLN02_10170"
     CDS             800..1117
                     /locus_tag="PLN02_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOI52731.1"
                     /translation="MRPCLALLLLPACLGAAAAAPDTAAERRLVHLVRQDCGSCHGLT
                     LKGGLGPPLTKAALADKPAEGLAQTILHGRPATPMPPFRGLLDETEAAWIVDRLQRGF
                     PAE"
     gene            1132..2325
                     /locus_tag="PLN02_10175"
     CDS             1132..2325
                     /locus_tag="PLN02_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOI52732.1"
                     /translation="MPAIPALARCRRLLACLPFLFLAACASQAPLRGSGGLGVVIERA
                     DGQVQIVDHLARRSLASVAGLGDLSHASVVFSRDGRYAYVFGRDGGLSKVDLLQARVE
                     RRIVQAGNSIGGAISQDGRLVVAQNYEPGGIKAFDAETLELLADVPADYGDGQRSKVV
                     GLADLSGNRFAYALFDAGEIRITDLSDPKQPRTQRFPAGKQPYDALVTPDGRYYIAGL
                     FGEDGLALLDTWQPELGTRRILAGYGRGEAPLPVYKMPHLRGWAIAGGRAYLPAIGRH
                     EVLVVDTTSWQEVGRIPVKSQPVFVMARPDGRQIWVNFAFPDNGWLQVIDPLTQQVSA
                     TLQPGRAILHLEFSAKGEQVWVSARDDNAVLVFDTASQRLLQTLPARSPSGIFFTQRA
                     FRMGF"
BASE COUNT          373 a          890 c          739 g          327 t
ORIGIN      
        1 gcaggcctgg aaggtcgtag acaccctgaa gggccagggc ggcggctcgc tgttcctgaa
       61 gacccatccg aagtcgaaga acctctgggt cgacaccgcg ctcaacccgg acccgcaggt
      121 cagccagtcg atcgccgtct tcgacatcga caacctcggc aagggctatc aggtgctgcc
      181 gatcgccgaa tgggccggtg tcggcgaagg tgccaagcgc gtgctgcagc cggaatacaa
      241 cctggccggc gacgaagtct ggttctcggt gtggtcggcg aagaaccagc agtcggcgat
      301 cgtcgtcgtc gatgacaaga cgcgcaaatt gaaggcggtg atcaaggact cgcgactgat
      361 cacgccgacc ggcaagttca acctgcacaa cactcagcac gacgtctatt gacccgtgcc
      421 gcgcggaacc ggcctggccg gttccgcttc cggaggaaaa aaccatgaaa ttcctgattc
      481 ttcccgtcgt cctcgccctc ggcgccagcc tgccgctgac cgccatggcg tccgaagcgc
      541 tggccaagca aggcggctgc ctggcctgcc acgccaagga caagaaactg gtcggcccgg
      601 cgttcaagga catcgccgcc aagtaccagg gcgatgccgg cgcgccggcc aagctcgccg
      661 acaaggtgcg caagggcggc agcggtgtct ggggggcgat cccgatgccg ccgaacccgg
      721 cgaccaggct cggcgacgcc gacctcaagg ccgtcgtcgc ctggatcctg aaaggcggcg
      781 tctgagcgcc ggccgcgcca tgcgcccctg cctcgccctg ctgctgctgc ccgcctgcct
      841 cggcgcggcg gcggcagccc cggacaccgc cgccgaacgc cggctggtgc acctggtgcg
      901 ccaggattgc ggctcctgcc acggcctcac cctcaagggc ggcctcggcc cgccgctgac
      961 caaagccgcc ctcgccgaca agccggccga aggtctcgcc cagaccatcc tgcacggccg
     1021 ccccgccact ccgatgccgc ccttccgcgg cctgctcgac gaaaccgaag cagcatggat
     1081 cgtcgaccgc ctgcagcgcg gcttcccggc cgaatgaaac ggactcccgc catgccagcc
     1141 atccccgccc tcgcccgctg ccgcaggctg ctcgcctgcc tgcccttcct cttcctcgcc
     1201 gcctgcgcca gccaggcacc gctgcgcggc agcggcggcc tcggcgtcgt catcgaacgc
     1261 gccgacggtc aggtacagat cgtcgatcac ctcgcccgcc gcagcctggc cagcgtcgcc
     1321 ggcctcggcg acctctcgca cgcctccgtc gtcttctcgc gcgacggccg ctacgcctac
     1381 gtcttcggcc gcgacggcgg gctgagcaag gtcgacctgc tgcaggcacg cgtcgagcgg
     1441 cgcatcgtcc aggccggcaa cagcatcggc ggcgccatct cgcaagacgg ccgcctggtc
     1501 gtcgcccaga actacgagcc cggcggcatc aaggccttcg acgccgaaac cctcgaactg
     1561 cttgccgacg tcccggccga ctacggcgac ggccagcgct ccaaggtggt cggcctcgcc
     1621 gacctgagcg gcaatcgctt cgcctacgcg ctgttcgacg ccggcgaaat ccgcatcacc
     1681 gacctgtccg acccgaaaca gccgcgcacc cagcgcttcc ctgccggcaa gcagccctac
     1741 gacgcgctgg tgacgccgga tggccgctac tacatcgccg gcctgttcgg cgaggacggc
     1801 ctcgccctgc tcgacacctg gcagccggaa ctcggcaccc gccgcatcct cgccggctac
     1861 ggtcgcggcg aagcgccgct gccggtgtac aagatgccgc acctgcgcgg ctgggcgatt
     1921 gccggcggcc gcgcctacct gccggcgatc ggccgccacg aggtgctggt ggtcgacacc
     1981 acgagctggc aggaagtcgg ccgcattccg gtcaagagcc agccggtctt cgtcatggcg
     2041 cggccggacg ggcgccagat ctgggtcaac ttcgccttcc ccgacaacgg ctggctgcag
     2101 gtcatcgacc cgctgaccca gcaggtgagc gccacgctgc agcccggacg ggcgatcctg
     2161 cacctcgaat tctcggccaa gggcgaacag gtctgggtct cggcgcgcga cgacaacgcc
     2221 gtgctggtct tcgacaccgc cagccagcgc ctgctgcaga cgctgccggc gcgcagcccg
     2281 agcggcatct tctttaccca gcgcgccttc cgcatggggt tctgagatg
//
LOCUS       DAOSDX010000462         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16008_length_2327_cov_3.274648, whole genome shotgun sequence.
ACCESSION   DAOSDX010000462 DAOSDX010000000
VERSION     DAOSDX010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16008_length_2327_cov_3.274648"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PLN02_10180"
     CDS             complement(<1..1165)
                     /locus_tag="PLN02_10180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI52733.1"
                     /translation="MLVNLFFVLAGWQHLGEARRLAFVTAGAKADRLSLALSRVCTAY
                     LQRADLVLESMGQAYRHARTTGGGESVAIAASLNGLAGQASGASAIGILNERGKRVFA
                     WRAEAGGLSLAPNLPPAMLNGELPLHVSPLQRDGRVVGLLLARRLLQLSGEFVGVAYA
                     YLDLGHFREALPGNELGAAGGAYLLDVEDRSLVAGFGGDGETKVLGELLAASDFLAAR
                     SAGQGSASLRLGPLSDGTERILSWHGVGGYPLVAAVALADVDFLAAWHERWVLVVVYS
                     LMLALVSLLGFVGWFQRRYAVGSHQAVPDSGAEARPGALPPADALTGLPEIGSLRGLL
                     RERIEQARLERRSLAVCHLNIDDFGSVNACYGREVGDSLLIQVAQCLQAGMRHH"
     gene            complement(1558..2112)
                     /locus_tag="PLN02_10185"
     CDS             complement(1558..2112)
                     /locus_tag="PLN02_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI52734.1"
                     /translation="MTTTEITVSKARAPAAAGAETEGGAEQQQYLTFTLGEEMFAIGI
                     LAIREIIEYGNLTEVPMTPPFIRGVINLRGAVVPVVDLAVRFGREPRPSSKRTCIVII
                     DIASANGTQEMGIVVDAVSEVLEIPDADIEPPPEFGARIRIDFISGMGKVNGRFVVML
                     DVERILSLDEVAVIASVGVHGGPA"
     gene            complement(2125..>2327)
                     /locus_tag="PLN02_10190"
     CDS             complement(2125..>2327)
                     /locus_tag="PLN02_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52735.1"
                     /translation="EEMSSQAGNLQELMAFFNLGNAPTRGTSGRGPARRAGGKSSGPA
                     ALGGHGQKRAAGEMGEADFTAF"
BASE COUNT          434 a          860 c          719 g          314 t
ORIGIN      
        1 cgtgatgccg catccccgct tgcaggcact gcgcgacctg aatcagcaga gaatcgccga
       61 cctcccggcc ataacaggcg ttcaccgagc cgaagtcatc gatgttcagg tgacagacgg
      121 caagcgaacg ccgctccagc ctggcctgct cgatcctttc ccggagcagg ccgcgcagcg
      181 agccgatctc gggcagaccg gtcagcgcat cggccggcgg cagcgcgcca ggccgagcct
      241 cggccccgct atcgggcacc gcctgatgac tgccgacggc gtagcggcgc tggaaccacc
      301 cgacgaaccc gagcagcgaa accagagcga gcatcagcga atacacgaca accagcaccc
      361 aacgttcgtg ccacgccgcg agaaaatcga catcggccag ggccaccgcc gcgaccaggg
      421 gatagccgcc aaccccatgc catgaaagaa tccgctcggt gccgtcggac agaggcccca
      481 ggcgcaatga cgcgctgccc tgcccggccg agcgcgcggc caggaagtcg ctcgccgcca
      541 acagctcgcc gagcactttc gtttcgccgt cgccgccaaa cccggccacg agggacctgt
      601 cttcgacatc aagcaggtag gcgccgcctg ccgcaccgag ttcgttgccc ggcaaggctt
      661 cgcggaaatg cccgagatcc aggtaagcat aggcaacgcc aacgaactcc cccgacaact
      721 gcaggaggcg cctggccagc aacaacccga cgacgcgacc gtcgcgctgc aggggtgaga
      781 catggagcgg cagttcgccg ttcagcatgg ccggcggcag atttggcgcc aacgacagcc
      841 cgcccgcctc cgcccgccac gcgaaaacgc gcttgccacg ctcgttcagg atgccgatgg
      901 cgctcgcccc ggaagcctgc cccgccagac cgttgagcga cgccgcgatc gccaccgact
      961 cgccgccccc ggtcgtccgg gcatgccgat aagcctggcc catgctttcc agaacgaggt
     1021 cggcccgctg cagataggcg gtgcagaccc gtgacaaggc gagcgacagc ctgtccgcct
     1081 tggcgccggc ggtcacgaac gccagccgcc gcgcctcgcc cagatgctgc catccggcaa
     1141 ggacaaaaaa cagattgacc agcaagacca ggccgatccc cctgagcatg ccccaggcac
     1201 gccagcgcag ccaggcagga cgcgaccgcg ccagcaagct tcccggcgcc gccgtcggcc
     1261 cgctcatact tccctcgcgc cccgggagcc gggccggtag atcgtcggct gcgccaagcg
     1321 cagcttcgcc gtcaccccag ccacctcgcc acgggccgcg cagcggtagt attcgccgca
     1381 atcggcagcc ctgtcgccgg gagacgccgc caggcggtga agcccgacga tggtgggtgc
     1441 cagcacgatt cgtcccgatt gattcatgac cgcttgtcca tccccttcag cctgccgccg
     1501 caacgcccgc cccgcggcgg gcgggacggg cagatgcggc gaaacggcaa actctcctca
     1561 agccggaccg ccatgcacac cgaccgaggc aataaccgcg acctcgtcga gcgaaaggat
     1621 gcgttcgaca tccagcatga cgacgaacct gccgttcacc ttgcccatgc cggagatgaa
     1681 atcgatccgg atacgcgccc cgaattccgg cggcggctcg atgtcggcat ccggaatctc
     1741 gagcacctcg gaaaccgcgt cgacgacgat tcccatctcc tgggtgccgt tggcggacgc
     1801 aatgtcgatg atgacgatgc aggtgcgctt ggaactcggt cgcggttcgc gaccgaagcg
     1861 cacggcaaga tcgaccaccg gcacgacggc gccgcgcagg ttgatgacgc cgcggatgaa
     1921 aggcggcgtc atcggcacct cggtcaggtt gccatactcg atgatctcgc ggatggcgag
     1981 gatgccgatg gcgaacatct cctcgcccag cgtgaacgtc agatactgct gctgctcggc
     2041 gccgccctct gtttcggcac cggccgccgc cggtgcccgt gccttcgaca cggtgatttc
     2101 agtagtcgtc atcaggcgct cctactagaa agcggtgaag tcggcttcgc ccatctcgcc
     2161 agccgcgcgc ttctgcccat ggccgccaag ggctgccggc cccgaggact tgccgccagc
     2221 gcgccgcgcc gggccgcggc cgctcgtccc gcgcgtaggc gcattgccga gattgaagaa
     2281 ggccatcaat tcctgcaggt tgccggcctg cgacgacatc tcctcgg
//
LOCUS       DAOSDX010000463         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16024_length_2326_cov_2.016292, whole genome shotgun sequence.
ACCESSION   DAOSDX010000463 DAOSDX010000000
VERSION     DAOSDX010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16024_length_2326_cov_2.016292"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PLN02_10195"
     CDS             <1..1201
                     /locus_tag="PLN02_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI52736.1"
                     /translation="AAEAAPSTVDAENGPKTVVDRKPRSFFSGRRLFGYAYRETLELR
                     RDPIRLAFALLGSALLMLVLGFGISLDVEGLRFAALDRDQSPESRAYIENIAGSRYFI
                     ERPPIRDDADLERRLRDGELALAVDIPPGFGRDLRRGHWPEVGLMVDGAMPYRGETVR
                     GYAEGMHLLYVQRLIRENTGVDSTYPANVVSRFRYNQDVKSIYAMVPAVIPILLVFIP
                     AILMALGVVREKELGSITNLYVTPVTRLEFLLGKQLPYIAVSLISFLLLVVQSQLVFQ
                     VPLKGSFAALAVGATLYVVATTGVGLLISTFTKTQIAALFGTAILTILPTVSFSGLTT
                     PVGALEGVSYWIGQLFPASYFLVISRGVYTKALGLSDLLPQFLALAAFIPVLTALSVA
                     LLKKQED"
     gene            1204..>2326
                     /locus_tag="PLN02_10200"
     CDS             1204..>2326
                     /locus_tag="PLN02_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI52737.1"
                     /translation="MRLPSLANIYRLGLKELKSLWADKTLLVLICWAFSGAIYTAATG
                     VSQELHNAPLAVVDEDRSPLSQRIVDAFYPPFFRPPERIELAAMDPGLDAGRYNFALV
                     IPANFQRDLRAGKVPDLQLNIDATIMSQAFIGATYIKRITLGEISEYLTGTRDESAGP
                     VRLVTRVRFNPNLKGFWFGGVMEAINNVTTLTIILVGAAFIREREHGTVEHLLVMPLT
                     PFEIMLAKIWANGLAVLVGVAFALAVMVERVLQVPIAGSLPLFLAAAACYLFAAASIG
                     IFLGTLARSMPQFGLLVILSIIPLLMLSGGISPRESMPQAVQDLMLAAPTTYFVRLAQ
                     GILYRGAGLEIVWRDLAAMTAIGAVFFAIALIRFRDAVTR"
BASE COUNT          360 a          896 c          651 g          419 t
ORIGIN      
        1 ggccgccgaa gctgcgccgt ccacggtcga cgcggaaaac gggccaaaaa ccgtagtcga
       61 tcgcaagccg cgcagtttct tcagcgggcg ccggctgttc ggctacgcct atcgcgaaac
      121 gctggaactg cgccgcgacc cgatccgcct cgcctttgcc ctgctcggtt cggcgctgct
      181 gatgctggtg ctcggcttcg gcatctcgct cgacgttgaa ggcctgcgct ttgccgcgct
      241 cgaccgcgat cagtcgccgg aaagccgcgc ctacatcgag aacatcgccg gctcgcgcta
      301 cttcatcgaa cgcccgccga tccgcgacga tgccgacctc gaacgccgcc tgcgcgacgg
      361 cgaactggcg ctggcggtgg atatcccgcc cggcttcggg cgcgacctgc ggcgcggcca
      421 ctggccggaa gtcggcctca tggtcgacgg cgccatgccc taccgcggcg aaaccgtgcg
      481 cggctacgcc gaaggcatgc acctcctcta cgtgcaacgc ctgatccgcg aaaacaccgg
      541 cgtcgacagc acctacccgg ccaacgtcgt ctcgcgcttc cgctacaacc aggacgtcaa
      601 gagcatctac gccatggtgc cggcggtgat tcccatcctc ctcgtcttca ttccggcgat
      661 cctgatggcg ctcggcgttg tgcgcgaaaa ggaactcggc tcgatcacca acctctacgt
      721 gacgccggta acgcgcctcg aattcctgct cggcaagcaa ctgccctaca tcgccgtgtc
      781 gctgatcagc ttcctgctgc tcgtcgtcca gtcgcaactc gtcttccagg tgccgctcaa
      841 gggcagcttc gccgcgctcg ccgtcggcgc cacgctctac gtcgtcgcca ccaccggcgt
      901 cggcctgctc atctcgacct tcaccaagac ccagattgcc gcgctgttcg gcaccgcgat
      961 cctgaccatt ctgcccaccg tcagcttctc cgggctgacc accccggtcg gcgcgctcga
     1021 aggcgtcagt tactggatcg gccaactctt tccggccagc tacttcctgg tcatcagccg
     1081 cggtgtctat accaaggcgc tcggcctgag cgacctgctg ccgcagttcc tcgcgctggc
     1141 cgccttcatc ccggtgctta ccgcgctgtc ggtggcgctg ctgaaaaagc aggaagactg
     1201 atcatgcgcc tgcccagcct cgccaacatc taccgcctcg gcctcaagga actgaagagc
     1261 ctgtgggccg acaagaccct gctcgtgctc atctgctggg ctttctccgg cgccatctac
     1321 accgccgcca ccggcgtctc gcaggaactg cacaacgccc cgctggccgt cgtcgacgaa
     1381 gaccgttcgc cgctctcgca gcgcatcgtc gatgccttct acccgccttt cttccgcccg
     1441 ccagagcgca tcgaactggc cgccatggac cccggactgg acgccggtcg ctacaacttc
     1501 gcgctggtca tcccggccaa cttccagcgc gacctgcgcg ccggcaaggt gcccgacctg
     1561 cagctcaaca tcgacgcgac gatcatgagc caggccttca tcggcgccac ctacatcaag
     1621 cgcatcacgc tcggcgaaat cagcgaatac ctcaccggca cgcgcgatga aagcgccggc
     1681 ccggtgcgcc tcgtcacccg cgtccgcttc aaccccaacc tcaagggctt ctggttcggc
     1741 ggcgtcatgg aagcgatcaa caacgtcacc acgctcacca tcatcctggt cggcgccgcc
     1801 ttcatccgcg aacgcgagca cggcaccgtc gagcacctgc tggtgatgcc gctcaccccg
     1861 ttcgagatca tgctcgccaa gatctgggcc aacgggctcg ccgtgctggt cggcgttgcc
     1921 ttcgcgctgg cggtgatggt cgaacgcgtg ctgcaggtgc cgattgccgg ctcgctgccg
     1981 ctcttcctcg ccgccgccgc ctgctacctg tttgccgccg catcgatcgg catcttcctc
     2041 ggcacgctcg cccgctcgat gccgcagttc ggcctcctcg tcatcctcag catcatcccg
     2101 ctgctcatgc tctccggcgg catctcgccg cgcgaaagca tgccgcaggc cgtgcaggac
     2161 ctaatgctcg ccgcccccac cacctacttc gtccgcctcg cccaaggcat cctctaccgt
     2221 ggcgccggcc tggaaatcgt ctggcgcgac ctcgccgcga tgaccgccat cggcgccgtc
     2281 ttcttcgcca tcgcgctgat caggttccgc gatgcggtga cgcgga
//
LOCUS       DAOSDX010000464         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16035_length_2325_cov_2.723348, whole genome shotgun sequence.
ACCESSION   DAOSDX010000464 DAOSDX010000000
VERSION     DAOSDX010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16035_length_2325_cov_2.723348"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLN02_10205"
     CDS             <1..833
                     /locus_tag="PLN02_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI52738.1"
                     /translation="LFLFFIEKCIRHLEPGGELVFIVPREFIKLTSARKLNAWLFGQG
                     SITHFFETGDVRVFAGANPNCCIFRFEKGRFDRRMADGRVFTEVDGQLMFLREDHGVR
                     LSDLFDVRVGGVSGADPVFRHAEGNLDFVCSKTADTGETRRMIYGIEHPALLPHKQRL
                     LARRVRKFDESNWWHWGRHFPINDDPRIYVNGRTRKSEPFFLHDCPNFDGSVLALFPK
                     NRRMRRRDLIECTMMLNHEVDWQQLGFVCDGRFLFTQRSLQTCLLPAKFSRYLPKEET
                     "
     gene            830..1642
                     /gene="pyrF"
                     /locus_tag="PLN02_10210"
     CDS             830..1642
                     /gene="pyrF"
                     /locus_tag="PLN02_10210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOI52739.1"
                     /translation="MTFTQQLAAAWQQNDSLLCVGLDPDPARFPAHLKGRDDAIFEFC
                     KSIVDATADLVCSFKPQIAYFAARRAEDQLEALIAHIHEQHPGVPVILDAKRGDIGST
                     AEQYAVEAFARFQADAVTVNPYMGRDSVEPYLAFPDKGVILLCRTSNPGGSDLQFLET
                     NGEKLYEHVARLAAEKWNSTGQIGLVVGATFPAEIARVRQIVGDMPLLVPGIGAQGGD
                     VEATVKAGRTAAGSGLMINSSRAILYAGHGEDYADAARQAALATRAEINQYR"
     gene            1654..>2325
                     /locus_tag="PLN02_10215"
     CDS             1654..>2325
                     /locus_tag="PLN02_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOI52740.1"
                     /translation="MRMDDYRESDNVEDRRGGGGISFGGGRLGIGSIAIALVASYFLG
                     VNPMTVLNLLSGGGMPVVQQQAPAQRPPANDETAVFVSKVLASTEDTWIPAFREMGKQ
                     YKEPRLVLYTGVTPTACGTGQAAMGPFYCPGDQKVYIDLAFFRELRERFHAPGEFAQA
                     YVVAHEVGHHVQHLLGISDQVHQAQQRASQAQANALSVRLELQADCLAGVWGKRTDSM
                     AQVLEP"
BASE COUNT          397 a          846 c          709 g          373 t
ORIGIN      
        1 acctgttcct gttcttcatc gagaaatgca tccgccacct cgaaccgggc ggtgaactgg
       61 tcttcatcgt gccgcgcgag ttcatcaagc tgacctcggc caggaagctc aacgcctggc
      121 tgttcggcca gggcagcatc acgcatttct tcgagaccgg cgacgtccgc gtcttcgccg
      181 gcgccaaccc caactgctgc atcttccgct tcgagaaggg gcgtttcgac cgccgcatgg
      241 ccgacggccg cgtcttcact gaagtcgacg ggcagctgat gttcctgcgc gaggatcacg
      301 gcgtgcgtct ttccgacctg ttcgacgttc gtgtcggcgg cgtttccggc gctgacccgg
      361 tgttccgcca cgccgagggc aacctcgatt tcgtctgctc gaagaccgcc gacaccggcg
      421 agacacggcg catgatctac ggcatcgaac accccgccct gctgccgcac aagcaacgcc
      481 tgctcgcccg ccgcgtacgc aagttcgacg agagcaactg gtggcactgg ggccgccatt
      541 tcccgatcaa cgacgacccg cgcatctacg tcaatggccg cacgcgcaag agcgagcctt
      601 tcttcctgca cgactgcccg aacttcgacg gttcggtgct ggcgctgttc ccgaagaacc
      661 ggcggatgcg tcggcgcgac ctgatcgaat gcacgatgat gctcaaccac gaagtcgact
      721 ggcagcaact cggcttcgtc tgcgacggcc gttttctgtt cacccagcgc agcctgcaga
      781 cctgcctgct gcccgccaag ttttcccgtt atctgccgaa ggaagaaaca tgacctttac
      841 ccagcaactc gccgccgcct ggcagcagaa cgactcgctg ctctgcgtcg gcctcgaccc
      901 cgatccggcc aggttcccgg cccacctgaa ggggcgcgac gacgccatct tcgaattctg
      961 caaaagcatc gtcgacgcca ccgccgacct cgtctgttcg ttcaagccgc aaatcgccta
     1021 cttcgcggcg cgccgcgccg aggaccaact ggaagcgctg atcgcccaca tccacgagca
     1081 acaccccggc gtgccggtga tcctcgacgc caagcgcggc gacatcggct cgaccgccga
     1141 gcagtacgcg gtcgaagcct tcgcacgctt ccaggccgac gccgtcacgg tcaatcccta
     1201 catggggcgc gattcggtcg agccttacct ggccttcccg gacaagggcg tgatcctgct
     1261 ctgtcgcacc tccaacccgg gcggctcgga cctgcagttc ctggaaacca acggcgaaaa
     1321 gctgtacgag cacgtcgccc gcctggccgc cgaaaagtgg aacagcaccg gccagatcgg
     1381 gctggtcgtc ggcgccacct tcccggccga gatcgcccgc gtgcgccaga tcgtcggcga
     1441 catgcccttg ctggtgccgg gcatcggcgc ccagggcggc gatgtcgagg ccaccgtcaa
     1501 ggccggccgt accgcggcgg gcagcggctt gatgatcaat tcctcgcgtg ccatcctcta
     1561 cgctggccac ggcgaggact acgccgacgc cgcccgccag gcggcgctcg ccacccgcgc
     1621 cgagatcaac cagtaccgct gaggagacgc cagatgcgca tggacgacta ccgggaaagc
     1681 gacaacgtcg aggatcgccg cggcggcggc ggcatctctt tcggcggcgg ccggctgggg
     1741 atcggcagca tcgccatcgc gctggtcgcc agctacttcc tcggcgtcaa tccgatgacc
     1801 gtgctcaacc tgctctccgg cggcggcatg ccggtcgtcc agcagcaggc gccggcgcag
     1861 cgcccgccgg ccaacgacga gacggcggtg ttcgtctcca aggtgctggc gtcgaccgag
     1921 gacacctgga ttcccgcctt ccgcgagatg ggcaagcagt acaaggaacc gcgcctggtg
     1981 ctgtacaccg gcgtcacgcc gaccgcctgc ggcaccggcc aggcggcaat ggggccgttc
     2041 tactgcccgg gcgaccagaa ggtgtacatc gacctcgcct tcttccgcga actgcgcgag
     2101 cgcttccacg cccccggcga attcgcccag gcctacgtcg tcgcccacga agtcggccac
     2161 catgtgcagc acctgctggg catttccgac caggtccatc aggcgcagca gcgggccagt
     2221 caggcgcagg ccaacgcgct gtcggtgcgc ctcgaactgc aggccgactg cctggccggc
     2281 gtctggggca agcgcaccga cagcatggcc caggtgctcg aaccg
//
LOCUS       DAOSDX010000465         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16073_length_2321_cov_2.482348, whole genome shotgun sequence.
ACCESSION   DAOSDX010000465 DAOSDX010000000
VERSION     DAOSDX010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16073_length_2321_cov_2.482348"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLN02_10220"
     CDS             complement(<1..480)
                     /locus_tag="PLN02_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOI52741.1"
                     /translation="MSDRKANDAGRAGTASPEAERSLFGEILDWMLAPLLFVWPLSIA
                     FTHYFANNVANFPYDQSLRELVATLSRQVQYVDNEPVVALHASTRALLRADEIDSVYF
                     HVVDESRKLLVGDRELPIPEGTGKGNLVPGEVYFRDIEFNGQELRIAYSYLLDPQLPD
                     "
     gene            complement(458..1129)
                     /locus_tag="PLN02_10225"
     CDS             complement(458..1129)
                     /locus_tag="PLN02_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI52742.1"
                     /translation="MRILIAEDDTIIADGLCRSLRQAGYAVDWAANGHDADTALLTVS
                     YDLLILDLGLPKLSGLEVLKRLRSRNSQLPVLILTALDGTHDRVKGLDLGADDYMVKP
                     FELAELEARVRALTRRSSGTAPTIVCGPLSFDQVGRVAQIDGVPLELSAREIGLLEVL
                     LSRMGRLVSKDQLVDHLCGWGEEVSHNAIEVYVHRLRKKLESGGVKIATVRGLGYCLE
                     RPQGE"
     gene            complement(1340..>2321)
                     /gene="actP"
                     /locus_tag="PLN02_10230"
     CDS             complement(1340..>2321)
                     /gene="actP"
                     /locus_tag="PLN02_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286194.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOI52743.1"
                     /translation="DFNPDALFSRATDLHRLKDALMAPGGLIKDPVSALSVGMALMFG
                     TAGLPHILMRFFTVADAREARKSVAWATMWIGYFYLLTFIIGFGAIVALIGHPGSHFV
                     GGRIASGLQGGANMAAIHLAGVLGGDLFLGFIAAVAFTTILAVVAGLTLSGASAVSHD
                     LYATVLRKGAATSEQERRVSKLSTFALGVVAVLLGIVFGRQNVAYIVMLAFSVACSVN
                     FPVLAMAVLWKDCTTRGVTVGGFLGLFAAVALTVLSPVVWESVLRHPHGSAPFPYASP
                     AIVSMPLAFLAIWLVSRFDRSPQAARERALFPDQLIRSETGIGASAARSH"
BASE COUNT          475 a          753 c          745 g          348 t
ORIGIN      
        1 gtccggtaac tgcggatcga gcaggtagga ataggcgatg cgcagttcct gcccattgaa
       61 ttcgatgtcg cggaaataga cttcgccggg caccagattg cccttgccgg ttccctccgg
      121 aatcggcaat tcccgatcgc cgaccagcag cttgcggctc tcgtcgacga cgtggaaata
      181 gacgctgtcg atctcgtcgg cgcgcagcag cgcccgggtc gaagcgtgca gggcgaccac
      241 cggctcgttg tctacgtact gtacctggcg actgagcgtg gcgaccagtt cgcgcagcga
      301 ctggtcgtag gggaaattgg cgacgttgtt ggcgaagtaa tgcgtgaaag cgatgctcag
      361 cggccagacg aagagcagcg gcgccagcat ccagtcgagg atctcgccga acagcgagcg
      421 ctcggcctcc ggcgacgccg tgccggcccg cccggcgtca ttcgccttgc ggtcgctcaa
      481 gacagtaacc caggccgcgg acggtggcga tcttgacccc gccggactcc agtttcttgc
      541 gcaggcggtg gacgtaaacc tcgatcgcgt tgtggctgac ttcctcgccc cagccgcaca
      601 ggtgatcgac tagctggtcc ttgctcacca ggcgccccat ccggctgagc aggacttcga
      661 gcagaccgat ctcgcgcgcc gagagttcga gcggcacacc gtcgatctgc gccacccggc
      721 cgacctggtc gaagctcaac ggaccgcaca cgatggtcgg cgccgtcccc gaggaacgcc
      781 gggtcagcgc tcgcacccgc gcttccagct cggccagctc gaacggcttg accatgtagt
      841 cgtcggcgcc gagatccagc cccttgaccc gatcatgcgt gccgtcgagc gcggtcagga
      901 tgagcaccgg caattgcgaa ttgcgcgagc gcaggcgttt cagcacttcg agaccggaaa
      961 gcttgggcag gccgaggtcg aggatcagca gatcgtagga aaccgtgagc agggcagtat
     1021 cggcgtcgtg cccgttggct gcccaatcga cggcataacc ggcctggcgc agcgaacggc
     1081 agagaccgtc ggcgatgatc gtatcgtcct cggcaatcag gatacgcatg atgcagcccc
     1141 cctggcggcg taagcatttt gtaagcgaat tgtagtaacc tgcacgggct gttctccttt
     1201 tatggtctcg gagcctcagg ttcatcctgg gctcaggggg taggcccgac ctgcactggg
     1261 caacccccta ccttcttttt cccggaactc gtcaagtcta acaaagaaaa cgcatcggcg
     1321 ttttccgggg cccgccggct cagtgcgagc gggcggccga cgcgccaatg ccggtttccg
     1381 agcggatcag ttgatccgga aacaaggcgc gctcgcgcgc cgcctgtggc gaacggtcga
     1441 agcgcgatac cagccagatc gccaggaaag ccagcggcat cgaaacgatc gccggcgaag
     1501 catacgggaa gggcgccgag ccatgcggat gacgcagcac gctctcccag accaccggcg
     1561 acagcacggt cagcgccacc gccgcgaaca agccgaggaa accaccgacg gtgacgccgc
     1621 gtgtcgtgca atccttccac agcaccgcca tcgccagcac cggaaaattg accgagcagg
     1681 cgaccgaaaa ggccagcatg acgatgtagg cgacattctg ccgcccgaag acgataccca
     1741 gcaggacggc gacgacgccg agcgcgaagg tggacagctt ggacacccgt cgctcctgct
     1801 ccgaagtcgc cgcccccttg cgcaacaccg tcgcatacag gtcatgcgac accgccgagg
     1861 ccccggacaa ggtcaggccg gcaaccacgg caagaatcgt ggtgaaggcg acagcggcga
     1921 tgaaaccgag aaagaggtcg ccgcccagca cgccagcgag atggatcgcg gccatgttgg
     1981 caccgccctg caggccgctg gcaatccggc cgccgacgaa atgcgatcca ggatggccga
     2041 tcagcgcaac gatggcgcca aagccgatga tgaaggtgag gaggtagaag tagccgatcc
     2101 acatcgtcgc ccaggccacc gatttgcgcg cctcgcgggc gtcggcgacg gtgaagaaac
     2161 gcatcaggat gtgcggcagg ccggcggtac cgaacatcag cgccataccg acggaaagcg
     2221 ccgaaaccgg gtccttgatc agaccaccgg gcgccatcag cgcgtccttc aggcgatgca
     2281 ggtcggtggc ccgggaaaac agcgcgtccg gattgaaatc g
//
LOCUS       DAOSDX010000466         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16078_length_2321_cov_1.905119, whole genome shotgun sequence.
ACCESSION   DAOSDX010000466 DAOSDX010000000
VERSION     DAOSDX010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16078_length_2321_cov_1.905119"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /gene="hscA"
                     /locus_tag="PLN02_10235"
     CDS             complement(<1..1266)
                     /gene="hscA"
                     /locus_tag="PLN02_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOI52744.1"
                     /translation="MALFQIAEPGESAAPHEHKLAVGIDLGTTNSLVATVRSGIAVCL
                     NDDQGRPLLPSVVHYHADGSTEVGHEARRNQALDPKNTIVSVKRFMGRGLKDIAHVEA
                     MPYDFVEGPGMVRVRTQAGVKSPVEVSAEILKVLKERGETALGGELVGAVITVPAYFD
                     DAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNAAEGVYAVYDLGGGTFDISILK
                     LTKGVFEVMATGGDSALGGDDFDHRIYCWAIEQGKLQPLSAEDARRLMMSCREAKEFL
                     TKNPEAPISLRLSTGENIDLRLDVATFAEITRTLVSKTLQPVKKALRDAGLRVEDVKG
                     VVMVGGATRMPQIQKAVGDFFRQDPLTNLDPDKVVAIGAATQANLLAGNKTGKDDWLM
                     LDVIPLSLGLETMGGLTEKIIPRNSTLPIA"
     gene            complement(1279..1812)
                     /gene="hscB"
                     /locus_tag="PLN02_10240"
     CDS             complement(1279..1812)
                     /gene="hscB"
                     /locus_tag="PLN02_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOI52745.1"
                     /translation="MSLDLTADFFSLFDLPKTFRLNLSELDSRYRDVQAQVHPDRFAS
                     APEAERRLSMQWATRANEAYMTLKKPLERAKYLLELAGHDLQAESNTAMPADFLMEQM
                     EWREAVMEARAGGDHHELERLFQRLTDDIAGRYEEVAGLLDDAQDYATVTDRVRRLMF
                     LEKLLFEIDDALASLED"
     gene            complement(1933..2256)
                     /gene="iscA"
                     /locus_tag="PLN02_10245"
     CDS             complement(1933..2256)
                     /gene="iscA"
                     /locus_tag="PLN02_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017478657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOI52746.1"
                     /translation="MAITLSDKAAKHVAGFLQKRGKGIGLRLGVRTSGCSGMAYKLEF
                     VDEVNEDDQVFDSNGVKVIVDARSLPYLDGMELDYAREGLNEGFKFNNPNVKDQCGCG
                     ESFNV"
BASE COUNT          397 a          743 c          729 g          452 t
ORIGIN      
        1 tgcgatcggc agggtcgagt tgcgcgggat gatcttctcg gtcagcccgc ccatcgtttc
       61 cagaccgagc gacagcggga tcacgtcgag catcagccag tcgtccttgc ccgtcttgtt
      121 gccggccagc aggttggcct gcgtcgcggc gccgatggcg acgaccttgt ccgggtcgag
      181 gttggtcagc gggtcctggc ggaagaaatc gccgaccgcc ttctggatct gcggcatgcg
      241 cgtcgcgccg ccgaccatga ccactccctt gacgtcttcc acgcgcaggc cggcatcgcg
      301 cagtgccttc ttgaccggct gcagggtctt cgaaaccagg gtgcgggtga tttcggcgaa
      361 ggtcgccacg tcgaggcgca gatcgatgtt ctcgccggtc gacaggcgca gcgagatcgg
      421 tgcttccggg ttcttggtca ggaattcctt ggcctcgcgg caactcatca tcagtcggcg
      481 cgcatcctct gcagaaagcg gttgcagctt gccctgctcg atcgcccagc agtagatgcg
      541 atggtcgaaa tcgtcgccgc cgagcgccga atcgccgccg gtggccatca cctcgaagac
      601 gcccttggtc agcttgagga tggaaatgtc gaaggtgccg ccgcccaggt cgtagaccgc
      661 gtacacgcct tccgccgcgt tatccagccc gtaggcgatg gcggcggcgg tcggttcgtt
      721 cagcaggcgc aggacgttga ggccggccag ccgtgccgcg tccttggtcg cctggcgctg
      781 cgcgtcgtcg aaataggccg ggacggtgat cacggcgccg accagttcgc cgccgagtgc
      841 cgtttcgccg cgttccttca ggaccttgag gatttccgcc gagacctcga ccgggctctt
      901 gacgccggcc tgggtccgaa cgcgaaccat gccggggcct tcgacgaaat cgtatggcat
      961 cgcctcgacg tgggcgatat ccttaaggcc gcgccccatg aagcgcttga ccgaaacgat
     1021 ggtgttcttc ggatcgagcg cctggttgcg ccgtgcttcg tggccgacct cggtcgaacc
     1081 gtcggcgtgg taatggacga ccgacggcag cagcggccgc ccctgatcgt cgttgaggca
     1141 gacggcgatg ccggaacgca ccgtggcaac cagcgaattg gtggtaccga ggtcgatgcc
     1201 gacggcgagc ttgtgttcgt gcggggcagc ggattcaccg ggttcggcga tctggaacag
     1261 ggccattggt cttttctttc agtcttccag cgacgccagc gcgtcgtcga tttcaaacag
     1321 caatttttcc aggaacatca ggcggcgcac ccggtcggtg acggtggcgt agtcctgcgc
     1381 atcgtcgagc agacccgcca cttcctcgta gcggccggcg atgtcgtccg tcaggcgctg
     1441 gaacagccgc tccagttcat ggtgatcgcc gccggcgcgc gcctccatca ccgcctcgcg
     1501 ccattccatc tgttccatca ggaaatcggc cggcattgcc gtattgcttt ccgcctgcag
     1561 gtcgtgaccg gccagttcga gcaggtactt cgcccgttcc agcggtttct tcagcgtcat
     1621 gtaggcttcg ttggcgcgcg tcgcccactg catcgacaaa cgccgctcag cctccggcgc
     1681 gctggcgaaa cggtccggat gcacctgcgc ttgcacatcg cggtaacgcg aatcgagctc
     1741 cgacaggttg agccggaagg tcttcggcaa gtcgaacagg gagaagaaat cagcggtcag
     1801 gtccaaagac aaacctcgga aaggcagcgc atcaggatgc gacaaacggc aaagcgaagg
     1861 cggggcaagg cgagccccgc gcagacagta cgtcagtacg gcaagcgggg ctcaacgcag
     1921 ccccgccgag ccttagacat taaaagattc gccacaacca cactgatcct tgacgttggg
     1981 attattgaac ttgaacccct cgttcaaccc ttcgcgggcg tagtccagtt ccatcccgtc
     2041 aagataaggc aggctgcgcg catcgacgat gaccttgacg ccgttgctgt cgaaaacctg
     2101 gtcgtcttca ttgacctcgt cgacgaattc cagcttgtag gccatgccgg aacagccact
     2161 ggtccttacg ccgaggcgca ggccgatgcc cttgccgcgt ttctgcagga aaccggcgac
     2221 gtgcttcgcc gccttgtcgc tcaaggtgat cgccattttc tattctccgt gcttcttctt
     2281 gtagtcggcc acggccgcct tgatggcgtc ctcggccaga a
//
LOCUS       DAOSDX010000467         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16104_length_2317_cov_3.524757, whole genome shotgun sequence.
ACCESSION   DAOSDX010000467 DAOSDX010000000
VERSION     DAOSDX010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16104_length_2317_cov_3.524757"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLN02_10250"
     CDS             <1..682
                     /locus_tag="PLN02_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOI52747.1"
                     /translation="ALNEAMRDWVTNIHNTFYIIGTVAGPHPYPMLVRDFQKIIGEEC
                     LEQMPEMAGRQPDAVIAAVGGGSNAMGIFYPYIDVPDVRLIGVEAAGSGIETGLHAAS
                     LTAGVPGVLHGNRTYLLQNEDGQIIETHSVSAGLDYPGVGPEHAWLKDIGRAEYQPIK
                     DDEALKAFHDLCRLEGIIPALESSHAIAYAMKLAPTMAKDKILLVNLSGRGDKDMHTV
                     AEKSGIVF"
     gene            762..1568
                     /gene="trpA"
                     /locus_tag="PLN02_10255"
     CDS             762..1568
                     /gene="trpA"
                     /locus_tag="PLN02_10255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOI52748.1"
                     /translation="MSRIQQTFARLNGEGRKALIPFITAGDPDADLTVPLMQALVEAG
                     ADIIELGVPFSDPMADGPTIQRASERALAKGMSLRKVLQLVTRFRTEDDKTPVVLMGY
                     ANPIEAMGLETFAEKAAQSGVDGVLVVDYPPEEAERFGAAMKAKGMDPIFLIAPTSSA
                     ERIAHTAKIASGYVYYVSLAGVTGSGALNVDAVAQKLPLIREQTGLPVGVGFGIRDAA
                     TAARIAAFADAVVVGSRIIEEIEQSTAETACNRVKALVADIRRGVDEVKT"
     gene            1565..>2317
                     /gene="accD"
                     /locus_tag="PLN02_10260"
     CDS             1565..>2317
                     /gene="accD"
                     /locus_tag="PLN02_10260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOI52749.1"
                     /translation="MSWLDKLLPPKIKREQGQRRSNLPEGLWSKCPACEAVLYATDLE
                     KNLNVCPKCGHHNRINARQRLDLLLDPEGRFEIGAEVLPVDTLKFKDSKKYPERLMEA
                     NRDTGESDSLVVMQGAIKNLPVVAAAFEFDFMGGSMGSVLGERFVRGVNAAIEQKTPF
                     ICVAASGGARMQEGLFSLMQMAKTTAALTRLSQIGQPFISILTDPTMGGVSASFAFVG
                     DVVIAEPNALIGFAGPRVIEQTVREKLPEGFQR"
BASE COUNT          409 a          770 c          755 g          383 t
ORIGIN      
        1 cgcgctcaac gaagcgatgc gcgactgggt caccaatatc cacaacacct tctacatcat
       61 cggcacggtt gccggcccgc atccttaccc gatgctggtg cgcgacttcc agaagatcat
      121 cggcgaggaa tgcctcgagc agatgccgga aatggccggg cgccagccgg acgcggtgat
      181 cgccgcagtc ggcggcggct ccaacgcgat gggcatcttc tacccctaca tcgacgtgcc
      241 ggacgtgcgc ctgatcggtg tcgaagccgc cggttccggc atcgagaccg gcctgcatgc
      301 cgcttcgttg accgccggcg tgcccggcgt gctgcacggc aaccgcacct acctgctgca
      361 gaacgaggac ggccagatca tcgagacgca ctcggtctcc gccggcctcg actatcccgg
      421 cgtcggcccg gaacacgcct ggctgaagga catcggccgc gccgaatacc agccgatcaa
      481 ggacgacgag gcgctcaagg ccttccacga cctctgccgt ctcgaaggca tcatcccggc
      541 gctggagtcg agccacgcca tcgcctacgc gatgaagctg gcgccgacca tggcgaaaga
      601 caagattctg ctggtcaacc tctccggccg gggtgacaag gacatgcaca ccgtggccga
      661 gaagtcgggg atcgtgtttt gatgccccca cgctcatttc attcgctgcc ccctgagggg
      721 gcgctggcct cccttggggc ggcccggcag gagtccgtct gatgtcgcgc atccaacaaa
      781 ctttcgcacg cctcaatggc gagggccgca aggcgctgat tcccttcatc accgccggcg
      841 atcccgacgc cgacctgacc gtgccgctga tgcaggcgct ggtcgaagcc ggtgccgaca
      901 tcatcgagct gggcgtcccc ttctccgacc cgatggccga tggtccgacc atccagcgcg
      961 cttccgagcg ggcgctggcg aagggcatga gcctgcgcaa ggtgctgcag ctggtgaccc
     1021 gcttccgtac cgaggacgat aagacgccgg tggtgctgat gggctacgcc aatccgatcg
     1081 aggcgatggg gctggaaacg ttcgccgaga aagccgcgca atcgggcgtc gatggcgtgc
     1141 tggtggtcga ttacccgccc gaggaagccg agcgcttcgg cgccgcgatg aaggccaagg
     1201 gcatggatcc gatcttcctc atcgcgccga cctccagcgc cgagcgcatc gcccacacgg
     1261 cgaagatcgc cagcggttat gtctattacg tgtcgctggc cggcgtgacc ggttccggcg
     1321 cgctcaatgt ggacgcggtg gcgcagaagc tgccgctgat ccgcgaacag accggtctgc
     1381 cggtcggtgt cggcttcggc atccgcgatg cggcgaccgc cgcgcgcatc gccgctttcg
     1441 ccgacgccgt cgtcgtcggc agccggatta tcgaagaaat cgagcaatcg accgcggaaa
     1501 ccgcctgcaa cagggtcaag gcgctggtgg cggacatccg ccgcggtgtt gatgaggtga
     1561 aaacatgagc tggctggaca aactccttcc cccgaagatc aagcgcgagc aggggcagcg
     1621 ccgttccaac ctgccggagg ggctgtggag caagtgtccg gcctgcgagg cggtgcttta
     1681 cgcgaccgat ctggaaaaga acctcaacgt ctgtccgaag tgcggccatc acaaccgcat
     1741 caacgcccgc cagcgactcg acctgctgct cgatccggaa ggccgcttcg agatcggcgc
     1801 cgaggtgctg ccggtcgata cgctgaagtt taaggactcc aagaagtatc cggagcgcct
     1861 gatggaagcc aaccgcgaca ccggcgagag cgactcgctg gtggtcatgc agggcgcgat
     1921 caagaacctg ccggtggttg ccgccgcttt cgagttcgac ttcatgggcg gttcgatggg
     1981 ttcggtgctc ggcgagcgct tcgtgcgcgg cgtcaatgcg gcgatcgagc agaagacccc
     2041 gttcatctgc gtcgccgctt ccggcggcgc gcgcatgcag gaaggcctgt tctcgctgat
     2101 gcagatggcc aagacgacgg cggcgctgac ccggctgtcg cagatcggcc agcccttcat
     2161 ttccatcctc accgatccga ccatgggcgg cgtttcggcg tccttcgcct tcgtcggcga
     2221 cgtggtcatc gccgagccca acgcgctgat cggcttcgcc gggccgcgcg tgatcgagca
     2281 gacggtgcgc gagaaactgc cggaaggctt ccagcgt
//
LOCUS       DAOSDX010000468         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16142_length_2312_cov_3.438635, whole genome shotgun sequence.
ACCESSION   DAOSDX010000468 DAOSDX010000000
VERSION     DAOSDX010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16142_length_2312_cov_3.438635"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2312)
                     /locus_tag="PLN02_10265"
                     /pseudo
     CDS             complement(1..2312)
                     /locus_tag="PLN02_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3-like element ISPa38 family transposase"
BASE COUNT          348 a          703 c          803 g          458 t
ORIGIN      
        1 ttcgtcccaa tgggcgcgga cgtgcttgat gttgagcgtg ccgccgatca tcggtttcaa
       61 cgcctcgtag gtggcatcgc ccttcgggat gtagagcttg gtgtcgccca ggtcacgaat
      121 gcgcggggcg aagcggaagc ccagcaggtg catcaacgcg aagacgtggt ccgtgaaccc
      181 tgccgtgtcg gtgtagtgct cctcgatccg caggtcggat tcgtgataca gcaggccgtc
      241 gagcacttag gtcgagtcgc gcacgccgac gttcacgacc ttggtgtgga acggcgcgta
      301 ctggtcggag atatgggtgt agaacgtccg ccctgggctg ctgccgtatt tcggattgat
      361 gtggccggtg ctctcggcct tgctgccggt gcggaagttc tggccgtccg acgatgacgt
      421 ggtgccgtcg ccccaatgct cggcgaaggg atgtcggaac tgcgcgttga ccagctcggc
      481 cagtgccgcc ccgtaggttt cgtcgcggat gtgccaggct tgcagccagg ccagcttggc
      541 gtaggtcgtg ccggggcacg attccgccat cttggtcagg cccaggttga tcgcgtcggc
      601 gaggatcgtg gtcagcaaca ggtttttgtc cttggccagg tcgcctgact tcaggtgggc
      661 gaagtgccgg gtgaagcccg tccattcgtc tacctccagc agcaattcgg tgatcttgac
      721 gtgcggtagg atcatcgccg tctggtcgat cagggcctgt gcggtatcgg gcaccgccgc
      781 atcgagcggc gtgatcttca ggcccgactc cgtgatgatg gcgtccggca gctcgttggc
      841 cagcgccatg cggttgacgg tggcgagctg cgtttccagc agcgtcagcc ggtcatgcag
      901 gtactggtcg caatcggtgg ccacggccag cggcaattcg ctggcctgct tgaggctggc
      961 gaatttcgcg ggcggcacca ggtagtcctc gaagtccttg aactggcgcg agccttgcac
     1021 ccagatgtcg ccggagcgca gcgcgttctt cagctccgac agcgcgcaca gttcgtagta
     1081 gcgccggtcg atgccggtgt cggtcatcac cagcttctgc cagcgcggct tgatgaactc
     1141 ggtcggcgcg tcggtgggca ccttgcgggc gttgtcgctg ttcatgctgc gcagcacctc
     1201 gatggcgtcg agtacgtcct tggcggcggg cgcggcccgc aacttgagca cgtcgagaaa
     1261 ttccggcgcg tagcggcgca gcgtggcgta gctctcgccg atgcggtgca ggaaatcgaa
     1321 gtcctcgggt tgcgcgagcc gctgcgcttc ggtgacgctc tcggcgaaag catcccagga
     1381 catgacggcc tcgatggcgg cgaacggatc gcggcccgct tgcttggcct cgatcagcgc
     1441 ctggccgatg cgcccgaaca gccgcacctt ggcattgatc gccttgccgg atgcctggaa
     1501 ctgctgctga tgcttgttct tggcggcatt gaacagcttg cccaggatgc ggtcatgcag
     1561 gtcgatgatt tcgtcggtga cggtggccat gccctcgatg gcgagcgcca ccagggtcgc
     1621 gtaacgccgc tgcggctcga acttcgccag gtcggcgggc gtcatctggc cgccctcgcg
     1681 ggcgatcttg agcagccggt tctggtgaac cagccgctcg atgccggagg gcaggtcgag
     1741 cgcctgccac gccttgaggc gttcgatgtg ttccagcatg tgccgcgagt tcggtttgac
     1801 cggggattgc cgcagccagg ccagccacgt cgtcttgccg ttgtcgcggc gcttgagcag
     1861 atcgtcgagg cgacggcgat gcacgtccgt cagcggctca gccaaggcgt cgtagagacg
     1921 ccggttggcg cgggtaatcg cttcggcgct cgcccgctcg acggcgttga gggcgggcac
     1981 aatgaccgac tgccgccgca ggtgctcgat caaggctctg gccagcacga tgcccttgtc
     2041 ggtttgcatg gccagctcgg tcagcaactg gacagcctgc cggtagtggc caatcgtgaa
     2101 cggctggaag ccgaacaccg tttgcagctc gaccaggtgc tcgcgtcggg tctgctcacg
     2161 ctgcccgtac tcgtcccagc tttcgatgcc gaccttgagc tggttggcga ccagtctcag
     2221 caatggcggg aacggtggct catcagcgcc aaggatgacg ccgggaaagc gcaggtagca
     2281 gagctgcacc gcgaagccca gccgattggc cg
//
LOCUS       DAOSDX010000469         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16149_length_2311_cov_2.540780, whole genome shotgun sequence.
ACCESSION   DAOSDX010000469 DAOSDX010000000
VERSION     DAOSDX010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16149_length_2311_cov_2.540780"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..856)
                     /gene="bioC"
                     /locus_tag="PLN02_10270"
     CDS             complement(95..856)
                     /gene="bioC"
                     /locus_tag="PLN02_10270"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HOI52750.1"
                     /translation="MSQPPKARIRQSFERAAATYDAAADVQRRICARLAAGMSDAAPA
                     RLLDAGCGTGAARGLLQARYPAAEIVALDLSTAMLRRLPHDCQRLAGDLEHLPLASAS
                     IDLYWSSLAVQWCDPARALGEARRVLQPGGRLALATLGPATFHEMRSAFRDVDAYRHT
                     LHFHDAEQLAALAGAAGFTAVDCVAYRETAFYPDLRALLRAVKAVGANQLGAGRRSGL
                     MSRSAFARLTAAWETLRQPDGLPLTYDVILIHAQT"
     gene            complement(840..1562)
                     /locus_tag="PLN02_10275"
     CDS             complement(840..1562)
                     /locus_tag="PLN02_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOI52751.1"
                     /translation="MSAPLVFLPGWCLGRGPLQTAVDALHGQFIDLPGYRNEPLETAF
                     DRAVAVLGQRLPSGTTLAGWSIGAQLALAVAARHPDKVGKLLLVAGTASFVQRDGWPD
                     AMPPATLADFSAAVGVDPAGMLPRFVGGFNRGDAQAKEVSRRILEQADPLPSSLVLAA
                     GLAWLRDVDLRTDAARVQAPTLLIHGDADPLMPPAAARALAALIPGARLELFAGCAHA
                     PFLSQAEHFVATARAFFDEPAA"
     gene            complement(1599..>2311)
                     /locus_tag="PLN02_10280"
     CDS             complement(1599..>2311)
                     /locus_tag="PLN02_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI52752.1"
                     /translation="HADLAALERLLAASPARTKLIVSDTVFSMDGDLAPLPALHALAE
                     RYDAWLVLDDAHGFGVLGPQGRGSLAHFGLPADPRILLMGTLGKAAGVGGAFVAGSAL
                     AIDYLRNKARSYIFTTAAPPAIACALSASLDLIATGDALRANLFARIAQLRDGLAGLP
                     WHLPASPTAIQPLIVGDNAAALALARRLWEKGLWVPAIRPPTVPQGTARLRISVSAAH
                     TEADIAALIGALREAAAG"
BASE COUNT          333 a          731 c          963 g          284 t
ORIGIN      
        1 cagagcccgg cgcggcgggc gcggtggagc agggcgcagg cgatgtgggt cttgccgaca
       61 tcggtgtcgg tgccggtgag gaagtaagct gaactcatgt ttgggcgtgg atcaggatga
      121 catcgtaggt gagcggcagg ccgtccggct ggcgcaaggt ttcccaggcg gcggtcaggc
      181 gggcgaaggc gctgcggctc atcagcccgc tgcgccggcc ggcgccgagc tggttggcgc
      241 cgaccgcctt gaccgcccgc agcagcgcgc gcagatccgg gtagaaggcg gtttcccggt
      301 aagcgacgca gtcgaccgcg gtgaaaccgg cggcgccggc gagcgcggcg agctgttcgg
      361 catcgtggaa atgcagcgtg tgccggtagg cgtcgacgtc gcggaaggcg ctgcgcatct
      421 cgtggaaagt cgccggaccg agcgtcgcca gcgccagtcg gccgcccggt tgcaatacgc
      481 ggcgggcttc gccgagggcg cgggcgggat cgcaccactg cacggccagg ctagaccagt
      541 agagatcgat gctggcgctg gccagcggca ggtgttcgag gtcgccggcg aggcgttggc
      601 agtcgtgggg caggcggcgc agcatcgccg tcgacaggtc gagggcgacg atctcggcgg
      661 ccgggtagcg cgcctgcagc agaccgcgcg cggcgccggt gccgcagccg gcgtcgagca
      721 ggcgggcggg cgccgcgtcc gacattccag cggcgagccg ggcgcagatg cggcgctgga
      781 cgtcggcggc ggcgtcgtag gtggcggcgg cgcgttcgaa cgactggcgg atgcgcgcct
      841 taggcggctg gctcatcgaa gaaagcgcgg gcggtggcga cgaagtgttc cgcttgcgag
      901 aggaagggag cgtgggcgca gccggcgaac agttcgagac gggcgccggg aatcagcgct
      961 gccagcgcgc gcgccgcggc gggcggcatc agcgggtcgg cgtcgccgtg gatgagcagg
     1021 gtcggcgcct gcacccgggc ggcgtcggtg cgcaggtcga cgtcgcgcag ccaggccagg
     1081 ccggcggcga ggacgagcga cgagggcagc ggatcggcct gttcgaggat gcggcggctg
     1141 acttccttgg cctgcgcgtc gccgcggttg aagccgccga cgaagcgcgg cagcatgccg
     1201 gccgggtcga cgccgacggc ggcgctgaaa tcggccagcg tggccggcgg catggcgtcc
     1261 ggccagccgt cgcgctggac gaaggacgcg gtgccggcga ccagcagcag cttgccgacc
     1321 ttgtccgggt ggcgggcggc gacggctagc gccaactggg cgccgatcga ccagccggcg
     1381 agcgtcgtgc cggacggcag gcgctggccg agcacggcga cggcgcggtc gaaagcggtc
     1441 tccaggggtt cgttccgata gccgggcaga tcgatgaatt gtccatgcag cgcgtcgacc
     1501 gcggtctgca gcgggccacg gccgagacac cagccgggca ggaaaacgag gggagcgctc
     1561 atcgcaacaa gctcatcgga gaaagtcggg cggcgatttt aacccgccgc cgcctcgcgc
     1621 agcgcgccga tcagggcggc gatgtcggcc tcggtgtgcg cggcggagac cgagatgcgc
     1681 aggcgcgccg tgccctgcgg cacggtcggc gggcggatcg ccggcaccca tagccctttt
     1741 tcccagagcc ggcgggcgag tgccagtgcc gccgcgttgt cgccgacgat cagtggctgg
     1801 attgccgtcg gcgaggccgg caggtgccag ggcaggccgg ccagaccatc gcgcaactgg
     1861 gcgatgcggg cgaagagatt ggcgcgcagc gcgtcgccgg tggcgatcag atcgaggctg
     1921 gcgctcagcg cgcaggcgat ggccggcggt gcggcggtgg tgaagatgta gctgcgcgcc
     1981 ttgttgcgca ggtagtcgat ggccagcgcc gagccggcga cgaaggcgcc gccgacgccg
     2041 gccgccttgc cgagggtgcc catgagcagg atgcgtgggt cagccggcag gccgaagtgg
     2101 gccaggctgc cgcgcccctg cgggccgagg acgccgaagc cgtgcgcgtc gtcgagcacc
     2161 agccaggcgt cgtaacgctc ggccagcgcg tggagcgccg gcagcggggc gaggtcgccg
     2221 tccatgctga acacggtgtc gctgacgatc agcttggtcc gcgccggact ggcggcgagc
     2281 aggcgctcca gcgcggcgag gtcggcgtgc g
//
LOCUS       DAOSDX010000470         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16181_length_2307_cov_3.719361, whole genome shotgun sequence.
ACCESSION   DAOSDX010000470 DAOSDX010000000
VERSION     DAOSDX010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16181_length_2307_cov_3.719361"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLN02_10285"
     CDS             <1..763
                     /locus_tag="PLN02_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOI52753.1"
                     /translation="TGERGKVVLFVDTFTHYYTPEIAAAALKVLRAGGYAVDVLEPAK
                     TDAEPERPLCCGRTYLSQGMVEEAKLEARRVMAALAPALAAGTPIVGLEPSCLLALRD
                     EFYSLGLGPDVGQLSKQAFLFEEFLMREGNKGLKLPLKPIPGGRALVHGHCHQKAFGA
                     MKSVRKVLGWIPEFEFEIIDASCCGMSGSFGLEAEHHDASVQMAELALLPAVREAAAE
                     TLIVADGFSCRHQIKDGSGRDALHVAVLLERALAG"
     gene            1103..1519
                     /locus_tag="PLN02_10290"
     CDS             1103..1519
                     /locus_tag="PLN02_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52754.1"
                     /translation="MENKIPLPTDSIYKFYALFGLLVFVFCVGATIYISRSTNDLIFT
                     SSIEYETLKTIEKPTTVEVAKREGIEKRVEVALADKTFFLRALGVIAALGMLTMAYGF
                     LKWHREIQPIQDETARLQLAKLRHEVRALSENNSPE"
     gene            complement(1775..>2307)
                     /locus_tag="PLN02_10295"
     CDS             complement(1775..>2307)
                     /locus_tag="PLN02_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOI52755.1"
                     /translation="RQVEGLNRLIEDLRVLSLKESGHLELQRGRHPLAEELDSVLQVF
                     SPRLSALGFTLHQAIDAKLQVDCDPVRIRQALMALLENALQHATPGLLSVRLDHSEGL
                     CRLSVEDQGPGLPAELAASVFEAFRRGDPSRSNKTGGSGLGLAVVKAIAEAHGGKAYC
                     QTSDLGGSIFVIAWPG"
BASE COUNT          454 a          697 c          695 g          461 t
ORIGIN      
        1 aaccggcgag cgcggcaagg tcgtgctctt tgtcgatacc ttcacgcatt actacacgcc
       61 ggaaatcgcc gccgccgcac tgaaagtgct gcgcgccggc ggttatgcgg tcgacgtgct
      121 cgaaccggca aaaaccgacg ccgagccgga acgcccgctg tgctgcggcc ggacctacct
      181 gtcgcaaggc atggtcgagg aagccaagct cgaagcgcgc cgggtgatgg ccgcgctcgc
      241 cccggcgctc gccgccggca cgccgattgt cggcctcgaa ccctcctgcc tgctggcact
      301 gcgcgacgag ttctacagcc tcggccttgg tcctgacgtc ggccagctat cgaaacaggc
      361 cttcctgttc gaggaattcc tgatgcgcga aggcaacaag gggctgaagc tccccctcaa
      421 gccgattccc ggcggccgcg cgctggtgca tggccactgt caccagaagg ctttcggcgc
      481 gatgaagtcg gtcaggaagg tgctcggctg gattccggaa ttcgagttcg agatcatcga
      541 cgccagttgc tgcggcatgt ccggcagctt cggcctggaa gccgaacacc acgacgcctc
      601 ggtgcagatg gccgaactcg cgctgctgcc ggcggtgcgc gaagccgctg cggagacgct
      661 gatcgtcgcc gacggcttct cctgccggca ccagatcaag gacggcagcg ggcgcgacgc
      721 gctgcatgtg gcggtgctgc tggaacgggc gctggccggc tgaaaatgcc cggattttgc
      781 gatcgaccgt gaaatccgcg gaatctcgat tgtgttgacc acgaaatccc gttttatctg
      841 accagacgat attgacgtgt tggacaggtg gcgataggca gtattcggcc acaagcggcc
      901 agaagctgtc tctcacgaaa gcgtcgcata gcggacatag ggtctgtccg tatgattgct
      961 gattgataag gagaccggct tcggataatc tccatgtcat tcaaaacctt agcgacgatc
     1021 caatcgtgtc cgtattatta gtaatgaccc agacgatttt gctcgtgaat aactgttggg
     1081 ccactaatta acgctcctgc caatggaaaa caaaattccg cttccgaccg acagcatcta
     1141 taagttctac gcgttgtttg ggctgttggt gttcgtattt tgtgttggtg ccaccatcta
     1201 catctcgcgc tcaacgaacg atctgatctt cacctcatcc atcgaatacg aaacgctgaa
     1261 gacaattgaa aagccgacta ccgtcgaagt tgcgaagcgt gagggtattg aaaagagggt
     1321 tgaagtggcg ctcgctgaca aaactttttt tctgcgtgcc ctcggagtta tcgcagcact
     1381 aggcatgctc acgatggcgt acggatttct caaatggcat cgtgaaattc agcctattca
     1441 agacgaaaca gcgcgccttc aactggcgaa acttcgacat gaggttcgcg cgctttccga
     1501 aaacaatagt ccggagtaac gcccctatgg tgccaactag aacgggctct gatccaaatc
     1561 tagggagggc gttcgttgat ccgtggccca accctgtagt cgagtggacc tgcgcaataa
     1621 gcggcgcagt ccgctcgctt ctacgttgaa catctgcttt cacaactaac caactaccgc
     1681 tttggctcga ttcgagtcag tcaaacgccc ccgaatttgt ggccagccac cagcggtttc
     1741 gcaaaacccg gggattcccg tcaccccttg ggttctaccc tggccaggcg atcacgaaga
     1801 tgctgccgcc cagatccgag gtctggcagt acgccttgcc gccatgcgcc tcggcgatgg
     1861 ccttgaccac tgccaggccg aggccgctgc cgcccgtctt gttcgaacgt gagggatcgc
     1921 cgcgccggaa ggcttcgaag acgctggcgg cgagctcggc cggcaggccc ggcccctggt
     1981 cttcgacgct cagccggcaa agcccttccg agtggtcgag ccgcacgctc agcaggccgg
     2041 gggtggcgtg ctgcaaggcg ttctccagca gggccatcag cgcctggcgg atgcgcaccg
     2101 ggtcgcagtc gacctgcagc ttggcgtcga ttgcctggtg cagcgtgaat cccagggcgc
     2161 tgaggcgggg cgagaagacc tgcaacacgc tgtccagttc ttcggccagc gggtgccggc
     2221 cgcgctgcag ttcgaggtgg ccgctttcct tgaggctgag gacgcggaga tcctcgatca
     2281 ggcggttgag gccttcgacc tgacgca
//
LOCUS       DAOSDX010000471         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16198_length_2305_cov_4.050667, whole genome shotgun sequence.
ACCESSION   DAOSDX010000471 DAOSDX010000000
VERSION     DAOSDX010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16198_length_2305_cov_4.050667"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLN02_10300"
     CDS             complement(<1..329)
                     /locus_tag="PLN02_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOI52756.1"
                     /translation="MNNPGFDIATVERDTGLSKDVLRVWERRYGFPLPERDAHGERRY
                     SAEQVERLRLIKRLMDQGHRPGKLVAAPLETLAGLSPRRPRPAPPAGAETAADGLDEL
                     LDLIRRH"
     gene            434..1003
                     /locus_tag="PLN02_10305"
     CDS             434..1003
                     /locus_tag="PLN02_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOI52757.1"
                     /translation="MLSTLRRCLAAILFAITALVPAAARAADCPPLLEHRFADLKDGR
                     PLSLCQYTGKVILVVNTASYCGFTSQYEGLEALYARLRERGLVVLGFPSNDFGEQEPG
                     SNQQIADFCRSTYGVEFPMVAKTVVKGKDANPFYRQLAQLTGNPPQWNFHKYLISRDS
                     RRVLAFGSSTRPDEPAFLKQVEAFLDAAP"
     gene            1024..1824
                     /gene="folE2"
                     /locus_tag="PLN02_10310"
     CDS             1024..1824
                     /gene="folE2"
                     /locus_tag="PLN02_10310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOI52758.1"
                     /translation="MNAPEKLFLPDVQSTPDDRLLAIQKVGVKGLRYPVQLLAANGHS
                     QSTVATLAMTVGLGAAVKGTHMSRFIELLEERRGVLSQEGMFRLLEEMLLRLDASSGR
                     IEMRFPWFIRKQAPVSGVESLLDYDAAFIVEREEVRYPTLTLAVTAPVTSLCPCSKKI
                     ADYGAHNQRSLITLEVRLNAMLGIEELVRIAEDEASCEVFGLLKRPDEKWVTERAYDN
                     PKFVEDLVRDIALRLMKEPRIREWKVISENFESIHNHSAYAELHGSNA"
     gene            1834..>2305
                     /locus_tag="PLN02_10315"
     CDS             1834..>2305
                     /locus_tag="PLN02_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI52759.1"
                     /translation="MDRINRQRIAVVGAGISGLATAWLLSRRYDVTLFEAGNYLGGHT
                     NTVDVTVDGCTHPVDTGFLVFNERTYPNLIAMFALLGVDSVATEMSFAVSLRKPELEW
                     AGSNLATVFGQKRNLLRPAFWRMLGDILRFNRESTAWLLTHPGDSRSLGEFLDAG"
BASE COUNT          394 a          787 c          716 g          408 t
ORIGIN      
        1 tcgtgccggc gaatcaggtc gagcagttcg tcgagcccat cggcggccgt ttcggcgccg
       61 gccggcggcg ccgggcgcgg gcggcgcggg ctcagcccgg ccagcgtctc gagcggcgcg
      121 gcgaccagct tgccggggcg atgtccctga tccatcagac gtttgatcag gcgcaggcgc
      181 tccacctgct cggcgctgta gcgcctttcg ccgtgcgcgt cgcgttccgg cagcggaaag
      241 ccgtaacggc gctcccagac gcgtagcacg tctttcgaga ggccggtgtc gcgctcgacg
      301 gtggcaatgt cgaaacctgg gttgttcatt ctttgtcctg aacaaaagtt agacaaatcg
      361 ttgacaaagg ggttgtcgct gactatctta cgaacaaagc cctgttttgt ccaggacaaa
      421 ttcggagata agcatgttgt ccaccctgcg ccgctgcctc gccgccatcc ttttcgccat
      481 caccgcgctg gtcccggcgg cggcgcgggc ggcggattgt ccgccgctgc tcgagcatcg
      541 cttcgccgat ctcaaggacg gccggccgtt gtcgctgtgc cagtacaccg gcaaggtgat
      601 cctggtcgtc aataccgcca gttattgcgg cttcacctcg cagtacgagg ggctggaagc
      661 gctctacgcc cggctgcgcg agcgcggcct ggtggtgctc ggcttcccgt cgaacgattt
      721 cggcgagcag gagccgggca gcaaccagca gattgccgac ttctgccgct cgacctatgg
      781 cgttgaattt cccatggtgg cgaagaccgt agtcaagggg aaggatgcta atcccttcta
      841 ccggcagctg gcgcaactga ctggcaaccc gccgcagtgg aacttccaca agtacctgat
      901 cagccgcgac agccgccgcg tgctggcttt cggcagcagc acgcggcccg acgagcctgc
      961 cttcctgaaa caggtcgagg cgttcctcga cgccgccccc tgaacccatc cccggagaac
     1021 cccatgaacg cccccgaaaa gctcttcctg cccgatgtcc agtccacgcc cgacgaccgc
     1081 ctgctggcga tccagaaagt cggcgtcaag gggctgcgct acccggtcca gctgctggcc
     1141 gccaacggcc acagccagtc caccgtcgcc acgctggcga tgaccgtcgg cctgggcgcc
     1201 gcggtcaagg gcacgcacat gtcgcgcttc atcgaactgc tcgaagagcg gcgcggcgtg
     1261 ctcagccagg aaggcatgtt ccgcctgctc gaggaaatgc tgctgcgcct ggacgccagc
     1321 agcggccgca tcgagatgcg ctttccctgg ttcatccgca agcaggcgcc ggtctccggc
     1381 gtcgaaagcc tgctcgatta cgacgccgcc ttcatcgtcg aacgcgagga agtacgttac
     1441 ccgacgctga ccctggcggt gacggcgccg gtgaccagcc tctgtccgtg ctcgaagaag
     1501 attgccgact acggcgcgca caaccagcgc tcgctgatca ccctcgaggt ccgcctcaac
     1561 gccatgctcg gcatcgagga gctggtacgc atcgccgagg acgaggcgtc gtgcgaggtg
     1621 ttcgggctgc tcaagcggcc ggacgagaag tgggtcaccg aacgcgccta cgacaacccg
     1681 aagttcgtcg aggacctggt ccgcgacatc gcgctgcgcc tcatgaagga gccgcgcatc
     1741 cgcgagtgga aggtgatctc ggaaaacttc gaatcgatcc acaaccattc cgcctacgcc
     1801 gagctgcacg gcagcaacgc ctgaggagcc atcatggacc ggatcaaccg tcagcgcatc
     1861 gccgtcgtcg gcgccggcat ctcgggactg gccactgcct ggctgctgtc gcgccgctac
     1921 gacgtgaccc tgttcgaggc cggcaactac ctcggcggtc acaccaatac cgtcgatgtc
     1981 accgtcgatg gctgcacgca tccggtcgat accggtttcc tggtattcaa cgagcgtacc
     2041 tatcccaacc tgatcgccat gttcgccttg ctcggggtcg attcggtggc gaccgagatg
     2101 tcgttcgcgg tcagcctgcg caagcccgaa ctcgagtggg cgggcagtaa cctggccacc
     2161 gttttcggcc agaagcgcaa tctgctgcgc cctgccttct ggcgcatgct cggcgacatc
     2221 ctgcgcttca accgcgaaag caccgcctgg ctgctcacgc atcccggcga ttcgcgcagc
     2281 ctcggcgagt ttctcgatgc tggcg
//
LOCUS       DAOSDX010000472         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16216_length_2303_cov_2.873665, whole genome shotgun sequence.
ACCESSION   DAOSDX010000472 DAOSDX010000000
VERSION     DAOSDX010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16216_length_2303_cov_2.873665"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="PLN02_10320"
     CDS             complement(<1..2158)
                     /locus_tag="PLN02_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52760.1"
                     /translation="MKSRLLQRQLEELFGGEGDAGYRQWLTAARQQGASALADDVERL
                     LQRVDASFGALTGFAKWNSVLSGDALADWNLRSGTIDSGRQWKEMLGYAQADLDDSII
                     QWQRLLHPEDQRLLQSRMAAHVRNRDRWFELECRLRAKSGGWQWFMVRGAVAARDGDG
                     EPARMLVLQRDITQIKAAQADLIAAKETAESANKARGAFLANMSHEIRTPMNGIIGMT
                     ELALDTELDAEQRHYLKTVKSSAESLLTIVNDILDFSKIEAGRMEVEAVSFILSDVVM
                     EAVRVLAVNAHRKGLELVVDIRPDVPERLVGDPTRLRQVIINLLGNAIKFTETGEVVI
                     AVELARQAAGGAELHVAVRDTGIGVPKEKQRAIFDAFSQADVSTTRRFGGTGLGLAIC
                     AKLVQLMNGRIWLESESGQGSVFHFTVGLGGDEAMGAPPACTQSFAGRHALIVDDNPV
                     AGQCLRDLLLRLGLQASLVGDPVQAVAVMNKRRGMDYPFDYLFVDAAMPAPAGMALVE
                     TWRNSGRLERMMVMLTTENQRHDLGRLRELGVAVHLLKPVGVGDLEVALGLAEASGRG
                     EDETLRLADFDPGLGEAPAEAAGLRVLLAEDNPVNQELALRLLERRGYRVRVANNGAE
                     AVDMFDEGAFDVILMDLQMPVMGGIEATETIRSHEMRRSWVISHELKPIHIIAMTANA
                     MDSDRERCLAAGMNDYLSKPLRPEALFAALRRVGGEV"
     gene            complement(2164..>2303)
                     /locus_tag="PLN02_10325"
     CDS             complement(2164..>2303)
                     /locus_tag="PLN02_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOI52761.1"
                     /translation="LGERVEISHKASSRVPYAQGSLRAARFIAGRKNGLFDMQDVLGL
                     R"
BASE COUNT          435 a          825 c          646 g          397 t
ORIGIN      
        1 cgacctcgcc gccaacccgg cgcaaagccg caaacaaggc ttccggtcgc agcggcttgg
       61 acaggtaatc gttcatgccg gccgccagac aacgctcgcg gtcgctatcc atcgcattgg
      121 ccgtcatcgc gatgatgtgg atcggcttca gttcatggga aatcacccaa ctgcgccgca
      181 tttcgtgcga acggatggtt tcggtggcct cgatcccccc catgaccggc atctgcaggt
      241 ccatcaggat gacatcgaac gccccctcat cgaacatgtc gaccgcctcg gcgccattgt
      301 tcgcaacccg gactcgatag ccgcgccgtt cgagcagccg cagcgccagt tcctgattca
      361 ccggattatc ttccgccagc aacacgcgca acccggccgc ctctgccggc gcctcgccca
      421 aaccgggatc gaaatcggcc agccgcaagg tctcgtcctc accccgacca gaggcttccg
      481 ccagccccag cgcaacttcc agatcgccga ccccgaccgg tttcagcaga tgtaccgcca
      541 cccccagttc gcgcaggcgc cccagatcgt ggcgttgatt ctccgtcgtc agcatgacca
      601 tcatccgctc cagccgcccg gagttgcgcc aggtctcgac cagcgccatg cctgccggcg
      661 ccggcatcgc cgcatcgacg aacaggtaat cgaaggggta atccatgccg cgacgcttgt
      721 tcatcaccgc caccgcctgc accggatcgc caaccaggga agcctgcaaa cccagccgca
      781 gcagcaagtc gcgcagacac tgaccggcca ccggattgtc gtcaacgatc aacgcatgac
      841 gcccggcgaa agactgagta caggccggag gcgctcccat ggcctcatcg ccccccagtc
      901 cgacggtgaa gtgaaagacc gagccctgcc ccgactcgct ctccagccag attcgcccgt
      961 tcatcaactg caccagcttg gcgcagatcg ccagcccgag accggtgccg ccgaaacgcc
     1021 tggtggttga gacatcggcc tgggaaaagg cgtcgaaaat ggccctctgc ttttccttcg
     1081 gcaccccgat cccggtatcg cgcacggcaa cgtgcaactc cgcaccgcct gccgcttgcc
     1141 tcgcgagttc gacagcaatc accacctcgc cggtctcggt aaacttgatg gcgttaccaa
     1201 gcaggttgat gatcacctgg cgcaagcgcg tcggatcgcc gaccagccgc tccggcacgt
     1261 ccggacgaat atcgacgacc agttccagcc ccttgcgatg ggcattgacg gcgagcacgc
     1321 ggaccgcttc catgacgacg tcggacagaa tgaaggagac cgcttcgact tccatgcgcc
     1381 cggcctcgat cttggaaaaa tcgagaatgt cgttgacgat ggtaagcagc gactccgccg
     1441 acgacttgac ggttttcagg tagtggcgct gctcggcatc cagctcggta tcgagcgcca
     1501 gttcggtcat gccgatgatg ccgttcatcg gcgtccggat ttcatgactc atgttggcca
     1561 ggaaagcgcc gcgcgccttg ttggccgatt ccgccgtttc cttggcggcg atcaggtcgg
     1621 cctgcgccgc cttgatctgc gtgatgtctc gctgcaggac cagcatccgc gccggctcac
     1681 catcgccgtc gcgggccgcc accgcgccac gcaccatgaa ccattgccag ccgccactct
     1741 tggcgcgcaa gcggcattcc agttcgaacc agcgatcgcg gttgcgaaca tgtgccgcca
     1801 tccgcgactg cagcaggcgc tgatcttccg gatgcagcag gcgctgccac tggatgatcg
     1861 aatcgtcgag atcggcctgg gcgtagccca gcatttcctt ccactgccgt ccggaatcga
     1921 tagtgccgct gcgtagattc cagtcggcca gcgcatcgcc cgacagcacg ctgttccatt
     1981 tggcaaaacc ggtcagggcg ccgaaagaag catcaacccg ctgcagcaga cgctcgacgt
     2041 catcggccaa agccgacgcc ccctgctgcc gcgccgctgt cagccattgc cggtaaccgg
     2101 catccccttc gccgccgaac aactcttcga gctgacgctg cagcaaacgg gatttcatgg
     2161 tcgtcagcgc aagccgagca cgtcctgcat gtcgaacaag ccgtttttcc ggccagcaat
     2221 gaagcgtgcc gcgcgcaggc tgccctgcgc gtaaggcacc cgactgctcg ccttgtgcga
     2281 gatttcgacg cgctcgccga ggg
//
LOCUS       DAOSDX010000473         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16237_length_2301_cov_4.087266, whole genome shotgun sequence.
ACCESSION   DAOSDX010000473 DAOSDX010000000
VERSION     DAOSDX010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16237_length_2301_cov_4.087266"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLN02_10330"
     CDS             <1..315
                     /locus_tag="PLN02_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOI52762.1"
                     /translation="ELLRAIEAVRSGRHYLCPDAATLLTGNAALPGMLGSPPQTQLSP
                     RERAVLVQVAKGRTSTQIAEQMSIAPSTVEVHRRNIMRKLDLHSIAELTRYVISYGFD
                     QE"
     gene            656..>2301
                     /locus_tag="PLN02_10335"
     CDS             656..>2301
                     /locus_tag="PLN02_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI52763.1"
                     /translation="MLLSSREDPALWAEDLCHYQIELERQNRRLLDAKEESKQAPAEA
                     KSAPASSEARLRAILGSAPIGIAVMDAARRFIEVNPALCALTGYSADQLIGQSSRKLY
                     PDEASFARVGRDKLARFAQGATYEIELRMLRSDGHPLNVAMSAAPLEENDAGQLSTVI
                     IQDLTSSREIESELRLRVAALDAAANAIIVTEADGSIQWANAAFFEMSGYAAHETFGR
                     RPGELLRSGQQDPGEYERLWQTIASGRVWKGQLINRRKDGSLYHEFQTITPIPDRDGK
                     IRHYIAVKQDVSERVQNEQELAAYREHLEELVAERTADLQQARLNAERLSRVKSEFLS
                     NMSHEIRTPMNAVIGLAQVGARANRDRKCGENFRRILESGELLVAIIDDILDFSKIEA
                     GKLQLESRPVSLQRLIDRAVDLTVERARSKGIRFSIRKDDRLPAACLGDETRLLQILI
                     NLLSNAIKFTDQGSVVLEAGVVEARLMFRVIDTGIGMTQEQMGRLFSPFEQAETSIPR
                     RFGGTGLGLVICRRLVEAMGGEIRVDSRSGFGSVFEVRLPL"
BASE COUNT          466 a          664 c          726 g          445 t
ORIGIN      
        1 gaactgctgc gcgccatcga ggcggtacga agcgggcgcc attatctctg tcccgatgcg
       61 gcaaccttgt tgaccggtaa tgccgcgctc cccggcatgc tcggtagtcc accgcagacc
      121 cagctcagcc cccgtgagcg cgcggttctg gtgcaggtgg ccaagggccg cacctcgacc
      181 cagattgccg agcagatgag cattgcgccg tccacggtcg aggtgcatcg ccgcaacatc
      241 atgcgcaagc tcgatctgca cagcatcgcc gaactgaccc gttacgtcat cagttacggt
      301 ttcgaccagg aatagacggt ttattgcgga tttcacctac cggcttcctg gtaacaggaa
      361 tagcggtttt cgtgaatatc gggtgtcgac ggcagtcgat atcatctttg gcataacaga
      421 taagcatttt ctgaatattg ggcaccaaag aagctttgtg ctatttttgt tgttttagat
      481 agatgtgaca tctggtcttc acccgatttt cgctgcagtt tgtggcgaat tggatggtct
      541 caggggatag ggctggatgg acatcgaacc gaatacccgt ttgcaggagc cggcaatgct
      601 tgccgaacgc gtgcggagcg cgctacgcaa tggtgatatc gcagaggcaa ggaacttgct
      661 gctcagcagc agggaagatc ccgcgctctg ggcggaagat ctctgccatt accagatcga
      721 actggagagg cagaatcgcc ggttgctgga tgccaaggag gaaagcaagc aggcgccggc
      781 cgaggcgaag tccgcgccgg cgtcgagcga agcacgcttg cgcgccattc ttggttcggc
      841 gccgatcggc atcgcggtga tggatgcggc gcgccgcttc atcgaagtca atccggcctt
      901 gtgcgcattg accggctatt cggccgatca attgatcggt cagtcgtcac gcaagcttta
      961 tccggacgag gccagctttg ccagggtcgg gcgggacaag ctcgctcgtt tcgcgcaagg
     1021 ggcgacttac gagatcgagc tgcgcatgtt gcgcagcgac gggcatccgc tcaacgttgc
     1081 catgtcggcc gcgccgcttg aagaaaatga cgccgggcaa ctgtccaccg tgatcattca
     1141 ggacctcact tccagccggg agatcgagag tgaactgcgc ttgcgggtgg cggcgctgga
     1201 tgccgcggcc aacgcgatca ttgtcactga ggccgacgga agcattcaat gggccaatgc
     1261 cgcctttttc gagatgagcg gttatgcggc gcacgaaacc ttcggccggc gccccggcga
     1321 actgctgcgc agcggccagc aggaccctgg cgaatatgag cgcctctggc agacgattgc
     1381 cagtggtcgg gtctggaaag ggcaattgat caatcggcgc aaggacggca gcctctatca
     1441 cgagttccag acgatcacgc cgatccccga ccgcgatggg aagatccgcc actacatcgc
     1501 cgtcaagcag gacgtcagcg agcgcgtcca gaacgaacag gaactggcgg cctatcgcga
     1561 acacctcgag gaactggttg ccgagcgaac ggccgacctg cagcaggcgc gcctgaatgc
     1621 cgagcgtctg agccgggtga agagtgagtt cctgtccaac atgagccatg agattcgcac
     1681 gccgatgaat gccgtgatcg gcttggcgca agttggcgcc cgcgccaatc gcgaccggaa
     1741 atgtggagag aatttccgac ggattcttga atccggggaa ttgctggtgg cgatcatcga
     1801 cgacatcctg gatttctcga aaatcgaagc cggcaagctg cagctcgaat cgcgcccggt
     1861 cagcctgcag cgactgatcg accgggccgt cgatctgacg gtggagcgtg cgcggagcaa
     1921 aggcatccgc ttctccatcc gcaaggacga caggctaccc gcggcttgcc tgggcgacga
     1981 aacccggttg ttgcagatac tcatcaacct gctttccaac gccatcaagt tcaccgatca
     2041 gggaagcgtc gtcctcgaag ccggcgtggt cgaagcgcgc ttgatgttcc gtgtcatcga
     2101 caccggcatc ggcatgacgc aggaacaaat gggccgtctc ttctcgcctt tcgagcaggc
     2161 ggaaacatcg attccccgac ggtttggcgg caccgggctc ggtctcgtga tctgccggcg
     2221 tctagtcgag gcgatggggg gcgagattcg ggtggatagc cgcagcggat tcggcagcgt
     2281 gttcgaagtc cggcttcccc t
//
LOCUS       DAOSDX010000474         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16351_length_2287_cov_3.015233, whole genome shotgun sequence.
ACCESSION   DAOSDX010000474 DAOSDX010000000
VERSION     DAOSDX010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16351_length_2287_cov_3.015233"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>407
                     /locus_tag="PLN02_10340"
     CDS             <1..>407
                     /locus_tag="PLN02_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOI52764.1"
                     /translation="PCLDGIELAQQLAGLARPPALVFVTAYDEFALQAFELAAADYLM
                     KPVRAQRLLTALQKLPGTRPDPAVLAGIGQAVRGGGRTHLSCHERGRLLLVPVEEVLY
                     FRADLKYVAARTVEREYLLDESLTHLENEFPEQ"
     assembly_gap    408..417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(587..1144)
                     /locus_tag="PLN02_10345"
     CDS             complement(587..1144)
                     /locus_tag="PLN02_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52765.1"
                     /translation="MGILDQLKSLFGRPARAPEPVAKVVPMPVERRQRPRVDPPPGTC
                     VLIIDDSKTVVAVLRKFLRSAGLDTIECFDAESGLVQLQEKRPQLVFLDIMLPGMNGF
                     AALRAIRRDAQTRDTPVIMMSGNEQAMEQFFGTRIGADDFMKKPFNRQEVFFRIERLL
                     DEQGIPRRNTKPAAEQPASAHAISA"
     gene            complement(1316..1966)
                     /locus_tag="PLN02_10350"
     CDS             complement(1316..1966)
                     /locus_tag="PLN02_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOI52766.1"
                     /translation="MLAILFITHPEIDLAVSRHFYASDGIWRYNSGDFWTALPNLWVP
                     RLGRGVLVLLILVALIGRFRPDVVPPATRDSVAFLLVAALLGPLVGVEAIKALAERAR
                     PSDIATFDGARTFSPAFLPAGQCKRNCAFVSGHAATTAFLLLAPGWRRKRRLPWLLAA
                     IVATACTGLARLAVGAHFLSDVVFAWYASYAALWLSETLYRPSPLPPPASTAPRDT"
     gene            complement(1995..>2287)
                     /locus_tag="PLN02_10355"
     CDS             complement(1995..>2287)
                     /locus_tag="PLN02_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52767.1"
                     /translation="GNRTLIAHLQYELRDRQLSVARWNPDGSVRDHYELTQSLTLPPG
                     APLLYLSSTALPADIASRFARHEHLASLATGSRGPGTTRRLEIYLLDDFQKP"
BASE COUNT          370 a          688 c          839 g          380 t
ORIGIN      
        1 gccgtgcctg gacggcatcg agcttgccca gcagctcgcc gggctggcgc ggccgccggc
       61 cctggtcttc gtcaccgcct acgacgaatt cgccctccag gccttcgagc tggcggcggc
      121 cgattacctg atgaagccgg tgcgcgccca gcgcctgctg acggcgctgc agaaactgcc
      181 cgggacgcgc cccgatcccg ccgtgctggc cggcatcggc caggcggtgc gcggcggcgg
      241 gcgcacgcat ctgtcctgcc acgaacgtgg ccgtctgctg ctggtaccgg tcgaggaagt
      301 gctgtatttc cgtgccgacc tcaaatatgt ggcggcgcgc accgtcgagc gcgaatacct
      361 gctcgacgag tcgctgaccc atctggaaaa cgagtttccc gagcagtnnn nnnnnnngcc
      421 tgcaccgagc ggtgctggtg gcgcggcggg cgatcgccgg tttcgagaaa gccgccggcg
      481 acgatgccga ggcctacggc tgggcgctgc tgcgcggtgt ttcggagaag ttgccggtca
      541 gtcgccgcca gtgggtggcg gcgaaaggcg ggctggggaa tccggtttag gcgctgatcg
      601 cgtgggcgga ggccggctgc tcggcagccg gtttcgtgtt gcgtcggggg atgccttgtt
      661 cgtcgagcag gcgctcgata cggaagaaga cttcctggcg gttgaagggc ttcttcatga
      721 aatcgtcggc gccgatgcgg gtgccgaaga actgttccat cgcctgctcg ttgccgctca
      781 tcatgatcac cggcgtgtcg cgcgtctgcg catcgcggcg gatggcgcgc agggcggcga
      841 aaccgttcat gcccggcagc atgatgtcga gaaagaccag ttgcggacgt ttttcctgca
      901 actggacgag gccgctttcg gcatcgaagc attcaatggt atcgagaccg gccgagcgca
      961 ggaacttgcg cagcacggca accaccgtct tggaatcgtc gatgatcagc acgcaggtgc
     1021 ccggcggtgg gtcgacccgc ggccgctgcc gcctctccac cggcatcggg accaccttgg
     1081 cgaccggttc cggtgcgcgg gcggggcgcc cgaagaggga tttcaattga tcgaggatgc
     1141 ccatgaattt tctccgcttg gttcgattcc gacgatctgc ccgggctttc cggcggtgaa
     1201 cggaacagtt ccgttcgccg ggagcgacgc cgattcatgt cgtatgcatt tgaccacggg
     1261 gggcgcggca agtctagtgc tttttccccg cctggctcta cttcccgggt gggctctagg
     1321 tgtcgcgcgg cgctgtgctg gcgggcggcg gcaaggggga aggtcggtac agcgtttcgc
     1381 tcagccacag ggcggcgtag ctggcatacc aggcaaagac cacgtccgaa agaaagtgtg
     1441 ctccgactgc caggcgcgcc aagccggtgc atgccgtcgc gacgatggcg gcgagcagcc
     1501 agggcagtcg tcgtttgcgt cgccagccgg gggccaggag caggaaggcg gtggtcgccg
     1561 cgtgaccgct gacgaaggcg cagttgcgct tgcattgacc tgccggcagg aaggccggcg
     1621 aaaaggtccg cgcgccgtca aaggtcgcaa tgtccgaagg tcgggcgcgt tccgccaggg
     1681 ccttgatcgc ctccacgccg acgagcgggc cgagcaatgc ggcgacgagc aggaaggcga
     1741 cgctgtcgcg ggtcgcgggc ggcacgacgt ccggtcgaaa gcggccgatc agcgccacga
     1801 ggatgagcag cacgagcacg ccacggccga ggcgcggcac ccacagattg ggcaaggcag
     1861 tccagaaatc gccgctgttg tagcgccaga tgccatcgct cgcatagaag tggcggctga
     1921 cggcgagatc gatctcggga tgggtgatga acaggatcgc cagcacaaga ccgacgccga
     1981 ggatcaggcg gtactcaagg cttctggaag tcatcgagca ggtatatctc gagccggcgc
     2041 gtggtccccg gtccgcgcga accggtcgcg agactggcga ggtgttcgtg gcgggcgaag
     2101 cggctggcga tatcggcagg cagggcggtg ctggagagat agagcagcgg tgcgccgggc
     2161 ggcagcgtca gcgactgggt cagctcgtag tggtcgcgca cgctgccatc ggggttccag
     2221 cgggcgacgg acagttgtcg atcgcgcagt tcgtattgca ggtgggcgat cagcgtgcgg
     2281 ttgccgg
//
LOCUS       DAOSDX010000475         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16407_length_2281_cov_3.216981, whole genome shotgun sequence.
ACCESSION   DAOSDX010000475 DAOSDX010000000
VERSION     DAOSDX010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16407_length_2281_cov_3.216981"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLN02_10360"
     CDS             <1..483
                     /locus_tag="PLN02_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52768.1"
                     /translation="LVAQGWGYLGDDIVALGRRPQDKGVEIHPFPSAIGLKPGSWPML
                     RPYYPAIDSLPVVPYADRQARFLPVGGNRVPTETRQCLRAILLPRYSPDAAVEIAPIS
                     PVQALCELIGAGVSTRQTAHMGHAEILLQMLEEVPAYKLTYSDLDAVASALESLTTCR
                     "
     gene            474..1559
                     /locus_tag="PLN02_10365"
     CDS             474..1559
                     /locus_tag="PLN02_10365"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOI52769.1"
                     /translation="MSLMRKDAHALLCRILAPASACTTIGLTPEDPRWTTLFELADAN
                     LVLPELLPQLIDKGLRESVPEPMLACLAEIDTLMAARSAELLAQMKEINGAFAEAGIL
                     PVWLKGAALMSEPEGQRRPRLMHDLDVWIPSPKEQTDALAVLAHLGYEMDSKAVATDW
                     QDSHHYAPLYHPERPMRLELHRHIVRKSFGDLLPDDRALARCEHATLDGLPIARLALA
                     DRVMHSLIQCSLMSTPPIERGQVKLMKLLDLARLIARGGPHHALAPEVVETLRASPLP
                     LRKPIQRFLTLFERDFLVANPLGKDTRYCDTVDYCLLHGHPPPSIMMRLLLQPPTSWR
                     QLLSEPGEIGNKIVRRIRQSLGQSTPA"
     gene            complement(1649..>2281)
                     /locus_tag="PLN02_10370"
     CDS             complement(1649..>2281)
                     /locus_tag="PLN02_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI52770.1"
                     /translation="PERSTNFEVVLEHYLPADAGVLGFNLYARQTSDFVERRVALEGA
                     RWVDRPYNEGDAEHWGAELDAKLNLAKLGVAPGTLKGHLTVPRSRVHDERLGLTREAR
                     ESPRYQLSLGYERPFGERVTAGFDLQHFAEVKTSIPGERQERTEDRTVLDLFAVYRLQ
                     PGLNLRFNANNLLRVDTCKLARAHSGANDWQLETCNGNVASYMLTLEGSW"
BASE COUNT          399 a          755 c          719 g          408 t
ORIGIN      
        1 ctggttgccc aaggctgggg ctatctgggc gacgacatcg tcgcactggg acgtcggccc
       61 caggacaagg gagtcgaaat ccaccccttc ccatcggcta tcggcctcaa gccaggaagc
      121 tggccgatgt tgcgccccta ttacccggcc atcgacagct tgcccgtcgt gccgtacgcc
      181 gaccggcagg cgcgctttct tccggtcggc ggcaaccggg ttccgactga aacgcgtcaa
      241 tgtctgcgcg ccatcctgtt acccagatat tcccctgacg cagcagtcga aatagcgcca
      301 atctcgcccg tgcaagccct gtgtgaattg attggcgccg gagttagtac gcggcagacc
      361 gctcacatgg gacacgccga aattctcctg cagatgcttg aagaggtgcc tgcctataaa
      421 ctgacctaca gcgaccttga cgccgtggcc agcgccctgg aaagcctgac gacatgtcgc
      481 tgatgcgcaa ggatgcccac gcgctgcttt gccgtatcct ggcccccgcc agcgcttgca
      541 cgacaatcgg gctcacaccg gaagacccgc gctggacaac gctgttcgag ctggcggatg
      601 ccaatctggt cctccccgag ctgctgccac agctaatcga caagggcttg cgggaaagcg
      661 tcccggaacc gatgctggcc tgcctggccg aaatcgatac gctgatggcg gcacgcagcg
      721 ccgaacttct ggcgcagatg aaggaaatca atggcgcatt cgccgaggcc ggcatcctgc
      781 cggtctggtt gaagggcgcg gcgctgatga gcgagcctga agggcaacgc cgcccaagat
      841 tgatgcacga cctcgatgtc tggattcctt cgcccaaaga acagactgat gccctggcgg
      901 tcctggcgca tctcggctac gaaatggaca gcaaggcggt ggcgaccgac tggcaggact
      961 cccatcatta cgcgccgctc taccacccgg agcgcccgat gcgcctggaa ctgcatcgcc
     1021 acatcgtgcg caagtcgttc ggcgacctgt tgccggacga ccgggcgctg gcgcgctgcg
     1081 aacacgccac cctggacgga ctgccgatcg cccgcctggc actggccgac cgggtcatgc
     1141 acagcttgat ccagtgctca ttgatgagca ccccgccgat cgaaagaggc caggtcaagc
     1201 tgatgaaact gctcgacctg gccaggctga tagcccgcgg cgggccgcat catgcgctgg
     1261 caccggaggt ggtggaaacg ctgcgcgcca gccctttgcc tttacgcaag ccgatacagc
     1321 gtttcctgac gctgttcgag cgcgatttcc tggttgccaa tccgctgggc aaggacacca
     1381 ggtactgcga caccgtcgat tattgcctgc tgcatggcca tccgcccccc tcgatcatga
     1441 tgcgcctcct gttgcagccc cccactagct ggcgccagct tctcagcgaa cccggcgaaa
     1501 tcggcaacaa gatcgtccga cgcattcgtc aatcgctcgg ccagagcact ccggcatagc
     1561 gcgacgccgt cccggccggc agggcttacc ggcgccgccc gggaattttc acgccgaata
     1621 cacacgccac ctcgggtggc ggtgtttcct accagctccc ctccagtgtc agcatgtagc
     1681 tggccacgtt gccgttgcag gtttccagtt gccagtcgtt ggcgccgctg tgggcgcggg
     1741 cgagtttgca ggtgtcgaca cgcagcaggt tgttggcgtt gaaacgcagg ttgaggccgg
     1801 gctgcaggcg gtagacggcg aacaggtcga ggacggtacg gtcctcggtg cgctcctggc
     1861 gctcgcccgg aatgctggtc ttcacttcgg cgaaatgctg caggtcgaag ccagccgtga
     1921 cgcgctcacc gaacggccgc tcgtagccga ggctgagctg gtagcgcggc gattcgcggg
     1981 cttcccgggt caggccgagg cgctcgtcgt gcacccggct gcgcggcacg gtcagatggc
     2041 cctttaaggt gccgggcgcc acaccgagct tggcgagatt gagcttggcg tcgagttcgg
     2101 cgccccagtg ttcggcgtcg ccttcgttgt acggccggtc gacccagcgc gcgccttcga
     2161 gcgccacccg gcgttcgacg aaatcgctgg tctgccgggc gtagagattg aagccgagca
     2221 ctccggcgtc ggccggcagg tagtgttcga gcaccacttc gaagttggtg ctgcgctccg
     2281 g
//
LOCUS       DAOSDX010000476         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16437_length_2278_cov_3.807467, whole genome shotgun sequence.
ACCESSION   DAOSDX010000476 DAOSDX010000000
VERSION     DAOSDX010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16437_length_2278_cov_3.807467"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..299)
                     /gene="rpsR"
                     /locus_tag="PLN02_10375"
     CDS             complement(24..299)
                     /gene="rpsR"
                     /locus_tag="PLN02_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOI52771.1"
                     /translation="MARFFKKKIDDKKKRRGNSLFKRRKFCRFTAEKVEQIDYKDVDV
                     LKEYIQENAKIMPARITGTSARYQRQLATAIKRARFLALLPYTDQHE"
     gene            complement(271..600)
                     /gene="priB"
                     /locus_tag="PLN02_10380"
     CDS             complement(271..600)
                     /gene="priB"
                     /locus_tag="PLN02_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HOI52772.1"
                     /translation="MNQITLSGRLVERKRLRYTPSGIPVSEARISHQSTLTENGSERQ
                     VEVEMAVVALGDTARWLEAAPLGSSIGIHGFLAARGRNSRTAVLHANTIEYLEGNENG
                     TILQEED"
     gene            complement(627..1001)
                     /gene="rpsF"
                     /locus_tag="PLN02_10385"
     CDS             complement(627..1001)
                     /gene="rpsF"
                     /locus_tag="PLN02_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOI52773.1"
                     /translation="MRHYEICFIVHPDQSEQVPGMVERYRSIVTTKGGTVHRLEDWGR
                     RQLAYPIQKIHKAHYVLMNIECDGETLAELEHSFRFNDAVLRHLTVKMKAAVTSPSPM
                     MKEEKSKSLLAGEAEAVVEAAA"
     gene            complement(1732..>2278)
                     /locus_tag="PLN02_10390"
     CDS             complement(1732..>2278)
                     /locus_tag="PLN02_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52774.1"
                     /translation="TTLDEHFEANPVMELADIMLSFGRVKGAAQALQEYIDNNPQEAL
                     QPWIRLMDVYRMAGMREEFEAVARNLNQHFNVEVQSWSDGKEAAEVDLVLDGGNAGGL
                     PASAAPTRAQALEDMPRIISSVVKMWDDEDVVGYLYELLRDNRGGQRQGFSLPVVEDI
                     LFLIELKETANRMEKEGGRSL"
BASE COUNT          442 a          680 c          637 g          519 t
ORIGIN      
        1 tgacttgcat gtcgggcctc cgctcactcg tgctggtcgg tgtagggcag cagcgccagg
       61 aaacgggcac gcttgatggc ggtggcgagc tggcgctggt agcgggcgct ggtgccagtg
      121 atgcgcgccg gcatgatctt ggcgttttcc tggatgtatt ccttgaggac gtcaacatcc
      181 ttgtagtcga tctgttcgac cttttccgcg gtgaagcggc agaacttgcg acgcttgaac
      241 aggctgttgc cgcggcgctt tttcttgtca tcaatcttct tcttgaagaa tcgtgccatt
      301 ttcgtttcct tccaaatatt cgatagtgtt ggcgtgcagc acggcggttc tgctgttcct
      361 gccccgggca gccaggaatc cgtggattcc gatgctgctg cctagcggtg cggcttccag
      421 ccagcgtgct gtatcaccca gagcgacaac tgccatttcg acttccacct gcctttcgct
      481 accgttttcg gtcagcgtcg attggtggct tatcctggct tcgctgaccg gaattccgct
      541 cggtgtgtag cgcaaacgct tgcgctcgac caggcggccg gacagggtga tctgattcag
      601 tacgctattc cccgtgcaat ccgcgatcag gcagcggctt cgacaaccgc ttcagcttcg
      661 ccggcgagca gcgacttcga cttctcttcc ttcatcatcg gcgaaggaga agtcacggcg
      721 gccttcatct tcacagtcag atggcgcaga acggcatcgt tgaagcggaa ggaatgctca
      781 agctcggcga gggtctcacc gtcgcactcg atgttcatca gcacgtagtg agccttgtgg
      841 attttctgga tcggataggc cagttggcga cgaccccagt cttccagacg gtgcaccgta
      901 ccacccttgg tggtgacgat ggaacggtag cgctcgacca tgccgggcac ttgttcgctc
      961 tggtccggat ggacgatgaa gcagatttcg tagtggcgca tgcatactcc ttttgggata
     1021 aaccctccgt catgcgtaac ggtagggcaa ggtggaaagc cggctatgat aacagctaac
     1081 accaaaccac aaaacagata acggacaaaa tcacgaaaaa tcaggaaaaa acacattgcg
     1141 tcttcccagg gaatcgaggc atgacgtttt gccgaaggag cagaagtgga ggtcgacgtt
     1201 gcttgatcga ctgtcgccaa acagtccgat ctccccctcg gccatgttga tccttccatt
     1261 cagggaagga cgcgtcttga atcgcgtagc gaacaagcca atggttcgct gctgatccgc
     1321 ctctcggaat ttcctgcagt ttccacccgt tgcggcgccc gccacggggg cttgcgggtt
     1381 gctgcatgag cgaagtcgac ggcgggtgcg gggtcgtgac tgctttgacc ggtgtgcctg
     1441 actggtatat gccggctcgg cggatggact gccatcactg cggcacccac ctagtggaac
     1501 acactggctg accggatcga cgctgccggg cgccccatcg gatgcgcgcc tgggtagagg
     1561 cactggtgca acttctgctg attgcccatg cggctcaact cagggggcac ggcctttgga
     1621 tttcgtcact cagcctgatt cttcctgaag atcaaggctg tattccccgg caaggcgcga
     1681 cgaaaaaaaa cggcatcgac tgccgttttt tttggggaac ccgggccaac ctcagaggct
     1741 acgcccacct tctttttcca ttcggtttgc cgtttccttg agttcaatca ggaacaggat
     1801 gtcctcgacg acaggcagcg agaaaccctg tcgctgccct ccccggttgt cgcgcaaaag
     1861 ctcgtacaga tatcccacca catcctcgtc atcccacatc ttgacgacac tactgatgat
     1921 cctcggcata tcttccaatg cctgcgcccg cgtcggcgca gcagatgcag gaagcccacc
     1981 cgcattccct ccgtcgagca cgaggtcaac ttccgccgcc tccttaccgt cgctccagga
     2041 ctgcacctcg acgttgaaat gctggttgag gttacgggca accgcctcga attcttcccg
     2101 catgccagcc atgcgataca catccatcaa ccgtatccat ggctgcaaag cctcctgcgg
     2161 attgttgtcg atgtattcct gcaaggcttg agcagctccc ttgacccgcc cgaaggaaag
     2221 catgatgtca gccagctcca tgaccggatt ggcctcgaaa tgctcgtcca gcgtcgtc
//
LOCUS       DAOSDX010000477         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16494_length_2272_cov_2.676590, whole genome shotgun sequence.
ACCESSION   DAOSDX010000477 DAOSDX010000000
VERSION     DAOSDX010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16494_length_2272_cov_2.676590"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="gatB"
                     /locus_tag="PLN02_10395"
     CDS             <1..399
                     /gene="gatB"
                     /locus_tag="PLN02_10395"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285889.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOI52775.1"
                     /translation="VMVDLAARLNKEGREIGESPVSAAQLGGLIQRIADNTISNNIAK
                     KVFEALWNGEGASADEVIEKQGLRQITDSGAIAALVDEVIAANPGNVAEYRAGKEKAF
                     NALVGQVMKAAKGKANPQQVNALLKEKLAG"
     gene            complement(365..1009)
                     /locus_tag="PLN02_10400"
     CDS             complement(365..1009)
                     /locus_tag="PLN02_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52776.1"
                     /translation="MPKLPFALLLIVLSAPSWGAEEYYCCTDPGNGRRACGDTLPAQC
                     RGRAYRVFDRAGNPIREVAAAMTPEEKAAAAIAAKRQQQLEEQRLERRRMDAALLSTY
                     ATPEDIDMAQSKAEGDVNLTIRDATEKITELQKRLNKLTAEAEFYKRSAMPSGLASDL
                     RTVNHEIRLQQELIEVKRKELAKIHAKYDGDRQRYYELTGRRSLSRPVSPSTAR"
     gene            complement(1002..1643)
                     /gene="pyrE"
                     /locus_tag="PLN02_10405"
     CDS             complement(1002..1643)
                     /gene="pyrE"
                     /locus_tag="PLN02_10405"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOI52777.1"
                     /translation="MDFRQEFIEFAVACKVLRFGEFTTKAGRQSPYFFNAGLFNDGAS
                     LGRLAGFYAKAAQTGGVAFDMLFGPAYKGIPLVAAIAIALAAGGSNYPFAFNRKEAKD
                     HGEGGSIVGAPLAGRVLIVDDVISAGTSVRESVELIRAAGATPAGVLIALDRQERGQG
                     ELSAVQEVQRDYGIPVIAVAGLRDLMTYLGNHPEFDAHRAAVALYRERYGVDA"
     gene            1686..>2272
                     /locus_tag="PLN02_10410"
     CDS             1686..>2272
                     /locus_tag="PLN02_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOI52778.1"
                     /translation="MLRIISLNLNGIRSAWSKNVEPWLAAQQADIVCFQELKAQAADL
                     SPDMLAPQGMHAFYHCAEKKGYSGVGIWTRQAPRGVIEGFDGGEFDAEGRYLRVDFGN
                     LSVISLYLPSGSSSPERQEAKFRFLDAFLPQMQALRAEGREIVICGDWNIAHREIDLK
                     NWKSNQKNSGFLPEERAWLTRVFDELGWVDVYRRL"
BASE COUNT          424 a          690 c          739 g          419 t
ORIGIN      
        1 gtgatggtcg atctggccgc ccgcctcaac aaggaaggtc gcgagatcgg cgagtcgccg
       61 gtcagtgcgg cgcaactggg tgggctgatc cagcgcatcg ccgacaacac catttccaac
      121 aacatcgcca agaaggtttt cgaggcattg tggaatggcg agggcgcgag cgccgacgag
      181 gtgatcgaga aacaggggtt gcgccagatt accgatagcg gggccatcgc cgcgctggtt
      241 gacgaagtca tcgccgccaa tccgggcaat gtcgccgaat atcgggccgg caaggaaaag
      301 gcgttcaacg ccctggtcgg tcaggtgatg aaggcggcca agggcaaggc caacccgcag
      361 caggtcaacg cgctgttgaa ggagaaactg gccggctgag cgagcggcgg ccggtcagct
      421 cgtagtagcg ctggcggtcg ccgtcgtatt tggcgtggat cttggccagt tccttgcgtt
      481 tgacctcgat cagttcctgc tgcagacgga tctcatgatt gaccgtgcgc aggtcgctgg
      541 ccaatcccga tggcatcgct gagcgcttgt agaattcggc ctcggcggtc agcttgttga
      601 gtcgcttctg caactcggtg attttctcgg tggcatcgcg gatcgtcagg ttgacgtcgc
      661 cttccgcctt gctctgcgcc atgtcgatgt cttccggcgt ggcgtaggtg gagagcaagg
      721 cggcgtccat gcgccgtcgt tccagccttt gttcctccag ttgctgctga cgtttcgcag
      781 cgattgcggc ggcggccttt tcttccggcg tcatggcggc ggcgacttcc cggatcggat
      841 tgccggcccg gtcaaaaacg cggtaagccc gtccccgaca ttgcgccggc agggtgtcac
      901 cgcaggcacg gcgaccgttg cccgggtcgg tgcagcagta gtactcctca gcaccccagc
      961 tcggggcgct cagaacgatc agcaggaggg caaagggaag tttaggcatc gacaccgtag
     1021 cgttcacggt agagggcgac ggcggcccgg tgcgcgtcga actccggatg attcccaaga
     1081 taggtcatca agtcgcgcag gcccgcgact gcgatcaccg ggatgccgta gtcgcgctgc
     1141 acttcctgaa ccgcggacag ctcgccctgg ccgcgttcct ggcggtcgag agcgatcagt
     1201 acgcctgccg gtgtggcacc ggcagcgcgg ataagttcca ccgattcgcg gaccgaggtg
     1261 ccagccgaga tcacgtcatc gacgatcagc acgcgcccgg ccagcggcgc gccgacgatg
     1321 ctgccgcctt cgccatgatc cttggcttcc ttgcggttga aagcgaacgg gtaattgctg
     1381 ccgcccgcag cgagcgcgat ggcaatggcg gcgaccagcg gaatgccttt gtaggccggg
     1441 ccgaacagca tgtcgaaggc gacgccgccg gtctgcgccg ctttcgcgta gaatccggcg
     1501 agccgcccga gcgacgcgcc gtcattgaac aggccggcgt tgaagaaata aggcgactgc
     1561 cgcccggcct tggtggtgaa ttcgccaaag cggagcacct tgcaggcgac ggcgaattcg
     1621 atgaattcct gacgaaaatc cataaatctc cagtatcagt cggaaaaacg accgagagtc
     1681 ccgcaatgtt acgcatcatc tccctgaacc tcaatggcat ccgctccgcg tggagcaaaa
     1741 acgtcgagcc ctggctggct gcacaacagg cggatattgt ctgctttcag gaactgaagg
     1801 cgcaagccgc cgatctgagt ccggacatgc tggcgccgca aggcatgcat gccttctatc
     1861 actgcgccga aaagaagggt tacagcggtg tcggcatctg gacacgacag gcgccgcgcg
     1921 gcgtcatcga aggcttcgac ggcggcgaat tcgatgccga agggcgctat ctgcgcgtcg
     1981 atttcggcaa cctgtcggtc atctcccttt acttgccttc cggttcttcg tcgcccgagc
     2041 gccaggaggc gaagttccgc ttcctcgacg ccttcctgcc gcagatgcag gccctgcgcg
     2101 cggaagggcg ggaaatcgtg atctgcggcg actggaacat cgcccaccgt gagatcgatc
     2161 tgaagaactg gaagtcgaac cagaagaact cgggcttcct gcccgaggaa cgggcctggc
     2221 tgacccgggt attcgacgag ctcggctggg tcgatgtcta tcgccgcctc ta
//
LOCUS       DAOSDX010000478         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16578_length_2264_cov_3.150747, whole genome shotgun sequence.
ACCESSION   DAOSDX010000478 DAOSDX010000000
VERSION     DAOSDX010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16578_length_2264_cov_3.150747"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLN02_10415"
     CDS             complement(<1..556)
                     /locus_tag="PLN02_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOI52779.1"
                     /translation="MSETTNILVVGIGGQGVMTATEILAEAAIALGNDAKKTEVAGMA
                     QRGGVVSSHLRFGPRVLSPQITPGSADVLLGFEAAEGMRWRHMLRPGGISLMNNARLV
                     PPVVELGLFDYPDDPIGEMRDGGGPVVAFDATSIALELGDIRLGNTVMLGAIADHLPF
                     PAEILLDCVLKRFQRKGEKLVELNR"
     gene            complement(553..>2264)
                     /locus_tag="PLN02_10420"
     CDS             complement(553..>2264)
                     /locus_tag="PLN02_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI52780.1"
                     /translation="AEWSVNEKVSLEVAIGAAYAGSRSFCCMKHVGMNVASDALMTLT
                     LTGVSGGLVIAIADDVGLSSSQNEQDSRHWGRFAHVPVFEPADSQEAYAMTLEAYELS
                     ERFQVPVILRMTTRINHVKALVTVGERQAHVGAGFRKDQARFVMVPGNAGKRIPLMFQ
                     RERDLRALAETSPLNFIEPGSDRRVGFIASGPAYMHVREAFPDAPVLKLGFSCPAPFE
                     KCRELAGMVERVVVVEEVEPLLETELKAQGIAVIGKAILPLQGELSPAVLKPAIARLL
                     GEALPETQAAAALQVFPRPPTMCVACPHLGVYYTLSQVRNLNISGDIGCYTLGAGHPW
                     QAMDTCVSMGASMGIAHGMDKGRGESDKDKRVVAVIGDSTFLHMGMQGLLDMVYNKGN
                     VTVLLLDNRAVGMTGGQDNPGNGRDIHGAEAPRIDFAKLVSALGVKDERIHVVNPYQL
                     PVLFKTIREEVKVPEVSVIITDQPCVLIKDYHKLKPYAVDEDKCTGCGNCIDVGCPAI
                     HVTRRGKEVKASGREVDLAFVRIESSVCTGCGLCLQPCAPKAIEHYVPSAPIQFAKGG
                     CQA"
BASE COUNT          393 a          736 c          749 g          386 t
ORIGIN      
        1 gccggttcag ttcgacgagc ttttcgccct tgcgctggaa gcgcttgagc acgcaatcaa
       61 gcaggatttc cgccggaaac ggcagatgat cggcgatggc gccgagcatc acggtgttgc
      121 cgagacggat gtcgcccagt tcgagggcga tgctggtcgc gtcgaaggcc accaccgggc
      181 caccgccgtc gcgcatttcg ccaatcggat cgtccggata gtcgaacagg ccaagctcga
      241 cgaccggcgg cacgaggcgc gcgttattca tcagcgagat gccacccgga cgcagcatgt
      301 gccgccagcg catgccttcg gccgcctcga aaccgagcag cacgtcggcg ctgcccggcg
      361 tgatctgcgg cgacagcacg cggggaccga agcgcaggtg cgaggaaacc acgccgccgc
      421 gctgcgccat gccggcgact tcggtcttct tggcatcgtt gccgagggca atggccgcct
      481 cggcgaggat ttcggtggcg gtcatgacgc cctgaccgcc gatgccgacg acgagaatgt
      541 tggtcgtctc gctcatgcct gacagcctcc cttggcgaac tggatcggcg cgctcggcac
      601 atagtgctcg attgccttcg gcgcgcaggg ctgcaggcag aggccacagc cggtacagac
      661 cgacgattcg atacggacga aagcgagatc gacctcgcgg ccactcgcct tgacttcctt
      721 gccgcgccgc gtgacgtgga tggccgggca gccgacgtcg atgcagttgc cgcaaccggt
      781 gcacttatcc tcgtcgaccg cgtaaggctt gagcttgtgg taatccttga tcaacacgca
      841 gggctggtcg gtgatgatga ccgagacctc gggcaccttg acctcttcgc ggatggtctt
      901 gaacagcacc ggcagctgat acggattgac gacgtggatg cgctcgtcct tgacccccag
      961 cgccgaaacc agcttggcga agtcgatgcg cggcgcttcg gcgccgtgaa tgtcgcggcc
     1021 gttgcccggg ttatcctggc cgccggtcat gccgacggcg cggttgtcga gcagcaggac
     1081 ggtgacgttg cccttgttgt acaccatgtc gagcaggcct tgcatgccca tgtgcaggaa
     1141 ggtcgaatcg ccgatcacgg caaccacgcg cttgtccttg tcggactcgc cgcgcccctt
     1201 gtccatgccg tgggcgatgc ccatcgaagc gcccatcgag acgcaggtat ccatcgcctg
     1261 ccaggggtgt cctgccccca gcgtgtagca gccgatgtcg ccggagatgt tcagattgcg
     1321 cacctgcgac agcgtgtaat agacgccgag gtgcggacag gcgacgcaca tcgtcggcgg
     1381 ccgcggaaaa acctgcaggg cagcagcggc ctgggtttcc ggcagcgctt cgccaagcag
     1441 gcgggcgatg gccggcttga gcacggccgg cgacagttca ccctgcagcg gcaggatggc
     1501 cttgccgatc accgcaatgc cttgcgcctt cagttcggtt tcgagcagcg gttcgacttc
     1561 ttcgacgacg acaacgcgct cgaccatacc cgccagttcg cggcactttt cgaacggcgc
     1621 cgggcaggag aagccgagct tcaataccgg cgcgtccggg aaggcttcgc gcacgtgcat
     1681 gtaggccggg ccggaagcga tgaaaccgac gcggcgatcg ctacccggtt cgatgaagtt
     1741 gagcggcgag gtttcggcca gcgcgcgcag gtcgcgctcg cgctggaaca tcagcgggat
     1801 gcgcttgccg gcattgcccg gcaccatcac gaagcgcgcc tgatccttgc ggaagccggc
     1861 gccgacgtgg gcctggcgct cgccgacggt gaccagcgcc ttgacgtggt tgatgcgcgt
     1921 cgtcatgcgc aggatcaccg gcacctggaa acgctcggac agttcgtagg cttccagcgt
     1981 catcgcgtag gcttcttgcg aatcggccgg ctcgaacacc ggcacgtgcg cgaagcgccc
     2041 ccagtggcgg gaatcctgct cgttctgcga cgacgacagg ccgacgtcgt cggcgatggc
     2101 aatcaccagg ccgccgctga caccggtcag ggtcagcgtc atcagtgcgt cggaggcgac
     2161 gttcatgccg acgtgcttca tgcagcagaa cgaacgcgaa ccggcatagg cggcgccgat
     2221 cgccacttcc agcgagacct tttcattgac cgaccactcg gcgt
//
LOCUS       DAOSDX010000479         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16627_length_2259_cov_3.286298, whole genome shotgun sequence.
ACCESSION   DAOSDX010000479 DAOSDX010000000
VERSION     DAOSDX010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16627_length_2259_cov_3.286298"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLN02_10425"
     CDS             <1..488
                     /locus_tag="PLN02_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HOI52781.1"
                     /translation="THANQWNLSVGLAKEIALNEATTLRPFGQLHYTNVAIDGYREKG
                     GAPALLYKDNTIDSLVSEIGLAAERKLNNGWSVNGSLAWEHEFSNDSRSIRTAFVADP
                     ANTVFRVKGVDGQRDYGRVAVGVSKEIDRDRSFFVNLDGVVGNSDYRDYTLEFKYRQS
                     F"
     gene            394..1365
                     /locus_tag="PLN02_10430"
     CDS             394..1365
                     /locus_tag="PLN02_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HOI52782.1"
                     /translation="MTVPSSSTSMASSVTAITAITRWNSSIAKASDPASMSRLKSTLA
                     GLLPPVFLSFLLVFSVGGLVAGCAGSQARFESLRDSASLRQEVVSAQGFRLLLLERSR
                     LTEQGGHVHVYIEGDGRPWIAGGEVVAADPTPRKPMALELLVQDERAALYLGRPCYFV
                     GVGDAVCSPQWWTSQRYSERVVQAMADALADWLARRPAINSVSLVGYSGGGVLAMLLG
                     ERVPQVRRVVSIVAPLDHERWTALHGYSPLAGSLNVASARHWRPDVVRQAIFGAEDKN
                     VPAVAMLHALPPGTEVLVLPGMAHECCGDQAWTSILGRLNKAAAASP"
     gene            complement(1362..1745)
                     /gene="apaG"
                     /locus_tag="PLN02_10435"
     CDS             complement(1362..1745)
                     /gene="apaG"
                     /locus_tag="PLN02_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOI52783.1"
                     /translation="MSAPDKYRIEIIPTPQFIPEQSDPDDNRYVFAYTIAIRNVGSVP
                     AQLVSRHWIITDANNQVQEVRGLGVVGKQPLLQPGERFEYTSGSSLTTPIGTMKGSYQ
                     MVAEDGTHFDAEIPEFVLAIPRALH"
     gene            1781..>2259
                     /gene="rpe"
                     /locus_tag="PLN02_10440"
     CDS             1781..>2259
                     /gene="rpe"
                     /locus_tag="PLN02_10440"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOI52784.1"
                     /translation="MSARDFVIAPSILSANFAKLGEEVANVIASGADWIHFDVMDNHY
                     VPNLTIGPLVCEAIRPCTDAPIDVHLMVKPVDRIVPDFAKAGANLITFHPEASEHIDR
                     TLSLIRDAGCQAGLVFNPATPLDYMDYVMDKLDVVLLMSVNPGFGGQKFIPGTLAKAR
                     "
BASE COUNT          390 a          666 c          719 g          484 t
ORIGIN      
        1 acacccatgc caaccagtgg aatctttcgg tcggtttggc caaggagatc gcgctgaacg
       61 aagcgaccac gctgagacct ttcggtcagt tgcactacac caatgtcgcg attgacggct
      121 atcgcgaaaa aggaggggcg ccggcgctgc tctacaagga caacacgatc gattccctgg
      181 tttccgagat cggccttgct gccgagcgca agctgaacaa cggttggtcg gtcaatggca
      241 gtctggcttg ggaacacgag ttcagcaacg atagccgctc gatccgtacg gcattcgtcg
      301 ccgatccggc caataccgtc ttccgcgtca agggcgtgga cggccagcgt gactatggcc
      361 gcgttgccgt cggtgtcagc aaggagatcg atcgtgaccg ttccttcttc gtcaacctcg
      421 atggcgtcgt cggtaacagc gattaccgcg attacacgct ggaattcaag tatcgccaaa
      481 gcttctgatc cggcgagcat gtcgcgtttg aaaagtacgc tggcggggtt gctcccgcca
      541 gtttttttat cttttctgct ggtgttttcg gtcggtgggc tggtggccgg ttgtgccggt
      601 tcgcaggccc gcttcgaaag cttgcgtgac agcgcttcct tgcgtcagga agtcgtttcg
      661 gcgcaagggt ttcgcctctt gctgcttgag cgctcaaggc tgaccgagca aggggggcac
      721 gttcatgttt acatcgaagg cgatgggcgt ccctggattg cagggggcga ggtggtcgcc
      781 gccgacccga ccccgcgcaa acccatggcg cttgagctgc ttgttcagga cgagcgcgct
      841 gcgctatatc tggggcgccc ctgttatttc gtcggcgttg gcgatgccgt ctgctcgccg
      901 caatggtgga ccagccagcg ttattcagag cgcgtggttc aggcaatggc tgacgcgctt
      961 gccgactggc tggcgcgtcg accagcgatc aatagtgtca gtctggttgg ctactcgggg
     1021 ggcggtgtcc tcgccatgct gcttggcgag cgtgtgccgc aagtccggcg ggtcgtgtcc
     1081 atcgtcgcac cgctggacca tgagcgttgg acggccttgc acggctattc gccgctcgcc
     1141 ggctcattga atgttgccag tgcgcgacat tggcgcccgg acgttgtgcg gcaggcgata
     1201 ttcggtgccg aagacaagaa tgtccccgcc gtggcaatgc tgcatgcgct accgccgggc
     1261 acggaagtgc tggtcctgcc gggaatggcg cacgaatgct gcggcgatca agcctggaca
     1321 agcatcctgg ggcgcttgaa caaggcagcg gccgcttcgc cttagtgcag cgcgcgcgga
     1381 atcgccagga cgaactcggg aatctcggcg tcgaaatgcg tgccgtcctc ggccaccatc
     1441 tggtagctgc ccttcatcgt gccgatcggt gtcgtcagcg aggagccgct ggtgtattcg
     1501 aaacgctcgc ctggttgcag caatggttgc ttaccgacga cgccgaggcc gcgcacttcc
     1561 tgcacctgat tgttggcgtc ggtgatgatc cagtgacgcg acaccagttg cgccggcacg
     1621 ctgccgacgt tgcggatggc gatcgtgtag gcgaagacgt agcggttgtc gtcggggtcg
     1681 gactgctcgg gaatgaactg cggggtaggg atgatttcga tgcggtactt gtcgggggcg
     1741 ctcatgggat aatttggtct cttgttttca gggaaacatc atgtcagcca gggatttcgt
     1801 cattgcaccg agcattctgt ccgccaattt cgccaaactg ggcgaggaag tggccaacgt
     1861 catcgcctct ggcgccgact ggattcactt cgacgtgatg gacaaccatt atgttcccaa
     1921 cctgaccatc ggaccactgg tctgcgaggc gatccgcccc tgtaccgacg cgccgatcga
     1981 cgtgcatctg atggtcaagc cggtcgatcg catcgtgcct gatttcgcca aggccggcgc
     2041 caacctcatc accttccacc cggaagcctc cgagcacatc gaccgcacgc tgtcgctgat
     2101 ccgcgatgcc ggctgtcagg ctggtctggt cttcaacccg gcaacgccgc tcgattacat
     2161 ggattacgtc atggacaagc tggacgtcgt cctgctgatg agcgtcaacc ccggcttcgg
     2221 cggccagaag ttcatccccg gcacgctggc caaggcgcg
//
LOCUS       DAOSDX010000480         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16628_length_2259_cov_3.235481, whole genome shotgun sequence.
ACCESSION   DAOSDX010000480 DAOSDX010000000
VERSION     DAOSDX010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16628_length_2259_cov_3.235481"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="PLN02_10445"
     CDS             complement(<1..1935)
                     /locus_tag="PLN02_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOI52785.1"
                     /translation="MSFLDNVSFGLRRKLPLILQTEATECALACIAMVAGYHGLRTDL
                     ATLRRIFLVSIKGSTLGHLMQIAHSLKLTTRPVKLELEDLPQLRLPCILHWNFNHFVV
                     LKAVERNTVTIHDPAFGLRKLSLDEVSGQFTGVALELWPNPEFKPLSFRQRIRLRDLL
                     GHVTGLFRSLGQILLLAVSLEIFALVSPFFLQWVIDNVVVSGDRDLLTTLALGFGLLM
                     LLQQGIGTLRSWVIMHMSTTLNLQWRANIFSHLLRLPVDYFEKRHLGDVVSRFGSIDV
                     IQKTLTTAFVEGILDGLMTIVTLVMMFIYSPSLAWIAVSAMALYGIVRWLWYRPLRNA
                     TEEQIVHAAKQQSHFLETVRGVRSIKLFQRQEERRSTWLSLLVDQINADLRNQKLQIF
                     YRLINGILFGVERILVIWLGARLILDGNFTVGALMAFSAYKDQFDSRVSALIDKLVDL
                     KMLQLQGERLADIVLADAEPSAPPLPATARQATPEASIELRGLRYRYADGEPWVLDGI
                     DLRIAPGESIAIVGPSGCGKSTLISAMLGIRTPNEGEVLVGGASVAQVGFEALRGMVG
                     TVLQDDVLFAGSIADNISFFDPRPDFARVEECAKLAAIHDDIQAMPMAYNTLIGDMGA
                     ALSGGQKQRLSIARIFLKNPP"
     gene            complement(1964..>2259)
                     /locus_tag="PLN02_10450"
     CDS             complement(1964..>2259)
                     /locus_tag="PLN02_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52786.1"
                     /translation="QAYPYQKFGHARGTVVSVARTALPGSEISSLLAPGAEAQGSEPL
                     YLISVALEQQSINAYGVAQPLQAGMLLEADVLQESRKLYEWVLEPLFSLSGKL"
BASE COUNT          391 a          658 c          838 g          372 t
ORIGIN      
        1 cggcgggttc ttgaggaaga tgcgggcgat cgagagccgc tgcttctggc cgccggagag
       61 ggcagcgccc atgtcgccga tcagcgtgtt gtaggccatc ggcatcgcct ggatgtcgtc
      121 gtggatcgcc gccagcttcg cgcattcctc gacccgcgcg aaatccggac gagggtcgaa
      181 gaaactgatg ttgtcggcga tcgaaccggc gaacaggacg tcgtcctgga gcacggtgcc
      241 gaccatgccg cgcagcgcct cgaagccgac ctgggcgacg ctggcgccgc cgaccaggac
      301 ttcgccctcg ttcggcgtgc ggatgccgag catggcgctg atcagcgtcg acttgccgca
      361 gccggacggg ccgacgatgg cgatcgattc gccgggcgcg atgcgcaggt cgatgccgtc
      421 gagcacccag ggctcgccgt cggcgtagcg gtagcgcagg ccgcgcagtt cgatcgaggc
      481 ttccggcgtt gcttgccgcg ccgtcgccgg cagcggcggc gcgcttggct cggcgtcggc
      541 gaggacgatg tcggccaggc gctcgccctg cagttgcagc atcttgaggt cgaccagctt
      601 gtcgatcagg gcgctgaccc gcgaatcgaa ctggtccttg taggcggaaa acgccatcag
      661 cgcgccgacc gtgaagttgc cgtcgaggat cagcctggcg ccgagccaga tgaccaggat
      721 gcgctcgacg ccgaacagga tgccgttgat caggcggtag aagatctgca gcttctggtt
      781 gcgcaggtcg gcgttgatct ggtcgaccag gaggctgagc caggtcgagc ggcgctcctc
      841 ctggcgctgg aacagcttga tgctgcgcac gccgcgcacc gtttcgagga aatggctctg
      901 ctgcttggcg gcatgcacga tctgttcttc ggtggcgttg cgcagcggcc ggtaccagag
      961 ccagcggacg atcccgtaga gcgccatggc gctgaccgcg atccacgcca gcgaggggct
     1021 gtagatgaac atcatgacca gcgtgacgat ggtcatcagg ccgtcgagga tgccttcgac
     1081 gaaggcggtg gtcagcgtct tctggatgac gtcgatcgaa ccgaagcggg agacgacgtc
     1141 gccgaggtgg cgtttctcga agtaatcgac cggcaggcgc agcaggtggc tgaagatgtt
     1201 ggcccgccat tgcaggttga gcgtcgtcga catatgcatg atgacccacg agcgcagcgt
     1261 gccgatgccc tgctggagca gcatcagcag gccgaagccg agggcgagcg tggtcagcag
     1321 gtcgcggtcg ccggagacaa cgacgttgtc gatcacccac tgcaggaaga aggggctgac
     1381 cagggcgaag atttccagcg agacggcgag cagcaggatc tggccgagcg agcggaacag
     1441 cccggtgacg tggccgagca ggtcgcgcag gcggatgcgc tggcggaagc tgagcggctt
     1501 gaactcgggg ttcggccaga gttccagggc aaccccggtg aactggccgg aaacctcgtc
     1561 gaggctcagc ttgcgcaggc cgaaggcggg gtcgtggatg gtcaccgtgt tgcgctcgac
     1621 cgccttgagt acgacgaagt ggttgaagtt ccagtgcagg atgcagggca ggcgcagttg
     1681 cggcaggtct tcgagttcca gtttgaccgg tcgcgtcgtc agcttcagcg agtgggcgat
     1741 ctgcatcagg tggccgagcg tgctgccctt gatcgagacc aggaagatgc ggcgcagggt
     1801 ggcgaggtcg gtgcgcaagc cgtggtagcc ggcgaccatg gcgatgcagg cgagggcgca
     1861 ttcggtcgcc tcggtctgca ggatcagcgg cagtttgcgg cgcaggccga aggaaacgtt
     1921 gtcgaggaag gacatggggg accggaagtg aggtgacgga cgatcagagc ttgccgctca
     1981 ggctgaacag gggttcgagc acccactcgt agagcttgcg gctttcctgg aggacatcgg
     2041 cttcgagcag catgcccgcc tgcagcggct gggcgacgcc gtaggcattg atgctctgct
     2101 gttcgagggc gacgctgatc aggtagagcg gctcgctgcc ctgagcctcg gcgcccggcg
     2161 ccagcaggct ggaaatctcg ctgccgggca gcgcggtgcg cgccaccgaa acgacggtgc
     2221 cgcgggcgtg gccgaacttc tggtagggat aggcctggt
//
LOCUS       DAOSDX010000481         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16699_length_2251_cov_3.125683, whole genome shotgun sequence.
ACCESSION   DAOSDX010000481 DAOSDX010000000
VERSION     DAOSDX010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16699_length_2251_cov_3.125683"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="PLN02_10455"
     CDS             <1..1169
                     /locus_tag="PLN02_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HOI52787.1"
                     /translation="KVLVNPGWLAVHGKEGQSGDEGNLVAVDKDEKVKTEEVTVKASA
                     TKPPPRYSEATLLSAMEGAGKMVDDEELKAAMAGRGLGTPATRAQIIENLIGEQYMLR
                     EGRELIPTAKAFNLMTLLNGLGVEELTQPELTGDWEWKLGRIEKGEFTRDEFMREIAE
                     MTRHIVERAKSYDSDTIPGDFGVLKAPCPKCGGVIRETYKKFQCSGCDYSLWKIVAGR
                     QYELAEVETLLTEGQVGPLTGFRNKMGRSFAAAIKLNAEHLPEFDFGQDKADEASAEP
                     VDFSGQESLGKCPKCAANVFDHGSSYVCEKSVGPDKTCDFRSGKIILQQPVEPVQMKK
                     MLETGKTDLLKEFVSNRTKRKFSAYLAVNEGKVGFEFEKKAPSAKAPAKKKAAG"
     gene            complement(1170..>2251)
                     /locus_tag="PLN02_10460"
     CDS             complement(1170..>2251)
                     /locus_tag="PLN02_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52788.1"
                     /translation="SHVLRQRLDSPELLDKLSRITSSADHLLGIINDLLDFSKIEAGR
                     LQLEAKPLHPLQVLDSVVSMITEQAQVKGLEVNYRCADFPETLRGDAGRLIQALLNLA
                     GNAVKFTREGEVELLVERVAEDVDGVLVRFSVRDTGIGIPPEVIEKLFSPFQQADDST
                     ARRYGGTGLGLAITRRLARLMGGDAGVDSVPGAGSRFWFTAHLQREGQNGGHYLPERV
                     PGEHPERLLQEAFAASRILLVEDDPINQEVAAELLENVGLIVELAADGQVAVDKVSQA
                     AHPFALILMDLQMPRLGGLEAMVKLRELPGFATPVIAMTANAFAEDQARCRAAGMVDF
                     IAKPVDPILLYETLLEWLRRGRQAG"
BASE COUNT          473 a          728 c          704 g          346 t
ORIGIN      
        1 gcaaggtcct ggtcaacccg ggctggctcg ccgtccatgg caaggaaggc cagagcggcg
       61 acgaaggcaa cttggtcgcc gtcgacaagg acgaaaaggt caagaccgag gaagtcacgg
      121 tcaaggccag cgccaccaaa ccgccgccgc gttattcgga agccaccctg ttgtcggcca
      181 tggaaggcgc cggcaagatg gtcgacgacg aggaactgaa ggcggcaatg gctggccgcg
      241 gcctcggcac gccggcaacg cgcgcccaga tcatcgagaa cctgatcggc gaacagtaca
      301 tgctgcgcga aggccgcgaa ctgatcccga cggccaaggc cttcaacctg atgacgctgt
      361 tgaacggtct cggtgtcgag gaactgaccc agccggaact caccggcgac tgggaatgga
      421 agctcggccg catcgaaaag ggtgaattca cgcgcgacga gttcatgcgc gagatcgccg
      481 aaatgacccg tcacatcgtc gagcgtgcca agtcttacga cagcgacacc atccccggcg
      541 actttggcgt tctgaaggcg ccctgcccga aatgcggcgg cgtcatccgc gagacttaca
      601 agaaattcca gtgcagcggc tgcgactatt cgctgtggaa gatcgtcgcc ggccgtcagt
      661 acgaactggc cgaagtcgaa accctgctca ccgaaggcca ggtcggcccg ctgaccggtt
      721 tccgcaacaa gatgggccgc agcttcgcgg cagcgatcaa gctcaacgcc gaacacctgc
      781 cggagttcga tttcggccag gacaaggccg acgaagccag cgccgagcca gtcgattttt
      841 cgggccagga aagcctgggg aaatgcccga agtgcgccgc caacgtgttc gatcacggca
      901 gcagctatgt ctgcgagaaa tcggtcggcc ctgacaagac ctgcgatttc cgatccggca
      961 agatcatcct gcagcaaccg gtcgaaccgg tgcagatgaa gaagatgctg gaaaccggca
     1021 agaccgacct gttgaaggaa ttcgtctcca accggacgaa gcgcaagttc tcggcctatc
     1081 tggcggtcaa cgaaggcaag gtcggtttcg agttcgagaa gaaggctccg tcggccaagg
     1141 cacctgcgaa gaagaaggcc gccggctgat cagccggcct ggcgcccgcg gcgcagccat
     1201 tcgagcaggg tttcgtagag caggatggga tcgaccggct tggcgatgaa atcgaccatg
     1261 ccggcagcgc gacagcgcgc ctgatcctcg gcgaaggcgt tcgccgtcat ggcgatcacc
     1321 ggcgtcgcaa agcccggcag ctcgcgcagt ttcaccatcg cctccagccc gcccagcctg
     1381 ggcatctgca ggtccatcag gatcaaggcg aacggatgcg ccgcctggct gaccttgtcc
     1441 acggccacct gcccatcggc cgccagttcg acgatcagcc cgacgttttc gagcaactcc
     1501 gccgccacct cctggttgat cgggtcatcc tcgaccagca ggatacgcga agcggcgaaa
     1561 gcctcctgca acagccgctc ggggtgctcg ccaggcaccc tttccggcag gtaatggccc
     1621 ccgttctgcc cttcccgttg cagatgggcg gtgaaccaga agcgactccc cgcccccggc
     1681 acactgtcga cgccggcgtc gccccccatc agccgggcca gccgccgggt gatcgccagc
     1741 ccgaggccgg tgccaccgta acgccgggcc gtggaatcgt cggcctgctg gaaaggcgag
     1801 aacagctttt cgatcacttc cggcggaatg ccgatcccgg tatcgcggac cgaaaaccga
     1861 accaggacgc catcgacgtc ttccgccacg cgctcgacca gcaattcgac ctcgccctcg
     1921 cgggtgaact tgacagcgtt gccagccagg ttgagtagcg cctggatcag acgcccggca
     1981 tcgccacgca gggtttccgg gaagtcggcg caacggtagt tcacttccag ccccttgacc
     2041 tgcgcctgct cggtgatcat cgacacgacg ctgtcgagca cctgcagggg atgcaagggc
     2101 ttggcctcaa gctgcaggcg accggcctcg atcttcgaga aatcgagcag gtcgttgatg
     2161 atgccgagca agtgatcggc cgaagaggta atgcgggaaa gcttgtcgag cagttccggg
     2221 gaatcgagcc tctggcgaag cacatgcgac a
//
LOCUS       DAOSDX010000482         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16728_length_2248_cov_3.796626, whole genome shotgun sequence.
ACCESSION   DAOSDX010000482 DAOSDX010000000
VERSION     DAOSDX010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16728_length_2248_cov_3.796626"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..432
                     /locus_tag="PLN02_10465"
     CDS             148..432
                     /locus_tag="PLN02_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HOI52789.1"
                     /translation="MKLISNKALRDFAIRHPDADAPLQNFRGLVEKGRFASYAELRLT
                     FGSVDKVGERYVFNIGGNKYRLIAAIAFAPQLFWIKAVLTHAEYDKGGWK"
     gene            429..839
                     /locus_tag="PLN02_10470"
     CDS             429..839
                     /locus_tag="PLN02_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI52790.1"
                     /translation="MSVDELLAPWQTVNAALGLSGPVRDEAHHAQLLAFVDEAFARFG
                     NDDQHPIFTLVALVADRIREYEDRVHPWPDNSTPASRLAFLMESHGLNQKDLPEVATQ
                     SVISEILSGKRRINLRQAAALAKRFGIPLDLFVD"
     gene            complement(859..1617)
                     /locus_tag="PLN02_10475"
     CDS             complement(859..1617)
                     /locus_tag="PLN02_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HOI52791.1"
                     /translation="MIPLLRHSARPSRPRRLTLQILRYNPATPEIAPYLQDFAIDESD
                     GMTLFIALNEIRASQDPSLQFDFVCRAGICGSCAMLVDGRPALACRTLTRDCGPRIVL
                     APLPFFELIGDLSVNTGKWMRGTAERLQTWIAQTQPVDLDALEARMEPALAEAIYELD
                     RCIECGCCIAACGTAQMREDFVGAVGLNKIARFRLDPRDTRSDADFYELIGDDNGVFG
                     CMSLLGCHDFCPKQLPLQTQIAFLRRKMAVEGWR"
     gene            complement(1614..>2248)
                     /locus_tag="PLN02_10480"
     CDS             complement(1614..>2248)
                     /locus_tag="PLN02_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287528.1"
                     /note="part of three member fumarate reductase enzyme
                     complex FrdABC which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdC is the
                     cytochrome b-556 subunit; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HOI52792.1"
                     /translation="AQAEFAGIAESRGKENAALLLREMQATMTARVGIVRHGDTLHTA
                     VDALQGLLRRSLDLGLRSSRPGANPELTQAWRLRKMLKLALCVAWGALQRTESRGAHY
                     REDFPRRNDADWLKRTLATWPDPELTLPALAYEALDVTRMELPPGWRGYGARDAIEHP
                     LTTARQAEVDAIRARLAGADRHTVQDALLPFRDLLPPHLCGANARLGDPA"
BASE COUNT          369 a          654 c          814 g          411 t
ORIGIN      
        1 gagcctgtca tgggatggaa ggggctatca tgttactgcg caatgacgaa tgggttatcg
       61 gggcgggcgg caggtgagca ctggcaagaa gggctgaatg tcggccatcg caatttgcaa
      121 taaatcgcag tttgctataa attgagcatg aagctgatat cgaacaaggc actgcgggat
      181 ttcgcgatca ggcatccgga cgccgatgcc ccgctgcaga atttccgtgg cttggtggaa
      241 aaaggccgtt ttgccagcta cgccgagctg agactgacgt tcggcagtgt ggataaggtc
      301 ggcgaacgtt acgtgttcaa tatcggtggc aacaaatatc gcttgatcgc cgccattgcc
      361 ttcgcgccgc aactgttctg gatcaaggct gtgctgaccc atgccgagta cgacaaagga
      421 ggctggaaat gagtgttgat gagttgctgg ccccctggca gacggtcaat gcagccctcg
      481 gtttgtccgg tccggtgcgc gacgaagcgc atcatgcgca gttgctggca tttgtggacg
      541 aggcgtttgc acggtttggt aatgacgatc aacacccgat tttcacgctg gttgccctgg
      601 ttgccgaccg catccgtgaa tacgaagacc gcgtgcatcc ttggccggat aactccacac
      661 cggccagccg tctggctttc ctgatggaaa gccatgggct gaatcagaag gacctgcccg
      721 aggtggcaac gcagtcggtg atttccgaaa tcctgtcggg caagcgtcgg atcaatcttc
      781 gtcaggccgc tgctttggcc aaacgcttcg gtattccgct cgacctgttt gtcgactgaa
      841 agtcggtagg gctggcgctc agcgccagcc ttcaaccgcc atcttgcggc gcaggaaggc
      901 gatctgcgtc tgcagcggca actgcttggg acagaagtcg tggcagccga gcaggctcat
      961 gcagccgaag acgccgttgt cgtcgccgat caactcgtag aaatcggcgt cgctgcgcgt
     1021 gtcgcgcggg tcgaggcgga agcgggcgat cttgttgagg ccgacggcgc cgacgaagtc
     1081 ctcgcgcatc tgcgccgtgc cgcaggcggc gatgcagcag ccgcattcga tgcagcggtc
     1141 gagttcgtag atcgcttcgg caagcgccgg ttccatgcgc gcttcgaggg cgtcgaggtc
     1201 gaccggctgt gtctgggcga tccaggtctg cagacgttcg gcggtgccgc gcatccactt
     1261 gccggtgttt accgaaaggt cgccgatcag ttcgaagaag ggcagcggcg ccagcacgat
     1321 gcgcgggccg cagtcgcggg tcagggtgcg gcaggcgagc gccggccggc cgtcgaccag
     1381 catcgcgcaa ctgccgcaga tgccggcgcg gcagacgaag tcgaactgca gcgaggggtc
     1441 ctggctggcg cggatttcgt tgagcgcgat gaacagcgtc atgccgtcgc tttcgtcgat
     1501 cgcgaaatcc tgcagatagg gcgcgatttc cggcgtcgcc gggttatagc gcaggatctg
     1561 cagggtcagg cggcgcggcc gcgacgggcg ggcggagtgg cgcagcagcg ggatcatgcg
     1621 gggtctccga ggcgggcgtt ggcgccgcac aggtgtggcg gcagcaggtc gcggaagggt
     1681 agcagggcat cctgcacggt gtggcggtcg gcgccggcga ggcgggcgcg gatggcgtcg
     1741 acttcagctt ggcgggcggt ggtcagcggg tgttcgatgg cgtcgcgggc gccgtagccg
     1801 cgccagccgg gcggcagttc catgcgcgtg acgtcgagcg cctcgtaggc gagcgccggc
     1861 agcgtcagct cggggtcggg ccaggtggcc agcgtgcgtt tcagccagtc ggcgtcgttg
     1921 cgccgcggaa agtcctcgcg gtagtgggcg ccgcggcttt cggtgcgctg cagggcgccc
     1981 caggcgacgc acagcgccag cttgagcatc ttccgcaggc gccaggcctg cgtcagctcc
     2041 gggttggcgc ccggccggct gctgcgcagg ccgaggtcca ggctgcgccg cagcaggccc
     2101 tgcagggcgt cgaccgcggt gtgcagcgta tcgccgtgac ggacgatgcc gacgcgggcg
     2161 gtcatcgtcg cctgcatctc gcgcagcagc agggcggcgt tttccttgcc gcggctttcg
     2221 gcgatgccgg cgaattcggc ctgcgccc
//
LOCUS       DAOSDX010000483         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16782_length_2242_cov_3.174211, whole genome shotgun sequence.
ACCESSION   DAOSDX010000483 DAOSDX010000000
VERSION     DAOSDX010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16782_length_2242_cov_3.174211"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /gene="aroE"
                     /locus_tag="PLN02_10485"
     CDS             complement(<1..690)
                     /gene="aroE"
                     /locus_tag="PLN02_10485"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOI52793.1"
                     /translation="MPDLYAVFGNPIAHSKSPFIHAAFAAQTGADLVYQARLAPVDDF
                     AGALRAFVAEGGRGANVTVPFKEEAFRLCDELTPRAARAGAVNTLSFADGRVRGDNTD
                     GAGLVADTEGNLGVVLAGKRVLLLGAGGAARGVIEPLLVAGVGELAVANRSADKALVL
                     AAAFADLAPVGGGGFGDLVGRRFDVVINATSASLAGDRLPLPEGLFAAGALAYDMMYG
                     KGETPFLAQARE"
     gene            complement(699..1586)
                     /locus_tag="PLN02_10490"
     CDS             complement(699..1586)
                     /locus_tag="PLN02_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOI52794.1"
                     /translation="MTRQIRPLTGTPSDRRLWLALGVSLLLHGLLLSLHFAFPEASRV
                     AREKALDIILVNARAQRPPADAQALAQANLDGGGNTDENLRAKTPLPATEQQSSGEDM
                     QQMRRRVRELEAAQHKMLTQAANLARVAAAETRSEQPEPSPQLTGTDLADTARAMARL
                     EGEIAKSIEEYNKRPQKKFIGARTKEYRLAPYFDAWKQKIERIGTLNYPEAARGKLYG
                     KVVLFVELRVDDGSLYNVEISRSSGHKLLDQAALRILRMAAPFGPIPREAMDGATVLS
                     FAREWNFVPGDTLATRNSN"
     gene            complement(1583..>2242)
                     /locus_tag="PLN02_10495"
     CDS             complement(1583..>2242)
                     /locus_tag="PLN02_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOI52795.1"
                     /translation="DLADPESCTVDISERKRGSPLDKLVAELMIVANSTWGGLLAERN
                     IACLYRAQTQGKVRMTTSPLPHEGLGVPQYAWMTSPLRRYCDLVNQWQLIACLRETPP
                     PFPQKSAELFAAMRDFELTYAAYAEFQRLMERYWCLRWLQQHGLVEIDATLRREQTVK
                     LDHLPLLARVASVPADLQPGQRLHLAVESIDLLQPEVALRFVALLDADEPACDLEEDV
                     A"
BASE COUNT          366 a          728 c          786 g          362 t
ORIGIN      
        1 ttcccgggcc tgcgccagga aaggcgtctc gcccttgccg tacatcatgt cgtaagccag
       61 cgcaccggcg gcgaaaagtc cttccggcaa gggcaggcgg tcaccggcca gactggccga
      121 ggtggcgttg atgacgacat cgaagcgacg cccgaccagg tcgccgaaac cgccgccgcc
      181 gaccggtgcc agatcggcga atgccgccgc cagcacgagc gccttgtcgg cgctgcggtt
      241 ggcgaccgcc agttcgccga caccggccac caacaacggt tcgatgacgc cgcgcgcggc
      301 gccgccggcg ccgagcagca gcacgcgctt gccggccagg acgacgccga gattgccctc
      361 ggtgtcggcg accagaccgg cgccatcagt gttgtcgccg cgcacacgac cgtcggcgaa
      421 actcagcgta ttgaccgcgc cggcgcgcgc cgcccttggc gtcaactcgt cgcacaggcg
      481 gaaggcttct tccttgaacg gcaccgtgac attggcacca cggccaccct cggcaacgaa
      541 ggcgcgcaag gcgccggcaa aatcatcgac gggcgccaga cgcgcctgat agaccaggtc
      601 ggcgccggtc tgcgcagcga aggcggcgtg aatgaaaggc gatttcgagt gggcgatcgg
      661 gttgccgaaa acggcgtaga ggtcaggcat gggcaggctt aattgctgtt gcgggtcgcc
      721 agggtgtcgc cggggacgaa attccattcc cgggcaaacg acagcacggt ggcgccgtcc
      781 atcgcctcgc gcggaatcgg cccgaacggc gccgccatgc gcaggatgcg cagggccgcc
      841 tggtcaagca gtttgtgtcc ggacgaacgg gaaatctcga cgttgtacag gctgccgtcg
      901 tcaacgcgca attcgacgaa gaggaccacc ttgccgtaga gcttgccgcg cgccgcttcc
      961 gggtaattga gcgtgccgat gcgctcgatc ttctgcttcc aggcgtcgaa atacggcgcc
     1021 aggcggtatt ccttggtgcg cgcgccgatg aatttctttt gcggccgctt gttgtattcc
     1081 tcgatcgact tggcaatctc gccttccagg cgggccatcg cccgtgccgt atcggcgaga
     1141 tcggtaccgg tcaactgcgg cgacggctcg ggttgttcgc tgcgcgtttc ggcagccgcg
     1201 acgcgcgcga gattggccgc ctgggtcagc atcttgtgtt gcgccgcctc gagttcgcgc
     1261 acgcggcgcc gcatctgctg catgtcctcg ccgctggact gctgttcggt cgccggcagc
     1321 ggcgtcttgg cgcgcaggtt ctcgtcggtg ttgccgccac cgtcgagatt ggcctgcgcc
     1381 aaggcctggg catcggccgg cgggcgctgg gcgcgggcgt tgaccagaat gatgtcgagc
     1441 gccttctcgc gggcgacccg cgaagcttcc gggaaggcga aatgcagact gagcagcagg
     1501 ccgtgcagca acagggaaac gccaagcgcc agccacaggc gacgatcgct cggcgtgccg
     1561 gtcagcgggc ggatctggcg cgtcacgcga cgtcctcctc caggtcgcag gcgggctcgt
     1621 cagcgtcgag cagggcgacg aagcgcaggg cgacttcggg ctgcagcagg tcgatgctct
     1681 cgaccgccag atgcaggcgc tgaccgggct gcaggtcggc gggcaccgaa gcgacgcgcg
     1741 ccagcagcgg cagatgatcg agcttgaccg tctgctcgcg gcgcagggtg gcgtcgattt
     1801 cgacgaggcc gtgctgctgc agccagcgca gacaccagta gcgctccatc aggcgctgga
     1861 attcggcgta ggcggcgtag gtcaactcga aatcgcgcat cgccgcgaac agctcggccg
     1921 acttctgcgg gaagggtggc ggtgtttccc gcagacaggc gatcaactgc cattgattga
     1981 ccaggtcgca gtagcggcgc agcggcgagg tcatccaggc atactgcggc acgcccaggc
     2041 cttcgtgcgg cagcggcgaa gtggtcatgc gcaccttgcc ctgggtctgg gcgcggtaca
     2101 ggcaggcgat gttgcgctcg gcgagcaggc cgccccaggt cgaattggcg acgatcatca
     2161 gctcggcgac cagcttgtcg agcgggctgc cgcgtttgcg ttcgctgatg tctacagtgc
     2221 agctttccgg gtcggcgaga tc
//
LOCUS       DAOSDX010000484         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16811_length_2239_cov_2.997253, whole genome shotgun sequence.
ACCESSION   DAOSDX010000484 DAOSDX010000000
VERSION     DAOSDX010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16811_length_2239_cov_2.997253"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PLN02_10500"
     CDS             complement(<1..642)
                     /locus_tag="PLN02_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HOI52796.1"
                     /translation="MSPSPSPLRLNDLTHPVVAALISVIVNYGGTFILVFQAAQVAGL
                     SPAQTASWVWSISIGVGVTGIILSWLAREPIITAWSTPAAAFLVTALAVTPYAEAVGA
                     YLISAAAFVALGLSGYFEKVIRLIPQGVASGLLAGILLQFGTGAFGGLSVDPLLAGLL
                     IAAYLVFKRFSARYAVVGILVLGLVFLLAQGRVDLSGLSLKLAAPVFTMPAFSL"
     gene            751..2229
                     /locus_tag="PLN02_10505"
     CDS             751..2229
                     /locus_tag="PLN02_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI52797.1"
                     /translation="MFRHAQLESVKAWLGDPAHQALPRHVRIQRAIRQLILDGALDAG
                     RPLPASRALARSLGVSRDTVEAAYGQLHAEGFIERRVGSGSFVSAQALRVPRRPPSLR
                     PGQPTAALSRRGNAMLAGGGVRESLTARPFAPGIPETRSFPLAVWERLQRQVLKEHGA
                     RALAHCHPQGMESLRRAIADYINLERGARAAPERILVLTSSQQALSLCATVLLDVGER
                     ICVEDPVYHGARKAFDAAGLDCVPIPLDGDGMQVGHLDAVANARAVFLTPSHQFPSGT
                     TLSLARRLAVIDWARRQQAWIIEDDYDSEFHYAGKPTACVQGLDDEQRTIYIGTFTKS
                     LFPGLRIAYMLLPPALVAPMTVARSLQDGHNATIAQLTLARFIEGGHFGAHVRAMRSV
                     YAARRDRLARLVEEHLGDFVTAAEPAGGMQMPCFLHAGIDETQALTAAQRAGVELLGL
                     SPLSAGGGGRPGFLMGFAAYTPEEMAIALRQLAAALRVLTAK"
BASE COUNT          383 a          764 c          780 g          312 t
ORIGIN      
        1 gagcgagaac gcgggcatgg tgaacaccgg cgcggccagt ttcaggctca gtcccgacag
       61 atcgacgcgg ccctgcgcca gcaggaatac caatcccagc accaggatgc ccaccaccgc
      121 atagcgcgcg gaaaagcgct tgaacaccag ataggcggcg atcagcagtc ccgccagcag
      181 cggatcgacg ctcaagccgc cgaaggcgcc agtgccgaat tgcagcagga tgccggcgag
      241 caggccggag gcgacgccct ggggaatcag ccggatcacc ttctcgaagt aacccgacaa
      301 cccgagggcg acgaaggcgg cggccgagat caggtaggcg ccgaccgctt cggcgtaggg
      361 ggtgactgcc agcgcagtga ccaggaaggc ggcggccggc gtggaccagg cggtgatgat
      421 cggctcgcgg gccagccagc tcaggatgat gccggtgacg ccgacaccga tcgagatcga
      481 ccacacccac gacgccgtct gcgccggact gaggccggcc acctgggcgg cctggaagac
      541 caggatgaag gtgccgccgt aattgacgat gaccgagatc agggcggcga cgacggggtg
      601 ggtaaggtcg ttgaggcgta gtggcgaggg cgatggactc atgggacggc tccggaaaaa
      661 aggggatcga cgatccgcct ggaaagtccc agttatagcg ctagaatggc ctggcaaatc
      721 ccgccaatta atcactggat acccagccaa ttgttccgcc acgcccaact cgaatcggtc
      781 aaagcctggc tcggcgaccc cgcccaccag gccctgcctc ggcatgtgcg catccagcgg
      841 gcgatccgcc agttgatcct cgacggggcc ctcgatgccg gacggcccct gccggcctcg
      901 cgggcactgg cccgatcgct cggggtgtcg cgcgacacgg tggaagcggc ctacggccag
      961 ttgcacgcgg aaggtttcat cgagcgccgg gtcggcagtg gcagtttcgt ttcagcgcag
     1021 gcgctgcggg tgccgcggcg gccgccgtca ttgcggccgg gccagccgac ggcggcgctc
     1081 agccggcgcg gcaacgccat gctggctggg ggcggcgtgc gcgaatcgct gaccgcccgg
     1141 cccttcgccc cgggcatccc ggaaacccgg agctttccgc tggcggtctg ggagcgcctg
     1201 cagcgccagg tgctcaagga gcacggcgcg cgcgccctgg cccattgcca cccgcagggc
     1261 atggagagcc tgcgccgggc gattgccgac tacatcaacc tggaacgggg cgcccgcgcc
     1321 gcgcccgagc gcatcctggt gctgaccagc tcgcagcagg ccctgagcct gtgcgccacg
     1381 gtgctgctcg atgtcggcga gcgcatctgc gtcgaggacc cggtctatca cggcgcccgc
     1441 aaggctttcg acgcagccgg tctggactgc gtgccgattc cgctggacgg cgacggcatg
     1501 caggtcggcc acctggacgc ggtggcgaac gcccgggcgg tgttcctgac tccctcgcac
     1561 cagtttccca gcggcacgac gctgagcctg gcgcggcgtc tcgccgtcat cgactgggcg
     1621 cgccggcagc aggcctggat catcgaggac gactacgaca gcgaattcca ctacgccggc
     1681 aagccgaccg cctgcgtcca ggggctggac gacgagcagc gcacgatcta catcggcacc
     1741 ttcaccaaat ccctgtttcc cggcctgcgc atcgcctaca tgttgctgcc gccggcgctg
     1801 gtggcgccga tgacggtggc gcgcagcctg caggacggcc acaatgcgac gatcgcccag
     1861 ctgaccctgg cgcgtttcat cgaaggcggg catttcggcg cccacgtgcg cgccatgcgc
     1921 agcgtctatg cggcccgccg ggatcggctg gcgcgtctgg tcgaggaaca tctgggcgat
     1981 ttcgtgacgg cggccgagcc ggccggcggc atgcagatgc cctgcttcct gcacgccggc
     2041 atcgacgaga cgcaggcgct caccgccgcc cagcgggcgg gcgtcgaact gctcgggctg
     2101 agtccgttat ccgctggtgg cggcggccgc cccggctttc tgatgggctt cgctgcctac
     2161 acgccggagg aaatggcaat agcgctcagg caactggctg cagctctccg ggtgctgacg
     2221 gcaaaataaa aaacccgcc
//
LOCUS       DAOSDX010000485         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16831_length_2237_cov_2.598992, whole genome shotgun sequence.
ACCESSION   DAOSDX010000485 DAOSDX010000000
VERSION     DAOSDX010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16831_length_2237_cov_2.598992"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="rfbB"
                     /locus_tag="PLN02_10510"
     CDS             complement(<1..752)
                     /gene="rfbB"
                     /locus_tag="PLN02_10510"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOI52798.1"
                     /translation="MILVTGGAGFIGSNFVLDWLAQSAEPVLNLDALTYAGNLENLAA
                     LRGDPRHLFVHGDIGDFDLVAKLLAEHRPRAVVNFAAESHVDRSIHGPEDFIQTNIVG
                     TFRLLEAVRAHCGGLGAEEKAAFRFLHVSTDEVYGSLASDDPAFTETNRYEPNSPYSA
                     SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVIHNALAGKPLPIYGDG
                     RQIRDWLYVKDHCCAIRRVLDAGRLGETYNVGG"
     gene            complement(799..1632)
                     /locus_tag="PLN02_10515"
     CDS             complement(799..1632)
                     /locus_tag="PLN02_10515"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HOI52799.1"
                     /translation="MRRLRALLCGLALLLALPLAAAPPELLLANVYRDDIDVSAYLVS
                     EKLDGVRAVWDGETLRFRSGNRVNAPAWFTDALPKTPMDGELWLGRGQFERLSGIVRR
                     EQPVDAEWREVRYMIFELPGAPGTFAERAGRIRAIVAAAALPWLAEVPQATLASREAL
                     RQRLAQTIKAGGEGLMLHRADAPYETGRSDTLLKLKPWLDAEAVVVAHLPGKGKYAGQ
                     LGALRVRLADGREFRLGTGFTDAQRKSPPPIGSVVTYRYRELTAKGLPRFASFLRRRQ
                     E"
     gene            complement(1635..>2237)
                     /locus_tag="PLN02_10520"
     CDS             complement(1635..>2237)
                     /locus_tag="PLN02_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOI52800.1"
                     /translation="GPNTIVTDGGSTKGDVVAAARAAFRGHIGKFVPAHPIAGAENSG
                     PSAARQDLYQGRKVVVTPLPENNDERLDRVKRAWALCGADIYELTPEMHDRVFAAVSH
                     LPHLLSFALVHDLAVRDDADLFFTFAASGFRDFTRIAASHPEMWRDICLANREALLGE
                     LDSYRAQLDELRDALARADGERLEDVFGIARQARREWAGS"
BASE COUNT          361 a          707 c          814 g          355 t
ORIGIN      
        1 ccgccgacgt tgtaggtttc gccaaggcgg ccggcgtcca ggacgcgcct gatcgcgcag
       61 caatggtcct tgacgtagag ccagtcgcgg atctgccggc cgtcgccgta gatcggcagc
      121 ggcttgccgg cgagcgcgtt gtggatgacc agcgggatca gtttttccgg gaaatggtag
      181 ggaccgtagt tgttcgagca gttggtggtc agcaccggca ggccgtaggt gtgatgatag
      241 gcgcgcacca gatggtcgga ggctgccttg ctcgccgagt acgggctgtt cggctcgtaa
      301 cggttggtct cggtaaaggc cggatcgtcg ctggcgagcg aaccatagac ttcgtcggtc
      361 gacacgtgca ggaagcggaa ggccgccttt tcctccgcac ccagtccgcc gcaatgggcg
      421 cgcaccgctt cgagcaggcg gaaagtgccg acgatgttgg tctggatgaa atcctccgga
      481 ccgtggatgc tgcggtcgac gtgcgattcg gcggcgaaat tgaccaccgc gcgcggccgg
      541 tgttcggcga gcagtttggc aaccaggtcg aagtcgccga tatcgccgtg gacgaagaga
      601 tggcgcggat cgccccgtag tgccgcaagg ttctcgagat tgccggcgta ggtcagcgcg
      661 tcgaggttga gcaccggctc ggcggattga gcgagccagt cgaggacaaa gttgctgccg
      721 atgaagccgg cgccgccggt gacgaggatc atgccgaaaa tccgccgcaa aaatggggca
      781 ttttacgcca agcgcacttc attcctgccg gcggcgcaaa aaactggcga agcgcggcaa
      841 acccttggcg gtcagctccc ggtagcgata agtgacgacg ctgccgatcg gcggcgggct
      901 cttgcgctgc gcatcggtaa agcccgtccc cagacggaat tcgcgaccgt ccgccaggcg
      961 cacgcgcaag gcgccgagtt gtccggcgta cttgcccttg cccggcagat gggcgacgac
     1021 gacggcctcg gcgtcgagcc agggtttgag cttgagcagg gtgtcggaac ggccggtttc
     1081 gtagggcgcg tcggcgcgat gcagcatcag gccttcgcca cccgccttga tggtttgcgc
     1141 cagccgctgg cgcagcgctt cccggctggc cagcgttgcc tgcggcacct cggccagcca
     1201 gggcaaggcc gcggcagcga cgattgcgcg gatgcgcccg gcccgctcgg cgaaagtccc
     1261 cggcgcgccg ggcagttcga agatcatgta gcggacttcc cgccactcgg catcgactgg
     1321 ctgctcgcgg cggacgatgc cggagaggcg ctcgaactgg ccacggccca gccacaactc
     1381 gccgtccatc ggcgttttcg gcagagcatc ggtgaaccag gccggcgcat tgacccggtt
     1441 gccgctgcga aaacgcagcg tctcgccgtc ccagacggcg cgcacgccgt cgagcttctc
     1501 gctgaccagg taggcggaaa cgtcgatgtc gtcgcgatag acgttggcca gcaggagttc
     1561 cggcggcgcg gcggcgagcg gcagggcaag gagcagcgcg agcccgcaga gcagcgcgcg
     1621 cagccgccgc atgctcagct tcccgcccac tcccggcgcg cctgacgggc gatgccgaag
     1681 acgtcctcga gacgctcgcc gtcggcgcgg gccagcgcgt cgcgcagctc gtcgagctgg
     1741 gcgcggtagc tgtcgagttc gccgagcagc gcctcgcggt tggcgaggca gatgtcgcgc
     1801 cacatttccg ggtggctggc ggcgatccgc gtgaagtcgc ggaagccgga ggcggcgaag
     1861 gtgaagaaca ggtcggcgtc gtcgcgcacc gccaggtcat ggaccagggc gaaggacagc
     1921 aggtgcggca ggtggctgac ggcggcgaag acgcggtcgt gcatttccgg cgtcagttcg
     1981 tagatatcgg cgccgcacag cgcccaggcg cgcttgacgc ggtcgaggcg ctcgtcgttg
     2041 ttctcgggca gcggggtgac gacgaccttc ctgccctgat agaggtcttg ccttgcggcc
     2101 gagggcccgc tgttctcggc gccggcaatc ggatgcgccg gcacgaactt gccgatgtgg
     2161 ccgcggaagg cggcacgcgc cgccgcgacg acgtcgccct tggtcgaacc gccgtcggtg
     2221 acaatggtgt tcggccc
//
LOCUS       DAOSDX010000486         2236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16842_length_2236_cov_2.362219, whole genome shotgun sequence.
ACCESSION   DAOSDX010000486 DAOSDX010000000
VERSION     DAOSDX010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16842_length_2236_cov_2.362219"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLN02_10525"
     CDS             <1..304
                     /locus_tag="PLN02_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl-[acyl-carrier protein]
                     O-methyltransferase BioC"
                     /protein_id="HOI52801.1"
                     /translation="ESGYADPVMDMEVLTLTYDDIDSLFAELRGAGSACAMHARRHGL
                     TGKGRLQAVREAYERMRSAGRLPASFEIIYGHAWKAAPKKTADGRAIIRFERGARP"
     gene            complement(309..971)
                     /locus_tag="PLN02_10530"
     CDS             complement(309..971)
                     /locus_tag="PLN02_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HOI52802.1"
                     /translation="MSTSSARIPVNAFKALLFVASLLPAAWIGWQLRHNGLGPEPSET
                     LQAFTGTWTLNFLLLTLCISPARALTGQHWLLRLRRMLGLFAFAYALLHLFSFIGYDH
                     GFDLTAIARDVLKRPFIAVGLAALLLMVPLAATSNATAIRRLGGRKWQELHRNIYLIA
                     ILGCVHLLWQSKPEELPAALSYAVFLGLLLWWRVQERRRKATPMPDQRVQAVKFYRKR
                     PD"
     gene            complement(990..1838)
                     /locus_tag="PLN02_10535"
     CDS             complement(990..1838)
                     /locus_tag="PLN02_10535"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOI52803.1"
                     /translation="MSAHDSLHFRILEDIAHELSGDINFPTCMDAALLVRNTLKDPFA
                     NIDRVVRVVSIEPLISSKLLRLANSIAYNPAGKTISDLGTAIGRIGFDSVRTISLAVA
                     MDQLLKSRNLAAYEPIAHRAWAHSLDVAAIARVLARRIGRINPEDAMMAGLVHDIGVF
                     YLLYRAAEYPEYRSNPDAMMELLCGWHEGIGESLLHALGVPERIIEAISDHDRLYNVE
                     TPCNVRDVLYFANLLAGGDREWMGEYSDADAQELRDADRARYQDLVLEAEDDIHELRS
                     ALGIRQ"
     gene            complement(1842..>2236)
                     /locus_tag="PLN02_10540"
     CDS             complement(1842..>2236)
                     /locus_tag="PLN02_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI52804.1"
                     /translation="VLAAVAPDDSVFHALCVMAEHNVGALLVLEDGRLVGIFSERDYA
                     RKQIRNNLSPKETPVREMMSSRVAYVSLDSTLEECMTLMTEKRFRHLPVLDDQQQVLG
                     ILSIGDMVKEIISSQQFLIEELERYIAS"
BASE COUNT          425 a          645 c          755 g          411 t
ORIGIN      
        1 cgaaagcggt tacgccgatc cggtgatgga catggaagtg ttgaccctga cctacgatga
       61 catcgacagc ctgttcgcgg agctgcgcgg cgccggttcg gcctgcgcga tgcacgccag
      121 gcgccacggc ctgaccggca aggggcgcct gcaggcggtg cgcgaggcct atgaacggat
      181 gcgcagcgct ggccgcttgc cggccagttt cgagatcatt tacggccacg cctggaaggc
      241 ggcgccaaag aaaaccgccg acggtcgcgc gatcatccgc ttcgaacgcg gcgctcgtcc
      301 ctgagtcgct aatccgggcg cttgcggtag aacttcaccg cctgtacccg ctggtcgggc
      361 atcggcgttg ccttgcgccg ccgctcctgg acccgccacc acagcagcag gccgaggaaa
      421 accgcgtagc tcagcgccgc cgggagctct tccggcttgc tctgccatag caggtgcacg
      481 cagcccagaa tggcgatcag gtagatgttg cggtgcagtt cctgccactt gcgcccgccc
      541 agccggcgga tggctgttgc gttcgaggtg gcggctagtg gaaccatcag cagcaaggcg
      601 gcgaggccga cagcgatgaa ggggcgcttg agcacgtcgc gggcgatcgc ggtgaggtcg
      661 aagccatggt cgtagccgat gaagctgaag aggtgtagaa gagcgtaggc aaaggcgaac
      721 aagccaagca tccggcgcag gcgcagcagc caatgctggc cggtgagggc gcgtgccggc
      781 gagatgcaca gcgtgagtaa cagaaaattc agcgtccagg taccggtgaa ggcttgcaag
      841 gtttcgctgg gctccgggcc aagcccgtta tggcgtagtt gccagccgat ccaggcggct
      901 ggcagcaggc tggcaacgaa gaggagtgcc ttgaaagcgt tgacgggaat acgcgcgctg
      961 ctagtgctca tcttgggtcc tgcggaggat tactgacgga tgccgagtgc cgaccgcaac
     1021 tcatggatat cgtcctcggc ttcgagaacg aggtcctggt aacgagcccg gtcggcgtcg
     1081 cgcaactcct gggcgtccgc gtcgctgtat tcccccatcc attcccggtc gcctccggcc
     1141 agcaggttgg caaaatagag gacgtcgcgc acgttgcaag gggtttcgac gttgtacagg
     1201 cggtcgtggt cgctgatcgc ttcgatgatt cgctcgggga ctccaagcgc atggaggagg
     1261 ctctcgccaa tgccttcgtg ccagccgcag agcaactcca tcatggcgtc cgggttgctg
     1321 cggtattccg gatactcggc agcccgatac agcaggtaga agacaccgat gtcgtgtacc
     1381 aggccggcca tcatggcgtc ttctggattg atgcggccga tccgccttgc cagtacccgg
     1441 gcgattgccg cgacatcgag cgaatgggcc caggcgcgat gcgcgatggg ctcgtaagcg
     1501 gcgaggttgc gtgacttgag caattgatcc atggctactg ccagcgagat cgtgcgcacc
     1561 gagtcgaagc cgatgcgtcc gatggcagtg cccaggtcgc tgatcgtctt gccggcgggg
     1621 ttgtaggcaa tcgagttggc gagacgaagc aacttgctgg agatcagggg ctcgatgctg
     1681 accacccgga caacccggtc gatgttggcg aaagggtcct tcaaggtatt gcgcacgagc
     1741 agtgcggcat ccatgcaggt cgggaaattg atgtcgccgg aaagctcgtg ggcgatgtcc
     1801 tcgagaatgc ggaaatgcag gctgtcgtgc gcgctcatga ctcaactggc gatgtaacgc
     1861 tcgagttcct cgatcaggaa ttgctgggag ctgatgattt ccttgaccat gtcgccgatc
     1921 gagagaatgc cgagcacctg ctgttgatcg tcgagcaccg gcagatgccg gaaacgtttt
     1981 tcggtcatca gcgtcatgca ttcttccagg gtgctgtcga gactgacgta ggcaactcgg
     2041 gagctcatca tttcgcgaac cggcgtttcc ttgggcgaga ggttgttcct gatctgcttc
     2101 ctggcgtagt cgcgctcgga aaagatgccg accagacgcc cgtcctcgag caccagcaag
     2161 gcgccgacat tgtgttcggc catgacgcaa agggcatgga aaacgctgtc gtcgggggcg
     2221 acagcggcaa ggacct
//
LOCUS       DAOSDX010000487         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16901_length_2230_cov_3.190345, whole genome shotgun sequence.
ACCESSION   DAOSDX010000487 DAOSDX010000000
VERSION     DAOSDX010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16901_length_2230_cov_3.190345"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="fliR"
                     /locus_tag="PLN02_10545"
     CDS             <1..804
                     /gene="fliR"
                     /locus_tag="PLN02_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOI52805.1"
                     /translation="AADRLIMLSLSTAQIDAWIVFYAYPLARTLGLIATVPLWSTAGI
                     PRRTRLILGLAIAVAIAPVLPAMPAVAPGSLDGLWILAQQMLIGIAMGFAARVVFAAV
                     DMAGTYMGFQMGLGFATFYDPLEGAQTPVLSEFIGLLSLLLFMALDGHLLFVSTLARS
                     FELIPVAPEALSPNSWRNLAELGGKIFSAGLLLCLPVLIALMITNIALGVLSKAAPQL
                     NLLALGFPLTLIGGFVVIGLSLRYLAVPFQELYDFAIRAMLEFAQPALQ"
     gene            complement(798..1241)
                     /locus_tag="PLN02_10550"
     CDS             complement(798..1241)
                     /locus_tag="PLN02_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52806.1"
                     /translation="MKPSILLVAACCVALGGCSTAGTGTFSLAANTNVKLGMALAAAA
                     AVYLIYDPLAPNWEIEEAKLADDSYRLSMKMKRFHTGGSGEALQVLKRRAQQLQEKNG
                     FAAYQIASYSEGIESQTPIAQRVAEGTIRLVKGETADSFALNGAY"
     gene            complement(1238..2158)
                     /gene="flgL"
                     /locus_tag="PLN02_10555"
     CDS             complement(1238..2158)
                     /gene="flgL"
                     /locus_tag="PLN02_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOI52807.1"
                     /translation="MRISTSMLFGTGTQGLQDLQSDLFKLQNQMSTGRRILTPSDDPI
                     GAWEAQQVSQSQSINNQYISNQDTAVSRLSYMEANLTNLEDGLARIYELAATGKGKGD
                     AQRAALVPELEELLQNVLTLANAKDGTGNYIYSGTMSSTKPFTASGSGIAYAGDQGQQ
                     QLRVSSSNVMEMGHNGFDVFMQVKDAQGNQTGSSIFETVQNLIDIFDPTSGVPFDETT
                     RAQLQDQLSVSIDHVANRIASVGTRLNTLDDLKVASKDFDLQYATRLQDLQEVDYTAA
                     ISQFSQLQFQLQAAQLTFKQSSQMSLFSIL"
BASE COUNT          351 a          702 c          701 g          476 t
ORIGIN      
        1 gcagctgatc ggctgatcat gctgagcctg agcacggcgc agatcgatgc ctggatcgtc
       61 ttctacgcct acccgctggc ccgcacgctc ggcctgatcg ccaccgtccc cctgtggagc
      121 accgccggca ttccgcgccg gacgcgcctg atcctcggcc tggcgatcgc cgtcgccatc
      181 gccccggtgc taccggcgat gccggcggtc gcccccggtt cgctcgacgg cctgtggata
      241 ctggcgcagc agatgctgat cggcatcgcc atgggctttg ccgcccgcgt cgtctttgcc
      301 gccgtagaca tggccggcac ctacatgggc ttccagatgg gcctcggctt cgccaccttc
      361 tacgatccgc tggaaggcgc ccagacgccg gtgctttcgg agttcatcgg actgctgtcg
      421 ctgctcctgt tcatggcgct ggacggccac ctgctgttcg tttccaccct ggcccgcagt
      481 ttcgagctga tcccggtggc gccggaagcc ttgtcaccca atagctggcg caacctggcc
      541 gaactcggcg gcaagatctt ttctgccggc ctgctgctct gcctgccggt cctgatcgcg
      601 ctgatgatca ccaacatcgc cctcggcgtg ctgagcaagg ctgcgccgca actgaacctg
      661 ctggccctcg gcttcccgtt gacgctgatc ggcggtttcg tggtgattgg cctgagcctg
      721 cgctatcttg ccgttccatt tcaggaactc tacgacttcg cgatccgggc gatgctggaa
      781 ttcgcccagc cggcgcttca gtaggcgccg ttcaacgcaa aggagtcggc ggtttcgccc
      841 ttgaccaggc ggatcgttcc ctcggcgacg cgctgggcga tcggcgtctg cgattcgatg
      901 ccttcgctgt aactggcaat ctggtaggcg gcaaagccgt tcttctcctg caactgctgc
      961 gcccgtcgct tgaggacctg caaggcctcg cccgagccgc cggtgtggaa gcgcttcatc
     1021 ttcatcgata gccggtagct gtcgtcggca agcttggctt cctcgatttc ccagttcggc
     1081 gccagcggat cgtagatcag gtaaaccgcg gcggcggcgg cgagcgccat gccgagcttg
     1141 acgttggtgt tggcggccag cgagaaagtg ccggtgcccg ccgtgctgca gccgccgagg
     1201 gcgacgcaac aggccgcgac gaggaggatc gaaggtttca caggatactg aacagactca
     1261 tctggctgga ttgcttgaag gtcaactgcg ccgcctgcaa ctggaactgc agttgcgaga
     1321 actgggagat ggccgcggtg taatcaactt cctgcaggtc ctgaagacgg gtggcgtact
     1381 gcaggtcgaa atccttgctc gccaccttca ggtcgtctag cgtgttgagt cgggtgccga
     1441 ccgaagcgat gcggttcgcc acatggtcga tggaaacgct caattgatcc tgcaactgcg
     1501 cccgcgtcgt ttcgtcgaaa ggaacgccgc tggtcggatc gaagatgtcg atcaggttct
     1561 ggaccgtctc gaagatcgaa ctgccggtct ggttgccctg ggcatccttg acctgcatga
     1621 agacatcgaa accgttgtgt cccatctcca tgacgttcga cgacgacacc cgaagctgct
     1681 gctgcccctg gtcgccggca taggcgatac cgctgccgga ggctgtgaac ggcttggtcg
     1741 acgacatggt gccgctgtag atgtaattgc cggtcccgtc cttggcgttg gccagggtca
     1801 ggacgttctg taacagctcc tcgagttcgg gaaccagtgc cgcccgttgc gcgtccccct
     1861 tgcccttgcc ggtggcggcg agttcgtaga tccgggccag gccatcctcg agattggtga
     1921 ggttggcttc catgtacgac agtcgcgaga ccgccgtgtc ctggttgctg atgtactggt
     1981 tgttgatcga ctgcgactgg ctgacctgct gggcttccca ggcgccgatc gggtcgtcgg
     2041 acggggtcag gatacggcgc ccggtcgaca tctgattctg cagcttgaac aaatcggact
     2101 gcaaatcctg cagcccctgc gttccggtgc cgaagagcat cgaggtactt atgcgcattt
     2161 tgctctccct taggatttga gcgacagcaa ggtgtcgaac agggtggtgc cgaccgacag
     2221 aatcttggct
//
LOCUS       DAOSDX010000488         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16922_length_2228_cov_3.112287, whole genome shotgun sequence.
ACCESSION   DAOSDX010000488 DAOSDX010000000
VERSION     DAOSDX010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16922_length_2228_cov_3.112287"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="PLN02_10560"
     CDS             <1..1797
                     /locus_tag="PLN02_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI52808.1"
                     /translation="KSSLLAALAAGSGKGRLDDGEVWVQPGIRVGYVPQEPPFDPAHS
                     VFEAVISGMGETSALLAEYHAVSHRLGEGGDHDALLTRMAQLQQELEARGAWAYEAQA
                     EKVIARFGLDPEAQVGSLSGGQKKRLALAQALAVAPEVLLLDEPTNHLDIAAIEWLET
                     LLIESGVTLLFITHDRSFLDRVSTRIVELDRGQLASFPGNFTQYQQRKEAMLHEEALA
                     NARADKLLKEEEVWIRKGVEARRTRAVFRVQRLDRLRAERQARRERMGRVNLQLESGD
                     KSGKLVAELDHVSKSWGARRIVSDFSTRIQRGDKIGLIGPNGAGKTTLLRLILGELAP
                     DAGTVRQGTKLEIAYFDQFRTQLNPDSTLADVISPGSDWVEIGGARKHVIGYLEDFLF
                     APERARSPVSSLSGGERNRLLLARLFAKPANVLVLDEPTNDLDIDTLELLEQLLQDYD
                     GTLFLVSHDRTFLDNVVTQTIAAEGDGVWKEYAGGYSDWANYQASLAKVAADKPKAET
                     KAAKPVEAAKTKVDKLSWKEQRELEALPEKIAALEAEQAKLTALLEDPGLYQRDAQGA
                     QNASARLATIDDELMALLERWEALEARAGNPS"
     gene            1817..>2228
                     /locus_tag="PLN02_10565"
     CDS             1817..>2228
                     /locus_tag="PLN02_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI52809.1"
                     /translation="MNLPRTSWRTPLLILVIGGLILTLSMGVRHTAGLFLQPMTMAHG
                     WSRETFSFAIAIQNLVWGLASPFAGAFADRHGAGRTVFVAALLYALGLVLMAQASSPL
                     AFDLSAGVLIGLGLSGTTFAVIMGVIGRHTTPERR"
BASE COUNT          389 a          775 c          738 g          326 t
ORIGIN      
        1 aagtcgtcgc tgctggcggc gctcgccgcc ggctccggca agggccggct cgatgacggc
       61 gaggtctggg tacagcccgg catccgcgtc ggctacgtgc cgcaggagcc gcctttcgat
      121 ccggcgcatt cggtgttcga ggcggtgatt tccggcatgg gtgagacttc ggcgctgctc
      181 gccgagtacc acgcggtgtc gcaccgcctg ggcgaaggcg gcgatcacga cgcgttgctg
      241 acccgcatgg cgcaactgca acaggaactg gaagcgcgcg gcgcctgggc ctacgaggcg
      301 caggcggaaa aggtcatcgc ccgcttcggg ctcgatccgg aagcgcaggt cggcagcctc
      361 tccggcggcc agaagaagcg cctggcgctg gcccaggcgc tagccgtggc accggaagtg
      421 ctgctgctcg acgagccgac caaccacttg gacatcgccg ccatcgaatg gctggaaacg
      481 ctgttgatcg aatccggcgt gacgctcctc ttcatcaccc acgaccgttc cttcctcgac
      541 cgcgtcagca cccgcatcgt cgagctcgat cgcggccagc tggcgagttt tcccggcaat
      601 ttcacgcagt accagcagcg caaggaagcg atgctgcacg aggaggcgct ggccaacgcg
      661 cgcgccgaca agctgctcaa ggaagaggaa gtgtggatcc gcaagggcgt cgaggcgcgg
      721 cgcacgcgcg cggtgttccg cgtccagcgc ctcgaccgct tgcgcgccga gcgccaggcg
      781 cggcgcgagc gcatgggccg cgtcaacctg cagctggaaa gcggcgacaa gagcggcaag
      841 ctggtcgccg aactggatca cgtcagcaag tcctgggggg cgcgccgcat cgtcagcgat
      901 ttctcgacgc gcatccagcg cggcgacaag atcggcctga tcggccccaa cggcgccggc
      961 aagacgacgc tgctgcgcct catcctcggc gaactggcgc cggacgcggg aacggtacgg
     1021 cagggcacga agctggaaat cgcctacttc gaccagttcc gcacccagct caatccggat
     1081 tcgacgctgg ccgacgtcat ctcgccgggc tccgactggg tcgagatcgg cggcgcccgc
     1141 aaacacgtca tcggctacct ggaagatttc ctgttcgcgc ccgagcgcgc ccgctcgccg
     1201 gtcagctcgc tctccggcgg cgaacgcaac cgcctgctgc tcgcccgcct gttcgccaag
     1261 ccggccaacg tgctggtgct tgacgagccg accaacgacc tcgacatcga caccctcgaa
     1321 ctgctcgaac aactcctcca ggactacgac ggcacgctct tcctggtcag ccacgatcgt
     1381 accttcctcg acaacgtcgt cacccagacc atcgccgccg aaggcgatgg cgtctggaag
     1441 gagtacgccg gcggctacag cgactgggcg aactaccagg cgagcttggc gaaggtcgcg
     1501 gctgacaagc cgaaagccga gaccaaggcg gcgaaaccgg tcgaagcggc gaagacgaag
     1561 gtcgacaagc tgagctggaa ggagcagcgc gaactggaag cactgccgga gaagatcgct
     1621 gcgctggaag ccgaacaggc gaagctgacc gcattgctcg aagaccccgg cctctaccag
     1681 cgcgacgcgc agggggcgca gaacgcctcg gcgcgtctgg caacgatcga cgacgaactg
     1741 atggccctgc tcgaacgctg ggaagcgctc gaggcgcgcg ccgggaaccc gagctgaaaa
     1801 cggcaatcga aacgccatga acctgccccg aacttcctgg cgcacgccgc tgctgatcct
     1861 ggtcatcggc ggcctgatcc tcaccctgtc gatgggcgtg cgccacaccg ccggcctctt
     1921 cctgcagccg atgacgatgg cgcacggctg gagccgcgag accttctcct tcgccatcgc
     1981 catccagaac ctggtctggg ggctggcctc gccgttcgcc ggggctttcg ccgaccgcca
     2041 cggcgccggc cgcaccgtgt ttgttgccgc gctcctctac gcgcttggcc tggtgctgat
     2101 ggcgcaggcg agttcgccgc tcgccttcga cctgtcggcc ggcgtgctga tcggtctcgg
     2161 cctgtcgggg acgaccttcg ccgtgatcat gggcgtcatc ggccgccaca cgacgccgga
     2221 gcggcgca
//
LOCUS       DAOSDX010000489         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16960_length_2224_cov_2.375288, whole genome shotgun sequence.
ACCESSION   DAOSDX010000489 DAOSDX010000000
VERSION     DAOSDX010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16960_length_2224_cov_2.375288"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2224
                     /locus_tag="PLN02_10570"
     CDS             <1..>2224
                     /locus_tag="PLN02_10570"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HOI52810.1"
                     /translation="FVDLPLYPWQRERFWQESSPEGYGLIVRDRVHPLLGWALKEAPA
                     AWENLLDPRVDRWLADHRVGGAVVLPGAAYVEMALAASQQFYGGDSIEVEQLDIISPV
                     VFDEAHGRCIRFDLNPRDGSFQIRSRQRLSEDEWSLHAAGRLLGTMSVALTTRPLLAI
                     DPVAEIDHDTHYRLASELGLDYGGDFRGLARACHDDTRLSGEFVAATEADAWLLTPAR
                     LDVCFQSLLDFQRSEIESGNGVPSLPVKIGRLQLRLGAGPVVAFRTYLRKRSLRSVLV
                     DFELFDAAGQCVARLAGCRFRSAALRREGRTAPAAWEIVPTLAAHPSATRQAAFPGGA
                     DWAAILDRALLAQEPRLQRQSAIGEALPLLDALVTAYALQAMAGLLREAPEDAGRFLA
                     GEGFASDAQRALFAWTRTLLESQELLQIDDGVPELDADALPAPAEIWRTLLAEYPDSL
                     AELILLAVAGSRLADVLAGRGDAGALAAALARSPQWETLHDDSLSYLASRVACETLVV
                     EAMRRRDPARRLRVLEIAAGATTIPRQIAGAIDATGIDYWIAQADPEIRDRLSRDYLE
                     RDWVQVAALDAEAARLDAAALQGGDFDLILLRHALSASANPRALLDMLQGVLAEGGLL
                     AISERAADPAAGLLAAGRLLPGAGAWQTLLEGSGFATCRLWYEPAAADLAAGSYLLLA
                     EGGARAGEPAPESPPAVAEHWLLLAADPAATGVVALQAALAASGKNVEIASELPATAS
                     P"
BASE COUNT          305 a          810 c          738 g          371 t
ORIGIN      
        1 ttttgtcgat ttgcccctct atccctggca acgcgaacgt ttctggcaag agagctcgcc
       61 tgagggttac ggcctgatcg tacgtgaccg cgttcatccg ctgctcggct gggcgctcaa
      121 ggaagcgccg gccgcctggg agaacctgct cgatccgcgc gtcgaccgct ggctggccga
      181 ccaccgcgtc ggcggtgccg tcgtcctgcc cggcgccgcc tacgtcgaga tggcgctggc
      241 cgcgtcgcag cagttctacg gcggcgacag catcgaggtc gaacagctcg acatcatttc
      301 gccggtggtc ttcgacgaag cccatggacg ctgcatccgc ttcgacctca acccgcgcga
      361 cggcagtttc cagatccgca gccggcaacg gctgagcgag gacgaatggt cgctgcacgc
      421 cgccggccgc ctgctcggta cgatgagcgt ggcgctgacg acgcgtccgc tgctcgccat
      481 cgacccggtt gctgaaatcg atcacgacac ccactaccgg ctggccagtg aactcggcct
      541 cgactatggc ggcgatttcc gtggcctggc tcgtgcctgc cacgacgata cccggttgag
      601 cggcgagttc gtcgccgcca cggaagccga cgcctggctg ctgacccccg ctcggcttga
      661 tgtctgcttc cagtcgctgc tcgattttca acgttcggaa atcgagtccg gcaacggtgt
      721 tccctcgcta ccggtcaaaa ttggtcgcct gcaactgcgc ctcggcgctg gaccggtcgt
      781 cgcattccgt acttatttgc gcaagcgcag cctgcgttcg gtgcttgtcg atttcgaact
      841 gttcgacgcc gccggccagt gcgtcgcccg ccttgccggc tgccgcttcc gttcggcggc
      901 actgcgccgg gaagggcgga cggcaccggc ggcctgggaa atcgtgccga ctctggcggc
      961 gcatccgtcg gcaacccgcc aggcagcttt ccctggcggc gccgactggg cggccatcct
     1021 cgaccgggcg ctgcttgccc aggagccgcg cctgcagcgc cagtcggcta tcggcgaagc
     1081 cctgccgctg ctcgacgcgc tggtcaccgc ctacgcgctg caggcgatgg ccgggctact
     1141 gcgcgaggcc ccggaggatg ccgggcgctt ccttgccggc gagggttttg ccagcgatgc
     1201 gcagcgggcc ctgttcgcct ggacacggac tctgctggaa agtcaggaac tgctgcagat
     1261 cgatgacggc gttcccgaac tcgatgccga tgccttgccg gcaccagccg aaatctggcg
     1321 cactctgctt gccgagtacc cggacagcct ggccgagctg atcctgcttg ccgtcgccgg
     1381 cagccggctg gccgatgtcc tggccgggcg tggcgatgcc ggtgcgcttg ctgccgcgct
     1441 cgcgcgcagc ccgcaatggg aaaccctgca cgacgattcg ctgagctacc tggccagccg
     1501 cgtcgcctgc gaaaccctgg tcgtcgaggc gatgcgccga cgcgacccgg cgcgccgcct
     1561 gcgcgtcctg gaaatcgccg ccggcgcgac gacgattccg cgccagatcg ccggcgccat
     1621 cgacgccacc ggcatcgact actggatcgc ccaggccgac ccggaaattc gcgaccgcct
     1681 gagccgcgat tacctggagc gcgactgggt gcaggtcgct gcgctcgatg ccgaggccgc
     1741 gcgtctcgac gccgccgcct tgcagggcgg tgatttcgac ctcatcctgc tgcgtcatgc
     1801 cttgtcggcc agcgccaacc cgcgcgcgct gctggacatg ctgcagggcg tgctggccga
     1861 gggcggcctg ctcgccatca gcgaacgggc ggccgatccg gccgccggcc tgctcgctgc
     1921 cggccgcctg ctgcccgggg ccggcgcctg gcagacactg ctcgaaggca gcggttttgc
     1981 cacctgccgg ctctggtacg aaccggccgc cgccgacctc gctgccggca gctacctgtt
     2041 gctggccgaa ggcggcgcgc gcgcaggcga gccagcgccg gaatcgccgc cagccgtcgc
     2101 cgaacactgg ctgctgctcg ctgccgatcc ggctgccacc ggcgttgtcg cgttgcaggc
     2161 cgcactggcc gccagcggca agaacgtgga aattgccagc gaactgccgg ccaccgccag
     2221 ccca
//
LOCUS       DAOSDX010000490         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_16998_length_2220_cov_3.177829, whole genome shotgun sequence.
ACCESSION   DAOSDX010000490 DAOSDX010000000
VERSION     DAOSDX010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16998_length_2220_cov_3.177829"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLN02_10575"
     CDS             complement(<1..362)
                     /locus_tag="PLN02_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HOI52811.1"
                     /translation="MSINDPRWGNRGGDDGQDGGGPRRPNDGPPDLEELWRDFNRKLS
                     GMMGKKRGGGNGGGDGPRMPPVDFGPKMLGGGIGVLLGLVVLVWLASGFYIVYESQRG
                     VVLQFGKRYETTEPGLRWR"
     gene            complement(400..1554)
                     /gene="hflX"
                     /locus_tag="PLN02_10580"
     CDS             complement(400..1554)
                     /gene="hflX"
                     /locus_tag="PLN02_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOI52812.1"
                     /translation="MLERPAAGERAVVVQLDFGQLDVEDQLEEVRLLTESAGGAVVGE
                     VRGRRQSPDPKTFAGSGKVQEIAAMIAGCSAQLVIFNHALSPVQQRNLERDLNCRVVD
                     RNSLILDIFALRAASAEGKLQVELAQLEHLSTRLVRGWTHLERQRGGIGMRGPGETQL
                     ETDRRLLGNRVKLLKERLARLARQRGVQRKARRRGDVLNVSLVGYTNAGKSTLFNTLT
                     HAGVYAADQLFATLDTTSRKLWVEGAGNIVISDTVGFIRDLPHSLVDAFHATLEAAVD
                     ADLLLHVVDSASHARDEQMAEVDRVLDEIGARDVPRLVVWNKIDLTEAGPGVERDEYG
                     NIARVRVSSRTGEGLELLRETLAEHARAKTASRRQAQDAEACPANPDFID"
     gene            complement(1612..1848)
                     /gene="hfq"
                     /locus_tag="PLN02_10585"
     CDS             complement(1612..1848)
                     /gene="hfq"
                     /locus_tag="PLN02_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOI52813.1"
                     /translation="MSNKGQLLQDPFLNALRREHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVNIHQEQAAE"
     gene            complement(1944..>2220)
                     /locus_tag="PLN02_10590"
     CDS             complement(1944..>2220)
                     /locus_tag="PLN02_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOI52814.1"
                     /translation="TPRLTRVLSDAIARQAPPKHGLFRPKPRYAHQGGSNPPIIVIHG
                     NAVDQIGDSYRRYLEHTFREAFKLQGTPLRIQFVAGKNPFAEKEPRR"
BASE COUNT          389 a          691 c          718 g          422 t
ORIGIN      
        1 cgccagcgca ggcccggctc ggtcgtctcg taacgcttac cgaactggag gacgacgccg
       61 cgctgcgatt cgtagacgat gtagaacccc gaggccagcc agaccaggac gaccagaccg
      121 agcaggacac cgatcccgcc acccagcatc ttcggcccga agtcgaccgg cggcatgcgc
      181 ggcccgtcgc caccaccgtt gccgccgccc cgtttcttgc ccatcatccc ggacagcttg
      241 cggttgaaat cgcgccagag ttcttcgaga tccgggggac catcgttcgg ccggcgcgga
      301 ccgccgccgt cctggccgtc gtcgccaccg cggttgcccc agcgcggatc gttgatcgac
      361 atgagtatac ctagcgtagg aatcatgatt tttggggatt cagtcaatga aatcagggtt
      421 tgccggacag gcttccgcat cttgcgcctg gcgccgcgac gcggtcttgg cccgggcgtg
      481 ttcggccagg gtttcgcgca atagttccag gccttcacca gtgcgcgaac tgacgcgaac
      541 ccgggcgata ttaccatatt cgtcccgctc gaccccgggc ccggcctcgg tcaggtcgat
      601 cttgttccag accaccaggc gcggcacatc gcgcgcgccg atctcgtcga gcacccggtc
      661 gacctcggcc atctgctcgt cacgggcgtg gctcgcgcta tcgacgacgt gcagcagcag
      721 atcggcatcg accgccgctt ccagcgtcgc gtggaaggca tcgaccagcg agtgcggcag
      781 atcgcggatg aaaccgacgg tatcggagat gacgatattg cctgcccctt cgacccacag
      841 cttgcgtgag gtcgtgtcga gggtggcgaa caactggtcg gcggcgtaaa caccggcatg
      901 ggtcagcgtg ttgaacaagg tcgacttgcc ggcgttggta tagccgacca gtgaaacgtt
      961 gagcacgtcg ccgcgccgcc gcgccttgcg ctgcacgcca cgctggcgcg ccaggcgagc
     1021 caggcgctcc ttgagcagct tgacgcggtt gccaagcaga cggcggtcgg tttccagctg
     1081 ggtttcgccg ggaccacgca tgccgatgcc gccacgttgc cgctcgaggt gggtccagcc
     1141 gcgcaccagg cgggtcgaca gatgttcgag ctgcgccagt tcgacctgca gcttgccctc
     1201 ggcgctcgcc gcgcgcagcg cgaagatgtc gaggatcagg ctgttgcgat cgaccacccg
     1261 gcagttcagg tcgcgctcga gattacgctg ctgcaccggc gacagcgcgt gattgaagat
     1321 caccagctgc gctgagcagc cggcgatcat ggcggcaatt tcctgcacct tgccgctgcc
     1381 ggcaaaggtc ttcgggtccg gcgactgacg tcgcccgcga acttcgccga ccaccgcacc
     1441 tcccgcggat tcggtcaaca ggcgaacttc ttcgagctgg tcttcgacat cgagctggcc
     1501 gaaatcgagt tgtacgacaa cggcccgctc accggcagcc ggccgttcaa gcatcggaaa
     1561 ttcctgcgga gaaaacagcc ccggacatcg gaggctgcca gatcagccgc cttattctgc
     1621 ggcctgctct tgatggatgt tgaccggacg cgccgggaca accgtggaga tggcgtgctt
     1681 gtacaccatc tgggtgacgg tgttcttgag gaggacgacg tactggtcaa aagactcaac
     1741 ctgcccctgc agcttgatgc cgttcaccag atagatggaa acgggaacat gctcgcgacg
     1801 cagtgcgttg agaaaggggt cttgtagaag ttgccctttg ttgctcatgg tgtggaactc
     1861 catgtgtgtt gttatgaggg tcctaatgta cccaaattag tcggtggatg cgaaaatttt
     1921 tgtgcgttat ttgtgacaat gattcagcgg cgtggctcct tctcggcaaa cgggttcttg
     1981 ccggcgacga actggatgcg caggggcgtt ccctgcagct tgaaggcttc gcggaaggtg
     2041 tgctcgaggt agcggcgata gctgtcgccg atctggtcga cggcattgcc gtggatgacg
     2101 atgatcggcg ggttcgagcc cccctggtgc gcatagcgcg gtttgggccg gaacaggccg
     2161 tgttttggcg gcgcctggcg ggcaatggca tcactcagga cgcgggtcag acgcggcgtt
//
LOCUS       DAOSDX010000491         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17013_length_2218_cov_3.631993, whole genome shotgun sequence.
ACCESSION   DAOSDX010000491 DAOSDX010000000
VERSION     DAOSDX010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17013_length_2218_cov_3.631993"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLN02_10595"
     CDS             <1..463
                     /locus_tag="PLN02_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOI52815.1"
                     /translation="TGVVACNALAVGLILRRLLARASTAYLLVPLFLLCGLVVRMLGA
                     AILIGPEEAMAWLTPGARQGLLIAAASLAVSLWLPPRFSLPLAVVALLAGTLLVNTAP
                     ANPYSEAALAAWRQGHFLNFNGLTRLVASSWPILTLPFLLLAMRNGTGRSM"
     gene            510..818
                     /locus_tag="PLN02_10600"
     CDS             510..818
                     /locus_tag="PLN02_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOI52816.1"
                     /translation="MSYFQHHVFICTNQRENGEACCNNVGGSAAFAYAKDRIGALKKN
                     GPGAVRINKAGCLGRCDQGPVMVVYPEETWYGFVDNEDVEEIIQEHLINGRVVERLKL
                     "
     gene            815..1453
                     /locus_tag="PLN02_10605"
     CDS             815..1453
                     /locus_tag="PLN02_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HOI52817.1"
                     /translation="MRAPEEKINVAGPAGNIEVIMERPDAPRGIALIAHPHPIGGGAN
                     TNKVAYTLAKTFVSLGYAAFRPNFRGVGGTQGVHDEGRGETDDLLAVLHDAKLRCGDI
                     PVALAGFSFGAYCQTRVARRLEESGHPAQRLVLVGTAAGYVEGTRQYDTEAVPHDSIV
                     IHGSNDETVPLANVFAWAQPLDLPVVVVPGADHFFHRRLHQIRDIVTRAWRH"
     gene            1441..>2218
                     /locus_tag="PLN02_10610"
     CDS             1441..>2218
                     /locus_tag="PLN02_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI52818.1"
                     /translation="MAALNVSGLRKRYGDNEVVAGLSFAVEPGTCFGLLGPNGAGKTT
                     TLRLCLGLTAPDGGTITLAGRPIPAEAQQARAKVGVVPQFDNLDPDFTCAENLRVFGR
                     YFGLKDAEVRARIPTLLDFASLANKADAKIATLSGGMKRRLTLARALVADPDIVFLDE
                     PTTGLDPQARHLIWERLKQLKSAGKTLILTTHFMDEAERLCDRLIVIDHGRKIAEGSP
                     RELIATHIEPQVIEVFDEAAGKLPAFVDAHRQHAERVEASG"
BASE COUNT          392 a          809 c          676 g          341 t
ORIGIN      
        1 aaccggggtc gtcgcctgca atgcgctggc cgtcggcctg atcctgcgcc gcttgctggc
       61 ccgggcatcg accgcctacc tgctggtacc gttgttcctg ctctgcggcc tggtcgtccg
      121 catgctcggc gcggcgatcc tgatcggccc ggaagaagcc atggcatggc tcaccccggg
      181 cgcccgccag ggcctgctga tcgccgccgc cagcctggcc gtgagcttgt ggctgccgcc
      241 caggttcagc ctgccgctgg ccgtcgtcgc cttgctggcg ggcacgctgc tggtcaacac
      301 ggcgccggcc aacccgtatt ccgaagccgc tctcgccgcc tggcgccagg gccacttcct
      361 gaatttcaac ggactgaccc ggttggtggc aagctcctgg cccatcctga cgcttccctt
      421 cctgttgctg gccatgcgca acggaacggg tcgttccatg tgaaaccggc gccgctcttt
      481 ataatccaac aacaaggagg tttcccccaa tgagctattt ccagcaccac gtattcatct
      541 gcaccaacca gcgcgaaaac ggcgaggcct gctgcaacaa cgttggcggc agcgcggctt
      601 tcgcctacgc caaggaccgc atcggcgcac tgaagaagaa cggccccggc gccgtgcgca
      661 tcaacaaggc cggctgtctc ggccgttgcg accagggacc ggtgatggtc gtctatcccg
      721 aagagacctg gtacggcttc gtcgataacg aggacgtcga ggaaatcatc caggaacacc
      781 tgatcaacgg acgcgtcgtc gaacgcctga agctatgaga gcccccgaag aaaagatcaa
      841 cgttgccggc ccggccggca acatcgaagt catcatggag cgcccggacg cgccgcgcgg
      901 catcgcgttg atcgcgcatc cacacccgat cggcggcggc gccaacacca acaaggtcgc
      961 ctacacgctg gccaagactt tcgtcagcct gggttacgcc gccttccgtc cgaacttccg
     1021 tggcgtcggc ggcacgcaag gcgtgcacga cgagggcagg ggagagaccg acgacctgct
     1081 cgccgtactg cacgacgcca agctgcgttg cggcgacatc ccggtcgccc tggccggctt
     1141 ctcgttcggt gcctactgcc agacgcgcgt cgccaggcgc ctggaggaaa gcggtcaccc
     1201 ggcgcagcgc ctggtcctgg tcggcaccgc tgccggctac gtcgaaggca cgcgccagta
     1261 cgacaccgaa gcggtaccgc acgacagcat cgtcatccac ggatcaaacg acgagacggt
     1321 gccgctggcc aacgtcttcg cctgggcgca gccgctcgac ctgccagtgg tcgtcgtccc
     1381 cggcgccgac catttcttcc accggcggct gcaccagatc cgcgacatcg tcacccgcgc
     1441 atggcggcac taaacgtttc cggactgcgc aaacgctacg gcgacaacga ggtcgtcgcc
     1501 gggctgtcgt tcgccgtcga acccggcacc tgcttcggcc tgctcggccc caacggtgcc
     1561 ggcaagacga cgacgctgcg cctctgcctc ggcctgaccg cgccggacgg cggcacgatc
     1621 accctggccg gccggccgat tccagccgaa gcgcagcagg caagggccaa agtcggcgtc
     1681 gtgccgcagt tcgacaacct cgacccggac ttcacctgcg ccgaaaacct gcgcgtcttc
     1741 ggccgctatt tcgggctcaa ggatgccgag gtacgcgccc gcatcccgac gctgctcgat
     1801 ttcgccagcc tggcgaacaa ggccgacgcg aaaatcgcca ccttgtccgg cggcatgaaa
     1861 cggcgcctga ccctggcccg ggcgctggtc gccgaccccg acatcgtctt cctcgacgag
     1921 ccgaccaccg gtctcgaccc gcaggcccgt cacctgatct gggaacgcct caagcaactg
     1981 aaatcggccg gcaagacgct gatcctgacc acccatttca tggacgaggc ggagcgcctg
     2041 tgcgaccgtc tgatcgtcat cgaccacggg cgcaagatcg ccgaaggcag cccgcgcgaa
     2101 ctgatcgcca cgcacatcga accacaggtg atcgaggtct tcgacgaggc cgccggcaaa
     2161 ctgccggcct tcgtagacgc ccaccgccag cacgccgaac gcgtcgaggc cagcggcg
//
LOCUS       DAOSDX010000492         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17043_length_2214_cov_3.455767, whole genome shotgun sequence.
ACCESSION   DAOSDX010000492 DAOSDX010000000
VERSION     DAOSDX010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17043_length_2214_cov_3.455767"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLN02_10615"
     CDS             <1..496
                     /locus_tag="PLN02_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOI52819.1"
                     /translation="NGQPITITDPTMTRFMMTLADAVDLVLYAFEHGNNGDIFVQKAP
                     AATVDVLARAVLELMGKPEHPVNVMGTRHGEKLYEALLSREEMACAEDMGDYFRVPPD
                     GRDLNYSKYVDQGEQRLTQTAHGEDYNSHNTTRLDVEGMKKLLLKLDFMQKIARGEYA
                     VAEE"
     gene            501..1628
                     /locus_tag="PLN02_10620"
     CDS             501..1628
                     /locus_tag="PLN02_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HOI52820.1"
                     /translation="MKVLITGAAGFVGRNLQLFLAERKDVEVVSFEREDSLDDLPSKL
                     AGVDFVFHLAGVNRPQDPQEFVTGNTDLTSVLCEAVGRAVREGGKAIPVIYTSSIQAD
                     RDNPYGQSKLGAEQVLRNAGQAFGFPVYLFRLPNVFGKWCRPNYNSAVATFCHNIARD
                     LPIQVNDPAAPVTLVYIDDVVSRFIQLMDGADPQFTPEGFVTVTPQYSTTVGELAGLI
                     RSFRESRNTLITERVGTGLVRALYSTYVSYLPKEAFAYPVTQHGDARGVFVEMLKTPD
                     CGQFSFFTAHPGITRGGHYHHTKTEKFLVIKGKARFKFRHMQSGECYELFTSGEKSEI
                     VETVPGWTHDITNVGDDEMVVMLWANEIFDREHPDTFAAPL"
     gene            1655..>2214
                     /locus_tag="PLN02_10625"
     CDS             1655..>2214
                     /locus_tag="PLN02_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOI52821.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSRVLARLDEHCEHVLVHTGQNYDYE
                     LNQVFFDDLGVRKPDHFLNSASGSSGAAHTIGNLIIAVDQVLATENPEAMLVLGDTNS
                     CLAVIPAKRRKIPIFHMEAGNRCFDLRVPEETNRRIVDHTADINLTYSTIARDYLLRE
                     GLAPDQVIKTGSPMFEVLNHYRPRIEA"
BASE COUNT          446 a          642 c          680 g          446 t
ORIGIN      
        1 caacggtcag ccgatcacga tcaccgatcc gacgatgacg cgcttcatga tgacgctggc
       61 cgatgcggtt gatctcgtcc tgtacgcttt cgaacacggc aacaacggcg acatcttcgt
      121 gcagaaggcg ccggcggcta cggtggatgt cctggctcgc gctgttctcg aactgatggg
      181 caagccggaa catccggtga atgtcatggg tacccgccac ggcgagaagc tttacgaagc
      241 gctgctgagc cgcgaggaaa tggcttgtgc ggaagacatg ggcgattatt tccgcgtgcc
      301 gccggacggt cgcgatctga attactcgaa gtacgtggat caaggcgaac aacgcctgac
      361 gcagacggcg cacggcgagg actacaactc gcacaacacg acccggctcg atgtcgaggg
      421 gatgaagaag ttgctgttga agctcgattt catgcagaag attgcccgcg gcgagtacgc
      481 ggttgccgag gagtaatccg atgaaggtgc tgatcactgg ggcagccggc tttgtcggcc
      541 ggaacctgca gttgttcctg gcggaacgca aggatgtcga ggtggtttcc ttcgaacgcg
      601 aggacagcct ggatgacctg ccgtccaaat tggccggtgt cgatttcgtg tttcacctgg
      661 ctggcgtcaa tcgcccgcag gatccgcagg agttcgtgac cggcaacacc gacctgacgt
      721 cggtattgtg cgaagcggtc ggccgtgcgg tgcgggaagg cggcaaggcg attccggtga
      781 tttacacttc gtcgatccag gcggatcgcg acaaccctta cgggcagagc aagctggggg
      841 ccgagcaggt cttgcgcaac gccggtcagg cgttcgggtt cccggtttat ctcttccgtt
      901 tgccgaatgt cttcggcaag tggtgccggc cgaattacaa ctcggcggtg gccactttct
      961 gccacaacat cgcccgcgat ctgccgatcc aggtcaacga tccggcggcg cctgtcactc
     1021 tggtttatat cgacgatgtc gtctcgcgtt tcatccagtt gatggacggt gccgatccgc
     1081 agttcacgcc ggagggcttt gtcacggtga cgccgcagta ctcgacgacg gttggcgagc
     1141 tggccggact gatccgctcc ttccgggaaa gtcgcaatac gctgattacc gagcgtgtcg
     1201 gcacgggctt ggtgcgcgcc ctgtattcca cctatgtcag ctatctgcct aaggaggcgt
     1261 ttgcctaccc ggtgacgcaa catggcgacg cgcggggtgt cttcgtcgag atgctcaaga
     1321 cgccggattg cggccagttt tctttcttca cggcacatcc ggggatcacc cgcggtgggc
     1381 attatcacca caccaagacc gagaagttcc tggtcatcaa gggcaaggcg cgcttcaagt
     1441 tccgtcacat gcagagcggc gagtgttacg aactttttac ttccggtgag aagtcggaga
     1501 tcgtcgaaac ggttcccggc tggacccatg acatcaccaa tgtcggcgat gacgagatgg
     1561 tcgtcatgct gtgggccaac gaaatcttcg accgcgagca tccggatact tttgctgcgc
     1621 cgctctgaac aaatcacttt tcgaacgcat aacgatgaaa aaactcaaag tcatgaccgt
     1681 ggtcggtacc cggccggaaa tcattcgcct gtcgcgtgtg ctggcgcgcc tcgacgagca
     1741 ttgcgagcac gtgctggtgc ataccgggca gaattacgat tacgaactga accaggtctt
     1801 ctttgacgat ctgggtgtgc gcaaaccgga tcatttcctg aacagcgcga gcggcagcag
     1861 tggcgcggcg cataccatcg gcaacctgat catcgccgtg gatcaggtgc tggcgaccga
     1921 gaatccggaa gcgatgctgg tgctgggcga taccaacagc tgcctggcgg tgatcccggc
     1981 caagcggcgc aagatcccga ttttccacat ggaagccggc aaccgctgtt tcgatctgcg
     2041 cgtccccgag gaaaccaacc gtcgtatcgt cgaccacacg gccgacatca atctgaccta
     2101 cagcaccatc gcccgcgact acctgctgcg tgaaggtctg gcgcccgacc aggtgatcaa
     2161 gaccggcagc ccgatgttcg aagtcctgaa tcactaccgg ccgcggatcg aggc
//
LOCUS       DAOSDX010000493         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17073_length_2211_cov_3.119666, whole genome shotgun sequence.
ACCESSION   DAOSDX010000493 DAOSDX010000000
VERSION     DAOSDX010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17073_length_2211_cov_3.119666"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..516)
                     /locus_tag="PLN02_10630"
     CDS             complement(58..516)
                     /locus_tag="PLN02_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HOI52822.1"
                     /translation="MNTTSRLFSSAVLAVFLALTSLTSAVAEPKLKSLRGSEIQGADT
                     ESADPFKSEKDRAPIERNYVQQPPLIPHKVETYTVTKNFNKCMDCHAWTRYKETGATK
                     VSLTHFKTRDGQELSNISPRRYFCLQCHVPQADAKPLVGNRFQAAEGIGH"
     gene            complement(513..1388)
                     /gene="napH"
                     /locus_tag="PLN02_10635"
     CDS             complement(513..1388)
                     /gene="napH"
                     /locus_tag="PLN02_10635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HOI52823.1"
                     /translation="MTPGREAIRSKGWWAAQRWLLLRRASQLLVLSLFLSGPWFGLWI
                     AKGNLASSLTFDLLPLSDPFVLLQSLAAGFMPALSGLLGAALVLVAYNLIGGRVFCAW
                     VCPVNAVTDSAAWLRRRLAIKTGRVPDAATRYWLLGAVFGLAALSGSLVWETVNPVHL
                     LQRSLIQGLAGALAGGMTMTLSVLLLVFLYDLLVASRGWCGHLCPMGAFYGLLGPKGL
                     LRVAASQRAACNDCMDCFAVCPEPQVIRPALKQDGQVSPVILDRDCTACGRCIDVCAK
                     NVFNFTHRFDQRSDS"
     gene            complement(1385..2203)
                     /gene="napG"
                     /locus_tag="PLN02_10640"
     CDS             complement(1385..2203)
                     /gene="napG"
                     /locus_tag="PLN02_10640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HOI52824.1"
                     /translation="MKPEPTHQQRKETGALASQARRKFLNRIAAAAGGAGALALGSLF
                     AMPPAAARPAQALRPPGALPEAEFAGACLRCGLCVQACPYGTLRLATPGDGLATGTPV
                     FTARNVPCEMCEEIPCIKACPSSALDHRLTEINNARMGLAVLIDHESCLNFLGLRCDV
                     CYRVCPVIDQAITLEKLHNPRSDRHALLLPTVHADHCTGCGKCEKACVLPTPAIKVLP
                     RPLAQGALPEHYRKGWEEKERHGGSLIGEQVQMPVRGLEDKAPGDTRLNSDWKP"
BASE COUNT          362 a          685 c          810 g          354 t
ORIGIN      
        1 atggccttga gacgggatat gagtgcttgc atgatgatct tcctccctgg tcggcgctca
       61 gtggccgatg ccttcggcgg cctggaaacg gttgccgacc agcggcttgg cgtcggcctg
      121 cgggacgtgg cactgcaggc agaagtagcg gcgcggcgag atgttggaga gttcctggcc
      181 gtcgcgcgtc ttgaagtggg tcagcgagac cttggtcgcg ccggtttcct tgtagcgggt
      241 ccaggcgtgg caatccatgc acttgttgaa gttcttggtc accgtgtagg tctcgacctt
      301 gtgcgggatc agcggcggct gctggacgta gttgcgttcg atcggcgccc ggtccttctc
      361 ggatttgaag gggtcggcgg actccgtgtc ggcgccctgg atttcgctgc cgcgcagcga
      421 tttcagcttc ggttcggcaa ctgccgaggt cagcgaggtg agggccagga aaaccgccag
      481 gacggcggag gaaaacaggc ggctcgtagt gttcatgaat cgctcctttg gtcgaaccga
      541 tgagtaaagt tgaagacgtt cttggcgcag acgtcgatgc aacgtccgca ggcagtgcag
      601 tcccggtcga gaatcaccgg ggaaacctgg ccgtcctgct tgagtgccgg acggatcacc
      661 tgcggctcgg ggcagacggc gaaacagtcc atgcagtcgt tgcaggcggc gcgctggctg
      721 gccgccacgc ggagcagccc cttgggcccg agcaggccgt agaaggcgcc catcggacag
      781 aggtggccgc accagccgcg gctggcgacc agcaggtcgt agagaaaaac cagtagaagc
      841 acgctcaggg tcatcgtcat gccgccggcg agggcgccgg ccagcccctg gatcaggctg
      901 cgctgcagca ggtgcaccgg gttgacggtt tcccagacca ggctgccgct cagcgcggcg
      961 agcccgaaga cggcgccgag cagccagtag cgggtggcgg cgtcgggcac acggccggtc
     1021 ttgatggcga gccggcgacg cagccaggcc gcgctgtcgg tgactgcgtt gaccgggcag
     1081 acccaggcgc agaagacgcg gccgccgatc aggttgtagg cgacgagaac cagcgccgcg
     1141 ccgagcagcc cgctgagtgc cggcatgaag ccggcggcca gcgactgcag caggacgaag
     1201 gggtcgctca gcggcagcag gtcgaaggtc agcgacgagg ccaggttgcc cttggcgatc
     1261 cacaggccga accacggccc gctgaggaac agcgagagca ccagcaactg gctggcgcgg
     1321 cgcagcagca accagcgctg ggcggcccac cagcccttgc tgcggatggc ttcgcgaccg
     1381 ggggtcatgg tttccaatcc gagttgaggc gagtgtcgcc gggagccttg tcttccaggc
     1441 cgcgtaccgg catctgcacc tgttcgccga tcagcgatcc gccgtggcgc tccttctctt
     1501 cccagccctt gcggtaatgc tcgggcagcg cgccctgggc gagcggcctg ggcagcacct
     1561 tgatcgctgg cgtcggcaag acacaggctt tttcgcactt gccgcagccg gtgcaatgat
     1621 cggcgtggac agtgggcagg agcagcgcgt ggcggtcgga gcgcgggttg tgcagctttt
     1681 ccagggtgat cgcctgatcg atcaccgggc agacccggta acagacgtcg caacgcaggc
     1741 ccaggaagtt gaggcaggat tcatggtcga tcagcaccgc cagacccatc cgggcgttgt
     1801 tgatctcggt cagccgatgg tcgagcgccg acgacgggca ggccttgatg cagggaatct
     1861 cctcgcacat ctcgcacggc acattacgcg ccgtgaacac cggcgtgccg gtggccaggc
     1921 cgtcgcccgg cgtggccagg cgcaaggtgc cgtaggggca ggcctggacg cacagcccgc
     1981 aacgcaggca ggcgccggcg aactcggctt ccggcagggc gccgggcggg cgcagcgcct
     2041 gcgccggacg ggccgccgcc ggcggcatgg cgaacagcga acccagcgcc agcgcacctg
     2101 cgccgcccgc tgcggcggcg atgcggttga ggaatttccg gcgcgcctgg gaggcaagcg
     2161 cgccggtttc ctttctctgc tggtgggtgg gttcgggttt cacggtggat t
//
LOCUS       DAOSDX010000494         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17074_length_2211_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOSDX010000494 DAOSDX010000000
VERSION     DAOSDX010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17074_length_2211_cov_3.000000"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /gene="flgK"
                     /locus_tag="PLN02_10645"
     CDS             complement(<1..1036)
                     /gene="flgK"
                     /locus_tag="PLN02_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOI52825.1"
                     /translation="MATGLFSIGVSGIKVTQMGLLATEHNIVNANTAGYSRQMTVQST
                     SGAVYTGQGAIGQGVEINTVKRMYDNYLSGHVNSAQSSVGELEALHTQLSQIDNLFAD
                     SSAGLSPALQSFFTGVQSVASDPSSAPARQSMVSSAQTLVSRFDLLNTRLSDMSEQIN
                     SRIENAVTTINSYAKEIASFNQRIVAAQGAYGQPANDLLDQRDQVINELNKLIKVTPT
                     DNGDGSVNLFFGNGQQLVVGTSAMTLTAQPSQADASRTVVGLQGKNVSLELPESSIVG
                     GELGGLLEFRSGSLDKTKAELGMIATSLALTFNAQHALGQDMLGNIAADGGSLQSDFF
                     TLGTPSVLANA"
     gene            complement(1090..2052)
                     /gene="flgJ"
                     /locus_tag="PLN02_10650"
     CDS             complement(1090..2052)
                     /gene="flgJ"
                     /locus_tag="PLN02_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HOI52826.1"
                     /translation="MSIKVQNTPNRAVFDVGATQDLREQLRKDPQAGLKAAAQQFETL
                     FLQMVLKSMRDTVPSDGLLQSDQTRFYNSLLDQQMAQNMASSGNGVGFARLIEQQLGR
                     QYAPTDGAAAGDDLSAILAGKDLPAATNLQYLEVPSRLPTSAAYPAPAASSAAASGSK
                     ATAATTPKEFVSQVWPHAVEASRATGIPPQFLVAQSALESGWGKNEIRKADGTSSYNL
                     FGIKAGAGWTGDSVAVTTTEYVDGKPQQVVENFRAYSSYAEAFRDYAGLLRNSSRYGG
                     VIGTQDGTEFARRLQAAGYATDPKYAEKLASIINGPTLRQALIG"
     gene            complement(2062..>2211)
                     /locus_tag="PLN02_10655"
     CDS             complement(2062..>2211)
                     /locus_tag="PLN02_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOI52827.1"
                     /translation="GSLVSVKAGTSLADVVKALNALGANPMDLLAILQAMKAAGALRA
                     ELEVI"
BASE COUNT          387 a          703 c          689 g          432 t
ORIGIN      
        1 tggcattggc cagcacactc ggcgtgccca gcgtgaagaa gtcgctttgc aggctgccac
       61 cgtcggcggc gatgttaccc agcatgtcct ggcccagcgc gtgctgggca ttgaaagtca
      121 gcgccagcga agtggcgatc atgcccagtt ccgccttcgt cttgtcgagc gaaccactgc
      181 ggaattccag caggccgccg agttcaccgc cgacgatcga cgactcgggc agctccagcg
      241 agacgttctt gccctgcaga ccgaccacgg tccgcgacgc atccgcctgt gaaggctgtg
      301 cggtcaaggt catcgcgctg gttccaacca ccagttgctg accgttgccg aaaaagaggt
      361 tgaccgaacc atcgccattg tcggttgggg tgaccttgat cagcttgttc aattcgttga
      421 tcacctggtc gcgctggtcc agcaaatcgt tggccggctg cccgtaggcg ccctgcgccg
      481 cgacgatgcg ctggttgaag gaagcgattt ccttggcgta gctgttgatc gtcgtaaccg
      541 cattctcgat ccgactgttg atctgctcgg acatgtccga aagacgggtg ttcagcagat
      601 cgaagcggga aaccagagtc tgggcggaac tgaccatcga ctgccgggcc ggcgccgacg
      661 acggatcgga agccaccgac tgtacaccgg taaagaagct ctgcaaggcc ggcgacagac
      721 cggcgctgga atctgcaaac aggttgtcga tctgcgatag ctgggtgtgc agtgcttcga
      781 gttcgccgac actggactgc gcggaattga catgaccgga caggtaattg tcgtacatcc
      841 gcttgaccgt gttgatctcg acgccctggc cgatagcgcc ctgcccggtg tacaccgcgc
      901 cgctcgtcga ctgcaccgtc atctggcgcg aatagccggc ggtgttcgcg ttgacgatat
      961 tgtgctcggt cgccagcaga cccatttgcg tcaccttgat gccgctgacg ccgatactga
     1021 aaagtcctgt tgccatcttt cacctctagg aaggtctacg gcctcccgac cagaaagtta
     1081 aggcgggtat caaccgatca gtgcttgccg gagcgtcgga ccgttgatga tgctggccag
     1141 cttctcggcg tatttgggat cggtggcata gcctgccgcc tgcaaccttc tcgcaaactc
     1201 cgtgccatcc tgggtgccaa tgacaccacc gtaacgcgaa gaattgcgca gcaggccggc
     1261 gtaatcgcgg aaggcctcgg cgtaggacga ataagcccgg aaattctcga cgacctgttg
     1321 cggcttgccg tcaacgtact cggtcgtcgt caccgccacg ctatcgccgg tccagccggc
     1381 cccggccttg atgccgaaga ggttgtacga ggaagtcccg tccgccttgc ggatctcgtt
     1441 cttgccccag ccgctctcga gcgccgactg ggccaccagg aactgcggcg ggatgccggt
     1501 ggcgcgggaa gcctcgaccg catgcggcca gacctgactg acaaactcct tgggcgtcgt
     1561 cgctgccgtc gccttgctgc cggacgcggc cgcgctgctg gcagcgggcg ccggataggc
     1621 cgccgaggtc ggcaagcggc tcggcacttc gagatactgc agattcgtcg ccgccggcaa
     1681 gtccttgccg gcaaggattg ccgacagatc gtcgccggct gccgcgccgt cggtcggcgc
     1741 gtactgacgg cccagttgct gttcgatcag ccgcgcgaag ccgacaccat taccggacga
     1801 cgccatgttc tgggccatct gctgatcgag caggctgttg tagaaacgtg tctggtcact
     1861 ttgcagcaag ccgtcgctgg gtacggtatc gcgcatgctc ttgaggacca tctgcaggaa
     1921 cagggtctcg aattgctgcg ccgccgcctt cagcccggcc tgcgggtcct tgcgcaactg
     1981 ctcgcgcagg tcctgcgtcg ctcccacatc gaaaaccgcg cgattgggcg tgttctgaac
     2041 cttgatcgac atggcggcgg ctcagatcac ttccagttcg gcacgtagcg cgccggccgc
     2101 cttcatcgcc tggagaatgg ccagcaagtc catcggattg gcgcccagcg cgttgagcgc
     2161 cttgaccacg tcggccaggc tggtgccggc cttgacgctg accaggctgc c
//
LOCUS       DAOSDX010000495         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17128_length_2205_cov_3.791163, whole genome shotgun sequence.
ACCESSION   DAOSDX010000495 DAOSDX010000000
VERSION     DAOSDX010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17128_length_2205_cov_3.791163"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..611)
                     /gene="moaE"
                     /locus_tag="PLN02_10660"
     CDS             complement(156..611)
                     /gene="moaE"
                     /locus_tag="PLN02_10660"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HOI52828.1"
                     /translation="MTVRVQTGDFDVGAELAALRAGDARVGALASFLGLVRDINDGAS
                     VAGMTLEHYPGMTEKALAQIVAEAKSRWNIYDALVIHRVGPLAPCDQIVLVAVTSAHR
                     GEAFAACEFIMDYLKTKAPFWKKEATADGGRWVDARDTDDSAAARWQQP"
     gene            complement(613..2187)
                     /locus_tag="PLN02_10665"
     CDS             complement(613..2187)
                     /locus_tag="PLN02_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOI52829.1"
                     /translation="MSQLEQEISRRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEV
                     RARKASRHATSDWMELEKQRGISVTSSVMQFPYRECMINLLDTPGHEDFSEDTYRTLT
                     AVDSAVMVIDSVNGVEAQTIKLLNVCRMRDTPILTFINKLDREGKEPIDLLDEIESVL
                     GIQCAPMTWPIGMGKRFRGVYHLYDDTIAFFDPQAEKGTAEMIPGLDNPRLDELIGSQ
                     ADELRSDIELVRGASHPFDAEAYLSGKQCPVFFGSAVNNFGVQSLLDAVVDLSPAPKA
                     RPAVTRTVDAFEPKFSGFVFKIQANMDPKHRDRIAFLRVCSGRFDRGMKVKQVASGKT
                     LSINNAITFMARDRSTADEAWSGDIIGIPNHGTIRLGETFTEGEDLRFTGIPSFAPEH
                     FRLARIANPLKIKQLQKGLQQLAEEGATQLFRPLSGTDLILGAVGTLQFDVVASRLEN
                     EYGVQVIFESYNCSTARWIHGDPGELKQLSERYSANVALDGADDPVYLAPNNVYLNMV
                     KEKYPKLRFDEAREVA"
BASE COUNT          340 a          691 c          757 g          417 t
ORIGIN      
        1 gcacagaccg agtgggcgct ggcgctgcac ggactgggca gccagatggg cggcttgcag
       61 gcgccgctga cgctggcggc ggccgaactc ttcttctggc tggccatcgc cgtcctgccg
      121 gtcgccgcgc tggtccggct gcagcggacc tttcgttaag gctgctgcca gcgcgccgcc
      181 gcgctgtcgt cggtgtcgcg ggcatcgacc cagcgcccgc cgtccgccgt cgcttccttc
      241 ttccagaacg gcgctttcgt cttcaggtag tccatgatga attcgcaggc ggcgaaggct
      301 tcgccgcgat gcgcgctggt caccgcgacc agcacgatct ggtcgcaggg cgcgagcggg
      361 ccgacgcggt ggatgaccag cgcgtcgtag atgttccagc gcgatttcgc ctcggcaacg
      421 atctgtgcga gcgccttctc ggtcatcccc gggtaatgct ccagggtcat cccggcaacg
      481 ctggcgccgt cgttgatgtc gcgcactagg ccaaggaagg aagcgagcgc gccgacccgg
      541 gcgtcgcccg cgcgcagcgc cgccagttcg gcgccgacgt cgaaatcccc ggtctggacg
      601 cggaccgtca tgtcaggcca cctcgcgcgc ttcgtcgaag cgcagcttgg ggtatttttc
      661 cttgaccatg ttcagataca cgttgttcgg cgccaggtag accggatcgt cggcgccgtc
      721 gagggcgacg ttggcgctgt agcgctcgga caactgcttg agttcgccgg ggtcgccgtg
      781 gatccagcgt gccgtcgaac aattgtagct ttcgaagatg acctgcacgc cgtactcgtt
      841 ttccagccgg ctggcgacga cgtcgaactg cagcgtgccg accgcgccga ggatcaggtc
      901 ggtgcccgac agcggacgga acagctgggt cgcgccctct tccgccagtt gctgcaggcc
      961 cttctgcaac tgcttgatct tcagcgggtt ggcgatccgc gccaaacgga agtgttcggg
     1021 cgcgaacgac ggaatgccgg tgaagcgcaa atcctcgccc tcggtgaagg tctcgccgag
     1081 gcggatcgtg ccgtggttgg gaataccgat gatgtcgccc gaccaggctt cgtcggcggt
     1141 cgaccggtcg cgcgccatga aggtgatcgc gttgttgatc gacagcgtct tgccgctcgc
     1201 cacctgcttg accttcatgc cgcggtcgaa gcggccggag cagacgcgca ggaaggcgat
     1261 ccggtcacgg tgcttggggt ccatgttggc ctggatcttg aacacgaagc cggagaactt
     1321 gggctcgaag gcgtccaccg tccgcgtcac cgccggccgt gccttgggcg ccggcgacag
     1381 gtcgacgacg gcatcgagca ggctctgcac gccgaagttg ttgaccgccg agccgaagaa
     1441 caccgggcac tgcttgccgg aaagataggc ctcggcgtcg aaggggtgcg acgcgccgcg
     1501 caccagttcg atatcgctgc gcagttcgtc ggcctggctg ccgatcagct cgtcgaggcg
     1561 cgggttgtcg aggcccggga tcatttccgc cgtgcccttc tcggcctgcg ggtcgaagaa
     1621 ggcgatggta tcgtcgtaga ggtgatagac gccgcggaaa cgcttgccca tgccgatcgg
     1681 ccaggtcatc ggtgcgcact ggataccgag caccgactcg atttcgtcga gcaggtcgat
     1741 cggctccttg ccttcacggt cgagcttgtt gatgaaggtc aggatcggcg tgtcgcgcat
     1801 gcggcagacg ttcaagagct tgatcgtctg cgcttcgacg ccattgaccg agtcgatcac
     1861 catgaccgcc gagtcgacgg cggtcagcgt gcggtaggtg tcttccgaga agtcctcgtg
     1921 gcccggcgtg tcgagcaggt tgatcatgca ttcgcggtac gggaactgca tgaccgacga
     1981 ggtgaccgag atgccgcgct gcttttccag ttccatccag tccgacgtcg catggcggct
     2041 ggccttgcgg gcgcggactt cgccggcgac ctggatggcg ccgccgaacc acagcagttt
     2101 ttcggtcagc gtggttttac ccgcgtcggg gtgggaaatg atcgcgaagg tgcgacggcg
     2161 ggaaatttct tgttcgagct ggctcacggc gtttcggaaa ggcgc
//
LOCUS       DAOSDX010000496         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17135_length_2204_cov_3.219637, whole genome shotgun sequence.
ACCESSION   DAOSDX010000496 DAOSDX010000000
VERSION     DAOSDX010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17135_length_2204_cov_3.219637"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLN02_10670"
     CDS             <1..544
                     /locus_tag="PLN02_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOI52830.1"
                     /translation="NGPKGAAALLVDKRIELQPQIAGGGHERGLRSGTENAPAIVGFG
                     VAAELAAQRVAQSAARLAALKDSLERGLAALGASIFAVDAPRLPNTSYFAIAGIDGET
                     LVGKLDREGFAVASGAACSSANPEPSHVLRAMGVAPEIARGAVRVSLGNDNGDADVEQ
                     FINALQVTVGRLQGLTAVTA"
     gene            566..1777
                     /locus_tag="PLN02_10675"
     CDS             566..1777
                     /locus_tag="PLN02_10675"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOI52831.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMIPYLCEHFGNPASRSHSFGWV
                     AEAAVEEAREQVAALVGADPKEIVWTSGATESNNLAIKGAANFYAATKGKHIITVKTE
                     HKAVLDTVREMERQGFEATYLDVQENGLVDLEAFKAAIRPDTVLASVMFVNNEVGVVQ
                     PIAELGEICRAKGVIFHVDAAQATGKVDIDLGQLKVDLMSFSAHKTYGPKGIGALYVR
                     RKPRIRLEAQMHGGGHERGFRSGTLATHQIVGMGEAFRIAREEMAEENKRVKGLRDKL
                     MKGLQDIEATFVNGDLEQRVAHNLNISFAYVEGESMIMAIKDLAVSSGSACTSASLEP
                     SYVLRALGRDDELAHSSIRFSIGRFTTEEEIDFAVKLLREKIQKLRDLSPLWDMYKEG
                     IDLSTVQWAAH"
     gene            1798..2181
                     /gene="iscU"
                     /locus_tag="PLN02_10680"
     CDS             1798..2181
                     /gene="iscU"
                     /locus_tag="PLN02_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOI52832.1"
                     /translation="MSYSVKVIDHYENPRNVGSLDKDDSDVGTGMVGAPACGDVMKLQ
                     IKVNKAGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTVDQALGIKNTDIAEELALPPV
                     KIHCSILAEDAIKAAVADYKKKRGE"
BASE COUNT          462 a          696 c          684 g          362 t
ORIGIN      
        1 caacggcccc aagggggcgg cggccttgct cgtcgacaag cgcatcgaac tgcagccgca
       61 gattgccggt ggcggtcacg agcgcggcct gcgctcgggg accgagaacg cgccggcgat
      121 cgtcggcttc ggcgtcgccg cggaacttgc ggcgcagcgc gttgcccaat cggcggcgcg
      181 tctggccgcc ctgaaggata gtctcgagcg cgggctggcc gcacttgggg cgagcatttt
      241 cgcggtcgac gcgccacgtc tgccgaacac cagttatttc gcgattgccg gcatcgacgg
      301 cgaaaccctg gtcggcaagc tcgaccggga aggttttgcc gtcgccagcg gcgccgcctg
      361 ctccagcgcc aacccggaac cctcgcacgt gctgcgggcg atgggtgtgg ccccggaaat
      421 cgcccgcggc gcggtgcgcg tcagcctagg taacgacaac ggcgatgccg atgtcgaaca
      481 attcatcaac gccttgcagg tcacggtcgg acgcctgcag ggattgacgg cggtcaccgc
      541 ctgaaccgac ccagcgagaa agacgatgaa actgcctatc tacctcgatt actcggccac
      601 caccccggtc gatccgcgcg ttgccgaaaa gatgattccc tacctgtgcg agcatttcgg
      661 caatcccgca tcgcgttcgc acagtttcgg ctgggtggcc gaggccgccg tcgaggaggc
      721 gcgcgagcag gtcgccgcgc tggtcggcgc cgatcccaag gaaatcgtct ggacttccgg
      781 cgccaccgaa tcgaacaacc tggcgatcaa gggcgccgcc aatttctacg cagccaccaa
      841 gggcaagcac atcatcaccg tcaagaccga gcacaaggcg gttctcgaca ccgtgcgcga
      901 gatggagcgg cagggcttcg aggcaaccta cctcgacgtc caggaaaacg gcctcgtcga
      961 tctcgaagcc ttcaaggcgg cgatccgccc ggacaccgtg ctggcgtcgg tgatgttcgt
     1021 caataacgaa gtcggcgtcg ttcagccgat cgccgaactc ggcgaaatct gccgcgccaa
     1081 gggcgtcatc ttccacgtcg acgcggcgca ggcgaccggc aaggtcgaca tcgacctggg
     1141 ccagctcaag gtcgacctga tgagcttctc ggcccataag acctatggcc cgaagggcat
     1201 cggcgccctc tacgtgcggc gcaagccgcg catccggctg gaagcgcaga tgcacggcgg
     1261 cggtcacgag cgcggtttcc gctccggcac gctggccacg caccagatcg tcggcatggg
     1321 cgaagccttc cgtatcgcgc gcgaggaaat ggccgaagaa aacaagcgag tcaaaggctt
     1381 gcgcgacaaa cttatgaaag gacttcaaga tattgaagcg actttcgtca atggcgacct
     1441 ggaacagcgc gtcgcccaca acctcaacat cagcttcgcc tacgtcgaag gcgagtcgat
     1501 gatcatggcg atcaaggacc tcgcggtgtc gtccggttcg gcctgcacct cggccagcct
     1561 ggagccgtcc tacgtactgc gcgcgctggg gcgcgacgac gaactggcgc acagctcgat
     1621 ccgtttctcc atcggtcgct tcaccaccga agaagaaatc gactttgcgg tcaagctctt
     1681 gcgcgagaag attcagaaac tacgtgacct ttccccgctg tgggacatgt acaaggaagg
     1741 tatcgatctg agtaccgtcc agtgggctgc ccactaacga attaacggag aaataacatg
     1801 agctatagcg taaaagtcat tgatcactat gagaatcctc gcaacgtcgg ctcgctcgac
     1861 aaagacgatt ccgatgtcgg caccggcatg gtcggcgccc cggcctgcgg cgacgtgatg
     1921 aagctgcaga tcaaggtcaa caaggctggc gtcatcgaag acgccaagtt caagacctac
     1981 ggttgcggct cggcgatcgc ctcgtcgtcg ctggttaccg agtgggtcaa gggcaagacc
     2041 gtcgatcagg cgctgggcat caaaaatacc gacattgccg aggaactggc gttgccgccg
     2101 gttaaaatcc actgctcgat tctggccgaa gacgccatca aggcggcagt ggccgattac
     2161 aagaaaaaac gcggagagta agcatggccg tgaccctgac cgac
//
LOCUS       DAOSDX010000497         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17151_length_2202_cov_3.887285, whole genome shotgun sequence.
ACCESSION   DAOSDX010000497 DAOSDX010000000
VERSION     DAOSDX010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17151_length_2202_cov_3.887285"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2202)
                     /locus_tag="PLN02_10685"
     CDS             complement(<1..>2202)
                     /locus_tag="PLN02_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOI52833.1"
                     /translation="APPSEPIVTILEDANNDTWINAAELQGNIDVSVTLPATAVAGDT
                     LLTTVNGVAQAPITITQADILKGSIALPGVASPGDGVKLTVDAQIKDAAGNVGKTGSD
                     SATIDTSIPNDGNPPTVIITEDANNDALISAPELSGNVDVRIEFDGSKVEVGDTLRVS
                     DGVNTKDFVISAADKANGYITTDFAARPAGETITVDAYMFDPAGNVGKTGSDQARFAL
                     IQLGDLLATVSEEGLAGGIKDSSGNPGDVADSATASGNIGVTGAGGASLLVSVAPPSE
                     ALSADGVQIVWSGSGSSASPLIGTAGQGGPEVLRVTVDSNGNYEIKLSQAIDHAAGQD
                     ENVETLHFDLTADDGLSSTQAALTLDIEDDAPKAVPETEADAMRHQDTNILLILDNSG
                     SMAGSRIEILRNSVRELIQSYDMLGDVAVRIVVYSSSAKSYTEGWVSAADAIAYVNAL
                     GTGGATNYDAALLKAMEAFQSSGKIPDGTNVAYFLSDGEPNASSDWDGSGPLPARQGI
                     QAEEEALWKSFLETNKINAFAFGMGSDTTQASMNPVAYNGVTKTDTDAVIVANESELG
                     PILRDTVNANFKGNFLDGTLHQGSGVGADGGHLTSFSIDGTTYLLDGTVSGTAIGTAD
                     KFDGQKWTVFTDAGGKLVVDMLSGEYVYTPPIKSNATTEQIGFAIIDNDGDHAASTLS
                     LTVTPPPAKIFGGSGDDTLNGGNYGDYIDGKEGNDLIVGGQGDDTLIGGLGA"
BASE COUNT          308 a          637 c          792 g          465 t
ORIGIN      
        1 ggcaccgaga ccgccgatca gggtgtcgtc gccctggccg ccgacgatca ggtcgttgcc
       61 ttccttgccg tcgatatagt cgccatagtt gccgccattg agcgtgtcat cgccgctgcc
      121 accgaagatc ttggctggcg gcggagtgac cgtcagcgat agcgtcgagg cagcgtggtc
      181 gccgtcgttg tcgatgatgg cgaagccgat ctgctcggtg gtggcgttgc tcttgatcgg
      241 cggcgtatag acgtattcgc cgctgagcat gtcgaccacc agcttgccgc cggcatcggt
      301 gaataccgtc cacttctggc cgtcgaactt gtcggcggtg ccgattgccg taccgctgac
      361 ggtgccgtcg agcagatagg tggtgccgtc gatgctgaac gaggtcagat gcccgccgtc
      421 ggcaccgacc ccggagccct ggtgcagggt gccgtcgagg aagttgccct tgaaattggc
      481 attgacggta tcgcgcagga tggggccaag ctcgctttcg ttggcgacga tgacggcgtc
      541 ggtatcagtc ttggtgacac cgttgtaagc gaccggattc atgctggcct gggtggtgtc
      601 gctgcccatg ccgaaggcga aggcgttgat cttgttggtt tcgaggaagg acttccagag
      661 cgcttcttct tccgcctgaa tgccttggcg cgccggcagc ggaccgctgc cgtcccagtc
      721 gctggaagcg ttcggttcgc cgtcggacag gaagtaggcg acattggtgc cgtcagggat
      781 cttgccggac gactggaagg cttccatggc ttttagcagg gccgcgtcgt agttggtggc
      841 accgcctgtg cccagggcat tgacgtaggc gatggcgtcg gcggcactga cccagccttc
      901 ggtataagac ttggccgagg agctatagac gacgatgcgg acggcaacgt cgccgagcat
      961 gtcgtaactc tgaatcagtt cgcgcaccga gttgcgcagg atctcgatgc ggctgccggc
     1021 catgctgccc gaattgtcga ggatgagcag gatgttggtg tcctgatggc gcatggcgtc
     1081 ggcttcggtt tccggaactg ccttcggcgc atcgtcctcg atgtccagcg tcagggccgc
     1141 ctgggtgctg ctcaagccat cgtcggcggt gaggtcgaag tgcagggttt cgacgttttc
     1201 gtcctggccg gccgcgtggt cgatggcttg cgacagcttg atttcgtagt tgccgttgct
     1261 gtcgaccgtg acccgaagca cttcggggcc gccctggccg gccgtgccga tcagcggtga
     1321 agcactgctg ccgctgcccg accagacgat ctggacgccg tcggcgctca gggcttcgct
     1381 cggtggcgca acgctgacca gcagggaggc gccgccggca ccggtgacgc cgatgttgcc
     1441 gctggccgtc gcgctatcgg caacatcgcc cgggttgccg ctgctgtcct tgatgccgcc
     1501 ggccagtccc tcttcggaaa cggtggcgag caggtcgccg agctggatca gggcaaagcg
     1561 ggcctgatcg ctaccggttt tgccgacgtt gccggccggg tcgaacatgt aggcgtcgac
     1621 ggtgatggtt tcgccggcgg ggcgcgccgc gaaatcggtg gtgatgtagc cgttcgcctt
     1681 gtcggcagcg ctgatgacga agtccttggt gttgacgccg tcggagacgc gcaacgtatc
     1741 gccgacctcg accttggaac cgtcgaattc gatcctgacg tcgacgttgc cgctgagttc
     1801 cggcgcgctg atcagggcgt cgttgttggc gtcctcggtg atgatgacgg tgggcggatt
     1861 gccgtcgttg gggatgctgg tgtcgatggt cgcgctgtcg ctgccggttt tgccgacgtt
     1921 gccggcggcg tccttgatct gggcgtcgac ggtgagcttg acgccatcgc cggggctggc
     1981 gacgccgggc agggcgatcg agcccttgag gatgtcggcc tgggtgatgg tgatcggcgc
     2041 ctgggccacg ccattgaccg tggtcagcag ggtgtcgccg gcaacggcgg tggccggcag
     2101 ggtgacgctg acgtcgatgt tgccctgcag ttcggcggcg ttgatccagg tgtcgttgtt
     2161 ggcgtcttcg aggatggtga cgatcggctc gctgggcggc gc
//
LOCUS       DAOSDX010000498         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17178_length_2199_cov_2.487407, whole genome shotgun sequence.
ACCESSION   DAOSDX010000498 DAOSDX010000000
VERSION     DAOSDX010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17178_length_2199_cov_2.487407"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PLN02_10690"
     CDS             complement(<1..133)
                     /locus_tag="PLN02_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOI52834.1"
                     /translation="MAEVRNADTRERILDAAERLFMAYGYDGTSMRQITGEASVNLAA
                     "
     gene            complement(214..>2199)
                     /locus_tag="PLN02_10695"
     CDS             complement(214..>2199)
                     /locus_tag="PLN02_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI52835.1"
                     /translation="PTGRLTWSEELYRIFGIDPQAGPGDYQRFLQMVPAEDRERVDEA
                     YRASFREREPHEVEHRLITGSGQIKQIRSRWETHFDALGNPLRTIGTAQDITDLRRME
                     SQMQLLGTAFHHSGEAILITDHDNRIVTVNPAFTTLTGYTLEDVAGKNPKLLSAGRTL
                     PEEYERMWASIRQKGYWQGEVWDRRKDGGVYPKWLSISVMRDDAGEIRYHIAHFTDVS
                     AERAAEARLHHMAHHDMLTGLANRFSLKERLEHALAVARRESSRVALLFIDLDRFKVI
                     NDTLGHHIGDELLIQVSQRLRSAVRDSDLVARLGGDEFVVMASGVEQMSSVATVAEKL
                     VKVVGEPYAIGPHTLYTTPSIGIAVYPMDGENGEALMKNADAAMYHAKAAGRNNFQFF
                     DARMNEAAIERLNIEHALHQALERDEFVLYYQPVIDLGSGRVSCVEALIRWRHPERGL
                     VAPGLFIGVAEETGLIQPIGDWVIWSACRQLAAFRAAGIADVKMAINISAVQMRNSSL
                     PVVARGVIDAYSLARGDLIFEITESVAMEQPDETIRVLDVLRSMGVTLALDDFGTGYS
                     SLSYLRMFALDKLKLDRSFVEGIGEDIDGQVICDATIGLAHALGLTLVAEGVETDAQL
                     DYLRARGCDLVQGYLFSKPLPALEAEAFIRQRNGSPG"
BASE COUNT          406 a          703 c          662 g          428 t
ORIGIN      
        1 ccgccgccaa attgacgctg gcttcgccgg tgatctggcg catcgacgta ccgtcgtagc
       61 cgtaggccat gaacaggcgc tcggcggcgt cgaggatgcg ctctcgcgta tcggcattgc
      121 gaacttcagc cattcttgct tcctatgatt catacgttcg tttgaatcat aggaaaaggc
      181 gcccgccttg gcaagccttt tctcttacga aagttagcct ggcgagccgt tacgctggcg
      241 gatgaaagct tccgcctcga gcgcgggcaa tggtttgctg aacaggtaac cctgcaccag
      301 atcacagccg cgagcccgca gatagtcaag ctgggcgtcg gtctcgacgc cttcggcgac
      361 cagcgtcagg cccagggcgt gggcgagacc gatcgtcgcg tcgcagatga cctgaccgtc
      421 gatgtcctca ccgataccct cgacgaagga acgatccagt ttcagcttgt ccagggcgaa
      481 catgcgcagg tagctgagcg aggaatagcc ggtcccgaaa tcgtcgagcg ccaacgtcac
      541 ccccatgctg cgcaggacat caaggacacg gatggtttcg tccggctgct ccatcgccac
      601 cgattcggtg atttcgaaga tgagatcgcc gcgggccagc gagtaggcat caatgacccc
      661 acgggcgaca accggcaggc tgctgttacg catctgcacc gccgagatgt tgatcgccat
      721 cttgacatcg gcgatgcccg ccgcgcgaaa ggcggcgagt tggcgacagg cgctccagat
      781 cacccagtcg ccgatcggct ggatcaggcc ggtttcctcg gcgacgccga tgaacaaacc
      841 cggcgccacc agtccacgtt ccgggtgccg ccagcggatc agggcttcga cgcaggatac
      901 ccggccgctg ccgaggtcga tcaccggctg gtagtacaac acgaattcgt cgcgttcgag
      961 cgcctgatgc aaggcgtgtt cgatgttcag gcgttcgatc gccgcttcgt tcatgcgcgc
     1021 gtcgaagaac tggaagttgt tccgcccggc cgccttggcg tggtacatcg cggcgtcggc
     1081 gttcttcatc agcgcttcgc cattctcgcc gtccatcgga taaacggcga tgccgatact
     1141 cggcgttgta tatagcgtgt gcgggccgat cgcatagggt tcgccgacga ccttgacgag
     1201 tttctcggcg accgtcgcca ccgaactcat ctgctccaca ccactcgcca tgacgacgaa
     1261 ttcgtcgccg ccaagacggg caaccagatc gctgtcgcgc accgcgctgc gcagtcgctg
     1321 gctgacctgg atcagcagtt cgtcgccgat gtgatgcccc agcgtgtcgt tgatcacctt
     1381 gaagcgatcg agatcgatga acagcagcgc gacccgcgag ctttcccggc gtgccaccgc
     1441 cagcgcgtgc tcgagacgtt ccttcaggct gaagcgattg gccaacccgg tcagcatgtc
     1501 gtggtgcgcc atgtgatgga ggcgcgcttc ggccgcgcgc tctgccgaga catcggtgaa
     1561 atgggcgatg tgatagcgga tttcaccggc atcgtcgcgc atcaccgaaa tcgacagcca
     1621 tttcggatag accccgccat ccttgcgccg atcccagacc tcgccttgcc agtagccctt
     1681 ctgcctgatc gacgcccaca tccgctcata ctcctcgggc aaagtccgac cggccgacag
     1741 caacttggga ttcttcccgg ccacatcttc cagcgtgtag ccggtcaagg tggtgaacgc
     1801 cgggttgacg gtgacgatgc gattgtcgtg atcggtgatc aggatggctt cgccgctgtg
     1861 gtggaaagcc gtgcccagca actgcatctg cgattccatg cgccgcaggt cggtaatgtc
     1921 ctgcgcggta ccgatcgtgc gcagcggatt gcccagcgca tcgaagtggg tttcccaacg
     1981 cgaacgaatc tgcttgatct gaccgctgcc ggtgatcagt cgatgctcca cctcgtgcgg
     2041 ttcgcgttcc cggaacgatg cacggtaggc ttcgtcgacg cgttcgcgat cctcggccgg
     2101 gaccatctgc aggaaacgct gataatcacc cggccccgcc tgcggatcaa tgccgaaaat
     2161 ccggtagagt tcttccgacc aagtcagacg gccggtcgg
//
LOCUS       DAOSDX010000499         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17226_length_2195_cov_3.700000, whole genome shotgun sequence.
ACCESSION   DAOSDX010000499 DAOSDX010000000
VERSION     DAOSDX010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17226_length_2195_cov_3.700000"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLN02_10700"
     CDS             complement(<1..570)
                     /locus_tag="PLN02_10700"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HOI52836.1"
                     /translation="MDSLTLGILIAAAIIAGFLLIVARRGKAAGPADLPSPRQEAAAR
                     LEPRPARNADPETRFIESPKRKQYRLLNDSEQELYHRLCEAVPNLRVFAQVGVAQLAL
                     ARGRQEAQRLNRMAGRGVDFVICDEDFSIVAAIELAWPTSAQETDNSEEVKRVALQSL
                     GVPLIVLRPNKLPEADTISREIADAILRRK"
     gene            664..1395
                     /locus_tag="PLN02_10705"
     CDS             664..1395
                     /locus_tag="PLN02_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOI52837.1"
                     /translation="MELIFSPLGWLILAAFVAGLVDAVVGGGGLIQIPALFAAQPGVA
                     PATLFGTNKFASVFGTTNAAWRYARRVDMPWRTILPAAAAAFVFSYAGAAAVAWLPKE
                     AVRPLILVLLIFSAAYTLWKKDFGQLHRPAHSGHRELLYAVLLGGVIGFYDGFFGPGT
                     GSFLIFLFIRFFGFDFLHASAGAKVVNVATNVAALAFFVPAGHLLLGLAVGMALANVA
                     GSATGTWLALRHGSGFSKDEDEACG"
     gene            1379..>2195
                     /locus_tag="PLN02_10710"
     CDS             1379..>2195
                     /locus_tag="PLN02_10710"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOI52838.1"
                     /translation="MKRVDDFRLRFGKHELVPIMIGGMGVDISTADLALEAARLGGVG
                     HISDAMINTVSDRRYDTKHVKEKLALYKYNVNNEDKSSVKFDLGRLEEATRLHVEATM
                     TRKQGSGMVFINCMEKLTMNAPRETLKVRMSAALDAGIDGITLAAGLHLGSFALIEDH
                     PRFRDAKLGIIVSSVRALQLFLKKSARTNRLPDFIVVEGPLAGGHLGFGMDWAQYDLA
                     TIVAEVMLYLKNEHLDIPVIPAGGIFTGTDAAQFLDAGAAAVQVATRFTVAEEC"
BASE COUNT          346 a          716 c          697 g          436 t
ORIGIN      
        1 tttgcggcgc aggatggcgt cggcgatttc acggctgatg gtatcggctt ccggcagctt
       61 gttgggacgc aagacgatca gcggaacgcc cagactctgc agcgcgacac gcttcacttc
      121 ttccgaattg tcggtttcct gggcgctggt cggccaggcc agctcgatgg cggcgacgat
      181 cgagaaatcc tcgtcgcaga tcacgaaatc gacgccgcgt ccggccatcc ggttcaggcg
      241 ctgcgcttcc tggcggccgc gcgccagcgc cagctgggca acgccgacct gggcgaagac
      301 gcgcaggttg ggcacggctt cgcacaagcg gtggtagagt tcctgctcgc tgtcgttgag
      361 caggcggtat tgcttgcgtt tcggcgattc gatgaagcgg gtttccggat cggcgtttct
      421 tgccggtcgc ggttcaaggc gcgcggcggc ttcctggcgg ggtgacggca aatcggccgg
      481 gccggcggcc ttgccgcgac gtgcgacaat cagcagaaag ccggcaatga tggcggcggc
      541 gatcaggatg cccagagtca gagagtccat gcaacgtatc cttggttcga tgccgcatta
      601 taacgacatg gcatcctgcc tgatcacccc gggcgtagct cattcccctt tcgcagaatt
      661 ctcatggaac tgattttttc cccgctcggc tggctcatcc tggccgcctt cgtcgccggc
      721 ctggtcgatg ccgtggtcgg cggcggcggc ctgatccaga tcccggcgct gtttgccgcc
      781 cagcccggcg tggcgccagc gacgctgttc ggcaccaaca agtttgccag cgtcttcggt
      841 acgactaatg ccgcctggcg ctacgcccgc cgggtcgaca tgccgtggcg caccatcctg
      901 ccggcggcgg ccgctgcctt cgtcttttcc tatgccggcg cggcagcggt cgcctggctg
      961 cccaaggaag cggtgcggcc gctgatcctg gtgctgctca tcttctctgc cgcctacacc
     1021 ctgtggaaaa aggatttcgg ccagttgcac cggccagccc acagcggtca ccgcgaactg
     1081 ctctacgcgg tcctgctcgg tggcgtgatc ggtttctacg acgggttttt cggaccggga
     1141 accggcagtt tcctgatttt cctgttcatc cgcttcttcg gcttcgattt cctgcatgcc
     1201 tcggccggcg ccaaggtggt caatgtggcg accaatgtcg ccgccctcgc cttcttcgtg
     1261 ccggccggtc acctcctgct cgggctggcg gtcggcatgg cgctcgccaa tgtcgccgga
     1321 tcggcgaccg ggacctggct ggcgctgcgc catggcagcg gtttttccaa ggatgaagat
     1381 gaagcgtgtg gatgacttcc gcttgcgatt tggcaagcac gagctggttc ccatcatgat
     1441 cggcggcatg ggcgtcgata tctcgacggc cgatctggcc ctggaggccg cccggctcgg
     1501 cggcgtcggc catatttcgg acgcaatgat caacaccgtt tccgaccgcc gttacgacac
     1561 caagcacgtc aaggaaaagc tcgcgctgta caagtacaac gtgaacaacg aggacaagtc
     1621 gtcggtcaag ttcgacctcg gccgtctcga ggaagccacc cgtctgcacg tcgaagcgac
     1681 gatgacgcgc aagcagggca gcggcatggt cttcatcaac tgcatggaaa agctgaccat
     1741 gaacgcgccg cgcgagaccc tcaaggtccg catgtcggcc gcgctcgatg ccggcatcga
     1801 cggcatcacg ctggcggccg gcctgcacct cggctctttt gccctgatcg aggaccaccc
     1861 gcgcttccgc gatgccaagc tgggcatcat cgtttcttcc gtgcgcgccc tgcaactttt
     1921 cctcaagaag agcgcacgca ccaaccgcct gcccgatttc atcgtcgtcg aaggcccgct
     1981 cgccggcggc cacctcggct tcggcatgga ctgggcgcag tacgacctgg cgaccatcgt
     2041 cgccgaggtg atgctgtacc tgaagaacga acatctggac attccggtga ttccggccgg
     2101 cggcatcttc accggtaccg acgcggcgca gttccttgat gccggcgcgg ctgccgtgca
     2161 ggtggcgacg cgtttcacgg tggccgagga atgcg
//
LOCUS       DAOSDX010000500         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17256_length_2192_cov_2.854469, whole genome shotgun sequence.
ACCESSION   DAOSDX010000500 DAOSDX010000000
VERSION     DAOSDX010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17256_length_2192_cov_2.854469"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PLN02_10715"
     CDS             complement(<1..1178)
                     /locus_tag="PLN02_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOI52839.1"
                     /translation="MTTTTFRRDLANALRFLAIDAVEQAKSGHPGAPMGMADIAEVLW
                     RDHLRHNPANPQWADRDRFVLSNGHGSMLLYALLHLSGYDLPLDELKRFRQFGSKTAG
                     HPEVHHTPGVETTTGPLGQGISNAVGMALAEKLLAQRYNRPGHSIVDHRTWVFLGDGC
                     LMEGISHEACALAGVWGLDKLVAFYDDNGISIDGHVEGWFRDDTVARFRAYGWDVIGP
                     LDGHDPVAIAAATAEARQSRGRPTLIVCRTQIGWGSPNKAGTHDVHGAPLGAAEVAAT
                     RAALGWPHAPFEIPEALRAAWDARASGAAAEAAWQQRFAAYRAAYPALAAEFERTQAG
                     GLPEAWPEIRAELLAQAAAKTGGIASRKSSQNCLDFLIERVPELLGGSADLTGSNLTA
                     G"
     gene            complement(1211..2083)
                     /locus_tag="PLN02_10720"
     CDS             complement(1211..2083)
                     /locus_tag="PLN02_10720"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOI52840.1"
                     /translation="MSVKHPIIAITGSSGAGTSTVMQSFQHIFRREKINAQIVEGDSF
                     HRYDRLSMRAEMKAQGEQGNVNFSHFGPEANLLEELQDLFVSYGKNGSGKVRKYLHDA
                     AEAAPWQQEPGTFTPWQDIPAATDLMFYEGLHGAWSGDGIDIAAQVDLCVGVVPIINL
                     EWIQKLHRDQKMRGYSQEAVVDTILRRMPDYVNHICSQFGRTHVNFQRVPIVDTSNPF
                     IARDIPSADESMVVIRFANPKGIDFQYLINMLHGSMMTRPNTLVVPGGKMGLAMQMIF
                     TPMVLRLMDQKRRA"
BASE COUNT          329 a          676 c          806 g          381 t
ORIGIN      
        1 ccggcggtca ggttggagcc ggtgaggtcg gccgaaccgc cgagcagttc gggaacgcgc
       61 tcgatcagga aatcgaggca gttctgcgac gacttgcggc tggcgatgcc gccggtcttg
      121 gctgccgcct gggcgagcag ttcggcgcgg atttccggcc aggcttccgg caggccgccg
      181 gcctgggtgc gctcgaattc ggcggccagc gccggatagg cggcgcggta ggcggcgaag
      241 cgctgttgcc atgcggcctc cgccgccgcg ccgctggcgc gggcgtccca ggcggcgcgc
      301 agcgcctcgg gaatctcgaa cggcgcgtgc ggccagccga gcgcggcgcg ggtggcggcg
      361 acttccgcgg cgccgagcgg cgcgccatgc acgtcgtgcg tgccggcctt gttcggcgac
      421 ccccagccga tctgcgtgcg gcagacgatc agcgtcggcc ggccgcgcga ctggcgggcc
      481 tcggctgtcg ccgcggcgat ggcaaccgga tcgtggccgt cgagcgggcc gatgacgtcc
      541 cagccgtaag cgcggaagcg ggcaacggtg tcgtcgcgga accagccttc gacgtggccg
      601 tcgatggaaa tcccgttgtc gtcgtaaaag gcgaccagct tgtccagccc ccagacgccg
      661 gccagggcgc aggcttcgtg gctgatccct tccatcaggc aaccgtcgcc aaggaagacc
      721 caggtgcggt gatcgacgat gctgtggccg gggcggttgt agcgctgcgc cagcagcttc
      781 tcggcgagcg ccatgccgac ggcgttgctg atgccctggc cgagcggccc ggtggtcgtc
      841 tcgacgcccg gcgtgtggtg gacttccgga tggccggcgg tcttgctgcc gaactggcgg
      901 aaacgcttga gttcgtcgag gggcagatcg taaccgctca ggtgcagcag cgcatagagc
      961 agcatcgagc cgtggccgtt cgacagcacg aagcggtcgc ggtcggccca ctgcgggttg
     1021 gcggggttgt ggcgcagatg gtcgcgccac aagacttcgg cgatgtccgc catgcccatc
     1081 ggcgcgccgg ggtggccgga tttcgcctgc tcgacggcgt cgatggcgag gaaacgcagg
     1141 gcgttcgcca ggtcacggcg gaaggtggtg gtcgtcatgt ctctctccat ggctgaaata
     1201 tccgtccgtc tcaggcgcgc cgcttctggt ccatcagacg caggaccatc ggcgtgaaga
     1261 tcatctgcat cgccagcccc atcttgccgc cgggcacgac cagggtgttc gggcgggtca
     1321 tcatcgagcc gtgcagcatg ttgatcaggt actggaaatc gatccctttc gggttggcga
     1381 agcggatcac caccatgctc tcgtcggccg acggaatgtc gcgggcgatg aaggggttcg
     1441 aggtatcgac gatcggcacg cgctggaaat tgacgtgggt gcggccgaac tgcgagcaga
     1501 tgtggttcac atagtcgggc atccggcgca ggatggtgtc gaccaccgct tcctgcgagt
     1561 agccgcgcat cttctggtcg cggtgcagtt tctggatcca ctccaggttg atgatcggca
     1621 cgacgccgac gcagagatcc acctgggcgg cgatgtcgat gccgtcgcca ctccaggcgc
     1681 cgtgcaggcc ttcgtagaac atcaggtcgg tagcggccgg gatgtcctgc cagggcgtga
     1741 aggtgccggg ctcctgctgc cagggcgcgg cctcggcggc gtcgtgcagg tacttgcgca
     1801 ccttgccgct accgtttttg ccgtagctca cgaagaggtc ctgcagttct tcgagcaggt
     1861 tggcttccgg gccgaagtgg ctgaaattga cgttgccctg ctccccctgc gccttcattt
     1921 cggcgcgcat cgacagccgg tcgtagcgat ggaaggaatc gccctcgacg atctgggcgt
     1981 tgatcttctc gcgccggaag atgtgctgga aactctgcat caccgtgctg gtgccggcgc
     2041 cggacgagcc ggtgatggcg atgatcggat gtttgacgga catggcggga actccgggaa
     2101 tgaggaagac ggtgggctag tcgcggaaca gcgaacgttc ggcgaacagc ggcgaggcgt
     2161 agggacggtc ggtgccggcg tcgaactcct gg
//
LOCUS       DAOSDX010000501         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17279_length_2189_cov_2.990628, whole genome shotgun sequence.
ACCESSION   DAOSDX010000501 DAOSDX010000000
VERSION     DAOSDX010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17279_length_2189_cov_2.990628"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLN02_10725"
     CDS             <1..834
                     /locus_tag="PLN02_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOI52841.1"
                     /translation="AVRGEGGLLKLPDGTRFMPEHDERCELAPRDIVARAIDFEMKKR
                     GLDCVYLDISHKGEDFIREHFPNIHARCLELGIDITREPIPVVPAAHYTCGGIVCDLN
                     GRTDVEGLYVAGEASCTGLHGANRLASNSLLECLVFSEAAVNDMLAQAPSELPVLPQW
                     DESRVTDADEEVVISHNWDELRRFMWDYVGIVRTTKRLQRAEHRVELLKQEIHEFYSH
                     FRVSHDLIELRNLVLTAELIIRCAMHRKESRGLHYSRDYPETAARAHNTVLTPSRWEN
                     H"
     gene            complement(806..1249)
                     /locus_tag="PLN02_10730"
     CDS             complement(806..1249)
                     /locus_tag="PLN02_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52842.1"
                     /translation="MQLPIIIGLHRSSLQRRIFLLVTLAVLLAILLLPVSLVWRSCCL
                     LLLLPILSRGWGRLNALPCRLRLERDGSVSLAQAGGDEFRQAYCLPGATVHPALCVFR
                     LREAGGGQTHVVLVTGDSTSADEFRRLRVFLRWRAVFSGSPSAME"
     gene            complement(1276..>2189)
                     /gene="fabF"
                     /locus_tag="PLN02_10735"
     CDS             complement(1276..>2189)
                     /gene="fabF"
                     /locus_tag="PLN02_10735"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOI52843.1"
                     /translation="GIGGLPLIEATKDEYNAAGVRKVSPFFVPGSIINMISGNLSIEF
                     GFKGPNLSIVTACTTGTHSIGEAARIIEYGDADVMVAGGAESTVSPLGLGGFCAARAL
                     STRNDDPATASRPWDKDRDGFVLGEGAGVLVLEEYEHAKARGAKIYAEVAGYGMSADA
                     YHITAPNMDGPRRSMVNALKNAGVSPADVQYVNAHGTSTPLGDKNECDAIKAAFGDAA
                     KSIVVNSTKSMTGHLLGGAGGIESLFTVLAIHHQVSPPTINIFNQDPECDLDFCANAA
                     RPMQIDIALKNNFGFGGTNGSLVFKRV"
BASE COUNT          441 a          720 c          667 g          361 t
ORIGIN      
        1 gcggtacgcg gcgaaggcgg cttgctcaag ctgccggacg gtacgcgctt catgccggag
       61 cacgacgagc gctgcgaact ggcaccacgc gacatcgtcg cccgcgccat cgatttcgaa
      121 atgaagaagc gcggcctcga ctgcgtctac ctcgatattt cgcacaaggg cgaagacttc
      181 atccgcgagc acttcccgaa cattcacgcg cgctgcctgg aactcggcat cgacatcacg
      241 cgcgaaccga tcccggtcgt cccggcggct cattacacct gcggcggcat tgtctgcgac
      301 ctcaacggtc gcaccgacgt cgaaggcctg tacgttgccg gtgaagcttc ctgcaccggc
      361 ctgcacggcg ccaatcgcct ggcctcgaac tcgttgctcg aatgcctcgt cttttcggag
      421 gccgcggtca acgacatgct ggcgcaagcg cccagcgaac tgccggtgct gccgcaatgg
      481 gacgagagtc gggtgaccga cgccgacgag gaagtcgtca tttcgcacaa ttgggacgag
      541 ctacgccgtt tcatgtggga ctacgtcggc atcgtccgta ccaccaagcg ccttcagcga
      601 gccgagcacc gggtcgaact gctgaagcag gagattcacg agttctactc gcacttccgc
      661 gtcagtcacg acctgatcga actgcgcaac ctggtcctga cggccgaact gatcatccgc
      721 tgcgccatgc accgcaagga aagccgcggc ctccactatt cccgggatta tccggaaacg
      781 gcggcgcgcg cgcacaacac ggtactcact ccatcgcgct gggagaacca ctgaagactg
      841 cgcgccagcg caggaagacc cgcagccggc gaaattcgtc ggccgacgta ctgtcgccgg
      901 tcaccagcac gacatgcgtt tgcccgccac ccgcttcgcg cagccggaac acgcacagcg
      961 ccggatgcac cgtcgcgcca ggcaaacaat aagcctgccg gaactcgtcg ccgccggcct
     1021 gagcaagcga aacgctgccg tcgcgctcga gacgcaaacg gcagggcagc gcgttcagcc
     1081 tgccccagcc ccttgacaag attggcaaga gcagcagcag gcagcaggaa cgccacacca
     1141 gcgacacagg caagagcagg atggccagca gcaccgccag cgtgacgagc agaaaaatgc
     1201 ggcgctgcaa tgaagaacgg tgcagtccga tgatgatcgg caactgcacc gttacacgct
     1261 ttcgcgacta gcgcctcaga cccgcttgaa caccagcgag ccgttggtac cgccgaaacc
     1321 gaagttgttt ttcagcgcga tgtcgatctg catcggccgc gccgcattgg cgcagaaatc
     1381 cagatcgcat tccggatcct gattgaagat gttgatggtc ggtggcgaca cctgatgatg
     1441 gatggccagc acggtgaaca gggattcgat accccccgcc ccgccgagca gatgaccagt
     1501 catcgacttg gtcgaattga cgacgatcga ttttgccgca tcgccgaaag ccgccttgat
     1561 cgcatcacac tcgttcttgt cgccgagcgg cgtcgaggtg ccatgggcgt tgacgtactg
     1621 aacgtcggcc ggcgacacgc cggcgttctt cagcgcattg accatcgaac ggcgcggacc
     1681 gtccatgttc ggcgcggtga tgtgataagc gtcagcactc atgccgtagc cagccacttc
     1741 ggcgtagatc ttcgcaccgc gggccttggc gtgctcgtat tcctcaagca ccagtacccc
     1801 ggcgccctcg cccagcacga aaccgtcgcg atccttgtcc cacggacggc tggcggtagc
     1861 cgggtcgtcg ttgcgggtgg acagcgcacg ggcggcgcag aagccgccca ggccgagcgg
     1921 cgagacggtc gattcagcac cgccggcaac catcacgtcg gcatcgccgt actcgatgat
     1981 gcgcgcggct tcgccgatcg aatgggtgcc ggtggtgcag gcggtaacga tcgacaggtt
     2041 cggccccttg aagccgaatt cgatcgacag gttgccggag atcatgttga tgatcgaacc
     2101 gggcacgaag aagggcgata ccttgcgcac gccagcggcg ttgtattcat ccttggtggc
     2161 ttcaatcagc ggcaggccgc cgatgccgg
//
LOCUS       DAOSDX010000502         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17335_length_2185_cov_2.989671, whole genome shotgun sequence.
ACCESSION   DAOSDX010000502 DAOSDX010000000
VERSION     DAOSDX010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17335_length_2185_cov_2.989671"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLN02_10740"
     CDS             <1..855
                     /locus_tag="PLN02_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI52844.1"
                     /translation="DAGGYSREFADWYLLPMAAAIWSCPTGQMRAMPLGPFVRFCNNH
                     GLLQIFDRPAWRTVSGGARTYVRKLAEQLDDVRVACPVSAVTREAGGLRVTHARGSDH
                     YDQVVMACHSDQSLAILGYTASDAQREVLAAIRYQPNRAILHTDLALLPRQRSLWSAW
                     NYFAGEGAPGEQPVGVSYLINKLQPLPFSTPVVVTLNPVSEPAPDKILAEFDYAHPVF
                     DAAAIAAQERLPEVQGENGIWLAGAWGSYGFHEDGLKSALRVANALGVQAPWQGSVAE
                     LLAETETA"
     gene            863..1633
                     /locus_tag="PLN02_10745"
     CDS             863..1633
                     /locus_tag="PLN02_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HOI52845.1"
                     /translation="MRGTPELFLGHVMHRRLRPAVNAFVYPVFYLQLPVRRLGECDCG
                     IFSVDRRNILSLRSKDHGPRDGSPLLPWITGVLREHGLPDDGEIMLQTFPRVFGYVFN
                     PVSFWYCRDRAGALIAVLAEVNNTFGGHHSYLLHDHGRPLADGAELQAAKIFHVSPFC
                     EVEGGYRFRFRLERETQVARIDYADADGDLLLSSISGRPQGWRAGSLLRNFLRMPLLT
                     AGVIFRIHWQALKLWLKGVPFVGANPGNKGPLLQETSK"
     gene            1630..>2185
                     /locus_tag="PLN02_10750"
     CDS             1630..>2185
                     /locus_tag="PLN02_10750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI52846.1"
                     /translation="MNDTLTLRGGRRPARDTRTLFELLAGLRGGLLEIRLPDGSNTLF
                     GDGDSGVSLQVHDEAMFSRVLARGDIGLAEAYLDGHWDSPDLSGVLALLAGNREALRQ
                     AVYGSWGALLLARLRHLLNRNSRSGSRRNILAHYDLGNDFYRLWLDRTMSYSAAIYRD
                     ADDGSLESAQQAKYRRILDRLQAQP"
BASE COUNT          330 a          789 c          703 g          363 t
ORIGIN      
        1 gatgctggcg gctattcgcg cgaattcgcc gactggtacc tgctgccgat ggcggccgcg
       61 atctggtcct gcccgaccgg acagatgcgc gccatgccgc ttggcccctt cgtccgtttc
      121 tgcaacaacc acggcctgct gcagattttc gaccggccgg cctggcgcac cgtcagcggc
      181 ggcgcccgga cctatgtgcg caaacttgcc gagcagctcg acgacgtccg tgtcgcctgc
      241 ccggtcagcg ccgtcacccg cgaagccggt ggcctgcggg tgacgcatgc gcgcggtagc
      301 gaccattacg accaggtagt gatggcctgc cacagcgacc agtcgctggc gattctcggc
      361 tacaccgcca gcgacgccca gcgcgaggtg ctggcggcga tccgctacca gccgaaccgg
      421 gcgatcctgc ataccgacct ggcgctgctg ccgcgccagc gttcgctgtg gtcggcgtgg
      481 aactactttg ccggcgaagg cgcgccgggc gagcagccgg tcggtgtttc ctacctgatc
      541 aacaagctgc agccgctgcc gttctcgacg ccggtggtag tgaccctcaa cccggtcagc
      601 gagccggcgc cggacaagat tctggccgag ttcgactatg cccatccggt gttcgacgcc
      661 gccgccatcg ccgcccagga aaggctgccc gaggtccagg gcgagaacgg catctggctg
      721 gccggcgcct ggggcagcta cggcttccac gaggacggcc tcaagtcggc cttgcgcgtc
      781 gccaatgcgc ttggcgtcca ggccccctgg cagggcagcg tcgccgaact gctggccgaa
      841 accgagacgg cctgagctca tcatgcgcgg cacgcccgaa ctcttccttg gccacgtcat
      901 gcatcgccgt ctgcggccgg cggtgaacgc cttcgtctat ccggtgttct acctccagct
      961 accggtgcgc cgattgggcg agtgcgactg cggcatcttc tcggtcgacc gccgcaacat
     1021 cctctcgctg cgcagcaagg accatggtcc gcgcgacggt tcgccgctgc tgccgtggat
     1081 caccggcgtg ttgcgcgagc acggcctgcc cgacgacggc gagatcatgc tgcagacctt
     1141 tccgcgcgtt ttcggatacg tcttcaaccc ggtcagcttc tggtactgcc gcgaccgcgc
     1201 cggcgcgctg atcgctgtcc tcgccgaagt gaacaacacc ttcggcggtc atcacagcta
     1261 cctgctgcac gaccacggcc ggccgctggc cgacggtgcc gaactgcagg ccgccaagat
     1321 cttccacgtc tcgccgttct gcgaggttga aggcggttat cgcttccgct tccgcctgga
     1381 gcgggagacc caggtggccc gcatcgacta cgccgacgct gacggcgacc tgctgctttc
     1441 ctcgatttcc ggccgcccgc agggctggcg ggcgggcagc ctgctgcgca acttcctgcg
     1501 catgccgctg ctcacggccg gcgtcatttt ccgcattcac tggcaggcgc tcaaattgtg
     1561 gctgaagggt gtgcccttcg tcggcgccaa cccgggcaac aagggcccgc tacttcagga
     1621 gaccagcaaa tgaacgacac cctgaccctg cgcggcggcc gccggccggc gcgcgacacc
     1681 cggacactgt tcgaactgct cgccggtctg cgcggcggcc tgctcgagat ccgcctgccg
     1741 gacggcagca ataccctgtt cggcgacggc gacagcggcg tcagtctgca ggtgcacgac
     1801 gaagcgatgt tcagccgggt actcgcccgc ggcgacatcg gcctggccga agcctacctc
     1861 gatggccact gggacagccc cgacctgagc ggcgtgctgg cattgctggc cggcaaccgc
     1921 gaggcgctgc gccaggccgt gtacggctcc tggggtgcct tgctgctggc gcgccttcgc
     1981 cacctgctca accgcaacag ccgcagcggc agccggcgca acatcctcgc ccattacgac
     2041 ctgggcaacg acttctaccg cctgtggctg gaccgcacga tgagctattc ggcggcgatc
     2101 tatcgcgacg ccgacgacgg tagcctggaa agtgcccagc aggccaagta ccggcgcatc
     2161 ctcgaccgcc tgcaggcgca accgg
//
LOCUS       DAOSDX010000503         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17349_length_2184_cov_3.162518, whole genome shotgun sequence.
ACCESSION   DAOSDX010000503 DAOSDX010000000
VERSION     DAOSDX010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17349_length_2184_cov_3.162518"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /gene="mraW"
                     /locus_tag="PLN02_10755"
     CDS             complement(<1..295)
                     /gene="mraW"
                     /locus_tag="PLN02_10755"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HOI52847.1"
                     /translation="MSSGGAHVTVLLEEAVNSLAIKKDGVYVDATFGRGGHSRRILEA
                     LGGNGHLVALDRDPQAIQAGSTIADPRFRLVHRAFGELAEAAAEAGVAAVDGVL"
     gene            complement(292..738)
                     /gene="mraZ"
                     /locus_tag="PLN02_10760"
     CDS             complement(292..738)
                     /gene="mraZ"
                     /locus_tag="PLN02_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOI52848.1"
                     /translation="MFEGATALSLDAKGRLAIPARHRDALLAAGAGSLVLTAHPHRCL
                     LLYPAPAWQPIRDQILKASSLDPRAAAIKRVMVGNARTEEPDSAGRLLVAPELRTYAQ
                     LEKTVYLVGMGSHFEIWSEAGWQQQNDLAAEALTGDLPAGLGDLVL"
     gene            complement(1061..1792)
                     /locus_tag="PLN02_10765"
     CDS             complement(1061..1792)
                     /locus_tag="PLN02_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOI52849.1"
                     /translation="MLLKAWSTVLFLLISTACSANEAEIQKAIESRLGVKVESVTKAG
                     YFGLYEIYADGNLFYTDEKVTAILIGGDLIRSQHALNLVDLKTNRNITEERLRKLTAI
                     KFSDLPLDRAIKQVRGDGKRVLATFEDPNCGYCKRLAKDMQKLENVTVYTFLYPILSE
                     DSLKKSRQIWCSADRAKVWNDWIIDGKTPGGRDNCDTTAIKRNLEFGRRLNITGTPTI
                     IFADGERVPGAVPLNVLEQRLSQQK"
     gene            complement(1802..>2184)
                     /locus_tag="PLN02_10770"
     CDS             complement(1802..>2184)
                     /locus_tag="PLN02_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOI52850.1"
                     /translation="QAFPLRLMRVPEIVARRLALVGDAGHGIHPLSGHGINLGFQDAQ
                     ALADQLLASKAWEDIGDLRFLQGYQRARREEITLMQTTTDALRRLFAAPSPLLRPLRN
                     LGLNFTNRLPVAKSVLVRYALGGF"
BASE COUNT          445 a          695 c          634 g          410 t
ORIGIN      
        1 acaacacccc atccaccgcc gccacgccgg cctccgcggc cgcttcggcc agttcgccga
       61 aagcgcggtg caccagccgg aaacgcgggt cggcaatcgt cgagccggcc tggatcgcct
      121 gcgggtcacg atcgagcgcg accaggtggc cgttcccccc cagcgcttca agaatgcggc
      181 gactgtggcc accacgaccg aaggtcgcgt ccacgtacac gccgtctttc ttgattgcca
      241 gggaattcac cgcctcttcg agcagcaccg tgacatgggc gccgccggaa ctcacaggac
      301 aaggtccccc agtcccgccg gcagatcgcc ggtcagcgcc tcggccgcca gatcgttctg
      361 ttgctgccag ccggcttcgc tccagatttc gaaatgactg cccatgccaa ccaggtacac
      421 cgtcttttcc agttgcgcgt aggtgcgcaa ctcgggtgcg accagcaggc gcccggcgct
      481 gtcgggctcc tcggtccgcg cattgccgac cattacccgc ttgattgcgg ccgcccgtgg
      541 atcaaggctg gaagccttga ggatctggtc gcggatcggc tgccaggccg gtgccggata
      601 aagcagcagg caacgatgcg ggtgcgcggt caggaccagc gacccggcgc cggccgcgag
      661 cagggcgtca cgatgccttg ccggaatggc aagccgccct ttcgcatcca gactgagcgc
      721 cgtagccccc tcgaacatcc ccgtttaccc acttttcaac acgttttccc actttagagc
      781 atgcactgat gggcgtcaat agaaaaacgg ggaatttttt cttacgcaac aatgacttac
      841 cgggattttt gaagacaaaa tgctgggcaa aaaagactta aaaatcaatg cacgaaaaac
      901 gttagttaaa gttaaacatt aggagtttgc ccggcaagcc caagccatca cgatgacatt
      961 tcctgtgcag caaaagcggc atgaaaaaac ggcacatcgc tccccatcag ggaaccatgt
     1021 gccgcctgtg gcgcccgtcg ccgagggcgc gtaaatgcct ttacttctgc tggctcaaac
     1081 gctgctcgag cacgttgagc ggcaccgcgc cgggcaccct ttcgccgtcg gcgaagatga
     1141 tggtcggcgt gccggtgata tttagacggc gaccgaactc caggttcctc ttgatcgccg
     1201 tggtatcgca gttatcccga ccgcccggcg tcttgccgtc aatgatccag tcgttccaga
     1261 ccttggcgcg gtcagccgag caccagatct gacgcgattt cttcagcgag tcctcggaca
     1321 ggatgggata gaggaaggta tagacggtga cgttttccag cttctgcatg tccttggcca
     1381 gacgcttgca atagccgcaa ttgggatctt cgaaggtcgc cagcacccgc ttgccgtcgc
     1441 cgcgcacctg cttgatcgcg cgatcgagtg gcaggtcgct gaacttgatc gccgtcagct
     1501 tgcgcagacg ctcctcggta atgttgcggt tggtcttcag gtcaaccagg ttgagcgcat
     1561 gctgggaacg gatcaggtcg ccgccgatca ggatcgccgt gactttctcg tcggtgtaga
     1621 aaagattgcc gtcggcgtaa atttcgtaca gcccgaaata accggccttg gtcacgcttt
     1681 cgaccttgac cccgagccgg gactcgatgg ctttctggat ttcggcttca ttcgccgaac
     1741 aggccgtcga gatcagcaaa aacaatacgg tggaccaggc cttcagcaac atcaaactct
     1801 cctagaagcc cccaagggcg taacgaacga ggacactctt tgcgaccggc aaacggttcg
     1861 taaagttcag ccccaggttg cgcaagggcc gcaacagcgg cgatggcgcg gcaaaaagac
     1921 gacgcagcgc atcggtggtc gtctgcatca aggtgatttc ctcacgccgc gcccgctgat
     1981 agccctgcag gaagcgcaga tcaccgatgt cttcccaggc cttgctggcc agcaactgat
     2041 cggcgagcgc ctgcgcatcc tggaagccca ggttgatacc gtgccccgac agcggatgga
     2101 tgccgtgacc ggcatcgcca accagcgcca ggcgccgggc gactatttcc ggtacccgca
     2161 tcaggcgcag cgggaaagcc tgcg
//
LOCUS       DAOSDX010000504         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17378_length_2181_cov_4.143932, whole genome shotgun sequence.
ACCESSION   DAOSDX010000504 DAOSDX010000000
VERSION     DAOSDX010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17378_length_2181_cov_4.143932"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLN02_10775"
     CDS             complement(<1..187)
                     /locus_tag="PLN02_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI52851.1"
                     /translation="MNSPTEIPAVTRQSERLGELIEERIVTGVYPPGTRLDEQELANT
                     FGVSRTLVREALIQLSSA"
     gene            377..1402
                     /gene="dctP"
                     /locus_tag="PLN02_10780"
     CDS             377..1402
                     /gene="dctP"
                     /locus_tag="PLN02_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOI52852.1"
                     /translation="MISKTKRYLSLAIAAATLSFALPAAHAQAPRVIKISHQFPAATT
                     EDGDFRDRLVRRFAAEVEKRSKGSLKFEIYPGSSLMKTNSQIGALRKSALDMSLVPLA
                     YGGGEIPQVNITLMPTLVSSYEQGMRWKNAPVGKELDRILADKNIKIVTWIWQAGGIA
                     STKKTVVVPDDAKGLKFRGGSKEMDQMLKGAGAAVTGMPSSEIYSAMQSGVLDAALTS
                     STSLISFRLQEFTKFATSARNRSFWFMFEPLLISKGLYDSLTPEQQKIVSEVGASLEQ
                     FGVEESKKDDARMAEVYAKAGAKVADMDDKAFAAWRAIAEQTSFKDFAENIKGGRELL
                     DMALSVK"
     gene            1455..2039
                     /locus_tag="PLN02_10785"
     CDS             1455..2039
                     /locus_tag="PLN02_10785"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOI52853.1"
                     /translation="MSPGVFISRAVKAFNIATGYLSAFLIVAATGILVFEVAVRYFFA
                     WPTDWEIELCVMLLIASSFLAAAHTQLNRGHVTIEILDEVTPHSWTQWRMALSDLLSF
                     LFCAFVAWHCWHLFYEAWDEGRMSDTSWAPKMWPVFATMAVGMTSLALQIFVQFIEDS
                     LPEAMRSHQPPPQHNAAIQVAIERIQRDAEGERK"
BASE COUNT          420 a          678 c          648 g          435 t
ORIGIN      
        1 cggccgaaga tagctgaata agcgcttcgc gcaccagcgt tcgcgagact ccgaaggtat
       61 tggcaagctc ctgctcatcg agccgcgtcc ccggcggata aaccccggtg acgatgcgtt
      121 cctcgatcag ttccccgagg cgttcggact ggcgtgtcac ggcaggaatt tcggtgggac
      181 tgttcacgat agtgctccaa cgattggtag ttattaaatt atacgaaatt agttgaccag
      241 tattcaatct ctgatattgt gtatacaaga cggtggcttt tgaacacaaa tagtgcggta
      301 ttcctgaatc gaggataaac cagccggtca gcgaggggtc accgttccta tgtcatcaac
      361 ccaggaggag acatccatga tcagcaagac caagcgctac cttagcctgg cgattgccgc
      421 cgccaccctg agctttgccc tgcctgccgc ccatgcccag gcgccgcgcg ttatcaagat
      481 ttcacaccag ttcccggcgg caacgaccga agacggggat ttccgtgacc gcctggtccg
      541 tcgcttcgcc gccgaggtcg aaaagcggag caaaggcagc ctgaagttcg agatctatcc
      601 gggcagctcg ctgatgaaga ccaacagcca gatcggcgcc ctgcgcaaga gcgcgctgga
      661 catgagtctg gtgcccctgg cctacggcgg tggcgaaatt ccgcaggtaa acatcaccct
      721 gatgccgacc ctggtcagca gctacgaaca gggcatgcgc tggaagaatg cgccggtcgg
      781 caaggagctc gaccgcatcc tggccgacaa gaacatcaag atcgtcacct ggatctggca
      841 ggccggcggc atcgcctcga ccaagaagac cgtggtcgtg ccggacgacg ccaaaggcct
      901 gaagttccgc ggcggcagca aggaaatgga tcagatgctg aagggcgccg gtgccgcggt
      961 gaccggcatg ccgtcgtcgg aaatctactc ggcgatgcag tcgggcgtgc tcgatgcggc
     1021 gctgacctcg agcacctcgc tgatcagttt ccgcctccag gagttcacca agttcgcgac
     1081 cagtgcgcgt aacaggagct tctggttcat gttcgaacca ctgctcattt ccaagggcct
     1141 gtacgactcg ctgacgccgg aacagcagaa gatcgtcagc gaggttggcg ccagcctgga
     1201 gcagttcggc gtcgaagaat cgaagaagga cgatgcgcgc atggccgagg tctacgccaa
     1261 ggccggcgcc aaggtcgccg acatggacga caaggctttc gcggcctggc gcgccattgc
     1321 cgaacagacc tcgttcaagg attttgccga gaacatcaag ggcggccgcg agctgctcga
     1381 catggcgctg tcggtcaagt gatccccggg gccgggcagg gcccggctcc tcctccttct
     1441 tcttgaggtg aatcatgagc cccggagttt tcatcagccg cgcggtcaag gccttcaaca
     1501 tcgccacggg ttacctgtcg gcctttctga tcgtcgccgc caccggcatt ctcgtgttcg
     1561 aggttgccgt ccgttatttc tttgcctggc cgaccgactg ggaaatcgag ctgtgcgtca
     1621 tgctgctcat cgcttccagc ttcctggccg ccgcccacac ccagttgaat cgcggccacg
     1681 tgaccatcga gattctcgac gaggtcacgc cgcatagctg gacgcagtgg cggatggccc
     1741 tttccgacct gctgtccttt ctgttctgcg ccttcgtcgc ctggcattgc tggcatctct
     1801 tctacgaggc ctgggacgag ggacgcatga gcgatacctc gtgggcgccg aagatgtggc
     1861 cggttttcgc caccatggcg gtcggcatga cctccctggc gctgcagatc ttcgtccagt
     1921 tcatcgagga ttccctgccg gaagcgatgc gttcgcatca gccgccgccc cagcacaacg
     1981 ccgccatcca ggtggccatc gaacgcatcc agcgcgatgc ggaaggagag cgcaaatgag
     2041 cgtcggcacc ctgggcctgc tctatgcagc agccaccttc atcttcctgt tttccggcat
     2101 gccgattgcg ttcgccgtcg gttcggtcgc cctgatcttc atgtacttct tcatgccggc
     2161 ggcgcagttg tcgatcatcg c
//
LOCUS       DAOSDX010000505         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17425_length_2177_cov_3.494816, whole genome shotgun sequence.
ACCESSION   DAOSDX010000505 DAOSDX010000000
VERSION     DAOSDX010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17425_length_2177_cov_3.494816"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLN02_10790"
     CDS             complement(<1..531)
                     /locus_tag="PLN02_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52854.1"
                     /translation="MKSPLKTWLPLALALLANLAQAADCPRIISQSPYISRALAWLGQ
                     EDCIVGVSRYDRLPLAHTGGVKDPDAAAMADLEPDVVIYSEWTAEATVESATPPGARA
                     VRVGGFRGMAGVEAMLREVGQAAGVADTERRVAQFASDWRQAAAQVDGRQRRVLILSA
                     CGEAPYSFGRGSTLFEV"
     gene            complement(641..>2177)
                     /locus_tag="PLN02_10795"
     CDS             complement(641..>2177)
                     /locus_tag="PLN02_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOI52855.1"
                     /translation="NDTAVAVNQGGKRKGALCAYLETWHLDIEEFLELRKNTGDERRR
                     THDMNTANWIPDLFMQRVIAGGEWTLFSPVDTPDLHEKSGRAFAEAYEAYEAKAAAGQ
                     LKLHKKIPAVQLWRKMLTMLFETGHPWITFKDACNLRSPQQHVGVVHSSNLCTEITLN
                     TSDSETAVCNLGSVNLLAHLKDGQLDQEKLARTVSTAMRMLDNVVDINFYATPKARNA
                     NLRHRPVGMGIMGFADCLYQLGLPYASPQAVEFADRSMEAVCYQAYWASTELAQERGR
                     YSSFTGSLWERGILPHESIALLEAGRGEAVELNRDSRLDWDALKQRIARHGMRNSNCV
                     AIAPTATISNIVGVSASIEPAYQNLYVKSNLSGEFTVVNEALVRDLKALDLWDEVMVA
                     DLKYFDGSLARIDRVPDALKARYATAFEIDPVWLIEAAARRQKWIDQAQSLNLYLALP
                     SGKKLDETYKLAWKRGLKTTYYLRTLGASQAEKAGGRDEAPVSEEAPKFCSIDNPECE
                     ACQ"
BASE COUNT          335 a          683 c          752 g          407 t
ORIGIN      
        1 cacctcgaac agcgtgctgc cgcgcccgaa ggaatacggc gcctcgccgc aggcggaaag
       61 gatcagcacc cggcgctggc ggccatcgac ctgcgccgcg gcctgccgcc agtcgctggc
      121 aaactgcgca acgcggcgct cggtgtcggc caccccggcc gcctgcccga cctcgcgcaa
      181 catggcctcg acccccgcca tgccacgaaa gccgccgacc cgcacggccc gcgcaccggg
      241 cggcgtggcc gactcgacgg ttgcctcggc ggtccattcg gaatagatga cgacatcggg
      301 ctcgagatcg gccatcgccg cggcgtccgg gtccttgacg ccgccggtat gagcgagcgg
      361 cagccggtcg taacggctga cgccgacgat gcaatcttcc tgccccagcc aggccagcgc
      421 ccggctgatg tagggcgact ggctgatgat gcgcggacaa tcggccgcct gggcgagatt
      481 ggcgagcagc gccagggcga ggggaagcca ggttttgagg ggggatttca ttggcaggcc
      541 tcgcactcgg ggttgtggct gcggccgctc gccttcgctc gcttaacccg gcgcgccggg
      601 ttagcctgcg ccgcaatccg tcgaataagc ttggctggct tcattggcac gcctcgcact
      661 ccggattgtc gatcgaacag aatttcggtg cttcttcgct cactggcgcc tcgtcccgcc
      721 cgccggcctt ttccgcctgg ctggcgccga gggtgcgcag gtagtaggtg gttttcaggc
      781 cgcgcttcca ggccagtttg taggtttcat cgagcttctt gcccgacggc agcgccaggt
      841 agaggttgag cgattgcgcc tggtcgatcc atttctggcg ccgcgccgcg gcttcgatca
      901 gccagaccgg gtcgatctcg aaggcggtgg cgtagcgggc tttcagcgcg tcgggcacgc
      961 ggtcgatgcg ggcgagcgag ccgtcgaagt acttgaggtc ggcgaccatc acctcgtccc
     1021 acaggtcgag cgccttgagg tcgcggacca gcgcttcgtt gaccacggtg aattcgccgg
     1081 agaggttgga tttcacgtag aggttctggt aggccggctc gatgctggcc gagacgccga
     1141 cgatgttgga gatggtcgcg gtcggcgcga tggcgacgca gttggagttg cgcatgccgt
     1201 ggcgggcgat gcgctgcttg agcgcgtccc agtcgaggcg gctgtcgcga ttgagttcga
     1261 ccgcttcgcc gcgcccggct tcgagcagcg caatgctctc atgcggcagg atgccgcgct
     1321 cccacaggct accggtgaag ctggagtagc ggccgcgttc ctgcgccagt tcggtcgatg
     1381 cccagtaagc ctggtagcag acggcttcca tgctgcggtc ggcgaattcg acggcttgcg
     1441 gcgaggcata cggcagccca agctggtaca ggcaatcggc aaaacccatg atgcccatgc
     1501 cgaccgggcg gtggcgcagg ttggcgttgc gcgccttggg cgtggcgtag aaattgatgt
     1561 cgacgacgtt gtcgagcatg cgcatggcgg tcgacacggt gcgcgcgagc ttttcctggt
     1621 caagctggcc gtccttcagg tgggcgagca gattgaccga gccgaggttg cacaccgccg
     1681 tttcggaatc cgaagtgttc agcgtgattt cggtacacag gttgctggaa tggacgacgc
     1741 cgacgtgctg ctgcggcgag cgcaggttgc aggcgtcctt gaaggtgatc cacgggtgcc
     1801 cggtctcgaa cagcatggtc agcatcttgc gccatagctg cacggcggga atcttcttgt
     1861 gcagcttcaa ctgcccggcg gcggccttgg cttcgtaggc ttcatacgcc tcagcaaagg
     1921 cgcggccgga tttttcgtgc aggtccggcg tatcaaccgg cgagaacaag gtccactcgc
     1981 caccggcaat cacgcgctgc atgaacaggt cgggaatcca gttggcggtg ttcatgtcgt
     2041 gcgtgcggcg gcgctcgtcg ccggtgttct tcctgagttc gaggaactcc tcgatgtcca
     2101 ggtgccaggt ttccaaatag gcgcagaggg cgcccttgcg cttgccgccc tgattcacgg
     2161 caaccgccgt gtcattg
//
LOCUS       DAOSDX010000506         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17448_length_2175_cov_3.583491, whole genome shotgun sequence.
ACCESSION   DAOSDX010000506 DAOSDX010000000
VERSION     DAOSDX010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17448_length_2175_cov_3.583491"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLN02_10800"
     CDS             <1..309
                     /locus_tag="PLN02_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOI52856.1"
                     /translation="LAGLILALSPLPLGADTAGMLLMILMLAVRGLIQLTVYILIGAL
                     ILQAVLSWVNPYSPVAPTVNQLVRPILAPIRRFVPTISGIDLSPLVAILLLQVVLMFV
                     "
     gene            309..599
                     /locus_tag="PLN02_10805"
     CDS             309..599
                     /locus_tag="PLN02_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOI52857.1"
                     /translation="MDDWYRVAADGKITLTLHIQPGAKKSECAGRHGDALKIRLAAPP
                     VDGKANAALTEFVAEHLGLPRAAVRLKSGQTSRRKILEITGSSPAAVARLAA"
     gene            complement(628..891)
                     /locus_tag="PLN02_10810"
     CDS             complement(628..891)
                     /locus_tag="PLN02_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOI52858.1"
                     /translation="MKRVTTLLFGMLAGIGAALAAVNLNTASVAELDAVKGIGPGKAK
                     AIVQYREKNGPFKSVDDLSQVKGFGKKSVDKLRAELTVGPAKK"
     gene            complement(1032..>2175)
                     /locus_tag="PLN02_10815"
     CDS             complement(1032..>2175)
                     /locus_tag="PLN02_10815"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOI52859.1"
                     /translation="GETVVDAAGRRLLPGMIDDQVHFREPGLEYKADIATESAAAVAG
                     GVTSVVVPPDADPPLDEPELADRLVHRAKEIRKARVFPLGALTLGLQGERLSELAGLK
                     KAGCVAFSQAKVPVVDTEALLRALEYAASFDFPVFLHPQDYWLSRNGIAHDGEVSARL
                     GLAGIPVAAETIAIATIVQLVRDTGCRVHLTRLSSAAGVALVKAAIDEGLPLSLDIGV
                     HHLLLTENDIGFFNAHARFLPPLRTRDDRAALSAAAAAGLAAICSDHTPVGADDKLVP
                     FGEAKPGATGLEVLLPLTLKWAEQEGIALPAALARITCAPAEILGLPGGTLTLGAAAD
                     LCIFDPAANWTLTPEALKSRGKNSPWLGYEMNGKVRHTLVDGRPVF"
BASE COUNT          342 a          731 c          742 g          360 t
ORIGIN      
        1 ctggccggcc tgatcctcgc gctgtcgccg ctgccgctcg gcgccgacac tgccggcatg
       61 ctgctgatga tcctgatgct ggcggtacgc ggcctgatcc agctcaccgt ctacatcctg
      121 atcggcgccc tgatcctgca ggcggtgttg tcctgggtca atccgtattc gcccgtggcg
      181 ccgacggtca accagctggt ccggccgatc ctggcgccga tccgtcgctt cgtgccaacg
      241 atttccggca tcgacctgtc gccgctggtc gctatcctgc tgctgcaggt cgtcctgatg
      301 ttcgtttgat ggacgactgg taccgggtcg ccgccgacgg caagatcacg ctgaccctgc
      361 acatccagcc cggcgcaaaa aagagcgaat gcgccggccg tcacggcgac gcgctgaaaa
      421 tccggctggc ggcgccgccg gtcgatggca aggcgaatgc cgcgctgacc gaattcgtgg
      481 ccgagcacct cgggttgccg cgggcggcgg tgcgcctgaa gagcggccag acctcgcggc
      541 gcaagattct ggagatcacc ggcagcagcc cggcagccgt cgcccgcctc gccgcctgaa
      601 ccggccggcg acggccaccc gaccggccta ttttttggcc ggccccaccg tcagttcggc
      661 gcgcagcttg tccacgctct tcttgccgaa acccttgacc tggctcaggt catcgaccga
      721 cttgaaggga ccgtttttct cccggtactg gacgatggcc ttggccttgc ccgggccgat
      781 gcccttgacc gcgtcgagtt cggcgacgct cgccgtgttc aggttgacgg cggcgagcgc
      841 ggcgccgata ccggccagca ttccgaacag caatgtggtg acacgcttca tctgaatttc
      901 ctccggaata acgatgcatc aggattattc gcgccggccc atccggcacg cggatttatt
      961 ctagatgaat atgacaatgt cgtcgtcgac aaaataatgc ggccacgcgg gccgcatcgg
     1021 aaaaacggaa ttcagaaaac cgggcgtccg tcgaccagcg tgtggcgaac cttgccgttc
     1081 atttcgtagc ccagccaggg cgaattcttg ccgcggctct tcagggcttc cggggtcagc
     1141 gtccagtttg ccgccgggtc gaagatgcag agatcggccg ccgccccgag tgtcagcgtg
     1201 ccgccgggca ggccaaggat ttccgccggt gcacaagtga tgcgggcgag cgcggcgggc
     1261 agggcgatgc cttcctgctc ggcccacttc agggtcagcg gcagcagaac ctcaagcccg
     1321 gtggcaccgg gcttcgcctc gccgaaaggc accagcttgt cgtcggcacc gaccggcgta
     1381 tggtcggagc agatcgccgc cagaccggcg gcggccgccg ccgacagcgc ggcgcggtcg
     1441 tcccgggtgc gcagcggcgg caggaagcgg gcatgcgcgt tgaagaagcc gatgtcgttc
     1501 tcggtcagca gcaggtgatg gacaccgatg tctaaactga gcggcaggcc ttcgtcgatc
     1561 gccgccttga ccagcgccac gccggccgcc gacgacagcc gcgtcaggtg gacgcggcag
     1621 ccggtgtcgc gcaccagttg gacgatggtg gcgatggcga tggtctcggc ggcgaccggg
     1681 atgccggcca ggccgaggcg ggcggaaact tcgccgtcgt gggcgatgcc gttgcgcgac
     1741 agccagtagt cctgcggatg aaggaagacc ggaaagtcga aactggccgc gtattcgagc
     1801 gcgcgcagca gggcctcggt atcgaccacc ggcaccttgg cctgcgagaa ggcgacgcaa
     1861 ccggccttct tcagaccggc cagttcggac aggcgctcac cctgcaggcc cagggtcagc
     1921 gcgccgagcg ggaagacgcg ggccttgcgg atttccttgg cgcgatgcac caggcggtcg
     1981 gcgagttccg gctcgtcgag cggcgggtcg gcgtccggcg gcacgacgac gctggtcacg
     2041 ccgccggcga ccgccgccgc cgactcggtg gcgatgtcgg ccttgtattc gaggcccggt
     2101 tcgcggaagt gcacctggtc gtcgatcatg cccggcagca gccggcggcc ggcggcgtcg
     2161 accacggtct cgccg
//
LOCUS       DAOSDX010000507         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17464_length_2174_cov_3.216140, whole genome shotgun sequence.
ACCESSION   DAOSDX010000507 DAOSDX010000000
VERSION     DAOSDX010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17464_length_2174_cov_3.216140"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PLN02_10820"
     CDS             <1..1173
                     /locus_tag="PLN02_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52860.1"
                     /translation="LTAKEAAEAANRAKTSFLANMSHELRTPMNGILGMIALAQKQVG
                     DPRPHEQLDKARRSAERLLAILNDILDLSRIEADRLHLEQAPFRLSSVFDNIDILFAQ
                     RAAEKGLRLRCEMPPALAKVSLLGDALRLGQILINLVGNAIKFSAQGDVVLRVSRRDA
                     GDGPAELRFEVEDSGIGISDADQQRIFAAFEQADGSMTRKYGGSGLGLAICRQLAQLM
                     GGDIGVVSTPGQGSVFWFVVRLSEVAAGEPVPSAASNALENEAALRAGFEGRRILLAD
                     DEPISREVGEILLAGAGLQVDTAEDGEQALALARQQTYDLILMDMQMPRCNGIDATRA
                     IRADSLNQRTPILAMTANVFNSDRDACLAAGMNDHLAKPLTPEALFAAVHRWLRAG"
     gene            complement(1230..1670)
                     /locus_tag="PLN02_10825"
     CDS             complement(1230..1670)
                     /locus_tag="PLN02_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52861.1"
                     /translation="MEPSDLAYATVAKQSFFRPFIRHIDMPYRPSSEHRKIPSVIVHL
                     TAAIHRVYGSRAQDQARPIGAFYPQHVKKDKWEHLGGIWYRAYNEQEILCRNLNAEVV
                     TAFVQSIRTGENARLANGRVITWEQIPSPEASLLPPPFPQSNPA"
     gene            complement(1671..>2174)
                     /locus_tag="PLN02_10830"
     CDS             complement(1671..>2174)
                     /locus_tag="PLN02_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI52862.1"
                     /translation="AADRADLVLGAPGDPPARGGISSRLLCRTVMRLVVAAGHPLASW
                     QGALPSAELMRHRAIVIADTSRELSARSIGLIEGQDALRVPDMRAKAQAQLAGLGVGH
                     LPAWLADPEIAAGRLVELQLADPRPPLQSQIAWRNRQIGKALRWFLDELAKPVTLAML
                     SEGLGTS"
BASE COUNT          365 a          723 c          725 g          361 t
ORIGIN      
        1 ctcaccgcca aggaagcggc ggaggccgcc aaccgggcga agaccagctt cctcgccaac
       61 atgagccacg aactgcggac gccgatgaat ggcatcctcg ggatgatcgc actcgcccag
      121 aaacaggtcg gcgacccgcg accgcacgag caactggaca aggcgcggcg ctccgccgaa
      181 cgcctgctcg ccatcctcaa cgacatcctc gacctgtcgc gcatcgaggc cgaccgcctg
      241 cacctcgaac aggcgccctt ccgcctgtcc agcgtgttcg acaacatcga catcctgttc
      301 gcccagcgcg ccgccgaaaa aggcctgcgc ctgcgttgcg agatgccgcc ggcgctggcc
      361 aaggtgagct tgctcggtga cgcgctgcgc ctcgggcaga tcctgatcaa cctggtcggc
      421 aacgcgatca agttcagcgc gcaaggcgac gtcgtgctac gcgtcagccg ccgcgatgcc
      481 ggcgacggcc cggccgaact gcgtttcgag gtcgaagaca gcggcatcgg catcagcgac
      541 gccgaccagc aacgcatctt cgccgccttc gaacaggccg acggttcgat gacccgcaag
      601 tacggcggca gcggcctcgg cctcgccatc tgccggcaac tggcgcaatt gatgggcggc
      661 gacatcggcg ttgtcagcac gcccggccag ggcagcgtct tctggttcgt cgtccgcctg
      721 tccgaagtgg cggctggcga gcctgtcccg tcagccgcgt cgaacgccct ggaaaacgag
      781 gccgcgctgc gcgccggatt cgaggggcgc cgcatcctgc tcgccgacga cgaaccgatc
      841 agccgcgaag tcggcgaaat cctcctcgcc ggcgccggcc tccaggtcga caccgccgaa
      901 gacggcgaac aggcgctggc gctcgcccgc cagcaaacct atgacctgat cctgatggac
      961 atgcagatgc ccaggtgcaa cggcatcgac gctacccgcg ccatccgcgc cgactctctg
     1021 aaccagcgca cgccgatcct ggcgatgacc gccaacgtct tcaacagcga ccgcgacgcc
     1081 tgcctcgccg ccggcatgaa cgatcacctg gccaagccgc tgaccccgga ggcgctgttc
     1141 gcggcggtgc atcgctggtt gcgcgcgggc taaggcgttg tggcgggcgc gtcgtccgtc
     1201 gcccaagcgg cagctttcgt tttcttcgct caggccggat tgctctgggg gaatggtgga
     1261 ggaaggagag atgcttctgg cgaggggatt tgctcccatg tgatgactcg gccattcgca
     1321 aggcgcgcgt tttctcccgt gcgtatgctc tggacaaagg ccgtgacgac ttcggcgttc
     1381 aggtttcgac agagtatttc ttgttcgttg tacgcgcgat accaaatgcc gcccaagtgc
     1441 tcccatttgt ctttttttac gtgctgcgga tagaaggcac ctatgggacg ggcttggtct
     1501 tgagcgcgac ttccatatac acgatgaatg gcagcggtca ggtgaacaat gacgctcgga
     1561 atcttgcggt gttcggagct tggtcggtaa ggcatgtcga tatggcgaat gaaagggcgg
     1621 aaaaagcttt gcttcgccac ggtagcatat gcaagatcag aaggctccat tcagctcgtt
     1681 cccagcccct cgctcaacat ggccagtgtc accggtttgg ccagttcgtc gaggaaccag
     1741 cgcagcgcct tgccgatctg ccggttgcgc caggcgattt gcgattgcag cggcggtcgc
     1801 gggtcggcga gctgcagttc gacgaggcgg ccggcggcga tttccgggtc ggccagccag
     1861 gccggcaggt ggccgacgcc gaggccggcc agttgggcct gggccttggc gcgcatgtcg
     1921 gggacgcgca gcgcgtcctg gccttcgatc aggccgatgc tgcgggcgct cagttcgcgc
     1981 gaggtgtcgg cgatgacgat ggcgcggtgg cgcatcagtt cggcgctggg cagcgctccc
     2041 tgccaactgg ccagcgggtg gccggcggcg accaccaggc gcatcaccgt ccggcacagc
     2101 aggcggctgc tgatgccgcc gcgcgccggc gggtcgccgg gggcgccgag cacgaggtcg
     2161 gcgcggtcgg cagc
//
LOCUS       DAOSDX010000508         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17508_length_2169_cov_4.167455, whole genome shotgun sequence.
ACCESSION   DAOSDX010000508 DAOSDX010000000
VERSION     DAOSDX010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_2169_cov_4.167455"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLN02_10835"
     CDS             <1..924
                     /locus_tag="PLN02_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOI52863.1"
                     /translation="GRLYAHEAEARLQMHPDDMAQRGLADGDLVRLSSRRGDSVLPVA
                     ASGELAPGGVYLAMHWGSANLSHPGANLHTLQELDPVSGQPALKHAAVQITPVSLPWQ
                     LAIVRACPGLDEVAAGERLLAWRRRLAPLLADQPYASLTLGGRESPVLILRLARAQAL
                     AQAEIERLADLLELPAERCRVLSDPARRIEKRARFEAVGTVRRLAGILFAGETAALPW
                     LLEHMTGGDAIGGLDPWVFLPSATPPAGGEGRGRIVCNCLNVSENEIHGGIAAGQRSL
                     EQLKATLKCGTACGSCVPEVRRMLAASEVAA"
     gene            921..1397
                     /locus_tag="PLN02_10840"
     CDS             921..1397
                     /locus_tag="PLN02_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52864.1"
                     /translation="MSAMPQTGWNAAAAEHGLVTFVHADARGVDGLGEAAVHALIDAS
                     VVLAGDEIDDSLLRLVPAHARIVQLGRPGGRPAAPRSFINRLMLIAATEGERVVHLLT
                     GSDGQQQLREAMPALRAAGVAIRIIELPTLADEVSGVARVDTLRAGRDAAGQQVLG"
     gene            1741..>2169
                     /locus_tag="PLN02_10845"
     CDS             1741..>2169
                     /locus_tag="PLN02_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI52865.1"
                     /translation="MSKENRSKPQFSVGRKMVRAALMTAAIAAQFAVVQAHAAGKIEK
                     ADLKFGFIKLTDMVPLAVAYEKGYFEDEGLYVTLEAQANWKVLLDRVISGELDGAHML
                     AGQPIGATIGYGTKADVITAFSMDLNGNAITVSNAVMEKLK"
BASE COUNT          371 a          709 c          722 g          367 t
ORIGIN      
        1 ggccgcctct acgcccacga agcggaagcg cgcctgcaga tgcatccgga cgacatggca
       61 cagcgcggtt tggccgacgg cgatctggtc cgtcttagtt cgcgccgcgg cgacagcgtg
      121 ctgccggtgg cggccagcgg cgaactggcg ccgggcggcg tctatctcgc catgcactgg
      181 ggctcggcca acctgtcgca ccccggcgcc aacctgcaca ccctgcagga actcgatccg
      241 gtcagcggcc agccggcgct caagcatgcc gcggtgcaaa tcacgccggt cagcctgccc
      301 tggcaactgg ccatcgtccg cgcctgtccc ggactggacg aggtggcggc cggcgaacgc
      361 ctgctcgcct ggcggcgccg gctcgccccg ttgctcgccg atcagcccta cgccagcctg
      421 accctgggcg ggcgcgaatc gccggtgctg atcctgcgtc tggcgcgcgc tcaggcgctg
      481 gcgcaagcgg aaatcgagcg gctggccgat ctgctcgagc tgccggccga gcgttgccgt
      541 gtcctcagcg acccggcccg gcgtatcgaa aagcgcgctc gcttcgaggc tgtcgggacg
      601 gtgcgccgcc tggccggcat cctgttcgcc ggcgaaaccg ccgccctgcc ctggttgctc
      661 gaacacatga ccggcggcga cgcgatcggt gggctcgatc cctgggtctt cctgccctcg
      721 gcgacaccgc cggccggtgg cgaagggcgc ggccgcatcg tctgcaattg cctcaatgtc
      781 tcggaaaacg aaattcacgg cggcattgcc gccggccagc gcagcctgga acaactcaag
      841 gcgacgctca agtgcggcac cgcctgcggt tcctgcgtgc cggaagtgcg ccgcatgctg
      901 gcggccagcg aggtggcggc atgagcgcca tgccgcaaac cgggtggaac gccgccgcgg
      961 cggaacacgg actggtcact ttcgtccacg ccgacgcccg cggtgtcgac ggcctgggcg
     1021 aggcggctgt gcatgccctg atcgacgcca gcgtcgtgct ggccggcgac gagatcgacg
     1081 actccctgct ccgcctggtg ccggcgcacg cccgcatcgt ccagctcggg cgtccgggcg
     1141 gtcgtccggc ggcaccgcgc agtttcatca atcgcctgat gctgatcgcc gcgacggagg
     1201 gcgagcgggt ggttcatttg ttgaccggga gcgacgggca gcaacaactg cgcgaagcca
     1261 tgcccgcctt gcgcgcggcg ggcgttgcca tccgcatcat cgagttgccg acgctggccg
     1321 atgaagtgtc cggcgtggcg cgtgtcgata cgcttcgcgc cggacgggat gcggccgggc
     1381 agcaggtgct tgggtaagcc agacaaggcg caccgatcag gggcaaacgg cagggcaaaa
     1441 gtcatttaat ggtgcgctgc aataagcgat ttgaccggtg tgtttgtttt aagttgttga
     1501 tttaacgatg ttgatttttg tggcacggtg gttgctaagt taaacgcaac tgcgcattgc
     1561 cggcaattcc gaagtcgcag cgctccaacg gtggagtcgc tgcaagcagg aacggcaatc
     1621 gtgagcatgc ttggcctaag gtagggtcgt catgctggga caacggcgtc ctcgagagcg
     1681 aaaccaggtt tcgctacgag ggcgcctttt tttttgggat ttttttcagg agtctacaaa
     1741 atgagcaagg aaaaccgcag caagccgcaa ttctccgtcg gccggaaaat ggtccgtgcc
     1801 gccctgatga ccgcagccat cgcggcgcaa ttcgccgtcg ttcaggctca cgccgccggc
     1861 aagatcgaaa aggcggacct gaaattcggc ttcatcaagc tgaccgacat ggttccgctg
     1921 gccgtcgctt acgaaaaggg ctatttcgag gacgaaggcc tctacgtcac gctggaagcc
     1981 caggccaact ggaaagtcct gctcgaccgc gtgatttccg gcgaactcga cggcgcccac
     2041 atgctggccg gtcagccgat cggcgccacc atcggctacg gcaccaaggc cgacgtcatc
     2101 accgctttca gcatggacct caacggcaac gcgatcaccg tgtcgaacgc cgtcatggag
     2161 aagctgaag
//
LOCUS       DAOSDX010000509         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17541_length_2166_cov_3.352440, whole genome shotgun sequence.
ACCESSION   DAOSDX010000509 DAOSDX010000000
VERSION     DAOSDX010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17541_length_2166_cov_3.352440"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..567)
                     /gene="cowN"
                     /locus_tag="PLN02_10850"
     CDS             complement(250..567)
                     /gene="cowN"
                     /locus_tag="PLN02_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(2)-fixation sustaining protein CowN"
                     /protein_id="HOI52866.1"
                     /translation="MEPNREAAADCPCRKGSDRYVSFDGIDCDGNARRLMEFILRNVE
                     AQGSDNPFWNYFLAKRTPKSGPAPDDLFLVHAHINQIRDFFEACGDEEAMALLVQVEE
                     ECC"
     gene            complement(646..1308)
                     /locus_tag="PLN02_10855"
     CDS             complement(646..1308)
                     /locus_tag="PLN02_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOI52867.1"
                     /translation="MESVDTPSRPLHGAVSDIPPGLLDGFVAHEVARGTLFATPGDKR
                     DQVLIVGQGRLRVYLAGENRELSLSYLEPGDIYTTHTPTYLEAVTPCTLWLMDTRAFA
                     RKLASDPSVTPAMMRVLGRLLANAVSLIEDLAFRDVPARLARFISGLIERRGRPLDGG
                     VWLVPLELGTEDIASLLGTTRQTVSSLLNQWERDGILRRQGRRVLFIHALDTLRRLGA
                     EA"
     gene            complement(1400..>2166)
                     /locus_tag="PLN02_10860"
     CDS             complement(1400..>2166)
                     /locus_tag="PLN02_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOI52868.1"
                     /translation="SRLGGQVLSWTTPDGRERLYLSERAVFDGSAAIRGGVPVCFPQF
                     AERGDLPKHGFVRTREWEVSARRSGDDYALVTLETADDEATRALWPHRFRLELTLMIE
                     ADRIDLELCVSNTGELPFEFTAALHTYLRVVQVEDVAIEGLYGHDYRDAANGNKVVRD
                     SGTELLIERETDRVYRHVRRPQMLRAGNLSLGIQQQGFPDTVVWNPWVEKCAALPDMA
                     DDDWRHMLCIEAAAADVVQELGAGDEWYGRQTLVAL"
BASE COUNT          381 a          716 c          713 g          356 t
ORIGIN      
        1 catcgcggca tgcatctgcg ccattgcgcc ggcatggtgg tcgtgcccgc cttcctgcat
       61 tgccactgcc tcgtcgacgc cttgcgccag cgcgcgcggc aaggtcgccg ggaacagcac
      121 ggaggcgacg acgatcagca ggaggcagcg cgccagcagg cggcggatgg atttcatgcg
      181 gcaactataa acgagtcacg gacgctctga aaccacccgc tggaaaaagt tcaacgcgcg
      241 gctggcgctt cagcagcact cttcctcgac ctggaccagc aaggccatcg cttcctcgtc
      301 gccacaggct tcgaagaaat cgcgaatctg gttgatgtgg gcatggacca ggaacaggtc
      361 atccggcgcc ggcccgctct tcggcgtgcg cttggccagg aagtagttcc agaagggatt
      421 gtcgctgccc tgcgcttcga cgttgcgcag gatgaattcc atcagccggc gggcgttgcc
      481 atcgcaatcg atcccgtcaa aactgacata gcggtcgctg cccttgcggc acggacaatc
      541 ggcggcggct tcccggttcg gctccatggt cttgctcctc aagcgataac gccaccatgg
      601 tagcgggcgt ccctcgcgcc gtccgtcggc tggccgacag ttcgctcagg cttcggcacc
      661 gagccggcgc aacgtgtcca gcgcgtggat gaagagcacg cgccgcccct ggcggcgcag
      721 gatgccatcg cgctcccact ggttgagcag cgacgatacc gtctgccggg tcgtgccgag
      781 caggctggcg atgtcttccg tgccgagttc gagcggtacc agccagacgc caccgtcgag
      841 cggccggccg cgccgttcga tcaagccgct gatgaagcgc gccaggcgcg ccggcacgtc
      901 gcggaacgcc agatcctcga tcagcgagac ggcattggcc agcagacggc cgagcacgcg
      961 catcatcgcc ggcgtcaccg aggggtcgct ggccagcttg cgggcgaagg cgcgagtgtc
     1021 catcagccac aacgtgcacg gcgtcaccgc ctccagatag gtcggcgtat gcgtcgtgta
     1081 gatatcgccg ggttcgagat agctcaggct gagttcccgg ttctcgccag ccagatagac
     1141 gcgcagccgg ccctgcccga cgatcagcac ctggtcgcgc ttgtcgcccg gcgtcgcgaa
     1201 cagggtgccg cgcgccactt cgtgcgcaac aaaaccgtcg agcagacccg gcggaatgtc
     1261 cgaaacggcg ccatgcagag gtcgtgaagg agtgtcgacg gattccatcc ccagcctcaa
     1321 gcaagacgag gaccaggaaa aaaccttttg cgatcagtcc cctgacgagg ccttcgactg
     1381 tcgcatgcgc gacaatccgc tacaaggcaa ccagggtctg gcgaccgtac cactcgtcgc
     1441 cagcgccaag ttcctgaacg acgtcggccg cggcggcttc gatgcacagc atgtggcgcc
     1501 agtcgtcgtc cgccatgtcg ggcagcgcgg cgcacttttc cacccagggg ttccagacca
     1561 cggtatccgg aaagccctgt tgctggatgc ccaggctgag gttgccggcg cgcagcattt
     1621 gcgggcggcg gacatggcga tagacgcggt cggtttcgcg ttcgatgagc aattcggtac
     1681 cgctgtcgcg gaccaccttg ttgccgttgg cggcatcccg gtagtcgtga ccgtaaagcc
     1741 cctcgatggc gacatcttcg acctggacga cgcgcaggta ggtgtgcagc gcggcggtga
     1801 attcaaaggg cagctcaccg gtgttgctga cgcagagttc gaggtcgatg cggtcggctt
     1861 cgatcatcag cgtcagctcg aggcggaagc gatgcggcca gagtgcccgc gtcgcctcgt
     1921 cgtcggcggt ttccagggtg accagcgcgt agtcgtcgcc actgcgccgg gcgctgactt
     1981 cccattcccg ggtacgcacg aagccgtgct tgggcaagtc gccgcgctcg gcgaactgcg
     2041 ggaagcagac cgggacgcca ccgcggatcg ccgcgctgcc gtcgaacacc gcccgctcgg
     2101 aaagatagag ccgttcgcgc ccgtcgggcg tggtccacga caggacctgg ccgccaagac
     2161 ggctga
//
LOCUS       DAOSDX010000510         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17551_length_2165_cov_4.404739, whole genome shotgun sequence.
ACCESSION   DAOSDX010000510 DAOSDX010000000
VERSION     DAOSDX010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17551_length_2165_cov_4.404739"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..>2165
                     /locus_tag="PLN02_10865"
     CDS             281..>2165
                     /locus_tag="PLN02_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002944786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOI52869.1"
                     /translation="MTDLSVAKKLVPYKPRNKVRFVTAASLFDGHDASINIMRRILQA
                     TGAEVIHLGHNRSVQEIVNAALQEDAQGICITSYQGGHVEFFKYMIDLLKANGGENIK
                     VFGGGGGVIVPAEIKELHAYGVTRIYAPEDGVHMGLQGMINEVVEQSDYDLAGAGIPK
                     ADKVLEGLRAGDRRLLARVITLLENGAAPTLKEPLLKAAAATTIPVLGITGTGGAGKS
                     SLTDELVRRFRLDQEDSVKIGLISIDPSRKRTGGALLGDRIRMNAIEHPNIFMRSLAT
                     RDTGSEISAALPEVIAACKLAGFDLVVVETSGIGQGDAAIVPFVDVSMYVMTPEFGAA
                     SQLEKIDMLDFADFVAINKFDRKGAEDALRDVRKQYQRNRELFTTPTENMPVFGTMAA
                     RFNDDGVTALYQALVPALSEKGLQLKSGKLPLVTVRQSSASRAIVPTARIRYLAEIAD
                     TVRGYHKHVDEQVKIARERQSLRIARNVFAACEKSVGDFDEMQAFKDSQQDGKAKKLL
                     DMWPQTVEAYSGDEYVVKIRDKEIRTQLTSESLSGTKIRKVILPKYTDDGEILRFLMK
                     ENVPGSFPYTAGVFAFKREGEDPTRMFAGEGDAFRTNRRFKRVSEGMPAHRLSTAFDS
                     VTLY"
BASE COUNT          415 a          728 c          668 g          354 t
ORIGIN      
        1 gatcgacgcc ggcggcgtgc agcagcggcg ccggatcgat gttgcggcgg gcaagcccgg
       61 ccagcatccc ggtgacaaat cccattgcaa cagtggtttg gcgtttggtt tcgcgttggc
      121 tcatggtgcg gcgcagcaat cgagctgcgc tccggcgcta gacggcgcca gcatatcacc
      181 ttggcggaat ttgcatgatt gtcgtcaggg ctggcaatgg cggggcgcgc aaagcgtcct
      241 aagatgagcg ccttcccgtc aatttcgcga ggccgatgtc atgaccgatc tttccgtcgc
      301 caagaaactc gttccctaca agcccaggaa caaggtccgt ttcgtcaccg ccgcctcgct
      361 gttcgacggc cacgatgcgt cgatcaacat catgcgccgc atcctgcagg cgaccggcgc
      421 cgaagtgatc catctcggcc ataaccgctc cgtgcaggag atcgtcaatg ccgcgctgca
      481 ggaagatgcc cagggcatct gcatcacctc ctaccagggc ggccacgtcg aattcttcaa
      541 gtacatgatc gacctcctga aggccaacgg cggcgagaac atcaaggtct tcggcggcgg
      601 tggcggtgtc atcgtgccgg ccgagatcaa ggaattgcat gcctacggcg tgacccgcat
      661 ctacgcgccg gaagacggcg tgcacatggg tctgcagggg atgatcaacg aagtcgtcga
      721 acagtccgac tacgacctcg ccggcgcagg catcccgaag gccgacaagg tgctcgaagg
      781 cctgcgcgcc ggcgatcgcc gcctgctcgc ccgggtcatc accctgctcg agaacggcgc
      841 cgcgccgacg ctcaaggagc cgctgctcaa ggccgccgcc gcgacgacca ttccggtgct
      901 cggcatcacc ggcaccggtg gcgccggcaa gtcgtcgctg accgacgaac tggtccgccg
      961 cttccgcctg gaccaggaag acagcgtcaa gatcggcctg atctcgatcg acccgtcgcg
     1021 caaacgtacc ggcggcgcgc tgctcggcga ccgtatccgg atgaacgcca tcgagcaccc
     1081 gaacatcttc atgcgctcgc tggccacccg cgacaccggc tcggaaatct ccgccgcgct
     1141 gccggaagtg atcgccgcct gcaagctcgc cggcttcgat ctggtcgtcg tcgaaacctc
     1201 cggcatcggt cagggcgacg ccgcgatcgt gcccttcgtc gatgtctcga tgtacgtgat
     1261 gacgccggag ttcggcgccg cttcgcagct ggagaagatc gacatgctcg acttcgccga
     1321 cttcgtggcg atcaacaagt tcgaccgcaa gggcgccgag gacgcgctgc gcgacgtgcg
     1381 caagcagtac cagcgcaacc gcgagttgtt cacgacgccg accgagaaca tgccggtgtt
     1441 cggcaccatg gccgcgcgct tcaacgacga cggcgtcacc gcgctttacc aggcgctggt
     1501 cccggcgttg agcgaaaagg gactgcaact gaagtccggc aaactgccgc tggtcaccgt
     1561 ccgccagtcg tcggccagcc gcgccatcgt gccgactgcc cgcatccgct acctggccga
     1621 gatcgctgac acggtgcgcg gttatcacaa gcacgtcgac gagcaggtga agatcgcccg
     1681 cgagcgccag tcgctgcgca tcgccaggaa cgtattcgcc gcttgcgaga agtcggtggg
     1741 tgacttcgac gaaatgcagg cgttcaagga cagccagcag gacggcaagg cgaagaagct
     1801 gctcgacatg tggccgcaga ccgtcgaggc ctacagcggc gacgaatacg tggtgaagat
     1861 ccgcgacaag gaaatccgca cccaactgac ttccgaatcg ctgtccggca ccaagatccg
     1921 caaggtgatc ctgcccaagt acaccgacga cggcgaaatc ctgcgcttcc tgatgaagga
     1981 gaacgtgccg ggttccttcc cctataccgc cggcgtcttc gccttcaagc gagagggtga
     2041 agacccgacc cgcatgttcg ctggcgaagg cgacgccttc cgtaccaacc gccgcttcaa
     2101 gcgcgtctcc gaaggcatgc cggcccaccg tctgtccacc gccttcgact cggtgacgct
     2161 gtacg
//
LOCUS       DAOSDX010000511         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17593_length_2161_cov_3.755461, whole genome shotgun sequence.
ACCESSION   DAOSDX010000511 DAOSDX010000000
VERSION     DAOSDX010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17593_length_2161_cov_3.755461"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PLN02_10870"
     CDS             <1..1158
                     /locus_tag="PLN02_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOI52870.1"
                     /translation="FEHNLGALIDRQRQNGQGHGTITVNGRTINLILRGNLPASPTAG
                     RVYNVDSGSRPAAARRAEAPCPQVLTLDTLNTGDARLQLAIDRARRLLGRDIPIMIQG
                     ESGAGKEMFAKAFHNSGPRADGPFVALNCASIPESLIESELFGYQGGAFTGARKEGAP
                     GKIQQAHGGTLFLDEIGDMPMNMQARLLRVLQERAVTPLGSTREIQVDISLVCATHRK
                     LREEVARGGFREDLYYRLNGMSVTLPPLRERSDLRQLVAKLAAGESCERGPVRFTEGA
                     LQVIERYAWPGNIRQLFNVIRVAIALLDDDETTVHESHLPEELFEAPLAPASGEAQWA
                     AAPLENPVGSLEEVGRQLAQRALDQANGNVSAAARQLGISRNTLYRKLGKL"
     gene            complement(1153..1533)
                     /locus_tag="PLN02_10875"
     CDS             complement(1153..1533)
                     /locus_tag="PLN02_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HOI52871.1"
                     /translation="MNTYLLLRHLHISCVVLSGAGFLLRLYWMASASPRLRQRLTRTL
                     PHVIDSVLLGSALSLAVWSGQYPFVDGWLTAKLGGLLAYIGLGTMALKRGRTRAARLG
                     YGALAVLAYAYIVGVALTRNPFWL"
     gene            complement(1530..>2161)
                     /locus_tag="PLN02_10880"
     CDS             complement(1530..>2161)
                     /locus_tag="PLN02_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52872.1"
                     /translation="RPAWGFPLFLFAVLLAWSAALAFDADWPARLGRLLLAAGGAAFA
                     VQTLRLQAKRRRARPDTTFRYWQFGLYGALLALAILAAGALVPALADDPRWTPAFGLV
                     LLLGAFVPLIVGMLAKIVPFLLWLDLQNHGLVGKALPPMNKLLPEAPMQRQFQVFVAA
                     AGLALLAVLLPDWLSRPAGVLLIAAAVWLAVNLLQASWRHGRQLQQAPA"
BASE COUNT          397 a          774 c          748 g          242 t
ORIGIN      
        1 ttcgagcaca atctgggcgc gctgatcgac cgccagcggc agaacggcca gggccacggc
       61 acgatcacgg tgaacggccg gacgatcaac ctgatcctgc gcggcaacct gccagcttcg
      121 cccaccgccg gtcgcgtcta caacgttgac agcggcagcc gtccggccgc cgcccgacgc
      181 gccgaagcgc cctgcccgca ggtgttgacg ctggacaccc tgaacaccgg cgacgcccgc
      241 ctgcaactgg cgatcgaccg cgcccggcgg ctgctcgggc gcgacatccc gatcatgatc
      301 cagggcgaat ccggcgccgg caaggagatg ttcgccaagg ccttccacaa cagcggcccg
      361 cgcgccgacg gccccttcgt cgccctcaac tgcgcgtcga tcccggaaag cctgatcgaa
      421 tccgaactct tcggctacca gggcggcgcc ttcaccggcg cgcgcaagga aggcgcgccg
      481 ggcaaaatcc agcaggcgca cggcggcacc ctcttcctcg acgaaatcgg cgacatgccg
      541 atgaacatgc aggcgcggct gctgcgcgtg ctgcaggaac gcgcggtgac gccgctcggc
      601 agcacgcgcg agatccaggt cgacatctcg ctggtctgcg ccacccaccg caagctgcgc
      661 gaggaagtcg cccgcggcgg cttccgcgag gatctctact accggctcaa cggcatgagc
      721 gtcaccctgc cgccgctgcg cgagcgcagc gacctgcgtc aactggtcgc caagctggcc
      781 gccggcgaaa gctgcgagcg cggcccggtg cgcttcaccg aaggcgcgct gcaggtgatc
      841 gagcgctacg cgtggccagg caacatccgc cagttgttca acgtgatccg cgtcgccatc
      901 gccctgctcg acgacgacga gacgacggtg cacgaatcgc atctgccgga agaactgttc
      961 gaggcgccgc tggcgcccgc cagcggcgaa gcacagtggg cggcggcacc gctggagaac
     1021 ccggtcggca gcctggagga agtcggccgc cagctcgccc agcgcgcgct cgaccaggcc
     1081 aacggcaacg tctcggcggc ggcgcgccag ctcggcatca gccgcaacac gctctaccgc
     1141 aagctcggca agttatagcc agaacggatt gcgggtcagc gcgacgccga cgatgtaggc
     1201 gtaggcgagc accgccagcg cgccgtagcc caggcgggcg gcgcgcgtcc ggccgcgctt
     1261 caaggccatc gtgcccagtc cgatgtaggc gagcagaccg cccagcttgg cggtcagcca
     1321 gccatcgacg aaaggatact ggccgctcca caccgccagg ctcagcgcgc tgccaagcag
     1381 cacgctgtcg atgacatgcg gcagggtgcg ggtcaggcgc tggcgcaggc gcggcgacgc
     1441 gctcgccatc cagtacaggc ggagcaggaa gccggcaccg ctgaggacga cgcaggagat
     1501 atgcaggtgg cgcagcagca ggtaggtgtt catgccggcg cctgttgcaa ctgccggccg
     1561 tgccgccacg acgcctgcag caggttgacg gccagccaga ccgcagcggc aatcagcagc
     1621 acacccgccg ggcggctcag ccagtcgggc agcaacaccg ccagcagcgc cagtccggca
     1681 gcggcgacga agacctggaa ctggcgctgc atcggcgcct cgggcagcag cttgttcatc
     1741 ggcggcaggg ccttgccgac caggccgtgg ttctgcaggt cgagccagag caggaaggga
     1801 acgatcttgg ccagcatgcc gacgatcaac ggcacgaaag cgccgagcag caagaccagg
     1861 ccgaaggccg gcgtccaccg cggatcgtcg gcgagcgccg ggaccagcgc gcccgcagca
     1921 agtatggcga gcgccagcaa tgcgccgtag aggccgaatt gccagtaacg gaaggtggtg
     1981 tcgggccgcg cccgccgccg tttggcctgc aggcgcaggg tctgcaccgc gaaggcggcg
     2041 ccgccggcgg cgagcagcag gcggccgagc cgcgccggcc aatcggcgtc gaacgccagc
     2101 gcggcgctcc aggcaagcag cacagcgaac aggaagagcg ggaaacccca cgccgggcgc
     2161 g
//
LOCUS       DAOSDX010000512         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17698_length_2152_cov_2.960420, whole genome shotgun sequence.
ACCESSION   DAOSDX010000512 DAOSDX010000000
VERSION     DAOSDX010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17698_length_2152_cov_2.960420"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLN02_10885"
     CDS             <1..672
                     /locus_tag="PLN02_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOI52873.1"
                     /translation="LRFITRAGPEIGVASTKAFSTQLVALFLLTLVLAKVKGRLSSED
                     ESCHLEQLRHLPVAVGKVLTLEPEIAAWAARFANKKDALFLGRGRHYPIALEGALKLK
                     EISYIHAEAYPAGELKHGPLALVDAEMPVISVAPNDELLEKLKSNLQEVRARGGELYV
                     FADAGSEIAESDGVHILHLPEHYGQLSPVLHVIPLQLLSYHAALVKGTDVDKPRNLAK
                     SVTVE"
     gene            complement(738..>1862)
                     /locus_tag="PLN02_10890"
     CDS             complement(738..>1862)
                     /locus_tag="PLN02_10890"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOI52874.1"
                     /translation="KGEHKRYLYTAAALGRTARIIDAQAAADIDWITLSETHSGVPVL
                     ETSIKRDTFRKTLQQDASLGDLRRTVEQVNALPRREEEVFAKKDEVEFDWGFAPGYNQ
                     NLGGPDAFVLYQLTANLDAQLRLSENTWIDGLMSFNLYNNFDKFTYTAPSNLPRVRTY
                     LREYMISSDVIMPRLQLNHVKRMNSDWYAMAYGGFLESMYAGVGGEILYRPLNSNLAL
                     GFNLNWVKQRGFKQDFALRDYSVVTGHASAYFRNIVPTVAPGLNLTLSAGRYLAKDLG
                     VTFDVSKEFDNGVRMGAWATFTNVSAEQFGEGSFDKGFYISLPFDLMTTASTRRWANI
                     VLQPLTRDGGARLNRGPTLYEITEGRNVDFFHKNFSSILQ"
     assembly_gap    1863..1872
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1873..>2152)
                     /locus_tag="PLN02_10895"
     CDS             complement(<1873..>2152)
                     /locus_tag="PLN02_10895"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOI52875.1"
                     /translation="NNYKNETGGPTVKQDRPFNIGLTYRHSDYLAFNIGWERGNTLLF
                     GINFTGDFSPRRGVPRKVSDPAPVPVRPDNSRPAIANDWPEIVRQIEDN"
BASE COUNT          414 a          621 c          662 g          445 t
ORIGIN      
        1 ctgcgcttca tcacccgcgc cggcccggaa atcggcgtcg cctcgaccaa ggccttctcc
       61 acgcaactgg tcgcgctgtt cctgcttacc cttgtcctgg ccaaggtcaa aggccgcttg
      121 agcagcgagg atgaaagctg ccatctcgag caactgcgcc atctgccggt cgccgtcggc
      181 aaggtgctga cgttggaacc ggaaattgca gcctgggcgg cacgattcgc caacaagaag
      241 gatgcgctct tcctgggtcg tggccggcat tacccgatcg cactggaagg cgcactcaag
      301 ctcaaggaaa tctcctacat ccacgccgaa gcctatccgg ccggcgaact caagcatggc
      361 ccgctggccc tggtcgatgc cgagatgccg gtgatttccg ttgctcccaa cgacgaactg
      421 ctggaaaagc tgaaatccaa cctgcaggaa gtgcgcgccc gtggcggaga gctgtatgtc
      481 tttgccgatg ccggctcgga aatcgccgag agcgacggcg tgcatatcct gcatctgccg
      541 gagcactacg gacagctttc tcccgtcctg catgtcattc ccctgcaact gctttcctac
      601 catgccgcgc tggtcaaggg gacggatgtc gacaagccac ggaatctggc gaaatcggtc
      661 accgtcgaat aaggcaggac tcgaaaaaag aaaacccggt gcgataccgg gttttttgct
      721 tctggcctgt ggtttttcta ttgaaggatg cttgagaaat tcttgtggaa gaagtccaca
      781 ttgcgaccct cggtgatttc gtagagcgtc gggccgcgat tcagccgtgc gccgccatcg
      841 cgggtcagcg gttgcaggac gatattcgcc cagcgccgcg ttgaggccgt ggtcatcagg
      901 tcgaaaggca aggagatgta gaaacccttg tcgaaactgc cttcgccgaa ttgctccgcg
      961 gagacgttgg tgaaggtggc ccaggccccc atgcggacgc cgttgtcgaa ttccttggaa
     1021 acgtcgaaag tcacgcccaa gtccttggcc aggtagcgac cggcgctgag tgtcaggttc
     1081 agtccgggcg ccacggtcgg cacgatgttg cggaaatagg ccgaggcatg gccggtcacg
     1141 accgagtaat cacgcagggc aaagtcctgc ttgaagcccc gctgcttgac ccagttgaga
     1201 ttgaagccca gggccaggtt gctgttcagc ggacgataga ggatttcgcc accgacgccg
     1261 gcgtacatcg attccaggaa accaccgtag gccatggcgt accagtcgct gttcatgcgc
     1321 ttgacgtgat tcagttgcag gcgcggcatg atcacgtcgg aactgatcat gtactcccgc
     1381 agataggtcc ggacgcgggg caggttgctc ggggccgtgt acgtgaactt gtcgaagttg
     1441 ttgtacaggt tgaagctcat caagccatcg atccaggtgt tttccgagag acgcaactgg
     1501 gcatcgaggt tggccgtcaa ttgatacagc acgaacgcat ccggcccgcc cagattctgg
     1561 ttgtagccgg gggcgaaacc ccagtcgaac tcgacctcat cctttttcgc gaacacttcc
     1621 tcctcgcgcc tcggcagggc attgacctgc tcgacggtcc gtctcagatc gcccagagag
     1681 gcgtcttgtt gcagggtctt gcggaaggtg tcgcgcttga tcgaggtttc gagtaccggt
     1741 acgccgctgt gcgtttccga aagcgtgatc cagtcgatat cggcggcggc ctgagcgtcg
     1801 atgatacggg ccgtgcggcc gagtgcggca gcggtgtaca agtagcgctt gtgttcgccc
     1861 ttnnnnnnnn nnattgtctt caatttgccg aacgatctcc ggccagtcgt tggcgatcgc
     1921 cggacggctg ttgtccggac gaaccggcac cggcgccgga tcggaaacct tgcgcggcac
     1981 gccgcggcgg ggactgaaat cgccggtgaa gttgatgccg aacaacagcg tgttgccacg
     2041 ttcccagccg atgttgaaag ccaggtaatc gctgtggcgg taggtcaggc cgatattgaa
     2101 gggtcggtcc tgcttgaccg tggggccgcc tgtttcattc ttgtaattgt tg
//
LOCUS       DAOSDX010000513         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17738_length_2148_cov_3.381271, whole genome shotgun sequence.
ACCESSION   DAOSDX010000513 DAOSDX010000000
VERSION     DAOSDX010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17738_length_2148_cov_3.381271"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..963)
                     /gene="argC"
                     /locus_tag="PLN02_10900"
     CDS             complement(13..963)
                     /gene="argC"
                     /locus_tag="PLN02_10900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOI52876.1"
                     /translation="MSYKVFVDGQEGTTGLQINEYLAQRADIEILKIDADKRKDLAER
                     KRLINASDVTFLCLPDAAAKESASLVDNPNTCIIDASTAHRVNPDWVFGLPELCPEQR
                     AKIRASKRIANPGCHASAFILALRPLVAAGLLTADTQIAANSITGYSGGGKSMIADYE
                     AAAASTTQLKAPRAYALALAHKHLPEMQAYTGLSVAPIFQPIVGPFYKGLAVTAYLHP
                     AQFTRAATPADVQKIIADYYAGERFINVLPVDLEATTDGGFFDVEACNGSNRVDIAVF
                     GNAERMLLIARLDNLGKGASGAAVQAMNVHLGIEESAGLL"
     gene            complement(1088..1648)
                     /gene="efp"
                     /locus_tag="PLN02_10905"
     CDS             complement(1088..1648)
                     /gene="efp"
                     /locus_tag="PLN02_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOI52877.1"
                     /translation="MKTAQELRSGNVIMVGSDPLVVQKTEYNKSGRNAAVVKMKLKNL
                     LSGNPSEAVYKADDKFEVVVLDKKEVTYSYFADPMYVFMDADYNQYEVEAENMTDALK
                     YLDDGMPCEVVFYDGKAISVELPTMLVREVEYTEPAVKGDTSGKVMKPARIKPTGFEL
                     PVPAFVEIGDKIEIDTRTDEYRSRTK"
     gene            complement(1685..>2148)
                     /gene="earP"
                     /locus_tag="PLN02_10910"
     CDS             complement(1685..>2148)
                     /gene="earP"
                     /locus_tag="PLN02_10910"
                     /inference="COORDINATES: protein motif:HMM:NF021598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOI52878.1"
                     /translation="QPLAAVRACLGGDGPWQLGLARIEPIPFLAQDDYDALLRRCAIN
                     FVRGEDSFVRAQWAGRPFVWQIYRQDDDAHLGKLDAFLDRYCADLAEPAARALRQLFL
                     AWNTGADCTAAWADFLGHYDAIAHHAAGWNAALRMQPDLATNLVKFCAAQV"
BASE COUNT          360 a          609 c          764 g          415 t
ORIGIN      
        1 tgctgaggac gatcaaagca ggccagcgct ttcctcaatg ccgagatgca cgttcatcgc
       61 ctgcacggcg gcgccggaag cgcccttgcc gaggttgtcg agacgggcga tcagcagcat
      121 gcgttcggcg ttgccgaaga cggcgatgtc gacgcggttg ctgccgttgc aggcttcgac
      181 gtcgaagaag ccgccatcgg tggtggcttc gagatcgacc ggcaggacat tgatgaagcg
      241 ctcgccggca tagtagtcgg caatgatctt ctgcacgtcg gccggcgttg cggcgcgggt
      301 gaattgcgcc gggtgcaggt aggcggtgac ggccaggccc ttgtagaacg ggccgacgat
      361 gggctggaag atcggtgcca cgctcaggcc agtgtaggcc tgcatttccg gcaggtgctt
      421 gtgcgccagg gcgagcgcgt aggcacgcgg tgccttcagt tgcgtagtgc tggcggctgc
      481 ggcttcgtag tcggcgatca tcgacttgcc gccgccggag taaccggtga tggaatttgc
      541 cgcaatctgc gtgtcggccg tgagcaagcc ggcggcaacc agcggacgca gcgccaggat
      601 gaaggcgctg gcgtggcagc cggggttggc gatgcgcttg ctggcgcgga tcttggcgcg
      661 ctgctccggg cacagctcgg gcaggccgaa gacccagtcc ggattgaccc ggtgcgcggt
      721 cgaggcgtcg atgatgcagg tgttcgggtt atccaccagg ctggccgact ccttggccgc
      781 cgcgtcgggc aggcagagga aagtcacgtc ggaggcgttg atcaggcgct tgcgctcggc
      841 cagatccttg cgcttgtcgg cgtcgatctt gaggatttcg atatcggcgc gctgcgcgag
      901 gtattcgttg atctgcaggc cggtggtgcc ttcctggccg tcgacaaaga ctttgtagct
      961 catggtgggt ccgcggttcg ggttggttga acgggaattc tggcagaaaa acatgtcgcg
     1021 atggcgacag gccggaaaag acaagggcag ccgaagctgc ccctggcggt tccggcgttg
     1081 gctggaatta cttggtacgg ctgcggtatt cgtcggtacg cgtatcgatt tcgatcttgt
     1141 cgccgatttc gacgaaggcc ggcaccggca gttcgaaacc ggtcggcttg atgcgggccg
     1201 gcttcatgac cttgcccgag gtgtcgccct tgacggccgg ctcggtgtat tcgacttcgc
     1261 gcaccagcat ggtcggcagt tcgaccgaga tcgccttgcc gtcgtagaag acgacttcgc
     1321 aaggcatgcc gtcgtcgagg tacttgagcg cgtcggtcat gttctcggct tcgacttcgt
     1381 actggttgta gtcggcgtcc atgaacacgt acatcgggtc ggcgaagtag gagtaggtga
     1441 cttccttctt gtcgagaacg acgacctcga acttgtcgtc ggccttgtag accgcttccg
     1501 acgggttgcc ggaaagcagg ttcttcagct tcatcttgac gacggcggcg ttgcggccgg
     1561 acttgttgta ttcggtcttc tggacgacga gcgggtcgct gccgaccatg atcacgttgc
     1621 cagagcggag ttcctgagcg gtcttcatgt gggatatccc aaagcggaaa aataaaacgc
     1681 gagattatac ctgcgcggcg cagaatttga cgagattcgt cgccagatcg ggctgcatgc
     1741 gcaaggccgc gttccagccc gcggcgtggt gggcgattgc gtcgtaatgg ccgagaaaat
     1801 cggcccaggc ggcggtgcaa tcggcccctg tgttccaggc caggaatagc tggcgcagcg
     1861 cgcgagccgc cggctcggcg agatcggcgc agtagcggtc gaggaaggcg tccagcttgc
     1921 ccaggtgggc gtcgtcgtcc tgccggtaga tctgccagac gaaaggccgg ccggcccact
     1981 gggcacggac gaaggaatcc tcaccgcgca cgaagttgat cgcgcagcgg cgcagcagcg
     2041 cgtcgtagtc gtcctgggca agaaagggga ttggctcgat gcgcgccagg ccgagctgcc
     2101 acggtccgtc gccgcccagg caggcgcgca ccgcagccag cggctggc
//
LOCUS       DAOSDX010000514         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17742_length_2148_cov_2.900621, whole genome shotgun sequence.
ACCESSION   DAOSDX010000514 DAOSDX010000000
VERSION     DAOSDX010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17742_length_2148_cov_2.900621"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..567)
                     /locus_tag="PLN02_10915"
     CDS             complement(82..567)
                     /locus_tag="PLN02_10915"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOI52879.1"
                     /translation="MHLPQHQLAMTVLMTPDMANFSGNVHGGTILKLLDQVAYACAAR
                     YASEYVVTLSVDQVMFRQAIHVGELVTFLASVNYTGNTSMEIGIKVLTEDIRAKVIRH
                     ANSCFFTMVAVDADGKPTPIPPLTPTTPEEIRRYQAAQVRRELRKEFEGRMQALTPKA
                     A"
     gene            complement(644..1138)
                     /locus_tag="PLN02_10920"
     CDS             complement(644..1138)
                     /locus_tag="PLN02_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HOI52880.1"
                     /translation="MANKKAKSPSIDIGISDADRKKIVEGLSGLLADSYTLYLMTHNF
                     HWNVKGPQFNSLHLMFMGQYTEQWNALDLIAERIRALGFPAPGTYKEFVKLASIKEVE
                     GVPSADDMVRHLVAAQEATARTARKLFPVVAEADDQPTADLLTQRLEVHEKTAWMLRS
                     LLED"
     gene            complement(1339..1632)
                     /locus_tag="PLN02_10925"
     CDS             complement(1339..1632)
                     /locus_tag="PLN02_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOI52881.1"
                     /translation="MRHTSCSLTITEKADADIRGDLETLARRWAPDGDPAYRRDTEGE
                     DDMASLARSVLTGVSLNIPCANGRLLLGRLQGIYLWEHRAQAHQREIVVSVLG"
     gene            1893..>2148
                     /locus_tag="PLN02_10930"
     CDS             1893..>2148
                     /locus_tag="PLN02_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOI52882.1"
                     /translation="MSRIALAPMEGLADPLMRDVLTAVGGYDWGICEFVRVTESVLPN
                     RTFLRTCPELKNGSRTPAGTPMRVQLLGSDPHFMAGNARRL"
BASE COUNT          349 a          601 c          749 g          449 t
ORIGIN      
        1 taggtttgag ttccatggct gcaatgtagt tgcgtctgta atttctgtca acgcagaaat
       61 aacctgcgtc agccggggcg ctcaggcggc cttcggcgtc agcgcctgca tccgtccctc
      121 gaattccttg cgcagttcgc gccgcacctg cgccgcctgg tagcggcgga tttcttccgg
      181 cgtggtcggc gtcagcggcg ggatcggcgt cggcttgccg tcggcgtcga cggcaaccat
      241 ggtgaagaaa caggagttgg cgtggcggat gaccttggcg cggatgtctt cggtcagcac
      301 cttgatgccg atttccatcg aggtgttgcc ggtgtaattg accgaggcga gaaaagtgac
      361 cagttcgccg acgtggatgg cctggcggaa catcacctgg tcgaccgaca gcgtgacgac
      421 gtattcgctg gcgtagcgcg cggcgcaggc gtaggcgacc tggtcgagca gcttgaggat
      481 ggtgccgccg tgaacgttgc cggagaagtt ggccatgtcc ggcgtcatca ggacggtcat
      541 cgcgagttgg tgctggggaa ggtgcatggc ggaagattca agtcggaaaa tgaaaaaggg
      601 caggatcgta atcctgcccc gcgtgcgtca cagccttggc acctcagtct tcgagcaggc
      661 tgcgcagcat ccaggcggtc ttttcatgga cttccaggcg ctgggtgagc aggtcggcgg
      721 tcggctgatc gtcggcttcg gcgaccaccg ggaacagctt gcgcgcggtg cgggcggtgg
      781 cttcctgggc ggcgaccagg tggcggacca tgtcgtcggc gctcggcacg ccttcgactt
      841 ccttgatcga ggcgagcttg acgaattcct tgtaggtgcc cggcgccggg aaacccaagg
      901 cgcggatgcg ctcggcgatc aggtcgaggg cgttccactg ctcggtgtat tggcccatga
      961 acatcaggtg caggctgttg aactgcgggc ctttgacgtt ccagtggaag ttgtgcgtca
     1021 tcaggtacag cgtgtagctg tcggcgagca ggccggacag gccttcgacg atcttcttgc
     1081 ggtcggcgtc ggaaatgccg atgtcgatgc tcggggattt ggctttcttg tttgccatgg
     1141 acggttctcc ttcgtgaaaa cagcgggttg ggcgcatcgc tgcgcgacct gaccgggagc
     1201 ggcgctggct tacctcggcg ggccgtttcc gggcgaatcc tgcgttggcg gcaggggctt
     1261 acgtcgttgg caaatatagc gcatatcagc ttcgccaggc caatcaatcg ggactatggg
     1321 agggttggcg cctgcctatc accccagcac gctgacgaca atctcgcgct ggtgggcctg
     1381 ggcgcggtgt tcccagaggt agatgccttg cagtctgccg agcagcaggc ggccgttggc
     1441 gcaggggatg ttgagggaaa cgccggtgag cacgctgcgg gccagcgagg ccatgtcgtc
     1501 ctcgccttcc gtatcgcgcc gataggccgg atcgccgtcg ggggcccagc gtctggcgag
     1561 ggtttccagg tcgccgcgaa tgtcggcgtc ggctttttcg gtgatggtca gcgagcagga
     1621 tgtgtgacgc acgaaaacat tgaccaggcc gctgggacgc ctgatgtggc gacgatatgc
     1681 gcaatttcgc tggtgatttc cgtcatcatg cgcggtagcg tttcaacaag aatgcttttc
     1741 tgatgtttca tcgccaggtc tcacgcggca ggtgtaattg caggaattgc agtcgggtcc
     1801 gtaaacggct gtctggcatt atcgcggctc gcacggtctt gcgcaatccg cgataatgcg
     1861 cgccatttca agtatttgcc gaaaggttgc ggatgtcacg aatcgctctt gccccgatgg
     1921 aaggcctcgc cgatccgctg atgcgcgacg tgctgacggc ggtgggcggc tatgactggg
     1981 gcatctgcga gttcgtccgg gtgaccgaga gcgttctgcc caaccggacc ttcctgcgca
     2041 cctgtccgga gctgaagaac ggcagccgga cgccggccgg gacgccgatg cgggtccagt
     2101 tgctcggttc cgacccgcat ttcatggccg gcaacgcgcg gcgcctgg
//
LOCUS       DAOSDX010000515         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17751_length_2147_cov_3.451243, whole genome shotgun sequence.
ACCESSION   DAOSDX010000515 DAOSDX010000000
VERSION     DAOSDX010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17751_length_2147_cov_3.451243"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PLN02_10935"
     CDS             complement(<1..1531)
                     /locus_tag="PLN02_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOI52883.1"
                     /translation="MTRLKTLPWLLASLALPLHAQQPAAPEVQQALRQVAQQAVQKSP
                     EVTIKWRNYKAAEEEVGVARGGYFPKLDLTAGAGRESLEVPGSAKDTYTRRGYALTLS
                     QMLFDGFATRSEVRKLGKAKLVRYYELLEASENVALEASKAYADVLRYRSLVALAEDN
                     YVRHKATYEQIKARTASGVGRRVDLDQAASRLALAEVNLTTETANLHDVMARYQRLVG
                     ELPAGTLTPQQLTGAALPASPKAALDVLYKRNPTLLAAVENIEAAQYDVEGRRAAYMP
                     RVDLRARTETTKNYQGVSGDRDQNVAEVVMSWNLFNGGSDTARERQYVERKHMALEMR
                     EKACRDTRQTLLIAYNDVQRLRDQKEDLTTQVALLEKTRDAYREQFNIGQRTLLDLLD
                     TENELLAAKRSLVNADTDLTLAYLRSQAGMGSLLEFMGLQKLETATPETGELADVDAA
                     TLCPPEPVALTSTPRADLDARAQDMLGKKPPVVTPPPASDADVQARIRAWAQAWSAKD
                     YT"
     gene            complement(1535..1858)
                     /locus_tag="PLN02_10940"
     CDS             complement(1535..1858)
                     /locus_tag="PLN02_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI52884.1"
                     /translation="MNTELICKNGQDIRELRNQLGLKQSTFWKRILVTQSGGSRYETG
                     RTIPKQVLLLIHLAYSPLKESEALLKQLHAGKPVPMLREISERPRVRRSHGGRAPSVV
                     QAGAC"
     gene            complement(1883..>2147)
                     /locus_tag="PLN02_10945"
     CDS             complement(1883..>2147)
                     /locus_tag="PLN02_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HOI52885.1"
                     /translation="AGGDVLDLRDLLQGEFHDGSDLGNLAQYLQIETASDHTLIRINP
                     LGDGSAPTQQLILDGVDLSAGGLHAGTAQILENLIQQGRLLVD"
BASE COUNT          275 a          694 c          754 g          424 t
ORIGIN      
        1 cggtgtagtc ctttgccgac caggcctgcg cccaggcgcg gatgcgcgcc tgcacgtcgg
       61 cgtcgctggc gggcggcggc gtcacgaccg gcggcttctt gcccagcatg tcctgcgccc
      121 gggcgtcgag atcggcgcgc ggcgtgctgg tcagcgccac cggctccggc gggcacagcg
      181 tggcggcgtc gacgtcggcc agttcgccag tttccggcgt cgccgtctcc agcttctgca
      241 ggcccatgaa ctcgagcagc gagcccatgc cggcctggct gcgcaggtag gccagcgtca
      301 ggtcggtatc ggcattgacc agcgagcgct tcgccgccag cagttcgttt tcggtatcga
      361 gcaggtcgag cagcgtgcgc tggccgatgt tgaactgttc gcggtaggcg tcgcgcgtct
      421 tctcgagcag tgccacctgc gtcgtcaggt cttccttctg gtcgcgcagg cgctgcacgt
      481 cgttgtaggc gatcaacagc gtctgccggg tgtcgcggca agccttctcg cgcatttcca
      541 gcgccatgtg cttgcgctcg acgtattgcc gttcgcgcgc cgtgtccgag ccgccgttga
      601 acaggttcca cgacatcacg acttcggcga cgttctggtc gcggtcgccg ctgacgccct
      661 ggtagttctt ggtcgtctcg gtgcgcgccc gcagatcgac gcgcggcatg taggcggcgc
      721 gccggccttc gacgtcgtat tgcgccgctt cgatgttctc gaccgctgcc agcagcgtcg
      781 ggttgcgctt gtagagcacg tcgagcgccg ccttcgggct cgccggcaac gccgcgccgg
      841 tcagttgctg cggcgtcagc gtgccggccg gcagttcgcc gaccagacgc tggtagcgcg
      901 ccatcacatc atgcaggttg gcggtctcgg tggtcaggtt gacctcggcc agcgccagcc
      961 ggctggccgc ctgatcgaga tcgacccggc ggccgacgcc ggaggcggtg cgtgccttga
     1021 tctgctcgta ggtcgccttg tgccgcacgt agttgtcttc cgccagcgcg accagcgagc
     1081 ggtagcgcag cacgtcggcg taagccttgc tggcttcgag ggcgacgttt tccgaggctt
     1141 cgagcagttc gtaatagcgc accagcttgg ccttgcccag cttgcgcact tcgctgcgcg
     1201 tcgcgaagcc gtcgaacagc atctggctca aggtcagcgc gtagccgcgg cgggtgtagg
     1261 tgtccttggc cgaacccggc acttccaggc tctcgcgacc ggcaccggcc gtcagatcga
     1321 gtttcgggaa gtagccgccg cgggcaacgc cgacttcttc ttcggcggcc ttgtagttgc
     1381 gccacttgat cgtcacttcc gggcttttct gcaccgcttg ttgcgccacc tggcgcagcg
     1441 cctgctggac ttccggagct gccggctgct gggcatggag cggcagggcg aggctggcga
     1501 gcagccaggg gagggtcttg agtcgggtca tcgctcagca ggctccggcc tggaccacgc
     1561 ttggcgcgcg gccgccatgg ctgcgccgga cgcgcggccg ttcgctgatc tcgcgcagca
     1621 tcggcaccgg cttgccggcg tgcaactgct tgagcagcgc ttccgattcc ttgagcgggc
     1681 tgtaggcgag atggatcagc agcagcacct gcttgggaat ggtgcgcccg gtttcgtagc
     1741 gcgagccgcc cgactgggtg accaggatgc gcttccagaa agtgctttgc ttgaggccaa
     1801 gctgattccg cagctcgcgg atatcctggc cattcttgca gatgagttcg gtgttcatgc
     1861 ggattctcct agtaccaagg gttcaatcga ccagcaggcg tccctgctgg atcaggtttt
     1921 cgaggatttg ggcggtgccg gcatggaggc cgccggccga cagatcgacg ccgtcgagga
     1981 tcagttgctg ggtcggcgcg ctgccgtcgc cgagcgggtt gatgcggatc agggtgtggt
     2041 cgctggcggt ctcgatctgc aggtactggg cgagattgcc gaggtcgctg ccgtcgtgga
     2101 attcgccctg cagcaggtcg cgcaggtcga gcacgtcgcc gccggcg
//
LOCUS       DAOSDX010000516         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17783_length_2144_cov_3.646242, whole genome shotgun sequence.
ACCESSION   DAOSDX010000516 DAOSDX010000000
VERSION     DAOSDX010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17783_length_2144_cov_3.646242"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLN02_10950"
     CDS             <1..782
                     /locus_tag="PLN02_10950"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOI52886.1"
                     /translation="EAARESLSARDLVDIVYGDPYLALKLLRRAEERRTRQLGHDTTT
                     PLAAVLQTGYDELQALVANSKVSLGLLPGCHACEQRAVLAAILARAWATRRVDVLPDE
                     VAMAALLAEIGELLLWHCAPEMTDKEVAIYQWNERYWALQQRTSEMDFTFRQLTMALA
                     QSWGLPTLIVALIKGVDSPRANLARLGHEAAKAVIGEATTPELLALLQEVGALLPAVD
                     LLAIAEPLPLADEPRAALQAAIAAVKAAASGEQPAQGGDQA"
     gene            complement(744..1814)
                     /gene="hisC"
                     /locus_tag="PLN02_10955"
     CDS             complement(744..1814)
                     /gene="hisC"
                     /locus_tag="PLN02_10955"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOI52887.1"
                     /translation="MSRFWSQVARDLTPYVPGEQPKIANLIKLNTNEHPFPPSPRVVE
                     AIRAELGDDGASLRLYPDPNADLLKQAIAAHHGVTTREVFVGNGSDEVLAHAFQALLK
                     HDAPLLFPDITYSFYPVYCGLYGVDHVAVPLAADFSIDPADYCGVANGGILIANPNAP
                     TGRLLPLAAIERIVAANPDSVVLVDEAYVDFGGTSAIPLTRQYDNLLVVHTLSKSRSL
                     AGLRVGFAVGHPDLIEALERVKNSFNSYPLDRLAIVGAVAAIEDEAYFRQCCAAVIAS
                     RESLVGQLAELGFDVLPSAANFIFARHPQRDAGELAQALRAESIIVRHFRLPRIDQFL
                     RITVGSDEQCSSLVATLRRLLA"
     gene            1892..>2144
                     /locus_tag="PLN02_10960"
     CDS             1892..>2144
                     /locus_tag="PLN02_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOI52888.1"
                     /translation="MRQAEVTRNTLETQITVKLNLDGSGQGKFATGVPFLDHMLDQIA
                     RHGLIDLDIDAKGDLHIDAHHTVEDIGITLGQALAKAWGD"
BASE COUNT          366 a          676 c          769 g          333 t
ORIGIN      
        1 gtgaggcggc gcgggaaagc ctctcggcgc gggatctggt cgatatcgtc tatggcgatc
       61 cctatctggc gctgaagctg ctgcgccggg ccgaggagcg gcgcacccgg caactggggc
      121 acgacacgac gacgccgctg gcggccgtcc tgcaaaccgg ttacgacgag ttgcaggcgc
      181 tggtcgccaa cagcaaggtc tcgctcggtc tcttgcccgg ttgccacgcc tgcgagcagc
      241 gcgcagtcct cgccgccatc ctggcgcggg cctgggcgac gcgccgggtg gacgtgctgc
      301 ccgacgaagt ggcgatggcg gcgctgctgg cggaaatcgg cgaactgctg ctgtggcatt
      361 gcgcgccgga aatgaccgac aaggaagttg ccatctacca gtggaacgag cgctactggg
      421 cgctccagca gcgtacttcg gagatggatt tcaccttccg ccagttgacc atggcgctgg
      481 cccagtcctg ggggctgccg acgctgatcg tcgccctgat caagggcgtc gacagtccgc
      541 gcgccaatct cgccaggctc ggccacgagg cggccaaggc agtgatcggc gaagccacca
      601 ccccggaact gctggcgctg ctgcaggagg tcggcgcctt gctgccggcg gtcgatctgc
      661 tggcgattgc cgagccgctg ccgctggccg atgagccgcg cgctgccttg caggcggcga
      721 ttgccgccgt caaggccgcc gcgtcaggcg agcagccggc gcagggtggc gaccaggctt
      781 gagcactgct cgtcgctgcc gacggtgatg cgcaggaact ggtcgatgcg cggcagccgg
      841 aagtggcgga cgatgatgct ctcggcgcgc agcgcctgcg cgagttcgcc ggcgtcgcgt
      901 tgcgggtggc gggcgaagat gaagttggcg gcggacggca ggacgtcgaa accgagttcg
      961 gccaactggc cgaccagcga ctcgcggctg gcaatgaccg ccgcgcagca ctgccggaag
     1021 taggcttcgt cctcgatcgc ggctacggcg ccgacgatcg ccaggcggtc gagcgggtag
     1081 gagttgaagc tgttcttgac ccgttccagc gcctcgatca ggtccgggtg gccgaccgcg
     1141 aagccgaccc gcagcccggc caaagagcgc gacttggaca gcgtgtgcac gaccagcaag
     1201 ttgtcgtatt ggcgggtcag cgggatcgcg ctggtgccgc cgaagtcgac ataggcctcg
     1261 tcgacgagca ccaccgaatc ggggttggcg gcgacgatac gctcgatcgc ggcgagcggc
     1321 agcaggcggc cggtcggggc gttcgggttg gcgatcagga tgccgccgtt ggcgaccccg
     1381 cagtagtcgg ccggatcgat cgaaaaatcg gcggcgagcg gcacggcgac gtggtcgacg
     1441 ccgtagagcc cgcaatacac cgggtagaag ctgtaggtga tgtccgggaa gaggagcggt
     1501 gcgtcgtgct tgagcagcgc ctgaaaggcg tgcgccagca cctcgtcgga accgttgccg
     1561 acgaagactt cacgcgtcgt cacgccgtga tgggcggcga tcgcctgctt caacaggtcg
     1621 gcgttggggt cagggtagag gcgcaggctg gcgccgtcgt cgccgagttc ggcacggatc
     1681 gcctcgacca cccgcggcga cggcgggaag ggatgctcgt tggtattgag cttgatcagg
     1741 ttggcgatct tgggctgttc gcccgggacg tagggggtga ggtcgcgggc gacctggctc
     1801 cagaagcggc tcatcggcta ttcacggaag gcgtaaaaat gaggcgaaat ggtatcatgc
     1861 ccctccgttc caacgatttt tagccaagtc catgcggcaa gccgaagtca ctcgcaatac
     1921 cctggaaacc cagatcaccg tcaagctcaa cctcgacggc agcggccagg gcaagttcgc
     1981 caccggcgtt cccttcctcg accacatgct cgaccagatc gcccggcacg gcctgatcga
     2041 cctcgacatc gacgccaagg gcgacctgca catcgacgcg caccataccg tcgaggacat
     2101 cggtatcacg ctcggccagg cgctggcgaa agcctggggc gaca
//
LOCUS       DAOSDX010000517         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17795_length_2143_cov_2.228448, whole genome shotgun sequence.
ACCESSION   DAOSDX010000517 DAOSDX010000000
VERSION     DAOSDX010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17795_length_2143_cov_2.228448"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>666
                     /locus_tag="PLN02_10965"
     CDS             <1..>666
                     /locus_tag="PLN02_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOI52889.1"
                     /translation="AVWLGYATLAVMLAITTTVLLYFKAELKQLSERTTPKDLNSILQ
                     FAVLSLVILPILPNASFGPYAAINPRQVWWMVVLISGLALAGYLALRIIGARHGAAVL
                     GIFGGLASSTATTMMFSRHAAEHGHLVRMSAIVILIANVMVMIRLGIIASIVAPGLIA
                     PVATVFACGIVPGVIMSLYGWKILNEAGELPMPEVKNPTELKTAVSFGLLYAVVLLAA
                     AWL"
     assembly_gap    667..676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <677..994
                     /locus_tag="PLN02_10970"
     CDS             <677..994
                     /locus_tag="PLN02_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOI52890.1"
                     /translation="VDADFIILAGNAWLQDIAGNSGLYIVALVSGLTDADASVLSTLR
                     MFNLERVLGADAVIAVTLALVANLVFKIGLVVSIGGRVLARHALPGLLAIGAGMGVGL
                     LLV"
     gene            1005..1808
                     /gene="amrB"
                     /locus_tag="PLN02_10975"
     CDS             1005..1808
                     /gene="amrB"
                     /locus_tag="PLN02_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOI52891.1"
                     /translation="MNAIRPPAVAGMFYPASAAGLRADVDRLLAAAPANTLTQQPKAL
                     IVPHAGYVYSGPTAARAYATLAPWRATIRRVVLLGPTHRVPVRGLALPASEAFATPLG
                     EVRVDAAARAALAGLPQVVVSETAHAQEHSLEVHLPFLQRALDDFSLLPLAVGHATPE
                     NVAEVLERLWGGPETLLVISSDLSHFLPYATAQQVDGETCRHILDLATDIDPHQACGA
                     FPVNGLLLAAQRHGLRPTLLDLCNSGDTAGDRQRVVGYAAFAFHEADHG"
     gene            1801..>2143
                     /gene="amrA"
                     /locus_tag="PLN02_10980"
     CDS             1801..>2143
                     /gene="amrA"
                     /locus_tag="PLN02_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOI52892.1"
                     /translation="MDELGNALLRHARAAIAEALGEPAPAAPDLAALHEPGATFVTLT
                     QGGELRGCIGSLEAHRPLLDDVCANARAAALRDPRFPPLVASELARTRVEVSLLTPAE
                     PLPVASEADACR"
BASE COUNT          297 a          821 c          665 g          350 t
ORIGIN      
        1 cggcggtctg gctgggctac gccaccttgg cggtgatgct ggcgatcacg acgacggtgc
       61 tgctctactt caaggccgaa ctgaaacagc tcagcgaacg gacgacgccg aaagatctca
      121 attccatcct gcagttcgcc gtgctctcgc tggtcatcct gcccatcctg ccgaacgcca
      181 gcttcggccc ctacgcggcg atcaacccgc gccaggtatg gtggatggtg gtgctgattt
      241 ccggcctggc gctcgccggc tacctcgcgc tgcgcatcat cggtgcccgc cacggtgccg
      301 cggtgctcgg catcttcggc ggcctggcgt cgtcgacggc gacgaccatg atgttctccc
      361 gccacgccgc cgagcacggt cacctggtcc gcatgtcggc gatcgtcatc ctgatcgcca
      421 acgtcatggt catgatccgt ctcggcatca tcgccagcat cgtcgcgccc gggctgatcg
      481 cgccggtcgc caccgttttt gcctgcggca tcgtccccgg cgtgatcatg tcgctgtacg
      541 gctggaagat cctcaacgag gccggcgagc tgccgatgcc ggaagtgaag aacccgaccg
      601 aactgaaaac cgccgtctcc ttcggcctgc tctacgccgt cgtcctgctc gccgctgcct
      661 ggctgcnnnn nnnnnngttg acgccgattt catcatcctg gccggcaacg cctggctgca
      721 ggacatcgcc ggcaacagcg gcctctacat cgtcgccctg gtttccggcc tgaccgacgc
      781 cgacgccagc gtgttgtcca cgctgcgcat gttcaacctg gaacgcgtgc tcggcgccga
      841 cgcggtgatc gccgtgacgc tggcgctggt cgccaacctg gtgttcaaga tcggcctggt
      901 ggtcagcatc ggcggccggg tgctggcccg ccacgcgctg cccggtctgc tggcaatcgg
      961 cgccggcatg ggcgtgggcc tgttgctggt gtgaaggagg aaagatgaac gcgatccgcc
     1021 cgccggccgt cgccggcatg ttctacccgg cctcggcggc cggcctgcgt gccgacgtcg
     1081 accgcctgct cgccgccgcg ccagcgaaca ccctgacgca acagccgaag gcactgatcg
     1141 tgccgcatgc cggctacgtc tattccgggc cgaccgccgc ccgcgcctac gcgacgctgg
     1201 cgccgtggcg ggcaacgatc cgccgcgtcg tgctgctcgg gccgacccac cgcgttcccg
     1261 tgcgcggtct cgccctgcct gccagcgagg cctttgccac gccgctcggc gaggtacgcg
     1321 tcgatgccgc cgcccgcgcc gcgctcgccg gtctgccgca ggtcgtcgtc agcgagacgg
     1381 cgcatgccca ggaacactcg ctggaagtcc acctgccctt cctgcagcgc gccctcgacg
     1441 acttcagcct gctgccgctg gccgtcggcc acgccacgcc ggagaatgtc gccgaagtcc
     1501 tcgaacggct atggggcgga ccggagacgc tgctcgtcat cagctccgac ctgtcgcatt
     1561 tcctgcccta cgccacggcg caacaggtcg acggcgagac ctgccgccac atcctcgatc
     1621 tggcaacgga catcgacccg caccaggcct gtggtgcctt cccggtcaac ggtctgctgc
     1681 tcgccgcgca acgccacggc ctgcgaccga ccctgctcga cctgtgcaac tccggcgaca
     1741 ccgccggcga ccgccagcgt gtcgtcggtt acgccgcctt cgccttccac gaggccgatc
     1801 atggatgaac tcggcaacgc cctgttgcgc cacgcccgcg ccgccatcgc cgaagcgctt
     1861 ggcgaaccgg cacccgccgc accagacctg gcagcgctgc acgaacccgg cgccaccttc
     1921 gtcacgctga cccagggcgg cgagctgcgc ggttgcatcg gcagcctcga agcacaccgg
     1981 ccattgctcg acgacgtgtg cgccaacgcc cgcgccgccg ccctgcgcga tccgcgcttc
     2041 ccgccgctgg tagccagcga actggcgcgc acccgcgtcg aggtctcgct actgacaccg
     2101 gccgagccgc tgccggtcgc ctccgaagcc gacgcctgcc gtc
//
LOCUS       DAOSDX010000518         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17804_length_2142_cov_3.663632, whole genome shotgun sequence.
ACCESSION   DAOSDX010000518 DAOSDX010000000
VERSION     DAOSDX010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17804_length_2142_cov_3.663632"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLN02_10985"
     CDS             complement(<1..349)
                     /locus_tag="PLN02_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOI52893.1"
                     /translation="MPQLPDLVCPAGSLPALKTAVDNGADAVYLGFKNDTNARNFAGL
                     NFDPKTMGDGVRYAHARGREVLLAINTFPQAGRVGDWHKAVDAAVDLGVDAIILADVG
                     LLDYACRRHPQQRL"
     gene            474..1376
                     /locus_tag="PLN02_10990"
     CDS             474..1376
                     /locus_tag="PLN02_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52894.1"
                     /translation="MSDNRVLVVDDEAINLMLIEDFLEQERLMLELFTDPLAAWQRLA
                     APDSDFALVVLDRMMPGMDGIEFLRRVKAERRFADVPVIMQTAASAPEQVREGLEAGA
                     YYYLTKPYAPEALISIVRAALEDRRARVSLRTLAAHIEEAQKLLVCAEYGFSTLEEIS
                     CLVPVLAGICPEPDRVAPGLSDLMVNAVEHGNLGISYREKSLLKWDGDWEGEIRKRLV
                     QPEFRERRASVRIERTASAISFRIADQGRGFDWRKFLDFDPERVFDPNGRGIAMARMT
                     SFSRLQYEGCGNVVVATVDLPAAA"
     gene            complement(1383..>2142)
                     /locus_tag="PLN02_10995"
     CDS             complement(1383..>2142)
                     /locus_tag="PLN02_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOI52895.1"
                     /translation="DGPGDEIPENLEQTPDAVPRWEPLHKPALRPYEVFGTSYVPNTS
                     VRPYKARGIASWYGKKFHGQKTSIGEPYDMFAMTAAHPTLALPSYVRVSNVQNGRSVV
                     VRVIDRGPFHASRIIDLSYTAAYKLGLVQGGSGEVEVEAIIPGQETSTTYAQVAPPAA
                     PSAAPPPAPVVETALPSGVYLQLGAFANADNADNLKLHLERELDWVDARMHILRANGL
                     HRLQLGPYASRTEAQVVADRFRSSLGYQPTIVTR"
BASE COUNT          348 a          630 c          758 g          406 t
ORIGIN      
        1 gcaggcgctg ctgcgggtgg cgtcggcagg cgtagtcgag cagcccgaca tcggccagga
       61 tgatcgcgtc cacgccgagg tctaccgcgg catcgactgc cttgtgccag tcgccgacgc
      121 ggccggcctg ggggaaggtg ttgatcgcca gcaagacctc gcggcccctg gcgtgggcat
      181 aacggacgcc gtcgcccatg gtcttcggat cgaagttgag gccggcgaaa ttgcgcgcgt
      241 tggtatcgtt cttgaagccg agatagacgg catcggcgcc attgtcgacg gcggtcttga
      301 gggccggcag gctgccggcc ggacagacga ggtcgggcag ttggggcatg gctggttcca
      361 ggcaggttaa tcaaggaagt atagcgactc gtgtcgggct gccgttgatc cgcatcaaag
      421 gccgggctcg tgctaggatt ggcgcacgta acgttcgctt ggaactcagc ttcatgtcgg
      481 ataaccgtgt tctcgtcgtc gacgacgaag ccatcaacct gatgctgatc gaggattttc
      541 tcgagcaaga acggttgatg cttgagttgt tcaccgaccc gctcgccgcc tggcagcgtc
      601 tggcggcacc cgacagcgat ttcgcactgg tcgtgctcga tcgcatgatg ccggggatgg
      661 acggcatcga gttcctgcgc cgggtcaagg cggagaggcg cttcgccgat gtgccggtga
      721 tcatgcagac ggcggcgtcg gcgccggaac aggtgcgcga gggtctggag gccggcgcct
      781 actactacct gaccaagccc tatgcgccgg aagcgctgat ctcgatcgtc cgcgcggcgc
      841 tcgaggaccg tcgcgcccgc gtttctctgc gcacgctcgc ggcgcacatc gaggaagcac
      901 agaagctact cgtctgcgcc gaatacggct tttccacgct ggaggaaatc tcttgtctgg
      961 tgccggtgct ggccggcatc tgcccggagc ccgatcgcgt cgcccccggt ctgtcggacc
     1021 tcatggtcaa tgcggtcgaa cacggcaatc tggggatttc ctaccgcgag aagtcgctgc
     1081 tcaaatggga cggcgactgg gaaggcgaaa tccgcaagcg gctcgttcag cccgaattcc
     1141 gcgagcggcg ggcaagcgtg cgcatcgagc ggacagcgtc ggcgatttcc ttccgtatcg
     1201 ccgaccaggg gcgcggtttc gactggcgca aattcctcga tttcgacccc gagcgcgtct
     1261 tcgaccccaa cggccgggga attgccatgg cgcggatgac cagtttcagc cgcctgcagt
     1321 acgaaggttg cggcaacgtc gtcgttgcca ccgtcgattt gcccgcggcg gcgtaagcgg
     1381 cttcagcggg taacgatagt cggctggtaa ccgaggctgc tgcggaagcg atcggcgacc
     1441 acctgtgcct cggtgcgcga ggcgtaaggg ccgagctgca ggcggtgcag cccgttggcg
     1501 cgcagaatgt gcatgcgggc atcgacccag tcgagttctc tttccagatg cagtttcaga
     1561 ttatcggcgt tgtcggcatt ggcgaaagcg ccaagctgca ggtatacccc ggacggcagg
     1621 gcggtttcga cgaccggtgc cggaggtggt gcggcactgg gggcggctgg cggcgccacc
     1681 tgagcatagg tggtgctggt ttcctggccg gggatgatcg cctcgacttc gacttcaccg
     1741 ctgccgccct gaaccagtcc gagcttgtag gcggcggtgt aggagaggtc gatgatgcgg
     1801 ctggcgtgga aaggaccgcg atcgatgacg cggaccacca ccgagcggcc gttctgcacg
     1861 ttgctgacgc gcacgtagga gggcagggcc agggtcgggt gggcggcggt catggcgaac
     1921 atgtcgtaag gttcgccgat cgaggtcttc tgtccgtgga acttcttgcc gtaccagctg
     1981 gcgatgccgc gcgccttgta agggcgcacg ctggtgttcg gcacataact ggtgccgaac
     2041 acttcgtagg ggcgtagcgc cggtttgtgc agcggttccc agcgcgggac cgcgtcgggg
     2101 gtttgttcaa ggttttccgg aatttcgtcg cccgggccgt cg
//
LOCUS       DAOSDX010000519         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17864_length_2137_cov_2.703170, whole genome shotgun sequence.
ACCESSION   DAOSDX010000519 DAOSDX010000000
VERSION     DAOSDX010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17864_length_2137_cov_2.703170"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLN02_11000"
     CDS             complement(<1..251)
                     /locus_tag="PLN02_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOI52896.1"
                     /translation="MDAGQPEFGKIVVFGTGLIGGSFALGLKAAGAVEEVVGFGRTPS
                     TLRVAQELGVIDRAGINPAHEIGDADIVLVATPVAQMPE"
     gene            complement(252..1340)
                     /gene="hisC"
                     /locus_tag="PLN02_11005"
     CDS             complement(252..1340)
                     /gene="hisC"
                     /locus_tag="PLN02_11005"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOI52897.1"
                     /translation="MSLTEQALSYVRAISPYQPGKPITELAREMGIPVDSIVKLASNE
                     NPLGMSPKARQAVEAALAGVERYPDQFELIAKLAARCGVAQNQVVLGNGSNDVLDLVA
                     RAFLAQGRSAVFAQHAFAVYPLATLSIGGELICVPAKDFGHDLDAMLAAIRPDTRVVW
                     IANPNNPTGNFLPYPDVRAFLEKVPADVVVVLDEAYNEYIPPDARVDTAAWVAEFPNL
                     VVCRTFSKIYGLAGLRIGYGVTSAAVADLMNRVRQPFNVNNLALAGALAALDDDEFLQ
                     ASHELNRRGMAQIVAALEKLGLDYIPSYGNFVTFKAGDGAAVNQKLLQQGVIVRPIGG
                     YGMPEWLRVTIGSEAENARFIAALEKAL"
     gene            complement(1350..>2137)
                     /gene="pheA"
                     /locus_tag="PLN02_11010"
     CDS             complement(1350..>2137)
                     /gene="pheA"
                     /locus_tag="PLN02_11010"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOI52898.1"
                     /translation="GTFSESAATKHFGHAGRLLPQASIDDVFREVESGHAHYAVVPVE
                     NSTEGAVGRTMDLLLGTPLKICGEVVLRIHQNLLSKETDLKAINRVYSHAQSLAQCHE
                     WLNRMLPRAQRISVGSNAQAAQKAAEEAGSAAIAGEAAAARYGLAKLVENIEDEPNNT
                     TRFLVLGKHDAGPSGRDKTSLIMSAPNRAGALHELLAPLSAAGVSMCRLESRPARNAL
                     WEYVYYVDVEGHRDEPAVKAALVELAGNAAYLKILGSYPVAVF"
BASE COUNT          347 a          678 c          747 g          365 t
ORIGIN      
        1 atttccggca tctgcgccac cggcgtcgcc accagcacga tgtcggcgtc gccgatctcg
       61 tgcgccgggt tgatcccggc gcggtcgatg acgcccagct cctgcgccac acgcaaggtc
      121 gacggggtgc ggccgaagcc gaccacctcc tcgacggcgc cggccgcctt gaggccgagc
      181 gcgaaggaac ctccgatcag gccggtaccg aagacgacga tcttgccgaa ctcgggctgg
      241 ccggcgtcca tttacagggc cttttccagc gccgcgatga agcgcgcgtt ctcggcttcg
      301 ctgccgatcg tcacgcgcag ccactcgggc atgccgtagc caccgatcgg ccggacgatg
      361 acgccttgct gcagcagctt ctggttcacc gcggcgccgt cgccggcctt gaaggtgacg
      421 aagttgccgt aggacgggat gtaatcaagg ccgagctttt ccagcgcggc gacgatctgc
      481 gccatgccgc gccggttgag ttcgtggctg gcctgcagga attcgtcgtc gtcgagcgcg
      541 gcgagcgcgc cggcgagcgc caggttgttg acgttgaacg gttggcggac gcggttcatc
      601 aggtcggcga cggccgccga agtgacgccg tagccgatgc gcagcccagc caggccgtag
      661 atcttggaga aggtgcggca gaccaccagg ttggggaatt cggcgaccca ggcggcggta
      721 tcgacacgtg catcgggcgg gatgtattcg ttgtaggcct cgtcgagaac gacgacgaca
      781 tcggccggca ccttttcgag gaaggcgcgc acatccggat agggcaggaa gttgccggtc
      841 gggttgttcg ggttggcgat ccagacgacg cgggtgtccg gacggatggc ggcgagcatc
      901 gcgtcgaggt cgtggccgaa atccttggcc ggcacgcaga tcagttcgcc gccaatcgac
      961 agcgtggcca gcggatagac ggcgaaagca tgctgggcga acaccgccga acgtccctga
     1021 gccaggaagg cgcgagcgac caggtcaagc acgtcgttcg agccgttacc aagcaccacc
     1081 tggttctggg caacgccgca gcgcgccgcc agcttggcga tcagctcgaa ctggtccgga
     1141 tagcgctcga cgccggccag cgccgcttcc accgcctgcc tggccttcgg gctcatgccg
     1201 agcgggttct cgttggaggc gagtttgacg atgctgtcca ccgggatgcc catctcgcgg
     1261 gccagttcgg tgatcggctt gcccggctgg tagggcgaga tggcgcggac gtaggaaagg
     1321 gcttgttcgg tgagggacat cgggattcct cagaagacgg cgaccgggta ggagccgagg
     1381 attttcaggt aggcggcgtt gccggccagt tctacgagcg cggccttgac cgctggctcg
     1441 tcgcgatgtc cttcgacatc gacgtaatag acgtattccc acaaggcgtt gcgcgccggc
     1501 cgcgattcca gccggcacat cgagacgcca gcggcggaca gtggcgccag cagttcgtgc
     1561 agggcaccgg cacgattggg tgccgacatg atcagcgagg tcttgtcgcg ccccgacgga
     1621 ccggcatcgt gcttgccgag gacgaggaag cgcgtcgtgt tgttcggttc gtcttcgatg
     1681 ttttcaacca gcttcgccag accgtaacgc gcggccgcgg cctctccggc gatcgccgcc
     1741 gaaccggctt cctcggcggc cttctgcgct gcctgcgcgt tgctgccgac cgagatgcgc
     1801 tgcgcccgcg gcagcatgcg gttgagccat tcgtgacact gcgccagcga ctgggcgtgc
     1861 gaatagacgc ggttgatcgc cttgagatcg gtttccttcg acagcaggtt ctggtggatg
     1921 cgcagcacga cctcgccgca gatcttcagc ggcgtgccaa gcagcaggtc catcgtccgg
     1981 ccgaccgcgc cttcggtcga attctcgacc ggcacgacgg cgtaatgggc atggccggac
     2041 tcgacctcgc ggaagacgtc gtcgatcgag gcctgcggca gcaggcggcc ggcgtggccg
     2101 aaatgcttgg tcgccgccga ttccgagaag gtgccga
//
LOCUS       DAOSDX010000520         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17871_length_2136_cov_4.044690, whole genome shotgun sequence.
ACCESSION   DAOSDX010000520 DAOSDX010000000
VERSION     DAOSDX010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17871_length_2136_cov_4.044690"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..296)
                     /locus_tag="PLN02_11015"
     CDS             complement(99..296)
                     /locus_tag="PLN02_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52899.1"
                     /translation="MEQEKKPYQVGATPLLLDGERAEPGNIVELTDKEAAALGDHVSP
                     AAADAASAGDESKAPKKGAKA"
     gene            complement(310..1236)
                     /locus_tag="PLN02_11020"
     CDS             complement(310..1236)
                     /locus_tag="PLN02_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52900.1"
                     /translation="MGRLSNLRVVDPVLTNLSVGYSNADLVGDVLFPFVPVDKEGGKI
                     PKFGKEAFKIYNTERALRAKSNRINPEDVDSVTVSLDEHDLEYPIDYRESDEAAFPLE
                     AHATHVVTEGIRLRHEKKVADLAQNTANYAASNKIVLAGTSRFTDKANSDPIGVFEDG
                     KEAVRGKIGKYPNTAVIGAASWKAIKQHPQFLERIKYSMKGVLTIELLKEILEVERIV
                     VGRAVYSSNTGTFGDLWSDNIVLAYVAQQRQGAERTPYEASFGYTLRKKGMPQIDKRT
                     EDGKLELVRNTDNFGVYLLGAEAGFLIADTNA"
     gene            complement(1256..>2136)
                     /locus_tag="PLN02_11025"
     CDS             complement(1256..>2136)
                     /locus_tag="PLN02_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52901.1"
                     /translation="FAELVATGRYKKISASFYLPDARNNPVPGVYYLRHVGFLGAQPP
                     AVKGLKQAEFADAEDGVVEFGDWGMEANASLWRRMREWLLAKFGQEAADQVVPDWQIE
                     SIREAARQDDDAPRTVFADPTVSPIHPPHEENHAVTPEEKAALEAENAQLKQKLAAAE
                     AEQKTAAADKRHGEHLAYAEQLVGEGKLAPKHKDAVVAFLDFSDGETALEFGEGDAKQ
                     PLASAFKSFLGDMPKVVEFGETATKGKAGQQAASNSVDYGENVDQDRLALDGKIRQYM
                     QEHKVDYASAASCVIK"
BASE COUNT          305 a          654 c          722 g          455 t
ORIGIN      
        1 accggcggcg cacacggcgc cggtcagacc gacgaagcgc agccggctca gggcggctgc
       61 agccgtgacc gaggtggtca ggcaggattg ttgggtcttc acgccttagc tcctttcttg
      121 ggggccttcg attcgtcgcc ggcactggcc gcatcggcgg ccgccgggga cacgtggtcg
      181 ccgagggcgg ccgcttcctt gtcggtcagc tcgacgatgt tgccgggttc ggcgcgctcg
      241 ccgtcgagca gcagcggcgt ggcgccgacc tgatagggtt tcttttcctg ttccatcagc
      301 ggctcccggt taggcgttgg tgtcggcgat caggaagccg gcttcggcac cgagcagata
      361 cacaccgaag ttgtcggtgt tgcgcaccag ctccagcttg ccgtcctcgg tacgcttgtc
      421 gatctgcggc atgcccttct tgcgcagcgt gtagccgaag gaggcttcgt aaggcgtgcg
      481 ctcggcgccc tggcgctgct gggcaacgta ggcgagcacg atgttgtcgc tccacaggtc
      541 gccaaaggtg cccgtgttgc tggaatagac ggctcgtccg accacgatgc gctcgacctc
      601 caggatttcc ttgagcagtt cgatggtcag cacacccttc atgctgtact tgatgcgctc
      661 caggaactgc gggtgctgct tgatcgcctt ccaggaggcg gcgccaatga cagcggtgtt
      721 cgggtacttg ccgatcttgc cgcgcacggc ttccttgccg tcctcgaaga cgccgatcgg
      781 gtcggagttg gccttgtcgg tgaagcggct ggtgccggcc aggacgatct tgttgctggc
      841 cgcgtagttc gcggtgttct gcgccagatc ggccaccttc ttctcgtggc gcaggcggat
      901 gccctcggtc acgacgtggg tggcgtgcgc ttccaagggg aaggccgcct cgtcgctctc
      961 gcggtagtcg atcgggtatt ccagatcgtg ctcgtcgagg gagacggtga cgctgtccac
     1021 gtcctcggga ttgatgcggt tggacttggc ccgcagcgcc cgttcggtgt tgtaaatctt
     1081 gaaggcttcc ttgccgaact tcggaatctt gccgccttcc ttgtccaccg gaacgaaggg
     1141 aaagaggaca tcgcctacca ggtcggcgtt gctgtagccg accgacaggt tggtcagaac
     1201 cgggtcaacg acccgcaggt tggataaacg gcccattgaa atctcctggg ttgggttact
     1261 tgatcacgca ggaggcagcg ctggcgtaat ccaccttgtg ctcctgcatg tactggcgaa
     1321 tcttcccgtc gagggcgagg cggtcctgat cgacgttctc gccgtagtcc accgagttgc
     1381 tggcggcttg ctggccggcc ttgcccttgg tggcggtttc gccgaactcg acaaccttgg
     1441 gcatgtcgcc caggaaggac ttgaaggcac tggcaagcgg ctgcttggca tcgccctcgc
     1501 cgaactccag ggcggtctcg ccatccgaga aatccaggaa ggcgaccacg gcgtccttgt
     1561 gcttgggcgc gagcttgcct tcgccgacga gctgctcggc gtaggccagg tgttcgccat
     1621 ggcgcttatc cgccgctgct gtcttctgct cggcttcggc cgccgccagt ttctgtttta
     1681 gctgggcgtt ttcggcctcc agtgcggcct tttcctcggg tgtcactgca tggttctcct
     1741 cgtggggtgg gtggatcggg gaaacggtgg gatcagcgaa gacggtgcgc ggggcgtcgt
     1801 cgtcctggcg cgcggcttct cggatggatt cgatctgcca gtcgggcacg acttggtcgg
     1861 cggcctcctg gccgaacttg gccagcagcc actcacgcat gcggcgccag agggaggcgt
     1921 tggcctccat tccccagtcg ccgaactcga ctacgccgtc ttcggcatcg gcaaactcgg
     1981 cctgcttgag gcccttcacc gctggcggct gggcacccag gaaaccgacg tggcgcaggt
     2041 aatagacgcc gggcaccggg ttgttgcggg cgtcgggcag atagaagctg gcgctgatct
     2101 tcttgtagcg gccggtggcc accaactcgg cgaaat
//
LOCUS       DAOSDX010000521         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17904_length_2133_cov_3.670837, whole genome shotgun sequence.
ACCESSION   DAOSDX010000521 DAOSDX010000000
VERSION     DAOSDX010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17904_length_2133_cov_3.670837"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLN02_11030"
     CDS             complement(<1..825)
                     /locus_tag="PLN02_11030"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HOI52902.1"
                     /translation="MMQLSAGEILQLSPDESSSKAAKGLVVPAKWPRLEYDERAIWGE
                     CQGSGSKPYQVQVDKSGPAFRCSCPSRKFPCKHGLALLLLMAQDASLFSAATAPAWVA
                     EWLAGRQQRAEKQEARIAEKRDAAPDPQAATRREDNRRQRMAGGLEELERWLNDRLRQ
                     GLAQLPGQTAIWEDLARRMVDAQLPGLAFRLRQLASRVGRDEAWPAQVLGGLGQLRLL
                     IDAFRRLDSLPDAIRQDVRSALGTNLDRDEVLAGGERLRDDWLVLGQSIEEEGNLWS"
     gene            complement(1093..>2133)
                     /locus_tag="PLN02_11035"
     CDS             complement(1093..>2133)
                     /locus_tag="PLN02_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HOI52903.1"
                     /translation="GKDFLVVGSVRLGNDGSIETRFRLHDVQAGKEMGGQSLVIGRQH
                     LHRVAHRISDFIYEKLTGKQGPLSSRLAYVTKSTDRYLLMIADADGDYPQTAMVSKEP
                     ITSLAWAPEGRRIALVSHEAINPATGLSHPMVFMLSLASGQRQLLFNPLLGETVAGIT
                     WSPDGTSVAVMSRKGDRSALVVINVDGKEEKPISISRSEDANPAFSADSQYIYFDSNR
                     SGSQQIYRIPVRSGSPEQVFKGANESLAPSPSRDGKTLAYIARSGFQERLAVLDLASG
                     TSTVLQESPASQRLSFAANGDFFLFSEKSNGTSILKAVSMETLGSISWTTASEAANST
                     DFVLSPAENKEP"
BASE COUNT          410 a          637 c          635 g          451 t
ORIGIN      
        1 cgaccaaaga ttgccttctt cctcgatcga ctggccgagt accagccagt cgtcgcgcag
       61 gcgttcgccg ccggccagca cttcgtcgcg gtcaaggttg gtgccgaggg cgctgcgcac
      121 atcctggcgg atggcgtcgg gcaggctgtc gaggcggcgg aaggcatcga tcagcaggcg
      181 cagttgcccg agtccgccca gcacctgcgc cggccaggct tcgtcccggc cgacccggct
      241 ggcgagctgg cgcaggcgaa aggcgaggcc gggcagttgg gcgtcgacca tgcgccgggc
      301 gaggtcttcc cagattgccg tttgccccgg cagttgcgcc aggccctggc gcagccggtc
      361 gttgagccag cgctccagtt cttcgagccc gccggccatg cgctggcggc ggttgtcctc
      421 gcgccgagtg gctgcttgcg ggtcgggcgc cgcgtcgcgt ttttcggcga tcctggcttc
      481 ctgtttttcg gcgcgttgct ggcgcccggc cagccattcg gcaacccagg ccggcgccgt
      541 ggcggccgag aacagggagg catcctgcgc catcagcagc aacagcgcca ggccgtgttt
      601 acagggaaac ttgcgactgg gacaggaaca gcggaaagcc ggcccggact tgtccacctg
      661 cacctgataa ggcttgctgc cgctgccctg gcattcaccc cagatggccc gttcgtcgta
      721 ttccagacgc ggccatttgg ccggaaccac cagccccttg gccgccttgc tcgaagactc
      781 gtcaggcgaa agttgcagga tttcgccggc gcttagctgc atcatcgaat tctcgttggt
      841 caggagtggc gaggatttta ggtgtttggc atgggctgcg gcaagcgtcg tcgactggga
      901 gagggcgggt cggtcggctg acgcggaaaa caggctgaaa tctttgtgcg aaatcgctag
      961 tggcagacca cagaatcgcg ggtacttgac cgactcgaat cgactcgttg atgtcgttcg
     1021 gctcttcgaa aagctgtcat tcaaacacgc caaacatgct cttggctcga agcgcgccta
     1081 accttcgaaa ggctacggct ccttgttttc ggcaggactc aacacaaaat ctgtgctgtt
     1141 cgctgcttcc gatgccgttg tccacgatat cgagccaagc gtttccatgg aaaccgcttt
     1201 caagatggaa gtaccgttag acttttcgga gaagagaaag aagtcaccgt tcgcagcaaa
     1261 gctcaagcgt tgactggcgg gcgactcctg taaaacggtg ctggtaccgc tggcaaggtc
     1321 cagcactgcg aggcgttcct ggaagcctga gcgagcgatg taggctagtg tttttccatc
     1381 tcgggatggg ctgggagcga gactttcgtt ggcgcccttg aatacctgct ccggcgaccc
     1441 acttcgaacg ggtatgcgat agatttgctg gctaccactc cgatttgaat cgaaatatat
     1501 gtactggcta tcggccgaaa atgccggatt ggcatcctcg cttctggaga tgctaattgg
     1561 cttttcttcc ttgccatcaa cattgatgac aactagtgcg gaacggtctc cctttcgcga
     1621 catgactgcc acactggtac catctggcga ccatgtgatc cctgccaccg tttcacccaa
     1681 cagcggatta aacaacaact gccgctgccc cgaagccaaa gagagcatga acaccatggg
     1741 gtgggagagt cccgtcgcag gatttattgc ctcgtgcgaa acaagcgcaa tgcgccttcc
     1801 ttccggtgcc caggctagcg aagttatcgg ctctttagaa accatggccg tctgtgggta
     1861 gtcaccgtcg gcatcggcga tcatcaacaa atagcgatcc gtactcttgg tgacgtaagc
     1921 taaacgggag gatagcgggc cctgtttgcc cgtcagcttc tcataaatga agtcgcttat
     1981 tcgatgtgcg acgcgatgga ggtgctgcct cccaatgacc aaactctggc ctcccatttc
     2041 cttacctgct tggacatcgt gcaaccgaaa gcgcgtctca atgctcccgt cattcccaag
     2101 gcgaacgctg ccgaccacca aaaagtcttt tcc
//
LOCUS       DAOSDX010000522         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17927_length_2131_cov_3.359345, whole genome shotgun sequence.
ACCESSION   DAOSDX010000522 DAOSDX010000000
VERSION     DAOSDX010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17927_length_2131_cov_3.359345"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /locus_tag="PLN02_11040"
     CDS             complement(<1..1826)
                     /locus_tag="PLN02_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HOI52904.1"
                     /translation="MFNKILIANRGEIACRVIKTARKMGIKTVAVYSEADKDALFVDM
                     ADEGVCIGPAASKESYLVADKIIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKF
                     IGPKHYSIAKMGDKIESKKLAIEAKVNTIPGHNDAIDGPDAAVEIAKKIGYPVMIKAS
                     AGGGGKGLRVAYNDAEAHEGFSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSH
                     GNYVYLNERDCSIQRRHQKVIEEAPSPFVDPEMRKAMGEQAVALARAVNYESAGTVEF
                     VVSGATKEFYFLEMNTRLQVEHPVTELITGLDLVEQMIRVAYGEKLPLTQADVKINGW
                     SMECRINAEDPFRGFLPSTGRLVKFLPPAESTDAQGTTRVDTGVYDGGEISMFYDSMI
                     AKLIVHGKDRNAAIARMRDALNGFVIRGISSNIPFQAALMQHPVFHSGIFDTGFIPKH
                     YPTGFDASMVPHDDPALLVSVAAYVYRAFTDRSASITGQLQGHERIVGDQWMVVRLNG
                     EQKENHPVTARPIPGGYHVEYAGQPYEILSDWKLGESLFTGTCNGQEFTLQVERHKTK
                     YSLFHWGTRADFMVMSARAAELLALMPEKPAPDLSKFLLSPMP"
     gene            complement(1848..>2131)
                     /locus_tag="PLN02_11045"
     CDS             complement(1848..>2131)
                     /locus_tag="PLN02_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOI52905.1"
                     /translation="VNLAWPTAEIAVMGPKGAVEIIFREEKNDPAKLAQREAEYKEKF
                     ANPFVAGARGFIDDVIMPHATRKRIARSLAMLRDKKLENPWRKHGNIPL"
BASE COUNT          347 a          615 c          721 g          448 t
ORIGIN      
        1 ggcatcggcg agagcaggaa cttcgacaga tcgggcgccg gcttctccgg catcagtgcg
       61 agcagttcgg cggcgcgggc gctcatcacc atgaagtcgg cgcgcgtacc ccagtggaac
      121 agcgagtact tggtcttgtg ccgctcgacc tgcagcgtga actcctgacc gttgcaggtg
      181 ccggtgaaca gcgactcgcc gagcttccag tcggacagga tttcgtaggg ctggccggcg
      241 tattcgacgt gatagccgcc cgggatcggg cgtgccgtga ccgggtggtt ttccttctgc
      301 tcgccgttca ggcgcaccac catccactgg tcgccgacga tgcgttcgtg gccttgcaac
      361 tggccggtga tcgacgccga gcggtcggtg aaggcgcggt agacgtaggc ggcgaccgaa
      421 accaggagcg ccgggtcatc gtgcggcacc atcgaggcgt cgaaaccggt cgggtagtgc
      481 ttggggatga agccggtgtc gaagatgccg gaatggaaga ccgggtgctg catgagcgcg
      541 gcctggaacg gaatgttcga agaaatgccg cggatcacga agccgttcag cgcgtcgcgc
      601 atgcgggcga tggcggcgtt gcggtccttg ccgtgcacga tcagcttggc gatcatcgag
      661 tcgtagaaca tcgagatttc gccgccgtcg tagacgccag tgtcgacgcg cgtcgtgccc
      721 tgggcgtcgg tcgattcggc cggcggcagg aacttgacca gacggccggt ggagggcagg
      781 aagccgcgga acgggtcttc ggcgttgatc cggcattcca tggaccagcc gttgatcttg
      841 acgtcggcct gggtcagcgg cagcttttcg ccgtaggcga cgcggatcat ctgctcgacc
      901 aggtcgaggc cggtgatcag ttcggtgacc gggtgttcga cctgcaggcg ggtgttcatt
      961 tccaggaagt agaactcctt ggtcgccccg gagacgacga actcgaccgt cccggccgac
     1021 tcgtaattca ccgcgcgggc cagggccacg gcctgttcgc ccatcgcctt gcgcatttcc
     1081 ggatcgacga aggggctcgg tgcctcttcg atgaccttct ggtgacgacg ctggatcgag
     1141 cagtcgcgct cgttcaggta cacgtagttg ccgtggctgt cgccgagcac ctggatttcg
     1201 atgtgacgcg gttcgagcac gtacttttcg atgaagacgc ggtcgtcacc gaaggagtta
     1261 cgggcttcat tgacgcagga ggagaaacct tcgtgggctt cggcatcgtt gtaggcaacg
     1321 cgcagaccct tgccgccgcc gccggcggag gccttgatca tcaccgggta gccgatcttc
     1381 ttggcgatct ccacggcggc atccgggccg tcgatggcat cgttgtgacc ggggatggtg
     1441 ttgaccttgg cttcgatcgc gagcttcttg gactcgatct tgtcgcccat cttggcgatc
     1501 gagtagtgct tcggcccgat gaacttgatg ccttcttctt cgagacggcg cgaaaactcg
     1561 gcgttttccg acaggaagcc gtagcccggg tgcaccgctt cggcgccggt ctgcttgcag
     1621 gcggcgatga tcttgtcggc aaccagatag gattccttgg aagccgccgg gccgatgcag
     1681 acaccctcgt cggccatatc gacgaacagg gcgtccttgt cggcctcgga atagacggcg
     1741 acagtcttga tgcccatctt gcgggcggtc ttgatgacgc ggcaggcaat ttcaccgcgg
     1801 ttggcgatca gaattttgtt gaacatgttc ctttttctcc ctgacgctta cagaggaatg
     1861 ttgccgtgct tgcgccacgg attttcgagt tttttgtcgc gcagcatggc gagcgagcgg
     1921 gcgatccgct tgcgcgttgc gtgcggcatg atcacgtcgt cgatgaagcc acgggcgccg
     1981 gcgacgaacg ggttggcgaa cttttccttg tactcggctt cgcgctgggc gagcttggcc
     2041 ggatcattct tttcttcacg gaagatgatt tcgaccgcgc ctttggggcc catgacggcg
     2101 atttcggcag tcggccaggc caggttgacg t
//
LOCUS       DAOSDX010000523         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17955_length_2128_cov_3.017849, whole genome shotgun sequence.
ACCESSION   DAOSDX010000523 DAOSDX010000000
VERSION     DAOSDX010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17955_length_2128_cov_3.017849"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2128)
                     /gene="tssM"
                     /locus_tag="PLN02_11050"
     CDS             complement(<1..>2128)
                     /gene="tssM"
                     /locus_tag="PLN02_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HOI52906.1"
                     /translation="FFAPISQAIEERNILRFGDKSLASRGKRMLRWGISVVALCLLSV
                     WLVLTYFDQRSYIDLIWAQFGEGKRLAQQLADTPSADIALMDVSSQMRYAGNQLGANF
                     SMIPSTFFEHSRIRNVAYETYRRHLFKSFMPEMFKYVTVQLKSEMDGGTGDVYETLRL
                     YLMLVKPERRNPEIAVRWFEKRWDKIAPANSSDEEKSVFLGHVAATFSPPFLPAAPAD
                     ENLLTQARAVASGLPSAIRVIDNVKAQALPESVEDVSLSRAAGLNAPMLLRMRSDTPT
                     TSPVISGWYTRAGYHDVFMPRLMKSAREIFEEENWVLRDENGAKGNAFDVEKSVQDMS
                     DAARRQFLQDYIQHWRGFINDITVRRYSSMDDAAWLAKAMMDPQSALAQLVRFIGRET
                     SLTGNYEGDVDSWIDRQKLNLERTRRAVVGEISGQHYRYRVLPEHVVDDYFNSLRRMA
                     ITLTPNSGVETAASPLSRLFEPLYRQISVVNGAMQVGQLMPEYDAFRRMRADAARQPE
                     PIRGIMLDLIESGSNATAQKSSAELTKGATGASKSICPQTVSRYPLVRSSGRDVGSED
                     FRRLFGPRGVLDSYFNEELAQYVDTSNVPWRAKRVDGLAGSLVNPNIIRSYELARSIR
                     DNFFNESGQLGFSVIVRAVDMDASVTEALFDIGGEQLRYAHGASAPKKIEWSPQRSSM
                     VVRLHMRSVDGRTQQMEFTGPWAIFR"
BASE COUNT          379 a          676 c          684 g          389 t
ORIGIN      
        1 aagcggaaaa tcgcccacgg cccggtgaat tccatctgct gggtgcggcc atcgacggag
       61 cgcatgtgca ggcggaccac catgctcgaa cgctgcggcg accactcgat cttcttcggc
      121 gccgaggcgc cgtgcgcgta gcgcaattgc tcaccaccga tatcgaacag cgcttcggtg
      181 acgctggcgt ccatatcgac ggcgcggacg atgaccgaaa aaccgagctg accgctctcg
      241 ttgaagaagt tgtcgcggat cgagcgggcc agttcatacg aacggatgat gttcggattg
      301 accaggctgc cggccaggcc atcgacccgc ttcgcccgcc acggaacgtt cgaggtatcg
      361 acgtattgcg ccagttcctc gttgaagtag ctgtcgagca ccccgcgcgg cccgaacagg
      421 cggcggaagt cctcggagcc gacatcgcgc cccgaactgc ggaccagcgg atagcgggag
      481 accgtttgcg gacagatcga cttcgacgcc ccggtcgccc ccttggtcag ttcggcgctc
      541 gacttctgtg ccgtcgcgtt gctgcccgac tcgatcaggt caagcatgat gccgcggatc
      601 ggctccggct gccgcgcggc gtcggcacgc atgcggcgga aggcgtcgta ctcgggcatc
      661 agttggccga cctgcatcgc accattgacc accgaaattt gccgatagag cggttcgaac
      721 aggcggctga gcggactggc cgccgtttcc accccggagt tcggggtcag cgtgatggcc
      781 atgcggcgca acgagttgaa gtagtcgtct acaacatgtt ccggcagcac gcggtagcga
      841 tagtgctggc cgctgatctc gccgaccacg gcgcgccgcg tccgctccag gttgagcttc
      901 tgacgatcga tccagctatc gacatcgcct tcgtagttgc cggtcagcga ggtttcgcgg
      961 ccgatgaagc gaaccagctg ggccagtgcc gactgcgggt ccatcatcgc cttggccagc
     1021 caggcggcgt catccatact gctgtagcgg cggaccgtga tgtcgttgat gaagccacgc
     1081 cagtgctgga tgtagtcctg caggaactgg cggcgcgccg cgtcggacat gtcctggacg
     1141 ctcttctcga catcgaaggc attgcccttg gcgccgttct cgtcgcgcag cacccagttt
     1201 tcctcctcga agatctcacg cgcgctcttc atcagccgcg gcatgaagac gtcgtggtag
     1261 ccggcacggg tataccaacc actgatgacc ggcgaggtgg tcggcgtatc gctgcgcatg
     1321 cgcaacagca tcggcgcgtt gagaccggcg gcgcgcgaca gtgacacgtc ctcgacactt
     1381 tccggcaagg cctgcgcctt gacgttgtcg atcacccgga tcgcgctggg cagaccgctg
     1441 gccaccgccc gcgcctgcgt cagcaggttc tcgtcggccg gtgcggcggg caggaagggc
     1501 ggcgagaagg tggcggcgac gtggccgagg aagacacttt tttcttcatc gctggaattc
     1561 gccggagcga tcttgtccca gcgtttctcg aaccagcgga cggcgatttc cggattccgc
     1621 cgctccggct tgaccagcat caggtacagg cgcagcgtct cgtagacgtc accggtgccg
     1681 ccatccatct cgctcttcaa ctgcacggtg acgtacttga acatctccgg catgaagctc
     1741 ttgaacaggt ggcgacgata ggtttcgtag gcgacgttgc ggatccgcga gtgctcgaag
     1801 aaggtcgacg ggatcatcga gaagttggcg cccagctgat tgccggcgta gcgcatctgg
     1861 ctggagacgt ccatcagcgc aatgtcggcg ctcggcgtat cggccagttg ctgggccagc
     1921 cgcttgcctt caccgaattg cgcccagatc aggtcgatgt agctgcgctg atcgaaatag
     1981 gtcagcacca accagaccga caacaggcac agggcgacga cgctgatccc ccagcgcagc
     2041 atccgcttgc cgcgggaagc cagcgacttg tcgccgaagc gcaggatgtt gcgctcttcg
     2101 atcgcctggc tgatcggcgc gaagaagg
//
LOCUS       DAOSDX010000524         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_17968_length_2127_cov_3.562259, whole genome shotgun sequence.
ACCESSION   DAOSDX010000524 DAOSDX010000000
VERSION     DAOSDX010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17968_length_2127_cov_3.562259"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /gene="murD"
                     /locus_tag="PLN02_11055"
     CDS             <1..976
                     /gene="murD"
                     /locus_tag="PLN02_11055"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOI52907.1"
                     /translation="TAHLLNGAGVPAIACGNISPSALDALMDAQDAGTLPALWVVELS
                     SFQLETTHHLNAAAATVLNISEDHLDRYDGSLANYAAAKSRVFQGKGTMILNRDDDWS
                     MANGRCGRKMVTFGLSPAPRAVDYGYVDGAIVRGKEAMVAAADLQLSGLHNVANAMAA
                     LALAEAVGVAPQRLVEPLREFRGLPHRVETVAEIGGVLYVDDSKGTNVGATLAAIEGM
                     GRKVAIVLGGDGKGQDFSPLKAALAAHGRAVALIGRDAAAIGMAIEGCGVPTVIVGDM
                     EAAVRWLAARAEPGDCVLLSPACASLDMYRNYAHRAQAFIDAVGGLQP"
     gene            976..>2127
                     /gene="ftsW"
                     /locus_tag="PLN02_11060"
     CDS             976..>2127
                     /gene="ftsW"
                     /locus_tag="PLN02_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOI52908.1"
                     /translation="MLSALNSPRQQMAEIDHALLWSALLLLLGGLVMVYSASIATAEA
                     GRFTNHQPAYYLIRHAIFLCIGLVAAAVAFQLPLTAWQKYAPVLFLVGVVLLAIVLIP
                     GIGRDVNGARRWLPLGFANLQPSELMKLFAVLYAADYTIRKINVMHDLKQAFLPMFGA
                     MALVGILLLKEPDFGAFVVIISIAMGILFLGGLKARMFVLLIVGLLIAFAIMIIVSPY
                     RRDRIFGFMDPWSDAFGRGYQLSHSLIAFGRGELFGVGLGASVEKLFYLPEAHTDFLL
                     AVIAEELGFVGVLTVIALFAVLVQRAFAIGRQCVQLDRLYPALVAMGIGIWLGVQSFI
                     NMGVNVGLLPTKGLTLPLMSFGGSGILANCVALAILLRVDWENRQLMRGG"
BASE COUNT          297 a          737 c          690 g          403 t
ORIGIN      
        1 gaccgcccac ctgctcaacg gcgccggtgt gccggcgatt gcctgcggca acatctcgcc
       61 gtcggcactc gacgcgctga tggatgcgca ggacgccggg acgctgccgg cgctctgggt
      121 cgtcgagctg tcgagcttcc agctcgaaac gacgcatcac ctgaacgccg cggcggcgac
      181 cgtgctcaat atctccgagg atcacctcga ccgctacgac ggtagtctgg ccaattacgc
      241 cgccgccaag tcgcgcgttt tccagggcaa gggaacgatg atcctcaacc gcgacgacga
      301 ttggtcgatg gccaacggcc gctgcgggcg caagatggtc actttcggcc tgtcgccggc
      361 accgcgcgcg gtcgattacg gctatgtcga cggcgccatc gtgcgcggca aggaagccat
      421 ggtcgccgct gccgacctgc agctctccgg cctgcacaac gtcgccaacg caatggcggc
      481 gctggcgctg gccgaggcgg tcggcgtcgc gccacaacgc ctggttgaac cgctgcggga
      541 atttcgcggc ctgccgcacc gcgtcgagac ggtggccgag atcggcggcg tgctctatgt
      601 ggacgactcc aagggcacca atgtcggcgc cacgctggcg gcgatcgaag gcatggggcg
      661 caaggtcgcc atcgtcctcg gcggcgatgg caaaggccag gacttttcgc cgctgaaggc
      721 ggcgcttgcc gcgcatggcc gggcggtggc gctgatcggc cgcgacgcag cggcgatcgg
      781 catggcgatc gaaggctgcg gcgtaccgac cgtcatcgtc ggcgacatgg aagccgccgt
      841 gcgctggctg gccgcccgcg ccgagccggg cgactgcgtg ctgctgtcgc cggcctgcgc
      901 cagcctcgac atgtaccgca actacgcgca ccgggcgcag gccttcatcg acgccgtcgg
      961 aggcttgcag ccgtgatgct ctccgcgctc aactcgccgc gccagcaaat ggcggagatt
     1021 gaccacgccc tgctctggtc ggcgctgctg ctcctgctcg gcggcctggt gatggtctat
     1081 tcggcatcga tcgccaccgc cgaggccggt cgtttcacca accaccagcc ggcctactac
     1141 ctgatccgcc acgcgatctt cctgtgcatc ggcctggttg ccgccgcggt cgctttccag
     1201 ttgccgttga ccgcctggca gaagtatgcg cccgtccttt tcctggtcgg cgtcgtcctg
     1261 ctggcgatcg tcctcattcc cggcatcggc cgcgacgtca atggcgcccg ccgctggctg
     1321 ccgctcggct tcgccaacct gcagccgtcg gagctgatga agctgttcgc cgtcctctac
     1381 gccgccgact acacgatacg caagatcaac gtcatgcacg acctgaagca ggccttcctg
     1441 ccgatgttcg gggcgatggc gctggtcggc atcctgctgc tcaaggagcc ggatttcggc
     1501 gccttcgtcg tcatcatctc gatcgccatg ggcatcctct tcctcggtgg tctcaaggcg
     1561 cgcatgttcg tgctgctgat cgtcggcctg ctgatcgcct tcgcgatcat gatcatcgtc
     1621 tcgccctacc ggcgcgaccg catcttcggc ttcatggacc cctggtccga tgccttcggc
     1681 cgcggctacc agctgtcgca ctcgctgatc gccttcggcc gtggtgaact gttcggcgtc
     1741 ggtctcggcg ccagtgtcga gaagctgttc tacctgccgg aagcgcatac cgacttcctg
     1801 ctcgcggtga ttgccgagga actcggcttc gtcggcgttc tgaccgtgat cgcgctgttc
     1861 gccgtgctcg tgcagcgtgc cttcgccatc ggccgccagt gcgtgcagct cgaccgtctc
     1921 tacccggcgc tggtggcgat gggcatcggc atctggctgg gcgtccagtc cttcatcaac
     1981 atgggcgtca atgtcggcct cttgccgacc aagggcctga ccctgccgct gatgagcttc
     2041 ggcggttcgg gcatcctcgc caactgcgtg gcgctggcca ttctcctgcg ggtggattgg
     2101 gagaacaggc aattgatgcg cggaggc
//
LOCUS       DAOSDX010000525         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18012_length_2122_cov_2.282535, whole genome shotgun sequence.
ACCESSION   DAOSDX010000525 DAOSDX010000000
VERSION     DAOSDX010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18012_length_2122_cov_2.282535"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..1617
                     /locus_tag="PLN02_11065"
     CDS             187..1617
                     /locus_tag="PLN02_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI52909.1"
                     /translation="MDFIARLTEFADRPLIQADDASVHRYASVLELAAAFERAGCARQ
                     LVFCLAGNTPGALLGYLALLRCGAVPLMLNSSLPAPQLNALIAAYRPAFHWLERQRAA
                     ELAGTREAAAYADHVLLGSATPADYAIHPDLALLVATSGSTGSPMFVRQSRRNLAANA
                     AAIAEYLAIGPDERPITTLPLSYTYGLSIIHSHVLHGCPIALTGKSFFDRGFWDFFRE
                     VAPTSFGGVPYHYEMLRKLRFWRMTLPGLRTLTQAGGRMAPEIALEMALEARQRGLRY
                     YTMYGQAEATARMSYLEPEMSIEKAGSIGRAIPGGSFSLEDEAGNELSDAAASGQLVY
                     RGANVSLGYATGYADLARGDDNQGCLRTGDLARRDSDGYYYITGRLKRFLKLFGHRIN
                     LQDVEDMLRTRGHDAACGGEDDHLVIYLNEADGADAGDLKRQLAETLRAPPSAIAVVG
                     VAALPRNDAGKLRYAELAGLAGKTLA"
     gene            1614..>2122
                     /locus_tag="PLN02_11070"
     CDS             1614..>2122
                     /locus_tag="PLN02_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HOI52910.1"
                     /translation="MTADDYLAELAQWLEIPPFALAAGDKTPRLAGALARLTQYHCRH
                     CPAYARLLDIFGDDATAAAGLADIPFLPVRLFKEYELASVAPDQVFKTMTSSGTSGQA
                     VSRIFLDRATAAGQSKVLARLMAELIGRQRLPMLIVDAPGVLRDRQRFSARGAAILGF
                     SLFGRDLTYA"
BASE COUNT          346 a          795 c          656 g          325 t
ORIGIN      
        1 accagtttac ctatcaatgc gttccggcct gggattcggt cggccacatg ggcatgatcg
       61 cggcgctgga agataccttc gggatcatga tggaaaccga cgacatcgtc gatttctcgt
      121 cctacaccaa gggaatggaa ctgctcggca agtacggcgt gaccttctga ggcagttggc
      181 gtcggcatgg acttcatcgc ccgtctaacc gagttcgccg accggccgct gatccaggcc
      241 gacgacgcga gcgtccatcg ctacgccagc gtgctcgaac tggcggccgc cttcgaacgg
      301 gccggctgcg ccaggcaact ggtcttctgc ctggccggca acacgccggg cgctctgctc
      361 ggctacctgg cactgctgcg ctgcggggcg gtaccgctga tgctcaacag cagcctgccc
      421 gccccccagt tgaacgcgct gatcgccgcc taccggccgg ccttccactg gctggagcgg
      481 cagcgcgccg cggaactcgc cggcacccgg gaagccgccg cctatgccga ccatgtcctg
      541 ctcggcagcg caacgccggc cgactacgcg atccacccgg accttgccct gctggtcgcc
      601 acttccggca gcaccggcag cccgatgttc gtccgccaat cccggcgcaa cctcgccgcc
      661 aacgccgcgg cgatcgccga atatctcgcc atcggtcccg acgagcggcc gatcaccacc
      721 ctgccgctat cctacaccta cggcctgtcg atcatccaca gccatgtcct gcacggctgc
      781 ccgatcgccc tgaccggcaa aagcttcttc gaccgcggct tctgggactt cttccgggaa
      841 gtcgcgccga cctcgttcgg cggcgttccc tatcactacg aaatgctgcg caagctgcgt
      901 ttctggcgca tgacgctgcc cggcctgcgc acgctgaccc aggccggcgg tcgcatggcg
      961 ccggaaatcg ccctcgaaat ggcgctcgaa gcccgccagc ggggcctgcg ctactacacg
     1021 atgtacggcc aggccgaggc gacggcgcgc atgtcctacc tcgaaccgga aatgagcatc
     1081 gagaaggcgg gcagcatcgg ccgcgccatc cccggcggca gcttcagcct ggaagacgag
     1141 gcagggaacg aactgagcga cgccgcagcc agcggtcaac tggtctatcg cggcgccaac
     1201 gtcagcctcg gctacgcgac gggttacgcc gacctggcgc gcggcgacga caaccagggc
     1261 tgcctgcgca ccggcgacct cgcccgccgc gacagcgacg gctattacta catcaccggc
     1321 cgcctgaagc gcttcctcaa gctgttcggc caccgcatca acctgcagga cgtcgaggac
     1381 atgctgcgca cccggggcca cgacgcggcc tgcggcggcg aagacgatca tctggtgatc
     1441 tacctgaacg aagccgacgg cgccgacgcc ggcgacctca aacgccagct tgccgagacc
     1501 ctgcgcgcgc cgccttcggc catcgccgtc gtcggtgtgg cggcgctgcc gcgcaacgat
     1561 gccggcaagc tgcgctatgc cgaactggcc ggcctggcgg gaaaaaccct ggcatgacgg
     1621 cggacgacta cctggccgag ctggcgcagt ggctggaaat cccgcccttc gcgctggctg
     1681 ccggcgacaa gacgccccgc ctcgccggcg ccctggcccg cctgacgcaa taccattgcc
     1741 gccactgccc ggcctacgcg cgcctgctcg acattttcgg cgatgacgcg acagccgccg
     1801 ccggccttgc cgacattccc ttcctaccgg tccggctgtt caaggaatac gaactggcca
     1861 gcgtcgctcc cgaccaagtg ttcaagacga tgacctcgtc cggcaccagc ggccaggcgg
     1921 tttcccggat cttcctcgac cgcgcgacgg cggccgggca gagcaaggtg ctggcccggc
     1981 tgatggccga actgatcggc cgccagcggc tgcccatgct gatcgtcgac gcgcccggcg
     2041 tgctcaggga ccgccagcgc ttctcggcgc gcggcgcggc aatcctcggc ttctcgctgt
     2101 tcggccgcga cctcacctat gc
//
LOCUS       DAOSDX010000526         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18033_length_2119_cov_2.476260, whole genome shotgun sequence.
ACCESSION   DAOSDX010000526 DAOSDX010000000
VERSION     DAOSDX010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18033_length_2119_cov_2.476260"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLN02_11075"
     CDS             <1..444
                     /locus_tag="PLN02_11075"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOI52911.1"
                     /translation="ASLQAGRYYAHPRNAFWPIMGELLGFSPDLPYAARLLALQGAGI
                     ALWDVIAGCRRRGSLDADIVGSSVRANDFAGFFAAHPAISHVFFNGAAAEQAFRRHVQ
                     PGIAADGLQLCRLPSTSPAHAGLSRADKLAAWSAILDLPQAAGSV"
     gene            478..>2119
                     /gene="argS"
                     /locus_tag="PLN02_11080"
     CDS             478..>2119
                     /gene="argS"
                     /locus_tag="PLN02_11080"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOI52912.1"
                     /translation="MAQDVKNQLTALLQQALASVAPTATATPIHLERPRDPSHGDFAT
                     NLAMLLAKALKKNPREIANQLLAELPPSALVTAAEVAGAGFINFRLDAGSKTDVVKAV
                     LAERENFGRSTAGGWQKVQVEFVSANPTGPLHVGHGRGAAYGASLSSLLTFAGWDVTR
                     EYYVNDAGRQMDILGLSTWLRYLEQHDVVVPFPPNAYQGGYVRDMATQLKNAHGDKYV
                     RAADAVLAGTPGLPEAGRADDEAKQQRELHLDALIARAKELLGGDWRYVHQHALNEQL
                     EDCRDDLKEFGVEFDVWFSEKALYDTGLVARCVELLEQKGHLYLQNGARWFRSTAFGD
                     EKDRVVQRENGLYTYFASDIAYHLNKFERGFDKVINIWGADHHGYIPRVTGAIKALDL
                     DSDKLQVALVQFAVLYRNGQKASMSTRSGEFVTLRELRGEVGNDACRFFYALRKSDQH
                     LDFDLDLAKSQTNENPVYYIQYAHARVCSVLNQWGGDLASLAEADLSLLANPRELAIA
                     NQLGQFREVIESAARDLAPHMIAFYLKDLAGEFHGWYNA"
BASE COUNT          364 a          788 c          628 g          339 t
ORIGIN      
        1 gcctcgctgc aggccggccg ctactacgcc catccgcgca acgccttctg gccgatcatg
       61 ggcgaactgc tcggtttctc gcccgatctg ccctatgcgg cgcgcctgct tgccctgcag
      121 ggcgccggca tcgccttgtg ggacgtcatc gccggctgcc ggcggcgcgg cagcctggac
      181 gccgacatcg tcggcagcag cgtgcgggcc aacgacttcg ccggtttctt cgccgcccat
      241 ccggcgatca gccatgtctt cttcaacggt gccgccgccg agcaggcctt ccgtcgccat
      301 gtccagcccg gaatcgccgc cgatggcctg caattgtgcc ggctgccgtc gaccagtccg
      361 gcccacgccg ggctttcccg cgccgacaag ctggccgcct ggtcggcaat cctcgacctg
      421 ccgcaggcgg ccggcagcgt ataatcccgc ctttacattt ttttccgcca aagctcaatg
      481 gcccaagatg tcaagaacca gctgaccgcc ctgttgcaac aggccctggc cagcgtcgcc
      541 ccgaccgcca ccgccacgcc gatccacctc gaacgcccgc gcgatccttc gcacggcgat
      601 ttcgccacca acctggcgat gctgctggcc aaggcgctga agaagaatcc gcgcgagatc
      661 gccaaccagc tactcgccga actgccgccg tcggcgctgg tcaccgctgc cgaagtggcc
      721 ggtgccggct tcatcaactt ccgcctcgat gccggaagca agactgacgt ggtcaaggcc
      781 gtgctcgccg aacgggaaaa cttcggccgt tccaccgccg gcggctggca gaaggtgcag
      841 gtcgagttcg tctcggccaa cccgaccggc ccgctgcacg tcggccacgg ccgcggcgcc
      901 gcctacggcg cctcgctgtc cagcctgctg accttcgccg gctgggacgt cacccgcgaa
      961 tactacgtca atgacgccgg ccggcagatg gacatcctcg gcctgtcgac ctggttgcgc
     1021 tacctcgaac aacacgacgt ggtcgtgccc ttcccgccca acgcctacca gggcggctac
     1081 gtgcgcgaca tggcgacgca gttgaagaac gcccatggcg acaagtacgt gcgtgccgcc
     1141 gatgccgttc tcgccggcac gcccggcctg ccggaagccg gtcgcgccga cgacgaagcc
     1201 aagcagcagc gcgagctgca tctcgacgcg ctgatcgccc gcgccaagga actgctcggc
     1261 ggcgactggc gttacgtgca ccagcacgcg ctcaacgaac agctcgagga ctgccgcgac
     1321 gacctcaagg aattcggcgt cgagttcgac gtctggttct cggaaaaggc gctctacgac
     1381 accggcctgg ttgcccgctg cgttgaattg ctcgaacaga aggggcacct ctacctgcag
     1441 aacggcgccc gctggttccg ctcgaccgcc ttcggcgacg agaaggaccg cgtcgttcag
     1501 cgcgaaaacg gtctgtatac ctacttcgcc tccgacatcg cctatcacct gaacaagttc
     1561 gagcgcggtt tcgacaaggt catcaacatc tggggcgccg accaccacgg ctacatcccg
     1621 cgcgtcaccg gcgcgatcaa ggcactcgac ctcgacagcg acaagctgca ggtggcgctg
     1681 gtccagttcg ccgtgctcta ccgcaacggc cagaaggcct cgatgtccac gcgctccggc
     1741 gaattcgtca ccctgcgcga actgcgcggc gaagtcggca acgacgcctg ccgcttcttc
     1801 tacgcgttgc gcaagtccga ccagcacctc gatttcgacc ttgacctggc caagagccag
     1861 accaacgaga acccggtgta ctacatccag tacgcccacg ctcgcgtctg ctcggtgctc
     1921 aaccagtggg gcggcgacct ggccagcctg gccgaggccg acctgtcgct gctggccaac
     1981 ccgcgcgaac tggcgatcgc caaccagctc ggtcagttcc gcgaagtcat cgaaagcgcg
     2041 gcgcgcgacc tggcgccgca catgatcgcc ttctacctca aggacctggc cggcgaattc
     2101 cacggctggt acaacgccg
//
LOCUS       DAOSDX010000527         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18047_length_2118_cov_2.921958, whole genome shotgun sequence.
ACCESSION   DAOSDX010000527 DAOSDX010000000
VERSION     DAOSDX010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18047_length_2118_cov_2.921958"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLN02_11085"
     CDS             complement(<1..447)
                     /locus_tag="PLN02_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOI52913.1"
                     /translation="MNDISTWDIARNLLDGALWTVGLSLTAFVLGGIAGLAVLFARIA
                     KAPLPRRLAKGYIEVFQGTPLLMQLFIIFFGGSLIGLEISPWIAAAIGLTLFTSAYLG
                     EIWRGCVESVPKGQWEASASLAMTHVEQMRHVILPQALRIAIAPTVG"
     gene            complement(444..1112)
                     /locus_tag="PLN02_11090"
     CDS             complement(444..1112)
                     /locus_tag="PLN02_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOI52914.1"
                     /translation="MAYAFDFASVLEYREVLVDGTLKTLALTAIGAVLGLAVGILGAV
                     SRAWKLAPFDRLFAVYVEAIRNTPFLVQLFFIFFGLPSLGVQINEWQAAILAVVVNLG
                     AYTTEIIRAGIEATPKGQIEAAQSLAMSRVQIFRHVVLPPALLKVWPAICSQVVIVML
                     GTSVCSQIAAEELTFAANFIQSRNFRAFETYFVVTAIYFLLAIAVRQVLIQAGQRLIA
                     RRTK"
     gene            complement(1157..1942)
                     /locus_tag="PLN02_11095"
     CDS             complement(1157..1942)
                     /locus_tag="PLN02_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOI52915.1"
                     /translation="MQKRQFLKSLVAASLLAGGIGLAQADVLADAVKRGTLRVAVPQD
                     FPPFGTVGPDLKPRGYDIDVANLIGRELKLKVEIIPVSSTNRIPYLTTGKADLVISSL
                     GKNPDREKVIDFSVAYAPFFNGVFGPADAAVMTAADTAGKTLGVTRGSVEDLEFSKIA
                     PASATIKRFEDNNATISAYLSGQVQLIATGNVVAAAVNERTKIRRLDTKFLIKNSPCY
                     VGVNKGEAAMLAKVNEIIGKLKANGELNQVSQRWLQAPLPAGL"
BASE COUNT          352 a          616 c          800 g          350 t
ORIGIN      
        1 gccgacggtc ggggcgatgg cgatgcgcag cgcctgcggc aggatgacgt ggcgcatctg
       61 ctcgacgtgg gtcatcgcca ggctggccga ggcttcccac tggcctttcg gcaccgattc
      121 gacgcagccg cgccagattt cgccgaggta ggcgctggtg aacaacgtca ggccgatggc
      181 ggcggcgatc cacggcgaga tttcgaggcc gatcaggctg ccgccgaaga agatgatgaa
      241 cagttgcatc agcagcggcg tgccctggaa gacctcgata tagcctttgg ccaaccggcg
      301 cggcagcggg gccttggcga tgcgggcgaa gagcacggcc aggccggcga tgccgccgag
      361 gacgaaggcg gtcagcgaga ggccgacggt ccacaaggcg ccgtcgagca ggttgcgggc
      421 gatgtcccag gtcgagatgt cgttcatttc gttctccggg cgatcagtcg ctggccggcc
      481 tggatcagca cctgacggac ggcgatggcg agcaggaagt agatcgcggt gacgacgaag
      541 taggtttcga aggcgcggaa gttgcgcgac tggatgaagt tggcggcgaa ggtcagttcc
      601 tcggcggcga tctgcgaaca gaccgaggtg ccgagcatga cgatcaccac ctggctgcag
      661 atcgccggcc agaccttgag cagggccggc ggcaggacga cgtggcggaa gatctgcacc
      721 cggctcatcg ccagcgactg cgccgcttcg atctgcccct tgggcgtcgc ttcgatgccg
      781 gcgcggatga tctcggtggt gtaggcgccg agattgacga cgacggcgag gatcgccgcc
      841 tgccattcgt tgatctgcac gccgagcgag ggcaggccga agaagatgaa gaagagctgg
      901 acgaggaagg gggtgttgcg gatcgcctcg acatagaccg cgaacagccg gtcgaagggg
      961 gcgagcttcc aggcccgcga caccgcgccg aggatgccga cggcgagccc gaggacggcg
     1021 ccgatggcgg tcagcgccag cgtcttcagg gtgccgtcga cgagtacctc gcggtattcg
     1081 agcacggaag caaaatcgaa tgcgtaagcc atcggctatc ctccggcgcc gcccgcgggc
     1141 ggcgcccagt gggttatcag agaccggccg gcagcggggc ttgcagccag cgttgcgaaa
     1201 cctggttcag ttcgccgttg gccttgagct tgccgatgat ctcgttgacc ttggccagca
     1261 tcgccgcttc gcccttgttc acgccgacgt agcagggcga gttcttgatc aggaacttgg
     1321 tgtccaggcg gcggatcttg gtgcgctcgt tcaccgccgc ggcgacgaca ttgccggtgg
     1381 cgatcagttg cacctggccg gacaggtagg ccgagatggt cgcgttgttg tcctcgaagc
     1441 gcttgatcgt cgcgctggcc ggggcgatct tgctgaattc caggtcttcg accgagccgc
     1501 gggtgacgcc cagcgtcttg ccggcggtgt cggcggcggt catgacggcg gcgtcggccg
     1561 ggccgaagac accgttgaag aacggcgcgt aggcgaccga gaagtcgatc accttctcgc
     1621 ggtccgggtt cttgcccagg ctggagatga ccaggtcggc cttgccggtg gtcaggtagg
     1681 gaatgcggtt ggtgctgctg accgggatga tctctacctt cagcttgagt tcgcggccga
     1741 tcaggttggc gacgtcgatg tcgtagccgc gcggcttgag gtcggggccg accgtgccga
     1801 agggcgggaa gtcctgcggc acggcgacgc gcagggtacc gcgcttgacg gcatcggcca
     1861 ggacatcggc ctgggcgagg ccgatgccgc cggcgagcag cgaggcggcg acgagggact
     1921 tgaggaattg acgtttctgc atggcgaggc tcctggatgg gtgggaaatc agggttggac
     1981 gaaacggatg ccgacgcggc cggcggcgga aagcaggtgc tgctgcatcg ccttgcgcgc
     2041 gccggcagcg ttgccggcgg cgacggcggc gaggatggcg gcgtgttcgt cggccggctg
     2101 ccagatttgg ctgctgtc
//
LOCUS       DAOSDX010000528         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18095_length_2113_cov_2.953353, whole genome shotgun sequence.
ACCESSION   DAOSDX010000528 DAOSDX010000000
VERSION     DAOSDX010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18095_length_2113_cov_2.953353"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2113)
                     /locus_tag="PLN02_11100"
     CDS             complement(<1..>2113)
                     /locus_tag="PLN02_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOI52916.1"
                     /translation="DTDSYYLDAEAKRDAEALRLITLLPDGAQRVRLVRYTAAQKRAA
                     QLVSGRFAVPAKAEGAQAELDKTLQALAGHFQVQADAAQASRQVAAETRLRAELAPVG
                     EALSLRLVAAPFGADGPRLPVGSGRVRLMAAIGGETLGTERDLAAERKHLESLLDAFP
                     YLEEIGDPAAGEWLIEDPEQALGLVEGLPAQAAIAAIDWPKGKSVRVISVDPGKLGIT
                     VNKERDWFRVAGQAALDEGLVVQLSSLLAAARDKSRFIPIGDGVYAALTRSLKQKLLD
                     LAAVAEVDKQGSKVPQIAAAWLDDVLDGTGLEAGADFRQAIDRLRAAQNILPKLPKSL
                     QAELRPYQEEGYQWAMRLAAAGMGGCLADDMGLGKTLQALAVMLERAKGGATLVVAPT
                     SVCGNWLAEALRFAPSLNARIYSEAGDAEREQLIDQAGPQDVLIVSYTLLQLAQERFA
                     GRSWHTVVADEAQAIKNAATKRSQAVFELPADFRLALSGTPVENRLAELWSIMRFANP
                     GLLGSSGRFNERFAGPIERNRDRDAQHVLKRLIAPFVLRRTKAQVLQDLPPRTELILS
                     VAPESAEAAHYEALRRQAIDEVGTLDAQAGQARFNILAQLTKLRRAACDPRLVSPEFG
                     IVGAKVQAFAELAAELTANGHKTLVFSQFVDFLQILRAPLDAAGISYQYLDGATPAAE
                     RSKRVAAFQAGDSDLFLISLKA"
BASE COUNT          303 a          714 c          752 g          344 t
ORIGIN      
        1 ggctttcagg ctgatcagga agaggtcgct gtcgccggcc tggaaggcgg cgacgcgctt
       61 gctgcgttcg gcggccggcg tcgcgccgtc caggtactgg tagctgatgc cggcggcgtc
      121 gagcggcgcg cgcaggatct gcaggaaatc gacgaactgg ctgaagacca gggtcttgtg
      181 gccgttggcg gtgagttcgg cggccagttc ggcgaaggcc tgcaccttgg cgccgacgat
      241 gccgaattcc gggctgacca ggcgcggatc gcaggcggcg cggcgcagct tggtcagttg
      301 cgccaggatg ttgaaacgcg cctgcccggc ctgggcatcg agcgtcccga cctcgtcgat
      361 cgcctgccgg cgcagcgctt cgtagtgcgc cgcctcggcg gactccggag cgaccgagag
      421 gatcaactcg gtacgcggcg gcagatcctg cagcacctgc gccttggtgc ggcgcaggac
      481 gaagggggcg atcaagcgtt tcaggacgtg ctgggcgtcg cggtcgcggt tgcgttcgat
      541 ggggccggcg aagcgttcgt tgaagcggcc gctgctgccg agcaggccgg ggttggcgaa
      601 gcgcatgatc gaccacagtt cggccagccg gttttccacc ggcgttcccg agagggccag
      661 gcgaaaatcg gccggcagtt cgaagaccgc ctgcgaacgc ttggtcgccg cgttcttgat
      721 cgcctgcgcc tcgtcggcga cgacggtgtg ccatgagcgg ccggcaaagc gttcctgggc
      781 cagttgcagc agggtgtagg agacgatcag cacatcctgg ggaccggcct ggtcgatcaa
      841 ctgttcgcgt tcggcgtcgc cggcctcgct gtagatgcgg gcgttcaggc tgggcgcgaa
      901 acgcagcgcc tcggccagcc agttgccgca caccgaagtc ggcgcgacga ccagggtggc
      961 gccgcctttc gcccgttcga gcatgacggc gagcgcctgc agggtcttgc ccagccccat
     1021 gtcgtcggcc aggcagccgc ccatgccggc ggcggcgagg cgcatcgccc actggtagcc
     1081 ctcctcctgg taagggcgca gctcggcctg cagggatttc ggcagcttcg gcaggatgtt
     1141 ctgcgcggcg cgcaggcggt cgatggcctg gcggaaatcg gccccggcct ccagcccggt
     1201 gccgtcgagc acgtcgtcga gccaggcggc ggcgatctgc ggcaccttgc tgccctgctt
     1261 gtcgacttcg gcgaccgccg ccagatcgag cagtttctgt ttcagcgagc gggtcaacgc
     1321 ggcatagacg ccgtcgccga tcggaatgaa gcggctcttg tcgcgggcgg cggcaagcag
     1381 gctggagagt tgcacgacga ggccttcatc aagcgccgcc tgaccggcga cgcggaacca
     1441 gtcgcgctcc ttgttgacgg tgatgccgag cttgccggga tcgacgctga tgacgcggac
     1501 gctcttgccc ttcggccagt cgatggcggc gatggcggct tgtgccggca agccctcgac
     1561 cagcccgagc gcctgctccg ggtcttcgat cagccactcg ccggccgccg gatcgccgat
     1621 ctcttcaagg taagggaaag cgtcgagcag actttccaga tgtttccttt ccgccgccag
     1681 atcgcgctcg gtacccaggg tttcgccgcc gatcgccgcc atcagccgga cccgaccgct
     1741 acccaccggc aaacgcgggc cgtcggcgcc gaagggggcg gcgaccaggc gcagcgacag
     1801 cgcttcgccg accggcgcca gttccgcccg caggcgcgtc tccgccgcta cctgccgcga
     1861 agcctgcgcc gcatcggcct gcacctggaa atgcccggcc agcgcctgca aggtcttgtc
     1921 gagttcggcc tgcgccccct cggccttcgc cggcaccgcg aaacgcccgg acaccagttg
     1981 cgcggcgcgt ttttgtgccg ccgtgtagcg caccagccgg acgcgctgcg cgccgtccgg
     2041 caacaaggtg atcaggcgca gcgcctcggc gtcgcgtttg gcttccgcat ccaggtaata
     2101 gctgtcggta tcc
//
LOCUS       DAOSDX010000529         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18096_length_2113_cov_2.492711, whole genome shotgun sequence.
ACCESSION   DAOSDX010000529 DAOSDX010000000
VERSION     DAOSDX010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18096_length_2113_cov_2.492711"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLN02_11105"
     CDS             complement(<1..606)
                     /locus_tag="PLN02_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI52917.1"
                     /translation="MNRRQLIRHLATLALCERFSFASRAWAAGQALPPPGVHEFSGEV
                     TVNGQPARPRMTIGAGDRIVTGNNAQAIYIIGSDAYLQRADSTVQVIEDNATRRVLRI
                     LQGKLMGVFGKGEKSLQTPTATIGIRGTACYIEATAAQVYFCLCYGEAEIRAPGETTI
                     LETIATTYHDHPLYLNADRQRMMVPARVINHTDAELILLESL"
     gene            838..>2113
                     /locus_tag="PLN02_11110"
     CDS             838..>2113
                     /locus_tag="PLN02_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI52918.1"
                     /translation="MAVIGAGVGGLSTAIRLAQLGLAVTVFERLDRVGGLCGTVVFKG
                     RRYVVGCNDFGHGLLRDLKALGITLPFEHKKTRVHFAGEHYSMPPGPQTILNFARHLP
                     SFVRYVMGLRKARRRAYDKVRFLDQLIDEARVGGRFADILMLPAYLMGVPPDRFRVDS
                     ILDEFTFHYGYANPITPVGGPQALADALHQRLLALGGQVVLGCEVTGVETMAGKKRVH
                     TSQGGHDVDLLVSTLLKPGHFPPTCESGLPLSMLWLDIDPAYRLPRRIHTHLFYPPGI
                     RQWFGDIYAGRMPAEFGFHFFCSDLGVQDGVNTASVYCHLPRGLTEDAAIQTAAQAYI
                     LERIESLLPGIGAHLRACTMISPALFREMHGMDPVLTPVIAPAGFPQPENHAPEDDVY
                     YAGAAAFPPGMHSGAAIRTSAYVSELVARRLTD"
BASE COUNT          327 a          643 c          721 g          422 t
ORIGIN      
        1 caggctttcc agcaggatca gttcggcgtc ggtgtggttg atcacgcggg ccggcaccat
       61 catccgctgc cggtcggcgt tcaggtagag cgggtgatcg tggtaggtgg tggcgatcgt
      121 ctcgaggatt gtcgtctcgc ccggggcgcg aatctcggct tcgccatagc acaggcagaa
      181 atagacctgc gccgcggtcg cctcgatgta gcaggcggta ccgcggatgc cgatggtggc
      241 ggtcggcgtc tgcaggctct tttcgccttt gccgaaaacg cccatcagtt tgccttgcag
      301 gatgcgcagg acgcgccggg tggcgttgtc ctcgatgacc tggacggtgg aatccgcccg
      361 ctgcaggtag gcgtcactgc cgatgatgta gatcgcctgg gcattgttgc cggtgacgat
      421 gcggtcgccg gcgccgatcg tcatgcgcgg gcgggccggc tggccgttga ccgtgacttc
      481 gccgctgaac tcgtgcacac cgggcggcgg cagcgcctgg ccggcggccc aggcgcggct
      541 ggcgaaggaa aagcgttcgc acagcgcgag cgtggcgaga tggcggatca attggcgacg
      601 attcatcggt ggtcttctcc gttggcttgg gtgggctgtg tatgctgcgc tgcttcggca
      661 agcgtaacca atcggcggcg caaatgctcg gcgagaacaa aaagaattcg ttgcgggcca
      721 gggcgctcgg tcggccttgc gcggatcgcc gcggcaaagg aagatggtcg ctgtcagctt
      781 ggcaaaaggt gcagattgat gacgaaacgg aattccggaa ttgcgcagcg tcccgaggtg
      841 gcggtcatcg gagccggtgt cggcggattg agtacggcca tccgccttgc ccaacttggt
      901 ctggcggtga ccgtgttcga gcgtctcgac cgggtcggcg gcttgtgtgg caccgtcgtt
      961 ttcaaggggc ggcgctatgt cgtcggctgc aacgattttg gccatggtct gctgcgcgat
     1021 ctgaaggcgc tcggcatcac cttgcccttc gagcacaaga agacccgtgt ccatttcgcc
     1081 ggcgagcatt acagcatgcc gcccggcccg cagacgatcc tgaattttgc ccgccacctg
     1141 ccgtcgttcg tccgttacgt catgggcttg cgcaaggcgc ggcggcgcgc ttacgacaag
     1201 gtccgtttcc ttgaccaact gatcgacgaa gcgcgcgtcg gcgggcgctt tgccgacatc
     1261 ctgatgctgc cggcctacct gatgggggtg ccgccggacc gtttccgcgt cgattcgatc
     1321 ctcgacgaat tcactttcca ttacggctac gccaacccga tcacgccggt cggcggtccg
     1381 caggcgctgg ccgatgccct gcaccagcgc ttgctggcgc tcggcggcca ggtcgttctc
     1441 ggttgcgagg tgacgggcgt cgagaccatg gcgggaaaga agcgagttca taccagtcag
     1501 ggcggccacg acgtcgatct gctggtgtcg acgctgctga agcctggcca tttcccaccc
     1561 acttgcgaat ccgggctgcc gctgtcgatg ctgtggctgg acatcgatcc ggcctaccgc
     1621 ctgccgcgca gaatccatac gcatttgttc tatccaccgg gaatccgcca gtggttcggc
     1681 gacatctacg cgggccggat gccggccgaa ttcggcttcc acttcttctg cagcgatctt
     1741 ggcgtccagg acggggtcaa tacggccagc gtctattgcc atctgccgcg cgggctgacc
     1801 gaggatgccg cgatccagac ggccgcgcag gcttacattc tggaacggat cgaaagcctg
     1861 ctgccgggca tcggcgctca tctgcgtgct tgcacgatga tttcgccggc gctgttccgg
     1921 gagatgcacg ggatggaccc ggtactgacg ccggtcattg cgccagcggg ttttccgcag
     1981 ccggaaaacc acgcgccgga ggacgacgtg tattacgccg gtgccgctgc attcccgccg
     2041 ggcatgcatt cgggggcggc gatccgtacg tcggcttacg tcagcgagtt ggttgcccgg
     2101 cgcttgacgg ata
//
LOCUS       DAOSDX010000530         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18112_length_2111_cov_3.483463, whole genome shotgun sequence.
ACCESSION   DAOSDX010000530 DAOSDX010000000
VERSION     DAOSDX010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18112_length_2111_cov_3.483463"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..968)
                     /locus_tag="PLN02_11115"
     CDS             complement(60..968)
                     /locus_tag="PLN02_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOI52919.1"
                     /translation="MGLLRLLALTLCALLAACDRPQDAALPLDYRTTPAAETTPLYRF
                     AVHPLHNPNKLAEAYQPLIDLLNASIPEARFELEASRDYQAYEAKYRARGPEFLLPNP
                     WQSLQAMKLGYRVIAMAGDAEDFRGLFIVRRDSPIRHPADLKGKRVGYPSPTALAACI
                     MPQYFLHENGIDINHDIENSYVGSQESAIMSAYLGHTAVAATWPPPWRLFLRDHPAEA
                     AQLRVLWETEPLLNNSVMVRNNVPEPIVQAVRNILLKLEDSDSGQLVLAAMATARFHF
                     ADDTTYQVVDAYIRRFEQEVRPVEQR"
     gene            complement(1050..1853)
                     /locus_tag="PLN02_11120"
     CDS             complement(1050..1853)
                     /locus_tag="PLN02_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOI52920.1"
                     /translation="MDAIRRYDAGFPSENLPMDNRNSWPLLGLGLLLALGLIGGATVL
                     AKHFKTLGAPKQSISVKGLAEKPVKADSAEWSIGISVHGDDFAATLNALRQARPIIDG
                     FLAEQGFAADSASAGPESVSPHHEYEPLPQGGSRQVQRGFDGKQLFVVRSTALDTIAK
                     AHSAIIELKAQGKPIVYDDPRYLINDLESVKMSLIGAATRNARQRAEEFAKVGDARVG
                     NMRSASQGAFYILPANGTEEDASDYGGVYDKTTVDKKARVVVTIEYAIE"
     gene            complement(1873..>2111)
                     /locus_tag="PLN02_11125"
     CDS             complement(1873..>2111)
                     /locus_tag="PLN02_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOI52921.1"
                     /translation="QMAWTLIKKHALTAGIPRERISPHVLRHAFATHLLNHGADLRVV
                     QLLLGHADISTTQIYTHVARERLKALHAIHHPRG"
BASE COUNT          336 a          624 c          760 g          391 t
ORIGIN      
        1 ggcgacgccg accgccagtt ggcgccgcaa gttgccgagc agccgatggc gaagctgctt
       61 catcgctgtt ccaccggacg cacttcctgc tcgaaacgcc ggatgtaggc atcaaccacc
      121 tgataagtcg tgtcatccgc gaaatggaag cgggcggtgg ccattgccgc caggaccagt
      181 tggccgctgt cgctgtcctc cagcttgagc aggatgttac gtaccgcctg gacgatgggc
      241 tcggggacat tgttacgcac catgaccgaa ttgttgagca gcggttcggt ttcccaaagc
      301 acgcgcagtt gcgcggcctc ggccggatgg tcgcgcagga acaggcgcca gggcggcggc
      361 caggtggcgg cgaccgcggt atggccgaga taggcgctca tgatcgccga ttcctgcgag
      421 ccgacgtagc tgttttcgat gtcatgattg atgtcgatgc cgttctcgtg caggaagtac
      481 tgcggcatga tgcaggcggc gagcgcggtc ggcgacggat agccgacgcg cttgcccttg
      541 aggtccgccg gatggcggat cgggctgtcg cgacggacga tgaagaggcc gcgaaaatcc
      601 tcggcgtcgc cggccatggc gatgacgcgg tagcccagct tcatcgcctg cagcgactgc
      661 caggggttgg ggagcaggaa ttccgggccg cgggcgcggt atttcgcctc gtaggcctgg
      721 tagtcacgcg atgcctcgag ttcgaagcgg gcttccggaa tgctggcgtt gagcaggtcg
      781 atcagcggct ggtaggcttc ggcgagcttg ttcgggttat gcagcggatg caccgcaaag
      841 cggtagagcg gggtggtttc ggccgctggc gtcgtccggt agtccagcgg cagggcggcg
      901 tcttgcgggc gatcgcaggc ggcgagcagc gcgcacagcg tcagtgccag caggcgaagc
      961 aggcccatcg atctccctcc gtgtttttct gccagtcaag gtagcgccaa acgtcggacc
     1021 cggcaagccc ggcaaaaccc cggtgctgcc tactcgatgg cgtattcgat ggtgacgacg
     1081 acgcgcgcct tcttgtccac cgtcgtcttg tcatagaccc cgccgtaatc gctggcgtcc
     1141 tcttcggtgc cgttggccgg caggatgtag aaagcgccct gcgacgccga gcgcatgttg
     1201 ccgacccggg cgtcgccgac cttggcgaat tcttcggcgc gttgccgggc gttgcgggtt
     1261 gccgcgccga tcaaggacat cttgacgctt tccaggtcgt tgatcaggta gcgcggatcg
     1321 tcatagacga tgggcttgcc ctgcgccttg agttcgatga tcgcgctgtg cgccttggcg
     1381 atggtatcga gcgcggtgga acggacgacg aacagttgct tgccgtcgaa gccgcgctgc
     1441 acctggcgcg agccgccttg cggcaggggt tcgtactcgt ggtgtggact gaccgactcc
     1501 gggccggcgc tggcgctgtc ggcggcgaag ccctgctcgg ccaggaaacc gtcgatgatc
     1561 ggccgcgcct ggcgcagcgc gttcagggtg gcggcgaaat cgtcgccgtg cacggaaatg
     1621 ccgatgctcc attcggcgga gtcggccttg accggtttct cggccagccc cttgaccgaa
     1681 atgctctgct tgggcgcacc cagcgtcttg aaatgcttcg ccagcaccgt cgcgccgccg
     1741 atcaggccaa gggcgaggag gaggccgagg ccgagcagcg gccaggaatt gcggttgtcc
     1801 atggggaggt tctcggaagg aaagccggca tcgtaacggc ggatcgcgtc catgccaatg
     1861 gcaaccgtgt tttcagccgc gcggatggtg gatggcgtgc agcgctttca gacgttcgcg
     1921 ggcgacatgg gtgtagatct gcgtcgtcga gatgtcggca tggccaagca gcaactggac
     1981 gacccgcagg tcggcgccgt ggttgagcag gtgggtggcg aaggcgtggc gcagcacgtg
     2041 gggcgagatg cgttcgcgcg ggatgccggc ggtcagcgcg tgcttcttga tcagcgtcca
     2101 ggccatctgg c
//
LOCUS       DAOSDX010000531         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18131_length_2109_cov_3.623661, whole genome shotgun sequence.
ACCESSION   DAOSDX010000531 DAOSDX010000000
VERSION     DAOSDX010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18131_length_2109_cov_3.623661"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLN02_11130"
     CDS             complement(<1..754)
                     /locus_tag="PLN02_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52922.1"
                     /translation="MKRSWLCGLGLLAASATPPALAHHGVAGVGAVGLQGPGAPIESA
                     ASSLLPEGGVLAYLKLDQARFKQYNWAAPNADYARFTMLGLGYGVTPWFSAYAFVPHN
                     TKVEESGGLDSRGWADMSLMGQVGFKYDQDGWRLTPASESLDDLEDWHFSVFAGATLP
                     TGAPNHRLSDGSIDPGKSLGFGKPAWTLGVTASKMLSQTLTLSLEASTFRFQPYTYDS
                     GDRVQFGTENRLNAALAWRALTVPEKSFRLDPV"
     gene            complement(751..1176)
                     /locus_tag="PLN02_11135"
     CDS             complement(751..1176)
                     /locus_tag="PLN02_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52923.1"
                     /translation="MKWLPLLALFAAATASAHEIRLDVSGSSATVITLHYADGQPFAF
                     EAYELYPAGSETPGQVGRTDGHGRIALLADSAGEWRLKAWSDDGHGVDRKLTALAGDP
                     AAATAPVAETPRAALWLGGLGTIFGLFGLAQLYLGRKKR"
     gene            complement(1173..1865)
                     /locus_tag="PLN02_11140"
     CDS             complement(1173..1865)
                     /locus_tag="PLN02_11140"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HOI52924.1"
                     /translation="MRRTLSLLLCLTSSAAFAHDLWFAPEGNDYLLLQGHRHSGHAGA
                     DRIDYDGRKVTGSLCLDGAGKPRPATPSPSAPVRFAGPCAVLQADYVSGYWTKTAWET
                     KNVPKTGIAGVLRSWHSAESVKRIGQWIPAAGKPLGRGLEITPLRDPLPLQPGDKLVV
                     LVSDDGKPVTGVPVAYGDDTRGVSDGDGKIAIRLRHGGTQLITTTLEMPLNDGKADRS
                     LRSATLQFEIAP"
BASE COUNT          326 a          660 c          763 g          360 t
ORIGIN      
        1 gcaccgggtc gagacggaag gatttctccg gcacggtcag cgcccgccag gcgagcgccg
       61 cgttgaggcg gttctcggtg ccgaactgca cgcggtcgcc gctatcgtag gtgtagggct
      121 ggaagcggaa ggtcgatgct tccaggctca gggtcagcgt ctggctgagc atcttgctgg
      181 cggtgacgcc gagcgtccac gccggcttgc cgaaaccgag cgacttgcca gggtcgatcg
      241 aaccgtcgct caggcggtgg ttgggcgcgc cggtgggcag cgtggcgccg gcaaagacgc
      301 tgaaatgcca gtcctcgagg tcgtcaaggc tctcgctggc cggggtcagg cgccagccgt
      361 cctggtcgta tttgaagccg acctggccca tcagcgacat gtcggcccag ccgcgcgaat
      421 cgagaccgcc ggattcctcg accttggtgt tgtgcggcac gaaggcgtag gcggagaacc
      481 agggggtgac gccgtaaccg aggccgagca tggtgaaacg ggcgtagtcg gcgttcggcg
      541 ccgcccagtt gtattgcttg aagcgcgcct gatcgagctt caggtaggca agcacgccgc
      601 cttccggcaa cagtgaggag gccgccgatt cgatcggtgc ccccggcccc tgcaggccaa
      661 cggcgccgac gccggcgacg ccgtgatggg cgagcgccgg tggcgtcgcg cttgccgcca
      721 gcaagccgag gccgcaaagc caggatcgtt tcatcgtttc ttcctcccca gatagagttg
      781 cgccaggccg aacaggccga aaatcgttcc caggccgccc agccagagcg cggcgcgcgg
      841 tgtttcggcg acgggcgccg tggctgccgc cgggtcgccg gccagtgccg tgagcttgcg
      901 gtcgacgccg tggccgtcgt ccgaccaggc cttcagccgc cactcgccgg cgctgtcggc
      961 gagcagcgcg atgcggccgt ggccgtcggt gcggccgact tggcccggcg tctcgctgcc
     1021 ggccgggtag agttcgtagg cctcgaaggc gaagggctgg ccgtcggcgt aatgcagcgt
     1081 gatcaccgtc gcgctcgacc cgctaacgtc gagccggatt tcgtgagcgc ttgccgtcgc
     1141 ggcggcgaac aacgcgagta agggcagcca cttcatggcg cgatctcgaa ctgcagcgtc
     1201 gcgctgcgta gcgaacggtc ggccttgccg tcgttgagtg gcatctccag cgtcgtcgtg
     1261 atcaactggg tgccgccgtg gcgcaggcgg atggcgattt tgccgtcgcc gtcgctgacg
     1321 ccgcgggtgt cgtcgccgta ggccaccggc acgccggtga ccggtttgcc atcgtcgctg
     1381 accagaacaa cgagcttgtc acccggctgc agtggcagcg gatcgcgcag cggcgtgatc
     1441 tccaggccgc ggccgagcgg cttgccggcg gcgggaatcc actggccgat gcgcttcacc
     1501 gattcggccg aatgccaact gcgcagcacg ccagcgatcc cggttttcgg gacgttcttc
     1561 gtttcccagg cggtcttggt ccagtagccg gaaacgtaat ccgcctgcag caccgcgcag
     1621 ggaccggcga aacgcacggg ggcgctgggc gatggcgtgg ccgggcgggg tttgcctgcg
     1681 ccatcgaggc acagcgagcc cgtcaccttg cggccatcgt agtcgatgcg gtcggcgccg
     1741 gcgtggccgg aatggcggtg accctgcagc agcaggtaat cgttgccttc gggggcgaac
     1801 cacaggtcgt gggcaaaggc ggccgaagat gtcaggcaga gcagcaggga gagcgtgcgg
     1861 cgcatgatgg gtggcgtgta tgaaagaagt taatatcttc atacaagttc cgtgcccagg
     1921 caataagtca ttgaacgtat tgtctgcggc agcaatggca gcgtccctgg ctggccgaaa
     1981 tggaatgtcg ctttccggcg tggtggaaag cgagcattcg ctgttgcgcc gagcgctttt
     2041 cccgctgacc tggcgggcgc cgccgggttt cccgaactgg tttgcatctt gcttgttcag
     2101 agcggcggc
//
LOCUS       DAOSDX010000532         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18164_length_2106_cov_3.580692, whole genome shotgun sequence.
ACCESSION   DAOSDX010000532 DAOSDX010000000
VERSION     DAOSDX010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18164_length_2106_cov_3.580692"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="ccoG"
                     /locus_tag="PLN02_11145"
     CDS             complement(<1..854)
                     /gene="ccoG"
                     /locus_tag="PLN02_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOI52925.1"
                     /translation="MAHPTPRKIIDIRPAGDPEAKVYPRSVDGPFTRWRWFFVWLTQA
                     VFYGTCWLPWDGRQAVLFDLDARKFHLFGLVLWPQDTIYLVILMIVGALALFLFTAVA
                     GRLWCGYSCPQTVYTEIFLWLEKLIEGDRARRIKLDAQPWSARKLAIKGGKHLAWGLF
                     ALWTGITFVGYFVPIRELVGQLASFSAGPWASFWVVFYAFATYGNAGFLREQMCRQIC
                     PYARFQFVMLDPDTLIIAYDSQRGDARGARPKGADPKVLGLGDCVDCGICVQVCPTGI
                     DIRDGLQN"
     gene            978..1427
                     /locus_tag="PLN02_11150"
     CDS             978..1427
                     /locus_tag="PLN02_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOI52926.1"
                     /translation="MRLSTFSDYSLRVLLYLGERPSGLISIGEIAAWHEISESHLMKV
                     VLHLGRCGYVQTVRGKGGGLRLAHPPAAIGLGEVLRQTETDFAMADCFGTTYPCRIDG
                     ICRIKGVLREALQAMFLVLDSYTLADLLADPDALPLATPAREDARHA"
     gene            1420..1635
                     /locus_tag="PLN02_11155"
     CDS             1420..1635
                     /locus_tag="PLN02_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52927.1"
                     /translation="MREFSPPVGEELAIDADHEPELSDADILDAMQQLPGYIDIGTED
                     FRLIYHLAWRHAVERLRRRQAGRDDAA"
     gene            1632..>2106
                     /locus_tag="PLN02_11160"
     CDS             1632..>2106
                     /locus_tag="PLN02_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HOI52928.1"
                     /translation="MSPPENRLRAYFRKWRGSTAGSPPRVNNSEILWSWLGAFAGIGL
                     LAWLGQYSFSAYSLHLLIGSFGASAVLLFGAPRSPLAQPRNLLGGHALSAAVGVVCWQ
                     LLHGQPVLAQALAVASAIALMHLTRCLHPPGGATALIATLGSTEIERLGYWYILWP"
BASE COUNT          366 a          766 c          648 g          326 t
ORIGIN      
        1 tcgttctgca ggccgtcgcg gatgtcgatg ccggtcgggc agacctgcac gcagatgccg
       61 caatcgacgc agtcgcccag cccgagcacc ttgggatcgg cccccttggg gcgggcgccg
      121 cgggcgtcgc cgcgctggct gtcgtaagcg atgatcagcg tgtccgggtc gagcatgacg
      181 aactggaagc gggcatacgg acagatctgc cggcacatct gttcgcgcag gaaaccggcg
      241 ttgccgtagg tggcgaaggc gtagaagacg acccagaagc tcgcccacgg cccggcggaa
      301 aagcttgcca gctggccgac cagctcgcgg atcggcacga agtagccgac gaaggtgatg
      361 ccggtccaca atgcgaacag cccccaggcc aggtgcttgc cgcccttgat cgccagcttg
      421 cgggcgctcc acggctgggc gtcgagcttg atgcggcggg cacggtcgcc ctcgatcagc
      481 ttctccagcc agaggaagat ttccgtgtag accgtctgcg ggcaactgta gccgcaccac
      541 aggcgaccgg cgacggcggt gaacaggaac agcgccagcg cgccgacgat catcaggatc
      601 accaggtaga tcgtgtcctg cggccacagc accaggccga agaggtggaa cttgcgggcg
      661 tcgagatcga acagcaccgc ctgccgaccg tcccagggca gccagcaggt accgtagaaa
      721 accgcctgcg tcagccagac gaagaaccag cgccagcggg tgaacggccc gtccaccgaa
      781 cgcgggtaga ccttggcctc gggatcgccg gcgggacgga tgtcgatgat ttttctcggg
      841 gtggggtggg ccatttctgc gctgcctgaa aactccccgc cagacgagga aatcataagt
      901 ggaataataa attccgcttt atggtagcga ctcacctcct tataatcaac attcaaaatt
      961 ccccttatgg aaccggcatg cgactgagca ccttttccga ctacagcctg cgcgtcctgc
     1021 tctacctggg cgagcgcccg agcggactga tcagcatcgg cgaaatcgcc gcctggcacg
     1081 agatttcgga aagccacctg atgaaggtcg tcctccacct cggccgctgc ggctacgtgc
     1141 agaccgtgcg cggcaagggc ggcggcctgc gcctggcgca cccgccggcc gcaatcggcc
     1201 tgggcgaggt gctgcgccag accgaaaccg acttcgcgat ggccgactgt ttcggcacca
     1261 cctacccctg ccgcatcgac ggcatctgcc gaatcaaggg cgtgctgcgc gaagccttgc
     1321 aggcgatgtt tctggtcctc gacagctaca cgcttgccga cttgctggcc gatcctgacg
     1381 ccctgcccct tgccacacca gcccgcgagg acgcccgcca tgcgtgaatt ctcgccgccg
     1441 gtcggcgagg aactcgccat cgacgccgac cacgaaccgg agttgtcgga tgccgacatc
     1501 ctcgacgcca tgcagcaact gcccggctac atcgacatcg gcaccgaaga cttccgcctg
     1561 atctaccacc tcgcctggcg ccacgccgtc gaacgcctgc gccgccggca ggcgggccgg
     1621 gacgacgccg cgtgagcccg ccggaaaacc ggctgcgcgc ctacttccgc aagtggcgcg
     1681 gcagcaccgc cggcagcccg ccgcgcgtca acaacagcga aatcctctgg tcctggctcg
     1741 gcgccttcgc cggtatcggc ctgctcgcct ggctcggcca gtacagcttc agcgcctaca
     1801 gcctgcacct gctgatcggc tccttcggcg cctccgccgt cctcctcttc ggcgccccgc
     1861 gcagcccact cgcccagccg cgcaacctgc tcggcggcca cgccctctcc gccgccgtcg
     1921 gcgtcgtctg ctggcaactg ctgcacggcc agccggtgct cgcccaggcc ctcgccgtcg
     1981 ccagcgccat cgccctgatg cacctcaccc gctgcctgca ccctcccggc ggcgccaccg
     2041 ccttgatcgc cactctgggc agcaccgaga tcgaacgcct cggctactgg tacatcctct
     2101 ggcccg
//
LOCUS       DAOSDX010000533         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18175_length_2104_cov_3.186920, whole genome shotgun sequence.
ACCESSION   DAOSDX010000533 DAOSDX010000000
VERSION     DAOSDX010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18175_length_2104_cov_3.186920"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLN02_11165"
     CDS             <1..270
                     /locus_tag="PLN02_11165"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOI52929.1"
                     /translation="PVVYMMDRYVVGGFYRVHTGRGKDENLNAPGMHFEPLAFETGCN
                     LPDYRCGNPDSPPNRFYAYGVVARLACLAAAVELERTAPPVDLPA"
     gene            276..1295
                     /gene="gshB"
                     /locus_tag="PLN02_11170"
     CDS             276..1295
                     /gene="gshB"
                     /locus_tag="PLN02_11170"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HOI52930.1"
                     /translation="MTQQLHFERHLLGGSERPLRLVFIIDPLDRLKAWKDSSVALMRA
                     AQSHGHDVFAIEAPTLGWRKPEADHPGGSFGEAIHLHLRPDDHDWYRETQREWLPLKA
                     FDAVLMRKDPPVDGEYLAACSLLERAEADGVRVFNRPRALRDHAAKLAILEFPQFIPS
                     TKVSRDLGRLQQFIDEQRDVIVKPLDSNSGSRIFRLHRNDPNRNVILETLTGNGRHTV
                     MAQQYLQAIAQGDKRILLIAGKAVPWCLARIPKAGETRGNLSAGGTGVVQELTPGERH
                     IAEALGPLLLKRGLLLVGLDLIGERLTGINVTSPTGMVEIRQQSGFDAAGATITAIEH
                     ACAVS"
     gene            1280..>2104
                     /locus_tag="PLN02_11175"
     CDS             1280..>2104
                     /locus_tag="PLN02_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOI52931.1"
                     /translation="MRRLLILLVATLVAACGKAPLQEQQAYVFGTRVEVVVVGENPDL
                     GRQAIAAVLREFDRMHRTYHAWQPSELTAVNEAIAAGRKATVTPEMAAFMGEAQALSA
                     QGDHLFDPGIGALIKLWGFHADEFTARLPEPQALAGWLKEKPSIADLVIDGTTVSSRK
                     RALALDFGGYLKGVALDRAAAILRSQGINNALINIGGNVMALGSKEGRKWRVGIQHPR
                     QPGPLATVQLDDGEAIGTSGDYQRYFELDGRRYSHLIDPRSATPVEHTQAVTVLIPA"
BASE COUNT          357 a          805 c          650 g          292 t
ORIGIN      
        1 ccggtggtgt acatgatgga tcgctatgtc gtcggcggct tctaccgcgt gcacaccggt
       61 cgcggcaagg acgagaacct caacgcgccg ggcatgcatt tcgagccgct ggccttcgaa
      121 accggctgca acctgcccga ctaccgctgc ggcaaccccg actcgccgcc caaccgcttc
      181 tacgcctacg gcgtcgtcgc ccgcctggcc tgtctcgccg ctgccgtcga actcgaacgg
      241 acggcaccgc cggtcgacct cccggcctga cgccgatgac ccagcagctg cacttcgaac
      301 ggcatctgct tggcggcagc gaaaggccgc tgcgactggt cttcatcatc gatccgctgg
      361 atcgcctgaa ggcctggaag gactcctcgg tggcgctgat gcgggcggcg cagagccatg
      421 gtcacgacgt cttcgccatc gaggcgccga cgctcggctg gcgcaagccg gaggccgacc
      481 atccgggcgg cagcttcggc gaggcgatcc acctccatct gcgccccgac gaccacgact
      541 ggtatcgcga gacgcagcgc gaatggctgc cgctgaaggc cttcgacgcc gtgctgatgc
      601 gcaaggaccc gccggtcgat ggcgaatacc tcgccgcctg cagcctgctc gaacgcgccg
      661 aggccgacgg cgtgcgcgtc ttcaaccggc cgcgggcgct gcgcgaccat gcggccaagc
      721 tggcgatcct cgaattcccg cagttcatcc cgtccaccaa ggtcagccgc gacctcggcc
      781 gcctgcagca attcatcgac gaacagcgcg acgtgatcgt caagccgctc gacagcaaca
      841 gcggcagccg catcttccgc ctgcaccgca acgaccccaa ccgcaacgtc atcctcgaga
      901 cgctgaccgg caacggccgc cataccgtga tggcgcagca gtacctgcaa gccatcgccc
      961 agggcgacaa gcgcatcctg ctcatcgccg gcaaggcggt gccctggtgc ctggcgcgca
     1021 ttcccaaagc cggcgagacg cgcggcaacc tgagcgccgg cggcaccggc gtcgtccagg
     1081 aactcacccc cggcgaacgc cacatcgccg aagccctcgg cccgctgctg ctcaagcgcg
     1141 gcctgctgct ggtcggtctc gacctgatcg gcgagcggct caccgggatc aacgtcacca
     1201 gccccaccgg catggtcgaa atccgccagc agagcggctt cgacgccgcc ggtgcgacca
     1261 tcaccgcaat cgaacacgca tgcgccgtct cctgatcctc cttgtcgcca ccctcgtcgc
     1321 cgcctgcggc aaggccccgc tgcaggagca gcaggcctac gtcttcggga cccgggtcga
     1381 ggtcgtcgtc gtcggcgaaa acccggatct cggccgccag gcgatcgccg ccgtgctgcg
     1441 cgaattcgac cgcatgcacc ggacctacca cgcctggcag ccgtcggaac tgaccgccgt
     1501 gaacgaagcc atcgccgccg gccggaaggc cacggtgacg ccggaaatgg ccgctttcat
     1561 gggcgaggcg caggcgctct cggcgcaggg cgaccacctg ttcgaccccg gcatcggcgc
     1621 cttgatcaag ctctggggct tccacgccga cgaattcacg gcccgcctgc ccgagccgca
     1681 ggcgctcgcc ggctggctca aggagaaacc gtcgatcgcc gatctggtca tcgacgggac
     1741 cacggtttcc agccgcaagc gcgccctggc cctcgatttc ggcggctacc tgaagggcgt
     1801 cgcgctcgac cgcgccgccg ccatcctgcg cagccagggg atcaacaacg cgctgatcaa
     1861 catcggcggc aacgtcatgg cgctcggcag caaggaaggc cgcaagtggc gggtcggcat
     1921 ccagcacccg cgccaacccg gcccgctggc caccgtacag ctcgacgacg gcgaggcgat
     1981 cggcacttcc ggcgactacc agcgctactt cgaactcgac ggccgccgct actcgcacct
     2041 gatcgatccg cgcagcgcca ccccggtcga gcacacgcag gcggtcaccg tcctcatccc
     2101 ggct
//
LOCUS       DAOSDX010000534         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18202_length_2101_cov_2.374878, whole genome shotgun sequence.
ACCESSION   DAOSDX010000534 DAOSDX010000000
VERSION     DAOSDX010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18202_length_2101_cov_2.374878"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLN02_11180"
     CDS             <1..691
                     /locus_tag="PLN02_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI52932.1"
                     /translation="GAEALIRWRHPERGLVPPDRFIPLIEQSGLIDGLGLWVLDDACR
                     QLAEWQRAGRPHYLSINVSGRQIPHGLPPAAVAEALARHGIPASRLALEITEGILLAN
                     IGEAQAWLQAVQALGVRVYLDDFGTGYSSLSYLNRFPLDTLKIDRSFVQDMATGNNAH
                     SLVIAIVAMARSVGLQVVAEGVETAGQLEILSAIGCQYAQGYYLSRPVTAADFPEVAS
                     EIDRRLATPGA"
     gene            complement(693..1529)
                     /locus_tag="PLN02_11185"
     CDS             complement(693..1529)
                     /locus_tag="PLN02_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOI52933.1"
                     /translation="MTPELREKLLTGLRDGSIVPYLGPGVLADVRDPASGAAMPADSD
                     SLIIAMNGGKPMAPKLMYEFPRAAMNVELKRGRSTVTKFLNRTYGETVWTRAALHDWL
                     KEIAPNYVIDINRDTQLQDSYAAVPHTLVVGIARIGGTDYRYKLYAWDGSSYAPVTTI
                     DLSKPVLFKPMGTPRPEGVYIASDADYVDFITELMGGFSIPPEIKEMRKGKQYLLLGM
                     RLNRDTERMVMADIIWSAAEPAGWVFISEPTTKERRYCKKIGLEIIEEDICDFIGAAV
                     AG"
     gene            1746..>2101
                     /locus_tag="PLN02_11190"
     CDS             1746..>2101
                     /locus_tag="PLN02_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52934.1"
                     /translation="MHAAERPTILVVDDTPDNLAVVAGMLEHDYRVRVAASGERGLRI
                     AAASPAPDLILLDIMMPDMDGYQTLEQLRANPATADIPVIFVTAMDSDADEAVGLALG
                     AVDYIAKPIRPAILQAR"
BASE COUNT          333 a          728 c          677 g          363 t
ORIGIN      
        1 cggcgccgag gcgctgatcc gctggcgcca cccggagcgc ggcctggtgc cgccggaccg
       61 tttcattccg ctgatcgaac aatccgggct gattgacggc ctcggcctct gggtgctcga
      121 cgatgcctgc cggcaactgg ccgaatggca gcgtgccggc cgtccccatt acctgtcgat
      181 caacgtttcc ggccgccaga ttccgcacgg cctgccgccg gccgccgtcg ccgaggccct
      241 ggcccgccac ggcatcccgg ccagccggct ggcgctggaa atcaccgagg gcatcctgct
      301 cgccaacatc ggcgaagccc aggcctggct gcaggcggtg caggcgctcg gcgtgcgcgt
      361 ctatctcgac gatttcggca ccggctactc gtcgctttcc tacctgaacc gcttcccgct
      421 cgatacgctg aagatcgacc gctccttcgt ccaggacatg gccaccggca acaacgccca
      481 ctcgctggtc atcgccatcg tcgccatggc gcgcagcgtc ggcctgcagg tggtcgccga
      541 aggcgtcgag accgccggcc agctggaaat cctcagcgcc atcggctgcc agtacgcgca
      601 gggctactac ctgtcgcggc cggtcaccgc cgccgacttt ccggaagtcg ccagcgagat
      661 cgaccgccgc ctcgccacgc cgggcgcctg attcagccgg cgacggcggc gccgatgaag
      721 tcgcagatgt cctcttcgat gatttccagg ccgatcttct tgcaatagcg ccgttccttg
      781 gtcgtcggtt cgctgatgaa cacccagccg gccggctcgg ccgccgacca gatgatgtcg
      841 gccatcacca tgcgctcggt gtcgcgattc aggcgcatgc cgagcagcag atactgcttg
      901 cccttgcgca tctccttgat ctccggcgga atcgagaagc cgcccatgag ctcggtgatg
      961 aaatcgacgt aatcggcgtc ggaagcgatg tagacgcctt ccgggcgcgg cgtgcccatc
     1021 ggcttgaaca gcaccggctt gctcaggtcg atcgtcgtca ccggcgcgta gctgctgccg
     1081 tcccaggcgt agagcttgta gcggtagtcg gtgccgccga tgcgggcgat gccgacgacc
     1141 agcgtgtgcg gcacggcggc gtaggaatcc tgcagttgcg tgtcgcggtt gatgtcgatc
     1201 acgtagttcg gggcaatttc cttcagccag tcgtggagcg cggcgcgcgt ccagacggtt
     1261 tcgccgtagg tgcgattgag gaacttggtg acggtgctgc ggccgcgctt gagctcgacg
     1321 ttcatcgccg cccgcgggaa ttcgtacatc agcttgggcg ccatcggctt gccgccgttc
     1381 atggcgatga tcagcgaatc gctgtcggcc ggcatcgccg cgccgctcgc cggatcgcgc
     1441 acgtcggcca ggacgccggg gcccagatag ggaacgatgc tgccgtcacg cagaccggtc
     1501 agcagttttt cacgcaattc gggggtcatg gttttttgct ctgtgatttg gggtagggcg
     1561 gtcaatgcaa aatccacgcc cagacagcag cgacggcgcg atgcgggttt cgccgcgcgc
     1621 cgccgggggc ggcctgtcgg gtttgcgaca aactggacaa gcccgttctg ctggcgcaac
     1681 atcgcttcat cataagaagc tggcgcagcc acctggccgc caccgccaac ggagaggcaa
     1741 acccgatgca tgcagcagag cgcccaacga tcctcgtcgt cgacgacact cccgacaatc
     1801 tggcggtggt cgccggcatg ctcgaacacg attaccgcgt ccgcgttgcg gcctcgggcg
     1861 agcgcggcct gcgcatcgcc gccgccagcc ccgctcccga cctgatcctg ctcgacatca
     1921 tgatgccgga catggacggc taccagacgc tcgaacaact gcgcgccaac ccggcaacgg
     1981 cagacatccc ggtgatcttc gtcacggcga tggacagcga cgccgacgag gcggtcggcc
     2041 tggcgctggg cgccgtcgac tacatcgcca agccgatccg cccggcgatc ctgcaggcgc
     2101 g
//
LOCUS       DAOSDX010000535         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18240_length_2098_cov_2.313754, whole genome shotgun sequence.
ACCESSION   DAOSDX010000535 DAOSDX010000000
VERSION     DAOSDX010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18240_length_2098_cov_2.313754"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLN02_11195"
     CDS             complement(<1..327)
                     /locus_tag="PLN02_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52935.1"
                     /translation="MNALPFARRFGLLVLALAMLKPALAESPGLLPELNIDLTQTTVS
                     GISSGAFMAVQFGVAHSAAVRGIAATAGGPYFCAGQDAWAGAGVGKAIARCMQGDPAY
                     PARPISA"
     gene            536..>2098
                     /locus_tag="PLN02_11200"
     CDS             536..>2098
                     /locus_tag="PLN02_11200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI52936.1"
                     /translation="MNLNTKVTLFFSAIAVSLLAVLVAISLYAFRTFSIASATDHIRS
                     AGELVRVALTESMINGVIDRRASFLSRILEVQGLRSARVVRSPEVERQFGRGLNLESQ
                     ADAIERQVMASGKPSFGVVEEDGETVFRGTIPYIATAHGTPNCLQCHQVAEGSVLGTV
                     TMTMSIDGLRDKAVLTVGGIAGAVTLFAVLLVLLARRMLRPISNTAAAVEEAVRKALD
                     GDFKTQIEQRTKDEIGQIARDMNRLLAFLDSGLNRIGTSVARLTERPATPGENLLTAT
                     IDMVEGLSKAAHFKQAIEEDETKSEIYTRLSSTLQKEFGRQEFSLYEILSHKKQMKSL
                     IVDGRPADGCRWCDPEILVRPEACRVRRTGHPVDGIDNPEICYAFQPPPEVGPRRHVC
                     FPIIQSGAVGSIVQIVCTPAEEKKLHECAPLLQVYLRETAPVLETKRLMENLRDATLR
                     DPMTGLNNRRFLEEYVDTLIAGVQRKRAHAAIMMLDLDFFKMVNDTYGHDAGDTVIKA
                     LAGVLKQAVRASD"
BASE COUNT          387 a          700 c          696 g          315 t
ORIGIN      
        1 cgcgctgatc ggccgcgccg gataggcggg atcgccctgc atgcagcggg cgatcgcctt
       61 gccgacgccg gccccggccc aggcgtcctg gccggcgcag aagtagggac cgccggcggt
      121 ggcggcgatg ccgcggacgg cggccgagtg ggcgacgccg aactggacgg ccatgaaggc
      181 gccggacgag atgccggaga ccgtggtctg cgtcaggtcg atgttgagtt cgggcagcag
      241 cccggggctt tcggccaggg cgggcttgag catggcgagg gcaaggacga gcaggccgaa
      301 gcgcctggcg aagggcagtg cgttcatctc gatgtctccc gggcggggtt gaaattgaca
      361 tcatcataga cgccgacgaa ggcctgatgc aaaaagatga tcggcgcaga aggcggcttg
      421 atcctgatca acaaagccgg cgattttccg gcggcgcccc ccttaacacc ttcgtaatag
      481 aagacggctc ggacataatg tcgcacttcc gaatccgtgt tgcgggatcg tcatcgtgaa
      541 tctcaacacc aaggtcacgc tgttcttttc ggcgattgcc gtgtcgctgc tcgccgtgct
      601 cgtagcgatc agcctctacg ccttccgcac tttctcgatc gcctcggcga ccgaccacat
      661 ccgctcggcc ggcgaactgg tgcgggtggc gctgaccgag tcgatgatca acggcgtcat
      721 cgaccgccgc gcgagcttcc tcagccgcat cctcgaagtg caaggcctgc gctcggcgcg
      781 cgtcgtccgc tcgccggaag tcgaacgcca gttcggccgc ggcctcaacc tggaaagcca
      841 ggcggacgcc atcgaacgcc aggtgatggc gagcggcaaa ccctccttcg gcgtcgttga
      901 agaggacggc gaaaccgtct tccgcggcac cattccctac atcgccaccg cccacggcac
      961 gccgaactgc ctgcaatgcc accaggtcgc cgaaggcagc gtgctcggca cggtgacgat
     1021 gacgatgtcg atcgacggcc tgcgcgacaa ggcggtgctg acggtgggcg gcatcgccgg
     1081 cgcggtgacg ctgttcgccg tgctgctggt gctgctcgcc cgacgcatgc tgcggccgat
     1141 ctcgaacacc gcggcggcgg tcgaggaggc ggtacggaag gcgctcgacg gcgacttcaa
     1201 gacgcagatc gagcagcgca ccaaggacga aatcggccag atcgcacgcg acatgaaccg
     1261 cctgctggcc ttcctcgaca gcggcttgaa ccgcatcggc accagcgtcg cccgcctcac
     1321 cgagcggccg gcgacgccgg gcgaaaacct gctgacggcg accatcgaca tggtcgaggg
     1381 gctgtcgaag gcggcgcatt tcaagcaggc gatcgaggag gacgagacca agtcggagat
     1441 ttacacccgc ctctccagca cgctgcagaa ggaattcggc cgccaggagt tctcgctcta
     1501 cgaaatcctc agccacaaga agcagatgaa gagcctgatc gtcgacggcc gcccggccga
     1561 cggctgccgc tggtgcgatc cggaaatcct ggtccgtccg gaagcctgcc gggtgcggcg
     1621 caccggccac ccggtcgacg gtatcgacaa cccggaaatc tgctacgcct tccagccgcc
     1681 gccggaagtc gggccgcgcc gccacgtctg tttcccgatc atccagtcgg gcgccgtcgg
     1741 cagcatcgtc cagatcgtgt gcacgccggc cgaggagaag aagctgcacg agtgcgcccc
     1801 gctgctccag gtctacctgc gggaaactgc gccggtgctg gaaaccaagc ggctgatgga
     1861 gaacctgcgc gatgcgacgc tgcgcgaccc gatgaccggc ctcaacaacc gccgtttcct
     1921 cgaggaatac gtcgacacgc tgatcgccgg cgtgcagcgc aaacgggcgc acgcggcgat
     1981 catgatgctc gacctcgact tcttcaagat ggtcaacgac acctacggcc acgatgccgg
     2041 cgacaccgtg atcaaggcgc tggccggcgt gctcaagcag gcggtgcgcg cctccgac
//
LOCUS       DAOSDX010000536         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18258_length_2096_cov_3.336110, whole genome shotgun sequence.
ACCESSION   DAOSDX010000536 DAOSDX010000000
VERSION     DAOSDX010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18258_length_2096_cov_3.336110"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2096
                     /locus_tag="PLN02_11205"
     CDS             <1..>2096
                     /locus_tag="PLN02_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52937.1"
                     /translation="EVSGLTAQRVATFTLIWGFMLLMIVITLWATLPGGMKAFSPEVL
                     DKFDDYLIRIFFGLAVLSSIATWWITLSEESRNRAEDELRSAKESAEAASAELARAAE
                     RTRMLLDTANDAVITIDAESMIVDWNPAAEQIFGWRRDEAIGQKMSQLIVPEPYREMH
                     EAGVRRFAETGQGSLVNQRIEITALTRDKGIIDVELSIWPVKVGDSYTFSSFVRDISR
                     RKRAEEELARARDVALEATRLKSDFLANMSHEIRTPMNAIIGMSHLALKTDLDARQRD
                     YIAKANLAATSLLRIINDILDFSKIEAGKLELERVAFALAEVCESVRTVVGGRAAEKG
                     LELLISVDPALPGHFLGDPLRLGQVLINLANNAVKFTERGRIEIAVTPQEVHGEQAVL
                     RFCVRDTGIGMTPAQLDKLFQPFSQADGSTTRKYGGTGLGLTISKRIVEAMGGEIKVE
                     SIAGVGSQFSFAVALPVLDAEQFLSQTHQTADDTLLPTGMLDGLRILLAEDNPINQQI
                     AIELMREAGAAVDVANNGRQVLEILGAATAPAYHLVLMDLQMPEMDGIEATRRVRADA
                     RFAELPIVAMTAHAMMEERQRCIDAGMVDHVGKPIDPPSFLRTIARWARYAGTQPGAG
                     APLPAAGQPAPAPVFPAIAGLDAVVGLRRVNGKTALYREMLLQFVQHQAEHDGQLRGA
                     LAAADKTQLARQLHTL"
BASE COUNT          386 a          700 c          681 g          329 t
ORIGIN      
        1 gaggtttccg ggctgacggc gcaacgcgtc gccaccttca cgctgatctg gggcttcatg
       61 ctgctgatga tcgtcatcac gctgtgggcg acgctgcccg gcgggatgaa ggcgttttct
      121 cccgaggtgc tggacaagtt cgacgactac ctgatccgca tctttttcgg cctggcggtg
      181 ttgtcgagca tcgccacgtg gtggatcacg ctctccgagg agagccgcaa tcgggccgag
      241 gacgaattgc gcagcgccaa ggaaagcgcc gaagcggcct cggcggaact ggcgcgcgcc
      301 gccgaacgca cgcgcatgct gctcgatacc gccaacgatg cggtgatcac gatcgatgcg
      361 gagagcatga tcgtggactg gaacccggcg gcggaacaga ttttcggctg gcggcgcgac
      421 gaagccatcg gccagaagat gagccagttg atcgtccccg agccctaccg ggaaatgcac
      481 gaagctggtg tgcgccgctt cgccgagacc ggccagggca gcctggtcaa ccagcggatc
      541 gaaatcaccg cgctgacccg ggacaagggc atcatcgacg tcgaactgtc gatctggccg
      601 gtcaaggtcg gcgacagtta caccttcagt tccttcgtcc gcgacatcag ccggcgcaag
      661 cgcgccgagg aggaactggc gcgggcccgc gacgtggcgc tggaagcaac gcggctgaag
      721 tcggatttcc tggccaacat gagccacgaa atccgcacgc cgatgaacgc catcatcggc
      781 atgtcgcacc tggccttgaa aaccgatctc gacgcccgcc agcgcgatta catcgccaag
      841 gccaacctgg ccgccacctc gctgctgcgc atcatcaacg acatcctcga tttctcgaag
      901 atcgaggccg gcaagctgga actcgagcgc gtcgcgttcg ccctcgccga agtgtgcgaa
      961 tcggtgcgca ccgtggtcgg cgggcgggcc gccgaaaagg ggctggaact cctgatcagc
     1021 gtcgatccgg cgctgcccgg ccatttcctt ggcgaccccc tgcggttggg gcaggtattg
     1081 atcaatctcg ccaacaacgc ggtcaagttc accgagcgcg gacgcatcga gattgccgtc
     1141 acgccgcagg aagtgcacgg cgagcaggcg gtcttgcgct tctgcgtgcg cgataccgga
     1201 atcggcatga cgccggcgca actggacaag ctgttccagc cattttcgca ggccgacggc
     1261 tcgaccaccc gcaaatacgg cggcaccggt ctcggcctca ccatcagcaa gcgcatcgtc
     1321 gaggcaatgg gcggcgagat caaggtcgag agcatcgccg gcgtcggctc acagttcagc
     1381 ttcgccgtcg cattgccggt gctcgacgcc gagcagttcc tcagtcagac gcaccagacg
     1441 gcagacgaca ccctgttgcc gaccggcatg ctcgacggac tgcgcatcct gctggcggaa
     1501 gacaacccga tcaaccagca gattgccatc gagctgatgc gggaggccgg ggcggcggtc
     1561 gatgtcgcca acaacggccg gcaggtgctg gaaatactgg gcgccgctac ggcgcccgcc
     1621 tatcacctgg tgctgatgga cctgcagatg ccggaaatgg acggcatcga ggccacccgg
     1681 cgggtgcgtg ccgacgctcg cttcgccgaa ctgccgattg tcgccatgac cgcgcacgcg
     1741 atgatggaag agcggcaacg ctgcatcgac gccggcatgg tcgatcacgt cggcaagccg
     1801 atcgatccgc cgagtttcct gcgtaccatt gcccgctggg cccgctacgc cggtacccag
     1861 cccggcgccg gcgcgccgct tcccgctgcc ggccagcccg cgccggcgcc ggtgtttccg
     1921 gcgattgccg gcctcgacgc ggtcgtcggc ctgcggcggg tgaacggcaa aacggcgctg
     1981 taccgggaaa tgctgctgca gttcgtgcag catcaggccg aacacgatgg tcagttgcgc
     2041 ggcgcgctcg ccgccgccga caagacgcag ctcgcccgcc aactgcacac cctcaa
//
LOCUS       DAOSDX010000537         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18278_length_2095_cov_2.887255, whole genome shotgun sequence.
ACCESSION   DAOSDX010000537 DAOSDX010000000
VERSION     DAOSDX010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18278_length_2095_cov_2.887255"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..793)
                     /gene="cobI"
                     /locus_tag="PLN02_11210"
     CDS             complement(11..793)
                     /gene="cobI"
                     /locus_tag="PLN02_11210"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HOI52938.1"
                     /translation="MTESKCYGKLIGASLGPGDPELITRRAWAALQSGARWLYPVKKA
                     EELSYALAIVERGGIAVPADAVELVFPMTRDAEILAKAWARAATQTVELLAEGRDLVF
                     LVEGDASTFATFGHLARVVRELAPEVEVETIPGVSSFAAAAASADVTLAEEDETFAII
                     PAAYGVEVINHLLDEFDTLALLKVKPLLDEVIDLLEGRELLATSVFIEKVGSPAERIV
                     RDIASLKGEKVNYLSLLLVQNPKRVRGELRRGCRKRQVAAVA"
     gene            complement(790..2073)
                     /gene="cbiE"
                     /locus_tag="PLN02_11215"
     CDS             complement(790..2073)
                     /gene="cbiE"
                     /locus_tag="PLN02_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HOI52939.1"
                     /translation="MSAEVFIVGILDDGWAGLGDAARTALSGAGLVIGVGRTLDLLKT
                     QLAGAEVRAMDGALARVPGWIAEARAAGRTVAVLATGDPLCHGIGGYLLGQLGGAGVR
                     VLPAPSTLQIAFARWQKPWQDVAIASCHSQDAGEWQWGATPAHGLYPLLRAVALNRRV
                     FAFTGPDNDPARLARALITAGYGDEVRLSVASRLLRADEMVACDLSAVDVAHRQFSDP
                     SVVLIERSPDAAPVFGRDDLDYLQRSPEKGLITKQEARALSLAKLRLKADSVVWDIGA
                     GSGSVGLEAAGLAPLGHVWAIEKNAADAANTRANAERFRIGNYTLGEGKAPALLDTWP
                     DPDAVFIGGSGGELDELIRLILARLKPGGRLVMNFVTLENLATATSTLKALGAGWDVV
                     QLQASRSQPILDMHRMAAQNPVWIVTAWVQNKENA"
BASE COUNT          324 a          715 c          728 g          328 t
ORIGIN      
        1 accgcaggat ttacgccacc gccgcgactt ggcgcttgcg gcagccgcgg cgcagttcgc
       61 cgcgcacgcg tttggggttc tgcaccagca gcagcgacag gtaattgacc ttctcgccct
      121 tcaggctggc gatgtcgcgg acgatgcgct ccgcgggcga gccgactttc tcgatgaaca
      181 ccgaggtcgc cagcagctcg cggccttcga gcaggtcgat gacctcgtcg agcagcggct
      241 tcactttcag cagcgccagc gtgtcgaact cgtcgagcag gtggttgatg acctcaacgc
      301 cgtaggcggc cgggatgatg gcgaaggttt cgtcttcttc ggccagcgtg acatcggcgc
      361 tggcggcagc ggcggcgaag gacgagacgc cgggaatggt ctcgacctcg acttccggag
      421 ccagttcgcg cacgacgcgg gcgaggtggc cgaaggtggc gaaggtcgag gcgtcgcctt
      481 cgaccaggaa aacgaggtcg cgcccttcgg cgagcagttc gacggtttgc gtcgcggcgc
      541 gcgcccaggc cttggccagg atctcggcgt cgcgcgtcat cgggaagacc agttcgacgg
      601 cgtcggccgg cacggcaatg ccgccgcgct cgacgatggc cagcgcgtag gacaattcct
      661 cggccttctt caccgggtac agccagcgcg cgccggattg cagcgcagcc caggcgcggc
      721 gggtgatcag ttcggggtcg ccggggccga gcgaggcgcc gatgagtttg ccgtagcatt
      781 tggattcggt catgcgtttt ccttgttctg aacccaggcg gtgacaatcc acaccgggtt
      841 ttgtgccgcc atgcggtgca tgtcgagtat cggctggctg cggctggctt gcagttgcac
      901 cacgtcccag ccggcgccga gggccttgag cgtgctcgtc gccgtcgcca ggttttccaa
      961 ggtgacgaaa ttcatcacca gccggccgcc gggtttcaaa cgggcgagga tcaggcggat
     1021 cagttcgtcc agttcgccgc cggagccgcc gatgaacacc gcgtccgggt ccggccaggt
     1081 gtcgagcagc gccggcgcct tgccttcgcc cagcgtgtag ttgccgatgc ggaagcgctc
     1141 ggcgttggcg cgcgtgttgg cggcgtcggc ggcgttcttc tcgatcgccc agacgtgacc
     1201 cagcggcgcc agcccggcgg cttcgaggcc gaccgaaccg gaaccggcgc cgatgtccca
     1261 gaccacactg tcggccttca gccgcagttt ggccagcgac agcgcgcgcg cctcctgctt
     1321 ggtgatcagg cccttttccg gcgaacgctg gaggtagtcg aggtcgtcgc ggccgaagac
     1381 cggcgcggcg tccggcgaac gctcaatcag cacgacgctg gggtcggaaa attgccgatg
     1441 ggcgacgtcg accgccgaca aatcgcaggc caccatttca tccgcccgca gcaagcggct
     1501 ggccaccgac aggcggactt cgtcgccgta gccagcggtg atcagcgccc gcgccagccg
     1561 cgccgggtcg ttgtccgggc cggtgaaggc gaaaacgcgg cggttcaatg cgaccgcccg
     1621 cagcaagggg taaagcccgt gcgccggcgt cgcgccccac tgccattcgc cggcatcctg
     1681 gctgtggcag gatgcgatgg ccacgtcctg ccagggtttc tgccagcggg cgaaagcgat
     1741 ctgcagtgtc gatggcgccg gcaacacgcg gacgccggcg ccgcccagct gaccgagcag
     1801 gtagccgcca atgccgtggc agagcgggtc gccggtcgcc agcacggcga ccgtccggcc
     1861 ggcagcgcgc gcctcggcaa tccagcccgg cacacgcgcg agcgcgccgt ccatggcgcg
     1921 cacttcggcg cccgccaact gcgttttcag caagtcgagc gtgcggccga cgccgatcac
     1981 cagcccggcg ccgctcagcg ccgtgcgggc ggcgtcgccg aggccggccc agccgtcgtc
     2041 gaggatgcca acgatgaaaa cctcagcact catcacactg cctcctcatc gacgc
//
LOCUS       DAOSDX010000538         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18290_length_2093_cov_3.557900, whole genome shotgun sequence.
ACCESSION   DAOSDX010000538 DAOSDX010000000
VERSION     DAOSDX010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18290_length_2093_cov_3.557900"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="PLN02_11220"
     CDS             <1..1491
                     /locus_tag="PLN02_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52940.1"
                     /translation="DEIVGHSVFDMAPAEIAERYEEMDRRLFAEPGTQSYEWSIMGPE
                     GRRQVIFHKATFADADGRVAGLLGGITDVSELKRVQDELQAHRDNLEALIVERTAELV
                     RAKAEAESASLAKSAFLANMSHELRTPMNGIMGMIELVWRKTGDPRDRERLAKARQSS
                     AHLLDLINGILDISKIEAERMTLEKRPFRLVEILDNALGVTAHRAEEKGLQLLVDPLP
                     ASARQALLGDPLRLSQVLINLLGNALKFTEVGSVGLRFEVLDETSASLRLRCEVSDTG
                     IGIAADALSRLFTPFEQADSSMTRRFGGSGLGLAISRRLIELMGGEIGVDSEPGRGSR
                     FWFVVTLDKPLLTGSEASACLVPESPELVLRRDFLGTRVLLAEDDPINREVACDLLED
                     VGLVIDLAADGDEAVRMASENRYDLILMDMQMPKRNGIEAAAAIRAGSLNQRTPILAM
                     TANAYDEDRQACLAAGMNDHLGKPVAPGQLFAALLQWLRAKGRAAE"
     gene            1514..>2093
                     /locus_tag="PLN02_11225"
     CDS             1514..>2093
                     /locus_tag="PLN02_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOI52941.1"
                     /translation="MILVTGGAGYIGSHTVVELLNRGESVLILDNLSNSSPRVLDRIE
                     TICGRRPAFVQGDIRDQVLLARLFAENPIESVVHFAGFKAVGESVEKPLAYFDNNVSG
                     SLCLFRAMADAGVRKLVFSSSATVYGDPHTVPIREDFPLQATNPYGRSKLMVEEILRD
                     MLCADPSWRVAMLRYFNPVGAHPSGLIGESPNG"
BASE COUNT          379 a          616 c          694 g          404 t
ORIGIN      
        1 gacgagatcg tcggtcacag cgtgttcgac atggcgccag cggaaatcgc cgagcgctat
       61 gaagaaatgg atcgccgcct gtttgccgag ccaggtacgc aaagttacga atggagcatc
      121 atggggccgg agggccggcg ccaggtgatt ttccacaagg ccacctttgc cgacgccgac
      181 gggcgggtcg ccggtctgct cggcggcatt accgatgtca gcgaattgaa gcgggtccag
      241 gacgaactgc aggcgcaccg tgacaatctg gaggctctga tcgtcgagcg tactgccgaa
      301 ctggtccggg cgaaagcgga agccgagtcg gcgagcctgg cgaaaagtgc cttcctcgcc
      361 aacatgagtc acgaattacg gacgccgatg aacggcatca tgggcatgat cgagctcgtc
      421 tggcgcaaga ccggcgatcc ccgggatcgt gagcgcctgg ccaaggcgcg ccagtcgtcg
      481 gcgcatctgc tcgacctgat caacggtatt ctcgatatct ccaagatcga agcggagcgg
      541 atgacgctgg aaaagcggcc gttccgcctg gtcgagattc ttgacaatgc cttgggggtg
      601 acggcgcacc gggcagagga aaagggcttg caactgctcg tcgatccctt gccggcaagc
      661 gcgcgccagg cattgctggg cgatcccttg cggctgtcgc aggtgctgat caacctgctt
      721 ggcaatgcgc tcaaattcac cgaagtcggt agcgttggcc tgcgcttcga ggtgctcgac
      781 gagactagcg cctcgctgcg cctgcgttgc gaggtcagcg ataccggtat cggcattgcc
      841 gccgatgcct tgtcgcgctt gtttacgccc ttcgagcagg cggattcctc gatgacgcgt
      901 cgcttcggcg ggagcgggct ggggctggcg atcagccgtc gcctgatcga gttgatgggc
      961 ggagagattg gcgtcgatag cgaacccggt cggggcagtc gtttctggtt cgtcgtgacg
     1021 ctggacaagc cgctgctcac cgggagcgaa gccagcgcct gtcttgtacc ggaatcgccg
     1081 gaactggttt tgcgtcgcga ctttctcggc acccgcgttc tgctggccga ggacgatccg
     1141 atcaaccgcg aggtggcctg cgatctgctg gaggatgtcg gattggtcat cgacctcgct
     1201 gctgacggcg atgaggccgt acgcatggcc agcgaaaatc gctacgacct gatcctgatg
     1261 gacatgcaga tgccgaagcg caacggcatc gaggccgccg ccgcgatccg cgccggatcg
     1321 ctgaaccagc ggacgccgat cctggccatg accgccaatg cctacgatga agatcgccag
     1381 gcctgcctgg ctgccggcat gaatgatcat ctcggcaagc cggtggcgcc ggggcagttg
     1441 tttgccgcct tgctgcagtg gttgcgggcg aaaggcaggg cagcggaatg aatttgtcgt
     1501 tctgggaaaa accatgattc tggtcaccgg aggcgccggc tacatcggct cgcatactgt
     1561 cgttgaactg ctcaatcgtg gcgaatcggt gctgattctc gacaatctga gcaacagcag
     1621 tccgcgggtg ctcgatcgga tcgaaaccat ctgtggccga cggccggctt tcgtgcaggg
     1681 cgatatccgg gatcaggtcc tgctggcgcg tctgtttgcc gaaaacccga tcgagtcggt
     1741 ggtccatttt gccggtttca aggcggtcgg cgaatcggtg gagaaaccgc tcgcctattt
     1801 cgacaacaac gtcagcggca gcctttgcct gttccgggcc atggccgacg ccggggtacg
     1861 caagctggtc ttcagttcgt cggcgacggt ctatggcgat ccgcacacgg tgccgatccg
     1921 cgaggatttt ccgctgcagg cgaccaatcc ttatgggcgc agcaagctga tggtcgagga
     1981 gatcctgcgc gacatgctat gcgccgatcc ttcctggcgc gttgccatgc tgcgctattt
     2041 caacccggtc ggcgcccatc ccagcggcct gatcggcgag agtccgaacg gca
//
LOCUS       DAOSDX010000539         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18313_length_2091_cov_3.245580, whole genome shotgun sequence.
ACCESSION   DAOSDX010000539 DAOSDX010000000
VERSION     DAOSDX010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18313_length_2091_cov_3.245580"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..843)
                     /locus_tag="PLN02_11230"
     CDS             complement(169..843)
                     /locus_tag="PLN02_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288518.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOI52942.1"
                     /translation="MKAFSRFVVSLAWFGVSVAQAGTTVDLAAEASRPAANDQVQAVV
                     FVEARGANPVVLARQVNQEIAGALQLIRGKAGVQVKSGNQSTYPVYGREQKIESWQMR
                     SELVLESHDLAAVSELLGELQQRKLAVGQVSQMPSPETRRKVEDEATRDAIRAFQARA
                     EVIAAALGKPWKIKQLAVSQQGGPMPVPVFRAAKAMLAEAAPAPLEAGESQLTTTVSG
                     QIELGD"
     gene            992..1951
                     /locus_tag="PLN02_11235"
     CDS             992..1951
                     /locus_tag="PLN02_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52943.1"
                     /translation="MINVLPIDRHTVDDAPPEEGSRRIINGEERVYYDGYWIKAYQVP
                     ADTLEAKKRLIDALTRRLFNHTEHGLNIPGTRLAEARAAFEAEEDAARRRIKGAMLAG
                     ALFNRAADIFRKLVELQACGIEILSDNPLMRECGRCLLEAMELGRNVLHRSGEEGIDE
                     LWGEPFRAFSIPLEDFYESRYIKIGQTMRDIDRIANCMIANIARLPAFAGAEPVILDL
                     ARAAKIKTETLRTDPDIFDVWAIMVTAGERLDALTPNPLDCNCSVEHDIAVGLQLLRQ
                     GRDLIFYITRARTPMPKSTREFVERCETFHSSGHAPMTHFRQD"
BASE COUNT          391 a          746 c          597 g          357 t
ORIGIN      
        1 gccacatgaa gaaacgcggc tgcgaggtgc tacgcaccgg cgaaaccgac ccggccctgg
       61 cgatcacccg catcctcgcc ggcgaggcgc tgtccgaccc gcgcttcgac atcaccactt
      121 cgctgtgcaa actgcgcgac ctgttctcgc gccactgaac gcagttgctt agtcacccag
      181 ctcgatctgg ccgctgaccg tcgtcgtcag ctggctttcg ccggcttcca gcggcgccgg
      241 cgcggcttcc gccagcatcg ccttggccgc ccggaagacc ggcaccggca tcggcccgcc
      301 ctgctgcgac accgctagct gcttgatctt ccagggtttg ccgagcgccg cggcgatcac
      361 ttcggcacgc gcctggaagg cgcggatggc gtcacgcgtc gcttcgtcct cgaccttgcg
      421 ccgggtttcc ggcgacggca tctggctgac ctggccgacc gccagcttgc gttgctgcag
      481 ttcgccgagc aactcggaga ccgccgccag atcgtgcgat tcgaggacca gttcgctgcg
      541 catctgccag ctttcgatct tctgctcccg gccataaacc ggataggtgg actggttgcc
      601 gctttttacc tgaaccccgg ccttgccgcg gatcagttgc agcgcgccgg cgatttcctg
      661 gttgacctgg cgcgccagca cgaccggatt ggcgccgcgc gcttcaacga aaaccaccgc
      721 ctgaacctgg tcgttggccg ccggacgcga cgcctccgcc gccagatcga cggtagtgcc
      781 ggcctgcgcc acggaaacgc caaaccaggc gagggagact acaaaccggg aaaatgcttt
      841 catgagtaaa ctcctgggtt gaccggaacc gatcataacg agcgtcccat ccggctgccc
      901 aaatccaaaa gtaaaaatat gtttcgtcct ggtaaagcgg gcacaggttc ttcttgtttc
      961 cgactctgcc ttttgtaata cttgctcccc catgatcaac gtcctcccca tcgatcgcca
     1021 taccgtcgac gacgcccccc ccgaagaagg ctcacgccgc atcatcaacg gagaagagcg
     1081 cgtctattac gacggctact ggatcaaggc ctaccaggtt ccggcggata cgctggaggc
     1141 gaagaaacgc ctgatcgacg cgctgacccg ccgcctgttc aatcataccg agcacggcct
     1201 caacatcccg ggcacccggc tcgccgaagc gcgcgccgcg ttcgaggcgg aagaggatgc
     1261 cgcccgccgc cggatcaagg gggcgatgct ggccggcgcg ctgttcaacc gcgccgccga
     1321 catcttccgc aaactggtcg aactacaggc ctgcggcatc gagatcctca gcgacaatcc
     1381 gctgatgcgc gaatgcggcc gttgcctgct ggaagccatg gaactgggca ggaacgtgct
     1441 gcaccgtagc ggcgaggaag gcatcgacga actgtggggt gaaccgttcc gcgccttctc
     1501 gatcccgctc gaggatttct acgaaagccg ctacatcaag atcggccaga cgatgcgcga
     1561 catcgaccgg atcgccaact gcatgatcgc caacatcgcc cgcctgccgg cgttcgccgg
     1621 cgccgaaccg gtcattctcg acctcgcccg ggcagccaag atcaagaccg agaccctgcg
     1681 taccgacccg gacatcttcg acgtctgggc gatcatggtc actgccggcg agcggctcga
     1741 cgccctcacc ccgaacccgc tcgactgcaa ctgcagcgtc gaacacgata tcgccgtcgg
     1801 cctccagcta ctgcgccagg gccgcgacct gattttctac atcacccgcg cccggacgcc
     1861 gatgcccaag agcacccggg aattcgtcga gcgctgcgaa actttccaca gcagcggcca
     1921 cgcaccgatg acccatttcc gccaggactg aagacccttc agttcgattc gcagcgattg
     1981 cggccgctcc ccttggcctt gtacagcaat tcatcggcct gacggaacac atccttgacc
     2041 atgctgctgg ctggcgacac gcagaccgcg ccgaccgaaa ccgtgacccg c
//
LOCUS       DAOSDX010000540         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18317_length_2091_cov_2.641454, whole genome shotgun sequence.
ACCESSION   DAOSDX010000540 DAOSDX010000000
VERSION     DAOSDX010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18317_length_2091_cov_2.641454"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PLN02_11240"
     CDS             complement(<1..1116)
                     /locus_tag="PLN02_11240"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HOI52944.1"
                     /translation="MPQLRRLLLALILSIAGNVFAQSVLFIATSPVPPGKFKVITEIA
                     RKHGLNVEAHFAERIPPEKLDALFDGRDAVIFDAARSHMQDAVRRRLEKPLARLNTRH
                     IWMRDNGPLAAGFTQPQAERLHTYYTNGGRANFDGFFATLAAELRGQPAPKPAPKLAP
                     PFVFPEDAIYHPQAPNQVFATPAEYFRWRGIDLEKRPPTVAIAFHQIYIGAEQTAFID
                     DLIARIEAGGAIALPYYAGVMTSHAPMLTVAGKPVADALINTQIILNPEGWRQELEKL
                     GIPVLQALAYRRGDAAAWRADAQGLPMIDVPFYLSQGEYAGIIDAMVTNAAGKDGQVV
                     VLPEQTAAVVNKALKLVRLQRLANAHKKVPMLYWNYLP"
     gene            complement(1172..>2091)
                     /locus_tag="PLN02_11245"
     CDS             complement(1172..>2091)
                     /locus_tag="PLN02_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI52945.1"
                     /translation="NSAWTLIPGIRYDYTRLKPSVDALAQHALTANGREAVKQSHDAV
                     SPKLGARWELNPQFTLFGQIASGFRAPNYSEVNGSFRNLTMFYGTTPNPDLKPETSVG
                     IELGMRFKRDAMRAQFTVYDNRYKDFIETVRLSCPADPLCIPGITTYAAMNLNKVRIY
                     GAELKGSWEISERWRVDGGLAYAHGENQESHQPLNSIEPVRLSAGVSYETGKWGGETR
                     LRAAQRKKRTDDSDGTWFRPAGYGVTDLSVWFRPTRETRIVATVNNLFDKKYWLWSDI
                     RQADASNPPGVDFYSQPGRSFRLSLQADF"
BASE COUNT          316 a          589 c          784 g          402 t
ORIGIN      
        1 cggcagatag ttccagtaca gcatcggcac cttcttgtgg gcgttggcca ggcgctgcag
       61 gcgaaccagc ttgagcgcct tgttgacgac ggcggcggtc tgctcgggca aaacgacgac
      121 ctgaccgtcc ttgccggcgg cgttggtgac catcgcgtcg atgatgccgg cgtattcgcc
      181 ctgcgacagg tagaagggga catcgatcat cggcaggccc tgcgcgtcgg cccgccaggc
      241 ggccgcgtcg ccgcgacggt aggcgagtgc ctgaagcacc ggaatgccga gcttttccag
      301 ttcctggcgc cagccttcgg ggttgaggat gatctgcgtg ttgatcagcg cgtcggcgac
      361 cggcttgccg gcaacggtta gcatcggtgc atgcgaggtc atgacgccgg cgtagtacgg
      421 cagggcgatg gcgccgccgg cttcgatgcg ggcgatcagg tcgtcgatga aggcagtctg
      481 ctcggcgccg atgtagatct ggtggaaggc gatggccacc gtcggcggcc gcttttcaag
      541 gtcgatgccg cgccagcgga agtattcggc cggcgtcgcg aacacctggt tgggggcttg
      601 cgggtggtag atggcgtctt ccgggaagac gaagggtggc gccagcttgg gcgccggctt
      661 gggcgccggc tggccgcgca gttcggcggc gagggtggcg aagaaaccgt cgaaattggc
      721 gcgcccgccg ttggtgtaat aggtgtgcag gcgttcggcc tgcggctggg tgaacccggc
      781 ggcgagcggg ccgttgtcgc gcatccagat gtgccgggtg ttcagccggg ccagtggttt
      841 ttccagccgg cgcctgaccg cgtcttgcat gtggctgcgc gcggcgtcga agatgaccgc
      901 gtcgcggccg tcgaacagcg cgtcgagttt ttcgggcgga atcctctcgg cgaaatgcgc
      961 ttcgacgttc agcccgtgtt ttcgggcgat ttccgtgatc accttgaact tgcctggcgg
     1021 tactggcgag gtggcgatga agaggacgga ctgggcgaag acgttgccgg cgatactgag
     1081 gatcagggcg agcaggagac ggcgaagctg gggcatggtt ggcctggcgg ggaaacggaa
     1141 ggccggcacc agtgggcgcc ggcgggcggg atcagaaatc tgcctgcagc gagaggcgga
     1201 agctgcgtcc cggttgcgag tagaaatcga cgcccggcgg gttgctcgcg tcggcctggc
     1261 ggatgtcgct ccacagccag tatttcttgt cgaagaggtt gttcaccgtg gcgacaatgc
     1321 gcgtttcccg ggttggccgg aaccagacgg agaggtcggt gacgccatag ccggccgggc
     1381 ggaaccaggt gccgtcgctg tcgtcggtgc gcttcttgcg ctgcgcggcg cgcaggcggg
     1441 tttcgccccc ccatttgcca gtctcgtagc tgacgccggc cgacaagcgg accggttcga
     1501 tactgttcag cggctgatgg ctttcttgat tctcgccgtg ggcgtaggcg agaccgccgt
     1561 ctacgcgcca gcgttcgctg atctcccaac tgcccttgag ttcggcgcca tagatgcgga
     1621 ccttgttcag gttcatcgcg gcgtaggtgg tgatgccggg aatgcacagc ggatcggccg
     1681 ggcaggagag acggacggtt tcgatgaagt ccttgtagcg gttgtcgtac acggtgaact
     1741 gggcgcgcat ggcgtcgcgc ttgaaacgca tgccgagttc gatgccaacg ctggtttccg
     1801 gcttcaggtc gggattgggc gtggtgccgt agaacatcgt caggttacgg aagctgccat
     1861 tgacctcgct gtagtttggc gcccggaaac cggaagcgat ctgcccgaac agggtgaatt
     1921 gcgggttgag ttcccagcgc gcgccgagct tcggcgacac cgcgtcgtgc gactgcttga
     1981 cggcttcccg gccgttggcg gtgagcgcgt gctgggccag cgcatcgacg ctgggcttga
     2041 gcctggtgta gtcgtagcgg atgccgggaa tcagcgtcca ggcgctgttg g
//
LOCUS       DAOSDX010000541         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18357_length_2088_cov_2.636990, whole genome shotgun sequence.
ACCESSION   DAOSDX010000541 DAOSDX010000000
VERSION     DAOSDX010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18357_length_2088_cov_2.636990"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLN02_11250"
     CDS             complement(<1..242)
                     /locus_tag="PLN02_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HOI52946.1"
                     /translation="MTRKIRIADLPAFDMAEHLPDEQAVAEYLTVVLEENDPAALAAA
                     LGTIARARGMSDIAKASGITREALYKALRAEASPRF"
     gene            complement(246..542)
                     /locus_tag="PLN02_11255"
     CDS             complement(246..542)
                     /locus_tag="PLN02_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOI52947.1"
                     /translation="MYSIKLLPEFSTWLNGLKDGMTHRRLARRLEKAQQGNLGDVKPV
                     GHGVFEMREHFGPGWRMYYVQRGEVLIVMLGGGDKSTQSADIAKAAGLADMLEE"
     gene            complement(643..939)
                     /locus_tag="PLN02_11260"
     CDS             complement(643..939)
                     /locus_tag="PLN02_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52948.1"
                     /translation="MKPSLIAPIALVCLALAGCMPGTKERMMQRGFDAAYSQGYEDGV
                     ASGKNAAGGLGYQLKKDTRSFANDAQYRQGWSDGYDAGKTQELEWQKQLKKTAK"
     gene            1034..1588
                     /locus_tag="PLN02_11265"
     CDS             1034..1588
                     /locus_tag="PLN02_11265"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOI52949.1"
                     /translation="MTQATFFRAPSNLPKLARRLASGCLLAIVLLPLSVVFYWMLSDA
                     GTLAVRANLAPGAVQGELQTWQRLLGCLLMEVPLCLLLVGVWQARKCFLLLGSGRVFT
                     AESIGYLRSFAAWSVASAIARIFCDAAASVVVTLANLPGQRMLALGLGSDQFFLLFFA
                     ALVWLMAGVIGEGLALADENASFV"
     gene            1605..1850
                     /locus_tag="PLN02_11270"
     CDS             1605..1850
                     /locus_tag="PLN02_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI52950.1"
                     /translation="MPIVVRIDVMLAKRKARSKDLAEYVGITEANISLLKQGKVKGVR
                     FETLERICEFLACQPGDLLVHVPAGAGIDDAMEPGGG"
     gene            1862..>2088
                     /locus_tag="PLN02_11275"
     CDS             1862..>2088
                     /locus_tag="PLN02_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52951.1"
                     /translation="MTSRLFPALALSLLLHLALAIAVRPSPAPRAAQVVVLAARLSPA
                     APVVRADPPPAPVESKRASRQPAARRAPSETA"
BASE COUNT          304 a          634 c          660 g          490 t
ORIGIN      
        1 tcgaagcgcg ggctggcctc ggcgcgcagc gccttgtaga gcgcctcgcg ggtgattccc
       61 gaagccttgg cgatgtcgct catgccgcgc gcccgggcaa tcgtccccag cgccgccgcc
      121 agcgccgccg gatcgttctc ttcgaggacg acggtgaggt actcggcaac cgcctgctcg
      181 tccggcaaat gctcggccat gtcaaacgcc ggcaggtcgg cgatcctgat ttttctggtc
      241 atcgttcact cctcaagcat gtcggccaat ccggcggcct tggcaatgtc ggcgctttgc
      301 gtcgatttgt cgccgccacc cagcatcacg atcagcactt cgccgcgttg cacgtagtac
      361 atgcgccagc cgggtccgaa atgctcgcgc atctcgaata cgccgtgacc gaccggtttg
      421 acgtcgccga gattgccctg ctgggctttt tccagacgtc gcgccagacg gcggtgcgtc
      481 atgccgtcct tgaggccgtt cagccatgtg ctgaactcgg gaagcaattt gatcgagtac
      541 atgagtagtg tagtcgttcg attacataca ggcaattgac gatgtctgtc gactgttgcc
      601 cttgggctgg agtgaccgcg gcagaaaagg tcggttttcc tgctatttcg ctgttttttt
      661 gagctgcttc tgccactcga gctcctgcgt ctttccagcg tcgtagccat cgctccagcc
      721 ttgacggtac tgcgcgtcgt tggcaaagct ccgggtgtct ttcttcaatt ggtagccgag
      781 gccgcctgcg gcgttcttgc cgctggcgac gccatcctcg tagccctggc tgtaggctgc
      841 gtcaaatccc ctttgcatca ttcgttcctt ggttccgggc atgcatccgg ccaaagccaa
      901 acagacaagt gctatcggcg cgataaggct tggtttcatt attgctgctc cagtctagcg
      961 ttgaaaatta ttgttttaca gaaaaatatt attgcattac gattggttgt gcaaattttt
     1021 cgtggagcat tcgatgacac aagcgacttt tttccgggcc ccgtccaatc tccctaaact
     1081 cgctcgccgt ctggcttcgg ggtgtctgct ggcgattgtc ctgttgccct tgtcggtcgt
     1141 cttctactgg atgttgagcg acgccggaac gctggcggtg cgggccaatc ttgcgcctgg
     1201 tgccgtgcag ggagaactgc agacctggca gcgcctgctc ggctgcttgt tgatggaggt
     1261 gccgctgtgc ttgttgctgg tcggcgtctg gcaggcgcgc aagtgtttcc tgctgctcgg
     1321 cagcggccgg gtattcaccg ctgagtcgat cggctatctg cgcagttttg ccgcctggtc
     1381 ggtggcttcg gcgattgcca ggattttctg cgatgcggcg gcctcggtgg tggtgacgct
     1441 ggcgaacctg cccgggcagc gcatgctggc gctcgggctg ggctcggatc agttcttcct
     1501 gttgttcttt gctgcgctgg tatggctgat ggctggcgtc atcggcgagg gcctggcgct
     1561 ggccgacgaa aacgcgtcat tcgtctgact ggccgggaac gctcatgccc atcgtcgtcc
     1621 gcatcgacgt catgctcgcc aaacgcaagg ccaggtcgaa ggatcttgcc gagtacgtgg
     1681 gcatcaccga agccaacatt tccctgctca agcagggaaa ggtcaagggg gtccgcttcg
     1741 agaccctgga aaggatctgc gaattcctcg cctgccagcc cggcgacttg ctcgtccatg
     1801 ttccggcggg agcggggatt gacgacgcga tggagcctgg cggcggctga cggggtgcgt
     1861 cgtgacgtcc cggctgtttc cggccctggc gctttcgctg ttgctgcatc tggcgctggc
     1921 gatcgctgtt cgtccctcac cagcgccccg ggcggcgcag gtcgtggtgt tggcggcgcg
     1981 tttgtcgccg gctgcgccgg tcgtccgggc cgatccgccg ccggcgccgg tcgagtcgaa
     2041 acgcgcaagt cggcagcctg ccgccagacg cgccccgagc gaaactgc
//
LOCUS       DAOSDX010000542         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18376_length_2086_cov_3.015756, whole genome shotgun sequence.
ACCESSION   DAOSDX010000542 DAOSDX010000000
VERSION     DAOSDX010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18376_length_2086_cov_3.015756"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..914)
                     /locus_tag="PLN02_11280"
     CDS             complement(129..914)
                     /locus_tag="PLN02_11280"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOI52952.1"
                     /translation="MNEESCLLRDDRADGLTTLTLNRPGQFNSLSREMLTALQSELDA
                     IAADEKVRVVVIAGAGKAFCAGHDLKEMRANHSKEFMQALFRQCGELMLTITRLPQPV
                     IARVHGIATAAGCQLVSMCDLAVAANVAKFAVSGINVGLFCSTPAVGLARNLGRKAAL
                     EMLLTGEFIDAMEARAKGLVNRVVPADALDAEIERLAASILAKSAVAVRMGKGMFYKQ
                     LEMGLADAYDYAGEVMACNMMSEDAGEGIDAFMQKRKPAYKGC"
     gene            1031..>2086
                     /locus_tag="PLN02_11285"
     CDS             1031..>2086
                     /locus_tag="PLN02_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HOI52953.1"
                     /translation="MRRLFSVSHLTAGFIAVLVGYTSSAAIVFQAASAAGAGAELIGS
                     WLWALGIGMFATSVGLSLWFKAPVLTAWSTPGAALLAVGLSGVPMSEAIGAFLFASAL
                     IVVCGVFGLFEAIMRHVPKSLAAALLAGVLVRFGMALFTSLGSELLLVGAMFAVYFAG
                     RRWLPRYAVPLSFLVGLLLAAAGGMIHAEAVSLTLALPVWTTPTFSLTTMIGVGLPLF
                     IVTMASQNVPGLAVLRANGYSTPASPLITWTGVTGLLLAPFGGFAFNLAAITAAICMS
                     PAADPDPAQRYRASVWAGVFYLFTGLFGATVAALFGALPAALIAAIAGLALLGTIGNS
                     LGAALADEREREAALVTF"
BASE COUNT          253 a          710 c          717 g          406 t
ORIGIN      
        1 ccgagtacgt cgagtcgccg gaggtgctga gcgaactcga gcgcatggaa atccaccgcg
       61 cccagggtta ctacatcggc cgcccggccc cggccatcct gccccgggac tggctgccgc
      121 cggcctgatc agcagccctt gtaggccggc ttgcgcttct gcatgaaagc gtcgatgcct
      181 tcgccggcat cctcgctcat catgttgcag gccatcacct cgccggcgta gtcgtaagcg
      241 tcggcgaggc ccatttccag ttgcttgtag aacatcccct tgcccatgcg tacggcgacg
      301 gcgctcttgg cgaggatgga ggcggctagg cgttcgattt cggcgtccag cgcgtcggcc
      361 ggcacgacgc ggttgaccag gcccttggcg cgggcttcca tggcgtcgat gaattccccg
      421 gtgagcagca tttccagcgc cgccttgcgc cccaggttgc gtgccaggcc gaccgccggc
      481 gtcgagcaga agaggccgac gttgatgccg gagacggcga acttggcgac gttggcagcc
      541 accgccaggt cgcacatcga caccaactgg cagccggcag cggtggcgat gccatgcacg
      601 cgggcgatca ccggttgcgg caggcgggtg atggtgagca tcagctcgcc gcactggcgg
      661 aacagcgcct gcatgaattc cttgctgtgg ttggcgcgca tttccttgag atcgtggccg
      721 gcgcagaagg ccttgccggc gccggcgatg acgacgacac ggaccttctc gtcggcggcg
      781 atggcgtcga gttcgctctg cagcgccgtc agcatctcgc gcgacagcga gttgaactgg
      841 cccggccggt tcagggtcag cgtggtcagg ccgtcggccc ggtcgtcgcg caacaggcag
      901 gattcttcat tcatgcgggt ctcctctatc ggttttgttg gtgagttgcg actgtaaccg
      961 agaagccggg cgggctccag ccgtcggccg cctgctatga tgccgggctg atttcccgga
     1021 cgattccgcc atgcgccgcc tgttttccgt ttcccacctg accgccgggt tcattgccgt
     1081 cctcgtcggc tacaccagtt cggcggccat cgtcttccag gcggcgagcg ccgccggggc
     1141 aggtgccgaa ctgatcggct cgtggctgtg ggcgcttggc atcggcatgt tcgccacttc
     1201 ggtcggcctg tcgctgtggt tcaaggcgcc ggtgctgact gcctggtcga cgccgggggc
     1261 ggcgctgctc gccgttggtc tcagcggcgt gccgatgagc gaggcgatcg gcgctttcct
     1321 cttcgcttcg gcgctgatcg tcgtctgcgg cgttttcggg ctgttcgagg cgatcatgcg
     1381 ccacgtcccg aaatctctgg cggcggcgct gctggcgggc gtcctggtgc gcttcgggat
     1441 ggcgctgttc acctcgctgg gtagcgagtt gctgctggtc ggagcgatgt tcgcggtcta
     1501 ttttgccggc cgtcgctggc tgccgcgcta cgccgtgccg ctttccttcc tcgttggcct
     1561 gctgctcgcc gcggccggcg gcatgatcca cgccgaagcg gtgagcctga cgctggcgct
     1621 gccggtgtgg acgacgccga cgttttcgct gaccacgatg atcggcgtcg gcctgccgct
     1681 gttcatcgtc accatggcca gccagaacgt tcccggcctg gcggtcctgc gcgccaacgg
     1741 ctactcgacg ccggcctcgc cgctgatcac ctggaccggc gtcaccggcc tgctgctggc
     1801 gccgttcggc ggttttgcct tcaacctggc ggcgatcacc gccgccatct gcatgagccc
     1861 ggcggccgat cccgacccgg cgcagcgcta ccgggcgtcg gtctgggccg gcgtcttcta
     1921 cctgttcacc ggcctcttcg gcgccaccgt cgccgcgctg ttcggcgcgc tgccggcggc
     1981 gctgatcgcg gcgattgccg ggctggcgct gctcggcacc ataggcaaca gcctcggcgc
     2041 ggcgctcgcc gacgagcgcg agcgggaagc ggcgctggtc accttc
//
LOCUS       DAOSDX010000543         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18377_length_2086_cov_2.915313, whole genome shotgun sequence.
ACCESSION   DAOSDX010000543 DAOSDX010000000
VERSION     DAOSDX010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18377_length_2086_cov_2.915313"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /gene="murC"
                     /locus_tag="PLN02_11290"
     CDS             complement(<1..889)
                     /gene="murC"
                     /locus_tag="PLN02_11290"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOI52954.1"
                     /translation="MRHKVKHIHFVGVGGSGMSGIAEVLAHLDYTVSGSDIAASATTR
                     RLEGEGVRVTIGHAADNIAGADAVVVSTAVKEDNPEVVAARDKRIPVVPRAQMLAELM
                     RLKRGIAIAGTHGKTTTTSLVTSILAEGGIDPTFVIGGRLNAAGANARLGSGDFLVAE
                     ADESDASFLFLSPVISVVTNIDADHMETYGHDFERLKGAFVDFLNRLPFYGVAVLCAD
                     DANVRAIMPQVSKQIVSYGLSPDANIRAENIVADGAQMRFTCVRVNGSESRLEITLNT
                     PGMHNVLNALAAIAVATEVG"
     gene            complement(886..1944)
                     /gene="murG"
                     /locus_tag="PLN02_11295"
     CDS             complement(886..1944)
                     /gene="murG"
                     /locus_tag="PLN02_11295"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOI52955.1"
                     /translation="MSKTILIMAGGTGGHIFPALAVAHKMRDAGWRVVWLGNPQGMEA
                     RLVPQHGFEMVWLDFSALRGKGLARKLLLPVNLLRGFWQGLKAIRQVKPNVVLGMGGY
                     ITFPAGMMAALSGVPLVLHEQNSVAGLANRVLAGVADRIASGFPEVLKKGVWLGNPVR
                     PEIAAVKPPAERFAGRDGALRLLVIGGSLGAQALNETVPQGLSRLTAEQQPQIVHQAG
                     EKHIDALKAAYAAVGVQAHCVPFIEDMAGAYEWADLVICRAGALTIAELAAVGVASIL
                     VPFPHAVDDHQTANARFLVNVGGAFLLPQRDLTPDAVALIRNYSRSQLLEMAEKARSL
                     AKPDATLDVVNLCIEAAK"
BASE COUNT          353 a          694 c          679 g          360 t
ORIGIN      
        1 cgccgacttc ggtggcgacg gcaatcgccg ccagcgcatt gagcacgttg tgcatgcccg
       61 gcgtgttcaa ggtgatttcc aggcgcgact ccgaaccgtt cacgcgaacg caggtgaagc
      121 gcatctgcgc gccgtcggcg acgatgttct cggcgcggat gttggcatcc ggggacaggc
      181 cgtagctgac aatctgcttg cttacctgcg gcatgatcgc ccgcacgttg gcgtcgtcgg
      241 cgcaaagcac ggcgacgccg tagaacggca ggcggttgag gaaatcgaca aaggcgccct
      301 tgagccgctc gaagtcgtgg ccgtaggtct ccatgtggtc ggcgtcgatg ttggtgacca
      361 ccgaaatcac cggcgacagg aagaggaagg aagcatcgga ttcgtcggcc tcggccacca
      421 ggaaatcacc gctgcccagg cgcgcgttgg cgccggcggc attcaggcga ccaccgatga
      481 cgaaggtcgg gtcgatgccg ccttcggcca ggatgctggt caccaggctg gtcgtagtcg
      541 tcttgccgtg cgtgccggcg atggcgatgc cacgcttcag gcgcatcagc tcggccagca
      601 tctgcgcccg cggcaccacc gggatgcgct tgtcgcgggc ggcgacgact tccgggttgt
      661 cttccttgac tgccgtcgaa acgactaccg cgtcggcccc ggcgatgttg tccgcggcat
      721 ggccgatggt cacccgcacg ccttcacctt ccagacggcg cgtcgtcgcg ctggcggcaa
      781 tgtcggaacc gctgaccgta tagtcgaggt gggcgagcac ttcggcgatg ccgctcatgc
      841 ccgagccgcc gacgccgacg aagtggatgt gtttgacctt gtgtctcatt tggctgcttc
      901 gatacaaaga ttgaccacgt ccagcgtggc gtccggcttg gccaggctgc gggccttctc
      961 ggccatttcc agcaactgac tgcgcgaata attgcggatc aacgccaccg cgtccggcgt
     1021 cagatcgcgc tgcggcaaca ggaaggcgcc gccgacattg accaggaaac gggcattggc
     1081 ggtctgatga tcgtccaccg catgcgggaa gggcaccagg atgctggcca cgccgaccgc
     1141 cgccagttcg gcgatggtca gcgcgccggc ccggcagatc accagatcgg cccactcgta
     1201 ggcgccggcc atgtcctcga tgaagggcac gcagtgggcc tgcacgccga ccgcggcgta
     1261 ggccgccttc agggcatcga tgtgcttctc gccggcctga tggacaatct gcggctgctg
     1321 ttcggcggta agccgcgaca ggccttgcgg cactgtctcg ttgagcgcct gggcgcccag
     1381 gctaccgccg atcaccagca ggcgcagcgc cccgtcgcga ccagcgaaac gctcggccgg
     1441 cggcttcacg gcggcgattt ccggacgcac cggattgccc agccagacgc ctttcttcag
     1501 cacctccggg aagccgctgg cgatgcgatc ggcaacgccg gccagcaccc ggttggccag
     1561 cccggcgacc gaattctgtt cgtgcagtac cagcggcacg ccgctcagcg ccgccatcat
     1621 gccggccggg aaagtgatgt aaccgcccat gccgagcacc acgttcggct tgacctggcg
     1681 gatcgccttg aggccctgcc agaaaccgcg caggagattc accggcagca acagcttgcg
     1741 cgccagcccc ttgccgcgca gcgcggaaaa atcgagccac accatctcga agccgtgctg
     1801 cggcaccagg cgcgcttcca tgccctgcgg attgcccagc cagaccacgc gccagccggc
     1861 atcgcgcatc ttgtgggcga cagccagggc gggaaaaata tggccgccgg taccgccggc
     1921 catgatcagg atggttttag acatggcacc ccccttcgca tttggcgcct gcggtttgcc
     1981 acagcctgtt ttgtctccgg ccgcgctgcg cgcttaactt gctgcgcaag ttagcctgcg
     2041 acggaacagg caattgatgg attcgggctt tcggtttcat aatttg
//
LOCUS       DAOSDX010000544         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18416_length_2083_cov_2.698718, whole genome shotgun sequence.
ACCESSION   DAOSDX010000544 DAOSDX010000000
VERSION     DAOSDX010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18416_length_2083_cov_2.698718"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..909)
                     /locus_tag="PLN02_11300"
     CDS             complement(142..909)
                     /locus_tag="PLN02_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HOI52956.1"
                     /translation="MPLPTHPKAVLIDLAGVMHTGDAALPGAVAALARLRGSGLALRF
                     LTNTTRTPGSVLFTKLCRMGFTLAAGEIQTAALAARTLVRERGLRPHWMVHPNIAEEM
                     GASHPEPDVVVLGDMGEHFSYAGLNHAFRLLMQGVPLLAMARNRYFMEPDGLALDMGA
                     FVAGLEYSAGVRAEVVGKPAPAFFAAALAELGVSAGDAVLIGDDLADDIGGAQDSGIA
                     AILVRSGKFRPGDETHPEIRPALIVDDFAAAVEVLIG"
     gene            1021..1794
                     /locus_tag="PLN02_11305"
     CDS             1021..1794
                     /locus_tag="PLN02_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI52957.1"
                     /translation="MSASSLAVTGWFPVWQRNFLVWRKLALPSILGNLADPLIYMLGL
                     GYGLGGMLPEIGGQPYIAFLAAGTIGASTMNAATFEALYSSFSRMHVQKTWDAILNTP
                     LGLTDVVAGELFWAATKSFFSGCAILVVVTVMGLTHGPLALLILPAIVLTGLAFAALG
                     LIWNALAPSYDFFMYYFTLFITPMTLISGVFFPTDQLPPWLAAVGGWLPLAQGVALIR
                     PLLAGQWPADPALHVGVLLATSAVAFAIDLRLTRRRLLK"
     gene            1804..>2083
                     /gene="cutA"
                     /locus_tag="PLN02_11310"
     CDS             1804..>2083
                     /gene="cutA"
                     /locus_tag="PLN02_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOI52958.1"
                     /translation="MDNLLILTNCPDETVANAIALAVVEEGLAACVNLLPRAQSIYRW
                     QGVVESASEVPLLIKASAANYPALEQRIAELHPYELPEIIALPITRGLP"
BASE COUNT          325 a          778 c          630 g          350 t
ORIGIN      
        1 cggcgagacc gccttcttct actgccgcga cgcccagccg ctgctcgccg cactggccac
       61 cgccgacggc ctgcgctatc tgcaccgcgc cgccaacctg gaagacgtct tcatcaagct
      121 gaccggccgc gaactgcgcg attagccgat cagcacctcg accgccgccg caaaatcgtc
      181 aacgatcagc gccgggcgga tttccggatg cgtctcgtcg cccggacgaa atttgccgct
      241 gcgcaccagg atggcggcaa tacccgagtc ctgcgcgccg ccgatgtcgt cggcgagatc
      301 gtcgccgatc agcaccgcgt cgccggcgct gacgccaagc tcggcgagcg ccgcggcaaa
      361 gaaggccggc gccggcttgc cgacaacctc ggcgcggacg ccggccgaat attccagccc
      421 ggcgacgaag gcgcccatgt ccagtgccag cccgtccggc tccatgaaat agcggttgcg
      481 cgccatcgcc agcagcggca cgccctgcat cagcaggcgg aaagcatggt tgagaccggc
      541 atagctgaag tgttctccca tgtcgccgag aacgacaacg tccggctcgg ggtgactggc
      601 gcccatctcc tcggcgatgt tcggatgcac catccagtgc gggcgcaggc cacgctcgcg
      661 aaccagggtg cgggcggcca gcgcggcggt ctgaatctcg ccggcggcga gcgtgaaacc
      721 catccggcac agcttggtga acagcacgct gcctggcgtg cgcgtcgtgt tggtcaggaa
      781 acgcagcgcc agaccgctgc cgcgcagcct ggccagtgcc gccacggcgc caggcagggc
      841 agcgtcgccg gtatgcatga cgccggccag gtcgatcagg acagctttcg gatgagttgg
      901 caatggcatc gtcttctccc gcgcggcgtg aaaatgcatc ataggcgatg accgcggcaa
      961 gcgcgaaaag tttgccgcgc ggcgcggtag aatgcgcatt ccgccctccc ggcccgcacc
     1021 atgtctgcat cctccctcgc cgtcaccggc tggttccccg tctggcagcg caacttcctg
     1081 gtctggcgca agctcgcgct gccttccatc ctcggcaacc tggccgatcc gctgatctac
     1141 atgctcggcc tcggctatgg cctgggcggc atgctgccgg aaatcggcgg ccagccctac
     1201 atcgccttcc tcgccgccgg cacgatcggc gcctcgacga tgaacgccgc caccttcgaa
     1261 gcgctctatt cgtcgttctc gcgcatgcac gtgcagaaga cctgggacgc catcctcaac
     1321 acgccgctcg gcctgaccga cgtcgtcgcc ggcgaactgt tctgggcggc gaccaaatcc
     1381 ttcttctccg gctgcgccat cctcgtcgtc gtcaccgtca tgggcctcac ccacgggccg
     1441 ctggcgcttc tcatcctgcc ggccatcgtc ctcaccgggc tggctttcgc cgcgctcggc
     1501 ctgatctgga acgcgctggc accgtcctac gatttcttca tgtactactt cacgctgttc
     1561 atcacgccga tgacgctgat ttccggcgtc ttcttcccga ccgaccagtt gccgccctgg
     1621 ctcgccgccg tcggcggctg gctgccgctg gcccagggtg tcgcgctgat ccgtccgctg
     1681 ctcgccgggc aatggccggc cgacccggcg ctgcacgtcg gcgtgctgct ggcgaccagc
     1741 gccgtcgcct tcgccatcga cctgcgcctg acccggcgcc gcctgctcaa gtagaattcg
     1801 ccgatggata atctgctcat cctgaccaac tgccccgacg aaaccgtcgc caacgccatc
     1861 gccctggccg tcgtcgaaga aggtctcgcc gcctgcgtca acctcctgcc gcgcgcgcag
     1921 tcgatctacc gctggcaagg cgtcgtcgag tcggcatcgg aagtcccgct gctgatcaag
     1981 gcgagcgcgg caaactaccc ggcgctcgaa caacggatcg ccgaactgca tccctacgag
     2041 cttcccgaga tcatcgccct gcccatcacg cggggcttgc cgg
//
LOCUS       DAOSDX010000545         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18450_length_2079_cov_3.538538, whole genome shotgun sequence.
ACCESSION   DAOSDX010000545 DAOSDX010000000
VERSION     DAOSDX010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18450_length_2079_cov_3.538538"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..>2079)
                     /locus_tag="PLN02_11315"
     CDS             complement(44..>2079)
                     /locus_tag="PLN02_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI52959.1"
                     /translation="CSFHPADTRPPAVGSGEISLAIEHDAVLCGWLHLALAPDVSDDD
                     EERLLREDIGVELGLGIGKMRARQRLASSEASLRQLSLAIEQSPHGVIITNREHGIEY
                     VNQAFIAITGYPAAEVIGKPPAILAEGLTAQNVFAEIDACIRRGEIWRGELLSRRRDG
                     YFYDIRAIITPVRQADSEVTHYLAIIEDITERKRNLAELARYREQLESLVAQRTRQLL
                     QAKEDAEAASRSKSRFLANMGHEIRTPMNAILGLTHLLQRDIGDSVVSERLARIADAA
                     NQLMQLFNDILDLSRLEAGNLTPADNEFPLPELIAEVSQQFAEKAGSKNIIIDRQFAA
                     GIPEKLRGDAPHIRQILQQLLSNAVKFTDSGRITIAVSNPDRDGEHLLLRFSVSDTGI
                     GIADEVSARLFHPFEQGDDSTTRRHGGVGLGLVISRRLVELLGGEIGVNSAPGLGSEF
                     WFTLPLRAVDSRQPDLATRPPDADPAAAGDPLERIPGLDYKLGLHAVRGKQAVYERLL
                     NTFAGSHLDDFEKIRELLLCGEEEEARRLAHSIKGAAGTLGATRVFEAAAAVDHAIRQ
                     QRPANEVLDLIDVLDGAYHVLHDALARLAANTEPAAVATEVLGAEQIRSRLDGLVRQL
                     IDGDFAVQSRLQRDAEVLRQALGGMHPAFERKIAEYDFPAAAELLEQALQKLG"
BASE COUNT          292 a          621 c          784 g          382 t
ORIGIN      
        1 ggccacgtcg ccggcgacgg cgtgcgcgcc tggctgggct gactcagccg agcttctgca
       61 gcgcctgttc gagcagttcc gcggcggccg ggaagtcgta ttcggcgatc ttgcgttcga
      121 aggccggatg catgccgccg agcgcctggc gcagcacctc ggcgtcgcgc tgcaggcgtg
      181 actggacggc gaaatcgccg tcgatgagct ggcgcaccag gccgtcgagg cggctgcgga
      241 tctgctcggc gcccagcact tcggtggcga cggcggccgg ttcggtattc gccgcgaggc
      301 gggcgagcgc gtcgtgcagc acgtggtagg cgccgtcgag gacatcgatc aggtcgagca
      361 cttcgttggc cgggcgctgc tggcggatgg cgtggtcgac ggcggcggcg gcttcgaaca
      421 cccgggtcgc gccgagcgtg ccggcggcgc ccttgatcga gtgggcgagc cggcgggctt
      481 cttcttcctc gccacagagc agcagctcgc ggattttttc gaagtcgtcg aggtgactgc
      541 cggcaaaggt gttgagcagg cgctcgtaga ccgcctgctt gccgcgcacg gcgtgcaggc
      601 ctagcttgta gtcgaggccc gggatgcgct cgagcgggtc gccggcagcg gccgggtcgg
      661 catccggcgg gcgcgtggcg aggtcgggct gacggctgtc tacggcccgc agcggcaggg
      721 tgaaccagaa ttcgctaccg aggccggggg cactgttgac gccgatttcg ccaccgagca
      781 gttcgaccag gcggcggctg atcaccaggc caaggccgac cccgccgtgg cggcgggtgg
      841 tcgaatcgtc accctgctcg aaggggtgga agaggcgggc gctgacctcg tcggcgatgc
      901 cgatgccggt atcgctgacc gagaagcgca gcagcagatg ctcgccgtcg cggtccgggt
      961 tgctgacggc gatggtgatg cggccggagt cggtgaactt gacggcattc gacagtagct
     1021 gctgcaggat ctggcggatg tgcggcgcgt cgccgcgcag cttttccgga atgccggcgg
     1081 cgaactggcg atcgatgatg atgttcttgc tgccggcttt ttcggcaaac tgttgactga
     1141 cctcggcgat cagttcgggg agcgggaact cgttgtcggc cggcgtcagg ttgccggctt
     1201 ccagtcgcga caggtcgagg atgtcgttga acagctgcat caactggttg gcggcgtcgg
     1261 caatgcgggc cagacgctcg ctgacgacgc tatcgccgat gtcgcgctgg agcaggtggg
     1321 tgaggccgag gatggcgttc atcggggtgc ggatttcatg ccccatgttg gccaggaagc
     1381 ggctcttcga gcggctggcc gcttcggcat cctccttcgc ctgtagcaac tgacgggtac
     1441 gctgggcgac cagcgattcg agctgttcgc ggtagcgggc gagttcggcc aggttgcgct
     1501 tgcgctcggt gatgtcttcg atgatcgcca ggtagtgggt gacttcgcta tccgcctgac
     1561 gaaccggcgt gatgatggcg cggatgtcgt agaaataacc gtcgcggcgt cggctcagca
     1621 gttcgccgcg ccagatttcg ccgcgccgga tgcaggcgtc gatttcggcg aagacgttct
     1681 gcgcggtcag gccttcggcc aggatggccg gtggtttgcc gatcacctcg gcggccgggt
     1741 agccggtgat ggcgatgaag gcctggttga cgtactcgat gccgtgctcg cggttggtga
     1801 tgatgacgcc gtgtggactc tgctcgatgg ccagcgacaa ctggcgcagg ctggcctcgc
     1861 tgctggcgag ccgttgccgc gcccgcatct tgccgatgcc gagaccgagt tcgacgccaa
     1921 tgtcttcgcg cagcagcctt tcttcgtcgt cgtcgctgac gtccggcgcc agtgccaggt
     1981 ggagccagcc gcagaggaca gcatcgtgct cgatggcgag ggagatttcg ccactgccga
     2041 cggccggcgg cctggtatcg gcggggtgga aactacagc
//
LOCUS       DAOSDX010000546         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18463_length_2078_cov_3.013347, whole genome shotgun sequence.
ACCESSION   DAOSDX010000546 DAOSDX010000000
VERSION     DAOSDX010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18463_length_2078_cov_3.013347"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(12..106)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            254..505
                     /locus_tag="PLN02_11320"
     CDS             254..505
                     /locus_tag="PLN02_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOI52960.1"
                     /translation="MQTYTANEAKTRFGEFLDRVQREPVRVMRHDRVVGVMVSAEDYE
                     AMRTFYADRLLKTMDETAAGAERAGLTPEKLADLLADES"
     gene            513..965
                     /locus_tag="PLN02_11325"
     CDS             513..965
                     /locus_tag="PLN02_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOI52961.1"
                     /translation="MRPASAGFAVVIDTNVWISGLLSASGAPAQLTRQAVRSGRPVFS
                     AETFAELRERLWRPKFDRYITLEQRKRFLLDLESIACWVDVPPTIAGQPLCRDPVDDK
                     FLHLALAAGANWLITGDQDLLVLADSAAAQRVGILSPAEALRLPPFSV"
     gene            1123..1893
                     /locus_tag="PLN02_11330"
     CDS             1123..1893
                     /locus_tag="PLN02_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3348 domain-containing protein"
                     /protein_id="HOI52962.1"
                     /translation="MARGLQHATFNRSALVRVLSGALPEVPALNYDFGERLGQWLDVS
                     EALTLYSVLAPRAGGGVAGGSANDDLPEQLARVRRHLSDSIKADGVFNAPPARIPFPL
                     PLPNATPESAADFSPYHRYYLAHQRDMNAAITALRASARKALAARSAAGRQLAELDAT
                     FDQSLAARERTLFGNIPILLSKRFQQRYADHRATLGEGGIDDPAAWAQPGSWLEAFCQ
                     DAQTVLLAELELRLKPVAGLIAALAAPPGSANHETNPR"
     gene            1890..>2078
                     /locus_tag="PLN02_11335"
     CDS             1890..>2078
                     /locus_tag="PLN02_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52963.1"
                     /translation="MNRNLSLFAFTLGLLAVVWVAIGYLDGHALAFSVSCIIAVVYVA
                     GALEMRRFHANTAALAAAL"
BASE COUNT          350 a          708 c          638 g          382 t
ORIGIN      
        1 ccagccggcg aatcatgtct gcagttcggc atgcctgagc gattatggga gtttgcgcct
       61 tcggcggagc ttggcgaacc agcgctctct ctcctgcaga ggggcaggat tatcggcagc
      121 gctggggatg cctgtcaatc tcgagcagcc ggccgagcgc tgacacagtg acgccgcctg
      181 aatcaggcgg tttgaccgaa tcgaacgatt taactacatt aagactgtca tagcagcacc
      241 gtgaggactg agcatgcaaa cctataccgc caatgaagcc aagacccgtt ttggcgaatt
      301 tctcgaccgt gtgcagcgcg agccggtgcg ggtgatgcgc catgaccggg tggtgggcgt
      361 catggtgagc gccgaggatt acgaggcgat gcgtaccttc tatgcggatc gtctgctgaa
      421 aaccatggat gaaactgccg caggcgcaga gcgcgccggg ctgaccccgg aaaagctggc
      481 cgacctgctc gccgacgaga gctgaacgga gcatgcgtcc ggcttctgcg ggtttcgcgg
      541 tggtcatcga taccaatgtc tggatcagcg gtttgctctc cgccagcggc gcgcccgcgc
      601 agttgacgcg tcaggcggtg cgaagcggcc gaccggtgtt ttcggccgag acctttgccg
      661 aactcaggga gcgcttatgg cggccaaaat ttgaccgcta catcacgctc gaacagcgca
      721 aacgcttcct gcttgatctt gaatccattg cttgctgggt tgatgtgccg ccgacgattg
      781 ccggccagcc actgtgccgc gacccggtgg atgacaagtt tctccatctg gcgctcgcgg
      841 ccggcgccaa ctggctgatc accggcgatc aggatttgct ggtcctggct gacagcgccg
      901 ccgcccagag ggtaggcatc ctctcgccgg ccgaagcctt gcgtctccca ccgttttccg
      961 tctgaaacga gcacgaacga aaccgaagcg cttcctgctc gcccatgctc tgtggctgac
     1021 cggcaagacg catgtcggca aacaagggaa gcgatcccgc tgaccccggc gcaaagttgt
     1081 agtagccttg cgcccccttt ttggtcgacc caggacatga tcatggcgcg tggattgcag
     1141 cacgccacat tcaaccgctc agcgctcgtc cgcgttctct ccggcgccct gccagaagtc
     1201 cccgcactga attacgattt cggcgagcgg ctcgggcaat ggctggacgt ttccgaagcg
     1261 ttaacgctgt attcggtcct tgctccccgg gccggcggcg gggtggccgg gggttcggca
     1321 aatgacgatc tccccgagca actggcccgc gtgcgccgcc atctgagcga ttcgatcaag
     1381 gccgacggcg ttttcaacgc gccgccggcg cgcattccgt ttccgctgcc gctgcccaat
     1441 gcgacgccgg aaagtgccgc cgacttttcg ccctaccacc gctattacct cgcccaccag
     1501 cgcgacatga acgccgccat caccgccctg cgcgccagtg cccgcaaggc gctggccgct
     1561 cggtcggcgg ccggtcggca gctggccgaa ctcgacgcca ccttcgacca gtcgctggct
     1621 gcccgcgagc gcacgctgtt cggcaacatc ccgatcctgc tcagcaagcg cttccagcag
     1681 cgttacgccg accaccgcgc cacgctcggc gagggcggca tcgacgatcc cgccgcctgg
     1741 gcacagccgg gcagctggct cgaagccttc tgccaggatg cccagaccgt gctgctggcc
     1801 gagctggaac tgcgcctgaa gcccgtcgcc gggctgatcg ccgccctggc tgccccgccg
     1861 ggcagcgcca accacgaaac gaatccccga tgaaccgcaa tctcagtctc ttcgccttca
     1921 ccctcggcct gcttgctgtc gtctgggtcg ccatcggcta cctcgacggc cacgcgctgg
     1981 ccttctccgt ctcctgcatc atcgccgtgg tctatgtcgc cggcgccctg gaaatgcggc
     2041 gcttccacgc aaatacggcg gcgctggcgg cggcgctg
//
LOCUS       DAOSDX010000547         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18503_length_2073_cov_3.549554, whole genome shotgun sequence.
ACCESSION   DAOSDX010000547 DAOSDX010000000
VERSION     DAOSDX010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18503_length_2073_cov_3.549554"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PLN02_11340"
     CDS             <1..1066
                     /locus_tag="PLN02_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI52964.1"
                     /translation="VARQVRAAFAATDDIVAIDDYIDADARKFVLHVLQAKAAQLGVA
                     QRDIVDVVGMGLSGLDVTPVRNTESKFAVPVRIVLPPEQLSSLDSLLKLRVRSREGRL
                     VPVSELVEVRELAREKTIYHKDLLPVVYVVGDMGGKLDSPLYGMFDIRSRINGMALKE
                     GGTLGEWFIRQPSDPYAGYSVKWDGEWQVTYETFRDMGLAYAVGLILIYLLVVAHFGS
                     YLVPLIIMAPIPLTIIGVMPGHALFGAQFTATSMIGMIALAGIIVRNSILLVDFIKLQ
                     VAAGMDFHDAVIQSAAVRAKPIALTALAAMLGAMFILDDPIFNGLALALIFGILVSTL
                     LTLVVIPVLYFAAFRKEHHA"
     gene            1063..1287
                     /locus_tag="PLN02_11345"
     CDS             1063..1287
                     /locus_tag="PLN02_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOI52965.1"
                     /translation="MTTERYIRIMAGFFVMLSLALGWQGSPIFVSEWFLAFTAFVGLN
                     LFQSGFTGFCPPEKLLRKLGVKSGGSGCGC"
     gene            complement(1293..1508)
                     /locus_tag="PLN02_11350"
     CDS             complement(1293..1508)
                     /locus_tag="PLN02_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOI52966.1"
                     /translation="MHRQSFKVAGLRDAQCVRALANAIQDLPGIEHLEIEPASGITDV
                     EHGNFVCPEEILQAITDAGFSAEYTTH"
     gene            complement(1566..2045)
                     /locus_tag="PLN02_11355"
     CDS             complement(1566..2045)
                     /locus_tag="PLN02_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HOI52967.1"
                     /translation="MFNSRWLLAPFYIGLILAIGILLIKFVKEFFKLAPQVFSADGSD
                     IIIGILTLIDVVMIANLLIIIVFAGYENFVSKINTGDNEDRPNWMGHVGFADLKMKLI
                     GSIVAISGIELLKAFMHVGSHSNEQLAWKVGIHMTFVISGVLYAVMDKLGGKHHAEE"
BASE COUNT          341 a          670 c          644 g          418 t
ORIGIN      
        1 cgtcgcccgc caggtgcgcg cggctttcgc ggcgactgac gacatcgtcg ccatcgacga
       61 ctacatcgac gccgacgccc gcaagttcgt cctccacgtg ctgcaggcga aggcggcgca
      121 gctcggcgtc gcccagcgcg acatcgtcga cgtcgtcggc atggggctgt ccggcctcga
      181 cgtgacgccg gtgcgcaaca ccgaatcgaa gttcgcggtg ccggtgcgca tcgtcctgcc
      241 gccggaacaa ctgtcctcgc tcgacagcct gctcaagctc cgcgtccgtt cgcgtgaagg
      301 gcgcctggtg ccggtctcgg aactggtcga agtgcgcgag ctggcgcgcg agaagacgat
      361 ctaccacaag gatctgctgc cggtcgtcta tgtcgtcggc gacatgggcg gcaagctcga
      421 ttcgccgctc tacggcatgt tcgacatccg cagcaggatc aacggcatgg cactgaagga
      481 aggcggcacg ctcggcgaat ggttcatccg ccagccgagc gatccctacg ccggctacag
      541 cgtcaagtgg gacggcgaat ggcaggtgac ctacgaaacc ttccgcgaca tggggctggc
      601 ctacgcggtc ggcctgatcc tgatctacct gctggtcgtc gcccacttcg gttcgtatct
      661 ggtgccgctc atcatcatgg cgccgatccc gctcaccatc atcggcgtca tgcccggtca
      721 cgcgctgttc ggcgctcagt tcacggcgac ctcgatgatc ggcatgatcg cgctggccgg
      781 catcatcgtc cgtaattcga tcctgctggt cgatttcatc aagctgcagg tcgccgccgg
      841 catggatttc cacgacgcgg tgatccagtc ggcggcggta cgggccaagc cgatcgccct
      901 caccgcgctg gccgccatgc tcggcgccat gttcatcctc gacgacccga tcttcaacgg
      961 cctggcgctg gcgctgatct tcggcatcct ggtgtccacg ctgctgaccc tggtcgtcat
     1021 tcccgtcctc tacttcgccg ctttccgcaa ggagcaccac gcatgaccac cgaacgctac
     1081 atccgcatca tggccggctt tttcgtcatg ctgtcgctcg ccctcggctg gcagggatcg
     1141 ccgatcttcg tctcggaatg gttcctcgcc ttcaccgcct ttgtcggcct caacctgttc
     1201 cagagcggct tcaccggctt ctgtccgccg gaaaagctgc tcaggaagct cggcgtcaaa
     1261 tccggcggca gcggctgcgg ctgctgagcc gttcagtgcg tcgtatattc ggcgctgaag
     1321 ccggcgtcgg tgatcgcctg caggatttct tccgggcaga cgaaattgcc gtgctcgaca
     1381 tcggtgatgc cgcttgccgg ctcgatctcg agatgctcga tacccggcag gtcctgaatc
     1441 gcgttggcca gcgcccgcac gcattgcgcg tcgcgcaggc cggcgacctt gaatgactgt
     1501 ctgtgcatga tgccctccgc gctgcctgtg gggatgtccc ttaggcagcg ccgctggcag
     1561 caagttcact cctcggcgtg gtgcttgccg ccgagcttgt ccatgaccgc gtagagcacg
     1621 ccggaaatga cgaaagtcat gtggatgccg accttccagg ccagttgttc gttgctgtgc
     1681 gagccgacgt gcatgaaggc cttgagcagt tcgatcccgg aaatggcgac gatcgagccg
     1741 atcagcttca tcttcaggtc ggcgaagccg acatggccca tccagttcgg gcggtcctcg
     1801 ttgtcgccgg tgttgatctt cgagacgaag ttctcgtagc cggcgaagac gatgatgatc
     1861 agcaggttgg cgatcatcac gacgtcgatc agcgtcagga tgccgatgat gatgtcgctg
     1921 ccgtcggcgc tgaagacctg cggcgcgagc ttgaagaact ccttgacgaa cttgatcagc
     1981 aggatgccga tggcgaggat cagcccgata tagaaggggg cgagcagcca gcggctgttg
     2041 aacatcagga cttcgagctt gtgttcgagc gat
//
LOCUS       DAOSDX010000548         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18564_length_2068_cov_3.305514, whole genome shotgun sequence.
ACCESSION   DAOSDX010000548 DAOSDX010000000
VERSION     DAOSDX010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18564_length_2068_cov_3.305514"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLN02_11360"
     CDS             complement(<1..216)
                     /locus_tag="PLN02_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOI52968.1"
                     /translation="MAFFSSSTRDLPQSTVVPQHLAVIMDGNGRWAKKRFLPRVAGHV
                     KGVDALRDMVRVCLDRGVRYLTVFAFSS"
     gene            complement(229..786)
                     /gene="frr"
                     /locus_tag="PLN02_11365"
     CDS             complement(229..786)
                     /gene="frr"
                     /locus_tag="PLN02_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOI52969.1"
                     /translation="MIAELKKTAEAKMQKSLEALKNDLLKIRTGRAHTGLLDHVMVDY
                     YGSPVPVNQVANITLVDARTLGVQPWEKNMLQKVEKAIRDSDLGLNPASQGELIRVPM
                     PALTEERRKEMIKVVKHEGEGAKVAIRNLRRDANTHLKDALKKGEIPEDAERKAQDDV
                     QKLTDKYIAEVDKMLAVKEAELMQV"
     gene            complement(798..1514)
                     /gene="pyrH"
                     /locus_tag="PLN02_11370"
     CDS             complement(798..1514)
                     /gene="pyrH"
                     /locus_tag="PLN02_11370"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOI52970.1"
                     /translation="MTTPKYKRILLKLSGEALMGSDAYGINPKVIGEICGEIKAVADL
                     GVEIGVVIGGGNIFRGMAGTATGMDRATADYMGMLATVMNAMALSDAMRQVGLNARVQ
                     SALNIEQVVEPYIRGKAIRYLEEGKVVIFGAGTGNPFFTTDTAAALRGSEIGAEIVLK
                     ATKVDGIYTADPKKDASATRYDKISFNDAISQNLAVMDATAFALCRDQKLPINVFSIF
                     KAGALKRVVCGEDEGTLVYC"
     gene            complement(1535..>2068)
                     /gene="tsf"
                     /locus_tag="PLN02_11375"
     CDS             complement(1535..>2068)
                     /gene="tsf"
                     /locus_tag="PLN02_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOI52971.1"
                     /translation="VESTRAALVGKIGENMTIRRFVRCAAQGKLVSYIHGGAKVGVLV
                     DLVGGDEQLGKDIAMHIAASKPKSLDASGVSAELLETERRVAIEKAREAGKPEAMLEK
                     IAEGTVQKYLKDVTLLGQVFVKAEDGKQTIEQLLKQKGASVASFTLFMVGEGIEKKVD
                     DFAAEVAAQAAAAAAKK"
BASE COUNT          348 a          659 c          619 g          442 t
ORIGIN      
        1 cgaagagaag gcgaacaccg tcaggtaacg aaccccccgg tcgagacaga cgcgaaccat
       61 gtcgcgcagg gcatcgaccc ccttgacatg acctgcgacg cggggcaaaa aacgcttttt
      121 tgcccagcgc ccgttaccat ccatgatcac cgcaaggtgt tgcggaacga cggtgctttg
      181 cggcaggtcc cgcgtcgaac tggaaaagaa cgccatcccg ggcgcggctc agacctgcat
      241 cagctcggcc tctttgacgg cgagcatctt gtcgacttcg gcgatgtact tgtcggtcag
      301 cttctggaca tcgtcctgcg ccttgcgttc ggcgtcctcg gggatttccc ccttcttcag
      361 ggcgtccttg aggtgggtat tggcatcgcg acgcaggttg cggatcgcca ccttggcgcc
      421 ctcgccttcg tgcttgacga ccttgatcat ttccttgcgc cgctcttcgg tcagcgccgg
      481 catcggcacg cggatcaact cgccctgcga ggccggattg aggccgaggt cggaatcgcg
      541 gatcgccttc tcgaccttct gcagcatgtt cttttcccag ggctggacgc cgagggtacg
      601 ggcatcgacc agagtgatgt tggcaacctg attcaccggc accggcgaac cgtagtaatc
      661 gaccatgacg tggtcgagca ggccggtatg ggcacggccg gtacgaatct tcagcaggtc
      721 gttcttgagc gcttcgagcg acttctgcat tttcgcttcg gcggttttct tgagttcggc
      781 aatcatctgt ctctccctca gcagtaaacc agcgtgccct catcctcacc gcagaccacg
      841 cgcttgagcg cgccggcctt gaagatggag aacacgttga tcggcagttt ctggtcgcgg
      901 catagtgcga aggcagtcgc atccatcacc gcgaggtttt gcgagatcgc atcgttgaag
      961 ctgatcttgt cgtagcgcgt ggccgaggcg tcctttttcg ggtcggcggt gtagatgccg
     1021 tcgaccttgg tcgccttgag cacgatttcc gcgccaattt ccgacccgcg caaggcggcg
     1081 gcggtgtcgg tcgtgaagaa ggggttgccg gtgcccgcac cgaaaatcac caccttgcct
     1141 tcttcgaggt agcggatggc cttgccgcgg atatagggct cgaccacctg ctcgatattc
     1201 agggccgact gcacacgcgc attcaggccg acttgacgca tcgcgtcgga cagcgccatg
     1261 gcgttcatca ccgtggccag catgcccatg taatccgcag tggctctatc catcccggtg
     1321 gccgtaccgg ccatgccgcg gaagatgttg ccaccgccga tcacgacgcc gatttccaca
     1381 cccaggtcag cgaccgcctt gatctcgccg cagatttcgc cgatcacctt cggattgata
     1441 ccgtaggcgt cgctgcccat cagggcctca ccggagagtt tgaggaggat gcgcttgtat
     1501 ttgggtgtgg tcatcggctt tcccttgcgg aagattactt cttggcagcc gcggcagcgg
     1561 cctgggcggc gacttcggca gcgaagtcgt cgaccttctt ctcgatgcct tcgccaacca
     1621 tgaacagcgt gaagctggcc accgaagcgc ccttctgctt cagcaactgc tcgatcgtct
     1681 gcttgccgtc ttcggccttg acgaagacct gaccgagcaa ggttacgtcc ttcaggtact
     1741 tctggaccgt accttcggcg atcttttcga gcatcgcttc cggcttgccg gcttcgcgcg
     1801 ccttctcgat ggcgacgcgg cgctcggttt ccagcagctc ggcggaaacg ccggaggcat
     1861 cgagagactt cggcttggac gcggcgatgt gcatcgcgat gtccttgccc agctgctcgt
     1921 caccgccgac caggtccacc agcacaccga ccttggcgcc gccgtggatg tacgaaacca
     1981 gcttgccttg cgcggcgcag cggacgaaac ggcggatcgt catgttctcg ccgatcttgc
     2041 cgaccagcgc agcgcgggtc gattcgac
//
LOCUS       DAOSDX010000549         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18598_length_2065_cov_3.709950, whole genome shotgun sequence.
ACCESSION   DAOSDX010000549 DAOSDX010000000
VERSION     DAOSDX010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18598_length_2065_cov_3.709950"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PLN02_11380"
     CDS             complement(<1..1368)
                     /locus_tag="PLN02_11380"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOI52972.1"
                     /translation="MHDIIRQLEKKRELARLGGGQKRIDSQHKKGKLTARERIELLLD
                     PESFEEWDMFKEHRCVDFGMDQAEKTPGDGVVVGYGTINGRLVFVFSQDFTVFGGSLS
                     ETHAEKICKVMDQAMKVGAPVIGLNDSGGARIQEGVASLGGYADVFQRNVMASGVVPQ
                     ISMIMGPCAGGAVYSPSMTDFIFMVKDSSYMFVTGPEVVKTVTHEDVTAEELGGAVTH
                     TSKSGVADLAFENDVEALSMLRRFMNFIPANNKEKPPVTPTNDPVDRFDYSLDTLVPD
                     NANKPYDMKELIIKVVDDNDFFELQADYAKNIIIGFGRMDGHPVGIVANQPLVLAGCL
                     DIKSSIKAARFVRFCDAFNIPVVTFVDVPGFMPGTQQEYGGIIKHGAKLLYAYAECTV
                     PKVTIITRKAYGGAYDVMSSKHLRGDVNLAWPSAEIAVMGPKGAVEIIFREEKKDPAK
                     LAAREA"
     gene            complement(1397..>2065)
                     /locus_tag="PLN02_11385"
     CDS             complement(1397..>2065)
                     /locus_tag="PLN02_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOI52973.1"
                     /translation="KTRMEQLSQREEAFIRPSPSAGSLGGVAEKTREAMLLCEAAGYD
                     VIIIETVGVGQSETTVAGMVDMFCLLQLPNAGDDLQAIKKGIVEIADMVVINKADIDK
                     QATAVVRAQWRNALHMLRPASPNWAPPVIALSALHKQGIADFWSEVEKYQAALKPTGE
                     FAAKRQHQSLAWMWQLIDAGLRQHFRLHPRVQENLPALTRSVEAGYTTPAAAAYALLD
                     YLKH"
BASE COUNT          351 a          613 c          689 g          412 t
ORIGIN      
        1 ggcttcgcgg gcggccagct tggccgggtc cttcttttcc tcgcggaaga tgatctcgac
       61 cgcgcccttc ggacccatga ccgcgatttc ggccgacggc caggcgaggt tgacgtcgcc
      121 gcgcaggtgc ttggacgaca tcacgtcgta ggcgccgccg taggccttgc gggtgatgat
      181 ggtgaccttc ggcacggtgc attcggcgta ggcgtagagc agcttggcgc cgtgcttgat
      241 gatgccgccg tattcctgtt gcgtgcccgg catgaagccc gggacgtcga cgaaggtgac
      301 gaccggaatg ttgaaggcgt cgcagaagcg gacgaagcgg gcagccttga tcgacgactt
      361 gatgtcgagg cagccggcca ggaccagcgg ctggttggcg acgatgccga ccgggtggcc
      421 gtccatgcgg ccgaaaccga tgatgatgtt cttggcgtaa tcggcctgca gttcgaagaa
      481 atcgttgtca tcgacgacct tgatgatcaa ttccttcatg tcgtacggct tgttcgcgtt
      541 gtccggcacc agggtgtcga gcgaatagtc gaaacggtcg accgggtcgt tggtcggcgt
      601 gaccggcggc ttttccttgt tgttggccgg gatgaagttc atgaagcggc gcagcatgga
      661 gagcgcttcg acatcgtttt cgaaggccag gtcggccaca ccggacttgc tggtgtgggt
      721 cacggcaccg cccagttctt cggcggtgac gtcttcgtgg gtgacggtct tgaccacttc
      781 cggaccggtc acgaacatgt aggacgagtc cttgaccatg aagatgaagt cggtcatcga
      841 tggcgagtac accgcgccgc cggcgcaggg gcccatgatc atcgagatct gcggcacgac
      901 gccggaagcc atcacgttgc gctggaagac gtcggcgtag ccgccgagcg aggcgacgcc
      961 ttcctggatg cgggcgccgc ccgagtcatt gaggccgatc accggggcgc cgaccttcat
     1021 cgcctggtcc atgaccttgc agatcttctc ggcgtgggtt tccgacagcg agccgccgaa
     1081 gacggtgaag tcctgcgaga agacgaagac gagacggccg ttgatcgtgc cgtagccgac
     1141 gacgacgccg tcgcccgggg tcttttcggc ctgatccatg ccgaagtcga cgcagcggtg
     1201 ttccttgaac atgtcccatt cttcgaagga ctccgggtcg agcaacagtt cgatgcgttc
     1261 gcgggcggtc aacttgccct tcttgtgctg gctgtcgata cgcttctggc caccgccgag
     1321 gcgggccagt tcacgcttct tctcaagctg gcggatgatg tcatgcatgg aaaaactccc
     1381 tcaatggaac gggtgatcag tgtttcagat agtcgagcag cgcatacgcg gcggcggccg
     1441 gggtcgtata tcctgcttcg acggaacggg tcagcgcggg caggttttcc tgaacgcgcg
     1501 gatgaaggcg gaaatgctgg cgcaagccgg cgtcgatcag ttgccacatc caggccagcg
     1561 actggtgctg gcgcttggcg gcgaactcgc cggtcggctt gagcgccgcc tggtatttct
     1621 cgacttcgct ccagaaatcg gcgatgccct gcttgtgcag ggcagatagc gcgatcaccg
     1681 gtggtgccca gttgggcgag gccggacgca gcatgtgcaa cgcattgcgc cactgggcac
     1741 ggacaacggc ggtagcctgc ttgtcgatgt cggccttgtt gatgaccacc atgtcggcga
     1801 tctcgacgat gcccttcttg atcgcctgca ggtcgtcgcc ggcattcggc aactgcagca
     1861 ggcaaaacat gtcgaccatg ccggcgaccg tcgtttccga ctggccaacc cccacggtct
     1921 cgataatgat tacgtcgtag cccgccgcct cgcagagcag catggcttcg cgcgtcttct
     1981 cggcgacgcc gccgagcgaa ccggccgacg gactgggccg gatgaaggct tcctcgcgct
     2041 gcgagagctg ctccatgcgc gtctt
//
LOCUS       DAOSDX010000550         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18610_length_2064_cov_3.021901, whole genome shotgun sequence.
ACCESSION   DAOSDX010000550 DAOSDX010000000
VERSION     DAOSDX010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18610_length_2064_cov_3.021901"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLN02_11390"
     CDS             <1..311
                     /locus_tag="PLN02_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52974.1"
                     /translation="RPALLRQNARQFLAGVHAQGFATGACAGQAVVPLILGDSGTVLA
                     FAEHLAGQGIQALPLVSPAVDECQARLVFCIHHGHGAESLERALAALGSARLRVGVKP
                     "
     gene            422..1825
                     /locus_tag="PLN02_11395"
     CDS             422..1825
                     /locus_tag="PLN02_11395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI52975.1"
                     /translation="MNDYTLLHHGAVDGVTGSCHELRARDGTAILVDCGLFQGAEVSA
                     EGGSAGTPAIDFPVDHIRAIVITHVHIDHVGRLPYLLAAGFRGPILCSEASAELLPLM
                     LEDAMKVGMTPDQRLIRRILEQIAGQLVALPYKHWHALLPGWQIKLHPAGHILGSAFV
                     EVRLSGELATGSDKGDWRIVFSGDLGAPHSALLPAPRSPWRADLLVLESTYGDRLHVD
                     RKQRRRQLQETIERALRNRGSVLIPAFSIGRTQELLYDLEHVIASAKKQPAETDWSAL
                     EIIVDSPLAARLTQAHIRLHHLWDAEARRRTASGRHPLAFEQLYTVDGHDEHLQTVAY
                     LARSARPAVVIAAGGMCSGGRIVNYLKAMIGDARHDIVFVGYQAAGTPGHAIQQYGPR
                     GGYVELDGERHSIRAGIHRIAAYSAHADQADLLRFVGRMRHQPAEIRLVHGDRRAKEV
                     LMEKLHARFPETQVRIP"
     gene            1884..>2064
                     /locus_tag="PLN02_11400"
     CDS             1884..>2064
                     /locus_tag="PLN02_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52976.1"
                     /translation="MQMTVINRIGRGATVIAFLAICWLSVMPSEIAAVDGFNDKAKHV
                     FAFLVLAVGLSTFWRS"
BASE COUNT          347 a          687 c          647 g          383 t
ORIGIN      
        1 cgcgcccggc gttgctgcgg caaaacgcca ggcagtttct ggccggcgtg catgcccagg
       61 gtttcgccac cggcgcctgt gccggacaag cggtggttcc gctcatcctc ggcgattccg
      121 ggacggtcct ggcgttcgcc gagcacttgg cgggccaggg aatccaggca ctgccgctgg
      181 tttccccggc ggtcgacgag tgccaggcac gcctggtctt ctgcattcac catgggcatg
      241 gcgctgagtc actggagcgg gcgctggcag cgctgggcag cgcccggctg cgggtaggcg
      301 tgaagccgta ggcagtactc cgtagtctgc gcttcgcccg agggcggcag caattcgcca
      361 gccgaatcgc gccgcatgaa aaatgcaaat atcattgccg ttttcttccc cggagaacgc
      421 aatgaacgat tacacgttgc tgcaccacgg tgccgtcgat ggcgtcaccg gttcctgcca
      481 cgaactgcgc gcccgggatg gcacggccat tctcgtcgac tgcgggctgt ttcagggggc
      541 cgaggtctcc gccgagggcg gttcggccgg cacgccggca atcgactttc ccgtagacca
      601 catccgggcc atcgtcatca cccatgtgca catcgatcat gtcggccgtc tgccctacct
      661 gctggcggcc ggatttcgcg gccccattct gtgcagcgag gcgagtgccg aactgctgcc
      721 gttgatgctg gaggatgcga tgaaggttgg catgacgcct gaccaacgcc tgatccggcg
      781 gatactggag caaatcgccg gccagttggt ggcgctgccc tacaagcact ggcatgcctt
      841 gcttcccggt tggcagatca agctgcaccc ggccgggcat attctcggtt cagccttcgt
      901 cgaagtccgc ctcagcggcg aactggcaac gggttccgac aaaggcgact ggcgtatcgt
      961 cttttccggc gatcttggcg ctccccacag cgcgcttctg ccggcacccc gttcgccatg
     1021 gcgggccgat ctgctggtcc tcgaaagcac ctacggcgat cgcttgcacg ttgatcggaa
     1081 gcagcgccgc cggcaactgc aggagaccat cgaacgcgcc ctgcgcaacc ggggcagcgt
     1141 cctcattccc gccttcagca tcgggcgtac ccaggaactc ctctacgacc tggagcatgt
     1201 catcgccagc gcgaaaaagc agccggcgga aaccgactgg tcggcgctgg agatcatcgt
     1261 cgattccccg ctggcggcgc gtctgacgca ggcacatatc cgcctgcatc acctgtggga
     1321 cgccgaagcc cgccgccgca ccgccagcgg tcgccacccc ctggccttcg aacagctcta
     1381 tacggtggac ggtcacgacg agcatctgca aaccgtcgcc tacctggcgc gcagcgcccg
     1441 cccggcggtc gtcatcgccg ctggcgggat gtgctccggc gggcgcatcg tcaattacct
     1501 gaaagccatg atcggcgacg cccggcacga catcgtcttc gtcggctatc aggcggccgg
     1561 aacgcccggg cacgccatcc agcaatacgg accgcgcggc ggctatgtcg aactcgacgg
     1621 tgaacgccac agcatccgcg ccggcattca ccgcatcgcg gcgtattccg cgcacgccga
     1681 ccaggccgac ctgctgcgct tcgtcgggcg tatgcgccac cagccggcgg aaatccgtct
     1741 ggtccatggt gatcgccggg ccaaggaggt cttgatggaa aaactgcacg caagatttcc
     1801 ggagacacag gttcgcattc cttagccgtc agttcgctgg gcgaaacggc tagaatgccc
     1861 gattcgcgtt cgggcagatt tgcatgcaga tgacagtcat caacaggata gggcgaggtg
     1921 ccaccgtcat tgcttttttg gcgatttgct ggctgtctgt catgccgagc gagatcgccg
     1981 cggtcgatgg cttcaacgac aaggccaaac atgtctttgc ctttctcgtt ctggccgtgg
     2041 gcctttccac gttctggcgg agtt
//
LOCUS       DAOSDX010000551         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18684_length_2058_cov_2.881677, whole genome shotgun sequence.
ACCESSION   DAOSDX010000551 DAOSDX010000000
VERSION     DAOSDX010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18684_length_2058_cov_2.881677"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLN02_11405"
     CDS             <1..476
                     /locus_tag="PLN02_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI52977.1"
                     /translation="RGGAIEIRHGHFGAGDPGFNGLLMKAIARVAQEDQAETLICPSG
                     APTQARYTILLEPSRHGERPLPGQARNVACLVFPLGRRRIASARQLMSMFGLSAAEAR
                     LARALCHGETLEEYAEAQGVKLPTVKTQLRAVFGKTQTDRQVTLVALISGIPPLR"
     gene            636..1607
                     /locus_tag="PLN02_11410"
     CDS             636..1607
                     /locus_tag="PLN02_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI52978.1"
                     /translation="MAASCSWESIDVHLLQVLHALLTECSVSNAARRLNQSQPAVSTA
                     LRRLRDITGDQLLVRSRNGMTPTERGQSLLEPVRIALAQIEAIGLQQVRFDPMKTKRV
                     FNIATPDYLNAILIGNIVAHLRQHAPHSQVVLHSFGPEHDYTKALETGELDIVIGNWP
                     QPPEHLRLAPLFEDDVVCLMRKRHPLAGHRLTADDYQNAEHLVPTPYAVGQRGVIDMQ
                     LARDRIKRNVVASVPYFNLVPYVLQQADLIFSAPRIFAEHCCALGDLCYAESPVEFPK
                     MQFYLLWHDRTHHAEECRWFREQIGEVIRRRINPEAADSATRQRLRA"
     gene            complement(1610..>2058)
                     /gene="uraD"
                     /locus_tag="PLN02_11415"
     CDS             complement(1610..>2058)
                     /gene="uraD"
                     /locus_tag="PLN02_11415"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HOI52979.1"
                     /translation="VFEHTPWVMARVWERRPFADRAALREAMQAVLAEAARAEMLALI
                     LAHPELASKAALRGELTADSAREQAGAGLDACTPEELTEIRRLNDAYRARFGWPFIVA
                     VRGLSRQDIIVAMRRRLQRSDDEEFAEARAQILRIAELRLDPLLAA"
BASE COUNT          348 a          713 c          675 g          322 t
ORIGIN      
        1 agcgcggcgg cgccatcgag attcggcatg gtcatttcgg ggcgggcgac cccgggttca
       61 atggcctgtt gatgaaggcg attgcgcggg tggcacagga ggatcaggcg gaaaccctga
      121 tttgcccgag cggcgcacca acgcaggcgc gctacacgat cctgctggag ccgagccgac
      181 acggcgagcg gcctttgccc gggcaggccc ggaacgtcgc ctgtctggtg ttcccgctcg
      241 gccgacggcg catcgccagc gcccgccagt tgatgtcgat gtttggcctg tcggcggccg
      301 aagcgcggct ggcgcgggct ttgtgtcatg gtgagacatt ggaggaatac gccgaggccc
      361 agggagtcaa gctgccgacc gtgaaaaccc agttgcgcgc tgtctttggc aagacccaga
      421 cggaccggca ggtcaccctg gttgccctga tttcgggaat cccgccgctg cgctgagccg
      481 ccagcctgcc gccatgcctt cacgcggtgc gcggcaagcg ccgaatgcgc gaaagcggtg
      541 ctccaaagtg ccgaaaatgg ccgttttacc ccatgttggc aaaggcgcgc ttattgctaa
      601 gagagcctga caacaatttc gactagggga aaagcatggc cgcgagttgt tcctgggaat
      661 ccatcgacgt gcatctgctg caggtgctgc atgccctgct caccgaatgc agcgtctcca
      721 acgccgcccg ccgcctcaac cagtcgcagc cggcggtgag caccgcgctg cgccggctgc
      781 gcgacatcac cggcgaccag ttgctggtgc gcagccgcaa cggcatgacg ccgaccgagc
      841 gcggccagtc gctgctcgaa ccggtgcgta tcgcgcttgc gcagatcgag gccatcggcc
      901 tgcagcaggt gcgtttcgac ccgatgaaga cgaagcgcgt gttcaacatc gccacgccgg
      961 attacctgaa cgccatcctg atcggcaaca tcgtcgccca cctgcgccag cacgcaccgc
     1021 attcgcaggt cgtgctgcat tccttcggcc ccgagcacga ctacaccaaa gcgctggaaa
     1081 ccggcgagct cgacatcgtc atcggcaact ggccgcagcc gcccgagcac ctgcgcctgg
     1141 cgccgctgtt cgaggacgac gtcgtctgcc tgatgcgcaa gcgtcatccg ctggccggcc
     1201 accggctcac cgccgacgac taccagaacg ccgaacacct ggtgccgacg ccctacgccg
     1261 tcggccagcg cggcgtcatc gacatgcaac tggcgcgcga ccggatcaag cgcaacgtcg
     1321 tcgccagcgt gccctacttc aatctggtgc cctacgtgct gcagcaggcc gacctgatct
     1381 tctcggcgcc gcgtatcttc gccgaacact gctgcgcact gggcgatctc tgctatgccg
     1441 aatcgccggt cgagtttccg aagatgcagt tctacctgct ctggcacgac cgcacgcacc
     1501 acgccgagga atgccgctgg ttccgcgagc agattggtga agtgatccgc cgccggatca
     1561 acccggaggc tgccgacagc gcaacgcgcc agcgcctgcg cgcctgagtt caggcggcca
     1621 gcagcgggtc gagacgcagc tcggcgatgc gcaggatctg cgcccgcgct tcggcgaatt
     1681 cctcgtcgtc gctgcgctgc aggcggcggc gcatggcgac gatgatgtcc tggcgactca
     1741 acccgcgcac ggcgacgatg aagggccagc cgaagcgggc gcgataggcg tcgttgaggc
     1801 ggcggatttc cgtcagttct tcgggagtac aggcgtcgag cccggcaccg gcctgctcgc
     1861 gcgccgaatc ggcggtcagc tcgccgcgca gcgccgcctt gctggccagc tccgggtggg
     1921 cgaggatcag ggcgagcatc tcggcgcgcg ccgcctcggc cagcaccgcc tgcatcgcct
     1981 cgcgcagcgc cgcgcggtcg gcgaaaggac ggcgctccca gacccgcgcc atgacccagg
     2041 gcgtgtgctc gaacaccg
//
LOCUS       DAOSDX010000552         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18726_length_2055_cov_3.084500, whole genome shotgun sequence.
ACCESSION   DAOSDX010000552 DAOSDX010000000
VERSION     DAOSDX010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18726_length_2055_cov_3.084500"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLN02_11420"
     CDS             <1..1215
                     /locus_tag="PLN02_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOI52980.1"
                     /translation="CIVEQAGADLVLSLDFAPGREYRFSAGSDILCRIHPDTHVTFDL
                     PADTALQLDVEAEVIEREDAPNRRTIILGDGSAHVHIGAANRLRLVGDARSVDQRLSA
                     VATRLSWFFSAMLGAIAVAWLVIEAGLIRRIARLTRRTRGLSRSVQADGGLERYELAD
                     LRGRDELGLLASGLDELLRRVREDAGRERIRAAQEKDMWHAVGHEIMSPLQSLLALHG
                     DPADPSHRYISRMQQAVRVLYGSASPSEAFAGSQLEIASLDLAAFLQHVAENAGIADL
                     SCAGVDAPVLVRADEFALEDVLAHLLRNAERYRAPGSPITLTLSADEHQASVSVANRG
                     PGIAEEMLERIFEYGVSDQADAGAAGNRGQGLFVARTYMAKMGGTIVARNSADGVAFV
                     LSLPRADFAPAR"
     gene            complement(1222..>2055)
                     /locus_tag="PLN02_11425"
     CDS             complement(1222..>2055)
                     /locus_tag="PLN02_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOI52981.1"
                     /translation="AVVFFNFRGDRALEITRAFVEADFDKFDRVRHPRVTYAGMLQYD
                     GDLHLPERFLVPPPAIRDTSGEWFSKAGITQFACSETQKFGHVTYFWNGNRSGKFDGE
                     TYLEVPSDVVPFEQRPWMKAAEIADAMVAAIASGQYKVLRCNFANGDMVGHTGNFRAA
                     TMAVEAVDLALGRVLKALEAAGGVALITADHGNADEMFELDKKSKQPARNADGSFKAK
                     TAHTLNPVPLILFNASGDARLGLRQTADAGLSNIAATVANLLGFDKHPAWDESLLTFG
                     E"
BASE COUNT          310 a          722 c          708 g          315 t
ORIGIN      
        1 tgtatcgtgg agcaggccgg cgccgacctg gtgctcagcc tggacttcgc gccgggccgt
       61 gagtaccgct tcagcgcagg gagcgacatc ctgtgccgca ttcaccccga cacccacgtg
      121 accttcgacc tgcccgccga tactgcgttg cagcttgacg tggaagccga agtcatcgag
      181 cgcgaagacg ctcccaaccg gcgcaccatc atcctgggcg acggcagcgc ccacgtgcac
      241 attggcgcgg ccaaccggct gcgcctggtc ggcgacgcgc gcagcgtgga ccagcggctg
      301 tcggcggtcg ccacccggct gtcctggttc ttcagcgcca tgctcggcgc catcgcagtc
      361 gcctggttgg tcatcgaggc cgggctgatc cgccgcatcg cccggctgac ccggcgcacc
      421 cgcggcctat cgcgctcggt gcaggccgac ggcgggctgg agcgctacga actggccgac
      481 ctgcgcggcc gcgacgaact cggcctgctc gccagcggcc tcgacgagct gctgcgccgg
      541 gtgcgcgagg acgccgggcg cgagcgcatt cgcgccgccc aggagaagga catgtggcac
      601 gccgtcggcc acgagatcat gtcgccactg cagagcctgc tggcgctgca cggcgacccg
      661 gccgacccca gccatcgcta catcagccgc atgcagcagg cggtgcgcgt gctctacggc
      721 agcgccagcc cgagcgaggc cttcgccggc agccagctgg aaatcgccag cctcgacctc
      781 gccgccttcc tgcagcacgt cgccgaaaac gccggcatcg ccgacctgag ctgcgccggc
      841 gtcgacgcgc cggtcctcgt gcgcgccgac gagttcgcgc tggaagacgt cctcgcccac
      901 ctgctgcgca acgccgaacg ctatcgtgcg ccgggttcgc cgatcaccct gacgctgagc
      961 gccgacgaac accaggccag cgtcagcgtc gccaaccgcg ggccgggcat tgccgaagaa
     1021 atgctggaac gcatcttcga gtacggcgtc tccgaccagg ccgacgccgg cgccgccggc
     1081 aaccgcggcc aggggctgtt cgtcgcccgt acctacatgg ccaagatggg cggcaccatc
     1141 gtcgcgcgca acagcgccga cggcgtcgcc ttcgtcctca gcctgccgcg cgccgacttc
     1201 gcgccggcgc gctgaacagc gtcactcgcc gaaggtcagc aggctttcgt cccaggccgg
     1261 gtgcttgtcg aaaccgagca ggttggcgac cgtcgccgcg atgttggaga ggccggcgtc
     1321 cgccgtctgg cgcagaccga ggcgggcatc gccgctggcg ttgaagagga tcagcggcac
     1381 cgggttcagg gtgtgcgcgg tcttcgcctt gaacgaaccg tcggcgttgc gtgccggctg
     1441 cttgctcttc ttgtcgagtt cgaacatttc gtcggcgttg ccgtggtcgg cggtgatcag
     1501 cgccacgccg ccggccgctt cgagcgcttt cagcacgcgg ccgagcgcca ggtcgacggc
     1561 ttcaacggcc atcgtcgcgg cgcggaagtt gccggtatgg ccgaccatgt cgccgttggc
     1621 gaagttgcag cgcagcacct tgtactggcc ggaggcgatc gccgccacca tggcgtcggc
     1681 gatttccgcc gccttcatcc acggccgctg ctcgaaggga acgacgtcgc tcggcacttc
     1741 gagataggtt tcgccgtcga acttgccgga gcggttgccg ttccagaaat aggtgacgtg
     1801 gccgaacttc tgcgtctcgg aacaggcgaa ctgggtgatg ccggccttgc tgaaccattc
     1861 gccggaagtg tcgcggatcg ccggcggcgg caccaggaag cgctcgggca ggtgcaggtc
     1921 gccgtcgtac tggagcatgc cggcgtaggt gacgcgcgga tggcggacgc ggtcgaactt
     1981 gtcgaaatcg gcctcgacga aggcgcgggt gatttccagc gcgcggtcgc cgcggaagtt
     2041 gaagaagacg acggc
//
LOCUS       DAOSDX010000553         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18744_length_2053_cov_3.017017, whole genome shotgun sequence.
ACCESSION   DAOSDX010000553 DAOSDX010000000
VERSION     DAOSDX010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18744_length_2053_cov_3.017017"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /gene="dnaG"
                     /locus_tag="PLN02_11430"
     CDS             complement(<1..717)
                     /gene="dnaG"
                     /locus_tag="PLN02_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOI52982.1"
                     /translation="MIPEDFIQDLLARVDIVDLVDSYVPLKKAGANYAACCPFHNEKT
                     PSFTVSPSKQFYHCFGCGAHGTAIGFLMEYQGLSFVEAVHELAKMVGMIVPQETRDSA
                     RPMVKPVPGLLETMQQAAVYYKGELKKSPRAIEYLKSRGLSGQIAARFQMGYAPQGWQ
                     NLQSVFPHYDDDVLQTAGLVVENEQGRRYDRFRDRIMFPIHDQKGQVIGFGGRVIDPE
                     DTPKYYNSPETPLFQKGHELY"
     gene            complement(728..>1118)
                     /locus_tag="PLN02_11435"
     CDS             complement(728..>1118)
                     /locus_tag="PLN02_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOI52983.1"
                     /translation="TARLAAIRLLLAAIKQKEVDERCELDDNATVAVIEKLVKQRKDS
                     VAQYQAAGRQDLADAEQAEITILGAYLPEKMGGAEVAAAVAAAVAETGAAGPADMGKL
                     MAVLKPRLAGKADMAEVSRLVKAALAG"
     assembly_gap    1119..1128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1242..1454)
                     /gene="rpsU"
                     /locus_tag="PLN02_11440"
     CDS             complement(1242..1454)
                     /gene="rpsU"
                     /locus_tag="PLN02_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOI52984.1"
                     /translation="MPSIRVKENEPFEVAIRRFKRTVEKTGLLTELRAREFYEKPTAE
                     RKRKAAAAVKRQNKRLRSLTLPPKMY"
     gene            1593..>2053
                     /locus_tag="PLN02_11445"
     CDS             1593..>2053
                     /locus_tag="PLN02_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451884.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOI52985.1"
                     /translation="MLILGVESSCDETGIALYDSAAGLVAHALHSQVAMHAEYGGVVP
                     ELASRDHIRRVVPLLRSVLAQSGKALADVDVIAYTRGPGLSGALLVGCAFAEALALAL
                     DKPTLPVHHLEGHLLSPLLSADPPTFPFVALLVSGGHTQLMRVSAVGDYELL"
BASE COUNT          323 a          650 c          640 g          430 t
ORIGIN      
        1 gtaaagttca tgacctttct gaaacagcgg ggtttccggc gagttgtaat acttcggcgt
       61 atcttcggga tcgatcaccc ggccgccgaa accgataacc tgccccttct ggtcatggat
      121 cgggaacatg atacggtcgc ggaaacggtc atagcgtcgc ccctgttcat tttcgaccac
      181 gagaccggcc gtctgcagca catcgtcgtc ataatgcggg aacacgctct gaaggttctg
      241 ccagccctgc ggcgcataac ccatctggaa tcgggctgcg atctggccgc tgagcccgcg
      301 gcttttcaga tattcgatcg cgcgcggcga tttcttcagt tcgcccttgt agtagaccgc
      361 agcctgctgc atggtttcca gcagaccggg gacaggcttt accatcggcc tggcgctatc
      421 gcgcgtttcc tgcggcacga tcatgccaac catcttcgcc agttcgtgca ccgcctcgac
      481 gaaactgagc ccctgatatt ccatcaggaa gccgatagcg gtgccgtggg cgccgcagcc
      541 gaagcagtgg tagaactgct tgctcgggct gaccgtgaac gacggtgtct tttcgttgtg
      601 gaaggggcag caggcagcgt aattggcgcc cgccttcttc agtggtacgt agctgtcgac
      661 caggtcgacg atgtcgaccc gggccagcag atcctggatg aagtcttccg ggatcatggt
      721 ctgcgcatca gccggcgagg gcggccttga ccagtcggga gacctcggcc atgtcggcct
      781 tgccggccag acgcggcttc agtacggcca tcagcttgcc catgtcggcc gggccggcgg
      841 cgccggtttc ggcgacggcg gcggcaaccg ccgcggcaac ttcggcgccg cccatcttct
      901 ccggcaggta ggcgccgagg atggtgatct ccgcctgttc ggcatcggcc agatcctggc
      961 ggccggcggc ctggtactgg gcgacgctgt ccttgcgctg cttgaccagt ttctcgatga
     1021 cggcgacggt ggcgttgtcg tcgagttcac agcgctcgtc gacttccttc tgcttgatcg
     1081 ccgccagcag caggcggatt gcggcgaggc gagcggttnn nnnnnnnngt cttcggtgat
     1141 gcgtgctttg agggtaatcg tcgttgctgt ggcggaaaaa ggacaaagcc gccggctttt
     1201 gagggcgggc ggcttggtgt ttgcagcgaa ggcgtactcg atcagtacat cttcggcggc
     1261 agggtcaggc tgcggagacg cttgttctga cgcttgacag cggcggcggc cttgcgcttg
     1321 cgttcggccg tgggcttctc gtagaactca cgggcgcgca gctcggtcag gagacccgtc
     1381 ttttcgaccg tgcgcttgaa gcggcgaatc gccacctcga acggctcgtt ttccttgaca
     1441 cgaatgctcg gcataaaaat cacccccttc cgtcaggcgc ccgccaggtc aagtaatcgg
     1501 cggtgcgcgc aaatttgtat ccgcggattg cggaaagctc gagattctat gccagatatc
     1561 ccagcctgat caagtgttaa aatgcgcctc ccatgctgat cctcggtgtt gaatcctcct
     1621 gcgacgaaac cggcatcgcg ctctacgaca gcgcggccgg cctcgtcgcc catgccctcc
     1681 actcacaggt agcgatgcac gccgaatacg gtggcgtcgt tcccgagctg gcttcgcgcg
     1741 accacatccg gcgcgtcgtg ccgttgttgc gctcggtgct ggcgcagtcg ggcaaggcgc
     1801 tggccgatgt tgatgtcatc gcctacaccc gcggtcccgg cctctccggc gcactgctcg
     1861 tcggttgcgc cttcgccgaa gcgttggcgc tggcgctcga caaaccgacg ctgccggtgc
     1921 atcacctcga aggccacctg ctgtcgccgc tgctgtccgc cgatccgccg acctttccct
     1981 tcgtcgccct gctcgtttcc ggcggccaca cccagttgat gcgggtgagc gccgtcggcg
     2041 actacgaact cct
//
LOCUS       DAOSDX010000554         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18753_length_2052_cov_3.556835, whole genome shotgun sequence.
ACCESSION   DAOSDX010000554 DAOSDX010000000
VERSION     DAOSDX010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18753_length_2052_cov_3.556835"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /gene="cas9"
                     /locus_tag="PLN02_11450"
     CDS             complement(<1..1743)
                     /gene="cas9"
                     /locus_tag="PLN02_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOI52986.1"
                     /translation="MSATYTIGLDIGIASVGWCVLGETHIIDLGVRAFDKAETAKEGE
                     SLNLARRNARLMRRRLFRRAWRLKKLARLLKSEGLIADAKAFQPEHAFTHSLWQLRVD
                     GLDRRLNDEEWARVIYHLSKHRGFHWISRAEAKQAEGDSKSEGGKVKQGLAGTAKLMT
                     EKGYRSAAEMVLGEFPDAQRNKQGEYTKALARELLAKELALLFEKQRELGNPHASSAL
                     QTAILGNGDKKSGLFWQQKPALAGADLLKMLGTCTFEKGEYRAPKASFTAERHIWLTR
                     LNNLRIVTDGVTRQLTEQERQLCLPLPYQQAGDLTYKQLGTALIKAGLLEAGSFKFTG
                     LAYPTEAQKAEGKAKDPESATLIKIPAWQEVRQTLTKAGLDDEWKKLSGDALCGQPEI
                     FDQIAWVLSVYKDDDEVRAELAKLSLPNHDALIDALLNIRFDKFHALSLKALRAIVPP
                     MEKGLRYDEACVEAGYHHSQLHKVGEGEHKYLPPLYAGRDKDGRMRFAEDADIPRNPV
                     VLRSLNQARKVVNAIIRRYGSPAAIHIEMARDLSRPLDERNKIKKEQDEYQARNQRDR
                     EQFIELFGHPPKGLE"
BASE COUNT          325 a          565 c          680 g          482 t
ORIGIN      
        1 ctccaggcct ttgggcggat ggccgaacaa ttcgatgaac tgttcccggt cgcgttgatt
       61 ccgggcctgg tattcgtcct gctccttctt gatcttgttg cgctcgtcga gggggcgcga
      121 gagatcgcgg gccatttcga tgtggatcgc agccggtgag ccgtagcggc ggatgatggc
      181 attgaccacc ttgcgcgcct gattcagcga acgcaggacg acaggattgc gcgggatgtc
      241 ggcatcttcg gcgaagcgca tgcggccgtc cttgtcgcgt ccggcgtaga gcggcggcag
      301 atatttgtgt tcgccttcac cgaccttgtg caactggctg tggtgatagc cggcctcgac
      361 gcaggcctcg tcgtagcgca gccccttttc catgggcggg acaatggcgc gcagggcttt
      421 cagcgacagg gcgtggaatt tgtcgaaacg gatgttgagc agggcgtcga tgagcgcgtc
      481 gtggttgggg agcgacagct tggcgagttc ggcgcggact tcgtcgtcgt ccttgtagac
      541 actgagcacc caggcaattt ggtcgaagat ttccggttga ccgcagagcg catcgcccga
      601 gagttttttc cactcgtcgt cgaggccggc tttggtcagc gtttggcgca cttcctgcca
      661 ggcggggatt ttgatcagcg tggcggattc ggggtctttc gccttgcctt ccgccttttg
      721 cgcttcggtg gggtaggcga gaccggtgaa tttgaagctg ccggcttcca gcagcccggc
      781 tttgatcagc gccgtaccga gttgcttgta ggtcaggtcg ccggcttgtt ggtaaggcaa
      841 gggcaggcag agttggcgtt cctgttcggt cagttggcgg gtgacgccat cggtgacgat
      901 gcgcagattg ttcaggcgcg tcagccagat gtggcgttcg gcggtgaaac tggctttcgg
      961 ggcgcggtat tcaccttttt cgaaggtgca ggtgccgagc atcttgagca ggtcggcccc
     1021 ggcgagggcc ggtttttgct gccagaacaa tccgctcttc ttgtcgccgt tgccgaggat
     1081 ggcggtttgc agcgcacttg atgcgtgggg attgccgagt tcccgttgtt tttcgaagag
     1141 cagcgccagt tccttggcga gcagttcccg cgccagagct ttggtgtatt cgccctgctt
     1201 gttgcgttgc gcatcaggaa attcgcccaa caccatttcg gccgcactgc gataaccttt
     1261 ttccgtcatc aatttggcgg tgccggcgag cccctgtttg accttgccgc cttcgctctt
     1321 gctgtcgccc tcggcttgct ttgcttcggc gcggctgatc cagtggaagc cccggtgttt
     1381 gctgagatgg tagatgacgc gtgcccattc ttcgtcgttc aggcggcggt cgaggccgtc
     1441 aacgcgcaat tgccagagcg aatgggtaaa agcgtgttcg ggctggaatg ccttggcgtc
     1501 ggcgatcaga ccttcacttt ttaacaggcg ggccagcttt ttcagccgcc atgctcgccg
     1561 gaacaagcgc cgccgcatca ggcgcgcatt gcgtcgggcg aggttcagcg attccccttc
     1621 cttggccgtt tcggccttgt cgaaggcgcg cacgcccagg tcgatgatgt gggtttcacc
     1681 aagaacgcac cagccgaccg atgcgatgcc aatgtccaga ccaatggtgt atgtcgcgct
     1741 catgatttgc tgttctctat ccggaatgcg tagaataacg ccatgtagct ttccggggtg
     1801 agagccgttg ctacaataag gtgtcacctt cgggtgacga ccgaatccgg cccggcaggg
     1861 aaacctgtcg ggccttctct ttgtgcgccc agcatgggcc attcgatcgg gtgcaagttc
     1921 cgccgtgagt cgatcaccgc gaacgcagtg aagtgcaact gcgtgagggc gaccgagcgt
     1981 ggatcgtatc cgcgagccga tggacgagaa ccggatagaa ggcgttgccg agcagggcga
     2041 gtggtcaatg ga
//
LOCUS       DAOSDX010000555         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18781_length_2050_cov_2.315288, whole genome shotgun sequence.
ACCESSION   DAOSDX010000555 DAOSDX010000000
VERSION     DAOSDX010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18781_length_2050_cov_2.315288"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLN02_11455"
     CDS             complement(<1..741)
                     /locus_tag="PLN02_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HOI52987.1"
                     /translation="MRAISCFLLALFASAGASAEPYAYVPNEKSASVSVIDTASDTVV
                     RQFPIGERPRGIAGGSGRLYLTDGKSNSLLIVDSAAGKLERRVKVGDSPEGVSLSPDG
                     KLLAVAVEDDNVVTLLSAPAAKELARIKVQGKNPEHAVFSPDSRWLYVSAEDAEQVDV
                     IDVAARRQVASIPVGKRPRGIGFLADGSRAYVACELADKVYAIDVASHQAIAEIPAGQ
                     YPNGVAIHPDGKRVYISNGRDGSVMAIDV"
     gene            complement(749..1594)
                     /locus_tag="PLN02_11460"
     CDS             complement(749..1594)
                     /locus_tag="PLN02_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOI52988.1"
                     /translation="MKNDRRRCLKALAALPFCLALPAVADALETVRQRGRLRIAVYND
                     FPPYSLAGGKGIDADLGRAIAARLGVAAEIVGFNADEDMNDDLRNMVWKGHYLGTQPA
                     DLMLHVPVDEGLARANDKVRIFGTYHRESLAVARNPQRVPALAGSAAVALEVFTREKI
                     GVETASLADSFLLSVLNGRLRENVVHFTTVAEAGRALAAGQIAAVLAPRAELEAALAG
                     QQQVVLESANFAELKIDGWPLGMAVKAEEAALAEAVGAAVADLRRDGSIAAIFRQHGI
                     TYQAN"
     gene            complement(1584..2009)
                     /gene="pedF"
                     /locus_tag="PLN02_11465"
     CDS             complement(1584..2009)
                     /gene="pedF"
                     /locus_tag="PLN02_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HOI52989.1"
                     /translation="MKHSNHSLGALFAILCLATSSLVFAHGDVVPQAVDTTGLKQLGT
                     DWLAANPYRDNKTAIRIGDSAFNQNCARCHGLGAVSGGIAPDLRYLPKGDEGDEIFLQ
                     RIRKGAVRDGRVYMPPFEGTLSQEAMWAIRAWLETVHEE"
BASE COUNT          298 a          643 c          770 g          339 t
ORIGIN      
        1 gacatcgatg gccatcaccg aaccatcgcg gccgttggag atgtagacgc gcttgccgtc
       61 cggatggatg gcgacgccgt tggggtactg gccggcggga atctcggcga tggcctggtg
      121 gctggcgacg tcgatggcgt agaccttgtc ggccagttcg caggcgacgt aggcgcggct
      181 gccgtcggcc aggaagccga tgccgcgcgg ccgcttgccg accggaatgc tggcaacctg
      241 gcggcgggcg gcaacgtcga tcacatcgac ctgttcggcg tcttcggcgc tgacgtagag
      301 ccagcggctg tcggggctga acacggcgtg ctcggggttc ttgccttgga ccttgatgcg
      361 cgccagctcc ttcgccgccg gggcgctgag cagcgtgacc acgttgtcgt cttcaacggc
      421 gacggcgagc agcttgccgt ccggcgagag gctgacgcct tccggcgaat cgccgacctt
      481 gacccggcgt tccagcttgc cggcggcgct gtccacgatg agcaggctgt tgctcttgcc
      541 gtcggtcagg tagagccggc cgctgccacc ggcgatgccg cgcggccgtt cgccgatcgg
      601 gaactggcgg acgacggtat cgctggcggt gtcgatgacg ctgacgctgg cgctcttctc
      661 gttcggcacg taggcgtagg gttcggcgct ggcgccggcg ctagcgaaca gggcgaggag
      721 gaaacaggat atggcgcgca tgttggcctc agttggcttg gtaagtgatg ccgtgctggc
      781 ggaagatggc ggcgatgctg ccgtcgcggc gcaggtcggc gacggccgcg ccgaccgctt
      841 cggcgagcgc cgcttcctcg gccttgacgg ccatgccgag cggccagccg tcgatcttca
      901 gctcggcgaa gttggcgctt tccagcacca cctgctgctg cccggccagc gccgcttcca
      961 gctcggcgcg cggggcgagg accgcggcga tctggccggc ggccagcgcc cggccggctt
     1021 cggcgacggt ggtgaagtgc acgacgttct cgcgcaggcg gccgttgagc acggaaagca
     1081 ggaaggaatc ggcgagcgag gcggtttcga cgccgatctt ctcgcgcgtg aacacttcca
     1141 gcgccaccgc ggcggacccg gcaagcgccg gcacgcgctg cggattgcgg gcgacggcca
     1201 ggctttcgcg gtgataggtg ccgaagatgc ggaccttgtc gttggcccgc gccaagcctt
     1261 cgtcgaccgg cacgtggagc atcaggtcgg cgggctgggt gccgaggtaa tggcccttcc
     1321 agaccatgtt gcgcaggtcg tcgttcatgt cctcgtcggc gttgaagccg acgatctcgg
     1381 cggcgacgcc gaggcgggcg gcgatggcac ggccgaggtc ggcgtcgatg cccttgccgc
     1441 cggccagcga gtacggcggg aagtcgttat agacggcgat gcgcaggcgg ccgcgctggc
     1501 gcactgtctc cagcgcatcg gcaaccgccg gcagggccag gcagaagggc agcgcggcaa
     1561 gcgccttgag gcagcggcgg cgatcattct tcatggacgg tctccagcca ggcgcggatt
     1621 gcccacatcg cttcctgcga cagcgtgcct tcgaacggcg gcatgtagac gcggccgtca
     1681 cgcacggcgc ccttgcggat gcgctgcagg aagatttcgt cgccctcgtc gcccttcggc
     1741 aggtagcgca gatcgggtgc gataccgccg gaaacggcgc ccaggccgtg gcagcgcgcg
     1801 cagttctggt tgaaggcgga gtcgccgatg cggatcgccg tcttgttgtc gcggtaggga
     1861 ttggcggcca gccagtcggt gccgagttgc ttcagaccgg tggtgtcgac cgcctgcggc
     1921 accacgtcgc cgtgggcaaa gaccagcgag gaagtggcca ggcacaggat ggcgaacagg
     1981 gcgccgaggg aatggttgga atgcttcacg gggtctcctt tggtgaatgc ggaacaacag
     2041 cttgtgccgg
//
LOCUS       DAOSDX010000556         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18812_length_2047_cov_3.440763, whole genome shotgun sequence.
ACCESSION   DAOSDX010000556 DAOSDX010000000
VERSION     DAOSDX010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18812_length_2047_cov_3.440763"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLN02_11470"
     CDS             <1..659
                     /locus_tag="PLN02_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI52990.1"
                     /translation="APFANTRNVTAGSIRLLGHDMTQLSADQMYDLRKNMGVAFQGGA
                     LFNSMTVGENVELPLREHTRLDDNTIRIMSRMKLEVVNLAGFEDLMPAELSGGMIKRA
                     ALARAIVMDPRLLFFDEPSAGLDPVVSAELDELILTLRDAMNMTIVVVTHELESAFKI
                     ADRITVLDQGRILMSDTAAAVRASDHPRIRALLDRTPRSDAVDADAYLERLTREIFRA
                     "
     gene            656..1579
                     /locus_tag="PLN02_11475"
     CDS             656..1579
                     /locus_tag="PLN02_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOI52991.1"
                     /translation="MKRDNVNYLLVGSFVLLMGIVLLYALYRITGQAANGEPFVTHFA
                     NVAGIKAGSPVTLDGYAIGNVASIEPVRRDGRNAYRVTLQLRQAVGIPADSRARITAP
                     GMLAAPLVDIRSGLSQEMLAAGAELPGEAGGSLSDSVAGLAGDLRALANDGIRPLVEQ
                     IAGRIDRVGGSLEENLPATLRELRSAMTRLNATAGRVDDMFSAQNRQHMEGMLKNGHD
                     ASLRIAQLSQEILGASRELEGLLKDSRLIVAGSGQDLQQSLRHADALLHQLEAAGRNL
                     NEFSRTIRNNPAALIQSRPPVDAATAQGEKP"
     gene            1576..>2047
                     /locus_tag="PLN02_11480"
     CDS             1576..>2047
                     /locus_tag="PLN02_11480"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOI52992.1"
                     /translation="MIRLLAILVLLAGLAACGGNVAPAPQEQFYRLQAVPLADAGAAA
                     GLAVGRFSADSIYAERPLVYGDPGDPHTARQYHYHLWLYPPAQLVRDHLAQTLGARQA
                     DFAERRIEGRVLRFDRVGRQAVVELELRVVQGREAIFDRRYAAEETASGDGLRAH"
BASE COUNT          339 a          741 c          678 g          289 t
ORIGIN      
        1 aggcgccgtt cgccaacacg cggaacgtga cggccggcag catccgcctg ctcggccacg
       61 acatgaccca gctgagcgcc gaccagatgt acgacctgcg caagaacatg ggcgtcgcct
      121 tccagggcgg cgcgctgttc aactcgatga cggtcggcga gaacgtcgaa ctgccgctgc
      181 gcgaacacac ccggctcgac gacaacacga tccgcatcat gagccggatg aagctggagg
      241 tggtcaacct cgccggcttc gaggacctga tgccggccga gctgtccggc ggcatgatca
      301 agcgtgcggc gctggcccgg gcgatcgtca tggatccccg gctgctcttc ttcgacgagc
      361 cgtcggccgg cctcgacccg gtggtttcgg cggaactcga cgaactgatc ctgaccctgc
      421 gcgacgcgat gaacatgacc atcgtcgtcg tcacccacga actggaaagc gccttcaaga
      481 tcgccgaccg catcaccgtc ctcgatcagg gacgtatcct gatgtcggat acggcggcgg
      541 cggtgcgcgc ttccgaccat ccgcgcatcc gcgcgctgct cgaccgcacg ccgcgcagcg
      601 acgcggtgga cgccgatgcc tacctggaac gactgacccg ggagattttc cgagcatgaa
      661 acgcgacaac gtcaattacc tgctggtcgg cagcttcgtg ctgctcatgg gcatcgtcct
      721 gctctacgcg ctgtaccgga tcaccggcca ggcggcgaac ggcgaacctt tcgtcaccca
      781 tttcgccaac gtcgccggga tcaaggccgg ctcgccggtg acgctcgacg gctacgccat
      841 cggcaacgtg gcgagcatcg agccggtgcg gcgcgatggc cgcaacgcct accgggtgac
      901 gctgcaattg cgacaggcgg tgggcatccc ggccgacagc cgggcgcgca tcacggcgcc
      961 gggcatgctg gcggcgccgc tggtcgacat ccgcagcggc ctgagccagg agatgctggc
     1021 ggccggcgcc gaactgccgg gcgaggccgg cggcagtctc agcgattcgg tcgccgggct
     1081 ggccggcgac ctgcgcgccc tggccaacga tggcatccgg ccgctggtcg agcagattgc
     1141 cggccgcatc gaccgcgtcg gcggcagcct cgaagaaaac ctgccggcga cgctgcgcga
     1201 gctgcgctcg gcgatgaccc gtctgaacgc cacggccggc cgtgtcgacg acatgttctc
     1261 ggcgcagaac cgccagcaca tggaaggcat gttgaagaac ggccacgacg cctcgctgcg
     1321 catcgcccaa ctgtcgcagg aaatcctcgg cgccagccgc gagctcgaag gcctgctcaa
     1381 ggattcccgg ctgatcgtcg ccggtagcgg ccaggacctg caacagtcgc tgcgccacgc
     1441 cgacgcgctg ctccaccagt tggaagcagc cggccgcaac ctcaacgaat tcagccgcac
     1501 catccgcaac aacccggcgg cgctgatcca gagcaggccg ccggtcgatg ccgccaccgc
     1561 ccagggagaa aaaccgtgat ccgtctgctg gccatcctcg tcctcctcgc cggcctcgcc
     1621 gcctgcggcg gcaacgtcgc gccggcgccg caggaacagt tctaccgcct gcaggcggtc
     1681 ccgctcgccg acgccggtgc cgccgccggc ctggccgtcg gccgtttcag cgccgacagc
     1741 atctacgccg agcggccgct ggtctacggt gatccgggtg atccgcatac cgcccgccag
     1801 taccactacc acctgtggct ctacccgccg gcgcaactgg tgcgcgatca cctggcgcag
     1861 acgctgggcg cccgtcaggc agatttcgcc gaacggcgga tcgagggccg ggtgctgcgc
     1921 ttcgaccgcg tcggccggca ggccgtggtc gagctcgagc tgcgcgtcgt ccagggacgg
     1981 gaggcgatct tcgaccggcg ctatgcggcc gaggaaacgg ccagcggcga cggcctgcgc
     2041 gcccacg
//
LOCUS       DAOSDX010000557         2046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18819_length_2046_cov_3.815671, whole genome shotgun sequence.
ACCESSION   DAOSDX010000557 DAOSDX010000000
VERSION     DAOSDX010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18819_length_2046_cov_3.815671"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLN02_11485"
     CDS             complement(<1..425)
                     /locus_tag="PLN02_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI52993.1"
                     /translation="MSEQAPRVLVVEDEAQIRHFVRLALEAEGCAVSEAAGVADGLAR
                     AGADDPALVVLDLGLPDGDGVDFIRSFRAWSERPVLILSARTEESHKIDALDAGADDY
                     LTKPFGVGELRARVRALMRRRQRQGGASPWLEFGNVRVD"
     gene            complement(422..1900)
                     /locus_tag="PLN02_11490"
     CDS             complement(422..1900)
                     /locus_tag="PLN02_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOI52994.1"
                     /translation="MSRLATDHLSPRQKLALSVLACVLVAIVAAPLREVLDLANMAML
                     FLLVVLLVAVSLGRGPAVLASVFGVLLFDIFFVPPHFSLAVANLQYLITFAVMLATAL
                     ITGHLTATLKEQAAEAERRERRTRALYETASRLAGALDVQQVREIVDGFVADELGGRC
                     HLLLGDSPAAIRTAASGFSATLAEAVLSDGLRRSDQEAGQRIYLPLRAPMRARGVLVV
                     DASKPPVEDELALLDALASLTAIAVERLHYVEVAQQSELQMVTERLRSSILSALSHDL
                     RTPLTVLVGLADSIALAPAVPAPVLENAAALRDQAARLAGLVGNLLEMARLNAGSVTL
                     RQEWQPLEEVVGSSLKLLGAALGRHRLHIDLAPDLPLLRFDAVLIERVLCNLLENAAK
                     YSPAAGEIRLAAELAGDWVEISVSDEGPGFPDLIAEQLFGMFVRGSGESAMSGTGLGL
                     AICRAIVDAHGGRIRIENLAGRGGRVVVALPRGEPPAVEEEA"
BASE COUNT          372 a          734 c          665 g          275 t
ORIGIN      
        1 aaatcgaccc gcacattgcc gaactcgagc cagggcgaag cgccgccctg gcgctgacgg
       61 cgccgcatca gggcgcggac gcgggcacgc agttcgccga cgccgaaagg cttggtcagg
      121 taatcgtcgg caccggcgtc gagggcatcg atcttgtggc tttcctcggt acgcgccgac
      181 aggatcagta ccggtcgttc cgaccaggcg cggaaggaac ggatgaaatc gacaccgtca
      241 ccgtcgggca accccagatc gaggacgacc agggcaggat cgtcggcgcc ggcgcgcgcc
      301 agcccgtcgg ccacgcccgc cgcctcgctc accgcgcagc cttccgcctc cagcgccagg
      361 cggacgaaat gacgaatctg cgcctcgtcc tcgacgacca gcacgcgcgg cgcctgctcg
      421 ctcatgcttc ctcctcgacc gctggcggtt cgccgcgcgg cagggcgacg acgacgcgac
      481 cgccccgccc cgccaggttc tcgatccgga tacggccgcc gtgggcgtcc acgatcgccc
      541 ggcagatcgc caggccgagc ccggtgccgg acatcgccga ctcgccgctg ccgcgcacga
      601 acatgccgaa caactgctcg gcgatgagat cggggaaacc cggcccttcg tcgctgaccg
      661 aaatctcgac ccagtcgcca gccagttcgg cggccagccg gatctcgcca gcggccggcg
      721 aatacttggc ggcgttctcg agcaggttgc acagcacccg ctcgatcaac acggcatcga
      781 aacgcagcag gggcagatcc ggcgccaggt cgatgtgcag ccggtgacga ccgagcgcgg
      841 cgccaagcag tttcaggctg ctgccgacca cctcctcgag cggctgccac tcctggcgca
      901 gggtcaccga tccggcgttg agacgggcca tttccagcag gttgccgacc agaccggcca
      961 ggcgggccgc ctggtcgcgc agcgcggcgg cgttttccag caccggcgcc ggcaccgccg
     1021 gcgccagggc gatcgaatcg gcgagcccga ccagcaccgt caacggcgtc cgcagatcgt
     1081 gcgacaaggc cgacaggatc gaactgcgca gtcgctcggt gaccatctgc aactcgctct
     1141 gttgcgccac ctcgacgtaa tgcaggcgct cgacagcaat tgccgtcagc gaagcaagcg
     1201 catcgagcag cgccagttcg tcctcgaccg gcggcttgct ggcgtcgacg accagcacgc
     1261 cgcgcgcccg catcggggcc cgcaagggca gatagatccg ctggccggct tcctgatcgc
     1321 tgcggcgcag accgtcgctc agcaccgctt ccgccagcgt cgcagaaaac ccgctggcag
     1381 cggtgcggat cgccgccggg ctgtcgccga gcagcaaatg acagcgcccg cccagttcat
     1441 cggcgacgaa accatcgacg atctcccgca cctgctgcac atcgagcgcc ccggccaggc
     1501 ggctggctgt ctcatagagc gcgcgcgtcc gccgttcgcg gcgttccgcc tcggccgcct
     1561 gctccttcaa ggtcgccgtc aggtggccgg tgatcagcgc cgtggccagc atcaccgcga
     1621 aggtgatcag gtattgcagg ttggcgaccg ccagcgagaa atgcggcggc acgaagaaga
     1681 tgtcgaacaa caggacgccg aacaccgagg ccagtaccgc cggtccgcgc cccagactga
     1741 cggcgacgag aagaacgacg agcaggaaga gcatcgccat gttggccagg tcgagcacct
     1801 cgcgcagtgg cgcggcgacg atggcgacca gcacgcaggc aaggacgctc agtgccagtt
     1861 tctgacgcgg cgagagatga tcggtggcga ggcgtgacat gttcagcggc actctaaatg
     1921 acgcgcaatg aagcgtgcgc gcattctggc aacagccgcg taaaaatgtc gtaaaaattc
     1981 gccggccggc gatctctgct aagctcggcc tcacaccctg cacgcttctt ccccgagcca
     2041 gcgcgg
//
LOCUS       DAOSDX010000558         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18843_length_2044_cov_3.785319, whole genome shotgun sequence.
ACCESSION   DAOSDX010000558 DAOSDX010000000
VERSION     DAOSDX010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18843_length_2044_cov_3.785319"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2044
                     /locus_tag="PLN02_11495"
     CDS             <1..>2044
                     /locus_tag="PLN02_11495"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOI52995.1"
                     /translation="LIQRDPAKIDQNEFLVVNQFTVVEDRRNRRPDVVVFVNGLPLGI
                     IELKNPAEEKATIRHAFNQLQTYKLDIPGFFVFNEMMVNSDGLFAKAGTLTAGWDRFQ
                     PWRTTDGSTLAPKSAVQLEVLIKGVFEKRRFLDYVLNFITFEDDGKAIAKKAAAYHQF
                     WAVNKAVDCTVEAISPCGDRRIGVVWHTQGSGKSLSMAFYGGKIIKHPAMENPTLVVI
                     TDRNDLDDQLFGTFSTNSELLRQKPVQAESREHLKELLRVASGGVVFTTLQKFEGGDA
                     LSDRRNIVVIADEAHRSHYGFRARLDKKTGELKYGMAKYLRDSLPGASFIGFTGTPVE
                     LDDKNTPAVFGDYIDKYDILRAVEDGATVPIYYESRLAKLELKDEEKPKLDPEFEEIT
                     EGEEEEEKSKLKTKWAALEAMVGTDKRVRLVAQDIIDHFEKRVGAMVGKAMIVCMSRR
                     ICVDMYDSIIKLRPQWHHADDAKGQIKIVMSGSASDNTTWQPHIRTKIASEAIAKRYK
                     DPSDGLKLVIVRDMWLTGFDCPSMHTMYLDKPMNGHNLMQAIARVNRVFKDKPGGLVV
                     DYLGIADALKHALHTYTASGGKGQATVDQEQAAAVMVEKFEVVCGLMHGFDYKAILKA
                     APAQRMNGVAQAMEFVLSLDKGKARFVQAVSNLSKAFALAVPNAKAMAIRDEIGLFQ"
BASE COUNT          489 a          533 c          623 g          399 t
ORIGIN      
        1 ttgatccaac gcgacccggc taagattgac cagaacgagt ttctcgtcgt caaccagttc
       61 acggtcgtcg aggatcggag aaaccgcagg ccggacgtag tggtttttgt caacggcctg
      121 ccgctcggca tcatcgagct gaaaaatccc gccgaggaaa aagcgacgat ccgccatgcc
      181 ttcaaccagc tccaaacgta caaattggac atccccggtt tcttcgtttt caacgagatg
      241 atggtgaact ccgacggtct tttcgcgaaa gcgggaacgt tgaccgccgg ttgggatcgg
      301 tttcaaccgt ggcggaccac tgatggaagc accctcgcac ccaagagcgc ggtgcaactg
      361 gaagtgttga tcaaaggggt cttcgagaaa cgacggtttc ttgattatgt gctaaatttc
      421 atcacgtttg aggatgacgg caaggcgatt gccaagaaag cggcggccta tcaccagttt
      481 tgggcggtca acaaggcggt ggattgcacg gtcgaggcca tttccccctg cggcgatagg
      541 cgcattggcg tggtatggca cacgcaaggc tctggcaaaa gcctttcaat ggccttctac
      601 ggcgggaaga tcatcaagca tccggcgatg gagaacccga cgctggtcgt cattacggat
      661 cgcaatgacc tcgacgatca gcttttcggc accttcagca ccaatagcga gcttctgcgc
      721 cagaaacctg tccaggccga gagccgggag cacctgaaag agctgctgcg ggtggcatcg
      781 ggcggcgtgg tgttcaccac gttgcaaaag tttgagggcg gtgacgcctt gagcgaccga
      841 cgcaatatcg tggtgatcgc cgacgaagct caccgcagcc attacggatt tcgtgccaga
      901 ctggacaaaa agaccggcga attgaagtac ggcatggcca agtatctgcg ggattctttg
      961 ccgggcgctt cgttcatcgg attcaccggt acgccggttg agctcgatga caagaacacg
     1021 ccagcagtct tcggcgatta tatcgataag tacgacatcc tccgagcggt cgaagatggc
     1081 gcgacggtcc caatttacta cgagtcgcgc ttggccaagc tggaactgaa ggatgaggag
     1141 aagccgaaac tcgatccgga gtttgaggaa atcaccgaag gcgaagaaga agaggaaaag
     1201 agcaagctca agaccaaatg ggcggcgctg gaggcaatgg tcggcaccga caagcgggtg
     1261 cggctggttg cgcaggacat catcgatcac tttgaaaagc gcgtcggggc gatggtcggc
     1321 aaggccatga tcgtctgcat gtcgcggcgc atctgtgtgg acatgtacga ttctatcatt
     1381 aaactgcgcc cgcaatggca ccatgcggat gatgccaagg gccagattaa aatcgtcatg
     1441 agcggttcgg catcggacaa tacgacctgg cagccgcaca ttcgcaccaa aatagccagc
     1501 gaagccatcg ccaagcggta caaggaccca tccgacgggc ttaaactggt tatcgtgcgg
     1561 gacatgtggc tgacgggatt cgactgcccc agcatgcaca cgatgtacct ggacaagccg
     1621 atgaacgggc acaacctgat gcaggccatc gcacgggtca atcgcgtgtt caaggataag
     1681 cccggcggcc ttgtcgtgga ctatcttgga atcgcggacg cgctaaagca cgctttgcat
     1741 acctatacgg ccagtggcgg taagggtcag gcgaccgttg atcaggagca ggcggcggca
     1801 gtcatggtcg agaagttcga ggtagtgtgc ggccttatgc atggctttga ctacaaggcc
     1861 atcctgaagg cggctcccgc tcaacgcatg aacggcgtag cccaggccat ggaattcgtg
     1921 cttagtttgg ataagggcaa agcgcgcttt gttcaggcgg taagcaatct ctcgaaagcg
     1981 tttgcgctgg cggtgccgaa tgcgaaagcg atggcgatac gtgatgaaat cggactattc
     2041 cagg
//
LOCUS       DAOSDX010000559         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18880_length_2040_cov_3.022670, whole genome shotgun sequence.
ACCESSION   DAOSDX010000559 DAOSDX010000000
VERSION     DAOSDX010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18880_length_2040_cov_3.022670"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLN02_11500"
     CDS             complement(<1..896)
                     /locus_tag="PLN02_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI52996.1"
                     /translation="MSAISDRMSPAERRVGASLASIFALRMLGLFLILPVFSAHAASI
                     PGGGNLLLVGLALGAYGLTQALFQIPFGIASDLVGRKPVIVTGLLLFAVGSFVAAWAP
                     DLHWIIAGRVLQGMGAISAAVTALAADLTREEHRTKVMAMIGSSIGLVFALSLVGAPL
                     LYGWVGLSGLFVLTGVLALAAIGLLLKTVPPVPRPAGHVKLPLRKVLADPALLKLNFG
                     IGTLHMIQMAMFVVLPQALLHRGGLAAAEHWKVYLPAVVASFAFMVPAIIAAERRDQM
                     RPVFLAAVGLTALVQGGLFLFS"
     gene            1045..1782
                     /locus_tag="PLN02_11505"
     CDS             1045..1782
                     /locus_tag="PLN02_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOI52997.1"
                     /translation="MSKKNSIGRQGIAAGLVIATLGSAGCGSATRVFDPPPPPAMEQD
                     AQPPAPVAASVAAPAVAPAVAPAVAPAVAPAVAPAVAPAVAPAVAPAAATPAAPAVAA
                     TVAPPAVASGAPASATPATAAPPPAAAPADRSLQDFVDGWRDAWVARDVNAYFKHYSP
                     EFKGQASSAPEWRSARQRVIGRAGQIELHLGKLEIRREGDDRAWLTFEQRYKTQSYTD
                     SGIKQLQLRRVDGKWLIEQEVFSTAKP"
     gene            1880..>2040
                     /locus_tag="PLN02_11510"
     CDS             1880..>2040
                     /locus_tag="PLN02_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI52998.1"
                     /translation="METLRIRGARTHNLKNINLDLPRDQLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYV"
BASE COUNT          370 a          714 c          675 g          281 t
ORIGIN      
        1 tcgctgaaca ggaacaggcc gccctggacc agcgcggtga gcccgaccgc cgccaggaac
       61 accggccgca tctggtcgcg gcgttcggcg gcgatgatcg ccggcaccat gaaggcgaag
      121 gaggcgacga ccgccggcag atagaccttc cagtgctcgg cggcggccag cccgccgcgg
      181 tgtagcagcg cctgcggcag gacgacgaac atcgccatct ggatcatgtg cagggtgccg
      241 atgccgaaat tgagcttgag cagcgccggg tcggccagca ccttgcgcag cggcagcttg
      301 acgtgaccgg ccgggcgcgg caccggcggc accgtcttca gcagcaggcc gatcgccgcc
      361 agcgccagca cgccggtcag cacgaacagg ccgctcaggc cgacccagcc gtagagcagc
      421 ggcgcgccga ccagcgacag cgcgaagacc aggccgatcg acgaaccgat catcgccatc
      481 accttggtcc ggtgctcctc gcgcgtcagg tcggcggcga gcgcggtgac cgctgccgag
      541 atcgccccca tgccctgcag cacgcggccg gcgatgatcc agtggagatc cggcgcccag
      601 gcggcgacga aactgccgac ggcgaacagc aacaagccgg tgacgatcac cggcttgcgt
      661 ccgacgaggt cggaagcgat gccgaaggga atctggaaga gcgcctgcgt caggccatag
      721 gcgccgagcg ccaggccgac cagcagcagg ttgccgccgc cgggaatgct cgccgcgtgc
      781 gcggagaaca ccggcaggat caggaacagg ccgagcatgc gcagggcgaa gatcgaagcc
      841 agcgaagcgc cgacgcgacg ttcggcgggg ctcatgcggt cggaaatggc ggacatggga
      901 atttgttgca atgcagcgag gaaaacgagt ttagcaggtt tgccccggcg cagccgctgc
      961 ccctacgcaa ggcctaggcc ataggttata gtcgcgcctt gttgttgtca ttattggcca
     1021 acgctttcac gatcgggagg gatcatgagc aagaagaaca gcatcggcag gcaggggatt
     1081 gccgccggac tcgtcattgc aacgctgggc tccgctggct gcggcagcgc gacgcgggtg
     1141 ttcgatccac cgccgccgcc ggcgatggag caggacgccc aaccgccggc gccggtcgcc
     1201 gcgtcggtcg ccgcgccggc tgttgcaccg gctgttgcac cggctgttgc accggctgtt
     1261 gcaccggctg ttgcacccgc tgttgcaccc gctgttgcac ccgctgttgc acccgcagcg
     1321 gcaaccccgg cagcgcccgc cgtcgccgcg accgtcgccc cgccggccgt cgccagcggc
     1381 gccccggcca gtgccacgcc cgccacggca gcgccgcccc cggctgccgc ccccgccgac
     1441 cgcagcctgc aggacttcgt cgatggctgg cgcgatgcct gggtcgcccg cgacgtcaac
     1501 gcctacttca agcactactc cccggaattc aaggggcagg caagcagtgc cccagagtgg
     1561 cgcagcgccc gccaacgcgt gatcggccgc gccgggcaga tcgaattgca tctgggcaag
     1621 ctggaaatcc gccgcgaagg cgacgaccgc gcctggctga ctttcgagca gcgctacaag
     1681 acgcagtcgt acaccgattc cggcatcaag cagttgcaat tgcgccgggt cgacggcaag
     1741 tggctgatcg agcaggaagt gttctcgacc gccaagcctt gaccccagcg gaaagctgta
     1801 cattcataca gctttccgtg caaggcgcgc cgcttttgcg tatagttccc gcttcgtccc
     1861 aaccccaggt agaccggcga tggaaactct ccgcatccgc ggcgcccgca cccataatct
     1921 caagaacatc aacctcgacc tgccgcgcga tcagctgatc gtgatcaccg gcctgtccgg
     1981 ctcaggcaag tcctcgctgg ctttcgacac gctctacgcg gaagggcagc gccgctacgt
//
LOCUS       DAOSDX010000560         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18922_length_2036_cov_3.915699, whole genome shotgun sequence.
ACCESSION   DAOSDX010000560 DAOSDX010000000
VERSION     DAOSDX010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18922_length_2036_cov_3.915699"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1103
                     /locus_tag="PLN02_11515"
     CDS             105..1103
                     /locus_tag="PLN02_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOI52999.1"
                     /translation="MIPKLKTTLLALSCLLAGAVHAQSSLLNVSYDVSRELYKEINPA
                     FVAAWKKQGGADVTINQSHGGSSKQAGAVIGGLEADVITMNQAPDIDILVERGGLVAA
                     DWRKQFPHEATPYTTTTVFLVRKGNPKQIKDWGDLTRAGLQVIVPNPKTSGNGRYTYL
                     AAWGYALDKSGSDAGAKDFVSKLFANVPVLDGGGRGATTTFTQRDVGDVLVTFENEAI
                     LIAQELGSSKFDIVYPSLSIEAAAPVAVVQKVAAKRGSEKLARAYLDFLFSPAGQEII
                     AKHDFRPRDPAVLKKLAAKYPAIRTFTVEQKLGGWAAVQKAHFADGALYDQIVARK"
     gene            1103..1939
                     /gene="cysT"
                     /locus_tag="PLN02_11520"
     CDS             1103..1939
                     /gene="cysT"
                     /locus_tag="PLN02_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOI53000.1"
                     /translation="MASAKTFRVLPGFNLTLGYTLGYLSLLILIPLAAVFLKAFELDW
                     ERFVEVVTAPQVVATYKLSFGASLLAAAINAVFGLLLAWSLVRYSFPGKKLVDALVDL
                     PFALPTAVAGIALTALYAKNGWLGQYLEPLGIKVAFGPLGVLVALIFIGLPFVVRTVQ
                     PILEDLDTELEEAAASLGAQRWQTFRHVILPILLPALLTGFALAFARAVGEYGSVIFI
                     AGNLPYVSEITPLMIITKLEQYDYRGATAIAAVMLLFSFTLLLIINGLQAWTAKRTGR
                     DR"
BASE COUNT          339 a          703 c          628 g          366 t
ORIGIN      
        1 ccggctttat ccgttttgtg gataacgaaa tcagtttttc ttcgttgggc ggcgctgtcg
       61 ctctccctag actgcgctca tcaatcacaa gaacggagcc cccgatgatc cccaagctca
      121 agaccaccct gctcgcgctg tcctgcctgc tggccggtgc ggtgcatgcc cagagctcgc
      181 tgctcaacgt ttcctacgac gtctcgcgcg agctgtacaa ggaaatcaac ccggccttcg
      241 tcgccgcctg gaagaagcag ggcggggcgg acgtcaccat caaccagtcg cacggcggtt
      301 cgtcgaagca ggccggggcg gtgatcggcg gcctggaagc cgacgtcatc acgatgaacc
      361 aggcgccgga catcgacatt ctggtcgagc gcggcggcct ggtcgccgcc gactggcgca
      421 agcagttccc gcacgaggcg acgccgtaca cgacgaccac cgtcttcctg gtgcgcaagg
      481 gcaatccgaa gcagatcaag gactggggcg acctgacccg cgccggcctg caggtcatcg
      541 tgcccaaccc gaagacttcc ggcaacggcc gctacaccta cctcgccgcc tggggttacg
      601 ccctcgacaa gagcggttcc gacgccggcg ccaaggactt cgtcagcaag ctgttcgcca
      661 acgtgccggt gctcgacggc ggtggccgcg gcgcgaccac caccttcacc cagcgcgatg
      721 tcggcgatgt gctggtgacc ttcgagaacg aagccatcct gatcgcccag gaactcggtt
      781 cgagcaagtt cgacatcgtc tatccgtcgc tgtcgatcga ggccgcggcg ccggtcgccg
      841 tcgtccagaa ggtggccgcc aaacgcggca gcgagaagct ggccagggcc tacctcgact
      901 tcctgttttc gccggccggc caggaaatca tcgccaaaca cgacttccgg ccgcgcgacc
      961 cggcggtatt gaagaaactt gccgccaagt acccggcgat ccgcaccttt accgtcgaac
     1021 agaagctcgg tggctgggcc gccgtgcaga aggcgcattt cgccgacggc gcgctctacg
     1081 accagatcgt cgcgaggaag tgatggccag cgccaaaacc ttccgcgtcc tgccggggtt
     1141 caacctgacc ctcggctaca cgctcggcta cctgtcgctg cttatcctga tcccgctcgc
     1201 cgcggtcttc ctgaaggcct tcgagctgga ctgggaacgc ttcgtcgagg tggtgacggc
     1261 gccgcaggtg gtcgccacct acaaactctc gttcggcgcc tcgttgctgg cggcggcgat
     1321 caacgcggtg ttcgggctgc tgctcgcctg gtcgctggtg cgttactcgt ttcccggcaa
     1381 gaagctggtc gatgccctgg tcgacctgcc gttcgcgctg cccaccgccg tcgccggcat
     1441 cgcgctgacc gcgctgtacg cgaagaacgg ctggcttggc cagtacctcg aaccgctcgg
     1501 catcaaggtc gcgttcggac cgctcggcgt gctggtggcg ctgatcttca ttggcctgcc
     1561 tttcgtcgtg cgtaccgtgc agccgatcct cgaagacctc gacaccgaac tcgaggaggc
     1621 cgccgccagc ctcggcgcgc agcgctggca gaccttccgc cacgtcatcc tgccgatcct
     1681 gctgccggcg ctgctgaccg gcttcgcgct ggcctttgcg cgcgccgtcg gtgagtacgg
     1741 ctcggtcatc ttcatcgccg gcaacctgcc ttatgtttcc gagatcacgc cgctgatgat
     1801 catcaccaag ctcgaacagt acgactaccg cggcgccacg gcgattgccg cggtgatgct
     1861 gctgttttcc ttcacgctgc tgctgatcat caacgggctg caggcgtgga ccgccaaacg
     1921 taccgggagg gaccgctgat ggctggcgca accacgatgc atctcggcgc cgaccatgcg
     1981 tcgcgcttcg agagcaaggc ggcgaccagc gagacgccgc tggtcaaatg gacgat
//
LOCUS       DAOSDX010000561         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18958_length_2032_cov_3.519980, whole genome shotgun sequence.
ACCESSION   DAOSDX010000561 DAOSDX010000000
VERSION     DAOSDX010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18958_length_2032_cov_3.519980"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLN02_11525"
     CDS             <1..693
                     /locus_tag="PLN02_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOI53001.1"
                     /translation="RVLEHVVDTVLYFEGDTHSSFRLVRAVKNRFGAVNELGVFAMTE
                     SGLKGVSNPSAMFLSQHGQEVAGSCVMVTQEGTRPLLVEIQALVDTAHGNPRRLTVGL
                     DAQRLAMLLAVLHRHAGIVCFDQDVFVNAVGGVKIGEPAGDLAVIMAITSSLKNKALP
                     EKLIVFGEVGLAGEIRPAPRGQERLKEAAKLGFTRALIPEANRPRQAIPGMEVIAVRR
                     VEEAVQRIRELQ"
     gene            690..>2032
                     /locus_tag="PLN02_11530"
     CDS             690..>2032
                     /locus_tag="PLN02_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOI53002.1"
                     /translation="MNLTLAWRMLRRELRAGELHLLFAALTIAVAAVSAVGFLADRVG
                     RGLEGEARQMLGADLVVAADQPLPAELAAEAGRRGLRTTQTLVFPSMVFANGRAELVE
                     IKAVGAGYPLRGRLRTAAAPGQPDLAVDGGPQPTTAWADERLFSLLALEPGAPLGVGQ
                     LQLPVSAVLTREPDRSFNVFALQPRLLMHLDDIAGSGLIQFGARVRYRLLVAGEAAAL
                     AQWRAWAEPRLGRGQRLEGIENARPEIRNALDKAQRFLGLATLLTVILSAIAVALAAR
                     RYMQRHLDACAVLRCLGMTQGRLLRLHALSFFWLAALAAVLGAGLGFAAHFALVSAMA
                     GLIAGELPLPGAGPLGEGALVAGVLLFGFALPPLLQLARVPTLRVLRRELGPPAPLAV
                     LGYAFGFALLAMLIVRAAGDLRLGLFAVAGFSGALALFWGLAWSAVRIAGRLRGGA"
BASE COUNT          248 a          728 c          726 g          330 t
ORIGIN      
        1 cgcgtgctcg aacacgtggt cgataccgtg ctctatttcg agggcgacac gcattcgagc
       61 ttccgcctgg tgcgcgcggt caagaaccgc ttcggcgcgg tcaatgagct cggcgtcttc
      121 gcgatgaccg agagcggcct caagggcgtc agcaatccgt cggcgatgtt cctgtcgcag
      181 cacggccagg aggtcgccgg ctcctgcgtg atggtgacgc aggaggggac gcggccgctg
      241 ctggtggaaa tccaggcgct ggtcgacacc gcgcacggca acccgcgccg gctgaccgtc
      301 ggcctcgatg cgcagcgact ggcgatgttg ctggcggtgc tgcaccgcca cgccggcatc
      361 gtctgcttcg accaggacgt cttcgtcaac gccgtcggcg gggtcaagat cggcgagcca
      421 gcaggcgatc tggcggtgat catggcgatc acgtcttcgt tgaaaaacaa agctttgcca
      481 gagaaactta tagtatttgg tgaagttggt ctggcgggtg aaatccgccc cgcgccgcgc
      541 ggccaggagc gtttgaagga ggccgccaag ctcggcttca cccgcgccct gatccccgag
      601 gccaaccggc cgcggcaggc gatcccgggc atggaagtga tcgccgtgcg ccgcgtcgag
      661 gaagcggtgc agcgcatccg ggagctgcag tgaacctgac gctggcctgg cgcatgctgc
      721 gccgcgaact gcgcgccgga gagctgcacc tgcttttcgc ggcgctgacc atcgccgtcg
      781 ccgcagtcag cgccgtcggt tttctcgccg accgggtcgg ccgggggctt gagggtgagg
      841 cacggcagat gctcggcgcc gacctggtgg tcgctgccga ccagccgctg ccggccgaac
      901 tcgctgcaga ggccgggcgc cgcggcctgc gcacgacgca gacgctggtc ttcccaagca
      961 tggttttcgc caacggccgg gcggaactgg tcgagatcaa ggcggtcggc gccggctacc
     1021 cgctgcgcgg ccgcctgcgc accgccgccg cgccgggcca gccggatctc gcggtcgacg
     1081 gcggcccgca gccaacaacc gcctgggccg acgagcgcct gttcagcctg ctcgcacttg
     1141 aacccggcgc gccgctcggc gtcggccagc tgcagctgcc ggtgagcgcc gtgctcaccc
     1201 gcgaacccga ccgcagcttc aacgtcttcg cgctgcagcc gcgcctgctg atgcacctcg
     1261 acgacatcgc cggcagcggc ctgatccagt tcggcgcgcg cgtccgctac cgcctgctgg
     1321 tcgccggcga ggcggcggcg ctggcgcaat ggcgggcctg ggccgagccg cggctggggc
     1381 gcgggcagcg cctcgaaggc atcgaaaacg cccgcccgga aatccgcaac gcgctcgaca
     1441 aggcgcagcg ctttctgggc ctggcgacgc tgttgaccgt gatcctctcg gccatcgccg
     1501 tggcgctcgc cgcccgccgc tacatgcagc gccacctcga cgcttgcgcc gtgctgcgct
     1561 gcctgggcat gacccagggt cggctgctgc gcctgcatgc gctgtccttt ttctggctgg
     1621 cggcgctggc cgcggtgctt ggcgccggcc tcggctttgc cgcgcatttc gcgctggtct
     1681 cggcaatggc cgggctgatc gccggcgagt tgccgctgcc cggcgccggc ccgctcggcg
     1741 aaggggcgct ggtcgccggc gtgctgctct tcggcttcgc gctgccgccg ctgctgcaac
     1801 tggcgcgggt gccgaccttg cgcgtgctgc gccgcgaact cggaccgccg gcgccgctgg
     1861 ccgtgctcgg ctacgctttc ggctttgcac tgcttgccat gctgatcgtc cgcgccgccg
     1921 gcgacctgcg cctcggcctg ttcgccgtcg ccggcttcag cggcgcgctg gcgctgttct
     1981 gggggctcgc ctggagcgct gtccgcatcg ccggccggct gcgcggcggc gc
//
LOCUS       DAOSDX010000562         2032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18962_length_2032_cov_3.313606, whole genome shotgun sequence.
ACCESSION   DAOSDX010000562 DAOSDX010000000
VERSION     DAOSDX010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18962_length_2032_cov_3.313606"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..553
                     /locus_tag="PLN02_11535"
     CDS             155..553
                     /locus_tag="PLN02_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53003.1"
                     /translation="MRLELCLLAALLGAPALSAAAADGPWLRCRLEQSNTVHVLGYGP
                     AADIYRVPATNINDNFHFKAVVAAADGVAEYVKIYVYYQRRGQPILLQQATYRAPIAD
                     GRSLTGEQRLYSPILGRELQYDCALTSVRP"
     gene            550..1518
                     /locus_tag="PLN02_11540"
     CDS             550..1518
                     /locus_tag="PLN02_11540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOI53004.1"
                     /translation="MRSLLLLAACLLPLPVWADAGTVAVAFVGDVMLDDTPGKVIRQG
                     RDPLVPFAAILDSADLRIANLECVVARGGKAEADKPYTFRAPPRVLQLVKRHFDAVGL
                     ANNHSGDFGPAAFAEMLGHLDKAGLGYFGGGRTLADAHRPLIVERKGLRIALLGYNEF
                     FPRSFEAGHNQPGIAWSEDEQVLHDIRQARERDRADLVIPVMHWGWEHETRASERQKA
                     LARRMIDAGADAVVGGHPHVTQEIEIYRGKPVIYSLGNFVFDGFDDPLNNTGWLLRLE
                     LDKAGVVSSRIVVAKIDREGTPHPAPQAASACWERGQAAPADCRGR"
     gene            1637..>2032
                     /locus_tag="PLN02_11545"
     CDS             1637..>2032
                     /locus_tag="PLN02_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOI53005.1"
                     /translation="MLEAVGNSLAGMAWPLALALAWLAGEAVSRWLSLPRVCGYALAG
                     FVLASGQLGLLPAPAGTPVARLADFAIALVLFELGQHINLNWLRHNRWLAVTSLVEAF
                     ATFIAVLLVARVFGLAPLPALLLAALAMSS"
BASE COUNT          302 a          710 c          671 g          349 t
ORIGIN      
        1 gcaatgcgcc gacggcgttg tgcatctggt tgcccagcgc ctggtcgacc tgtcggcctg
       61 gctggggcgg ctggaaacgc gcagtcggga ttttcactaa gcggatgcgg cggttttccc
      121 ggttatgatg ctggccacag gggagggaaa cgcgatgcgt cttgagcttt gtctgctggc
      181 cgcgctgctc ggggcgccgg cactatccgc cgccgccgcc gacgggccgt ggctgcgctg
      241 ccggctggaa cagtcgaaca ccgtccacgt cctcggctac ggaccggccg ccgacatcta
      301 ccgggtgccg gcgaccaaca tcaacgacaa cttccacttc aaggccgtcg tcgccgctgc
      361 cgacggcgtc gccgagtacg tcaagatcta cgtctactac cagcgccgcg gccagcccat
      421 cctgttgcag caggcgacct accgcgcgcc gattgccgac ggccgttcgc tgaccggcga
      481 acagcgcctc tattcgccca tcctcgggcg cgaactgcag tacgactgcg ccctgacgag
      541 tgtccgccca tgagatcgct gctcctgctt gccgcctgcc tgctgccgct tccggtgtgg
      601 gccgatgccg gtaccgttgc ggtcgccttc gtcggtgacg tgatgctcga cgacacgccg
      661 ggcaaggtga tccgccaggg ccgcgacccg ctggtgccgt tcgccgccat tctcgacagc
      721 gccgacctgc gcatcgccaa cctcgagtgc gtcgtcgccc gcggcggcaa ggcggaagcc
      781 gacaagccct ataccttccg cgccccgccg cgcgtgctgc agctggtgaa acgccatttc
      841 gacgccgtcg gcctggccaa caaccattcc ggcgatttcg gcccggccgc ctttgccgaa
      901 atgctcggcc acctcgacaa ggccggtctc ggttatttcg gcggcgggcg cacgctggcc
      961 gacgcccatc ggccgctgat cgtcgagcgc aaggggctgc gcatcgcgct cctcggctac
     1021 aacgagttct tcccgcgcag cttcgaggcc ggccacaacc agccgggcat cgcctggagc
     1081 gaggacgagc aggtgctgca cgacatccgc caggcgcgcg agcgcgaccg ggccgacctg
     1141 gtgattccgg tgatgcactg gggctgggag cacgaaacac gggccagcga gcggcagaaa
     1201 gcgctggcgc ggcggatgat cgacgccggt gccgacgccg tcgtcggcgg ccatccgcac
     1261 gtcacccagg aaatcgagat ctaccgcggc aagccggtga tctacagtct cggcaacttc
     1321 gtcttcgacg gcttcgacga cccgctcaac aacaccggct ggctgctgcg tctggaactc
     1381 gacaaggccg gcgtcgtcag ttcgcgcatc gtcgtcgcca aaattgaccg cgaaggcacg
     1441 ccgcacccgg caccgcaggc cgccagcgcc tgctgggaac gtggtcaggc ggcaccggcg
     1501 gattgccggg gacgctgatg gcacgatagc gggaactctc ggcgattcgt tcggccagat
     1561 actgacatgg gggtcaaacc tgcagtaatc tgaagcacgc gcaagacaca agcgactcac
     1621 cacgggagtg cgcatgatgc ttgaggctgt tggaaattcc ctggccggca tggcctggcc
     1681 gctggccctg gcgctggcct ggctggctgg cgaagcagtc agccgctggc tgtccttgcc
     1741 gcgcgtctgc ggttacgcgc tggccggctt cgtgttggcc agcggtcagc tcggcctgtt
     1801 gccggcgccc gccggaacgc cggtggcgcg gctggcggat ttcgccattg cgctggtgct
     1861 gttcgaactt ggccagcaca tcaacctgaa ctggttgcgc cataaccgct ggctggccgt
     1921 gaccagcctg gtcgaggcct tcgctacctt tatcgccgtg ctgctggtcg cccgcgtctt
     1981 cggcctggcg ccgctgcctg cccttctgct ggccgcgctg gcgatgtcct cg
//
LOCUS       DAOSDX010000563         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18974_length_2031_cov_3.196862, whole genome shotgun sequence.
ACCESSION   DAOSDX010000563 DAOSDX010000000
VERSION     DAOSDX010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18974_length_2031_cov_3.196862"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /locus_tag="PLN02_11550"
     CDS             complement(<1..1729)
                     /locus_tag="PLN02_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOI53006.1"
                     /translation="MHANPPLAGPLLRQARLKLLDGGNFSDSEIDERLARSWRRSLAA
                     GLTPTGRLAVPDNLTAGELGRALGRNRELLNHSRPIMEYLFEQVRHSHSLVVLADNHG
                     VLMHTLGDTDFLGKAERVALMSGACWDERQRGTNAIGTALAEAAGIEIHGDEHFLERN
                     TFLTCAAAPIVSATGELLGILDISGDRRSRHPHTLGLVNTAARMIENRLVADACRRHL
                     RLYLHPDPAGIGTVAEGIVALSEDGWIIGANRNALRLLGLSAADIGGTPLFRAIDTRI
                     GDLIAQDRRRPGQVLALHGHDGRTFYARIDGHEPRVLSVPKTSSPQHNDALTALDTGD
                     LRWRAAADKARRILGKDIPLLLSGESGVGKELFARAVHESSPQRNGPFVAINCAALPE
                     NLIEAELFGYQPGAFTGARKEGYIGRLREAEGGTLFLDEIGDMPLALQTRLLRVLQER
                     KVLPLGGGVAVDVRFSLICATHRDLPAASAAGQFRSDLYYRINGLTLNLPALRERNDF
                     AALCDKLLAEFFPDAPLAIEPALQHRLAGYAWPGNLRQLASVLRTACAMLDPGESLID
                     WPHLPDDLVD"
BASE COUNT          322 a          609 c          760 g          340 t
ORIGIN      
        1 cgtcgaccag atcgtccggc aggtgcggcc agtcgatcag cgattcgcca gggtcgagca
       61 tggcgcaggc agtgcgtagc acgctggcca gttgccgcag gttgcccggc caggcatagc
      121 cggcgagacg gtgctgcaat gccggttcga tggccagcgg ggcgtccggg aagaactcgg
      181 cgagcagctt gtcgcacagc gcggcgaagt cgttgcgttc gcgcagcgcc ggcaggttca
      241 gcgtcaggcc gttgatccgg taatagaggt cgctgcggaa ctggccggcg gcgctggctg
      301 ccggcagatc gcggtgggtg gcgcagatca gcgagaagcg gacgtcgacc gcgacgccac
      361 caccgagcgg cagcaccttg cgttcctgga ggacgcgcag caggcgggtc tgcagcgcca
      421 gcggcatgtc gccgatttcg tcgaggaaca gggtgccgcc ttcggcttcg cgcaggcggc
      481 cgatgtagcc ctccttgcgg gcgccggtga aggcgccggg ctggtagccg aagagttcgg
      541 cttcgatcag gttttccggc agcgcggcgc agttgatggc gacgaatggg ccgttgcgct
      601 gcggcgagga ttcatgcacg gcacgggcga acaattcctt gccgacgccg gattcgccgc
      661 tgagcagcag cgggatgtcc ttgccgagga tgcgccgcgc cttgtcggcc gccgcccgcc
      721 agcgcaggtc gccggtgtcg agcgcagtca gcgcgtcgtt atgttgcggg ctgctggttt
      781 tcggcaccga cagcacgcgt ggctcgtgac cgtcgatccg cgcatagaag gtcctgccgt
      841 cgtggccgtg cagggcgagc acctgcccgg ggcggcgccg gtcctgggca atgaggtcgc
      901 caatccgggt atcgatggcg cgaaacagcg gggtgccgcc gatatcggcg gcagacagac
      961 caagcaggcg cagcgcgttg cggttggcgc cgatgatcca gccgtcctcg gaaagggcga
     1021 cgatgccttc ggcgacggtg ccgatgccgg ccgggtccgg atgcaggtag aggcgcaggt
     1081 ggcggcggca ggcgtcggcg accagccggt tctcgatcat ccgggcggca gtgttgacca
     1141 ggcccagggt gtgcggatgg cggctgcggc ggtcgccgga aatgtcgagg atgccgagca
     1201 gttcgccggt ggcggagacg atcggcgcgg cggcgcaagt gaggaaggtg ttgcgttcga
     1261 ggaaatgttc gtcgccgtgg atctcgatgc cggccgcctc ggccagcgcg gtgccgatgg
     1321 cgttggtgcc gcgctgccgc tcgtcccagc aggcgccgga catcagcgcg acgcgctcgg
     1381 ccttgccgag gaaatcggtg tcgcccagcg tgtgcatgag gacgccgtga ttgtcggcga
     1441 ggacgaccag gctgtgactg tggcgcacct gctcgaacag gtattccatg attggccgcg
     1501 agtggttgag cagttcccgg ttgcgcccca gggcgcggcc cagttcgccg gccgtcagat
     1561 tatcggggac ggccaggcgg ccggttggcg tcaggccggc ggcgagactg cgccgccagg
     1621 agcgggccag gcgttcgtcg atttcgctgt cggaaaagtt gccgccgtcg agcagcttga
     1681 gcctggcctg gcgcaagagc gggccggcaa gcgggggatt tgcgtgcatc gggattgtct
     1741 ccttgtcggg tcgcggcccg ttttttgccg agtataggcc gcatgccggg cgatgcaagc
     1801 cttgcgcgag caaaagtgtc cgcctgttcc aaattgttac acctgttccc gtctgcaacg
     1861 ttccggcaac gattttcctg tcatgccgcg gcacgataaa tcaatgaaat caaacggctg
     1921 taatgctggc acgggccttg atatggaaac gcggccgaca ggcaagccat ttaccgatcc
     1981 gtaaaagacg aggagacaaa atgatctacg cagcccctgg cgccgctggc g
//
LOCUS       DAOSDX010000564         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_18997_length_2029_cov_3.136778, whole genome shotgun sequence.
ACCESSION   DAOSDX010000564 DAOSDX010000000
VERSION     DAOSDX010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18997_length_2029_cov_3.136778"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLN02_11555"
     CDS             complement(<1..1129)
                     /locus_tag="PLN02_11555"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOI53007.1"
                     /translation="MSQSRIPPASLGGLSYLDPAAPEPWASFIEQIERVRPHLGPLER
                     WIETLKHPKRTLIVDIPIERDDGTIAHFEGYRVQHNLSRGPGKGGIRYHPAVTLNEVM
                     ALAGWMTVKNAAIGLPFGGAKGGIRVDPATLSSGELERVTRRFTSEIGIIIGPDRDIP
                     APDVGTNAKIMAMMMDTFSMNRGGTATGVVTGKPVALGGSLGRQEATGRGVYFCAREA
                     GKRQHIAMEGARIVVQGFGNVGGIAARLFRAAGARVIAIADHTATLVNESGIDIPAAQ
                     AWAAEHAGLAGFPGAAPIDIEDFWQLECDYLVPAALEGQITPERAARVRARVVVEGAN
                     GPTTPAADDVLRSRGVLVVPDVLANAGGVTVSYFEWVQDFSS"
     gene            complement(1153..>2029)
                     /locus_tag="PLN02_11560"
     CDS             complement(1153..>2029)
                     /locus_tag="PLN02_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HOI53008.1"
                     /translation="GMGLPTTANYIVVSTLMAPVIVELGAQSGLLVPLIAVHLFVFYF
                     GLMADVTPPVGLASYAAAGIAKSDPIKTGFTAFGYSARTAILPFMFIFNTQLLLIGIT
                     DAFHLILTVVSATLASLMFAAATQGWFLVRNRLHETLLLLLVTFSLFRPGFWMDMIYP
                     PFDEAAPTELTRLVEAAPKDGKLRVWVEGLSLEGQEVTKGVLLSLGAPGKASERLASM
                     GLTMMTLGDQVQVAAVRFGSPAEKLGLEQGFNLTRIEIPHPDRPDKEWIFIPALLLLA
                     LVWFMQNARRRREAA"
BASE COUNT          318 a          634 c          749 g          328 t
ORIGIN      
        1 agctggagaa atcctgcacc cattcgaagt aggaaacggt gacgccgccg gcattggcca
       61 gcacgtcggg gacgaccaga acgccacggc tgcgcagcac gtcgtcggcg gccggcgtgg
      121 tcggcccgtt ggcgccttcg acgacgacgc gggcgcgcac gcgggcggcg cgttccgggg
      181 tgatctggcc ttccagcgcg gccggcacca ggtagtcgca ctccagttgc cagaagtcct
      241 cgatgtcgat cggtgcggca ccggggaagc cggccaggcc cgcgtgttcg gcggcccagg
      301 cctgggcggc ggggatgtcg atgccggact cgttgaccag cgttgccgtg tggtcggcga
      361 tggcgatcac ccgggcgccg gcggcgcgga acaggcgggc ggcgatgccg ccgacgttgc
      421 cgaagccctg gacgacgatg cgcgcgcctt ccatggcgat gtgctggcgc ttgccggcct
      481 cgcgggcgca gaaatagacg ccgcggccgg tggcttcctg gcggccgaga ctgccgccaa
      541 gggcgaccgg cttgccggtg acgacgccgg tggcggtgcc gccgcggttc atcgagaagg
      601 tgtccatcat catcgccatg atcttggcgt tggtgccgac gtcgggcgcc gggatgtcgc
      661 ggtcggggcc gatgatgatg ccgatttccg aggtgaagcg gcgggtcacg cgttccagtt
      721 cgccgctgct cagcgttgcc ggatcgaccc ggatgcctcc cttggcgccg ccgaagggca
      781 gcccgatggc ggcgttcttg acggtcatcc agccggccag cgccatcacc tcgttgagcg
      841 tcaccgccgg gtggtagcgg atgccgccct tgccgggacc gcgcgacagg ttgtgctgca
      901 cgcggtagcc ctcgaaatgg gcgatcgtcc cgtcgtcgcg ttcgatcggg atgtcgacga
      961 tcagcgtccg tttcgggtgc ttcagagttt caatccagcg ctcaagcggg ccgagatggg
     1021 ggcgcacccg ttcgatctgc tcgatgaagc tggcccaggg ctccggcgcg gccgggtcga
     1081 ggtaggagag cccgcccagg ctggcgggcg ggatgcgcga ttggctcatc gggaactcct
     1141 ttcagtgacg gttcaggctg cctcgcgccg gcggcgcgcg ttctgcatga accagaccag
     1201 cgccagcagg agcagcgccg ggatgaagat ccattccttg tccggacgat ccgggtgcgg
     1261 aatctcgatc cgggtcaggt tgaagccctg ttccaggccg agtttttccg ccgggctgcc
     1321 gaagcgtacg gcggcgacct gcacctggtc gcccagcgtc atcatggtca ggcccatgct
     1381 ggccaggcgt tcgctggcct tgcctggggc gccgagcgaa agcagcacgc ccttggtgac
     1441 ttcctggcct tcgaggctga gaccttcgac ccagacgcgc agcttgccat ccttcggcgc
     1501 cgcttcgacg aggcgcgtca gttcggtcgg ggcggcttcg tcgaacggcg gatagatcat
     1561 gtccatccag aaaccggggc ggaacaggct gaaggtgacg agcagcagga gcagggtttc
     1621 gtgcagacga ttgcggacca ggaaccagcc ctgcgtcgcg gcggcgaaca tcaggctggc
     1681 cagcgtcgcg ctgacaacgg tcaggatcag gtggaaggca tcggtgatgc cgatcagcaa
     1741 tagctgggtg ttgaagatga acatgaaggg caggatcgcc gtccgcgcgc tgtagccgaa
     1801 ggcagtgaag ccggtcttga tcgggtcgct cttggcgatg ccggccgccg cgtaggaggc
     1861 caggccgacc ggcggcgtga cgtcggccat cagaccgaag tagaagacga acaggtggac
     1921 ggcgatgagc ggcacgagca ggccgctctg cgcgccgagt tcgacgatca ccggcgccat
     1981 caaggtcgac acgacgatgt agttggcggt ggtcggcagg cccatgccg
//
LOCUS       DAOSDX010000565         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19027_length_2026_cov_2.431761, whole genome shotgun sequence.
ACCESSION   DAOSDX010000565 DAOSDX010000000
VERSION     DAOSDX010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19027_length_2026_cov_2.431761"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLN02_11565"
     CDS             complement(<1..859)
                     /locus_tag="PLN02_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOI53009.1"
                     /translation="MSSPALPARINLALQGGGAHGAFTWGVLDALLEDESIDFEGVSG
                     TSAGAMNAACLAHGLLHGGRDGARRALADFWLAIADTLPAQFSGNQVASMAPALKMML
                     QWTDHLSPEQLNPFDLNPLRDVIERQIDFAALRAGSPVKLFIATTHANSGKLRLFQNH
                     ELSADVLLASACLPTIHRTITIDGEPYWDGGYSANPAVFPLVYHCQSADLMLVLLTPL
                     HYSETPESAKEIRERLRELAFNSTFLREMRMFAHLREQIGDSNPLPDWLLDRLPIARL
                     ERRFARLRFH"
     gene            complement(901..1167)
                     /locus_tag="PLN02_11570"
     CDS             complement(901..1167)
                     /locus_tag="PLN02_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acyltransferase"
                     /protein_id="HOI53010.1"
                     /translation="MAHRTVHMPKYPECWQSCGSCASGDVFILEVLVALGEQLRHDDN
                     ILTLETGKVALDIPTPYAGRVVEVHVSAGDHVAEGAPLVTLECD"
     gene            complement(1179..2015)
                     /locus_tag="PLN02_11575"
     CDS             complement(1179..2015)
                     /locus_tag="PLN02_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOI53011.1"
                     /translation="MLAACVTTQPAPPPAPPTPPPTAHKPKIGLALGGGAARGFAHIG
                     VIKMLESQGIVPDLVVGTSAGAVVGAMYAGGYDAFAMQKLAIQLDETLFADWTLRGPG
                     LLKGEALQSFVNQNLKRRPLEKLKLPFGTVATDLNSGERVVFRTGDSGMAVRASAAVP
                     GVFQPVTINGHQYVDGGLTSPVPVRAAREMGADFVIAVDISAVAEGQPVDSMSAILWQ
                     TTTIMGSVIGKTELAEADAVIRPRLPYVKSWDFNARHEAMIEGEKAALAVLPTIRRKL
                     AR"
BASE COUNT          300 a          633 c          770 g          323 t
ORIGIN      
        1 cgtggaagcg caggcgggca aagcggcgct ccaggcgggc gatcggcagg cggtcgagga
       61 gccagtcggg gagcggattg ctgtcgccga tctgctcgcg caggtgggcg aacatccgca
      121 tctcgcgcag gaaggtcgaa ttgaaggcga gttcgcgcag gcgttcgcgg atttccttgg
      181 cgctttccgg cgtttccgaa tagtgcagcg gtgtcagcag gaccagcatc aggtcggccg
      241 actggcagtg gtagaccagc ggaaagaccg ccggattggc gctgtagccg ccgtcccagt
      301 aaggttcgcc gtcgatcgtg atggtccggt ggatggtcgg caggcaggcc gaggcgagca
      361 gcacgtcggc cgacagttca tggttctgga acaggcgcag cttgccggag ttggcgtggg
      421 tggtggcgat gaacagcttg accgggctgc cggcgcgcag ggcggcgaag tcgatctggc
      481 gctcgatgac gtcgcgcagc gggttgaggt cgaaggggtt caactgctcc gggctgaggt
      541 ggtcggtcca ttgcagcatc atcttcagcg ccggtgccat gctcgccacc tggttgccgg
      601 aaaattgcgc cggcagggtg tcggcgatcg ccagccagaa atcggccagc gcccgccgcg
      661 cgccgtcgcg gccgccgtgc agcaggccgt gggcgagaca ggcggcgttc atggcgccgg
      721 cactggtgcc gctgacgccc tcgaaatcga tcgattcgtc ttcgagcagg gcgtcgagca
      781 cgccccaggt gaaggcgcca tgggcgccgc cgccctgcag ggcgaggttg atccgggcgg
      841 gcagggctgg gctggacatc ggcagggact ccacggtgac gacgccccga tgatagccgc
      901 ttaatcgcac tccagcgtga ccagcggcgc gccttcggcg acgtggtcgc cggcgctgac
      961 atggacttcg acgacgcggc cggcgtaggg cgtcggaatg tcgagcgcga ccttgccggt
     1021 ttccagggtc aggatattgt cgtcgtggcg cagctgttcg cccagcgcga ccagcacttc
     1081 caggatgaag acgtcgccgc tggcgcagga gccgcagctc tgccagcact ccgggtactt
     1141 cggcatgtgg acggtgcggt gcgccacggt cgcccgcttc agcgcgccag cttgcggcgg
     1201 atcgtcggca ggacggcgag cgccgccttt tcgccctcga tcatcgcctc gtggcgggcg
     1261 ttgaaatccc aggacttgac gtagggcagg cgcgggcgga tcaccgcgtc ggcctcggcc
     1321 agctcggtct tgccgatgac gctgcccatg atcgtcgtcg tctgccagag gatggcgctc
     1381 atgctgtcga ccggctggcc ctcggcaacc gccgagatgt cgacggcgat gacgaaatcg
     1441 gcgcccatct cgcgcgcggc gcgcaccggc accgggctgg tcagcccgcc gtcgacgtac
     1501 tggtggccgt tgatcgtcac cggctggaag acgccgggga cggcggccga ggcgcgtacc
     1561 gccatgccgc tgtcgccggt ccggaagacg acgcgctcgc cgctgttgag gtcggtggcg
     1621 acggtgccga agggcagctt gagcttttcc agcgggcgcc gcttcaggtt ctggttgacg
     1681 aaggattgca gcgcctcgcc cttgagcagg ccggggccgc gcagcgtcca gtcggcgaac
     1741 agcgtttcgt cgagctggat cgccagcttc tgcatggcga aggcatcgta gccgccggcg
     1801 tacatcgcgc cgacgacggc gccggcactg gtgccgacga cgagatcggg gacgatgccc
     1861 tgggattcca gcatcttgat cacgccgata tgggcgaagc cgcgcgccgc accgccgccg
     1921 agagcgaggc cgatcttcgg cttgtgggcg gtcggcgggg gcgtcggcgg cgccggcggt
     1981 ggtgccggct gggtggtgac gcaagcggcg agcaggaagg cgccgg
//
LOCUS       DAOSDX010000566         2025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19035_length_2025_cov_3.396954, whole genome shotgun sequence.
ACCESSION   DAOSDX010000566 DAOSDX010000000
VERSION     DAOSDX010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19035_length_2025_cov_3.396954"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLN02_11580"
     CDS             <1..336
                     /locus_tag="PLN02_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOI53012.1"
                     /translation="LAAACGADGVHLSAARLAECKERPDFAWVGASCHTAAEIARAGE
                     LELDYALLGPVMATPTHPEHPGLGWAEFARRGAGNTLPLFALGGMRETLRDSACLHGA
                     HGIALMRGW"
     gene            complement(359..565)
                     /locus_tag="PLN02_11585"
     CDS             complement(359..565)
                     /locus_tag="PLN02_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOI53013.1"
                     /translation="MKPPRLVRCPQCGKESPWSPENRWRPFCSERCKQIDLGAWASES
                     YTIPGAPTDEETAAPGIDFSSTPR"
     gene            complement(562..1320)
                     /gene="zapD"
                     /locus_tag="PLN02_11590"
     CDS             complement(562..1320)
                     /gene="zapD"
                     /locus_tag="PLN02_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004381200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HOI53014.1"
                     /translation="MITYEYPFNERIRTLLRLEDLFEKTAYFARADGQHEHHVALVTL
                     FEILEAAGRADLKMDLIQELERQRQTLLAFRNNPDISEEALSGALYEIEQSSASLLAM
                     SGKIGQYLRDNDWLMSIKSRAAIPGGVCEFDLPSYHWWLHRTPEARRNALEGWSKPLF
                     ALRDASAIVLRLLRSSGRPKRHVASNGQFQLNLGGTSAQMVRVELAAEESAVPEVSAN
                     KYFLNIRFTKPPLNELKARGCERDVEFALTFCNL"
     gene            complement(1394..2002)
                     /gene="coaE"
                     /locus_tag="PLN02_11595"
     CDS             complement(1394..2002)
                     /gene="coaE"
                     /locus_tag="PLN02_11595"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289434.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOI53015.1"
                     /translation="MSDFVVGLTGGIGSGKSTVADAFVELGAALVDTDAIAHELTAPG
                     GAAMPALIAAFGPAIADPSGALDRAAMRRLAFADPGARQRLEGILHPMIRQLSDARCR
                     AASAPYVILAVPLLVESGSYRERCRRILVVDCPESLQIERVMARNGISEAEVRAILAA
                     QASREARLAIADDVVVNDGERTILYRAVAELHAKYLHLAGRT"
BASE COUNT          321 a          658 c          709 g          337 t
ORIGIN      
        1 ctggctgccg cctgcggcgc cgacggcgtg cacctttccg cagcgaggct tgccgagtgc
       61 aaggagcgcc cggatttcgc ctgggtcggc gcgtcctgcc acacggcggc ggaaatcgcc
      121 cgcgccggcg agctggagct ggattacgcg ctactcggcc cggtcatggc gacgccgacc
      181 caccctgaac acccgggact gggctgggcg gaattcgccc gccgcggcgc cggcaatacg
      241 ctgccgctgt ttgcgctggg cggcatgcgc gagacgctgc gcgacagcgc ctgcctgcac
      301 ggcgcccacg gcattgcgct gatgcgcggc tggtaggaaa gaagaagcag cgcgccgttc
      361 agcgcggcgt gctgctgaag tcgatgcccg gcgccgcggt ttcctcgtcg gtcggcgcgc
      421 cggggatggt gtagctttcg ctcgcccagg cgccgagatc gatctgcttg cagcgctcgg
      481 agcagaaggg ccgccagcgg ttctccggcg accacgggga ttccttgccg cactgcgggc
      541 agcgcacgag acggggcggc ttcacaggtt gcagaaggtc agcgcgaatt cgacgtcgcg
      601 ctcgcagccg cgcgccttca gctcgttgag cggcggcttg gtgaagcgga tgttgaggaa
      661 gtacttgttg gcgctgacct cgggcaccgc gctttcctcc gccgccagtt cgacgcgcac
      721 catctgcgcc gaggtaccgc cgaggttgag ctggaactgc ccgttgctgg cgacgtggcg
      781 cttcggccgg ccgctcgagc gcagcagccg gaggacgatc gccgacgcgt cgcgcagcgc
      841 gaacagcggc ttgctccagc cttccagcgc gttgcgccgg gcttccggcg tgcggtgcag
      901 ccaccagtgg taggacggca gatcgaattc gcagacgccg ccgggaatcg ccgcccggct
      961 cttgatgctc atcagccagt cgttgtcgcg caggtactgg ccgatcttgc cgctcatcgc
     1021 cagcaacgag gccgacgact gctcgatctc gtagagcgcg ccggacagcg cctcttcgga
     1081 aatgtccggg ttgttgcgga aggcgagcaa ggtctggcgc tgccgttcga gctcctggat
     1141 caggtccatc ttgaggtcgg cacgaccggc agcctcaagg atttcgaaca gcgtgacgag
     1201 ggctacgtga tgttcgtgct gaccgtcggc acgagcgaaa tacgccgtct tctcgaaaag
     1261 atcttcgaga cgcaacagcg tgcggatgcg ctcgttgaac gggtattcgt aggtgatcac
     1321 tcggccgggt tccggaaatc ggaaaaattt gctgaattct gaggcatttg cgagcaaatc
     1381 tcaacagcca gctttaagtt ctgccggcaa gatgaagata ttttgcatgc agctcggcaa
     1441 ccgcccggta aagaattgtt cgctcgccgt cattgaccac gacgtcgtcg gcaatcgcca
     1501 gccttgcctc acggctggcc tgggcggcga ggatggcgcg cacctcggct tcgctgatgc
     1561 cgttgcgcgc catgacgcgc tcgatctgca ggctttccgg gcaatcgacg accaggatgc
     1621 gccggcaacg ctcgcggtag ctgccggatt cgaccagcag cggcacggcg aggatgacgt
     1681 aaggcgcgct cgccgcccgg cagcgggcat cggatagctg gcggatcatc ggatggagga
     1741 tgccttccag gcgctggcgc gcgccggggt cggcgaaggc cagacggcgc atcgcggcgc
     1801 ggtcgagggc gccggacgga tcggcgatcg ccggcccgaa agcggcgatc agcgccggca
     1861 tcgcggcccc gccgggcgcc gtcaactcgt gggcgatggc atcggtatcg accagcgccg
     1921 cgccgagctc gacgaaggcg tcggcgaccg tgctcttgcc actgccgatg ccgcccgtca
     1981 ggccgacgac gaaatcgctc acgggcagcg cttcggcgcg ttctt
//
LOCUS       DAOSDX010000567         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19079_length_2021_cov_2.253815, whole genome shotgun sequence.
ACCESSION   DAOSDX010000567 DAOSDX010000000
VERSION     DAOSDX010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19079_length_2021_cov_2.253815"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLN02_11600"
     CDS             complement(<1..490)
                     /locus_tag="PLN02_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53016.1"
                     /translation="MSSTRARDVWAERQEERWQNMQEGLRRLRAMPSPKDTAKAHAAS
                     RVEQLKERIKQLRAMLIGLPPEQAKAILRQISTIAKELAGMAKVAGGDSGGAAAGTTG
                     AASASSPPSSESATVEESPERAPAASAAKDAGADKSLRDKLEEVAKQLKALLEQAKAR
                     MRK"
     gene            623..1357
                     /locus_tag="PLN02_11605"
     CDS             623..1357
                     /locus_tag="PLN02_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOI53017.1"
                     /translation="MELVVLALGRSLADLRAGRVWLYLLLPALAAMLLSVTLALWGLG
                     WLAEQLLVLPPMTWLAAWGLVWLATFLAWLGGWLAIFAVAYLCASLLAAIVIMPLLLK
                     HLSETEYRDLAAMGRDSFVAATVNSLGAALIFIVAWLATIPLWLIPGFSLLVPLLLMA
                     WLNRRTFAYDALSLHASDAEWASLRAQHKLPLFLLGLLMAVLAHVPVLGLLVPALAAL
                     SFVHYGLEALRRSRGGAIVSIQGEQA"
     gene            1354..>2021
                     /locus_tag="PLN02_11610"
     CDS             1354..>2021
                     /locus_tag="PLN02_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOI53018.1"
                     /translation="MSRTFGAVIIGDEILSGKRQDKHFAKIAELLGARGLQLSWVEYL
                     GDDRRRLTDTFKRTMAAGDVVFSCGGIGNTPDDHTRQAVAAALGVDLELHPEGFEELK
                     VRFANEEITEQRKLLVTFPRGVRIVPNPFNRIPGFMANDHYFVPGFPQMAHPMIEWAL
                     DSFYRDEFRAVGDKVEKAFLLTGPNAYESALLDLMERIVAAYPSLRLFSLPSLVGEER
                     RHLEL"
BASE COUNT          286 a          676 c          661 g          398 t
ORIGIN      
        1 ccttgcgcat ccgggccttg gcctgttcga gcagtgcctt caactgcttg gccacttcct
       61 ccagtttgtc gcgcaaggac ttgtcggcgc cggcatcctt cgccgccgag gcagccggcg
      121 cacgctccgg gctttcttcg accgtcgccg attcggacga aggaggcgaa ctggcgctgg
      181 cggcgccagt cgtcccggca gcagcgccgc cgctgtcgcc gcccgccacc ttggccatgc
      241 cggcgagttc cttggcgatc gtcgaaatct ggcgaagaat ggccttggcc tgttccggtg
      301 gcaggccgat caacatcgcc cgcaactgct tgattctctc cttcaattgc tcgacccggg
      361 atgcggcgtg cgccttcgcg gtgtcctttg ggctgggcat ggcgcgtagg cggcgcaggc
      421 cttcctgcat gttctgccat cgctcttcct ggcgttctgc ccagacatcg cgggccctgg
      481 tcgaagacaa ggcgatcgtt tggctggcag acaaggacgg caggttcatc ggggcgggaa
      541 aacagaggca atggcagttc tacggcgagg gcggcaaaaa ctttaaaatg acgcctgcgt
      601 tcgctttccg gaaggtgccg tgatggaact cgtggttctg gccttgggcc gctcgttggc
      661 cgacctgcgt gccggtcggg tctggttgta cctgttgttg ccggcattgg ccgccatgct
      721 gctctcggtg acgctggcgc tgtgggggct gggctggctg gccgagcaac tgctcgtgct
      781 gccgccgatg acctggctcg ccgcctgggg gctggtctgg ctggccacct tcctggcctg
      841 gctgggcggc tggctggcga tcttcgccgt cgcctacctg tgtgcctcgc tgctcgcggc
      901 gatcgtcatc atgccgctgt tgctcaagca tctgtcggaa accgagtacc gcgacctggc
      961 cgcgatgggg cgcgacagct tcgtcgccgc cacggtcaac agcctggggg cggcgctgat
     1021 cttcatcgtc gcctggctgg cgaccatccc gctgtggctg attcccggct tctcactgct
     1081 cgttccgttg ctgctgatgg cctggctcaa ccggcgcacc ttcgcctacg acgcgctgtc
     1141 gctgcacgcg agcgacgccg aatgggccag cctgcgggcg cagcacaagc tgccgctgtt
     1201 cctgctcggc ctgctcatgg cggtgctcgc ccacgtgccg gtgctcggcc tcctggtgcc
     1261 ggcgctggct gcgctatcat tcgttcatta cgggctggaa gccctgcgcc gctcgcgcgg
     1321 tggcgccatc gtcagcattc agggagaaca agcatgagtc gaaccttcgg cgccgtcatc
     1381 atcggcgacg aaatcctgtc cggcaaacgc caggacaagc acttcgcgaa gatcgccgag
     1441 ctgctcggcg cgcgcggcct gcaactgtcc tgggtcgaat atctgggcga cgaccggcgg
     1501 cgcctgaccg acaccttcaa gcgcacgatg gctgccggtg acgtcgtctt ttcctgcggc
     1561 ggcatcggca acacgcccga tgaccacacc cggcaggcgg tggcggcggc gctcggcgtc
     1621 gatcttgaac tgcacccgga aggtttcgag gaactgaagg tgcgtttcgc caacgaggaa
     1681 atcaccgaac agcgcaagct gctggtcacc ttcccgcgcg gcgtgcgcat cgtgcccaac
     1741 ccgttcaacc gcatcccggg tttcatggcc aacgaccact atttcgtgcc tggcttcccg
     1801 cagatggcac atccgatgat cgagtgggcg ctcgacagct tctaccggga cgaattcagg
     1861 gcggtcggcg acaaagtcga gaaggccttc ctgctgaccg ggccgaacgc ctacgagagc
     1921 gcgctgctcg acctgatgga gcggatcgtc gccgcctacc cgagcctgcg cctgttctcg
     1981 ctgccctcgc tggtcggcga ggaacgccgg cacctggagc t
//
LOCUS       DAOSDX010000568         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19226_length_2009_cov_4.263050, whole genome shotgun sequence.
ACCESSION   DAOSDX010000568 DAOSDX010000000
VERSION     DAOSDX010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19226_length_2009_cov_4.263050"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="PLN02_11615"
     CDS             <1..177
                     /locus_tag="PLN02_11615"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285859.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOI53019.1"
                     /translation="PNAQAAAKDLIRAVDHQPVNQTLVEETAHRIAHLRATPEAREGI
                     AAFLEKRQPNWMGE"
     gene            180..305
                     /locus_tag="PLN02_11620"
     CDS             180..305
                     /locus_tag="PLN02_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53020.1"
                     /translation="MFTKILIANRGDQPRSGAATKSNCLRAQHAQAISPRETTHV"
     gene            298..>2009
                     /locus_tag="PLN02_11625"
     CDS             298..>2009
                     /locus_tag="PLN02_11625"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOI53021.1"
                     /translation="MFNKILIANRGEIACRVIKTARRMGIRTVAVYSEADANARHVRM
                     ADEAVLLGPAAARESYLVADKIIEACQRTGAQAVHPGYGFLSENEEFAEALAQAGLVF
                     IGPPASAIRAMGSKSEAKKLMGTAKVPLTPGYHGDDQRPTLLHAEAEKIGYPVLIKAA
                     AGGGGKGMRLVEKSEDFPDALASCKREALSSFGDDHVLIEKYITKPRHVEIQVFADTL
                     GNCVYLFERDCSVQRRHQKVLEEAPAPGMREARRREMGEAAVAAAKAVGYVGAGTVEF
                     IANQDGSFFFMEMNTRLQVEHPVTEMITGQDLVEWQLRVAAGQPLPLKQEQLEIRGHA
                     LEARIYAEDASKGFLPATGKLVRLVPPAESINVRVDTGVEEGDEITPFYDPMIAKLIV
                     WDGTRDGALARMRKALADYRVAGLTTNIDFLSRLVACPAFAKADLDTGLIERQKDFLF
                     PAAQPVPRDALLVATVGELLWEQHAAKQAAQHSGDPFSPWHARDGWRMNLSAARTISF
                     MDGETLVDVQVRYRGERWEIALCGESTVASGKKLEGDRFAVELDDRRLIASVVAVDDK
                     RTVFL"
BASE COUNT          369 a          702 c          647 g          291 t
ORIGIN      
        1 cccaacgcgc aggccgccgc caaggacctg atccgcgccg tcgatcacca gccggtcaac
       61 cagaccctgg tcgaggaaac cgcccaccgc atcgcccatt tgcgggcaac gccggaggca
      121 cgcgagggca ttgcggcgtt tctcgaaaag cgccaaccga actggatggg ggaatgagca
      181 tgttcaccaa gattctgatt gccaaccgcg gcgaccagcc gcgcagcggc gcagcgacga
      241 agtcaaactg cctgcgtgcg cagcacgctc aggcgatttc tccgcgggag accacgcatg
      301 tttaacaaga tactgatcgc caaccgcgga gaaatcgcct gccgggtgat taaaaccgcc
      361 cgccgcatgg gcatccgtac cgtcgccgtc tattccgagg ccgacgccaa tgcccgccac
      421 gtgcgcatgg ccgacgaggc cgtgctgctc gggccggcgg cggcgcgcga gtcctatctg
      481 gtggcagaca agatcatcga ggcctgccag cgcaccggcg cccaggcggt gcatccgggc
      541 tacggttttc tctctgaaaa cgaggagttc gccgaagccc tggcccaggc cggcctcgtc
      601 ttcatcggcc cgccggcatc ggcgatccgc gccatgggct cgaagtcgga agccaagaaa
      661 ctgatgggca cggccaaggt gccgctgacg cccggctacc acggcgacga ccagcgcccg
      721 acgctgctcc acgccgaggc cgagaagatc ggctacccgg tgctgatcaa ggctgcggcc
      781 ggcggtggcg gcaagggcat gcgcctggtc gagaagtccg aggatttccc cgacgcgctg
      841 gcttcctgca agcgcgaggc gctgtcgagc ttcggcgacg accatgtgct catcgagaaa
      901 tacatcacca agccgcgcca cgtcgaaatc caggtcttcg ccgacacgct gggcaactgc
      961 gtctatctgt tcgagcgcga ctgctcggtg cagcgccgcc accagaaggt gctggaagaa
     1021 gccccggcac cgggcatgcg cgaagcccgc cgccgcgaga tgggcgaagc ggcggtggcc
     1081 gccgccaagg ccgtcggcta cgttggcgcc ggcaccgtcg agttcatcgc caaccaggac
     1141 ggcagcttct tcttcatgga gatgaacacc cgcctgcagg tcgagcaccc ggttaccgag
     1201 atgatcaccg gccaggatct ggtcgaatgg cagttgcgcg tcgccgccgg ccagccgttg
     1261 ccgctgaagc aggaacaact ggaaatccgc ggccacgcgc tggaggcgcg catctacgcc
     1321 gaggacgcca gcaagggctt cctgcccgcc accggcaagc tggtgcgcct ggtgccgccg
     1381 gctgaatcga tcaacgtccg cgtcgatacc ggcgtcgagg aaggcgacga aatcacgccc
     1441 ttctacgacc cgatgatcgc caagctgatc gtctgggacg gaacccgcga cggcgccctg
     1501 gcgcgcatgc gcaaggcgct ggccgactac cgcgtcgccg gcctgaccac caacatcgat
     1561 ttcctctccc gcctggtcgc ctgcccggcc ttcgccaagg ccgatctgga caccggcctg
     1621 atcgaacggc agaaggattt cctcttcccc gccgctcagc cggtgccgcg cgacgcactg
     1681 ctggtcgcca cggtcggcga actgctctgg gaacagcacg cggcaaagca ggccgcgcag
     1741 cacagcggcg acccgttctc gccctggcac gcacgcgacg gctggcgcat gaacctgtcg
     1801 gccgcgcgca cgatcagctt catggacggc gagacgctgg tcgacgtaca ggtccgctat
     1861 cgcggcgagc gctgggaaat tgccctttgc ggcgagtcta ccgttgccag cggcaagaag
     1921 ctggaaggcg accgcttcgc cgtcgaactc gacgaccgcc gactgatcgc ctcggtggtc
     1981 gcggtcgacg acaagcgcac ggtctttct
//
LOCUS       DAOSDX010000569         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19292_length_2005_cov_2.769231, whole genome shotgun sequence.
ACCESSION   DAOSDX010000569 DAOSDX010000000
VERSION     DAOSDX010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19292_length_2005_cov_2.769231"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLN02_11630"
     CDS             <1..712
                     /locus_tag="PLN02_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI53022.1"
                     /translation="DCFKFTQNLAGLAAGLGVDFRFGVDIGGLEHDGRRISGVRTTRG
                     TLTADHYVLALGSYSTALLRPLGIDIPVYPVKGFSLTVPIVDPAAAPESTVMDETHKV
                     AVTRLGDRIRVGGTAQLSGFDLSLAEGRRRTLEFVVGDLFPGGGDLARAEFWTGLRPM
                     TPDGTPIVGRTRYDNLLLSTGHGTLGWTMAAGTGRVVADLVCGRQPEIAVDDLGVARY
                     AGAQLRRPAPRPVSAAPA"
     gene            807..1241
                     /locus_tag="PLN02_11635"
     CDS             807..1241
                     /locus_tag="PLN02_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI53023.1"
                     /translation="MPNRAVIKIVQNREFLVCQPADDVRTVAAHMKRHGQAAVLVVSP
                     EGLLLGICTERDLAYKVLAEGLDGTTPVSAVMTPEPRTVPPEMIFGHVLHLMYEGGFR
                     HMPVVDPGGRPLGLVSARDALGLEMISFAGEMAQREALAEVL"
     gene            complement(1238..>2005)
                     /locus_tag="PLN02_11640"
     CDS             complement(1238..>2005)
                     /locus_tag="PLN02_11640"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI53024.1"
                     /translation="TLGLPLFEQIGRDIRLTAAGEELLATVRVLDHEWNRLDAAIGAL
                     KGLHSGRLRVALVNTAQYFLPRMLGAFCRRYPEIDVRLEITNRAGVVDRMRKNEDDLY
                     IMSYPPQDIDVVVRPFLDNPLHVVAPLGHRLAGRRIDAAALADEPFLLREAGSGSRAA
                     VDRHLQASGQTLKVRLTVASNEAIRDLVASGVGLSILSRHALAADPADNGLCLLDVAG
                     FPIQQPWSIVHLKGKVLSLPAQAFIGELLQAAAPAAA"
BASE COUNT          299 a          662 c          732 g          312 t
ORIGIN      
        1 cgactgtttc aagttcacgc agaacctggc cgggctggcc gccggactgg gcgtcgactt
       61 ccgcttcggc gtcgatatcg gcgggctgga gcacgacggg cggcggattt ccggcgtccg
      121 tacgacgcgc gggacgctga cggcggacca ttacgtgctg gccctgggca gttattcgac
      181 cgccctgctg cggccgctcg gtatcgacat cccggtctat ccggtcaagg gtttctcgct
      241 caccgtgccg atcgtcgatc cggcggcggc accggagtcg acggtcatgg acgaaaccca
      301 caaggtggcg gtgacccggc tcggcgaccg catccgcgtc ggcggcacgg cgcagctttc
      361 cggcttcgac ctcagcctgg ccgaagggcg gcggcggacg ctggaattcg tcgtcggcga
      421 cctcttccct ggcggcggcg acctcgcccg ggccgagttc tggaccggcc tgcggccgat
      481 gacgccggac ggcacgccga tcgtcggccg cacccgttac gacaacctgc tgctgagcac
      541 cggccacggc acgctgggat ggacgatggc ggccggcacc gggcgcgtcg tcgccgatct
      601 ggtctgtggc cggcagccgg aaatcgccgt cgacgacctc ggcgtcgcgc gttacgccgg
      661 tgctcagcta cgtcgtccgg caccgcgccc ggtttctgcg gcgccggcct gaaacgttac
      721 aaatcggccg ccaattcgct gcaacttctg cgtcggcaac cggtcagtcc tctggtagtg
      781 caacccacca ttacgaggag acgaagatgc cgaacagagc tgtcatcaag attgtccaga
      841 atcgcgaatt cctggtctgc cagccggccg acgacgtgcg gaccgtcgcc gcgcacatga
      901 aacggcacgg ccaggcggcg gtcctggtgg tgtcgccgga gggcctgctg ctcggcatct
      961 gcaccgagcg cgacctggcc tacaaggtgc tggccgaggg gctggacggg acgaccccgg
     1021 tgtcggcggt gatgacgccg gagccgcgca ccgtgccgcc ggagatgatc ttcggccatg
     1081 tcctgcactt gatgtacgaa ggcggtttcc gccacatgcc ggtggtcgat ccgggcggcc
     1141 ggccgctcgg cctggtctcg gcgcgtgacg cgctgggcct ggaaatgatc agcttcgccg
     1201 gcgagatggc gcagcgcgag gcgctggcgg aagttctcta ggctgctgca ggcgccgccg
     1261 cctgcagcag ttcgccgatg aaggcctggg cgggcaggga gagcaccttg cccttcagat
     1321 gcacgatcga ccacggctgc tggatcggaa agccggcgac gtcgagcagg cagagaccgt
     1381 tgtcggccgg atcggcggcc agcgcgtggc gcgacaggat ggacaggccg acgccgctgg
     1441 cgacgaggtc gcggatcgcc tcgttgctgg cgaccgtcag cctgaccttg agcgtctggc
     1501 cgctcgcctg cagatgacgg tccaccgccg cccgcgaacc ggagccggct tcgcgcagca
     1561 gaaagggctc gtcggccagg gcggcggcgt cgatccgccg gccggccagg cgatggccga
     1621 gcggggcgac gacgtgcagc ggattgtcga ggaaggggcg gacgacgacg tcgatgtcct
     1681 gcggcgggta ggacatgatg tagaggtcgt cctcgttctt gcgcatccgg tcgacgacgc
     1741 cggcgcggtt ggtgatctcg agccggacgt cgatttccgg gtagcggcgg cagaaggcgc
     1801 cgagcatgcg cggcaggaag tactgcgcgg tattgaccag ggcaacgcgc aggcgcccgc
     1861 tgtgcaggcc tttcagcgcg ccgatcgcag cgtcgaggcg gttccattcg tggtcgagga
     1921 cgcgcaccgt cgccagcagt tcctcgccgg cggcggtcag ccggatgtcg cggccgatct
     1981 gctcgaacag cggcagaccc agcgt
//
LOCUS       DAOSDX010000570         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19299_length_2004_cov_2.779374, whole genome shotgun sequence.
ACCESSION   DAOSDX010000570 DAOSDX010000000
VERSION     DAOSDX010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19299_length_2004_cov_2.779374"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLN02_11645"
     CDS             complement(<1..930)
                     /locus_tag="PLN02_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOI53025.1"
                     /translation="MQQQSTVQVMGIDAGGTMTDTFFVRADGRFVVGKAQSNPGDESL
                     AIFNSSQDALAHWNRSVDDVYPELATCVYSGTAMLNRILMRKGLDVGLICNKGFEQIH
                     SMGRALQSYLGYALEDRIHLNTHRYDDPLVPVSRTRGVTERTDVQGKVVIPLREDEVR
                     QAIRELVEAGSQAIVICLLQSHKNESSEQRARDVARDELQKLKVDIPVFASVDYYPSR
                     KESHRMNTTILEAYGAEPSRQTLKKVSDRFKKHGAKFDLRVMATHGGTISWKAKELAR
                     TIVSGPIGGVIGSKLLGEALGDENIACSDIGGTS"
     gene            1291..>2004
                     /locus_tag="PLN02_11650"
     CDS             1291..>2004
                     /locus_tag="PLN02_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOI53026.1"
                     /translation="MPFSQCGSLVPRIGDENVVATAWERFLQDRPWSGGGVRDVVLDS
                     WQRCRSEAVDPALRSAPGAASERLRQLQWQNRDLCAAARPVLDGLRDILRECGTLIML
                     CDQSGTIIDLNGETRARSAGEDINLATGGCWRESVIGTNAIGTAIATAAPVQIYASED
                     FCMDVKRWTCAAAPILDPLARTLLGVVDVSGVKESFHGHTLALVIAAAKQIEGELARH
                     DGLLHGRLLARAIDDFSRYA"
BASE COUNT          328 a          665 c          642 g          369 t
ORIGIN      
        1 gctggtgccg ccgatgtcgg aacaggcgat gttctcgtcg ccgagcgcct cgcccagcaa
       61 cttggagccg atgacgccgc cgatcgggcc ggagacgatg gtgcgcgcca gttccttggc
      121 cttccagctg atcgtcccgc cgtgcgtggc catcacccgc aggtcgaact tggcgccgtg
      181 cttcttgaag cggtcgctga ccttcttcag cgtctggcgc gacggttcgg cgccgtaggc
      241 ttcaaggatg gtggtgttca tccggtggct ttccttgcgc gacgggtagt aatcgaccga
      301 ggcgaagacc gggatatcga ccttgagctt ctgcagttcg tccctcgcca cgtcgcgggc
      361 gcgctgctcg ctgctctcgt tcttgtgcga ctggagcagg cagatgacga tggcttgcga
      421 gccggcttcg accagttcgc ggatggcctg gcgcacttcg tcctcgcgca gcggaatgac
      481 caccttgccc tgcacgtcgg tgcgctcggt gacgccgcgg gtgcgcgaaa ccggcaccag
      541 ggggtcgtcg tagcggtggg tgttgaggtg gatgcgatct tccagcgcgt agccgaggta
      601 gctctgcagg gcgcggccca tcgagtgaat ctgctcgaaa cccttgttgc agatcaggcc
      661 gacgtcgagc cccttgcgca tcaggatgcg gttcaacatc gcggtaccgg aatagacgca
      721 ggtcgccagt tccggataga cgtcgtcgac gctgcggttc cagtgcgcca gcgcgtcctg
      781 cgacgagttg aagatggcga gcgactcgtc gcccgggttg ctttgcgcct tgccgacgac
      841 gaaacggccg tcggcgcgga caaagaaggt gtcggtcatc gtgccgccgg catcgattcc
      901 catcacctgg acggtggatt gttgttgcat gttgcctcct gtatttggtg tgtgcatggg
      961 gggcttcccc acgccagctt cttggcaagg gccgggccag gcctcggaga cggtatgcca
     1021 cctagcaaaa atccgtttgc gaatcagtgg cttgaaagta ccaaccggat gccgcccagg
     1081 ccgcctcgcc gactgtccgc cactgtccgt tttaacgacg aacggacgcc gctgtccgga
     1141 atccagacac ttttccggcc ccgtgctgcg ccgcacaagc cattgatcca taaggaattt
     1201 attgacacat cccgacaagc cgactagggt tacgcaatgg tttatcgctt cacctaatca
     1261 aaaaccaaca gccaacgaga tcgacgcccc atgccctttt cccaatgcgg ctcactggtt
     1321 ccccggattg gcgacgagaa cgtcgtcgcc accgcctggg aacgtttcct gcaggatcgg
     1381 ccgtggtccg gcggcggcgt ccgcgacgtg gtcctcgatt cctggcagcg ctgccgctcg
     1441 gaagccgttg acccggcgct gcgcagcgcg ccgggcgcgg ccagtgaacg cctgcgccag
     1501 ttgcaatggc agaaccgcga cctgtgcgcg gcggcccgcc cggtgctcga cggcttgcgc
     1561 gacatcctgc gcgaatgcgg caccctgatc atgctgtgcg accagagcgg caccatcatc
     1621 gacctcaacg gcgaaacccg ggcgcgcagt gccggcgagg acatcaacct ggcgaccggc
     1681 ggttgctggc gcgaatcggt gatcggcacc aacgccatcg gcaccgcgat cgccaccgcc
     1741 gccccggtgc agatctacgc cagcgaagat ttctgcatgg acgtcaagcg ctggacctgc
     1801 gccgcggcgc ccatcctcga cccactggcg cgcaccctgc tcggcgtggt cgacgtctcc
     1861 ggcgtcaagg aaagcttcca cggccacacc ctggccctgg tgatagccgc cgccaagcag
     1921 atcgagggcg aactggcgcg ccacgacggc ctgctgcacg gccgcctgct ggcccgggcg
     1981 atcgacgatt tttcccgcta cgcg
//
LOCUS       DAOSDX010000571         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19326_length_2002_cov_2.645609, whole genome shotgun sequence.
ACCESSION   DAOSDX010000571 DAOSDX010000000
VERSION     DAOSDX010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19326_length_2002_cov_2.645609"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLN02_11655"
     CDS             <1..280
                     /locus_tag="PLN02_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOI53027.1"
                     /translation="NLAFHDRIVEMTGNAALLDVYRRLVKQLALFRRRNLLAPRAIPH
                     FADEHSAIVDRIAAGDAAGAAEALHAHARGGRERMLRDGELGEVSDAA"
     gene            270..1601
                     /locus_tag="PLN02_11660"
     CDS             270..1601
                     /locus_tag="PLN02_11660"
                     /inference="COORDINATES: protein motif:HMM:NF004755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOI53028.1"
                     /translation="MPPEADRSEGDVNLGARRAAWQEEHLDAATRELLAEDARWFLHQ
                     SMSTPCLNALAGAQGSWLTDLGGRRILDFHGNSLHQVGYGHPKVVAAIQETLATLPFS
                     PRRYANRPAVELARRLCEAAPMHGARLLFAPGGTAAIGMALKLARLATGRFKTLSMWD
                     AFHGASLDAISLGGEAVFRRGIGPLLPGAEHVPPHDPGNCVFGCGGRCALRCVDYIDY
                     CLAKEGDVAAVVVETVRCTDVQVPPADYYQRLRRICDRHGALLILDEIPIALGRTGHL
                     YAVERYGIEPDMIVIGKGLGGGALPLAALIARAGLNVGQNISLGHYTHEKNPLACAAG
                     LATLDVIAEENLLERSRTLGAAALAQLRAATADLPAVVEVRGAGLLLGIELNDADLAE
                     AVLYRCLSQGLSFKVGQGKVLVLAPPLNVDAADLDWALAGVVAAVRAESGG"
     gene            1609..>2002
                     /locus_tag="PLN02_11665"
     CDS             1609..>2002
                     /locus_tag="PLN02_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53029.1"
                     /translation="MPLHVLLAVLAAALLHAGWNALIRGAGDKGLYTVLLHASSGFLA
                     IFALFFLGLPAPASWPYVGASALLHTLYIAWLMRAYDGGQLAVSYTLMRGLPPLLVAL
                     VSAPLLGEPLGSAGWLGIAAICGGVLGIG"
BASE COUNT          256 a          756 c          688 g          302 t
ORIGIN      
        1 caacctcgcc tttcacgacc gcatcgtcga gatgaccggc aacgccgcct tgctcgacgt
       61 ctatcgccgg ctggtcaagc aactggcgct gttccgccgg cgcaacctgc tggcgccgcg
      121 ggcgatcccg catttcgccg acgagcacag cgcgatcgtc gaccgcatcg ccgccggcga
      181 cgctgccggc gccgccgaag cgctgcacgc gcatgcccgc ggcggacggg agcgcatgct
      241 gcgcgacggc gaactcggcg aggtctccga tgccgcctga agccgaccgc agcgaaggcg
      301 acgtcaacct cggcgcccgc cgtgcggcct ggcaggagga gcatctcgac gcggcgacgc
      361 gcgagctgct ggccgaagac gcgcgctggt tcctgcacca gtcgatgtcc acgccctgcc
      421 tcaacgcact ggccggcgcc cagggcagtt ggctgaccga cctcggcggc cggcgcatcc
      481 tcgatttcca cggcaacagc ctgcaccagg tcggctacgg ccacccgaag gtggtcgcgg
      541 cgatccagga aacgctggcg accttgccct tttcgccgcg ccgctacgcc aaccggccgg
      601 ccgtcgaact ggcccgccgc ctctgtgaag cggcgccgat gcacggcgcg cggctgctgt
      661 tcgcgcccgg tggcacggcc gccatcggca tggcgctcaa gctggcccgg ctggcgaccg
      721 gccggttcaa gacgctgtcg atgtgggacg ccttccacgg cgcctcgctc gatgccatct
      781 cgctcggcgg cgaagccgtc ttccgccgcg gcatcggccc gctgctgccc ggcgccgagc
      841 acgtgccgcc gcacgacccg ggcaactgcg ttttcggttg cggcggccgg tgcgcgctgc
      901 gctgcgtcga ttacatcgac tactgtctgg ccaaggaagg cgacgttgcc gccgtcgtcg
      961 tcgaaaccgt gcgctgcacc gacgtccagg tgccgcccgc cgattactac cagcgcctgc
     1021 gccgcatctg cgaccgccac ggcgcgctgt tgatcctcga cgagattccc atcgccctcg
     1081 gccgcaccgg ccacctctac gccgtcgagc gctacggcat cgaaccggac atgatcgtca
     1141 tcggcaaggg cctgggcggc ggcgccctgc cgctcgccgc gctgatcgcc cgcgccgggc
     1201 tcaacgtcgg ccagaacatc tcgctcggcc actacacgca cgagaagaat ccgctcgcct
     1261 gcgccgccgg gctggcgacg ctcgacgtga tcgccgagga aaacctgctc gaacgcagcc
     1321 gcacgctggg cgccgcggcg ctggcgcaat tgcgcgcggc gaccgccgac ctgccggcgg
     1381 tggtcgaagt gcgcggcgcc ggtctcttgc tcggcatcga gctgaacgac gccgacctcg
     1441 ccgaagcggt gctctaccgc tgcctgagcc aaggcctgtc gttcaaggtc ggccagggca
     1501 aggtgctggt gctggcgccg ccgctcaacg tcgacgccgc cgacctcgac tgggcgctgg
     1561 ccggcgtggt cgccgccgtg cgggcggaaa gcggtggctg aggcgggttt gccgctccat
     1621 gtcctgctcg ccgtgctggc ggcggcgctg ctccatgccg gctggaacgc gctgatccgc
     1681 ggcgctggcg acaaggggct gtacaccgtg ctgctgcacg ccagcagcgg cttcctggcg
     1741 atcttcgcgc tgttcttcct gggcctgccg gcgccggcca gctggcctta cgtcggcgcc
     1801 tcggcgctgc tccacacgct gtacatcgcc tggctgatgc gcgcctacga cggcggccag
     1861 ctggcggtca gctacacgct gatgcgcggc ctgccgccct tgctggtggc cctggtctcg
     1921 gcaccgctgc tcggcgagcc gctgggcagc gccggctggc tggggatcgc ggcgatctgc
     1981 ggcggcgtcc tcggcatcgg tt
//
LOCUS       DAOSDX010000572         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19348_length_2000_cov_2.766067, whole genome shotgun sequence.
ACCESSION   DAOSDX010000572 DAOSDX010000000
VERSION     DAOSDX010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19348_length_2000_cov_2.766067"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLN02_11670"
     CDS             <1..462
                     /locus_tag="PLN02_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOI53030.1"
                     /translation="SGNVSNFVMNDKCAAGTGRFLDVAARNLDLDLEDLGDIHFTGSG
                     APLSINSTCTVFAESEIIGLLANGHGKAEIVAGIHYSIAKRTVRLAKRVGIEDRVYMD
                     GGPALNKGLVAAIQDELQRELVVPQYPQTTTAYGAAVLARSEFLAEQQEGH"
     gene            465..1166
                     /locus_tag="PLN02_11675"
     CDS             465..1166
                     /locus_tag="PLN02_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOI53031.1"
                     /translation="MPVEAVAIGLPMALGLGLLYGLGPCLLSCLPYLGPLFVNADQGI
                     ARSWRILLPVSLGRLTVYGGFGAVSGWWGGRYVGGVNGWVVHVTVGVALVLVGLALLG
                     RRRPACGSCAPKNEQKISWRPRQAAAQAMPSGLYLMGVGMALTPCAPQGAVLVAAAAS
                     GTAWSGAALGLSFGLGAIAAPWLVYGTVVAYVGQQLREQLGRWQPRIEALSAFLLLGT
                     GLVQLWQGASAGGMV"
     gene            1186..>2000
                     /locus_tag="PLN02_11680"
     CDS             1186..>2000
                     /locus_tag="PLN02_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOI53032.1"
                     /translation="MNKAWMAGLLAVLAGQGWAAEQVLSTVTVTADADALEERRQAVT
                     QKVILNQAEIANLGATTVSEVISKLPGIDAGSQGSDGGQGMRARGMVRDSVQLLIDGE
                     RVGGNSRMAAASVGRLPSGELERVEILRGSSAEFGGGASVTVNLVMKKTAKKSDTTVK
                     LATGTRDGRVFGQSTVTRRGNAGEFSWVLPLTINHHVMVSGSDSDRQAFTGGARNAWS
                     RDENDGRFTMNELVFSPRLNWRRDGDSFSIWPTLFRGDTRRWGNMDRYSYADPL"
BASE COUNT          318 a          660 c          717 g          305 t
ORIGIN      
        1 agcggcaacg tcagcaactt cgtgatgaac gacaaatgcg ccgccggcac cggccgtttc
       61 ctcgacgtcg ccgcgcgcaa cctcgatctc gacctggaag acctgggcga catccacttc
      121 accggcagcg gcgcgccgct gtcgatcaac agcacctgca ccgtctttgc cgaatccgag
      181 atcatcggcc tgctcgccaa cggccacggc aaggccgaga tcgtcgccgg catccactat
      241 tccatcgcca agcgcacggt gcgcctggcc aagcgggtcg gcatcgagga ccgcgtgtac
      301 atggacggcg gcccggcgct caacaagggg ctggtggcgg cgatccagga cgagctgcag
      361 cgcgagctgg tggtgccgca atacccgcag acgaccaccg cttacggtgc cgccgtgctg
      421 gcgcgcagcg agttcctcgc cgaacagcag gaaggccact gacgatgccg gtcgaggcgg
      481 tggccatcgg cttgccgatg gcgctcgggc tggggctgct ctatgggctc ggcccctgtc
      541 tgctcagttg cctgccttac ctcgggccgc tcttcgtcaa cgccgaccag ggcatcgccc
      601 gttcctggcg tatcctgctg ccggtttcgc ttggccggct caccgtctat ggcggcttcg
      661 gcgcggtcag cggctggtgg ggcggccgct acgtcggcgg ggtgaatggc tgggtggtgc
      721 atgtgacggt cggcgttgcc ctggtgctgg tcggtctcgc gctgctcggc cggcggcgcc
      781 cggcttgcgg cagttgcgcg ccaaagaatg aacagaaaat cagctggcgg ccgcgccagg
      841 cggcggcgca ggcgatgccg tccgggctct atctgatggg cgtcggcatg gcgctgacgc
      901 cgtgcgcgcc gcagggcgcg gtgctggtcg ccgcagcggc cagcggcacg gcgtggagcg
      961 gtgccgcgct cggcctgagc tttggcctcg gcgccatcgc tgcgccatgg ctggtgtatg
     1021 gcaccgtcgt tgcgtacgtc ggccagcaac tgcgcgaaca actggggcgc tggcaaccgc
     1081 gcatcgaagc attgagcgcg ttcctgctgc tgggaacggg cctcgtccag ctctggcaag
     1141 gggccagcgc tggcggcatg gtttgaaacg ggagaagaga aaaacatgaa caaggcttgg
     1201 atggctggcc tgctggccgt gctggcaggg cagggctggg cggcggaaca ggtgttgtcg
     1261 acggtcaccg tgaccgccga cgccgacgcg ctggaagagc gacgccaggc ggtgacgcag
     1321 aaggtgatcc tcaaccaggc ggaaatcgcc aatctcggcg cgacgacggt cagcgaggtg
     1381 atcagcaagc tgcccggcat cgacgccggc agccagggca gcgacggcgg ccagggcatg
     1441 cgggcgcgcg gcatggtgcg cgactcggtg caactgctga tcgacggcga acgggtcggc
     1501 ggcaattcgc ggatggcggc ggcctcggtc ggccgcctgc cgtccggcga actggagcgg
     1561 gtcgagatcc tgcgcgggtc gtcggccgaa ttcggcggcg gcgcctcggt caccgtcaat
     1621 ctggtgatga agaagacggc gaagaagtcc gacacgaccg tcaagctcgc caccggcacc
     1681 cgcgacggtc gggtcttcgg ccagagcaca gtaacccggc gcggcaacgc cggcgagttc
     1741 tcctgggtgc tgccgctgac catcaaccat cacgtcatgg tctccggcag cgacagcgac
     1801 cgccaggcct tcaccggcgg cgcccgcaac gcctggtcac gggacgagaa cgacggccgc
     1861 ttcaccatga acgagctggt gttcagccca cgcctcaact ggcggcgcga cggggatagt
     1921 ttctcgatct ggccgacgct gtttcgcggc gacacccgac gctggggcaa catggaccgc
     1981 tacagctacg ccgacccgct
//
LOCUS       DAOSDX010000573         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19374_length_1998_cov_2.734946, whole genome shotgun sequence.
ACCESSION   DAOSDX010000573 DAOSDX010000000
VERSION     DAOSDX010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19374_length_1998_cov_2.734946"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLN02_11685"
     CDS             <1..371
                     /locus_tag="PLN02_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI53033.1"
                     /translation="TPGEIRTLSNQARVVQVRGEYLPVLVLHEMFKLQSVWNDFTQGI
                     MVVLDADGIRVALFVDALLGQHQVVIKSLEANYRKVPGISGATIMGDGHVALILDVSA
                     IATMARNHLHKADAGPLRSE"
     gene            371..871
                     /locus_tag="PLN02_11690"
     CDS             371..871
                     /locus_tag="PLN02_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOI53034.1"
                     /translation="MEITKQQAGALAEDDSVASEFLTFTLGCEEYAIDILKVQEIRSY
                     ETPTLIANAPPFIKGVVNLRGTIVPIVDLRIKFNLGKIEYTPFTVVVILNIARRVIGV
                     VVDSVSDVLSLTSSQIRPAPDFSGSFDTRYILGLASVEQRMLIVTDIERLMSSADMEL
                     VEAAVE"
     gene            969..>1998
                     /locus_tag="PLN02_11695"
     CDS             969..>1998
                     /locus_tag="PLN02_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI53035.1"
                     /translation="MIAFVVVALISLGMIGEKVQTIAVDNAQGMALASAMRRDVDSLG
                     LHARNALLFEELLRQREAAQGVTDSLAQLQLLEKQLGANLPPAARDVMERLPALREDA
                     AVSLKQLFALLAEGNRPVLESHFENHLGPRLRAWQEAIDTLNLAQKAETSSALEEIAT
                     IRSTVQNLLFGLIVLAVVVMIPSGIWVTRGITNPLSAAVDVAESVAAGNFDNRIEPGG
                     DDEPAHLLRTLARMQADLKERSASERRIAAEALRIKVALDVGSNCVMLLGPDGRIIYG
                     NDALFGMMREAEADIQQQLPEFRAAAIRSADFSVRELNPALAGEQLGALVEPQRSVIR
                     IANRTFALV"
BASE COUNT          378 a          590 c          645 g          385 t
ORIGIN      
        1 cgacgcccgg tgaaatccgt accctgtcga atcaggcgcg ggtggtgcag gtacgcggcg
       61 aatatctgcc ggttctcgtc ctgcacgaaa tgttcaagct gcagtcggtg tggaacgact
      121 tcacccaggg catcatggtg gtgctcgatg ccgacggaat ccgcgttgcc ctgttcgtcg
      181 atgccctgct cgggcagcac caggtggtga tcaagagtct cgaagcgaat taccggaaag
      241 tgccgggcat ctccggcgcg accatcatgg gcgatggtca tgttgcattg atcctggatg
      301 tctcggccat tgcgacaatg gccaggaatc atctgcacaa ggccgacgca gggccgttga
      361 ggagcgaatg atggaaatca ccaagcagca ggcaggcgcg ctggcggagg atgactcggt
      421 ggccagcgag ttcctgacct ttaccctggg ttgcgaggag tacgcgatcg acatcctgaa
      481 ggttcaggaa atccgcagct acgagacgcc gaccctgatc gccaatgcgc cacccttcat
      541 caagggggtg gtcaatctgc gcgggaccat cgttcccatt gtcgatctgc gcatcaagtt
      601 caatctcggc aagatcgaat acaccccatt tactgtcgtc gtcatcctca atatcgcccg
      661 tcgggtgatc ggtgtcgtcg tcgacagcgt ctccgacgtg ctgtcgttga cgtcgtcgca
      721 gatccggccg gcgccggatt tctccggctc gttcgacacc cgttacatcc ttggtctggc
      781 cagtgtcgag cagcggatgc tgatcgttac cgacatcgaa cggctgatga gcagcgcgga
      841 tatggaactg gtcgaagccg cagttgaatg aaccgcaaat aagggagcag ggggacgcta
      901 tgttgaacat gcgcaatttc aggattggca gccgcttgat cgcgacaacg gttggagcct
      961 tgtcgctgat gatcgctttc gtcgtcgtcg ccttgatcag cctggggatg atcggcgaga
     1021 aggtgcagac gatcgcggtc gataatgcgc agggcatggc gcttgccagc gccatgcgcc
     1081 gcgatgtgga cagccttggc ctgcatgcgc gcaatgccct gttgttcgaa gagctgctcc
     1141 gccagcgcga agcggcgcaa ggcgtaaccg atagcctcgc ccaattgcaa ctgctcgaga
     1201 agcagctcgg cgccaatctg ccgccggctg cccgcgatgt gatggaacgt ttgccggcct
     1261 tgcgcgaaga cgcggcagtt tcgctcaagc agttgtttgc gctgctcgcc gaggggaatc
     1321 ggccggtgct ggaaagtcat ttcgaaaatc acctggggcc gcgtctccgg gcctggcagg
     1381 aagccattga caccctgaat ctggcgcaaa aggcggaaac ctcgagtgcg ctcgaagaaa
     1441 ttgcgaccat ccgctcgacg gtgcagaacc tgctgttcgg cttgatcgtc ctggcggtgg
     1501 tggtgatgat tccttctggt atctgggtga ctcggggcat caccaacccg ctgtcggcag
     1561 ccgtcgacgt ggcggagtcg gtagccgccg gcaacttcga caaccgcatc gaaccgggcg
     1621 gagacgacga accggcgcac ctgttgcgga ccctggcgcg gatgcaggcc gacctcaagg
     1681 agcgcagcgc cagcgagcgg cggatcgccg cagaggcact gcgcatcaag gtggcactcg
     1741 atgtcggttc caactgcgtc atgttgctcg gtcccgacgg gcggatcatc tatggcaacg
     1801 acgccttgtt cggcatgatg cgcgaggcgg aggccgacat ccagcaacag ttgcccgagt
     1861 ttcgtgcggc ggcgatccgc agtgcggatt tctcagtccg cgagctgaac ccggcgctcg
     1921 ccggcgagca gctcggcgcg ctcgtcgaac cgcagcgcag cgtgatccgg atcgccaacc
     1981 ggaccttcgc cctggtgg
//
LOCUS       DAOSDX010000574         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19423_length_1995_cov_2.046392, whole genome shotgun sequence.
ACCESSION   DAOSDX010000574 DAOSDX010000000
VERSION     DAOSDX010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19423_length_1995_cov_2.046392"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PLN02_11700"
     CDS             complement(<1..1062)
                     /locus_tag="PLN02_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOI53036.1"
                     /translation="MSEENQLTDAEDLQERLAEVHTLLARHKLVEEIVHRQEGPRHDL
                     VEGIVHKQHLQELQHKLEPMHPADVAYIIEALPPDERLIVWDLVKAEHDGEILLEVSD
                     AVRESLIESMARNELVAAAETLDADELADLAPDLPQGVIDDVFRSLPAEEREHLRAAM
                     SYPEDAVGSIMDFDMVTVREDVTLEVVLRYLRRFDELPDHTDQLFVVDRDEHLKGVLP
                     LNILLVNEVEVEVGSLMHTEFVEFEPDDEAEDAAKAFERYDLVSAPVVDADGKLLGRV
                     TVNEVVDFIREEAEQEQLAHAGLAEEEDIFASVWDSVKNRWAWLAVNLVTAFVASRVI
                     GAFEDSIEKLVALAALMPII"
     gene            complement(1165..1860)
                     /gene="mtgA"
                     /locus_tag="PLN02_11705"
     CDS             complement(1165..1860)
                     /gene="mtgA"
                     /locus_tag="PLN02_11705"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOI53037.1"
                     /translation="MKRFGRWLRWGLLAPFALAFLWLLWLFLWVVYWGQFNPGTTRFM
                     EIRLAELREKNPDAVLRQQWVPYAQISVHLKRAIIAAEDAKFVDHEGFDWEGIQKAME
                     KNQKKGRFVAGGSTISQQLAKNLFLTPTKSYFRKAEEAVITLMLENLWSKRRIFEVYL
                     NVIEWGNGVFGAEAAARHYFAVAARQLGPAQAARLAGMVPNPRFYDRNRNAPGLGRKT
                     AIILARMPSAEVP"
     gene            complement(1857..>1995)
                     /gene="aroE"
                     /locus_tag="PLN02_11710"
     CDS             complement(1857..>1995)
                     /gene="aroE"
                     /locus_tag="PLN02_11710"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289630.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOI53038.1"
                     /translation="EAGAARLADGLGMLVGQAAEAFAVWRGVRPAGAPVLQALRAQLA
                     A"
BASE COUNT          326 a          661 c          649 g          359 t
ORIGIN      
        1 gatgatcggc atcagcgccg ccagcgccac cagcttctcg atcgaatcct cgaaggcgcc
       61 gatgacgcgc gaggcaacga aagcggtgac cagattgacc gccagccagg cccagcggtt
      121 cttcaccgaa tcccagaccg aggcgaagat gtcttcctcc tcggccagac cggcgtgcgc
      181 cagttgttcc tgctcggctt cctcgcggat gaagtcgacc acctcgttga cggtgacccg
      241 gccaagcagc ttgccgtcgg catcgaccac cggcgccgac accaggtcgt agcgctcgaa
      301 ggccttggcg gcgtcttcgg cctcgtcgtc cggttcgaac tcgacgaact cggtgtgcat
      361 caggctgccg acctcgacct cgacttcgtt gaccagcagg atgttgagcg gcagcacgcc
      421 cttcaggtgc tcgtcgcggt cgacgacgaa gagctggtcg gtatggtcgg gcagctcgtc
      481 gaaacggcgc aggtagcgca gcacgacttc gagcgtgacg tcttcgcgca cggtgaccat
      541 gtcgaagtcc atgatcgagc cgaccgcatc ctcgggatag gacattgccg cccgcaggtg
      601 ctcgcgctct tctgccggca ggctgcggaa aacgtcgtcg atgacgccct gcggcaggtc
      661 gggagcgagg tcggcgagtt cgtcggcatc cagggtttcg gcggcggcga ccagctcgtt
      721 gcgggccatc gactcgatca ggctctcgcg gaccgcatcc gagacttcga gcaggatttc
      781 gccgtcgtgc tcggccttga ccaggtccca gacgatcagg cgttcgtcgg gtggcagcgc
      841 ttcgatgatg taggcgacgt cggccgggtg catcggctcc agcttgtgct ggagttcctg
      901 caaatgctgc ttgtgcacga tgccttcgac cagatcgtgg cgcggccctt cctgccggtg
      961 cacgatctcc tcgaccagct tgtgccgggc gagcagggtg tgcacctcgg cgaggcgctc
     1021 ctgcaggtct tcggcatcgg ttagctggtt ttcttcgctc atggtgcagc gccgcaatag
     1081 gtgcgccatt ttagcaccgc tatggtgccg gccagccgcc caattgctgc ggatttccct
     1141 taggccggcg cttgctcggg cgactcaggg cacttcggcc gagggcatgc gagccaggat
     1201 gatcgccgtc ttgcgaccga gccccggcgc gttgcggttg cggtcgtaga aacgcggatt
     1261 gggcaccatg ccggccagcc gagccgcctg cgccgggccg agctggcggg cagcgacggc
     1321 gaagtaatgg cgggccgccg cttcggcgcc gaagacgccg ttaccccatt cgatcacgtt
     1381 gagatagacc tcgaagatgc ggcgcttgct ccacaggttt tccagcatca gcgtgatcac
     1441 cgcctcctcg gccttgcgga agtaggactt ggtcggcgtc aggaacaggt tcttggcgag
     1501 ttgctggctg atcgtcgacc cgccggcgac gaaacgcccc ttcttctggt tcttttccat
     1561 cgctttctgg ataccctccc agtcgaagcc ttcatgatcg acgaacttgg cgtcctcggc
     1621 ggcgatgatc gcccgcttca ggtgtaccga gatttgtgcg tagggcaccc actgctggcg
     1681 cagcacggcg tccggattct tctcgcgcag ctcggcgaga cgaatttcca tgaagcgggt
     1741 ggttcccggg ttgaactgcc cccaatagac cacccacagg aacagccaga gcagccacag
     1801 aaaagcgagc gcgaagggcg ccagcagccc ccagcgcagc cagcggccaa aacgtttcat
     1861 gccgcgagtt gcgcgcgcag cgcctgcagc accggcgcgc cggcgggacg gacgccgcgc
     1921 cagaccgcga aggcctcggc cgcctggccg accagcatgc cgagaccgtc agccaggcgc
     1981 gccgcgccgg cttcc
//
LOCUS       DAOSDX010000575         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19431_length_1994_cov_3.453842, whole genome shotgun sequence.
ACCESSION   DAOSDX010000575 DAOSDX010000000
VERSION     DAOSDX010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19431_length_1994_cov_3.453842"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="zapE"
                     /locus_tag="PLN02_11715"
     CDS             complement(<1..808)
                     /gene="zapE"
                     /locus_tag="PLN02_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOI53039.1"
                     /translation="MPHKKLKVSATGMLDAYDALLAERGYVADAAQRRAAQALQSLYG
                     NLLSFKVDRGSTIKRLLKPPKPPRGVYFWGGVGRGKSFLMDCFYASVPYRRKKRLHFH
                     SFMQQIHRDLDKYKGEPDPMLKLAAAIAQDVRLLCFDEFHVSDIADAMILGRLLEGLF
                     SHGVILVMTSNYPPDMLYPNGLHRESFLPAIALLKKHLDVFEVDAGVDYRLRALEQVE
                     IYHHPADDAAERKMLDYFRMVAGEEGEEGGQIEILDRQIATLRRGNGVIWF"
     gene            988..1488
                     /locus_tag="PLN02_11720"
     CDS             988..1488
                     /locus_tag="PLN02_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53040.1"
                     /translation="MELLVTLAVFYHLAALFASLTGVFLALCRLRGMATERRLIRPLI
                     LNGELHLWLSGLLIIALGVALKGMPYFDNPKLWTKVILITLWSLNSWLLRHYSRSGLD
                     QRRLAMFAISLGSLIYGSFLGVAKPLAYGVAPFWGLLAGYAACLLLTGLLLQRSRRWL
                     SSPAIA"
     gene            complement(1601..>1994)
                     /locus_tag="PLN02_11725"
     CDS             complement(1601..>1994)
                     /locus_tag="PLN02_11725"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370163.1"
                     /note="Catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOI53041.1"
                     /translation="GQSGHCDFNTIPGVVYTHPEIAWVGKTEQQLKAEGIAYKPGKVP
                     FLASGRALGHGDASGFVKVLADAKTDRILGVHIIGNNASELIAEAVVTMEFAGASEDL
                     GRICLAHPTLSEIVHEAALACDKRSLHF"
BASE COUNT          337 a          622 c          633 g          402 t
ORIGIN      
        1 cgaaccagat gacaccgttg ccgcgacgca gggtggcgat ctggcggtcg aggatttcga
       61 tctgcccacc ctcctcgccc tcctcgccgg cgaccatgcg gaaatagtcg agcatcttgc
      121 gctcggccgc gtcatcggcc ggatggtggt agatctcgac ctgctccagc gcgcgcagtc
      181 ggtagtcgac accggcatcg acctcgaaca cgtcgaggtg tttcttgagc agggcgatcg
      241 ccggcaggaa gctctcccgg tgcaggccgt tcgggtaaag catgtccggc gggtaattcg
      301 aggtcatgac caggatcacg ccatggctga acaagccctc gagcaggcgg ccgaggatca
      361 tcgcgtcggc gatgtcggag acgtggaatt cgtcgaaaca gagcagccgg acgtcctggg
      421 cgatcgccgc ggccagcttc agcatcggat ccggctcgcc cttgtacttg tcgagatcgc
      481 gatggatctg ctgcatgaag gagtggaaat gcaggcgctt cttgcgccgg tacggcaccg
      541 aagcgtaaaa gcagtccatc aggaaactct tgccgcgccc gacaccgccc cagaaataga
      601 cgccccgggg cggcttaggc ggcttcaaca ggcgcttgat cgtgctgccc cggtccacct
      661 tgaacgacag caggttgcca tagagcgact gcagggcctg cgccgccctc cgctgcgccg
      721 catcggcgac ataaccgcgc tcggcgagca gcgcatcgta ggcgtcaagc atgccggtgg
      781 cggagacttt cagtttcttg tggggcatcg tcggttgtcg ttgttctcgg gtccgctatt
      841 ttcgcaaata ttgacgaggt tctacagcgc aatgcgcttt accggaatga ccggctaacc
      901 gacggcgaac tctgcccccc ccctgtcatc agcgcgtcat ggcaatcgga ttgaatcatg
      961 gtttcgcatg ttcccggcgg atcgatcatg gaactcctcg tcaccctggc ggttttctat
     1021 cacctggccg ccctgttcgc gtcgctcacc ggagtcttcc tggcactgtg ccgtctgcgg
     1081 gggatggcga cggaaaggcg attgatccgg ccgttgatcc tgaacggcga gctccatctg
     1141 tggctttccg gcctgctgat catcgccctc ggtgtcgcgc tcaagggcat gccctatttc
     1201 gacaatccca agctgtggac gaaagtgatc ctgatcacgc tgtggagcct gaattcctgg
     1261 ctgcttcgcc actacagccg cagcggcctg gaccagcggc gcctagcgat gttcgccatc
     1321 tcgctcggtt cgctgattta cggcagtttc cttggcgtcg ccaaaccgct ggcttacggc
     1381 gtcgcaccgt tctggggctt gcttgccggc tatgccgcct gcctcctgct gacaggcctg
     1441 ctcctgcagc gatcgcgtcg ctggctgtcg tccccggcca tcgcctgacg gtagacccgc
     1501 gaaaactgca accgatcatt cactgtcgct gccggaacaa tgaaaaaaag cgggatgtgc
     1561 tattcgcaca tcccgccttt ggcagacgag gcaaaactgc ttagaagtgc agcgaccgct
     1621 tgtcgcaggc cagggcggct tcgtgcacga tttccgacag cgtcgggtgg gcgaggcaga
     1681 tgcggccgag gtcttcggag gcgccggcga attccatcgt gacgacggct tcggcgatca
     1741 gttcggaagc gttgttgccg atgatgtgca cgccgaggat gcggtcggtc ttggcgtcgg
     1801 ccaggacctt gacgaagccg ctggcgtcgc cgtggccgag cgcccggccg ctggccagga
     1861 acggcacctt gcccggcttg taggcgatgc cttcggcctt gagctgctgt tcggtcttgc
     1921 cgacccaggc gatttccggg tgggtgtaga cgacgcccgg aatggtgttg aagtcgcagt
     1981 gaccggactg gccg
//
LOCUS       DAOSDX010000576         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19437_length_1994_cov_2.037133, whole genome shotgun sequence.
ACCESSION   DAOSDX010000576 DAOSDX010000000
VERSION     DAOSDX010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19437_length_1994_cov_2.037133"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLN02_11730"
     CDS             <1..723
                     /locus_tag="PLN02_11730"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOI53042.1"
                     /translation="EGVNLSSLLFGNAAGLVETRRGKLDGQAPVLEKLAGTVQAGDVL
                     LEKTPFRLTDTFIPGHYGHVAVWVGSEAELRRLGIWEHPVVRPYQANIRAGRGVVEAL
                     RSGVKMNTLRHFLNIDDLAVLRPRQMADTVRAEAVLQTLRQVGKAYDFNFDVESTDRI
                     VCSELVYHAYGDVRWPTKRQLGRVTISPDNVAAEATGNGAFAIELLYHDGREQTAGRG
                     EFVRGLLKSDVVRMARGGDGAR"
     gene            complement(728..>1994)
                     /gene="lnt"
                     /locus_tag="PLN02_11735"
     CDS             complement(728..>1994)
                     /gene="lnt"
                     /locus_tag="PLN02_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOI53043.1"
                     /translation="FLAGVSWVYVSLSVFGGMPWWLAGPATLIFCAALALYPAAAGWA
                     FRRWQPAAFWRQVLWFAVLVAGTDWLRGWLFTGFPWLALGYSQAPPSPLAGFAPLLGV
                     YGLSLSVALIAASLARWRSGLLLLAAFAAVGFGLRQVTWTTPVGEPVSVALIQGNIPQ
                     DMKFRPEAFFRTLTLYRDLIAAHPAQLTLLPETALPTFVDRLPAEYLDELKTLAARRG
                     GDLIVGTLTGDVEGAYFNSAVSLGASPLQVYSKQHLVPFGEFIPPGFAWFMAQANIPM
                     SSFSAGPARQTPLAVADSAVAINICYEDVFGEEIIRALPAAGILANLSNTAWFGHSLA
                     QPQHLQIARLRAVETGRPMLRATNTGMTAIIAADGSLSGVLEPFTSGVLEGEVRAHQG
                     ATPYSRWGNLGALALIVLLALVARPRRRD"
BASE COUNT          334 a          653 c          735 g          272 t
ORIGIN      
        1 gaaggcgtca atctctccag cctgctgttc ggcaacgccg ccggcctggt cgagacgcgg
       61 cgcggcaagc tcgacggcca ggcgccggtg ctcgagaaac tggcgggaac ggtgcaggcc
      121 ggcgacgtgc tgctcgagaa gacgcctttc cgtctcaccg acaccttcat tcccggccat
      181 tacggccatg tcgcggtctg ggtcggcagc gaggcggaac tgcgccgcct cggcatctgg
      241 gagcatccgg tggtgcgtcc ctaccaggcc aacatccggg ccggccgcgg cgtcgtcgag
      301 gcgctgcgtt cgggggtcaa gatgaacacg ctgcggcatt tcctcaacat cgacgatctc
      361 gccgtcctgc gcccgcgcca gatggccgac acggtacgcg ccgaagccgt gctgcagacc
      421 ctgcgccagg tcggcaaggc ctacgacttc aatttcgacg tcgaatcgac cgatcgcatc
      481 gtctgctcgg aactggtcta tcacgcctac ggcgacgtgc gctggccgac caagcgtcaa
      541 ctcggccggg tcaccatcag tcccgacaac gtcgccgccg aagccaccgg caacggcgcc
      601 ttcgccatcg aactgcttta ccacgacggc agggagcaga ccgccgggcg cggcgagttc
      661 gtgcgcggcc tgttgaagtc cgatgtcgtc aggatggcgc gcggcggcga cggcgcccgc
      721 tgaaaattca atcgcgccgg cgcgggcggg cgacgagcgc gagcaggacg atgagggcga
      781 gggcgcccag gttgccccag cggctgtagg gcgtggcgcc ttgatgcgcc cgcacttcgc
      841 cttcgaggac gccgctggtg aagggctcca gcacgccgct caggctgccg tcggcggcga
      901 tgatcgcggt catcccggta ttggtcgcgc gcagcatcgg gcggccggtt tcgacggcgc
      961 gcaggcgggc gatctgcaga tgttgcggct gcgccagcga gtggccgaac caggcggtgt
     1021 tcgacaggtt ggcgagaatg ccggcggcgg gcagggcgcg gatgatctcc tcgccgaaca
     1081 cgtcttcgta gcagatgttg atcgccaccg cgctgtcggc gacggcgagc ggcgtctgtc
     1141 gcgccgggcc ggcgctgaac gacgacatcg gaatgttggc ctgggccatg aaccaggcga
     1201 agccgggcgg gatgaattcg ccgaaaggta ccaggtgttg cttgctgtag acctgcagcg
     1261 gcgaggcgcc gaggctgacg gcgctgttga agtaggcgcc ctcgacatcg ccggtcaggg
     1321 tgccgacgat caggtcgccg ccgcggcggg cggccagcgt cttcagttcg tcgaggtatt
     1381 ccgccggcag gcggtccacg aaggtcggca gcgcggtttc cgggagtagg gtcagttgcg
     1441 ccggatgggc ggcgatcaga tcgcggtaca gcgtcagcgt gcggaaaaag gcttccggcc
     1501 tgaacttcat gtcctgcggg atgttgccct ggatcagggc gacgctgacc ggctcgccga
     1561 ccggtgtcgt ccacgtcacc tggcgcaggc cgaagccgac ggcggcaaac gccgccagca
     1621 gcagaaggcc gctgcgccag cgggcgaggc tggcggcgat cagggcgacg ctcagcgaca
     1681 ggccataaac gccgagcagc ggcgcgaagc cggccagcgg gctgggcggt gcctggctgt
     1741 agccgagcgc cagccaggga aaaccggtaa acagccagcc gcgcagccag tcggttccgg
     1801 cgacgaggac ggcgaaccac agcacctggc gccagaaggc ggccggctgc cagcgccgga
     1861 aggcccagcc ggcggcggcc gggtagaggg ccagcgcggc gcagaagatg agcgtcgccg
     1921 ggccggccag ccaccacggc atgccgccga agaccgagag gctgacgtac acccaggaga
     1981 cgccggcgag gaag
//
LOCUS       DAOSDX010000577         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19445_length_1993_cov_3.127451, whole genome shotgun sequence.
ACCESSION   DAOSDX010000577 DAOSDX010000000
VERSION     DAOSDX010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19445_length_1993_cov_3.127451"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLN02_11740"
     CDS             <1..525
                     /locus_tag="PLN02_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HOI53044.1"
                     /translation="AQTRDHSRIRLLLDEGLITEAQAARHPDRNKIYSCLGGPSLPDI
                     DFSRKTPLEHSDVVLLCTDGLWGITSGEMMAVALKGANLLQVLPMLLNQAEAKAGSHG
                     DNLSAVAVCWEDNYVEALSSAISTQTMPVGEVTTKLDEFGRNPSYKSDLSDDEIEAAI
                     EEIRAAIDKFNPKI"
     gene            536..1252
                     /gene="rph"
                     /locus_tag="PLN02_11745"
     CDS             536..1252
                     /gene="rph"
                     /locus_tag="PLN02_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOI53045.1"
                     /translation="MRPSQRQPDQLRAVRITRNFTRHAEGSVLIEMGDTRVLCTASVE
                     ESLPPFLRGKGQGWVTAEYGMLPRSTHTRTAREAAKGKQTGRTQEIQRLIGRSLRAVT
                     DLKALGERQITLDCDVLQADGGTRCASITGAWIALYEACEKLVQAGKLAANPIKDHVA
                     AISVGIYNGSAVLDLDYPEDSNCDTDMNVIMTGNGGIVEVQGTAEGEPFSREQMNVLM
                     DLAQMGIRQLVAEQQKALAA"
     gene            1249..1845
                     /gene="rdgB"
                     /locus_tag="PLN02_11750"
     CDS             1249..1845
                     /gene="rdgB"
                     /locus_tag="PLN02_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOI53046.1"
                     /translation="MKKLVLASNNAKKMKELNALLAPLGFEVIPQGQLGIPEAEEPHC
                     TFVENALAKARHAAQHSGLPALADDSGLCVKALGGAPGVISARYAGEPKSDARNNEKL
                     LADLAAFPQMTERKAHFVCVLVLVRSADDPQPIIAEGEWHGEILAAPQGADGFGYDPL
                     FYVPTYACTAAELDPAAKNKLSHRGQAMQKLIERLPEL"
BASE COUNT          406 a          739 c          578 g          270 t
ORIGIN      
        1 gcccagacgc gcgaccactc gcgcatccgc ctgctgctcg acgaaggcct gatcaccgaa
       61 gcccaggccg cccgccaccc cgaccgcaac aagatctata gctgcctggg cgggccgagc
      121 ctgccggaca tcgacttctc gcgcaagacg ccgctcgaac acagcgatgt cgtgctgcta
      181 tgcaccgacg gcctgtgggg catcacctcc ggtgaaatga tggcggtcgc gctgaagggc
      241 gccaacctgc tgcaggtcct gccgatgctg ctcaaccagg ccgaagccaa ggccggcagc
      301 cacggcgaca acctgtcggc ggtcgccgtc tgctgggagg acaactacgt cgaagcgctg
      361 agcagcgcga tttcgaccca gaccatgccg gtcggcgaag tcaccaccaa gctcgacgaa
      421 ttcggtcgca accccagtta caagagcgat ctctccgacg acgagattga agcggcgatc
      481 gaggaaatcc gcgccgccat cgacaagttc aatccgaaga tataaaggaa tccccatgcg
      541 ccccagccaa cgccagccgg accagctccg tgccgtccgc atcacccgca atttcacccg
      601 tcacgccgaa ggttcggtgc tgatcgaaat gggcgacacg cgcgtgctgt gcaccgcctc
      661 ggtcgaggaa agcctgccgc ccttcctgcg cggcaagggc cagggctggg tcaccgccga
      721 gtacggcatg ctgccgcgct cgacgcacac gcgcacggcg cgcgaggcgg ccaagggcaa
      781 gcagactggc cgcacccagg aaatccagcg cctgatcggc cgcagcctgc gcgccgtgac
      841 cgacctcaag gccctcggcg aacgccagat caccctcgac tgcgacgtcc tgcaagccga
      901 cggcggcacc cgctgcgcct cgatcaccgg cgcctggatc gccctctatg aagcctgcga
      961 aaagctggta caggccggca agctagccgc caacccgatc aaggaccacg tcgccgcgat
     1021 ctcggtcggc atctataacg gcagcgcggt gctcgacctc gactacccgg aagactccaa
     1081 ctgcgacacc gacatgaacg tcatcatgac cggcaacggc ggtatcgtcg aagtccaggg
     1141 cacggccgaa ggcgagccgt tcagccgcga gcagatgaac gtgctgatgg acctcgcgca
     1201 gatgggcatc cgccaactgg tcgccgaaca gcaaaaagcg ctcgccgcat gaaaaagctg
     1261 gtcctcgcct cgaacaacgc caagaagatg aaggagctga acgcgctgct cgcgccgctc
     1321 ggcttcgaag tcatcccgca aggccagctt ggcatcccgg aagccgagga accgcactgc
     1381 accttcgtcg agaacgcgct cgccaaggcc cgccacgccg cccagcacag cggcctgccg
     1441 gcgctcgccg acgactccgg cctgtgcgtc aaggccctcg gcggcgcccc cggcgtgatc
     1501 tccgcccgct atgccggcga accgaaatcc gacgcgcgca acaacgaaaa gctgctcgcc
     1561 gacctcgccg ccttcccgca gatgaccgag cgcaaggcgc acttcgtctg cgtcctcgtc
     1621 ctcgtgcgta gcgccgacga cccgcagccg atcatcgccg aaggcgaatg gcacggcgaa
     1681 atcctcgccg ccccgcaagg cgccgacggt ttcggctacg acccactgtt ctacgtcccc
     1741 acctacgcgt gcaccgccgc cgaactcgac ccggcggcca agaacaaact ctcacatcgc
     1801 ggccaggcga tgcagaaact gatcgagcgg ctgccggaac tctgacgcct cacctcgccc
     1861 gccgtttccg gtatgctgct cgcccagcag acttgattca tttgtcatcc ggaattcagc
     1921 catgagcgaa cagcccctcc gcttcactga accgccggcc ggctacaccg actggctggc
     1981 cgacctgaaa acc
//
LOCUS       DAOSDX010000578         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19447_length_1993_cov_2.940660, whole genome shotgun sequence.
ACCESSION   DAOSDX010000578 DAOSDX010000000
VERSION     DAOSDX010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19447_length_1993_cov_2.940660"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..>1993)
                     /locus_tag="PLN02_11755"
     CDS             complement(19..>1993)
                     /locus_tag="PLN02_11755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI53047.1"
                     /translation="LKAGRHEAGSHAARVHSGMAAVSDTVFDAAVRRAGVVRVKNVGQ
                     LFYAAKALASKFRPQGDRLAIITNGGGPGAMAADRAGDLGIPLAELSAETMGNLNKVL
                     PANWSHSNPIDIGGDATPERYRDALLSITQDPNVDSTLVMLSPQAMTDPLGVAQAIVS
                     ISDKLNRRIICCWMGEAQVAEARRLLEDSGIPAFRMPETAIELFHHISKYYRNQKLLL
                     QTPEPTRQHNRPEAEGAKMLIEALLAERRKVLSEMESKAILRAFKVPVAQTMVARTAT
                     EALLLAEQIGFPVAMKVDSPDLPHKSDAGGVRLNIQNAPAVRNAYHDIIETVQKRNPN
                     AKINGVSIEPFLSRPHGREIMIGVLRDPIFGPVITFGAGGFDNEIFSDRSVALPPLNK
                     FLAKDLIDSTRTAKILDQFHNMPPVDREALKEVLLCISEMVCELPWIIEMDLNPLIVD
                     EHGAIAADARIVIDHASSSSGDRYAHMAIYPYPVHLVQEWTMNDGQVVTVRPIRPEDA
                     DLEQEFVKSMSDESRYYRFMDTLRELTQTMLVRFTQIDYDREMALVATIADDETGKEK
                     QIGVARYVLNPDGESVEFALAVGDDWQKHGVGRKLMTALIDCARTKGYRAVVGDVLST
                     NTKMFRLMTSLGFTIHPHPEDTAVKRVVKPLTG"
BASE COUNT          308 a          590 c          709 g          386 t
ORIGIN      
        1 ttgtgggcgc gacgccgatc agccggtcag cggtttgacc acgcgcttga ccgcggtgtc
       61 ttccgggtgc gggtggatgg tgaaaccgag gctggtcatc agacggaaca tcttggtgtt
      121 ggtcgacagt acgtcgccga cgacggcgcg gtaacccttg gtgcgggcac agtcgatcag
      181 cgccgtcatc agtttgcggc cgacgccgtg cttctgccag tcgtcgccga cggcgagcgc
      241 gaactcgacc gactcgccgt ccgggttgag cacgtagcgg gcgacgccga tctgcttttc
      301 cttgccggtt tcgtcatcgg cgatggtcgc cacgagtgcc atttcacggt cgtagtcgat
      361 ctgcgtgaag cgcaccagca tggtctgggt cagttcgcgc agggtgtcca tgaagcggta
      421 gtagcgcgat tcgtcggaca ttgatttgac gaattcctgc tccaggtcgg cgtcttccgg
      481 gcggatcgga cggacggtga cgacctggcc gtcgttcatc gtccattcct ggaccaggtg
      541 caccggatac gggtagatcg ccatgtgcgc gtagcggtca ccggaactgc tgctggcgtg
      601 gtcgatgacg atgcgcgcat cggcggcgat ggcgccatgc tcgtcgacga tcagcgggtt
      661 gaggtccatc tcgatgatcc acggcagttc gcaaaccatt tccgagatgc agagcaggac
      721 ttccttgagc gcttcgcggt cgaccggcgg catgttgtgg aactggtcga ggatcttggc
      781 ggtacgcgtc gagtcgatca ggtccttggc caggaacttg ttcagcggcg gcagggcgac
      841 cgaacggtcg ctgaagattt cgttgtcgaa gccgccagca ccgaaagtga tgaccggccc
      901 gaagatcggg tcgcgcagca cgccgatcat gatttcgcgg ccgtgcggac gggacaggaa
      961 gggctcgata gagacgccat tgatcttggc gttcggattg cgcttctgca ccgtttcgat
     1021 gatgtcgtgg taggcgttgc gtaccgccgg cgcattctgg atgttgaggc ggacgccgcc
     1081 ggcgtcggac ttgtgcggca ggtccggcga atcgaccttc atcgccaccg ggaagccgat
     1141 ctgctcggcg agcagcaggg cttcggtggc ggtgcgggcg accatggtct gggcgaccgg
     1201 caccttgaag gcgcgcagga tggccttcga ttccatttcc gacagcacct tacgccgctc
     1261 ggcgagcagc gcctcgatca gcatcttggc gccttcagcc tcggggcggt tgtgctggcg
     1321 cgtcggttcc ggcgtctgca gcagcagctt ctggttgcgg tagtacttgg agatgtggtg
     1381 gaacaactcg atcgccgttt ccggcatgcg gaaggccgga atgccggagt cttcgagcaa
     1441 tctgcgcgct tcggccacct gagcctcgcc catccagcag cagatgatgc ggcggttgag
     1501 cttgtcggag atcgagacga tggcctgggc gaccccgagc gggtcggtca tcgcctgcgg
     1561 cgagagcatg accagcgtgc tgtcaacgtt cgggtcctgg gtgatcgaca acagcgcgtc
     1621 gcggtagcgc tccggagttg cgtcgccgcc gatgtcgatc ggattgctgt gcgaccagtt
     1681 ggccggcagc accttgttca ggttgcccat cgtctcggcc gacagttcgg cgagcggaat
     1741 gccgaggtcg ccggcgcggt cggcggccat cgcgcccggg ccaccgccgt tggtgatgat
     1801 cgccaggcgg tcgccttgcg ggcggaactt ggaagccagc gccttggcgg cgtagaacag
     1861 ctggccgacg ttcttgacgc ggacgacgcc ggcgcggcgc acggcggcgt cgaagacggt
     1921 gtcggaaaca gccgccatgc ccgagtggac gcgcgcggcg tgcgagccgg cctcgtggcg
     1981 accggccttc agc
//
LOCUS       DAOSDX010000579         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19456_length_1992_cov_2.760454, whole genome shotgun sequence.
ACCESSION   DAOSDX010000579 DAOSDX010000000
VERSION     DAOSDX010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19456_length_1992_cov_2.760454"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /gene="lolB"
                     /locus_tag="PLN02_11760"
     CDS             <1..602
                     /gene="lolB"
                     /locus_tag="PLN02_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HOI53048.1"
                     /translation="AGRGDQEVPALIRRLIGCCAALLVAACAQLPAGPLPQRGELENF
                     AVAGRFALLTRQADGSSESTGGRLDWTHSRDGDRLLLANPLGIGVAELYSGGGQARLI
                     TSDGQTREAADAEQLLAEVTGQALPIGRLAGWLLGRAGPGGRLERDAFDRPLRLDEAG
                     WRVDYAYADDDPAAPPMRLTASNAVLELRLRIETWKALP"
     gene            599..1450
                     /gene="ispE"
                     /locus_tag="PLN02_11765"
     CDS             599..1450
                     /gene="ispE"
                     /locus_tag="PLN02_11765"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOI53049.1"
                     /translation="MNDWNWLSRWPAPAKLNLFLHVVGRRADGYHLLQTVFRFIDRCD
                     WLRFSPRADGRIELATPTPGVAPDDDLTVRAARLLQAHTGCRQGATIHLEKTLPMGGG
                     LGGGSSDAASVLLALNHLWQTGVPRGELENLGLALGADVPVFVHGRNCFAEGVGETFT
                     DLDLPPAAYLILQPPVLVPTAAIFGSPDLRRDTPAMRPADWRPGIGGNDLEAVACAAF
                     PAVASHLVWLRRHSPDAMMTGSGACVFAAFGSEDAAATACAGMPEGMIGWVANGLAQH
                     PLAEIAR"
     gene            1523..1599
                     /locus_tag="PLN02_11770"
     tRNA            1523..1599
                     /locus_tag="PLN02_11770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1557..1559,aa:Gln,seq:ttg)
     gene            1686..>1992
                     /gene="prs"
                     /locus_tag="PLN02_11775"
     CDS             1686..>1992
                     /gene="prs"
                     /locus_tag="PLN02_11775"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOI53050.1"
                     /translation="MAYNSLMVFTGNANPKLASDVAKHLNVDLGRANVGRFSDGEVSV
                     ELQEHVRGRDIFVIQSTCAPTNDNLMELLVMVDSLKRASAGRITAAIPYFGYARQDRR
                     "
BASE COUNT          303 a          707 c          635 g          347 t
ORIGIN      
        1 acgctgggcg cggtgatcaa gaagtacctg ccttgatccg tcgtctgatc ggctgctgcg
       61 ccgcgctgct cgtcgctgcc tgtgcccagt taccggcagg gccgttgccg cagcgcgggg
      121 aactggagaa tttcgccgtc gccggccgct ttgcgctgct cacccgtcag gccgacggca
      181 gcagcgaaag tacgggcggc cgcctcgact ggacccacag ccgggacggc gaccgtctgc
      241 tgctcgccaa cccgcttggc atcggcgtcg ccgaactgta cagcggcggc ggccaggcgc
      301 ggctgatcac cagcgacggc cagacccgcg aagctgccga cgccgaacaa ctgctcgccg
      361 aagtcaccgg ccaggcgctg ccgatcggcc gcctcgccgg ctggctgctc ggccgcgcag
      421 gccccggcgg tcgtcttgag cgcgacgcct tcgaccgtcc gctgcgcctc gacgaagccg
      481 gctggcgcgt cgactatgcc tacgccgacg acgatccggc ggcgccgccg atgcgcctga
      541 cggcgagcaa cgcggtgctc gagctgcggc tgcgcatcga aacctggaaa gccctgccat
      601 gaacgactgg aactggctca gccgctggcc ggcgccggcc aagctcaacc tcttcctgca
      661 tgtcgtcggc cgccgtgccg acggttacca cctgctgcag acggtgttcc gcttcatcga
      721 tcgctgcgac tggctgcgct tctcgccgcg cgccgatggc cgcatcgagc tggcgacacc
      781 gacgccgggc gtcgctccgg acgacgatct gacggtgcgc gccgcccgct tgctgcaagc
      841 gcataccggc tgccggcagg gggcgacgat tcatctcgag aagacgctgc cgatgggcgg
      901 cggtctcggc ggcggttcgt cggatgcggc cagcgtcctg cttgccctca accacctgtg
      961 gcaaaccggt gtgccgcgcg gcgaactgga aaatctgggt ctggcgctgg gcgccgacgt
     1021 gccggtcttc gtccacggcc gcaactgttt cgccgaaggc gtcggcgaga ccttcaccga
     1081 tctcgacttg ccgccagccg cctatctcat tctgcaaccg ccggtgctgg taccgacggc
     1141 ggcgatcttc ggttcgccgg acttgcgtcg cgacacgccg gcaatgcgcc ccgccgactg
     1201 gcgaccgggc atcggcggca acgatctgga agccgtcgcc tgcgccgctt ttcccgccgt
     1261 cgccagccac ctcgtgtggc tgcgccggca ttcgccggac gccatgatga ccggctccgg
     1321 cgcctgcgtc ttcgccgcct tcggcagcga ggatgcggct gcgacagcct gcgccggaat
     1381 gccggaaggc atgatcggct gggtcgccaa cgggctggcg caacatccct tggcagaaat
     1441 tgcacgatag ggctggattt ccgtaaaacc ctcctgtatc attcgcgcct cgttcgaacg
     1501 acaccccgtc cgaacgaacc gcaggggagt cgccaagttg gtcaaggcac cggattttga
     1561 ttccggcatt cgaaggttcg aatccttcct cctctgccag tttcccttcg ggcaggtctt
     1621 aggcctgccc gattttcatt gtatcgggcc ttcgcggcgc gcgcggagcc cggaggaatg
     1681 cggaaatggc ctataacagc ctgatggtct tcaccggcaa tgccaacccc aagctggcat
     1741 ccgacgtcgc caagcatctc aacgtcgatc tcggtcgtgc caacgtcggc cgtttctcgg
     1801 acggtgaagt cagcgtcgaa ctgcaggaac acgtccgcgg tcgcgacatc ttcgtgatcc
     1861 agtcgacctg cgccccgacc aacgacaacc tgatggaatt gctggtcatg gtcgattcgc
     1921 tgaagcgcgc ttcggctggt cgcatcaccg ccgccattcc ctatttcggc tacgcccgcc
     1981 aggatcgccg ct
//
LOCUS       DAOSDX010000580         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19469_length_1991_cov_3.783058, whole genome shotgun sequence.
ACCESSION   DAOSDX010000580 DAOSDX010000000
VERSION     DAOSDX010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19469_length_1991_cov_3.783058"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLN02_11780"
     CDS             complement(<1..765)
                     /locus_tag="PLN02_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HOI53051.1"
                     /translation="MGAPEIVATVIFAIAVLHTFSTKFFAHLAHINPRHAGLWHLLAE
                     VEVVFGFWAFVLIASLFLLSGQNAAVHYLEGRNFTEPMFVFVIMVMAASRPILVAVAA
                     LIQQAAAMIPMNRALSTYFLCMAVVPMLGSFITEPAAMTLAALMLRDRFFQQGLSTRL
                     KYATIGVLFVNVSIGGTLTHFAAPPVLMVAAKWQWDTLFMISHFGWKSAVAVFANAAV
                     LTWMFRQELSGKGALGGKSGSVVPVAVTSVHVIFLVG"
     gene            complement(952..1227)
                     /locus_tag="PLN02_11785"
     CDS             complement(952..1227)
                     /locus_tag="PLN02_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOI53052.1"
                     /translation="MQIGVAYSEPGQQIWLNIEVPDSATIREAIERSGILKQFPQIDL
                     DVQKVGVFGKLSKLDSALRPGDRVEIYRAIICDPATVPRKDADDDEE"
     gene            complement(1239..>1405)
                     /locus_tag="PLN02_11790"
     CDS             complement(1239..>1405)
                     /locus_tag="PLN02_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314920.1"
                     /note="in Excherichia coli RsxABCDEG reduces SoxR which
                     turns off induction of SoxS transcription factor in the
                     absence of oxidizing agents; similar to the rnfABCDGE
                     operon in Rhodobacter capsulatus involved in transferring
                     electrons to nitrogenase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOI53053.1"
                     /translation="FAFLEVQVPGYGGALLMILPPGGFAVLGFMLAGKRIIDQKLAAR
                     AQAAATPATA"
     assembly_gap    1406..1415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1416..1916)
                     /locus_tag="PLN02_11795"
     CDS             complement(<1416..1916)
                     /locus_tag="PLN02_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOI53054.1"
                     /translation="MSDNNYGTIVKDGLWEQNVVFAQMLALCPTMAVTTSGTNGLGMG
                     LATTAVLVVSNILVSIIRKTVSDQVRIPVFVVLIATLVTIVDLAMNAWVHGLYKVLGL
                     FIALIVVNCAILGRAEAYAVKNGVLASAVDGLAMGIGFTGALTLIGLVREFLGSGTLF
                     AQASQLL"
BASE COUNT          411 a          612 c          647 g          311 t
ORIGIN      
        1 gccgacgagg aagataacgt gcaccgaagt caccgccacc ggcaccactg agccgctttt
       61 gccaccgagc gcgcccttgc cggaaagttc ctggcggaac atccaggtca gcacagcggc
      121 gttggcgaag acggcgacgg cggatttcca gccgaaatgg gaaatcatga acaaggtgtc
      181 ccattgccac ttggcggcga ccatcagcac cggcggcgcc gcgaagtggg tcagcgtgcc
      241 gccgatcgag acgttgacga agaggacgcc gatggtcgcg tacttgaggc gggtcgacag
      301 cccctgctgg aagaaacggt cacgcagcat caaggccgcc agcgtcattg ccgccggttc
      361 ggtgatgaag gaaccgagca tcggcaccac ggccatgcac aggaaatagg tggatagcgc
      421 ccggttcatc gggatcatcg ccgccgcctg ctggatcagc gccgcgacgg cgaccaggat
      481 cggccggctg gcggccatca ccatgatcac gaagacgaac atcggctcgg tgaaattgcg
      541 cccttccagg taatgcaccg cggcattctg cccggacaac aggaacagcg aggcgatcag
      601 gacgaaagcc cagaaaccga acaccacctc gacctcggcg agcaggtgcc agagcccggc
      661 atggcgggga ttgatgtggg cgagatgagc gaagaatttg gtggagaagg tgtgcagcac
      721 ggcgatcgcg aagatcaccg tggcgacgat ttccggggca ccaatcccgc cgccggcagc
      781 agcgggtggc gcgatttcgg aactggcgaa ggccagggca ggcagagaca acagggacag
      841 cgcgagaaaa cgtagcatgg tggatggcga ccaggaaata ttaggaaaac cggatgtatt
      901 gtcgactgcc cgactcaagt tttcaacagt tcaaaacgca tttacggcaa ttcattcctc
      961 atcgtcgtcg gcgtccttgc gcggcacggt cgccggatcg cagatgatgg cccggtagat
     1021 ctcgacgcgg tcgccggggc gcaaggcgct gtcgagcttg gacaacttgc cgaaaacgcc
     1081 gaccttctgc acgtcgagat cgatctgcgg aaactgcttg agaatgccgg agcgctcgat
     1141 cgcctcgcgg atcgtcgcgc tgtcgggcac ttcgatgttg agccagattt gctggccggg
     1201 ttcggaatag gcaacgccga tctgcatggc gattctcctt aagcggtggc cggcgtggcg
     1261 gccgcctggg cgcgcgcggc cagtttctga tcgatgatgc gcttgccggc cagcatgaag
     1321 ccgagcacgg cgaagccccc gggcggcagg atcatcagca gcgcgccgcc gtaaccgggc
     1381 acctgcactt cgaggaaggc aaaggnnnnn nnnnngagca gttgcgaggc ctgcgcgaac
     1441 agcgtgccgc tgccgagaaa ctcgcgcacc aggccgatca gcgtcagcgc gccggtgaaa
     1501 ccgatgccca tcgccaagcc atcgacggcg ctcgccagca cgccgttctt caccgcgtag
     1561 gcttcggccc ggccgaggat ggcgcaattg acgacgatca gcgcgatgaa caggccgagt
     1621 accttgtaca gcccatgcac ccaggcattc atcgccaggt cgacgatggt caccaaggta
     1681 gcgatgagga cgacgaagac cgggatgcgc acctggtcgc tgaccgtctt gcggatgatc
     1741 gagaccagga tgttcgacac caccagcacc gccgtcgtcg ccagccccat gcccaggccg
     1801 ttggtgccgc tggtggtcac cgccatggtc ggacacagcg ccagcatctg ggcgaagacg
     1861 acgttctgct cccagaggcc gtccttgacg atggttccgt agttgttgtc gctcatgatc
     1921 cgctcccggc cgtgatgtcc tgcttgcggg cggcgtagaa ctcgagcccg cccttgaccg
     1981 ccttgaccac c
//
LOCUS       DAOSDX010000581         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19517_length_1987_cov_4.151139, whole genome shotgun sequence.
ACCESSION   DAOSDX010000581 DAOSDX010000000
VERSION     DAOSDX010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19517_length_1987_cov_4.151139"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1325)
                     /locus_tag="PLN02_11800"
     CDS             complement(54..1325)
                     /locus_tag="PLN02_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOI53055.1"
                     /translation="MLALEISPHLPADCRPRSQQPTAEDLCIQTPSVTAEDSNETVME
                     IFSKYRDLTSLPVIEDGIPIGLINRGIFLSQMSKPFRKEVYSRKSSIAFMDKEPLVVE
                     ADMDIESLTFKAVEYGEKALADGFIITRGGRFAGVGHGLQLMRVVADMQAARNRQIMQ
                     SIEYASVIQRSMLRTSRDTMLHTLEDADLVWEPRDLVGGDFYYFVARSDGWFGAVADC
                     TGHGVPGAFMTLIASSCLAQAIDQHGPADPALLLGSVNCGIKQMLGQVGGQDETPESD
                     DGMDAACFWYDIATRRLIFAGAKTSLFVLHPGGDGVEMVDGDRKCVGYVDSELDFTWQ
                     KRAVQMQAGSLVFVTTDGLVDQVGGPKSIAFGKRRIRDLILANRDRPSSEINRAVQQA
                     ISEWQGPNHRRDDVTFFCFRVQEAQRTNQAS"
     gene            complement(1530..>1987)
                     /locus_tag="PLN02_11805"
     CDS             complement(1530..>1987)
                     /locus_tag="PLN02_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOI53056.1"
                     /translation="PLHDIGKVGIPDHILLKPGKLDDAEFEIMKTHTTLGLEAIRRAE
                     QHLEGEDGFLRIAGEIAHAHQEKWDGSGYPLGLAGEAIPLSARLMAVADVYDALISKR
                     VYKPAFSHEQAVAIILDGSGKHFDPELIAAFREIEDEFRAIARQFQDHE"
BASE COUNT          363 a          592 c          640 g          392 t
ORIGIN      
        1 gtagaaaatc acccgttgct ggcaggcaag ctcaaaaaaa gagccgaagt ccttcatgag
       61 gcttgatttg tccgttgcgc ttcctgaacg cggaagcaga aaaaggtgac gtcatcgcga
      121 cgatggttcg gcccctgcca ttcgctgatc gcctgctgta cggcgcggtt gatctcgctg
      181 ctcggtcggt cgcggttggc caggatgagg tcgcggatac gacgcttgcc gaaggcaatg
      241 gacttggggc cgccgacttg gtcgaccaga ccgtcggtgg tgacaaacac caggctgcca
      301 gcttgcatct gcaccgcccg cttctgccag gtgaaatcca gctcgctatc gacgtagccg
      361 acgcacttgc ggtcgccatc caccatttcc accccatcgc caccggggtg cagcacgaag
      421 aggctggtct tggcgccggc gaatatcagc cggcgggtgg cgatgtcgta ccagaaacag
      481 gcggcatcca tgccgtcgtc cgattccggg gtttcatcct gcccgccgac ctgtccgagc
      541 atctgcttga tgccgcagtt gacgctgccc agtaagagcg ccgggtcagc agggccatgc
      601 tggtcaattg cctgggccag acaactggaa gcgatcagcg tcatgaaggc gccgggcacg
      661 ccgtgaccgg tacaatccgc gacggcaccg aaccagccat cgctgcgggc aacgaagtag
      721 tagaaatcgc cgccgacgag atcgcgcggc tcccaaacca gatcggcgtc ctccagtgtg
      781 tgcagcatgg tgtcgcgcga agtacgcaac atggaacgct gaatcacgct ggcgtattcg
      841 atgctctgca tgatctggcg gttgcgggct gcctgcatgt cggccaccac ccgcatcagt
      901 tgcaaaccgt ggccgacgcc ggcgaagcgg ccgccgcggg tgatgatgaa accatcggcc
      961 agcgccttct cgccgtattc caccgccttg aaggtcaacg actcgatgtc catgtccgcc
     1021 tcgaccacca gcggctcctt gtccataaag gcaatggaac tcttgcggct gtatacctcc
     1081 ttgcggaacg gcttgctcat ctgcgacagg aagatgccgc ggttgatgag gccgatgggg
     1141 ataccatcct cgatcactgg cagactcgtc aggtcgcggt acttggagaa tatctccatg
     1201 accgtttcat tgctgtcttc agccgtgacc gagggcgtct ggatgcagag atcttcggcg
     1261 gtcggctgct gagagcgcgg acgacaatcg gcggggagat ggggggatat ttccaaggca
     1321 agcattccgg aaaatggggt tttgcgttat ctgtttgcgg cgaactcgtc ggcgacagtc
     1381 taggcagttc gtgtgtccaa tcggtgacaa gacaggcgca agatgtggct tttcttccgg
     1441 aatgcgccgg ctgcaggctg ccccaaggcg aacccgcaga gcgaaaggag gcggcacgct
     1501 tcggcgctgt cgctacgccg gagcctggcc tattcgtgat cctggaactg ccgggcgatg
     1561 gcgcggaatt cgtcctcgat ttcccggaag gcggcgatca gttcggggtc gaaatgcttg
     1621 ccactgccgt cgaggatgat ggcgaccgcc tgttcgtggc tgaaggccgg cttgtagacg
     1681 cgcttgctga tcagcgcatc atagacgtcg gccaccgcca tcagccgcgc cgacagcgga
     1741 atggcttcgc cagcgaggcc tagcgggtag ccgctgccgt cccatttctc ctggtgtgca
     1801 tgggcgattt cgccggcgat gcgcaggaag ccgtcctcgc cttcgaggtg ctgctcggcg
     1861 cggcggatcg cttcgaggcc gagcgtggtg tgcgtcttca tgatttcgaa ttcggcgtcg
     1921 tcgagcttgc ccggcttgag caggatgtgg tcgggaatgc cgaccttgcc gatgtcgtgc
     1981 agcggcg
//
LOCUS       DAOSDX010000582         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19518_length_1987_cov_3.292961, whole genome shotgun sequence.
ACCESSION   DAOSDX010000582 DAOSDX010000000
VERSION     DAOSDX010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19518_length_1987_cov_3.292961"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLN02_11810"
     CDS             complement(<1..1239)
                     /locus_tag="PLN02_11810"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOI53057.1"
                     /translation="MEFSIKSGSPEKQRSNCVVVGVFEHRRLTLSAELLDNAASGMIS
                     DILRRGDMNGKAGSTLLLHNVPGTLCERILLVGLGKEKDFREKEYCAALRLAVKTVGE
                     TGAQDATLFLTEIAVRKRDAAWRIRQAVVCSFAAGYRFDHYRSKDDEPRRLRRLTLIV
                     ERRSELAAAEEALRQGQAIGEGCQWARDLGNQPPNVCNPAYLAEQAQALAEELGLGCE
                     ILDRPAMAALGMNALLAVARGAAAEPRLIVLQYKGAKAADKPVVLVGKGVTFDSGGIS
                     LKPGADMDEMKYDMGGAAAVLGTIKAVARMALPLNLTVIVPAAENMPDGNATRPGDIV
                     STLSGQTVEILNTDAEGRLLLCDALTYAERFNPDTVIDVATLTGACVVALGHVASGLF
                     ASKDGLARDLIEAGEETGDRA"
     gene            1312..>1987
                     /gene="lptF"
                     /locus_tag="PLN02_11815"
     CDS             1312..>1987
                     /gene="lptF"
                     /locus_tag="PLN02_11815"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOI53058.1"
                     /translation="MIFERAARREFAQAAAGIVVVLLAIVTSIQLIRLLKDAAGGELA
                     PEGVLALLGFALVKFLPMLLSLTLFVAVLMSLSRMYRDSEMVVWLNSGMSLMAWIRPV
                     LLFALPIMITVGGLSLVLAPWANQAQSEFQAGLSARSELSSVSPGTFKEVRGGRHVYF
                     IERLDDVAGKIGNVFVASLQEGKLGVVMSDSGAQEVHENGSRFLVLQRGRRYEVEPGS
                     AAFRIMD"
BASE COUNT          286 a          612 c          682 g          407 t
ORIGIN      
        1 ggcgcggtcg ccggtttcct cgccggcctc gatcaggtct cgggcgaggc cgtccttgct
       61 ggcgaacagg ccgctggcga catggccgag ggcgacgacg caggcgccgg tcagggtggc
      121 gacgtcgatc accgtgtccg gattgaagcg ctcggcgtag gtcagcgcat cacagaggag
      181 caagcggcct tcggcgtcgg tgttgaggat ctcgacggtc tgcccggaca gcgtgctgac
      241 gatgtcgccc gggcgcgttg cgttgccgtc gggcatgttt tccgccgccg ggacgatcac
      301 cgtcaggttg agcggcaggg ccatccgggc gaccgccttg atcgtgccaa ggacggccgc
      361 ggcgccgccc atgtcgtact tcatctcgtc catgtcggcg cccggcttca gcgagatgcc
      421 gccggaatcg aaggtgacgc ccttgccgac caggacgacc ggtttgtccg ccgccttggc
      481 gcccttgtac tgcaggacga tcagccgggg ttctgcagcg gcgccgcgcg ccaccgcgag
      541 cagggcattc atgccgagcg cggccatcgc cgggcgatcg aggatttcgc agccgaggcc
      601 gagttcttcg gccagcgcct gcgcctgttc ggccaggtag gcgggattgc agacattggg
      661 cggctggttg ccgaggtcgc gcgcccactg gcagccttca ccgattgcct ggccctggcg
      721 caaggcctct tcggcggcgg ccagttcgct gcgccgttcg acgatcaggg tcagccggcg
      781 caggcggcgc ggctcgtcgt ccttgctgcg gtagtggtcg aaacggtagc cggcggcgaa
      841 cgagcagacc acggcctggc ggatgcgcca ggcagcgtcg cgcttgcgga cggcgatttc
      901 ggtgaggaaa agagtggcgt cctgggcgcc ggtttcgccg acggtcttca ccgccaggcg
      961 cagggcggcg cagtattcct tttcacggaa gtccttttcc ttgcccagac cgaccagcag
     1021 gatgcgttcg cacagggtgc cggggacgtt gtgcagcagc agcgtgctgc cagccttgcc
     1081 gttcatgtcg ccacggcgga ggatgtcgct gatcatgccg ctggcggcgt tgtcgagcag
     1141 ttcggccgac agcgtcaacc gccggtgctc gaaaacgccg acgacgacgc agttgcttcg
     1201 ctgcttttcc gggcttccgc tttttatgct aaattccacg cgctgctcct gtcccaggga
     1261 aatatcggca tcccgtggat tatccacgca atttttcggt ttcgtcaaag catgatattc
     1321 gaacgcgccg cgcggcgcga attcgcccag gcagcggccg gcatcgtcgt cgtattgctg
     1381 gcaatcgtca cctcgataca gttgatccgc ctgctcaagg atgcggccgg cggcgagctt
     1441 gcgccggaag gcgtgctggc cttgctcggc ttcgcgctgg tcaagttcct gccgatgctg
     1501 ctcagcctga ccctgttcgt tgccgttttg atgtcgctgt cacggatgta ccgcgattcg
     1561 gagatggtcg tctggttgaa cagtggcatg tcgttgatgg cctggatcag gccggtgttg
     1621 ctttttgcgt tgccgatcat gattaccgtc ggcgggcttt cgctggtcct ggcgccgtgg
     1681 gccaaccagg cgcagagcga attccaggcc ggtctttccg cccgctccga actgtcttcg
     1741 gtatcgcccg gcactttcaa ggaggtgcgc ggcggccgtc atgtctattt catcgagcgt
     1801 ctcgacgacg ttgcggggaa gatcggcaat gttttcgtcg cttcgctgca ggagggaaag
     1861 cttggtgtcg tcatgtccga ttccggagcc caggaagtcc atgaaaatgg cagccgtttt
     1921 ctggttctgc agcgtgggcg gcgctatgaa gtcgaaccgg ggagcgcggc tttccggatc
     1981 atggact
//
LOCUS       DAOSDX010000583         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19545_length_1985_cov_3.522798, whole genome shotgun sequence.
ACCESSION   DAOSDX010000583 DAOSDX010000000
VERSION     DAOSDX010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19545_length_1985_cov_3.522798"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLN02_11820"
     CDS             complement(<1..356)
                     /locus_tag="PLN02_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53059.1"
                     /translation="MQFSPLDVVMADARSNPSEIAREALKRLAERRVAPTPANYQACY
                     FEIARLPNVLPFPEPALRQLAGALCAHGDAQAAQLARLDSAISGRSWQGVQEALLGYI
                     AAGQASSGAANLPHETT"
     gene            508..>1985
                     /gene="ubiB"
                     /locus_tag="PLN02_11825"
     CDS             508..>1985
                     /gene="ubiB"
                     /locus_tag="PLN02_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HOI53060.1"
                     /translation="MRLFRLLKIAAVASRFGLDEMVLEHEPSGRLVRLANALQFWRNL
                     SAPRAERLRLALETLGPIFVKFGQVLSTRRDLMPADIADELAKLQDRVPPFSSAQALA
                     EIEKAYGRPASEVFAEFDPVPVASASIAQVHFARLHERDGGHEVAVKILRPNMLSVIE
                     HDLALMDSGAMLLEKLWADGRRLKPREVVAEFAKYLRDELDLMREAANASQLRRNFTD
                     SKLLLVPEVYWDFCTSTVMVMERMHGTPISQTARLVADGIDMKKLSEAGVEIFFTQVF
                     RDGFFHADMHPGNIFVAADGRYIALDFGIVGTLTDTDKNYLAQNFLAFFRRDYKRVAE
                     THIESGWAPRDTRVDEFEAAIRAVCEPIFDRPLRDISFGKVLLRLFQTSRRFNVEIQP
                     QLVMLQKTLLNIEGLGRQLDPDLDLWQTAKPFLERWMSEQIGWRGLLKSFQQEAPQLA
                     RTLPQLPRLVHQALAAPRHADPQPQLERLIAAQRRQNLLLAI"
BASE COUNT          340 a          688 c          617 g          340 t
ORIGIN      
        1 gtcgtttcgt gcggcaggtt cgccgcgcca ctgctcgcct gaccggcggc gatatagccg
       61 agcaatgcct cctgcacgcc ctgccagcta cgcccgctga tcgccgaatc cagtcgtgcc
      121 agttgcgccg cctgggcatc gccgtgggcg cacagtgcac cggccagttg gcgcaacgcc
      181 ggctcgggga aaggcaggac gttgggcagg cgggcgattt cgaaataaca ggcctggtag
      241 ttggccgggg tcggcgcgac ccggcgctcc gccaggcgtt tcaaggcttc gcgggcgatt
      301 tcggagggat tgctgcgtgc atcggccatg acgacatcca gtggggagaa ctgcattgcg
      361 gttgtactcg gcaaccgttc gttcacacca agtttatccg aagcccgcgc gctggcggac
      421 ggcattcgcc gtttaccgca gatgcggaca acacagggcg cgcggcgtgt taacatgccc
      481 gtttgaccaa tctggcccgc cccgccgatg cgccttttcc gcctgctcaa gatcgctgct
      541 gtagccagcc gtttcggcct tgacgaaatg gtgctcgagc acgagccgtc gggccgtctc
      601 gtccgcctgg ccaacgcgct gcagttctgg cgcaacctgt cggcaccgcg cgccgagcgc
      661 ctgcgcctgg cgctggagac gctggggccg atcttcgtca agttcggcca ggtgctatcc
      721 acccgccgcg acctgatgcc ggccgacatc gccgatgaac tggccaagct gcaggaccgg
      781 gtgccgccct tctcctcggc gcaggccctg gctgagatcg agaaggccta cggccggccg
      841 gcgagcgagg tttttgcgga attcgacccg gtgccggtgg cctcggcgtc gatcgcccag
      901 gtgcatttcg ccaggctgca cgaacgcgac ggcggccacg aagtagcggt caagatcctg
      961 cgcccgaaca tgctgtcggt gatcgagcac gatctggcgc tgatggacag cggcgccatg
     1021 ctgctggaaa agctgtgggc cgacggccgc cgcctcaagc cgcgcgaagt cgtcgccgaa
     1081 ttcgccaagt acctgcgcga cgaactcgat ctcatgcgcg aagcggccaa cgcctcgcaa
     1141 ctgcggcgca acttcaccga ttcaaagctg ctgctggtac ccgaggttta ctgggatttc
     1201 tgcacctcga ccgtgatggt catggaacgc atgcacggca cgccgatctc gcagaccgcg
     1261 cgtctggtcg ccgacggcat cgacatgaag aaactgtccg aagccggcgt cgagatcttc
     1321 ttcacccagg tcttccgcga cggtttcttc cacgccgaca tgcaccccgg caacatcttc
     1381 gtcgccgccg acggccgtta catcgcgctc gacttcggca tcgtcggcac gctgaccgac
     1441 accgacaaga actacctggc gcagaacttc ctcgccttct tccgccgcga ctacaagcgc
     1501 gtcgccgaaa cccacatcga atccggctgg gcgccgcgcg acacccgggt cgacgagttc
     1561 gaggcagcga tccgcgccgt ctgcgagccg atcttcgacc ggccgctgcg tgacatttcc
     1621 ttcggcaagg tgctgctgcg gctgttccag acctcgcgcc gcttcaacgt cgagatccag
     1681 ccacaactgg tgatgttgca gaagaccctg ctcaacatcg aaggcctcgg ccgccagctc
     1741 gacccagacc tcgacctgtg gcagaccgcc aagcccttcc tcgaacgctg gatgagcgaa
     1801 cagatcggct ggcgcggttt gctgaagagc ttccagcagg aggcgccgca actggcacgc
     1861 acgctgccgc aactgccacg cctcgtgcat caggcgctgg cggcaccgcg tcacgccgat
     1921 ccgcagccac aactggaacg cctgatcgcc gcccagcgcc ggcagaacct gttgctggcg
     1981 atcat
//
LOCUS       DAOSDX010000584         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19651_length_1977_cov_3.274714, whole genome shotgun sequence.
ACCESSION   DAOSDX010000584 DAOSDX010000000
VERSION     DAOSDX010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19651_length_1977_cov_3.274714"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLN02_11830"
     CDS             complement(<1..190)
                     /locus_tag="PLN02_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOI53061.1"
                     /translation="MKIEEMIREVQQELGVGVDGKAGPETWGAIHARIVKPKAAAPAV
                     SIAVSPVDPRSEKNILTLL"
     gene            392..757
                     /locus_tag="PLN02_11835"
     CDS             392..757
                     /locus_tag="PLN02_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HOI53062.1"
                     /translation="MNPASPSQPTLFGIKNCDTMKKAMHWLSENGVAYEFVDYKKAGV
                     AAAKLPDWAARAGWQKLLNTRGLMWKKLSEAERSDVDEAKALQLMTQYPALIKRPVLD
                     LGGELLVGFSPESYAEKLK"
     gene            754..1635
                     /locus_tag="PLN02_11840"
     CDS             754..1635
                     /locus_tag="PLN02_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOI53063.1"
                     /translation="MSGGNVRRFLFEGLDIRGAVVHLDEAWQQMQVGRDYQPTVAQLL
                     GEVAAVTALIAGQLKQPGRLTLQLRGSGPVQLLVMDCNEALQMRGMARSNPVVLPAPV
                     PELLGAQQGGQLLMSLDLPTARQPYQSYVPLVGESIAAIFEHYLEQSEQQPSRLFCTA
                     APQAAACLFLQKMPEADKHDADGWQRVTQLASTVKPAELLTLDAETLLGRLFHEEIAA
                     GGVRVYDPQPVVYHCPEDWDKVRDMIRGIGRADAETILAEHGEILIRDDICNRDYRFT
                     AADVAAIFAASEGKALN"
     gene            complement(1645..>1977)
                     /locus_tag="PLN02_11845"
     CDS             complement(1645..>1977)
                     /locus_tag="PLN02_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI53064.1"
                     /translation="DVDNSIFFASSGNTIDAEGRQRLLEHAARLKADPELVVTLVGHA
                     DHLGSRSYNLAIAEQRINAVFAVLRGAGIPVTQIRREAVGSEEVSPACRSPQCRKLMR
                     RVQLVYQK"
BASE COUNT          330 a          615 c          681 g          351 t
ORIGIN      
        1 ggagcagcgt caggatgttc ttttcgctac gcgggtcgac cggtgagacg gcgatgctga
       61 ccgccggagc cgccgctttc ggcttgacga tgcgggcgtg gatggcgccc caggtttccg
      121 gtccggcctt gccgtcgacg ccgacgccga gttcctgctg gacttcccgg atcatttctt
      181 cgatcttcat ttttcgctcc tccagggttc aaatggggtt tcccccgttg ttcttcagaa
      241 acgatatagc cggatgtcat gatgccatct tgctaccgga aattcccgcc ttttttgccg
      301 ccagcgtatg gctgcggccg ccggaagcgg gctgctatca ttgcgccttg tcgcgggccc
      361 ggcccgcttc cttacctgca gagtcccgga catgaaccca gcctccccga gccagccgac
      421 cctgttcggc atcaagaatt gcgacacgat gaagaaggcc atgcactggc tgagcgagaa
      481 cggcgtcgcc tacgagttcg tcgattacaa gaaggccggc gtcgccgccg ccaagctgcc
      541 ggactgggcg gcgcgcgccg gctggcagaa actgctcaac acgcgcggcc tgatgtggaa
      601 gaagctgtcc gaggcggagc gcagcgatgt cgacgaagcg aaggcgttgc agttgatgac
      661 gcagtatccg gcgctgatca agcggccggt gctcgatctg ggcggcgaac tgctggtcgg
      721 cttcagcccg gaaagctatg cggagaagct gaaatgagtg gcggcaacgt tcgtcgcttc
      781 ctgttcgagg ggctggatat ccgcggtgcc gtggtgcatc tggacgaagc ctggcagcag
      841 atgcaggtgg gccgcgatta ccagccgacg gtggcgcaac tgctcggcga ggtggcggcg
      901 gtgacggcgc tgatcgccgg ccagttgaag cagcccggcc ggctgacact gcagttgcgc
      961 ggcagcgggc cggtgcaact gctggtgatg gattgcaacg aggcgctgca gatgcgcggc
     1021 atggcgcgca gcaacccggt ggtgctgccg gcgccggtgc cggaattgct cggcgcgcag
     1081 cagggcggcc agctattgat gagcctggac ctgccgaccg cgcgccagcc ttaccagagc
     1141 tatgtgccgc tggttggcga gagcatcgcg gcgattttcg aacactacct ggagcaatcg
     1201 gagcagcagc cgtcgcggct gttctgcacg gcggcgccac aggctgcggc ctgcctgttc
     1261 ctgcagaaga tgccggaggc ggacaagcac gatgccgacg gctggcagcg cgtgacccaa
     1321 ctggcgtcga cggtgaagcc ggcggaactg ctgacgctgg atgccgagac cctgctcggc
     1381 cgcctcttcc acgaggagat cgccgctggc ggcgtgcgcg tctatgaccc gcagccggtg
     1441 gtctatcact gcccggaaga ttgggacaag gtgcgcgaca tgatccgcgg cattggccgc
     1501 gccgacgccg agacgatcct tgccgaacac ggcgaaatcc tcatccgtga cgatatctgc
     1561 aaccgcgatt accgcttcac ggcagcggac gtggcggcga tcttcgccgc cagcgagggg
     1621 aaggcgctca actgagcgcc ccgcttactt ctggtaaacc agctgcacgc gccgcatcag
     1681 cttgcggcat tgcggcgaac ggcaagccgg gctgacttct tccgagccga ccgcctcgcg
     1741 ccggatctgg gtcaccggaa tgccggcgcc gcgcagtacg gcgaagacgg cgttgatccg
     1801 ctgttcggcg atggccaggt tgtaggagcg gctgcccagg tggtcggcat gcccgaccag
     1861 ggtgacgacc agttccgggt cggccttcag gcgggcggcg tgttcgagca ggcgctgccg
     1921 cccttcggcg tcgatcgtgt tgcctgacga cgcaaagaag atgctgttgt cgacatc
//
LOCUS       DAOSDX010000585         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19652_length_1977_cov_3.108741, whole genome shotgun sequence.
ACCESSION   DAOSDX010000585 DAOSDX010000000
VERSION     DAOSDX010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19652_length_1977_cov_3.108741"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLN02_11850"
     CDS             <1..202
                     /locus_tag="PLN02_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOI53065.1"
                     /translation="EPGDVEAALTAASAIGDDRLQQQAQGRIVPDSFTHGSSAQRMRW
                     FQRGFDSGDFRQCNTFDKSARL"
     gene            204..728
                     /locus_tag="PLN02_11855"
     CDS             204..728
                     /locus_tag="PLN02_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HOI53066.1"
                     /translation="MRADTTGKPARWRRWALEIAIFVAAFLAFQAWQLRDTARGPAPQ
                     FTGMHTDGQAFDLAAWRAAHPGQPLLIYFWAEWCPICKTTAGSVGNIANDHPTITVAT
                     QSGDGAAVARFMADRGYRFPTLADPDGRIMQTYRLPGTPGFVILAPDGHIRFVSVGYT
                     SELGLRLRLWWAGL"
     gene            complement(730..1431)
                     /locus_tag="PLN02_11860"
     CDS             complement(730..1431)
                     /locus_tag="PLN02_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI53067.1"
                     /translation="MLRVSGLESCYGRIKALKGIDLEVRQGELVALVGANGAGKTTFL
                     RALSGVQPISAGRVFFDGDDITNLRADQRVRRGICQSPEGRQVFGPLSIEDNLRLGAF
                     TQPKAQVEGDLDKVFAMFPILKEKRHLPAGTLSGGQQQMLAIGRALMGRPKMLLLDEP
                     SMGLAPLLVEEVFNVVKSLKAQGMTILLVEQNAFAALAIADRGYVLETGAVTLTDTGQ
                     ALIANEQVRAAYLGM"
     gene            complement(1648..1956)
                     /locus_tag="PLN02_11865"
                     /pseudo
     CDS             complement(1648..1956)
                     /locus_tag="PLN02_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
BASE COUNT          290 a          709 c          671 g          307 t
ORIGIN      
        1 cgaaccgggc gacgtcgagg ccgcgctgac cgccgcttcg gccatcggcg acgaccgcct
       61 gcagcaacag gcgcagggtc gcatcgtccc ggacagcttc acgcacggca gctcggcgca
      121 gcgcatgcgc tggttccagc gcggtttcga cagcggcgac ttccgccagt gcaacacctt
      181 cgacaagagc gcgcggctct gagatgcgcg ccgacacaac cggcaagcca gcgcgctggc
      241 gccgctgggc gctggagatc gccatcttcg tcgccgcctt cctcgccttc caggcctggc
      301 agttgcgcga cacggcgcgc ggaccggcgc cgcaattcac cggcatgcac accgacggcc
      361 aggccttcga cctcgccgcc tggcgcgccg cccaccccgg ccagccgctg ctgatctatt
      421 tctgggccga atggtgcccg atctgcaaga ccaccgccgg cagcgtcggg aacatcgcta
      481 acgaccaccc gacgatcacc gtcgccacgc agagcggcga cggcgcggca gtcgcccgct
      541 tcatggcgga ccggggctac cgcttcccga ccctggccga ccccgacggc cgcatcatgc
      601 agacctaccg cctgcccggc acgccgggct tcgtcatcct cgcgccggac ggccacatcc
      661 gcttcgtctc ggtcggctac accagcgagc tcggcctgcg cctgcgcctg tggtgggccg
      721 gcctctgact tacatgccga ggtaggcggc gcgcacctgt tcgttggcga tcagcgcctg
      781 gccggtgtcg gtgagcgtca ccgcgccggt ttccagcacg tagccgcggt cggcgatggc
      841 cagcgcagca aaggcgttct gctcgacgag caggatggtc atgccctgcg ctttcagcga
      901 cttgacgacg ttgaacactt cctcgaccag caagggcgcc aggcccatcg acggctcgtc
      961 gagcagcagc atcttcggtc gccccatcag cgcgcggccg atcgccagca tctgctgctg
     1021 gccgccggac aaggtgccgg ccggcaggtg gcgcttttcc ttgaggatgg ggaacatcgc
     1081 gaagaccttg tcgaggtcgc cctcgacctg cgccttcggc tgcgtgaagg cccccagacg
     1141 caggttgtcc tcgatcgaca gcgggccgaa cacctgccgc ccctccgggc tctggcagat
     1201 gccgcggcgc acgcgctggt cggcgcgcaa gttggtgatg tcgtcgccgt cgaagaaaac
     1261 gcgcccggcg ctgatcggct ggacacccga cagggcgcgc aggaaagtgg tcttgccggc
     1321 gccgttggcg ccgaccaggg cgaccagctc gccttggcgc acttcgaggt cgatcccctt
     1381 gagcgccttg atccgcccgt agcagctctc caggccgctg acgcgcagca tggtgtcgcc
     1441 ggtcacggca tgcgccgccg gctccaggtc gccgccgctg tgcgcgccga ggccgaccga
     1501 ttccggcgcc aggctggcga tgcggtggaa gagctggcga tattcgaggc gcgcctgttc
     1561 gccgaacacc ggatcggcgt tgtcgaggaa ggcgcggtcg atgaccgccc agtcctcggg
     1621 caggagcact tcgcgggcga gcggcatggc gtccttttcc tcggtccgga tgtgcgactt
     1681 caggctctcg gcgaagatgc gcaagttgcc ggcgaattcg cccggcgcgc tggccggcgc
     1741 cgccagctgg cggcgcagcg aacggaggat ttccggcccg ttgcggtgtt cgatctgcag
     1801 gcgatcgagc accgccgccg cttccgggct gcgctggcgc agcgccggga acaggtattc
     1861 gtcctccttg gcgtggtggc aggcgtccat gaagtgttcg atgtagtcga acaccgagtg
     1921 gaagaaggcc ggatcgaccg gctcgccggc ttccatctcg ccagcgacga ggtcgag
//
LOCUS       DAOSDX010000586         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19668_length_1976_cov_2.357106, whole genome shotgun sequence.
ACCESSION   DAOSDX010000586 DAOSDX010000000
VERSION     DAOSDX010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19668_length_1976_cov_2.357106"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLN02_11870"
     CDS             <1..412
                     /locus_tag="PLN02_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOI53068.1"
                     /translation="LLGQGDMLYLAPGTGYPTRVHGAFVSDDEVHRVVEHLKANGAPE
                     YVEDILSGAGGDEEGGGEAGEGGGDAESDPLYDQAVDIVLKNQRASISLVQRHLRIGY
                     NRSARLIEAMENAGLVSAMDGRGGREVLMKKPAE"
     gene            482..1099
                     /gene="lolA"
                     /locus_tag="PLN02_11875"
     CDS             482..1099
                     /gene="lolA"
                     /locus_tag="PLN02_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HOI53069.1"
                     /translation="MNLTRKLLAAAFVLAPAFAHAGAIDQLRAFLDDTRSYRADFDQS
                     VLARNGRKPQESSGKLAILRPGKLRWEILKPYPQLVVGDGEKIWIHDPELQQVTVRPA
                     GDAIGASPAALLAGSNALEKNFVLREGGERDGLSWVEATPKTAESGFEKVRIGLLGKE
                     MKVMELFDNFGQTTLIRLQRSERNPALPATLFRFTPPAGSDVIGQ"
     gene            complement(1086..1559)
                     /locus_tag="PLN02_11880"
     CDS             complement(1086..1559)
                     /locus_tag="PLN02_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53070.1"
                     /translation="MTVPLPEVLTWAGSGLALLVVGILLFRNRLPSRAPDFLGGGVGG
                     IGFMLIVYGALLLLVFAPSEYFVERIVVSPSSTCAARGFWFWRQPPLRLEHADIVSLR
                     RTSDAKGDPQLEVRLRDGSLQRKWLPSLWTDNEAQIEAAWRRLGIGSEWGPLTDR"
     gene            complement(1615..>1976)
                     /locus_tag="PLN02_11885"
     CDS             complement(1615..>1976)
                     /locus_tag="PLN02_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOI53071.1"
                     /translation="PCALRPGVPGLSDNIRVRSVIGRFLEHHRIFHFHADGDDVMFLS
                     SADWMDRNFFKRIELGFPLLDPRLKKRVITEGLKFYLADNQQGWDMNSQGHYQRRRSL
                     RSKPHNAQGELMVTLGG"
BASE COUNT          355 a          649 c          670 g          302 t
ORIGIN      
        1 gctgctcggc cagggcgaca tgctttatct cgcgccgggt accggctatc cgacccgcgt
       61 ccatggcgcc ttcgtttccg acgatgaagt gcatcgcgtc gtcgagcacc tcaaggcgaa
      121 cggcgccccg gaatacgtcg aggacatcct gagcggcgcc ggtggcgacg aggaaggcgg
      181 cggcgaggcc ggcgaaggtg gcggcgacgc cgagagcgat ccgctctacg accaggcggt
      241 cgatatcgtg ctgaagaacc agcgcgcttc gatctcgctg gtgcagcgcc acctgcgtat
      301 cggctacaac cgctcggcgc ggctgatcga ggcgatggaa aacgccggcc tggtgtcggc
      361 gatggacggt cgcggcggtc gcgaagtgct gatgaagaag ccggccgaat gacgcggccg
      421 gcgcgacaaa cccttacaca ttgaaacagc gccggcccac aggccggcgt aagctgcgcc
      481 catgaacctc acccggaaac ttctcgctgc cgccttcgtc ctcgctccgg ccttcgccca
      541 cgccggtgcc atcgaccagt tgcgcgcctt cctcgacgac acccgcagct accgcgccga
      601 cttcgaccag tcggtgctcg cccgcaacgg ccgcaagccc caggaatcct cgggcaagct
      661 ggcgatcctg cggccgggca agctgcgctg ggaaatcctc aagccctatc cgcaactggt
      721 ggtcggcgac ggcgaaaaga tctggattca cgatccggaa ctgcagcagg tcacggtgcg
      781 cccggccggc gacgcgatcg gcgcctcgcc ggcggcgctg ctcgccggca gcaatgcgct
      841 ggaaaagaac ttcgtgctca gggaaggcgg cgagcgcgac ggcctctcgt gggtcgaggc
      901 gacgccgaag actgccgaga gcggcttcga gaaagtgcgc atcggcctgc tcggcaagga
      961 aatgaaggtg atggagctgt tcgacaactt cggtcagacg acgctgatcc ggctgcagcg
     1021 cagcgagcgc aacccggcgc tgccggcaac gctgttccgc ttcacgccgc ccgccggcag
     1081 cgacgtcatc ggtcagtaag cggcccccat tcgctgccga tgccgagccg ccgccaggcg
     1141 gcttcgatct gcgcttcgtt gtcggtccac agggacggta gccacttgcg ctgcaggctg
     1201 ccgtcacgca gccggacttc cagttgcgga tcgcccttgg cgtcgctcgt ccggcgcaag
     1261 ctgacgatat cggcatgctc gaggcgcagt ggtggctgtc gccagaacca gaaaccacgc
     1321 gccgcgcagg tgctgctcgg cgagacgacg atgcgctcga cgaaatactc gctgggggcg
     1381 aaaaccagca acagcagggc gccgtacacg atgagcatga agccgatacc gccgacacca
     1441 ccgccgagga aatcgggggc gcgcgacggc agccggttgc ggaacagcag tatgcctacg
     1501 accaggagcg ccagaccgct gccggcccag gtcaggactt ccggcagcgg cacagtcaag
     1561 atcagatcgg cgcccgcgct ggggcaggat tgcgtcatcg ctgacttggt ggagtcagcc
     1621 gcccagcgtc accatcagtt cgccctgcgc attgtgcggt ttgctgcgta gcgagcggcg
     1681 gcgctgatag tgtccctggc tgttcatgtc ccagccttgc tggttgtcgg ccaggtagaa
     1741 cttgagccct tcggtgatca cgcgcttctt caggcgcggg tcgagcagcg ggaagccgag
     1801 ttcgatgcgc ttgaagaagt tgcggtccat ccagtcggca ctcgacagga acatcacgtc
     1861 atcgccgtcg gcgtggaagt ggaagatgcg gtggtgctcg aggaagcggc cgatcaccga
     1921 gcgcacgcgg atgttgtccg aaaggcccgg tacacccggc cggagcgcgc agggtc
//
LOCUS       DAOSDX010000587         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19731_length_1971_cov_3.883612, whole genome shotgun sequence.
ACCESSION   DAOSDX010000587 DAOSDX010000000
VERSION     DAOSDX010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19731_length_1971_cov_3.883612"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /gene="aceK"
                     /locus_tag="PLN02_11890"
     CDS             complement(<1..1410)
                     /gene="aceK"
                     /locus_tag="PLN02_11890"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOI53072.1"
                     /translation="MKISVGLYGTNAHQIALALIQGFNRHYSLFRQTCHEAKTRFEQG
                     DWLGVHRAVKERIRFYDDRVDECVDRLRSEFDARNIDDSVWQQVKLLYIGLLLNHKQP
                     ELAETFFNSVTTKILDRTYFHNDFLFVRPTISTENLEADSDPIYRSYYAKANGLRGAI
                     REIVEDFGWQRPFADLERDVDYVYHSIRRYLKEMPRREVNFQIQVLGSAFYRNKAAYI
                     IGKAVNGALEYPFAIPVLHDAEGHLYLDTVLLDAWRIGLLFSLSRAYFMVDMEVPSGY
                     VQFLRSIMPNKPRSEIYVMLGLGKQGKTMFFRDFIYHLHHSEDEFIMAPGIRGLVMLV
                     FTLPSFPYVFKIIKDIFGSSKEMDQATVKKKFLMVKQVDRVGRMADTLEFSNVMFPLK
                     RFDDEVMDELRRLAPSKFEIDGEQLIIKHLYIERRMEPLNIHLERMDKSNNLAGLEHA
                     ITEYGSAIRELAQANIFPGD"
     gene            1614..>1971
                     /locus_tag="PLN02_11895"
     CDS             1614..>1971
                     /locus_tag="PLN02_11895"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOI53073.1"
                     /translation="MSAEESKIIYTLTDEAPLLATCAFLPVIRTFTAPAGIAIEKADI
                     SVSARVIAEFPEYLTEAQRLPNTLGELGKKCLEPETNIIKLPNISASVAQLKACIKEL
                     QEKGYALPDFPENPASD"
BASE COUNT          386 a          559 c          618 g          408 t
ORIGIN      
        1 gtcgccgggg aagatgttgg cctgcgccag ttcgcggatg gcgctgccgt attcggtgat
       61 cgcgtgctcc aggccggcca ggttgttcga cttgtccatc cgttcgaggt ggatgttgag
      121 cggctccatc cggcgttcga tgtagaggtg cttgatgatc agttgctcgc cgtcgatttc
      181 aaatttcgac ggggcgagcc ggcgcagttc gtccatcacc tcgtcgtcga agcgcttgag
      241 cgggaacatc acgttggaga actccagcgt gtcggccatc cggccgacgc ggtcgacctg
      301 cttgaccatc aggaacttct tcttgaccgt cgcctggtcc atctccttgg agctgccgaa
      361 gatgtccttg atgatcttga acacgtaggg gaaggagggc agcgtgaaca ccagcatgac
      421 caggccacgg atgccgggcg ccatgatgaa ctcgtcttcc gagtggtgca ggtgatagat
      481 gaagtcgcgg aagaacatcg tcttgccctg cttgccgagg ccgagcatga cgtagatttc
      541 cgagcgcggc ttgttcggca tgatcgagcg caggaactgc acgtagcctg acggcacttc
      601 catgtcgacc atgaaatagg cgcgcgacag cgaaaacagc aagccgatac gccaggcgtc
      661 gagcagcacg gtgtcgaggt agaggtgtcc ttcagcgtca tgcagcacgg gaatggcgaa
      721 agggtattca agcgcgccgt tgaccgcctt gccgatgatg taggccgcct tgttgcggta
      781 gaaggccgaa ccgagcacct ggatctggaa gttgacctcg cgccgcggca tctccttcag
      841 gtagcggcgg atgctgtggt agacgtagtc gacgtcgcgt tcgaggtcgg cgaacgggcg
      901 ctgccagccg aaatcctcga cgatctcgcg gatcgcgccg cgcaggccgt tggccttggc
      961 gtaatagctg cggtagatcg gatcgctatc ggcttccagg ttttccgtcg agatggtcgg
     1021 ccggacgaac aggaaatcgt tgtgaaaata ggtccggtcg agaatcttgg tggtcaccga
     1081 attgaagaag gtttccgcca gctccggctg cttgtggttg agcagcaggc cgatgtagag
     1141 cagcttgacc tgctgccaga cgctgtcgtc gatgttgcgc gcgtcgaact cgctgcgcag
     1201 gcgatcgacg cattcgtcga cgcggtcgtc gtagaagcgg atgcgttcct tgaccgcgcg
     1261 gtgcactccg agccagtcgc cttgttcgaa acgcgtcttg gcttcgtggc aggtttggcg
     1321 gaacagcgaa tagtgccggt tgaagccctg gatcagcgcg agcgcgatct ggtgcgcgtt
     1381 ggtgccgtaa agaccgaccg aaatcttcat cgtccattcc cccggaagtg cggtcgattc
     1441 tagcatcggc atcgattgcc gctgcccgct gcgcaatatg aaatgcagtt tcaccggttg
     1501 accgatcggc tggcgttgcg gatactccca gggtttcgta agcactgcgc aagggtgtcg
     1561 agctaagctt cacccgcata ttgataaaag ccatcgcaaa ggggttagtt agcatgtctg
     1621 cagaggaatc caagatcatc tatacgctta ccgacgaggc gccgctgttg gcaacgtgcg
     1681 ccttcctgcc ggttatccgc acgttcacgg cgcccgccgg gatcgccatc gagaaggccg
     1741 acatttcggt atcggcccgc gtcatcgccg aatttcccga atacctgacg gaagcgcaac
     1801 gtttgccgaa caccctcggc gaactcggca agaagtgtct cgagcccgaa accaacatca
     1861 tcaagctgcc caacatctcg gcctcggttg cccagttgaa ggcttgcatc aaggaacttc
     1921 aggaaaaggg ctacgccctg cccgatttcc cggaaaatcc ggccagcgac g
//
LOCUS       DAOSDX010000588         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19737_length_1971_cov_2.963466, whole genome shotgun sequence.
ACCESSION   DAOSDX010000588 DAOSDX010000000
VERSION     DAOSDX010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19737_length_1971_cov_2.963466"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..>1971)
                     /locus_tag="PLN02_11900"
     CDS             complement(79..>1971)
                     /locus_tag="PLN02_11900"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI53074.1"
                     /translation="GKRATTEAGRLRRRQRLSAQASGIAATKHAPLSPGAEVNRLVYL
                     HSSDAAIVIRVSGGSFLLEDANLVAQEVLHGESRFFCGQGKLPPIVLDPGLSASVARL
                     VEACERCLHTTLPVAYDGTLSLSGETSQRLFRIHLNPLADDSGIARIIAIGHDITVAR
                     AHEIALLQRAETEARMSSYFRSAPGDFFTIACAGDGKWSLLFASPGLAATLARSGCAD
                     FQRPSELLCLLHPECAVRVEENLRAARSDSRPAVIECRLAEGGDDSERWIELRCTPRQ
                     LADGGVAWDGVVLDISARKKIESALTVSEQAYRALIENAPDPILRFDRNGAILYANPA
                     ARAQLGSILHSGSRIYEFSERALGNARETRGVFEAVSERGKPRHFQCRAVPEMDENGK
                     PISALLIARDVTEMKESASRLAESGEQLRLLVNHGDVIREEERKRLARDIHEELGQLL
                     TSLHLGLSSLALEFGKKLAGLDKKVGELLSLVAISLQSTRDLVSSLRPTALNSGIESG
                     LVWLAENFLRYNHVRPKLQITPNTKGLTENQATALFRIAQEALTNISRHAAASEVSLA
                     LRREAGYWCFEVRDNGKGFEASERNTKRFGLLGISERAVALGGRAEVVSKPGRGTRIL
                     VRIPFA"
BASE COUNT          371 a          648 c          600 g          352 t
ORIGIN      
        1 ggggtaggct catccctacc caagcgctcg ccgccgccct tggcatgcgt cccctgcggt
       61 caggaatttc gagccatctc aggcaaaggg aatcctgacc agaatccggg ttccacggcc
      121 cggtttgctc accacctcgg cgcgcccccc cagggcaacc gccctttcgg agattcccag
      181 caagccgaaa cgctttgtgt tccgctcgga ggcctcgaag cccttgccgt tgtcccgaac
      241 ctcgaagcac cagtagccgg cttcgcggcg cagggccagg ctgacttcgc tggcggcggc
      301 atggcgggag atattggtca gcgcctcctg ggcgatccgg aacagcgccg tcgcctgatt
      361 ctcggtgagc cccttggtat tcggcgtgat ctgcagcttg gggcgaacgt ggttatagcg
      421 caggaagttt tccgccagcc agacgagacc gctttcgatc ccggaattga gggcggtcgg
      481 acgcagggag gaaaccaggt cgcgggtgct ctgaagactg atcgcgacca gggaaagaag
      541 ctcccccacc tttttgtcca gccccgccag cttcttgccg aattcgaggg ccaaggacga
      601 aagtcccaga tgcagcgagg tcaggagttg cccgagctct tcgtgaatgt cccgggccag
      661 acgcttcctc tcctcttcgc gaatgacatc gccgtgattg accagcagcc tgagctgctc
      721 gccggattcc gccagacggc tggcactctc cttcatttcc gtgacatcgc gcgcgatcag
      781 cagcgcactg atcggcttgc cgttctcatc catttcgggc acggcacggc actggaaatg
      841 gcgaggtttg ccgcgctccg aaaccgcctc gaaaacgccg cgggtctcac gggcgttgcc
      901 cagcgctctt tccgaaaact cgtagatgcg cgaaccgctg tgcagaatgc tacccagttg
      961 cgcacgggcg gcgggattgg cgtagaggat cgcgccattt cggtcgaagc ggagtatcgg
     1021 gtcgggagca ttctcgatca gtgcccgata agcctgttcg ctgacggtca gcgccgattc
     1081 gatctttttc cgcgcactga tatcaagaac cacgccgtcc caagccaccc caccatcggc
     1141 taactgtcgc ggcgtacagc gcagttcgat ccagcgctcg ctgtcgtcgc cgccctcggc
     1201 cagacgacat tcgatgaccg ctggccggct gtcgctccgg gcggcacgca ggttttcttc
     1261 aacacgcacg gcgcattccg gatggagcaa gcacaacagt tcagaggggc gctggaaatc
     1321 ggcgcaacca gaacgcgcca acgtcgcggc aagccccgga ctggcaaata acaacgacca
     1381 cttgccgtcc ccggcacagg cgatggtgaa aaagtcgccg ggggcgctac ggaaatagct
     1441 ggacatgcgc gcctcggttt cagctcgctg cagcagcgcg atttcgtgcg ccctggccac
     1501 cgtgatatcg tgcccgatcg cgatgatccg agcaattccg ctatcatcgg caagcgggtt
     1561 cagatgaatc ctgaacagtc gttgcgaagt ctccccggac aaactcaggg tgccatcgta
     1621 ggccaccggc agcgtggtgt gaaggcaacg ctcgcaagcc tcaaccaagc gggcgacgct
     1681 cgccgacaga ccgggatcga gcacgatcgg cggaagcttg ccttggccgc aaaagaaccg
     1741 actttcccca tgcagaacct cctgcgccac caggttggcg tcttccagca ggaagctccc
     1801 gccactgaca cgaatgacga tggccgcgtc ggatgaatgc aggtagacca ggcgattgac
     1861 ttctgctccc ggcgaaagcg gggcgtgctt ggtggcggca atgcccgacg cttgagcgga
     1921 cagccgctgc cgccggcgca gcctccccgc ctcggtggta gcgcgcttcc c
//
LOCUS       DAOSDX010000589         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19749_length_1970_cov_2.738381, whole genome shotgun sequence.
ACCESSION   DAOSDX010000589 DAOSDX010000000
VERSION     DAOSDX010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19749_length_1970_cov_2.738381"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="PLN02_11905"
     CDS             complement(<1..1771)
                     /locus_tag="PLN02_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOI53075.1"
                     /translation="MKEERRHIVALVLLPTLLSLALLVFFLRASLLDWAVARWTEDNR
                     AFVAALAARLDAEIDQPLQLLRLAAQTRAFSDLPALAQIDRNINGIPENVDPEKREIL
                     ESLRRQGNFSVLFVLTPEGDHYISHPFSVQRALRKYNLADRRYFRDAASSGEAVVSGG
                     FLGADGVPAVAIDVPVRDASGRIVLHLGGVIHLSRLADLLVAERIAPFEHAALFDAAG
                     GKVASSRVGASTGQAPDHSLAGKDGVERVSDASGEPWLRFVVPLAHGWHLSLARSERA
                     LSAAIAPQVQRVIQSVALMVLVPCLIGMLLAFRFSRRWRRADHALHEANADLERRVAE
                     RTEALQVSEERYRSLFESTAESVLILDGHAIVDCNPGALALFGAASREALRGRSPLDL
                     SPPLQPGGEPSRELAGRLIEAALQGSFPRFEWTHRRLDNGRPFVAEIMLGRIQSGGRT
                     LIQCSLRDISERRRIEAELAAYREHLEELVELRTGELAQAKQAAESASIAKSAFLANM
                     SHEIRTPMNAILGMTRLVRRGGLSDGQAAQMDKLEIASGHLLEIINDVLDLSKIEAGK
                     FVLESLPVQPAAIVANVLSMIHER"
BASE COUNT          323 a          670 c          673 g          304 t
ORIGIN      
        1 cgcgttcgtg gatcatcgac aagacgttgg cgacgatcgc cgccggctgc accggcagcg
       61 actcgaggac gaacttgccg gcctcgatct tcgacaggtc gagcacgtcg ttgatgattt
      121 cgagcaggtg gccgctggca atctccagct tgtccatctg cgccgcctgc ccgtcgctca
      181 gcccacccct gcgcaccagc cgggtcatgc cgaggatggc gttcatcggc gtgcggattt
      241 cgtggctcat gttggccagg aaggcgctct tggcgatgct ggcgctctcc gccgcctgct
      301 tggcctgcgc cagttcgccg gtgcgcagtt cgaccagttc ctcaaggtgt tcgcggtagg
      361 ccgccagttc ggcctcgatc cgccgccgtt cggaaatgtc gcgcaggctg cactggatca
      421 gcgtccgccc gccggactga atgcggccga gcatgatttc ggcgacgaaa ggacggccgt
      481 tgtcgagccg gcggtgggtc cattcgaaac gcgggaaact gccctgcagc gccgcctcga
      541 tcagccgccc ggccagttcg cgggacggtt cgccgcccgg ctgcagcggc ggcgacagat
      601 cgagcggcga acggccgcgc agcgcctcgc ggctggcggc gccgaacagg gcgagggcgc
      661 cgggattgca atcgacgatg gcgtgaccgt cgaggatcag cacgctttcg gcggtcgact
      721 cgaaaaggct gcggtaacgc tcctccgata cctgcagcgc ctcggtgcgc tcggcgacgc
      781 gccgttcgag atcggcgttg gcttcgtgca gcgcatggtc ggcgcggcgc cagcggcggc
      841 tgaagcggaa cgcgaggagc atgccgatca ggcagggaac cagcaccatc aacgccaccg
      901 actggatgac gcgttgcacc tgcggcgcga tcgccgcgct cagcgccctt tcgctgcgcg
      961 ccaggctgag gtgccagccg tgggcgagcg gcacgacgaa acgcaaccag ggttcgccgc
     1021 tggcatcgct gacgcgctcg accccgtcct tgcccgccag cgaatgatcc ggcgcctggc
     1081 ccgtgctggc gccgacgcgc gaactggcga ccttgccgcc ggcggcgtcg aacagcgcgg
     1141 cgtgctcgaa gggcgcgatg cgctcggcaa ccagcaggtc ggccaggcga ctgaggtgaa
     1201 tgacaccgcc cagatgcagc acgatgcgcc ccgaagcgtc gcgcaccggg acgtcgatcg
     1261 ccaccgccgg caccccgtcg gcaccaagga acccgccgga gacgacggct tcgccgctgc
     1321 tcgccgcgtc gcggaaatag cggcgatcgg cgaggttgta cttgcgcagc gcacgctgca
     1381 cgctgaaggg atgcgagatg tagtggtcgc cttccggcgt cagtacgaaa aggacggaga
     1441 aattgccctg gcggcgcagg ctttcgagaa tctcgcgttt ctccgggtcg acgttctcgg
     1501 gaatgccgtt gatgttgcgg tcgatctgcg ccagcgccgg caagtcgctg aaggcgcgcg
     1561 tctgcgcggc gaggcgcagc agttgcagcg gctggtcgat ctcggcatcg aggcgcgccg
     1621 ccaacgcggc gacgaaggcg cggttgtcct cggtccagcg cgccaccgcc cagtcgagca
     1681 acgaggcgcg caggaagaag acgagcaggg ccagcgacag cagcgtcggc agcagcacca
     1741 gggcaacgat gtgtcgccgc tcttccttca tcccggtcgc gccccctgcc aatgattgca
     1801 attttggcaa ttatgcggac aaaccctgcc aaagcaagcc gggaaatccc gcccccgaga
     1861 gtttgccctt acggccccgc cgggtcgctg acgaaaccga tcttgctcaa acccttgcgg
     1921 gccgcgatcg ccatcacctg cgccaccttt tcgtagggca ccaggcgatc
//
LOCUS       DAOSDX010000590         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19794_length_1966_cov_3.304553, whole genome shotgun sequence.
ACCESSION   DAOSDX010000590 DAOSDX010000000
VERSION     DAOSDX010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19794_length_1966_cov_3.304553"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PLN02_11910"
     CDS             complement(<1..1016)
                     /locus_tag="PLN02_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53076.1"
                     /translation="MKPMLRLHLGDQALGAWIVRRGEPHQIGEFPETPSGHDEFSRFL
                     NRQPAAGHLLLLDHAAPRLIAETLPTAGFLANRRMLALRLERLYPDTRWRCAIRLPAP
                     AATSRSIAFLALPETPALQGWLDDLAAASISPRGIHCAPLLLAPFLARRQQLTARLLV
                     LSQHDQAIRLCLLQQGRPAALAQEAHVQPERIPEAIAHFHHHICLSGTTKAPTIPLCL
                     LGTAEWQEQIEVPGKPLRIVTRYADSNIDLLALPAWRWPRRRFPPPALPHPNHRRIAR
                     ALWLAGSVASIGGLALTLATQSTLETRNAEIDRTRARLRSLEQASRELESKARQLGLT
                     TAQMP"
     gene            complement(1013..>1966)
                     /locus_tag="PLN02_11915"
     CDS             complement(1013..>1966)
                     /locus_tag="PLN02_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI53077.1"
                     /translation="DLAAGGSSKPLRHLYRGIADELRSGKSFSAALPGEITRSDASFA
                     GIVRAGELSGRLAETLGRLGSSLRQAVALASATRKLLTYPLLAATLVLTACLFLMFFL
                     VPQIRSFLGNAVTDLPWYTRLLFSLSTLLQEHWAILLATPPLLALAAIIAIRQHPAWH
                     EAWHRLLLHIPLLGEIQRKLLVARLADLLAMLYASGVPLLEALELLPQSIGNRAVARA
                     VTQVHHLVEQGEALSGAFSRHDIFPGLLTRMLETGERTGRLDASLNNLAGIYEQEARA
                     TIGTLQALIEPALTLSIGILLGWMMLATLQPIYDIVEQVAR"
BASE COUNT          334 a          537 c          764 g          331 t
ORIGIN      
        1 ggcatctgcg cagttgtcag gccaagctgc ctggccttgc tctccagttc ccggcttgcc
       61 tgttcgagcg accgcaggcg ggcgcgggta cggtcgattt cggcattccg cgtttccagc
      121 gtcgattgcg tggccagcgt aagcgccagg ccaccgatgc tggcgacact accggccagc
      181 cacagggcgc gagcgattcg ccggtggttt gggtgcggca acgcgggcgg tggaaaccgg
      241 cggcgcggcc agcgccaggc tggcagggcc agcaggtcaa tgttgctgtc ggcgtatcgg
      301 gtgacgatgc gtagcggttt gcccgggact tcgatctgct cctgccattc ggcggtgccg
      361 agcaggcaga gggggatggt gggggctttt gtcgtgccgg aaaggcaaat gtggtggtgg
      421 aagtgggcaa tcgcttccgg gatgcgttcg ggttgcacat gcgcttcctg agcgagtgcg
      481 gcgggtcggc cttgctgcag gaggcagagc cggatggcct ggtcgtgctg gctgagcacc
      541 agcaggcggg ctgtcagttg ctgacggcgg gcaaggaacg gcgccagcag cagtggcgcg
      601 caatggattc cccgtggact gatcgacgcc gcggcgaggt cgtccagcca gccttgcagg
      661 gcaggggttt ccggcaaggc gaggaaggcg atcgagcggc tggtagcggc aggcgccggc
      721 aagcgaatgg cacaacgcca acgggtgtcg gggtaaagac gttcgaggcg cagcgcgagc
      781 atccggcgat tggcgaggaa acccgccgtc gggagggttt cggcaatcag gcgtggggcg
      841 gcgtggtcga gcagcaggag atggccggcc gccggttgcc ggtttagaaa acgggaaaat
      901 tcgtcatgcc cggatggcgt ttccgggaac tcgccgatct ggtgtggttc gcctcgccgc
      961 acgatccagg cgccaagcgc ctgatcgccg aggtggaggc ggagcattgg cttcaccgtg
     1021 ccacctgttc gacgatgtcg taaatcggct gcagggtggc cagcatcatc cagccgagga
     1081 ggatgccgat gctcagggtc agggcgggtt cgatcagcgc ctgcagggtg ccgatggtgg
     1141 cgcgggcttc ctgctcgtag atgccggcga gattgttcag cgaggcgtcg aggcggcctg
     1201 tgcgttcgcc ggtttcgagc atccgggtca acaggccggg gaaaatgtcg tgtcgggaaa
     1261 aggcgccgga caatgcttcg ccctgttcga ccagatgatg gacctgtgtc accgcccggg
     1321 cgacagcgcg attgccgatc gactgaggca gcagttcgag ggcttcgagg aggggaacgc
     1381 cgctggcgta gagcatggcc agcagatcgg cgaggcgggc gaccagcagt ttgcgctgga
     1441 tttcgccgag cagggggatg tgcagcaaca gtcggtgcca ggcttcgtgc caggcagggt
     1501 gctgccggat ggcgatgatg gcggcgagtg cgagcagcgg cggcgttgcc aggaggattg
     1561 cccagtgctc ctgcagcagt gtcgacagcg agaacagcaa gcgggtgtac cacggcagat
     1621 cggtcactgc attaccgagg aaactgcgaa tctgtggcac caggaagaac atcaggaaga
     1681 ggcaggccgt gagaacgagc gttgcggcga gcaaggggta ggtgaggagc ttgcgagtgg
     1741 cgctggccag ggccactgcc tggcgcaggc tgctgccgag gcggccgagg gtttcggcga
     1801 gccggccgct gagttcgccg gcgcggacga taccggcgaa actcgcgtcg ctgcgggtga
     1861 tttctcctgg cagtgcggca gaaaaggatt tgccgctgcg tagttcatcg gcaatcccgc
     1921 ggtagagatg gcgcagtggc ttgcttgaac cacccgcggc caggtc
//
LOCUS       DAOSDX010000591         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19844_length_1963_cov_2.949686, whole genome shotgun sequence.
ACCESSION   DAOSDX010000591 DAOSDX010000000
VERSION     DAOSDX010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19844_length_1963_cov_2.949686"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /gene="pilW"
                     /locus_tag="PLN02_11920"
     CDS             <1..768
                     /gene="pilW"
                     /locus_tag="PLN02_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HOI53078.1"
                     /translation="AFAQFDGALPAAAQQPSPQLRQDSQPLTVADPRTRAKIHTELGA
                     LYFQGGNPAAAIDHLGQALEIDANYFQALSVRGLVYASLREFAKADRDFAEALARAPS
                     DPEVNNNYGWFLCETGKQREAIAYFDRALRDPLYDTPDLALTNAGNCLVKLGDADQAM
                     NYYLQALRVSRSGAVGARIGLAGLIHKRGNSAEARVYLNEALRMMEPPTAEALWLGIR
                     IERKLGDRAAESGFAAQLRSRYPTSNEYQEFLKGNFE"
     gene            765..1679
                     /locus_tag="PLN02_11925"
     CDS             765..1679
                     /locus_tag="PLN02_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI53079.1"
                     /translation="MSEQIENSSMPEVDEPTQTMAFALSVGNQLRQAREARGLGVGDI
                     AQALKLGPRQVEALERDEWTDLPGTTFIRGFVRNYARLVGVDPQPLMAHLGNLLEKPV
                     DTLNVPAAAPTTMRSGNRSRDRAVVAIGVGLVVVAVLAYFLLPSDLAAWRANLQSLLD
                     ASPTSAETSEAAAAQEPLFPPPEAAAPAAVLAPTIEVPATVAPAALVNPLPVQPTPPV
                     QPVANSTTDGQLRFHVARESWVEVRDRDDVVVFSQRLPAGSEQQVGGKAPLSVTIGYA
                     PGVSLMAHGKVVDLAPHTRGDVARLLVE"
     gene            1683..>1963
                     /locus_tag="PLN02_11930"
     CDS             1683..>1963
                     /locus_tag="PLN02_11930"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOI53080.1"
                     /translation="MTALAKRPTRATTIAHVRLGGDAPVVIQSMTNTDTADYLSTAIQ
                     CAELARAGSELVRITVNSPEAAAAVPKIRDHLDKMNCNVPLIGDFHYNG"
BASE COUNT          336 a          640 c          641 g          346 t
ORIGIN      
        1 gcattcgccc agttcgatgg cgcactgcct gccgctgccc agcagccgtc accgcagctc
       61 aggcaggaca gccagccgct gacggtggct gacccgcgga cgcgggccaa gattcatacc
      121 gaactggggg cgctctactt ccagggcggc aatccggcag ccgccatcga tcatctcggg
      181 caggccctgg aaatcgacgc taactacttc caggcgctga gcgtgcgtgg cttggtgtat
      241 gcatcgctgc gtgaattcgc caaggccgac cgcgatttcg ccgaagccct ggcgcgggcg
      301 cccagcgacc ccgaagtcaa taacaactat ggctggttcc tttgcgaaac cggcaagcag
      361 cgcgaagcga ttgcctattt cgaccgcgcc ctgcgcgatc cgctctacga tacgcccgat
      421 ctggcgctga ccaacgccgg caactgcctg gtcaagctgg gcgatgccga tcaggcgatg
      481 aactactacc tgcaggcgct acgggtttcg cgcagcggcg ccgtcggcgc ccggatcggt
      541 ctggccggcc tgattcacaa gcggggcaat tcggccgagg cgcgtgtcta tctgaacgag
      601 gccctgcgca tgatggagcc gccgaccgcc gaagcgctct ggttgggcat ccggatcgag
      661 cgcaagctcg gcgaccgggc ggccgaaagc ggttttgccg cccagttgcg cagccgttat
      721 ccgacttcga atgaatatca ggaattcctc aagggcaact ttgaatgagt gagcagattg
      781 aaaactcgtc catgccggaa gtggacgagc cgacgcaaac gatggcgttt gcgttgtccg
      841 tcggcaacca gttgcgccag gccagagagg cgcgcggcct tggcgtgggt gacatcgccc
      901 aggcgctgaa actcggtccg cgccaggtcg aggcgctgga gcgcgacgaa tggacggatc
      961 taccgggaac caccttcatc cgtggtttcg tgcgcaacta cgcgcgtctg gttggtgtcg
     1021 atccgcagcc cctgatggcg catcttggta acttgctgga aaagccggtc gatacgctga
     1081 acgttcctgc cgcggcgccg acgacgatgc gcagcggcaa ccgttcgcgc gatcgggcgg
     1141 tggtcgccat tggcgttggt ctggtggtgg ttgcggtgct ggcctatttc ctgttgccca
     1201 gcgatcttgc cgcctggcgt gccaacctgc agtccttgct cgatgccagc ccgacgagcg
     1261 cggagaccag cgaagccgcg gcggcgcagg agccgctttt cccgccgccc gaggcggctg
     1321 caccagcagc agtgcttgcg ccgacgatcg aagttccggc gacggtggcg ccagccgccc
     1381 ttgtcaaccc gttaccggtt cagccgacgc cgccggttca gccggttgcc aacagcacaa
     1441 cggacgggca actgcgtttc cacgtcgctc gcgagtcctg ggttgaagta cgtgaccgcg
     1501 acgatgtggt ggtgttttcc cagcgtctgc cggcgggtag cgaacagcag gtcggcggca
     1561 aggcgccgct gtcggtgacc atcggctatg cgcccggggt cagcctgatg gcgcatggca
     1621 aggtcgtcga tctggcgccg catacccgtg gcgatgtcgc ccgtctgctg gtggagtaag
     1681 ccgtgactgc ccttgccaag cgcccgaccc gcgccaccac catcgctcac gttcgcctcg
     1741 gtggcgacgc gccggtggtc atccagtcga tgaccaacac cgacactgcc gattacctgt
     1801 ccaccgccat ccagtgcgcc gagctggcgc gcgccggttc cgagctggta cggatcacgg
     1861 tcaattcgcc ggaagcggcg gcggcggtgc cgaagatccg cgatcatctc gacaagatga
     1921 actgcaatgt gccgctgatc ggtgatttcc actacaacgg cca
//
LOCUS       DAOSDX010000592         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19864_length_1962_cov_2.242790, whole genome shotgun sequence.
ACCESSION   DAOSDX010000592 DAOSDX010000000
VERSION     DAOSDX010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19864_length_1962_cov_2.242790"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="PLN02_11935"
     CDS             complement(<1..1716)
                     /locus_tag="PLN02_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HOI53081.1"
                     /translation="MIPELGHFALILAALVALLLGTLPLIGAHNGNQPWTAICRPAAG
                     AFSLLVTVSFACLTWAFVANDFSVAYVAQHSNSLLPLPYRIAAVWGGHEGSLLLWMQM
                     LACWLLAVRLFSRQLPEVMLARVLGSLGLVSFGFLLFILITSNPFERLLPGVAEGRDL
                     NPLLQDPGMVIHPPLLYMGYVGFSVAYAFAIAALLSGKLDAAWARWSRPWTTGAWIFL
                     TLGIAMGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGSFKNWTV
                     LLAISAFSLSLLGTFLVRSGVLTSVHAFATDPTRGVFILVFLVAVIGTSLALFAWRAP
                     SVGLGGRFGLISRESLLLTNNVLLVVACATVLLGTLYPLLIDALGIGKISVGPPYFDT
                     VFFPVMMPVLFLMGIGPFARWKEASLGEIARLLRWAFAVAVVVAIAVPLLYGQWKVLT
                     ALGLLLAAWIVVTALIHFVRRVNQTRGGRGFFAAALDQPASFVGMHVAHIGIAVFVVG
                     VSMVSTYEEEKDVKMGPGDVVDVGGYQFRFNGVQSLQGPNYQAAQGDFDLLLGGKLER
                     KLFPEK"
     gene            complement(1719..>1962)
                     /locus_tag="PLN02_11940"
     CDS             complement(1719..>1962)
                     /locus_tag="PLN02_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOI53082.1"
                     /translation="IVVDYVGILPDLFVEGKGAVAQGRLLPDGTFKAQEVLAKHDENY
                     MPPEAAKALNDAHERGVQKAAAQGQPAATAPQPASN"
BASE COUNT          428 a          627 c          657 g          250 t
ORIGIN      
        1 tttttccggg aacagcttgc gctccagctt gccgcccagg agcaggtcga aatcgccctg
       61 cgccgcctgg tagttcggcc cctgcaaaga ctggacgccg ttgaaacgga actggtagcc
      121 gccaacatcg acgacatccc ccggccccat cttgacgtcc ttttcctctt cataggtact
      181 gaccatgctg acgccgacga caaaaacggc gataccgata tgcgcgacat gcatgccgac
      241 gaagctcgcc ggctgatcca gcgcggcggc gaagaagccg cggccgccgc gggtctggtt
      301 gacgcgacgg acgaagtgga tcagcgccgt gacgacgatc cacgccgcca gcagcagacc
      361 gagcgcggtc agcaccttcc actggccata gagcagcggc acggcgatcg ccaccacgac
      421 ggcgacggca aaagcccagc gcaggaggcg cgcgatctcg cccagcgacg cttccttcca
      481 gcgggcgaag gggccgatcc ccatcaggaa cagcaccggc atcatgaccg ggaagaagac
      541 cgtgtcgaaa tacggcggcc cgaccgagat cttgccgatg ccgagggcgt cgatcagcag
      601 cgggtagagc gtgcccagca ggacggtggc gcaggcgacg accagcagca cgttgttggt
      661 gagcagcagc gactcccggg aaatcaggcc gaaacgaccg cccagaccga ccgacggcgc
      721 gcgccaggcg aacagggcga gcgaagtgcc gatcacggcg accaggaaga ccaggatgaa
      781 gacgccgcgc gtcggatcgg tggcgaaggc atgcaccgag gtcaggacgc cggaacggac
      841 caggaaggtg ccgagcagcg acagcgagaa cgccgaaatc gccagcagca cggtccaatt
      901 cttgaaactg ccgcgttttt cggtgactgc cagagagtgg atcagcgccg tgccgaccag
      961 ccagggcatg aaggaagcgt tttcgaccgg atcccagaac caccagccgc cccagcccag
     1021 ttcgtaatag gcccaccacg agcccatggc gatgccgagg gtcaggaaga tccacgcccc
     1081 cgtcgtccac ggccgcgacc agcgcgccca ggcggcatcg agcttgccgg agaggagggc
     1141 ggcgatggcg aaggcgtagg ccaccgagaa accgacgtaa cccatgtaga gcagcggcgg
     1201 gtggatgacc atgcccggat cctggagcag cggattcagg tcgcgcccct cggcaacgcc
     1261 aggtaacagg cgttcgaagg gattcgaggt gatcaggatg aagagcagga aaccgaagga
     1321 caccagcccc agcgagccga gcacgcgggc cagcatcacc tccggcaatt gccgcgagaa
     1381 aaggcgtacc gccagcagcc agcaggcgag catctgcatc cacaacagca acgagccctc
     1441 gtggccgccc cacactgcgg cgatgcgata gggaagcggt agcagggaat tggaatgctg
     1501 ggcgacatag gcaacggaaa aatcgttggc gacgaaagcc caggtcaggc aggcaaagga
     1561 aaccgtgacc agcagggaga aggcgccggc cgccggccgg cagatggcgg tccagggttg
     1621 attgccgtta tgggcgccaa tcaggggaag ggtgcccagc aggagcgcaa ccagcgccgc
     1681 caggatcagg gcgaaatggc caagttcagg aatcatgctc agttactcgc gggttgcggt
     1741 gccgtcgccg ccggttgtcc ctgcgcggcg gccttctgga cgccgcgctc gtgcgcgtcg
     1801 ttgagcgcct tggccgcttc cggcggcatg tagttctcgt cgtgcttggc cagaacttcc
     1861 tgcgccttga acgtgccgtc cggcagcagg cgcccctggg caaccgcccc cttgccttcc
     1921 acgaagaggt cgggaaggat gccgacgtag tcgacgacga ta
//
LOCUS       DAOSDX010000593         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19918_length_1958_cov_3.184446, whole genome shotgun sequence.
ACCESSION   DAOSDX010000593 DAOSDX010000000
VERSION     DAOSDX010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19918_length_1958_cov_3.184446"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLN02_11945"
     CDS             <1..647
                     /locus_tag="PLN02_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOI53083.1"
                     /translation="EYLSRNDLEVSIEPRGDTAEARILAEQPDLVILDVMLPGKDGFE
                     VCRGVRQQYRGVILMLTARDEDFDQILGLELGADDYIAKPVQPRVLLARIKALLRRLP
                     VSGDDAGDSDSQVFGQFRISQATRTATLNAQPIDLTTAEFDLLWLLASHAGNVLSRDD
                     LLQELRGIGFDGLDRSIDARISRLRKKLNDDPENPTRIKTVRGKGYLFSKHDWN"
     gene            634..>1958
                     /locus_tag="PLN02_11950"
     CDS             634..>1958
                     /locus_tag="PLN02_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI53084.1"
                     /translation="MTGTEGEAGAPSGKPGRQGLARSLFRVSLPRWRGSRYSLSKLFL
                     NFYLLAMGSFVAIAFIADFIISTAQQGITDDYARRFMRGTITLIEDELFRHPRRQWER
                     KIREIDGKFSYKLDIVERRSLDIRLTSAQVEKLDAGDIAIAHDHDVMYHRLGTSSKVL
                     VVGPLSATHNPEHNERLPLDLRLRLLTWSLIGVIFAIALWFWVRPVWRDLESLRQTAR
                     DLGDGDFEAPPPVVHTQLFAPLADTLISMSERVRQLLATHRELSCGISHELRTPIARM
                     RFALEMLSETTEIGERQRLWAMMEADLDELDQLIDTSLTYARFEREAPEAHFSSVAFA
                     DWLSDEVDSVRLLGRQLNVSVDTSLLPENLRVDLDRKAMPYALRNLLRNAFKYAAREI
                     RVSSELLGDQIAVHVDDDGIGIPEGEREHIFSAFTRLDRSRDRSTGGYGL"
BASE COUNT          373 a          691 c          539 g          355 t
ORIGIN      
        1 ccgaatacct gtcccgcaac gatctcgaag tcagcatcga gccccgcggc gacaccgccg
       61 aggctcgcat actcgccgaa cagcccgatc tcgtcatcct tgacgtgatg ctgcccggca
      121 aggatgggtt cgaagtctgt cgcggcgtcc gccagcaata ccgtggcgtc atcctgatgc
      181 ttaccgcccg tgacgaggac ttcgaccaga ttcttggtct cgaactcggc gctgacgact
      241 acatcgccaa accggtacag ccgcgcgtcc tgctggcgcg gatcaaggcc ttgctgcgcc
      301 gcctgccggt cagcggcgac gatgccggcg acagcgactc gcaggtcttc ggccagttcc
      361 gcatcagtca ggcaacccgc accgccaccc tcaacgctca accgatcgac ctgaccactg
      421 ccgagttcga cctgctctgg ctgctcgcca gccatgccgg caacgtgctg tcgcgcgacg
      481 acctgctgca ggagttgcgc ggcatcggct tcgacggcct cgaccgctcg atcgacgccc
      541 gcatctcccg actgcgcaag aagctgaacg acgatccaga gaacccgacg cgcatcaaga
      601 ccgtacgcgg caagggctac ctgttcagca agcatgactg gaactgaggg agaagccggc
      661 gcgccctccg gcaagccagg ccgccaggga ctggcccgct ccttgttccg ggtatctttg
      721 ccacgctggc gaggcagccg ctacagcctg tccaagctct tcctcaattt ctacctgctg
      781 gcgatgggct ccttcgtcgc catcgccttc atcgccgatt tcatcatctc gacggcgcaa
      841 caaggcatca ccgacgacta cgcccgccgc ttcatgcgcg gcaccatcac cctgatcgaa
      901 gatgaactct tccgccatcc ccgacgccag tgggaacgca agatccggga aatcgacgga
      961 aagttttcct acaagctcga tatcgtcgaa cgccgctcgc tcgacatccg cctgaccagc
     1021 gcccaggtcg agaaactcga tgccggcgac atcgccatcg cccacgacca cgacgtgatg
     1081 tatcaccgcc tcggcaccag cagcaaggtg ctggtcgtcg gcccgctatc ggccacccac
     1141 aacccggaac acaacgaacg cctgccgctc gatctgcggc tgcggctact gacctggagc
     1201 ctgattggcg tcattttcgc gatcgccctc tggttctggg tgcgccccgt ctggcgcgat
     1261 ctcgaatcgc tgcgccagac cgcccgcgac ctcggtgacg gcgacttcga agcgccgccg
     1321 ccggtggtgc atacgcaact gttcgcgccg ctcgccgata cattgatcag catgtcggag
     1381 cgggttcgcc aactgctggc aacgcaccgt gaactctcct gtggcatttc gcacgaactg
     1441 cgcacgccga ttgcccgcat gcgcttcgcc ctcgaaatgc tttcggaaac caccgagatc
     1501 ggcgaacgcc aacgcctttg ggcgatgatg gaagccgacc tcgacgaact ggatcaattg
     1561 atcgatacca gcctgaccta cgctcgtttc gaacgagaag ccccggaagc ccatttttca
     1621 agcgtcgctt tcgccgactg gctgagcgac gaggtcgact cggtcaggct gctcggtcgc
     1681 cagttgaatg tcagcgtcga taccagcctg ctacctgaaa accttcgggt tgatctcgac
     1741 cgcaaggcaa tgccctacgc gctacgcaac ctgctgcgca atgccttcaa gtatgccgcg
     1801 cgtgaaatcc gggtcagcag cgaactcctc ggcgaccaga ttgccgttca tgtcgacgac
     1861 gacggcatcg gcatcccgga aggcgaacgc gaacatatct tttccgcctt cacccgtctt
     1921 gatcgctcac gcgaccgttc gaccggtggc tatggtct
//
LOCUS       DAOSDX010000594         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19959_length_1954_cov_2.876251, whole genome shotgun sequence.
ACCESSION   DAOSDX010000594 DAOSDX010000000
VERSION     DAOSDX010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19959_length_1954_cov_2.876251"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLN02_11955"
     CDS             <1..306
                     /locus_tag="PLN02_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOI53085.1"
                     /translation="DFFEKPFNDNELVSRIAEAIDLDARQRAAHAEADSVNSRISRLT
                     TRERQIMDLVLAGKYNKVIADELNISMRTVEVHRANLFDKMQVKTAVELANLFKNGR"
     gene            433..759
                     /gene="trxA"
                     /locus_tag="PLN02_11960"
     CDS             433..759
                     /gene="trxA"
                     /locus_tag="PLN02_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HOI53086.1"
                     /translation="MSEHIHYVTDDTFESEVLQAQQPVLVDYWAEWCGPCKMIAPILD
                     EIAVEYSGKLKVAKVNIDDNQQTPAKYGIRGIPTLMIFKNGNVEATKVGALSKSQLAA
                     FIDSNL"
     gene            918..>1954
                     /gene="rho"
                     /locus_tag="PLN02_11965"
     CDS             918..>1954
                     /gene="rho"
                     /locus_tag="PLN02_11965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOI53087.1"
                     /translation="MQLSELKTLHVSKLLDMATELAIENANRMRKQELIYAVLKAKAK
                     NGETIYGDGTLEVLPDGYGFLRSADTSYLANPDDIYVSPSQVRRFNLRTGDTVEGEIR
                     TPKDGERYVALTKLDRINGFPPEANKNKIMFENLTPLHPTRHLKLEREIKSDENITSR
                     VIDMIAPIGCGQRGLLVAPPKTGKTVMLQNIAHAITANHPEVVLIVLLIDERPEEVTE
                     MTRTVRGEVVASTFDEPATRHVAVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VQPASGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTILATALIDTGSRMDEVIYEE
                     FKGTGNSEIHL"
BASE COUNT          467 a          698 c          496 g          293 t
ORIGIN      
        1 gacttcttcg aaaaaccctt caacgacaat gaactggtca gccggatcgc cgaagcgatc
       61 gacctcgacg cccgccagcg cgcggcgcac gccgaagccg actcggtcaa cagccggatt
      121 tcgcggctca ccacccgcga acgccagatc atggacctgg tgctggccgg aaaatacaac
      181 aaggtgatcg ccgacgaact caacatcagc atgcgcaccg tcgaagtgca ccgcgccaat
      241 ctgttcgaca agatgcaggt caagaccgcc gtcgaactcg ccaacctgtt caagaacggg
      301 cgttgataca agcttgcaaa tgcacaggcg gatgcttccc ttgcctgtca tttttcgcta
      361 gcatcacgcc agactgaatt gcggcagacg ccgccccatc cgaacaccac tcccagaatc
      421 cacgaggcaa ccatgagcga gcacatccat tacgtcaccg acgacacctt tgaaagcgaa
      481 gtgctgcagg cacagcaacc ggtgctcgtc gactactggg cagaatggtg cggcccctgc
      541 aagatgatcg cgccgatcct cgacgaaatc gccgtcgaat acagcggcaa gctgaaagtc
      601 gccaaggtca acatcgacga caaccagcag acgccggcca agtacggcat ccgcggcatc
      661 ccgacgctga tgatcttcaa gaacggcaat gtcgaggcaa ccaaggtcgg cgccctctcc
      721 aagtcgcaac tcgctgcctt tattgacagc aacctgtaat acccttagtc tcccggcctg
      781 aaggtacgct cgtgccttca ggcgacgcca gcgacgttcc cgtcccgcgt catacatcac
      841 tcccaagcac actcagcttc cgcgcccttt catcgggctg acgcctgcgc tcattctcca
      901 gcgcccaata cactccaatg caactttccg aactcaagac cctgcacgtc agcaaactgc
      961 tcgacatggc gaccgaactc gccatcgaaa acgccaaccg catgcgcaag caggagctga
     1021 tctacgccgt gctcaaggcc aaggccaaga acggcgaaac catctacggc gacggcacgc
     1081 tcgaagtcct gcccgacggc tatggcttcc tccgctcggc cgacacctcc tacctggcca
     1141 accccgatga catctacgtc tcgccgtcac aggtgcgccg cttcaacctg cgcaccggcg
     1201 acaccgtcga aggcgaaatc cgcacgccga aggatggcga acgctacgtc gcgctgacca
     1261 agctcgaccg catcaacggc ttcccgccgg aagccaacaa gaacaagatc atgttcgaga
     1321 acctgacgcc gctgcacccg acgcgtcacc tcaagctcga acgcgaaatc aagtccgacg
     1381 aaaacatcac cagccgcgtc atcgacatga tcgccccgat cggctgcggc cagcgcggcc
     1441 tgctcgtcgc cccgccgaag accggcaaga ccgtgatgct gcagaacatc gcgcacgcga
     1501 tcaccgccaa ccacccggaa gtcgtactga tcgtcctgct gatcgacgaa cgcccggaag
     1561 aagtcaccga aatgacccgc accgtcaggg gcgaagtcgt cgcctcgacc ttcgacgaac
     1621 cggccacccg ccatgtcgcc gtcgccgaaa tggtcatcga gaaagccaag cgtctggtcg
     1681 agcacaagaa ggacgtggtc atcctgctcg actcgatcac ccgcctcgcc cgcgcctaca
     1741 acaccgtaca gcccgcctcc ggcaaggtgc tgaccggcgg cgtcgacgcc aacgccctgc
     1801 aaaaacccaa gcgcttcttc ggcgccgcgc gcaacataga ggaaggcggc tcgctgacca
     1861 tccttgccac cgcgctgatc gacaccggct cgcgcatgga cgaagtgatc tacgaagaat
     1921 tcaagggcac cggcaactcg gaaatccacc tcga
//
LOCUS       DAOSDX010000595         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19960_length_1954_cov_2.780411, whole genome shotgun sequence.
ACCESSION   DAOSDX010000595 DAOSDX010000000
VERSION     DAOSDX010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19960_length_1954_cov_2.780411"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLN02_11970"
     CDS             <1..637
                     /locus_tag="PLN02_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53088.1"
                     /translation="GGHRSNAGQPAGAKRALAQPRHAGCCQHSPRLRQLLATPDGGSE
                     VKHWLAIAFVYRQPLLFCGGALLAGATAVLYAEQQLDAASSRLAALQQQAAQTTQRLA
                     ELRGAAAAFDGLALAATAPNDLLAGIASAQDLAIETRPQRETLAGDWRTSEFRLHGPL
                     LHEEILLDIVDRWSRLSAFQHQLHHCALHRQAARLFADCQFVQLLPSGSTR"
     gene            634..945
                     /locus_tag="PLN02_11975"
     CDS             634..945
                     /locus_tag="PLN02_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53089.1"
                     /translation="MIRLLCLLLLGATLNCRAEPLGRLFFTPEQRRLLEQAPAAAPAL
                     APGRLDGIVVGSTGLNLRWVDGQLDTDANSGRKVGDSMRQPLLPPGNLHVGTAAEEGM
                     K"
     gene            942..>1954
                     /locus_tag="PLN02_11980"
     CDS             942..>1954
                     /locus_tag="PLN02_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53090.1"
                     /translation="MRHQSGFALFAPLLLGAFAAAGWLQHKPPPRQAEQARQARALAA
                     AREALIARAANDANRPGSLPCPDQSTDSTGFANHPGDGKADMLVGNHCPSYLGRLPWI
                     TLDMPPPRDRAQEILWYVLAPGLRDDDSAEPINSSTATELIHAGHSEVAALLIAAGPP
                     LARQERPSHTPGDYLEGWLENSPTRTYWANTSPSNDQILIIHRDEVMAMVMRRVAQSL
                     RHCLQAHAASSGRFPWPSPFAAANGDGQQGARFGRIPLSQPEDSAEKRLAEIRAHYQE
                     SIAALAAAGDPAHRQEALNRLATASAWSANWLRSQRLVGETFKRLSDATTTPLADLAQ
                     KIA"
BASE COUNT          345 a          737 c          601 g          271 t
ORIGIN      
        1 cggcggacac cgcagcaacg ccggccagcc tgcaggggct aagcgagcgc ttgcgcagcc
       61 gcggcatgcg ggctgttgtc agcacagccc ccggctccgc caacttctcg ctacgcctga
      121 cggcggatcg gaagtgaagc actggctcgc catcgccttc gtctaccgcc agccgctgct
      181 cttctgcggc ggggccttgc tggccggcgc gacggccgtg ctctacgccg aacaacagct
      241 tgacgccgcc agcagcagac tcgccgcact ccagcagcag gccgcccaaa cgacgcaacg
      301 cctcgccgaa ttgcgcggtg ccgccgccgc attcgacggg ctggcgctcg ctgctacggc
      361 accgaatgac cttttggccg gtatcgcatc agcccaggac cttgcaatcg aaacccgccc
      421 ccaacgagaa actttagccg gagactggcg caccagcgaa tttcgcctgc acggcccact
      481 gttgcacgaa gaaatcctgc tcgacatcgt cgatcgctgg agccggctgt cggcgttcca
      541 gcaccagctt catcactgcg cgctccaccg gcaagcggca cgactttttg ccgattgcca
      601 gttcgtgcag ctcctgccga gcggctcgac ccgatgatca ggctgctttg cctgctcctg
      661 ctgggggcca ccctgaactg ccgggccgag ccactgggac ggctgttctt cacccccgaa
      721 caacgccgtc tgctggagca ggccccggca gcagcgccag cactggcgcc cggccgtctc
      781 gacggcatcg tcgtcggcag cacgggcctg aatctgcgct gggtcgacgg ccaactcgac
      841 accgacgcaa acagcggccg gaaggtcggc gacagcatgc ggcagcccct gctgccaccc
      901 ggcaacctgc atgtcggcac cgccgcggag gaaggcatga aatgagacat cagtccggct
      961 tcgcgctgtt cgccccactg ctgctgggcg ctttcgccgc agcgggctgg ctgcagcaca
     1021 aaccgccgcc gcgacaggcc gagcaagccc gccaggcccg tgccctggct gccgcgcgcg
     1081 aggccctgat cgcccgcgcc gcgaacgacg ccaaccgtcc cggcagcctc ccctgccccg
     1141 accagagcac cgacagtacc ggctttgcca accaccctgg cgatggcaag gccgacatgc
     1201 tggtcggcaa tcactgcccg agctatcttg ggcgcctgcc ctggatcacg ctggacatgc
     1261 cgcccccccg cgaccgggcg caggagatcc tctggtacgt gctcgccccg ggcctgcgcg
     1321 acgacgacag cgccgaaccg atcaacagca gcactgccac cgaactgatc catgccgggc
     1381 acagcgaggt cgccgccctg ctcatcgcag cggggccacc gctggcgcgg caggagcggc
     1441 cttcgcacac gccgggtgat tacctcgaag gctggctcga aaactcgccg acgcgaacct
     1501 attgggctaa caccagcccg agcaacgatc agattttgat catccaccga gacgaagtga
     1561 tggcaatggt catgcgccgg gtcgcccagt cgcttcgcca ctgcctgcaa gcccacgctg
     1621 ccagcagcgg gcgctttccc tggccgtccc cgtttgccgc ggcgaacggg gacggccagc
     1681 agggtgcgcg cttcggacgt atcccgctga gccagccgga ggacagtgcg gaaaaacgcc
     1741 tggccgagat tcgggcgcac tatcaggaaa gcatcgccgc gcttgccgcg gccggcgacc
     1801 cggcacaccg ccaggaagca ctgaaccgcc tggcgacagc gagcgcctgg agcgccaact
     1861 ggctgcgcag ccagcgcctg gtcggcgaaa cgttcaagcg cctgagcgac gctaccacca
     1921 ccccgctcgc ggacctcgcc cagaaaatcg ccaa
//
LOCUS       DAOSDX010000596         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19986_length_1952_cov_2.694781, whole genome shotgun sequence.
ACCESSION   DAOSDX010000596 DAOSDX010000000
VERSION     DAOSDX010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19986_length_1952_cov_2.694781"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLN02_11985"
     CDS             <1..653
                     /locus_tag="PLN02_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOI53091.1"
                     /translation="TAVSIVFRWLEWVGEDAGKKYHWRMIPASNPDGLLIRPSTRVNS
                     KGVDLNRNFPTPDWDSDAKKYWIERTGRDPRRFPGESGGSEIETRWLEKQIADYKPDV
                     IISIHAPYNLLDYDGPAPQPLRFGRLALNRLGVYPGSLGNYGGLHKNVPVVTIELPNA
                     TMMPSPRDQRAMWVDMLKWMDRNIKAGNGSAKETTKRPEKYGEPVFKGNIDHKKID"
     gene            complement(660..1928)
                     /locus_tag="PLN02_11990"
     CDS             complement(660..1928)
                     /locus_tag="PLN02_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOI53092.1"
                     /translation="MAEQLRPQTPDEVIGQQHLLGPGKPLRLAFASGQPHSMILWGPP
                     GVGKTTLARMMATQFQCEFIALSAVFSGIKEVREAVAQAELWKAQGKRTILFVDEIHR
                     FNKSQQDGFLPFVESGLFTFIGATTENPSFEVNSALLSRASVYVLKSLDEAEMGQLFD
                     RAQARALADLAFDEEAKTRLIGLADGDARRLLNLLEQVRTAARAAGGSAVDGAFIEAT
                     MAQNLRRFDKGGDAFYDQISALHKSVRGSSPDGALYWFCRMLDGGADPRYLARRIIRM
                     AWEDIGLADPRAMQIANDAAETYERLGSPEGELALAQAVTYLAVAAKSNAGYNAYNAA
                     RAFVAKDGSRPVPVHLRNAPTKLMKELGYGKAYRYAHDEPEAYAAGESYLPDGMPEPG
                     WYAPTPRGLEGKIGEKLAHLRDLDRKAGKK"
BASE COUNT          353 a          686 c          603 g          310 t
ORIGIN      
        1 tgaccgccgt ctccatcgtt ttccgctggc tcgaatgggt cggcgaggac gccggcaaga
       61 aatatcactg gcggatgatt ccggcgtcca atccggacgg cctgctgatc cgcccatcga
      121 cccgggtcaa cagcaagggc gtcgatctca accgcaactt cccgaccccg gactgggaca
      181 gcgacgccaa gaaatactgg atcgaacgca ccggccgcga cccgcggcgc tttcccgggg
      241 aatccggcgg ctcggaaatc gagacgcgct ggctggaaaa gcagatcgcc gactacaagc
      301 cggacgtcat catcagcatc cacgcccctt acaacctgct cgactacgac ggcccggcgc
      361 cgcaaccgct gcgcttcggc cgtctggcgc tcaaccgtct cggcgtctac ccgggttcgc
      421 tgggcaatta cggcggcctg cacaagaacg tgccagtggt caccatcgaa ctgccgaacg
      481 cgacgatgat gccgtcgccg cgcgaccagc gggcgatgtg ggtggacatg ctgaaatgga
      541 tggaccgcaa catcaaggcc ggcaacggca gcgccaagga aaccaccaag cgcccggaaa
      601 aatatggcga gcccgtcttc aagggcaaca tcgatcacaa gaagatcgac tgaccccagc
      661 tacttcttgc ccgccttgcg atccagatcg cgcaggtggg cgagtttttc accgatcttg
      721 ccctccagcc cgcgcggcgt cggcgcgtac cagccgggct caggcatgcc gtccggcagg
      781 tagctctcgc cggcggcata ggcttccggc tcgtcgtgcg catagcggta agccttgccg
      841 taaccgagtt ccttcatcag cttggtcggc gcgttgcgca ggtgcaccgg caccggccgc
      901 gaaccgtcct tggcgacgaa ggcgcgcgcc gcgttgtagg cgttgtagcc ggcgttcgac
      961 ttggccgcca ccgccaggta agtcaccgcc tgcgccaggg ccagctcgcc ttccggcgaa
     1021 ccgaggcgct cgtaggtttc ggccgcgtcg ttggcaatct gcatcgcccg cgggtcggcc
     1081 aggccgatgt cttcccaggc catgcggatg atccgccgcg ccaggtaacg cgggtcggcg
     1141 ccgccgtcga gcatgcggca gaaccagtag agcgcgccgt ccgggctgga accacgcacc
     1201 gacttgtgca gcgccgaaat ctggtcgtag aaggcgtcgc cgcccttgtc gaaacgacgc
     1261 aggttctgcg ccatggtggc ctcgatgaag gcgccgtcga ccgcggaacc ccccgccgcc
     1321 cgcgccgccg tgcgcacctg ttcgagcagg ttgagcaggc gccgcgcgtc gccgtcggcc
     1381 aacccgatca gccgcgtctt cgcttcctcg tcaaacgcca gatcggccag cgcccgcgcc
     1441 tgggcgcggt cgaacaattg ccccatctcg gcctcgtcga gcgacttcag gacatagacc
     1501 gaagcccgcg acaacagcgc cgaattgacc tcgaacgacg ggttttccgt cgtcgcaccg
     1561 atgaaggtga acaacccgct ttcgacaaac ggcagaaagc cgtcctgctg cgacttgttg
     1621 aagcggtgga tttcatcgac gaagaggatg gtccgcttgc cctgcgcctt ccacagttcg
     1681 gcctgcgcca ccgcctcgcg cacttccttg atcccggaaa acaccgccga cagcgcaatg
     1741 aactcgcact ggaactgcgt cgccatcatc cgcgccagcg tcgtcttgcc aacgcccggc
     1801 ggtccccaca ggatcatcga atgcggctgg ccggacgcaa acgccaggcg caagggctta
     1861 cccggcccga gcagatgctg ctggccgatc acctcgtccg gcgtctgcgg gcgcaactgt
     1921 tcggccagcg gcgcggaacg gtcgacttcg gt
//
LOCUS       DAOSDX010000597         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_19992_length_1951_cov_2.215190, whole genome shotgun sequence.
ACCESSION   DAOSDX010000597 DAOSDX010000000
VERSION     DAOSDX010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19992_length_1951_cov_2.215190"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="thpR"
                     /locus_tag="PLN02_11995"
     CDS             complement(<1..334)
                     /gene="thpR"
                     /locus_tag="PLN02_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOI53093.1"
                     /translation="MAAESARVFFALWPPAALADDLAGLAGQLAPTCGGRPTRADTVH
                     LTLAFVGDIDASRLPELAAAARGLAVTPFTLTLDRLGYWPHNHIVWAGCSAPPAGLGE
                     LAATLREQV"
     gene            complement(324..806)
                     /gene="ispF"
                     /locus_tag="PLN02_12000"
     CDS             complement(324..806)
                     /gene="ispF"
                     /locus_tag="PLN02_12000"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOI53094.1"
                     /translation="MNIRVGQGYDVHKLVEGRKLILGGVEIDHPTGLLGHSDADALLH
                     AITDALLGAAALGDIGRHFPDTDPRYAGADSRVLLRAAVALLAEKGWRPVNVDATLIA
                     QQPKLAPHAPAMVANIAADLGIGVDCVNLKGKTNEKLGYLGREEAIEAQAVVLVVRGG
                     "
     gene            complement(803..1489)
                     /gene="ispD"
                     /locus_tag="PLN02_12005"
     CDS             complement(803..1489)
                     /gene="ispD"
                     /locus_tag="PLN02_12005"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOI53095.1"
                     /translation="MPRHYAIVPAAGSGARFGAEKPKQYLDLLGRPLIFHTLAALTAH
                     PAIERVWVVLAPDDPWWPRHDWSELGPKLETVRCGGATRAESVGNGLQAAAMAAADDD
                     WILVHDAARPCLSRRMLDALCDELADDPVGGLLAVPVADTLKRADANQRVAATEPRDG
                     LWQAQTPQMFRYGRLVEALNKHNAVTDEAGAIEALGLTPKLVRGDSTNLKVTFPADLA
                     LAAMILRGRK"
     gene            1531..>1951
                     /locus_tag="PLN02_12010"
     CDS             1531..>1951
                     /locus_tag="PLN02_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOI53096.1"
                     /translation="MSFVVPELAAPVEAFATALGIGLLIGMERERRPDSAAGLRTFSL
                     VAMLGCLFAMLGEKSGGPWLLAVGLVVVAASMVASNFSSQQEEQYRGFTSEAAIIVTY
                     GLGAAVWLGYAQVAVMLALTTTMLLYFKTELKAFSESM"
BASE COUNT          302 a          644 c          678 g          327 t
ORIGIN      
        1 tcacctgctc gcgcagggtc gccgccagtt cgcccagccc ggccggcggc gcgctgcaac
       61 cggcccagac gatgtggttg tgcggccagt aaccgaggcg atcgagcgtc agcgtaaacg
      121 gcgtgacggc cagcccccgc gcggcggcgg cgagttccgg caagcgtgat gcgtcaatgt
      181 cgccgacgaa ggccagcgtc agatgcacgg tgtcggcgcg tgtcggcctg ccgccgcaag
      241 tcggtgccag ttgcccggcc agcccggcga ggtcgtcggc cagcgcggcc ggtggccaga
      301 gggcgaagaa aacgcgggcg ctttcagccg ccacggacga cgaggacgac cgcctgcgcc
      361 tcgatcgctt cctcgcggcc gaggtagccg agcttttcgt tggtcttgcc cttgaggttg
      421 acgcaatcga cgccgatgcc gaggtcggcg gcgatgttgg cgaccatcgc cggtgcgtgc
      481 ggcgccagct tcggctgctg ggcgatcagc gtcgcgtcga cattgaccgg ccgccagcct
      541 ttctcggcga gcagcgcgac ggcggcgcgc agcaggacgc ggctgtcggc gcctgcatag
      601 cgcgggtcgg tatcgggaaa atggcggccg atgtcaccga gcgctgcggc gccgagcagc
      661 gcgtcggtga tcgcatggag cagcgcgtcg gcgtcggaat ggccgagcaa gccggtcgga
      721 tggtcgatct cgacgccgcc gaggatcagc ttgcggcctt cgaccagttt atgtacgtcg
      781 tagccctgcc cgacccggat attcatttgc gtcctctcag gatcatcgcg gccagcgcca
      841 ggtcggccgg aaaagtgact ttcaggttgg tcgaatcgcc gcgcaccagt ttcggcgtca
      901 ggcccagcgc ttcgatggcg ccggcttcgt cggtgacggc attgtgcttg ttcagcgcct
      961 cgaccagtcg accgtagcgg aacatctgcg gtgtctgcgc ctgccacagg ccgtcgcgcg
     1021 gctcggtggc ggcgacgcgc tggttggcgt cagcgcgttt cagcgtatcg gcgaccggca
     1081 ccgcgaggag accgccgacc ggatcgtcgg ccagttcgtc gcacagcgcg tcgagcatcc
     1141 gccggctgag acaggggcgc gccgcgtcat ggaccagaat ccagtcgtcg tcggctgccg
     1201 ccatcgccgc cgcctgcagt ccgttgccga cgctttcggc gcgcgtggcg ccgccgcaac
     1261 gcacggtttc cagcttgggc ccgagttccg accagtcgtg gcgcggccac cacggatcgt
     1321 ccggcgccag caccacccag acgcgttcga tcgcggggtg cgcggtcaac gccgccaaag
     1381 tgtggaaaat cagcggccgg ccgagcaggt cgaggtactg tttcggcttc tcggcgccga
     1441 agcgcgcgcc gctgccggca gcgggaacga ttgcgtaatg tctgggcatg gcttaatttt
     1501 aacgaagaac aaggaaaaga gaggcaagcc atgagtttcg tcgttcccga actggccgca
     1561 ccggtcgagg cattcgccac cgcgctcggc atcggcctgc tcatcggcat ggagcgcgaa
     1621 cggcggccgg actccgccgc cggactgcgc accttctcgc tggttgccat gctcggctgc
     1681 ctgttcgcca tgctcggcga gaaaagcggc ggaccgtggc tgctggcagt cggcctcgtt
     1741 gtcgtcgcgg catcgatggt cgcctccaat ttctcgtcgc aacaggagga gcaataccgc
     1801 ggcttcacct cggaagcggc gatcatcgtc acctacggcc tgggtgccgc cgtctggctg
     1861 ggctatgccc aagtggcggt gatgctggcg ctgaccacca ccatgctgct ttatttcaag
     1921 accgaactga aagcattcag cgaaagcatg a
//
LOCUS       DAOSDX010000598         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20005_length_1950_cov_2.453826, whole genome shotgun sequence.
ACCESSION   DAOSDX010000598 DAOSDX010000000
VERSION     DAOSDX010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20005_length_1950_cov_2.453826"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLN02_12015"
     CDS             complement(<1..685)
                     /locus_tag="PLN02_12015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI53097.1"
                     /translation="MEKTTHFGYQEVAESAKAAKVAEVFHSVATRYDLMNDLMSAGLH
                     RLWKRFAVAQAGLRPGMKVLDVAGGTADLTRLFLKEVGDSGTVLLTDINFSMLREGRD
                     RMLNEGRTPPAVQCDGERLPFPADHFDCVSVAFGLRNMTHKDQALAEMYRVLKPGGRL
                     LVLEFSRVWKPLEPAYDLYSFKLLPLIGELVAQDRDSYQYLAESIRMHPGQDELKAMM
                     EAAGFARVRY"
     gene            809..1504
                     /gene="phoB"
                     /locus_tag="PLN02_12020"
     CDS             809..1504
                     /gene="phoB"
                     /locus_tag="PLN02_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HOI53098.1"
                     /translation="MAVNILLVEDEPGIQELLKFNLTQAGHRITVTGDAEQALQFLRH
                     GLPDVILLDWMLPGMSGIDLCRRLRSDERYQPVPIIMLTARGEERDKVLGLDTGADDY
                     ITKPFSPRELVSRIKAVLRRRAPQMTGEPVEIDGLRLDPASHRVQGGGQPLELGPTEF
                     RLLHFFMTHPERVHSRTQLLDQVWGDDVFVEERTVDVHIRRLRLALEPSGHAGLIQTV
                     RGAGYRFSAGVGR"
     gene            1536..>1950
                     /locus_tag="PLN02_12025"
     CDS             1536..>1950
                     /locus_tag="PLN02_12025"
                     /inference="COORDINATES: protein motif:HMM:NF023236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HOI53099.1"
                     /translation="MGFWWRPLGVLAGALCVALILWAASGWVAALGFLAGIQAIVMLR
                     RLWQEFRLSRWLEHPDEVEPPDATGTWGDIFYRLQKLQRRQKASRSELSTALEQFEHA
                     AQAVPDAMVILNGNEQIEWCNPASRTYLGLDSERDR"
BASE COUNT          323 a          652 c          646 g          329 t
ORIGIN      
        1 ggtagcgcac gcgggcgaag ccggccgcct ccatcatcgc cttgagttcg tcttgaccgg
       61 ggtgcatgcg gatcgactcg gccagatact ggtagctgtc gcggtcctgc gcgacgagtt
      121 cgccgatcag cggcagcagc ttgaaggaat acagatcgta ggccggctcc agcggcttcc
      181 acaccctgga aaattcgagg acaaggagcc ggccgccggg cttgagcacg cggtacatct
      241 cggcgagcgc ctgatccttg tgcgtcatgt tgcgcagccc gaacgccacc gatacgcagt
      301 cgaaatggtc ggcgggaaag ggcaggcgct cgccgtcgca ctgcaccgcg ggcggtgtgc
      361 ggccctcgtt cagcatgcgg tcgcggcctt cgcgcagcat ggagaagttg atgtcggtga
      421 gcagcacggt gccgctgtcg ccgacttcct tcaggaacag ccgcgtcagg tcggcggtgc
      481 cgccggcgac gtcgagcacc ttcatgcccg gccgcaggcc ggcctgcgcc acggcgaagc
      541 gcttccacag ccggtgcaga ccggccgaca tgaggtcgtt catgaggtcg tacctggtgg
      601 cgaccgaatg gaagacctcg gccaccttgg cggccttggc ggactcggcg acttcctgat
      661 agccgaaatg ggtggttttt tccatgatgg ggacggcgag cgggcaagct tgaaattcta
      721 ccagcacggc tttccagggg gttgcggcgt cacaaatcgg tcatactgcg gccataacat
      781 ggccgctgtt cacccaggat cgaatctcat ggctgtcaac attctgctgg tcgaggacga
      841 gccgggcatc caggaactgc tgaagttcaa tctcacgcag gcgggccacc ggatcaccgt
      901 caccggcgat gccgagcagg cgctgcaatt cctccgccat ggcctgcccg acgtcatcct
      961 gctcgactgg atgctgcccg gcatgtcggg catcgacctg tgccgtcgcc tgcgcagcga
     1021 cgagcgctac cagccggttc ccatcatcat gctgaccgcg cgcggcgagg agcgcgacaa
     1081 ggtgctgggg ctcgacaccg gcgccgacga ctacatcacc aagccgtttt ccccgcgcga
     1141 actggtgtcg cgcatcaagg ccgtgctgcg ccgccgcgca ccgcagatga ccggcgagcc
     1201 ggtggagatc gacggcctgc gtctcgaccc agcgagccac cgcgtgcagg gtggcggaca
     1261 gccgctcgaa ctcgggccca ccgaattccg cctgctgcat tttttcatga cgcaccccga
     1321 gcgggtccat tcccgcaccc agttgctcga ccaggtgtgg ggcgatgacg tcttcgtcga
     1381 ggagcgcacc gtcgacgtcc acatccgccg cctgcgcctg gcactggagc cgtccggcca
     1441 cgccggcctg atccagacgg tgcgcggcgc gggttatcgc ttttctgccg gagtcggccg
     1501 ataatggggt ccggttcgta agcgacggga agacgatggg attctggtgg cggccgctcg
     1561 gcgtgctggc cggggcgctg tgcgtggcgt tgatcctgtg ggcggcctcc ggctgggtgg
     1621 ccgcgctcgg ctttctcgcg ggcatccagg ccatcgtcat gctgcgccgg ctgtggcagg
     1681 aattccgcct gtcgcgctgg ctggaacacc ccgacgaagt cgagccgccc gacgccaccg
     1741 gcacctgggg cgacatcttc taccgcctgc agaaactcca gcgccgccag aaggcgagcc
     1801 gcagtgaact gtccaccgcc ctcgaacagt tcgaacatgc cgcccaggcc gtgcccgacg
     1861 ccatggtcat cctcaatggc aacgagcaga tcgaatggtg caaccccgcc tcgcgcacat
     1921 acctgggact cgatagcgag cgcgatcgcg
//
LOCUS       DAOSDX010000599         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20032_length_1948_cov_2.547808, whole genome shotgun sequence.
ACCESSION   DAOSDX010000599 DAOSDX010000000
VERSION     DAOSDX010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20032_length_1948_cov_2.547808"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..>1948
                     /locus_tag="PLN02_12030"
     CDS             85..>1948
                     /locus_tag="PLN02_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53100.1"
                     /translation="MIAMAVPGIRQVADARQRLMANPEAQAAWAALDCGFASVADVFD
                     DCYAAAKQALTDDELPAFLETARRLGKLGRGAEPPLAFLEGWPRVVATIPQDEALPLL
                     LETIGELQKSPNGPALTPLLQTLGGVAERLRSALALEHYLRLARDLAARTSGSIHGRQ
                     VTQASPALPEFLRVAPQLLALVPLAGVRRWLEDGIRLSGEHPERLREYFALRSPESRA
                     LLQQQRAGTLFADVERRLALSQQSLWQDDSRLVPYSTAFVAQGQQPYLAADGMRLPDV
                     LDERAGVSGSDRYRAMLAHLSCHRRWSTPLVADNWSPLQRLSVEAFEDARIDALILRR
                     YPGLRRILLTLHPIPAADACDPARENTLRHRLTRLSRACIDRDYRDPDPLINEFANRF
                     AAQLAAGAASTRAMAELALAYASRSRLPSDQFAQVHFADTEVDYRDDNRHLWTFIEAG
                     DEEESFTPPPRAADPEASGLPPRHYPEWDAASQSYRPDWAAVYEALHPAGRAADIDAL
                     LARHAVLARRLKRLLDLVKPQARVRERYRENGSELDLDVAIDALVDWQSGRLPEARIE
                     ISQRTAGRDISVLLLLDLSQSLNATLPGSGQRVLDTAREAVALLAWAIDQLGDAC"
BASE COUNT          290 a          780 c          606 g          272 t
ORIGIN      
        1 ctgccggatg gcgatggtgc gcccgatcac cgacgacccg gacatcatcg acagtctcga
       61 ccacgccatc ggcgcgattt tcggttgatc gccatggccg tccccggcat ccgccaagtg
      121 gccgacgccc gccagcggct gatggccaac cctgaagcgc aggcagcctg ggcggcgctc
      181 gattgcggtt ttgccagcgt cgccgacgtt ttcgacgact gttacgcggc ggcgaagcaa
      241 gcgctcaccg acgacgaact ccccgccttt cttgaaaccg cccgccggct cggcaaactt
      301 ggccggggcg ccgaaccgcc gctggccttt ctcgaaggct ggccgcgcgt ggtggcgacg
      361 atcccccagg acgaggcttt gccactgctc ctcgagacca tcggcgagtt gcagaaatcg
      421 cccaacggcc cggccctgac gccgctgctg caaaccctgg gcggcgtcgc cgaacgcctg
      481 cgcagcgcac tcgccctcga acattacctg cgtctggcgc gcgatctggc ggcacgcacc
      541 tcgggctcga tccacggccg ccaggtcacc caggccagtc cggcactgcc cgagttcctg
      601 cgcgtcgcac cgcagctcct cgccctggtc ccgctggccg gcgtccggcg ctggctggaa
      661 gacggcattc gtctgagcgg cgagcacccg gagcgcctgc gcgagtattt cgcgctgcgc
      721 tcgccggaaa gccgcgccct cctccagcaa cagcgcgccg gcaccttgtt cgccgacgtc
      781 gagcgccgcc tcgccctcag ccagcagagc ctgtggcagg acgacagccg gctcgttccg
      841 tactcgaccg ccttcgtcgc ccaaggccag cagccctacc tggccgccga cggcatgcgc
      901 ctgccggacg tcctcgacga gcgcgccggg gtatccggca gcgaccgcta ccgggcgatg
      961 ctcgcccacc tgagctgcca ccgccgctgg agcacgccgc tcgtcgccga caactggagc
     1021 ccgctgcagc gcctgagcgt cgaggccttc gaggatgccc gtatcgacgc gttgatcctt
     1081 cgccgctatc ccggcctgcg ccggattctg ctgacgctgc atccgatacc ggccgccgac
     1141 gcctgcgacc cggcccgcga gaataccttg cgccaccgcc tgacccggct gtcgcgcgcc
     1201 tgcatcgacc gcgattaccg cgaccccgac ccgctgatca acgagttcgc caaccgtttc
     1261 gccgcgcaac tggcggccgg cgccgcctcg acccgggcga tggccgaact ggcgctcgcc
     1321 tatgccagcc gcagccggct gcccagcgac cagttcgccc aggtgcattt cgccgacacc
     1381 gaggtcgatt accgcgacga caaccgccat ctgtggacct tcatcgaggc cggcgacgag
     1441 gaggaatcct tcaccccgcc gccgcgcgcc gccgaccccg aagcgagcgg cctgccgccg
     1501 cgtcactacc ctgaatggga tgccgccagc cagagctacc ggccggactg ggcagcggtc
     1561 tacgaagccc tgcacccggc cgggcgcgcc gccgacatcg acgcgctgct ggcccgccac
     1621 gccgtactcg cccgccgcct caagcgcctg ctcgacctgg tcaagccgca ggccagggtg
     1681 cgcgagcgct accgcgaaaa cggcagcgaa ctcgacctcg acgtcgccat cgacgccctc
     1741 gtcgactggc agagcggccg tctgcccgaa gcccgcatcg aaatcagcca gcgcactgcc
     1801 ggccgcgaca tcagcgtgct cctgctgctc gacctctcgc aatcgctcaa cgccacgctg
     1861 ccgggcagcg ggcagcgcgt gctcgacacc gcgcgcgagg cggtcgccct gctcgcctgg
     1921 gccatcgatc agctcggcga cgcctgcg
//
LOCUS       DAOSDX010000600         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20038_length_1947_cov_4.131078, whole genome shotgun sequence.
ACCESSION   DAOSDX010000600 DAOSDX010000000
VERSION     DAOSDX010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20038_length_1947_cov_4.131078"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLN02_12035"
     CDS             complement(<1..211)
                     /locus_tag="PLN02_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53101.1"
                     /translation="MSALIRSPGILRMVVALFLAGWLSAAGAQQAPLKLGIMPFNSPL
                     ALIRTHQPLTEHLERQLGRKVIDLTS"
     gene            396..1118
                     /locus_tag="PLN02_12040"
     CDS             396..1118
                     /locus_tag="PLN02_12040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI53102.1"
                     /translation="MRPDVDKVIVSPRATPSIALPDGCRKRGQWLEHLIKVHGWSCGA
                     ELGVWRGETFTHLLANCAGLSCLYGVDLWASQPDNDGPESYIDWPHDENERRVRAVAA
                     RHGNRGRVLKMWTHEAARLVPDESLDFVYIDADHSEQGVRRDIADWLPKVKATGWILG
                     HDINWDTVRRAVDDLLPGYVIGPDNAWGRPKSEVAAGIAAYLAVRGVPVSRRHKTLVQ
                     RLRQWLRLNKLRDAQPRPGGVA"
     gene            complement(1091..>1947)
                     /locus_tag="PLN02_12045"
     CDS             complement(1091..>1947)
                     /locus_tag="PLN02_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOI53103.1"
                     /translation="PMRVGSFEFLCRGCLTAPTLAEALQRASRFLHLVLPDLAVSVRR
                     SQGRADLVIAETRHLAADPDDPGRIFAFEWLLRLLHGLSCWLAGRGIGLDSVIFPYRK
                     PVHFADYALIFTEDSHFAPTLPGGKGVLLASFNANLLELPLRRDEAALTAFLDGAPGK
                     ITTLYRRDRAMVIRVRDLLRAALPVPLSLDEVAERLHLSPRTVHRRLEEEGSSFRGIK
                     DALRRDMALARLVKTRDPVARIAADLGYTDTSAFYRAFVEWTGMAPAYYRRHWVENGD
                     QATPPGLG"
BASE COUNT          329 a          583 c          712 g          323 t
ORIGIN      
        1 gcgaggtaag gtcgatgacc ttgcggccga gctggcgctc cagatgttcg gtcagcggct
       61 ggtgggtgcg gatcaacgcc agcgggctgt tgaaaggcat gatgccgagc ttgagcggtg
      121 cctgctgggc gccggcggcg gacagccagc cagcgaggaa cagcgccaca accatccgca
      181 ggatgccggg agaacgaatc agggcggaca tggcgatctt ccttttgctg acggcacaag
      241 ggtaacgctg caattttcct cctgtagtgg ctggcgggca accgtgctga acatcaaccg
      301 gtaacacagt ttcgctagcc tgcatgtttc agccagtttc cacatgcgtg ccgcagggat
      361 cgtgttccgg cagccgtgct tgaagagggg gagcaatgag acctgacgta gacaaagtga
      421 ttgtttcacc gcgggccaca cccagcattg cgttgccgga cgggtgccgg aagcgtggcc
      481 agtggctcga gcatctgatc aaggtgcatg gctggtcatg cggtgccgaa ctgggggtct
      541 ggcggggtga gaccttcacc catctgctcg ccaattgcgc cggcttgagt tgcctttacg
      601 gcgtcgatct ctgggcgtcg cagccggata acgacggccc cgagtcctac attgactggc
      661 cgcacgatga gaacgagcga cgtgtccgcg ccgttgccgc tcgtcatggc aatcgcggac
      721 gggtcctgaa gatgtggacc cacgaggcgg cgcgcctggt gcctgacgag agtctcgatt
      781 tcgtctatat cgatgcagat cacagcgagc aaggcgtgcg ccgggatatc gcggactggc
      841 tgccgaaggt caaggcgacg ggctggatac tggggcacga catcaactgg gatacggtca
      901 ggcgtgctgt cgacgatctg ctgccgggct atgtgatcgg ccccgacaat gcctggggcc
      961 gccccaagtc ggaggttgcg gcgggtatcg ccgcctacct ggcggtacgt ggcgtgccgg
     1021 tcagccggcg gcacaagacg cttgtccagc gcctgcgtca gtggctgcgc ctcaacaagt
     1081 tgcgggatgc tcagcccagg cctggcggcg tcgcctgatc gccgttctcc acccagtgtc
     1141 ggcgatagta ggccggcgcc atgccggtcc attcgacgaa ggcgcgatag aaggccgagg
     1201 tatcggtata gccgagatcg gcggcgatcc gggcgaccgg gtcgcgggtc ttgaccaggc
     1261 gggccagcgc catgtcgcga cgcagcgcgt ccttgatgcc gcggaagctt gagccttcct
     1321 cctccagccg gcggtgtacc gtgcgcggcg agaggtgcag gcgttccgcc acttcgtcga
     1381 ggctcagcgg gacgggcagg gcggcgcgca gcaggtcgcg gacgcggatc accattgccc
     1441 ggtcgcgccg gtagagggtc gtgatcttgc ccggcgcgcc gtcgaggaag gcggtcagcg
     1501 ccgcctcgtc gcggcgcagc ggcagttcga gcaggttggc gttgaagctg gcgagcagta
     1561 cgcccttgcc gccgggcagg gtcggcgcga aatgcgaatc ttcggtgaag atcagcgcgt
     1621 agtcggcgaa atgcaccggt ttgcggtacg ggaagataac gctgtcgagg ccgatgccgc
     1681 gcccggcgag ccagcacgac aggccgtgca gcaggcgcag cagccattcg aaggcgaaga
     1741 tgcgcccggg gtcgtcgggg tcggcggcga ggtggcgggt ttcggcaatc accaggtcgg
     1801 cgcgcccctg gctgcgccgg acgctgaccg ccaggtcggg cagcaccagg tgcaggaagc
     1861 ggctggcgcg ctgcagcgct tcggccagcg tcggcgccgt caggcagccg cggcagagaa
     1921 attcgaagct gccgacgcgc atcggct
//
LOCUS       DAOSDX010000601         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20076_length_1944_cov_3.474854, whole genome shotgun sequence.
ACCESSION   DAOSDX010000601 DAOSDX010000000
VERSION     DAOSDX010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20076_length_1944_cov_3.474854"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1776)
                     /locus_tag="PLN02_12050"
     CDS             complement(121..1776)
                     /locus_tag="PLN02_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOI53104.1"
                     /translation="MNSRSSFLLPRLSPLLVVGLVVAALAAMPVSSVGLNLFVGGTSE
                     TWAHLAQTVLSEYIANSLWLCLGVAVGVAVVGVTTAWLTAMHDFPGRRVFEWAMVLPL
                     AVPAYVMAYVYTDFLQFVGPLQTALRETFGWEHGDYWFPDIRTLPGAVLMFVSVLYPY
                     VYLLARSAFLERASGMLEAARTLGMGPWRAFFSVSLPLARPAVVAGIALALMETLADY
                     GTVAYFAVNTFTTGIYRAWFSLGDRVAAAQLAAMLLGFVLLLLMVERVSRGRARYHDT
                     TGRNRPLAGMRLTGIRAWLASLLCLLPLLLGFLLPAGLLLKMALTEGDAQFGERFLVL
                     SRNSFVLAGTTALLGVLLALLLAYGARLAKGGLAAGLNRLVGLGYAVPGAVIAVGVLI
                     PVTRLDQWLAGQWESWFGNNPGLLLTGGIAALVYAYLVRFLAVALHTVESGLGKITSS
                     MDDAARSLGLGQGASLRRVHAPLLRGSLLTAGLLVFVDVMKELPATLVMRPFNFDTLA
                     TQAYTLASDERLAEASTASLAIVAVGILPLILLSRQIAASRRR"
     gene            1801..>1944
                     /locus_tag="PLN02_12055"
     CDS             1801..>1944
                     /locus_tag="PLN02_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI53105.1"
                     /translation="MAQLELTHILQRYGTHLVVDGVGFRLETGSIACLLGPSGCGKTT
                     LLRC"
BASE COUNT          437 a          665 c          597 g          245 t
ORIGIN      
        1 aagggggtct atggcgtgga caccaacgtt ctggccttgg acttcgagcg ctcgggcggc
       61 gcctgggaaa agaccgaccc gacgccgcct gcctcgatcg acgacaagcc aaagtaagtc
      121 tcagcgacga cggcttgccg caatctggcg cgagagcagg atcagcggca ggatgccgac
      181 ggcgacgatg gccagcgacg cggtagacgc ttccgccagc cgctcgtcgg aagccagggt
      241 ataggcctgc gtcgccagcg tatcgaaatt gaacgggcgc atcaccagcg ttgccggcaa
      301 ttccttcatc acatcgacga aaaccagcaa gcctgcggtc agcagactgc cgcgcaacag
      361 cggcgcatgc acccggcgca ggcttgctcc ctgaccgagc ccgagactgc gggcggcgtc
      421 gtccatgctg gaagtgatct tgcccagccc ggactccacg gtatgcagcg ccaccgccag
      481 gaaacgcaca agataggcgt agaccagggc ggcgatgccg ccggtcagca gcagacccgg
      541 gttgttgccg aaccagcttt cccactggcc ggccagccat tgatcgagcc gcgtcaccgg
      601 aatcagcacg ccaaccgcaa tcaccgcccc cggcaccgca taaccgaggc cgaccaagcg
      661 attcagcccg gccgccagcc ctcccttcgc cagccgcgcc ccgtacgcca acaacagcgc
      721 cagcaggaca ccgagcagcg cggtcgtccc cgccagcaca aaactgttgc gcgagaggac
      781 caggaaacgt tcgccaaact gggcatcgcc ttcggtcagg gccattttca acagcaagcc
      841 ggcaggcagc aggaagccga gcagcaacgg cagcagacac aagagggaag ccagccaagc
      901 ccggatcccg gtcagacgca taccggccag cggacgattg cgcccagttg tatcgtgata
      961 gcgggcacgc ccgcgcgaca cccgctcgac catcagcagc agcagcacga aaccgagcag
     1021 catggccgcc agctgggccg ccgcaacgcg gtcgccgagc gagaaccagg cacggtagat
     1081 gccggtcgtg aaggtgttca ccgcgaaata ggccaccgtt ccatagtcgg ccaatgtttc
     1141 catcagggcc agcgcgatgc cagcgacaac cgccggacgg gcgagcggta gagacaccga
     1201 aaagaacgcg cgccacggcc ccatgcccaa ggtccgcgcc gcctcgagca tgccgctcgc
     1261 ccgctcgagg aaggcgctgc gcgccagcag atagacgtaa ggatagagaa cgctgacgaa
     1321 catcagcacc gcgccgggta aggtacgaat gtcggggaac cagtaatcgc cgtgttccca
     1381 gccgaaagtc tcgcgcagag cggtctgcag cggaccgacg aactgcagga aatcggtata
     1441 gacatacgcc atcacgtaag ccgggactgc cagcggcaaa accatcgccc attcgaagac
     1501 gcgccgcccg ggaaaatcgt gcatcgccgt cagccaggcg gtcgtcacgc cgaccacggc
     1561 aaccccgacc gcaaccccca gacacagcca caacgaattg gcgatgtact cgctcagcac
     1621 cgtctgcgcc agatgggccc aggtttcgct ggtcccgccg acgaacaggt tgagaccgac
     1681 gctgctcacc ggcatggcgg caagcgccgc gacgacgaga ccgacgacga ggagcggcga
     1741 gagccgcggg agaaggaagc ttgagcgcga gttcatgaat gcgaattata atcgagcacc
     1801 atggcacagc tcgaattaac ccacattctt cagcgctacg gcacacacct ggtggtcgac
     1861 ggtgtcggtt tccgtctcga aaccggcagc atcgcctgtc tgctcggccc ctcgggctgc
     1921 ggcaagacga ccttgctgcg ctgc
//
LOCUS       DAOSDX010000602         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20115_length_1942_cov_2.656598, whole genome shotgun sequence.
ACCESSION   DAOSDX010000602 DAOSDX010000000
VERSION     DAOSDX010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20115_length_1942_cov_2.656598"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLN02_12060"
     CDS             complement(<1..627)
                     /locus_tag="PLN02_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253728.1"
                     /note="uses the energy from reduction of ubiquinone-1 to
                     ubiquinol to move Na(+) ions from the cytoplasm to the
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOI53106.1"
                     /translation="MIRIKRGLDLPITGAPEQRIEAARPVRSVAVIGFDYHGMKPTMA
                     VQVGDRVKLGQVLFSDKKTPGVNFTAPGAGVVSAIHRGEQRVLQSVVIELEGDEAISF
                     ACYPEAQIDRLSSEMVRDNLQQSGLWTALRSRPFSRVPAVDAVPASIFVTAIDTHPLA
                     ADPAVVIAEAAADFTRGLRVLARLARVFLCVADGAAIPGSGLEQVQVES"
     gene            complement(877..970)
                     /locus_tag="PLN02_12065"
     tRNA            complement(877..970)
                     /locus_tag="PLN02_12065"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(934..936),aa:Ser,seq:gct)
     gene            complement(1028..>1942)
                     /locus_tag="PLN02_12070"
     CDS             complement(1028..>1942)
                     /locus_tag="PLN02_12070"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOI53107.1"
                     /translation="LTDNTFTKARILSIDEENIRRDLDAGKVVIVAGFQGVDADGNIT
                     TLGRGGSDTSGVALAAALKADECQIYTDVDGVYTTDPRVVPEAKKLDTITFEEMLEMA
                     SLGSKVLQIRSVEFAGKYKVKLRVLSSFEDEGEGTLITVEEDKNMEQPIISGIAFNRD
                     EAKLTMLGVPDTPGIAYRILGSIADANIDVDMIIQNIGHDGTTDFSFTVNRGEYAKAQ
                     AILEKVKAELGAREIVGDNKICKVSAVGVGMRSHPGVASKMFKALGDEGINIQMISTS
                     EIKISVVIDEKYLELAVRVLHRTFGLDQ"
BASE COUNT          363 a          615 c          581 g          383 t
ORIGIN      
        1 ggactcgacc tgaacttgct ccaggccgct gccgggaatc gcagcgccgt cggcaacgca
       61 caggaaaacc ctggccaggc gcgccagcac gcgcaacccg cgggtgaaat cggccgccgc
      121 ttcggcgatc acgacagccg gatcggcggc cagcggatgg gtatcgatcg ccgtcacgaa
      181 aatcgacgcc ggcaccgcat cgacggccgg cacccggctg aagggacggc tccgcaacgc
      241 ggtccacaac cccgactgct gcaggttgtc gcgcaccatt tcgctactca accgatcgat
      301 ctgcgcctcc ggatagcagg cgaagctgat tgcctcgtcg ccttccagtt cgatcaccac
      361 cgattgcagc acccgctgct cgccgcgatg aatggcactg acgacgccgg cacccggcgc
      421 cgtgaaattg acgcctggcg tcttcttgtc ggagaacagc acctgcccca gcttgacccg
      481 atcaccgacc tgtacggcca tcgttggctt catcccgtgg tagtcgaagc cgatgaccgc
      541 cacactgcgt accggcctgg cggcctcgat acgctgctca ggcgccccgg tgatgggcaa
      601 gtccagaccg cgtttaattc tgatcattcg cttagcccga attgaaattg accacgcaag
      661 gcgggaattc atctcgcccg gactctgccc cggacgccgc tcccgtcctt gcgaccttgc
      721 atgcaacaac gtcaaagccc gggagtttac cggcgacagc ggcaggctgg aaccgccggg
      781 ctacagataa tggaatcgtg atattccatc aggcagggca atacgaatgc gctggacgaa
      841 aaaaaaacac cctgacgggt gtttttcttc gtcgtctggc ggagacggag ggattcgaac
      901 cctcgataca cgttttagcg cgtatgctcc cttagcaggg gagtgccttc gacctctcgg
      961 ccacgtctcc ggttcgttca acaacgaggc gcggatgata tagcctccgc gcccgttggt
     1021 caaaccttta ctgatccaga ccgaaagtgc ggtgcagcac gcgcacggcg agttccagat
     1081 acttctcgtc gatgacgacg gagatcttga tttccgaggt cgaaatcatc tggatgttga
     1141 tgccttcgtc acccagcgcc ttgaacatct tcgacgcgac gcccgggtgc gagcgcatgc
     1201 cgacgccaac cgccgaaacc ttgcagatct tgttgtcgcc gacgatttcg cgggcgccca
     1261 gttcggcctt gaccttttcc agaatggcct gggccttggc gtactcgccg cgattcaccg
     1321 tgaaggagaa atcggtcgtg ccgtcgtgac cgatgttctg gatgatcatg tcgacgtcga
     1381 tgttggcgtc ggcgatcgag ccgaggatgc ggtaggcgat gcccggggta tccggaacgc
     1441 ccagcatggt cagcttggct tcgtcgcggt tgaaggcgat gccggaaatg atcggttgtt
     1501 ccatgttctt gtcttcctca acagtgatca gcgtaccctc cccttcgtcc tcgaagctgg
     1561 aaagaacgcg cagtttgacc ttgtacttgc cggcgaattc gaccgagcgg atctgcagca
     1621 ccttcgagcc gaggctggcc atttccagca tttcctcgaa ggtgatggtg tcgagcttct
     1681 tcgcttccgg cacgacgcgc gggtcggtgg tgtacacacc atcgacgtcg gtgtagatct
     1741 ggcactcgtc ggccttcagc gcggccgcca gggcaacgcc ggaggtgtcc gaaccgccgc
     1801 ggccgagcgt cgtgatgttg ccgtcggcgt ccacgccctg gaaaccggca acgatgacga
     1861 ccttgccggc atcgagatcg cggcggatgt tctcctcgtc gatcgacagg atgcgcgcct
     1921 tggtgaaggt gttgtcggtc ag
//
LOCUS       DAOSDX010000603         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20147_length_1940_cov_2.681167, whole genome shotgun sequence.
ACCESSION   DAOSDX010000603 DAOSDX010000000
VERSION     DAOSDX010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20147_length_1940_cov_2.681167"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="ispB"
                     /locus_tag="PLN02_12075"
     CDS             <1..895
                     /gene="ispB"
                     /locus_tag="PLN02_12075"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HOI53108.1"
                     /translation="YSDVVLVRQVAEYIINSGGKRMRPALVLLTAGAMGYQGTHHHEL
                     AAVVEFIHTATLLHDDVVDESELRRGRQTANAAFGNAASVLVGDFLYSRAFQMMVAVD
                     NMRVMQVLSDATNIIAEGEVLQLMNCHDADVDEERYMQVIHYKTAKLFEAAARLGGII
                     GDAEPVVEAALASYGMHLGTAFQLVDDVLDYSGQEADTGKHLGDDLAEGKPTLPLIHA
                     MQHGTPEHAACVRKAIEEGGRDAFPEVLQAIQQSGALEHTRARAAKEAEQARAAISLL
                     GDSLYKKALLELTFLAVERNH"
     gene            912..988
                     /locus_tag="PLN02_12080"
     tRNA            912..988
                     /locus_tag="PLN02_12080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:946..948,aa:Pro,seq:cgg)
     gene            complement(1122..>1940)
                     /locus_tag="PLN02_12085"
     CDS             complement(1122..>1940)
                     /locus_tag="PLN02_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI53109.1"
                     /translation="ERMGRGVALTGAGELLVHHALRILGEIKDAEASLQALNGAESGQ
                     ISVGLVSTAKYFMPRLLAKFAQQHPGVDVQFSVGNRQALLQKLQDNAIDLAIMGRIPQ
                     EIDAHGEPMACHPYVLIAPADHPLRDARRFEMHELRHETFLLREEGSGSRRVAEEMFK
                     NHLFTPARTISMGSNETIKQAVMAGMGVSLLSLHTLPLELKTHALCLLDVVGTPIERT
                     WYVVNMSAKRLMPAAAKFRAFLLDETAPALIQEFDHHLLNLERQPCPYDVGGKG"
BASE COUNT          360 a          572 c          620 g          388 t
ORIGIN      
        1 ctactccgac gtcgtgctgg ttcgccaagt cgccgaatac atcatcaaca gtggcggcaa
       61 gcgcatgcgc ccggcactgg tgctgctgac tgccggggcg atgggatatc aaggcacgca
      121 ccaccatgaa ctggcggccg ttgtcgagtt catccacacg gcgacgctgc tgcatgacga
      181 tgtggtcgac gaatccgaac tgcgacgcgg taggcaaacc gccaatgctg cctttggcaa
      241 cgcagccagc gttctggtcg gagacttcct ctattcgcgt gctttccaga tgatggtggc
      301 agtcgacaac atgcgcgtga tgcaggtcct ttccgacgcc accaacatca tcgccgaggg
      361 cgaagtgttg cagttgatga actgccacga cgccgacgtc gatgaagagc gctacatgca
      421 ggtcatccac tacaagacgg ccaagctgtt cgaagccgcc gctcgcctcg gcggtatcat
      481 cggcgatgcg gagcccgtcg tcgaggccgc cctggcgtct tacggcatgc atctggggac
      541 ggctttccaa ctggtggacg acgtgctcga ctattccggc caggaagcgg ataccggcaa
      601 acatctcggt gacgacctgg ctgaaggcaa gccgactttg ccgctgatcc acgcgatgca
      661 gcacggcacc ccggaacacg ccgcctgtgt tcgcaaggcg atcgaggaag gtggacgcga
      721 tgcgttcccg gaagtgctcc aggctatcca gcagagtggg gctttggaac acacccgggc
      781 ccgggcggca aaggaagcgg agcaggccag agcagcaatt tcgctgctgg gggattctct
      841 ttataaaaaa gctctgctag aattgacctt cttagctgtt gagcgaaatc actaggtttc
      901 gcaaagcaca tcggagtgta gctcagcctg gtagagcact gcgttcggga cgcaggggtc
      961 gcatgttcga atcatgtcac tccgaccaaa gaatctacgg aaagccttga tttatcaagg
     1021 cttttttcgt ttctgtcggt gcgttgagca aagtgccgtc tttgtgctcc gatatcgctc
     1081 caaagtaacc cgttaccgct cgggactccg tcgtctccgg tttatccctt gccgccgacg
     1141 tcatacgggc agggctggcg ttccagattc agcaggtgat ggtcgaattc ctggatcagg
     1201 gccggtgccg tttcatcgag caggaaagcg cggaatttgg cggcggccgg catcaggcgc
     1261 ttggcgctca tgttgaccac gtaccaggtg cgttcgattg gtgtgcctac gacgtcgagc
     1321 aggcacaggg cgtgggtctt gagttccagc ggcaaggtgt gcagcgacag caggctgacg
     1381 cccatgccgg ccatcaccgc ctgcttgatg gtttcgttac tgcccatgct gatcgtccgc
     1441 gccggggtga acaggtggtt cttgaacatt tcctcggcaa cgcgccgcga gcccgagcct
     1501 tcctcgcgca gcaggaaggt ctcgtgccgc agttcgtgca tctcgaagcg gcgggcgtcg
     1561 cgcagcggat ggtcggccgg ggcgatcagg acgtaggggt ggcaggccat cggctcaccg
     1621 tgcgcgtcga tttcctgcgg aatgcggccc atgatcgcca ggtcgatggc gttgtcctgc
     1681 agcttctgca agagcgcctg gcggttgccg acgctgaact ggacatcgac gccggggtgt
     1741 tgctgggcga acttggcgag caggcgcggc atgaaatact tggcggtgct gaccagaccg
     1801 accgaaatct ggccgctttc ggcgccattc agcgcctgca ggctggcttc ggcgtccttg
     1861 atctcgccca ggatgcgtag cgcgtgatgg accagcagct caccggcgcc ggtcagcgcg
     1921 acgccgcgtc ccatgcgttc
//
LOCUS       DAOSDX010000604         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20160_length_1939_cov_3.528132, whole genome shotgun sequence.
ACCESSION   DAOSDX010000604 DAOSDX010000000
VERSION     DAOSDX010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20160_length_1939_cov_3.528132"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLN02_12090"
     CDS             complement(<1..798)
                     /locus_tag="PLN02_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOI53110.1"
                     /translation="MISRSLKQLSQALAAKEISSVELSSLFLDRIARLNPEINAFITV
                     DREKTLAMAAAADQRLAAGTGGALTGIPIAQKDIFCAEGWTTTCGSKMLANFVAPYDA
                     TVIAKMHGEAGLVSLGKTNMDEFAMGSSNESSHFGPVKNPWDRARVPGGSSGGSAAAI
                     AARLAPAATGTDTGGSIRQPAALCNLTGLKPTYGVVSRYGMIAYASSLDQGGPMAGSA
                     EDCALLLNAMAGFDVRDSTSLDRPAEDYTRDLEQPLAGLRIGLPREFF"
     gene            complement(795..1082)
                     /gene="gatC"
                     /locus_tag="PLN02_12095"
     CDS             complement(795..1082)
                     /gene="gatC"
                     /locus_tag="PLN02_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOI53111.1"
                     /translation="MSLTTEQVLRIAHLARIEVSESEALTTQAHLNGIFALIEQMQAV
                     DTRGIEPMAHAQDLGQRLRDDVVSEPDRRAAFQAVAPETEAGLYLVPKVIE"
     gene            1188..>1939
                     /locus_tag="PLN02_12100"
     CDS             1188..>1939
                     /locus_tag="PLN02_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOI53112.1"
                     /translation="MFGFLSKYFSNDLAIDLGTANTLIYVRGRSIVLDEPSVVAIRLE
                     GGPNAKKTIQAVGREAKEMLGKAPGSITVIRPMKDGVIADFTVTEQMLKQFIKKAHDS
                     KLFSPSPRIIICVPSGSTQVERRAIRESALGAGASQVYLIEEPMAAAIGAGLPVAEAT
                     GSMVVDIGGGTTEVGVISLGGMVYAGSVRVGGDKLDEAIINYISRNYGMMIGENTAEN
                     IKKNIGSAFPGAEIKEMEVSGINKAEGIPRKFT"
BASE COUNT          367 a          626 c          617 g          329 t
ORIGIN      
        1 gaagaactcc cggggcaggc cgatgcgcag gccggccagc ggttgttcga ggtcgcgcgt
       61 gtaatcctcg gccgggcggt cgagcgacgt cgaatcgcgg acatcgaaac cggccatcgc
      121 gttgagcagg agggcgcagt cttccgccga gccagccatc gggccgccct gatcgagcga
      181 cgaggcgtag gcgatcatgc cgtagcgcga gacgacgccg taggtcggct tgagaccggt
      241 caggttgcac agcgccgccg gctggcggat cgagccgccg gtatcggtgc cggtggctgc
      301 cggcgccagt cgtgcagcga tcgccgccgc cgaaccgccg gacgaaccgc cgggcacccg
      361 cgcgcgatcc cagggattct tcaccggacc gaaatgcgag ctttcgttcg acgagcccat
      421 cgcgaactcg tccatgttgg tcttgcccag cgagaccagg ccagcctcgc catgcatttt
      481 cgcgatcacc gtcgcatcgt agggcgcaac gaagttggcc agcatcttcg agccgcaggt
      541 cgtcgtccag ccttcggcgc agaagatgtc cttctgggcg atcgggatgc cggtcagcgc
      601 accaccggta cccgccgcca ggcgctggtc ggcagccgcc gccatggcca gcgtcttctc
      661 ccggtcgacg gtgatgaagg cgttgatctc gggattgagg cgggcgatgc ggtcgaggaa
      721 gagcgagctc agttcgacgc tggaaatttc cttcgccgcc agtgcctgcg acagttgttt
      781 caaactccgg ctaatcattc gatcaccttc ggcacgaggt agagaccggc ctcggtttcc
      841 ggggcgacgg cctgaaaggc ggcgcgccgg tcgggctcgc tgacgacgtc atcgcgcagg
      901 cgctggccga gatcctgagc gtgggccatc ggctcgatac cgcgggtatc gaccgcctgc
      961 atctgttcga tcagcgcgaa gatgccgttg aggtgggctt gcgtggtcag tgcctcggat
     1021 tcgctgacct cgatgcgggc gagatgggca atccgcagaa cctgttctgt agtgagcgac
     1081 atggcttgaa aaaggctttt tgcagcgcac aaagccttat aaacgaaagc gttgtaaggt
     1141 atcataaaag ctttatctac cgcacccccc aacgcggagc tcaacagatg ttcggattcc
     1201 tctccaagta tttttccaac gaccttgcca tcgacctcgg cacggccaat acgctgattt
     1261 acgtgcgtgg ccgctcgatc gtcctcgatg agccgtcggt cgttgcgatc cgcctcgaag
     1321 gcggccccaa cgccaagaaa accatccagg ccgtcggccg cgaagccaag gaaatgctgg
     1381 gcaaggcgcc cggcagcatc accgtcatcc ggccaatgaa ggatggcgtg atcgccgact
     1441 tcacggtcac cgagcagatg ctcaagcagt tcatcaagaa ggcgcacgat tccaagctgt
     1501 tctcgcccag cccgcgcatc atcatctgcg tgccgtccgg ctcgacccag gtcgaacgcc
     1561 gcgccatccg cgagtcggcg ctcggcgccg gcgccagtca ggtgtatctg atcgaggaac
     1621 cgatggcggc cgccatcggt gccggcctgc cggtcgccga agcgaccggc tcgatggtcg
     1681 tcgacatcgg cggcggcacc accgaagtcg gcgtcatctc gctcggcggc atggtctacg
     1741 ccggctccgt gcgcgtcggc ggcgacaagc tcgacgaggc gatcatcaac tacatcagcc
     1801 gcaactacgg catgatgatc ggcgaaaaca ccgccgagaa catcaagaag aacatcggct
     1861 ccgctttccc gggcgccgag atcaaggaaa tggaagtctc cggcatcaac aaggccgaag
     1921 gcatcccgcg caagttcac
//
LOCUS       DAOSDX010000605         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20162_length_1939_cov_3.139066, whole genome shotgun sequence.
ACCESSION   DAOSDX010000605 DAOSDX010000000
VERSION     DAOSDX010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20162_length_1939_cov_3.139066"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLN02_12105"
     CDS             <1..258
                     /locus_tag="PLN02_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285965.1"
                     /note="SAM-dependent;catalyzes the methylation of cytosine
                     at position 1962 of the 23S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HOI53113.1"
                     /translation="ERAARAYKDINVLGCRLLRPGGMLMTYSCSGGIGLELFQKIVAG
                     AAHDAGREARIVRRLAGAADHPVALNFPEGEYLKGLLVQVD"
     gene            complement(312..629)
                     /locus_tag="PLN02_12110"
     CDS             complement(312..629)
                     /locus_tag="PLN02_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53114.1"
                     /translation="MNSPSKSSSADIANGTRPGGGADATGGLAAGAGGAAFAGGQAST
                     ENSRSGNGFSSFCGSPACGGGTFISGSGARGLATVALGTTFSGCFLKKEKTMSTPHAE
                     QLF"
     gene            595..1635
                     /locus_tag="PLN02_12115"
     CDS             595..1635
                     /locus_tag="PLN02_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI53115.1"
                     /translation="MSAEDDFDGEFTASSVMAIDVVHDIDPLQADVEQVAVLFANGQD
                     DAARALLLSVLQTYSGSEGIRLWRMLFDLLQIQGDRSAFDRYGMEFARSCEMSPPAWR
                     DASGISAGTASTAKGFTLQGVLAGDDSDALRQLDESLQQVGDLLVDCSRLVGCDDIAA
                     GRLADALIAARRAGKVVTLQGADIFARRLEERLKTGEAVHESAWRLLLELLQRLGTQV
                     HFEEKAVDYAITFELSPPSWETLPKDVVAKQTALAAAPKDEVYYLAGDLKGLRFEELL
                     PLLEAGAPAIIDFSAVRRVDFYSAGQLVNRLAPFKAAGQDIVIRSPNHLVAELMAVVG
                     LNKQARIIVPKS"
     gene            1649..>1939
                     /gene="hslV"
                     /locus_tag="PLN02_12120"
     CDS             1649..>1939
                     /gene="hslV"
                     /locus_tag="PLN02_12120"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOI53116.1"
                     /translation="MEQYHGTTILSVRRGNVVTMGGDGQVTLGNIVIKGTAKKVRRLY
                     QGKILAGFAGGTADAFTLFERFEAKLEKHQGNLVRSAVELAKDWRTDRALRRL"
BASE COUNT          334 a          585 c          645 g          375 t
ORIGIN      
        1 gagcgtgccg cccgcgccta caaggacatc aacgtcctcg gctgccgcct gctgcgcccg
       61 ggcggaatgt tgatgaccta ctcctgttcc ggcggcatcg gcctcgaact gttccagaag
      121 atcgtcgccg gcgccgccca cgatgccgga cgcgaagcgc gtatcgtccg ccgtctggcg
      181 ggggcggcag accacccggt ggcgctgaat tttcccgagg gtgagtactt gaaaggcctg
      241 ctggtacagg tcgattgagc ggggaacttg cggactttcc ttaaaacatt gccatgagcg
      301 gtccgggcat gctaaaaaag ctgctcagcg tgcggagtgc tcatggtttt ctcttttttc
      361 aagaaacagc ccgaaaaagt ggtgcccaag gccacggtgg ccaggccgcg cgctccgctg
      421 ccggaaatga aggtgccgcc gccgcaggcc ggcgagccac agaagcttga aaagccgttg
      481 ccggatctcg agttctccgt agaggcctgc cccccggcaa aggctgctcc tcctgcaccg
      541 gcggcaaggc cgccagtggc gtcagcaccg ccgccgggcc gggtgccgtt ggcgatgtcg
      601 gcggaagatg atttcgacgg cgaattcacc gcctcgtcgg tgatggcgat cgacgtggtg
      661 cacgatatcg atcccctgca ggccgacgtc gagcaggtgg cggttctttt tgccaacggc
      721 caggacgatg ctgcgcgcgc gctgctgttg agcgttttgc agacctattc gggcagcgaa
      781 ggcatccgcc tgtggcggat gttgttcgat ctcttgcaga ttcaggggga tcgcagcgct
      841 ttcgaccgtt acggcatgga gttcgcgcgc agctgcgaaa tgtcgccgcc ggcttggcgg
      901 gacgcttccg gaatcagcgc cgggacggcg tcgactgcga agggtttcac gctgcagggc
      961 gtgctggccg gcgacgacag tgatgcgttg cggcaacttg acgaatcctt gcagcaggtt
     1021 ggcgacctac tggtcgattg cagccgcctg gtcggttgtg acgacattgc tgccggccgt
     1081 ctggccgatg cgctgattgc cgcccggcgt gccggcaagg tggtgacctt gcagggtgcc
     1141 gacatcttcg cgcgccgcct cgaagagcgc ctgaaaaccg gcgaggccgt gcacgaatcg
     1201 gcgtggcgcc tgctgctgga gttgttgcag cggcttggta cgcaagtcca tttcgaggaa
     1261 aaggcggtcg attacgcgat taccttcgag ctttctccac cgtcgtggga aacgctgccg
     1321 aaggatgtcg tggccaagca gacggcgctt gccgccgcgc cgaaggatga ggtgtattac
     1381 ctggccggcg atctcaaggg gttgcgtttc gaggaactgt tgcccttgct cgaagcgggc
     1441 gctccggcga tcatcgattt ctccgcggtc cgtcgcgtcg atttctattc ggccgggcaa
     1501 ctggtcaatc ggctggcgcc attcaaggcc gccggccagg atatcgtgat ccgcagtccg
     1561 aaccatctcg ttgccgagtt gatggccgtg gtcggcctca acaagcaggc gcgcatcatc
     1621 gttcccaagt cctaaaaaag gttatcccat ggagcaatat cacggcacga cgatactgtc
     1681 ggtgcgccgg ggcaatgtcg tcacgatggg tggcgacggc caggtgacgc tgggcaacat
     1741 cgtcatcaag ggcacggcga aaaaggtccg tcgtctctat cagggcaaga tcctcgccgg
     1801 ctttgccggc ggcactgccg atgccttcac gctgttcgag cgcttcgagg ccaagctgga
     1861 aaaacatcag ggcaatctgg tccgttccgc cgtcgagctg gccaaggact ggcgcaccga
     1921 ccgtgccctg cgccggctg
//
LOCUS       DAOSDX010000606         1934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20215_length_1934_cov_3.309739, whole genome shotgun sequence.
ACCESSION   DAOSDX010000606 DAOSDX010000000
VERSION     DAOSDX010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20215_length_1934_cov_3.309739"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLN02_12125"
     CDS             complement(<1..865)
                     /locus_tag="PLN02_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOI53117.1"
                     /translation="MRIQIKHKLLILVAVAVLALLGVSLFALGQATKLHDELEESIAR
                     SATMIEAIDGARSAQVSFKIMVQEWKNTLLRGKDPEAYAKHLKSFDAEAARVSEQLTK
                     VGQTAAGLGVAERLKIEQVTGTFGQLAPRYHEALGQYDRNAADPAATVDKLVRGIDRE
                     PTKVIDDLVDEMQKISTELQASDRERTDEVYSSVRLGLGVFAVAALIVLLTLALLFIR
                     SIIGPLARLESTMSHISGSGDLTRRAAIVNEDEIGRMSRAFNAMMERLQQLIREINTA
                     SGEVASASEQLA"
     gene            1111..>1934
                     /locus_tag="PLN02_12130"
     CDS             1111..>1934
                     /locus_tag="PLN02_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOI53118.1"
                     /translation="MTTISTLFLLAGLLLFISVLASTVSARLGLPLLLLFLSVGMLAG
                     EDGPGGILFSDFFTANFVGQLALAVILLDGGLRTDAATFRVALKPAAVLATWGVIGTV
                     ALLGVFATWVLDVDWRLGLLLAAIVGSTDAAAVFALLRHSGVRLNERVKSTLEIESGS
                     NDPMAILLVTVLVEILLHPERAGVAAFAVLLLKQAGIGLLFGLVGGKILAALLRRLNL
                     AEGLYALLILSGGLLIVGAANLLDGSGLLAVYIAGVIVGNRRSHATEHVLRVMDGLA"
BASE COUNT          292 a          594 c          614 g          434 t
ORIGIN      
        1 gcgccagttg ttcggaggcg ctggcaactt cgccgctggc cgtgttgatt tcgcggatca
       61 gttgctgcag gcgttccatc attgcgttga aggcgcgcga catgcggccg atttcatcct
      121 cgttgacgat cgccgcgcgg cgcgtcaggt cgccgctgcc ggaaatgtgg ctcatcgtcg
      181 attcgagcct ggccagcggt ccgatgatcg agcggatgaa gagcaaggcc agcgtcagca
      241 ggacaatcag ggcggcgacg gcaaagacgc cgagccccag tctgaccgag gaatagactt
      301 cgtcggtgcg ctcgcggtcg ctggcctgca gttcggtcga gattttctgc atctcgtcga
      361 ccagatcgtc gatcaccttg gtcggttcac ggtcgatgcc gcgtaccagt ttgtcgactg
      421 tagcggccgg gtcggcggcg ttgcggtcat attgaccgag agcttcatgg taacgcggcg
      481 ccaactggcc gaaggtgccg gtgacctgtt cgatcttcag gcgttcggcg acgccaagac
      541 ccgccgcggt ttggccgacc ttggtcaact gttcgctgac ccgggcggct tcggcatcga
      601 agcttttcag gtgcttggcg taggcttccg gatccttgcc gcgcaacagg gtgttcttcc
      661 actcctggac catgatcttg aagctgacct gggcgctgcg ggcgccgtcg atggcttcga
      721 tcatcgtcgc gctgcgggcg atcgattctt cgagttcatc gtgcagcttg gtggcctgac
      781 ccagcgcgaa gagactgaca ccaagcaggg caagtacggc gacggcgacg aggatcaaca
      841 gcttgtgctt gatttggatg cgcatcgcaa tactcctcct gtgtgcgaat ttgctgaatt
      901 gtacagacgt tgacggctgg ttgttggttc aatcgatttt gataaatttg tgttccggct
      961 tcgcttcgcc gcgcgctgtt gcttctttgc cgtgcggtga cggcgtcggg atatagacca
     1021 cggtgcctcc gatagattgc cgctgccgca caaaatcctg gtaaccaaaa cgcgacaaaa
     1081 tcgccgatac ccttgtgata aattcggact atgacaacga tcagcaccct gttcctgctg
     1141 gccggcctgc tgctcttcat cagtgttctt gccagcaccg tttcggcccg ccttggcttg
     1201 ccactgctgc tcctttttct cagcgtcggc atgctggctg gcgaggatgg gccgggcggc
     1261 atcctgttca gcgatttctt caccgccaac ttcgtcggcc aactggcgct ggcggtgatc
     1321 ctgctcgacg gcggcttgcg caccgatgcc gcaaccttcc gcgtagccct gaagccggcc
     1381 gccgtgctcg ccacctgggg ggtcatcggt accgtcgccc tgcttggcgt gtttgccacc
     1441 tgggtactcg acgtcgattg gcggctcggc ctgctgctcg ccgccatcgt cggctcgacc
     1501 gatgcggcgg cggtgttcgc cctgctgcgt catagcggcg tgcgtttgaa cgagcgggtc
     1561 aaaagcacgc tggaaatcga atccggttcc aacgatccga tggccatcct gttggtcacc
     1621 gtgctggtcg aaatcctgct gcatcccgag cgcgccgggg tcgccgcgtt tgccgtgctg
     1681 ttgctgaaac aggccgggat cggcctgctc ttcggcctgg tcggcggcaa gattctggct
     1741 gccctgttgc gccgattgaa cctggccgaa ggcctgtatg cgctgctgat cctctccggc
     1801 ggcctgctca tcgtcggcgc cgccaacctg ctcgacggca gcggcctgct cgccgtctac
     1861 atcgccgggg tgattgtcgg caaccggcgc agtcacgcca ccgagcacgt gttgcgggtg
     1921 atggacgggc tcgc
//
LOCUS       DAOSDX010000607         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20239_length_1933_cov_2.370075, whole genome shotgun sequence.
ACCESSION   DAOSDX010000607 DAOSDX010000000
VERSION     DAOSDX010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20239_length_1933_cov_2.370075"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLN02_12135"
     CDS             complement(<1..617)
                     /locus_tag="PLN02_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOI53119.1"
                     /translation="MNKLIVKKAAVLGAGVMGAQIAAHLANAGVPVVLFDLAAKEGPK
                     NGIVNKALDGLKKLDPAPLASRDKLQLIDAANYDEHLPLLAGCDLVIEAIAERMDWKN
                     DLYAKIAPHLSATAIIASNTSGLSMAALAQGLPENVQPRFCGIHFFNPPRYMHLVEVI
                     ATPGTDAATLDALETWLTSRLGKGVIRALDTPNFVANRIGVFSILA"
     gene            complement(774..>1933)
                     /locus_tag="PLN02_12140"
     CDS             complement(774..>1933)
                     /locus_tag="PLN02_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOI53120.1"
                     /translation="GFQLIEQNASGIGNAYAGSAAVAENASTIFYNPAGMTQLQAREF
                     SVGLSLVRPSFNFSNKGSVVPALGPVSLSGDGGDGGSWAALPNAYMSWALNKDVYLGV
                     GLGAPFGLMTEYDKNWLGAAHSIKFDIKTYNINPSIAWRVNDKVSLGAGINYMLMDVE
                     YIKRAGAATVAPIAKLQADDTGWGWNIGALFTLSESTKLGLSYRSKVKQKLDGDLTVV
                     GVTQVDAQAKLELPDTAIVSLTHKLNDKWELLGDVSWTGWSSIPKVVITNALGNQTLD
                     TKFDDSWRVALGANYALNPTTKLKFGVAYDQTPVPDAEHRLVSLPDNDRLWLSLGAQW
                     KPSKATTVDLGASYLFVKDTKINNNGGASGLIRGDYDSDVWILGGQFSMAF"
BASE COUNT          356 a          583 c          617 g          377 t
ORIGIN      
        1 gccaggatgg aaaagacgcc gatgcggttg gcgacgaagt tcggcgtatc gagcgcgcgg
       61 atgacgccct tgcccaggcg cgacgtcagc caggtttcca gcgcgtcgag ggtggcggcg
      121 tcggtgcccg gggtggcgat cacctcgacc agatgcatgt agcgcggcgg gttgaagaaa
      181 tggataccgc agaagcgcgg ctgcacattt tccggcaacc cctgcgccag ggccgccatc
      241 gacagcccgg aggtgttgga cgcgataatg gcggtggccg acaggtgcgg cgcgatcttc
      301 gcgtagaggt cgttcttcca gtccatgcgc tcggcgatgg cctcgatcac cagatcgcag
      361 ccggcgagca gcggcaggtg ctcgtcgtag ttggcggcgt cgatcagttg cagcttgtcg
      421 cggctggcca gcggcgccgg atcgagtttc ttcaggccat cgagcgcctt gttgacgatg
      481 ccgttcttcg ggccttcctt ggcggccaga tcgaacagca cgaccggcac gccggcgttg
      541 gcgaggtgcg ccgcaatctg cgcccccatc accccggcgc cgagcacggc agccttcttg
      601 acgatcagct tgttcaaagc ttgtctcctt tcgtggtctt atcgttgtcg taaaaataaa
      661 acgaacgttt gaattataga catcgaagcc tgacggtcaa tgccttggcg cggaaaaact
      721 gtaaaaaaat ccccgggaca tgcccgggga ttttccattg tgctcacgta gcctcagaaa
      781 gccatcgaga actgcccgcc gaggatccag acatcgctgt cgtagtcgcc gcggatcaga
      841 ccgctggcgc caccgttgtt gttgatcttg gtatccttga cgaagagata ggaggcaccg
      901 agatcgaccg tcgttgcctt gctcggcttc cattgcgcac cgagcgacag ccacaggcgg
      961 tcattgtcgg gcagcgagac caggcgatgt tcggcatccg gcaccggcgt ctggtcgtag
     1021 gcgacgccga acttcagctt ggtcgtcggg ttcagcgcat agttggcacc gagcgcgacg
     1081 cgccagctat cgtcgaactt ggtgtcgagc gtctggttgc ccagcgcatt ggtgatcacg
     1141 actttcggga tgctgctcca gccggtccac gacacatcgc cgagcagttc ccacttgtcg
     1201 ttcagcttgt gcgtcaggct gacgatggcg gtatcgggca attccagctt ggcctgggca
     1261 tcgacctggg tcacgccgac gacggtcagg tcgccgtcca gcttctgctt gaccttggaa
     1321 cgataggaga gacccagctt ggtcgactcg gagagcgtga acaaggcgcc gatgttccag
     1381 ccccaacccg tgtcgtccgc ctgcagcttg gctatcggcg cgacggtggc cgctccggca
     1441 cgcttgatgt actcgacatc catcagcatg tagttgatgc cggcaccgag cgaaaccttg
     1501 tcgttgacgc gccaggcgat cgacggattg atgttgtagg tcttgatgtc gaacttgatc
     1561 gagtgcgccg cccccagcca gttcttgtcg tactcggtca tcaggccgaa gggcgcaccc
     1621 agaccgacac cgagatagac atccttgttc agcgcccagg acatgtaggc attcggcagc
     1681 gctgcccagc taccgccgtc gccaccatcg ccggaaagcg acaccggacc gagagcggga
     1741 acgaccgagc ccttgttgct gaagttgaag ctcggccgga ccagcgacag accgaccgag
     1801 aattcgcgcg cctgcagttg ggtcatgccg gcggggttgt agaagatggt gctggcgttc
     1861 tcggcaacag cggccgaacc ggcgtaggcg ttaccgatcc cgctggcgtt ctgttcgatc
     1921 aactggaaac cgg
//
LOCUS       DAOSDX010000608         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20247_length_1932_cov_2.864678, whole genome shotgun sequence.
ACCESSION   DAOSDX010000608 DAOSDX010000000
VERSION     DAOSDX010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20247_length_1932_cov_2.864678"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLN02_12145"
     CDS             complement(<1..396)
                     /locus_tag="PLN02_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOI53121.1"
                     /translation="MSQPTPPSTTLASPDDVEHAFYDALASGDADLLMHCWSEEDESV
                     CVHPTGARLTGVPLIRESWRAIFASTRLRIHSETVARWQGSVMAVHHLSETLFVGDDP
                     APHGPLHVTHVYVRGAHGWRLACRHASAAD"
     gene            complement(393..1406)
                     /gene="waaF"
                     /locus_tag="PLN02_12150"
     CDS             complement(393..1406)
                     /gene="waaF"
                     /locus_tag="PLN02_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOI53122.1"
                     /translation="MNKALIVAPSWIGDTIMAQPLFARLRERHPGLHLDALAPRWVAP
                     VLRRMAEIDDIVDNPFAHGELSLKARWRLGRELAGRGYDAAWVLPNSLKSALLPLFAG
                     IPRRVGFTGEARYGLINVRHHLDEAALPLMVERFAQLAAAPGGALPRPIPHPRLRSTD
                     DDRQRTLSELAMVRPDKLVAFCPGAEYGPAKRWPAAHFAELARNLAAAGYVVWLFGSP
                     KDHGVAEEIAALAPGCCRNLCGATRLDQAVDLLALADFVVCNDSGLMHVAAAVDRPIV
                     ALYGSSSPGFTPPLSERADIVSLQLDCSPCFKRDCPLGHLDCLNRLLPAQVFAACQKW
                     MQA"
     gene            complement(1443..1631)
                     /locus_tag="PLN02_12155"
     CDS             complement(1443..1631)
                     /locus_tag="PLN02_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HOI53123.1"
                     /translation="MSDHKTVEVSAADLPLHCPQPGSALWSQHPRVFLDVLKTGEAVC
                     PYCGTRYTFAGEAPKGHH"
     gene            complement(1653..>1932)
                     /locus_tag="PLN02_12160"
     CDS             complement(1653..>1932)
                     /locus_tag="PLN02_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOI53124.1"
                     /translation="ACLEGITRATVMQLAGELGLEVIEKRISRDEVYCADEAFFTGTA
                     AEVTPIRELDNRQIGPGHRGPITKALQEKYFDVVYGRSAAHADWLAHV"
BASE COUNT          292 a          601 c          741 g          298 t
ORIGIN      
        1 gtcggcggcc gaggcgtggc ggcaggcgag ccgccagccg tgggcgccgc gcacatagac
       61 atgggtgacg tggagcggcc cgtgcggcgc cggatcgtcg ccgacgaaca gggtttcgct
      121 caggtgatgc accgccatca ccgatccctg ccagcgggcg acggtttcgc tgtggatgcg
      181 caaccgggtg ctggcaaaga tcgcccgcca gctttcgcgg atcagcggta cgccggtcag
      241 ccgggcgccg gtcgggtgga cgcagacgct ttcatcctcc tcgctccagc aatgcatcag
      301 caggtcggca tcgccgctgg ccagcgcgtc gtagaaggcg tgctcgacgt cgtccggcga
      361 ggccagggtg gtggaaggcg gggtgggctg gctcatgctt gcatccattt ctgacaggcg
      421 gcgaaaacct gcgccggcag cagccggttc aggcagtcga ggtggccgag cgggcagtcg
      481 cgcttgaagc acggactgca gtcgagttgc aggctgacga tgtcggcgcg ctcggagagc
      541 ggcggcgtga acccgggcga cgacgagccg tagagggcga cgatcgggcg gtcgacggcg
      601 gcggcgacgt gcatcagccc ggagtcgttg cagacgacga agtcggccag tgccagcagg
      661 tccaccgcct ggtcgaggcg ggtggcgccg cacaggttgc ggcagcaccc gggggcgagg
      721 gcggcgatct cctcggcaac accatggtcc ttcggcgagc cgaacagcca gacgacgtag
      781 ccggcggcgg ccaggttccg cgccagttcg gcgaaatgcg ccgccggcca acgcttcgcc
      841 gggccgtatt cggcgcccgg gcagaaggcg accagcttgt ccgggcggac catggcgagt
      901 tcggagagcg tccgctgccg gtcgtcgtcg gtcgagcgca gccgcggatg cgggatcggc
      961 cgtggcagcg cgccaccggg cgctgcggcg agttgtgcga agcgctcgac catcagcggc
     1021 aatgcagctt catcgagatg gtggcggacg ttgatcaggc cgtagcgtgc ttcgccggtg
     1081 aagccgacgc gtcggggaat gccggcaaag agcggcagca gtgccgattt cagcgagttg
     1141 ggcaggaccc aggcggcgtc gtagccgcgc ccggcaagct cgcggccgag gcgccagcgc
     1201 gccttgagcg agagttcgcc gtgggcgaag gggttgtcga cgatgtcgtc gatctccgcc
     1261 atgcgccgca gtaccggtgc cacccagcgt ggggcgaggg cgtcgaggtg cagtccggga
     1321 tggcgttcgc gcaggcgcgc gaacagcggc tgcgccatga tggtgtcgcc gatccaggac
     1381 ggcgcgacga tcagggcctt gttcatggaa aaacggcggg tttcacaccc gccgcctctg
     1441 gctcagtgat ggcccttggg cgcttcgccc gcgaaggtgt agcgggtgcc gcagtaggga
     1501 caaaccgctt cgccggtctt cagcacgtcg aggaagacgc gcgggtgctg gctccacagc
     1561 gcggaacccg gctgcgggca gtgcagcggc aggtcggcgg cgctgacttc gacggtcttg
     1621 tggtcggaca tcgttctctc cctgagcttg gatcagacgt gggccagcca gtcggcgtgc
     1681 gccgccgagc ggccatagac cacatcgaaa tatttttcct gcagcgcctt ggtgatcggg
     1741 ccgcggtggc cggggccgat ctgccggttg tcgagttcgc ggatcggcgt cacttcggcg
     1801 gcggtgccgg tgaagaaggc ttcgtcggcg cagtagactt cgtcgcggct gatgcgcttc
     1861 tcgatcacct cgaggcccag ttcgccggcc agttgcatga ccgtggcgcg ggtgatgcct
     1921 tccaggcagg cg
//
LOCUS       DAOSDX010000609         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20276_length_1931_cov_2.575160, whole genome shotgun sequence.
ACCESSION   DAOSDX010000609 DAOSDX010000000
VERSION     DAOSDX010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20276_length_1931_cov_2.575160"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLN02_12165"
     CDS             complement(<1..546)
                     /locus_tag="PLN02_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HOI53125.1"
                     /translation="MSGWRDEPRAFLRRLFDAGLAAADPARCVPPALAAIDPPGPGGR
                     LIVVGAGKASAAMAKAVEEHWPGPLSGLVVTRYGHGVPCSRIEIVEAAHPVPDAAGEA
                     AAARILALLSGLSANDRVLALISGGGSALLVAPAPGVTLAQKRALTDALLRSGAPINE
                     INCVRKQLSAIKGGRLALAAWP"
     gene            complement(543..1316)
                     /gene="hyi"
                     /locus_tag="PLN02_12170"
     CDS             complement(543..1316)
                     /gene="hyi"
                     /locus_tag="PLN02_12170"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HOI53126.1"
                     /translation="MPKFAANLSFLFADVPFPARFARAAAAGFAGVEYLFPYAWPAAG
                     IASWLRDAGLQQVLFNLPPGDWDAGERGIACLPGREKEFAAGVDTALVYAQALDCRQV
                     HCMAGLRPAGADDAALTATYVANLRHAADRFAGISATVLIEPINSRIDMPGYWLDDIR
                     HAVALLDAIDRPNVRLQLDLYHAQIIQGDLARCIEANIERIGHLQIADNPGRHEPGSG
                     EINYPFLFALLDRLAYAGWVGCEYKPLTTTEAGLGWLPR"
     gene            1380..>1931
                     /locus_tag="PLN02_12175"
     CDS             1380..>1931
                     /locus_tag="PLN02_12175"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOI53127.1"
                     /translation="MDNEKERKGNRLSKIVTRTGDAGTTGLGDGSRTTKDSLRIAAIG
                     EVDELNSSLGVLLCEELPDAVRAALLDIQHDLFDLGGELCLPGMAVMKDAQVERLEAL
                     AEQLNGELPMLKEFILPGGTRPAALAHLSRTVCRRAERAMVALHHAETLSDAARRYIN
                     RLSDLLFVFGRALNRAGGRGDVLW"
BASE COUNT          318 a          675 c          683 g          255 t
ORIGIN      
        1 cggccaggcg gcgagcgcca ggcgcccgcc cttgatcgcc gagagctgct tgcgcacgca
       61 gttgatttcg ttgatcggcg cgcccgagcg caacagggcg tcagtgagcg cccgcttctg
      121 cgccagcgtg acacccggcg ccggtgccac cagcagcgcc gaaccgccgc cggaaatcag
      181 cgccagcacg cggtcgttgg cgctcagccc ggagagcaga gcgagaatgc gcgccgctgc
      241 cgcctcgccg gcggcatccg gcaccgggtg cgcggcctcg acgatttcga tgcgcgagca
      301 gggcacgccg tgcccgtagc gggtgacgac cagcccggac agcgggccgg gccagtgttc
      361 ctcgaccgcc ttggccatcg ccgccgacgc cttgccggcg ccgacgacga tcaacctgcc
      421 gcccggaccc ggcgggtcga tggcggccag cgccggcggc acacagcgcg ctgggtcggc
      481 cgccgccaac ccggcgtcga acagacggcg caggaaggcg cgcggctcgt cgcgccagcc
      541 gctcatcgcg gcaaccagcc gaggccggcc tcggtcgtcg tcaacggctt gtattcgcag
      601 ccgacccagc cggcgtaagc aagccggtcg agcagcgcga acaggaaggg ataattgatc
      661 tcgccgctgc ccggctcgtg acgccccggg ttgtcggcga tctggaggtg gccgatacgc
      721 tcgatgttgg cctcgatgca gcgcgccagg tcgccctgga tgatctgcgc gtggtagagg
      781 tcgagttgca ggcggacgtt ggggcggtcg atcgcatcga gcagcgcgac cgcgtggcgg
      841 atgtcgtcga gccagtaacc gggcatgtcg atacggctgt tgatcggttc gatcagcacc
      901 gtcgcgctga tgccggcgaa gcggtcggcg gcatggcgca ggttggcgac gtaggtggcg
      961 gtgagcgcgg catcgtcggc gccggccggg cgcaagccgg ccatgcagtg cacctgccgg
     1021 caatcgagcg cctgcgcgta aaccagcgcc gtgtcgacgc cggcggcgaa ttccttttcg
     1081 cgccccggca ggcaggcgat accccgttcg ccggcgtccc agtcaccggg cggcaggttg
     1141 aacagtacct gctgcaagcc ggcgtcgcgc agccagctgg caatcccggc ggccggccag
     1201 gcatagggaa agaggtactc gacgccggcg aaaccggccg ccgcggcgcg cgcgaagcgc
     1261 gctgggaaag ggacgtcggc gaacaggaaa ctgaggttgg cggcgaactt tggcatggcg
     1321 gcgacagtct ttacgaggcc cgaaagtata attcgccgtt caccaaggag aaggcaaacg
     1381 tggataacga aaaagaacgc aaaggcaatc gcctgtcgaa gatcgtcacc cgcaccggcg
     1441 acgccggcac caccgggctc ggcgacggca gccggacgac caaggacagc ctgcgcatcg
     1501 ccgccatcgg cgaggtcgac gaactcaact ccagcctcgg cgtgctgctc tgcgaggaac
     1561 tgccggacgc cgtgcgcgca gcgctactcg acatccagca cgacctcttc gacctcggtg
     1621 gcgaactctg cctgcccggc atggcggtca tgaaggacgc ccaggtcgag cgcctggaag
     1681 cgctcgccga acagttgaac ggcgagttgc cgatgctcaa ggagttcatc ctgcccggcg
     1741 gcacccggcc ggcggcgctc gcccacctca gccgcaccgt ctgccgccgc gccgagcggg
     1801 cgatggtcgc cctgcaccac gccgaaacgc tgtccgatgc cgcccgccgc tacatcaacc
     1861 ggctgtccga cctgctgttc gtcttcgggc gcgccctcaa ccgggccggc gggcgcggcg
     1921 acgtgctctg g
//
LOCUS       DAOSDX010000610         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20305_length_1928_cov_3.606514, whole genome shotgun sequence.
ACCESSION   DAOSDX010000610 DAOSDX010000000
VERSION     DAOSDX010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20305_length_1928_cov_3.606514"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /gene="fliF"
                     /locus_tag="PLN02_12180"
     CDS             complement(<1..921)
                     /gene="fliF"
                     /locus_tag="PLN02_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOI53128.1"
                     /translation="MAATTDTTAGNAPPENQVQRLREAFNRLSERQKIGLAVAIATIV
                     ALIVGSILWSRQPDYKVLFSNLDERDGGNIVASLEQQNIPHRMSSTGAIMVPAERVHE
                     VRLKLASQGLPRGGMIGFELMENQKFGISQFAEQVNYQRGLEGELARTIMSIGSVQAA
                     RVHLAIPKPSVFVREEQKPTASVMLSLYPGRTLDSAQVAGITHLVSSSVPQLQPGNVS
                     ILDQNGSLLSTLKNKLTEAGLDATQLKYVREVENGIVARIEDILKPVLGPDNFKVQVA
                     ADIDFSQSEQTAETYRPNATPETTAIRSRQN"
     gene            1104..>1928
                     /locus_tag="PLN02_12185"
     CDS             1104..>1928
                     /locus_tag="PLN02_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOI53129.1"
                     /translation="MFNQVSAELTQAYEALQSRVTSLTEELAVANGELRRQYREKEAL
                     SERLSSLLDALPAGVVLLDQGARVTAVNPAAIAMFGEAVVGQHWGDVVSQSLAPTLTV
                     GEWLLGEARVSIAESTLPSGDGKLLLIHDITSAQRMKEELERSQRLAAMGEMAASLAH
                     QLRTPLAAALLHASNLGQPGISDEARSRFSEKATGQLRRLERLIQDVLLFARGESIGH
                     DIIPAGELLADAAQTMDALMREHGIEFVLVDHCGDAKIVGSRKALFGALVNLLENAL"
BASE COUNT          327 a          576 c          657 g          368 t
ORIGIN      
        1 gttctgccgg ctgcggatcg cggtcgtctc cggtgtcgcg ttcgggcggt aggtttcggc
       61 ggtctgttcg ctttgcgaga agtcgatgtc ggccgctacc tgcaccttga agttgtcggg
      121 gccgaggacc ggcttcagga tgtcctcgat gcgggcgacg atgccgttct cgacttcccg
      181 gacatacttc aattgggtgg cgtcgagtcc ggcttcggtc agcttgttct tcagcgtcga
      241 cagcaggctg ccgttctggt cgaggatgct gacgttgccc ggctgcagtt gcggcacgct
      301 cgacgagacg agatgcgtga tgccggcgac ctgggcgctg tcgagggtgc ggccagggta
      361 gaggctgagc atcaccgagg cggttggctt ctgctcctcg cggacgaaaa ccgagggctt
      421 ggggatggcc aggtgcacgc gtgccgcctg caccgagccg atcgacatga tggtgcgcgc
      481 cagttcgcct tccagtccgc gttggtaatt gacctgctcg gcaaactggc tgatgccgaa
      541 cttctggttt tccatcagct cgaagccgat catgccgccg cgcggcaggc cttgcgaggc
      601 gagcttcagg cgcacttcgt gcacgcgctc ggccggcacc atgatggcgc cggtgctcga
      661 catgcggtgc gggatgttct gctgttccag cgaggcgaca atgttgccgc cgtcgcgctc
      721 gtcgaggttg gagaagagaa ccttgtaatc cggctgccgg ctccacagga tggagccgac
      781 gatcagcgcg acgatcgtcg cgatggcgac ggccaacccg atcttctgcc gctcgctgag
      841 gcggttgaag gcctcgcgca ggcgctgcac ctggttttca gggggtgcat ttcctgcggt
      901 ggtatcggtg gttgctgcca tcaagtgccg ggtcgatcaa agagaaacag aagaagaatc
      961 aatcgggaag ccaaattttc ctagaaatat tctactatta ccggcgccga atgcacccga
     1021 tcattttaat gacaaaccct ccaagccagg ccgccgaacc cgttgcggcc gataaagccg
     1081 ccgaattgca gcgggcattc gccatgttca accaggtctc ggcggagctg acccaggcct
     1141 atgaagccct gcagtcgcgg gtgacttcgc tgaccgagga actggccgtt gccaacggcg
     1201 aattgcgtcg ccagtaccgg gaaaaggagg ctttgtcgga gcgcttgtcc tcgctgctcg
     1261 acgccctgcc ggcgggggtc gtgctgctcg accagggcgc ccgggtcacc gcggtcaatc
     1321 cagccgccat cgccatgttc ggcgaggcgg tggtggggca gcactggggc gacgtggtca
     1381 gccagtcgct ggcgccgacg ctgaccgtcg gtgaatggct gctcggcgag gcgcgcgtgt
     1441 cgatcgccga aagtacgctg ccttccggcg acggcaagtt gctgctgatc cacgacatca
     1501 cctcggcgca gcggatgaag gaagagctgg aacgcagcca gcgtctggcg gcaatgggcg
     1561 aaatggcggc atcgctggcg caccagttgc gcacgccgct ggcggcggcg ctgctccatg
     1621 cttccaacct gggccagccg gggatcagcg acgaggcgcg cagccgtttt tcggagaagg
     1681 cgaccggcca gttgcgacga cttgaacggc tgatccagga tgtcctgctt ttcgcgcgcg
     1741 gcgagagcat cggtcacgac atcatcccgg ccggcgaact gctggcggac gcggcgcaga
     1801 ccatggacgc cctgatgcgc gaacatggca tcgaatttgt attggtcgat cactgtggcg
     1861 atgcgaaaat cgtcggcagc cgcaaggcgc tgttcggcgc cctggtcaac ctgctggaaa
     1921 atgcactg
//
LOCUS       DAOSDX010000611         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20372_length_1923_cov_2.888116, whole genome shotgun sequence.
ACCESSION   DAOSDX010000611 DAOSDX010000000
VERSION     DAOSDX010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20372_length_1923_cov_2.888116"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLN02_12190"
     CDS             <1..481
                     /locus_tag="PLN02_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HOI53130.1"
                     /translation="LPERLSWMRAGVAEYIEEEIGHEEWILNDIAAAGGDVAAVRNSR
                     PDLPAELMIAYAYDLINRGNPAAFFGMVFVLEGTSVDLALNAADRIQAALGLPDTAFS
                     YLRSHGTLDQEHTQHLANILDLMTPEDQADVLHAAKVFFKLYGDIFHALPVEVAACN"
     gene            472..1257
                     /locus_tag="PLN02_12195"
     CDS             472..1257
                     /locus_tag="PLN02_12195"
                     /inference="COORDINATES: protein motif:HMM:NF006565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI53131.1"
                     /translation="MQLKGARIVLTGANGGLGRVLARELANAGAALLLTGRDPAALAR
                     IDLPAGCDYQLLQADLTHGGGIDSTVQAARSFGANILINNAGVANFGLLAEQDWNDVE
                     RILATNLQAPIRLTQALLPWLTQQPEAAVVNIGSTFGSVPFAGFTAYSAAKAGLRGFS
                     QALRRELADSTVRVIHVAPRAIDTPINPPAVRALNRTLGSATDSPELVARHIVAMLAN
                     DVAEGHIGFPERFFAWLNGFAPGLVDQGLRGKLAKIKQHATKA"
     gene            1283..>1923
                     /locus_tag="PLN02_12200"
     CDS             1283..>1923
                     /locus_tag="PLN02_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOI53132.1"
                     /translation="MSRLKSLLFAVGLGFATLIHAAPGDELIRPIQDRWAEIKYRQPE
                     DRQADLYRDLAAQAKQLSRNNPNLAAALIWEAIVVSSEAGVRGGLGGLSLAKEARALL
                     EESLKLDEKSLNGSAYTSLATLYAKVPRWPLGFGDKEKAEALFRKSLAVNPDGIDPNF
                     FYGEYLIAQDRVAEGRRHLEKALAAPPRPGRELADQGRRQEIRALLAKTAGEAR"
BASE COUNT          345 a          708 c          580 g          290 t
ORIGIN      
        1 cctgcccgag cgcctctcgt ggatgcgtgc cggtgtcgcc gagtacatcg aggaggaaat
       61 cggccacgag gaatggattc tcaacgacat cgccgccgcc ggcggcgacg tcgcagcggt
      121 acgcaacagc cgcccggacc tgccggccga actgatgatc gcctacgcct acgacctgat
      181 caaccgcggc aacccggccg ctttcttcgg catggtcttc gtcctcgaag gcaccagcgt
      241 cgacctcgcg ctgaatgccg ccgaccgcat ccaggcggcg ctgggcctgc ccgacacagc
      301 cttctcctac ctgcgctcgc acggcacgct cgaccaggaa cacacgcagc acctggccaa
      361 catcctcgac ctgatgacgc cggaagatca ggccgacgtg ctgcacgccg ccaaggtctt
      421 cttcaagctc tacggcgaca tcttccacgc cctgccggtc gaggtcgcag catgcaactg
      481 aaaggcgccc gcatcgtcct caccggcgcc aacggcggcc tcggccgggt gctcgcgcgc
      541 gaactggcca acgccggtgc cgccctgctg ctgaccggcc gcgacccggc agcactggcc
      601 cgcatcgacc tgccggccgg ctgcgactac cagttgctgc aagccgacct gacgcacggc
      661 ggcggcatcg acagcacggt ccaggccgcc cgcagcttcg gcgccaacat cctgatcaac
      721 aacgccggcg ttgccaattt cggcctgctc gccgagcagg actggaacga cgtcgaacgc
      781 atcctggcga ccaatctgca agcgccgatc cgcctcaccc aggcgctgtt gccctggctg
      841 acgcaacagc cggaggcggc ggtggtcaac atcggctcga ccttcggcag cgtgcccttc
      901 gccggcttta ccgcctattc ggcggcgaag gccgggctac gcggcttttc ccaggcgctg
      961 cggcgcgaac tcgctgacag cacggtacgc gtcatccatg tcgcaccgcg cgccatcgac
     1021 acgccgatca acccgccggc ggtgcgcgcg ctgaaccgca ccctgggcag cgccaccgat
     1081 agcccggaac tggttgcccg gcacattgtc gccatgctcg ccaacgatgt cgccgaaggc
     1141 catatcggtt tcccggaacg ctttttcgcc tggctcaacg gcttcgcccc cggtctcgtc
     1201 gaccagggcc tgcgcggcaa gctggccaag atcaaacaac acgccaccaa ggcgtgacct
     1261 tcaacagaac acaggaggca tcatgtcccg tttgaaatcc ctgcttttcg ccgtcggcct
     1321 cggcttcgcg acgctgattc acgccgcccc cggcgacgaa ctgatccgcc cgatccagga
     1381 ccgctgggcg gaaatcaagt atcgccagcc ggaagaccgg caagccgacc tctaccgcga
     1441 cctggccgcc caggcaaaac agctttcccg caacaacccc aacctcgctg ccgcgctgat
     1501 ctgggaagcc atcgtcgtct ccagcgaggc cggcgtccgc ggtggcctcg gcggcctgtc
     1561 gctggccaag gaagcccggg cgctgctcga ggaaagcctg aagctcgacg agaagtcgct
     1621 caacggctcc gcttacacca gcctggcgac gctctacgcc aaggtgccgc gctggccgct
     1681 cggcttcggt gacaaggaaa aggccgaggc gctgttcagg aagtcgctgg ccgtcaaccc
     1741 ggacgggatt gacccgaatt tcttttacgg cgagtatctg attgctcagg acagggtggc
     1801 cgaaggtcgc cgccacctgg agaaagctct tgccgcgccc cctcgccccg ggcgggaatt
     1861 ggccgatcaa ggacggcgcc aggagatccg ggcgttgctt gcaaagactg ccggagaagc
     1921 acg
//
LOCUS       DAOSDX010000612         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20375_length_1923_cov_2.651499, whole genome shotgun sequence.
ACCESSION   DAOSDX010000612 DAOSDX010000000
VERSION     DAOSDX010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20375_length_1923_cov_2.651499"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1283)
                     /locus_tag="PLN02_12205"
     CDS             complement(162..1283)
                     /locus_tag="PLN02_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOI53133.1"
                     /translation="MTDYRPGPLAWLAIIAASCGLLLLLQKALWLIVPLLLALMMYYL
                     LTPLKSLLVLRGFSHALAALMVGLAALLAMLLAGFALVAWLSAGDSNWTDNALRYIEG
                     GLRLIIDTLSLLEQRSALFARIGGAQAMTHHIDLFVEGFAASHLPVVLMGVAAWAPSV
                     LLAPFITYFMLRDGWHFKRFLVSAIPNAFFERGLDLIAQLDQTARQYFVGLIELTVVD
                     TIVLGSGLWLIGFDHALMLALVAAILAWIPYVGSIAGCALVLMVAATDFAGQPTIAYG
                     AVLLFVAVRLLDDFVFMPQTIGRSLHMHPLLSVLMLFAGGALAGITGLMLVLPLTGIC
                     YVLGRAAGQILNDDRLLARHRLATRLRRQSAAGGLDETG"
     gene            complement(1280..1759)
                     /locus_tag="PLN02_12210"
     CDS             complement(1280..1759)
                     /locus_tag="PLN02_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOI53134.1"
                     /translation="MTGFIRRKTPASDLYPLARSTLFAGLNKSELSIVESMLHTRQYQ
                     AGEIVFDEGDDGQALYIVRLGNIAIRTITTRETPVAVLGPGNFFGEMALLDQSLRSAQ
                     ARAQTESTLSALYRGDFERLLEAHSRIASRLTLQLARQLGARLRGMLEDGGKRTSTP"
BASE COUNT          379 a          550 c          671 g          323 t
ORIGIN      
        1 gtgtcgggca ggtagagcac gtagcggcgt gtcttgccgg cgaattccag cgtcacctgc
       61 tggctgcggg cttcgttccg cgacggataa tccgcgcctg ccaccaaggg tgatccggcg
      121 ctggccagcg caagcaggag gaggtaagtg gcgcgcatcg ttcagcccgt ttcgtccagt
      181 ccgcccgccg cgctctggcg acgcaggcgg gtggccaggc ggtggcgggc gagcaggcgg
      241 tcgtcgttga ggatttgtcc ggcggctcgt cccagcacgt aacagatgcc ggtcaacggc
      301 aggaccagca tcaggcccgt gatgccggcc agggctccgc cggcgaacag catcagtacg
      361 ctgagcagag gatgcatgtg caggctgcgg ccgatcgtct gcggcatgaa gacgaagtcg
      421 tcgagcaggc ggacggcgac gaagagaagc acggcgccgt aggcgatggt gggctggccg
      481 gcgaaatcgg tcgcggcgac catcagcacc agggcgcaac cggcgatcga gccgacgtag
      541 ggaatccagg ccagtatggc cgcgaccagc gccagcatca gcgcgtggtc gaaaccgatc
      601 agccagaggc cgctcccgag gacgatggtg tctacgacgg tgagttcgat caggccgacg
      661 aaatactggc gggcggtctg gtcgagttgg gcgatcaggt cgagaccgcg ttcaaagaag
      721 gcgttgggga tggcgctgac gaggaaacgc ttgaaatgcc agccgtcgcg caacatgaag
      781 taggtgatga acggcgccag cagcaccgac ggcgcccagg cggcgacgcc catcaggacg
      841 acgggcaggt ggctggcggc aaagccctcg acgaagaggt cgatatgatg agtcatcgcc
      901 tgggcgccac cgatccgggc aaaaagggcg gagcgctgct ccaggaggga caaggtatcg
      961 atgatcaggc gcaggccgcc ttcgatatag cgcagtgcat tgtcggtcca gttgctgtcg
     1021 ccggccgaca gccaggcgac cagcgcgaaa ccggcgagca gcatggcgag cagcgccgcc
     1081 aggccgacca tcagggctgc cagcgcatgg ctgaagccgc gcaggacaag cagcgatttg
     1141 agcggtgtca gcaggtaata catcatcagc gccagcagta gcggcacgat cagccacaag
     1201 gctttctgca gtaacaggag cagcccgcaa ctggcggcga tgatcgccag ccaggccagc
     1261 gggccggggc gatagtcggt catggtgtgc tggtgcgctt gccgccgtct tccagcatgc
     1321 cgcgcaggcg ggcacccaac tggcgggcga gttgcagggt cagccggctg gcgatccgcg
     1381 aatgggcttc gagcagacgc tcgaaatcgc cgcgatagag tgcgctcaag gtgctttcgg
     1441 tctgcgccct ggcctgcgcc gagcgcagcg actgatcgag cagcgccatt tcgccgaaaa
     1501 aattgccggg accgagcacg gcaaccggcg tttccctggt ggtgatcgtg cggatcgcga
     1561 tattgccgag ccgtacgata tacagcgcct gtccatcgtc gccttcgtcg aagacgattt
     1621 cgccggcctg gtattgccgg gtatgcaaca tgctctcgac gatgctgagt tcacttttgt
     1681 tgagcccggc gaagagggta ctgcgggcca gtggataaag gtccgaagcg ggcgtcttgc
     1741 ggcgaatgaa tccggtcatc gcgtttcgca gaatagtttg acgcagcgga ttatcacatt
     1801 cttcgtccgg cttgggtgag gcgtcggtat taaggaaatt cccggaaaaa gcaccttggc
     1861 aatgcgggtg agtgtggaaa tttgttaact tctcttaact tctctctgtt agtatttttg
     1921 ccc
//
LOCUS       DAOSDX010000613         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20389_length_1922_cov_2.800750, whole genome shotgun sequence.
ACCESSION   DAOSDX010000613 DAOSDX010000000
VERSION     DAOSDX010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20389_length_1922_cov_2.800750"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLN02_12215"
     CDS             complement(<1..486)
                     /locus_tag="PLN02_12215"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604896.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HOI53135.1"
                     /translation="MQTTQSMTDPALLRDAVVESFKRLSPRYQVKNPVMFVVWLGSVL
                     TTGLFVQALGGDGEAPAGFILAITLWLWFTVLFANFAEAVAEGRGKAQAAALRGLRKT
                     VWAKKLQEPHYGAKTRILPSEDLRKGDVVIVTAGEVIPGDGEAIEGVASVDESAITGE
                     SA"
     gene            complement(497..>1922)
                     /gene="kdpA"
                     /locus_tag="PLN02_12220"
     CDS             complement(497..>1922)
                     /gene="kdpA"
                     /locus_tag="PLN02_12220"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOI53136.1"
                     /translation="SYLTQMLGLGVQNFLSAATGMAVLIALARGFVQREAAALGNFWV
                     DLVRSTLYILLPLSIVLSVVLVSQGVVQTFNQHASVQLVEKLEFDQPKLGDDGQLLKD
                     AQGRPVTETMTSAEQRIAVGPAASQIAIKQLGSNGGGFFNINSSHPFENPTPLSNFLE
                     LLAILLIPAALCITFGCYVGDRRQGVAILAAMTVIFVAMLALCVGSEQSGNPLFERLG
                     LDTKASELQAGGNMEGKETRFGVVNSALWATATTAASNGSVNAMHDSFTPLGGLVPMW
                     LMQLGEVVFGGVGSGLYGMIIFALVAVFVAGLMIGRTPEYLGKKIEAFEVKMAAVAIL
                     VPPLVVLLGTAIAVMADAGRAGTANPGAHGFSEILYALSSAGNNNGSAFGGLSANTPF
                     YNTLLGFAMLFARYWLIVPVLAIAGSLAAKKSIPPGAGTLPTHTPLFVMLLIGTVIVV
                     GALTFLPALALGPIVEHLQMIGAQ"
BASE COUNT          382 a          597 c          634 g          309 t
ORIGIN      
        1 tgccgattcg ccggtgatcg ccgattcgtc gaccgaggcg acgccttcga tggcttcgcc
       61 gtcaccggga atgacttcgc cagcggtgac gatgacgaca tcgcccttgc gcagatcctc
      121 cgaaggcagg atgcgcgtct tcgcgccgta atgcggttcc tgcagtttct tggcccacac
      181 cgtcttgcgc agcccgcgca gggcggcggc ctgcgccttg ccgcggcctt cggcgaccgc
      241 ttcggcgaag ttggcgaaga gcacggtgaa ccacagccac agcgtgatgg ccaggatgaa
      301 tccggccggg gcctcgccat cgccacccag cgcctggaca aacagcccgg tggtgagcac
      361 gctgcccaac cagacgacga acatcaccgg gttcttgacc tggtaacgcg gcgacaggcg
      421 tttgaaggac tcgaccacgg cgtcgcgcag cagcgccgga tcggtcatgg attgtgtcgt
      481 ttgcatcaaa ttctccttat tgggcgccaa tcatttgcag gtgctcgaca atcggcccca
      541 atgccagtgc aggcaggaag gtcagcgcac cgaccacgat caccgtgccg atcagcaaca
      601 tgacgaacag cggcgtatgg gtcggcaagg tgccggcccc cggcggaatg ctcttcttgg
      661 cggccaacga accggcaata gccagtaccg gaacgatcag ccagtagcgg gcgaacagca
      721 tggcgaagcc gagcagcgtg ttgtagaaag gcgtattcgc cgacaggccg ccgaaggcgc
      781 tgccattgtt gttgccggcc gacgacaggg cgtagaggat ttccgagaag ccgtgcgcgc
      841 caggattggc ggtgccggcc cggccggcgt ccgccatcac ggcgatggcg gtgccgagca
      901 ggacgaccaa cggcggcaca aggatggcga cagcagccat cttgacctcg aaggcctcga
      961 tcttcttgcc caggtactcc ggcgtgcggc cgatcatcag gccggcgacg aagactgcca
     1021 cgagcgcgaa gatgatcatg ccgtacaacc cggaaccgac gccgccgaaa accacctcgc
     1081 cgagttgcat cagccacatc ggtaccagac caccgagcgg cgtgaaggag tcgtgcatgg
     1141 cattgaccga accgttcgag gcggcggtgg ttgctgtcgc ccacagcgcc gaattgacca
     1201 caccaaagcg ggtttccttg ccttccatgt tgccgccggc ctgcaattcg gaggccttgg
     1261 tatcgagacc gaggcgctcg aacagtgggt tgcccgactg ctcggagccg acgcagaggg
     1321 ccagcatggc cacgaaaatg acggtcattg ccgcaagaat ggcgacgccc tggcgccggt
     1381 cgccgacgta acagccgaag gtgatgcaca aggcggccgg gatcaggaga atggccagga
     1441 gttcgaggaa gttggacagc ggagtcggat tctcgaaggg gtgggacgaa ttgatgttga
     1501 agaaaccgcc accgttgcta cccagttgct tgatggcgat ctgcgacgca gcaggaccga
     1561 ccgcgatgcg ctgctcggcg ctcgtcatcg tttcggtgac cggccggcct tgcgcatcct
     1621 tgagcagctg gccgtcgtcg cccagcttgg gctggtcgaa ttcgagcttt tcgacgagct
     1681 ggacgctggc gtgctggttg aacgtctgca cgacgccctg gctgaccagg acgaccgaca
     1741 gtacgatgga cagcggcaac aggatgtaaa gcgttgaacg gaccaggtcg acccagaaat
     1801 tgcccagagc cgcggcttcg cgctgcacga agccgcgcgc cagggcgatg aggaccgcca
     1861 tgccggtggc ggcggacagg aagttctgca cgccgagacc gagcatctgc gtcaggtaac
     1921 tc
//
LOCUS       DAOSDX010000614         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20410_length_1921_cov_2.349411, whole genome shotgun sequence.
ACCESSION   DAOSDX010000614 DAOSDX010000000
VERSION     DAOSDX010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20410_length_1921_cov_2.349411"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..981
                     /gene="nadC"
                     /locus_tag="PLN02_12225"
     CDS             118..981
                     /gene="nadC"
                     /locus_tag="PLN02_12225"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOI53137.1"
                     /translation="MSIEFDYPLAAEITRNVTAAIVEDVGAGDLTAALVPGERRVRAR
                     VIAREPGVLCGAAWFDECVRRLDPTATVSWQAADGERIGADQVLCTIDGNGRALLTAE
                     RSALNFLQLLSAVASKARIYADAIAGTRAQVVDTRKTLPGLRVAQKYAVRAGGGGNHR
                     LALWDAILIKENHIHAAGGIAQAMAAAQKVAAAAADRCKFIQVEVENLDELRQALDAG
                     ATMILLDNFSCALLREAVALNAARAVLEASGNVSLESIREIAETGVDRISVGALTKDV
                     KALDLSMRFVD"
     gene            complement(991..1614)
                     /locus_tag="PLN02_12230"
     CDS             complement(991..1614)
                     /locus_tag="PLN02_12230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI53138.1"
                     /translation="MSFDHEAFWSARYRDAGEDYLFGTAPNAFLSAQAGRFAAGMSVL
                     AVADGEGRNSVWLAEQGCAVTATEISPVALAKAAKLARARRVAVDFIEADLLRWDWPL
                     AAFDAVVGIFIQFVGPAEREILFAGMQAAVKPGGLLLLHGYTPKQLEYRTGGPSAVEN
                     LYTAELLRSAFAGWELLELREHEAVIGEGRGHAGRAALIDLVARKPA"
     gene            complement(1611..>1921)
                     /locus_tag="PLN02_12235"
     CDS             complement(1611..>1921)
                     /locus_tag="PLN02_12235"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288117.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOI53139.1"
                     /translation="SGRSTHIDRINTIRYAQGRYNVMLDTHTADGLKVALEQRQPGQT
                     MIVLETAQPAKFEETIREALGTEPVRPAELAGIENLEQHVVVMDPDVAAVKQFVVERV
                     "
BASE COUNT          315 a          684 c          628 g          294 t
ORIGIN      
        1 ctcctgtggc aggactagcc gctgttggat catccgcgcc gggcaagggt tcccggcttc
       61 tggccaccgc cgccgggcgt tacaatgcgc cacccgcaac tttcccgccc acccgccatg
      121 tccatcgaat tcgactatcc gctcgccgct gaaatcaccc gcaacgttac cgccgccatc
      181 gtcgaggatg tcggcgccgg cgacctgacg gcggccctcg ttcccggcga acgccgcgtc
      241 cgcgcccgcg tcatcgcgcg cgaacccggc gtgctgtgcg gcgccgcctg gttcgacgaa
      301 tgcgtgcgcc gtctcgaccc gacggccacg gtgagctggc aggcggccga cggcgagcgc
      361 atcggcgccg accaagtgct gtgcaccatc gatggcaacg gccgcgccct gctcaccgcc
      421 gagcgcagcg cgctgaactt cctgcaactg ctgtcggcgg tggcgagcaa ggcgcgcatc
      481 tacgccgatg cgatcgccgg cacccgggcg caggtggtgg atacgcgcaa gaccctgccc
      541 ggcctacgcg tcgcccagaa atacgcggtg cgtgccggcg gcggcggcaa tcaccggctg
      601 gcgctgtggg acgccatcct gatcaaggaa aaccacatcc acgctgccgg cggcattgcc
      661 caggcgatgg ccgccgcgca gaaagtcgcg gcagcagcgg ccgaccgctg caagttcatt
      721 caggtggaag tggaaaacct cgacgaactg cgtcaggcgc tggacgccgg cgcgacgatg
      781 atcctgctcg acaatttctc ctgcgccctg ctccgcgagg cggtggcgct caacgccgcc
      841 cgcgccgtcc tcgaagcctc aggcaacgtc agcctggaga gcatccggga aatcgccgaa
      901 accggcgtcg accgcatctc ggtcggcgcg ctgaccaagg acgtcaaggc gctcgacctg
      961 tcgatgcgct tcgtcgactg agcgccggcc tcaggccggc ttgcgggcga ccaggtcgat
     1021 cagcgcggca cgcccggcgt ggccgcgccc ttcgccgatc accgcctcgt gctcgcgcag
     1081 ttcgagcagt tcccagccgg cgaaggcgct gcgcagaagt tcggcggtgt acaggttttc
     1141 cacggcggac gggccgccgg tccggtactc aagctgtttt ggcgtataac catgcaagag
     1201 caacaggcca ccgggcttga ccgccgcctg catgccggca aacaggattt cccgttcggc
     1261 cggcccgacg aactggatga agataccgac cactgcgtcg aaggcagcaa gcggccagtc
     1321 ccagcgtagc aaatcggcct cgatgaaatc gaccgccacc cggcgggcgc gcgccagctt
     1381 ggcggctttg gccaaggcca ccggcgaaat ttcggtggcg gtgacggcac agccctgctc
     1441 ggccagccac accgaattgc gcccttcgcc gtcggccacc gccagcaccg acatgccggc
     1501 ggcaaagcgc ccggcctggg cgctcaggaa agcgttcggc gcggtgccga agaggtaatc
     1561 ctcgccggca tcgcgatagc gcgctgacca gaaggcctcg tgatcaaagc tcatacgcgc
     1621 tcgacaacga actgcttgac cgccgcgacg tccgggtcca tgaccacgac gtgctgctcc
     1681 aggttctcga tgccggccag ttcggccggg cgcaccggct cggtgcccag cgcttcgcgg
     1741 atggtttcct cgaacttggc cggctgcgcg gtttccagca cgatcatggt ctgccccggc
     1801 tgacgctgtt cgagcgccac cttgaggccg tcggcggtgt gcgtgtcgag catcacgttg
     1861 taacgcccct gggcgtagcg aatcgtgttg atgcggtcga tatgcgtgct gcgcccggaa
     1921 a
//
LOCUS       DAOSDX010000615         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20423_length_1920_cov_2.947453, whole genome shotgun sequence.
ACCESSION   DAOSDX010000615 DAOSDX010000000
VERSION     DAOSDX010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20423_length_1920_cov_2.947453"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="kdsA"
                     /locus_tag="PLN02_12240"
     CDS             <1..715
                     /gene="kdsA"
                     /locus_tag="PLN02_12240"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOI53140.1"
                     /translation="LGIPFIYKSSYDKANRSSGTSVRGPGIDGGLKILDNVRAQVGVP
                     VLTDVHSEADVAQVASVVDVLQTPAFLCRQTDFIHAVAACGKPVNIKKGQFLAPGDMK
                     NVVAKAREVNGGADNILVCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLP
                     GGQGTVSGGQREFVPVLARAAVAVGIAGLFMETHPCPEKAFSDGPNSWPLDRMASLLT
                     TLVALDRAVKAAGFEEFKI"
     gene            765..>1920
                     /gene="eno"
                     /locus_tag="PLN02_12245"
     CDS             765..>1920
                     /gene="eno"
                     /locus_tag="PLN02_12245"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOI53141.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKGRYLGKGVMQAVENINTEISEAIIGLDAQEQAFVDQTMIELDGTDN
                     KSRLGANAILAVSMAVAKAAAEESGLPLYRYFGGMAPMQMPVPMMNIINGGAHANNSL
                     DIQEFMVMPVGANTFREALRCGAEIFHALKKLLDKKGHSTAVGDEGGFAPNLGSHAEA
                     LEVIMQAIEAAGYVPGQDVLLALDCAASEFYKDGRYHLSGEGLQLTAEQFTDYLANLA
                     DQFPIVSIEDGMAEGDWDGWKLLTDRLGSKVQIVGDDLFVTNTRILKEGIQKGIGNSI
                     LIKINQIGTLSETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGLNAG"
BASE COUNT          370 a          642 c          587 g          321 t
ORIGIN      
        1 gctcggcatt cccttcatct acaagtcgtc ctacgacaag gccaaccgca gctccggcac
       61 ctcggtgcgc ggaccaggca tcgacggcgg gctgaaaatc ctggacaacg ttcgtgccca
      121 ggtcggcgtg ccggtattga ccgacgtgca cagcgaagcc gacgtcgccc aggtggcgag
      181 cgtcgtcgac gtcctgcaga cgccggcctt cctctgccgg cagaccgatt tcatccacgc
      241 cgtggccgcc tgcggcaagc cggtgaacat caagaagggc cagttcctgg cgccgggtga
      301 catgaagaac gtcgtcgcca aggcgcgcga ggtcaacggc ggcgccgaca acatcctggt
      361 ctgcgaacgc ggcgccagct tcggctacaa caacctggtg tccgacatgc gctcgctggc
      421 aatcatgcgc gagaccggct gcccggtcgt cttcgatgcc acccattcgg tgcagctgcc
      481 gggtgggcag ggcacggtct ccggcggtca gcgcgagttc gttccggtgc tggcgcgggc
      541 ggcggttgcc gtgggcatcg ccggcctgtt catggaaacc catccctgcc cggaaaaggc
      601 cttttccgac ggcccgaatt cctggccgct cgaccgcatg gcaagcctgc tgactaccct
      661 ggtcgccctt gatcgggcgg tcaaggctgc cggtttcgaa gagtttaaga tttaaagttt
      721 ttgtttaaga agtatctgta accttttgtt tataaggaag agacatgagt tctatcgttg
      781 atgtcgtcgc ccgcgagatt ctggattccc gcggcaaccc cacggtcgag gccgatgtgc
      841 tgctggaaag cggcgtcatg ggccgcgccg cggtgccgtc cggcgcctcc accggctcgc
      901 gcgaagccat cgaactgcgt gacggcgaca agggccgtta cctcggcaag ggcgtcatgc
      961 aggcggtcga gaacatcaac accgaaatct cggaagcgat catcggtctc gacgcccagg
     1021 aacaggcctt cgtcgaccag acgatgatcg aactcgacgg caccgacaac aagtcgcgcc
     1081 tcggcgccaa cgccatcctc gccgtctcga tggcggtcgc caaggccgct gctgaagaat
     1141 ccggcctgcc gctctaccgc tacttcggcg gcatggcgcc gatgcagatg ccggtgccga
     1201 tgatgaacat catcaacggc ggcgcgcacg ccaacaacag cctggacatc caggaattca
     1261 tggtgatgcc ggtcggcgcc aacaccttcc gcgaagcgct gcgctgcggc gccgaaatct
     1321 tccacgcact gaagaagttg ctggacaaga agggccattc gaccgccgtc ggcgacgaag
     1381 gcggctttgc ccccaacctg ggtagccacg ccgaagccct ggaagtgatc atgcaggcga
     1441 tcgaggccgc cggttacgtg cccggccagg acgtgctgct ggcgctcgac tgcgccgcct
     1501 ccgagttcta caaggacggc cgctaccatc tgtccggcga aggcctgcaa ctgaccgccg
     1561 agcaattcac cgactacctc gccaacctgg ccgaccagtt cccgatcgtc tcgatcgagg
     1621 acggcatggc cgaaggcgac tgggacggct ggaagctgct gaccgaccgt ctgggcagca
     1681 aggtgcagat cgtcggcgac gacctcttcg tcaccaacac ccgcatcctc aaggaaggca
     1741 tccagaaggg catcggcaac tcgatcctga tcaagatcaa ccagatcggc accctgtcgg
     1801 aaaccttcgc cgccatcgag atggccaagc gcgccggcta caccgccgtg atctcgcacc
     1861 gttccggcga aaccgaggac agcaccatcg ccgacatcgc cgtcggtctc aacgccggtc
//
LOCUS       DAOSDX010000616         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20465_length_1917_cov_3.047261, whole genome shotgun sequence.
ACCESSION   DAOSDX010000616 DAOSDX010000000
VERSION     DAOSDX010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20465_length_1917_cov_3.047261"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLN02_12250"
     CDS             complement(<1..253)
                     /locus_tag="PLN02_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53142.1"
                     /translation="MDKRQFLLDQIADKVKVFKAESEKHKRLHRRLRYAVFLLTSLSA
                     LLAGLALKFPESAAAISVVILLVSAGSGVLSSVEGLRKPA"
     gene            460..>1917
                     /locus_tag="PLN02_12255"
     CDS             460..>1917
                     /locus_tag="PLN02_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI53143.1"
                     /translation="MNRESAQPSLGAIFQQAIALHQAGRLPDALEAYQHVIGHIRQYV
                     EAFLNAGSLLERFGQLAEALQNYDAAIALQPAIPDSHCLRGNLLFRLHRFDEALAAYE
                     RAISLCPGFPEALLNRGLLLAHLGRVDEALSSYTRAIESKPDYLGAHLNRGQFLKELR
                     RFDEALASYEQALRIDPNAADVYNNRGILLGELRQHEAALASYRQAIALCPDHGDAHL
                     NLGVLFAELDQVDEALASYDRALAIRPDYPMALNNKAILLKECGRFAEAQACCERALR
                     LQPDFPEAQYTRAELLLLTGDYARGWPLFEARWQSRYRPGTSPLAATPLWTGEPLAGK
                     TLLIEPEVGFGDQIMFARYARLAEQQGARVLLLASAPLCELFTTLGGAITVLEDGHGA
                     PLVDFRCPIMSLPHALRTMPDRVPAAFPYLGVPAARRALWRGRLGAARAPRIGLVWSG
                     AADRYQDRNPRTARSLSLAALAPLLCLPFAFHALHK"
BASE COUNT          293 a          653 c          614 g          357 t
ORIGIN      
        1 cggcaggctt gcgcaggcct tcgaccgagc tcaacacgcc gctgccggcg gaaaccagca
       61 ggatgacgac gctgatcgcc gcggcggact ccggaaactt cagtgccagc ccggcgagca
      121 gagccgatag cgaagtcagc aggaagaccg cgtaacgcag ccggcgatgc aggcgcttgt
      181 gcttttccga ctccgccttg aagaccttca ccttgtcggc gatttgatcg agcaggaact
      241 gacgtttgtc catggcgttt ccccctctcc gctggcggcg aacatgacat tgggcagcgc
      301 cggggcggct tggttcaaag tgctgccaat ggcttggctg ctggcttgcg gcggaaatct
      361 tcatggcttg ctgcgatcgc tattggccag tctcttgtca tgcggcatga tgcgatcatt
      421 tgtcggcgct cgccgctatc cctctggatg ctcatggtca tgaatcgcga atccgctcaa
      481 cccagtctcg gtgccatctt tcaacaggcg attgcgctgc accaggccgg gcgcctgcct
      541 gatgcgctgg aggcttacca gcacgtcatc ggccacattc ggcaatacgt cgaggctttc
      601 ctcaacgccg gcagcctgct ggaacgattc ggccagttgg ccgaggcgct gcagaattac
      661 gacgcggcga tcgcgctgca gccggcgatt cccgacagtc actgcctgcg cggcaacctg
      721 ctgtttcgcc tgcatcgctt cgacgaggcg ctggccgctt acgagcgggc gatttccctg
      781 tgccccggtt ttcccgaggc gctgctcaac cgtggcctgc tgctggcgca tctgggccgt
      841 gtcgacgagg cgctgagcag ctacacgcgg gcgatcgaga gcaagcccga ttatctcggc
      901 gcccacctca atcgcggcca gtttctcaag gagttgcggc gtttcgacga ggcgctggcg
      961 agctacgagc aggccctgcg tatcgacccg aacgccgctg acgtctataa caaccgtggc
     1021 atcctgctcg gcgaactgcg ccagcacgag gcggcgctcg ccagctaccg gcaggccatt
     1081 gcgctgtgtc ccgatcatgg cgacgcccat ctcaatctcg gcgtcctgtt cgccgaactc
     1141 gaccaggtcg acgaggcgct ggccagttac gaccgggcgc tggcgattcg ccccgattac
     1201 ccgatggcct tgaacaacaa ggcgatcctg ctcaaggaat gcgggcgctt tgccgaggcg
     1261 caggcctgct gcgagcgggc gctccgcctc cagccggatt ttcccgaagc ccagtacacg
     1321 cgcgccgaat tgctgctgct caccggggat tatgcccgcg gctggccgct cttcgaggcg
     1381 cgctggcaga gccgctaccg tcccgggaca tcgcctctgg ccgcaacgcc gctttggacc
     1441 ggtgagccgc tggcgggaaa aaccctgctg atcgaaccgg aagtcggctt cggcgaccag
     1501 atcatgttcg cgcgctatgc ccgtctggcc gaacagcagg gcgcccgggt cctgctcctg
     1561 gcatcggcgc cgctatgcga gctgtttacc accctgggcg gggcgatcac cgtgctggaa
     1621 gatggccatg gggcaccgct ggtcgatttt cgctgcccga tcatgagcct gccgcacgcg
     1681 ctgcgcacga tgccggaccg cgtgccggcg gccttccctt atttgggcgt gccagcagcg
     1741 cgccgggcac tctggcgcgg gcgcctcggc gcggcgcggg cgccgcggat cgggctggtc
     1801 tggtcgggtg ctgcggaccg ctaccaggac cgcaatccgc gcaccgcccg cagcctgtcg
     1861 ttggccgccc tcgccccgct gctgtgcctg ccttttgctt tccatgccct gcacaag
//
LOCUS       DAOSDX010000617         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20526_length_1912_cov_2.639742, whole genome shotgun sequence.
ACCESSION   DAOSDX010000617 DAOSDX010000000
VERSION     DAOSDX010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20526_length_1912_cov_2.639742"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1912
                     /locus_tag="PLN02_12260"
     CDS             <1..>1912
                     /locus_tag="PLN02_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI53144.1"
                     /translation="NVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVITE
                     DDCGTKNGFAVKALVEGGEVIEPLRERILGRVTVDDLIDPETQETVIFAGTMLDEDLV
                     DLIDKLGIDEVKVRTPLTCDTRYGLCAKCYGRDLGRGTMVNAGESVGVIAAQSIGEPG
                     TQLTMRTFHVGGAASRAAVASQVEAKSAGTVRFTSNMRYVTSAKDEKVVISRSGEVLI
                     TDDHGRERERHKVPYGAMLTVDEGKAVKAGTKLATWDPHTRPIITEYAGTVRFENVEE
                     GVTVAKQIDDVTGLSTLVVIDNKRGGKASAKGLRPVVKLLDDGGQEVKVHGSEHAVSI
                     AFQVGSIISVVDGQQVGVGDVLARMPQESAKTRDITGGLPRVAELFEARTPKDASVLA
                     EVTGTISFGKDTKGKQRLVITDLEGEQHEFLISKDKHVLVHDGQVVNKGEKIVEGVRK
                     MGPLKYRMFMAALAAGRKAAESINHGLQPEGLAARIAAAADHKVFAQVRERFGGRIRY
                     MFSAGAALDGDTLMFFRGLGLMLIEGYGLSESSPVTHANPLTGVRKIGSIGLALPDTD
                     CRIVDLDFGTDDVPTGETGELVVKGPQVMKSYWKMESETADALRDGWLYTGDIATMDE
                     DGYVFVIDRKKDL"
BASE COUNT          386 a          589 c          603 g          334 t
ORIGIN      
        1 tgaacgttct ccagtacttc atctcgaccc acggtgcccg taagggtctg gccgacaccg
       61 cgctgaagac tgccaactcc ggttacctga cgcgccgtct ggtcgacgtg acgcaggatc
      121 tggtgatcac cgaagacgat tgcggcacca agaacggctt cgccgtcaag gcgctggtcg
      181 aaggcggtga agtcatcgag ccgctgcgcg agcgtatcct cggtcgcgtc acggtcgatg
      241 acctgattga tccggaaacc caggaaaccg tcatcttcgc cggcaccatg ctcgacgaag
      301 atctggtcga cctgatcgac aagctcggca tcgacgaagt caaggttcgt accccgctca
      361 cctgcgacac ccgctacggc ctgtgcgcca agtgctacgg tcgtgacctc ggtcgcggca
      421 ccatggtcaa cgccggtgaa tcggtcggcg tcatcgccgc ccagtcgatc ggcgaaccgg
      481 gtacccagct cacgatgcgg accttccacg tcggcggtgc ggcctcgcgg gctgccgtcg
      541 cctcgcaagt tgaagccaag tcggccggta cggtccgctt cacctcaaac atgcgttacg
      601 tgaccagcgc caaggacgag aaggtcgtca tttcccgttc cggcgaagtg ctgatcaccg
      661 acgaccatgg ccgcgagcgc gaacgccaca aggtgccgta cggcgccatg ctgacggtcg
      721 atgaaggcaa ggcagtcaag gccggcacca agctggcgac ctgggatccg catacccggc
      781 cgatcatcac cgaatacgcc ggtaccgtcc gcttcgagaa cgtcgaagag ggggtcaccg
      841 tcgccaagca gatcgacgat gtgaccggtc tgtccactct ggtcgtgatc gacaacaagc
      901 ggggcggcaa ggcttcggcc aagggcttgc gcccggtcgt caagctgctc gacgatggcg
      961 gtcaggaagt caaggtgcac ggcagcgagc acgcggtgtc gatcgccttc caggtcggct
     1021 cgatcatctc ggtggtcgat ggccagcagg tcggcgttgg cgatgttctc gcccgcatgc
     1081 ctcaggaatc ggcgaagacc cgtgacatta ccggcggtct gccgcgcgtc gccgaattgt
     1141 tcgaagcacg caccccgaag gatgcttcgg tgctggcgga agtcaccggc acgatcagct
     1201 tcggcaagga caccaagggc aagcaacgtc tggtcatcac cgatctcgag ggcgaacagc
     1261 acgagttcct gatctccaag gacaagcatg tgctggtgca cgacggtcag gtggtcaaca
     1321 agggcgagaa gatcgtcgaa ggcgtgcgca agatggggcc gttgaaatac cgcatgttca
     1381 tggcggcgct tgccgcagga cgcaaggcgg cggaaagcat caatcacgga cttcagcccg
     1441 aaggccttgc cgcccgaatc gccgccgccg cggatcacaa ggtattcgcg caggtacgtg
     1501 aacgcttcgg cggacgcatt cgctatatgt tttccgccgg agcggcgctg gacggcgaca
     1561 ccctgatgtt tttccgcggg ctcggcctga tgctgattga gggatacggc ctgtcggaat
     1621 ccagccccgt gacgcatgcc aatccgttga ccggtgtccg caagatcggt tccatcgggc
     1681 ttgccctccc cgacacggac tgccgaattg tcgacctcga ctttggtacg gacgatgtgc
     1741 caacggggga gaccggcgaa ctggttgtca agggtccaca ggttatgaaa tcctactgga
     1801 agatggaaag cgaaacagcc gacgctctgc gcgacggatg gctttacacc ggagacatcg
     1861 ccacgatgga tgaggacggc tacgtctttg tcatcgatcg caagaaggac ct
//
LOCUS       DAOSDX010000618         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20551_length_1910_cov_4.043127, whole genome shotgun sequence.
ACCESSION   DAOSDX010000618 DAOSDX010000000
VERSION     DAOSDX010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20551_length_1910_cov_4.043127"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLN02_12265"
     CDS             complement(<1..952)
                     /locus_tag="PLN02_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOI53145.1"
                     /translation="MSRHADAIRLLLAQGPLSARQLVKKTGISQPTVSRALAVLGDEV
                     VRIGAGPAIQYALRDAGRGAVAAPVFRVSDEGQILELGRLLPMRPAGFVMLQADGVTL
                     HSDGLPWWLFDMRPQGYLGRAYATAHAAGLGLPANPEQWNDTDVLQALLAQGHDAIGN
                     LLIGEAARERFLAMPAPQPVDRATSYPALARAASAGELPGSSAGGEQPKFCAYSERGH
                     VLVKFSAADDNPVSERWRDLLLAEHLALAVLGMDSEVFDFAGQRFLEVPRFDRVGQLG
                     RVGVFSLRALDAEFVGDAAAPWPSLVARLAAVPGIDDLAMT"
     gene            1157..1765
                     /locus_tag="PLN02_12270"
     CDS             1157..1765
                     /locus_tag="PLN02_12270"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOI53146.1"
                     /translation="MSQEQAREPVQALIGSRIFVLRGQRVMLDSDLAELYGVETRVLV
                     QAVKRNQERFPEDFMFILENHDLTALRSQIVISNGRRGGRRTAPYAFTEQGVAMLSSV
                     LNSPRAIAINIEIMRTFVRVRELANTHGELAERLNELEEKAEALALQQDNFAHTTRAQ
                     LKQVFEAIRQLMTPPEPSKRPIGFVQPADEAGKPKAVKERRG"
BASE COUNT          384 a          608 c          596 g          322 t
ORIGIN      
        1 tggtcatcgc cagatcgtcg atgccgggca ctgcggccag gcgggcgacc agcgatggcc
       61 agggcgcggc ggcatcgccg acgaattcgg cgtcgagcgc gcgcagcgaa aagacgccga
      121 cgcgacccaa ttgcccgaca cggtcgaagc gcggcacttc caggaagcgc tggccggcga
      181 aatcgaacac ttcgctgtcc atgcccagca ccgccagtgc cagatgctcg gccagcagta
      241 ggtcgcgcca gcgttcgctg accgggttgt cgtccgccgc cgaaaatttc accagcacat
      301 ggccgcgctc gctataggcg cagaacttcg gctgctcgcc gccggccgac gagccaggca
      361 gttcgccggc gctggcggcg cgcgccagtg ccggatagct cgtcgcccgg tcgaccggct
      421 gcggggccgg catggccaga aagcgctcgc gcgccgcttc gccgatgagc aggttgccga
      481 tggcgtcatg gccctgcgcc agcagcgcct gcaggacgtc ggtgtcgttc cactgttccg
      541 gattcgccgg caggccgagg ccggccgcgt gggccgtcgc ataggcgcgg ccgagatagc
      601 cctgcggccg catgtcgaac agccaccagg gcaggccgtc gctgtgcagc gtgacgccat
      661 cggcctggag catgacgaaa cccgccggcc gcatcggcaa cagccggccc agttcaagaa
      721 tctgcccctc gtcgctgact cggaacaccg gcgcggcgac ggccccccgc ccggcatccc
      781 gcagcgcgta ctgaatagcc ggcccggcgc cgatccgaac cacctcgtca cccagcaccg
      841 ccagcgcccg cgacaccgtc ggctggctga tccccgtttt ttttacgagt tgccgcgcgc
      901 tcaaggggcc ctgagcgagc agcaggcgaa tggcgtcggc gtggcgcgac atgatttgaa
      961 tggatcaacg aatagataag cgaatagcta ttctaagcgg acaatggcca ggcgtcacga
     1021 aatcctggct ggcaaagcca tcttcttccg acaaaccgcc gtgattggga tgtcctgaaa
     1081 atccaattgg gatattctgg cgcgatgctg aatcatgggc aggccagtcc tgcaacttct
     1141 gacaggagga agtgaaatga gccaggaaca agcacgcgag cccgtgcagg cgctgatcgg
     1201 tagccggatt tttgttttgc gggggcagcg ggtaatgctc gacagcgatc ttgcagaact
     1261 ctatggcgtc gaaacccgtg tgctggttca ggcggtaaag cgcaaccagg agcgctttcc
     1321 cgaagacttc atgtttattc tggaaaatca tgaccttacc gctttgagat cacaaattgt
     1381 gatctcaaac ggcaggcggg gcggtcgccg aacggcaccg tatgccttca ccgaacaagg
     1441 cgtcgccatg ctctcgtcgg tgctgaacag cccgcgtgcc atagcgatca acatcgagat
     1501 catgcgcacc ttcgtgcgtg tccgtgaatt ggccaatacc cacggcgaac tggccgagcg
     1561 cctcaatgaa ctggaggaaa aagccgaagc cctggccctc cagcaggaca acttcgcaca
     1621 caccacccgc gctcagctca agcaggtgtt tgaggcgatt cgtcagttga tgaccccgcc
     1681 ggaacccagc aagcgtccta tcggcttcgt gcagccagct gacgaagccg gaaagccgaa
     1741 agcagtcaaa gaaaggcgtg gatgatcgct ggcgatcaat ttcgcctgga acgattcccg
     1801 ccgcgggtcg gttgttgctc gcgggccgtc agcagacaat cgatcccggt acagatatgt
     1861 tttaggggta tcaagtgcat tcgtccccga aaaatgagct tggcccgttt
//
LOCUS       DAOSDX010000619         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20561_length_1910_cov_2.736927, whole genome shotgun sequence.
ACCESSION   DAOSDX010000619 DAOSDX010000000
VERSION     DAOSDX010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20561_length_1910_cov_2.736927"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLN02_12275"
     CDS             <1..563
                     /locus_tag="PLN02_12275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI53147.1"
                     /translation="GNVPINEHIDRMLLAGIDFVAAYIDIDNFKPFNDNFGYRRGDDV
                     IQMLGRLIGEVVETRQDFVGHIGGDDFFVVFQSPDWEMRCWQLVSAFTERIDALIGPD
                     DPGRQGYLAENRRGEMVFHALPTLSVGAVRIKSGSFESHREVAAAASVAKKQAKKRAR
                     EPGTPGGGNVFVERRGAAELAANQLN"
     gene            complement(570..1202)
                     /locus_tag="PLN02_12280"
     CDS             complement(570..1202)
                     /locus_tag="PLN02_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOI53148.1"
                     /translation="MKTSQKGFTLIEIAIVLVIIGLLLGGVMKGQELITQSKIRSVEK
                     EFDGVTVAVLGYRERYKALPGDDLQAKARWTSSDDGNGDGEIGGNFNSSTATDESRLL
                     WQHLRAAGLVTGHTVPTQQPANAAGGIIGVQTDISDGAAKILEGTLVCTTNLPAAVAN
                     AIDAAQDEGLPEQGSVRGFKQSASNTPDYAEQITAYVDDGKTLYTLCKQI"
     gene            complement(1232..>1910)
                     /locus_tag="PLN02_12285"
     CDS             complement(1232..>1910)
                     /locus_tag="PLN02_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOI53149.1"
                     /translation="LTERLAQRGFSLIELSIVLIVFGLLASGMVGSLSGQRRLADERR
                     AQQQLDEALEALYGFAITNGRLPCPAEAPLASSEEAAGREACPLEHGVLPWRTLGVAE
                     LDPWGQRLSYYAASAFSANVPSGARAAFTLESKGGASVRLAHDSGAALANELPAVVVS
                     HGANGHLGYRSNGGKLAGGSADEAENADQDSVFVSHLADADYDDLVCWLPPAILATRM
                     LAAGRLP"
BASE COUNT          292 a          584 c          671 g          363 t
ORIGIN      
        1 cgggcaatgt gccgatcaac gagcacatcg atcgcatgct gctcgccggc atcgacttcg
       61 tcgccgccta catcgacatc gacaatttca agcctttcaa cgacaacttc ggctaccgcc
      121 ggggcgacga cgtgatccag atgctcggcc gcttgatcgg tgaagtggtc gaaacgcgtc
      181 aggatttcgt cggccatatc ggcggcgacg acttcttcgt ggtcttccag agcccggact
      241 gggaaatgcg ttgctggcaa ctggtcagcg ctttcaccga gcgcatcgat gccctgattg
      301 gcccggatga tccggggcga cagggttacc tggcggagaa tcggcgtggc gaaatggtct
      361 tccacgcgct gccgacgctg tcggtcggtg cggtcaggat caagtccggc agcttcgaat
      421 cgcatcgcga ggttgccgcg gccgcctcgg tggcgaagaa acaggccaag aagcgtgccc
      481 gcgaaccggg cacgcccggt ggcgggaatg tcttcgtcga gcggcgcggt gctgccgaac
      541 tggcggcgaa ccagctcaat tgacggggct cagatctgct tgcacagggt gtagagcgtt
      601 ttgccgtcgt cgacgtaagc cgtgatctgc tcggcatagt ccggggtatt gctggcgctc
      661 tgcttgaatc cgcgcacgct gccctgctcc ggcagacctt cgtcctgtgc cgcgtcgatg
      721 gcgttggcca ccgccgccgg caggttggtg gtgcacacca aggtgccttc caggattttc
      781 gctgcgccat cgctgatgtc cgtctgcacg ccgatgatgc cgccggcggc gttggccggt
      841 tgctgcgtcg gcacggtgtg gccggtgacc aagccggcgg cgcgcaggtg ttgccagagc
      901 aggcggctct cgtcggtggc ggtgctggaa ttgaagttgc cgccgatctc gccgtcgcca
      961 ttgccgtcgt ctgaagatgt ccagcgtgcc ttagcctgca agtcgtcgcc gggcagcgcc
     1021 ttgtagcgct cacggtagcc gaggacggcg acggtgacgc cgtcgaattc cttctcgacg
     1081 ctgcggatct tgctctgcgt gatcagttcc tgtcccttca tgacgccgcc gagcaggagg
     1141 ccgatgatga cgaggacgat ggcgatttcg atcagggtga agcctttctg cgaggttttc
     1201 atgcttgact ccgtggtggt gggttgagaa atcagggcag acggccggcg gcgagcatcc
     1261 gcgtggcgag gatggcgggc ggcagccagc acaccaggtc gtcgtagtcg gcgtccgcca
     1321 ggtggctgac gaagacgctg tcttggtcgg cgttttccgc ttcatcggcc gagccgccgg
     1381 ccagcttgcc gccgttgctg cgatagccga gatggccgtt ggcgccgtgg ctgacgacga
     1441 cggccggcag ctcgttggca agcgcggcgc cgctgtcgtg ggcgaggcgg acgctggcgc
     1501 cgcctttgct ttccagagtg aaggcggccc gggcgccgct cggcacgttg gcgctgaagg
     1561 cgctggcggc gtaatagctc aggcgttgtc cccagggatc gagttcggcg actcccagcg
     1621 ttcgccaggg caggacgcca tgttcgagcg ggcaggcttc gcggccagcg gcttcttcac
     1681 tgctggccag cggcgcttcg gccgggcagg gcaggcggcc gttggtgatg gcaaagccgt
     1741 aaagggcttc gagcgcctcg tcgagttgct gctgtgcccg gcgttcgtca gccagccgac
     1801 gctggccgct cagcgagccg accatgccgc tggcgagcaa gccgaagaca atcaggacga
     1861 tgctcagctc gatcagcgaa aacccgcgct gggccaggcg ttcagtaagc
//
LOCUS       DAOSDX010000620         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20578_length_1909_cov_3.039374, whole genome shotgun sequence.
ACCESSION   DAOSDX010000620 DAOSDX010000000
VERSION     DAOSDX010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20578_length_1909_cov_3.039374"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLN02_12290"
     CDS             <1..187
                     /locus_tag="PLN02_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HOI53150.1"
                     /translation="VLEPEYGIVAIGSGGAYAQSAARALVENTELSPLAIVTKSLEIA
                     GDLCIYTNRNFTLETLE"
     gene            199..1527
                     /gene="hslU"
                     /locus_tag="PLN02_12295"
     CDS             199..1527
                     /gene="hslU"
                     /locus_tag="PLN02_12295"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOI53151.1"
                     /translation="MTPKEIVSELDKHIVGQHAAKKAVAIALRNRWRRAQVAEPLRQE
                     ITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGRDVETIIRDLVE
                     MAIKGHRERAMKANRARAEDAAEERILDALLPPPRSPGFYAEESASSSDNTTRQKFRK
                     KLREGELDDKEIDIELAAPSMQAEIFAPPGMEELTQQIQGMFQNVGGGKKKSRKLKIR
                     EASRLLIDEEAAKLVNDEEIKLEAVKAVEQNGIVFLDEIDKVASRSDVQGADVSRQGV
                     QRDLLPLVEGTTVSTKYGMIKTDHILFIASGAFHLSKPSDLIPELQGRFPIRVELDSL
                     SVADFECILTQTDACLTKQYQALLATEGVTVDFAADGIRRMAEIAYQVNEKTENIGAR
                     RLHTVMEKLLEEVSFEAGKVGLENIAVDAAYVNDKLGEVAADEDLSRYVL"
     gene            1601..>1909
                     /locus_tag="PLN02_12300"
     CDS             1601..>1909
                     /locus_tag="PLN02_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOI53152.1"
                     /translation="MDESSLTFRLLAILFPIFGIVAAGFFYGRKHKPEMAVANRLNMD
                     VFVPALVFAAMAGKSFDLTAYAPLALGGFIVLAVCGLLAWPIARLCGVQPKTLAPPMM
                     F"
BASE COUNT          378 a          604 c          609 g          318 t
ORIGIN      
        1 cgtgctcgaa ccggaatacg gcatcgtcgc catcggttcc ggcggcgcct acgcgcagag
       61 cgcggcacgg gcgttggtcg agaacaccga attgagtccg ctggcgatcg tcaccaagtc
      121 gctggaaatc gccggcgacc tgtgcatcta caccaatcgc aacttcacgc tggagacgct
      181 ggaatgagtg gcgccacgat gaccccgaag gagatcgtct ccgaactcga caagcacatc
      241 gtcggccagc acgcggccaa gaaggcggtg gcgatcgccc tgcgcaaccg ctggcggcgc
      301 gcccaggtgg ccgagccgtt gcgccaggaa atcacgccga agaacatcct gatgatcggc
      361 ccgaccggcg tcggcaagac cgagattgcc cgccgcctgg cgcggttggc cgatgcaccc
      421 ttcatcaagg tcgaggcgac caaattcacc gaggtcggct acgtcggccg cgacgtcgag
      481 acgatcatcc gagatctcgt cgagatggcg atcaaggggc accgcgagcg ggccatgaag
      541 gccaaccgcg cgcgcgccga ggacgccgcc gaggaacgca tcctcgacgc gctactgccg
      601 ccgccacgca gcccaggttt ctacgccgag gagtcggcga gcagcagcga taacactacc
      661 cgccagaaat tccgcaagaa attgcgcgag ggcgaactcg acgacaagga aatcgatatc
      721 gagcttgccg cgccgtcgat gcaggccgaa atcttcgccc cgcctggcat ggaggagctg
      781 acccagcaga ttcaggggat gttccagaat gtgggcggcg gcaagaaaaa atcgcgcaag
      841 ttgaagatcc gggaagccag caggctgttg atcgacgagg aagccgcgaa gctggtcaac
      901 gacgaggaaa tcaagctgga agcggtgaag gcggtcgagc agaacggcat cgtctttctc
      961 gacgaaatcg acaaggtggc cagccgctcc gacgtgcagg gcgccgacgt ctcgcgccag
     1021 ggcgtgcagc gcgacttgct gccgctggtc gaagggacga ccgtttccac caagtacggg
     1081 atgatcaaga ccgatcacat cctgttcatt gcttccggcg ccttccacct gtccaagccg
     1141 tccgatctga ttcccgaact gcagggccgt ttcccgatcc gcgtcgagtt ggattcgctg
     1201 tcggtggccg atttcgaatg catcctgacc cagactgacg cctgtctgac caagcaatac
     1261 caggcgttgc tggcgaccga gggggtgacc gttgacttcg ccgccgacgg tatccggcgg
     1321 atggccgaga ttgcctacca ggtcaatgag aagaccgaga atatcggcgc ccgccgcctg
     1381 catacggtga tggaaaagct gctggaagaa gtgtccttcg aggccggcaa ggtcgggctg
     1441 gaaaacatcg cggtcgatgc tgcctacgtc aatgacaagc tcggcgaagt ggcggccgac
     1501 gaggacctct cccgttacgt gctctaaggg catttccgga tgggcgggcg gccgcgctgc
     1561 ccgcacaatc cgcttcccca tttcttgctg aggccgaacc gtggacgaaa gcagcctgac
     1621 cttccgcctg ctggccatcc tcttcccgat cttcggtatc gtcgccgccg ggtttttcta
     1681 cggccgcaag cacaagccgg aaatggcggt ggccaaccgg ctgaacatgg atgttttcgt
     1741 gccagcgctg gtctttgcgg cgatggccgg caagtccttc gacctcaccg cctacgcgcc
     1801 gctggcgctc ggcggtttca tcgtgcttgc cgtatgcggc ctgctcgcct ggccgatcgc
     1861 ccggctgtgc ggcgtgcagc cgaagacgct ggcgccgccg atgatgttc
//
LOCUS       DAOSDX010000621         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20594_length_1908_cov_2.868322, whole genome shotgun sequence.
ACCESSION   DAOSDX010000621 DAOSDX010000000
VERSION     DAOSDX010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20594_length_1908_cov_2.868322"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLN02_12305"
     CDS             <1..316
                     /locus_tag="PLN02_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOI53153.1"
                     /translation="GMVREVGVMIAAVVASVGLMMLFAGAIGRFVSEHPTIKMLALSF
                     LVVVGVVLIADGFGHHVPKGYIYFAMAFSVCVEMLNIRLRKNAAKPVDLREPYVREEG
                     EG"
     gene            320..973
                     /gene="hda"
                     /locus_tag="PLN02_12310"
     CDS             320..973
                     /gene="hda"
                     /locus_tag="PLN02_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HOI53154.1"
                     /translation="MRQLLLDLLPESAPNLDNFVAGPNAETVAALTGWLAGSPADTAF
                     CLHGGAGCGRSHLLLASGFAYHDAATDPALCGLGDAMQVAVDNVEALDDAGQIALFNC
                     FNRLKMAGGRLLVAAAQPPAHLALREDLRTRLGSGLIYRLQALSDAEKAAALATQARE
                     RALKLPPEAIDYLLRHAPRDMRWLSAFIVALDQHTLEHKRAVTLPLLRELLNHESPA"
     gene            970..1638
                     /locus_tag="PLN02_12315"
     CDS             970..1638
                     /locus_tag="PLN02_12315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOI53155.1"
                     /translation="MNLALFDLDNTLIAGDSDFEWAQFLIAKGVVDREIQEAKNVEFY
                     EHYKAGTLDIGAFLEFQLAPLTRHSRQELDAWHAEFMDRHIRPIIGEPARALVREHLD
                     AGDLCAIVTATNSFVTGPIAREFGIPHLIGTIPAVDLKDGTFFGTPTGTPSFQAGKIV
                     RVEQWLEALGLWWGSFETTSFYSDSHNDLPLMKQVKMPVAVDPDDRLHAYASEMGWKI
                     ITLR"
     gene            1687..>1908
                     /locus_tag="PLN02_12320"
     CDS             1687..>1908
                     /locus_tag="PLN02_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOI53156.1"
                     /translation="MIRKLISRVFSGKNNRAGRNEPVVIPVSGHGITRDRISSGSRRT
                     CETLQAEGFKAYVVGGAVRDLLIGAEPKDF"
BASE COUNT          322 a          697 c          572 g          317 t
ORIGIN      
        1 cggcatggtg cgcgaggtcg gcgtgatgat cgccgccgtc gtcgcctcgg tcggcctgat
       61 gatgctcttt gccggggcca tcgggcgctt tgtttccgag cacccgacga tcaagatgct
      121 ggccctctcc ttcctcgtcg tcgtcggcgt cgtgctgatt gccgacggtt tcggccacca
      181 cgtgccgaag ggctacatct acttcgcgat ggctttctcg gtctgcgtcg agatgctcaa
      241 catccgcctg cgcaagaacg ccgccaagcc ggtggacctg cgcgagccct acgtgcgcga
      301 agaaggcgaa ggctgagcga tgcgccagtt gctgctcgac ctgctgccgg aaagcgcgcc
      361 caacctggac aatttcgtcg ccggccccaa cgccgagacg gtcgccgcgc tcaccggctg
      421 gctcgccggc tcgcccgccg acaccgcctt ctgcctgcac ggcggcgccg gttgcggacg
      481 cagccacctg ctactggcga gcggtttcgc ctaccacgat gcggccaccg acccggcgct
      541 gtgcgggctt ggcgacgcca tgcaggtggc ggtcgataac gtcgaggcgc tcgacgacgc
      601 cggccagatc gcgctgttca actgtttcaa ccgcttgaag atggccggcg gccgcttgct
      661 cgtcgccgcc gcccagccac ccgcccacct ggcgctgcgc gaagacctgc gcacccgcct
      721 gggctccggc ctgatctacc gcctgcaagc cttgagcgac gccgaaaagg cggcggcgct
      781 ggccacccag gcgcgcgaac gggcgttgaa gctgccgccg gaagcgatcg attacctgct
      841 gcgccacgcg ccgcgcgaca tgcgctggct gtccgccttc atcgtcgccc tcgaccagca
      901 caccctcgaa cacaaacgcg ccgtcaccct gccgctgctg cgcgaactgc tcaaccacga
      961 aagccccgca tgaacctcgc cctctttgac ctggacaaca ccctgatcgc cggcgactcc
     1021 gatttcgaat gggcgcagtt cctgatcgcc aagggcgtcg tcgaccgcga gatccaggaa
     1081 gccaagaacg tcgagttcta cgagcactac aaggccggca cgctcgacat cggcgctttc
     1141 ctcgaattcc agctggcgcc gctgacccgc cattcgcgcc aggaactcga cgcctggcac
     1201 gcggaattca tggaccgcca catccgcccg atcatcggcg agccggcgcg cgccctggtc
     1261 cgcgaacatc tagacgccgg cgatctgtgc gccatcgtca ccgccaccaa cagtttcgtc
     1321 accggcccga tcgcccgcga attcggcatc ccgcacctga tcgggacgat tccggcggtc
     1381 gatctgaagg atggcacctt cttcggcacg ccgaccggca caccgtcgtt ccaggcgggc
     1441 aagatcgtcc gcgtcgaaca atggctggaa gcgctcggcc tgtggtgggg cagcttcgag
     1501 accaccagct tctacagcga ttcgcacaac gacctgccgc tgatgaagca ggtgaaaatg
     1561 ccggtcgccg tcgacccgga cgacaggctg cacgcttacg cgagcgaaat gggctggaaa
     1621 atcatcactt tgcggtaaaa tctccgtctt tccgtcgcgc cccatgcgcc tgcccctcag
     1681 gaactcgtga tccgcaaact catttcccgc gttttcagcg gcaagaacaa ccgcgccggc
     1741 cgcaacgagc cggtcgtcat cccggtctcc ggccacggca tcacccgcga ccgtatctcc
     1801 agcggtagtc ggcgcacctg cgagaccctg caggccgaag gcttcaaggc ctacgtcgtc
     1861 ggcggtgccg tgcgcgacct gctgatcggc gccgaaccga aggatttc
//
LOCUS       DAOSDX010000622         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20602_length_1907_cov_4.265659, whole genome shotgun sequence.
ACCESSION   DAOSDX010000622 DAOSDX010000000
VERSION     DAOSDX010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20602_length_1907_cov_4.265659"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLN02_12325"
     CDS             complement(<1..346)
                     /locus_tag="PLN02_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI53157.1"
                     /translation="MNAPDHPAAQGKIMLVGLGPGSHDHLTARARAAIAEADTIIGYV
                     TYIRLIADLVEGKEVIKKSMTEELDRAVEALDRARQGKKVALISSGDAGVYGMAGPTF
                     EVLFAAGWQPEGD"
     gene            complement(333..737)
                     /locus_tag="PLN02_12330"
     CDS             complement(333..737)
                     /locus_tag="PLN02_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HOI53158.1"
                     /translation="MKITLGLGCDRNTPASTIARCIEEALAACGRTPADVRAAASIDL
                     KADEPGLLEVIARHGWPALFYPASELAAVDVANPSETVRKYTGTPSVSEAAALLAAGA
                     DKSHLLIEKHRLRGPDGRNATVSIARIPHERT"
     gene            complement(1315..>1907)
                     /locus_tag="PLN02_12335"
     CDS             complement(1315..>1907)
                     /locus_tag="PLN02_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis central domain-containing
                     protein"
                     /protein_id="HOI53159.1"
                     /translation="CYAGKVGEQVPALFAAFDGIVAIVSLGAVVRLIAPHLKGKEVDP
                     AVVVIDEAGKFAIPVLSGHLGGANQLAGHLAAALGAQAVLTTASDARETIGVDILGRE
                     LGWTFEATHDELVRCSAAMVNDEPVALIQEAGSPDWWANHANGRNGPLPANVSLFARL
                     EDVDPERFAAVLWISKRAMPASYAAQLAGRHVIYRP"
BASE COUNT          264 a          605 c          690 g          348 t
ORIGIN      
        1 cgtcgccctc gggctgccaa ccggcggcga agagcacctc gaaggtcggc ccggccatgc
       61 cgtagacgcc ggcatcgccc gaggaaatca gcgccacctt cttgccctgg cgggcgcggt
      121 cgagcgcttc gacggcgcgg tccagttctt cggtcatcga cttcttgatc acctccttgc
      181 cttcgaccag gtcggcgatc aggcggatgt aggtgacgta gccgatgatc gtgtcggcct
      241 cggcaatcgc cgcgcgggcg cgcgccgtca ggtggtcgtg gctgcccggg ccgaggccga
      301 cgagcatgat cttgccttgg gcggcgggat ggtcaggtgc gttcatgggg aatcctggca
      361 atggagacgg tggcgttgcg gccgtccggg ccgcgcagcc gatgtttttc gatgagtagg
      421 tgacttttgt cggcgccggc agcgagcagc gccgccgctt ccgagacgga aggcgtgccg
      481 gtgtacttgc gtaccgtttc cgacggattg gccacgtcga ccgcggccag ttcgctggcc
      541 gggtagaaca gcgccggcca gccgtggcgg gcaatgactt caaggaggcc gggttcgtcg
      601 gccttgaggt cgatactggc tgccgctcgc acgtcggccg gggtacgtcc gcaggccgcg
      661 agcgcttctt cgatgcagcg ggcgatggtg ctggcggggg tgttgcggtc gcagccgagg
      721 ccgagggtga ttttcaagat ttcgttccct tcacctgtgc cgagcggtgc gtgtcgggct
      781 gtaacgagcg gcgttccgtt tcgaccgccg ttttccgccg ttgaccggcg tcttcctttc
      841 tttgcttcgc caaagaaagg aagcaaagaa aggcgacccc cacgttcgcg ccgggccgtc
      901 ggcccggttc cctgcgctgc tcgaccggcc gggcggttcg caaaactcgc ctgcggctca
      961 aacattgcga accgactgcc cccggccggc ctgcgctgct cggcgctcgc gaggggggag
     1021 gtggcgctcc gtggctgcgt atgcggtggt ttttcatggg cgatagatca cgcccggcgt
     1081 ctctcgcatt ttgcttcgtc aaaaaacgga agcaaaaaaa gctgactccc acgttcgcgc
     1141 cgggccgtcg gcccggttcc ctgcgctgct cgaccggccg ggcggttcgc aaaactcgcc
     1201 tgcggctcgg acattgcgaa ccgactgccc ccggccgatc tgcgctgctc ggcgctcgcg
     1261 aggggggaag tggcgctccg tggctgcgta tgcggttgcg catgcggtgg cctttcatgg
     1321 gcgatagatc acgtggcggc cggccagttg tgcggcgtag cttgccggca ttgcccgttt
     1381 gctgatccac agtacggcag cgaaacgctc gggatcgacg tcttcgagac gggcgaacaa
     1441 gctcacgttg gccggcagcg ggccgttgcg gccgttggcg tggtttgccc accagtccgg
     1501 cgagccggct tcctggatca gggcgaccgg ctcgtcgttg accatcgccg cactgcagcg
     1561 caccagttcg tcgtgcgtgg cctcgaaggt ccagccgagt tcgcggccga ggatgtcgac
     1621 gccgatggtt tcgcgcgcgt cggacgcggt ggtcagcact gcctgggcgc cgagcgcggc
     1681 ggcgaggtgg ccggccaact ggttggcgcc gccgaggtgg ccggagagga ccgggatggc
     1741 gaacttgccg gcttcgtcga tgacgaccac ggccgggtcg acttccttgc ccttgagatg
     1801 cggcgcgatc aggcggacga cggcgccgag cgagacgatg gcgacgatgc cgtcgaaggc
     1861 ggcgaacagc gccggcacct gctcgccgac cttgccggcg taacagt
//
LOCUS       DAOSDX010000623         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20639_length_1905_cov_3.144865, whole genome shotgun sequence.
ACCESSION   DAOSDX010000623 DAOSDX010000000
VERSION     DAOSDX010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20639_length_1905_cov_3.144865"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLN02_12340"
     CDS             <1..933
                     /locus_tag="PLN02_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOI53160.1"
                     /translation="GAEQLTQFISALNDALTRRVIQLNLENHDFTGIDWCWLAFGSEG
                     RDEQTFSTDQDNGIVFVVPDGEDKDALKQRFLAFALATNQDLDQVGFPLCKGNIMASN
                     PQWCLTLDEWKAQFSKWIRTPEPVALLNATIFFDFRPLYGKVELPDAMRRHLLDQASA
                     TPIFLQAMARNALEVGPPLGKIRDFVTDLEPDHPGKIDLKKYGSRIFVDVARIYALAA
                     GVHGTNTLQRLRLSAQKMNIRGEEINAVLDGLNFIQLLRLQHQYLEGEPGRQGDNLID
                     PEKLNELDRRILKESFRQARKIQSRLKLDYQVNN"
     gene            936..1601
                     /locus_tag="PLN02_12345"
     CDS             936..1601
                     /locus_tag="PLN02_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53161.1"
                     /translation="MFGIKKLLFGEGASGTLPEDVRNAVNAWSASQGEAFESSHFLTR
                     YVVVDIATTGLDPERDRLTGIAATVLHKGVIAPEQSLCIDLDPAGADPALLDRQLAVF
                     LRFVGKSPLVAYHAAYVAAFLEQLIRKRLGVTFQPRWVDLVWLLPALFGERSVKPLPL
                     DDWLDAFGLDAGEGRRSPIANSLLLARLMQMLVVRAHAKGVDDVAGLIDESKASSHLR
                     RTH"
     gene            1609..>1905
                     /locus_tag="PLN02_12350"
     CDS             1609..>1905
                     /locus_tag="PLN02_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOI53162.1"
                     /translation="MLASLTRLQRYYLYYTGIFFGFIFFLAVLERAGMPPRWVGYAFL
                     VFTVSMYALIGVFSRTTDVSEYYVAGRRVPAFFNGMATGADWMSAASFMGLAGTL"
BASE COUNT          324 a          650 c          587 g          344 t
ORIGIN      
        1 ggcgccgagc aactgaccca gttcatctcg gcgctcaacg atgcgctgac ccggcgcgtc
       61 atccagctca acctggaaaa ccacgatttc accggcatcg actggtgctg gctggctttc
      121 ggctcggagg ggcgcgacga gcagaccttc agcaccgacc aggacaacgg tatcgtcttc
      181 gtcgttcccg acggcgagga caaggacgcg ctgaagcagc gctttctggc tttcgcgctg
      241 gccaccaatc aggacctgga ccaggtcggt ttcccgctgt gcaagggcaa catcatggcg
      301 agcaatccgc agtggtgcct gacgctggac gaatggaagg cgcagttcag caagtggatc
      361 cgcacgccgg agccggtggc gctgctcaac gcgacgatct tcttcgattt ccggccgctc
      421 tacggcaagg tcgaactgcc cgacgcgatg cgccgccatc tgctggatca ggcgtcggcg
      481 acgccgatct tcctgcaggc gatggcgcgc aacgcgctcg aggtcgggcc gccgctgggc
      541 aagattcgcg atttcgtcac cgacctggag cccgatcatc cgggcaagat cgatctcaag
      601 aagtacggct cgcgcatctt cgtcgacgtc gcgcgcatct acgcgctcgc cgctggcgtg
      661 catggcacca acacgctgca gcgcctgcgc ttgtcggcgc agaagatgaa catccgcggc
      721 gaggagatca acgccgtcct cgacggcctc aacttcatcc agttactgcg cctgcagcat
      781 cagtacctcg aaggcgagcc ggggcgccaa ggcgacaacc tgatcgatcc ggaaaagctc
      841 aacgagctgg atcgccgcat cctcaaggaa tccttccgcc aggcacgcaa gatccagtcg
      901 cgcctgaaac tcgactacca ggtgaataac tgatcatgtt cgggatcaag aaactcctgt
      961 tcggggaagg cgccagcggg acgctcccgg aggacgtgcg caacgcggtc aacgcctggt
     1021 cggcaagtca gggcgaagcg tttgaatcct cgcatttcct gacccgttat gtcgtcgtcg
     1081 atatcgccac caccggcctc gaccccgagc gcgaccgctt gaccgggatc gccgcgaccg
     1141 tcctgcacaa gggcgtcatc gccccggagc agagcctgtg catcgacctc gacccggcgg
     1201 gcgccgaccc ggcactgctc gaccggcaac tggcggtttt cctgcgcttc gtcggcaagt
     1261 cgccgctggt cgcctaccat gccgcctacg tcgcggcctt tctggaacag ttgatccgca
     1321 agcgcctcgg cgtcactttc cagccacgct gggtcgatct ggtctggctg ttgccggcgc
     1381 tgttcggcga gcgcagcgtc aagccgttgc cgctcgacga ttggctcgac gccttcgggc
     1441 tcgacgccgg cgaagggcgg cgcagtccga tcgccaacag cctgttgctg gcgcgcctga
     1501 tgcagatgct ggtggtacgc gcgcacgcca agggggtcga tgacgtcgcc gggctgatcg
     1561 acgaatcgaa ggccagcagc cacctgcgcc gtacccactg acgccggtat gctcgcctcc
     1621 ctgacccggc tgcagcgtta ctacctctac tacaccggga ttttcttcgg cttcatcttt
     1681 ttcctggctg tgctcgaacg cgccggcatg ccgccgcgct gggtcggcta cgccttcctg
     1741 gtgttcaccg tgtcgatgta cgcgctgatc ggcgtcttca gccggaccac cgacgtttcc
     1801 gagtattacg tcgccggccg ccgggtgccg gccttcttca acggcatggc gaccggcgcc
     1861 gactggatga gcgccgcctc cttcatgggg ctggccggca ccctc
//
LOCUS       DAOSDX010000624         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20642_length_1905_cov_2.807568, whole genome shotgun sequence.
ACCESSION   DAOSDX010000624 DAOSDX010000000
VERSION     DAOSDX010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20642_length_1905_cov_2.807568"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..466
                     /locus_tag="PLN02_12355"
     CDS             116..466
                     /locus_tag="PLN02_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53163.1"
                     /translation="MNNMQSPKILPWIAKRAGISEELALKLWRRAVGEAETLTGQSSG
                     AEFSGLAIERFLAIVEDEADTQSAAKLSPAPQLSWLWRHQTRMSLLSLMAAQNTYQFW
                     QNTWNDMVGQKKAA"
     gene            639..>1905
                     /gene="leuS"
                     /locus_tag="PLN02_12360"
     CDS             639..>1905
                     /gene="leuS"
                     /locus_tag="PLN02_12360"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041224677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOI53164.1"
                     /translation="MQEKYTPADLERAAQQHWDETGAARAVEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFHKMQGYNVLQPMGWDAFGMPAENAAMQNNVAPAGWTY
                     SNIDYMRSQLKSLGFAIDWQREFATCTPEYYRWEQWLFTRLYEKGLVYKKLGTVNWDP
                     VDHTVLANEQVIDGKGWRSGAVVEKREIPMYYMKITAYAEELLAELDNLPGWPEQVRL
                     MQKNWIGKSTGVRFAFPLADNADEKLWVFTTRADTIMGVTFVAVAAEHPLASRAAANN
                     PELAAFIEECKQGGVAEADLATMEKKGMPTGIYVSHPLTGEQVEVWVGNYVLMSYGDG
                     AVMAVPAHDERDFAFALKYQLPIKQVVEAGIFRELKLGLSEPALKEAGREQLQEALEK
                     EAFSAEEWKAWYADKNGVCINSGKYDGLGY"
BASE COUNT          429 a          623 c          566 g          287 t
ORIGIN      
        1 cagccggggt gccaaaagta tactggcagc aaatatattg cagtgcacaa aactatttgc
       61 ccacgaggca gtcgaatgga atggggtcac caccgtgacc gacaaggagc agcaaatgaa
      121 caacatgcaa tcaccgaaaa tcctcccctg gatcgccaag cgcgccggca tcagtgaaga
      181 actggcgctc aagctgtggc gtcgcgccgt cggcgaagcc gaaaccctga ccggccaatc
      241 gtccggcgcc gagttctccg gcctggccat cgagcgcttc ctggccatcg tcgaagacga
      301 agccgacacc cagtccgccg ccaagctgtc gccggccccg caactgagct ggctctggcg
      361 tcaccagacc cggatgtcgc tgctgtcgct gatggccgcg cagaatacct accagttctg
      421 gcagaacacc tggaacgaca tggtcggcca aaaaaaagcc gcctgaccgg acgtcgctga
      481 cgttcggccc gttgcgccgc gagccggcgg cgtgacgggg aaccgcccgg cacgtcacgc
      541 cctgcgcgcg cgggaaatgc tgaaaaccgg cgccgatacc ggggcgcgca accgctataa
      601 ttcgcggttt gatcgacgct cattcagctc acacgcccat gcaggaaaaa tacaccccgg
      661 ccgacctcga acgcgccgcc cagcaacact gggatgaaac cggcgccgcc cgcgccgtcg
      721 aagacgccag caaacccaag tactactgcc tgtcgatgtt cccgtatccg tccgggaaac
      781 tgcacatggg ccatgtgcgc aactacacga tcggcgacgt cctctcgcgc ttccacaaga
      841 tgcagggcta caacgtgctg cagccgatgg gctgggacgc cttcggcatg ccggcggaaa
      901 acgcggccat gcagaacaat gtcgcgccgg ccgggtggac ctactcgaac atcgattaca
      961 tgcgctcgca gctcaagtcg ctcggcttcg ccatcgactg gcaacgcgaa ttcgccacct
     1021 gcacgcccga gtactaccgc tgggaacaat ggctgttcac ccgcctctac gaaaagggcc
     1081 tggtgtacaa gaagctcggc accgtgaact gggacccggt cgaccacacc gtgctcgcca
     1141 acgaacaggt catcgacggc aagggctggc gctccggcgc cgtcgtcgaa aagcgcgaga
     1201 tccccatgta ctacatgaag atcaccgcct acgccgaaga actgctcgcc gaactcgaca
     1261 atctgccggg ctggccggaa caggtgcgcc tgatgcagaa gaactggatc ggcaagagca
     1321 ccggcgtgcg cttcgccttc ccgctggcgg ataacgccga cgaaaagctg tgggtgttca
     1381 ccacccgcgc cgacaccatc atgggcgtca ccttcgtcgc cgtcgccgcc gaacacccgc
     1441 tggccagccg cgctgccgcc aacaacccgg aactcgccgc cttcatcgag gaatgcaagc
     1501 agggcggcgt cgccgaggcc gatctggcga cgatggaaaa gaaggggatg ccgaccggca
     1561 tctacgtcag ccatccgctg accggcgaac aggtcgaagt ctgggtcggc aactacgtgc
     1621 tgatgagcta cggcgacggc gccgtgatgg ccgtgccggc gcacgacgag cgcgatttcg
     1681 cgtttgcgct caagtaccaa ctgccgatca agcaggtggt tgaagcaggc atttttagag
     1741 aactcaagtt gggtctaagc gagcccgccc tgaaagaagc cggacgcgag caactgcaag
     1801 aggctcttga gaaagaagcc ttttctgcgg aagagtggaa agcttggtac gcagacaaaa
     1861 acggggtttg catcaactcc ggcaaatacg acggcctcgg ctacg
//
LOCUS       DAOSDX010000625         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20645_length_1905_cov_2.327568, whole genome shotgun sequence.
ACCESSION   DAOSDX010000625 DAOSDX010000000
VERSION     DAOSDX010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20645_length_1905_cov_2.327568"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLN02_12365"
     CDS             complement(<1..590)
                     /locus_tag="PLN02_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOI53165.1"
                     /translation="MNAQDLAHLIPLSDLSNAALTQACALARPFIAEPGQLVFPQKSA
                     DAHSYYLLSGEVALDREDGSAPTRLSAGTPAARHPLARQKPRAFSCRAMCRSLLVVFG
                     DDDLDHLLTRDQATAYEVTEFEGDDPQWMFDVLKNPAFSKVPPGNLHRLFGRFEALPV
                     AAGQVVVRQGEPGDHYYLIRSGSARVTRRAGEQKEIV"
     gene            complement(713..>1905)
                     /locus_tag="PLN02_12370"
     CDS             complement(713..>1905)
                     /locus_tag="PLN02_12370"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOI53166.1"
                     /translation="DPDDPFFQFFRQFRGMVPQDNTPVRGLGSGFIVSPDGTVLTNAH
                     VVADADEVIVKLNDKREFKAKILGMDPASDVAVLKIDGKNLPTVRVGSAKASQVGEWV
                     LAIGSPYGFESSASAGIISAKSRSLPDGSYVPFLQTDVAVNPGNSGGPLFNMRGEVIG
                     INSQIYSRSGGYQGLSFAIPIEVAMNVERQIVAHGKVERGRLGVAIQEVDQALADSFG
                     LARPAGALVSSVDKDGPAGRAGLEAGDVILGIDGQAVEISGELPSIIAAKRPGETVRL
                     QIWRNKAARDITVKVGAFQDDKVAAAEPAKADKGRLGVAVRPLTPEERRQSGIAGGLL
                     VEQAGGAAARAGIRPGDVILSINGEAVNDTEQLRQLIGKSGKKVALLIERGKARLFVP
                     VELG"
BASE COUNT          286 a          600 c          696 g          323 t
ORIGIN      
        1 acgatttcct tctgctcgcc ggcacgacgg gtgacgcgcg cgctgccgga gcggatcagg
       61 tagtagtggt cgccgggctc gccctggcgg acgacgacct ggccggcggc aaccggcagc
      121 gcttcgaagc ggccgaagag acgatgcagg ttgcccggcg gcaccttgct gaaggccgga
      181 ttctttagca cgtcgaacat ccattgcgga tcgtcgcctt cgaactcggt gacttcgtag
      241 gcggtggcct ggtcgcgcgt cagcaggtgg tcgaggtcgt cgtcgccgaa gaccaccagc
      301 agcgagcggc acatcgcccg gcaactgaag gcgcgcggct tctggcgcgc cagcgggtgc
      361 cgggcggcag gcgtgccggc gctcaggcgg gtcggcgccg aaccgtcctc gcggtcgagc
      421 gcgacttcgc cggagagcag atagtagctg tgggcatcgg cgcttttctg cgggaagacg
      481 agttgcccgg gttcggcgat gaaggggcgg gccagtgcgc aggcctgggt cagggcggcg
      541 ttggagaggt cggagagagg gatcaggtgg gcgaggtctt gcgcgttcat gatgggcgtg
      601 gcagccgcag gcgacgccgt gattctacgg caatgcccag ctgacatggc cgccacccag
      661 caaaaaggag ccagcttgcg ccggcccctg tgtcattttt cgcgctgccg cgtcagccca
      721 gttcgaccgg cacgaaaagc cgggccttgc cgcgctcgat cagcagggcg accttcttgc
      781 cggatttgcc gatcaactgg cgcaactgct ccgtatcgtt caccgcctcg ccgttgatcg
      841 acaggatcac gtcgcctggc cggataccgg cacgggcggc ggcaccaccc gcctgttcga
      901 cgaggaggcc gccggcgatg ccgctttgcc ggcgctcttc cggggtcagc ggacggacgg
      961 cgacgccgag gcggcccttg tcggccttgg ccggttcggc ggcggcgacc ttgtcgtcct
     1021 ggaaggcgcc gaccttcacc gtgatgtcac gtgccgcctt gttgcgccag atttgcaggc
     1081 gcaccgtttc gcccggtcgc ttggcggcga tgatcgacgg cagttcgccg gaaatctcca
     1141 ctgcctggcc gtcgatgccg aggatcacgt cgcccgcttc gaggccggcg cggccggccg
     1201 gaccgtcctt atcgaccgag gagacgaggg cgccggcggg acgggcgagg ccgaagctgt
     1261 cagccagcgc ctggtcgact tcctggatgg cgacgccgag gcggccgcgc tcgaccttgc
     1321 cgtgggcgac gatttgtcgc tcgacgttca tcgccacctc gatcgggatg gcgaaggaga
     1381 ggccctggta gccgccgctg cgcgagtaga tctgcgagtt gatgccgatc acttcgccgc
     1441 gcatgttgaa cagcggcccg ccggaattgc cgggattgac cgcgacgtcg gtctgcagga
     1501 aaggaacgta gctgccgtcc ggcaagctgc gcgacttggc ggagatgatg ccggcactgg
     1561 cgctcgattc gaagccgtag ggcgagccga tggcgagcac ccattcgccg acctgcgacg
     1621 ccttggcgct gccgacgcgc accgtgggca ggttcttgcc gtcgatcttg agcacggcta
     1681 cgtcgctggc cgggtccatg ccgaggatct tggccttgaa ttcgcgcttg tcgttgagct
     1741 tgacgatcac ttcgtcggcg tcggcaacga cgtgggcgtt ggtcagcacg gtaccgtcgg
     1801 ggctgacgat gaagccggag ccgaggccgc gtaccggcgt gttgtcctgc ggcaccatgc
     1861 cgcggaactg gcggaagaac tggaagaagg gatcgtcggg gtcga
//
LOCUS       DAOSDX010000626         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20696_length_1901_cov_2.697183, whole genome shotgun sequence.
ACCESSION   DAOSDX010000626 DAOSDX010000000
VERSION     DAOSDX010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20696_length_1901_cov_2.697183"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..>1059)
                     /locus_tag="PLN02_12375"
     CDS             complement(18..>1059)
                     /locus_tag="PLN02_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOI53167.1"
                     /translation="RQRRHNPFPIVCGTSAGAINAVGLACQAEHFGRATTTLARFWRD
                     LRAADIYRSDALGVGAAGARWLGALTLGWLVGRAPRSLLDNAPLATLLGRSMDFAGIE
                     RAIASGALRAISISASGYESGENINFFQAHPDAAPWHRFQRIGVRTQLGVEHLLASSA
                     IPFIFPATRINREYFGDGSMRQLAPLSPAIHLGAERLLVVGATRGNEKRERRQVDDYP
                     SLAQIAGHALASIFVDGLAVDIERIQRINRTVSAIPEALRAQSGIALRPIETLIISPS
                     ESLDRIAAEHADALPWPIRLLLGGIGAMNRQGGALTSYLLFEKPFTRKLIELGHADTL
                     ARGDEVGRFLDL"
     assembly_gap    1060..1069
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1166..1618)
                     /gene="queD"
                     /locus_tag="PLN02_12380"
     CDS             complement(1166..1618)
                     /gene="queD"
                     /locus_tag="PLN02_12380"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOI53168.1"
                     /translation="MLITRRLEFDAGHRIPDHKSQCRHLHGHRYAIEITLSGNIIDKA
                     GDAANGMVMDFSQVKELAMTHLVGRWDHAFLVYEGDRAIVDFLASLPDHKTVVLDRVP
                     TAENLARLAFDCLDAVYRDTYGNHLRLEQVRLYETPNCWADATRQAQD"
     gene            1666..>1901
                     /locus_tag="PLN02_12385"
     CDS             1666..>1901
                     /locus_tag="PLN02_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOI53169.1"
                     /translation="MLRTDDHRRDSAGLRYVYPVISRRAGGVSVGINLNPNNACNWAC
                     VYCQVEGLTRGGPPPLELDRLEAELAGFLDDAING"
BASE COUNT          296 a          591 c          695 g          309 t
ORIGIN      
        1 cgatgccagc acctgagtca gagatcgagg aagcgaccga cctcgtcgcc acgcgccagc
       61 gtgtcggcgt ggccgagttc gatcagtttg cgcgtgaaag gcttctcgaa cagcaggtag
      121 ctggtcagcg cgccgccctg ccggttcatc gcgccgatcc cgccgagcag caggcggatc
      181 ggccagggca gggcgtcggc gtgctcggcg gcgatgcggt cgaggctttc cgacggcgag
      241 atgatcagcg tctcgatcgg ccgcagcgcg attcccgact gtgcccttag cgcttccggg
      301 atcgccgaga cggtgcggtt gatgcgctgg atgcgttcga tgtcgacggc cagcccatcg
      361 acgaagatgc tcgccagcgc gtgtccggcg atctgcgcca gcgacggata gtcgtcgacc
      421 tggcggcgtt cgcgcttttc gttgccgcgc gtcgcgccga ccaccagcag ccgttcggcg
      481 cccaggtgga tggccggcga cagcggtgcc agctggcgca tcgagccgtc gccgaaatac
      541 tcgcggttga tccgcgtcgc cggaaagatg aagggaatcg cgctcgacgc cagcaggtgt
      601 tcgacgccga gctgcgtgcg cacgccgatg cgctggaagc ggtgccaggg cgcggcgtca
      661 ggatgcgcct ggaagaaatt gatgttctcg cccgattcgt aaccggacgc cgaaatgctg
      721 atggcacgta gggcgccgct ggcgatcgcg cgttcgatgc cggcgaagtc catgctgcgc
      781 ccgagcaggg tggcgagcgg cgcgttgtcg agcaacgagc gcggcgcccg gccgaccagc
      841 cagcccaggg tcagggcccc cagccagcgg gcgccggcgg cgccgacacc gagcgcgtcg
      901 ctgcgataga tgtcggcggc gcgcaggtcg cgccagaaac gcgccagggt ggttgtcgcc
      961 cggccgaaat gttcagcctg gcaggccagc ccgacggcgt tgatggcgcc ggcagaagtg
     1021 ccgcagacga tgggaaaggg attgtgacgg cgctgccggn nnnnnnnnnc aagtttggcg
     1081 accgcggtga ggacgccgac ctggtaggcc gcccgggcgc cgccgccggt caggaccagc
     1141 gctgagcctt ggcgggccgg ggaagtcaat cctgcgcctg ccgggtcgcg tcggcccagc
     1201 aattgggggt ttcgtagagg cgcacctgtt cgagccgcag gtggttgccg taggtgtcgc
     1261 ggtacaccgc gtcgaggcag tcgaaggcga ggcgggcgag gttctcggcg gtcggcacgc
     1321 ggtcgaggac gaccgtcttg tggtccggga gactggccag gaaatcgacg atcgcgcggt
     1381 cgccttcata gaccaggaag gcgtggtccc agcggccgac cagatgggtc atcgccagtt
     1441 ccttgacctg cgagaagtcc atcaccatgc cgttggcggc gtcgccggcc ttgtcgatga
     1501 tgttgccgga aagggtgatc tcgatcgcgt agcggtggcc gtgcaggtgg cggcactggc
     1561 tcttgtggtc ggggatgcgg tggcctgcgt cgaattccag gcggcgggtg atcaacattt
     1621 gggattaggg ttcgccaaga cgggggctga ttatacaatg cccccatgct gcgaaccgac
     1681 gaccaccgcc gcgacagtgc cggcctccgc tatgtttatc ccgtgatttc ccggcgcgcc
     1741 ggcggcgtct cggtcggcat caacctcaat cccaacaacg cctgcaactg ggcctgcgtc
     1801 tattgccagg tcgaaggatt gacccgcggc ggtccgccgc cgcttgagct cgatcggctg
     1861 gaggcggagc tggctggctt tctcgacgat gcgatcaacg g
//
LOCUS       DAOSDX010000627         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20871_length_1889_cov_2.622683, whole genome shotgun sequence.
ACCESSION   DAOSDX010000627 DAOSDX010000000
VERSION     DAOSDX010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20871_length_1889_cov_2.622683"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /gene="murI"
                     /locus_tag="PLN02_12390"
     CDS             complement(<1..759)
                     /gene="murI"
                     /locus_tag="PLN02_12390"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOI53170.1"
                     /translation="MNSRPIAVFDSGLGGLTVLRALRARLPHEDYCYFADTRFLPYGD
                     KPESFLRERGVRIAAALAGRGAKALVIACNTATAAAAEAIRAATTLPVVALEPGVKPA
                     AALSRSGVVGVLATTRTLASERFQRLCTSHASHLRVLPQACPGLADAIEMHGADSIEV
                     EALLDRFVPPLGAADADVVVLGCTHYPWVAAAIARRLPPGVELLDTGEAVARQLERLL
                     AGQGLVGGGAGHLKLMTSGAPLQVEATVERLLGRK"
     gene            complement(774..1742)
                     /gene="corA"
                     /locus_tag="PLN02_12395"
     CDS             complement(774..1742)
                     /gene="corA"
                     /locus_tag="PLN02_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOI53171.1"
                     /translation="MLINCVAYENGTRLNEIAVEEISDYLERPGCFVWVALRDATTEE
                     LDIMQEEFHLHELAVEDARKGHQRPKVEDYGDSIFAVMHLIEPDVDEGYRLGEVNVFV
                     GPNYVLSVRNRSQQDFLGVRARCEREPHLLQHGSGFVLYALMDAVVDRYFPVVDRLES
                     ELESIEDRIFDRGTARANIERLYDLKRQVMQLKHVVAPLMEVAGKLASNRGPALCSAN
                     REYFRDVYDHLLRIDASVDTIRETIGTAIQVNLSMVAIEDSEVSKRLAAWAGIFAIAT
                     AFAGIWGMNFAQMPELQWEYGYPAALTLIVSTCLILYRRFKRAGWL"
BASE COUNT          316 a          624 c          653 g          296 t
ORIGIN      
        1 cttcctgccc agcaggcgct cgaccgtcgc ttcaacctgc aagggcgcgc cgctggtcat
       61 cagcttcagg tgaccggcgc cgccgccgac cagcccctgg ccggcgagca ggcgttcgag
      121 ctggcgggcg accgcctcgc cggtgtcgag cagttcgacg ccgggcggca gacggcgggc
      181 aatcgccgcc gccacccagg ggtaatgcgt gcagccgagg acgacgacat cggcgtcggc
      241 ggcacccaat ggcggcacga agcggtcgag caaggcctcg acctcgatac tgtcggcgcc
      301 gtgcatttca atcgcgtcgg ccagcccggg gcaggcctgc ggcagcaccc ggagatgact
      361 ggcgtggctg gtgcaaagac gctggaagcg ttcgctggcc agcgtccgcg tcgtcgccag
      421 gacgccgacg acgccgctgc ggctgagcgc ggcggccggc ttgacgccgg gttcgagggc
      481 gaccacgggc aaggtcgtcg ccgcccggat cgcttcggcg gcggcggcgg ttgccgtgtt
      541 gcaggcgatc accagcgcct tggcgccacg cccggcgagc gccgcggcga tgcgcacgcc
      601 gcgctcgcgc aggaaggact caggcttgtc accgtagggc aggaaacggg tgtcggcgaa
      661 gtaacagtaa tcctcgtgcg gcagccgggc gcgcaaggcg cgcagcacgg tcaggccacc
      721 gaggccggaa tcgaagacgg cgatgggtcg ggagttcacg tcgtggcgag ggcctagagc
      781 cagccggcgc gcttgaagcg tcggtagagg atgaggcagg tcgagacgat cagcgtcagc
      841 gccgccggat agccgtattc ccactgcagt tcgggcatct gcgcgaagtt catgccccag
      901 atcccggcga aggcggtggc gatggcgaag atcccggccc aggcggccag ccgcttgctg
      961 acttcgctgt cctcgatcgc caccatcgac aggttgacct ggatcgccgt gccgatggtt
     1021 tcgcggatcg tatcgaccga ggcgtcaatg cgcagcaagt ggtcatagac gtcgcggaaa
     1081 tactcgcggt tggcgctgca cagcgccggc ccgcggttac tggcgagctt gccggcgact
     1141 tccatgagcg gcgcgacgac gtgcttgagc tgcatcacct gccgcttcag atcgtagagc
     1201 cgctcgatgt tggcgcgcgc cgtgccgcgg tcgaagatgc ggtcctcgat gctctccagt
     1261 tccgattcga ggcggtcgac aaccggaaaa tagcggtcga cgacggcgtc catcagcgcg
     1321 tagaggacaa aacccgagcc gtgctgcaga agatgcggtt cgcgctcgca acgcgcgcgc
     1381 acgccgagga aatcctgctg gctgcggttg cgcaccgaaa gcacgtaatt ggggccgacg
     1441 aagacattga cctcgccgag ccggtagccc tcgtccacat ccggctcgat caggtgcatg
     1501 acggcgaata tcgaatcgcc gtagtcctcg accttcggcc gctggtggcc cttgcgcgcg
     1561 tcctcgacgg ccagttcgtg caggtggaac tcctcctgca tgatgtccag ctcctccgtc
     1621 gtcgcatcgc gcaaggcgac ccagacgaaa caaccgggcc gttccaggta atcgctgatt
     1681 tcctcgacgg cgatctcgtt gagccgggtc ccgttctcgt aggccacgca attgatcagc
     1741 acggtcgcat cctgttcctg tctcctgaaa ggcagaagcc cccttgcggg ggcttgccgg
     1801 ggcaagcgcg gctggcgctc acatccgctc gatcatcgcg tcgccgaact ccgagcagga
     1861 aatctcgttg gcgccgacca tcaggcggg
//
LOCUS       DAOSDX010000628         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20943_length_1884_cov_4.058502, whole genome shotgun sequence.
ACCESSION   DAOSDX010000628 DAOSDX010000000
VERSION     DAOSDX010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20943_length_1884_cov_4.058502"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLN02_12400"
     CDS             <1..589
                     /locus_tag="PLN02_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOI53172.1"
                     /translation="GLQKELAADLLIAPPVADWVKAEPTLSDEEILARILEGAAQAYQ
                     AKVELVGTETFRQFERNVMLQSLDSYWREHLAALDHLRQGIHLRGYAQKNPKQEYKRE
                     AFELFETLLDQVRTQVTRVVFTVQIRSPEDVEETAPHADVSNVKYQHAGYDEALADEG
                     GDANQPVENGPKVGRNDPCPCGSGKKYKHCHGKLS"
     gene            652..1878
                     /gene="argJ"
                     /locus_tag="PLN02_12405"
     CDS             652..1878
                     /gene="argJ"
                     /locus_tag="PLN02_12405"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOI53173.1"
                     /translation="MPVNYATPSADQLFAVAGVRLGVAEAEIRKKNRRDLTLVALDAG
                     CTVAGVFTQNRFCAAPVQLCRQHLASGKEIRALVINTGIANAGTGEPGRQTARQTCEA
                     VGELLGVAAEQVLPFSTGVILEPLPVERIKAGLPAVFADLKADNWHAAAHGIMTTDTV
                     AKAASRVVEVNGRNVTISGISKGAGMIRPNMATMLGFLATDAGIAQPLLDQLVKEAAD
                     ASFNCITVDGDTSTNDSYVMIATGQSGASFAAASDAGWAEVKAAIVAVSVELAQAIVR
                     DGEGATKFITVAVEGGKTIEECRQVGYAIGHSPLVKTAFFASDPNLGRILAAVGYAGI
                     ADLDVDAVKVWLDDVLVAENGGRAAAYQEADGARVMAQAEITVRVALGRGDAAAKVYT
                     CDFSYDYVKINADYRS"
BASE COUNT          344 a          670 c          590 g          280 t
ORIGIN      
        1 cgggctgcag aaggaacttg ccgccgatct gctgatcgca ccgccggtcg ccgactgggt
       61 caaggccgag ccgacgctga gcgacgagga aatcctcgcc cgcatccttg aaggcgcggc
      121 ccaggcctac caggccaagg tcgaactggt cggtaccgag accttccgcc agttcgagcg
      181 caacgtcatg ctgcagagcc tcgattctta ctggcgcgag catctggcgg cgctcgacca
      241 cctgcgccag ggcatccacc tgcgcggcta cgcacagaag aacccgaagc aggaatacaa
      301 gcgcgaagcc ttcgaactgt tcgagaccct gctcgaccag gtgcggaccc aggtcacccg
      361 cgtcgtgttc acggtgcaga tccgctcgcc ggaagacgtc gaggaaaccg cgccgcacgc
      421 cgacgtcagc aacgtcaagt accagcatgc cggttacgac gaggcgctcg ccgacgaggg
      481 cggcgacgcc aaccagccgg tcgagaacgg accgaaggtc gggcgcaacg acccctgccc
      541 ctgcggttct ggcaagaagt acaagcactg tcacggcaag ctgtcctgaa attcgagtaa
      601 cccgatccaa ggcacccttc gcggtgcctt gatgctgttg gagtccttac catgcccgtc
      661 aattacgcca ccccgtccgc cgaccaactg ttcgccgtgg ccggcgtccg cctcggcgtc
      721 gccgaagccg aaatccgcaa gaagaaccgc cgcgacctga cgctggtcgc ccttgacgcc
      781 ggttgcacgg tcgctggcgt gttcacccag aaccgcttct gcgcggcgcc ggtccagctc
      841 tgccgccagc atctggcgag cggcaaggaa atccgcgctc tggtgatcaa caccggcatc
      901 gccaacgccg gcaccggcga gcccggccgc cagaccgccc gccagacctg cgaagcggtc
      961 ggcgaactgc tcggcgtggc ggcggaacag gtgctgccgt tctccaccgg cgtgatcctc
     1021 gagccgctgc cggtcgaacg catcaaggcc ggcctgccgg cggtcttcgc cgacctcaag
     1081 gcggacaact ggcacgccgc cgcgcacggc atcatgacta ccgacaccgt ggccaaggcg
     1141 gcttcgcgcg tggtcgaggt caatggcagg aatgtcacga tttccggcat ttccaagggc
     1201 gccggcatga tccggccgaa catggcgacc atgctcggct tcctcgccac cgacgccggc
     1261 atcgcccagc cgctgctcga ccagctggtc aaggaagcgg ccgacgcttc gttcaactgc
     1321 atcaccgtcg atggcgacac ctcgaccaac gattcctacg tgatgatcgc caccggccag
     1381 tcgggcgcca gcttcgccgc agcaagcgac gccggctggg ccgaggtcaa ggcggcgatc
     1441 gttgccgtgt cggtcgagct ggcgcaggcc atcgtccgcg acggcgaagg cgcgaccaag
     1501 ttcatcacgg ttgccgtcga gggcggcaaa acgatcgagg aatgccgcca ggtcggctac
     1561 gcgatcggcc actcgccgct ggtcaagacc gccttcttcg cttccgatcc caacctcggt
     1621 cgcatcctcg ccgccgtagg ttacgccggt atcgccgacc tcgacgtcga tgccgtcaag
     1681 gtctggctgg acgacgtgct ggtcgccgag aacggcggcc gtgccgccgc ctaccaggaa
     1741 gccgacggcg cccgggtgat ggcgcaggcc gagatcaccg tgcgtgtcgc cctcggtcgc
     1801 ggcgacgcgg cggccaaggt ctacacctgc gacttctcat acgactacgt caagatcaac
     1861 gccgactacc gctcgtaagc gatg
//
LOCUS       DAOSDX010000629         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20960_length_1883_cov_3.393326, whole genome shotgun sequence.
ACCESSION   DAOSDX010000629 DAOSDX010000000
VERSION     DAOSDX010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20960_length_1883_cov_3.393326"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..610)
                     /locus_tag="PLN02_12410"
     CDS             complement(2..610)
                     /locus_tag="PLN02_12410"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOI53174.1"
                     /translation="MRTKSEAKRQGILDAASEVFRVTGFEGASMSDICAKAGYSRATL
                     YSYFPSKEALFLEVMLSGSHAQAEVITRALDPETPSIEEALVHFGKDLLAFIYSPEIL
                     ALRRLAIAEATRSDFGEMFYARSRKQGEVIISAFLKQAMGAGKLRQADPSIAMAHLSG
                     LLESELEEMFYHTEIKPLDPLRLDRIARSAIGVFMAAYGPRS"
     gene            767..>1883
                     /locus_tag="PLN02_12415"
     CDS             767..>1883
                     /locus_tag="PLN02_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOI53175.1"
                     /translation="MAIAAALSGCAALPSGQTLQSIKAVDQYQSASSLSAPVVAWPDE
                     QWWQRYGDPQLDRLIQEGLANSPDMAVAAARLHAAEASRQVSNAALYPQISANGSVTE
                     QKQSYNYLTPRSMTPDGWQDYGRATIDFSWEIDFWGKNRAALAAATSELQASQAELAQ
                     ARLTLASSIASDYAELAHLFATRDTLAEALKVRTKTAELFAQRFANGLETRGSLREAE
                     ARQASAEGDLLQVDEQIALQRNRLAALLGAGPDRGLEIARPQFEFAHHFALPAELAVN
                     LLGRRPDIVAARLQVEASAKRIDEKKAGFYPNINLAAFIGVQSLGLDLLSKGGSSIGS
                     VGPAISLPIFNAGRLQGELRGARARHEEAVANYNKTLT"
BASE COUNT          381 a          597 c          554 g          351 t
ORIGIN      
        1 tctaggatct agggccgtaa gccgccatga agacaccgat agcgcttctg gcgattcggt
       61 cgagacgcag tggatccagg ggctttatct cggtgtggta gaacatttcc tccagttccg
      121 attccagcaa gccactcagg tgggccatgg caatcgaagg atcggcctga cgtagttttc
      181 cggcgcccat cgcctgtttc agaaaagcgg aaatgatgac ttcaccttgt ttgcggctac
      241 gcgcatagaa catttccccg aaatccgagc gcgtcgcctc ggcgatcgcc aggcgacgca
      301 aagccagaat ttccggtgag tagatgaaag ccagcaagtc cttgccaaaa tggacaagcg
      361 cttcctcgat actgggtgtt tccggatcca atgcccgggt gatcacttcg gcctgggcat
      421 gacttccgga aagcatgact tccagaaaca gggcttcctt cgaagggaaa tagctgtaca
      481 gcgtcgcccg ggagtaaccg gccttggcgc aaatatccga catcgacgcg ccttcgaaac
      541 cggtcacccg gaatacctcg gaagcggcat caaggatgcc ctggcgcttt gcctcgctct
      601 tggttctcat gacgactcct taactagact tgttcgttca gtatagcttg acttggtggc
      661 aagtcataac tagactggac cgtacagttt agttatgagg tgattcatgt cttctcaagc
      721 aattacgccg ccgcgccgcg ccttttcatc aagcacatgg atcgccatgg ccattgcggc
      781 agcgttgtcc ggttgtgccg cactcccttc tgggcagacg ctccagtcaa tcaaagcggt
      841 cgatcagtac cagagtgcgt cttccttgtc ggcgcccgtg gtcgcctggc ccgatgagca
      901 atggtggcaa cgctatggcg atccgcaact ggacaggctg atccaggaag gcctcgccaa
      961 ctcgccggac atggcggttg ccgccgcccg cctgcacgct gccgaagcca gccggcaggt
     1021 ttccaacgcg gccctctatc cgcagatttc ggccaacggc tcggtcaccg aacagaagca
     1081 gagctacaac tacctgacgc cgcgcagcat gacgccggat ggctggcagg actatggccg
     1141 ggcgacgatc gatttcagtt gggaaatcga cttctggggc aaaaatcgcg ctgcgcttgc
     1201 cgcagcgacc tcggaactgc aggccagcca ggcggaactg gcccaggccc ggctgacgct
     1261 ggcgagcagc atcgccagcg actacgccga actggcccac ctttttgcga cgcgcgacac
     1321 gctggccgag gcgctcaagg tacgcaccaa aacggcagag ctgttcgccc agcgttttgc
     1381 caacggtctg gaaacgcgcg gcagcctgcg cgaagccgag gccaggcagg cctccgccga
     1441 gggcgatttg ctgcaggtcg acgagcagat cgctttgcag cggaaccgtc tcgctgcctt
     1501 gctcggggcc ggaccggacc gcggtctcga gatcgcgcgg ccgcaattcg aattcgctca
     1561 ccacttcgcc ctgccggccg aactcgcggt caatctgctc ggccggcggc ccgacatcgt
     1621 cgccgcgcgc ctgcaggtcg aagccagcgc caagcgcatc gacgaaaaga aggccggttt
     1681 ctatccgaac atcaacctgg cggccttcat cggcgtgcaa tccctggggc tcgacctgct
     1741 cagcaagggc ggctcaagca tcggcagcgt cggcccggcg atttccctgc ccattttcaa
     1801 cgccggtcgt ctgcaaggcg aactgcgcgg cgcccgggca cgccatgaag aagcagtggc
     1861 caactacaac aagacgctga cgc
//
LOCUS       DAOSDX010000630         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_20965_length_1883_cov_2.464442, whole genome shotgun sequence.
ACCESSION   DAOSDX010000630 DAOSDX010000000
VERSION     DAOSDX010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20965_length_1883_cov_2.464442"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..>1883
                     /locus_tag="PLN02_12420"
     CDS             155..>1883
                     /locus_tag="PLN02_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOI53176.1"
                     /translation="MALTILGLSGALTHDPSAALYIDGKLVAAAEEERFVRDKHAKNR
                     MPYEAARFCLDFAGIKPKDVTAVAIPFAPISLADKARWHYAKRYWYAPDRALDALLTG
                     NRRFKRYKKRIEWCLAQLGLDLAKTEIIPVEHHLTHASSAYHCSGFQEKTAILGIDGK
                     GEYATTFFGVGENGKIRKIKEFYDPDSLGGLYGAITEYLGFEMLDGEYKVMGMAPYGD
                     PSKYDFSRLAKFENGELIINTDYANVIGFRRYKEKGKGYYFSPKLIEWLGPMRHGDIA
                     DDPYIHYAASMQKLFEDLSLQMMDYYLGDILKETGRLAFAGGGALNVKLNQKIIARPE
                     LKELFVQPASGDAGTAIGAAAYVSVERGVPVEKMEHVYLGPQFSNEDVIAACARHPAK
                     PQYEVIADGDTAVPERIAQILADGNPVAWFQGRMEFGPRALGGRSILGCPSAPGVADR
                     INEQIKFRERWRPFCPSMLDTVGPQMLGSDHPAPFMTFTFEVAEEWKSRVPEVVHEDG
                     TSRAQVLKREHNPRYYDLMKQMEKLTGNGVVLNTSLNRRGEPMICSPTDALNMFYGSD
                     LQYLIMQDVL"
BASE COUNT          373 a          678 c          541 g          291 t
ORIGIN      
        1 gccatcagaa actctgggac gccgacgact gggccgcgct gctcgccgcg caccgcgcgg
       61 cacgcggcac ctaagccgcg gcgaccgggt atactgcgag ctttccgccg gcagcccctt
      121 gttccggcag gtttttcaag aagagacacc cactttggca ctgaccatcc tcggcctttc
      181 cggcgccctc acccacgatc cttccgccgc cctctacatc gacggcaagc tcgtcgctgc
      241 cgccgaagag gagcgcttcg tccgcgacaa gcatgccaag aaccgcatgc cctacgaagc
      301 cgcccgtttc tgcctggact tcgccggcat caaaccgaag gacgtcaccg ccgtggccat
      361 tcccttcgcg ccgatcagcc tcgccgacaa ggcgcgctgg cactacgcca agcgctactg
      421 gtacgccccc gaccgcgcgc tcgacgcgct gctcaccggc aaccgccgct tcaagcgcta
      481 caagaagcgg atcgaatggt gcctggcgca gctcggtctc gacctcgcga agaccgagat
      541 catcccggtc gagcaccacc tgacgcacgc ctcctccgcc tatcactgct ccggcttcca
      601 ggagaaaaca gcgatcctcg gcatcgacgg caagggcgaa tacgcgacca ccttcttcgg
      661 cgtcggcgag aacggcaaga ttcgcaagat caaggagttc tacgacccgg actcgctcgg
      721 cggcttgtac ggcgcgatca ccgaatacct cggcttcgag atgctcgacg gcgaatacaa
      781 ggtgatgggc atggcgccct acggcgaccc aagcaagtac gatttctcgc gtctggccaa
      841 gttcgagaac ggcgagctga tcatcaacac cgactacgcc aacgtgatcg gcttccgccg
      901 ctacaaggaa aagggcaagg gctactactt ctcgcccaag ctgatcgaat ggctgggccc
      961 gatgcgccat ggcgacatcg ccgacgaccc ctacatccac tacgcggcga gcatgcagaa
     1021 gctgttcgag gatctgtcgc tgcagatgat ggactactac ctcggcgaca tcctcaagga
     1081 aaccggccgc ctcgcctttg ccggcggcgg cgcgctcaac gtcaagctca accagaagat
     1141 catcgcccgc ccggaactga aggaactctt cgtccagcca gcttccggtg acgccggcac
     1201 ggccattggc gccgccgcct acgtctcggt cgagcgcggc gtgccggtcg agaagatgga
     1261 acacgtctac ctcggcccgc agttcagcaa cgaagacgtc atcgccgcct gcgcccgcca
     1321 tccggccaag ccgcaatacg aagtcatcgc cgacggcgat accgccgtgc ccgaacgcat
     1381 cgcgcagatt ctcgccgacg gcaacccggt cgcctggttc caggggcgca tggaattcgg
     1441 cccgcgcgca ttgggcgggc gctccatcct cggctgcccg agcgcgcccg gcgtcgccga
     1501 ccggatcaac gaacagatca agttccgcga acgctggcgc cccttctgcc cgagcatgct
     1561 cgacacggtc ggcccgcaga tgctgggcag cgaccacccg gcgccgttca tgaccttcac
     1621 cttcgaagtc gccgaggaat ggaagagccg cgtgccggaa gtcgtccacg aagacggcac
     1681 ctcgcgcgct caggtcttga agcgcgagca caacccgcgc tactacgacc tgatgaagca
     1741 gatggaaaag ctcaccggca acggcgtcgt gctcaacacc tcgctcaacc ggcgcggcga
     1801 accgatgatc tgctcgccga ccgacgcgct gaacatgttc tacggttccg acctgcagta
     1861 cctgatcatg caggacgtgc tgg
//
LOCUS       DAOSDX010000631         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21003_length_1880_cov_2.425205, whole genome shotgun sequence.
ACCESSION   DAOSDX010000631 DAOSDX010000000
VERSION     DAOSDX010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21003_length_1880_cov_2.425205"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..738
                     /locus_tag="PLN02_12425"
     CDS             199..738
                     /locus_tag="PLN02_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOI53177.1"
                     /translation="MGGEAVFRVVRRRAKAGCAEAYEALVGAMFADARHFPGYVSAEL
                     IPPAEPGGDYQIIQRFAQQADLERWQASPVRAQWMERLAAVAEGEPEYRMLHGLDAWF
                     GPTLVPAQRMPRRWKMTLLSWCGIFPTVAFLLAFVAPLLAGLPFLLRTAVVTALVAVI
                     MSYLVMPRLTRLAADWLRR"
     gene            complement(749..1186)
                     /locus_tag="PLN02_12430"
     CDS             complement(749..1186)
                     /locus_tag="PLN02_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HOI53178.1"
                     /translation="MPVANLHALAGHPRENLKQWIRETSNALSEILAAPKDRLEVWVT
                     EIDPELWGINGLPADEVLAQTPRHDAEMPFIRMVLMEGRTTEQLHRVIAELTAITARL
                     LDVDAQRIRVYIAQAHPDRWGIGGVPASIRRAAELAARQPATG"
     gene            complement(1202..>1880)
                     /locus_tag="PLN02_12435"
     CDS             complement(1202..>1880)
                     /locus_tag="PLN02_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI53179.1"
                     /translation="DAGLGVIAVARSAASLRALTVQNPGVRACAADIGNDSAIAAIAA
                     AIDKPVRMVVHGPGVAVAGGILTAPTESMVDAVNIKVGGLLRLVRAADPQLAAGSRIV
                     AIGGHYGLEPTAYAAAAGVANAALLNVVRQLSMAYGQRGITAHTIVPGPADTERLRRV
                     AASRAEQRGCSTDDVLDEMRAESSLGILSTPEQIAWAVTLLLAPEADAMTGSSLMLDA
                     GRRRGLP"
BASE COUNT          265 a          570 c          694 g          351 t
ORIGIN      
        1 agcactgcct tcacacggca ggggtcactg gttcgaaccc agtaccgccc accatcggaa
       61 aaatacttga aacccgctcg gcgcaaggct cggcgggttt tttgttggat tttttccggg
      121 cgcatccgga aaaactgctg gccattcatt gcatgcactc tataaaatct cgatattaat
      181 tattttgtcg agattgttat gggcggcgaa gcggtgttcc gtgtggttcg acggcgggcc
      241 aaggcgggat gcgccgaagc ttacgaggcg ctagtcggcg cgatgtttgc cgatgcgcgg
      301 cattttccgg gttacgtgtc ggccgaattg attccgccgg cggagccggg gggcgattac
      361 cagatcatcc aacgctttgc gcagcaggcc gatctggagc gctggcaggc gtcgccggtg
      421 cgcgcgcaat ggatggaacg gctggcggcg gtggccgagg gcgagccgga gtaccggatg
      481 ctgcacggcc tcgatgcctg gttcggaccg acgctggtgc cggcgcagcg gatgccgcgg
      541 cgctggaaga tgaccttgct cagctggtgc ggcattttcc cgacggtggc gttcttgctc
      601 gcctttgtcg cgccattgct cgccggcctg cctttcctgt tgcgcacggc ggtggtgacg
      661 gcgctggtcg ccgtcatcat gtcctacctc gtcatgccgc gtctgacccg tctggcggcc
      721 gactggctgc gtcgctgagg cgacgggttc agccggtggc cggctgacgg gcggcaagtt
      781 cggcagcccg gcggatgctg gccgggacgc cgccgatgcc ccagcggtcg ggatgggctt
      841 gggcgatgta gacgcggatg cgctgcgcat cgacgtcgag caggcgggcg gtgatggcgg
      901 tcagttcggc gatcacccgg tgcagttgct cggtcgtccg gccttccatc aggaccatgc
      961 ggatgaaggg catctcggcg tcgtggcgcg gcgtttgcgc cagcacttcg tctgccggca
     1021 ggccgttgat gccccagagt tcgggatcga tttcggtgac ccagacttcc agtcgatctt
     1081 tcggggcggc gaggatttcc gacagtgcgt tcgaggtttc gcggatccac tgcttgaggt
     1141 tttcgcgcgg atggccggcg agggcgtgca ggttggcgac gggcatgttt ttctccgttg
     1201 atcaaggtag accgcggcgg cggccggcgt cgagcatcag gctggaaccg gtcatcgcat
     1261 cggcttccgg ggcgagcagc agggtcaccg cccaggcgat ctgttccggt gtgctgagga
     1321 tgccgagcga agattcggcg cgcatttcgt cgagcacgtc gtcggtgctg cagccgcgct
     1381 gttcggcgcg gctggcggcg acccggcgca ggcgctcggt gtcggccggg ccggggacga
     1441 tggtgtgggc ggtgatgccg cgctgtccgt aggccatgct caactggcgc acgacgttga
     1501 gcagagcggc gttggcgacg ccggcggcgg ccgcgtaggc agtcggctcc aggccgtagt
     1561 ggccgccgat ggcgacgatg cgcgaaccgg ccgccagttg cggatcggcc gcccgcacca
     1621 ggcgcaaaag cccgccgacc ttgatgttga ccgcatcgac catcgattcg gtcggcgccg
     1681 tcaggatgcc gccggccacc gcgacccccg gaccatgcac gaccatgcgc accggcttgt
     1741 cgatggcggc agcgatggcg gcgatcgccg agtcgttgcc gatgtcggcg gcacaggcac
     1801 gcacgccggg attttgcacg gtcagcgccc gcaggctggc ggccgagcgg gcgacggcga
     1861 tgacgccgag gccggcgtcg
//
LOCUS       DAOSDX010000632         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21084_length_1874_cov_3.402969, whole genome shotgun sequence.
ACCESSION   DAOSDX010000632 DAOSDX010000000
VERSION     DAOSDX010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21084_length_1874_cov_3.402969"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..>1874
                     /locus_tag="PLN02_12440"
     CDS             316..>1874
                     /locus_tag="PLN02_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOI53180.1"
                     /translation="MGKPDLPTAITTTLDDKYSRHTGRVFLTGTQALIRLPMLQRERD
                     LAAGLNTAGFVSGYRGSPLGNLDLGLWQAKAHLADHHVTFQPGVNEDLAATAVWGSQQ
                     VGLFPGAKYDGVFAMWYGKGPGVDRCGDVFRHANAAGSAKFGGVLVIAGDDHAAKSST
                     LPHQTEHVFKAVMMPVLYPANVQEYLDFGLHGWAMSRYSGCWVAFKALADTVETSASV
                     NIDAAAVDIRIPTDFSLPPDGLNIRWPDPPLVQEARLLNYKLYAALAYCRANRLDRVV
                     IDSPAPRLGILTAGKSYLDVRQALDELGIDDALAAQIGIRLYKVGMVWPLEPEGVRHF
                     ADGLEEILVVEEKRQLLEYQLKEELYNWRDDVRPRIVGKFDEKGEWTVEGEGGYLAHS
                     DWLLPAAGELPVASIARVIARRIGRFFTSPTIEARLKLIEGKQQALKTPLVLAERKPH
                     FCSGCPHNTSTKVPDGSRAVAGIGCHYMVTWMDRATSTFTHMGGEGVPWVGQAPFTEE
                     KHIFANLGDGT"
BASE COUNT          363 a          656 c          555 g          300 t
ORIGIN      
        1 cattcttgca acttgcctgc gcttacatct ggctaatgtg aacacgctct agggtcgcct
       61 tcttctttgg ctactttctt cttggcgaaa caagaagaaa gtacgcccgc cggtcaacgg
      121 cggaaaacag cggcaaataa accaaacaca cccgcccgtc aggcgcgaaa cacagcggct
      181 cagaaaacca agcccccttc ttgcaagaac catccctccc cccgatactc caatctccaa
      241 atcccgcccg ccaccacccc cggcgcgcag ggagacgaca accggctcac aaggccgata
      301 acaatggaga caaacatggg caagcccgat ctaccaacgg cgatcaccac gacgctggat
      361 gacaagtatt cccgccacac cggccgcgtc ttcctgaccg gcacccaggc gctgatccgc
      421 ctgcccatgc tgcaacggga gcgcgacctc gccgccgggc tgaataccgc cggtttcgtc
      481 tccggttacc gcggcagtcc gctcggcaac ctcgaccttg gcttgtggca agccaaggcc
      541 catctcgccg accaccacgt caccttccag cccggcgtca atgaagacct cgccgctacc
      601 gccgtctggg gcagccagca ggtcggactc ttccccggcg ccaaatacga cggcgtcttc
      661 gccatgtggt acggcaaggg gccgggcgtg gaccgctgcg gcgacgtctt ccgccatgcc
      721 aacgccgccg gttcggccaa gtttggcggc gttctggtca tcgccggcga cgaccacgcg
      781 gccaagtcct cgacgctgcc gcaccagacc gagcatgtct tcaaggcggt gatgatgccg
      841 gtgctctacc cggccaacgt ccaggagtac ctcgatttcg gcctgcacgg ctgggcgatg
      901 agccgctatt ccggctgctg ggtggcgttc aaggcgctgg ccgacacggt cgaaacctcg
      961 gcctcggtga atatcgacgc cgcagcggtc gacatcagga tcccgaccga tttcagcctg
     1021 ccgcccgacg gtctcaacat ccgctggccg gacccgccgc tggtccagga agcacgcctg
     1081 ctcaactaca agctgtacgc ggcgctcgcc tattgccggg cgaaccggct cgaccgcgtg
     1141 gtgatcgact cgccagcgcc gcgcctgggc atcctgacgg cggggaaaag ctatctcgat
     1201 gtgcgccagg cgctcgacga actgggcatc gacgacgcgc tcgccgcgca gatcggcatc
     1261 cgtctgtaca aagtcggcat ggtctggccg ctggagccgg aaggcgtgcg ccactttgcc
     1321 gatgggctgg aggaaatcct cgtcgtcgaa gagaaacgcc agttgctcga ataccagttg
     1381 aaggaagagc tgtacaactg gcgcgacgat gtccgtccgc gcatcgtcgg caagttcgac
     1441 gaaaagggcg aatggaccgt cgagggcgaa ggcggctatc tggcgcacag cgactggctg
     1501 ctgccggccg ccggcgagct accggtggcg agcattgccc gcgtcatcgc ccggcgcatc
     1561 gggcgcttct tcacctcgcc gaccatcgaa gcgcggctca aactgatcga aggcaagcag
     1621 caggcgttga agacaccgtt ggtgctggcc gagcgcaagc cgcacttctg ctccggctgc
     1681 ccgcacaaca cgtccaccaa agtcccggac ggctcgcgcg ccgtcgccgg catcggctgc
     1741 cattacatgg tgacctggat ggaccgcgcg acttcgacct tcacccacat gggcggcgag
     1801 ggcgtgccct gggtcggtca ggcaccgttc accgaagaga agcacatctt cgccaacctc
     1861 ggcgacggca ccta
//
LOCUS       DAOSDX010000633         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21086_length_1874_cov_3.249588, whole genome shotgun sequence.
ACCESSION   DAOSDX010000633 DAOSDX010000000
VERSION     DAOSDX010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21086_length_1874_cov_3.249588"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLN02_12445"
     CDS             complement(<1..967)
                     /locus_tag="PLN02_12445"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOI53181.1"
                     /translation="MLFGRTTLSKFVIEHTRGQHSDMGALLIDVAAAVKGISALVGKG
                     ALSGFSGSAATTNVQGEEQKKIDVLANEAILRHCEWGGQLAGMASEEMDEPYTIPDAY
                     PRGRYLLVFDPLDGSSNSDVNVSVGTIFSILQRPQAGAATPGDFLRPGAEQVAAGYAI
                     YGPATMLVLSVGTGTHAFTLDRESGNFILTHPDIRVPEATREFAINTSNERFWEPPVQ
                     RYVAECKAGKTGVRGADFNMRWIASMVAEVHRILMRGGIFMYPKDSKDPSKPGRLRLL
                     YEANPMAMLIEQAGGAATTGRGRILDVAPEALHQRVPVILGSREEV"
     gene            1146..1853
                     /locus_tag="PLN02_12450"
     CDS             1146..1853
                     /locus_tag="PLN02_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOI53182.1"
                     /translation="MQTAPALIFDVDGTLAETERDGHLPAFNAAFAEFGLPWVWDARL
                     YGELLEVTGGKERIRHFAGRYDPRRAAAADFGELVQQLHRAKTAHYVRSVAAGAIPLR
                     PGVAELIGAARAAGWRLAIATTTAPENVHALLRASLAPDAAGWFEVIGAGDVVPAKKP
                     APDIYDWVRERLGLPAAACLAIEDSANGLDAARAAGLRCLVCRGEYSRDQDFSAAWRV
                     VDSLEGIDPTSLFPSAP"
BASE COUNT          292 a          606 c          638 g          338 t
ORIGIN      
        1 cgacttcctc gcgcgagccg aggatcaccg gcacgcgctg gtgcagcgct tccggcgcga
       61 cgtcgaggat gcgcccgcgg ccggtggtgg cggcgccgcc ggcctgctcg atcagcatcg
      121 ccatcgggtt ggcttcgtag agcaggcgca ggcgcccggg cttcgacgga tccttgctgt
      181 ccttgggata catgaagatg ccgccgcgca tcaggatgcg atgcacttcg gcaaccatcg
      241 aggcgatcca gcgcatgttg aaatcggccc cgcgcacgcc ggtcttgccc gccttgcact
      301 cggcgacgta gcgctgtacc ggcggctccc agaaacgctc gttcgaggta ttgatggcga
      361 actcgcgggt ggcctccggc acgcggatat cgggatgggt gaggatgaag ttgccgctct
      421 cgcggtccag cgtgaaggcg tgggtaccgg tgccgacgct cagcaccagc atggtcgccg
      481 ggccgtagat ggcgtagccg gcggccacct gttcggcgcc cgggcgcagg aaatcgcccg
      541 gcgtcgcggc gccggcctgc ggccgctgca agatcgagaa gatggtaccg accgagacgt
      601 tcacgtcgct gttcgacgag ccgtcgagcg ggtcgaagac cagcaggtag cggccgcgcg
      661 ggtaggcgtc ggggatagtg tagggttcgt ccatctcctc cgacgccatg ccggcgagct
      721 ggccgcccca ttcgcagtgg cgcaggatgg cctcgttggc caggacgtcg atcttcttct
      781 gctcttcgcc ctgcacgttg gtcgttgccg ccgatccgga aaagccggac agcgcgccct
      841 tgccgaccag cgccgagatg cccttgaccg ccgctgcaac gtcgatcagc agggcgccca
      901 tgtcgctgtg ctgaccgcgg gtgtgttcga tgacgaactt cgacagcgtg gtacgtccga
      961 atagcatgat gtctccagtc attggtttgt taccgtgttg gagttcaaga taagttactt
     1021 gtgaattaag gcctagtcag tattttttaa tttatccata aatcattgct gattaaacaa
     1081 gtctgagccc tgggttcagg attcctgact ggcgatcgcg cggccgccgc actatcttgg
     1141 cgagcatgca aactgcaccc gccctgatct tcgatgtcga tggcacgctc gccgaaaccg
     1201 agcgtgacgg ccacctgccg gcgttcaacg ccgcctttgc cgaattcggt ctaccctggg
     1261 tctgggacgc ccgcctttat ggcgagctgc tggaagtcac cggcggcaag gaacggatcc
     1321 ggcatttcgc cggacgttac gatccgcggc gggcggccgc ggcggatttc ggggaactgg
     1381 tgcagcaact gcaccgggcg aagaccgcgc actatgtgcg cagcgtcgcc gccggcgcca
     1441 ttccgttgcg ccccggcgtc gccgagctga tcggtgcggc gcgggccgcc ggctggcggc
     1501 tggcgatcgc gacgacgacc gcgccggaga atgtacacgc gctgttgcgt gccagtctgg
     1561 cgccggacgc tgccggctgg ttcgaggtga tcggcgccgg cgacgtcgtg ccggccaaga
     1621 agccggcgcc cgacatctac gactgggtgc gcgagcgcct gggcctgccg gccgccgcct
     1681 gtctcgccat cgaggattcg gccaacggcc tggacgcggc gcgcgccgcc ggcctgcgct
     1741 gcctggtctg ccgtggcgaa tactcgcgcg accaggactt cagcgctgcc tggcgggtgg
     1801 tcgatagcct ggaaggcatc gacccgacga gcctctttcc ttctgcacca tgaaaaacgc
     1861 cgggaaatcc cggc
//
LOCUS       DAOSDX010000634         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21108_length_1873_cov_2.333883, whole genome shotgun sequence.
ACCESSION   DAOSDX010000634 DAOSDX010000000
VERSION     DAOSDX010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21108_length_1873_cov_2.333883"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /gene="lpxK"
                     /locus_tag="PLN02_12455"
     CDS             complement(<1..408)
                     /gene="lpxK"
                     /locus_tag="PLN02_12455"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOI53183.1"
                     /translation="MLAGWLQRHWYERRLTPAFWLLLPLLLPLNLLFAGVAGWRRRQA
                     RPQRLPVPVVVVGNLTVGGAGKTPLTLWLAGQLAARGFRPGIVSRGYGAVAAQPRPVL
                     AASTPAEVGDEPLLLARRSGVPVWVGRDRAAAGR"
     gene            complement(408..824)
                     /locus_tag="PLN02_12460"
     CDS             complement(408..824)
                     /locus_tag="PLN02_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOI53184.1"
                     /translation="MNFQRGRGREEPEINLIPMIDVLLVIIIFLMLTTTYSKFSGLEI
                     NLPTADVSQENQQPNEIDVAVTAAGQILVNKAPLASGEIEAISAALQQAAAGRKDPVI
                     VINADAKATHQRVIDVMQAAQSAGYPHISFATQSPE"
     gene            complement(828..1433)
                     /locus_tag="PLN02_12465"
     CDS             complement(828..1433)
                     /locus_tag="PLN02_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOI53185.1"
                     /translation="MFEIVKAAGWPIWPLLLASVISMALIIERLISLRRGKIVPAGLL
                     QRAVGEFRRGADNGKLLDELDRHSPLGRVLAAGLRNVNNSREVMKESIEEAGSAVAHE
                     LNRYLTTLGTIASISPLMGLFGTVIGMIEIFGSQAPGTGNPQQLAHGISIALYNTGFG
                     IFIAIPSLIFWRHFRALVDGFVVEMEQQAVRLVEVMHGDRK"
     gene            1719..>1873
                     /locus_tag="PLN02_12470"
     CDS             1719..>1873
                     /locus_tag="PLN02_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOI53186.1"
                     /translation="MPSPLPPVNNSVLSVSALNRLVRDCLESAFPLLWVGGEISNLTR
                     AASGHVY"
BASE COUNT          342 a          619 c          601 g          311 t
ORIGIN      
        1 ccggccggcc gccgcgcggt cgcgcccgac ccacaccggt acgccgctgc gccgggcaag
       61 cagcaagggt tcgtcgccga cttcggccgg cgtcgacgcc gccagcaccg gccgcggctg
      121 ggccgccaca gcgccgtagc cgcggctgac gatgccggga cgaaagccgc gcgccgccag
      181 ttggccggcc agccacaggg tcagcggcgt cttgccggcg ccgccgacgg tgaggttgcc
      241 gacgacgacg accggcaccg gcagccgctg cggccgcgcc tgccggcgcc gccagccggc
      301 aacgccggcg aacagcaggt tgagcggcag gagcagcggc agcagaagcc agaacgccgg
      361 cgtcagccgg cgttcgtacc agtgtcgctg caaccagccg gcgagcatca ctcgggcgac
      421 tgggtggcga aggagatgtg cgggtagccc gccgactgcg cggcctgcat gacgtcgatg
      481 acgcgctggt gggtggcttt ggcgtcggcg ttgatgacga tcaccggatc cttgcgcccg
      541 gccgccgcct gctgcagcgc cgccgaaatc gcctcgatct cgccgctggc gagcggcgcc
      601 ttgttgacca ggatctgacc ggcggcggtg accgcgacgt cgatctcgtt cggctgctgg
      661 ttttcctggc tgacgtcggc cgtcggcagg ttgatctcga ggccggagaa cttcgagtaa
      721 gtggtcgtca gcatcaggaa gatgatgatc accagcaaca cgtcgatcat cgggatcagg
      781 ttgatttccg gctcttcgcg gccgcgtccg cgctggaaat tcatcgctta cttccggtcg
      841 ccgtgcatca cttcgaccag gcgcaccgcc tgctgctcca tctcgacgac gaagccatcg
      901 accagcgcgc ggaaatggcg ccagaagatc aggctgggaa tcgcgatgaa gatgccgaag
      961 ccggtgttgt acagggcgat cgaaatgccg tgcgccagtt gctgcgggtt gccggtgccc
     1021 ggcgcctgcg agccgaagat ctcgatcatc ccgatgacgg tgccgaacag ccccatcagc
     1081 gggctgatcg aggcgatggt gccgagcgtc gtcaggtagc ggttgagttc gtgggcgacc
     1141 gccgaaccgg cctcctcgat cgattccttc atcacttccc gggaattgtt cacgttgcgc
     1201 aggccggcag ccagcacgcg gccgagcggc gagtggcggt ccagttcatc gagcagcttg
     1261 ccattgtcgg cgccacgccg gaactcgccg accgcccgct gcaacaggcc cgccggcacg
     1321 atctttccac gacgcaggct gatcaggcgc tcgatgatca gcgccatgga gatgaccgag
     1381 gccagcaaca gaggccagat gggccagccg gccgctttga cgatttcaaa cacttgacga
     1441 tgtccttggc gaaagtttag tccgcaattc tgcctccccg gcggcgcagg ggcaagcgcg
     1501 acgattttcc ggggccgccg cggcttatcc ccaaaagctg tggataagtc tgtggaagag
     1561 ctgtcatcag ttgccgcaaa cccgcaagaa acaaggcttt cgcctaccct gccacaaaac
     1621 gaggcataga ataaaaacag cacaaaatca ataacttgaa aatcaccaga aatgtcaagc
     1681 gcctaccatg aaaagtctga ccacggtaga atgacgccat gccttctcct ctgccgcctg
     1741 tcaataactc cgtactcagc gtcagcgcgc tcaaccgtct ggtgcgcgat tgtctggagt
     1801 ccgcctttcc gctgctctgg gtgggcggtg aaatttccaa cctgacccgc gccgcctccg
     1861 gccacgtcta ttt
//
LOCUS       DAOSDX010000635         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21119_length_1872_cov_2.713814, whole genome shotgun sequence.
ACCESSION   DAOSDX010000635 DAOSDX010000000
VERSION     DAOSDX010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21119_length_1872_cov_2.713814"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1872)
                     /locus_tag="PLN02_12475"
     CDS             complement(<1..>1872)
                     /locus_tag="PLN02_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI53187.1"
                     /translation="DEAQRLGKLLIEKSGRDFTTAERLALDIVAKNIGATLSRIRQAS
                     AGKQLESRLRQLSRALDSSLNGVMITSSTQLDHPIVYVNPAFEKITGYSASEVIGRSG
                     RFLIGHDLQQRGLAAIREALRKRHQAHAILRNYRKDGSLFWNELFIAPVRDESGSFTT
                     HFVSIINDVSERIGYEKQLEFHANHDPLTGLANRNLLNDRIAQAIVRARSDGMMTGLL
                     QLGLDRFRWINDSLGHASANEVLKEVAQRLRECVRDTDTVARLAGDEFVIVIGRLTTT
                     NELAGIANKILRLVNQPIPCADRKIVVTASIGAAIYPGDGEQGDTLLRHADIAMHQAK
                     AEERNSYRFFMPTTRASVIDRLSLEGDLRHAIAHDELVVHYQPLVELSGGTIIGAEAL
                     VRWQHPQRGLIPPDEFIPLAEESGLIIDIGEQVLRQVCRDLGRWRDSGLQAGRISINL
                     SARQFRRDGLAASIHRSLTESGIGGRQLAFEVTESVVMHDLEHTAQLLTEIRELGASI
                     SLDDFGTGYSSLAYLRRLPIDILKIDRSFVRDLHLEPDAAAIAHAVIAMAHKLGLGVV
                     AEGVECAAQLALLREFDCEEGQGFLFSRPLPPEQYAIILKQGRLPLASPPADAPRRPS
                     "
BASE COUNT          339 a          518 c          622 g          393 t
ORIGIN      
        1 gctcgggcgg cggggggcgt cggctggtgg ggaggccagt ggcagccgtc cttgtttcag
       61 gatgatggcg tattgttcgg gcggcaacgg gcggctgaac agaaagccct ggccttcttc
      121 gcagtcgaat tcccgcagca gggccagttg cgcagcgcat tcgacacctt ccgcgacgac
      181 gccaagcccg agtttgtgcg ccatggcgat gacggcatga gcgatggctg cggcgtcggg
      241 ttcgaggtgc aggtcacgaa cgaagctgcg atcgatcttc aagatgtcga ttggcaggcg
      301 gcgcaggtag gcgagactgg agtagccggt gccgaagtcg tcgagcgaaa tgctggcacc
      361 cagctcgcga atttcggtca gtagctgcgc agtgtgctcg agatcgtgca tgaccacgct
      421 ttcggtcact tcgaaggcca actgtcgtcc gccaatgcct gattcggtga gactccggtg
      481 gatgctggca gcgaggccgt cgcgccggaa ctggcgggcc gagagattga tcgatatccg
      541 gccagcttgc aagccactgt cgcgccatcg gccgaggtcg cggcaaacct gacgcaagac
      601 ctgttcgccg atgtcgatga tgaggccgct ttcttccgcc agcgggatga attcgtcggg
      661 agggatcaag ccgcgttgcg gatgttgcca gcgcaccagc gcttcggcgc cgatgatcgt
      721 gccgccgctc agttcaacca ggggttgata atgcaccacc aactcatcgt gcgcgatggc
      781 gtgacgcaaa tcgccttcca ggctgaggcg gtcgatgacg gaggctcgag tggtcggcat
      841 gaaaaagcga tagctgttgc gctcctccgc ctttgcctga tgcatcgcga tatcggcgtg
      901 gcgcagcagg gtatcccctt gctctccgtc gccggggtag atggcggcgc cgatcgacgc
      961 agtgacgacg attttccgat cggcgcaagg gatgggctga ttgacaagac gcagtatctt
     1021 gttggcgatg ccggccaatt cgttcgtcgt cgtcaatctg ccgatgacga tgacgaattc
     1081 gtcaccggcg agccgggcta cggtgtcggt atcgcgaacg cattcgcgca ggcgctgtgc
     1141 cacctctttc agtacttcgt ttgccgaagc gtggccgaga ctgtcgttga tccagcgaaa
     1201 gcggtccaga ccgagttgca gcaaaccagt catcatgccg tcgcttcgtg cccggacgat
     1261 cgcctgcgcg attcggtcgt tgagcaggtt acggtttgcc agaccggtca gggggtcatg
     1321 gttggcgtgg aattccagtt gcttctcgta accgatgcgt tcgctgacgt cattgatgat
     1381 gctgacgaaa tgcgtggtga aactaccgct ttcgtcgcgc accggggcga tgaatagttc
     1441 gttccagaac agggagccgt ccttgcgata attacgcaag atggcatggg cctggtggcg
     1501 tttgcgcagt gcctcgcgaa tcgccgccag accgcgttgc tgcaggtcat gaccgatcag
     1561 gaagcgaccg ctgcgaccga tgacttcaga ggcggagtag ccagtgatct tctcgaatgc
     1621 cgggttgacg tagacgatag gatggtcgag ctgggtcgag gacgtgatca tcacaccgtt
     1681 gaggctcgaa tcaagcgccc gggaaagctg gcgcaagcgg gattccagtt gttttccggc
     1741 gcttgcttga cggatccggg acagcgtggc gccgatgttt ttggctacga tgtcgagcgc
     1801 gagacgttct gcggtcgtaa aatcccgtcc gctcttttca atgagcagtt ttcccagccg
     1861 ctgtgcttca tc
//
LOCUS       DAOSDX010000636         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21155_length_1869_cov_2.783903, whole genome shotgun sequence.
ACCESSION   DAOSDX010000636 DAOSDX010000000
VERSION     DAOSDX010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21155_length_1869_cov_2.783903"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLN02_12480"
     CDS             complement(<1..317)
                     /locus_tag="PLN02_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOI53188.1"
                     /translation="MSRAEMDRLGWDSCDIILITGDAYIDHPSFGMALIGRLLEAQGF
                     RVGIISQPDWNSAADFRQLGKPNLFFGVTAGNMDSMINRYTADRKPRSDDAYTPHAVA
                     DKRP"
     gene            487..837
                     /locus_tag="PLN02_12485"
     CDS             487..837
                     /locus_tag="PLN02_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI53189.1"
                     /translation="MMQLTTREQSGVLVVSVSGRIDHASSEEFTRLLEPLLINCRQGQ
                     PALLLDFSGVAYISSAGLRVLMMASRQSKAQQGSFAIAALTPLVQEVFTISRFNLIVP
                     CYTSVDTACKVLGQ"
     gene            834..1796
                     /locus_tag="PLN02_12490"
     CDS             834..1796
                     /locus_tag="PLN02_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI53190.1"
                     /translation="MKQVIFWGTRGSLPTAPTGQVIREKIIAALAAANGRSFRNRDAL
                     EQFVGGLPFTVNNTFGGNSSCVEIVTDGPDHLICDMGSGARPLGQSKIARYGVPNPQT
                     YHIFISHLHWDHLMGFPFFAPMYIPGNHIVIHGCHQQLEEAVRMQMRAPCFPVDYSQA
                     GARIDFDLMTPDQPHEIGGVRVTPKLQLHTGDSYGYRFDSTDKSAVYSTDSEHKLDDL
                     VSFDCFARFFAKADLVIFDAMYSLAEATSVKADWGHSSNVVGVELCQAAAAKRLALFH
                     HEPAHDDAQLFRLFEESIRFEQITRGDRPPLDIVSAYDGLTINL"
BASE COUNT          343 a          624 c          553 g          349 t
ORIGIN      
        1 gggcgcttgt cggcgacggc atgcggcgta taggcatcat ccgagcgcgg cttgcggtcg
       61 gcggtgtagc ggttaatcat cgaatccatg ttgccggcgg tgacgccgaa aaacagattc
      121 ggtttgccca gctggcgaaa atcggccgcc gaattccagt ccggctggct gatgatgccg
      181 acccggaaac cctgggcttc gaggagccgc ccgatcagcg ccatgccgaa gctcgggtga
      241 tcgatgtagg catcgccggt gatcaggatg atgtcgcagg aatcccagcc gagccggtcc
      301 atttcggcgc gcgacatggg gaggaagggg gcgggaccaa aacgctgggc ccagaatttg
      361 cgccaggaaa acaggggacg cggcgcggtg gaaacgttgg tattgagcat gcaatatttt
      421 acctaagtca gaggacggcc atggcatact tgaccattga aacaattgcc tgaagctttt
      481 ccagcgatga tgcaactaac cacccgcgag cagtccggcg ttctggtcgt ctccgtcagc
      541 ggtcgcatag accacgcgtc cagtgaagag ttcacccgtt tgctcgaacc actgctgatc
      601 aactgccgcc agggtcagcc cgcattgctg cttgattttt ccggcgtcgc ctatatcagc
      661 agtgccggcc tgcgcgtgct gatgatggcc tcccgccagt cgaaagcgca gcagggcagc
      721 ttcgccatcg ccgccctgac gccgctggtc caggaagtgt tcacgatcag ccgtttcaac
      781 ctcatcgtcc cctgttacac ctcggtggac accgcctgca aggtgctcgg acaatgaagc
      841 aggtcatttt ctggggtacc cgcggctcgc tgccgacggc gccgaccggg caggtcatcc
      901 gcgagaagat catcgccgca ctggctgccg ccaacggacg cagcttccgg aaccgcgatg
      961 cgctcgaaca gttcgtcggc ggcctgccgt tcaccgtgaa caacactttc ggtggcaatt
     1021 cgtcgtgcgt cgagatcgtc accgacggcc ctgaccacct gatctgcgac atgggaagcg
     1081 gcgcccggcc gctcggccag agcaagatcg cccgctacgg cgtgcccaac ccgcagacct
     1141 accacatttt catctcgcat ctgcactggg accacctgat gggtttcccg ttctttgcgc
     1201 cgatgtacat ccccggcaac cacatcgtca tccacggttg ccaccagcag cttgaagagg
     1261 cagtgcgcat gcagatgcgg gcgccctgct tcccggtcga ctattcgcag gctggcgcca
     1321 ggatcgattt cgacctgatg acgccggacc agccgcacga gatcggcggc gtcagggtca
     1381 cgcccaagct gcagctacat accggcgact cttacggcta ccgtttcgac agcaccgaca
     1441 aatcggcggt ctatagcacc gactccgagc acaagctcga cgacctcgtc tccttcgact
     1501 gtttcgcccg ctttttcgcc aaggccgacc tggtcatttt cgacgccatg tattcgctcg
     1561 ccgaggccac ttcggtcaag gccgactggg ggcattcgag caatgtcgtc ggcgtcgagc
     1621 tgtgccaggc cgctgccgcc aaacgcctgg ccctcttcca ccacgaaccg gcgcatgacg
     1681 acgcccagct cttccgcctg ttcgaggaat cgatccgctt cgagcagatc acccgcggcg
     1741 accggccgcc gctggacatc gtttccgcct acgacggcct gaccatcaac ttgtgagccg
     1801 ggccaccgcc gtcatcgccg ccctgctgcg cgccgggcgc ggacgcttgc tgcccagcct
     1861 gctcctcgg
//
LOCUS       DAOSDX010000637         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21160_length_1869_cov_1.951488, whole genome shotgun sequence.
ACCESSION   DAOSDX010000637 DAOSDX010000000
VERSION     DAOSDX010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21160_length_1869_cov_1.951488"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLN02_12495"
     CDS             complement(<1..296)
                     /locus_tag="PLN02_12495"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HOI53191.1"
                     /translation="MLTVMPAAADECPERRNWSLALRVDNDLFGGRGQDQGYSNGLQL
                     TAVSPNIADYAGDPCLSAPARALNRYLDWLQPGAYEQRNMVVAVQQALYTPADK"
     gene            489..1676
                     /gene="dapC"
                     /locus_tag="PLN02_12500"
     CDS             489..1676
                     /gene="dapC"
                     /locus_tag="PLN02_12500"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004365193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HOI53192.1"
                     /translation="MNPHLAQLQPYPFEKLRALFAGFTPNPQYREIKLSIGEPQHATP
                     QFIMDALHVNLRGLANYPTTQGIPALRQAIAAWCARRYAISVDPESEVLPVNGSREAL
                     FSFAQAVIDPSRGRPIVVSPNPFYQIYEGAAYLAGAEPRFLNNLPENGFAFDYEQLTP
                     EEWARVQLFYVCSPGNPTGKVLSLADWKTLFALSDKYGFVIASDECYSEIYFDEAKPP
                     IGGLQAAKLLGRNFDRLVMFSSLSKRSNVPGMRSGFVAGDAKLLKAFLLYRTYHGCAM
                     APPVQAASVAAWNDEAHVLDNRDQYREKFAACTPLIASALGTAMPDASFYLWAKTPIA
                     DSEFARGLLEHYNVVVLPGSFLARDSQGVNPGAGFVRIALVAGLEECLEASRRIVQFT
                     QQL"
     gene            1695..>1869
                     /locus_tag="PLN02_12505"
     CDS             1695..>1869
                     /locus_tag="PLN02_12505"
                     /inference="COORDINATES: protein motif:HMM:NF026155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOI53193.1"
                     /translation="MTHPLQATIDELWEKRTELTTQSTDAIKIIESVIGDLDSGKLRV
                     AEKVNGEWVVNQWA"
BASE COUNT          362 a          649 c          542 g          316 t
ORIGIN      
        1 aacttgtcgg ccggggtgta caaggcctgc tgcacggcga ccaccatgtt gcgctgctcg
       61 taggcgcccg gctgcagcca gtcgagatag cggttgagcg cgcgggccgg cgccgacaga
      121 caaggatcgc cggcatagtc ggcaatattc ggcgacaccg cggtcaattg cagaccgttg
      181 ctgtagccct ggtcctggcc gcgcccaccg aacaggtcgt tatcgacgcg cagcgccagc
      241 gaccagttgc ggcgttccgg gcattcgtcg gcggccgctg gcatgaccgt cagcaacgcc
      301 ggaagcagca gcagaaaacg cagcggtgga ggaatcgaca acggcatcgg aagatccagc
      361 gaaggaacgc cccgatgata accgcccttt tgcactgcct gccagcggcc gccgggcaac
      421 gcagcggaac gaccccggct cgtccggttt gggcgataat cgcgcctttc gacagctcct
      481 gtacgccagt gaatccccac ctcgcccaac ttcagcccta ccccttcgaa aagctgcgcg
      541 cgctgttcgc cggtttcacc cccaacccgc aataccggga aatcaagctg tcgatcggcg
      601 aaccgcagca cgcgacgccg cagttcatca tggacgcgct acacgtcaac ctgcgcggcc
      661 tcgccaacta cccgacgact cagggcatcc cggcgctgcg ccaggcgatc gccgcctggt
      721 gcgcccgccg ctacgccatc tccgtcgatc cggaaagcga agtcctgccg gtcaatggtt
      781 cgcgcgaagc gctgttctcc ttcgcccagg cggtgatcga cccgagccgg ggccgcccga
      841 tcgtcgtcag ccccaatccc ttttaccaga tctatgaagg cgcggcctac ctggccgggg
      901 ccgagccgcg ctttctgaac aacctgccgg aaaacggctt cgccttcgac tacgagcaat
      961 tgacgccgga agaatgggcg cgcgtgcaac tattctacgt ctgttcgccg ggcaacccga
     1021 ccggcaaggt attgtcgctc gccgactgga agacgctgtt cgcgctgtcc gacaagtacg
     1081 gtttcgtcat cgcctccgac gaatgctatt cggaaattta tttcgacgag gccaagccgc
     1141 cgatcggcgg cctccaggcg gcgaagctgc tcggccgcaa tttcgaccgg ctggtgatgt
     1201 tctccagcct gtcgaaacgt tccaacgtgc ccggcatgcg ttccggcttc gtcgccggcg
     1261 atgcgaagct gctcaaggct ttcctgctct accgcaccta ccacggctgc gccatggcac
     1321 ctccagtgca ggccgcctcg gtcgccgcct ggaacgacga ggcgcacgtc ctggacaacc
     1381 gcgaccagta ccgcgaaaaa ttcgccgcct gcacgccgct gatcgcctcg gcgctcggca
     1441 ccgccatgcc ggacgcaagt ttttacctgt gggcaaaaac gccgatcgcc gatagcgaat
     1501 tcgcccgcgg cctgctcgaa cactataatg tcgtagtcct gcccggcagt ttccttgccc
     1561 gcgattccca gggcgtcaac ccgggtgccg gtttcgtccg catcgccttg gtcgccggcc
     1621 tggaagaatg cctggaagcc agccgccgca tcgttcaatt cacacaacaa ctgtaaccaa
     1681 gagagacccg ccaaatgact catcccctgc aagccaccat cgacgaactc tgggaaaagc
     1741 gtaccgagct gaccacccaa tcgaccgacg cgatcaagat catcgaatcc gtgatcggcg
     1801 atctcgacag cggcaagctg cgtgtcgccg agaaggtcaa tggcgaatgg gtggtcaacc
     1861 agtgggcca
//
LOCUS       DAOSDX010000638         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21182_length_1867_cov_3.352097, whole genome shotgun sequence.
ACCESSION   DAOSDX010000638 DAOSDX010000000
VERSION     DAOSDX010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21182_length_1867_cov_3.352097"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1867
                     /gene="nuoG"
                     /locus_tag="PLN02_12510"
     CDS             <1..>1867
                     /gene="nuoG"
                     /locus_tag="PLN02_12510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOI53194.1"
                     /translation="MEFLLINHPLDCPICDQGGECQLQDMSVGYGPMQSRYTEEKHVV
                     FHKNVGPLISMEEMSRCIHCTRCVRFGQEIAGIMELGMANRNMHSEIQTFVGRSVDSE
                     LSGNMIDLCPVGALTSKPFRYTARSWELQRRKSVSPHDSVGANLVVQVKHDSVMRVLP
                     RENEAVNECWISDKERFSYQALNTDQRLTKPMVKQGGEWVECDWNVALDYVAHGLKDI
                     VRDHGGDAVAALATQNATVEELYLLGKLMKGLGSGNVDFRARQSDFTSDGKRAGAPWL
                     GLRLAEIKDLDAALVIGSFLRKDHPLIAQRLRQAAKKYTKVSLVSVTADDQLINLHGR
                     LTVAPSQLVFALAAVVRAAAEAKGMATPAGLENVAVCDESRKIAQSLLDGEKRALFLG
                     NVATQSVDATQIHALTCLLGEITGASVGFLGESANTVGAHLAGALPTAANARQMFEQP
                     RQGYVLFGVEPEFDCAHPQQALGALRQAQMVVVMSAFKHDPALDYADVMLPLAPYTET
                     SGTFVNIEGRIQSFNGVVKPLGAARPAWKVLRVLGNVLALDGFDYMSSEAVRDEVLGG
                     DKEFAAGLDNGLSGVAVALPAVASGGLQRIADVPIHFADAMARRAPALQQTADGKA"
BASE COUNT          327 a          618 c          593 g          329 t
ORIGIN      
        1 tgatggagtt tctgctcatc aaccatccgc tcgattgccc gatctgcgac cagggtggcg
       61 aatgccagct gcaggacatg tcggtcggct acggcccgat gcagagccgc tacactgaag
      121 agaagcacgt cgttttccac aagaacgtcg gtccgctgat ctcgatggaa gagatgagcc
      181 gctgcatcca ctgcacgcgc tgcgtccgct tcggccagga aatcgccggc atcatggaac
      241 tcggcatggc caaccgcaac atgcactccg agatccagac cttcgtcggc cgctcggtcg
      301 attccgagct ctcgggcaac atgatcgatc tgtgcccggt cggtgccctg acctcgaagc
      361 ctttccgcta caccgcgcgt tcctgggaac tgcagcggcg caagtcggtc agcccgcatg
      421 actcggtcgg tgccaatctg gtcgtccagg tcaagcacga cagcgtgatg cgcgtcttgc
      481 cgcgcgagaa cgaggcggtc aacgagtgct ggatttccga caaggagcgt ttctcctacc
      541 aggcactcaa taccgatcag cgcttgacca agccgatggt caagcagggt ggcgagtggg
      601 tcgaatgtga ctggaacgtc gcacttgact acgtcgccca cggtctcaag gacatcgtcc
      661 gcgaccacgg cggcgacgct gtcgccgcat tggcgaccca gaacgccacg gtcgaggaac
      721 tctacctgct gggcaagctg atgaaggggc tgggcagcgg caacgtcgat ttccgcgccc
      781 gccagtccga cttcaccagc gatggcaagc gcgccggcgc gccgtggctt ggcctgcgtc
      841 tggccgaaat caaggatctc gacgcggcgc tggtgatcgg ttccttcctg cgcaaggatc
      901 acccgctgat cgcccaacgc ctgcgccagg ccgccaagaa atacaccaag gtcagcctcg
      961 tctcggtgac ggcggacgat cagctgatca acctgcacgg ccggctgacc gttgcgcctt
     1021 cgcaactggt cttcgcgctg gcggcggtgg tgcgtgctgc ggctgaagcc aagggaatgg
     1081 cgacgccggc cggtctggaa aatgtcgcgg tctgcgacga aagccgcaag atcgcgcaaa
     1141 gcctgctcga cggtgaaaaa cgcgcgctct tcctcggcaa cgttgccacg caatcggtcg
     1201 atgccaccca gatccatgcg ctgacctgcc tgctcggtga aatcaccggc gccagcgtcg
     1261 gtttccttgg cgagtccgcg aataccgtcg gtgcccacct cgctggcgcc ctgccgaccg
     1321 ctgccaacgc ccgtcagatg ttcgagcagc cgcgtcaggg ctatgtcctg ttcggcgtcg
     1381 agccggaatt cgactgcgcc cacccgcagc aggccctggg cgcgctgcgc caggcgcaaa
     1441 tggtcgtcgt catgtcggcg ttcaagcatg atccggcgct cgattacgcc gacgtgatgc
     1501 tgccgctcgc cccttacacc gaaacctccg gcaccttcgt caatatcgag gggcgcatcc
     1561 agtccttcaa tggcgtcgtc aagccgcttg gcgcggcgcg tccggcctgg aaggtgctgc
     1621 gcgtcctcgg caacgtgctg gcgctcgacg gtttcgacta catgagcagc gaagcggtgc
     1681 gtgacgaggt gttgggtggt gacaaggagt tcgccgccgg cctcgacaac gggctgagcg
     1741 gtgtcgccgt ggccttgccg gccgtcgcca gtggcggctt gcagcgcatc gccgatgtgc
     1801 cgatccactt tgccgatgcc atggcgcgcc gcgcgccggc cctgcagcag acggccgacg
     1861 gcaaggc
//
LOCUS       DAOSDX010000639         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21222_length_1864_cov_4.487562, whole genome shotgun sequence.
ACCESSION   DAOSDX010000639 DAOSDX010000000
VERSION     DAOSDX010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21222_length_1864_cov_4.487562"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1082)
                     /locus_tag="PLN02_12515"
     CDS             complement(171..1082)
                     /locus_tag="PLN02_12515"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOI53195.1"
                     /translation="MSPSPSATGEAIEDSQRRALRQRAEYERRLAAGENPAPLVARID
                     LEELAGSERYRRVSELEQVDQNSETCLRGQPLVFHTPSRRLLWQALGQEYIEPELLDY
                     IDDMEGGSVFYDIGASNGIFALYAAVTGKQVIGFEPEIENFALLNRNTYLNRQRIAAG
                     MSSFNIALSSQTTLGRLFIKRFEAGGHLKILDRPVERGFEAFEPEYVQAVMKYRLDDF
                     IRVSGIPRPNHLKIDVDGCELEVIQGMSETLADAGLRSIFIELEENNEQSEACRRIIE
                     GAGFQLAKKKRVQNYFGENNCVFRRHG"
     gene            complement(1079..>1864)
                     /locus_tag="PLN02_12520"
     CDS             complement(1079..>1864)
                     /locus_tag="PLN02_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53196.1"
                     /translation="KLLVRLAPGAGYKIAVDDILDFVARHRQALAGSCVEVAGFPDPL
                     AFAGIDDLLAGLDALGPLTVSTYMPADGLLTPRTRHPLRLRLFALAREAEFRSLFPDT
                     AWPRAEREIAAFLERIPGNLPTQVVRTGFTGPAPRLPERANIIYADDLSYPWDYTALL
                     HYASHPTAAARHDLEQCCGFDVDAALAKARLQREKNCMCERVFPVFNADGSLATCHLY
                     SAALLNRTPLAVSLAEIIATRKFNPHCRTCQDAGLHRLDLRLL"
BASE COUNT          296 a          544 c          673 g          351 t
ORIGIN      
        1 ggatgcaggc gatcgcgctg gcgccctggg caacgaaggc gttcagggtg gccaggccga
       61 tgccgcggtt gcagccggtg atgatggcga gtttgtcttt caggagcatg aatcgttatg
      121 ccgtgatggg aggtccggga tgcctggcgc cgaagcggac ggcagggtgg ctagccatgg
      181 cgccggaaga cgcagttgtt ttcgccgaaa tagttctgca cccgcttctt ctttgccagc
      241 tggaagccgg cgccttcgat gatgcggcgg caagcttccg actgttcgtt attttcctcc
      301 agttcgatga aaatcgaacg cagtccggcg tcggccaggg tttcggacat tccctgaatg
      361 acctcgagtt cgcagccgtc cacgtcgatc ttcagatggt tggggcgggg aatgccgctg
      421 acgcggatga agtcgtcgag ccgatacttc atcaccgcct gcacgtattc gggctcgaat
      481 gcctcgaagc cccgctcgac cgggcggtcg agtatcttca ggtggccgcc ggcctcgaag
      541 cgcttgatga acagccggcc cagcgtcgtc tggctggaaa gggcgatgtt gaacgaactc
      601 atgccggcgg cgatgcgctg gcggttgagg taggtgttgc ggttgagcag cgcgaagttc
      661 tcgatttccg gttcgaagcc gatcacctgc ttgccggtca ccgcggcgta gagggcaaag
      721 atgccgttgc tggcgccgat gtcgtagaag accgagccgc cttccatgtc gtcgatgtag
      781 tcgagcagct cgggctcgat gtattcctgg ccgagggctt gccagagcag ccgcctggac
      841 ggggtgtgga acaccagcgg ctggccgcgc aagcaggtct cgctgttctg gtcgacctgc
      901 tcgagttcgc tgacgcgccg gtagcgttcg ctgccggcca gttcttcgag gtcgatccgt
      961 gccaccagcg gcgccggatt ttctccggcc gccagtcgcc tttcgtattc cgcgcgctgc
     1021 ctgagggcgc ggcgctggct gtcttcaatg gcttctccgg tggcggatgg tgaggggctc
     1081 atagcagtct caggtccaaa cgatgcaggc cggcatcctg acaggtgcgg cagtgcgggt
     1141 tgaatttccg ggtggcgatg atttctgcca gcgatacggc gagcggcgtc cggttcaaca
     1201 gcgccgcgct gtagaggtgg caggtggcga gcgaaccgtc tgcgttgaag accggaaaga
     1261 cccgttcgca catgcagttc ttctcgcgct gcagccgggc cttggcgagc gccgcatcga
     1321 cgtcgaagcc gcagcattgc tcgaggtcgt gccgggcggc ggccgtcggg tggctcgcgt
     1381 agtgcagcag cgccgtgtag tcccagggat aggacagatc gtcggcgtag atgatgtttg
     1441 cccgttcggg cagccggggc gcggggccgg taaagccggt cctgacgacc tgggtcggca
     1501 ggttgccggg aatccgctcg agaaaggcgg cgatttcccg ctccgccctc ggccaggcgg
     1561 tgtcgggaaa aaggctgcgg aattccgctt cccgcgccag tgcaaacagg cgcaggcgca
     1621 gcgggtggcg ggtgcggggc gtcagcagcc cgtccgccgg catgtaggtg ctgacggtga
     1681 gcgggcccaa ggcgtcgagt ccggccagca ggtcgtcgat gccggcaaag gccagcgggt
     1741 cgggaaaccc ggcgacttcg acgcaactgc cggcgagcgc ctggcggtgc ctggcgacga
     1801 aatcgagaat gtcgtcgacc gcgatcttgt agccggcgcc gggcgcgaga cggaccagca
     1861 gctt
//
LOCUS       DAOSDX010000640         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21239_length_1863_cov_2.497788, whole genome shotgun sequence.
ACCESSION   DAOSDX010000640 DAOSDX010000000
VERSION     DAOSDX010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21239_length_1863_cov_2.497788"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /gene="nadE"
                     /locus_tag="PLN02_12525"
     CDS             <1..1110
                     /gene="nadE"
                     /locus_tag="PLN02_12525"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOI53197.1"
                     /translation="LFAAASRRHKLPLVYVNQIGGHDQLVYDGASFAVTPEEGVVFEA
                     PRFVEAQVTLQWDGTAFRAGDGAPLAPVVAAGLPVMEFYRRQIVLGLRDYTRRCGFKQ
                     VVVGSSGGIDSALTLALAAEALGPENVVAVTLPSRFSSSGSVDDSVTLCENLGIKLYT
                     HPIKALVGQCEADFGAAFGEPLQGLALENLQARVRGTVLMAYSNRYGHLLLTTGNKSE
                     ISVGYCTLYGDTNGGLGLIGDLYKTEVFELCRHLNAEAGGELIPLAIIAKEPSAELAP
                     DQRDSDSLPPYPVLDTILKVLIEGERLGAAERAAVQADYATLAAGPDGPALIARVRRL
                     IARNEYKRRQAPPILRLRGRAFGSGRQMPIAAKYD"
     gene            1121..>1863
                     /locus_tag="PLN02_12530"
     CDS             1121..>1863
                     /locus_tag="PLN02_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOI53198.1"
                     /translation="MSKEKFDIAVVIGRFQPFHNGHAALLAQALGCADKVIVLLGSAH
                     AARSAKNPFSWEERASMIALTLDEATKARVTFLPLRDYYDERRWRAAAQAAVQAQAAP
                     GARIALIGHAKDATSEYLHGFPGWTLLPAPAYGAINATSLRRILFEGEDRAATQALLA
                     SQAPATVVHYLLGWLNLPFVAGLREEHARVEAGKKTWGTGPFITVDAVVTAAGHVLLI
                     ERGQPPGKGQWALPGGFLEPRERVLQGAMR"
BASE COUNT          290 a          671 c          637 g          265 t
ORIGIN      
        1 ttgttcgccg ccgccagccg ccgccacaag ctgccgctgg tctatgtgaa ccagatcggc
       61 ggccacgacc aactggtcta cgacggcgcc tccttcgcgg tgacgccgga ggagggcgtg
      121 gtcttcgagg cgccgcgttt cgtcgaagcg caagtcacgc tgcaatggga cggcaccgcc
      181 ttccgtgccg gcgacggcgc gccactggcg ccggtcgttg cggccgggct gccggtgatg
      241 gagttctacc gccggcagat cgtcctcggc ctgcgcgact acacccgccg ctgcggcttc
      301 aagcaggtgg tggtcggttc ctccggcggc atcgactcgg cgctgaccct ggcgctcgcc
      361 gccgaggcgc tggggccgga gaacgttgtc gcggtgacgc tgccctcgcg cttttccagc
      421 agcggcagcg tcgatgactc ggtgacgctg tgtgaaaacc tcggcatcaa gctgtacacg
      481 cacccgatca aggcgctggt cggccagtgc gaggccgatt tcggcgccgc cttcggcgag
      541 ccgctgcaag ggctggcgct ggaaaacctg caggcgcgcg tgcgcggcac cgtgctcatg
      601 gcctactcca accgctatgg ccacctgctg ctgaccaccg gcaacaagag cgagatttcg
      661 gtcggctact gcacgctcta cggcgacacc aacggcggcc tcggcctgat cggcgacctc
      721 tacaagaccg aggtcttcga gctgtgccgc cacctcaatg cggaagccgg cggcgagctg
      781 atcccgctgg cgatcatagc caaggagccg tcggccgagc tggcacccga ccagcgcgac
      841 agcgacagcc tgccgcctta cccggtgctc gacaccatcc tcaaggtgct gatcgagggc
      901 gagcgcctgg gtgcggccga acgtgctgcc gtgcaggccg actacgccac gctcgccgcc
      961 ggccccgacg gcccggcgct gatcgcccgc gtgcgtcggc tgatcgcccg caacgagtac
     1021 aagcgccgcc aggccccgcc catcctccgc ctgcgcggcc gcgccttcgg cagcgggcgg
     1081 cagatgccga ttgccgccaa atacgattga ggagccgagc atgagcaaag aaaaattcga
     1141 catcgccgtc gtcatcggcc gcttccagcc tttccacaac ggccacgcgg cgctgctggc
     1201 gcaggcgctg ggctgtgcgg acaaagtcat cgtcctgctc ggttcggcac acgccgcgcg
     1261 cagcgccaag aacccgttca gttgggaaga acgggcgagc atgatcgcgc taacgctgga
     1321 cgaggcgacg aaagcgcgcg tgaccttcct gccgctgcgc gactactacg acgaacgccg
     1381 ctggcgggcg gcggcgcagg ccgcggtgca ggcgcaggcg gcgccggggg cgcgcatcgc
     1441 gctgatcggc cacgccaagg acgcaaccag cgaatacctg cacggcttcc ccggctggac
     1501 gctgctgccg gcgccggcct acggcgcgat caacgccacc agcctgcgcc gcatcctgtt
     1561 cgagggcgag gaccgcgccg cgacgcaggc gctgctcgcc agccaggcgc cggccaccgt
     1621 cgtccattac ctgctgggct ggctgaacct gccgttcgtt gccggcctgc gcgaggaaca
     1681 cgcgcgcgtc gaagccggca agaaaacctg gggcaccggg ccgttcatca ccgtcgacgc
     1741 ggtggtcacc gccgccggcc acgtactgct gatcgaacgc ggccagccgc ccggcaaggg
     1801 ccagtgggcg ctgcccggcg gtttcctgga gccgcgcgag cgggtcttgc agggagcgat
     1861 gcg
//
LOCUS       DAOSDX010000641         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21261_length_1861_cov_3.822259, whole genome shotgun sequence.
ACCESSION   DAOSDX010000641 DAOSDX010000000
VERSION     DAOSDX010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21261_length_1861_cov_3.822259"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLN02_12535"
     CDS             complement(<1..392)
                     /locus_tag="PLN02_12535"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435434.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOI53199.1"
                     /translation="MSKAPMRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDL
                     PQAQKAVQGVMMELDDCAFPLLAGMVATDDPNVAFKDADVCLLVGARPRGPGMERADL
                     LQANGAIFTVQGKAIAENAKEDVRVLVVG"
     gene            590..1345
                     /locus_tag="PLN02_12540"
     CDS             590..1345
                     /locus_tag="PLN02_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOI53200.1"
                     /translation="MTRETLRSAARASAPTFSPLYRQIKDFLIGCLERGEWRPGDVIP
                     SEGELAQRFNVSQGTVRKAIDEMAAENLLVRRQGKGTFVSTHSDPRAYYRFLRLMPND
                     GIAVPSVSEPFYCQVVDAPPEVAAVFGLAPGGAVVHIKRLLRFGGEKVVFDQIFLQAE
                     FFPGLTLELLRAGERSLYSLLESDFGVRMISAEEHLRAVAADEECAALLGVQCGEPLL
                     LVERTAYTYGNRPVEWRKGLYCTRNHYYRNELG"
     gene            1467..>1861
                     /gene="sdhC"
                     /locus_tag="PLN02_12545"
     CDS             1467..>1861
                     /gene="sdhC"
                     /locus_tag="PLN02_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOI53201.1"
                     /translation="MAEMMIKKERPRNLDLASLRSYKLPLPGKVSIFHRVSGAGLFLM
                     LPVILWLFQASVTSPETFAVFQGLVASWPVKLILAGLIWAYAHHFCAGIRFLLMDVHV
                     GVDKPQARKSAAAVFAVSIPLSIILWGVLL"
BASE COUNT          322 a          529 c          604 g          406 t
ORIGIN      
        1 ccgacgacca gcacgcggac gtcttccttg gcgttctcgg cgatggcctt gccctgaacg
       61 gtgaagatcg cgccgttggc ttgcagcagg tcggcacgtt ccatgccggg gccgcgcgga
      121 cgggcgccga ccagcaggca gacgtcggcg tccttgaagg cgacgttcgg gtcgtcggtg
      181 gcgaccatgc cggcgagcag cgggaaggcg cagtcgtcga gttccatcat cacaccttgc
      241 acggccttct gggcttgcgg caggtcgagc agttgcagga tgaccggctg gtccttgccg
      301 agcatttcac ccgaggcaat gcggaacagc aggctataac cgatctgacc ggcggcgccg
      361 gtcacggcga cgcgcatggg ggctttggac atgtatcgct cctgaattta aagcagcttg
      421 gttgaattat tggtcgattc cgggagccga cgaattttcc ttgcgtcgga tcggctagcg
      481 gaattcgcga tagtagaagt tcgatctatc cgtgtcaatt gattcatgtg tcttatataa
      541 gacaactttt ggtggacgct ggcgccgaat tatgctgaaa tcgaggccca tgacccgaga
      601 gaccctgcgt tccgctgccc gcgcatccgc gccgaccttc agccccttgt accgccagat
      661 caaggatttc ctgatcggct gtctcgagcg cggcgagtgg cgcccgggtg atgtgatccc
      721 cagcgaaggc gaactggcgc agcgcttcaa tgtcagccag ggcacggtgc gcaaggccat
      781 cgacgagatg gcggcggaga acctgctggt gcggcggcag ggcaagggaa ccttcgtttc
      841 aacccatagc gacccacgtg cctactatcg tttcctgcgc ctgatgccga atgacggcat
      901 cgccgtgccc tcggtcagcg aacccttcta ttgccaggtg gtcgatgcgc cgcccgaggt
      961 ggcggcggtg ttcggtctgg cgccaggggg ggcggtggtg catatcaagc gtttgctgcg
     1021 ttttggcggc gagaaggtcg tcttcgacca gattttcctg caggcggagt tttttccggg
     1081 cttgacgcta gaactgttgc gtgccggcga gcgttcgctg tacagcctgc tggagagtga
     1141 tttcggcgta cgcatgatca gcgccgaaga acatttgcgt gccgtggctg ccgacgagga
     1201 atgcgcggcg ctgctcggcg tgcagtgcgg cgagccgctg ttgctggtcg agcgcaccgc
     1261 ctacacctac ggcaatcggc cggtggagtg gcgcaagggc ctgtactgca cgcgcaacca
     1321 ttattaccga aacgaactgg ggtgatcgct gaaactgctg caagggccgc caactggcgc
     1381 atcggggctc gtttgactgt ataattacgg ctcttttcaa accacataca agcggaaatc
     1441 cgcactcttc gcgagggaat atcttcatgg cagagatgat gataaagaag gagcgtccaa
     1501 gaaatctgga cctggcttcg ctgcgtagtt acaaattgcc gcttccgggc aaggtttcca
     1561 tcttccaccg ggtgagcggg gcggggctgt tcctgatgtt gccggtcatt ctctggctct
     1621 ttcaggccag cgtgacctcg ccggagactt ttgcggtctt tcaggggttg gtggcttcct
     1681 ggccggtcaa gctgattctg gcaggtctga tctgggccta tgcgcaccat ttctgcgccg
     1741 gcatccgttt cctgctgatg gacgtccatg tcggcgtcga taagccgcag gcacgcaagt
     1801 ccgccgctgc cgtgttcgcg gtcagcattc cgctgagcat cattctgtgg ggggttctgc
     1861 t
//
LOCUS       DAOSDX010000642         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21262_length_1861_cov_3.515504, whole genome shotgun sequence.
ACCESSION   DAOSDX010000642 DAOSDX010000000
VERSION     DAOSDX010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21262_length_1861_cov_3.515504"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PLN02_12550"
     CDS             <1..1071
                     /locus_tag="PLN02_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOI53202.1"
                     /translation="RLMTIAMAPFMSCGARLPVYVLFAAAFFPSNAQNVVFALYLTGI
                     AVAVLTGLMLKKTLLKGEATPFIMELPPYHLPTVKGILLHTWDRLQGFIVRAGRIIVP
                     MVLVLNFLNALGTDGSYGNEDSDKSVLAEIGRTISPAFSPIGLNADNWPAAVGIFTGV
                     LAKEAVVGTLDAAYTALAASDATATGDAPAEEAPFALGEAIAAAFATIPVNLADALGT
                     WADPLGIAVGDLSDTTAVAEENEVHTGTFGAMASRFDGAGGAFAYLLFILLYFPCTAA
                     IAAVHHESGTRWTLFVGAWTTGLAYGMATIAYQLSIFSRAPAVSAGWIAAVLAMAAAF
                     IWFLRQQGHKADRLAAAQPQEA"
     gene            1073..1306
                     /locus_tag="PLN02_12555"
     CDS             1073..1306
                     /locus_tag="PLN02_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HOI53203.1"
                     /translation="MATILSRVEQVLREQHRASLRDLAESLDTAPEALRGMLELLRRK
                     GRVRQLPGGTACGGGCSKCRPETVEIFEWIAPQ"
     gene            1303..1815
                     /locus_tag="PLN02_12560"
     CDS             1303..1815
                     /locus_tag="PLN02_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI53204.1"
                     /translation="MSCTASPDPLHDLYASHHGWLSGWLRRKLGCPEQAADLAQDTFL
                     RILGARDALPAICEPRAYLTTTANRLIIDRARRKALEQAYLAELSAVADTRHSFPSPE
                     ETLIAVQALMQIANALERVPVRAGEAFLLHYLDGMTHAEVACQLDVSPRMVRKYLIQC
                     LLACDLQSAA"
BASE COUNT          296 a          714 c          553 g          298 t
ORIGIN      
        1 cgcctgatga ccatcgccat ggcccccttc atgtcctgcg gcgcccgcct gccggtctat
       61 gtcctgttcg ccgccgcctt cttcccgagc aacgcgcaga acgtcgtttt cgccctctat
      121 ctgaccggca tcgcggtcgc cgtgctgacc ggactgatgc tcaaaaagac cctgctcaag
      181 ggcgaagcga cgcctttcat catggaactg ccgccctatc acctgccgac cgtcaagggc
      241 atcctgctgc acacctggga ccgcctccag ggcttcatcg tccgcgccgg ccgcatcatc
      301 gtgccgatgg tgctggtgct caacttcctc aacgcgctgg gcaccgacgg cagctacggc
      361 aacgaggaca gcgacaagtc ggtgctcgcc gaaatcggcc gcacgatctc gccggccttc
      421 tcgcccatcg gcctgaacgc cgacaactgg ccggccgccg tcggcatctt caccggcgtg
      481 ctggccaagg aagcggtcgt cggcaccctc gacgccgcct acaccgcact cgccgcaagc
      541 gatgcaacgg caaccggcga tgcccccgca gaggaagcgc cgttcgcgct tggcgaggcc
      601 atcgctgctg ccttcgccac catccccgtc aatctcgccg acgccctcgg cacctgggcc
      661 gatccgctcg gcatcgcggt cggcgacctc agcgacacga ccgccgtcgc cgaggaaaac
      721 gaagttcaca ccggcacctt cggcgccatg gccagccgct tcgacggcgc tggcggcgcc
      781 ttcgcctacc tgctcttcat cctgctctac ttcccctgca ccgccgccat tgccgcggtc
      841 caccacgaaa gcggcacgcg ctggaccctc ttcgtcggcg cctggaccac cggcctcgcc
      901 tacggcatgg cgaccatcgc ctaccagttg agcatcttca gccgcgctcc ggccgtctcg
      961 gcaggctgga tcgccgccgt cctggccatg gccgccgctt tcatctggtt cctgcgccag
     1021 cagggccaca aggcggaccg cctggccgcc gcccagccgc aggaggcctg agatggcgac
     1081 gattctgtcc cgggtcgaac aggtgttgcg cgagcaacac cgcgccagcc tgcgcgacct
     1141 ggccgaatcg ctcgacaccg caccggaagc gttgcgcggc atgctcgaac tgctgcgccg
     1201 caagggccgc gtccgtcagt tgccgggcgg caccgcctgt ggcggcggat gcagcaaatg
     1261 ccggccggaa acggtcgaaa tcttcgaatg gatcgccccg cagtgagctg caccgccagc
     1321 cccgacccgt tgcacgactt gtacgccagc catcatggct ggctgtccgg ctggctgcga
     1381 cgcaaactgg gctgcccgga gcaggctgcc gatctcgcgc aggacacctt cctgcgcatt
     1441 ctcggcgccc gcgacgcctt gccggcgatc tgcgagccgc gcgcctacct gaccacgacc
     1501 gccaaccggc tgatcatcga ccgggcgcgg agaaaagcgc ttgaacaggc ctacctagcc
     1561 gagctttcgg cggtggccga cacccggcac agctttccct cgcccgagga aaccctgatc
     1621 gcggttcagg ccctgatgca gattgccaac gccctcgagc gggtgccggt gcgggctggc
     1681 gaggctttcc tgctgcacta cctcgacggc atgacgcatg ccgaggtggc ctgccagctc
     1741 gacgtcagcc cacgcatggt ccgcaagtat ctgatccagt gcctgctcgc ctgcgacctt
     1801 cagtccgccg catgaaaccc tcaccctgca cgacggcaag cgaacaggcg gccgcgtgga
     1861 t
//
LOCUS       DAOSDX010000643         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21316_length_1857_cov_3.071587, whole genome shotgun sequence.
ACCESSION   DAOSDX010000643 DAOSDX010000000
VERSION     DAOSDX010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21316_length_1857_cov_3.071587"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="PLN02_12565"
     CDS             <1..1709
                     /locus_tag="PLN02_12565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI53205.1"
                     /translation="DITERKRLGQELDAHRHHLEQLVAERTKELEEARAQAVAANQSK
                     SEFLANMSHEIRTPMNAIIGLTHLLRQESTTTHHIDWLGKIDGAAKHLLAIINDILDL
                     SKIEAGKIVLEMRDFSVAGLLDEVSSLIGEQARAKALAVTMHLDGVPAWLRGDSTRLR
                     QGMLNYAGNALKFTTAGSIALRASLLDQRDGRYLVRFEVQDTGIGIAPEALPRLFQAF
                     EQADASTTRKFGGTGLGLAITRRFARLMGGDAGVESAPERGSTFWFTAWLDAGQPVTQ
                     TPQSAFGTSCEEELRRRHEGTRLLLAEDNPINREVALELLRSAGLVVDTAPDGVHALD
                     MARISDYALILMDMQMPEMDGLEATRAIRALPGRGRDELPILAMTANAFTEDRQACNE
                     AGMNDFVAKPVDPDALYATLLKWLPAVTPRTVPGERGGAGDTLAAGMDDEALYARLST
                     EPGMDVGEGIRMLLGKRDRYLSLLREMVDSHGSDPERVQTALLAGDRTTALRVAHTLK
                     GVAAQLGASGLADAAGDLENCLHDGERTAGSDLEILCDAVDAKLQRLSLLINSTVRSH
                     IS"
BASE COUNT          392 a          599 c          568 g          298 t
ORIGIN      
        1 atgacatcac cgagaggaaa cgccttggcc aggaactcga cgcccatcgc catcacctcg
       61 aacaactggt ggcagagcgc accaaggagc tggaagaggc acgtgcgcag gccgtggcag
      121 ccaaccagtc gaaatcggaa tttctcgcca acatgagcca tgagatacgg acgccgatga
      181 atgcgatcat cgggctgacc catctgttgc gacaagagtc tacgacgact caccatatcg
      241 actggctggg caagattgac ggtgcggcaa agcacttgct ggcgatcatc aacgacatac
      301 tcgacctgtc gaagatcgaa gcgggcaaaa ttgttcttga aatgcgggat ttttcggtcg
      361 ccggcctgct cgacgaggtc agctcgctga tcggtgagca agcccgggcc aaggcgctgg
      421 ccgtgacgat gcatctcgac ggcgtgcccg cctggctgcg cggtgattcc acccgcctgc
      481 gccagggcat gctcaactat gccggcaatg cgctgaaatt caccaccgca ggcagcatcg
      541 ccttgcgtgc cagcctgctg gatcaacgcg atggccgcta cctcgtacgt ttcgaggtac
      601 aggatacggg gatcggcatc gccccggagg ccctgccacg cttgttccag gctttcgaac
      661 aagccgacgc ctcgaccacg cgcaaattcg gcggaaccgg tctcggcctc gccatcaccc
      721 ggcgctttgc ccgtctgatg ggcggtgacg ccggtgtcga aagcgcgccg gaacggggca
      781 gcactttctg gttcaccgcc tggctcgatg ccggccaacc ggtgacgcag acaccccagt
      841 cggccttcgg cacgagttgt gaggaagaat tgcgccgccg ccatgaggga acccgactcc
      901 tgcttgcgga agacaatccg atcaaccgcg aggtagcgct ggaactgctg cgcagtgcgg
      961 gacttgtcgt ggataccgcc cccgacggcg ttcacgcact cgacatggcg cgtatcagtg
     1021 attacgcctt gatcctcatg gacatgcaga tgccggaaat ggacgggctg gaagcgacgc
     1081 gagccatccg cgccctaccg ggccgcggac gcgatgagct gccaattctg gccatgacag
     1141 caaatgcctt caccgaggac cgccaggctt gcaacgaagc gggcatgaac gatttcgtcg
     1201 ccaagccggt cgatccggat gcactctacg caaccctgct gaaatggttg ccggccgtca
     1261 cgccgcgaac cgttccgggc gaacgaggcg gcgctggcga caccctggcg gccggcatgg
     1321 atgacgaagc actgtacgcg cggctcagca cggaaccggg catggatgtc ggcgaaggca
     1381 tccggatgct gctcggcaag cgcgaccgtt acctgagcct gctgcgggaa atggtcgaca
     1441 gtcatggcag cgacccggaa cgcgtgcaga cggcgctgct cgccggagat cgcacaacgg
     1501 cgttgcgcgt cgcccacacg ctcaagggcg tggcagccca actcggcgcc agcggacttg
     1561 cagatgcagc cggggatctt gaaaactgcc tgcacgatgg cgaaaggacc gccgggagcg
     1621 acctggaaat cctctgtgac gcggtcgatg caaagctgca gcgactcagc ctgctgatca
     1681 acagcaccgt tcgcagccat atctcctaag agcgatcccg caacgaagca attgccgggt
     1741 acaaatcccg gtccaacact ccccctcctc gcccaggcaa tacgcctccc gctacccgcc
     1801 ggatcaagcg agctgaactg acattttcgg caaggaacgg atcgccaaaa gcgccct
//
LOCUS       DAOSDX010000644         1854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21361_length_1854_cov_1.922735, whole genome shotgun sequence.
ACCESSION   DAOSDX010000644 DAOSDX010000000
VERSION     DAOSDX010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21361_length_1854_cov_1.922735"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /gene="sdhA"
                     /locus_tag="PLN02_12570"
     CDS             complement(<1..1524)
                     /gene="sdhA"
                     /locus_tag="PLN02_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOI53206.1"
                     /translation="MSIPVLKFDAVIVGAGGAGLRSAIQLSEAGLKTAVLSKVFPTRS
                     HTVAAQGGVAASLGNSEEDHWHWHMYDTVKGSDWLGDQDAIEFMVRKANECVVELEHY
                     GMPFDRTDDGKIYQRPFGGHMSNFGEKPVRRSCAAADRTGHAMLHAMYQRNVKANTQF
                     FVEWMALDLIRDADGHVVGVTAMEMETGQVVIFHTRATIFATGGAGRIFYSSTNAFIN
                     TGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILRNANKERFMERY
                     APNAKDLASRDVVSRAMTTEINEGRGCGVNKDYVLLDITHLDPNTIMKRLPGIREIGL
                     QFAGVDCIKEPLPVVPTCHYQMGGIPTHYSGRVVVSENGDHNTVVPGFYAAGECACAS
                     VHGANRLGTNSLLDLLVFGKSAGDSAVEDLKTGQAHRDLPKDAAEKSLARISAIDNRK
                     GGANVHETRLAMQRTMQNHAGVFRFGDLLKSGVEKILEVEKAARALEIKDKSMAWNTA
                     "
     gene            complement(1527..>1854)
                     /gene="sdhD"
                     /locus_tag="PLN02_12575"
     CDS             complement(1527..>1854)
                     /gene="sdhD"
                     /locus_tag="PLN02_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HOI53207.1"
                     /translation="GAHYGLKDWVAQRATAVIMAVYSVILAAVLLVFRPSTFEAWQAV
                     FANGVIKFFTFLFFVSLFYHAWIGVRDIWMDYIKPVGLRLTLHVLTIAALVGYAAWAA
                     AILWRL"
BASE COUNT          367 a          554 c          604 g          329 t
ORIGIN      
        1 ggccgtgttc caggccatcg acttgtcctt gatctcgagc gcgcgggccg ccttctcgac
       61 ttccaggatc ttctcgacgc cggacttgag caggtcgccg aaacggaaca caccggcgtg
      121 gttctgcatc gtgcgttgca tggccagacg ggtttcgtgc acgttggcac cgcccttgcg
      181 gttgtcgatg gcggaaatcc gggccagcga cttctcggca gcgtccttcg gcagatcgcg
      241 gtgagcctgg ccggtcttga gatcttcgac cgcggagtca ccggccgact tgccgaagac
      301 cagcaggtcg agcagcgagt tggtaccgag gcggttggca ccgtgcaccg aggcgcaggc
      361 gcattcaccg gcggcataga agcccgggac gacggtgttg tgatcgccgt tctcgctgac
      421 cacgacacga cccgagtaat gcgtcgggat gccgcccatc tggtagtgac aggtcggcac
      481 gaccggcagc ggttccttga tgcaatcgac gccggcgaat tgcaggccga tttcacggat
      541 acccggcagg cgcttcatga tggtgttcgg gtcgaggtgg gtgatgtcga gcaggacgta
      601 gtccttgttg acgccacagc cgcggccttc gttgatttcg gtggtcatgg cgcgggaaac
      661 cacgtcacgc gacgccagat ccttggcgtt cggcgcgtag cgctccatga agcgttcctt
      721 gttggcgtta cgcaggatgc cgccttcgcc acggacgcct tcggtgatca gcacgccggc
      781 accggcgacg ccggtcgggt ggaattgcca gaattccatg tcttcgagcg ggatgccggc
      841 acgcgccgcc atacccaggc cgtcaccggt gttgatgaag gcgttggtcg aagagtagaa
      901 gatacggccg gcaccgccgg tcgcgaagat ggtggcgcgg gtgtggaaga tgacaacctg
      961 accggtctcc atttccatcg ccgtgacgcc gacgacatga ccatcagcat cgcggatcag
     1021 gtcgagggcc atccattcaa cgaagaactg ggtattggcc ttgacgttgc gctggtacat
     1081 agcgtgcagc atggcgtgac cagtacggtc agcagcggca caggaacggc gcaccggctt
     1141 ttcgccgaag ttcgacatgt ggccgccgaa cggacgctgg tagatcttgc cgtcgtcggt
     1201 acggtcgaac ggcatgccgt aatgttcaag ctcgaccacg cactcgttgg ccttgcgcac
     1261 catgaactcg atcgcgtcct ggtcgccgag ccagtcggaa cccttgacgg tgtcgtacat
     1321 gtgccagtgc cagtgatctt cctcggagtt gccgagcgag gcagcaacgc cgccctgagc
     1381 ggcaacggta tgcgaacggg tcgggaagac cttggacagg acggccgtct tcaggccggc
     1441 ttccgacaac tggattgcgg aacgcagacc ggcgccaccg gcaccgacaa tgaccgcatc
     1501 aaacttcaga acaggaatac tcacgcttac agcctccaga gaatcgcagc agcccacgcg
     1561 gcgtaaccca ccagcgcggc aatggtcagg acatgcaggg tcaggcgcag gccgaccggt
     1621 ttgatgtagt ccatccagat gtcacgcacg ccgatccagg cgtggtagaa caggctgacg
     1681 aagaacagga aggtaaagaa cttgatgacg ccattggcga aaaccgcctg ccaggcttca
     1741 aaggtggaag gacgaaacac cagcagcacg gcagccagga tgaccgaata gacagccatg
     1801 atgacggcag tggcgcgctg tgcgacccag tccttgaggc cgtaatgggc gccg
//
LOCUS       DAOSDX010000645         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21408_length_1850_cov_2.757103, whole genome shotgun sequence.
ACCESSION   DAOSDX010000645 DAOSDX010000000
VERSION     DAOSDX010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21408_length_1850_cov_2.757103"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLN02_12580"
     CDS             complement(<1..421)
                     /locus_tag="PLN02_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOI53208.1"
                     /translation="MLAKRIIPCLDVTAGRVVKGTNFVGLRDAGDPIEIARRYNEQGA
                     DEVTFLDITASSDRRDIILHIVEACAEQVFIPLTVGGGVRKVEDVRRLLNAGADKVSM
                     NTAAVQNPDLVFDASSKVGSQCIVVAIDAKQVAPGKWH"
     gene            complement(421..1161)
                     /gene="hisA"
                     /locus_tag="PLN02_12585"
     CDS             complement(421..1161)
                     /gene="hisA"
                     /locus_tag="PLN02_12585"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOI53209.1"
                     /translation="MLIIPAIDLKDGQCVRLKQGLMEQATVFSESPAEQARHWLEQGA
                     RRLHLVDLNGAFAGKPKNAAAIKAILAEVGDEIPVQLGGGIRDLDTIEACLDGGLSYV
                     IIGTAAVKNPGFLHDACTAFPGHIIVGLDAKDGKVATDGWSKLTGHDVVDLGKKYEDY
                     GVESIIYTDIGRDGMLSGINIEATVRLAQALTIPVIASGGLSNLDDIRKLCAVEGEGV
                     VGTIAGRAVYDGSLDFKAAQELADELGA"
     gene            complement(1202..1843)
                     /gene="hisH"
                     /locus_tag="PLN02_12590"
     CDS             complement(1202..1843)
                     /gene="hisH"
                     /locus_tag="PLN02_12590"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOI53210.1"
                     /translation="MAGRTIAVIDYGMGNLRSAAKAVEHVAAGATVVVTADPAVVAAA
                     ERVVFPGQGAMPDCMRELDARGLREAVLQAAREKPFLGICVGEQMLFEKSEEGDVAGL
                     GVFPGVVRRFPEARMVDANGQRLKVPHMGWNEVQQRPHALWAGIADASRFYFVHSYCV
                     QDIDPALTVGVTDYGLPFTCAVARDNIFAVQFHPEKSARDGLQLLKNFVEWRP"
BASE COUNT          317 a          591 c          624 g          318 t
ORIGIN      
        1 catgccactt gcccggcgcc acctgcttgg cgtcgatggc gacgacgatg cactgcgagc
       61 cgaccttgct cgacgcgtcg aaaaccagat ccgggttctg caccgcggcg gtgttcatcg
      121 acaccttgtc ggcgccggcg ttgagcaggc gacgcacgtc ttccaccttg cgcacgccac
      181 cgccgacggt gagcgggatg aagacctgct cggcgcaggc ttcgacaatg tgcaggatga
      241 tgtcgcgccg gtcggacgac gccgtgatgt cgaggaaggt gacctcgtcg gcgccctgct
      301 cgttgtaacg gcgggcgatc tcgatcggat cgccggcatc gcgcaggccg acgaagttgg
      361 tgcccttgac gacccggccg gcggtgacgt cgagacaggg aatgatgcgt ttggcgagca
      421 tcaggcgccc agctcgtcgg cgagttcctg tgccgccttg aagtcgagcg atccgtcgta
      481 gacggcgcga ccggcgatgg tgccgacaac gccttcgcct tcgaccgcgc acagcttgcg
      541 gatgtcgtcg aggttggaca ggccaccgga agcgatcacc ggaatggtca gcgcctgcgc
      601 cagacgcacg gtggcttcga tgttgatgcc ggagagcatg ccgtcgcggc cgatgtcggt
      661 atagatgatc gattcgaccc cgtaatcctc gtacttcttg cccaggtcca cgacgtcgtg
      721 accggtcaac ttcgaccagc cgtcggtcgc caccttgccg tccttggcgt cgaggccgac
      781 gatgatgtgg ccgggaaagg cggtgcaggc gtcgtgcagg aaaccggggt tcttcaccgc
      841 cgcggtgccg atgatgacat aggacagacc gccgtcgaga caggcctcaa tggtatcgag
      901 atcgcggatg ccgccgccga gctgaaccgg aatctcgtcg ccgacttcgg ccaggatcgc
      961 cttgatcgct gcggcgttct tcggtttgcc ggcaaaggcg ccattgagat cgaccagatg
     1021 caggcgccgc gcgccctgct caagccagtg ccgggcctgt tcggcgggac tctcggagaa
     1081 aacggtggcc tgttccatca ggccctgttt gaggcggacg cattgtccgt ccttcaggtc
     1141 gattgcggga atgatcagca tgggaaaaag tctgcgaggg ggaatttccg ggtggcgccg
     1201 ttcacgggcg ccattcgacg aagttcttca ggagctgcag gccgtcgcgg gcgcttttct
     1261 cgggatggaa ctggacggca aagatattat cccgcgccac cgcacaggta aagggcaggc
     1321 cgtagtcggt gacgccgacg gtcagcgccg ggtcgatgtc ctgcacgcag tagctgtgca
     1381 cgaagtagaa gcgcgatgcg tcggcgattc ccgcccacag cgcatgcggc cgctgctgca
     1441 cttcgttcca gcccatgtgc ggcaccttca ggcgctggcc gttggcatcg accatgcgcg
     1501 cctcggggaa acgccggacg acgccgggaa agacgccgag accggcaaca tcgccttcct
     1561 cgctcttttc gaaaagcatc tgttcgccga cgcagatgcc gaggaagggt ttctcacgcg
     1621 ccgcctgcag caccgcctcg cgcaagccac gggcgtcgag ctcgcgcatg caatcgggca
     1681 tcgccccctg gccggggaag acgacgcgct cggcggcggc gaccaccgcc ggatcggcgg
     1741 tgacgacgac ggtggcgcct gcggcaacat gctcgaccgc cttggcggcc gagcgcagat
     1801 tgcccatgcc gtagtcgatg acggcgatcg tccggccagc catcacagcg
//
LOCUS       DAOSDX010000646         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21438_length_1848_cov_3.145008, whole genome shotgun sequence.
ACCESSION   DAOSDX010000646 DAOSDX010000000
VERSION     DAOSDX010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21438_length_1848_cov_3.145008"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="PLN02_12595"
     CDS             complement(<1..106)
                     /locus_tag="PLN02_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOI53211.1"
                     /translation="MTQPVVMYTTAVCPYCMRAKQLLKARGVAAIEEVR"
     gene            complement(103..528)
                     /locus_tag="PLN02_12600"
     CDS             complement(103..528)
                     /locus_tag="PLN02_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI53212.1"
                     /translation="MPMDFINQNILLIGIVVVSGLGLLWPLVFRSQGKAVNPGEATLM
                     INRQDAKVIDVRQASEFAAGHIPDAVHIPEAQLGERLAELEKFKAQPLILCCASGLRS
                     DRACKALKQHGFEQLFNLGGGVDAWVAAGYPLHKGGRKK"
     gene            complement(532..849)
                     /locus_tag="PLN02_12605"
     CDS             complement(532..849)
                     /locus_tag="PLN02_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOI53213.1"
                     /translation="MDLISSALIDKQEHIETAAKALKAIAHPLRLKILCVVGDQEACV
                     QDIVEAVGTSQSNISQHLAILREKGVLLTRKDANRVFYRVGDQRTLQLVGMMREVFCG
                     VEN"
     gene            929..>1848
                     /locus_tag="PLN02_12610"
     CDS             929..>1848
                     /locus_tag="PLN02_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HOI53214.1"
                     /translation="MLSARAGLRSAFSACSLALLLALSLISPATAQKKASAPEVAAKR
                     ADLEELRNRIEDLRKDLSSSEDSRADAADRLRESERQISRLQRKLHELGGQREQLQSR
                     LENLETQSRELGGTLTLQQQQLENLLYRQYLKGSPDSLQLLLNGDNPSQLARDLYYLS
                     AIAGAKAELMSEIQATIRQKKALAEDTRERTAELAEVENEQRRHHTELEQQRRERQAL
                     HAELSSKIRAQRREIGSLQRDEKRMTRLIDRLSRILAQQAARAAEEARRKPPPPPANA
                     GNSREPAAPRESPGKENRYDPVPNDGRFAKL"
BASE COUNT          395 a          632 c          528 g          293 t
ORIGIN      
        1 cccggacctc ctcgatcgcc gccaccccgc gcgccttcag caactgcttg gcgcgcatgc
       61 agtacgggca gaccgccgtg gtgtacatca cgaccggctg cgtcatttct tgcgtcctcc
      121 cttgtgcagc ggatagcccg ccgcgaccca ggcatcgacc ccgccgccga ggttgaacag
      181 ctgttcgaag ccatgctgct tcagtgcctt gcaggcccgg tcggaacgca ggccggaagc
      241 acagcacagg atcagcggct gcgccttgaa tttttccagc tccgccaggc gctcaccgag
      301 ctgcgcttcc ggaatgtgaa ccgcatccgg aatgtgtccg gcggcaaact cgctcgcctg
      361 acgcacatcg atcaccttgg cgtcctggcg gttgatcatc agcgtcgcct cgcctggatt
      421 gacggccttg ccctgcgacc ggaagacgag cggccagagc agcccgagac cggaaacgac
      481 aacgatcccg atgagcagga tgttctggtt gatgaaatcc atcggcattg cttaattctc
      541 cacaccacaa aaaacttcgc gcatcatgcc gacaagctgc agggtgcgct gatcgccgac
      601 acggtagaac acccggttgg catccttgcg cgtcagcagg acccccttct cgcgcaggat
      661 cgcgagatgc tgtgagatgt tgctttgcga agtgccgacg gcttcgacga tatcctgcac
      721 gcaggcttcc tgatcgccga caacgcacag gatcttcagg cgcaggggat gcgcgattgc
      781 cttcaaggct ttcgccgccg tctcgatatg ctcctgtttg tcgatcagag cggaggaaat
      841 aaggtccaag atataaaacc tcagtttggt tgaacggttg attatagaat aagccttgcc
      901 aaacctagaa cccaatcacg atttccccat gctttccgcc cgcgccggcc tgcgctccgc
      961 tttttccgcc tgctcgctcg cgcttctgct ggcgctctcc ctgatctctc ccgccacagc
     1021 ccagaagaag gccagcgcgc cggaagtggc ggccaaacgc gccgacctcg aggaattgcg
     1081 caaccgcatc gaagacctgc gcaaggacct gtcgagcagc gaagactccc gcgccgacgc
     1141 cgccgaccgg ctacgtgaat cggagcggca gatttcccgc ctgcaacgca agctgcacga
     1201 actgggcggg caacgcgagc aattgcagag ccggctggaa aacctggaaa cccagtcacg
     1261 cgaactgggc ggcacgctga ccctgcagca acagcagctg gaaaacctgc tctatcgcca
     1321 gtacctgaaa ggctcgcccg attcgctaca gttgctgctc aacggcgaca acccgagcca
     1381 gctggcgcgc gatctttatt atctgtccgc catcgccggt gccaaggctg aactgatgag
     1441 cgaaatccag gccaccatcc ggcagaagaa ggcgctcgcc gaggacaccc gcgaacgcac
     1501 cgccgaactg gctgaagtcg aaaacgaaca gcgccggcac cacaccgaac tcgagcagca
     1561 acgtcgcgaa cgccaggcac tgcacgccga actatcgagc aagatccgcg cacaacggcg
     1621 ggagatcggc tcgctgcagc gcgacgagaa acggatgacc cggctgatcg accgactgtc
     1681 gcggatactt gcccagcagg cggcccgggc ggcggaagaa gcccgccgca aaccgccgcc
     1741 accgcccgcc aatgccggca acagccgcga accggcggcg ccgcgcgaaa gcccgggcaa
     1801 ggaaaaccgc tacgacccgg tacccaacga tggccgcttc gccaaact
//
LOCUS       DAOSDX010000647         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21452_length_1847_cov_3.547433, whole genome shotgun sequence.
ACCESSION   DAOSDX010000647 DAOSDX010000000
VERSION     DAOSDX010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21452_length_1847_cov_3.547433"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..148
                     /locus_tag="PLN02_12615"
     CDS             14..148
                     /locus_tag="PLN02_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOI53215.1"
                     /translation="MHNVFREACTGCSACIEKCPTEALLMKPVPVTLQHWVMPKPLTA
                     "
     gene            160..1704
                     /gene="rsxC"
                     /locus_tag="PLN02_12620"
     CDS             160..1704
                     /gene="rsxC"
                     /locus_tag="PLN02_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOI53216.1"
                     /translation="MGFMNLFKHVLGDDWGVHPEDHKRPAADRPVRTMPVPARLFLPL
                     QQHLGAPARPVVLVGQKVKKGELIAEAQGMISAPLHAPTSGSIAAVTEITAPHPSGLT
                     LQAIILEADGLDEWGELHGCADPFALDPAEIGKRVAAAGVVGLGGAAFPSAVKLNGAS
                     RSKVTTLIMNGGECEPYLSCDDRQMRDHAAAIVDGIRIMLHATGAAVALVGIEDNKPE
                     AIAAMQAAAAGVDKVEIRPVPARYPMGSEKQLVQVLTGKEVPADGRTFDIGVIVHNVG
                     TALAVRAAIREGKPLISRLLTVNGNCASEPGNIEVRIGTLAEEVLAFAGGLKGDGLGL
                     ARRVLGGPMMGMQIPHWRVPVVKGSSGILALDADEVVARDPNPCIRCGSCVKACPMGL
                     LPLEMSARIRSEAYDEAVAYGLKDCIACGCCAYVCPSQIPLVQYFVHAKGELAAQDRS
                     KLRNDATKKLALQRQERLDREARERAEAAAKRKAEREAAAAAKAAAEAATKAASASAA
                     EGQGVI"
BASE COUNT          315 a          619 c          637 g          276 t
ORIGIN      
        1 cgccgccaag caggtgcata acgtcttccg cgaggcctgc accggttgct cggcctgcat
       61 tgagaaatgt ccgaccgagg cgctgctcat gaagccggtc ccggtcaccc tgcagcattg
      121 ggtgatgccc aagccgctga ctgcgtgaga gcgaaaacca tgggcttcat gaatctgttc
      181 aagcatgtcc tcggcgacga ctggggcgtc catccggaag atcacaagcg gccggcggcc
      241 gatcgcccgg tgcgcacgat gccggtgccg gccaggctct tcctgccgct gcagcagcac
      301 cttggcgcgc cggcacggcc ggtcgtgctg gtcggccaga aggtgaagaa gggcgagctg
      361 atcgccgagg cgcagggcat gatctcggca ccgctgcacg cgccgacctc gggcagcatc
      421 gctgccgtca ccgaaatcac cgccccgcat ccctccggtc tgaccttgca ggcgatcatc
      481 ctggaggccg acggccttga cgaatggggc gagctgcacg gctgcgccga tcccttcgcg
      541 ctggacccgg cggaaatcgg caagcgggtc gccgccgccg gcgtcgtcgg cctcggcggt
      601 gccgccttcc cgtcggcggt caagctcaac ggcgcctcgc gttccaaggt gacgacgctg
      661 atcatgaacg gcggcgagtg cgaaccctac ctgtcctgcg acgaccgcca gatgcgcgac
      721 cacgcggcgg cgatcgtcga tggcatccgc atcatgctcc acgccaccgg cgccgccgtc
      781 gccctggtcg gcatcgagga caacaagccg gaagccatcg ccgccatgca ggcggcggca
      841 gccggcgtcg acaaggtcga aatccggccg gtaccggcgc gctacccgat gggttcggaa
      901 aagcagctgg tgcaggtgct gaccggcaag gaagtgccgg ccgatgggcg taccttcgac
      961 atcggcgtca tcgtccataa cgtcggcacc gcgctcgccg tgcgcgccgc gatccgcgaa
     1021 ggcaagccgc tgatctcgcg cctgctgacg gtcaacggca actgcgccag cgaaccgggc
     1081 aacatcgagg tgcgcatcgg caccctggcc gaggaagtgc tggccttcgc cggcggcttg
     1141 aagggcgacg gccttgggct ggcccgccgg gtgctcggtg ggccgatgat gggcatgcag
     1201 attccgcact ggcgggtgcc ggtggtcaag ggcagcagcg gcatcctggc gctcgacgcc
     1261 gacgaggtcg tggcccgcga cccgaacccg tgcatccgct gcggctcctg cgtcaaggcc
     1321 tgcccgatgg gcctgctgcc gctcgaaatg agcgcccgca tccgcagcga agcctacgac
     1381 gaagcggtcg cctacggtct caaggattgc atcgcctgcg gctgctgcgc ctatgtctgc
     1441 ccgtcgcaga ttccgctggt ccagtatttc gttcatgcca agggcgaact cgccgcccag
     1501 gatcgcagca agctgcgcaa cgacgcgacc aagaaactcg ccctgcaacg ccaggaacgc
     1561 ctcgaccgcg aagcccgcga acgggcggaa gcggcggcca agcgcaaggc cgaacgggaa
     1621 gccgcggcgg cggccaaggc ggcggcggaa gccgcgacca aggcggcgtc ggcttcggca
     1681 gccgaggggc agggggtgat atgagattgg gggcttgcgc cttgcttgag ttggcggatt
     1741 ccgcgcctga cgcgcgggcg cgcttggttg ttgaaccgct gttttcggcc gctgaccggc
     1801 gggcgtactt tcttttttgt ggaaaaaaga aagtagccaa agaaaaa
//
LOCUS       DAOSDX010000648         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21486_length_1845_cov_3.941899, whole genome shotgun sequence.
ACCESSION   DAOSDX010000648 DAOSDX010000000
VERSION     DAOSDX010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21486_length_1845_cov_3.941899"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /gene="ubiG"
                     /locus_tag="PLN02_12625"
     CDS             <1..376
                     /gene="ubiG"
                     /locus_tag="PLN02_12625"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HOI53217.1"
                     /translation="QMPGAFDTVTCLEMLEHVPNPSSTITACARLVKPGGQVFFSTLN
                     RNPKAYLFAVIGAEYLLKLLPKGTHDYAKFIKPSELTRWAKMGNLEPQEILGMSYNPL
                     TREYSLGRDTSVNYLLRAQRNA"
     gene            369..1037
                     /gene="gph"
                     /locus_tag="PLN02_12630"
     CDS             369..1037
                     /gene="gph"
                     /locus_tag="PLN02_12630"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921771.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOI53218.1"
                     /translation="MPETAPRAVLFDLDGTLADTAPDLAACVNRLLVEEGRAPQPLVE
                     LRPFTSQGVRGMLFKGFGMSPEHPDYAAFAARFLAHYETRLCEETRLFDGIADLLDRL
                     EENGLRWGIVTNKRMRYTDPLVRLLGLAPRTTCVVSGDTTAAAKPSPLPLLHACELLG
                     VAPSATLYVGDDQRDIVAGQAAGCATIAVRWGYLGVERPIEAWGADRIVDHPREIAAV
                     SRPT"
     gene            complement(1021..>1845)
                     /locus_tag="PLN02_12635"
     CDS             complement(1021..>1845)
                     /locus_tag="PLN02_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI53219.1"
                     /translation="GRGIAAQGPLPPGIFGYEAGEAGINRVVYDWVEGQARRKSAAVA
                     KQLLAEAGYPNGRDARNGEPLVLNLDTTGGGMGEKSRLDWLTRQFAKIDVQLVVRATD
                     FNRFQDKLRKGAFQLFYLGWNADYPDPENFMFLLHGPEGRLQGGANSANYENPEYDRL
                     FLQMKNMENSPERLAIIRRMNDILRHDAPWVFGQHPKSYTLTHRWLKNRKPSDVGNNT
                     LKYQRVDAVDRAQARREWNAPVRWPLLAALGVLLLAVVPALIGYRRRERAAVRSAD"
BASE COUNT          285 a          681 c          578 g          301 t
ORIGIN      
        1 acagatgccc ggcgccttcg acacggtcac ctgcctggaa atgctcgaac acgtgcccaa
       61 tccgtcgagc accatcaccg cctgcgcccg cttggtcaaa cccggcggcc aggtcttctt
      121 ctcgacgctc aaccgcaacc cgaaggccta cctgttcgcg gtgatcggcg ccgagtacct
      181 gctcaagctg ctgcccaagg gcacccacga ttacgccaag ttcatcaagc cgtcggaact
      241 cactcgctgg gccaagatgg gcaacctgga accgcaggaa atcctcggca tgagctacaa
      301 cccgctgacc cgggaataca gcctcggccg cgacaccagc gtcaattacc tgctgcgcgc
      361 ccagcgcaat gcctgagacc gctccccggg ccgtcctttt cgacctcgac ggcacgctcg
      421 ccgacaccgc ccccgacctc gccgcctgcg tcaatcgcct gctggtcgag gaaggccggg
      481 cgccgcagcc gctcgtcgag ctgcgcccgt tcacctcaca gggcgtgcgc ggcatgttgt
      541 tcaagggttt cggcatgagc cccgagcacc ctgactacgc cgccttcgcg gcgcgcttcc
      601 tggcccacta cgaaacccgc ctgtgcgagg aaacgcgcct tttcgacggc attgccgacc
      661 tgctcgaccg gcttgaggag aacgggctgc gctggggcat cgtcaccaac aagcggatgc
      721 gctacaccga cccgctggtc cgcctgctcg gcctggcgcc gcgcacgacc tgcgtcgtca
      781 gcggcgacac cacggccgcc gccaagccat cgccgctgcc cctgctgcat gcctgcgaac
      841 tgctcggggt ggcgccgtcc gccaccctct acgtcggcga cgaccagcgc gatatcgtcg
      901 ccggccaggc cgccggctgc gcgacgatcg ccgtgcgctg gggctacctc ggcgtcgagc
      961 gcccgatcga ggcctggggc gccgaccgca tcgtcgacca cccgcgggaa atcgccgccg
     1021 tcagtcggcc gacctgaccg ccgcccgctc gcgccgccgg taaccgatca aggccggcac
     1081 caccgccagc agcagcactc cgagcgccgc cagcagcggc cagcgcaccg gcgcattcca
     1141 ctcacgccgc gcctgcgccc ggtcgacggc gtcgacccgc tggtacttca gcgtgttgtt
     1201 gccgacatcg ctcggcttgc ggttcttcag ccagcggtgg gtcagcgtgt agctcttcgg
     1261 gtgctgcccg aaaacccagg gcgcgtcgtg gcgcaggatg tcgttcatgc gccggatgat
     1321 ggccaggcgc tcgggcgagt tctccatgtt cttcatctgc aggaacagcc ggtcgtactc
     1381 gggattctcg tagttggccg agttggcgcc gccttgcaga cgcccttccg gaccgtgcag
     1441 caggaacatg aagttctccg gatcggggta gtcggcattc cagccgaggt agaagagctg
     1501 gaaggcgccc ttgcgcagct tgtcctggaa gcggttgaag tcggtggcgc gcaccaccag
     1561 ttgcacgtcg atcttggcga actgccgggt cagccagtcg aggcgcgatt tttcacccat
     1621 tccgccgccg gtggtgtcga ggttgagaac cagcggctcg ccgttcctgg cgtcgcgccc
     1681 attcgggtag ccggcttcgg cgagcagctg tttggcgacg gcggccgact tgcgccgcgc
     1741 ctgcccttcg acccagtcat agacgacgcg gttgattccc gcctcgcccg cttcgtagcc
     1801 gaagatgccg ggcggcagcg gcccctgagc ggcgatgccg cgacc
//
LOCUS       DAOSDX010000649         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21508_length_1844_cov_4.475685, whole genome shotgun sequence.
ACCESSION   DAOSDX010000649 DAOSDX010000000
VERSION     DAOSDX010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21508_length_1844_cov_4.475685"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /locus_tag="PLN02_12640"
     CDS             <1..1805
                     /locus_tag="PLN02_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOI53220.1"
                     /translation="AVIDLAEQREARMQPAARKLLAMWPDMQRAYAGDEYVVKIRDRE
                     IRTRLTHTTLSGTTLRKVVLPHYADHGEVLRWLMRDNVPGSFPYTAGTFAFKRENEDP
                     TRMFAGEGDAFRTNRRFKMLSEGMPAKRLSTAFDSVTLYGADPDRRPDIYGKIGNSGV
                     SIATLDDMKVLYDGFDLVCPTTSVSMTINGPAPIILACFFNTAIDQQIAKFEKDNGRQ
                     PTEDEAEKIREWVLKSVRGTVQADILKEDQGQNTCIFSTEFALKMMGDIQEFFVHNQV
                     QNFYSVSISGYHIAEAGANPISQLAFTLSNGFTYVESYLARGMHIDDFAPNLSFFFSN
                     GMDPEYSVIGRVARRIWAVAMKNKYGANERSQKLKYHIQTSGRSLHAQEMDFNDIRTT
                     LQALIAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINREWGVAKNENPNQGAF
                     VIDELTDLVEEAVLKEFEAIASRGGVLGAMETGYQRGKIQEESMYYEHKKHDGSYPII
                     GVNTFLNPKGMAQQEIELARSTDEEKQSQIQRLRDFHARNADRAPAMLEKLKQTVIED
                     GNVFAVLVDAVKCCSLGQISSALYEVGGQYRRSM"
BASE COUNT          402 a          611 c          539 g          292 t
ORIGIN      
        1 aggccgtgat cgatctggcc gaacagcgcg aagcccgcat gcagcccgcc gcgcgcaagc
       61 tgctggcgat gtggcccgac atgcagcgcg cctacgccgg tgacgaatat gtggtgaaaa
      121 ttcgcgaccg cgaaatccgc actcgcctca cccacaccac gctgtcgggc accacgctgc
      181 gcaaggtggt gctgccgcac tatgccgatc atggcgaggt gctgcgctgg ctgatgcgcg
      241 acaacgtgcc gggcagcttc ccgtacaccg cgggcacttt tgccttcaag cgggagaatg
      301 aagatcccac ccgcatgttc gccggcgagg gcgatgcctt tcgcaccaac cggcgcttca
      361 agatgctgag cgagggcatg ccggccaagc gcctgtccac cgcgttcgac tccgtcacgc
      421 tttacggtgc agatcccgac cgccgcccgg acatctacgg caagatcggc aactccggtg
      481 tctcgattgc cacgctcgac gacatgaagg tgctctacga cggcttcgac ctggtctgcc
      541 cgacgacctc ggtgtcgatg acgatcaacg gcccggcgcc gatcatcctg gcctgcttct
      601 tcaacaccgc gatcgaccag cagatcgcca agtttgaaaa agacaacggc cgccagccga
      661 ccgaggacga agccgagaag attcgcgaat gggtgctcaa gtcggtgcgc ggcaccgtcc
      721 aggccgacat cctgaaggag gatcagggcc agaacacctg catcttctcg accgaattcg
      781 cgttgaagat gatgggcgac atccaggaat tcttcgtcca taaccaggtg cagaacttct
      841 actcggtgtc gatctccggc taccacatcg ccgaagccgg ggccaacccg atctcgcagc
      901 tcgccttcac gctgtccaac ggcttcacct acgtggaaag ttatttggcg cgcggcatgc
      961 acatcgacga ttttgccccg aacctctcct tcttcttctc caacggcatg gatccggaat
     1021 actcggtgat cggccgcgtc gcccgccgca tctgggcggt ggcgatgaag aacaagtacg
     1081 gcgccaacga gcgcagccag aaactcaagt accacatcca gacctccggc cgctcgctgc
     1141 acgcgcagga gatggacttc aacgacatcc gcaccacgct gcaggcgctg atcgccatct
     1201 acgacaactg caactcgctg cacaccaacg cctacgacga agcgatcacg acgccgaccg
     1261 aggagtccgt gcgccgcgcc atggccatcc agttgatcat caaccgcgag tggggcgtgg
     1321 cgaaaaacga gaacccgaac cagggcgcct tcgtcatcga cgagctgacc gatctggtcg
     1381 aggaagccgt gctcaaggaa ttcgaagcca tcgcctcgcg cggcggcgtg ctcggcgcca
     1441 tggagaccgg ctaccagcgc ggcaagatcc aggaagagtc gatgtactac gagcacaaga
     1501 agcacgacgg ctcctacccg atcatcggcg tgaacacctt cctcaatccg aagggcatgg
     1561 cgcagcagga aatcgaactg gctcgctcga ccgacgagga aaagcaatcg caaatccagc
     1621 gcttgcgcga cttccacgcc cgcaacgccg accgtgcgcc ggccatgctg gagaagctca
     1681 agcagacggt gatcgaggac ggcaacgtct tcgccgtgct ggtcgatgcg gtgaagtgct
     1741 gctcgctggg tcagatcagc tcggcgctct acgaagtcgg cggccagtac cgcagaagca
     1801 tgtaagcaag gccccgaaag gggaacagca atccggaccc gaac
//
LOCUS       DAOSDX010000650         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21528_length_1843_cov_2.584452, whole genome shotgun sequence.
ACCESSION   DAOSDX010000650 DAOSDX010000000
VERSION     DAOSDX010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21528_length_1843_cov_2.584452"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLN02_12645"
     CDS             <1..317
                     /locus_tag="PLN02_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002925759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOI53221.1"
                     /translation="LNSVGSASNPMFAMPWHWHLVLGGFAFGMVFMATDPVSAAMTDT
                     GKWIFGALIGVMVVLIRVVNPAFPEGMMLAILFANLCAPLIDHFVVTANIKRRAARHV
                     QQ"
     gene            304..1089
                     /locus_tag="PLN02_12650"
     CDS             304..1089
                     /locus_tag="PLN02_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HOI53222.1"
                     /translation="MSSNKESTVRTLLVALTVSLVASVFVAGSAVALKPVQLENRLLD
                     KQRSILAIAGLGDNLSSAQVKEVFGSRIKARVVDLDTGEYQTAFDPLGFDPLKAAKDP
                     KMSAALSSEADKALIKRRERFTTVYMVEQEGRIESLILPIRGYGLWSTLHGFLALKAD
                     LNTVVGLGFYQHAETPGLGGEVDNPKWKALWPGKTLFDDAGRPIIKVVKGGVDPASPD
                     AGHQVDALAGATLTSNGVDRLLQFWLGEQGFGPYLARLRQQGV"
     gene            1092..>1843
                     /locus_tag="PLN02_12655"
     CDS             1092..>1843
                     /locus_tag="PLN02_12655"
                     /inference="COORDINATES: protein motif:HMM:NF006777.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOI53223.1"
                     /translation="MAQQATVKEVLFNPVFNNNPIALQILGICSALAVTSNLKTALVM
                     AIALTLVTAFSNLFISLIRAQIPGSIRMIVQMVIIASLVIVVDQVLRAYAFSLAKQLS
                     VFVGLIITNCIVMGRAEAFAMQNPPMLSFWDGIGNGLGYSTMLVALGVIRELFGAGKL
                     LGIEILAADIEYYLGQLGLKVVGRKPRPVGDSDATQPVTFAIPTFRPDLKREADLIEE
                     VCRLHGVDKIPAVLPRGARGASEYDPIHDQLA"
BASE COUNT          314 a          602 c          572 g          355 t
ORIGIN      
        1 tgctcaacag tgtcggttcg gccagcaatc cgatgttcgc catgccctgg cactggcatc
       61 tggtgctcgg cggcttcgcg ttcggcatgg tgttcatggc caccgatccg gtgtcggcgg
      121 cgatgactga taccggcaaa tggattttcg gggcgctgat cggcgtcatg gtggtgctca
      181 tccgcgtggt caatccggct ttcccggagg gcatgatgct ggcgatcctg ttcgccaacc
      241 tctgtgcccc cttgatcgat cacttcgtgg tcacggccaa catcaagcgg agggcggcgc
      301 gccatgtcca gcaataagga atccacggtc cgcaccctgc tcgtcgcgtt gaccgtcagc
      361 ctcgtcgctt cggttttcgt ggccggttcg gcggtggcgc tgaagccggt ccagctcgag
      421 aaccgcctgc tcgacaagca gcgcagcatc ctggcgatcg ccggcctggg cgacaacctc
      481 tcgtcggcac aggtcaagga ggttttcggc agccgcatca aggcgcgcgt ggtcgatctc
      541 gataccggcg agtatcagac ggcgttcgat ccgctcggtt tcgatccgct gaaggctgcc
      601 aaggacccga agatgtccgc cgcactcagc agcgaggcgg acaaggcgct gatcaagcgg
      661 cgcgaacgct tcaccaccgt gtatatggtc gagcaggaag gccggatcga atcgctgatc
      721 ctgccgatcc gcggctacgg tctgtggtcg acgctgcacg gcttcctcgc gctgaaggcc
      781 gacctcaaca ccgtggtcgg cctgggcttc taccagcatg ccgaaacccc gggtctcggc
      841 ggcgaagtgg acaatccgaa atggaaggcg ctgtggccgg gcaagacgct gttcgacgat
      901 gccggccggc cgatcatcaa ggtggtcaag ggcggggtcg atccggccag cccggatgcc
      961 ggccatcagg tcgatgcgct ggccggtgcg acgctgacca gtaacggcgt cgaccgattg
     1021 ttgcaattct ggctaggcga gcagggcttc ggcccctacc tcgccaggct gcgtcagcag
     1081 ggagtctgag catggcacaa caagctaccg tcaaggaagt cctgttcaat ccggtcttca
     1141 acaacaatcc gatcgccttg cagatcctcg gcatctgctc ggcgctggcc gtcacctcca
     1201 acctcaagac ggcgctcgtc atggccatcg cgctgacgct ggtgacggcc ttctccaacc
     1261 tgttcatctc gctgatccgg gcgcagatcc cggggtcgat ccggatgatc gtgcagatgg
     1321 tgatcatcgc ttcgctggtc atcgtcgtcg atcaggtgct gcgcgcctac gccttcagcc
     1381 tggccaagca gctatcggtg ttcgtcggcc tgatcatcac caactgcatc gtcatgggcc
     1441 gggccgaagc tttcgccatg cagaatccgc ccatgctgtc gttctgggac gggatcggca
     1501 acggcctcgg ttacagcacg atgctcgtcg cgctcggcgt catccgcgaa ctgttcggcg
     1561 ccggcaaact gctcggcatc gagatcctcg cggcggacat cgagtattat ctcggccagc
     1621 tcgggctcaa ggttgtcgga cgcaaacccc ggccggtagg cgactccgat gcgacccagc
     1681 cggtcacctt tgccattccg acgttccgcc ccgacctcaa acgtgaagcg gatctcatcg
     1741 aggaggtctg tcggctccat ggcgtcgaca agatccccgc cgtgctccct cgaggcgccc
     1801 gcggcgccag cgaatacgat ccgatccacg accagcttgc cca
//
LOCUS       DAOSDX010000651         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21540_length_1842_cov_3.543369, whole genome shotgun sequence.
ACCESSION   DAOSDX010000651 DAOSDX010000000
VERSION     DAOSDX010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21540_length_1842_cov_3.543369"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLN02_12660"
     CDS             complement(<1..278)
                     /locus_tag="PLN02_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOI53224.1"
                     /translation="MDDALNTFVAEGRELLEQMEESLLQIEQAPDDAGLINAIFRAAH
                     TIKGSAGIFGIDYVVAFTHVAENVLDRVRAGNLTINSDLVALLLAVRD"
     gene            complement(301..609)
                     /locus_tag="PLN02_12665"
     CDS             complement(301..609)
                     /locus_tag="PLN02_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI53225.1"
                     /translation="MAKKPTQRRLELSGEMSISTAGAIRQQLLEALSGAEIVEVDLGR
                     VSECDTTGVQLMLAAKRQAAQAGKTLIFADHSAPVVETLDLLDLFAQLGDPVLIRSQV
                     "
     gene            complement(618..986)
                     /locus_tag="PLN02_12670"
     CDS             complement(618..986)
                     /locus_tag="PLN02_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53226.1"
                     /translation="MGKSIMIVDDSASLRQVVGIALKGAGYDVTEACDGKDALGKLDG
                     RKFHLIISDVNMPNMDGITLLKALKQLAAYKFTPVIMLTTEAGEDRKREGQAAGARAW
                     VVKPFQPAQMLAAVSKLILP"
     gene            complement(1004..>1842)
                     /locus_tag="PLN02_12675"
     CDS             complement(1004..>1842)
                     /locus_tag="PLN02_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOI53227.1"
                     /translation="ALADQFSGISADLTGTLASTPEDNLMNQLLGDAQSKLEGIIDGL
                     SQALAHRNAMLEKATSMAGNTEQLRQMANEVAEIASQTNLLALNAAIEAARAGEAGRG
                     FAVVADEVRKLSTKSGDTGRKITRTVDLVNADIVETLNASKSFIAQDESLIEASSRAI
                     GEVVSSIKASAQRLSASSEALRAQGYATNNAIAEILVALQFQDRVSQVLGHVVADIER
                     MTSNLAEQEALLRQGLVPESIDVSSWLEELASTYTVPEQHLIHRGKAAAGNAPAAEIT
                     FF"
BASE COUNT          302 a          582 c          589 g          369 t
ORIGIN      
        1 tgatcgcgga ccgccagcag cagggcaaca agatcggaat tgatggtcag gttgccggcg
       61 cgcacgcgat ctaggacatt ttccgcgacg tgggtgaagg cgacgacata gtcgatcccg
      121 aagatcccgg ccgatccctt gatcgtgtgc gcggcccgga agatcgcgtt gatcagtccg
      181 gcgtcgtcgg gcgcctgttc gatctgcagc agcgactcct ccatctgttc cagaagttcc
      241 ctgccttcgg caacgaaggt attcagcgcg tcgtccatgg tagacatgtg ggcatcctcg
      301 tcagacttgg gaacggatca ggaccggatc gccgagctgg gcgaacaggt cgagcaggtc
      361 gagcgtttcg acgaccggcg cgctgtggtc ggcaaagatc aaggtcttgc ccgcctgcgc
      421 ggcctggcgc tttgccgcca gcatcaactg gacgcccgtg gtatcgcatt cgctgacgcg
      481 gccgaggtcg acttcgacga tctcggcccc ggaaagcgcc tccaggagtt gttggcgaat
      541 ggcgcccgcc gtcgaaatgc tcatttcgcc gctcagttcg aggcgccgct gggtaggttt
      601 cttggccatc gctcgactca gggaaggatc agcttggaaa ccgccgccag catctgcgcc
      661 ggctggaaag gcttgaccac ccaggcccgg gcacccgccg cctgaccttc gcgcttcctg
      721 tcctcgcccg cttcggtggt cagcatgatg accggcgtga acttgtaggc agccagttgc
      781 ttgagcgcct tgagcagcgt gatgccgtcc atgttcggca tgttgacgtc gctgatgatc
      841 aggtgaaact tgcgcccgtc gagcttgccg agggcatcct tgccgtcgca agcctcggtg
      901 acgtcgtaac ccgccccctt gagggcgatc ccgacgacct ggcgcaagga cgcggagtcg
      961 tcgacgatca tgattgattt gcccatgatg gaaacctcgt ctgtcaaaag aaagtgatct
     1021 cggccgccgg agcgttgcca gccgccgcct tgccgcgatg gatcaggtgt tgctcgggta
     1081 ccgtgtaggt gctggccagt tcctccagcc agctcgagac atcgatcgat tcgggaacca
     1141 agccctgccg cagcagcgct tcctgttcgg cgaggttgct cgtcatccgt tcaatgtcgg
     1201 cgacgacgtg gcccagcacc tggctgacgc gatcctggaa ctgcagggcg acgagaatct
     1261 cggcgatcgc gttgttggtg gcgtacccct gggcccgcag cgcctcggac gatgcggaca
     1321 agcgctgcgc cgacgccttg atcgacgaga cgacctcgcc gatcgcccga ctggaagctt
     1381 cgatcagcga ctcgtcctgg gcgatgaagg atttcgaggc gttcagcgtt tccacgatgt
     1441 cggcattgac cagatcgacc gtccgcgtga tcttccggcc agtgtcgccg gacttggtcg
     1501 acagtttgcg cacctcgtcg gcgaccacgg cgaatccccg ccccgcttct ccggcgcggg
     1561 cagcctcgat ggccgcgttg agggcgagca ggttggtctg actggcgatt tcggcgactt
     1621 cgttggccat ctgccgcagt tgctcggtgt ttcccgccat cgaggtcgcc ttttcgagca
     1681 tcgcgttgcg atgcgccagc gcctggctca ggccgtcgat gattccctcc agcttgctct
     1741 gggcatcgcc gagcaactga ttcatcaggt tgtcttccgg cgtactggcc agcgtccccg
     1801 tcagatcggc ggagatgccg gagaactgat cggccaacgc gg
//
LOCUS       DAOSDX010000652         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21575_length_1840_cov_2.439216, whole genome shotgun sequence.
ACCESSION   DAOSDX010000652 DAOSDX010000000
VERSION     DAOSDX010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21575_length_1840_cov_2.439216"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..263)
                     /locus_tag="PLN02_12680"
     CDS             complement(114..263)
                     /locus_tag="PLN02_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOI53228.1"
                     /translation="MGQKVNEWLFGTKRRQGVVDTMAKQTARTMGTKLGNQILRGVLG
                     GIFKK"
     gene            287..1186
                     /locus_tag="PLN02_12685"
     CDS             287..1186
                     /locus_tag="PLN02_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53229.1"
                     /translation="MNPGGSLTVRASFAENRVRIAGVVLERPAMTRLFVGQNPDAAVR
                     LIPHLYALCGEAQRVTARAALAAAEGRSAPCDAIDTRLLWLEVLHENLWRLLLDWPVA
                     CGLPAAREEFAAWRSARLGVDGNGATRRLIEETLCPLVERCRIVLDDRGVAPTWPQLD
                     VAAWLAWWRTPGSERPALASTADIAAAYLARVADVARAAAALEAGQAFPLAAAGGDGL
                     GVAQTLTARGVLTHAIALRDGKVARYHLEAPTDASFARPEKLAALLADRRYATAEAAR
                     QGLEAAILALDPCLPYQVEWQDA"
     gene            1179..1520
                     /gene="hypA"
                     /locus_tag="PLN02_12690"
     CDS             1179..1520
                     /gene="hypA"
                     /locus_tag="PLN02_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOI53230.1"
                     /translation="MHEMSLAEGIIELVEDTARRENAARVGLVVLEIGQLSSVEPEAL
                     RFCFDVVTRGGIADGAALEIVAVPGAGWCMKCAATVAISELYGACPVCGSHQVQPTAG
                     TEMRVREIAID"
     gene            complement(1517..>1840)
                     /locus_tag="PLN02_12695"
     CDS             complement(1517..>1840)
                     /locus_tag="PLN02_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53231.1"
                     /translation="VVEVAVVVRFAMRVIMSMVVLTMIVGVFALMDVFVHMRRAIGVR
                     MRVAVGVGNLAIVAVRMFVLVRVAVFMHRAVGMDMAVGVRPALDFHFAVTAAANCTHP
                     CPLVI"
BASE COUNT          292 a          659 c          624 g          265 t
ORIGIN      
        1 cggacgtgct ggcgttcgca tccgcgccgg cacccgaggg cggcagcggc gcggtgctgg
       61 tgttgctggt acgcggttga cgcaccgcca gcgcgcggct ccggcgctga cgctcacttc
      121 ttgaagatgc cgccgagcac gccgcgcagg atctggttgc ccagcttggt gcccatggtg
      181 cgcgcggtct gcttggccat ggtgtccacc acgccctggc ggcgtttggt gccgaacagc
      241 cattcgttca ccttctgccc catgccgcct tcctcggcct gcgcggatga acccgggcgg
      301 cagcctgacc gtccgcgcca gtttcgcgga aaacagggtg cgcatcgccg gcgtcgtcct
      361 cgaacggccg gcgatgacgc gcctcttcgt cggccagaat ccggacgccg cggtgcgcct
      421 gatcccgcac ctctacgcgc tgtgcggcga ggcacagcgc gtcacagcgc gcgccgccct
      481 ggccgccgcc gaaggccgca gcgctccctg cgacgcgatc gacacccgcc tgctgtggct
      541 ggaagtgctg cacgaaaacc tgtggcggct gctcctcgac tggccggtgg cctgcggcct
      601 gccagcggcg cgcgaggaat tcgccgcctg gcgcagcgcc cgtctcggcg tcgacggcaa
      661 cggcgccacc cgccgcctga tcgaggaaac gctgtgtccg ctggtcgagc gctgccggat
      721 cgtcctcgac gaccgtggcg tcgccccgac ctggccgcaa ctcgacgtcg ccgcctggct
      781 cgcctggtgg cgtacgccgg gaagcgaacg gccagccctg gccagtaccg ccgacattgc
      841 cgccgcctac ctggcgcgcg tcgccgacgt tgcccgggcg gccgccgcgc tcgaagccgg
      901 ccaagcattt ccgctcgccg ccgctggcgg cgacggactc ggcgttgccc agacgctgac
      961 cgcgcgcggt gtcctgacgc acgcgatcgc gctacgcgac ggcaaggtcg cgcggtatca
     1021 tctcgaagca ccgaccgatg cgagtttcgc ccggccggaa aaactcgcgg cgctgctcgc
     1081 cgaccgtcgc tacgcgacgg ccgaggcagc ccgccaggga ctggaggcgg cgatcctggc
     1141 gctcgatccc tgcctgcctt accaagtgga gtggcaagat gcatgaaatg tcgctggccg
     1201 aaggcatcat cgaactggtc gaagacaccg cacggcgcga gaacgccgcc cgggtcggct
     1261 tggtcgtgct cgagatcggc cagctgtcgt cggtcgaacc ggaagcgctg cgcttctgct
     1321 tcgacgtcgt cacccggggc ggcatcgccg acggtgcggc gctggagatt gtcgccgtgc
     1381 ccggcgccgg ctggtgcatg aaatgcgcgg caacggtagc aatcagcgag ctttacggcg
     1441 cctgcccggt ctgcggcagc caccaggttc aaccgacggc cggcaccgag atgcgggtgc
     1501 gggaaatcgc catcgactag ataacaaggg gacaaggatg tgtacagttt gcggctgcgg
     1561 tgacggcgaa gtgaaaatcg agggcggggc gcacgcccac cgccatgtcc atgccgacgg
     1621 cacggtgcat gaacacggcc acgcgcacga gtacgaacat gcgcacagcc acgatggcga
     1681 gattgccgac gcccacggcc acgcgcatac gcacgccgat ggcacgacgc atgtgcacga
     1741 acacgtccat cagggcgaac acgcccacga tcatggtcag caccaccatg gacatgatca
     1801 cgcgcatggc gaagcgcacc accacggcga cctcgactac
//
LOCUS       DAOSDX010000653         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21645_length_1835_cov_3.197753, whole genome shotgun sequence.
ACCESSION   DAOSDX010000653 DAOSDX010000000
VERSION     DAOSDX010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21645_length_1835_cov_3.197753"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..708
                     /locus_tag="PLN02_12700"
     CDS             118..708
                     /locus_tag="PLN02_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI53232.1"
                     /translation="MTSDLERQLDFLREIDRLKSIVRLSPLIDRSRRENSAEHSWHLA
                     LYALVLAEHAAGNVDVLRVVKMLLIHDIVEIDAGDVPFHLPATHVGQAERERLAAERI
                     FGLLPEPQASEFHDLWREFEAAESDDARFAKALDRFQPMLHNAATDGGTWVECGVTLE
                     QITTRCRPPIEGGAPALWEAAARMAGEHYRNRPDEA"
     gene            1068..>1835
                     /locus_tag="PLN02_12705"
     CDS             1068..>1835
                     /locus_tag="PLN02_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HOI53233.1"
                     /translation="MAFSQLYLYDHYGYAFLAVVGTFAMVWGIYWLMASSRWATRIRG
                     LHGVAPPFINIIGVLFGLTLAFLANDTWNAHDRATTAVYREADSLRSILTMARNIDSP
                     IMQQLNRQLIDYGEAIVAEWPQLAQRGVSSDAAAQADALLVLLIRPEIAQAAGGNGQM
                     LMLQKAADLRNDRDLRISLSQTHINPLKWLGMAFLGLLTLLSVAVVHAENAKAALVAT
                     LLFALAAAPTAAIVLVQGNPFQEPSIVSPAPIRHAIGE"
BASE COUNT          323 a          540 c          586 g          386 t
ORIGIN      
        1 ggcgaatcgc tcggacgcgt ccgggaagga cgcttcggac ccctgggcga agtggagaaa
       61 ataggccggg ttggctggtt tttcctgcaa gatttttagc gagatggcgg caaggtgatg
      121 acttccgatc tggaacggca gctggatttc ctcagggaaa tcgatcggct caagagcatc
      181 gtgcgactgt cgccgttgat cgaccgctcg cgccgcgaaa acagcgccga gcattcgtgg
      241 catctcgccc tgtacgcgct ggtgctcgcc gaacacgccg ccggcaacgt cgatgtgctg
      301 cgcgtcgtca agatgctgct gatccacgac atcgtcgaga tcgatgccgg tgacgtgcct
      361 ttccacctgc cggcgacgca tgtcggtcag gccgagcgcg aacgtctggc cgccgagcgt
      421 atcttcggtc tgctgccgga accccaggca agcgagtttc acgacttgtg gcgggagttc
      481 gaggcggcgg agagcgacga cgccagattc gccaaggccc tggatcgttt ccagccgatg
      541 ctgcacaacg cggcgaccga tggcggcacc tgggtggaat gcggggtgac gctagaacag
      601 atcacgacgc gctgtcgtcc gccgatcgag ggtggggcgc ctgcgctgtg ggaggccgcc
      661 gcccggatgg ccggggaaca ctatcgcaat cgtccggacg aggcttgagt tggctgccgg
      721 ggcgagtgga gatcgtttcg ccgacgaggc cgcctgcgtt caggcgccgc tgcaggtaat
      781 ccggatgcgt cgccaggccg cctgattgcc cggaaaatga gcggcgccgt gtttttcttt
      841 caagtcagtg gcttggctca tttctcccga tcttatccac agacttgcca aggctttctg
      901 gggattgtgc aacagtccgg aagtcggcct tcatgaagct catgtccatt cgatcaagca
      961 actgtccggt gttccggcgg cttgccggtc ggggttttcc tgcagcgacc ttgcgccggg
     1021 cctgttagag ttatggcgca tcagggcaaa tcgggcgctt atccagcatg gctttttcgc
     1081 agctttatct ctacgaccac tatggctatg cctttctcgc ggtggtcggc accttcgcaa
     1141 tggtgtgggg catttactgg ctgatggcgt cttcgcgatg ggccacccgg attcgtggct
     1201 tgcacggcgt cgcaccaccg ttcatcaaca tcattggggt tttgtttggc ctgaccctgg
     1261 ccttcctggc caacgacacc tggaatgcgc atgatcgggc aacgactgcc gtgtaccgtg
     1321 aagcggacag cttgcgcagt attctgacga tggcgagaaa tatcgattcg ccgatcatgc
     1381 agcagttgaa ccggcaactg atcgattacg gcgaggctat cgtcgccgag tggccacaac
     1441 tggcgcagcg cggtgtcagc agtgatgctg cggcgcaagc tgatgcactg ctcgtcctgt
     1501 tgatcaggcc cgaaattgcc caggcggcag gcggcaatgg gcaaatgctg atgctgcaaa
     1561 aggctgccga tttgcgtaac gaccgcgact tgcggatcag cctcagccag acccatatca
     1621 atccgctcaa gtggctgggc atggctttcc tcggtttgct gacgctgctc tcggtggctg
     1681 tcgtgcatgc cgaaaatgcc aaggcagcgc tcgtggcaac cctgcttttt gccctggcag
     1741 cagcgcccac ggcggcgatc gttctggtac agggcaaccc ctttcaggaa ccctcgatcg
     1801 tttcgccggc acccatacgc cacgccattg gcgag
//
LOCUS       DAOSDX010000654         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21688_length_1833_cov_2.550056, whole genome shotgun sequence.
ACCESSION   DAOSDX010000654 DAOSDX010000000
VERSION     DAOSDX010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21688_length_1833_cov_2.550056"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLN02_12710"
     CDS             <1..357
                     /locus_tag="PLN02_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOI53234.1"
                     /translation="RRINVVGARVLVLGLSFKENCPDLRNTKVVDIVRTLAGLNAVVD
                     VYDPWVDADEAQAEYGITPIGLPAAGQYDAVVIAVAHRQFAAQGVAAIRALCRPQAVI
                     YDVKHLFPREAVDGRL"
     gene            344..1084
                     /locus_tag="PLN02_12715"
     CDS             344..1084
                     /locus_tag="PLN02_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53235.1"
                     /translation="MADCDTPLNVVCLKWGTKYGPEYVNRLYRMVARHLRRPFRFHCL
                     TEYPQGLDPAVVVLPLDCQPGLSGWWYKLSLFAPEFHGLSGPMLYLDLDLVIVGNVDF
                     LAEQAGDFVILRNWSRNPMWNSSVMRFTVGAHPEVWQRFQADPDGVMASCNGDQEWIY
                     ECLPDAANWPAEKIVSYKKSLDSRAFPWLAKLGGERLGLRAPGWLDTPLPATAAIVVF
                     HGKPDPADVAWRAYGLWKRASFVARHWV"
     gene            1110..>1833
                     /locus_tag="PLN02_12720"
     CDS             1110..>1833
                     /locus_tag="PLN02_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53236.1"
                     /translation="MKQVLCMKWGQLYGPEYVNRLYAMVRANIEGPLRFVCLTDERAG
                     IRPEVECHDCPEIALPAPYNRRGWRKLTTYAASEQLFGFSGQWLFLDLDVVILGSLDD
                     FFSYQPEKSFIVMRNWTQPGRNIGNTSVYRFTVGADEYLLSRLLAEQDAILATFRNSQ
                     TYISRTVRELVFWPDAWCVLFKTHCVPPWPLRFWQTPKHPAEARVVAFPGVPNPHEAV
                     DGRWPAPAWKKSYKFIRPAPWIG"
BASE COUNT          293 a          632 c          593 g          315 t
ORIGIN      
        1 cggcgcatca acgtcgtcgg tgcgcgtgtc ctggtcctcg gcctcagctt caaggaaaac
       61 tgcccggacc tgcgcaacac caaggtggtc gatatcgtcc gtaccttggc cgggctcaac
      121 gccgtggtcg atgtctacga tccctgggtc gatgccgacg aggcgcaggc ggaatacggc
      181 atcacgccga tcggtctgcc ggccgccgga caatacgacg ccgtggtcat tgccgtcgcc
      241 caccgccagt tcgcggcgca gggcgtcgcc gcgatccgcg cgctgtgccg gccgcaggcg
      301 gtgatctacg acgtcaagca cctgtttccg cgcgaggccg tcgatggccg attgtgatac
      361 gccgctcaac gtcgtctgcc tgaaatgggg gacgaagtac gggccggaat acgtcaatcg
      421 cctgtaccgc atggtcgcgc gccacctgcg gcggccgttc cgctttcatt gcctgaccga
      481 atatccgcag gggctcgatc cggccgtcgt cgtgctgccg ctcgactgcc agccgggact
      541 gagcggctgg tggtacaagc tgtcgctgtt cgcgccggaa ttccacggcc tctccgggcc
      601 catgctctat ctcgacctcg acctggtgat cgtcggcaac gtcgatttcc tcgccgagca
      661 agcgggcgat ttcgtcatcc tgcgcaactg gtcgcgcaac ccgatgtgga acagctcggt
      721 gatgcgcttc acggtcggcg cccaccctga agtctggcag cgtttccagg ccgaccccga
      781 cggcgtcatg gcgagctgca acggcgacca ggagtggatt tacgaatgcc tgccggacgc
      841 cgccaactgg ccggcggaga agatcgtctc gtacaagaag agcctcgact cgcgcgcctt
      901 cccctggctc gccaagctgg gcggcgagcg gctcgggctg cgcgcgccgg gctggctcga
      961 cacgccgctg ccggcgacgg cggcgatcgt cgtcttccac ggcaagcccg atccggccga
     1021 cgtggcgtgg cgcgcctacg gtttgtggaa gcgggcgtcc ttcgtcgccc gtcactgggt
     1081 gtgagcccct ctcgaaaaag gcaggcaagg tgaaacaggt actgtgcatg aaatggggcc
     1141 agctctacgg cccggaatac gtcaaccggc tgtacgcgat ggtgcgcgcc aacattgagg
     1201 ggccactgcg cttcgtctgc ctgaccgacg agcgcgccgg catccggccc gaggtcgaat
     1261 gccacgactg cccggaaatc gccttgccgg cgccgtacaa ccggcgcggc tggcgcaagc
     1321 tgacgactta cgccgccagc gaacaactgt tcggtttcag cggccagtgg ctgttcctcg
     1381 acctcgacgt ggtgatcctc ggcagcctcg acgacttctt cagctaccag ccggagaaga
     1441 gctttatcgt catgcgcaac tggacgcagc ccgggcgcaa catcggcaac acctcggtct
     1501 accgcttcac cgtcggcgcc gacgaatacc tgctgagccg cctgctcgcc gagcaggacg
     1561 cgatcctggc gactttccgc aattcgcaga cctacatctc gcgcaccgtg cgcgaactgg
     1621 tcttctggcc cgacgcctgg tgcgtgctgt tcaagacgca ttgcgtgccg ccctggccgc
     1681 tgcgcttctg gcagacgccg aagcacccgg ccgaggcgcg ggtggtcgcc ttccccggcg
     1741 tgcccaaccc gcacgaggcg gtcgacggcc gctggccggc accggcctgg aagaagagct
     1801 acaagttcat ccgtccggcg ccgtggatcg gcg
//
LOCUS       DAOSDX010000655         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21726_length_1830_cov_3.520000, whole genome shotgun sequence.
ACCESSION   DAOSDX010000655 DAOSDX010000000
VERSION     DAOSDX010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21726_length_1830_cov_3.520000"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLN02_12725"
     CDS             <1..258
                     /locus_tag="PLN02_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53237.1"
                     /translation="PVNKVAEGRPHIVDMIKNNEISLIINTVEEKRGAINDSRSIRTS
                     GLQARVTMYTTIWGAEAAAEGIRNRGELVVYPIQALHAQLG"
     gene            326..802
                     /gene="greA"
                     /locus_tag="PLN02_12730"
     CDS             326..802
                     /gene="greA"
                     /locus_tag="PLN02_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOI53238.1"
                     /translation="MNKVPLTVNGAERLREELHRLKTVDRPWVIGAIAEARAHGDLSE
                     NAEYDAAKERQGFVEGRIQEIEGKLSNAQIIDPKLLDADGRCVFGATVEMEDQDSGDT
                     VTYQIVGEDEADIKTGKISVNSPVARALIGKYAGDIAQVQAPGGIREYEIIDIRYE"
     gene            805..1266
                     /locus_tag="PLN02_12735"
     CDS             805..1266
                     /locus_tag="PLN02_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HOI53239.1"
                     /translation="MRRLSEAFYQFAITLWVGGLWAIGYLAAPVLFAELGDRQLAGML
                     AGKLFALIAWFGLGAALYLLVFLFARSGARALRQAGFWLVVLMAAMAAVQLFGIQPLM
                     AQLKLDALPREVMESVFRDRFATWHGISSIVYLLQSLSGLVLVLWSGRGLR"
     gene            complement(1267..1614)
                     /gene="yhbY"
                     /locus_tag="PLN02_12740"
     CDS             complement(1267..1614)
                     /gene="yhbY"
                     /locus_tag="PLN02_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOI53240.1"
                     /translation="MQQLTSAQVRELRAQAHGLNPVVSISENGLSDAVLKEIDACLKA
                     HELIKIRVYGDSREDRLAYLERICAELSAAAVQHIGKLLVVYRENPEKLTAKDTAKPR
                     PAAPRRTKRSFQG"
     gene            1647..>1830
                     /locus_tag="PLN02_12745"
     CDS             1647..>1830
                     /locus_tag="PLN02_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI53241.1"
                     /translation="MKRTRTSKAWMREHVNDPYVQLAKKEGWRSRAAFKLMEIDDKDK
                     LLKRGEVVVDLGATPGG"
BASE COUNT          334 a          529 c          595 g          372 t
ORIGIN      
        1 ccggtgaaca aggtggccga aggccggccg cacatcgtcg acatgatcaa gaacaacgag
       61 atttcgctga tcatcaacac cgtcgaggaa aagcgcggcg cgatcaacga ttcgcgttcg
      121 atccgtactt ccggcctgca ggcgcgggtg acgatgtaca cgacgatctg gggtgccgaa
      181 gcggctgccg aaggcatccg caaccggggc gaactcgtgg tttatccgat ccaggcgctg
      241 cacgcgcaac tgggctgatt cactcgtacc accgccgggt cgcccacttt agggcgccgg
      301 cggtttgctt tttagcgagg cttacatgaa caaggttcca ctgaccgtca atggcgcgga
      361 gcgactgcgc gaagaactgc atcgcctgaa aaccgttgat cggccctggg tgatcggggc
      421 gattgccgaa gcgcgtgcgc atggcgacct ctccgaaaac gccgagtacg acgcggccaa
      481 ggagcgccag ggcttcgtcg aagggcgtat ccaggagatc gaaggcaagc tgtcgaatgc
      541 ccagatcatc gatcccaagc tgctcgatgc cgacggtcgc tgcgttttcg gcgccaccgt
      601 cgaaatggaa gaccaggatt ccggcgatac ggtgacttac cagatcgttg gcgaggacga
      661 ggcggacatc aagaccggca agatttcggt gaattcgccg gtggctcgcg cgttgatcgg
      721 caagtacgct ggcgatatcg cgcaggtgca ggcacccggt ggtatccgcg agtacgaaat
      781 catcgacatc cgttacgaat aagcatgcgt cgcctgtccg aagccttcta ccagtttgcg
      841 atcacgctat gggtcggtgg cttgtgggcg atcggctatc tggctgcccc ggtgttgttc
      901 gccgaactcg gcgatcgcca actggccggg atgctcgccg gcaagctgtt cgcgctgatt
      961 gcctggttcg gtcttggggc ggcgctctac ctgctggttt tcctgtttgc tcgctctggc
     1021 gcgcgtgcgc tccgccaggc cgggttctgg ctggtcgtgc tgatggcggc aatggcggca
     1081 gtgcagttgt tcggcatcca gccgctgatg gcgcagctca agctcgatgc cctgccgcgc
     1141 gaagtcatgg aaagcgtctt ccgcgaccgt ttcgcgacct ggcacggcat ttccagtatc
     1201 gtctatctgc tccagagctt gtccgggctc gttctggtcc tctggagcgg tcgcggcttg
     1261 cgttgatcag ccctgaaagc tgcgcttggt ccggcgcggt gccgccggac gcggttttgc
     1321 cgtatccttg gcagtgagct tctcggggtt ttcgcgatag acgacgagca gcttgccgat
     1381 gtgctggacc gccgcggcgg acagttcggc acaaatccgc tccaggtaag ccagtcgatc
     1441 ctcgcggctg tcgccgtaga cgcggatctt gattaattcg tgcgctttca ggcaggcgtc
     1501 gatttccttg agcaccgcgt cgctgaggcc gttttcggaa atcgagacga ccggattgag
     1561 gccgtgggcc tgggcacgca gttcgcggac ctgggctgaa gtcagttgtt gcattggaaa
     1621 acctttcaaa aacggcattt taccgaatga aacgaaccag aaccagcaag gcgtggatgc
     1681 gcgagcacgt caacgatcct tatgtccagc ttgccaagaa ggaaggctgg cgttcgcgcg
     1741 ccgccttcaa gctgatggag attgacgaca aggacaagtt gctcaagcgc ggcgaggtcg
     1801 tcgtcgacct gggggcgacg cccggcggct
//
LOCUS       DAOSDX010000656         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21765_length_1827_cov_2.493792, whole genome shotgun sequence.
ACCESSION   DAOSDX010000656 DAOSDX010000000
VERSION     DAOSDX010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21765_length_1827_cov_2.493792"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLN02_12750"
     CDS             <1..573
                     /locus_tag="PLN02_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOI53242.1"
                     /translation="RPRAPFIGQTAPTRVHLLLFVLRVDGLDSGLYLLPRGGAGDELA
                     TTLAARFPDATPVDDCPPHLGLRRLTRASLPELQRLARALNCHQDIAATSAFSLGMLA
                     DFTTADGAGYRELLREAGRVGQALYLEAEAAGVRGTGIGCFFDDPVHEVAGLGGERWQ
                     SVYHFTVGTPVIDERIESSPPYPQRQQEES"
     gene            570..1010
                     /locus_tag="PLN02_12755"
     CDS             570..1010
                     /locus_tag="PLN02_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOI53243.1"
                     /translation="MSPSLAQFLDHHGFPRDRLSAPQADGRFTPLMRACKEGRLDIVD
                     ELLTHGVDLAVSNIDGCNALWLACYNGNPGLVQRLIDAGIEIDRQNGNGATCLMYVSS
                     NGKADLVKLLLTNGADPALRNFDDFSALDLASTIDCLRLLKQAA"
     gene            complement(1011..1400)
                     /gene="crcB"
                     /locus_tag="PLN02_12760"
     CDS             complement(1011..1400)
                     /gene="crcB"
                     /locus_tag="PLN02_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOI53244.1"
                     /translation="MNAIGFAAVGLGAAFGAWSRWLLGLALNPLFVALPVGTLAANLI
                     GGYLIGVAVGVFHLNSGLPLALKLFAITGFLGGLTTFSTFSAEVVERLLAGQPALAIG
                     LAAIHLTGSLTATWLGLLSVGATRIWG"
     gene            1569..>1827
                     /locus_tag="PLN02_12765"
     CDS             1569..>1827
                     /locus_tag="PLN02_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOI53245.1"
                     /translation="MEFQDFADIEKATKRGRREFSRLGMGLLFVVCIMIYTALGIGIT
                     GPQGIMLVVASMIGGYMAMNIGANDVANNVGPAVGSHAITMT"
BASE COUNT          341 a          651 c          580 g          255 t
ORIGIN      
        1 cgcccgcgcg ccccgttcat cgggcagacg gcgccgacgc gggtccatct gctgctcttt
       61 gtgctgcgcg tggacggcct cgacagcggc ctctacctgt tgccgcgcgg cggcgccggc
      121 gacgaactgg cgacgacgct ggccgcccgt ttcccggacg cgaccccggt cgacgactgc
      181 ccgccgcacc tcggcctgcg ccgcctgacc cgggccagcc tgccggaact gcaacgcctg
      241 gcgcgcgccc tcaactgcca ccaggacatc gccgcgacca gcgccttctc gctcggcatg
      301 ctcgccgatt tcaccaccgc cgacggcgcc ggctaccgcg aactgctgcg cgaggccggg
      361 cgcgtcggcc aggcgctcta cctcgaagcc gaagccgccg gcgtgcgcgg caccggcatc
      421 ggctgcttct tcgacgatcc ggtacacgag gtcgccggac taggcggcga acgctggcag
      481 agcgtctatc acttcaccgt cggcacgccg gtgatcgacg agcgcatcga aagctcgccg
      541 ccctacccgc aacgacaaca ggaagaatca tgagcccaag cctcgcccaa ttcctcgacc
      601 accatggttt tccccgcgac cgcttatcgg cgccgcaggc cgacggccgc ttcacgccgc
      661 tgatgcgcgc ctgcaaggaa ggccgcctcg acatcgtcga cgaactgctg acgcacggcg
      721 tcgacctcgc ggtcagcaac atcgatggct gcaacgcgct ctggctcgcc tgctacaacg
      781 gcaatccggg actggtccag cggctgatcg acgccggcat cgagatcgac cgccagaacg
      841 gcaacggcgc cacctgcctg atgtatgtgt cctcgaacgg caaggccgat ctggtcaagt
      901 tgctgctgac caacggcgcc gacccggcgc tgcgcaactt cgacgatttc agcgcgctcg
      961 acctggcatc gacgatcgac tgcctgcggc tgctgaagca ggccgcctga tcagccccag
     1021 atccgcgtcg cgccgacgct gagcaggccg agccaggtcg ccgtcagcga gccggtgagg
     1081 tggatcgcgg cgagcccgat ggcgagcgcc ggttggccgg cgagcagacg ctcgacgact
     1141 tcggcggaaa aggtcgagaa ggtggtcaag ccaccgagga agccggtgat cgcgaacagc
     1201 ttgagcgcca gcggcaagcc ggagttgaga tgaaagacgc caacggcaac gccgatcagg
     1261 tagccgccga tcaggttggc ggcgagcgtg cccacgggca gcgcgacgaa cagcgggttg
     1321 agcgccaggc cgagcaacca gcgcgaccag gcaccaaaag cggcacccag tccaacggca
     1381 gcgaatccga ttgcgttcat accccctcct tttggcggat tctaccgcga gtcatccggc
     1441 atgccgcaac ggaagtccgc aacaaaaccg ccgccccaat gtcacaatag cgcctttcga
     1501 acggggagcg ccgacaaaag ctagaagcgg cgccagcagc atggcccagg aagggcacgg
     1561 gagaacgcat ggaatttcag gactttgccg acatcgagaa ggcaaccaag cgcggccgcc
     1621 gggagttcag ccggctcggc atgggcttgc tgttcgtcgt ctgcatcatg atctataccg
     1681 cgctcggcat cggcatcacc ggcccccagg gcatcatgct ggtggttgcc tcgatgatcg
     1741 gcggttacat ggcgatgaac atcggcgcca acgacgtcgc caacaacgtc ggcccggcag
     1801 tcggctcgca cgccatcacc atgaccg
//
LOCUS       DAOSDX010000657         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21767_length_1827_cov_2.448081, whole genome shotgun sequence.
ACCESSION   DAOSDX010000657 DAOSDX010000000
VERSION     DAOSDX010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21767_length_1827_cov_2.448081"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1348
                     /locus_tag="PLN02_12770"
     CDS             278..1348
                     /locus_tag="PLN02_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018606102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOI53246.1"
                     /translation="MSALFAPFTLRDLCLPNRIGMPPMCQYSACEGVANDWHVQHYGS
                     RAVGGVGLVIVEATAVLAEGRISPGDLGLWHDEQVEPLARVVRLIEGQGAVPAIQLAH
                     AGRKASVGLGWEAQRTLTPAEGGWTAVAPSPLAFGTYAAPLALDAGGIWRVVDAFAAA
                     ARRAVRAGFRAVEIHAAHGYLLHQFLSPLSNLRDDGYGGDFAGRTRLVREVVAAVRAV
                     LPAGMPLLIRLSATDWVEGGWDVEQTVELCRLLKEDGVDLVDVSTGGLVADAKIPAGP
                     GFQVPFATRIRSEAGLPVAAVGLITEAAQAEAIVANGEADLVLVGRELLRNPFWPLQA
                     AQALGAEVAWPKQYLRAKPVAR"
     gene            1362..1730
                     /locus_tag="PLN02_12775"
     CDS             1362..1730
                     /locus_tag="PLN02_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOI53247.1"
                     /translation="MSVVQEFRAWWRRRTAPARMLPGCAFPVLGGLQEGDIAQAVEIL
                     DDPRTPLRFVEQVLRAELGLARGDALLAAALAHGHGGVLIPVIDAATAATAASTLTEA
                     ARKAGWPLRCRAVALDGVRS"
BASE COUNT          236 a          527 c          714 g          350 t
ORIGIN      
        1 cttgccgtcc ttgctgacgg ccttcgattc gcagtccgcg gcttgcgcga aacagaggct
       61 tgcggtgaac aagccagcca gcaatcccat aagcagtttt ttcatcatga cctctcggta
      121 gttgatcgtt agtgcaatgc cgcgatggct tcctgccggc ggcggcttga gagtagcatc
      181 gttactgacg aaagcaatat gctgcggttt gggcttcctg ctggctgtgg caggattgcc
      241 cggcgctttc ccttttcatc cttgcccgga gattccgatg tccgccttgt tcgccccttt
      301 tactctgcgg gacctgtgct tgcccaaccg catcggcatg ccgccgatgt gccagtactc
      361 tgcctgcgaa ggtgtcgcca acgattggca tgtgcagcat tacggcagtc gggcggtggg
      421 cggggtgggg ctggtgatcg tcgaggcgac ggcggtgctg gcggaggggc ggatcagccc
      481 gggcgatctt ggcctctggc acgacgagca ggtcgagccg ctggcgcggg tggtgcgatt
      541 gatcgagggg cagggggcgg tgccggcgat ccagctggcg cacgccgggc gcaaggccag
      601 cgtcggtctg ggctgggagg cgcagcgcac gctgacgccg gcggaaggcg ggtggacggc
      661 ggtggcgcct tctcctttgg ccttcggcac ctatgcggcg ccgctggcgc tggatgcggg
      721 cggcatctgg cgtgtcgtcg atgcctttgc agcagcggcg cggcgggcgg tgcgggcggg
      781 tttccgggcg gtggagattc acgccgcgca cggctacctg ctgcatcagt tcctgtcgcc
      841 cttgtccaat ctgcgcgatg acggctacgg cggcgatttc gccggccgca cgcggctcgt
      901 gcgtgaggtc gtggcggcgg tgcgggcagt gttgccggcc ggcatgccgc tgctgatccg
      961 gctgtcggcg acagactggg tggagggcgg ctgggatgtg gagcagacgg tggaactttg
     1021 tcggctgctc aaggaggatg gggtcgatct ggtcgatgtg tcgaccggcg gcctggtggc
     1081 tgatgcgaag attcccgccg ggccgggctt ccaggtgccg ttcgcgacgc gcatccggag
     1141 cgaggctggc ttgccggtgg cggcggtggg cttgatcacc gaggcggcgc aggccgaagc
     1201 catcgtcgcc aatggcgagg ccgacctcgt gctggtcggc cgcgaactgt tgcgcaaccc
     1261 gttttggccg ctacaggcgg cgcaggccct gggggccgag gtggcctggc cgaagcagta
     1321 cctgcgggcg aaaccggtcg ctcgttgaga ggcttgcggt gatgtcggtc gtgcaggaat
     1381 tccgggcctg gtggcgacgc cggacggcgc ctgcgcggat gttgccgggc tgcgcctttc
     1441 cggtactggg cggcctgcag gaaggagaca tcgcacaggc ggtggagatc ctggacgatc
     1501 cccggacgcc gctgcgcttc gttgagcagg tgctgcgggc cgaactcggg ctggcgcgtg
     1561 gcgacgcgct gctggccgca gccttggcgc atggtcacgg tggggtgctg attccggtca
     1621 tcgatgcggc gacggcggcg acagcggcgt cgacgttgac ggaagcggca aggaaggcgg
     1681 gctggccgtt gcgttgtcga gctgtggcgc tggacggagt gcgttcatga agctgaccag
     1741 caaggcggtg tacaaattgt tgcgggcggc catttcgccc cgcttgccgg ccgggttcgt
     1801 ggcagcgccg ggcaatgttg ccgattg
//
LOCUS       DAOSDX010000658         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21778_length_1826_cov_3.189723, whole genome shotgun sequence.
ACCESSION   DAOSDX010000658 DAOSDX010000000
VERSION     DAOSDX010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21778_length_1826_cov_3.189723"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLN02_12780"
     CDS             <1..454
                     /locus_tag="PLN02_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOI53248.1"
                     /translation="ADINGLFVAVCRSIGIPARCVYGIRIGRSRLFPSLGLRSDDATR
                     AQHVRAEFYLPGYGWIPADPSDVRRAVAYEALQEGSGQLASLRRVLFGVWEMNWAGFH
                     SGNDLQLTGLSHTLPHFILPQLRVGAQNIDVLNPNNTSIRISARQIVL"
     gene            complement(522..>1826)
                     /gene="dnaB"
                     /locus_tag="PLN02_12785"
     CDS             complement(522..>1826)
                     /gene="dnaB"
                     /locus_tag="PLN02_12785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOI53249.1"
                     /translation="LLLDNNAWDRIGDQVVESDFYRDEHRRIFRQIRNLLEKAKPADV
                     VTVAEALDAAGENDQTGGLAYLGELAANTPSAANIRRYAEIVRERAVLRQLVATADEI
                     ASDALNPLGRDAASLLDEAESKIFKIAEAGAGHAEGFVHINPLLTQVVERIQELHDRD
                     NPSDITGVPTGFLDLDQKTSGFQPGDLIIVAGRPSMGKTAFALNIAENVAVDSGLPVG
                     VFSMEMGGAQLAMRMLASIGRLNSQSLRTGRMSDDEWSRLSFALGKLHEAPIYIDETG
                     GLSPANLRARARRLARQYGGKLGLLVIDYIQLMSGNKAGENRATEVSEISRSIKSLAK
                     ELQVPIVALSQLSRKVEERTDKRPMMSDLRESGAIEQDADVILMMYRDEYYKPDSPDK
                     GLAEVIIGKQRNGPTGTVRLAFLGEFTRFENLASGGFQDARD"
BASE COUNT          325 a          602 c          545 g          354 t
ORIGIN      
        1 cgccgacatc aacggcctgt tcgttgccgt ctgccgatca atcggcatcc cggcacgctg
       61 cgtctatggc atccggatcg gccgctcccg gctcttcccc tcgcttggcc tgcgcagcga
      121 cgatgccacg cgcgcccagc atgtccgggc cgagttctat ctgcccggct acggctggat
      181 tccggccgac cccagcgacg tccgccgggc ggtcgcctat gaagcgctcc aggagggcag
      241 cggccagctc gcatcgctgc gccgggtcct gtttggcgtc tgggaaatga actgggcggg
      301 attccattcc ggcaacgatc tgcaactgac tggcctgtcg cataccttgc cgcacttcat
      361 ccttccacaa cttcgagtcg gcgcacagaa tatcgacgtg ctcaacccga acaacacctc
      421 catccgcatc agcgcccgcc agatcgtgct gtaagagttc cgggcggcaa tttctccgcc
      481 ccgcccgacg cgaggttttg gctggcgccg aaacctcgaa cttagtcccg tgcgtcctga
      541 aagccgcccg acgcgaggtt ttcaaagcgg gtgaattcgc cgaggaaggc gaggcgcacg
      601 gtgccggtgg ggccgttacg ctgcttgccg atgatcactt cggccaggcc cttgtccggc
      661 gaatcgggct tgtagtattc gtcacggtac atcatcagga tcacgtcggc atcctgttcg
      721 atggcgccgg attcgcgcaa gtcggacatc atcggtcgct tgtcggtgcg ctcttcaacc
      781 ttccgtgaca gctgcgacag ggcgacgatc ggcacctgta gttccttggc cagcgatttg
      841 atcgagcgag agatttccga gacctcggtg gcgcggttct cgcctgcctt gttgccgctc
      901 atcagctgga tgtagtcgat caccagcagg ccgagcttgc cgccgtactg gcgcgccaga
      961 cgacgcgccc gggcacgcag gttggccgga ctgagaccac cggtctcgtc gatgtagatc
     1021 ggtgcttcgt gcagcttgcc gagggcaaac gacagacgcg accactcatc atcgctcatc
     1081 cgcccggtgc gcagactttg tgaattgagc cgaccgatcg aggccagcat ccgcatcgcc
     1141 agctgagcgc cgcccatttc catggaaaac acgccaaccg gaaggccgct atcgaccgca
     1201 acgttctcag cgatattgag cgcgaaagcc gttttcccca tcgacggacg accggcgacg
     1261 atgatcaggt cgcccggctg gaaaccggag gtcttctggt caagatcgag gaaacccgtc
     1321 ggcacgccgg tgatgtcgga aggattgtcg cgatcgtgca gttcctggat gcgctcgaca
     1381 acctgggtca gcagcggatt gatatgcacg aaaccttcgg cgtgaccagc gccggcttcg
     1441 gcgatcttga agattttcga ttcggcctcg tcgagcaggg aggccgcatc ccggcccagc
     1501 ggattcaacg catcggacgc aatttcgtcg gccgtcgcca ccagttgccg gagcaccgca
     1561 cgctcacgga cgatttccgc gtaacgccgg atattcgccg ctgacggcgt attcgccgcc
     1621 agctcgccaa gataggcaag tccaccagtc tggtcgtttt cgccagcggc atccagcgcc
     1681 tcggcgaccg tcaccacatc ggccggtttc gccttctcga gcaggttgcg gatttgccga
     1741 aaaatccgcc gatgctcgtc gcgatagaaa tcactctcga cgacctgatc gccgatgcgg
     1801 tcccaggcat tgttgtcgag cagcag
//
LOCUS       DAOSDX010000659         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21810_length_1824_cov_3.359525, whole genome shotgun sequence.
ACCESSION   DAOSDX010000659 DAOSDX010000000
VERSION     DAOSDX010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21810_length_1824_cov_3.359525"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLN02_12790"
     CDS             complement(<1..341)
                     /locus_tag="PLN02_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOI53250.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQISDAILDAILAQDKHSRVAAET
                     LCNTGLVVLAGEITTNANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSPD
                     IAQGVNAAYDD"
     gene            501..1340
                     /locus_tag="PLN02_12795"
     CDS             501..1340
                     /locus_tag="PLN02_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOI53251.1"
                     /translation="MVFLFRLLSRLPLSVIHRLGALLGQLTYLFSPSYRRHLRNNLAQ
                     AGIDPALRLPVAAETGKQMLELSRIWMRPLAEANAQVVEVVGRELLDAALQAGKGVVF
                     LTPHLGCFEITAQYLSSFGDITVLYRPPKSAAAQQLILMGRKRAQLHLAPADLSGVRA
                     LIKALKKGQMVGMLPDQAPKTGEGVWLDFFGRKAYTMTLAARLTETGAAVLMTWGERL
                     PGGRGYRVHFQALTAPLVGDTVARAQQINHEIETLIRQCPTQYLWGYNRYKTPRGAEP
                     PPA"
     gene            1435..>1824
                     /locus_tag="PLN02_12800"
     CDS             1435..>1824
                     /locus_tag="PLN02_12800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI53252.1"
                     /translation="MSSLNIEIRPITPPEVPAIASLAREIWQASYPGIITQEQIDFML
                     EQRYGHERLYDDLEDAHKWLDQAIHDGRRVGFAFSELYKGEFKLDKLYIHPDVQRRGV
                     GGQLIAHVAERARKQGYPCVILQVNKRN"
BASE COUNT          312 a          571 c          593 g          348 t
ORIGIN      
        1 ttgtcgtcgt aggcggcatt gacgccttgg gcgatgtccg ggctctgctt gtcgtaggcg
       61 acgagcacgg cgcagccctt gtagtcgatg ccgtattcgg tattgtcgta gccgatgcgc
      121 ttgatcgtgt cgcgcgccac ctggatgtaa tcgacgttgg cgttggtcgt gatttcgccg
      181 gcgaggacga ccaggccggt gttgcaaagg gtttcggcgg cgacgcggga atgcttgtcc
      241 tgagccagga tggcgtcgag gatggcgtcg gaaatctggt cggcgacctt gtcgggatgg
      301 ccttcggaaa ccgattccga ggtgaacagg tattcgctca tgggggtgct ccttggtcct
      361 tgtctccggc gttaccggct tcggaccacg agcaggcgac gctttagcgg attttgtgaa
      421 tcgccccgca agttgtctgt ttaactcggc gatcccataa tgataagcct ttgccgtccg
      481 ctttcaaccc ccggggcctc atggtttttc tgttccgtct gctctcgcgc ttgcccttga
      541 gcgtcattca ccggctcggc gccctgctcg ggcagctgac ttacctgttc tcgccaagtt
      601 accggcgcca cctgcgcaac aatctggcgc aggccggtat cgatccggcg ctacgcctgc
      661 cggtggcggc cgagaccggc aagcagatgc tggagctgtc gcgtatctgg atgcggccgc
      721 tggcagaggc caacgcccag gtcgtcgagg tggtcggtcg cgagctgctc gatgccgcgc
      781 tgcaggccgg caagggtgtc gttttcctga caccgcatct cggctgcttt gaaatcaccg
      841 cccagtacct ctcttccttt ggcgacatca ccgtccttta ccggccgccc aagtcggcgg
      901 cggcgcagca actgatcctg atggggcgca agcgggcgca gctgcatctg gcgccggccg
      961 atctgtccgg cgtgcgggcg ctgatcaagg cgctgaagaa gggacagatg gtcggcatgc
     1021 tgcccgatca ggcgccgaag accggcgagg gcgtgtggct ggatttcttc gggcgcaagg
     1081 cctacaccat gacgctggcc gcccgcctga ccgaaaccgg cgccgccgtg ctgatgacct
     1141 ggggcgagcg cctgcctggc gggcgcggct accgcgtgca cttccaggca ctgacggcgc
     1201 cgctcgtcgg tgatactgtg gcgcgggcgc aacagatcaa ccacgagatc gaaaccctga
     1261 tccgacaatg tccgacccag tacctgtggg gctacaaccg ctacaagacg ccgcgtggcg
     1321 ccgagccgcc gccggcctag aaactggcgg ggcaggtatc atcccgttgt tgtcggccag
     1381 ggccgcgccg gcggcaacct aaagaccatg gccaaccgag aacaggattt acccatgagc
     1441 agcctgaaca tcgagattcg ccccatcacg ccgccggaag tgccggcgat tgcctcgctg
     1501 gcgcgggaaa tctggcaggc gagctatccc gggatcatca cccaggagca gatcgacttc
     1561 atgctcgagc agcgttacgg tcacgagcgc ctttacgacg acctggaaga cgcccacaaa
     1621 tggctcgatc aggcgatcca cgacggacgg cgtgtcggtt tcgcattcag cgagctgtac
     1681 aagggcgagt tcaagctcga caagttgtac atccaccccg atgtgcaacg gcgcggcgtc
     1741 ggtgggcaac tgatcgccca cgtcgccgag cgggcgcgca agcagggtta cccctgcgtg
     1801 atcctgcagg tcaacaaacg caac
//
LOCUS       DAOSDX010000660         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_21919_length_1818_cov_2.273398, whole genome shotgun sequence.
ACCESSION   DAOSDX010000660 DAOSDX010000000
VERSION     DAOSDX010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21919_length_1818_cov_2.273398"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLN02_12805"
     CDS             complement(<1..296)
                     /locus_tag="PLN02_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOI53253.1"
                     /translation="MSLNRLKLTVAAIAVSAAIGGAWALGQAPVAAATPVAPPPTPVV
                     APLAAPVPAGYPDMRSIVAGNAPAVVNISVSGNRKTAANVPGMPQIDPDDPFFQ"
     gene            complement(464..>1818)
                     /locus_tag="PLN02_12810"
     CDS             complement(464..>1818)
                     /locus_tag="PLN02_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOI53254.1"
                     /translation="MAAEGHRPICQDTGIGMVFIKVGMHVTWPDATMSIQEMINEGVR
                     RAYANPDNPLRASVLAAPAGARKNTKDNTPAVVHFEIVEGHHVEVICAAKGGGSEAKS
                     KFAMLNPSDDLVDWVLHKIPEMGAGWCPPGIIGIGIGGTPEKAMLLAKESIMAPVDIH
                     ELKARGPQNRAEELRLELFEKINALGVGAQGLGGLTTVLDVKVLDYPCHAANLPVALV
                     PNCAATRHCHFHLDGSGPAKLEVPKLEDWPAVTWTPDLKSATRVNLDTLTKEEVASWK
                     VGQKLLLNGKMLTGRDAAHKRIADMLAAGEKLPVDFTNRIIYYVGPVDPVRDEVVGPA
                     GPTTATRMDKFTRMMLEKTGLISMVGKSERGPVAIEAIKDNKSAYLMAVGGAAYLVAK
                     AIKSAKVVGFADLGMEAIYEFDVQDMPVTVAVDSTGANVHESGPKEWQVKIGKIPVKS
                     "
BASE COUNT          268 a          558 c          640 g          352 t
ORIGIN      
        1 aactggaaga agggatcgtc ggggtcgatc tgcggcatgc cggggacgtt ggcggcggtc
       61 ttgcggttgc cgctgacgct gatgttgacg accgccggcg cgttgccggc gacgatgctg
      121 cgcatgtcgg gatagcctgc gggaacgggt gcggcgagcg gggcgacgac gggggtcggg
      181 ggcggcgcca ccggcgttgc tgcggcaacc ggtgcctggc ccagggccca ggcaccgcca
      241 atggcggcgg agacggcaat ggcggcgacg gtgagcttca ggcggttgag tgacatgcgg
      301 tcctcctcgg gtgatggtta cgggccgtat atttgcgctc gccagcgatt tttccatcag
      361 tccttgtgcg acgatttgtt acgaaattta tccggatcgt cctccaccaa gaaaaaaggc
      421 cgtccgcgca gcgcggacag cctttgccgg cggtggtgcc ggatcagctc ttgaccggaa
      481 tcttgccgat cttgacctgc cattccttgg ggccgctctc atgcacgttg gcgccggtcg
      541 aatcaacggc cactgtcacc ggcatgtcct gcacgtcgaa ctcgtagatg gcttccatgc
      601 cgagatcggc gaaaccgacc accttggccg acttgatcgc cttggcgacc aggtaggcag
      661 cgccgccgac ggccatcagg taggcactct tgttatcctt gatcgcctcg atggcgaccg
      721 gcccgcgctc ggacttgccg accatagaaa tgaggccggt cttttccagc atcatgcggg
      781 tgaacttgtc catgcgcgtg gcagtggtcg ggccggccgg gccgacgact tcgtcacgca
      841 ccgggtcgac cgggccgacg tagtagatga tgcggttggt gaagtccacc ggcagcttct
      901 cgccggccgc cagcatgtcg gcgatacgct tgtgcgcggc gtcgcggccg gtgagcatct
      961 tgccgttcag gagcagcttc tggccgacct tccaggaggc gacttcttcc ttggtcaggg
     1021 tgtcgagatt gacgcgggtc gccgacttca ggtccggcgt ccaggtgacg gccggccagt
     1081 cttccagctt cggcacttcc agcttggccg ggccgctgcc gtccaggtgg aagtggcagt
     1141 ggcgggtggc ggcgcaattc gggaccagcg cgaccggcag gttggcggcg tggcacgggt
     1201 aatccagcac cttgacgtcg agcacggtgg tgaggccacc gaggccttgg gcgccgacgc
     1261 cgagcgcgtt gatcttctcg aacagttcga gacgcagttc ctcggcgcgg ttctgcgggc
     1321 cgcgcgcctt cagttcatgg atgtcgaccg gcgccatgat cgattccttg gccagcagca
     1381 tggccttttc cggcgtgccg ccgatgccga tgccgatgat ccccggcggg caccagccgg
     1441 cgcccatttc cggaatcttg tgcagaaccc agtcgaccag gtcgtcggac gggttgagca
     1501 tggcgaactt ggacttggct tccgagccgc cgcccttggc cgcgcagatg acctcgacat
     1561 ggtggccttc gacgatctcg aagtgcacca cggccggggt gttgtccttg gtgttcttgc
     1621 gggcgccggc cggggcggcc agcacggaag cgcgcagcgg gttgtccggg ttggcgtagg
     1681 cgcggcggac gccctcgttg atcatttcct ggatgctcat cgtggcatcc ggccaggtga
     1741 cgtgcatgcc gaccttgatg aagaccatgc cgatgccggt gtcctggcag atcgggcgat
     1801 ggccttcggc ggccatgc
//
LOCUS       DAOSDX010000661         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22136_length_1802_cov_3.353749, whole genome shotgun sequence.
ACCESSION   DAOSDX010000661 DAOSDX010000000
VERSION     DAOSDX010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22136_length_1802_cov_3.353749"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLN02_12815"
     CDS             <1..358
                     /locus_tag="PLN02_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HOI53255.1"
                     /translation="PFWTEVTKTDGSTVPHLIVPYTLDTNDMRFCLPQGFSHGDPFFQ
                     YLKDSFDVLYAEGEESPKMLSIGMHCRLLGRPGRFLALQRFLDYIEGHDRVWVCRRLD
                     IARHWQATHPYRQDQK"
     gene            358..1386
                     /gene="alc"
                     /locus_tag="PLN02_12820"
     CDS             358..1386
                     /gene="alc"
                     /locus_tag="PLN02_12820"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HOI53256.1"
                     /translation="MIHAAPIVSAAADLPDWAKRSVNLADTRIGARALFATDEFFAPL
                     ERMLNPEPAVFVPGKYDANGKWMDGWETRRKRTTGYDWSLIKLGRRGILRGFDVDTSH
                     FTGNFAPAVSIDATDCESDDPEVLAKAHWTRILPSTTLSGNSHHLLAVDSADTWSHLR
                     INMYPDGGIARLRAYGQPVGVFDASEPDRIYDLVALENGGRAVAWNDAHFGSAANLIA
                     PDRGINMGDGWETRRRREPGNDWCILQLGAPGIIERVEVDTAHFKGNFPDRCSLQAAR
                     MDGGTDQSVITQSMFWPVLLGEQKLSMDAIHSFDGLADLGPVTHVRLNIIPDGGVSRL
                     RLWGKVAR"
     gene            1383..>1802
                     /locus_tag="PLN02_12825"
     CDS             1383..>1802
                     /locus_tag="PLN02_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018993097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HOI53257.1"
                     /translation="MKKLLPEPLTVAAFAPFGEVIAAAAATRHYAINGGNTERYHDLA
                     RLEPGADGRMIVSLFRGQPRALPFAIEMLERHPLGSQAFMPLGDQPYLIVVAPPGPPP
                     GPGDLRAFLAQPGEGVNYAPGTWHHPLLALNAVSDFLV"
BASE COUNT          300 a          701 c          538 g          263 t
ORIGIN      
        1 gccgttctgg accgaggtca cgaagaccga cggcagcacg gtgccgcacc tgatcgtgcc
       61 gtacacgctc gacaccaacg acatgcgctt ctgcctgccg cagggcttca gccacggcga
      121 cccgttcttc cagtacctga aggacagctt cgacgtgctc tacgccgaag gcgaggaaag
      181 cccgaagatg ctctccatcg gcatgcactg ccgcctgctc ggccggcccg ggcgtttcct
      241 cgcgctgcaa cgcttccttg attacatcga agggcacgac cgcgtctggg tctgccgtcg
      301 cctcgacatc gcccgccact ggcaggcgac ccatccctac cgccaggacc aaaaataatg
      361 atccacgccg cccccatcgt ttccgccgcc gccgacctgc ccgactgggc caagcgttcg
      421 gtcaaccttg ccgatacccg catcggcgcc cgcgcgctgt tcgccaccga cgagttcttc
      481 gccccgctcg aacgcatgct caaccccgag ccggcggttt tcgtgcccgg caagtacgac
      541 gccaacggca agtggatgga cggctgggaa acccgccgca agcgcaccac cggctacgac
      601 tggagcctga tcaagctcgg ccggcgcggc atcctgcgcg gctttgacgt cgataccagc
      661 cacttcaccg gcaacttcgc gccggccgtc tcgatcgacg ccaccgactg cgagtcggac
      721 gatcccgaag tgctggccaa ggcccactgg acgcgcatcc tgccctccac cacgctgagc
      781 ggcaacagcc accacctgct cgccgtcgat agcgccgaca cctggagcca tctgcgcatc
      841 aacatgtacc cggacggcgg catcgcccgt ctgcgcgcct acggccagcc ggtcggcgtc
      901 ttcgacgcca gcgagccgga ccgcatctac gacctcgtcg cgctggaaaa cggcggccgc
      961 gcggtggcct ggaacgacgc ccacttcggc tcggcagcca acctgatcgc tcccgaccgc
     1021 ggcatcaaca tgggcgacgg ctgggaaacc cgccgccgcc gcgaaccggg caacgactgg
     1081 tgcatcctgc aactgggcgc gcccggcatc atcgagcgcg tcgaagtcga caccgcccac
     1141 ttcaagggca acttccccga ccgctgctcg ctccaggcgg cgcgcatgga cggcggcacc
     1201 gaccagtcgg tgatcaccca gagcatgttc tggccggtgc tgctcggcga gcagaagcta
     1261 tcgatggacg ccatccacag cttcgacggc ctggccgacc tcggcccggt gacgcacgtg
     1321 cggctgaaca tcatcccgga cggcggcgtt tcaaggctgc gcctgtgggg caaggtggcg
     1381 cggtgaaaaa actgcttccc gaaccgctca ccgtggccgc cttcgccccc ttcggcgagg
     1441 tcatcgcggc ggccgcggcg acccggcatt acgcgatcaa cggcggcaac accgagcgct
     1501 accacgactt ggcccggctg gaacccggcg ccgacggacg gatgatcgtc agcctcttcc
     1561 gcggccagcc gcgcgccctg cccttcgcca tcgaaatgct ggaacgccat ccgctcggct
     1621 cgcaggcttt catgccgctc ggcgaccagc cctatctgat cgtcgtcgca ccgcccggcc
     1681 cgccgcccgg cccgggcgac ctgcgcgcct tcctcgccca gcccggcgag ggcgtgaact
     1741 acgcgcccgg cacctggcac cacccgctgc tggcgctgaa cgcggtcagc gacttcctcg
     1801 tc
//
LOCUS       DAOSDX010000662         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22193_length_1799_cov_2.725344, whole genome shotgun sequence.
ACCESSION   DAOSDX010000662 DAOSDX010000000
VERSION     DAOSDX010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22193_length_1799_cov_2.725344"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="PLN02_12830"
     CDS             <1..1690
                     /locus_tag="PLN02_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI53258.1"
                     /translation="AVVLSGIVALTLTPALCAILLKAQHTESKLFAPVNRLFERLTNN
                     YTRTVSFTLKHGIVGSLVFVGVIAVTGFFFRIIPGSFVPAEDQGYLISALMLPDAATL
                     KRTQDTGERMRSMIAKDEAVKHTFVVSGFDLIGGGNKPNAGTMFIPLKDWSERQGKAQ
                     DLAGKFMGIGMMQPDGMGLVFNPPPIMGLGTAGGFEVYVQNRVDGDAKKLHEVVQSFM
                     GELQKHPEFTRISTFFRPTVPQIFVEVDEPKALALGIPLADVYSTLQSTMGALYVNDF
                     NKAGRVYRVQLQAEANYRMKPDDIGKVYVKSTSSGKMIPLSAISTVKSVIGPEQVERF
                     NGFVAAKVMGDSKPGISSGDAIRIVEEVAEATLPEGYAISWTGQAFQEKQTSGSSLQA
                     FGFAIIMVFLILAAQYEKWSLPVAVVMAVPFALMGALTAIWLRGMPNDIYFQIGLVVL
                     IGLASKNAILIVEFAAQKYAEGMSVAEAAVEAARLRLRPIIMTSLAFVLGVFPLVKAT
                     GAGAAARQSMGTGVFGGMIAATFIATIFIPLFFKWLERGKQIDPRHLDVEEEKA"
BASE COUNT          307 a          611 c          556 g          325 t
ORIGIN      
        1 cgccgtggtg ctctccggca tcgttgcgct gaccctgacg ccggccctgt gcgccatcct
       61 gctcaaggcg caacacaccg agagcaagct gttcgcgccg gtcaaccgcc tgttcgagcg
      121 cctcaccaac aactacaccc gcaccgtcag cttcacgctc aagcacggca tcgtcggctc
      181 gctcgtcttc gtcggggtga ttgccgtcac cggcttcttc ttccgcatca tcccgggcag
      241 cttcgtgccg gccgaagacc agggctacct gatctcggcg ctgatgctgc cggatgcggc
      301 gacgctcaag cggacccagg ataccggcga gcggatgcgc tcgatgatcg ccaaggacga
      361 agcggtcaag cacaccttcg tcgtttccgg cttcgacctg atcggcggcg gcaacaagcc
      421 gaacgccggt accatgttca ttccgctcaa ggactggtcg gaacgccagg gcaaggcgca
      481 ggacctggcc ggcaagttca tgggcatcgg catgatgcag ccggacggca tgggtctggt
      541 cttcaacccg ccgccgatca tgggcctcgg caccgccggc ggcttcgagg tctatgtcca
      601 gaaccgcgtg gacggcgacg ccaagaagct gcacgaagtg gtccagagct tcatgggcga
      661 gttgcagaag catccggaat tcacccgtat ctcgactttc ttccggccga ccgtgccgca
      721 gatcttcgtc gaggtcgacg agccgaaggc gctcgccctg ggcattccgc tggccgacgt
      781 ctattcgacg ctgcaaagca cgatgggcgc cctctacgtc aatgacttca acaaggccgg
      841 ccgcgtctat cgcgtgcagt tgcaggccga ggccaactac cggatgaagc cggacgacat
      901 cggcaaggtc tacgtgaaga gcacgagcag cggcaagatg attccgctgt cggcgatcag
      961 caccgtcaag agcgtgatcg gtccggaaca ggtcgagcgc ttcaacggtt tcgtcgccgc
     1021 caaggtgatg ggcgattcga agcccggcat cagctccggc gacgccatcc gcatcgtcga
     1081 ggaagtggcc gaggcaacat tgcccgaggg ctacgcgatt tcctggaccg gccaggcctt
     1141 ccaggaaaag cagacctccg gctcgtcgct gcaggctttc ggcttcgcca tcatcatggt
     1201 cttcctgatc ctcgccgccc agtacgaaaa gtggtcgctg ccggtggccg tcgtgatggc
     1261 tgtgccgttc gcgctgatgg gcgcgctgac ggcaatctgg ctgcgcggca tgccgaacga
     1321 catctacttc cagatcggtc tggtggtgct gatcggcctc gcctcgaaga acgccatcct
     1381 gatcgtcgag ttcgccgccc agaagtacgc cgagggcatg agcgttgccg aagcggcggt
     1441 cgaggccgcc cgcctgcgcc tgcggccgat catcatgacc tcgctcgcct tcgtgctcgg
     1501 cgtcttcccg ctggtcaagg ccaccggcgc cggcgctgcc gcccgccagt cgatgggtac
     1561 cggcgtcttc ggcggcatga tcgcggcgac cttcatcgcc accattttca tcccgctctt
     1621 cttcaaatgg ctggagcgcg gcaaacagat cgatccgagg catctcgacg tcgaagagga
     1681 gaaagcatga tgtcccgctc cgtattccgc ctgaccccgc tggcgctggc cgtgctgctc
     1741 tccggctgcg ccgtcggccc ggactacctg cgcccggcca actggctgcc ggccgcctt
//
LOCUS       DAOSDX010000663         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22208_length_1798_cov_3.390706, whole genome shotgun sequence.
ACCESSION   DAOSDX010000663 DAOSDX010000000
VERSION     DAOSDX010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22208_length_1798_cov_3.390706"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLN02_12835"
     CDS             complement(<1..458)
                     /locus_tag="PLN02_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOI53259.1"
                     /translation="MHLLPFADLFLALCLGAALGFVGGLFGIGGGIVAIPLFVLAFAM
                     DQATAQGTALVLMVPNLLIGWWRYSQKHPVGWRAALAIAAPATLTTWALARFATRLDG
                     HLLGQMFSVFLGLLALRLLWQGRGGRQPVSGAPLRAAALPLVGVAGGSSMG"
     gene            556..1476
                     /locus_tag="PLN02_12840"
     CDS             556..1476
                     /locus_tag="PLN02_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI53260.1"
                     /translation="MTDIPDLAFFSLLARQPSLAAAAQALGVTPPAVSRRLAALERRL
                     GVRLLNRTTRRLSLTPEGERYLEDGEAILRDIERLERSLSASREQPQGLLRIGAGFGF
                     GRRHLGPAISDFVRDWPDVEVILHLADRALDLTEHALDIGIRFGPPPDARILARRIAP
                     NRRLLCAAPAYLAARGTPRTPRELAAHDCIVIREHERAYNNWQLTDGSTQVTVKVRSP
                     LAVNHGEIAVDWALAGHGIVLRSEWDIAADLRSGRLVRVLPEWAGSAADIHAVYPQRH
                     LLSAKVRVFLDFLERRFAGYSVDSQNPGPW"
     gene            1531..>1798
                     /locus_tag="PLN02_12845"
     CDS             1531..>1798
                     /locus_tag="PLN02_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53261.1"
                     /translation="MPRLSLAPLRRPLILVPLLLLFGLLAVFLLRPLFGSAVDVLVVR
                     QGEMRQSVVASGRVRSPQRSELAAQVSAQVRAVHVVEGQGVAAGD"
BASE COUNT          277 a          684 c          578 g          259 t
ORIGIN      
        1 cccatgctgc tgccaccggc aacgccgacc agcggcaacg cggctgcccg cagcggcgcc
       61 ccgctgaccg gctggcggcc accccgcccc tgccacagca ggcgcagcgc aagcaggccg
      121 aggaagacac tgaacatctg accgagcaag tgcccgtcga gacgtgtcgc aaagcgcgcc
      181 aaggcccagg tggtcagcgt tgccggtgcg gcgatggcga gcgccgcgcg ccagccgacc
      241 gggtgcttct ggctgtaacg ccaccagccg atcagcagat tgggcaccat cagcaccagc
      301 gccgtcccct gcgcggtcgc ctggtccatc gcgaaagcca gcacgaacag cggaatggcg
      361 acgatgccgc cgccgatgcc gaacaggccg ccgacgaagc cgagcgcggc gccgagacag
      421 agggcgagga agaggtcggc gaacggtagc aggtgcattt ggggcaggag cgggtgacgg
      481 gacgacccat gatattcatc cacatcatcg atacaatggc gcatcgatca attgattatt
      541 gcgtacaggt aacgaatgac cgacattccc gacctcgcct tcttcagcct gctcgcccgc
      601 cagcccagcc tggcggcggc ggcgcaggcg ctcggcgtga cgccgccggc ggtcagtcgc
      661 cgcctggcgg cgctggaacg ccggcttggc gtgcgcctgc tcaaccggac gacgcggcgc
      721 ctgagcctga cgccggaagg cgaacgctac ctggaggacg gcgaagccat cctgcgcgac
      781 atcgaacgcc tcgagcgctc gctttcggcc agccgcgaac agccgcaggg cctgctgcgc
      841 atcggcgccg gcttcggctt cggccggcgc catctcggtc cggcgatctc cgatttcgtc
      901 cgcgactggc cggacgtcga ggtcatcctc catctcgccg accgcgccct cgacctgacc
      961 gagcacgcgc tcgacatcgg catccgcttc gggccgccgc ccgacgcccg catcctggcc
     1021 cgccgcatcg cccccaaccg ccgcctgctc tgcgccgccc cggcctatct cgccgcccgc
     1081 ggcacgccgc ggaccccgcg cgagctggcc gcacacgact gcatcgtcat ccgcgaacac
     1141 gagcgcgcct acaacaactg gcaactgacc gacggcagca cgcaggtgac ggtcaaggtg
     1201 cgcagcccgc ttgccgtcaa ccacggcgag atcgccgtcg actgggcact ggccgggcac
     1261 ggcatcgtcc tccgatcgga atgggacatc gccgccgacc tgcgcagcgg ccgcctggtt
     1321 cgcgtcctgc cggaatgggc gggcagcgcc gccgacatcc acgccgtcta tccgcaacgc
     1381 cacctgctgt cggccaaggt gcgcgtcttc ctcgactttc tcgaacggcg tttcgccggc
     1441 tacagtgtcg atagccaaaa ccccggccca tggtgagccg tttgcggtta ccatgcgcga
     1501 ctgccatatt agctttccct gattccggcc atgccccgcc tttcccttgc ccccttgcgc
     1561 cgcccgctga tcctcgtgcc gctgttgctg ctgttcgggc tgctcgccgt cttcctgctg
     1621 cgcccgctgt tcggcagcgc ggtcgacgtg ctggtcgtcc gccagggcga gatgcgccag
     1681 tctgtggtcg ccagcggccg cgtccgctcg ccgcagcgca gcgaactggc cgcccaggtc
     1741 agcgcgcaag tgcgcgccgt gcatgtcgtc gaaggccagg gcgtcgccgc cggcgacc
//
LOCUS       DAOSDX010000664         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22217_length_1798_cov_2.642570, whole genome shotgun sequence.
ACCESSION   DAOSDX010000664 DAOSDX010000000
VERSION     DAOSDX010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22217_length_1798_cov_2.642570"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /gene="tldD"
                     /locus_tag="PLN02_12850"
     CDS             complement(<1..1423)
                     /gene="tldD"
                     /locus_tag="PLN02_12850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HOI53262.1"
                     /translation="MSQPNALAQAESLLLTPFSLSEADLSRTFGQILTHQVDYADLYF
                     QYSRSEAWSLDEGIVKSGSFNIDQGVGVRAISGEKTAFAYSDDISAAALGDAASAVRA
                     IAAAGQSGALPALRASAGGRALYLPNDPLASLPAEAKVKLLERLEGFARALDPRVLQV
                     NASLAGEYEVVLVAGSDGRLAADIRPLVRCSISVIVEENGKREQGASGGGGRFDFGYF
                     DDSVLQRYAQEAVHQAVTNLKAEPAPAGQMTVVLGAGWPGILLHEAVGHGLEGDFNRK
                     GSSTFAGRIGERVAAKGVTVIDDGTIADRRGSLNIDDEGNPSQRTVLIEDGILKGYMQ
                     DSLNARLMGVAPTGNGRRESFAHLPLPRMTNTMMLAGSQDPQEIIASVKKGIYAANFG
                     GGQVDITSGKFVFSMSEAYLIEDGKVTRPIKGATLIGNGPDAMTRVSMIGNDLKLDPG
                     VGKCGKEGQSVPVGLGPPTPPLDG"
     gene            complement(1433..>1798)
                     /locus_tag="PLN02_12855"
     CDS             complement(1433..>1798)
                     /locus_tag="PLN02_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOI53263.1"
                     /translation="PFGRIALSICYDLRFPELYRSLAPVDLILVPAAFTETTGRAHWE
                     ILLRARAIENQCYLLAVGQGGRHESGRLTHGNSMIVDPWGEILDRKLKGPGVVIADLD
                     HQRIAEVRESLPALAHRKL"
BASE COUNT          269 a          580 c          646 g          303 t
ORIGIN      
        1 gtccatcaag cggcggcgtc ggcggaccaa ggccgaccgg cacgctctgc ccttccttgc
       61 cgcatttgcc gacgccggga tcgagcttga ggtcgttgcc gatcatcgag acgcgggtca
      121 tcgcgtccgg cccgttgccg atcagcgtcg cccccttgat cggccgcgtc accttgccgt
      181 cttcgatcag ataggcctcg ctcatcgaga agacgaactt gccgctggta atgtcgacct
      241 ggccgccgcc gaagttggcg gcgtagatgc ctttcttcac cgaagcgatg atttcctgcg
      301 gatcctggct gccggccagc atcatggtgt tggtcatgcg cggcagcggc aggtgggcga
      361 aggattcgcg gcgcccgttg ccggtcggcg cgacgcccat caggcgggcg ttcaggctgt
      421 cctgcatgta gcccttgagg atgccgtcct cgatcagcac ggtgcgctgc gacggattgc
      481 cctcgtcgtc gatgttgagc gaaccgcgcc ggtcggcgat ggtgccgtcg tcgatcacgg
      541 tgacgccctt ggcggcgacg cgctcgccga tgcgaccggc gaaggtcgag ctgcccttgc
      601 ggttgaaatc gccttccagg ccgtggccga ccgcttcgtg caacaggatg ccgggccagc
      661 cggcgccgag gacgacggtc atctggccgg ccggcgccgg ctcggccttg aggttggtca
      721 ccgcctgatg caccgcctcc tgggcgtagc gttgcagcac gctgtcgtcg aaatagccga
      781 agtcgaaacg accgccgccg cccgaggcgc cttgctcgcg cttgccgttt tcctcgacga
      841 tgaccgagat cgaacagcgc accagcggcc ggatgtcggc ggccagccgg ccgtcggaac
      901 cggcgaccag gaccacctcg tactcgccgg cgagcgaggc attgacctgc agcacgcgcg
      961 ggtcgagcgc ccgggcaaag ccctccaggc gttcaagcaa tttgaccttg gcctcggccg
     1021 gcagcgaagc gagcgggtcg ttcggcaggt agagggcacg cccgccggcc gacgcgcgca
     1081 gcgccggcaa cgcgccggac tggccggcgg cggcgatggc gcgcacggcg ctggcggcgt
     1141 cgccgagcgc cgcagcactg atgtcgtcgg agtaggcgaa ggcggtcttc tccccggaga
     1201 tggcgcgcac gccgacgccc tggtcgatgt tgaagctgcc cgacttgacg atgccttcgt
     1261 cgaggctcca ggcctcggag cgcgaatact ggaagtagag gtcggcgtaa tcgacctggt
     1321 gcgtcagtat ctgcccgaag gtccgcgaca ggtcggcctc gctcagggag aacggcgtca
     1381 gcagcaggga ttcggcttgc gccagcgcgt tgggttggct catggaaggt ccttatagtt
     1441 tgcggtgggc gagcgccggc aggctctcgc ggacttcggc gatgcgttgg tggtcgaggt
     1501 cggcgatgac gacgccgggc cccttcagct tgcggtcgag aatttcgccc cagggatcga
     1561 cgatcatgct gttgccgtgg gtcaggcggc cgctttcgtg gcgtccgccc tggccgactg
     1621 ccagcagata gcactggttc tcgatggcgc gggcgcggag caggatttcc cagtgggcgc
     1681 ggccagtcgt ctcggtgaag gccgccggca ccaggatcag gtcgaccggg gcgaggctac
     1741 ggtaaagctc gggaaagcgc aggtcgtagc agatcgacag ggcgatgcgg ccgaacgg
//
LOCUS       DAOSDX010000665         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22226_length_1797_cov_2.652124, whole genome shotgun sequence.
ACCESSION   DAOSDX010000665 DAOSDX010000000
VERSION     DAOSDX010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22226_length_1797_cov_2.652124"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLN02_12860"
     CDS             complement(<1..345)
                     /locus_tag="PLN02_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HOI53264.1"
                     /translation="MPNPIKRTVFFISDGTGLTAEALGHSLLTQFEDVEFRQIRVPFL
                     DSIDKAQETLQRINAQGEADGMRPIVFTTLVNQDLATIIHQADAFCLSYFETFLAPLE
                     VELGRKSSHTVGR"
     gene            complement(360..>1797)
                     /locus_tag="PLN02_12865"
     CDS             complement(360..>1797)
                     /locus_tag="PLN02_12865"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOI53265.1"
                     /translation="LSENERIDLLCAEIASPRPLYSPWLNYDEESRGELAIFFAAREL
                     REKYGDAALPNSIISKTDGVSDLLELALLLKEAGLLRPGPEPALDVNIIPLFETIEDL
                     QKSAAIMDWLFALPAYRRLVSARGDEHEVMLGYSDSNKDGGFLTSGWELYKAEIQLAE
                     TFAEHGIRLRLFHGRGGSVGRGGGPSYHAILAQPAGAVSGQIRLTEQGEVISTKYGNP
                     DTGRRNLEVLLAATLEASLTDHENRVEPASQFHGVMDELSQRAFNAYRGLVYETPGFT
                     TYFRQSTVVSEIASLNIGSRPASRKASERIEDLRAIPWVFSWAQCRLMLPGWYGFGSA
                     VDGWLAENPDGLATLRRMLKSWPFFQSLLSNMDMVLAKTDLAIASRYAELVADGELRE
                     RIFGRIVAEWQLTRRHLLAILEQDDFLADNPMLKRSLQLRAPYMDPLNHLQVELLKRH
                     RAGDGDERVARGIHITINGIAAGLRNSG"
BASE COUNT          278 a          501 c          687 g          331 t
ORIGIN      
        1 gcggccgacg gtgtggctgg acttgcggcc gagttcgacc tccagcgggg cgaggaaggt
       61 ttcgaagtag gacaggcaga aggcgtcggc ctggtggatg atcgtcgcca ggtcctggtt
      121 caccagcgtg gtgaagacga tcggccgcat gccgtcggct tcgccttgcg cgttgatgcg
      181 ctgcagcgtt tcctgggctt tgtcgatgct gtccaggaag gggacgcgga tttgccggaa
      241 ttcgacgtcc tcgaactggg tcagcaggct gtggccgagg gcctcggcgg tgaggccggt
      301 gccgtcggag atgaagaata cggtgcgttt gatcggattg ggcatgatgg cgggggattc
      361 tagccgctgt tgcgcaggcc ggcggcgatg ccgttgatgg tgatgtggat gccgcgggcg
      421 acgcgctcgt cgccgtcgcc ggctcggtgg cgcttgagca gttcgacctg caggtggttg
      481 agcgggtcca tgtagggtgc gcgcagctgc agcgagcgct tgagcatcgg gttgtcggcg
      541 aggaaatcgt cctgttcgag gatggccagc aggtggcggc gggtcagttg ccattcggcg
      601 acgatgcggc cgaagatgcg ttcgcgcagt tcgccatcgg cgaccagctc ggcatagcgc
      661 gaggcgatgg cgaggtcggt cttggccagc accatgtcca tgttcgagag caggctctgg
      721 aagaagggcc aggacttgag catgcggcgc agcgtggcga ggccgtcggg gttttccgcc
      781 agccagccgt cgacggcgct gccgaagccg taccagccgg gcagcatcag ccggcattgc
      841 gcccaggaga agacccaggg aatcgcccgc aggtcctcga tgcgctcgga agccttgcgc
      901 gatgccgggc ggctgccgat attgagcgag gcgatttcgg aaaccaccgt cgattgccgg
      961 aagtaggtgg tgaagcccgg ggtttcatag accaggccgc ggtaggcatt gaaggcgcgc
     1021 tgcgacagtt cgtccatcac gccgtgaaac tggctggccg gttcgacgcg gttctcatgg
     1081 tcggtgaggc tggcttccag ggtggcggcg agcagcactt cgaggttgcg gcggccggtg
     1141 tcggggttgc cgtacttggt cgagatgact tcgccctgtt cggtcaggcg gatctggccg
     1201 gaaacggcgc cggccggctg ggccaggatg gcgtggtagg acggaccgcc gccgcggccg
     1261 accgagccgc cgcggccatg gaacaggcgc aggcggatac cgtgttcggc aaaggtttcg
     1321 gcgagctgga tttcggcctt gtaaagctcc cagcccgagg tcaggaagcc gccgtccttg
     1381 ttgctgtccg agtagccgag catgacctcg tgctcgtcgc cgcgggcgct gaccaggcgg
     1441 cggtaggcgg gcagcgcgaa cagccagtcc atgatggcgg cgctcttctg caagtcctcg
     1501 atggtttcga agagcgggat gatgttgacg tcgagcgccg gttccgggcc cggccgcagc
     1561 agtccggctt ccttgagcag cagcgcgagt tcgagcaggt cggagacgcc gtcggtcttc
     1621 gagatgatgc tgttgggtag ggccgcgtcg ccgtatttct cgcgcagttc gcgggcggca
     1681 aagaagatcg ccagttcgcc gcggctttcc tcgtcgtagt tcagccaggg agaatagagc
     1741 gggcgcggcg aggcgatctc ggcgcacagc aggtcgatcc gttcgttttc ggaaagc
//
LOCUS       DAOSDX010000666         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22229_length_1797_cov_2.384615, whole genome shotgun sequence.
ACCESSION   DAOSDX010000666 DAOSDX010000000
VERSION     DAOSDX010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22229_length_1797_cov_2.384615"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLN02_12870"
     CDS             complement(<1..367)
                     /locus_tag="PLN02_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53266.1"
                     /translation="MRNHIAHRAPLATPVFIAPYLSPAVRQLCVENGVSFLDLEGNAR
                     IAFGGVFIERQVTNKPVAAQRELKSLFRPKSAQVLRAMLREPGRAWRVTELSEIAGVS
                     LGHVSNVRTGLIDREWARAS"
     gene            389..799
                     /locus_tag="PLN02_12875"
     CDS             389..799
                     /locus_tag="PLN02_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53267.1"
                     /translation="MPRLPVRFDLADELVRLSVHQQPDDPVGFPVAGCEAGIDAFNVK
                     DRNLFKQAAQRFMGAGFFGFQVFGGFQHLLKIARPVKFWEISFLEVVSAIVEIIIELE
                     MPGCRRGIVRQGAALRQGLQGAGRRFHQEAFQMR"
     gene            complement(810..1565)
                     /locus_tag="PLN02_12880"
     CDS             complement(810..1565)
                     /locus_tag="PLN02_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOI53268.1"
                     /translation="MRPAADPPPSRPFAFGFWLALPLAAMGLLLLVDPAPLDFWITGH
                     FHVPGSGFVARDNYWLETVLHDRAKTLVIVFVLALGVALAASARLAALRSWRGPLAYA
                     LLAITLSTAVVPPLKVVSAVQCPWDLSDFGGSETFSPLLAPRPPTEHPGRCWPGGHAA
                     TGFSLIALFFALRDRRPRLARGLLAGALAFGSLLAAGRMMQGAHFLSHNLWTLLLDWS
                     ICLLVYRFTLYRPARLPSAAHRRRAIGIPAGTS"
     gene            complement(1549..>1797)
                     /locus_tag="PLN02_12885"
     CDS             complement(1549..>1797)
                     /locus_tag="PLN02_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53269.1"
                     /translation="AARFAYYIHLGLFDGKDLAILSPRRGMRRHDDALGESQESRSNA
                     DDPLLRRDIAYYQGASHAFRNGLLAWRPLSTAGDATGR"
BASE COUNT          323 a          539 c          650 g          285 t
ORIGIN      
        1 ccgaagcgcg cgcccattcg cgatcgatca ggccggtacg cacattgctg acgtgcccga
       61 ggctgacccc ggcgatttcc gacaactcgg tgacccgcca cgcgcggccg ggttcgcgca
      121 gcatggcgcg cagcacctgg gcggatttcg gccggaacag cgacttcagt tcgcgctgcg
      181 cggccaccgg cttgttcgtc acctgacgct cgatgaaaac gccgccaaag gcaatccggg
      241 cgttgccttc gaggtcgagg aagctgacgc cgttctctac gcacaactgc ctcaccgccg
      301 gtgagaggta gggggcgatg aaaacgggcg ttgccagggg cgcccggtgc gcgatgtggt
      361 tccgcaactc cagcagtgcg gagcgggcat gccgcggttg cccgttcgat ttgacctcgc
      421 agacgagctg gtgcgcttgt ccgtccacca gcagccggac gatccagtcg ggttcccggt
      481 cgccggatgc gaggccggca tcgatgcctt caacgtgaag gatcggaatc ttttcaagca
      541 agccgcgcag cgcttcatgg gcgcgggctt cttcgggttt caggtttttg gcggatttca
      601 gcatttgctg aaaatagcac ggccagtgaa attttgggaa atttcattcc tggaggtggt
      661 ttcagcgatt gttgaaatca tcatcgaact tgaaatgccc ggatgccgta ggggcatcgt
      721 ccgtcagggg gcagccttgc gccaggggct gcaaggtgca ggccggcgct ttcatcagga
      781 ggcgtttcag atgcgctgaa ccagccccct cagcttgttc ccgccggtat gccgattgcc
      841 cgtcgcctgt gcgctgcgct cggcaatcgg gccgggcggt agagggtgaa gcgatagacg
      901 agcaggcaga tgctccagtc gagcagcagc gtccacaggt tgtgcgacag gaaatgggcg
      961 ccctgcatca tgcgcccggc agcaagcaga ctgccgaagg cgagggcacc ggccagcagg
     1021 cctcgggcca ggcgcggtcg gcggtcgcgc agggcgaaga acagtgcgat cagggaaaaa
     1081 cccgtggccg cgtgaccacc cggccagcag cgtccgggat gctcggttgg cggtcggggc
     1141 gccaacagcg gtgaaaaagt ctcgctgccg ccaaagtcgc tgaggtccca aggacactgc
     1201 acggcgctga ccaccttgag cggcggcacg actgcagttg aaagagtgat tgccagcagc
     1261 gcgtaggcga gcgggccgcg ccaggagcgc aaggcggcca ggcgggcgct ggcggcaagg
     1321 gcgacgccga gggcgagcac gaagacgatg accagcgttt tcgcccggtc gtgcaggacg
     1381 gtttccagcc agtagttgtc gcgggcaacg aagccgctgc ccggaacatg gaaatggccg
     1441 gtgatccaga agtcgagcgg cgccgggtcg acgagcagca atagccccat cgccgccagc
     1501 ggcagagcca gccagaaccc gaaggcgaag gggcgggacg gcggggggtc agcggccggt
     1561 cgcatcgcca gcggtgctca gcggccgcca ggcgagcagg ccgttacgga aggcatggct
     1621 cgccccctgg tagtaggcga tgtcgcggcg gagcagcggg tcgtctgcgt tactgcggct
     1681 ttcttggctt tcgccgagcg cgtcgtcgtg gcggcgcatg ccgcggcgcg ggctgaggat
     1741 ggcgaggtcc ttgccgtcga acaggccgag gtggatgtag taggcgaaac gagcggc
//
LOCUS       DAOSDX010000667         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22240_length_1796_cov_2.539920, whole genome shotgun sequence.
ACCESSION   DAOSDX010000667 DAOSDX010000000
VERSION     DAOSDX010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22240_length_1796_cov_2.539920"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..>1796)
                     /locus_tag="PLN02_12890"
     CDS             complement(61..>1796)
                     /locus_tag="PLN02_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOI53270.1"
                     /translation="AGSDAASIPDAGVVCKGVYQGKEVLGMRVSFDKRYITLAPLCTV
                     FGLAFHLYDPEGLLGDKKHIGITCALVPYDHPGVDTGRRHFPLNAMFMNGPTRGKDVF
                     MPLDFIIGGPAMAGQGWRMLMECLAAGRSISLPSSNTGMAQMTARAVGGYARVRSQFK
                     MAVGKFEGVEEALTRIGAYTYLMDAVRKMTAGAIDLGEKPSVVSAIAKYHVTERARQV
                     VNDGMDVIGGKGICLGPSNFLGRAYQQIPVAITVEGANILTRSLIIFGQGAIRCHPYL
                     LPEMRAAQNPDARQGLVDFDRALFGHIGFTMKNGWRAFRQGLTGSHFVTVNTDVAPEM
                     RRYYQQLTRFSAAFAFLSDVSLLVLGGSVKRREKLSARLGDILAQMYLISCTLKRYEA
                     EGRRKEDAPLAHWAIWDAMYKAQQAFDGVIANFPVRFIAAVVNRIIFPWGHPYVVPSD
                     EVGHQVAKALIAPSATRDRLTAECYLPLQENEPVGAIELALKATLEAEQIEAKIRAAE
                     KDGRFDGNPRANVRDIAVVAFEAGVINAAEYEVMKRRNHLRDIVVRVDDFPFDYGVAT
                     ANKPADNRMAA"
BASE COUNT          307 a          566 c          611 g          312 t
ORIGIN      
        1 gacctttctg cgctttcaaa aacggcgtgc gggcgccgtc caccacgtag atcgtcttgt
       61 tcatgccgcc atccggttgt cggccggctt gttggccgtc gccacgccgt aatcgaaggg
      121 gaagtcatcg acgcggacga cgatgtcgcg caggtggttg cgccgcttca tcacttcgta
      181 ctcggcggcg ttgatgacgc cggcctcgaa agcgacgacg gcgatgtcgc gcacattggc
      241 gcgcggattg ccgtcgaagc ggccgtcctt ctcggcggcg cggatcttgg cctcgatctg
      301 ctcggcttcc agcgtcgcct tgagcgccag ttcgatggcg ccgaccggtt cgttttcctg
      361 cagcggcagg tagcattcgg cggtcagccg gtcgcgcgtt gccgacggcg cgatcagcgc
      421 cttcgccacc tggtggccga cctcgtccga gggcacgaca tagggatggc cccaggggaa
      481 gatgatgcga ttgacgacgg cggcgatgaa gcgcaccggg aagttggcga tgacgccgtc
      541 gaaagcctgt tgcgccttgt acatcgcgtc ccagatcgcc cagtgggcga gcggcgcgtc
      601 ttccttcctg cggccttcgg cctcgtaacg cttgagggtg caggaaatca ggtacatctg
      661 cgccaggatg tcgccgaggc gcgcggagag cttttcgcgg cgcttgacgc tgccgccgag
      721 gaccagcagc gagacgtcgg agaggaaggc aaaagccgcc gaaaaacgcg tcagttgctg
      781 gtagtagcgc cgcatctccg gcgccacgtc ggtgttgacc gtgacgaagt gcgagccggt
      841 caaaccctgg cggaaagcgc gccagccgtt cttcatggtg aagccgatgt ggccgaacag
      901 cgcccggtcg aaatcaacca gcccctggcg ggcatccggg ttctgcgcgg cgcgcatctc
      961 cggcagcaga tagggatggc agcggatcgc accctggccg aagatgatca gcgaacgggt
     1021 caggatgttg gcgccttcga cggtgatcgc caccggaatt tgctggtagg cacggccgag
     1081 gaagttcgac ggtccaaggc agatgccctt gccgccgatg acgtccatgc cgtcattgac
     1141 cacctggcgg gcgcgttcgg tgacgtggta tttggcgatc gcggagacta ccgacggctt
     1201 ctcgccaagg tcgatggcac cggcggtcat cttgcgcacc gcgtccatca ggtaggtgta
     1261 ggcgccgatg cgggtcagcg cttcctcgac gccttcgaac ttgccgaccg ccatcttgaa
     1321 ttgcgaacgc acccgggcgt agccgccgac ggcgcgcgcc gtcatctgcg ccatgccggt
     1381 gttcgacgag ggcagcgaaa ttgagcgccc ggccgccagg cactccatca gcatgcgcca
     1441 gccctgcccg gccatcgccg ggccgccgat gatgaaatcg agcggcatga agacgtcctt
     1501 gccgcgcgtc gggccgttca tgaacatggc gttgagcggg aagtggcgac gaccggtatc
     1561 gacgcccgga tgatcgtagg ggaccagcgc gcaggtgatg ccgatgtgct tcttgtcgcc
     1621 cagcagccct tccgggtcgt agaggtgaaa cgccaggccg aacaccgtgc acagcggggc
     1681 cagggtgatg tagcgcttgt cgaaagagac gcgcatgccc aggacttcct tgccctgata
     1741 aacgcccttg cagacgacgc cggcatccgg aatggaggcg gcatccgagc ccgccc
//
LOCUS       DAOSDX010000668         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22275_length_1794_cov_3.318574, whole genome shotgun sequence.
ACCESSION   DAOSDX010000668 DAOSDX010000000
VERSION     DAOSDX010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22275_length_1794_cov_3.318574"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..516)
                     /locus_tag="PLN02_12895"
     CDS             complement(175..516)
                     /locus_tag="PLN02_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOI53271.1"
                     /translation="MIYFNVLIEETGESYRCSPQESLLAGMERLGKKGIPVGCRGGGC
                     GVCKVEIVSGSYLKRVMSRDYVSVEDEAADRVLACRVRPTSDITLRVLGKMAKNVCRL
                     PPAQTGLAAAL"
     gene            complement(531..1592)
                     /locus_tag="PLN02_12900"
     CDS             complement(531..1592)
                     /locus_tag="PLN02_12900"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenol 2-monooxygenase domain-containing
                     protein"
                     /protein_id="HOI53272.1"
                     /translation="MSYELTIEPLGQTIEVEDGQTILDAALRAGIYLPHACCHGLCAT
                     CKVQVTDGEVDHGEASTFALMDFEREEGKCLACCARLESDVVIEAEIEDDPDSQNLPV
                     EDYAGEVVRIEALTPTIRGIWIKLDRPLQFQAGQYINLEVEAFPGQSRAFSLANAPDG
                     GDVVELNVRIVPGGQVTAWLHQQLQVGDRLKLSGPYGRFFVKKSANLPLIFIAGGSGL
                     SSPKSMIEELLAEGCTLPMTLVYGQRNRGELYYDAEFRALAEQYPNFSYVPALSDEPA
                     GSDWDGARGFVHEAAKAHFANDFRGHKAYLCGPPAMIEACVTTLMQGRLFERDIYMEK
                     FLSAADASQQLKSPLFKNI"
     gene            complement(1605..>1794)
                     /locus_tag="PLN02_12905"
     CDS             complement(1605..>1794)
                     /locus_tag="PLN02_12905"
                     /EC_number="1.14.13.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018662 - phenol 2-monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenol hydroxylase subunit P4"
                     /protein_id="HOI53273.1"
                     /translation="HLAHSYSRHPDWAQIDFAKAVWLKSGQPFLPDMNKTLAEQGLKH
                     KDVLRLQTPGLNGNGTYF"
BASE COUNT          302 a          621 c          558 g          313 t
ORIGIN      
        1 ttccccgcga catccacgcc gagcacgaag ccctgctgca agccgccatc aaacgcgacg
       61 ccgatcaggc cgccgccctc ctcgccgagc acatcctgat gaccttccgg tcgatcgagg
      121 cgatccccag cgaagaactc aaccgcaagc cggccgcctg aggcgcggcc gcctttacaa
      181 cgcggcggcc aagcccgtct gggctggcgg cagccggcac acgttcttgg ccatcttgcc
      241 cagcacgcgc aacgtgatgt cgctggtcgg ccttacccgg caggccagca cgcgatccgc
      301 cgcttcgtcc tcgacactga cgtagtcgcg gctcatcacc cgcttcagat agctgcccga
      361 aacgatttcc accttgcaca cgccgcagcc gccgccccgg cagccgaccg gaatgccctt
      421 cttgcccagg cgttccatgc cggccagcag cgactcctgc ggcgagcagc ggtagctctc
      481 gccggtttcc tcgatcagca cgttgaaata gatcatcgcc gcgctcccgc tcagatgttc
      541 ttgaacagcg gactcttcaa ctgctgcgag gcatcggccg cggataagaa cttctccatg
      601 tagatgtcgc gctcgaacaa ccggccttgc atcagcgtcg tgacacaggc ttcgatcatc
      661 gccggtggcc cgcacaggta ggccttgtgg ccgcggaagt cgttggcgaa atgggccttg
      721 gccgcctcgt gcacgaagcc acgcgccccg tcccagtccg aaccggccgg ctcgtcggag
      781 agcgccggca catagctgaa attcgggtac tgctcggcga gggcccggaa ctcggcgtcg
      841 tagtaaagct cgccccggtt gcgctggcca tagacgaggg tcatcggtag cgtgcagcct
      901 tcagcgagca gttcctcgat catcgatttc gggctggaca gcccggaacc gccggcaatg
      961 aagatgagcg gcagattggc cgacttcttg acgaagaagc gcccgtaagg accgctcagc
     1021 ttgagccggt cgccgacctg caactgctgg tgcagccagg cggtgacctg gccgcccggc
     1081 acgatgcgca cgttgagttc gacgacgtcg ccgccgtccg gcgcgttggc cagcgagaag
     1141 gcccgcgact ggccggggaa ggcctcgact tccagattga tgtactggcc ggcctggaat
     1201 tgcagcggcc ggtccagctt gatccagatg ccgcggatgg tcggcgtcag cgcttcgatc
     1261 ctgaccacct cgcccgcgta gtcctcgacc ggcaggttct gcgaatcggg atcgtcctcg
     1321 atctcggctt cgatcaccac gtcggattcg agccgggcgc agcaggccag gcacttgccc
     1381 tcctcgcgct cgaagtccat cagcgcaaag gtcgacgcct cgccgtgatc gacttcaccg
     1441 tccgtcacct gcaccttgca ggtggcgcac aggccgtggc agcaggcgtg cggcaggtag
     1501 atgccggcgc gcagggcggc gtcgaggatg gtctggccat cctcgacttc gatcgtctgg
     1561 cccagtggtt cgatggtcag ttcgtagctc atgccgcccc cggctcagaa atacgtgccg
     1621 ttgccgttca gacccggcgt ctgcaggcgc agcacatcct tgtgcttgag cccctgctcg
     1681 gccagcgtct tgttcatgtc cggcaggaag ggctggccgg acttgagcca gaccgccttg
     1741 gcgaaatcga tctgcgccca gtccgggtga cggctgtagg aatgcgccag gtgc
//
LOCUS       DAOSDX010000669         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22282_length_1794_cov_2.671075, whole genome shotgun sequence.
ACCESSION   DAOSDX010000669 DAOSDX010000000
VERSION     DAOSDX010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22282_length_1794_cov_2.671075"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLN02_12910"
     CDS             complement(<1..341)
                     /locus_tag="PLN02_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOI53274.1"
                     /translation="MMNLYSGTTCPFSHRCRIVLYEKGMDFQVIDVDLFSKADEMAAI
                     NPHGRVPVLAERDLVLFEPNIINEYIDERFPHPQLMPADPIMRARARQLLIGMEREIF
                     SHMEIIEKNAKA"
     gene            complement(424..1143)
                     /locus_tag="PLN02_12915"
     CDS             complement(424..1143)
                     /locus_tag="PLN02_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HOI53275.1"
                     /translation="MKKFLIALLFAPVMAFAAGAALHLDKWPGSVSDKAALQNGAKLF
                     VNYCLSCHSASYMRYKNLMDLGLTEAQVKDNLMFAGEKIGDLMTVAARYDEQKKWFGA
                     TPPDLTIVARARGDAGAPKAGADWLYTYLRTFYRDDARPTGWNNVAFAAVGMPHVLWE
                     LQGTQVMNHETHKLELAVPGKMSPAEYDKAVSDLVGFMVWMGEPQQEYRQTLGIFVLL
                     FLAVLFVVAYALKKEYWKDIH"
     gene            complement(1157..>1794)
                     /locus_tag="PLN02_12920"
     CDS             complement(1157..>1794)
                     /locus_tag="PLN02_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOI53276.1"
                     /translation="AAHILALHETGSNNPDGIDIKEKKDEHGIPLDGIPFHPYYTVKD
                     IVGVVVFLMVFSAVMFFAPEGGGYFLETPNFYPADPLKTPPHIAPVWYFTPYYSILRA
                     MVWNFFGLDAKFWGVVAMGASVVVFAFLPWLDRSPVRSIRYRGPITKAMVTLFVICFF
                     ILGYLGTLSPSPMGELVSQICMVFYFAFFLGMPWWSRMDKFKPVPERVTMK"
BASE COUNT          396 a          511 c          549 g          338 t
ORIGIN      
        1 gccttggcgt tcttctcgat gatttccatg tgcgagaaga tttcacgctc catgccgatc
       61 aatagctggc gggcgcgcgc gcgcatgatc gggtcggccg gcatcagttg cggatgcggg
      121 aagcgttcgt cgatgtactc gttgatgatg ttcggttcga acagcacgag gtcgcgttcg
      181 gcgagcaccg gcacccggcc gtgcgggttg atggcggcca tctcgtcggc cttggagaac
      241 aggtcgacgt cgatgacctg aaagtccatg cccttttcgt aaaggacgat gcggcaacgg
      301 tgactgaagg ggcaggtcgt gcccgagtag aggttcatca tgtttaacag ccctcaaatg
      361 agattcggca ccgggggccg accgaagtca aacccacgat gccgtcagca gatccgggaa
      421 ggatcagtga atgtctttcc agtattcctt cttcagcgca taggcaacga cgaagagaac
      481 agccaggaaa agcagcacga agatgccgag cgtctggcgg tattcctgtt gcggttcgcc
      541 catccagacc atgaagccga ccaggtcgga aaccgccttg tcgtattccg ccgggctcat
      601 cttgccggga acggccagtt cgagcttgtg cgtttcatgg ttcatcacct gggtgccttg
      661 cagctcccag aggacgtgcg gcatgccaac ggcggcgaaa gcgacgttgt tccagccggt
      721 cgggcgggca tcgtcacgat agaaggtgcg cagataggta tacaaccagt ccgcaccagc
      781 cttcggcgca cctgcgtcac cacgggcgcg ggcaacgatg gtcagatccg gcggcgttgc
      841 accgaaccac ttcttctgct cgtcatagcg cgctgcaacc gtcatcaggt cgccaatctt
      901 ctcgccagcg aacatcaggt tatccttgac ttgcgcttcg gtcaaaccaa ggtccatcag
      961 gttcttgtag cgcatgtagg acgcactatg gcagctcaag cagtaattga cgaacagctt
     1021 ggcaccattc tgcaacgctg ccttgtcgct gaccgaaccc ggccacttgt cgaggtgaag
     1081 cgccgcaccg gcagcaaaag ccatgaccgg cgcgaagagc aatgcgatga gaaatttttt
     1141 catgttatgc atcctctcac ttcatcgtga cgcgttcggg aaccggcttg aacttgtcca
     1201 tccgggacca ccacggcatg ccgaggaaga aggcgaaata gaagaccata caaatctgcg
     1261 acaccagttc gcccatcggc gacggcgaca gggtgccgag gtaaccgagg atgaagaagc
     1321 agatcacgaa cagggtgacc atcgccttgg tgatcggacc gcggtagcgg atcgaacgca
     1381 ccgggctgcg atccagccag ggcaggaagg cgaagacgac aaccgaagcg cccatcgcaa
     1441 cgacccccca gaacttggcg tcgaggccga agaagttcca caccatggcg cgcaggatcg
     1501 agtagtacgg cgtgaagtac cagaccggcg cgatgtgcgg cggcgtcttc agcggatcgg
     1561 ccgggtagaa gttcggcgtt tccaggaagt agccaccacc ttccggtgcg aagaacatca
     1621 cggcggagaa gaccatcagg aagacgacga cgccgacgat gtccttgacc gtgtagtacg
     1681 gatggaaagg aatgccgtcg agcggaatgc cgtgctcgtc cttcttttcc ttgatgtcga
     1741 taccatccgg gttgttcgag ccggtttcgt gcagcgccag gatgtgggca gcaa
//
LOCUS       DAOSDX010000670         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22338_length_1790_cov_2.989625, whole genome shotgun sequence.
ACCESSION   DAOSDX010000670 DAOSDX010000000
VERSION     DAOSDX010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22338_length_1790_cov_2.989625"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLN02_12925"
     CDS             complement(<1..420)
                     /locus_tag="PLN02_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53277.1"
                     /translation="MKGLFVMRRFLAVLFFIFAGMPAAFAAFDGNAVIDAIVKRGEAA
                     VAAYDPAQPLAAASEFSALYFDIFEGSGMELDLGLKSAALKTELEVLFGALNGNAMRG
                     VPKAELLKSWDTLHSRLDSARALYADESAASAGGSFVK"
     gene            complement(417..>1790)
                     /locus_tag="PLN02_12930"
     CDS             complement(417..>1790)
                     /locus_tag="PLN02_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOI53278.1"
                     /translation="YYDFDGRQWTLDADYEAGQGRRFMAQGWTSGARFSETLRGTGRE
                     IRYRIRLTPHDRLWLYGLDLPSRLPAESVIGPDWQVLSHRPVTQEIHYDMASWLDWEG
                     GGSLDAAQHQRALALPATGNPALRELGARLNREHKSNAAAIARAALISLTNAGYRLDE
                     RQPLPAGDDGIDALWFQTKAGDASAYATAYAFLMRAAGVPARLVSGFRGGKLMALTDY
                     VVVKRSHAHAWVEIWDESNGWRRFDPTDLVAPERFDNTSAKPKTQATPKPQAATPPRQ
                     PNAPSPSAATAEAQPTATPPRLAFMDLPLPDIAGWLARWVLKLDADAQKQLLGGDGSG
                     PLWAWLLGLGVLFGTLLAGAMAAFARWREWRAVPVAQRAWDKSLRLLARHGIAVLPGE
                     CPSRLAARVAASHPAWAEALARLALAYTDWRYAAARDDAPARVVRAARHLNNLILAGT
                     LPDRSSS"
BASE COUNT          257 a          585 c          672 g          276 t
ORIGIN      
        1 cttgacgaag ctgccgccgg cgctcgccgc gctctcgtcg gcgtagagcg cgcgcgccga
       61 atcgagccgg ctgtgcaagg tatcccagga cttgagcagt tccgcctttg gcacgccgcg
      121 catcgcgttg ccgttgagcg caccgaacag gacttccagt tcggtcttca gcgcggcgct
      181 tttgaggccg aggtcgagtt ccatgcccga gccttcgaag atgtcgaaat agagcgccga
      241 aaactcgctt gccgctgcca gcggctgcgc cgggtcgtag gcggcgaccg cagcttcgcc
      301 gcgcttgacg atggcgtcga tcaccgcgtt gccgtcgaag gcggcgaaag cggcgggcat
      361 cccggcgaaa atgaagaaga ggacggcgag gaagcggcgc atgacaaaga gtcctttcat
      421 gacgaagagc ggtcgggcaa ggtgccggcc aggatcaggt tgttcaggtg gcgggcggcg
      481 cggacgacgc gggccggcgc atcgtcgcgc gcggcggcgt aacgccagtc ggtataggcc
      541 agcgccagcc gtgccagcgc ctcggcccag gccgggtggc tggcggcgac gcgtgcggcc
      601 aggcggctcg ggcattcgcc gggcaggacg gcgatgccgt gacgggcgag caggcgcagc
      661 gatttgtccc aggcgcgctg cgcgacgggc acggctcgcc attcgcgcca gcgggcgaaa
      721 gcggccatgg cgccggccag tagcgtgccg aacaggacgc ccaggccgag cagccaggcc
      781 cacaacgggc cgctgccgtc gccgccgagc agttgcttct gggcgtcggc gtcgagtttc
      841 agcacccagc gcgccagcca gccggcgatg tcgggcaagg gcaggtccat gaacgcgagc
      901 cggggcggcg tcgctgttgg ctgagcctcg gctgttgcgg ccgacggcga cggcgcgttc
      961 ggctggcgtg gcggggtggc cgcctgcggc ttcggtgtgg cctgtgtttt cggtttggcc
     1021 gaggtgttat cgaagcgctc cggcgcgacc aggtcggtcg gatcgaaacg gcgccagccg
     1081 ttgctttcgt cccagatttc cacccaggcg tgcgcgtggc tgcgcttgac gacgacgtaa
     1141 tcggtcagcg ccatcagctt gccgccgcga aaaccgctga ccaggcgggc cggcaccccg
     1201 gcggcgcgca tcaggaaggc gtaggcggtg gcgtaggcgc tggcgtcgcc ggccttggtc
     1261 tggaaccaca aggcgtcgat gccgtcgtcg ccggccggca gcggttggcg ttcgtcgagc
     1321 cgatagccgg cgttggtcag cgaaatcagc gcggcgcggg cgatggctgc cgcgttgctt
     1381 ttgtgttccc ggttcagccg ggcgcccagt tcgcgcagcg ccgggttgcc ggtcgccggt
     1441 agcgccagcg cgcgttggtg ctgagccgcg tcgaggctgc cgccgccttc ccaatccagc
     1501 cacgacgcca tgtcgtaatg gatttcctgc gtcaccggcc ggtgcgacag tacctgccag
     1561 tcggggccga tcaccgattc agccggcagc cgcgacggca ggtcgaggcc gtacagccac
     1621 agccggtcgt gcggcgtcag gcggatgcgg tagcggatct cgcggccggt gccgcgcagg
     1681 gtttccgaga aacgggcgcc gctggtccat ccctgcgcca tgaagcggcg cccctggccg
     1741 gcctcgtaat cggcgtcgag cgtccattgc cggccgtcga agtcgtaata
//
LOCUS       DAOSDX010000671         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22599_length_1773_cov_3.487776, whole genome shotgun sequence.
ACCESSION   DAOSDX010000671 DAOSDX010000000
VERSION     DAOSDX010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22599_length_1773_cov_3.487776"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLN02_12935"
     CDS             complement(<1..577)
                     /locus_tag="PLN02_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOI53279.1"
                     /translation="MRFHFPVIIIDEDFRSENASGLGIRALADALQSEGLEVLGVTSY
                     GDLTSFAQQQSRASAFILSIDDEELALEPEETLAELRAFVGEIRHKNAEIPIFLHGET
                     RTSRHIPNDILRELHGFIHMHEDTPEFIARNIKREAHAYLDSLPPPFFRALTHYAADG
                     SYSWHCPGHSGGVAFLKSPVGQMFHQFFGENM"
     gene            complement(648..1214)
                     /gene="dcd"
                     /locus_tag="PLN02_12940"
     CDS             complement(648..1214)
                     /gene="dcd"
                     /locus_tag="PLN02_12940"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOI53280.1"
                     /translation="MAIKSDKWIRRMAQEHGMIEPFAPELVREANGQKIVSYGTSSYG
                     YDIRCAREFKVFTNINSTVVDPKNFDPKSFVEIESDVCIIPPNSFALARTMEYFRIPR
                     SVLTVCLGKSTYARCGIIVNVTPFEPEWEGHVTLEFSNTTPLPAKIYAGEGCAQVLFF
                     ESDEICETSYKDRGGKYQGQEGVTLPKI"
     gene            complement(1289..>1773)
                     /locus_tag="PLN02_12945"
     CDS             complement(1289..>1773)
                     /locus_tag="PLN02_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOI53281.1"
                     /translation="WRDVDYLIVDMPPGTGDTQLSLAQKVPVTGAVIVTTPQDIALID
                     ARKGLKMFEKVNIPILGIVENMSIHVCSNCGHAEHIFGEGGGEQMCKDYGSEFLGSLP
                     LEMSIRQMADGGKPTVVGAPDSPAAEIYRRIARRVAVKVAEKAKDMSSKFPNIVVQNT
                     "
BASE COUNT          320 a          513 c          586 g          354 t
ORIGIN      
        1 gcatgttctc gccgaagaac tggtggaaca tctggccgac cggcgacttc aggaaggcga
       61 cgccacccga gtggccgggg cagtgccagg aataggagcc gtcggcggcg tagtgggtca
      121 acgcgcggaa gaagggcggc ggcagcgaat cgagataggc atgcgcctcg cgcttgatgt
      181 tgcgggcgat gaactccggc gtgtcctcgt gcatgtggat gaagccgtgc agctcgcgca
      241 ggatgtcgtt cgggatgtga cggctggtgc gcgtctcgcc gtgcaggaag atcgggatct
      301 cggcgttctt gtggcggatt tcgccgacga aggcgcgcag ctcggccagc gtctcttccg
      361 gttcgagcgc cagttcctcg tcgtcgatcg acaggatgaa ggccgatgcc cggctctgtt
      421 gctgggcgaa cgaggtcagg tcgccgtagc tggtaacgcc cagcacttcc agcccctcgc
      481 tctgcaaggc gtcggccagg gcgcggatcc ccagcccgga ggcgttttcg gaacggaagt
      541 cttcatcgat gatgatgacc ggaaaatgga agcgcatggt gtatccccaa aagcggcgac
      601 ccgccgcagc gggtcggaga ataagcggtt tacgttacgg aatgcggtca gatcttgggc
      661 agggtgacgc cttcctgccc ctggtacttg ccgccgcgat ccttgtacga ggtctcgcag
      721 atttcgtcgg actcgaagaa cagcacctgg gcgcagcctt cgccggcgta gatcttggcc
      781 ggcagcggcg tggtgttcga gaattcgagc gtgacatggc cttcccattc cggctcgaaa
      841 ggcgtcacgt tgacgatgat gccgcagcgc gcataggtcg atttgccgag gcagacggtg
      901 agcaccgagc gcgggatgcg gaagtattcc atggtgcgcg ccagcgcgaa ggagttcggc
      961 gggatgatgc agacgtcgga ttcgatctcg acgaaggact tcgggtcgaa gttcttggga
     1021 tcgacaacgg tcgaattgat gttggtaaac accttgaatt cgcgcgcgca gcggatgtcg
     1081 tagccgtagc tcgaggtacc ataggagacg atcttctggc cgttcgcctc gcgcaccagt
     1141 tccggcgcga acggctcgat catgccgtgt tcctgcgcca tgcggcgtat ccacttgtcc
     1201 gatttgatgg ccatacggga atcctgcggt caaccagaaa taaaggcggg attttacccg
     1261 cctttattcg ctgcaatgtg taaaaaaatc aggtgttctg gacgacgatg ttggggaact
     1321 tcgaactcat gtccttggcc ttttcggcga ccttgaccgc gacccggcgg gcgatgcgac
     1381 gatagatttc ggcggccggc gaatccggcg cgccaaccac ggtcggcttg ccgccgtcgg
     1441 ccatctggcg gatgctcatt tccagcggca ggctgcccag gaattcgctg ccgtagtcct
     1501 tgcacatctg ctcgccgccg ccttcaccga agatgtgctc ggcgtgaccg cagttcgaac
     1561 agacgtggat gctcatgttc tcgacgatgc cgaggatcgg gatgttcacc ttctcgaaca
     1621 tcttcagccc cttgcgcgcg tcgatcaggg cgatgtcctg cggcgtggtg acgatcaccg
     1681 cgccggtcac cggcaccttc tgcgccagag acaattgcgt gtcgccggtg cccggcggca
     1741 tatcgacgat caggtaatcg acgtcccgcc agc
//
LOCUS       DAOSDX010000672         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22644_length_1771_cov_2.912587, whole genome shotgun sequence.
ACCESSION   DAOSDX010000672 DAOSDX010000000
VERSION     DAOSDX010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22644_length_1771_cov_2.912587"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLN02_12950"
     CDS             <1..336
                     /locus_tag="PLN02_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53282.1"
                     /translation="RQADRAGARGKKRQEVILETSVDRFLSSCRRMNADLRACDTTPP
                     LGDGATLPLARPDGRPALWLRRNSPAAEIFAGSFSLLLAGMSLPRRRFAPAIPSKRAI
                     GRVQLRFQG"
     gene            337..1107
                     /locus_tag="PLN02_12955"
     CDS             337..1107
                     /locus_tag="PLN02_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOI53283.1"
                     /translation="MTQRVFITGASSGIGEALAVYYAAQGACLGLAGRRGDALAALNE
                     RLGGGHACYVLDVTDAAALAAAASDFCARFGTPDIVIASAGVSVGTLSECAEDLAVFR
                     RVMDINVYGMAATFAAFIPAMRAASGERRLVGIASVAGIRGLPGAEAYSASKAAAIAY
                     LESLRLEMRPAGIKVVSIVPGYIATPMTAINPYRMPFLMPADWAAARFARVIAAGKSY
                     AVILWPMGIVAKLLRLLPNGLYDRLFTNAPRKPRGLAR"
     gene            complement(1120..1320)
                     /locus_tag="PLN02_12960"
     CDS             complement(1120..1320)
                     /locus_tag="PLN02_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53284.1"
                     /translation="MSIFDAPISRCEAIHEMVLTDETERECAREHGCPPGRDCPLHGC
                     FAERSGICEEATEILLQTSRLH"
     gene            1421..1624
                     /gene="thiS"
                     /locus_tag="PLN02_12965"
     CDS             1421..1624
                     /gene="thiS"
                     /locus_tag="PLN02_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOI53285.1"
                     /translation="MPELKINGEIRQFPDPLTVAGLIEQLGYAGKRIAVERNGEIVPK
                     SQHAATPLVSGDQLEIVVAVGGG"
     gene            1638..>1771
                     /locus_tag="PLN02_12970"
     CDS             1638..>1771
                     /locus_tag="PLN02_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOI53286.1"
                     /translation="MHQLTIAGKAYRSRLLVGTGKYKDFAETRAAIDASGAEIVTVAV
                     R"
BASE COUNT          294 a          584 c          563 g          330 t
ORIGIN      
        1 cgacaagctg atcgagcggg tgcgcgcgga aaaaagcggc aagaagtaat ccttgaaacc
       61 tcagttgacc gctttctctc ctcgtgtcgc cgcatgaatg ctgacctccg cgcctgcgat
      121 acgacgcctc ctttgggtga tggcgctacg ctgccgctgg cacgtccgga cgggcgcccg
      181 gctttgtggc tgcgccgaaa ctcgccggct gccgaaattt tcgccggctc gttttcgctc
      241 ctccttgcag gcatgagcct gccgcgtcgt cgcttcgcgc cggccatccc gtccaaacgc
      301 gccatcggcc gcgtccaact gaggtttcaa ggataattga cgcaacgcgt attcatcacg
      361 ggcgcctcgt cgggaatcgg cgaggcgctt gccgtttact acgccgccca aggcgcttgc
      421 cttggtctgg cgggacggcg tggcgacgcg ctggcggcgt tgaacgagcg tctgggcggc
      481 gggcatgcct gctacgtgct cgacgtcacc gacgccgcgg cgctggcggc ggcggcgagc
      541 gatttctgcg cgcgtttcgg tacgcccgac atcgtcatcg ccagcgccgg cgtctcggtc
      601 ggcaccctga gcgaatgcgc cgaggacctt gccgtgttcc ggcgcgtcat ggatatcaac
      661 gtctatggca tggcggcgac cttcgccgcc ttcattccgg ccatgcgtgc cgccagcggc
      721 gagcggcggc tggtcggcat cgcctcggtg gccggcatcc gcggcctgcc gggggcggaa
      781 gcctattcgg cgtcgaaggc ggcggccatc gcctacctgg aaagcctgcg tcttgagatg
      841 cggccggctg ggatcaaggt ggtcagtatc gtcccgggct acatcgcaac gccgatgacg
      901 gcgatcaatc cctaccgcat gcccttcctg atgccggcgg actgggcagc cgcccgcttc
      961 gcccgggtca tcgccgccgg caagtcgtat gcagtgattc tctggccgat gggcatcgtc
     1021 gccaagctgc tgcgcctgct gccgaacggg ctttacgacc gcctgtttac caacgcgccg
     1081 cgcaagccgc gcggcctggc gcggtagcgc cgattttcct caatgcaggc gtgaggtctg
     1141 cagcaggatt tcggtagcct cttcgcaaat cccgctgcgt tcggcaaagc agccgtgcag
     1201 cgggcagtcg cgaccgggcg gacaaccatg ttcgcgggcg cattcgcgtt cggtttcgtc
     1261 ggtcagcacc atttcatgga tcgcttcgca gcgcgagatc ggggcgtcga aaatggacat
     1321 ggcaggcacc tccttgagta tctgatttga atatagacgg tttgccggcg attaccggca
     1381 aaatccaatc gccgtggcga ctcctgttaa aatgcctgcc atgcccgaac tcaagatcaa
     1441 tggcgaaatc cgccagtttc ccgacccgct gaccgttgcc ggcctgatcg agcaactggg
     1501 ctacgcgggc aaacgcatcg ccgtcgaaag aaacggcgaa atcgtcccga agagccagca
     1561 cgcggcaacg ccgctggtct cgggcgacca acttgaaatc gttgtcgccg tcggcggcgg
     1621 ctgaaaggga gaaaaccatg catcaactga ccatcgccgg caaggcgtac cgttcccgtt
     1681 tactcgttgg caccggcaag tacaaggatt tcgccgagac ccgcgccgcc atcgacgcct
     1741 ccggcgccga gatcgtcacc gttgccgtgc g
//
LOCUS       DAOSDX010000673         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22695_length_1768_cov_2.423234, whole genome shotgun sequence.
ACCESSION   DAOSDX010000673 DAOSDX010000000
VERSION     DAOSDX010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22695_length_1768_cov_2.423234"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLN02_12975"
     CDS             <1..274
                     /locus_tag="PLN02_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOI53287.1"
                     /translation="ADLVAVGRFVFQRGNQFTGLPACSSCHGAKGHGTPQLPRLAGQH
                     PRYTEEQLRLFNSRERTNDNAVMHTVASKLSDLERHAVAAYIATLD"
     gene            complement(336..1613)
                     /locus_tag="PLN02_12980"
     CDS             complement(336..1613)
                     /locus_tag="PLN02_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HOI53288.1"
                     /translation="MMLMKRRDFLKMSATAGAMASLYGCAAGAKAGGHVVVVGGGYGG
                     ATVAKYLRMWSQGGVQVTLVERNPTFISCPISNLVIGGDKTMEDITVSYDNLRNKWGV
                     RVVQDEVLGVDTGKRTVTLAKGGSLSYDRLVLSPGVDFMFEQIPGLNNAATQQKILHA
                     WKAGPQTVALRQQLEAMPDGGVYAIAIPKAPYRCPPGPYERACLVANYFKKAKPKSKV
                     VILDANEDVQSKKPLFMKAWADLYKGMIEYRNNSEVKDVEVASNTAILEFDQFKADVL
                     NVIPPMRAGNIAAKSGLKLVNNRWVDINWLSMESTSTPGVHVLGDAIFPAPTMPKSGH
                     MANQHGKLAAAAILNLLAGQEPNPAPVVMNTCYSFVDSKNVIHVASVHQYDAATKLVQ
                     PVKGAGGVSAARNELEGKVALAWAKNIWADMLA"
     gene            complement(1627..>1768)
                     /locus_tag="PLN02_12985"
     CDS             complement(1627..>1768)
                     /locus_tag="PLN02_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOI53289.1"
                     /translation="ESKEKLLQKLADFKSGDKPASIMHQISRGYTDEQLRLIAEYFAA
                     QK"
BASE COUNT          285 a          562 c          550 g          371 t
ORIGIN      
        1 tgccgatctg gtcgccgtcg gccgcttcgt cttccagcgc ggcaaccagt tcaccggtct
       61 gcccgcctgt tcgagctgcc atggcgccaa agggcacggc acgccgcaac tgccgcgcct
      121 ggccgggcag catccgcgtt acaccgagga acaattgcgc ctgttcaaca gccgtgagcg
      181 gaccaacgac aacgcggtca tgcatacggt cgcttccaag ctctccgatc tcgagcggca
      241 cgccgtcgcc gcctacatcg cgacgctcga ttgaacgccg gccaccaaat gaaacggccg
      301 cctgcgggcg gccgttttgc gttaccggag aaggttcagg ccagcatgtc ggcccagatg
      361 ttcttcgccc aggccagcgc caccttgcct tcgagttcgt tgcgcgccgc cgagacgccg
      421 cccgcaccct tgaccggttg caccagcttg gtcgcggcat cgtactgatg caccgaagcg
      481 acgtggatga cgtttttcga atcgacgaag ctgtagcagg tgttcatcac caccggcgcc
      541 gggttgggtt cctggccggc aagcaggttg aggattgccg ctgccgccag cttgccgtgc
      601 tggttggcca tgtggcccga cttgggcatg gtcggggccg ggaagatggc gtcgccgagg
      661 acatgcacgc ccggtgtgct ggtcgattcc atcgacagcc agttgatgtc cacccagcga
      721 ttgttgacca gcttcagccc cgacttggcg gcgatattgc cggcccgcat cggcgggatg
      781 acgttgagca cgtcggcctt gaactgatcg aactcgagga tcgccgtgtt gctggcgact
      841 tcgacgtcct tcacctcgct gttgttgcgg tactcgatca tgcccttgta gagatcggcc
      901 caggctttca tgaacagtgg ctttttcgac tggacgtcct cgttggcgtc gagaatgacg
      961 acctttgatt tcggttttgc cttcttgaag tagttggcga ccaggcaggc acgctcgtag
     1021 ggtccgggcg ggcagcggta cggcgccttg gggatggcga tcgcgtagac gccgccatcc
     1081 ggcatcgctt ccagttgctg gcgcagggcg acggtctgcg gaccagcctt ccaggcgtgc
     1141 aggattttct gctgggtggc tgcattgttc aggccgggaa tctgttcgaa catgaaatcg
     1201 acccccggcg acaggaccag gcggtcgtag ctcaagctgc cgcccttggc cagcgtcaca
     1261 gtgcgcttgc cggtgtcgac gccgagcacc tcgtcctgca ccacacgcac gccccatttg
     1321 ttgcgcaggt tgtcgtagct gaccgtgatg tcttccatcg tcttgtcgcc accgatcacc
     1381 aggttggaaa tcgggcagga gatgaaggtc gggttgcgtt cgaccagagt cacctggacg
     1441 ccgccctggc tccacatgcg caggtacttg gcaaccgtcg cgccgccgta gccgccgccg
     1501 accacgacga cgtggccgcc ggccttggcg ccggcagcgc agccgtagag cgaagccatg
     1561 gcgccagcgg tggcgctcat cttgagaaag tcgcgtcttt tcatcagcat cattgtcgtt
     1621 tcccccttat ttctgtgccg cgaagtattc ggcgatcagg cgcaactgct cgtcggtata
     1681 gccacgcgaa atctggtgca tgatcgacgc cggcttgtcg ccgctcttga aatcggcaag
     1741 cttttgcagc agtttttcct tgctttcg
//
LOCUS       DAOSDX010000674         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22697_length_1768_cov_2.354349, whole genome shotgun sequence.
ACCESSION   DAOSDX010000674 DAOSDX010000000
VERSION     DAOSDX010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22697_length_1768_cov_2.354349"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..749
                     /locus_tag="PLN02_12990"
     CDS             108..749
                     /locus_tag="PLN02_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI53290.1"
                     /translation="MRHQIVPVTPFEQNCTLFWCEKTRQAKVIDPGGDVDRILAALAA
                     EGLTLAKVLVTHGHIDHAGGVATLVARTGVPIEGPQAEDAFWIDAMGQQSRMFGFPDT
                     APFTPDRWLVDGDKVRFGEVELDVLHCPGHTPGHVVFFHAPSRLAQVGDVLFQGSIGR
                     TDFPRGDHATLIASIKDKLFPLGDDVAFIPGHGPMSTLGEERRYNPFLSGRFG"
     gene            complement(750..>1768)
                     /locus_tag="PLN02_12995"
     CDS             complement(750..>1768)
                     /locus_tag="PLN02_12995"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOI53291.1"
                     /translation="GFDHRQPWFLAATPLAASSAERIIACFRAGGEDASELEAALRQM
                     KRKLGLIAAPERKLPASGAWDYLLLPVSQARVQAPYTLLGLATRTVIVATEVQTHSDR
                     DWALANACTFCTGEYLLTRSANGRKADTTRQKMLRLLALIAQDADTAELDEVFRQESK
                     LSYSLLRLVNSAAMAPRTPITCFSQAINMLGRRQLQRWLQLLVYADPDNDLHPNPLLY
                     KAAVRGRLMELLTQNFAAQAEPATTGDGAFMIGSFSLLDVLLNMSMPEILQQLPLAPA
                     INAALAHHAGSAGKLLRALDAADRRNYAIAAGLLDELAIAPDRFRDAQMQALQWAGRI
                     HVAA"
BASE COUNT          271 a          568 c          623 g          306 t
ORIGIN      
        1 gaacgggcgg cgggcccgga tggcaggcag tgtaagggcc gggctgacgg cgggcaagcc
       61 tgccgccatg cgataatcgc cgcccttgtc cattcttccg tttcgccatg cgccatcaga
      121 tcgttcccgt cactcccttc gagcagaact gcaccctgtt ctggtgcgag aagacccgtc
      181 aggcgaaggt catcgatccc ggcggcgatg tagatcgcat cctcgccgcg ctggcggcag
      241 aggggctgac gctggccaag gtgctggtga cacacggcca catcgaccac gccggcggtg
      301 tcgccacgct ggtcgccaga accggcgtgc cgatcgaggg gccgcaggcc gaagacgcct
      361 tctggatcga cgccatgggg cagcagagcc ggatgttcgg cttcccggac acggcgccct
      421 tcaccccgga ccgctggctg gtcgatggcg acaaagtgcg tttcggcgag gtcgaactcg
      481 acgtgctgca ctgccccggc cacacgccgg gacacgtcgt cttcttccat gcgccgagcc
      541 gcctggcgca ggttggcgac gtgctgttcc aaggctcgat cgggcgcacc gatttcccgc
      601 gtggcgacca cgcgacgctg atcgcctcga tcaaggacaa gctgttcccg ctcggcgacg
      661 acgtcgcctt cattcccggt cacgggccga tgtcgacgct gggcgaggag cgccgctaca
      721 acccctttct cagcggtcgt ttcggctgat caagcggcga cgtggatgcg cccggcccat
      781 tgcagtgcct gcatctgggc gtcgcggaaa cggtccggag caatcgccag ttcgtcgagc
      841 agcccggcgg cgattgcgta attgcgccgg tcggcggcat ccagcgcccg cagcagtttg
      901 ccggccgagc cagcgtgatg cgccagggca gcgttgatcg ccggggccag cggtagttgc
      961 tgcaggattt ccggcattga catgttgagc aggacatcga gcagcgaaaa gctgccgatc
     1021 atgaaggcgc cgtcgccagt tgtggccggc tcggcctggg cggcgaagtt ctgcgtcagc
     1081 agttccatca gccggccgcg caccgccgcc ttgtagagca gcgggttggg gtggaggtcg
     1141 ttgtccggat cggcgtaaac caggagttgc aaccagcgct gcaactgccg gcggccaagc
     1201 atgttgatcg cctgggagaa gcaggtgatc ggcgtgcgcg gcgccatcgc cgccgaattg
     1261 accaggcgca gcaggctgta ggagagtttg ctctcctggc ggaagacttc gtcgagctcg
     1321 gcggtgtcgg cgtcctgggc aatcagggcg agcagcctga gcatcttctg gcgcgtcgtg
     1381 tcggccttcc tgccgttggc gctgcgcgtc agcaggtatt cgccggtgca gaaggtgcag
     1441 gcgttggcca gtgcccagtc gcggtcgctg tgcgtctgca cctcggtggc gacgatcacc
     1501 gtgcgcgtcg ccaggccgag cagcgtgtac ggcgcttgga cacgtgcctg actgaccggc
     1561 agcagcaggt aatcccaggc gccgctcgcc ggtagtttgc gctccggggc agcgatcagg
     1621 cccagcttgc gtttcatttg gcgcagggcg gcttccagtt cgctggcgtc ttcccccccg
     1681 gcgcggaagc aggcgatgat gcgctcggcg ctgctggcgg cgagcggcgt ggcggccaga
     1741 aaccagggct ggcgatggtc gaaaccat
//
LOCUS       DAOSDX010000675         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22710_length_1767_cov_2.996495, whole genome shotgun sequence.
ACCESSION   DAOSDX010000675 DAOSDX010000000
VERSION     DAOSDX010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22710_length_1767_cov_2.996495"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1767)
                     /locus_tag="PLN02_13000"
     CDS             complement(<1..>1767)
                     /locus_tag="PLN02_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOI53292.1"
                     /translation="APALWQQIHVEAGWEEAVEAVLRERLSAIACDDPGKLDAWLHDR
                     PQARLSVILDGSTDGLAQGEKTLLDQVRSDDPRIRAALADWLGNVATVETLAEALTRR
                     ETLAPGAVVVTRGGDLLTRSSVTFFAPDQGEHGLLERQREIEALAEGIALAEEKGETL
                     REQAALLDEQYADKQEEIDETRQYVTDRQQRVHHVQLEVLKLTQALARYNEQKERIAE
                     QLAELAEEEEGEREREMAADERIEEIRDGLNRIRVLVAGAQSRLDECERAVRAEREKN
                     ADFDRALREAQFSAREAEGKLQDIFAQQAVAQRELSRIERDRAACAEQVEAAPADVME
                     EQLQQALAARQQAEQELAARRDALEAATNALRALEEQRLKIEQGLEPLRAKIGDLKLK
                     EQAASLNAEQFAMQLLEASADEAALLPLLEMLGNAARPPLLQGEITRLGNAIAELGAV
                     NLAALQELETAAERKGYLDMQAADLTEAMETLENAIRRIDRETRDLLQSTFDTVNGHF
                     GQLFPELFGGGRAELVMTGEEILDAGVQVIAQPPGKKNSTIHLLSGGEKALTAIALVF
                     SMFQLNPAPFCLMDEVDAPLDD"
BASE COUNT          214 a          632 c          590 g          331 t
ORIGIN      
        1 tgtcgtccag cggcgcatcg acttcgtcca tcaggcagaa cggcgccggg ttcaactgga
       61 acatcgagaa gaccagcgca atcgcggtca atgccttctc gccaccggac agcaggtgga
      121 tggtcgagtt cttcttgccc ggcggctggg cgatcacctg cacgccggcg tcgaggattt
      181 cctcgccggt catcaccaat tcggcgcggc cgccgccgaa gagttccggg aacagctggc
      241 cgaaatgccc attgaccgtg tcgaaggtgc tctgcagcag atcgcgcgtt tcgcggtcga
      301 tgcggcggat ggcgttttcc agggtttcca tcgcctcggt caggtcggcc gcctgcatgt
      361 cgaggtagcc cttgcgctcg gcggccgttt ccagctcctg cagcgccgcc aggttgaccg
      421 cgccgagttc ggcgatggcg ttgcccagcc gggtgatctc gccctgcagc aagggcggcc
      481 gcgccgcatt tcccaacatc tccagcagcg gcagcagcgc cgcctcgtcg gcgctggctt
      541 cgaggagctg catggcgaac tgctcggcgt tgagcgaggc tgcctgttcc ttcagcttga
      601 gatcgccgat cttcgcgcgc agcggctcca gcccctgctc gatcttcagc cgctgctctt
      661 ccagcgcgcg cagcgcattg gtcgccgctt ccagcgcgtc gcgcctcgcc gccagctcct
      721 gctcggcctg ctggcgggcg gccagcgcct gctgcaactg ctcctccatg acgtcggccg
      781 gcgccgcctc gacctgctcg gcgcaggcgg cgcggtcgcg ctcgatgcgg ctcagttcgc
      841 gctgggcgac cgcctgctgg gcgaagatgt cctggagctt gccctcggcc tcgcgcgccg
      901 agaactgcgc ctcgcgcaac gcccggtcga aatcggcgtt cttctcgcgc tcggcgcgca
      961 cggcgcgttc gcattcgtcg agccgggact gggcgccggc gaccaggacg cggatgcggt
     1021 tgaggccgtc gcggatttcc tcgatccgct cgtcggcggc catttcgcgt tcgcgctcgc
     1081 cctcttcttc ttcggccagt tcggccagtt gctcggcgat gcgttccttc tgctcgttgt
     1141 agcgggccag cgcctgcgtc agcttgagca cttccagttg cacgtggtgc acgcgctgct
     1201 ggcggtcggt gacgtactgg cgcgtctcgt cgatttcttc ctgcttgtcg gcgtactgct
     1261 cgtcgagcag cgccgcctgc tcgcgcaggg tctcgccctt ttcctcggcg agcgcgatgc
     1321 cctcggccag cgcttcgatc tcgcgctggc gttcgagcag gccgtgctcg ccctggtcgg
     1381 gcgcgaagaa ggtgacgctg ctgcgcgtca gcagatcgcc gccacgggtg acgacgacgg
     1441 cgccgggcgc cagcgtctcg cgccgggtca acgcctcggc cagcgtttcg acggtggcga
     1501 cgttgcccag ccagtcggcg agcgcggcgc ggatgcgcgg atcgtcgctg cgcacctggt
     1561 cgagcaaggt cttttccccc tgcgccaggc catcggtgga accgtcgagg atcaccgaca
     1621 gccgcgcctg cggccggtca tgcagccagg cgtcgagctt gccgggatcg tcgcaggcaa
     1681 tcgccgacag gcgttcgcgc agcaccgctt cgaccgcctc ttcccaaccg gcctcgacat
     1741 ggatctgctg ccacagcgcc ggcgcct
//
LOCUS       DAOSDX010000676         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22884_length_1757_cov_1.876028, whole genome shotgun sequence.
ACCESSION   DAOSDX010000676 DAOSDX010000000
VERSION     DAOSDX010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22884_length_1757_cov_1.876028"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="tuf"
                     /locus_tag="PLN02_13005"
     CDS             <1..962
                     /gene="tuf"
                     /locus_tag="PLN02_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOI53293.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHIL
                     LARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIVIGSALKAL
                     EGDQSDIGEPSILKLAAALDSYIPTPERAIDKPFLMPVEDVFSISGRGTVVTGRVERG
                     IIKVGEEIEIVGIKPTQKSTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVERGQVL
                     AKPGSIKPHTKFSAEIYVLSKDEGGRHTPFFNGYRPQFYFRTTDVTGSIELPAGTEMV
                     MPGDNVSIKVSLIAPIAMDEGLRFAIREGGRTVGAGVVAKIEE"
     gene            973..1281
                     /gene="rpsJ"
                     /locus_tag="PLN02_13010"
     CDS             973..1281
                     /gene="rpsJ"
                     /locus_tag="PLN02_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOI53294.1"
                     /translation="MQSQKIRIRLKAFDYKLIDQSALEIVETAKRTGAVVKGPVPLPT
                     SIERFDILRSPHVNKTSRDQFEIRTHRRLMDIMDPTDKTVDALMKLDLPAGVDVEIKL
                     "
     gene            1405..>1757
                     /locus_tag="PLN02_13015"
     CDS             1405..>1757
                     /locus_tag="PLN02_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOI53295.1"
                     /translation="MSIGLVGRKVGMTRIFTDSGAAVPVTVLEMSNNRVTQVRTPETD
                     GYSAVQLAYGSRRNSRVNKAEAGHFVKAGVEAAAILREFRVSPEVAAQYQPGATVSVE
                     VFQAGQKVDVSGVTQG"
BASE COUNT          352 a          596 c          509 g          300 t
ORIGIN      
        1 acgcccacgt cgactgcccc ggccacgccg actacgtcaa gaacatgatc accggcgccg
       61 cgcagatgga cggcgccatt ctcgtcgtct cctccgccga cggccccatg ccgcagaccc
      121 gcgagcacat cctgcttgcg cgtcaggttg gcgtgcccta catcatcgtt tacatgaaca
      181 aggccgacat ggtcgacgac gccgagctgc tcgagcttgt cgaaatggag gttcgcgagc
      241 tcctgtccaa gtacgacttc cctggtgacg acacccccat cgtcatcggt tccgccctga
      301 aagccctcga aggcgaccag agcgacatcg gcgagcccag catcctcaag ctggctgctg
      361 cgctcgacag ctacatcccc acccccgagc gcgccatcga caaacccttc ctcatgcccg
      421 tcgaggacgt cttctccatt tccggtcgcg gcaccgtcgt caccggtcgt gtcgagcgcg
      481 gcatcatcaa ggttggcgaa gagatcgaga tcgttggcat caagcccacc cagaagagca
      541 cctgcaccgg cgtcgagatg ttcaggaagc tgcttgacca gggtcaggcg ggtgacaacg
      601 tcggcgtcct gctgcgcggc accaagcgtg aggaagtcga gcgtggccag gttctcgcca
      661 agcccggcag catcaagccg cacaccaagt tcagcgccga gatctatgtc ctgtcgaagg
      721 acgaaggtgg tcgtcacacc ccgttcttca acggttatcg tccgcagttc tacttccgca
      781 ccaccgacgt caccggctcg atcgagttgc cggccggcac cgagatggtg atgcctggcg
      841 acaacgtcag catcaaggtc tcgctgatcg ccccgatcgc catggacgaa ggtctgcgtt
      901 tcgccattcg cgaaggcggt cgtaccgtcg gtgccggtgt cgtcgccaag atcgaagagt
      961 aaggttcatt ccatgcaaag ccagaaaatc cgcattcgcc tcaaggcgtt cgactacaag
     1021 ctgatcgacc agtcggccct cgagatcgtc gagaccgcca agcgcaccgg cgccgtggtc
     1081 aagggcccgg tcccgctgcc gacctcgatc gagcgcttcg acatcctgcg ctcgccgcac
     1141 gtcaacaaaa ccagccgcga ccagttcgag atccgcaccc atcgccgcct gatggacatc
     1201 atggatccca ccgacaagac ggtggatgcc ttgatgaagc tggacctgcc cgccggcgtg
     1261 gacgtcgaaa tcaagttgta aaaagcgggc ggcatgggta taatgccgcc ccttctgctt
     1321 caccaagtcg ccggtcaata gcaatcggca cctacaaccc ttgtccgggg cgcacgttcc
     1381 gggccagaga taggaaatac gaacatgagt atcgggctcg ttggccgcaa ggtcggcatg
     1441 acccgcatct tcaccgacag cggcgcagcc gttccggtga cggtgctgga aatgtccaat
     1501 aatcgcgtca cgcaagtgcg caccccagaa acggatggct attccgccgt tcagcttgcc
     1561 tacggcagtc gccgcaacag ccgcgtcaac aaggccgagg cgggtcactt cgtcaaggcc
     1621 ggcgtcgagg cggccgccat cctccgcgag tttcgcgtca gccccgaagt ggctgcgcag
     1681 taccagccgg gcgcaaccgt ctccgtcgag gtgttccagg ccggccagaa ggtcgacgtc
     1741 agtggcgtca cccaggg
//
LOCUS       DAOSDX010000677         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22920_length_1755_cov_2.881176, whole genome shotgun sequence.
ACCESSION   DAOSDX010000677 DAOSDX010000000
VERSION     DAOSDX010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22920_length_1755_cov_2.881176"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLN02_13020"
     CDS             complement(<1..786)
                     /locus_tag="PLN02_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOI53296.1"
                     /translation="MERDQAMKFMHDLLRLMTQKRGSDLFITAGFPPAIKIDGKITPV
                     SNQVLTSQHTAELARAIMNDRQAAEFESSRECNFAISPTGIGRFRVNALVQQGRVAVV
                     CRTINMTIPTLDDLGLPAVLKDLAMTKRGLIIFVGGTGTGKTTSLAALVDYRNENSYG
                     HIITIEDPIEYVHEHKNCIITQREVGVDTDDWGPALKNTLRQAPDVILMGEIRDRNTM
                     DYAIAFAETGHLALATLHANSTNQAIDRIINFFPEERRAQLLMD"
     gene            complement(817..>1755)
                     /locus_tag="PLN02_13025"
     CDS             complement(817..>1755)
                     /locus_tag="PLN02_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOI53297.1"
                     /translation="RINLPPMEHKDVHGMVYDIMNDAQRKVYEETLECDFSFEIPNLA
                     RFRVNAFNQQRGAAAVFRTIPSKVLTLEELNCPRIFKDIAEYPRGIVLVTGPTGSGKS
                     TTLAAMVNHVNENDYAHILTIEDPIEFVHESKKCLINQREVGPQTLSFANALRSALRE
                     DPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRIVDVFPAEEKEMVR
                     AMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSAIQTGQA
                     LGMQTLDQCLIDLVRRNVVSSSEARVRAANKDAFPA"
BASE COUNT          297 a          521 c          559 g          378 t
ORIGIN      
        1 gtccatcagc agctgagcgc ggcgctcttc agggaagaag ttgatgatcc ggtcgatcgc
       61 ctggttggtg ctgttggcgt gcagcgttgc cagcgcgagg tgaccggttt cggcgaaggc
      121 gatggcgtaa tccatggtgt tgcggtcgcg gatttcgccc atcaggatga catccggtgc
      181 ctgacgcagg gtgtttttca gcgccggtcc ccagtcgtcg gtatcgacac cgacttcgcg
      241 ctgcgtgatg atgcagttct tgtgctcgtg cacgtattcg atcggatcct cgatcgtgat
      301 gatgtgaccg tagctgttct cgttgcggta atcgaccagt gctgccagcg aggtcgtctt
      361 gccggtaccg gtaccgccga caaagatgat caggccacgc ttggtcatcg ccagatcctt
      421 cagaacggct ggcagaccga ggtcgtcgag ggtcgggatg gtcatgttga tggtccggca
      481 gacgacggcg acgcgcccct gctgaaccag tgcattgacc cggaaacgac cgatgccggt
      541 cggcgaaatg gcgaagttgc attcacgcga cgattcgaac tccgccgcct ggcgatcgtt
      601 catgatcgcc cgcgccagtt ccgccgtatg ctgcgaagtc agcacttgat tggaaaccgg
      661 cgtgatcttg ccgtctatct tgatcgccgg cgggaagccg gcggtgatga aaaggtcgga
      721 gccacgcttc tgcgtcatca gacgcagaag atcgtgcatg aatttcatcg cctgatcgcg
      781 ttccatgtat tccccttaat tgcgtccttc cgccgctcag gccgggaagg cgtccttgtt
      841 ggcggcacgc acgcgcgcct cgctgctgga aactacgttg cgccgcacca gatcgatcag
      901 acactggtcc agcgtctgca tgcccagcgc ctgaccggtc tggatcgccg aatacatctg
      961 ggcgatcttg ttttcgcgga tcaggttgcg gatcgccggc gtgccgatca tgatttcgtg
     1021 cgccgcaaca cgcccctggc cgtccttggt cttcagcagc gtctgcgaga tcaccgcgcg
     1081 cagcgattcg gagagcatgg cgcggaccat ttccttttcc tcggccggga agacgtcaac
     1141 gatgcggtcg atggtcttcg ccgccgacga ggtatgcagg gtgccgaaca ccaggtggcc
     1201 ggtctcggcg gcggtcagcg ccagacggat ggtttccagg tcgcgcattt cgccgaccag
     1261 gatgacgtcc ggatcctcgc gcagcgccga gcgcagcgcg ttggcgaagg acagcgtctg
     1321 cggcccgact tcgcgctggt tgatcaggca cttcttcgac tcatggacga attcgatcgg
     1381 gtcctcgatg gtcaggatgt gcgcgtagtc gttttcattg acgtggttaa ccatcgccgc
     1441 cagcgtcgtc gatttgccgg agccggtagg cccggtaacc agcacgatgc cgcgcgggta
     1501 ctcggcgatg tccttgaaga tgcgcgggca attcagttcc tcgagcgtca acaccttgct
     1561 cggaatggtc cggaaaaccg ccgccgcccc ccgctgctgg ttgaaggcat tgacccggaa
     1621 gcgcgccagg ttgggaatct cgaacgagaa gtcgcactcc agggtttctt cgtaaacctt
     1681 gcgctgggcg tcgttcatga tgtcgtacac catgccgtgc acgtccttgt gctccatcgg
     1741 cggcaggttg atgcg
//
LOCUS       DAOSDX010000678         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_22941_length_1754_cov_2.814008, whole genome shotgun sequence.
ACCESSION   DAOSDX010000678 DAOSDX010000000
VERSION     DAOSDX010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22941_length_1754_cov_2.814008"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLN02_13030"
     CDS             <1..583
                     /locus_tag="PLN02_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI53298.1"
                     /translation="LKRMLAYSAISHMGFMLLGIVTGVVSGDARYALNAYSSSMFYVI
                     SYVLMSAGTFGVVLLMARAGFEADNLDDYKGLNKRSPWFAAIMMMLMFSMAGVPFFIG
                     FFAKFSVLQAVVAADYLWLAIVAILFSLIGAFYYLRVVKLMYFDAPADETPIAAGLDM
                     RLLISVNGLAVAVLGVFPQVLMSLCAYALLRSL"
     gene            645..1184
                     /locus_tag="PLN02_13035"
     CDS             645..1184
                     /locus_tag="PLN02_13035"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOI53299.1"
                     /translation="MEMDSHLKETEVESESVFKGVMLDVRRDRVRLPDGKISVREYIR
                     HPGAVVILAQLPNGSLLFERQFRYPLRRVFLELPAGKIDPGETLLDTAQRELLEETGY
                     RAAEWTHLGTMHPCIGYADERIEIFLASGLHSLGAQALDHNEFLDVLELSPADARQAI
                     FNGEITDAKTIAALYWLTR"
     gene            complement(1223..1408)
                     /locus_tag="PLN02_13040"
     CDS             complement(1223..1408)
                     /locus_tag="PLN02_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53300.1"
                     /translation="MIDLAVSGDLPFHESLAVFLGVTGTQWLIEGDADPFIALLLALA
                     VGVAIGLARYLRSIHRD"
     gene            complement(1405..1572)
                     /locus_tag="PLN02_13045"
     CDS             complement(1405..1572)
                     /locus_tag="PLN02_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53301.1"
                     /translation="MTAMTNPASSQEMEEDIMLESNIKAFIAAARYYLLGLLPPPATQ
                     QLATIPARRQA"
BASE COUNT          316 a          492 c          548 g          398 t
ORIGIN      
        1 cctgaaacgg atgctggcct attcggcgat ctcgcacatg ggcttcatgc tgctcggcat
       61 cgtcaccggc gtcgtttccg gggatgcgcg ctacgccctc aacgcctaca gctcgtcgat
      121 gttctacgtg atttcctacg tcctgatgtc ggccggtacc ttcggtgtcg tcctgctgat
      181 ggcgcgtgcc ggttttgagg ccgacaatct cgacgactac aagggcttga acaagcgtag
      241 cccgtggttc gcggcgatca tgatgatgct gatgttctcg atggccgggg tgccgttctt
      301 catcggcttc ttcgccaagt tctcggtgct gcaggcggtc gtcgctgccg attatctgtg
      361 gctggcaatc gtcgccatcc tcttctcgct gatcggcgcc ttctactacc tgcgcgtcgt
      421 caagctgatg tacttcgatg cgcccgccga cgaaaccccg attgcggccg gtctcgacat
      481 gcgcctgctg atttcggtca acggtctggc ggtggccgtg cttggcgtct tcccgcaggt
      541 gctgatgtcg ctttgcgctt acgccctgtt gcgctcgctg taaatcgagt tttcaattcg
      601 aaacgacgcc ccgctggtcg gggcgttttt tttgggagtg gaccatggag atggatagtc
      661 acctgaagga aaccgaggtc gagagcgaat cggtattcaa aggcgtcatg cttgacgtca
      721 ggcgagaccg tgtgcgtctg cccgacggca agatctcggt cagggaatat atccgccatc
      781 ccggcgctgt ggtgattctg gcgcagttgc ccaacggtag cctgctgttc gagcggcagt
      841 tccgttaccc gttgcggcgg gtctttctgg agttgcccgc gggcaagatc gatcctgggg
      901 aaaccctgct tgataccgct caacgggaac tgctcgaaga aaccgggtat cgggctgcgg
      961 aatggacaca cctggggacg atgcatcctt gtatcggcta tgccgacgaa cggatcgaga
     1021 tattccttgc cagtggcctg cattcgcttg gcgcgcaggc gctcgatcac aatgaattcc
     1081 tggatgttct ggagctttcg ccggccgatg ccaggcaagc gatattcaac ggcgagataa
     1141 cggacgccaa gacgattgcc gcgctctatt ggctgacgcg ctgaggcaga taagaaagac
     1201 ctgctgtagg cggcggggcg attcagtccc ggtgaatcga gcgcagatat cttgccagac
     1261 caattgccac accaaccgcc agcgccagca gcagtgcaat aaacgggtcc gcgtcgccct
     1321 caatcagcca ttgcgtcccg gtgacgccaa ggaataccgc cagactctcg tggaaaggca
     1381 gatcacccga cacggcgaga tcgatcaagc ttggcgcctg gcaggtatgg ttgccagttg
     1441 ctgagttgct ggcggaggca gaaggccgag caggtaatag cgggcggcgg caatgaatgc
     1501 cttgatgttg ctttcgagca tgatgtcctc ttccatttcc tgggatgaag ccgggttcgt
     1561 catggctgtc attctaggta atacctctgc tcggggctgt gctggctagg ttgcggtgaa
     1621 gtaaccgcaa ggctttgtat gatgacggtg tgtaaccggc aactactggt gcgctttcca
     1681 gctcagggtg aagcgggcac caccaagcgg cgatgcggcg gcgtaggcgg ttccgccgtg
     1741 cagttcgaga acgc
//
LOCUS       DAOSDX010000679         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23014_length_1749_cov_3.745573, whole genome shotgun sequence.
ACCESSION   DAOSDX010000679 DAOSDX010000000
VERSION     DAOSDX010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23014_length_1749_cov_3.745573"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLN02_13050"
     CDS             complement(<1..886)
                     /locus_tag="PLN02_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53302.1"
                     /translation="MLATALTVIAIVTQDQSALRAAPRNSAAQQAVLWQGDSLEIRGE
                     KGDFLQVYDHRRERAGYIRASQVRVQSLQPAAAPELLAVLRFLKDSPGSEALGIAYAA
                     AYLRAAPASAIDGEPFAALGEMADRLARRASGGRSGKAAEAAAAHVEVAAHYGVRLLS
                     HERDGTVRLCSDGEAQRRVFTLSATEIHKALAALSLTEHDCVPPTLTPSERFAYDNWR
                     ADLLERVALHALPGMLKNRLHLRRAGVWASLAYQRARRPELPATAAAEAARRSLDELA
                     AIDPGELMESDAAAYSDAA"
     gene            complement(889..>1749)
                     /locus_tag="PLN02_13055"
     CDS             complement(889..>1749)
                     /locus_tag="PLN02_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOI53303.1"
                     /translation="IGVDITVRYALEPASLASFARNLPDDIGGQVVEPAAQGVMYKTL
                     ARYTVREIFSTKRQEIRDSMEGELRAALARDGIKLQSVLIGKVELPEEYRAGMDRLLA
                     EELASEKMLYTLQLKEKQVKESDLDAEADKVRREKAAEAAGQEQIIAAKAQAEAMKHI
                     LPFKQKQIEQRGLEAEADKISRIKAAEANAQARQIEAQGEAESRRKLADAEAYRQEKL
                     GKIASEQLERDGALIQKNPLLIQKAMADKLSDKVSVIIAPPPAAGGFIGSALLGQSAA
                     QPVATANEGE"
BASE COUNT          208 a          600 c          613 g          328 t
ORIGIN      
        1 tggcggcgtc gctgtaggcg gcggcatcgc tttccatcag ttcgccggga tcgatcgccg
       61 ccagttcgtc gagcgagcgg cgcgccgcct cggccgccgc cgtcgccggc agttcgggac
      121 ggcgcgcccg ctggtaggcg aggctcgccc agacgccggc tcggcgtagg tgcaggcggt
      181 tcttcagcat ccccggcagg gcgtgcagcg ccacccgttc gagcaggtcg gcacgccagt
      241 tgtcgtaggc aaagcgttcg ctcggggtca gcgtcggcgg cacgcaatcg tgctcggtca
      301 gcgacagtgc cgccagcgcc ttgtggattt ccgtcgccga cagcgtgaac acccggcgct
      361 gggcctcgcc atcgctgcac aggcggaccg tgccgtcgcg ctcgtggctg agcaggcgca
      421 ccccgtagtg ggccgccact tcgacatgcg ccgccgccgc ttcggcggcc ttgccgctac
      481 gcccgccact cgcccggcgg gccaggcggt cagccatttc gccgagggcg gcgaagggtt
      541 cgccgtcgat cgcactggcc ggcgcggccc gcaggtaggc ggcggcatag gcgataccga
      601 gcgcttccga gcccgggctg tccttgagga agcgcagcac cgccagcagc tccggcgctg
      661 ccgcgggctg cagcgactgg acacggacct ggctggcgcg gatgtagccg gcgcgctcgc
      721 ggcggtggtc gtagacctgc aggaaatcgc ccttctcgcc acggatttcc aggctatcgc
      781 cctgccacag cacggcctgc tgggcggcgg agttgcgcgg cgcggcgcgc agcgccgact
      841 ggtcctgggt gacgatggcg atcacggtca aggcggtggc cagcatgctc attctccttc
      901 gttggcggtg gcgaccggct gggcggcgct ctggccgagc agggcgctgc cgatgaaacc
      961 gccggcggcg ggcggcggcg cgatgatcac cgagaccttg tcggacagct tgtcggccat
     1021 cgccttctgg atcagcaacg ggttcttctg gatcagcgcg ccatcgcgtt cgagttgttc
     1081 gctggcgatc ttgcccagct tttcctggcg gtaggcctcg gcgtcggcca gcttgcggcg
     1141 cgattcggct tcgccctggg cctcgatctg gcgcgcctgg gcattcgcct cggcggcctt
     1201 gatgcggctg atcttgtcag cctcggcttc caggccgcgc tgttcgatct gcttctgctt
     1261 gaacggcagg atgtgcttca tcgcctcggc ttgcgccttg gcggcgatga tctgctcctg
     1321 gccggcggct tccgccgcct tttcgcggcg caccttgtcg gcttcggcgt cgaggtcgct
     1381 ttccttgacc tgtttttcct tgagctgcag cgtgtacagc atcttctcgc tggccagttc
     1441 ctcggccagc agacgatcca tgccggcacg gtattcttcc ggcagttcca ccttgccgat
     1501 cagcaccgac tgcagcttga tgccgtcgcg ggccagagcg gcgcgcagtt cgccttccat
     1561 gctgtcgcgg atttcctggc gcttggtgga gaagatctcg cgcaccgtgt agcgcgccag
     1621 cgtcttgtac atgacgccct gcgccgccgg ctcgaccacc tggccgccga tgtcgtcggg
     1681 caggttgcgg gcgaagctgg ccagactcgc cggttcgagt gcgtagcgaa cggtgatgtc
     1741 gacgccgat
//
LOCUS       DAOSDX010000680         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23079_length_1746_cov_2.098167, whole genome shotgun sequence.
ACCESSION   DAOSDX010000680 DAOSDX010000000
VERSION     DAOSDX010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23079_length_1746_cov_2.098167"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PLN02_13060"
     CDS             complement(<1..1336)
                     /locus_tag="PLN02_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOI53304.1"
                     /translation="MSALSLVLLLLGASVLAVVIFRRFNLPPVLGYLAVGSMIGPHAF
                     NLMSDIHRAEYIAEFGVVFLMFSIGLEFSLPKLYAMKRIVFGLGLLQVLLTMLVVLLL
                     VMALGIGWQLGIALGAVFAMSSTAVLSKLLAERMQLDSVHGRQIVGVLLFQDLAVVPL
                     LVLLPSLTESPEKLAMLMGVAMLKAAAVLALILVFGQKLMRKWFHFVARAKSAEVFVL
                     NVLLITLGLATITEHAGLSLALGAFVAGMLIAETEFRMQVEEDIKPFRDVLMGLFFVT
                     IGVKLDLHILVGLWWQVMLVLVLLLVIKALIVGLLSWKLGASSGNAIRSGLWLCAGGE
                     FGFVLLGVITHLPKGIEQVVLTSLVLSMLIAPFIVQYSERLVMRFVASEWLLRSMQVT
                     QIAAQSMTSEKHAILCGFGRNGQYLARFLAQEGIQSIALDLDPDRVREAAGGG"
     gene            1390..>1746
                     /locus_tag="PLN02_13065"
     CDS             1390..>1746
                     /locus_tag="PLN02_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOI53305.1"
                     /translation="MNPNPSPACFSPERALLLGRQTLAIEAAAVGALQARIDDRFAAA
                     VRLILDSRGRLIVSGMGKSGHIARKIAATMASTGTPAYFVHPAEASHGDLGMITRHDI
                     LLALSNSGESEELLRIL"
BASE COUNT          370 a          608 c          526 g          242 t
ORIGIN      
        1 cgccgccgcc cgccgcctcg cgcacgcggt ccggatcgag gtcgagggcg atgctctgta
       61 tcccttcctg ggccaggaag cgcgccaagt actggccgtt gcgcccgaag ccgcagagga
      121 tcgcgtgctt ctccgaagtc atcgactggg cggcaatctg cgtcacctgc atcgagcgca
      181 gcaaccattc gctggcgacg aaacgcatca ccagacgctc gctgtactgg acgatgaagg
      241 gcgcaatcag catcgacagc accagcgaag tcaacaccac ctgctcgatc cccttgggca
      301 ggtgggtgat cacgccgagc agcacgaaac cgaattcacc gccggcgcac agccacaacc
      361 ccgagcggat ggcattgccg gacgaggcgc cgagcttcca cgacaggagg ccgacgatca
      421 gcgccttgat caccagcagc aggaccagca ccagcatcac ctgccaccac aggccgacca
      481 ggatatggag gtcgagcttg acgccgatgg tgacgaagaa aagccccatc agcacgtcgc
      541 ggaagggctt gatgtcctct tccacctgca tgcggaactc ggtttcggcg atcagcatgc
      601 cggcgacgaa ggcgcccagc gccagcgaca ggccggcgtg ctcggtgatc gtcgccaggc
      661 cgagggtgat gagcagcacg ttgaggacga acacctcggc cgacttggcg cgggcgacga
      721 aatggaacca cttgcgcatc agcttctggc cgaacaccag gatcagcgcc agcaccgccg
      781 ccgccttgag catggcgacg cccatcagca tcgccagctt ttccggcgat tcggtcagcg
      841 acggcagcag gaccagcaac ggcaccacgg cgaggtcctg gaacaacagc acgccgacga
      901 tctgccggcc atgcaccgaa tcgagctgca tgcgttccgc cagcagcttg ctcagcaccg
      961 ccgtggacga catggcgaac accgcgccca gcgcgatccc cagttgccag ccgatgccca
     1021 acgccatcac cagcaagagc acgaccagca tggtcaacag cacctgcagc aagcccagcc
     1081 cgaagacgat gcgtttcatc gcgtagagct tgggcaggga gaattcgagg ccgatcgaga
     1141 acatcaggaa gacgacgccg aattcggcga tgtattcggc gcgatggatg tccgacatca
     1201 gattgaacgc gtgcggcccg atcatgctgc ccaccgccag gtagccaagc accggcggca
     1261 agttgaaacg acggaagatg accacggcga gaaccgaggc tccgagcagc agcaggacga
     1321 gggaaagggc gctcaaatcg ggcttgatga agtcaggtat actccggcca tcataaccgc
     1381 agcccgccca tgaacccaaa tccgtcgccc gcctgttttt cgccggaacg cgccctgctg
     1441 ctgggccggc agacgctcgc catcgaggcg gcggcggtcg gcgcgctgca ggcccggatc
     1501 gacgaccgtt tcgccgccgc cgtccgcctc atcctcgaca gccgcggccg cctgatcgtc
     1561 agcggcatgg ggaaatccgg tcacatcgcg cgcaagatcg ccgccaccat ggccagtacc
     1621 ggcacccctg cctacttcgt ccatccggcc gaagccagcc acggcgacct cggcatgatc
     1681 acccgccacg acatcctgct cgccctctcc aattccggcg aatccgagga gttgctgcgc
     1741 attctg
//
LOCUS       DAOSDX010000681         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23113_length_1744_cov_3.318532, whole genome shotgun sequence.
ACCESSION   DAOSDX010000681 DAOSDX010000000
VERSION     DAOSDX010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23113_length_1744_cov_3.318532"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLN02_13070"
     CDS             <1..870
                     /locus_tag="PLN02_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI53306.1"
                     /translation="RGDLIHNCFSYHFTPGAFLMEGGARKLGCAVFPGGVGQTEQQVQ
                     AMVDLRPDGYVGTPSFLRIIVEKAEELGADISSLKKALVSGEALPGVTRAWLKERGIA
                     VRQCYATADIGAIAYESEAADGMVVEEDLLVEIVRPGTGDPVPVGEVGEVVVTSFNTD
                     YPLIRFATGDLSAILPGISPCGRTNIRLRGWLGRADQTTKIKGMFVHPEQIAEIARRF
                     PEVARMRLVVDNPDAQDRMVLHCETAANPDGLSGKLVESLREQTKLRGSIEIVAPGGL
                     PNDGKVIEDRRVY"
     gene            886..1386
                     /locus_tag="PLN02_13075"
     CDS             886..1386
                     /locus_tag="PLN02_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53307.1"
                     /translation="MRVLALALALLAVPLQAEEASLRPSASLLFKYPELLRPGSCTVY
                     REGGAGWIMVEPMYYLRGTVVSSEVTTRKLQQCPAVPGKTREQYTRQEYVRLVEATPC
                     LAVGEAERDAQVGVVRLRVDDWETPHVRKAENTGRLYRGLFLDRPLQKGMEIELEADL
                     LGPCEP"
     gene            complement(1393..>1744)
                     /locus_tag="PLN02_13080"
     CDS             complement(1393..>1744)
                     /locus_tag="PLN02_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOI53308.1"
                     /translation="WPVMKRRLTEIFRAQPRRHWETLFADSDDCVWPVLALGEAADHP
                     HNRARGAFIELAGVRQPAPAPRLSRTPGAVQSAPPLLGEHSRERLAALGLDDQEIERL
                     AALGAVTLRPSAAD"
BASE COUNT          278 a          595 c          600 g          271 t
ORIGIN      
        1 cgcggcgacc tgatccacaa ctgcttctcc taccacttca cgcccggcgc cttcctcatg
       61 gagggcggcg cccgcaagct cggctgcgcg gtctttccgg gcggcgtcgg gcagaccgag
      121 cagcaggtgc aggcgatggt agacctgcgc ccggacggct atgtcggcac gccgtccttc
      181 ctccggatca tcgtcgagaa agccgaagaa ctgggcgccg acatttcctc gctgaagaag
      241 gcgctggtct ccggcgaagc gctgcccggc gtcacccgcg cctggctgaa ggaacgcggg
      301 atcgccgtcc gccagtgcta cgcgaccgcc gacatcgggg cgatcgccta cgagagcgag
      361 gccgcggacg gcatggtggt cgaggaggac ctgctggtcg agatcgtccg cccggggacc
      421 ggcgatccgg tgccggtcgg cgaagtgggc gaggtggtgg tgaccagctt caacaccgac
      481 tacccgctga tccgcttcgc caccggcgat ctgtcggcga tcctgcccgg catctcgccc
      541 tgcggccgga ccaatatccg cctgcgcggc tggctcggcc gcgccgacca gacgaccaag
      601 atcaagggca tgttcgtcca tcccgagcag attgccgaga tcgcccggcg ctttccggaa
      661 gtcgcccgca tgcgcctggt cgtcgacaac ccggatgccc aggaccgcat ggtcctgcac
      721 tgcgaaacgg cggcaaatcc ggacggcctg agcggaaaac tggtggaaag cctgcgcgag
      781 cagacaaaat tgcgcggttc gatcgaaatt gttgcccccg ggggcttgcc gaacgatgga
      841 aaggtgatcg aagatagacg ggtctactga cctggaatcc cgtcaatgcg cgtacttgct
      901 ctcgccctgg ccctgctggc cgtgccgttg caagccgagg aagcctcgct gcgtccgtct
      961 gccagcctgt tgttcaagta tcccgagctg ctgcgcccgg gcagttgcac ggtgtaccgc
     1021 gagggcggcg ccggctggat catggtcgaa ccgatgtatt acctgcgcgg taccgtcgtc
     1081 agcagcgagg tgacgacgcg caagctgcag cagtgtccgg cggtgccggg caagacccgc
     1141 gaacaataca cgcgccagga gtacgtccgc ctggtcgagg cgacgccctg tctggcggtc
     1201 ggcgaagccg agcgcgacgc gcaggtcggc gtcgtccgcc tgcgcgtcga cgactgggag
     1261 acgccgcacg tgcgcaaggc ggaaaacacc ggccgcctct accgcggcct cttccttgac
     1321 cggcccttgc aaaagggcat ggagatcgag ctcgaggccg acctgctcgg cccctgcgag
     1381 ccctgatccg gttcagtcgg cggccgacgg ccgcagcgtg acggcgccga gcgccgccag
     1441 tctttcgatt tcctgatcgt cgagaccgag cgcggccagc cgttcgcggc tatgttcgcc
     1501 gagcagcggt ggcgccgatt gcacggcgcc cggcgtccgc gacaggcggg gtgccggtgc
     1561 cggttgacgc accccggcca gctcgatgaa cgcaccgcgc gcccggttgt gcgggtggtc
     1621 ggcggcttcg cccagcgcca gcaccggcca cacgcagtcg tcgctgtcgg cgaacagggt
     1681 ttcccagtgg cggcgcggct gggcacggaa gatttccgtc aggcgccgct tcattaccgg
     1741 ccag
//
LOCUS       DAOSDX010000682         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23118_length_1744_cov_2.414446, whole genome shotgun sequence.
ACCESSION   DAOSDX010000682 DAOSDX010000000
VERSION     DAOSDX010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23118_length_1744_cov_2.414446"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLN02_13085"
     CDS             <1..870
                     /locus_tag="PLN02_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOI53309.1"
                     /translation="ELNITTGAVSQQIKLLEDELGIAIFERSGKRFTGITEPGRVVID
                     IARRILREAENLKRASGEFSSGDSGKLVLAATHTQARYALPTVVRDFVARHPKVKLEM
                     HQGNPVQIADWVASGEADFGIATESLGEHPQLVSFPVQQWSHCVIAPAGHAILASQPL
                     ALQELARWPLITYDAAFTGRSRINRAFERLEIEPSSALTALDADVIKTYVSLGLGLGI
                     IAKLAFDPQRDAGLVAIDAGHLFESNTTRLAVRRGSYLRRFDYDFIELFAPHLNKRVV
                     EMTLQGGGEAWQL"
     gene            complement(922..1707)
                     /locus_tag="PLN02_13090"
     CDS             complement(922..1707)
                     /locus_tag="PLN02_13090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI53310.1"
                     /translation="MFKLSSLAALLVAGSLISAPAFAQSKAYATVNGKAIPQTVYDAF
                     LAEQKAQGAPDSPELKNAIREELIRRELLSQEAAKKGLDKKPEVRGQMELARQAVLIR
                     SFMGDYVRSHPVSDAQLKSDYEQLKKSLGSTEYKARHILVEKEDDAKAIIAKLDKGEK
                     FATLAKQSLDPGSKDKGGELEWSAPTAYVKPFGDTLTTLKKGEYTKAPVKTDYGFHVI
                     QLEDSRPLTPPPFEQVKPQLQQRATQMQVESLIKDLRSKAKVN"
BASE COUNT          271 a          572 c          567 g          334 t
ORIGIN      
        1 gaactgaaca tcaccaccgg cgcggtcagc cagcagatca agctgctcga ggacgaactt
       61 ggcatcgcca tcttcgagcg cagcggcaag cgcttcaccg gcatcaccga accgggccgg
      121 gtggtcatcg acatcgcccg ccgcatcctg cgcgaggcgg aaaacctgaa gcgtgccagc
      181 ggcgaattct ccagcggcga ctccggcaag ctggtgctgg cggcaaccca tacccaggcc
      241 cgctacgcgc tgccgaccgt ggtccgcgac ttcgtcgccc gtcaccccaa ggtcaagctc
      301 gagatgcacc agggcaaccc ggtgcagatc gccgactggg tggcctccgg cgaggccgat
      361 ttcgggatcg ccaccgagtc gctcggcgag cacccgcaac tcgtttcctt tccggtccag
      421 cagtggtcgc actgcgtcat cgcgccggcc ggccatgcca tcctggccag ccagccgctg
      481 gcgctgcagg aactggcgcg ctggccgctg atcacctacg acgcggcgtt caccggccgc
      541 tcgcggatca accgtgcctt cgaacgcctg gagatcgaac ccagcagcgc gctcaccgcg
      601 ctcgacgccg acgtgatcaa gacctacgtc agcctcggcc tcggcctcgg catcatcgcc
      661 aagctcgcct tcgatcccca gcgcgacgca ggcctggtgg cgatcgacgc cggccacctg
      721 ttcgaatcga acaccacccg gctggcggtg cggcgtggca gctacctgcg ccgcttcgac
      781 tacgacttca tcgaactgtt cgccccccac ctcaacaagc gcgtcgtcga gatgaccctg
      841 cagggcggtg gcgaggcctg gcagctctag cagcaaaaaa aagcccgccg gctggcgggc
      901 tggtacggtg ttcgggtgag gtcagttgac cttggccttc gaccgcaggt ccttgatcag
      961 gctttccacc tgcatctgcg tcgcgcgttg ctgcagttgc ggcttgacct gttcgaacgg
     1021 cggcggcgtc agcgggcggc tgtcttcgag ctggatgacg tggaaaccgt agtcggtctt
     1081 caccggcgcc ttggtgtatt cgcccttctt cagcgtcgtc agggtgtcgc cgaaaggctt
     1141 gacgtaggcg gtcggggcgc tccattcgag ttcgccgccc ttgtccttgg agcccgggtc
     1201 cagcgactgc ttggccaggg tggcgaattt ctcgcccttg tccagcttgg cgatgatcgc
     1261 cttggcgtcg tcttcctttt cgaccaggat gtggcgcgcc ttgtactcgg tgctgccgag
     1321 cgacttcttc aactgctcgt aatcggattt cagctgggcg tcgctgaccg gatggctgcg
     1381 cacgtagtcg cccatgaagg agcggatcag cacggcctga cgggccagtt ccatctggcc
     1441 gcggacttcc ggcttcttgt cgaggccctt cttggcggct tcctgcgaga gcagttcgcg
     1501 gcggatcagt tcttccctga tcgcgttctt gagttcggga gaatccggag cgccctgcgc
     1561 cttttgctcg gcaaggaagg cgtcgtacac ggtttgcggg atggccttgc cgttgaccgt
     1621 ggcgtaggcc ttgctctggg cgaaggcggg tgcggagatg aggctgccgg caaccagcag
     1681 tgcagccagg gagctgagtt tgaacatggg gatggggtcc tgggggtggt tggtacgaaa
     1741 gtca
//
LOCUS       DAOSDX010000683         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23169_length_1741_cov_3.078292, whole genome shotgun sequence.
ACCESSION   DAOSDX010000683 DAOSDX010000000
VERSION     DAOSDX010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23169_length_1741_cov_3.078292"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLN02_13095"
     CDS             complement(<1..722)
                     /locus_tag="PLN02_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HOI53311.1"
                     /translation="MRQYAKPTLPPLPVEPDGLAPPSARLQHQGQSQALTASRWPARL
                     DLLQGISGLALALFLVAHMAFVASILVSEEMFYRVARFFEGEWLFGTPHPWLVSLVAA
                     GVTGLLIVHAWLALRKFPASYRQHSAFSAHRRRLRHVDTSLWWLQVVSGFILFFLTTI
                     HLYDMLSQPALIGPYESADRVWSGRMWPIYLALLFTAEVHGGIGLYRLALKWGWFVGD
                     DPNRGRLRLRRVRHVFTAFFVTL"
     gene            971..>1741
                     /locus_tag="PLN02_13100"
     CDS             971..>1741
                     /locus_tag="PLN02_13100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOI53312.1"
                     /translation="MSTLQEIFAPDGPFARAIDGYRVRPQQVEMAERIAAAIKDNAVF
                     IAEAGTGTGKTFAYLVPALKSHGKVIVSTGTKTLQDQLYNKDLPMVREILKAPVRTAL
                     LKGRANYVCPYHLNRSLADGRFLSREDAADARRIAAFAKITQTGDKAECNEVNENSPV
                     WNHATSTRDNCLGQECPDYKTCFVMQARKEAMAADLVVVNHHLFFADVMLRDEGMAEL
                     LPACNTVIFDEAHQLPEVATLFFGDSLSTAQVLDLCRDT"
BASE COUNT          330 a          553 c          575 g          283 t
ORIGIN      
        1 agggtgacga agaaggcggt aaacacgtgg cgcacccggc gcaggcgcag tcggccgcgg
       61 ttcgggtcgt cgccgacaaa ccagccccac ttcagcgcca ggcggtagag gccgatgccg
      121 ccatggactt cggcggtgaa cagcagggcc aggtagatcg gccacatgcg cccgctccag
      181 acgcggtccg ccgattcgta ggggccgatc agcgccggct ggctgagcat gtcgtagagg
      241 tggatggtgg tcaggaagaa gaggatgaaa ccgcttacca cctgcagcca ccacaggctg
      301 gtatcgacgt ggcgcaggcg tcggcggtgg gcactaaagg cgctgtgctg gcggtagctg
      361 gcgggaaact tgcgcagcgc cagccaggcg tggacgatca gcaagccggt gacgccggcg
      421 gcgaccagcg aaaccagcca cggatgcggc gtgccgaaca gccattcgcc ttcgaagaag
      481 cgggcgacgc ggtagaacat ttcctcgctg accaggatgc tggcgacgaa agccatgtgg
      541 gcaaccagaa agagcgccag ggcgaggccg gaaatcccct gcagcagatc gagccgggcc
      601 ggccagcggc tggcggtcag agcttggctc tggccctggt gttgcagcct ggcgctgggc
      661 ggggcgaggc cgtccggttc gaccggcagc ggcggtaagg tgggcttggc gtattggcgc
      721 atggctgact tcctctcgtt gggctcgcgc tgcttgtcgt tatgagccaa atctagcatg
      781 aaaaagcgtg agtttcgcca aaatattgtg cgctgcagca agtgcaataa tctggattat
      841 cgaatgtttg cggattgttt cgcgaaacat tgctgcaatg caccacagcg gggcgaagtc
      901 gccggctgtg gcttctgtta agatgccgcg ggccgcagcc gcttcttttt tcgttcgccc
      961 gaccatcgcc ttgtctaccc tccaggaaat cttcgctccc gacggcccgt ttgcccgcgc
     1021 catcgacggc tatcgcgtgc gcccgcagca ggtcgagatg gccgagcgca tcgcggcggc
     1081 gatcaaggac aacgcagtgt tcatcgccga ggccgggacc ggcaccggca agaccttcgc
     1141 ctacctggtg ccggcgctca aatcgcacgg caaggtgatc gtctccaccg gcaccaagac
     1201 gctgcaggac cagctctaca acaaggatct gccgatggtg cgcgagatcc tcaaggcgcc
     1261 ggtgcgtacc gcgctgttga aggggcgggc caactacgtc tgtccctacc acctcaaccg
     1321 gtcgcttgcc gacggccgtt tcctgagccg cgaggacgcc gccgacgcgc gccgcatcgc
     1381 cgccttcgcc aagatcacgc agaccggcga caaggccgaa tgcaacgagg tcaacgagaa
     1441 ctcgccggtg tggaaccacg ccacctcgac gcgcgacaac tgtctcggcc aggagtgccc
     1501 ggactacaag acctgcttcg tcatgcaggc gcgcaaggag gcgatggccg ccgacctggt
     1561 cgtcgtcaat caccatctct tcttcgccga cgtcatgctg cgcgacgaag gcatggccga
     1621 actactgccc gcctgcaaca cggtgatctt cgacgaggcg caccagttgc cggaagtggc
     1681 gacgctgttc ttcggcgaca gcctgtccac cgcgcaggtg ctcgacctct gccgcgacac
     1741 g
//
LOCUS       DAOSDX010000684         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23289_length_1733_cov_3.715733, whole genome shotgun sequence.
ACCESSION   DAOSDX010000684 DAOSDX010000000
VERSION     DAOSDX010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23289_length_1733_cov_3.715733"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLN02_13105"
     CDS             complement(<1..272)
                     /locus_tag="PLN02_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HOI53313.1"
                     /translation="MTPPFPSRPRPIAGALRAALLGLALAAPLTLATIAPAQAQSAAR
                     SYQIAAGRLDATLTAFAAAADITLSFDAAQTRGLRSAGLNGAYTTAG"
     gene            complement(383..1324)
                     /locus_tag="PLN02_13110"
     CDS             complement(383..1324)
                     /locus_tag="PLN02_13110"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOI53314.1"
                     /translation="MAGRELPRATLEAAAHWYVQLNDATTAAAERAAWERWLAADASH
                     RQAWARMQALSQRLHAVPGELAGPTLSGARERRRRTLKLLALLLSGGGTLALLPELEA
                     GWQVAAADLRTATGERRRVVLADGGSLDLNSASAVDVDYGANLRRLRLLAGEIFVSTA
                     ADPRPFEVHTGHGAIRALGTRFGVRLDGATTRVAVHQHAVEIRPLDGPPRRLAAGEEV
                     DFDAAGVSPSQPLTAGSGAWRDGRLIAIDRRLDDFLGELARHRRGHLAWSPAVAGLRL
                     SGVFRLADSDAVLDNLAASLPIRLHFLTRYWVRVDAA"
     gene            complement(1326..>1733)
                     /locus_tag="PLN02_13115"
     CDS             complement(1326..>1733)
                     /locus_tag="PLN02_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOI53315.1"
                     /translation="DAADLAHDTFLRIIAARNAAAIEEPRKYLATIAKGLVVDRFRRQ
                     SIEAAWLETLAARPEPLAISPETRALILETLLAIDRMLDELGPRAREIFLLAQFEGLT
                     YAAIGERLGVSVTTVKKHLARALAHCLILAEGC"
BASE COUNT          241 a          562 c          699 g          231 t
ORIGIN      
        1 ccggcggtgg tgtaggcgcc gttgaggccg gcgctgcgca ggccgcgcgt ctgggcggcg
       61 tcgaacgaga gggtgatgtc ggcggcggca gcgaaggcgg tgagcgtcgc gtcgagccgg
      121 ccggcggcga tctggtagct gcgcgcggcg ctctgcgctt gcgccggcgc gatcgtcgcc
      181 agcgtcagcg gggcggccag ggccaggccg agcagggcgg cgcgcagggc gccggcgatg
      241 ggtcgcgggc gcgacgggaa gggtggcgtc atggtgaaaa ttcctctttt ttccgggttg
      301 accgtggacg ggcgcgggcg cgcctctcta ctggttggcc ggatggcgga aaaaaagagc
      361 aaccccggga tggaaataat tctcaagcgg cgtcgacgcg aacccagtaa cgggtcagga
      421 aatgcagccg gatcggcagc gatgccgcca ggttgtcgag cacggcgtcg ctgtcggcca
      481 gacggaagac gccggacagg cgcagtccgg cgaccgcggg cgaccaggcg agatggccgc
      541 ggcggtggcg ggcgagttcg ccgaggaaat cgtcgaggcg gcggtcgatg gcgatcaggc
      601 ggccgtcgcg ccaggcgccg ctgccggccg tcaggggctg gctcgggctg acgccggcgg
      661 cgtcgaaatc gacctcttcg cccgccgcca gccggcgcgg cgggccgtcg agcgggcgaa
      721 tctcgaccgc gtgctggtgc accgccaccc gcgtcgtcgc gccgtcgagg cgaacgccga
      781 aacgggtgcc gagggcgcgg atcgcgccgt gcccggtatg cacttcgaag gggcgcggat
      841 cggcggcggt gctgacgaag atctcgccgg cgagcaggcg cagccggcgc aaattggcac
      901 cgtaatcgac gtcgaccgcg gaagcgctgt tgaggtcgag gctgccgccg tcggcgagga
      961 cgacgcggcg ccgctcgccg gtggcggtac gcaggtcggc ggcggcgacc tgccagccgg
     1021 cctcgagttc gggcagcagg gcgagcgtgc cgccgcccga gagcagcaac gcgagcaatt
     1081 tgagcgtgcg ccggcggcgc tcgcgggcac cgctcagcgt cggcccggca agctcgccgg
     1141 gaaccgcgtg cagccgctgg ctgagcgcct gcatccgcgc ccacgcctgg cggtggctgg
     1201 cgtcggcggc gagccagcgt tcccaggcgg cgcgctcggc ggccgcggtc gtcgcgtcgt
     1261 tcaactgcac gtaccagtgc gcagcggcct ccagcgttgc ccgcggcagt tcgcgaccgg
     1321 ccatctcagc agccctcggc aaggatcagg cagtgcgcca gcgcccgcgc caggtgcttc
     1381 ttgacggtcg tcaccgaaac gccgaggcgt tcgccgatgg cggcgtaggt gaggccctcg
     1441 aactgggcaa ggaggaagat ctcgcgggcg cgcgggccga gttcgtcgag catgcggtcg
     1501 atggcgagca gggtttccag gatcagggcc cgtgtctcgg gcgaaatggc cagcggctcc
     1561 ggccgcgccg ccagcgtttc cagccaggcg gcctcgatgc tctggcggcg gaagcggtcg
     1621 accaccaggc ccttggcgat cgtcgccagg tatttgcgtg gttcctcgat tgccgccgcg
     1681 ttgcgtgccg cgatgatgcg caggaaggtg tcgtgggcga gatcggcggc gtc
//
LOCUS       DAOSDX010000685         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23393_length_1726_cov_2.811490, whole genome shotgun sequence.
ACCESSION   DAOSDX010000685 DAOSDX010000000
VERSION     DAOSDX010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23393_length_1726_cov_2.811490"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLN02_13120"
     CDS             <1..427
                     /locus_tag="PLN02_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53316.1"
                     /translation="GRWLRFLLLTAFCLGSSVATAELVVVVNARSGVAAMTRNEVINI
                     FFGRNRQFFNGTEVQPVDLVDANPQRAQFYRLLVGKDLSEINAYWSRQIFTGRMQAPP
                     RVDSSEDVLKWVAARPGGIGFVELSRADARVRVVYELKP"
     gene            540..980
                     /locus_tag="PLN02_13125"
     CDS             540..980
                     /locus_tag="PLN02_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOI53317.1"
                     /translation="MTARPLLEIIADRPLVTATPQTTVRAACQLMASKRIGALLIVDG
                     GRTVGIFTERDALNKVLAGALDPDSTPLSAVMVRDPQCVRADRPLAHALLLMAEGGFR
                     HVPVVDADGRAIGMVSARDALGRDLVQLEHDIERLEALENSIGY"
     gene            complement(974..1705)
                     /locus_tag="PLN02_13130"
     CDS             complement(974..1705)
                     /locus_tag="PLN02_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HOI53318.1"
                     /translation="MPEQLPILFRDEHIVVIDKPPNLLVHRSEIDRHETRFAIQLLRD
                     QIGQKVWPAHRLDRGTSGVLLFGLSAEIASALGRQFAEGTVAKRYLAVVRGHPPASGS
                     IDHALSRQRDDYEFQGERSSQEAQSALTHYRRLATIELPVAVDRYPSSRYALLELTPV
                     TGRKHQLRRHLKHIAHPIIGDATYGKGRHNRYFADEFDCHRLLLACTRLAFRHPVTQL
                     AIAIDAPPGGEFAALLGRFGWSEAQ"
BASE COUNT          286 a          520 c          612 g          308 t
ORIGIN      
        1 tggacgctgg ctgagattcc tgctgctgac tgccttttgc ctgggcagca gcgtggcgac
       61 cgccgaactg gtcgtcgtcg tcaatgcgcg cagtggggtg gcggcgatga cgcgcaacga
      121 agtgatcaac atctttttcg ggcgcaatcg ccagttcttc aatggcactg aggtgcagcc
      181 ggtcgacctg gtcgacgcca acccgcagcg ggcgcagttc taccgcttgc tggtcggcaa
      241 ggatctttcc gaaatcaacg cctactggtc gcggcagatt tttaccgggc gcatgcaggc
      301 gccgccgcgc gtcgatagct ccgaagatgt cctcaaatgg gtggcggcgc gcccgggcgg
      361 cattggtttc gtcgagttga gccgggccga cgcccgcgtc cgcgtggtct acgaactgaa
      421 gccgtgagcg gcttgcggtc ggcttcataa aatctcaata tggcagcggc agactggcct
      481 tggccaggcg ccggccggcg acataacgtc ccggacccat aacgatgagg agacccgaaa
      541 tgactgcacg ccccttgctg gaaatcatcg ccgaccgtcc gctggtgacg gcaacgccgc
      601 aaactaccgt acgcgccgcc tgccagttga tggcgagtaa acggatcggt gccctgctga
      661 tcgtcgacgg tgggcggacc gtcggcatct tcaccgagcg cgatgcgctc aacaaggtgc
      721 tggccggcgc gctcgacccc gacagcacac cgctctcggc ggtgatggtg cgcgacccgc
      781 aatgtgtccg ggccgaccgg ccgctggccc atgccctgtt gctgatggcc gaaggcggtt
      841 tccggcacgt gccggtggtc gatgccgacg ggcgggcgat cggcatggtg tcggcacgcg
      901 acgcgctggg tcgtgacctg gtgcagctcg aacacgacat cgagcgcctg gaagcgttgg
      961 aaaactcgat cggctactga gcttccgacc agccgaagcg cccgagcagg gcggcgaatt
     1021 cgccgcccgg tggggcgtcg atcgcgatcg ccagttgtgt gaccgggtgt cggaaggcga
     1081 ggcgggtgca cgccagcagc agccggtggc agtcgaactc gtcggcgaaa taacggttgt
     1141 ggcggccctt gccgtaggtc gcatcgccga tgatcggatg ggcgatgtgc ttgaggtggc
     1201 ggcgcaactg gtgcttgcgg ccggtgaccg gggtgagttc gagcagcgcg tagcgactgc
     1261 tcgggtagcg gtcgacggcg accggcagtt cgatcgtcgc cagccgccgg tagtgggtca
     1321 gcgccgactg cgcttcctga ctgctgcgct cgccctggaa ttcgtaatcg tcgcgctgcc
     1381 ggctcagcgc gtgatcgatg ctgccacttg ccggcggatg gccgcgcacg acggcgaggt
     1441 aacgcttggc gaccgtgcct tcggcaaatt gccggccgag cgcgctggcg atctctgcag
     1501 aaagaccaaa gagcaggacg cccgaagtgc cgcgatcgag tctatgcgcc ggccagacct
     1561 tctgcccgat ctggtcgcgt agtagctgga tcgcgaagcg cgtctcgtgg cggtcgattt
     1621 ccgagcgatg gacgagcagg ttgggcggct tgtcgatgac gacgatgtgc tcgtcgcgga
     1681 aaaggatggg tagctgttca ggcaaagaag cgttgccggg cggtat
//
LOCUS       DAOSDX010000686         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23416_length_1725_cov_2.801796, whole genome shotgun sequence.
ACCESSION   DAOSDX010000686 DAOSDX010000000
VERSION     DAOSDX010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23416_length_1725_cov_2.801796"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLN02_13135"
     CDS             complement(<1..987)
                     /locus_tag="PLN02_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53319.1"
                     /translation="MPLIAPSADSREYLLNLRRLFALRWAMLAAMALLVVVAPPLIGI
                     ELPRLVMFAIILVVALLNGFGQWQAARSRDASANELCSQLLIDIVALSALMFFSGGAT
                     NPLVSLLLPPVAIAALGLPQRLVLLVGSTAVVAYSLLMLYYLPLPIADAARATQLHLI
                     GMWLTFTVSALLIAWIILRMTRLIRQRDAELATAREQALRDERVTAMGTLAAGAAHEL
                     GTPLATMTLIVGELAGDERLPDDARDDLGLLRQQVAACKTIITGLSRRAGAERLEDTH
                     AQAVDQWLDELRCQWQALRPAASSRLQVLSQDAAPRLAIDPRLEQALLNLLNN"
     gene            1132..>1725
                     /locus_tag="PLN02_13140"
     CDS             1132..>1725
                     /locus_tag="PLN02_13140"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HOI53320.1"
                     /translation="MPFALRDNFLLALFYALTGWLALQIAVPPGYAAPLFPPAGVALA
                     ALLVRGPRCLPGVFVGAFVVQSLAAMQSGLSTAVWPMLVVPALGATAQAAFGYLLAGR
                     LLGRDNPLDSPDSIVRFVFVVAPLSSLISPTLGTATLYLMQATAFADLPFTWANWWAG
                     DLLGILIMVPLLLVFLGHPADAWRSRRTAVSVPMLIAL"
BASE COUNT          238 a          566 c          612 g          309 t
ORIGIN      
        1 gttgttcagc aggttgagca gcgcctgttc gaggcgcggg tcgatcgcca ggcgcggcgc
       61 cgcgtcctgg ctgagcacct gcaggcggct gctggcggcc gggcgcaggg cttgccactg
      121 gcagcgcagt tcgtcgagcc attggtcgac cgcctgggca tgcgtgtctt ccaggcgttc
      181 ggcgccggcc cgccgcgaca ggccggtgat gatcgtcttg caggcggcga cctgctggcg
      241 cagcaggccg aggtcgtcgc gggcgtcgtc aggcagacgt tcgtcgccgg ccagttcgcc
      301 gacgatcagc gtcatcgtcg ccagcggagt gcccagttcg tgggcggcgc cggcggccag
      361 tgtgcccatc gccgtcaccc gttcgtcgcg cagcgcctgc tcgcgcgcgg tggcgagttc
      421 ggcgtcgcgc tggcggatca gccgggtcat gcgcaggatg atccaggcga tcagcagcgc
      481 cgagacggtg aaggtcagcc acatgccgat caggtgcagt tgcgtcgccc gcgccgcgtc
      541 ggcgatcggc agcggcaggt agtagagcat gagcagcgaa taggcgacga ccgccgtgct
      601 gccgaccagc aataccaggc gctgcggcag gccgagggcg gcgatggcga ccggcggcag
      661 cagcagcgag accagcgggt tggtggcgcc gccgctgaag aacatcagcg cgctcagggc
      721 gacgatgtcg atcagcaact ggctgcacag ttcgttggcg ctggcgtccc ggctgcgcgc
      781 cgcctgccac tggccgaagc cgttgagcag ggcgacgacg aggatgatcg cgaacatcac
      841 caggcgcggc agctcaatgc cgatcagtgg cggggcaacc accaccagca gcgccatcgc
      901 cgccagcatc gcccagcgca gggcgaacag tcggcgcagg ttgagcaggt attccctgct
      961 gtcggcggag ggggcgatca gtggcatgcg cggattatcg gcgatttgcc cggtgcggtg
     1021 cgtagccaca ggctgcggcg gattgtcgca gtctgtggcg tcccggcgga ctgccttttg
     1081 cggtgaagcc tacttataat gggaaaatta tcagagaaat aaaatataaa aatgcctttt
     1141 gcgttgcgcg acaacttcct gctcgccctg ttttatgcgt tgaccggctg gctggccttg
     1201 cagattgccg tgccgccggg ttatgcagcc cctcttttcc cgccagccgg ggtcgcgctg
     1261 gcggcgctgc tggtgcgcgg gccgcgctgt ctgcccggcg tcttcgtcgg cgctttcgtc
     1321 gtccagtcgc tggcggcgat gcaaagcggc ttgtcgacgg cggtctggcc gatgcttgtc
     1381 gtgccggcgc tcggtgccac cgcgcaagcc gccttcggtt acctgctcgc cggtcgtctg
     1441 ctcgggcgcg acaacccgct cgacagcccg gacagcatcg tccgtttcgt cttcgtcgtc
     1501 gccccgctgt cgtcgctgat cagtccgacg ctgggtacgg cgacgctcta cctgatgcag
     1561 gcgaccgcgt tcgccgattt gcccttcacc tgggccaact ggtgggccgg cgacctgctc
     1621 ggcatcctca tcatggtgcc gctgctgctc gtcttcctcg gtcatccggc cgatgcctgg
     1681 cgctcgcggc ggaccgccgt cagcgtgccg atgctgatcg cgctg
//
LOCUS       DAOSDX010000687         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23429_length_1724_cov_3.199521, whole genome shotgun sequence.
ACCESSION   DAOSDX010000687 DAOSDX010000000
VERSION     DAOSDX010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23429_length_1724_cov_3.199521"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1006)
                     /locus_tag="PLN02_13145"
     CDS             complement(167..1006)
                     /locus_tag="PLN02_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI53321.1"
                     /translation="MATTAFTALACPLDGAPLTPVDGGWTCPAGHRYDQAAQGYVNLL
                     PVQHKRSRDPGDSKEMVAARRRFLEAGHYRLIADLAARAALADLPADAACLDAGCGEG
                     YYLRQLAAAAEAAGKTPALLGLDISKWAVQAAAKAQRGTATWVVGSNANLPVLPATLD
                     RVLCLFGFPVYPEFARVLKPGGQLIMIDAGPDHLRELREIIYPTLKTPAPPNDQAPPG
                     FSLQQREALSCRIELASQEQIADLLVMTPHFYRASPAGRERAAQLSRLELTVDVRLQI
                     FGT"
     gene            complement(1015..>1724)
                     /locus_tag="PLN02_13150"
     CDS             complement(1015..>1724)
                     /locus_tag="PLN02_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI53322.1"
                     /translation="PPPADAPAGTPPTTIVRGINRSVMAGQRIGILGANGQGKSTLVK
                     TIARDLGAISGQVTEGKGLNIGYFAQQELDVLRPQDTPLEHMIRLAKEAIAAGRSNVP
                     GREQELRNFLGNFNFGGEMVKQAVGTMSGGEKARLVLCMLVWQRPNLLLLDEPTNHLD
                     LNTREALSVALNEFEGTVMLVSHDRAMLRSVCDEFWLVSRGGVGPFDGDLDDYQRYLL
                     DEAKRAREEASALLKAA"
BASE COUNT          243 a          545 c          633 g          303 t
ORIGIN      
        1 atggcatcga tcgcagtccc tggcaggcca gtggcggtct gtcgctggtg catcggcaca
       61 agagcggggc ggaaatcaag ctgcgttatg acgccgaatc gcgtcgcgct tacctgtctc
      121 atgcggcgtc ggccgttgtg cgctggactt tctagcaacg caccggtcag gtgccgaaaa
      181 tctgcagccg cacatcgacc gtcagttcca gccggctgag ttgcgccgcc ctttcccggc
      241 cggccgggct ggcgcggtag aagtgcgggg tcatcaccag caggtcggcg atttgttcct
      301 ggctggcgag ttcgatgcgg caggacagcg cttcgcgctg ctgcaaggaa aatcccggtg
      361 gtgcctgatc gttcggcggg gccggggttt tcagcgtcgg gtagatgatt tcgcgcagtt
      421 cgcgcaggtg atccgggccg gcgtcgatca tgatcagttg gccgcccggc ttgagcacgc
      481 gggcgaattc cgggtagacc ggaaaaccga acagacacag cacgcggtcc agcgtcgccg
      541 gcagtaccgg cagattggcg ttgctgccca ccacccaggt tgcggtgccg cgctgcgctt
      601 tggccgccgc ctggacggcc catttcgaga tatcgaggcc gagcagcgcc ggggtttttc
      661 cggcggcttc ggctgccgcg gcgagctggc gcaggtagta gccttcgccg caaccggcgt
      721 cgagacaggc ggcgtcggcc ggcaggtcgg ccaaggcggc gcgcgccgcg aggtcggcga
      781 tcagccggta atggccggct tccaggaagc gacggcgggc agcgaccatt tccttgctgt
      841 cgccgggatc acgcgagcgt ttgtgctgta ccggcagcag attgacgtag ccttgcgcgg
      901 cctggtcgta gcggtggccg gccgggcagg tccagccgcc gtcgaccggc gtcagcggcg
      961 cgccgtcgag cgggcaggcg agggcggtga acgctgtcgt cgccatcgcc ggcctcaggc
     1021 cgccttgagc agtgccgaag cttcctcgcg ggcgcgcttg gcttcgtcga gcaggtagcg
     1081 ctggtagtcg tcgaggtcgc cgtcgaaggg gccgacgccg ccgcgcgaga ccagccagaa
     1141 ctcgtcgcag accgagcgca gcatggcgcg gtcgtggctg accagcatca ccgtgccttc
     1201 gaactcgttg agcgcgacgg aaagcgcttc gcgcgtgttg aggtcgaggt ggttggtcgg
     1261 ttcgtcgagg agcagcaggt tgggccgctg ccagaccagc atgcacagca ccaggcgcgc
     1321 cttctcgccg ccgctcatgg tgccgaccgc ctgcttgacc atctcgccgc cgaaattgaa
     1381 gttgccgagg aagttgcgca attcctgctc gcggccgggg acgttgctgc gaccggcggc
     1441 gatcgcttcc ttggccagcc ggatcatgtg ttcgagcggc gtgtcctgcg ggcgcagcac
     1501 gtcgagttcc tgctgcgcga agtagccgat gttcagcccc ttgccttcgg tgacctggcc
     1561 gctgatggcg ccgaggtcgc gggcgatggt cttgaccagc gtcgatttgc cctggccgtt
     1621 ggcgccgagg atgccgatgc gctgaccggc catcaccgaa cggttgatgc cgcgcacgat
     1681 ggtcgtcggc ggcgtgccgg ccggcgcgtc ggcgggcggc gggt
//
LOCUS       DAOSDX010000688         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23434_length_1724_cov_2.444578, whole genome shotgun sequence.
ACCESSION   DAOSDX010000688 DAOSDX010000000
VERSION     DAOSDX010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23434_length_1724_cov_2.444578"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLN02_13155"
     CDS             <1..886
                     /locus_tag="PLN02_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOI53323.1"
                     /translation="RPLLQTYAAIIRPASEELNKTWVAQVQEPFSRKLAIKYPFSPQA
                     GIEAGPAEIAQIFGPEGAVAKFAESALGGLVERRGNTITPRTWGDLGLALQADFMNGF
                     SRWVAPLEGGVAADNQGASQAQTVFMLRPQPAAGTIAYVVEVDGQKLNYRNGASQWAN
                     FIWPNPAGTPGARIMATTFDGETIDIINFPGRFGLEKMINSAARTRQPDGSFRLKWAN
                     RGVEVSIDLRLLSSPQAQSGRSNDGGGERSWNGMSLPLAIAQVEPAAAPVASEPAATS
                     PAAPPPTSPAPATAGGAQ"
     gene            883..>1724
                     /gene="tagF"
                     /locus_tag="PLN02_13160"
     CDS             883..>1724
                     /gene="tagF"
                     /locus_tag="PLN02_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HOI53324.1"
                     /translation="MNTGGKGAVCFGKIPSRGDFIKGGGQHQLIQVLDKWVTQAMEQL
                     SEDPRWKTAYDNAVGIDFVFAGARSRISVVGHIRPSGDASGRRFPFITAGTIERDDAL
                     VFRCAPVALVQIFGELAGVCEAGLQGAEVASLLATLEGIDAAGEFARSLQSDPLGNFV
                     RRTTLEILAAMLGSPDCSAVRRTILAIGLLMRPLLGQGNAVVDKELVFALPRDERNRN
                     LVAGLWIYLVTAFLRKSGVEAQFLIQRAPQAPRLVIGFNGSSAAALAAAIAPESGSER
                     VISLV"
BASE COUNT          287 a          558 c          588 g          291 t
ORIGIN      
        1 gcggcccctg ctgcagacct acgcggcaat catccggccg gcgtcggaag aactgaacaa
       61 gacctgggtc gcccaggtcc aggaaccgtt cagccgcaag ctggcgatca agtatccgtt
      121 ctcgccgcaa gcggggatcg aggctggtcc ggcggaaatc gcccagattt tcgggccgga
      181 aggggcggtc gcgaagttcg ccgaaagcgc gctcggcggt ctggtcgagc gccgaggcaa
      241 cacgatcacg ccgcgtacct ggggcgacct cggtctggcg ctgcaggcgg atttcatgaa
      301 cggcttctcg cgctgggtgg caccgctgga aggtggcgtc gcggccgaca accagggcgc
      361 cagccaggcg cagaccgtct tcatgctgcg cccgcagccg gcggcgggga cgattgcgta
      421 tgtcgtcgaa gtcgacgggc agaaactcaa ctatcgcaac ggcgcgtcgc agtgggccaa
      481 cttcatctgg cccaatccgg cgggaacgcc cggcgcgcgc atcatggcca ccaccttcga
      541 cggcgagacg atcgacatca tcaattttcc ggggcgtttc ggcctggaga agatgatcaa
      601 ctcggctgcg cggacccgcc agcccgatgg ttccttccgc ctgaagtggg ccaaccgcgg
      661 ggtcgaggta agcattgatc tgcgcctgct gagcagcccg caggcgcagt cggggcgctc
      721 gaacgatgga ggaggggagc gcagctggaa cggcatgagc ctgccgctgg cgatcgccca
      781 ggtcgagccg gctgctgcgc ccgtggcaag cgagccggct gcgacttcgc cggcggcgcc
      841 gcctcccacc tcaccagcgc ctgccacggc aggaggtgcg caatgaatac tggtggcaag
      901 ggcgcggtct gcttcggcaa gattccgtca cgcggcgatt tcatcaaggg cggtggccag
      961 caccagttga tccaggtgct cgacaaatgg gtcacccagg cgatggagca attgtccgag
     1021 gacccgcgct ggaaaaccgc ctacgacaac gcggtcggca tcgatttcgt cttcgccgga
     1081 gcgcgcagcc ggatttccgt ggtcggtcac atccggccga gcggcgatgc ctcgggccgg
     1141 cgttttccct tcatcacagc cggcaccatc gagcgcgacg atgcgctggt cttccgttgc
     1201 gcgccggtgg cgctggtgca gattttcggc gaactggccg gcgtctgcga agccggcctg
     1261 caaggcgccg aagttgccag cctgctggca acgctggaag gtatcgatgc ggccggcgag
     1321 ttcgcccgat cattgcaaag cgatcccctg gggaacttcg tgcgccgcac gacccttgaa
     1381 atcctggcgg caatgcttgg ctctcccgac tgctcggcag tacgccggac catccttgcc
     1441 atcggcctgc tgatgcgtcc cttgctgggg cagggcaatg ccgtggtcga caaggaactg
     1501 gttttcgcct tgcccaggga cgagcgcaac cgcaatctgg tcgccggctt gtggatctac
     1561 ctggtgaccg ctttcctgcg caaatccggt gtcgaagcgc agttcctcat tcagcgggcg
     1621 ccacaggccc cgcggctggt catcggtttc aacggcagct cggccgcggc cctggctgct
     1681 gcgatagcgc cggagtcggg cagcgagcgg gtgatttccc tggt
//
LOCUS       DAOSDX010000689         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23482_length_1721_cov_3.475390, whole genome shotgun sequence.
ACCESSION   DAOSDX010000689 DAOSDX010000000
VERSION     DAOSDX010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23482_length_1721_cov_3.475390"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..565)
                     /locus_tag="PLN02_13165"
     CDS             complement(44..565)
                     /locus_tag="PLN02_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HOI53325.1"
                     /translation="MKLLAAALLSLGLAACATPGVEHYRAEQPRLVLEQYFSGKLDAW
                     GMFQGRDGAVQKRFHVVIDASWQGDTGVLDEHFTWSDGTTSRRVWTLRRQPDGRYIGT
                     ADDVVGEAIGEVSGNALRWRYVLALPVDGKVYHVDFDDWMFLVDDKVMLNRSRMSKWG
                     FHLGEVTLSFTKR"
     gene            complement(562..>1721)
                     /locus_tag="PLN02_13170"
     CDS             complement(562..>1721)
                     /locus_tag="PLN02_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOI53326.1"
                     /translation="LYVHVPAFYANVTGLDLGLLGAILLLARLLDAGIDPWLGWLADR
                     VSRRRMVAVSLLPFAVGFVALLNPPVEHAAPWLLASLLLTYAGFSAATVAYQAWGAAV
                     GSDAPERTRLVASREAFGLLGVLVAAALPALLAAALNDGIARLAWVLPVLLVLAGGVT
                     LARVGEGDPRVAVSSALLPALRAVLRDTAFRRLLLVFICNSFAAAIPATLFLFFVADI
                     LQAESASGPLLAAYFLAGAASLPLWLKLSVRIGRPRAWLLAMGVAMLAFAGAGLLGPG
                     DVALFAVVCVASGLALGADLALPAALAADLGERQGRSGACFGVWNFFAKLNLALAAGI
                     ALPLLGVFDYRPGSDQGQTALVFAYALLPLLFKALADFLLWRWRNSLEVES"
BASE COUNT          340 a          592 c          601 g          188 t
ORIGIN      
        1 cccgaccagt ccttgatccg cggattgcct gccatgtcgc cgctcaacgc ttggtgaaac
       61 tgagggtgac ttcgccgagg tggaagcccc acttcgacat gcgcgagcgg ttaagcatca
      121 ccttgtcgtc gaccaggaac atccagtcgt cgaaatcgac gtggtacacc ttgccgtcga
      181 ccggcaacgc cagcacgtag cgccagcgca gcgcattgcc cgagacttcg ccgatggcct
      241 cgccaaccac gtcgtcggcg gtgccgatgt agcgcccgtc gggctgccgg cgcaaggtcc
      301 acacccgacg tgaagtggtg ccgtcggacc aggtgaaatg ctcgtcgagc acaccggtgt
      361 cgccctgcca gctggcgtcg atgacgacgt ggaaacgctt ctgcacggcg ccgtcgcggc
      421 cctggaacat gccccaggcg tcgagcttgc cggagaaata ctgttcgagc accaggcgcg
      481 gttgctcggc ccggtagtgt tcgacgccgg gcgtggcgca agcggccaga cccagcgaca
      541 acaaggcagc agccagtagt ttcatgattc gacctccagt gaattgcgcc agcgccacaa
      601 caggaagtcg gccagcgcct tgaacagcag cggcagcagg gcataagcga agaccagggc
      661 ggtttgtccc tgatcgctgc ccggccggta atcgaagacg ccgagtagcg gcagggcgat
      721 gccggcggcc agcgccaggt tgagcttggc gaagaagttc cacaccccga aacaggcacc
      781 cgaacgcccc tggcgctcgc cgaggtcggc cgccagcgcc gccggcaggg ccaggtcggc
      841 ccccagcgcc agccccgacg ccacacagac gacggcgaac agcgcgacat cgcccggccc
      901 gagcaggccg gcgccggcaa aggcgagcat ggccacgccc atcgccagca accaggcgcg
      961 cggccggccg atgcgcacgg acagcttcag ccacagtggc agcgacgccg ccccggccag
     1021 gaagtaggca gccagcagcg gcccgctggc gctctccgcc tgcaggatgt cggcgacgaa
     1081 gaacaggaac agcgtcgccg gaatcgccgc agcgaagctg ttgcagatga agaccagcag
     1141 cagccggcgg aaggcggtat cgcgcagcac cgcgcgcagc gccggcagca gggcgctgct
     1201 gaccgccacc cgagggtcac cttcgccaac ccgggccagg gtgacgccgc cggcgagcac
     1261 cagcagcacc ggcaggaccc aggccagacg ggcgatgccg tcgttgagtg cggccgccag
     1321 cagggccggc agggcggcag cgacgagcac gccgagcaga ccgaaagcct cgcgcgaggc
     1381 aaccagccgg gtgcgctccg gcgcgtcgct gccgaccgcc gcgccccagg cctggtaggc
     1441 gacggtggcg gcagagaaac cagcgtaggt cagcagcagg ctggcgagca gccagggcgc
     1501 ggcgtgttcc actggcgggt tgagcagggc aacgaaaccg acggcgaagg gcaacaggga
     1561 gacggccacc atgcgccggc gcgacacccg gtcggccagc cagcccagcc agggatcgat
     1621 gccggcgtcg agcaagcggg cgagcagcag gatggcaccg agtagaccga ggtcgagacc
     1681 ggtgacattg gcgtagaagg ccggcacatg gacgtacaac g
//
LOCUS       DAOSDX010000690         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23508_length_1719_cov_2.683293, whole genome shotgun sequence.
ACCESSION   DAOSDX010000690 DAOSDX010000000
VERSION     DAOSDX010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23508_length_1719_cov_2.683293"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLN02_13175"
     CDS             <1..223
                     /locus_tag="PLN02_13175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOI53327.1"
                     /translation="KFDSSKDRNDPFQFPLGQGHVIKGWDEGVQGMKIGGTRKLTIPA
                     HLGYGARGAGGVIPPNATLVFEVELLDLQ"
     gene            220..1572
                     /locus_tag="PLN02_13180"
     CDS             220..1572
                     /locus_tag="PLN02_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOI53328.1"
                     /translation="MTQPDDPWAKWRKPTDDAVDRKPSAAVAPIDTPKPVKPQPAAAP
                     ETPVAASAPAKPAAPVEARDDVAPTPPTGTLGVLKKPGSETTGRKRPVRGGALARKPR
                     ETPAAPPRQREGARPEGGRPIAAKPRQERPGGEWQEKRRPDGGRQEKFGQGRDGQNRD
                     RHPTGENPWARKPASEAPRGNAGARPPRPERAERPASPPPAVVASGEAPSGVRLSKVM
                     SERGLCSRREADLWIERGWVFVDGVQVSELGSRIDPDARIEISTEAKKDQAKAVTILL
                     HKPVGYVSGQPEPGCIPAVTLITAENQVPQSGGPEFKPWMLRGLAPAGRLDVDSTGLL
                     VLTSDGRVAKRLIGEDSEAEKEYLVRVSGQMIKGGLDLLRHGLELDGKPLKPAWVKQL
                     NEDQLHFILKEGKKRQIRRMCELVGLKVIGLKRVRIGRIRLGDLPMGQWRFLRDDEAF
                     "
BASE COUNT          306 a          573 c          610 g          230 t
ORIGIN      
        1 caagttcgat tcgagtaagg atcgcaacga ccccttccag tttccgctcg gccagggcca
       61 tgtcatcaag ggctgggacg aaggcgtcca gggcatgaag atcggcggca cccgcaagct
      121 gactattccg gcgcatctcg gttacggcgc ccgcggcgct ggcggcgtca tcccgccgaa
      181 cgccacgttg gtcttcgagg tcgagctcct cgatctgcaa tgacccagcc ggacgatccc
      241 tgggccaagt ggcgcaagcc gacagacgat gcggtcgacc gcaagccgtc cgccgcagtt
      301 gcgccgatag acacgcctaa gccagtcaaa ccgcagccgg ccgccgcgcc ggagacgccg
      361 gtggcggcaa gtgccccggc aaaacccgcg gcgccggtcg aggcgcggga tgacgtagcg
      421 ccaacgccgc cgacggggac gctcggtgtg ctcaagaagc caggaagcga aaccaccggc
      481 cgcaagcgcc cggtgcgcgg cggtgccctg gctcgcaagc cgcgtgagac cccggctgcg
      541 ccgccgcggc aacgcgaagg cgcgcgtccg gaaggtgggc gtccgatcgc cgccaagccg
      601 cgccaggaac gcccgggcgg ggagtggcag gagaagcgcc ggccggatgg cgggcgccag
      661 gaaaaattcg ggcaaggcag ggacgggcag aaccgggatc gtcacccgac cggcgaaaat
      721 ccctgggccc gcaagccagc cagcgaggcg ccgcgcggca acgccggggc gcgtccgccg
      781 cggccagagc gtgccgagcg gcccgccagc ccgccgccag cggtcgtcgc cagcggcgag
      841 gcgccgagcg gcgtccgcct gtccaaggtg atgtccgagc gcggcctttg ctcgcggcgc
      901 gaagccgacc tctggatcga gcgcggctgg gtttttgtcg atggcgtgca ggtcagcgaa
      961 ctgggttcgc ggatcgaccc cgacgcgcgc atcgaaatat cgacagaagc caagaaggat
     1021 caggccaagg cggtgacgat cctgctgcac aagccggtcg gctacgtttc cgggcagccg
     1081 gaaccgggct gcattccggc ggtgaccctg attaccgcgg aaaaccaggt gccgcagtcg
     1141 ggcgggcccg aattcaagcc gtggatgctg cgcggcctgg cgccggccgg ccgcctcgac
     1201 gtcgattcga ccggtctcct ggtgctgacc agcgacggtc gcgtcgccaa acgcctgatc
     1261 ggcgaggaca gcgaagccga aaaggaatac ctggtgcgtg tttccgggca gatgatcaag
     1321 ggcggcctcg acttgctccg gcacggtctg gaactcgacg gcaagccttt gaagccggcc
     1381 tgggtcaagc agttgaacga ggaccagctg cacttcatcc tcaaggaagg gaagaagcgg
     1441 cagatccgcc gcatgtgcga gctggtcggg ctgaaggtga tcggtctcaa gcgcgtgcgc
     1501 atcggtcgca tccgcctggg cgatctgccc atggggcagt ggcgcttcct gcgcgacgac
     1561 gaggcattct gacgtcgccg tgacgcagcg cccataaaaa acgccggccg ggtcgccccg
     1621 ccggcgtttt tcattggtgt aagggtctca ggccgtaccg gtcggagcgg cggcgcggac
     1681 cggcggcatc atcacgcatt cgccctcgat caccgtctt
//
LOCUS       DAOSDX010000691         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23519_length_1718_cov_2.665063, whole genome shotgun sequence.
ACCESSION   DAOSDX010000691 DAOSDX010000000
VERSION     DAOSDX010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23519_length_1718_cov_2.665063"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1274
                     /locus_tag="PLN02_13185"
     CDS             108..1274
                     /locus_tag="PLN02_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI53329.1"
                     /translation="MPIRLTALAAALLLAGAAHAADQIKVGFVSTLSGPGAGLGVDIR
                     DGFQLAMKQLNGKLGGLPAEVLVADDQQNPDVAKQATDKLLKKDKVDFMTGIVFSNLM
                     LAVGPAVFQNQTFYISANAGPSQYAGEQCNPFFFNVAWQNDNLHEAVGKYVQDKGFKN
                     VVIVTPNYPGGKDAVSGFKRYYKGQVGEEIYTKLGQLDYAAELAKVRAGKPDALFFFL
                     PGGMGINFVKQFVAAGLSRDTQLFAPGFSADEDVIKAVGAPMMGMFNSSHWAHDMDNA
                     ENKRFVAAFEKEYGRLPSLYASQGYDAALMMDAAVRDVKGNVADKKALQKALEAKRFK
                     SVRGDFKFNSNHYPVQNYYLRVIGKDAQGRVTNKTMGSIFTNHADAYAAQCKMK"
     gene            1271..>1718
                     /locus_tag="PLN02_13190"
     CDS             1271..>1718
                     /locus_tag="PLN02_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI53330.1"
                     /translation="MTQLVLEQALNGLQFGLMLFLLAAGLTLVFGIMDCINLAHGSLY
                     MVGAYFVAAAAQAADSFWLGLGAGVAATAVLGLLLELTLFRQLYRRDHLSQVLATFAL
                     ILIANEAVRLIWGPAPLMLNPPEALAGPVEIGGFFYPAYRLLIIGCG"
BASE COUNT          293 a          584 c          531 g          310 t
ORIGIN      
        1 ttcgttcatg gggcgcgaat atacccgaat ttgccggtcc accgggacgg tatctgcttg
       61 ttcctgcgct acaatttgcc gctcgcccca ctgctccgga gaaccagatg ccgatccgcc
      121 tgaccgccct cgccgccgct ttgctgctgg ccggcgccgc ccacgccgcc gaccagatca
      181 aggtcggctt cgtttccacc ctgtccggtc ccggggccgg tctcggcgtc gacatccgcg
      241 acggtttcca gctggcgatg aaacaactga acggcaagct cggcggcctg ccggccgaag
      301 tcctggtcgc cgacgaccag cagaacccgg atgtcgccaa gcaggcgacc gacaagctgc
      361 tgaagaagga caaggtcgat ttcatgaccg gcatcgtctt ctccaacctg atgctggccg
      421 tcgggccggc cgttttccag aaccagacct tctacatctc ggccaatgcc ggcccgtcgc
      481 agtacgcggg cgagcagtgc aaccccttct tcttcaacgt cgcctggcag aacgacaacc
      541 tgcacgaggc ggtcggcaag tacgtgcagg acaagggctt caagaatgtc gtcatcgtca
      601 cccccaacta cccgggcggc aaggacgcgg tgtccggctt caagcgctac tacaagggcc
      661 aggtcggcga ggagatctac accaagctcg gccagctcga ttacgccgcc gaactggcca
      721 aggtccgcgc cggcaagccg gatgcgctgt tcttcttcct gccgggcggc atgggcatca
      781 acttcgtcaa gcagttcgtc gccgccggcc tgtcgcgcga cacccagctc ttcgcgccgg
      841 gcttctcggc cgacgaggac gtgatcaagg cggtcggcgc gccgatgatg ggcatgttca
      901 actcgtcgca ctgggcgcac gacatggaca atgccgagaa caagcgcttc gttgccgcct
      961 tcgagaagga atacggtcgc ctgccgtcgc tctacgcctc gcagggttac gacgcggcgc
     1021 tgatgatgga cgccgcggtg cgcgacgtga agggcaacgt cgccgacaag aaggcgctgc
     1081 agaaggcgct ggaggccaag cgcttcaagt cggtgcgcgg cgacttcaag ttcaacagca
     1141 accattaccc ggtgcagaac tactacctgc gcgtgatcgg caaggacgcc cagggtcgcg
     1201 tcaccaacaa gacgatgggc agcatcttca ccaatcatgc tgatgcctac gcggctcagt
     1261 gcaagatgaa gtgacccagc tggttctcga gcaggccctc aacggcctgc agttcggcct
     1321 catgctgttc ctgctggccg ccgggctgac gctcgtcttc ggcatcatgg actgcatcaa
     1381 cctggcgcat ggctcgctgt acatggttgg cgcctacttc gtcgccgccg cggcgcaggc
     1441 cgccgattcg ttctggctcg gcctgggcgc cggggtggcc gctaccgccg tgcttggcct
     1501 gctgctcgaa ctgacgctgt tccgccagct ttaccggcgc gaccacctgt cgcaggtgct
     1561 ggcgaccttt gccctgatcc tgatcgccaa cgaggcggtg cgcctgatct gggggccggc
     1621 gccgctcatg ctcaacccgc cggaagcgct ggccgggccg gtcgagatcg gcggtttctt
     1681 ctatccggcc taccggctgc tgatcatcgg ctgcggcc
//
LOCUS       DAOSDX010000692         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23540_length_1717_cov_2.741877, whole genome shotgun sequence.
ACCESSION   DAOSDX010000692 DAOSDX010000000
VERSION     DAOSDX010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23540_length_1717_cov_2.741877"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLN02_13195"
     CDS             complement(<1..762)
                     /locus_tag="PLN02_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOI53331.1"
                     /translation="MKTPVLAARTADLAEIDQFCDALWLEDGLAKATLASYRSDLGRL
                     AGWLAGHGHEALLDLRESTLAGFVAELSRSTRASSQARYLSTLRRFYRWQVSRGRIVV
                     DPTLQLANPSLPARLPKVMSEAQVGALLAAPDLETPLGLRDRAMLETLYATGLRVSEL
                     VGLQLHSISLNDGVLRAFGKGSKERLVPLGEQAIDWIARYLRESRADILGARQSDALF
                     VTARGGAMTRHAFWHLIKRYALQAGIDPAKLSPHVL"
     gene            complement(737..1213)
                     /locus_tag="PLN02_13200"
     CDS             complement(737..1213)
                     /locus_tag="PLN02_13200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOI53332.1"
                     /translation="MSDHQAIVAAPGFALGIRCSDDEITAIDFLEPRAAQAPATPLAA
                     EAVRQLEAYLADPNFVFGLPLRPAGTAFQRRVWAQIAAIPGGRTQTYGELARALHNAP
                     RAVGQACGANPYPLVVPCHRVVASHGGLGGFARERGGFLLSIKRWLLQHENAGFGR"
     gene            1345..>1717
                     /locus_tag="PLN02_13205"
     CDS             1345..>1717
                     /locus_tag="PLN02_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOI53333.1"
                     /translation="MHPRLSSLFAALATTTLIAACGQQEAPSAQSSAPAPAAKIVVGL
                     DDNFPPMGFRDAKNELVGFDIDLAREAAKRLGVEVEFKPIDWNAKEAELSGKRVDALW
                     NGLTITEERRKNIGFTTAYMEN"
BASE COUNT          279 a          558 c          619 g          261 t
ORIGIN      
        1 cagcacgtgc ggcgacagct tcgccgggtc gatgccggcc tgcagcgcgt agcgcttgat
       61 caggtgccag aaggcgtgac gggtcatggc gccgcctcgc gcggtgacga acaaggcgtc
      121 gctctgccgg gcgccgagga tgtcggcgcg gctttcgcgc aggtagcggg caatccagtc
      181 gatcgcctgc tcgccgagcg gcaccaggcg ttccttgctg cccttgccga aggcgcgcag
      241 cacgccgtcg ttgaggctga tcgaatgcag ttgcaggcca accagttcgg aaacgcgcag
      301 gccggtcgcg tacagcgtct ccagcatggc gcggtcgcgc aagccgagcg gcgtctccag
      361 gtcaggggcg gcgagcaggg cgccgacctg ggcttccgac atcaccttgg gcaggcgggc
      421 gggcaggctg gggttcgcta gctgcagcgt tggatcgacg acgatgcggc cgcggctgac
      481 ctgccagcga tagaagcgac gcaaggtgga aagataacgc gcctgcgaac tggcgcgcgt
      541 gctgcgcgac aattcggcga cgaagccggc cagcgtgctc tcgcgcaggt cgagcagggc
      601 ctcgtggccg tgtccggcga gccagccagc gagccggccg aggtcgctgc ggtagctggc
      661 gagcgtcgcc ttggccaggc cgtcttccag ccacagcgcg tcgcagaact ggtcgatttc
      721 ggcgaggtcg gccgttctag cggccaaaac cggcgttttc atgctgcaac agccagcgtt
      781 tgatcgagag caggaaaccg ccgcgctcgc gggcgaagcc gccgagtccg ccatggctgg
      841 cgacgacccg gtgacagggc acgaccagcg ggtagggatt ggcgccgcag gcctggccga
      901 cggcgcgcgg cgcgttgtgc agcgctcgcg ccagctcgcc gtaggtctgc gtgcggccgc
      961 cgggaattgc cgcgatctgc gcccacaccc ggcgctggaa cgcggtgccg gccgggcgta
     1021 gcggcaggcc gaaaacaaaa ttcgggtcgg ccaggtaggc ttccagttgg cgcaccgcct
     1081 cggcggcgag cggcgttgcc ggcgcttgcg ccgcccgtgg ttcgaggaag tcgatggcgg
     1141 tgatctcgtc gtcgctgcag cggatgccga gcgcgaagcc gggggcggcg acgatggctt
     1201 gatggtcgga catgcgggtt cctggtcggc gggtcggcga agtgtataac gtgccgtgcg
     1261 cggtgtgctc attgcgaagc gggcggcggt tttgggggat aattgccggc tattcgaaaa
     1321 atcacctttt ccaggagttc acgcatgcat ccgcgccttt cctccctgtt cgccgcgctc
     1381 gccaccacca ccctgatcgc cgcctgcggc cagcaggaag cgccgtcggc gcagagctcc
     1441 gcgccggcgc cggccgccaa gatcgtcgtc ggcctcgacg acaacttccc gccgatgggt
     1501 ttccgcgacg ccaagaacga actggtcggt ttcgacatcg acctcgcccg tgaagctgcc
     1561 aagcgcctcg gcgtcgaagt cgagttcaag ccgatcgact ggaacgccaa ggaagccgaa
     1621 ctgagcggca agcgcgtcga tgcgctgtgg aacggcctca ccatcaccga agagcgccgg
     1681 aagaacatcg gcttcaccac cgcctacatg gagaacc
//
LOCUS       DAOSDX010000693         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23544_length_1717_cov_2.190734, whole genome shotgun sequence.
ACCESSION   DAOSDX010000693 DAOSDX010000000
VERSION     DAOSDX010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23544_length_1717_cov_2.190734"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLN02_13210"
     CDS             complement(<1..352)
                     /locus_tag="PLN02_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI53334.1"
                     /translation="MVHNFDVLIIGSGLAGQSAALRLAGHCRVALISKRKLEDSASGW
                     AQGGIAAVLDNQDSIEAHIRDTFVAGAWLNDEKATRFVVENGRRAIDWLIEQGVPFTK
                     DESGYHLTREGGHSA"
     gene            502..1101
                     /gene="rpoE"
                     /locus_tag="PLN02_13215"
     CDS             502..1101
                     /gene="rpoE"
                     /locus_tag="PLN02_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HOI53335.1"
                     /translation="MSEREVDQLLVERAQGGDQTAFDQLVSKYQRKLGRLLSRFIRDP
                     AEVEDVAQEAFIKAYRALPSFRGDSAFYTWLYRIGINTAKNYLVSQGRRAPTSTEFDA
                     DEAEGFEDAALLRDINTPESLLMSKQIGETVNAAMDALPEELRTAIVLREIEGLSYEE
                     IASQMDCPIGTVRSRIFRAREAVASKLRPLLDIAPDRRW"
     gene            1105..1557
                     /locus_tag="PLN02_13220"
     CDS             1105..1557
                     /locus_tag="PLN02_13220"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOI53336.1"
                     /translation="MSAEENIAHAVVRSLEADMVEVEIEQGCGRCHEEGGCGGQNLTQ
                     MFAAGPRHYRLRNTVGAGIGDRVAIAIAPGSVRRSANFAYGVPLAALFVGALAGGQLA
                     GDNGAMLGAAAGLLLAFLYLAWRRPAASQVAAPQIVSRLPTKSGSCSR"
BASE COUNT          315 a          520 c          564 g          318 t
ORIGIN      
        1 gggcgctatg tccgccctca cgggtcaggt ggtagccaga ctcgtccttg gtgaaaggaa
       61 ccccttgttc gatcagccag tcgatggcgc ggcgaccatt ttcgacgacg aagcgggtcg
      121 ccttctcgtc gttgagccag gcaccggcga cgaaggtgtc gcggatgtgc gcctcgatcg
      181 aatcctggtt gtcgagcacg gcggcaatgc caccttgcgc ccagccggag gcggagtcct
      241 cgagcttgcg cttgctgatc agcgcgaccc ggcaatgtcc ggcgagccgc aatgcggcgg
      301 attggccggc caggccactg ccgatgatga gcacatcaaa gttatgcacg atgacttact
      361 gggaaaaatg gtccggggcg caatatagca cgcaggaaac gcttcatgca gttacagaaa
      421 atcacacgcg ggcggggatg gcgtttacgg ggctgcgtta tactgcgctc cgacaagaat
      481 acttcccggg gaaaaacggc catgagcgag cgcgaggtcg atcagttact ggtcgagcgg
      541 gcgcaaggcg gcgatcagac tgccttcgac caattggtga gcaagtacca gcgcaagctg
      601 gggcgcttgc tgtcgcgctt catccgcgat ccggccgaag tcgaggacgt ggcgcaggaa
      661 gccttcatca aggcctaccg cgccttgcca tcgttccgcg gtgacagcgc cttctacacc
      721 tggctgtacc ggatcggcat caacaccgcc aagaactacc tggtttccca ggggcggcgg
      781 gcgccgacca gcaccgaatt cgacgccgac gaagccgaag gcttcgagga cgccgcgctg
      841 ctgcgcgaca tcaacacccc ggaaagcctg ctgatgtcga aacagattgg cgaaacggtg
      901 aacgcggcga tggatgccct gcccgaggag ttgcgcacgg cgatcgtcct gcgcgaaatc
      961 gaggggcttt cctacgagga aattgccagt cagatggatt gtccgatcgg cacggtacgc
     1021 tcgcgcatct ttcgcgcccg cgaagcggtg gccagcaagc tgcgtccctt gctggacatt
     1081 gcgccagacc ggcgctggtg aaacatgtct gctgaagaaa acattgccca tgcggttgtc
     1141 cgttcgctcg aagcggacat ggtcgaggtc gaaattgaac aaggttgtgg tcgttgccac
     1201 gaggaaggcg gctgcggcgg ccagaacctg acccagatgt ttgccgccgg gccgcgccac
     1261 tatcgtctgc ggaacaccgt cggtgccggc attggcgacc gcgtggcgat tgccatcgcg
     1321 ccgggcagtg tgcgccgtag cgccaacttt gcctatggtg tgccgcttgc tgccttgttc
     1381 gtcggtgcgc tcgctggcgg gcaactggct ggcgacaacg gcgccatgct gggcgctgct
     1441 gccggactgt tgctggcttt tctctacctt gcctggcgca ggccagcggc atcgcaagtg
     1501 gcagcgccgc agatcgtttc ccgcttgccg accaaatcgg ggagttgcag tcgatgaagc
     1561 gttttctggc cctgctcgcg tgcagcctga cgatcggatt cgtccaggcg caaaaccggt
     1621 cgctgcccga ttttgccgac cttgccgaaa agcagggggc tgcggtggtc aatatcagca
     1681 ccctgcaggc gccgcgtggg cgttccggcg cttcgcc
//
LOCUS       DAOSDX010000694         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23626_length_1712_cov_2.402535, whole genome shotgun sequence.
ACCESSION   DAOSDX010000694 DAOSDX010000000
VERSION     DAOSDX010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23626_length_1712_cov_2.402535"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLN02_13225"
     CDS             <1..180
                     /locus_tag="PLN02_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53337.1"
                     /translation="GPGQRLTIAADGAPLLVSGFDAGRTTALRVEFRAPAANWRLRLA
                     RRLLAPRGTLSGAWQ"
     gene            177..572
                     /locus_tag="PLN02_13230"
     CDS             177..572
                     /locus_tag="PLN02_13230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOI53338.1"
                     /translation="MNARQPCDDFRLLSSNPLVRLDGGASDLSFKVGKRHLNGAGTLH
                     GGVLASVFDSALGECVYRQAQHSGRPAALVVTTQLDIHYCAPAHVGERVAVAVEVLRL
                     GGRLAVATARASVAERLVGTATGSFYLTR"
     gene            611..1108
                     /locus_tag="PLN02_13235"
     CDS             611..1108
                     /locus_tag="PLN02_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOI53339.1"
                     /translation="MSTPPFVHLDDLELHHEAHGERFASANAGVGARLGLKGLGCRLS
                     EVPPGKAAWPRHAHLANDELVLILAGSGRLRHGDAEYAVAAGDLLGFPGGDPAAAHQI
                     VNDGSVPLRYLCVSTQQAPDIAIYPDSGKFFVVAGSAPGGDKAARTFAHIGRLADAVD
                     YWADE"
     gene            complement(1120..>1712)
                     /locus_tag="PLN02_13240"
     CDS             complement(1120..>1712)
                     /locus_tag="PLN02_13240"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOI53340.1"
                     /translation="FDRLAANSDRQNQLAGLSSPYGQRIAKLSEGLSSYDGLTLNIKA
                     YISSEVNAFAMANGVIRVYSGLMDRFSDDEIRYVIGHEIGHVQAGHTKARIQTALRTS
                     ALRNAVASAGGTAAGVADSALGSLFEQVVHAKHSQANENEADDRALAFMREKQYPLQA
                     CVSALEKLEGLGGNNASWLSTHPSPRERADRIRTQI"
BASE COUNT          240 a          587 c          608 g          277 t
ORIGIN      
        1 ggccctggcc agcgcctgac catcgccgcc gacggcgcgc cgctgctggt cagcggtttc
       61 gacgccgggc ggacaaccgc actgcgcgtc gaattccgcg ccccggcggc caactggcgg
      121 ctgcgcctgg cacggcggct gctggcccca cgcggcacgc tgagcggagc ctggcaatga
      181 acgcccgcca gccctgcgac gacttccgcc tgctgtcgag caacccgctg gtgcgtctgg
      241 acggcggcgc cagcgacctc tcgttcaagg tcggcaagcg ccatctcaac ggcgccggca
      301 cgctgcacgg cggcgtcctc gccagcgtct tcgacagcgc gctgggcgag tgcgtctatc
      361 gccaggcgca gcacagcggc cgacccgccg ccttggtcgt gaccacgcaa ctcgacatcc
      421 actactgcgc accggcccat gtcggcgaac gggtggcggt cgccgtcgag gtgctgcgcc
      481 tgggcggccg gctggcggtg gcgacggcgc gcgcctcggt ggcggaacgg ctggtcggca
      541 cggcgaccgg ctccttctac ctgacgcgct gaatgtggtt tatcgtcgtc cgctcaagga
      601 ggaaaacccc atgtctacac cccctttcgt ccatctcgac gatctcgaac tgcaccacga
      661 agcgcacggc gagcgcttcg cctcggccaa tgccggcgtc ggcgcccggc tcggcctgaa
      721 gggcctgggc tgtcggctga gcgaggtgcc gccgggcaag gccgcctggc cgcgccacgc
      781 ccatctggcc aacgacgagc tggtactgat cctcgccggc agcggccgcc tgcgccacgg
      841 tgatgcggaa tacgcggttg ccgccggcga cctgctcggc tttcccggcg gcgatccggc
      901 ggcggcgcac cagatcgtca acgacggcag cgtgccgcta cgctacctct gcgtgtcgac
      961 gcaacaggcg ccggatatcg cgatctatcc cgattccggc aagttcttcg tcgtcgccgg
     1021 cagcgcgccg ggcggcgaca aggcggcgcg gactttcgcc cacatcggcc gcttggccga
     1081 cgcggtcgat tactgggcgg acgagtgagg tcgggaaact cagatctggg tgcggatgcg
     1141 gtcggcgcgc tcgcgcgggc tggggtgggt ggacagccag gaggcgttgt tgccgcccag
     1201 gccttcgagt ttttccagcg cgctgacgca ggcctgcagc ggatactgct tttcgcgcat
     1261 gaaggcgagg gcgcggtcgt cggcttcgtt ctcgttggcc tgcgagtgct tggcgtggac
     1321 cacctgttcg aacaggctac ccagcgcgga atcggcgacg ccggcggcgg taccgccagc
     1381 cgaggcgacg gcgttgcgca gcgcgctggt gcgcagcgcg gtctggatgc gcgccttggt
     1441 gtggccagcc tggacgtgac cgatctcgtg gccgatcacg tagcggattt cgtcgtcgct
     1501 gaaacggtcc atcagcccgc tatagacgcg gatcacgccg ttggccatgg cgaaggcgtt
     1561 cacttccgac gagatatagg ccttgatgtt gagcgtcagg ccgtcgtagc tgctcagccc
     1621 ctccgacaac ttggcaatgc gctggccgta gggcgaggac aagccggcga gctggttctg
     1681 gcggtcggaa ttggcggcca ggcggtcgaa gt
//
LOCUS       DAOSDX010000695         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23752_length_1704_cov_2.600364, whole genome shotgun sequence.
ACCESSION   DAOSDX010000695 DAOSDX010000000
VERSION     DAOSDX010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23752_length_1704_cov_2.600364"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLN02_13245"
     CDS             <1..229
                     /locus_tag="PLN02_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF615 domain-containing protein"
                     /protein_id="HOI53341.1"
                     /translation="LERFRTRLLEEEATLTEIANQWPGADLQQLRQLRRNALKEQEAG
                     KPPKSYRAIFQFLKDLDGTPTGSAETEHDDA"
     gene            216..806
                     /gene="mog"
                     /locus_tag="PLN02_13250"
     CDS             216..806
                     /gene="mog"
                     /locus_tag="PLN02_13250"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HOI53342.1"
                     /translation="MTTPDTLVVGLVSISDRASTGIYEDKGIPALQEWLGSALTTPWR
                     AETRLIADDRPGIEATLIELVDDCGCHLVLTTGGTGPAPRDVTPEATLAVADKEMPGF
                     GEEMRRISLNFVPTAILSRQVAVIRKQALIVNLPGQPKSIRETLEGVRAADGSIRHVG
                     IFAAIPYCIDLIGGPYAETDPQVIQAFRPKSALRPR"
     gene            complement(810..>1704)
                     /locus_tag="PLN02_13255"
     CDS             complement(810..>1704)
                     /locus_tag="PLN02_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53343.1"
                     /translation="LEIELAERFIAHYLMAFDFPQHGEHDCVYWVDLALAQPPLRLAR
                     MPAEATPTLRFFRPGRAHHEMAELLQLLERGGDVPADIMLGGQYPAKTLIPVLRHLAA
                     YLAPIPPQRRHDRHRVKHRMSVMSGLVNAYVVFSGEFGGRPAGLQIESWVVENVSRGG
                     FGAYVNSIPGEWLKVGALLALQPEGGENWVLGCVRRYHRESEDEARVGIETLARAVVA
                     VELKPKMISSYAAVGGIPALLLQENNAPGELRVVLPPYTFDPREALELVLDDRRNLLE
                     PLLVIEQTNDYELARYRVLPG"
BASE COUNT          324 a          602 c          529 g          249 t
ORIGIN      
        1 cctggaacgt ttccgcacgc gcctgctgga agaggaagcg acgctgaccg agatcgccaa
       61 ccagtggccc ggcgccgacc tccagcaatt gcgccagttg cgccgcaacg cgctgaaaga
      121 acaggaagcc ggcaagccgc cgaagagcta ccgcgccatc ttccagttcc tcaaggacct
      181 cgacggcacc ccgaccggca gcgcggagac cgagcatgac gacgcctgac accctggtgg
      241 tcggcctggt ctcgatcagc gaccgtgcct cgaccggcat ctacgaagac aagggcatcc
      301 cggcgctgca ggaatggctg ggcagtgcac tgaccacgcc gtggcgcgcc gaaacccggc
      361 tgatcgccga cgaccggccg ggaatcgaag caacgctgat cgagctggtc gatgattgcg
      421 gctgccacct ggtactgacc accggcggca ccggcccggc gccgcgcgac gtcacgccgg
      481 aagcgacgct ggccgtcgcc gacaaggaaa tgcccggctt cggcgaggaa atgcggcgca
      541 tcagcctgaa cttcgtgccg accgcgatcc tgtcgcggca ggtggcggtg atccgcaagc
      601 aggcgctgat cgtcaatctg cccgggcaac cgaaatcgat ccgcgaaacg ctcgaaggcg
      661 tccgcgccgc cgacggcagc atccgccacg tcggcatctt cgccgccatt ccctactgca
      721 tcgacctgat cggcggcccc tacgccgaaa ccgatccgca ggtgatccag gctttccggc
      781 cgaaatcggc gctccgcccg cgctgaccct cagccgggca ggacgcggta gcgtgccagc
      841 tcgtaatcgt tggtctgctc gatgacgagc agcggttcga gcaggttgcg ccggtcatcg
      901 agcaccagct caagagcttc gcgcggatca aaggtatagg gcggcaacac gacgcgcaat
      961 tcgcccggcg cattgttctc ctgcaacagc agcgccggaa tcccaccaac cgcggcgtag
     1021 ctggaaatca tcttcggttt cagttcgacg gcgaccaccg cccgcgccag cgtctcgatg
     1081 ccgacccgcg cctcgtcctc gctctcccgg tgatagcggc ggacgcaacc gagcacccag
     1141 ttctcgccgc cttccggctg cagggcgagc aaggcgccga ccttcagcca ctcgccggga
     1201 atgctgttga cgtaggcgcc gaaaccgcca cggctgacgt tctcgaccac ccagctctcg
     1261 atctgcaggc cggccgggcg accgccgaac tcgccggaga agacgacata ggcattgacc
     1321 aggccgctca tcaccgacat ccgatgcttg acccggtgcc ggtcgtgccg acgctgcggc
     1381 ggaatcggcg ccagataggc cgccagatgc cgcaacaccg gaatcagcgt cttcgccgga
     1441 tactggccac cgagcatgat gtcggctggc acatcgccgc cgcgctcgag tagctgcagc
     1501 aattccgcca tttcgtgatg cgcccttccc gggcggaaaa agcgcagggt cggcgtcgct
     1561 tccgccggca tccgcgccag acgcagcggc ggctgggcga gcgccagatc gacccaatag
     1621 acgcaatcat gttcgccgtg ctgtggaaaa tcgaaagcca tcaggtaatg cgcgatgaat
     1681 cgctcggcca gttcgatttc cagc
//
LOCUS       DAOSDX010000696         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23807_length_1701_cov_2.609964, whole genome shotgun sequence.
ACCESSION   DAOSDX010000696 DAOSDX010000000
VERSION     DAOSDX010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23807_length_1701_cov_2.609964"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="PLN02_13260"
     CDS             complement(<1..125)
                     /locus_tag="PLN02_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOI53344.1"
                     /translation="MNKARIGIVGAGGRMGRMLIEAVLKDAGAVLGAAFDVPGSPA"
     gene            complement(130..582)
                     /gene="bamE"
                     /locus_tag="PLN02_13265"
     CDS             complement(130..582)
                     /gene="bamE"
                     /locus_tag="PLN02_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HOI53345.1"
                     /translation="MACTLPAACSYKPSFINEYKIDVQQGNVLTQDMVAQLKPGQTRE
                     QVRFILGSPLITDIFHQQRWDYVYRFREGSTGAVQARKFSVFFDANGRLERVSGDVEV
                     ATVAELTAPVERMRVVDLGTISPEAVAEEAPPPEEPGFFRRMIKKIGF"
     gene            708..1160
                     /gene="fur"
                     /locus_tag="PLN02_13270"
     CDS             708..1160
                     /gene="fur"
                     /locus_tag="PLN02_13270"
                     /inference="COORDINATES: protein motif:HMM:NF006999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HOI53346.1"
                     /translation="MSDPQSLKSIGLKATFPRLKILELFEKGTMRHMTAEDVYRMLIA
                     ENMDIGLATVYRVLTQFEQAGLLERHFFEAGKAVFEINHGKHHDHLVCVECGRVEEFY
                     DPEIERRQKLIAEERGFKIQDHALYIYGVCSHHQANPGQQADESDASE"
     gene            complement(1166..>1701)
                     /locus_tag="PLN02_13275"
     CDS             complement(1166..>1701)
                     /locus_tag="PLN02_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOI53347.1"
                     /translation="IMRRLALSSGRFEIALPALDNPAVYGLEQVEFRIGGLAGNDARP
                     LAKVASGGELSRISLAIQVLTSRSASVPTLIFDEVDVGIGGGVAEIVGRLLRELGGER
                     QVLCVTHLAQVAAQADWQWQVSKTTRDGVTRSAIQPLDDAGRVGEIARMLGGVEVTGI
                     TLEHAREMLRAGAEQAR"
BASE COUNT          350 a          557 c          503 g          291 t
ORIGIN      
        1 gccggacttc ccggaacatc gaaagcggcg ccgagcacag cccccgcatc cttgagcacc
       61 gcctcgatca acatgcggcc catgcgaccg ccggcaccaa cgatgccgat tcttgccttg
      121 ttcatggttc tagaatccga tcttcttgat catgcgccgg aaaaaccccg gctcttcggg
      181 cggcggcgcc tcttcggcaa cggcttccgg ggaaatcgtc cccaaatcga ccacccgcat
      241 ccgttccacc ggcgccgtca attcggccac cgtcgccacc tcgacatcgc cgctcacccg
      301 ctccaggcgg ccgttggcgt caaagaagac ggaaaattta cgagcctgca cagcccccgt
      361 gctgccttca cgaaagcggt atacataatc ccagcgctgc tgatgaaaga tatcggtgat
      421 cagcgggctg ccgaggatga aacgaacctg ttcgcgggtc tgcccgggtt tgagttgggc
      481 gaccatgtcc tgggtcagca cgttgccctg ctggacgtcg atcttgtatt cgttgatgaa
      541 actcggcttg taggaacacg ccgcaggcag cgtgcaggcc aaggcaagaa gcaggggacg
      601 gtaacagcgc atgcgggctt ccggaaattt cgtaacggcg ttgaagcggt atatcatacc
      661 cgaaatcctc cttgcccagc cccgcgtaac atcaggaacc caacacgatg agtgacccgc
      721 agagcctcaa aagcatcggc ctgaaagcca ctttcccgcg cctcaagatt cttgagttgt
      781 tcgaaaaggg tacgatgcgg cacatgacgg cggaagatgt ctatcgcatg ctcatcgcgg
      841 aaaacatgga catcggtctg gcgacggttt accgggtgct gacccagttc gaacaggccg
      901 gcctgcttga gcgccatttt ttcgaagccg gcaaggcggt cttcgaaatc aaccacggca
      961 agcaccacga ccacctggtc tgtgtcgaat gcggccgggt cgaagagttc tacgatcctg
     1021 aaatcgaacg ccggcagaaa ctgattgccg aagagcgcgg cttcaagatc caggaccacg
     1081 cactctacat ctacggcgtc tgcagccacc accaggccaa tcccgggcag caagcggacg
     1141 aatccgacgc cagcgaatag ctgcgtcagc gcgcctgctc ggcgccggca cgcagcattt
     1201 cgcgggcgtg ctccagcgtg attccggtca cttcgacgcc gcccagcatg cgagcgattt
     1261 cgccgacgcg gccggcgtcg tcgagcggct gaatcgccga gcgcgtgacg ccgtcgcgcg
     1321 tcgtcttgct gacctgccat tgccagtcgg cctgcgcggc gacctgggcg aggtgggtga
     1381 cgcatagtac ctggcgctcg ccccccagtt cgcgcagcaa acggccgacg atctcggcga
     1441 caccaccgcc gatgccgaca tcgacctcgt cgaaaatcag cgtcggcacg ctggccgagc
     1501 gcgaggtcag tacctggatg gccaggctga tgcgcgacag ttcaccaccg gaagccacct
     1561 tggccaacgg cctggcgtcg ttgccggcga gaccgccgat gcggaactcg acctgttcga
     1621 gaccgtagac cgcgggattg tcgagcgccg gcaaggcaat ttcgaaacgc cccgaggaca
     1681 gcgccaggcg gcgcatgatc t
//
LOCUS       DAOSDX010000697         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23812_length_1701_cov_2.321385, whole genome shotgun sequence.
ACCESSION   DAOSDX010000697 DAOSDX010000000
VERSION     DAOSDX010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23812_length_1701_cov_2.321385"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>1701
                     /locus_tag="PLN02_13280"
     CDS             104..>1701
                     /locus_tag="PLN02_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOI53348.1"
                     /translation="MVSGAVALVAEQFPWMSNKNLAVTVLTTANRAANPDVEWGRGLL
                     DVGKAIRGPGLFEEDFAANLPAGYTSIFSNDIGYRPGLNGGLIKLGGGTLALTGMSSY
                     TGNTVVDGGTLVVNGSLLSPVTVGTAGTLRGTGNLSGAVTVNGVLAPGDPVGELTVGG
                     NLTMGPTARFHTGVASASAFGQLDVVGSAALPDDARIVVQVLGPGYNTAGRLEDVIRA
                     GTLIGNGSFSVTDNSLLFNFSASQDGNTVDLLVRHDASVNLQEMVSNAGKRSARSAAR
                     VIDDELAGNPASRLGGMFGGFTDGQESQLAQAISQTLPLLSGETSRVAQSVQGYVGRI
                     VQTRRHDPAGLPENSSVEGDREMWLRPFGTWAEQKDRGDVPGYDLSTGGMVLGTEAAV
                     SAASRLGLAFAYASSRLQGGAAGASHRAEMLGYQLLGYGSHALDADSELSFQAGFGRN
                     ATDGRRHIPFASGTALAKYHSLTASAGIGWERRFQLDEKTSFLPSIRADYRWVRESAY
                     REKGPATLSPLLLDVAGRVSEELML"
BASE COUNT          300 a          524 c          580 g          297 t
ORIGIN      
        1 cgacgttccc ggcctgccgc tatccagcac cggcattctc ggcgccgaca gcacggcgca
       61 ggagggttat tttcacgcca cggggacgtc cgccgccgcg cccatcgtat ccggcgcggt
      121 ggcgctggtc gccgagcagt ttccttggat gagcaacaag aatcttgcgg tgaccgtcct
      181 caccaccgcc aaccgagcgg ccaacccgga tgtcgaatgg ggacgcggcc tgcttgatgt
      241 gggcaaggcg attcgcggac ccggcttgtt cgaagaggac ttcgcggcga atctcccggc
      301 gggctacacc tcgatcttca gcaacgacat cggctaccgt cccggactga atggcgggct
      361 gatcaagctc ggcggcggca ccctggcgct gaccggaatg tccagctata ccggcaatac
      421 cgtcgtcgac ggcggcacgc tggtggtcaa tggcagcctg ctcagcccgg tcaccgtggg
      481 tacagccggc acgctgcgcg gcacgggcaa cttgtcgggg gccgtgacgg tgaacggcgt
      541 gctggcgccc ggcgacccgg tgggcgagct tacggtgggt ggcaatttga cgatgggccc
      601 cacggccagg tttcataccg gggtcgcaag cgctagcgct tttggccagc tggatgtcgt
      661 cgggagcgcg gcgttgccgg acgacgccag aatcgtcgta caggtactgg gccccggtta
      721 caacacggcg ggcaggctgg aggacgtcat ccgggcggga acgctgatcg gcaatggtag
      781 tttcagcgtc accgacaatt ccctgctgtt caacttttcc gccagccagg acggcaatac
      841 cgtcgacctg ctggtgcggc acgatgcttc cgtaaatctg caggaaatgg tgagcaatgc
      901 cggcaagcgc tcggcgaggt cggcggccag ggtcatcgat gatgaacttg ccggcaatcc
      961 ggcgagccgg cttgggggaa tgttcggggg cttcacagat gggcaggaaa gccagcttgc
     1021 gcaagccatc agccagacgc tgccgctgct gagcggcgaa accagccgcg tcgcccagtc
     1081 ggtgcagggt tacgtcggac gcatcgtcca gacgcgtcga catgatcccg caggcttgcc
     1141 ggaaaattcg tcggtcgaag gcgaccggga aatgtggctg cggccgttcg gtacctgggc
     1201 cgagcagaag gatcgcgggg atgttcccgg ctatgacctc agtaccggcg gcatggtgct
     1261 cggcaccgag gcagcggttt ccgccgccag ccgtcttggc ctcgcttttg cctacgccag
     1321 cagccgcctg caaggcggag cagcgggcgc ttcccaccgc gcggagatgc tcggctatca
     1381 gttgctcggc tatggcagtc atgccctgga tgccgatagc gaactgagct ttcaggcagg
     1441 attcgggcga aatgccaccg atggtcggcg tcacattcca ttcgcctccg gtacggcgct
     1501 tgccaagtat cacagcctga cggccagcgc cgggataggt tgggaaagaa gattccagct
     1561 cgacgaaaaa acgagcttcc tgccgtcgat tcgtgccgat taccgctggg tcagggagag
     1621 cgcctaccgg gaaaaggggc cggccacgct atcgccgctg ttgctcgacg tcgccgggcg
     1681 tgtttcggag gaactgatgc t
//
LOCUS       DAOSDX010000698         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23853_length_1699_cov_2.324209, whole genome shotgun sequence.
ACCESSION   DAOSDX010000698 DAOSDX010000000
VERSION     DAOSDX010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23853_length_1699_cov_2.324209"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLN02_13285"
     CDS             complement(<1..368)
                     /locus_tag="PLN02_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HOI53349.1"
                     /translation="MHGVSLKQLLTEIVDHYGFEILYAYLNINCFNTKPSIDSSVKFL
                     KKTDWAREKVEAFYLYQFKGLPRASAEQFELPPRDRIVPPDQQPGLPAELSLEDAERL
                     REKRARKAAAPDRDAGQRNGP"
     gene            complement(554..644)
                     /locus_tag="PLN02_13290"
     tRNA            complement(554..644)
                     /locus_tag="PLN02_13290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(608..610),aa:Ser,seq:gga)
     gene            complement(708..>1699)
                     /locus_tag="PLN02_13295"
     CDS             complement(708..>1699)
                     /locus_tag="PLN02_13295"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOI53350.1"
                     /translation="EIRVTRNAKRVLREKRVDLQRAWSETSFRMQAMRDNPDCAQQEF
                     DRILDVADPGLTPKITFDPQEDFTQVYRDISGRPRVAILREQGVNSHYEMAAAFDRAG
                     FQSVDVHMSDILAGRVSLKDFKGLVACGGFSYGDVLGAGQGWAKTILMHDGCRDEFAA
                     FFNRPDTFALGVCNGCQMMSALKSIIPGAEHWPAFRRNQVEQFEARFVMTEVLDSPSI
                     FFAGMEGSQIPIVVSHGEGRAVFDNADDQGQCLSAVRYVDNKGEPTEVYPYNPNGSPG
                     GLTAVTTADGRFTIMMPHPERVFRSVQMSWHPENWGEDSPWMRMFRNARRWVG"
BASE COUNT          308 a          506 c          559 g          326 t
ORIGIN      
        1 gggccgttgc gctggccggc gtcccggtcg ggcgccgcag ccttccgggc gcgcttttcc
       61 cgcaagcgct cggcatcctc caggctcaat tcggcaggca ggccgggctg ctgatccggc
      121 ggcacgatgc gatcccgcgg cggcagttca aactgctcgg cagaggcgcg cggcaggccc
      181 ttgaattgat acaggtagaa ggcctcgacc ttttcccggg cccagtccgt cttcttgagg
      241 aacttgacgc tggaatcgat gctcggcttg gtattgaagc aattgatgtt caagtaggcg
      301 tacaggatct cgaagccgta gtgatcgacg atttccgtca gcagttgctt caggctgacg
      361 ccatgcaagg ggttgttctt gtaatcgatg tcagcagtca tgggcctgga tcagcgggga
      421 tggcgttttc atggggaaac aagcttgcta cagtagcgcg gcgcgcagcc tctgaccaac
      481 agcttgtctt gctcaactgc aaaacaaaaa gcccttggtg tccaagggct tgaattgctt
      541 gctgcagaat tattggcgga gagggcggga ttcgaacccg cggtgggcta ttaacccaca
      601 cacgctttcc aggcgtgcga cttaaaccgc tcatccacct ctccggaagc cgcgcattgt
      661 agcagaagcg cggcgccggt caaaccgatt tgccgaaatt tccgggatca acccacccag
      721 cggcgggcgt tgcggaacat ccgcatccac gggctgtcct ctccccagtt ttccgggtgc
      781 caggacatct gcacgctgcg gaagacgcgc tccgggtgcg gcatcatgat cgtgaaccgg
      841 ccgtcggccg tggtcaccgc agtcaggcca cccggcgaac cgttcgggtt gtacggataa
      901 acctcggtcg gctcgccctt gttgtcgacg tagcgcacgg cggacaggca ctggccctgg
      961 tcgtcggcat tgtcgaacac ggcgcggcct tcgccgtgcg agacgacgat ggggatctgc
     1021 gagccttcca tgccggcgaa gaagatcgac ggcgaatcga gcacttcggt catcacgaag
     1081 cgcgcttcga actgctcgac ctggttgcgg cggaaagccg gccagtgttc ggcaccgggg
     1141 atgatggact tgagtgcgct catcatctgg cagccgttgc agacgccgag cgcgaaggtg
     1201 tccggacggt tgaagaaggc ggcgaactcg tcgcgacagc cgtcgtgcat caggatggtc
     1261 ttggcccagc cctggccggc gccgagcacg tcgccgtagg agaagccacc gcaggcgacg
     1321 aggcccttga agtccttgag gctgacgcgg ccggcgagga tgtcgctcat atgcacgtcg
     1381 accgactgga agccggcacg gtcgaaggcg gcagccattt cgtaatggct gttgacgccc
     1441 tgctcgcgca ggatggcgac gcgcggacgg ccgctgatgt cgcgatagac ctgagtgaaa
     1501 tcttcctgcg gatcgaaggt gatcttcggc gtcaggccgg gatcggcaac gtcgaggatg
     1561 cggtcgaatt cctgctgcgc gcaatccggg ttgtcgcgca ttgcctgcat gcgaaagctg
     1621 gtttccgacc aagcacgctg caggtcaaca cgcttctcgc gcagcacgcg cttggcgttg
     1681 cgggtgacgc ggatttcgt
//
LOCUS       DAOSDX010000699         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23914_length_1694_cov_2.613179, whole genome shotgun sequence.
ACCESSION   DAOSDX010000699 DAOSDX010000000
VERSION     DAOSDX010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23914_length_1694_cov_2.613179"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLN02_13300"
     CDS             complement(<1..438)
                     /locus_tag="PLN02_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI53351.1"
                     /translation="MSQPPTAGQEYQRPRKGIATLDEIAGFDTIIDTRSPAEFADDHL
                     PGAISCPVLDNAQRAEIGTLYKQVSPFAAKKLGAAYVSANIARHLQENFLDRDKGWRP
                     LIVCWRGGMRSGAMTTVFRSVGWDACQLEGGYKAFRHHVISEIG"
     gene            501..1556
                     /gene="selD"
                     /locus_tag="PLN02_13305"
     CDS             501..1556
                     /gene="selD"
                     /locus_tag="PLN02_13305"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOI53352.1"
                     /translation="MENQDTTPALRLTSFAHGGGCGCKISPVTLNQILAHVGPGVIPP
                     QLLVGIESSDDAAVYQLNERQAIVATTDFFTPIVDDPYDFGRIAATNALSDVYAMGGT
                     PILALALVGMPLDKLPLEVIGRIIEGGASVCREAGIPVAGGHSIDVVEPIYGLVALGI
                     VDPAKVRKNAAAQAGDVLVLGKPLGIGVLSAALKQGKLSADGYAEMLRWTTLLNTPGP
                     ALAEIDGVHALTDVTGFGLAGHLLEICRGSRLGAAVNFADLPLLASAVAFARDGVATG
                     ASTRNWQSYGSEVTLPADFPLWQQKLLTDPQTSGGLLVSCAADAVDAVLACFREKGFA
                     EATVIGRMVEGSGLRVG"
     gene            complement(1575..>1694)
                     /locus_tag="PLN02_13310"
     CDS             complement(1575..>1694)
                     /locus_tag="PLN02_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOI53353.1"
                     /translation="LLGSTVVLLFPKGRLAFNPDWAPTRPVRMGEAMASALTP"
BASE COUNT          259 a          587 c          577 g          271 t
ORIGIN      
        1 cccgatctcg gaaatgacgt ggtgccgaaa cgccttgtag ccgccttcca gttggcaggc
       61 gtcccagccg accgagcgga agacggtggt catcgccccc gagcgcatgc cgccgcgcca
      121 gcagacgatc agcggccgcc agcccttgtc gcggtcgagg aagttttcct gcagatggcg
      181 ggcgatgttg gccgagacat aggcggcgcc gagcttcttg gcggcaaacg gcgacacctg
      241 cttgtacagg gtgccgatct cggcgcgctg cgcattgtcg agcaccgggc aggaaatcgc
      301 gcccggcagg tggtcgtcgg caaactcggc gggcgagcgg gtgtcgatga tggtgtcgaa
      361 accggcgatc tcgtccagcg tggcgatgcc cttgcggggg cgttgatatt cctggccggc
      421 ggtgggcggc tgcgacatct tccgttatcc ttcgcggcgt tcgagaaaaa acggcatttt
      481 aaccagcctc ggtgcgcaac atggaaaacc aagacacgac cccggccctg cgcctgacca
      541 gcttcgccca cggcggcggc tgcggttgca agatttctcc ggtcaccctc aaccagatcc
      601 tcgcccacgt cggccccggc gtgattccgc cgcaactgct ggtcggcatc gaatcgtccg
      661 acgacgccgc cgtctaccag ttgaacgagc ggcaggcgat cgtcgccacc accgacttct
      721 tcacccccat cgtcgacgac ccctacgact tcggccgcat cgccgcgacc aacgcgctgt
      781 ccgacgtcta tgccatgggc ggcacgccga tcctggcgct ggcgctggtc ggcatgccgc
      841 tcgacaagct gccgctcgag gtcatcggcc gcatcatcga aggcggcgcc agcgtctgcc
      901 gcgaagccgg catcccggtc gccggcggtc attcgatcga cgtcgtcgaa ccgatctacg
      961 gcctggtcgc gctcggcatc gtcgatccgg ccaaggtgcg caagaacgcc gcggcgcagg
     1021 ccggcgacgt gctggtgctg ggcaagccgc tcggcatcgg cgtcctctcg gcggcgctca
     1081 agcaaggcaa gctttcggcc gacggctacg ccgagatgct gcgctggacg acgctgctca
     1141 acacgccggg accggcgctg gcggagatcg acggcgtgca tgcgctgacc gacgtcaccg
     1201 gcttcgggct ggccggccac ttgctggaaa tctgccgcgg ctcccggctc ggcgccgcag
     1261 tgaacttcgc cgatctgccg ctgctcgcca gcgccgtcgc cttcgcccgc gacggcgtcg
     1321 ccaccggcgc gtcgacgcgc aactggcaga gctacggcag cgaagtgacg ctgccggccg
     1381 atttcccgct ctggcagcag aagttgctga ccgatccgca gacctccggc ggcctgctgg
     1441 tcagttgcgc ggcggacgcg gtcgatgccg tgctcgcctg tttccgggaa aaggggtttg
     1501 ccgaagcgac cgtgatcggc cgcatggtcg agggcagcgg cctgcgcgtc ggctgagcgc
     1561 cgctcgggcg tcgcttacgg cgtcagcgcg ctggccatcg cttcgcccat gcgtaccggg
     1621 cgcgtcggcg cccagtccgg gttgaaggcg aggcggccct tgggaaagag caggacgacc
     1681 gtcgagccga gcag
//
LOCUS       DAOSDX010000700         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23970_length_1691_cov_2.109413, whole genome shotgun sequence.
ACCESSION   DAOSDX010000700 DAOSDX010000000
VERSION     DAOSDX010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23970_length_1691_cov_2.109413"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /gene="lysS"
                     /locus_tag="PLN02_13315"
     CDS             <1..1175
                     /gene="lysS"
                     /locus_tag="PLN02_13315"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOI53354.1"
                     /translation="KGWDVGDIIAVEGGLTRTKTGELSVKATALRLLTKSLRPLPDKF
                     HGLADQEMKYRQRYVDLIMNEETRFTFRARSAIVSSIRNYMCGHGFMEVETPMMHPIP
                     GGAAAKPFVTHHNALDMQQFLRIAPELYLKRLVVGGFEKVFEVNRNFRNEGLSPRHNP
                     EFTMMEFYEAYANYHTLMDFTEGLIRHAAREALGKEVFIYQGRELDLSKPFHRLTITQ
                     AIQREQPSFTDEQLADAEFLKAKIVAFGEKVKPGGLGSLQLQLFEACAEAQCWEPTFI
                     IDYPVEVSPLARASDSNPEITERFELFIVGREIANGFSELNDPEDQAARFQEQAKAKD
                     AGDEEAMYYDADYIRALEYGLPPTGGCGIGIDRLVMLLTDSPAIRDVILFPQMRPE"
     gene            1304..>1691
                     /locus_tag="PLN02_13320"
     CDS             1304..>1691
                     /locus_tag="PLN02_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53355.1"
                     /translation="MQIRQLQVARDAIQDRLILRVATQANEEYRVWITRRYLRELWPH
                     LTAMLAGHLAARPRQASEAPAKAAQASFEQPFKDDNPLYPLGSTPLLASEATLAEAGE
                     GLARLTFREGRERAFNLNLNAELMQAL"
BASE COUNT          325 a          584 c          508 g          274 t
ORIGIN      
        1 tcaagggctg ggacgtgggc gacatcatcg ccgtggaagg cggtctgacc cgcaccaaga
       61 ccggcgagct gtcggtcaag gccaccgcgc tgcgcctgct gaccaagtcg ctgcgcccgc
      121 tgccggacaa gttccatggc ctggccgacc aggaaatgaa gtaccgccag cgctacgtcg
      181 acctgatcat gaacgaggaa acgcgcttca ccttccgcgc ccgttcggcg atcgtctcgt
      241 cgatccgcaa ctacatgtgc ggccacggct tcatggaagt cgagacgccg atgatgcacc
      301 cgatccccgg cggcgccgcg gccaagccct tcgtcaccca ccacaacgcg ctcgacatgc
      361 agcagttcct gcgcatcgcg ccggagttgt acctgaagcg cctggtcgtc ggcggtttcg
      421 agaaggtctt cgaggtcaac cgcaacttcc gcaacgaagg cttgagcccg cgccacaatc
      481 ccgaattcac gatgatggag ttctacgagg cgtatgcgaa ctaccacacg ctgatggact
      541 tcaccgaagg cctgatccgc cacgccgcgc gcgaagcgct gggcaaggaa gtcttcatct
      601 accaggggcg cgaactcgac ctgtccaagc ctttccatcg cctgaccatc acccaggcga
      661 tccagcgcga gcaaccgtcg tttaccgacg aacaactggc cgacgccgaa ttcctcaagg
      721 ccaagatcgt cgccttcggc gagaaggtca agccgggcgg tctcggcagc ctgcaactgc
      781 agctgttcga agcctgcgcc gaagcgcagt gctgggagcc gaccttcatc atcgactacc
      841 cggtcgaagt cagcccgctg gcccgcgcct ccgacagcaa cccggaaatc accgagcgct
      901 tcgagctgtt catcgtcggc cgcgagatcg ccaacggatt ctccgagctt aacgacccgg
      961 aagaccaggc cgcccgcttc caggaacaag ccaaggccaa ggatgccggc gacgaggaag
     1021 cgatgtacta cgacgccgac tacatccgtg cgctcgaata cgggctgccg ccgaccggtg
     1081 gctgcggcat cggcatcgac cggctggtca tgctgctcac cgacagcccg gcgatccgcg
     1141 atgtcatcct cttcccccaa atgcgccccg aataaaccat ggctgcgctt gccataacgg
     1201 cgttgcaacg gcacttgcat agcgctgcta tgcggcgtgc cgttgcgcct tgttctggct
     1261 gcgctcgctc atggtttgcg ccgaagcggc accaggccaa ctgatgcaga tccgccaact
     1321 ccaggttgcc cgcgacgcta tccaggatcg cctgatcctg cgcgtagcga cgcaggcgaa
     1381 cgaggaatac cgggtctgga tcacccggcg ctatctgcgc gagttgtggc cgcacctgac
     1441 ggcaatgctc gccggccacc tggccgcccg gccgcggcaa gcgtccgagg cgccggcgaa
     1501 ggcggcgcag gcgagtttcg agcagccgtt caaggacgac aacccgctct atccgctcgg
     1561 ttcgacgccg ctgctggcca gcgaggcgac gctggccgag gcgggtgaag gcctggctcg
     1621 cctgactttc cgcgaaggtc gcgaacgtgc cttcaacttg aatctcaacg ccgaactgat
     1681 gcaggcgctg t
//
LOCUS       DAOSDX010000701         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_23980_length_1690_cov_2.262385, whole genome shotgun sequence.
ACCESSION   DAOSDX010000701 DAOSDX010000000
VERSION     DAOSDX010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23980_length_1690_cov_2.262385"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..495)
                     /gene="nuoE"
                     /locus_tag="PLN02_13325"
     CDS             complement(22..495)
                     /gene="nuoE"
                     /locus_tag="PLN02_13325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOI53356.1"
                     /translation="MFSADTLQKFAREVAKYPADQKQSAVMACLAHAQEEKGWLPPEA
                     IEAVADYLGMPPIAAYEVASFYNMYDLKPVGKYKITVCTNLPCALSGGYHAGEYLQQK
                     LGIGYNETTADGKFTLKEGECMGACGDAPVFIVNNRSMCSHMHPEQIDKLLEECK"
     gene            complement(495..>1690)
                     /locus_tag="PLN02_13330"
     CDS             complement(495..>1690)
                     /locus_tag="PLN02_13330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOI53357.1"
                     /translation="VLRLVLELESEVVQRADPHIGLLHRGTEKLAETRTWVQSVSYMD
                     RLDYVSTMCNEHAYCLGVEKLLQIEVPERGKYIRTMFDEITRLLNHLLWIGCHALDVG
                     AMTMALYTFREREDLMDAYEAVCGARLHAAYYRPGGVYRDLPDRMPQYRESTWTSAKK
                     AKELNDNRSGSLLDFIEDFTERFPKYHSEYHTLLTDNRIWKQRLVNVGVVSPERAMQL
                     GFTGAMLRGSGVAWDLRKKQPYAAYDKVDFDIPVGVNGDSYDRYLVRMEEMLQSNRII
                     KQCVDWLRKNPGPVIVDNHKVAPPKREAMKSNMEELIHHFKLFTEGIHVTPGEAYAAI
                     EHPKGEFGIYFISDGANKPYRMKIRAPGFAHLAAMDEMATGHMLADVVTIIGSQDIVF
                     GEIDR"
BASE COUNT          305 a          504 c          525 g          356 t
ORIGIN      
        1 tgatcgcccc catcatggcc attacttgca ctcctcgagc agcttgtcga tctgttccgg
       61 gtgcatgtgg ctgcacatcg aacggttgtt cacgatgaac accggcgcgt cgccgcaggc
      121 ccccatgcac tcgccttcct tgagcgtgaa tttgccgtcg gcggtcgttt cgttgtagcc
      181 gatgccaagc ttctgctgca ggtattcgcc ggcgtgatag ccgccggaca gggcgcacgg
      241 caggttggta cagaccgtga tcttgtactt gccgaccggc ttgaggtcgt acatgttgta
      301 gaaggaagcc acctcgtagg ccgcgatcgg cggcatgccg aggtagtcgg caacggcctc
      361 gatcgcttcc ggcggcagcc agcccttctc ttcctgggca tgggccagac aggccatgac
      421 cgccgactgt ttctgatcgg cggggtactt ggcgacctcg cgggcgaatt tctgtagggt
      481 gtcagcggaa aacatcagcg gtcaatctcg ccaaaaacga tatcctggga accgatgatc
      541 gtcacgacgt cagccagcat gtggccggtt gccatttcgt ccatcgccgc cagatgggca
      601 aagccgggcg cacgaatctt catgcggtaa ggcttgttgg cgccatcgga aatgaaatag
      661 atgccgaact cgcccttcgg atgctcgatc gccgcatagg cttcgccggg cgtgacatgg
      721 atgccttcgg tgaacagctt gaagtggtgg atcagttctt ccatgttcga cttcatcgct
      781 tcgcgcttcg gcggcgccac cttgtggtta tcgacgatca ccgggccggg gttcttgcgc
      841 agccagtcga cgcactgctt gatgatgcgg ttcgactgca gcatctcttc catgcggacg
      901 aggtaacggt cgtagctgtc gccgttgacg ccgaccggaa tgtcgaaatc gaccttgtcg
      961 taggcagcgt aaggctgctt tttgcgcaaa tcccaagcga cgccggaacc gcgcagcatg
     1021 gcgccagtga agccgagctg catggcgcgc tccggactga cgacaccgac gttgaccaga
     1081 cgctgcttcc agatccggtt gtcggtcagc agggtgtggt attccgaatg gtatttcggg
     1141 aagcgctcgg tgaagtcctc gatgaaatcg agcagcgagc cgctgcggtt gtcgttgagt
     1201 tccttcgcct tcttggcact ggtccaggtc gactcgcgat attgcggcat gcgatccggc
     1261 agatcgcgat agacaccgcc cggccggtag taggccgcgt gcagacgggc gccgcagacc
     1321 gcctcgtagg cgtccatcag gtcttcgcgc tcgcggaagg tatagagcgc catggtcatc
     1381 gcgccgacgt cgagcgcgtg acagccgatc cacaacaggt gattgaggag acgggtgatc
     1441 tcgtcgaaca tcgtccggat gtacttgccg cgctccggta cttcgatctg caacagcttt
     1501 tcgacgccga ggcagtaggc gtgctcgtta cacatcgtcg acacgtagtc gagacgatcc
     1561 atgtacgaca ccgactgcac ccaggtgcgg gtttcagcga gcttctcggt gccccggtgc
     1621 agcaggccga tgtgcggatc ggcgcgctgg acgacttcgc tctccagttc cagcaccaga
     1681 cgcaatacgc
//
LOCUS       DAOSDX010000702         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24021_length_1687_cov_3.728554, whole genome shotgun sequence.
ACCESSION   DAOSDX010000702 DAOSDX010000000
VERSION     DAOSDX010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24021_length_1687_cov_3.728554"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLN02_13335"
     CDS             complement(<1..1233)
                     /locus_tag="PLN02_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI53358.1"
                     /translation="MRALLRPFLRLLVGLLFRVDVQFRQSDFSQRKLLIVANHESFLD
                     GLLLGLFLPLDPVFVVHTGVANNFWFRLLLSLVDYLAVDPTSPMAMKKVIRLIESGRP
                     VVIFPEGRITRTGSLMKVYDGPAFVAAKTGATVIPVRVDGASRSYFSRLSGSYPKVMF
                     PKIRLTVLPEAHFPMPEAPTAKQRRRKSGEQMRKLMQEMIFASRPNQTLYGALCDAAR
                     VYGRGRRLLEDVKQIEYSYNDLLKMALILGRQIERVSRPGEKVGLLLPNLVPTIGLIF
                     GLTARKRVPALLNYTAGTDAMQAACTAAEVKTVVTSRAFVEQAKLGDKLAGLQGVELL
                     YLEDIRERIGLADKLWLVLWAIRFPHAFELHASPEDAAVVLFTSGSEGKPKGVVLPHR
                     AILANIAQIRSVIDFSVHD"
     gene            complement(1233..1544)
                     /locus_tag="PLN02_13340"
     CDS             complement(1233..1544)
                     /locus_tag="PLN02_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOI53359.1"
                     /translation="MKTDYSQTIPPQWAVMSKIFVALGDEHRQRILLLFEPGERLNVG
                     QIAEVSTLARSTVSHHLKVLHEAGVLASEKIGKEVWFWIDRPLLETTFTNVLDYLREF
                     T"
BASE COUNT          356 a          526 c          531 g          274 t
ORIGIN      
        1 gtcgtgtacc gagaaatcga tcaccgaacg aatctgcgcg atgttggcga gaatcgcccg
       61 gtgcggcagc acgacgccct tcggcttgcc ttcggagccc gaggtgaaca gcaccaccgc
      121 tgcgtcctcc ggcgaagcat gcagttcgaa tgcgtgcgga aagcggatcg cccacagcac
      181 cagccacagc ttgtcggcga ggccgatgcg ttcgcggatg tcttcaagat agagtaattc
      241 gacgccctgc agcccggcca gcttgtcgcc gagcttggcc tgctcgacga aggcgcgcga
      301 ggtcaccacc gtcttcacct cggcggcggt gcaggcggcc tgcatggcgt cggtgccggc
      361 ggtgtagttg agcagcgccg gcacgcgctt ccgggcggtg aggccgaaga tcaggccgat
      421 ggtcggcacc aggttgggca gcagcaggcc gaccttctcg cccggccgcg acacgcgctc
      481 gatctgccgg ccaagaatca gcgccatctt cagcaggtcg ttgtacgaat actcgatctg
      541 cttcacatcc tccagcaggc gccggccgcg cccatacacc cgggccgcgt cgcacagcgc
      601 gccgtacagc gtctggttcg ggcgcgaagc gaagatcatc tcctgcatca gcttgcgcat
      661 ctgctcgccg gacttgcgcc gacgctgctt ggcggtgggc gcctcgggca tcgggaaatg
      721 cgcttccggc agcacggtca ggcgaatctt cggaaacatc accttcgggt aactgcccga
      781 caggcgcgag aagtaggagc gcgaagcgcc atcgacgcgc accgggatca ccgtcgcgcc
      841 ggtcttggcg gcgacgaagg cggggccgtc gtagaccttc atcagcgaac cggtgcgggt
      901 gatccgccct tccggaaaga taaccaccgg ccgcccggat tcgatcagcc ggatcacttt
      961 cttcatcgcc atcgggctgg tcggatcgac cgccaggtaa tcaaccagcg acagcagcaa
     1021 acggaaccag aaattgttgg cgacgccggt atgcacgacg aaaaccggat cgagcggcag
     1081 aaacaggcca agcagcaaac cgtcgaggaa ggactcgtgg ttggcgacga tcaacagctt
     1141 gcgctgcgag aaatcggact gccggaactg cacatcgacg cgaaacagca ggccgaccag
     1201 cagccgcagg aaaggacgca gcaaggcacg catcaggtga actcccgaag gtaatcgagg
     1261 acgttggtaa aggtggtttc cagcaacggg cggtcgatcc agaaccacac ctccttgccg
     1321 atcttctccg aagccagcac cccggcctcg tgcagaacct tcaggtgatg cgacacggtc
     1381 gaccgcgcca gcgtcgacac ctcggcaatc tgcccgacat tgagccgctc acccggctcg
     1441 aacaacagca ggatgcgctg ccgatgctcg tcgcccaagg caacgaaaat cttggacatg
     1501 acggcccact gggggggaat ggtttggctg tagtcggtct tcatgtcgat aagtttagtt
     1561 atgtcgatat gatttagtca agggaaaatt tggggagtgc catatcgaga gggatgccac
     1621 cgatctgagc ccggatgatc ggcgacgaat ccgctggaca tgtgataacc acatgcagcg
     1681 cgcaagc
//
LOCUS       DAOSDX010000703         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24024_length_1687_cov_3.197917, whole genome shotgun sequence.
ACCESSION   DAOSDX010000703 DAOSDX010000000
VERSION     DAOSDX010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24024_length_1687_cov_3.197917"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLN02_13345"
     CDS             complement(<1..315)
                     /locus_tag="PLN02_13345"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOI53360.1"
                     /translation="MNGNSGCVIWTITDGKPGHLQQIRGLLKALRQQLPVREIELRAL
                     PPLQALAHLAGGSFPPGEGLPVPDLVVGAGHATHLNILAAKRAYGGKTVVLMKPSLPR
                     QLF"
     gene            complement(423..1283)
                     /locus_tag="PLN02_13350"
     CDS             complement(423..1283)
                     /locus_tag="PLN02_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOI53361.1"
                     /translation="MTTLSMILVACLAGGLISMAAAALVVFRLSRQSTAILVAFAAGV
                     MLSSAFLHVLPEAFNALPPVDIHAGHDHAPDDQAAIAAEAAADHEHEHEGHDHGGPFA
                     TLFALMLGTLFAFFALERLALWRHSHGDCADHEGHHATTTAPPLILIGDAFHNFVDGV
                     LIAAAFIADPMLGVTTTVAIIAHEVPQEMGDFVLLRNAGWSNTKAFVANAGSSLSSLL
                     GGALGFFALSTADFVLPYVLVIGASSFIYVALADLFPLLHKQGESFFRQILLIAAGVA
                     VVPLVGMFAH"
     gene            complement(1280..>1687)
                     /locus_tag="PLN02_13355"
     CDS             complement(1280..>1687)
                     /locus_tag="PLN02_13355"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HOI53362.1"
                     /translation="KGVDLKRLQDADPKASELLAKVRKEWDKAPVERSLNGQRIRIPG
                     FVVPLERKGDDILEFLLVPYFGACVHVPPPPSNQVILVVPAKPVRGMRTMETFTVSGT
                     LTLQGGDSGMGIYAYRLAAERVEPYQVPQGKKK"
BASE COUNT          307 a          525 c          587 g          268 t
ORIGIN      
        1 gaaaagttgg cgcggcaggc tgggcttcat cagcaccacc gtcttgccgc cgtaggcgcg
       61 cttggcggca aggatgttga ggtgagtggc gtgaccggcg ccgacgacca ggtcggggac
      121 cggcagccct tcaccgggcg gaaagctgcc gccggccaga tgggccagcg cctgcagcgg
      181 cggcagcgcg cgcagttcga tctcgcgcac cggcaactgc tggcgcagcg ccttgagcaa
      241 gccccgaatc tgctgcagat ggccgggctt gccgtcggtg atggtccaga tgacacagcc
      301 ggaattgccg ttcatgccgt tttcgctcct gaatttgtcc cggtgggacg cgcggagcta
      361 ttgtcggcag gctcggtgac aaagccgtta aagctggata acgcctatgt gacgcttggg
      421 cttcagtggg caaacatccc gaccagcggc acgacggcga cgccagcggc gatcagcagg
      481 atctgccgga aaaaactctc gccctgcttg tgcagcagcg gaaagaggtc ggccagcgcg
      541 acgtagatga agctcgaggc gccgatcacc agcacgtaag gcagcacgaa atcggcggtg
      601 gacagcgcga agaaaccgag cgctccgccg agcagcgaac tcaggctgga gccggcgttg
      661 gcgacgaagg ccttggtgtt gctccagcca gcgttgcgca ggagcacgaa atcgcccatt
      721 tcctgcggca cctcgtgagc aatgatggcg accgtcgtgg tcacgccgag catcgggtcg
      781 gcgatgaagg cggcggcgat cagcacgcca tcgacgaagt tgtggaaggc atcgccgatc
      841 aggatcagcg gcggcgccgt ggtcgtcgcg tggtgtccct catggtcggc gcaatccccg
      901 tgcgaatgcc gccacagcgc cagacgctcc agggcgaaaa acgcgaacag cgtgcccagc
      961 atcagcgcga acagcgttgc gaacggaccg ccgtggtcgt gcccctcgtg ctcgtgctca
     1021 tggtcggcgg cggcctcggc ggcgattgcc gcctggtcgt cgggcgcatg gtcgtggccg
     1081 gcatggatat cgaccggcgg caaggcattg aaggcttccg gcagaacgtg caggaaggcc
     1141 gacgacaaca tgacgccggc ggcaaaggca acgaggatcg ccgtcgattg ccgcgacagg
     1201 cggaacacca ccagcgccgc ggcggccatg ctgatcagcc cgccggcgag gcaggcgacg
     1261 aggatcatgc tcagggtagt catttcttct ttccttgtgg gacctgatag ggttcgaccc
     1321 gctcggcggc gagacggtag gcatagatgc ccatgccgct gtcgccgccc tgcagcgtca
     1381 gggtgccgct gaccgtaaag gtctccatgg tgcgcatgcc gcgcaccggt ttggccggca
     1441 ccaccaggat gacctggttc gacggtggcg gcggaacatg gacgcaggcg ccgaaatacg
     1501 gcacaagcag gaattcgagg atgtcgtcgc ccttgcgttc gagcggcacg acgaacccgg
     1561 ggatgcggat gcgctggccg ttcaggctgc gctcgaccgg cgccttgtcc cattccttgc
     1621 gcaccttggc gagcagttcg ctggccttgg gatcggcatc ctgcagccgc ttcagatcga
     1681 cgccctt
//
LOCUS       DAOSDX010000704         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24043_length_1686_cov_2.576334, whole genome shotgun sequence.
ACCESSION   DAOSDX010000704 DAOSDX010000000
VERSION     DAOSDX010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24043_length_1686_cov_2.576334"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /gene="argB"
                     /locus_tag="PLN02_13360"
     CDS             <1..787
                     /gene="argB"
                     /locus_tag="PLN02_13360"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOI53363.1"
                     /translation="GGNAMTDEHLKSCFARDVVLLELIGFNIVVVHGGGPQIENLLTR
                     VGKKGEFIQGMRVTDAETMEVVEMVLGGQVNKDIVNLINQHGGKAVGLTGKDGNCIRA
                     KKLMLEDKDHPGDLIDVGQVGEITKIDPSLIDHLDKGAFIPVIAPIGVGEEGETYNIN
                     ADVVAGKIAEVLKAEKLVLLTNTPGVLDKAGNLITGITPKEIDEMVADGTLSGGMLPK
                     IGSALDAARNGVKGVHIIDGRVEHALLLEILTDHGVGTMIKSH"
     gene            791..1432
                     /locus_tag="PLN02_13365"
     CDS             791..1432
                     /locus_tag="PLN02_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOI53364.1"
                     /translation="MRGERVWLFDLDNTLHNASPHIFPHINRAMREYIERHLGLDENA
                     ANRLRQDYWQRYGATLLGLIRHHAIDPHHFLHETHQFPDLARMLVFEKPLLHALKRLP
                     GRKILFSNAPRAYAEAVLELTGLDRHFAAVYSVESLKFRPKPMPQGFRTLLHAERLDP
                     RRCIMVEDSLANLVTARKLGMKTVWVSAGSRRSPYVDVQVSSVLQLPQRCARL"
     gene            1467..>1686
                     /gene="slmA"
                     /locus_tag="PLN02_13370"
     CDS             1467..>1686
                     /gene="slmA"
                     /locus_tag="PLN02_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HOI53365.1"
                     /translation="MATKPGERRLQILQTLAEMLETPKGEKITTAALAGKLECSEAAL
                     YRHFASKAQMFEGLIEFIEQSLFGVINQI"
BASE COUNT          360 a          574 c          488 g          264 t
ORIGIN      
        1 cggcggcaac gcgatgaccg acgagcacct gaaaagctgc ttcgcccgcg acgtcgtcct
       61 gctcgaactg atcggcttca acatcgtcgt cgtgcacggc ggcggcccgc agatcgagaa
      121 cctgctcacc cgcgtcggca agaagggcga gttcattcag ggcatgcgcg tcaccgacgc
      181 cgaaaccatg gaagtcgtcg aaatggtgct cggtggccag gtcaacaagg acatcgtcaa
      241 cctgatcaac cagcacggcg gcaaggctgt cggcctgacc ggcaaggacg gtaactgcat
      301 ccgcgccaag aagctgatgc tggaagacaa ggaccacccg ggcgacctga tcgacgtcgg
      361 ccaggtgggg gaaattacca agatcgaccc gtcactgatc gaccacctcg acaagggtgc
      421 gttcatcccg gtaatcgccc cgatcggcgt cggcgaggaa ggtgaaacct acaacatcaa
      481 cgccgacgtc gtcgccggca agatcgccga agtgctcaaa gccgaaaagc tggtgctgct
      541 gaccaatacc ccgggcgtgc tcgacaaggc cggcaatctg atcaccggca tcacgcccaa
      601 ggagattgac gagatggtcg ccgacggcac gctgtccggc ggcatgctgc cgaagatcgg
      661 ctcggcgctc gatgctgccc gcaacggcgt caagggcgtg catatcatcg acggccgcgt
      721 cgaacacgcg ctgctgctcg aaatcctgac cgaccacggg gtcggcacga tgatcaagtc
      781 gcactaaagc atgcgtgggg aacgcgtctg gttgttcgac ctcgacaaca cgctgcacaa
      841 cgcttccccg cacatcttcc cgcacatcaa ccgggcgatg cgcgagtaca tcgagcgcca
      901 cctcggcctc gacgaaaacg ccgccaaccg gctgcgccag gactactggc agcgctacgg
      961 cgcaaccctg ctcggcctga tccgccacca cgcgatcgac ccgcatcatt tcctgcacga
     1021 aacccatcag tttcccgatc tcgcccgcat gctggtattc gagaagccgc tgctgcacgc
     1081 gctcaagcgc ctgcctgggc gcaagatcct gttctccaac gcgccgcgcg cctacgccga
     1141 ggcggtgctc gaactgaccg gactggatcg ccacttcgcc gcggtgtatt ccgtcgaaag
     1201 cctgaaattc cggcccaagc cgatgccgca gggcttccgc accctgctgc acgccgagcg
     1261 cctcgacccg cgccgctgca tcatggtcga ggacagcctg gccaacctcg tcaccgcccg
     1321 caagctcggc atgaaaaccg tgtgggtgag cgctggctct cgccgttcgc cctacgtcga
     1381 cgtccaggtt tcgtcggtcc tgcaattgcc gcaacgctgc gctcgtctat aatccgggca
     1441 caaaaattct tcgagggaca acatcgatgg cgaccaaacc gggcgaacgc cgcctgcaga
     1501 tcctgcaaac cctggctgaa atgctggaaa ccccgaaagg ggaaaagatc accaccgccg
     1561 ccctggccgg caagctggaa tgctcagaag ccgcgctcta ccgtcacttc gccagcaagg
     1621 cgcagatgtt cgaggggctg atcgagttca tcgaacagag cctgttcggc gtcatcaacc
     1681 agatca
//
LOCUS       DAOSDX010000705         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24140_length_1680_cov_3.137846, whole genome shotgun sequence.
ACCESSION   DAOSDX010000705 DAOSDX010000000
VERSION     DAOSDX010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24140_length_1680_cov_3.137846"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLN02_13375"
     CDS             complement(<1..466)
                     /locus_tag="PLN02_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53366.1"
                     /translation="MSRILIVDDEASILKALQRLLRVAPCTYGNRRFTLEVHTATSPL
                     HALELARHEEFDLFISDYRMPEMDGIEFLKHAKAVQPDAARLILSGYADLNALVRAVN
                     EVGIDRFIGKPWNDYDLMSAIGQALAHRELLLENRQLADLVRVEMGDKTPEQV"
     gene            complement(463..>1680)
                     /locus_tag="PLN02_13380"
     CDS             complement(463..>1680)
                     /locus_tag="PLN02_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI53367.1"
                     /translation="ILTAEGGAAGLVIMEGEAVNLVITDIRMPEMDGATFLKQVRERW
                     PQTKRILLTGYADITSTVSAINEGEIYRYIAKPWDDKEMLAVVREACEHQRLEQENQR
                     LTELTQAQNEALKTLNASLEQKVAERTAELSQALTFVEQAHGELKKSFMTSVQVFAGL
                     IELRSGPLGKQLAGHGRRVAELARSLARHLGLAESDAQNILLAGLLHDIGKLGLPDEL
                     LGRPFNMLSAEHRAQVMKHPVVGQNILLGIERFKEAAVLVRHHHESYDGSGYPDHLAA
                     MAIPQGARILAVANDYDALQVGTLVQRPLKPAEALDFLIENRGKRYDPAVVDALVRLV
                     AETVKGGPQEIPLRTMHLKPGMTVSRDLMHRDGYLLLAAGSVLNSDIIGQLIRLEQVE
                     QQNLTVYIRQEEK"
BASE COUNT          246 a          489 c          598 g          347 t
ORIGIN      
        1 cgacctgttc cggcgtcttg tcgcccatct cgacgcgcac caggtcggcg agctggcggt
       61 tttccagcag cagctcacgg tgcgccagcg cctggccgat cgccgacatc aggtcgtaat
      121 cgttccacgg cttgccgatg aagcggtcga tgccgacttc gttgaccgcc cgcaccaggg
      181 cgttgaggtc ggcgtagccg gagaggatca ggcgcgccgc gtcgggctgc acggccttgg
      241 cgtgcttgag gaactcgatg ccgtccatct ccggcatccg gtaatcgctg atgaagaggt
      301 cgaattcttc atggcgggca agttccaggg cgtgcagcgg cgaggtcgcg gtgtgcacct
      361 cgagcgtgaa gcgacggttg ccgtaggtgc agggagcgac ccgcaacagg cgttgcagcg
      421 ccttgaggat cgatgcttcg tcgtcgacga tcaggatgcg gctcattttt cctcctggcg
      481 gatataaacg gtgagatttt gctgctcgac ctgttccaga cggatcaact ggccgatgat
      541 gtcgctgttg agcacgctgc cggcggcgag cagcaggtaa ccgtcgcggt gcatcaggtc
      601 gcggctgacc gtcatgccgg gtttcaggtg catggtgcgc agcgggattt cctgcgggcc
      661 gcccttgacg gtttcggcga ccagcctgac cagcgcgtcg accactgccg ggtcgtagcg
      721 cttgccgcga ttctcgatca ggaagtcgag tgcctcggcc ggtttcagcg ggcgctgcac
      781 cagggtgccg acctgcagcg cgtcgtaatc gttggcgacg gcgaggatgc gggcaccctg
      841 cggaatcgcc atggcggcga gatggtccgg gtagccgctg ccgtcgtagg actcgtggtg
      901 gtggcggacg agcaccgcgg cttccttgaa gcgttcgatg ccgagcagga tgttctggcc
      961 gaccaccggg tgtttcatta cctgggcacg gtgctcggca ctcagcatgt tgaacgggcg
     1021 gccgagcagt tcgtcgggta ggccgagctt gccgatgtcg tgcagcaggc cggcgagcag
     1081 gatgttctgg gcgtcgcttt cggccaggcc gaggtggcgg gcgaggctgc gcgccagttc
     1141 ggcgacgcgc cggccgtggc cggccagttg cttgccgagc gggccgctgc gcagttcgat
     1201 caggccggcg aagacctgga cactggtcat gaaggatttc ttcagttcgc catgcgcctg
     1261 ctcgacgaaa gtcagcgcct ggctgagttc ggcggtgcgt tcggcaacct tctgttcgag
     1321 gctggcgttg agggtcttca gggcttcgtt ctgggcctgg gtgagttcgg tcagacgctg
     1381 gttttcctgt tccaggcgct ggtgttcgca ggcttcccgg acgacggcga gcatttcctt
     1441 gtcgtcccag ggcttggcga tgtagcggta gatttcgcct tcgttgatcg ccgagacggt
     1501 agacgtgatg tcggcgtagc cggtgagcag gatgcgcttg gtctgcggcc agcgttcacg
     1561 aacctgtttg aggaaggtgg cgccgtccat ttccggcatg cgaatgtcgg taatcaccag
     1621 attcaccgct tcgccttcca tgatgacgag gccggcggca ccgccttcgg cggtcaggat
//
LOCUS       DAOSDX010000706         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24187_length_1677_cov_3.011097, whole genome shotgun sequence.
ACCESSION   DAOSDX010000706 DAOSDX010000000
VERSION     DAOSDX010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24187_length_1677_cov_3.011097"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PLN02_13385"
     CDS             <1..171
                     /locus_tag="PLN02_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOI53368.1"
                     /translation="SAIRTDCDGDVLLLTVEQVGGIACHTGRASCFFNELQGERWVPV
                     DPVLKDPKDIYQ"
     gene            168..497
                     /locus_tag="PLN02_13390"
     CDS             168..497
                     /locus_tag="PLN02_13390"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOI53369.1"
                     /translation="MSTHDILHRLSETLASRRHADPEKSYTAKLFSEGPDSILKKIGE
                     ECAELIMAGKDGKRLNIVRESTDVVYHILVLLAFYGLSIEDVSQEMRRREGISGIDEK
                     ASRSANK"
     gene            494..844
                     /locus_tag="PLN02_13395"
     CDS             494..844
                     /locus_tag="PLN02_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOI53370.1"
                     /translation="MSDCLFCKIVAGQIPAQKVYEDELVLAFRDIHPQRPVHILVIPK
                     KHITSLATAAADDADILGRMLVKANEIAVAEGSPNGFRVIINTGEVGQQEVPHLHMHI
                     VGGPDPVGPMLKRI"
     gene            854..1084
                     /gene="tatA"
                     /locus_tag="PLN02_13400"
     CDS             854..1084
                     /gene="tatA"
                     /locus_tag="PLN02_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HOI53371.1"
                     /translation="MGSFSIWHWLIVLVIVMLVFGTKKLRNVGQDLGGAVKGFKDGMK
                     EANADKPAEGAQPTQQVGGQTIDVEVKEKTRS"
     gene            1139..1513
                     /gene="tatB"
                     /locus_tag="PLN02_13405"
     CDS             1139..1513
                     /gene="tatB"
                     /locus_tag="PLN02_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOI53372.1"
                     /translation="MFDIGFSELMVIGIVALLVIGPERLPKVARTLGHLLGRAQRYVN
                     DVKSDINREMQLDELRKLQAQVSESARSLESSVRQELDAVERSVSTPVQAALDDLQAG
                     ATKLEQDVAVPVTAGDRSDKAA"
BASE COUNT          336 a          501 c          529 g          311 t
ORIGIN      
        1 agcgccatcc gcaccgactg cgatggcgac gtgctgctgc tgacggtcga gcaggtcggt
       61 ggcattgcct gccataccgg ccgggctagc tgctttttca acgagttgca aggggaacgc
      121 tgggttcccg tcgatccggt tttgaaagat ccgaaggaca tttaccaatg agcacgcacg
      181 acatcctgca tcgcctgtcc gaaaccctgg cgtcgcgccg tcacgccgac ccggaaaaat
      241 cctacacggc caagctgttc tccgaaggtc cggactcgat cctgaagaag atcggcgagg
      301 aatgcgccga gctgatcatg gccggcaagg acggcaagcg cctgaacatc gttcgcgaat
      361 cgaccgacgt cgtctatcac atcctggtcc tgctcgcctt ctacggcttg tcgatcgagg
      421 acgtgtcgca ggaaatgcgc cgccgtgaag ggatttccgg catcgacgag aaggcctcgc
      481 ggagcgccaa caagtgagcg actgcctgtt ctgcaagatt gtcgccggac agattccggc
      541 gcagaaggtg tacgaggacg agttggtgct cgccttccgc gacattcatc cgcagcggcc
      601 ggttcatatt cttgtcatcc cgaagaagca tattacgtcg ttggccacag ccgctgccga
      661 cgatgccgac attcttggcc ggatgctggt caaggccaac gagatcgcgg ttgccgaagg
      721 cagccccaac ggttttcgtg tcatcatcaa cacgggtgag gtggggcagc aggaagtgcc
      781 ccatctgcac atgcacatcg tgggcggccc cgatccggtc ggtcccatgc tcaagcgaat
      841 ctaggagaca actatgggta gcttttccat ctggcactgg ctgatcgtcc tggtcatcgt
      901 catgctcgtt ttcggtacca agaaactgcg caatgtcggc caggacctcg gcggtgcggt
      961 caagggcttc aaggacggca tgaaggaagc gaatgccgac aaaccggcgg aaggcgcgca
     1021 gccgacgcaa caggtcggtg gtcagaccat cgacgtcgaa gtcaaggaaa agacccggag
     1081 ctaagcgggc tggtgatggc gcggagataa cgcggcatcg tctggcacca atcttcccat
     1141 gttcgatatc ggcttttccg aactgatggt gatcgggatc gtggccttgc tggtcatcgg
     1201 tcccgaacgc ctgcccaagg tcgcgcgcac gctcgggcat ttgctcggtc gggcccagcg
     1261 ctacgtcaac gacgtcaagt ccgacatcaa tcgcgaaatg cagctcgatg aattgcgcaa
     1321 gctgcaggcg caggtcagcg aatcggcgcg ctcgctcgaa agctcggtgc gccaggagct
     1381 ggacgccgtg gagcgtagcg tatcgacgcc cgtgcaggcg gcgctcgatg acctgcaggc
     1441 gggagcgacc aagctggagc aggatgtcgc tgtgccggtg acggctggcg acaggagcga
     1501 caaggcagca tgagcgaaac cctgcaggaa gaatccttca tctcgcatct ggtcgaactg
     1561 cgcgaccgcc tgttgcgcag cctgatcgcg attgccgtcg tgatgggggc gctggcgctc
     1621 tatcccggcc cgggcgccat ctacgatttt ctcgccgcgc cgctaaccag cgctctg
//
LOCUS       DAOSDX010000707         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24206_length_1676_cov_2.936459, whole genome shotgun sequence.
ACCESSION   DAOSDX010000707 DAOSDX010000000
VERSION     DAOSDX010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24206_length_1676_cov_2.936459"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLN02_13410"
     CDS             complement(<1..264)
                     /locus_tag="PLN02_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOI53373.1"
                     /translation="MRIALYVTCLVDLMRPAVGFAALRLLEATGNDVVVPPAQTCCGQ
                     PAWSAGNRPLAMELARKAIAELESYDVVVVPSGSCAEQIKSVYP"
     gene            395..649
                     /locus_tag="PLN02_13415"
                     /pseudo
     CDS             395..649
                     /locus_tag="PLN02_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238108.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     assembly_gap    650..659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <660..1110
                     /locus_tag="PLN02_13420"
     CDS             <660..1110
                     /locus_tag="PLN02_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOI53374.1"
                     /translation="ARHDDEIVYLERTSSGRSLVRVVYLVGGRAPLHLTSLGKLFLAA
                     ESAQKVRDYARRTGLPGKTPHSLTTLLALEKELDKVRRHGIAYDDEEAELGLKCVAAP
                     IHDDEGNVVAALSVSAPADRHDPEWGRQVKETAEEVSRALGYIKPRQ"
     gene            complement(1159..>1676)
                     /locus_tag="PLN02_13425"
     CDS             complement(1159..>1676)
                     /locus_tag="PLN02_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOI53375.1"
                     /translation="GTTMTRETAMLLALMSSENRAAHALGRHYPGGMRAFVDAMNRKS
                     RALGMFDTRFEEPTGLSSNNVSTAHDLARMVAAAVRYPQIRMFSTTAEAKVELNGRIQ
                     QFGNTNSLVRSDNWQIGLSKTGYISEAGRCLVMQAWVAGKPVVIVLLDSQGKATRVGD
                     ANRIKRWMESA"
BASE COUNT          319 a          581 c          499 g          267 t
ORIGIN      
        1 gggatacacc gacttgatct gttcggcgca ggaacccgag ggcacgacga cgacatcgta
       61 actttccagc tcggcaatcg ccttgcgtgc cagctccatc gccagcggcc ggttgccggc
      121 actccaggcg ggttggccgc agcaggtctg ggccggcggc acgacgacgt cgttgccggt
      181 tgcttcaagc aggcgcaagg cagcaaagcc caccgccgga cgcatcaggt ccaccaggca
      241 ggtaacgtat aaggcgatcc tcatgttaat attgcgccgc accacaaggt aaagtggcca
      301 atgctactca aaccccgcga ataaaggaat ccagttgtca gaagttgccg acggcgccac
      361 ccggacgcct ggttcgatcc aggtcatcga gcgcatgatg tccttgcttg acgcgcttgc
      421 cgaagccccg gacgccgcga gtctgaaaca cctggccgcc gttaccgaac tgcacccatc
      481 gacggcgcat cgcatcctcg ccgcgatgtg caacgcccgc ttcgtcgaac gccaggatgc
      541 cggcaactac cgcctcggca tccggctgct cgaacttggc aacatcgtca agtcgcgcat
      601 caacctgcgc gaagtcgcgc tgcctttcat gcagaccctg catgaaaaan nnnnnnnnnc
      661 gcccgccacg acgacgagat cgtctacctc gaacgcacct cgtccggccg ctcgctggtc
      721 cgcgtcgtct acttggttgg cggtcgcgcc cccctgcacc tgacctcgct cggcaagctc
      781 ttcctggccg ccgaaagcgc ccagaaagtt cgcgactacg cccgccgcac cggcctcccc
      841 ggcaagacgc cgcactcgct gaccaccttg ctcgccctcg agaaggaact cgacaaggtg
      901 cgccggcacg gcatcgccta cgacgacgag gaagccgaac tcgggctgaa atgcgtcgcg
      961 gcgccgatcc acgacgacga gggcaacgtc gtcgcagccc tctcggtatc ggccccggcc
     1021 gaccgccacg acccggaatg gggccggcag gtcaaggaga cggccgagga ggtctcgcgc
     1081 gctctcggct acatcaagcc gcgccaatga aaaaggcggc cagcggccgc ctttctgctt
     1141 atcccggaga ataaaagctc aggcgctttc catccagcgc ttgatccggt tggcgtcgcc
     1201 aacccgggtc gccttgccct gcgaatcgag cagcacgatg accaccggct tgccggcaac
     1261 ccaggcctgc atcaccagac agcgcccggc ttccgagatg taaccggtct tcgacagacc
     1321 gatctgccag ttgtcgctgc gcaccagcga attggtgtta ccgaactgct ggatgcgacc
     1381 gttcagttca accttggcct cggccgtcgt cgagaacatg cgaatctgcg ggtaacgaac
     1441 cgcggcggcg accatgcgcg ccaggtcgtg ggcggtggaa acgttgttgc tggagaggcc
     1501 ggtcggctcc tcgaaacggg tgtcgaacat gccgagcgcg cgcgacttgc ggttcatcgc
     1561 gtcgacgaag gcgcgcatgc cgccaggata gtggcgcccg agggcgtgcg ccgcccgatt
     1621 ctcggaagac atcagcgcca gcagcatcgc cgtctcacgg gtcatcgtcg tcccca
//
LOCUS       DAOSDX010000708         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24272_length_1672_cov_2.967842, whole genome shotgun sequence.
ACCESSION   DAOSDX010000708 DAOSDX010000000
VERSION     DAOSDX010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24272_length_1672_cov_2.967842"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLN02_13430"
     CDS             complement(<1..530)
                     /locus_tag="PLN02_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOI53376.1"
                     /translation="MSPDQYCQEKCAKSGSSFYYSFLFLPPERRQAIMALYAFCREVD
                     DVVDECSDLSLASTKLVWWRMEVQRIAEKQATHPVGLALQAVAPGINLPSEQLLEIID
                     GMEMDLQQSRYLDFKGLSLYCYRVASVVGLLAAEIFGYQDRQTQKYAHDLGMAFQLTN
                     IIRDVGEDARRGRIYLP"
     gene            complement(535..1344)
                     /gene="hpnC"
                     /locus_tag="PLN02_13435"
     CDS             complement(535..1344)
                     /gene="hpnC"
                     /locus_tag="PLN02_13435"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOI53377.1"
                     /translation="MPVDHYENFPVASFLVPAPLRRPIEVIYRFARSADDIADEGEAT
                     PEQRLAELAAYQAELDRIAAGTRPQTPLFVDLAEVIAAHALPVQLFRDLLDAFAQDVV
                     KTRYADYPELVDYCRRSANPVGRLVLALFKRTGDTQLMQSDCICTALQLINFWQDIAI
                     DWQKDRIYLPQVEMQRFGVGEDDIASGRCGAGWEKLIDFQVDRARTLMLRGAPLVHAL
                     PGRIGWEIRLTVQGGLRILERIARLRGDVFRHRPKLGKADWFVLLVRSIRM"
     gene            1398..1482
                     /locus_tag="PLN02_13440"
     tRNA            1398..1482
                     /locus_tag="PLN02_13440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1432..1434,aa:Leu,seq:tag)
     gene            1534..>1672
                     /locus_tag="PLN02_13445"
     CDS             1534..>1672
                     /locus_tag="PLN02_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HOI53378.1"
                     /translation="MIVRPRLHWFPLLFVWRGSVLPQIAGRLLLIFAVSVGSVLGRDW
                     WM"
BASE COUNT          274 a          504 c          592 g          302 t
ORIGIN      
        1 ggcaggtaga tgcggccgcg ccgcgcgtcc tcgccgacgt cgcggatgat gttggtgagc
       61 tggaaggcca tgcccaggtc gtgcgcgtat ttctgcgtct gacggtcctg gtagccgaag
      121 atttcggcgg cgagcaggcc gacgacgctg gcgacgcggt agcagtagag cgacagcccc
      181 ttgaagtcga gatagcgcga ctgctgcagg tccatttcca tgccgtcgat gatttccagc
      241 aactgctcgc tcggcaggtt gatgccgggg gcgaccgcct gcagcgccag gccgaccggg
      301 tgagtggcct gtttctcggc gatgcgctgc acctccatcc gccaccagac cagcttggtc
      361 gaggcgagcg aaaggtcgga gcattcgtcg acgacgtcgt cgacctcgcg gcagaaggcg
      421 tacagcgcca tgatcgcctg gcggcgttcg ggcggcagga agaggaagct gtaatagaag
      481 gaggagccgc tcttggcgca cttctcctgg cagtattggt cggggctcat gggctcacat
      541 tctaatggaa cggacgagca gcacgaacca gtcggccttg cccagcttgg gccggtggcg
      601 gaagacgtcg ccgcgcaagc gtgctatgcg ttccagaatg cgcaggccgc cctgcacggt
      661 gaggcggatt tcccagccga tccgcccggg cagcgcgtgg accagcggcg ccccgcgcag
      721 catcagggtg cgcgcccggt cgacctggaa atcgatcagt ttctcccagc ccgcgccgca
      781 acggccactg gcaatgtcgt cctcgccgac gccgaaacgt tgcatctcga cttgcggcag
      841 gtagatccgg tccttctgcc agtcgatggc gatgtcctgc cagaaattga tcaactgcag
      901 cgcggtacag atgcagtcgg actgcatcag ttgcgtgtcg ccggtgcgtt tgaacaaggc
      961 cagaaccagc cggccgaccg ggttggccga gcgtcggcag taatcgacca gctccggata
     1021 gtcggcgtag cgtgtcttga ccacgtcctg ggcgaaggcg tcgagcaggt cgcggaacaa
     1081 ctggacgggc agggcgtggg cagcgatgac ttcggccagg tcgacgaaca gcggcgtctg
     1141 cggccgggtg ccggcggcga tgcggtcgag ttcggcctgg taggcggcaa gttcggccag
     1201 gcgctgctcg ggcgtcgcct cgccctcgtc ggcgatgtcg tcggcgctgc gcgcgaagcg
     1261 gtagatcacc tcgatcggcc ggcgcagcgg cgccggcacg aggaaggaag caaccggaaa
     1321 attttcgtag tgatcgacgg gcatggggcg gcaagtatag ggggcggggc gactaagtgc
     1381 tagaatgcgg cccgcctgcc caggtggcga aattggtaga cgcaccagat ttaggttctg
     1441 gcgccgcaag gcgtgggggt tcgagtccct tcctgggcac catgtttcat gcgccggtcg
     1501 cacttcgtgc gcaccggatg ccggtagtca aagatgatcg tccgcccgcg cctccattgg
     1561 tttcccctgc ttttcgtctg gcgcggttcg gtcctgccgc agattgccgg gcgcctgttg
     1621 ctgattttcg cggtctccgt cggcagtgtc ctcgggcgtg actggtggat gc
//
LOCUS       DAOSDX010000709         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24280_length_1672_cov_2.294991, whole genome shotgun sequence.
ACCESSION   DAOSDX010000709 DAOSDX010000000
VERSION     DAOSDX010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24280_length_1672_cov_2.294991"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1672)
                     /locus_tag="PLN02_13450"
     CDS             complement(<1..>1672)
                     /locus_tag="PLN02_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOI53379.1"
                     /translation="YQVNKKTLIEKIAELVNDKKIEGISDIRDESDKSGMRIVIELKR
                     GEVGEVILNNLYKQTQLQDTFGMNMVALVDGQPRLLNLKQMLECFLSHRREVVTRRTV
                     YELRKARERGHILEGLAVALSNVDEIIALIKAAPTPPDAKRGLMERTWRSPVVEEMLL
                     RAASDVSRPDGLLPEFGLSEQGYRLSDAQAQAILELRLQRLTGLEQDKIVGEYKEVMA
                     KILDLLDILARPERITEIIVGELTAVRDQFGDDRRSEIVLHTQELGIEDFITPQDMVV
                     TLSHGGYIKAQPLADYRAQKRGGRGKQAAAIKDEDFVDHLFVANTHDYILCFSNRGRC
                     YWLKVFEAPQGSRTSRGKPIVNLFPLEEGERINAVLPVKEFVDDKFIFMATRDGTVKK
                     TPLSDFSNPRKAGIIAVDLLDDDYLIGVELTTGQSDIVLVSNAGKAVWFDEEDVRPMG
                     RGARGVRGMKLQEGQTVISLLVAEDDQQTVLVATENGFGKRTVLADFRHSGRGTQGVK
                     AIADSERNGVVVGAKLVGDDDEVMLITTGGVLIRTRVSEIRGMGRATQG"
BASE COUNT          270 a          536 c          538 g          328 t
ORIGIN      
        1 accctgcgtc gcccggccca tgccgcggat ttccgagacg cgggtgcgga tcagcacgcc
       61 gccggtggtg atcagcatca cctcgtcgtc gtcgccgacc agcttggcac cgaccaccac
      121 gccgttgcgc tcggaatcgg caatcgcctt gacgccctgc gtgccgcgac cggagtgacg
      181 gaagtcggcg agcacggtgc gcttgccgaa gccattctcg gtggcgacca gcaccgtctg
      241 ctgatcgtct tcagcaacca gcagcgaaat cacggtctgt ccttcctgca gcttcatgcc
      301 gcgcacgccg cgggcaccgc ggcccatcgg ccggacgtct tcctcgtcga accagaccgc
      361 cttgccggcg ttggagacga gcacgatgtc gctctggccg gtggtcagtt cgacgccgat
      421 caggtagtcg tcgtcgagca gatcgacggc gatgatgccg gccttgcgcg ggttggagaa
      481 gtcggacaac ggcgtcttct tgaccgtacc gtcgcgggtg gccatgaaga tgaacttgtc
      541 gtcgacgaat tccttgaccg gcagcacggc gttgatgcgc tcgccttctt ccagcgggaa
      601 caggttgacg atcggcttgc cgcggctggt ccggctcccc tgcggcgcct cgaagacctt
      661 cagccagtag cagcggccgc ggttcgagaa gcacaggatg tagtcgtggg tgttggcgac
      721 aaacaggtga tcgacgaaat cctcgtcctt gatcgccgcc gcctgcttgc cgcgcccgcc
      781 gcgcttctgc gcgcggtagt cggcgagcgg ctgggccttg atgtagccgc cgtgcgacag
      841 ggtgacgacc atgtcctgcg gcgtgatgaa gtcctcgatg ccgagttcct gcgtgtgcag
      901 cacgatttcc gaacggcggt cgtcgccgaa ctggtcgcgc accgctgtca gctcgccgac
      961 gatgatctcg gtgatgcgtt ccgggcgggc caggatgtcg agcaggtcga ggatcttggc
     1021 catgacttcc ttgtactcgc cgacgatctt gtcctgctcg agaccggtca ggcgctgcag
     1081 gcgcagttcg aggatggcct gggcctgggc gtcggacaga cggtagccct gttcggacag
     1141 accaaactcc ggcagcaggc cgtccggacg cgagacgtcg gaagctgcgc gcagcagcat
     1201 ttcctcgacc accgggctgc gccaggtgcg ctccatcagg ccgcgcttgg catccggcgg
     1261 cgtcggcgcc gccttgatca gggcgatgat ttcgtcgacg ttggacagcg cgacggccaa
     1321 accttccagg atgtggccgc gttcgcgcgc cttgcgcagt tcgtacacgg tacgccgggt
     1381 gacgacttcg cggcggtgcg acaggaagca ctcgagcatc tgcttgaggt tcaagaggcg
     1441 cggctggccg tcgaccagcg ccaccatgtt cataccgaag gtatcctgca gctgcgtctg
     1501 cttgtacagg ttgttgagaa tgacctcacc gacctcgcca cgcttcagtt caatgacgat
     1561 acgcatgccc gacttgtcgg actcgtcacg aatgtcggaa atcccttcga tcttcttgtc
     1621 attgaccagt tcggcaatct tctcgatcag cgtcttcttg ttgacctgat ag
//
LOCUS       DAOSDX010000710         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24338_length_1669_cov_3.208178, whole genome shotgun sequence.
ACCESSION   DAOSDX010000710 DAOSDX010000000
VERSION     DAOSDX010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24338_length_1669_cov_3.208178"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1378)
                     /locus_tag="PLN02_13455"
     CDS             complement(56..1378)
                     /locus_tag="PLN02_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOI53380.1"
                     /translation="MEIFLLVALIVLNGVFAMSEIALVTARRARLAKQAEEGDGAAAV
                     AMQLGEDPTRFLSTIQIGITSIGILNGIVGEAALAGPLAVWLQGWGADRDLSEIGATV
                     LVVVVITYVSIVVGELVPKRIGQINPEGIARLVARPMNWLAILSRPFVLFLSNSTALL
                     LRLLGQRESRGPSVTEEEIHAMLVEGSAAGVIEKNEHDMVRNVFRLDDRQLGSLMVPR
                     SEIVWLDIKRPLDENLAIITESDHARFPVCRDGLENIIGVLNARQLLGKTLRGSTPGL
                     DEKLQPAVYVPESLTGMELLEQFRSSGTHLVFVVDEYGEVQGMVTLQDLVESVTGEFV
                     PKNKEDAWAVQREDGSWLLDGLIPVLELKDTLGLKSVPEEEKGRYHTLSGMLMLLLGR
                     LAETGDRQVWEGWTLEVVDLDGKRIDKVLATPLPEVPPATASEAEPTP"
BASE COUNT          298 a          559 c          531 g          281 t
ORIGIN      
        1 ccacgccagc gcggcgcgaa gaagccgccc ccgggggtag ccgcggcaac gccgttcacg
       61 gcgtcggctc cgcctcgctc gcggtcgccg gcggcacttc cggcaacggc gtcgccagca
      121 ccttgtcgat ccgcttgccg tcgagatcaa cgacttccag cgtccagcct tcccagacct
      181 ggcggtcgcc ggtctcggca agacggccga ggagcagcat cagcatgccg gacaaggtgt
      241 ggtagcggcc tttctcctcc tccggaacgc tcttcaagcc gagcgtgtcc ttcagttcga
      301 gcaccgggat caggccgtcg agcagccagg agccatcctc gcgctgaacc gcccaggcgt
      361 cttccttgtt cttcgggacg aactcgccgg tcaccgactc gaccaggtcc tgcagcgtca
      421 ccatgccctg cacctcgccg tattcatcga cgacgaagac gaggtgggtg ccggaactgc
      481 ggaactgttc gagcagttcc atcccggtca gcgactccgg cacatagacc gccggctgca
      541 gcttttcatc gagtccgggc gtactgccgc gcagggtctt gccgagcaac tggcgggcat
      601 tgaggacgcc gatgatgttc tccagcccat cgcggcagac cgggaaacgg gcgtggtcgg
      661 attcggtgat gatcgccagg ttctcgtcga gcgggcgctt gatgtcgagc cagacgattt
      721 ccgagcgcgg caccatcagc gaaccgagct gtcggtcgtc gaggcggaag acgttgcgca
      781 ccatgtcgtg ctcgttcttc tcgatgacgc cggcggccga gccttcgacc agcatggcgt
      841 ggatttcttc ctcggtgacg ctcgggccgc ggctttcccg ctgcccgagc aagcgcagca
      901 agagggccgt cgaattcgac aggaagagga cgaaaggccg cgacaggatg gccaaccagt
      961 tcatcggtcg cgccaccagg cgggcgatgc cttcggggtt gatctggccg atccgcttcg
     1021 gcaccagttc gccgacgacg atcgagacat aggtgatgac gacaacgacc agcaccgtgg
     1081 cgccgatttc gctgaggtcc cgatcggccc cccagccttg caaccagacg gcgagcggcc
     1141 cggcgagcgc ggcctcgccg acgatgccgt tgaggatgcc gatcgaggtg atgccgatct
     1201 ggatcgtgga caggaagcgg gtcgggtctt cgcccaactg cattgctacc gcggcggcac
     1261 cgtcgccctc ttcggcctgc ttggcaagac gggcgcgacg ggcggtgacg agggcgattt
     1321 cggacatcgc gaaaacgccg ttgaggacaa tcagcgcgac cagtagaaag atttccatgt
     1381 cagtagcctc cgccaacggc aaacaaggaa attgcgatgt tcggtaaaac gggggaatgc
     1441 tccatgctgc ggccccagag ggcgccgccc agcctttctt gtgttgcgga cagagcggat
     1501 catgccgcaa ttcggcgacg cttgccatcg tccgccgccc gcctcgccgg cgccgaccga
     1561 tcgataaaac cgaagtcttt gctcgaagaa agcccggcgc ttgccaaagt catggcaacg
     1621 ccgccccaag ccccgccttg acgcctccgg cctccatttg ctcacgcag
//
LOCUS       DAOSDX010000711         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24382_length_1667_cov_2.944169, whole genome shotgun sequence.
ACCESSION   DAOSDX010000711 DAOSDX010000000
VERSION     DAOSDX010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24382_length_1667_cov_2.944169"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..490
                     /gene="ndhC"
                     /locus_tag="PLN02_13460"
     CDS             116..490
                     /gene="ndhC"
                     /locus_tag="PLN02_13460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOI53381.1"
                     /translation="MMEAYFPILMFVLVGVAVGVLPVAMGFLLGPRKPDAEKLSPYEC
                     GFEAFEDARMKFDVRYYLIAILFILFDLEVAFLVPWATIFKDVVATDAIKVFGFVEML
                     IFVAILTVGYVYAWAKGALEWE"
     gene            500..976
                     /locus_tag="PLN02_13465"
     CDS             500..976
                     /locus_tag="PLN02_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOI53382.1"
                     /translation="MAIEGILQEGFVTTTADKLINYMRTGSIWPMTFGLACCAVEMIH
                     AGCSRYDLDRFGIVFRPSPRQSDVMIVAGTLTNKMAPALRKVYDQMAEPRWVVSMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLIQLQNKIRRTNTIAR"
     gene            996..1601
                     /locus_tag="PLN02_13470"
     CDS             996..1601
                     /locus_tag="PLN02_13470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOI53383.1"
                     /translation="MSAKLEALSQKLQERFGEKLQSTKLALGELTVEVAAVDYFEFMK
                     SLRDEADFSFEELIDLCGVDYSTYGDGAWTGRRFAAVTHLLSIAHNFRLRVRVFAEDD
                     AFPAVDSVTSLWKSANWFEREAFDLYGIAFVGHEDLRRILTDYGFIGHPFRKDFPISG
                     HVEMRYDPDQGRVVYQPVTIEPRENTPRIVREDTYGDPAHG"
BASE COUNT          293 a          471 c          510 g          393 t
ORIGIN      
        1 gagggggtag tggcgaaagc tgtgcgagtt cgagtctcgc cgtcggcacc aaaagcgttg
       61 ggaatttccg gcgggccggg agtttcctat cggaacaacg acaatttggc ggcggatgat
      121 ggaagcgtac ttcccaattc tgatgttcgt tctcgtgggg gttgctgtcg gcgtattgcc
      181 ggtggcgatg ggttttttgc ttgggccgcg caagcccgat gcggaaaaat tgtccccgta
      241 tgagtgcggt tttgaggcct tcgaagatgc gcgcatgaag ttcgatgtgc gctactacct
      301 gatcgccatc ctgttcattc tcttcgatct ggaagtcgcc ttccttgtgc cgtgggcgac
      361 gatcttcaag gatgtcgtgg cgaccgacgc catcaaggtg ttcggtttcg tcgagatgct
      421 gattttcgtc gccatcctga ccgtgggcta tgtctatgcc tgggccaagg gcgcgctgga
      481 atgggaataa ggtcgagaga tggcgattga aggaatcctg caggaaggtt ttgtcaccac
      541 cacggctgac aagctgatca actacatgcg cactgggtcg atctggccga tgactttcgg
      601 cctggcctgc tgcgccgtcg aaatgattca cgccggttgt tcgcgctacg accttgatcg
      661 tttcggtatc gtattccgtc ccagtccgcg tcagtcggac gtgatgatcg tcgccgggac
      721 gctgaccaac aagatggcgc cggcgctgcg taaggtctac gaccagatgg cggagccgcg
      781 ttgggtggtt tccatggggt cctgcgccaa cggtggcggc tactaccatt attcctactc
      841 ggtggtacgt ggctgtgacc ggattgttcc ggttgatatc tacgtgccgg gctgtccgcc
      901 gaccgccgag gcgctgctct acggtctgat ccagttgcag aacaagatcc gccgcaccaa
      961 caccattgcc cgctaatttt cgaggctgat tccttatgtc tgccaagctg gaagcgctta
     1021 gccaaaaact gcaggaacgt ttcggggaaa agctgcagtc gacgaagctt gccctgggcg
     1081 agttgaccgt ggaagttgcc gcggtcgact atttcgagtt catgaagtcg ttgcgcgacg
     1141 aggccgattt ttccttcgag gaattgatcg acctctgcgg cgtcgattac tcaacctacg
     1201 gcgatggcgc ctggacgggg cgtcgctttg ccgccgtcac ccatctgctg tccatcgcgc
     1261 acaatttccg tctgcgggtt cgcgtcttcg ccgaagacga tgctttcccg gcggtcgatt
     1321 cggtgacttc gctgtggaag agtgccaact ggttcgagcg cgaggccttc gatctgtacg
     1381 ggattgcctt cgttgggcac gaggatctgc ggcgcatcct gaccgactac ggcttcatcg
     1441 gtcacccctt ccgcaaggac tttccgattt ccgggcatgt cgagatgcgc tacgaccccg
     1501 atcagggtcg cgtcgtctat cagcccgtca ccatcgaacc gcgtgaaaac acgccgcgca
     1561 tcgtccgcga agatacctac ggagatccgg cacatggctg atatccgcaa tttcaccctg
     1621 aacttcggtc cgcagcaccc ggccgcccac ggcgtgctgc gcctggt
//
LOCUS       DAOSDX010000712         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24415_length_1665_cov_3.072050, whole genome shotgun sequence.
ACCESSION   DAOSDX010000712 DAOSDX010000000
VERSION     DAOSDX010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24415_length_1665_cov_3.072050"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /gene="flgA"
                     /locus_tag="PLN02_13475"
     CDS             <1..440
                     /gene="flgA"
                     /locus_tag="PLN02_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOI53384.1"
                     /translation="VRCLAPVEWSILVPVQISVIADYVVTRKALLAGRSIQSDDLAFN
                     RGDLAALPPGTLLRPEQALGKTLRNSIGAGHPLRANQLRSPLMVRQGQAVRVIARGKG
                     FEAKAEGKALNNAAAGEVVRVRMSSGKTLGGVVQEDGSILLSN"
     gene            504..782
                     /gene="flgM"
                     /locus_tag="PLN02_13480"
     CDS             504..782
                     /gene="flgM"
                     /locus_tag="PLN02_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOI53385.1"
                     /translation="MKIDTSTFSAASVRSGGSPAKSGSANRSGNAVSLSAASTAPLDS
                     PQAPPVNAAKIQEIKQAIAQGRFEINPEAIADGLLETARDLVNSQRKA"
     gene            782..1246
                     /locus_tag="PLN02_13485"
     CDS             782..1246
                     /locus_tag="PLN02_13485"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOI53386.1"
                     /translation="MSTLRELLLQEKELLDRFITCLHEEATALKDIRPEALDAIGQSK
                     LALTQQLDTVENRRNALTSVPGSATGRAAMQQWLSRHPEEAASNALWTEVLALATEAK
                     RLHNLNGELIAMHLSKTSEAIEILSQRLRDKPLYGSDGQSTGSTGCRIVDSA"
     gene            complement(1276..>1665)
                     /locus_tag="PLN02_13490"
     CDS             complement(1276..>1665)
                     /locus_tag="PLN02_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOI53387.1"
                     /translation="IEINDSILFALGQANLQPASISALQAVAAVLADSDFPITIEGHT
                     DNVPIATPQFPSNWELSAMRATTVLRLFNDAGVGAERLTAIGYGDTRPVETNTTVEGR
                     ARNRRVSILIDSERPERPTELSAQPLQ"
BASE COUNT          339 a          554 c          493 g          279 t
ORIGIN      
        1 gcgtgcgctg cctggcgccg gtcgaatgga gcatcctggt cccggtacag atttcggtga
       61 tcgccgacta tgtcgtcacg cgcaaggcgc tactcgccgg cagaagcatc cagagcgatg
      121 acctggcgtt caaccgcggc gatctcgccg ccctgccgcc tggcaccctg ctcaggcccg
      181 agcaggcgct gggcaagacc ctgcgcaact cgatcggcgc cggccatccg ctacgcgcca
      241 accagttgcg ctcgccgttg atggtccgcc agggacaggc ggtgagagtc attgcgcgag
      301 gcaagggctt cgaagccaag gccgaaggca aggccctcaa caatgccgcc gctggcgagg
      361 tggtgcgagt ccggatgagt tccggcaaga cgctcggtgg cgtcgtacag gaagatggct
      421 ccattctttt gtcgaattag gctaaagttc aggccagagc aaccgtaatc acgaacaaga
      481 tcaaaagcac accgggtttc atcatgaaaa tcgacacctc gaccttcagc gctgcctccg
      541 tgcgctcggg aggctcgccg gcaaaatccg gcagcgccaa ccgcagcgga aacgcagtca
      601 gcctcagcgc cgcctcgacc gcgcccctgg attcgccgca ggcgccgccg gtcaatgccg
      661 cgaagattca ggaaatcaag caggccatcg ctcaggggcg tttcgagatc aatcccgaag
      721 ccatcgccga cggtctgctc gaaaccgccc gcgatctggt caacagccag cgcaaggcct
      781 gatgtccacg ctgcgcgaac tcctgctgca ggaaaaggaa ctgcttgacc ggttcatcac
      841 ctgtttacat gaggaagcca cggccctcaa agatatccgt ccggaagcgc tggacgctat
      901 cggccagtcg aaactggcac tgacccagca actcgacacc gtcgaaaatc ggcgcaacgc
      961 cttgacctcc gtccctggca gcgccaccgg acgtgccgcg atgcagcaat ggctgagccg
     1021 ccatcccgag gaagcggcga gcaatgcttt atggaccgaa gttctcgccc tggccaccga
     1081 ggccaagcgg ctacacaatc tgaacggcga attgatcgcc atgcacctga gcaagaccag
     1141 cgaagccatc gagattctct cccagcggct gcgcgacaaa ccgctctatg gcagcgacgg
     1201 gcaaagcacc ggcagcaccg gctgccgtat cgtcgactcg gcctgacgcc gtttccgccc
     1261 cacccatact cgcatttatt gcagcggttg cgccgataac tcggttgggc gctccggacg
     1321 ttccgaatcg atcaggatgc tcacacggcg gtttctcgcc cgcccttcca cggtcgtatt
     1381 cgtttcgacc ggccgggtgt cgccgtaacc gatcgccgtc agccgctcgg cgccaacccc
     1441 ggcatcgttg aacaggcgca gcacggtagt tgcccgcatc gccgacaact cccagttgga
     1501 ggggaactgc ggcgtcgcga tcggcacgtt gtcggtatgg ccttcgatgg tgatcggaaa
     1561 atccgaatcc gccagcactg ccgccaccgc ctgcaaggcg ctgatcgacg ccggttgcag
     1621 gttggcctga ccaagagcga aaaggatgct gtcgttgatt tctat
//
LOCUS       DAOSDX010000713         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24476_length_1662_cov_1.931549, whole genome shotgun sequence.
ACCESSION   DAOSDX010000713 DAOSDX010000000
VERSION     DAOSDX010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24476_length_1662_cov_1.931549"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1459
                     /locus_tag="PLN02_13495"
     CDS             146..1459
                     /locus_tag="PLN02_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HOI53388.1"
                     /translation="MKKSLRKLLPLLVAASFGTAYAATEADVENTFNPYKAGMPSFAG
                     LKAGMTINKGNVDQFKDILGAGVFKLIKEGMFEMKVGPTTQFSISKAYIDATRANLNK
                     TQLGAKPGDMISGYVAGRPFPEEPDAKDPRAGEKLAWNYKYGVNWGDGAIISPFYWQY
                     RNMQTGKVEKEINWDFHFLNFMHRTKDAPVPEITPNPSGIFRAIYVKAHEPQDLKNTQ
                     LLIQRFEDDAKLDDAYLYLGFQRRVRRLAQGQATDSFLGSDLMIEDFEGYNGRVSDMK
                     WTYKGTRNVMLPMWNHNELKLSAERTESDGYKYVDFAGQGGCFAEGTWQLRKVYVLEA
                     VPVNPNHPVSKRVFYMDVQLQNLNGSNEIYDRKGELWKVFMVGKSHADHHLPVNKGAG
                     IGIDDAFSMVDVQSKHCTTGQFKGQVDPKKNPPRLFQVQNLRGGD"
BASE COUNT          360 a          529 c          480 g          293 t
ORIGIN      
        1 cccttcacca gttattcccg tggcctggcc ggcatggaac cgctcggccg cttccgcgcc
       61 ggtccgatcg gtgcggcatt caaggaaaac gacctgttcg tcaagctgaa ctacaagttc
      121 tgaacccaac cggaggagac atttcatgaa aaaatcacta cgcaaactgc tgcccttgct
      181 cgtcgccgcc agcttcggca ccgcctacgc ggcgaccgaa gccgacgtcg agaacacctt
      241 caatccctac aaggccggca tgcccagctt cgccggcttg aaggcgggga tgacgatcaa
      301 caagggcaat gtcgaccagt tcaaggacat cctcggtgcc ggcgtcttca agctgatcaa
      361 ggaaggcatg ttcgaaatga aggttgggcc gaccacccag ttcagtattt ccaaggccta
      421 catcgacgcc actcgcgcca acctcaacaa gacccagctc ggcgccaagc cgggcgacat
      481 gatcagcggc tacgtcgccg gccgcccctt cccggaagag ccggatgcca aggacccgcg
      541 tgccggcgag aagctcgcct ggaactacaa gtacggcgtg aactggggtg acggcgcgat
      601 catctcgccg ttctactggc agtaccgcaa catgcagacc ggcaaggtgg aaaaggaaat
      661 caactgggac ttccacttcc tcaacttcat gcaccgcacc aaggacgcgc cggtgccgga
      721 aatcaccccg aacccgtcgg gcatcttccg cgccatctac gtcaaggcgc atgagccgca
      781 ggatctgaag aacacgcagt tgctgatcca gcgtttcgaa gacgatgcca agctcgacga
      841 cgcctacctc tacctcggct tccagcgccg tgtgcgccgc ctggcgcagg gccaggcgac
      901 cgactccttc ctcggctccg acctgatgat cgaagacttc gaaggctaca acggtcgcgt
      961 ttccgacatg aagtggacct acaagggcac caggaacgtc atgctgccga tgtggaatca
     1021 caacgaattg aagctgtccg ccgagcgtac cgagtccgac ggctacaagt atgtcgattt
     1081 cgccggtcag ggcggttgct tcgccgaagg cacctggcag ctgcgcaagg tctatgtgct
     1141 ggaagcggtg ccggtcaatc cgaaccatcc ggtcagcaag cgtgtcttct acatggacgt
     1201 gcagttgcag aacctgaacg gctcgaacga aatctacgac cgcaagggcg agctgtggaa
     1261 ggtgttcatg gtcggcaagt cgcacgccga tcatcacctg ccggtgaaca agggcgccgg
     1321 gatcgggatc gacgatgcct tctcgatggt cgacgtccag tccaagcatt gcacgaccgg
     1381 tcagttcaag ggacaggtgg acccgaagaa gaacccgccc cgtctgttcc aggtccagaa
     1441 cctgcgcggc ggagactgat cccgccgtca ccaacacccc atgccgggtg tctctgcggg
     1501 agggtgccga atccggcagc ctccacactt tacgggggct gcgtgccccc atttttttta
     1561 acggaggtga acatgtccga cacgaagctt tccggaatgc gcgtgctgat cacgcaatcc
     1621 cgcgatttca tgggggcggc gctgtgcgat gtccttgccg cg
//
LOCUS       DAOSDX010000714         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24486_length_1661_cov_2.899128, whole genome shotgun sequence.
ACCESSION   DAOSDX010000714 DAOSDX010000000
VERSION     DAOSDX010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24486_length_1661_cov_2.899128"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLN02_13500"
     CDS             complement(<1..470)
                     /locus_tag="PLN02_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOI53389.1"
                     /translation="MNSSSAHAVLLVGHPNVGKSVLFHRLTGAYVNVSNYPGTTVEVT
                     RAAARFDSQVQLLDTPGVLALPSRSDDERATVRALLEEPCRCLVQVGDAKNLRRTLTL
                     TALLAELGLPMVLALNMHDEATARGVTVDVEALAEELAIPVLATVATGGEGIGEL"
     gene            complement(540..>1661)
                     /locus_tag="PLN02_13505"
     CDS             complement(540..>1661)
                     /locus_tag="PLN02_13505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOI53390.1"
                     /translation="LTACQFASSLLAEGGGGIDRAELRRRLEILQARVSHSLGWISLQ
                     APPENLAPALELLLTVRRAPNLPAAAFERDKAAAIARLETAARQPAALAAKQLALRFD
                     NYPARHPARPRPLDEQKRELQAVTLDDVRRCSSDFGGRSNLRIGVVGDLGEDEVRGFW
                     QALQSLPKATVPYERIASPTAPERIDTRDIVVALPRFPNADITGRAMLPLRDDADDYL
                     PLALAVRLLGGDAASRLRVRLREQEGLAYSVSASLSASAFDARAGLTIEASVASEQAE
                     KARDILREELARALAEGFSTAEVDAGIRNWQEQRRQSLQSEGSYSLRLLHAQLTGRDG
                     AWEAARDAGIAALTRERVNAALRRHLGQAELAWAIGKGE"
BASE COUNT          220 a          527 c          633 g          281 t
ORIGIN      
        1 agttcgccga tgccttcgcc gccggtggcg acggtggcca gcaccgggat cgccagttcc
       61 tcggcgagcg cttcgacgtc gacggtgacg ccgcgcgccg tcgcttcgtc gtgcatgttg
      121 agcgccagca ccatgggcag gccgagttcg gcgagcagtg cggtcaacgt caaggtgcgg
      181 cgcaaattct tggcatcgcc gacctggacc aggcagcggc agggttcttc gagcagcgcc
      241 cgcaccgttg cccgctcgtc gtcgctgcgc gacggcagcg ccagcacgcc cggggtgtcg
      301 agcaattgca cctgactgtc gaagcgggcg gcggcgcggg tgacttcgac ggtcgtgccg
      361 gggtagttcg aaacgttgac gtaggcgccg gtcaggcggt ggaaaaggac ggatttgccg
      421 acgttgggat ggccgaccag gaggacggcg tgggcgctgg atgaattcat gcgcgtgatt
      481 ttacgctgtc accgcttttt acgcgcgtcc tggggcggcc ggagtcgggc gctcccggtc
      541 tattctccct tgccgatcgc ccaggccagt tccgcctgac ccaggtggcg gcgcagcgcg
      601 gcattgaccc gttcccgggt gagcgcggca atgcccgcgt cgcgcgcggc ttcccaggcg
      661 ccgtcgcggc cggtcagttg cgcgtggagc aggcgcaggc tgtagctgcc ttcggactgg
      721 agcgattggc gtctttgctc ctgccagttg cggatgcccg cgtcgacttc ggcggtggag
      781 aaaccttccg ccagtgctcg ggccagttcc tcgcggagaa tgtcgcgggc tttttcggcc
      841 tgctcgctgg cgacgctggc ctcgatcgtc aggccggcgc gggcgtcgaa agcgctggcg
      901 gagagcgagg cgctgacgct gtaggcgagt ccttcctgtt cgcgcaggcg aacgcgcaag
      961 cggctggccg cgtcgccgcc gagcagacgc acggccagtg ccagggggag gtagtcgtcg
     1021 gcgtcgtcgc gcagcggcag catggcccgg ccggtaatgt cggcattggg aaagcggggc
     1081 aaggcgacga cgatgtcgcg ggtgtcgatc cgctccgggg cggtcgggct ggcgatcctc
     1141 tcgtagggga ctgtggcttt cggcaaggac tgcagggctt gccagaaacc gcgtacctcg
     1201 tcttcgccga ggtcgccgac cacgccgatg cgcaggttcg agcgaccgcc gaaatcgctg
     1261 ctgcagcggc gcacgtcgtc gagggtcacg gcctgcaatt cacgcttttg ctcgtccagc
     1321 gggcgggggc gggcggggtg tcgggcgggg tagttgtcga agcgcagggc aagctgcttg
     1381 gccgccagcg cggcgggctg ccgcgcggcc gtttcgagcc tggcgatcgc cgccgccttg
     1441 tcgcgttcga aggcagctgc cggcaggttg ggcgcgcggc gcacggtcag cagcagttcg
     1501 agcgccggag cgaggttttc cggcggcgcc tgcaggctga tccagcccag gctatggctg
     1561 acccgggcct gcaggatttc gagccggcga cgcagttcgg cgcggtcgat gccgccgccc
     1621 ccctcggcca atagactgct ggcgaactgg caggcggtca g
//
LOCUS       DAOSDX010000715         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24548_length_1658_cov_2.956956, whole genome shotgun sequence.
ACCESSION   DAOSDX010000715 DAOSDX010000000
VERSION     DAOSDX010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24548_length_1658_cov_2.956956"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1575)
                     /locus_tag="PLN02_13510"
     CDS             complement(34..1575)
                     /locus_tag="PLN02_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOI53391.1"
                     /translation="MSNAFDKMSVLAVDDNRTNLHILQVFLKKLGHDVILAENGEQAV
                     QRFLKDRPDLILLDIMMPVMDGFEAARRIKAASKDKWVPIIFLSALNRDENLVEGLEA
                     GADDYLTKPINFVVLEAKLRSMQRSLALQQSAAVSLERLQAISDNVLDAIITVDCKGE
                     ILSLNRATERIFGRRIRDLLGQPVSTLLPELPSGNTATDRHGRPICALSAYKGRDRET
                     VGRRGDHADFPVEIGISEVSIDHEGLYVCIIRDISERHKAARQLQENAALLQTYYDHS
                     QNEQQLAVRLMEKLLHRPGLSDPRLRYKVTATENFSGDIVAATRSAEGRFYALLADAT
                     GHGLPAAISVLPVLALFYRMTRLNVDISEVIFELNQQLKESMPIGRFVAATLICLDEI
                     KMSGEIWIGGTPEAFLLDREGNPVLRFASENLALGIQESEQINCSPRCFTWAPGSQLL
                     CYSDGLLEAADNAGRQFGSEGVLAALRGHARECRFDRLEAALAAHLGENTAADDVSLM
                     LIDCP"
BASE COUNT          297 a          492 c          524 g          345 t
ORIGIN      
        1 cgatccggcc tttttcattt gttgctcccc cgctcagggg caatcgatca gcatcagaga
       61 aacatcgtcg gcggcggtgt tttcgcccaa gtgcgcggcc agtgcggctt ccagtcggtc
      121 gaaacggcat tctcgggcgt ggccgcgcaa cgccgccagc acgccttcgc tgccgaattg
      181 gcgacccgcg ttgtcagccg cttcgagcag cccatccgag tagcacaata gctggctgcc
      241 cggggcccag gtgaaacaac ggggcgagca attgatctgt tcgctttcct ggatgcccag
      301 tgcaagattc tccgaggcga agcgcagtac cgggttgcct tcccggtcga ggaggaaggc
      361 ttccggggtt ccgccaatcc agatctcgcc gctcatcttg atttcgtcca ggcagatcaa
      421 ggtggcggcg acgaaacggc cgatcggcat cgactccttg agctgctggt tgagttcgaa
      481 gatcacttcc gagatgtcca cgttcaggcg agtcatccgg tagaacagtg ccagaaccgg
      541 cagcacgctg attgccgccg gcaggccatg acccgtcgcg tcggcgagca gggcgtagaa
      601 gcgcccctcg gcggaacgcg tggcggcaac aatgtcgccg ctgaagttct cggtggcggt
      661 aaccttgtaa cgcagccttg ggtcgctaag gcccgggcgg tgcagtagct tttccatcag
      721 gcggaccgcc agttgctgtt cgttctggct gtggtcgtaa taggtctgca gcagcgccgc
      781 gttttcctgc aattggcggg cggccttatg gcgctccgaa atgtcgcgga tgatgcagac
      841 gtagagcccc tcgtggtcga tcgaaacttc ggagatgccg atctcgaccg gaaagtcggc
      901 gtgatcgccg cgccgaccga cggtttcccg gtcccttccc ttgtaggcgc tgagcgcgca
      961 gatcggtcgg ccatggcggt cggtagcggt gttgccggaa ggcagttccg gtaacaaggt
     1021 actgaccggt tgccccagca ggtcgcggat gcgacggccg aaaatgcgtt cggttgcgcg
     1081 attcagactc aggatctcgc ccttgcaatc gaccgtgatg atggcgtcga ggacgttgtc
     1141 ggagatcgcc tgcagacgct cgagcgatac cgcggcactc tgctgcaggg ccagcgagcg
     1201 ctgcatcgag cgcaatttgg cttccagaac gacgaagttg atcggttttg tcaggtagtc
     1261 gtcggcgccg gcctcgaggc cttcgaccag gttctcgtcg cggttgagcg ccgacaggaa
     1321 aatgatcggc acccacttgt ccttgcttgc cgccttgatc cggcgggccg cctcgaaacc
     1381 atccatgacc ggcatcatga tgtcgagcag aatcagatcc gggcggtcct tcagaaagcg
     1441 ctgcaccgcc tgttcgccgt tctcggcgag gatgacgtca tgcccgagct tcttcaggaa
     1501 gacctggaga atatgcagat tcgtgcgatt gtcgtcgacc gccagaacgc tcatcttgtc
     1561 gaatgcgttg ctcatttttg ctggcctgta ctcaccgatg gaaagccgtt gttcgataat
     1621 ccgatcgaat catctatgac tggcggatgc cggctagg
//
LOCUS       DAOSDX010000716         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24563_length_1657_cov_3.480025, whole genome shotgun sequence.
ACCESSION   DAOSDX010000716 DAOSDX010000000
VERSION     DAOSDX010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24563_length_1657_cov_3.480025"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLN02_13515"
     CDS             complement(<1..350)
                     /locus_tag="PLN02_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436651.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI53392.1"
                     /translation="MNYQWNWHVFLQPTAAGDDIYLGWMLSGLQMTLMLSLSAWLIAL
                     LLGAVMGVLRTVPNKALSGLATAYVELFRNIPLLVQLFIWYFVLPELLPTSIGDAYKQ
                     SHPVFQQFLAAMVCL"
     gene            complement(409..1473)
                     /locus_tag="PLN02_13520"
     CDS             complement(409..1473)
                     /locus_tag="PLN02_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOI53393.1"
                     /translation="MFKRIFTRCAVFAIGCAASLAVMAAQPNVVILATGGTIAGAGAE
                     ATNSATYQAAKVPVDKLIAGVPELAKVAAVRGEQVFQIASESFTNDHLLTLGKRVSAL
                     AKQADVDGIVITHGTDTLEETAYFLNLVVHTDKPIVIVGSMRPGTAMSADGMLNLYNA
                     VAAAAAKDARGKGVLVAMNDELNSGRDVSKMINIQTSAFKSPWGPLGMIVEGRNYWFR
                     LPAKRHTVNSEFDIDKIDSLPLVGIAYGHGNVADTAYKALAASGAKAIIHAGTGNGSV
                     AKNVVPALRELRGQGVQVIRSSHVNAGGFVLRNAEQPDDQYDWVVAHDLNPQKARILA
                     SVALTKTNDSKELQRIFWEY"
BASE COUNT          280 a          484 c          579 g          314 t
ORIGIN      
        1 aggcagacca tcgccgccag gaactgctgg aacaccgggt gcgactgctt gtaggcgtca
       61 ccgatgctgg tcggcaacag ttccggcagc acgaagtacc agatgaacag ttgcacgagc
      121 agcgggatgt tgcggaacag ctcgacgtag gcggtggcca ggccgctcag cgccttgttc
      181 ggcaccgtgc gcaggacacc catgacggcg ccgagcagga gggcgatgag ccaggcgctc
      241 agcgagagca tcagggtcat ctgcaggccg gacagcatcc agcccaggta aatgtcgtcg
      301 cccgccgccg tcggctgcag gaagacgtgc cagttccatt ggtagttcat gacttttcct
      361 cgtcgaaaca gggaaaccgg gcgcgccccg cgcgcccggc ggacgggatc agtattccca
      421 gaagatgcgc tgcagttcct tgctgtcgtt ggtcttggtc agagcgacgc tggccaggat
      481 gcgggccttc tgcgggttga ggtcgtgagc gacgacccag tcgtactggt cgtccggctg
      541 ttcggcgttg cgcagcacga agccgccggc attgacatgc gacgaacgga tgacctggac
      601 gccctggccg cgcagttcgc gcagcgccgg aacgacgttc ttggccaccg agccgttgcc
      661 ggtaccggca tggatgatcg ccttggcacc gctggcggcg agcgccttgt aggcggtatc
      721 ggcgacgttg ccgtgaccgt aggcgatgcc gaccagcggc aggctgtcga tcttgtcgat
      781 gtcgaactcg gagttcacgg tatggcgctt ggccggcagg cggaaccagt agttcctgcc
      841 ttcgacgatc atgccgagcg gaccccaggg gctcttgaag gcgctggtct ggatgttgat
      901 catcttggac acgtcgcggc cgctgttcag ttcgtcgttc atcgccacga gcacgccctt
      961 gccgcgggcg tccttggcgg cagcggcggc gacggcgttg tacaggttga gcatgccgtc
     1021 ggccgacatc gcggtacccg gacgcatcga accgacgatg acgatcggct tgtcggtatg
     1081 gacgacgagg ttgaggaagt aggcggtctc ttccagcgtg tcggtaccgt gggtgatgac
     1141 gatgccgtcg acgtcggcct gcttggccag cgccgaaaca cgcttgccga gggtcagcag
     1201 gtggtcgttg gtgaagcttt cggaagcgat ctggaacacc tgctcgccac gcacggcggc
     1261 aaccttggcg agttccggga cgccggcgat cagcttgtcg accggcactt tggcggcctg
     1321 gtaggtggca ctgttggtgg cttcggcgcc ggcacccgca atggtgccgc cggtggcgag
     1381 gatgacgacg ttgggctggg cggccatgac cgccagcgag gcggcacagc cgatcgcgaa
     1441 gacggcgcaa cgggtgaaaa tacgcttgaa catgatgaac tcctcttttt ttggggatga
     1501 gccgggcctg gcaccccgtt ccgggagtgc ctcgatcacg gcggatttct cgggaaatta
     1561 ttcgaaagct ttgtcgttcg gtgccttgta cagggccttc atttcagccg acagcggcat
     1621 gttgaggttc agccccttgg gcgggatcgg gttcatg
//
LOCUS       DAOSDX010000717         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24588_length_1656_cov_3.232979, whole genome shotgun sequence.
ACCESSION   DAOSDX010000717 DAOSDX010000000
VERSION     DAOSDX010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24588_length_1656_cov_3.232979"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLN02_13525"
     CDS             <1..911
                     /locus_tag="PLN02_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOI53394.1"
                     /translation="PFVDERLMKRIEQMNAAAFLVHPYRRPIIGWMDDLENMSATSLK
                     AWYDAWYVPNNAYVVVSGDVDHQAVFELARQHYGGLAARPLPARRDLVEPRQEGPRRI
                     TVKAPAELPSLILAYKAPILRDPKQDRDPYALEILTAILNGHDAARFNKKLVREDKVA
                     LSAGVGYDSTARGPGLIFLYGTPSEGRTIAELEAALRAEIDRIRQEGVSEAELKRTRA
                     QLIAGQVYKLDSMFGQAMEIGQTEAVGLSWKDVETMRERLQQVSAADVQAVAKKYFDD
                     DGLTVGILDPQALDGKPRRPAVATRH"
     gene            923..>1656
                     /locus_tag="PLN02_13530"
     CDS             923..>1656
                     /locus_tag="PLN02_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOI53395.1"
                     /translation="MKRLLTAAFTLIVAAAAQAGVTIEQWTTGNGTRVNFVAAPTLPM
                     LDVQVDFAAGSMFDPAGKSGLAALTRGVLDLGAGARDETAIAEQLADLGAQLSGSADT
                     DRASVTLRTLTAKDKLDPALAVLRDVLHQPHFDPAIFEREKARTIAGLKDAMTRPDSI
                     AGKAFWEALYPGHPYGRQASPESVATLSRDDVVAFHGRFYNAANAQISLVGQLTRQQA
                     EAIAEQLSAALPAGTPAELPPAPAAAR"
BASE COUNT          289 a          633 c          526 g          208 t
ORIGIN      
        1 gtccgttcgt cgacgagcgc ctcatgaagc gcatcgagca gatgaacgcc gccgccttcc
       61 tcgtccatcc ctaccggcgg ccgatcatcg gctggatgga cgacctcgag aacatgagcg
      121 cgaccagcct gaaggcctgg tacgacgcct ggtacgtgcc gaacaatgcc tacgtcgtgg
      181 tcagcggcga cgtcgaccat caggcggtgt tcgaactcgc ccgccagcat tacggcgggc
      241 tcgcggcacg cccgctgccc gcccgccgcg acctggtcga accgcgccag gaggggccgc
      301 gccggatcac ggtcaaggcg ccggccgaac tgccgtcgct gatcctcgcc tacaaggcgc
      361 cgatcctgcg cgacccgaag caggaccgcg acccctacgc gctggaaatc ctcaccgcca
      421 tcctcaacgg ccacgacgcc gcccgcttca acaagaagct ggtgcgcgaa gacaaggtcg
      481 cgctgtcggc cggcgtcggc tacgacagca cggcacgcgg cccggggctg atcttcctct
      541 acggcacgcc gtcggaaggg cgcacgatcg ccgagctgga agccgcgctg cgcgccgaga
      601 tcgaccgcat ccgccaggag ggcgtcagcg aggccgaact gaagcggacg cgcgcccagt
      661 tgatcgccgg ccaggtctac aagctcgatt cgatgttcgg ccaggccatg gaaatcggcc
      721 agaccgaggc ggtcggcctg tcctggaagg acgtcgagac gatgcgcgaa cgcctgcagc
      781 aggtcagcgc cgccgacgtg caggcggtgg cgaagaagta tttcgacgac gacggcctga
      841 ccgtcggcat cctcgacccg caagccctgg acggcaagcc gcgccgtccg gccgtcgcca
      901 cccgtcactg atgaaacccg ccatgaaacg actgctcacc gccgccttca ccctgatcgt
      961 tgccgccgcc gcgcaggccg gcgtgaccat cgagcaatgg accaccggca acggcacccg
     1021 ggtcaatttc gtcgccgcgc cgaccctgcc gatgctcgac gtgcaggtcg attttgccgc
     1081 cggctcgatg ttcgacccgg caggcaaatc cggcctggcc gcgctgaccc gcggcgtact
     1141 cgacctcggc gccggcgccc gcgacgaaac ggcgatcgcc gaacagctcg ccgacctcgg
     1201 cgcccagctc agcggcagcg ccgacaccga ccgcgccagc gtcaccctgc gcacgctgac
     1261 cgccaaggac aagctcgatc cggcgctggc cgtgctgcgc gacgtactgc accagccgca
     1321 tttcgacccg gcaatcttcg agcgcgagaa ggcgcgcacc atcgccggcc tcaaggacgc
     1381 catgacacgc cccgacagca tcgccggcaa ggccttctgg gaggcgctct accccggcca
     1441 cccctacggc cgccaggcgt cgccggaaag cgtcgcgacg ctcagccgcg acgatgtcgt
     1501 cgctttccac ggccgcttct acaacgccgc caacgcgcag atctcgctgg tcggccagct
     1561 gacccggcag caggcggaag cgattgccga gcaattgagc gccgccctgc ccgccggcac
     1621 gccggccgaa ctgccgccag cgccggcagc ggcccg
//
LOCUS       DAOSDX010000718         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24615_length_1655_cov_2.529375, whole genome shotgun sequence.
ACCESSION   DAOSDX010000718 DAOSDX010000000
VERSION     DAOSDX010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24615_length_1655_cov_2.529375"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1505
                     /locus_tag="PLN02_13535"
     CDS             297..1505
                     /locus_tag="PLN02_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOI53396.1"
                     /translation="MALFLSAPAAAGPLPPILIAHVAPTTGRFALHAEADRRGAEMAI
                     EDFNRQGGVLGREIVLISSNPTMDAKKSAEVAETLITRNKVGFMVGAIASNVAASMSA
                     VAQKYGVIFINTNSSAPSESLENAHRSKFVFDASGANFNRALLKYALKDRKSKRVLQL
                     IEDNAAGHSNAEAMRPYIAEYGGQLVGEVLAPENLSDPTEVLNQVAAIPADVVTVVIS
                     GDNQIKLFSRIDPRVLERQFWIVGEVDWEELYPAPGTPRPLFGTTWAWNLDTPGSQAF
                     VERYRQRYGHTRLDWPGDVTHAAYLATRALLEAIARAGTTDNHAIIAQLEQYRAEPRS
                     RMQHDAAYMDPVSHHLQQTIYIARWKPDPARPERGIEIVGRVSPEQAQHAPEKNTRLE
                     SLAETPRYSP"
BASE COUNT          311 a          552 c          519 g          273 t
ORIGIN      
        1 gccctgagcg ctcggccggg gatctgccgg tcaggtagct gcgccgcgcc ggccatcgaa
       61 cgatgcctgg cgctcctgga aggagtgatg ccaaatgcat ggcgattggc cgtcttcccc
      121 gcttgcggca gcctccggac ggctggccgc cggccgttcc tgctcttcca gcgcagtttt
      181 ccatcggtcc ggccggtcgc tccgtggcga gatagcggag tatgattcgg gaaaacaagc
      241 tggaatcagg gagcgaatcg caaatgcgga taaaaaagcc atttggcatt ctggcgttgg
      301 cgctgttcct gagcgccccc gccgcagccg ggccgttacc gccgatcctg attgcccatg
      361 tcgcaccgac caccggccgc tttgccctgc atgccgaagc ggaccggcgc ggcgccgaga
      421 tggcgatcga ggatttcaac cggcagggcg gcgtgctggg tcgggaaatc gtcctgattt
      481 cgagcaatcc gacgatggac gccaaaaagt ccgccgaggt ggcggagacg ctgatcaccc
      541 gcaacaaggt cggcttcatg gtcggcgcca tcgcctccaa cgtcgcggca agcatgtcgg
      601 cggtggcgca gaaatacggc gtgatcttca tcaacaccaa ttcctcggcg ccttccgaat
      661 ccctggaaaa cgcccaccgc agcaagttcg tgttcgatgc cagcggcgcc aatttcaacc
      721 gggcgctgct caagtacgcg ctcaaggacc gcaaatcgaa acgcgtcctg caactgatcg
      781 aggataacgc ggccggtcac agcaatgccg aggcgatgcg cccctatatc gccgaatacg
      841 gcgggcagct ggtcggcgag gtgctggcgc cggaaaacct gagtgatccg accgaagtgc
      901 tgaaccaggt ggcggcgatt ccggccgatg tggtcaccgt ggtgatcagc ggcgacaacc
      961 agatcaagct gttttcccgg atcgacccca gagtgctcga acggcagttc tggatcgtcg
     1021 gcgaagtgga ctgggaagag ctctacccgg cgccggggac gccgcgtccg ctgttcggca
     1081 cgacctgggc ctggaatctc gatacgccgg gcagccaggc cttcgtcgag cgctaccgcc
     1141 agcgctacgg tcacacccgg ctcgactggc cgggtgacgt cacgcacgcc gcctatctgg
     1201 ccacccgcgc cctgctcgaa gccatcgcca gggccggcac caccgacaac cacgccatca
     1261 tcgcgcaact ggagcaatac cgggccgagc cgcgctcgcg catgcagcac gacgccgcct
     1321 acatggaccc ggtcagccat cacctgcagc agaccatcta catcgcgcgc tggaagcccg
     1381 atccggcgcg ccccgagcgc ggcatcgaga tcgtcggccg cgtcagtccg gaacaggcgc
     1441 agcatgcgcc ggaaaaaaac acccggctgg aatccctggc cgaaacaccg cgctattcgc
     1501 cctgaaccat gaccgggaaa tttggcctga tcggccgcat cgccttgctg gtagtgctcg
     1561 ttgaaatcgt tgtcttcggt gttctcggcg ggttctacgt cagccgtttc agcagcgccc
     1621 tcgacgagag gatgatcgcc ggtctgcgca acgtc
//
LOCUS       DAOSDX010000719         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24625_length_1654_cov_3.457786, whole genome shotgun sequence.
ACCESSION   DAOSDX010000719 DAOSDX010000000
VERSION     DAOSDX010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24625_length_1654_cov_3.457786"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLN02_13540"
     CDS             complement(<1..231)
                     /locus_tag="PLN02_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI53397.1"
                     /translation="MTRRYRFQALDANQQLVHGQMNATDPDELARLLRQHDLDLLLAR
                     RIPALNQRKLARRELIDFCFHLEQLLTAGISLL"
     gene            complement(231..>1654)
                     /locus_tag="PLN02_13545"
     CDS             complement(231..>1654)
                     /locus_tag="PLN02_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOI53398.1"
                     /translation="RYDPGRHTISREAILLVPSETARQHRLLPIAIEREQRRWRLALA
                     SPQDPGALESVRRHTPAGLSAEILLASEGEIESALDLHYGHALAIENLLGEFEGSGKI
                     ASLHGDSPAVRLIDALLGDAVRRHASDLHFAPEQACLRIRYRIDGILRTIRILHKSTW
                     PPLASRLKILAGMNIAETRAAQDGHFCRPIGGREIDFRVAAQPTIHGENIVLRILDRQ
                     KGIVPLESLGIGKNELQRIETMLARPEGLLLVTGPTGSGKTTTLYSLINRLNHDGVNI
                     MTLEDPVEYPLPLLRQTSLSDNIKLDFAGGIRSMLRQDPDIVLIGEIRDAETAAMALR
                     ATLTGHRVFATLHAGSAISALSRLRELGIDPRQLHGNLSGIIAQRLLRRRCPSCTTAT
                     PERDCPRCGGSGYNGRLALLEILRIDAGLDLLLADAASPAQLLAEARRRGFASLAENG
                     LQLVDAGVTTRAEVARVVDLSEY"
BASE COUNT          262 a          467 c          619 g          306 t
ORIGIN      
        1 gagcagtgaa atgcccgccg tcagtagctg ttcgagatgg aagcagaaat cgatcagttc
       61 ccggcgcgcc agcttgcgct ggttcaaggc tggaatccgg cgggccagga gcaggtccag
      121 atcatgctgg cgcaacagcc gcgccagttc gtccgggtct gtggcgttca tctgcccgtg
      181 taccagttgc tggttggcat ccagtgcctg gaagcggtag cggcgcgtca ttaatattcg
      241 ctcaggtcga cgacccgagc cacttcggcg cgcgtcgtca caccggcatc gaccagttgc
      301 agtccgttct cggccaggct ggcaaagccg cggcgccggg cttcggccag cagttgcgcc
      361 ggactggcag cgtcggcgag caacaggtcg agtccggcat cgatgcggag aatttccagg
      421 agggcgagac ggccgttgta gccgctgccg ccgcagcgcg ggcaatcccg ctccggtgtg
      481 gctgtcgtgc aggacgggca gcggcgacga agcaggcgct gggcaatgat gccggaaagg
      541 ttgccgtgca actggcgggg gtcgatgccg agttcgcgca ggcgggagag tgctgatatt
      601 gcactaccgg cgtgcagggt ggcgaagaca cggtggccgg tcagggtggc gcgtagcgcc
      661 atggcggcgg tttcggcatc gcggatttcg ccgatcagga cgatgtccgg gtcctggcgg
      721 agcatggagc ggatgccgcc ggcaaaatcc agcttgatgt tgtcggagag tgaggtctgg
      781 cgcagcaggg gcaaagggta ttcgaccggg tcctcgagcg tcatgatatt gacgccatcg
      841 tggttgagcc ggttgatcag cgagtaaagc gtggtcgtct tgccgctgcc ggtcgggccg
      901 gtgaccagaa gcagtccttc cgggcgggcc agcatggttt cgatacgttg cagttcgttc
      961 ttgccgatgc cgagggattc gagcgggacg atgccttttt gccggtcgag gatgcgcagg
     1021 acgatgtttt ccccgtggat ggtcggttgg gcggcaaccc ggaagtcgat ctcccgaccg
     1081 ccgatcggcc ggcagaagtg tccgtcctgt gccgcgcggg tttccgcgat gttcatgccg
     1141 gcaaggattt tcaggcggct ggccagcggc ggccaggtcg atttgtgcag gatgcggatc
     1201 gtccgcagga tgccatcgat gcggtagcgt atacgcaggc aggcctgctc gggggcgaag
     1261 tggaggtccg aggcatgacg gcggacggcg tcgccgagca gtgcgtcgat cagccggact
     1321 gccggcgagt ctccgtgcag cgaagcgatc ttgccgctgc cttcgaattc gccgaggagg
     1381 ttttcgatgg ccagcgcatg gccgtaatgc aggtcgaggg cgctttcgat ttcgccctcg
     1441 ctggcgagca ggatttccgc cgagaggccg gcgggcgtgt ggcggcgcac gctttcgagc
     1501 gcgccggggt cttgcggcga ggccagcgcc aggcgccagc gtcgctgctc ccgctcaatg
     1561 gcgatcggca gcaggcggtg ctgcctggcg gtctcggaag ggacgagaag aatcgcttcg
     1621 cggctgatcg tatgtcggcc cgggtcgtaa cgtt
//
LOCUS       DAOSDX010000720         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24630_length_1654_cov_3.101939, whole genome shotgun sequence.
ACCESSION   DAOSDX010000720 DAOSDX010000000
VERSION     DAOSDX010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24630_length_1654_cov_3.101939"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..897
                     /locus_tag="PLN02_13550"
     CDS             121..897
                     /locus_tag="PLN02_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOI53399.1"
                     /translation="MDYLYTLSGFAVGAIVGLTGVGGGSLMTPLLVLLFGVHPATAVG
                     TDLLYAAMTKAGGTVAHGRKGHIDWQVTGRLAAGSIPAAALTIWVLSLLPDGSNAISK
                     IVSHGLGFALLLTAIAILFGRKLREYASRHDESPLRQCCLGKITVGVGVVLGVLVTIS
                     SVGAGALGVAALFFLYPKLSPVKIVGSDVAHAVPLTLVAGLGHWLLGSVDWALLGALL
                     LGSLPGIWLGSHLSAKVPEHILRRILASMLVLIGSKLVFA"
     gene            918..>1654
                     /locus_tag="PLN02_13555"
     CDS             918..>1654
                     /locus_tag="PLN02_13555"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOI53400.1"
                     /translation="MYKYDAIDRQLILDRVEQFRDQVRRWQAGELTDDEFRPLRLQNG
                     LYIQRHAPMFRIAVPYGMLNSAQLRKLAHVARQYDRGYGHFTTRHNLQLNWPKLTDVP
                     DILAELAEVDLHAIQTSGNCLRNITTDQFAGVAADEIIDPRPLAEILRQWTTFHPEFA
                     FLPRKFKIAISGTEEDRAVTWFHDIGLHLKVIDGVTGFTVIVGGGLGRTPIRGQVVRD
                     FLPWQHILSYCEAILRVYNRFGRRDNA"
BASE COUNT          265 a          613 c          475 g          301 t
ORIGIN      
        1 tcgatctcga cgatgtagcg cagttgctga agcttcatga atggcttaat aacaaaaaac
       61 gataacaatc taacccaata ttctttttga ttcaatcaac gcccggtagg atgccgaccc
      121 atggactacc tgtacaccct ttccggcttc gcggtcggcg ccatcgtcgg cctgacgggc
      181 gtcggcggcg gttcgctgat gactccgctg ctggtgctgc tgttcggcgt gcatccggcg
      241 acggctgtcg gcaccgacct gctctacgcg gcgatgacca aggccggcgg caccgtcgcc
      301 cacggccgca agggccatat cgactggcag gtcaccggcc gcctcgccgc cggcagcatc
      361 ccggcggcgg cgctgacgat ctgggtgttg tcgctgctcc ccgatggcag caacgcgatc
      421 agcaagatcg tctcgcacgg cctcggcttc gccctgctgc tcaccgccat cgccatcctg
      481 ttcggccgca agctgcgcga atacgccagc cgccacgacg aatcgccgct gcgccagtgc
      541 tgcctaggca agatcacggt cggcgtcggc gtcgtgctcg gcgtgctggt gacgatatcc
      601 tcggtcggcg ccggcgcgct cggcgtcgct gccctcttct tcctttaccc gaagttgtcg
      661 ccggtcaaga tcgtcggctc cgacgtcgcc cacgccgtgc cgctgacgct ggtcgctggc
      721 ctcggccact ggctgctcgg cagcgtcgac tgggccctgc tcggcgccct gctgctcggc
      781 tcgctgcccg gcatctggct cggcagccat ctctccgcca aggtcccgga gcacatcctg
      841 cgccgcatcc ttgcctccat gctcgtgctg atcggcagca agctggtctt cgcctgattc
      901 acccccaaga gaacgccatg tacaaatacg acgccatcga ccgccagctg atcctcgacc
      961 gggtcgagca gttccgtgac caggtccgcc gctggcaggc cggcgagctg accgacgacg
     1021 agttccgccc gctgcgcctg cagaacggcc tctacatcca gcgccacgcg ccgatgttcc
     1081 gcatcgccgt gccctacggc atgctcaatt cggcgcaact gcgcaagctg gcccacgtcg
     1141 cccgccagta cgaccgcggc tacggccatt tcaccacccg ccacaacctg cagttgaact
     1201 ggccgaagct gaccgatgtg cccgacatcc tggccgaact ggccgaggtc gacctgcacg
     1261 cgatccagac ctcgggcaac tgcctgcgca acatcaccac cgaccagttc gctggcgtcg
     1321 ccgccgacga gatcatcgat ccgcgtccgc tggccgaaat cctccgccag tggaccacct
     1381 tccacccgga attcgccttc ctgccgcgca agttcaagat cgccatttcc ggcaccgagg
     1441 aagaccgcgc ggtcacctgg ttccacgaca tcggcctgca tctcaaggtg attgatggtg
     1501 tcaccggttt caccgtgatc gtcggcggcg gcctcggtcg cacgccgatc cgcggccagg
     1561 tcgtgcgcga cttcctgccc tggcagcaca tcctcagcta ctgcgaggcg atcctgcgcg
     1621 tctataaccg cttcggccgc cgcgacaacg ccta
//
LOCUS       DAOSDX010000721         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24728_length_1649_cov_3.918444, whole genome shotgun sequence.
ACCESSION   DAOSDX010000721 DAOSDX010000000
VERSION     DAOSDX010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24728_length_1649_cov_3.918444"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..695
                     /gene="queC"
                     /locus_tag="PLN02_13560"
     CDS             18..695
                     /gene="queC"
                     /locus_tag="PLN02_13560"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOI53401.1"
                     /translation="MNEKAVVLLSGGLDSATCLAIARNQGFDCYCLSFNYGQRHSAEL
                     QAADRVVKALGAAEHRVINFGLAQFGGSALTDTSIAVPTGGVQPGIPVTYVPARNTIM
                     LSLALAWAEVLGSRDIFVGVNAVDYSGYPDCRPEYIAAFEKMANLATRAGVEGQKLHI
                     HAPLIDLSKAEIIRTGHALGVDYGLTVSCYQADELGRACAVCDSCRLRAEGFAAAGLP
                     DPTPYRT"
     gene            697..>1649
                     /locus_tag="PLN02_13565"
     CDS             697..>1649
                     /locus_tag="PLN02_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOI53402.1"
                     /translation="MDVDVSPNTVLWLTFAIAFVFGAIGQKTHFCTMGAVSDILNMND
                     WSRMRMWLLAIAVAILGAGALDAGGLIDLDKSIYRTTSLTWLSYLVGGFSFGVGMVLA
                     SGCGSKTLIRIGSGNLKSLVVFLVLGLFAYMTMRGVFGVFRVRFLDPVAVAMPEGQDI
                     PSLLAGLGLAETSAATLAVLAIGGGLLAFCLLRRDFWTLDNLLGGLGTGLLVVGAWYV
                     SGHLGYLPEHPDTLEEAFIATNSGRMESLTFVAPVAYTLELFMLWSDSSRVLTVAIVS
                     VFGVIAGSAAWSLATRSFRWEGFASVEDTASHLIGAALMGFG"
BASE COUNT          238 a          615 c          490 g          306 t
ORIGIN      
        1 gggtatattc gcgccccata aacgaaaaag ccgtagtcct tctatccggc ggtctggact
       61 ccgccacctg cctcgccatt gcgcgcaacc agggtttcga ctgctactgc ctgtccttca
      121 actacgggca gcggcacagc gccgaactgc aggccgccga ccgtgtggtc aaggcgctcg
      181 gcgccgccga acaccgggtg atcaatttcg gcctcgccca gttcggcggc tcggcgctca
      241 ccgacacctc gatcgccgta ccgaccggcg gcgtgcaacc gggcatcccg gtcacctacg
      301 tgccggcgcg caacaccatc atgctctcgc tggccctggc ctgggccgaa gtgctgggca
      361 gccgcgacat cttcgtcggc gtgaacgcgg tcgactactc cggctacccg gattgccgcc
      421 cggaatacat cgccgctttc gagaaaatgg ccaacctggc gacccgggcc ggcgtcgaag
      481 ggcagaagct gcacatccac gcgccgctga tcgacctttc caaagccgaa atcatccgca
      541 ccggccatgc gctgggcgtc gactacgggc tcaccgtttc ctgctaccag gccgatgaac
      601 tcggccgcgc ctgcgctgtc tgtgactcct gccggctgcg cgccgagggc ttcgccgccg
      661 ccggcctccc cgatcccacg ccttaccgga cctgacatgg acgtcgacgt ttccccgaac
      721 accgtactct ggctgacttt cgccatcgcc ttcgtcttcg gggcgatcgg ccagaaaacc
      781 catttctgca ccatgggcgc ggtttccgac atcctcaaca tgaatgactg gagccgcatg
      841 cgcatgtggc tgctcgccat tgccgtcgcc atcctcggcg ccggcgcgct tgacgccggc
      901 ggcctgatcg acctcgacaa gtcgatctac cgtactacca gcctgacctg gctgtcctac
      961 ctggtcggcg gcttcagctt cggcgtcggc atggtgctgg cttccggctg cggctcgaag
     1021 acgctgatcc gcattggcag cggcaacctg aaatcgctgg tcgtcttcct cgtcctcggc
     1081 ctcttcgcct acatgacgat gcgcggcgtc ttcggcgtct tccgcgtcag gttcctcgac
     1141 ccggtcgccg tggccatgcc ggaagggcag gacattccgt cgctgctcgc cggcctcggc
     1201 ctcgccgaaa cgagcgccgc gaccctggcc gtcctcgcca tcggcggcgg cctgctcgcc
     1261 ttctgcctgc tgcgccgcga cttctggacc ctggacaacc tgctcggcgg tctcggcacc
     1321 ggcctgctcg tcgtcggcgc ctggtatgtc agcggccacc tcggctatct cccggaacac
     1381 ccggacacgc tggaagaggc tttcatcgcc accaacagcg gccgcatgga atcgctgacc
     1441 ttcgtcgcgc cggtcgccta cacgctggaa ctgttcatgc tgtggtcgga cagcagccgc
     1501 gtcctgaccg tcgccatcgt ctccgtcttc ggcgtcatcg ccggctcggc ggcgtggtcg
     1561 ctggccaccc gcagcttccg ctgggaaggc ttcgccagcg tcgaggacac cgccagccac
     1621 ctgatcggcg ccgcgctgat gggcttcgg
//
LOCUS       DAOSDX010000722         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24744_length_1649_cov_1.427227, whole genome shotgun sequence.
ACCESSION   DAOSDX010000722 DAOSDX010000000
VERSION     DAOSDX010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24744_length_1649_cov_1.427227"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLN02_13570"
     CDS             <1..588
                     /locus_tag="PLN02_13570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOI53403.1"
                     /translation="FMFDISERKKNEQELLSLQKKLEELSYKDGLTGIANRRMFDSTL
                     ETEWADARRNQSALSLVMLDIDYFKQYNDAYGHIQGDICLTRVAGILSGAASRPRDFV
                     ARIGGEEFAIILPETDEKSARKVAERCRQALEKARIPHEGSSVSDFLTLSIGVATRIP
                     SALDQDVSLIEAADKSLYQSKNTGRDRISSPEAIS"
     gene            complement(601..1461)
                     /locus_tag="PLN02_13575"
     CDS             complement(601..1461)
                     /locus_tag="PLN02_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOI53404.1"
                     /translation="MQVVDEYRCHADFSTICRDHPPEIAWRQIIDVTSAALRQMLQRH
                     PDVASVGIGFPVFIDPVHGTVLQSPNLRGLRDVDLAGDLARALERPVVVENDANAAAY
                     GEYKLHPQAVPSLVYLGLGTGVGGGLVIRHKLITGSHGFAMEAGHLIVEAGGRLCGCG
                     NRGCMEQYASASGVSLSYAEKSGKRLNASEVAALAQAGDDAARAAFERAGDALAQALA
                     HILKVVDVENIVIGGGMSKAWPLMQPAFQARLEMDLIPVLRGRVDIRLSDSGDHAGVI
                     GAAALSAGVV"
     gene            1402..1578
                     /locus_tag="PLN02_13580"
     CDS             1402..1578
                     /locus_tag="PLN02_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53405.1"
                     /translation="MIAADGGEIGMAAVFVDDLQVGDQRNAQVGAADIDADTFLLVSP
                     SAPPPLSLRLLSPP"
BASE COUNT          326 a          551 c          490 g          282 t
ORIGIN      
        1 ttcatgttcg acatcagcga gcgcaagaag aacgagcagg aattgctgtc gttgcagaag
       61 aaactggagg agctctccta caaggacggt ctgaccggca tcgccaaccg ccgcatgttc
      121 gattccaccc tggagaccga atgggccgac gccaggcgca accagtcagc gctttcgctg
      181 gtcatgctcg acatcgacta tttcaagcaa tacaacgacg cctatggtca catccagggc
      241 gatatctgcc tgacacgggt cgccggtatc ctcagtgggg ccgcatcgcg tccgcgcgat
      301 tttgtcgccc gcatcggcgg cgaggagttt gccatcatcc tgccggaaac cgatgagaaa
      361 tccgccagga aagtggcgga acgctgccgc caagccctgg aaaaggcaag gattccgcac
      421 gaaggatcca gtgtcagcga tttcctcacc ctcagcatcg gcgtcgccac acggattccg
      481 tcagcgctcg accaggatgt cagcctgatc gaggcagccg acaaatcgct gtatcagtcg
      541 aagaacactg gccgcgaccg catcagcagc ccggaggcaa taagctaaga gcgttcccgc
      601 tcagaccact ccggcagaaa gcgcggccgc accgatgacg ccggcatggt cgccgctgtc
      661 cgaaaggcgg atatccaccc tgccgcgcag cacaggaatc agatccattt ccagccgcgc
      721 ctggaacgcc ggttgcatca acggccaggc cttgctcatg ccgccgccga tgacgatgtt
      781 ttccacatcg accaccttga ggatatgcgc cagcgcttgt gccagcgcgt cgccggcccg
      841 ctcgaacgcg gcccgggcgg catcgtcgcc cgcttgcgcc agcgcggcga cttcactggc
      901 attcagacgt tttccgctct tctccgcgta actgaggctg acgccgctgg ccgaggcata
      961 ctgctccatg cagccgcggt tgccgcagcc gcacaggcgc ccgcccgcct cgacgatcag
     1021 atggcctgcc tccatggcga agccatggct gcccgtgatc aatttgtgac gtatgaccag
     1081 tccaccgccg acgccggtcc cgaggccgag atagaccagg ctggggaccg cttgcggatg
     1141 caacttgtat tcgccgtagg ctgccgcatt ggcgtcattc tcgaccacga ccgggcgctc
     1201 cagcgcgcgg gccagatcgc cggccagatc gacatcgcgc agaccccgca gattgggcga
     1261 ttgcaacacg gtgccatgca ccgggtcgat gaaaacggga aagccgatgc cgaccgatgc
     1321 cacatccggg tgccgttgca gcatttgccg cagcgcggca gaagtgacgt cgatgatctg
     1381 ccgccaggcg atctccggcg gatgatcgcg gcagatggtg gagaaatcgg catggcagcg
     1441 gtattcgtcg acgacttgca ggtcggcgac cagcgcaacg cgcaggttgg tgccgccgac
     1501 atcgacgccg atacgtttct gcttgtcagt ccttccgccc cgccgccgct cagtctgcgg
     1561 ctactatccc cgccgtgacg cggatggcga tttccagttc atccgtcaac gcatcggatg
     1621 gccattccag caacagagtc accgcctgc
//
LOCUS       DAOSDX010000723         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24802_length_1646_cov_2.530484, whole genome shotgun sequence.
ACCESSION   DAOSDX010000723 DAOSDX010000000
VERSION     DAOSDX010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24802_length_1646_cov_2.530484"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLN02_13585"
     CDS             complement(<1..784)
                     /locus_tag="PLN02_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI53406.1"
                     /translation="MILSLPLRWCTDGERLPGRLLLAAAVALALFLVMPLLALLIGSV
                     RDDEGAFSLLRFAEFAATPGMATAAWNTLWVAALVTALTVPLAFLYAYAIQRACLPLR
                     GLWRLIGLSALLGPSLVGAIAFIQWFGTQGVLKAWLGGASVYGPVGIVMATVYAAFPH
                     ALMILVVALATADGRLYEAADALGASRWRRFRTVTLPAAKYGLISAALVVFAYSVSEF
                     GIPKVIGGNFPVLAVEVYVQVVGQHNFGRGAIVALLLLLPVLF"
     gene            complement(781..>1646)
                     /locus_tag="PLN02_13590"
     CDS             complement(781..>1646)
                     /locus_tag="PLN02_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOI53407.1"
                     /translation="QLSPALRDYGIVFQSYALFPNLSVADNVAYGLQHGRSDRRLIQQ
                     RVEEMLELVGLPGTQAKYPAQLSGGQQQRIALARALATAPDLLLLDEPLSALDAIERI
                     RLRGEIRALQTRLGVTTIMVTHDQEEALAMADRIVVMQRGRIQQIGAPRDIYRQPGND
                     FVADFIGRGNLLVAERVGPHHARSGDLLLRTGAELPAAATARFYVRPEDIRLHDTLDQ
                     GENTFYGHVLKCEFLGPYSLVTLRAPTADGRPLVAQFSSNYLCGREIRPGALLRLGIP
                     AEHLQALPEAA"
BASE COUNT          296 a          537 c          601 g          212 t
ORIGIN      
        1 cgaacagcac cggcaacagg agcagcaggg cgacgatggc gccacgaccg aaattgtgct
       61 ggccgacaac ctggacatag acctcgaccg ccagcaccgg gaaattgccg ccgatgacct
      121 tggggatgcc gaactcgctg accgaatagg cgaacaccac cagcgccgcg ctgatcagcc
      181 cgtacttggc ggccggcagc gtcaccgtgc ggaaacgccg ccagcgcgag gcgccgagcg
      241 cgtcggcggc ttcgtagaga cgtccgtcgg cggtggccag ggcgacgacg aggatcatca
      301 gggcgtgcgg gaaagccgcg tagaccgtgg ccatgacgat gccgaccgga ccatacaccg
      361 aggcgccgcc cagccaggcc ttgagcacgc cctgggtgcc gaaccactgg atgaaggcaa
      421 tcgccccgac cagcgacggc ccgagcaatg ccgacaggcc gatcagccgc cacaggccgc
      481 gcagcggcag acaggcgcgc tggatggcgt aggcgtaaag gaaagccagc ggcaccgtga
      541 gcgccgtcac cagcgccgcc acccacagcg tgttccaggc cgccgtcgcc attccggggg
      601 tggcggcgaa ttcggcaaag cggagcaggc tgaaggcgcc ttcgtcgtcg cgcacgctgc
      661 cgatcagcag tgccagcagc ggcatcacca ggaacaaggc gagtgccacg gcggcggcga
      721 gcaacagacg accgggcaga cgctcgccgt cggtgcacca gcgcagcggc aggctgagga
      781 tcatgccgcc tccggcaagg cttgcaggtg ctcggccggg atccccaggc ggagcagcgc
      841 ccccgggcgg atttcgcggc cgcacaggta gttgctggaa aactgggcga ccagcggccg
      901 gccgtcggcg gtaggcgcgc gcagcgtgac cagcgaatac gggccgagaa actcgcactt
      961 gagcacatgg ccgtaaaacg tgttctcgcc ctggtcgagg gtatcgtgca ggcggatgtc
     1021 ttcggggcgg acgtagaagc gggcggtcgc tgcggccggc aactcggcgc cggtgcgcag
     1081 cagcaggtcg ccgctgcggg catggtgcgg cccgacgcgt tcggcaacga gcaggttgcc
     1141 gcggccgatg aaatcggcga cgaaatcgtt gccgggctgg cggtagatgt cgcgcggtgc
     1201 gccgatctgc tggatgcgcc cgcgctgcat gacgacgatg cggtcggcca tggccagggc
     1261 ctcttcctgg tcgtgcgtga ccatgatcgt ggtcaccccg agacgggtct gcagggcgcg
     1321 gatttcaccg cgcaggcgga tccgctcgat ggcgtcgagc gccgacagcg gttcgtcgag
     1381 cagcaacagg tcgggcgccg tcgccagcgc ccgggccagg gcaatgcgct gctgctggcc
     1441 accggacaac tgcgccggat acttggcctg ggttcccggc aggccgacca gttcgagcat
     1501 ttcctcgacc cgctgctgga tcaggcgacg gtcgctgcgt ccgtgctgca gtccgtaggc
     1561 gacattgtcg gcgacgctga ggttgggaaa cagcgcgtag gactggaaga cgatgccgta
     1621 gtcgcgcagc gccggcgaca gttgcg
//
LOCUS       DAOSDX010000724         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24868_length_1642_cov_4.198488, whole genome shotgun sequence.
ACCESSION   DAOSDX010000724 DAOSDX010000000
VERSION     DAOSDX010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24868_length_1642_cov_4.198488"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLN02_13595"
     CDS             complement(<1..406)
                     /locus_tag="PLN02_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53408.1"
                     /translation="MASKNVEIVVTNTVVAHYEARSYPDQQTDEAGYYPLYKVPVHRI
                     LVRGKNAAGAASSFEFMAPRFMPYFNDPEHPDPHYGVKGWANAGLSSANKIKVSRYKQ
                     DYAVHNRYSPGRGAIVLRSTFYIHAGPATLSDW"
     gene            complement(529..1077)
                     /locus_tag="PLN02_13600"
     CDS             complement(529..1077)
                     /locus_tag="PLN02_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53409.1"
                     /translation="MNLLAAYGLLAHGLIFGGLVSLLPLGVLRPHAALAGTTVALLAG
                     IAPALHALFGPPSLTLLLLALLHLQQRPLVPLTYPAALVIIGIAVLFYPAALGWGSFD
                     PYALGYQPLPLLAALAPLGLVLCWRGNNAWLLILAAGLAAYAGGLFANLWDALLDPLL
                     VLLALGVVLRGLAIRLIVARRR"
     gene            complement(1074..>1642)
                     /locus_tag="PLN02_13605"
     CDS             complement(1074..>1642)
                     /locus_tag="PLN02_13605"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOI53410.1"
                     /translation="PLAQAPLPLADGGFGLPLSQSGLRPHGAWLRVDQAGRIVDQRRL
                     PTGSAYPAIAATDTSSATALLAAFPGLAGARSDDGGQTWQTAPAPTVANPGTPLALLR
                     LTSGRLLLAGNEGTGRGRLALWLGDPQGREWHLARRVEDAADATADFSEPALLQAADG
                     RIHLVYAWRRQGIRHLVFNEAWLAGGQP"
BASE COUNT          265 a          490 c          643 g          244 t
ORIGIN      
        1 cccagtcgct cagcgtggct ggcccggcgt ggatgtagaa ggtgctgcgc aggacaatgg
       61 cgcctcggcc gggcgaatat cggttgtgaa ccgcgtaatc ctgcttgtag cgggaaacct
      121 tgatcttgtt ggccgacgac agtccggcgt tggcccatcc cttgaccccg taatgcgggt
      181 cgggatgctc tgggtcgttg aagtagggca tgaagcgggg agccatgaac tcgaagctac
      241 ttgcagcgcc ggccgcgttc ttgccgcgga ccaggatgcg atgcacgggt accttgtaga
      301 gcgggtaata gccagcctcg tcggtctgct ggtcggggta ggagcgcgct tcgtaatggg
      361 ctacgacggt gttcgttacg acgatttcca cgtttttcga tgccatcgat tttcctcgca
      421 aatggatgct gtcgaccaga ttggtgaata atgcagcggc ataattatat ggaatattcg
      481 tggcgatggc agcggctttc ctggaggggc ggatgttccg cctgtcgttc agcgtcgccg
      541 ggcgacgatc aggcggatcg ccagtccgcg caggacgaca ccgagcgcga gcaggaccag
      601 cagcgggtcg agcagggcat cccagaggtt ggcgaagaga ccgccggcgt aggcggcgag
      661 gccggcggcg aggatcagca gccaggcgtt gttgccgcgc cagcagagga caagaccgag
      721 cggcgccagc gctgccagca agggcaaggg ctggtagccg agggcgtagg gatcgaagct
      781 gccccagccg agcgcggccg ggtagaagag gacggcgatg cctatgatga ccagcgccgc
      841 cggataggtc agcggtacca gcggtcgctg ctgcaggtgg agcagcgcca ggagcagcag
      901 ggtcagcgag ggcggtccga acagcgcgtg cagtgccggc gcgatgccgg ccagcagggc
      961 gaccgtggtt cccgccagcg ccgcgtgcgg acgcagcacg ccgagcggca gcaggctgac
     1021 caggccgccg aagatcaggc cgtgggcgag caggccgtag gcggcgagca ggttcatggc
     1081 tggcctccgg cgagccaggc ctcgttgaag acgaggtggc ggatgccctg gcggcgccag
     1141 gcataaacga ggtggatgcg gccgtcggcg gcctgcagta gcgctggctc ggagaagtcg
     1201 gcggtggcgt cggcggcgtc ctcgacgcgg cgcgccaggt gccattccct gccttgcggg
     1261 tcgcccagcc agagcgccag gcggccgcga ccagtgccct cgttgccggc cagcagcagg
     1321 cgaccgctgg tcaggcgcag cagcgccagc ggcgtgccgg ggttggcgac ggtcggggcg
     1381 ggcgccgttt gccaggtttg accgccgtcg tcgctgcgtg cgccagccag accggggaag
     1441 gccgccagca gcgcggtcgc gctgctggtg tcggtggcgg cgatggccgg ataagcgctg
     1501 ccggtcggca ggcggcgctg gtcgacgatg cgtccggcct ggtcgacgcg tagccaagca
     1561 ccgtgcgggc gcaggccgga ctggctgagc ggcaggccga agccgccgtc ggccagcggc
     1621 agcggcgctt gcgccagcgg gg
//
LOCUS       DAOSDX010000725         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24915_length_1640_cov_2.541325, whole genome shotgun sequence.
ACCESSION   DAOSDX010000725 DAOSDX010000000
VERSION     DAOSDX010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24915_length_1640_cov_2.541325"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLN02_13610"
     CDS             complement(<1..400)
                     /locus_tag="PLN02_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53411.1"
                     /translation="MSNPELAAWLASPQGSYVLDWEVAQIDKAVSDIFGFNALQIGLP
                     QCDLLRANRMAQRLKIGAGDPVAVRCSPAVAGARPSNSSEASMRTCRDSAAGSMPPPG
                     RSLAALAMPPTNRQDDSNSMRIGFTAAPDNR"
     gene            429..1187
                     /gene="gloB"
                     /locus_tag="PLN02_13615"
     CDS             429..1187
                     /gene="gloB"
                     /locus_tag="PLN02_13615"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOI53412.1"
                     /translation="MFEEISLIPAFKDNYIWLLRRDRQAFVVDPGDAAPVRARLAAEG
                     LRLAGILITHHHADHQGGVAALLAEHDCPVYAPAEESITGRTHSLRGGERLDVLGVPV
                     EVLAVPGHTLGHLAYLAADALFCGDTLFGAGCGRLFEGTPAQMQAALARIAALPETTR
                     IYCAHEYTLMNLPFAAAVEPGNPAISERTRRAAELRQAGQATVPLTLAEEKSTNPFLR
                     WAQPAVIAAALAHGAADDSPAAVFGALRAWRNSF"
     gene            complement(1193..1591)
                     /locus_tag="PLN02_13620"
     CDS             complement(1193..1591)
                     /locus_tag="PLN02_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53413.1"
                     /translation="MNKNALSVLIVDDNDMMRTILRSMLRGDEYEIVGEARNGVQAVE
                     AAERLRPAIVCMDVLMPEKNGLVAMAEIRNAYPDTEFIVITGSADPETVQEAIHHGAT
                     GFIVKPFNAARVLDAMKKAASRIRQRQPAA"
BASE COUNT          234 a          555 c          561 g          290 t
ORIGIN      
        1 tcctgttgtc tggtgccgcc gtgaatccga tgcgcatgct gttgctgtcg tcctgcctgt
       61 tcgtcggcgg catcgccagc gccgccaacg acctgcccgg cggcggcatc gacccggcgg
      121 cgctgtcgcg gcatgtgcgc atgctggcct cggacgagtt cgaaggccgc gccccggcca
      181 ccgccggcga gcagcgcacc gccaccgggt cgccggcgcc gatcttcaag cgttgcgcca
      241 tgcggttggc gcgcagcaga tcgcattgcg gcaggccgat ctgcagcgcg ttgaaaccga
      301 aaatgtcgga taccgccttg tcgatctgcg ccacctccca gtcgagcacg taactgcctt
      361 gcggcgaggc gagccaggcg gcaagttcgg gatttgacat ccgcgcggac tggttcgata
      421 gttccggcat gttcgaagaa atatcgctca ttcctgcctt caaggacaat tacatctggc
      481 tgctccggcg cgaccggcag gccttcgtcg tcgatcccgg cgatgccgca ccggtgcgcg
      541 cccgcctggc ggcggaaggc ttgcggctgg ccggcatcct gatcacccac caccacgccg
      601 accaccaggg cggcgtcgcg gcgctgctcg cggaacacga ctgcccggtc tatgcgccgg
      661 ccgaagagtc tatcacaggc cgtactcatt ccctgcgcgg tggcgagcgt ctcgacgtgc
      721 tcggcgtgcc ggtcgaggtg ctggccgttc ccggccacac gctgggccat ctcgcctacc
      781 tcgcggcgga cgcattgttc tgcggcgaca ccctgttcgg cgccggttgc ggccgcctgt
      841 tcgaaggcac gccggcgcag atgcaggccg cgctggcgcg catcgccgcc ttgccggaga
      901 cgacgcggat ctactgcgcc cacgaataca cgctgatgaa tctgcctttt gccgccgccg
      961 tcgagcccgg caacccggca atcagcgagc gtacgcgtcg ggcggcggaa ctgcgtcagg
     1021 ccggacaggc gacggtgccg ctgacgctgg ccgaggaaaa gtcgaccaat cccttcctgc
     1081 gctgggcgca gccggcggtg atcgccgcgg cgctcgccca cggtgcggcg gatgactcgc
     1141 cggccgccgt cttcggcgcg ctgcgcgcct ggcgcaacag cttctgacgg cgtcaggccg
     1201 ccggttgtcg ctggcggatg cggctggcgg ccttcttcat cgcgtcgagg acgcgcgcgg
     1261 cgttgaacgg tttgacgatg aagccggtgg cgccgtggtg gatcgcctcc tggacggttt
     1321 ccggatcggc gctgccggtg atgacgatga actcggtgtc cgggtaggcg ttccggattt
     1381 ccgccatggc gaccaggccg ttcttttccg gcatcagcac gtccatgcag acgatggcgg
     1441 ggcgcaggcg ctcggccgcc tcgacggcct gcacgccgtt gcgggcttcg ccgacgatct
     1501 cgtattcgtc gccgcgcaac atgctgcgca ggatggtgcg catcatgtcg ttgtcgtcga
     1561 cgatgaggac ggaaagcgcg ttcttgttca tggcgggcgg atcagaaaca gtacttcatg
     1621 aaatatctga gggcggacag
//
LOCUS       DAOSDX010000726         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24943_length_1638_cov_3.784586, whole genome shotgun sequence.
ACCESSION   DAOSDX010000726 DAOSDX010000000
VERSION     DAOSDX010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24943_length_1638_cov_3.784586"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLN02_13625"
     CDS             complement(<1..218)
                     /locus_tag="PLN02_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOI53414.1"
                     /translation="METVQEQGRGPLAGVKVVEFAGIGPGPFCGMLLADMGAEVTLLE
                     RAGGTFASQLFGGGRKAVANRGKKSLCI"
     gene            316..900
                     /locus_tag="PLN02_13630"
     CDS             316..900
                     /locus_tag="PLN02_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53415.1"
                     /translation="MKSRIDELLARIAALQDELDGEYRQARDDWARKKAELADELARQ
                     QRRYKTGLFRFLIRSRLLVILTAPVIYAGWIPFLLIDLFVTIYQHICFRVYRIPRVRR
                     ADYIVFDRGDLPYLNLIEKFNCFYCSYGNGVAAYTREVAARTEQYWCPIKHARRIRAA
                     HDRYPKFFDYGDAEAFQQGLNRLRRQYDDEVPHD"
     gene            complement(913..>1638)
                     /locus_tag="PLN02_13635"
     CDS             complement(913..>1638)
                     /locus_tag="PLN02_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI53416.1"
                     /translation="PSVEDWALPPRLIFCSPSSQYPTGTVLSLDRRIQLLENADHVGS
                     WIIEDDYDNELHFHERPIGSLFGLANKQRVIYLGTFSKVMFPGIRLAYLVVPEDLVEA
                     FVIGNSELYRGGRLAEQAALAEFIDEGHFTAHIKRMRSLYAERRETLLSAMQSRLGDV
                     VMPSGSQAGLQLLYRFNQPIDDTMVAAQALAEGVICRPLSLYYLNQEARQPGMNLGFA
                     AVPVERIGPATDILTRVIERHLK"
BASE COUNT          287 a          517 c          541 g          293 t
ORIGIN      
        1 tcgatgcaca gcgatttctt gccgcggttg gccaccgcct tgcggccgcc gccgaaaagt
       61 tgcgaggcga aggtgccgcc ggcgcgttca agcagcgtga cctcggcgcc catgtcggcg
      121 agcaacatgc cgcagaaggg gccggggccg atgccggcga attcgacgac cttgacgccg
      181 gccagcgggc cgcgcccttg ttcttgaact gtctccatga tcttgttttg ttggttgata
      241 tggcccgcag tatcgcacgc gtttgcctgt gccgtggggc atcgggcatg atccgggatt
      301 gaccggagaa aagcgatgaa gagcaggatc gacgaactgc tggcgcgcat tgccgcgctg
      361 caggacgagc tggacgggga gtatcgccag gcgcgcgacg actgggcgcg caagaaggcc
      421 gaactggccg acgaactggc gcgtcagcag cgccgctaca agaccgggct gttccgcttc
      481 ctgatccgtt cccggctgct ggtgatcctc accgcgccgg tcatttacgc cggctggatt
      541 cccttcctgc tgatcgacct cttcgtcacg atctaccagc acatctgctt ccgggtctat
      601 cgcataccgc gcgtccggcg cgccgactac atcgttttcg accgcggcga tctgccctac
      661 ctgaacctga tcgagaagtt caactgcttc tactgctcct acggcaacgg cgtcgccgcc
      721 tacacccgcg aggtggcggc gcgcaccgag cagtactggt gcccgatcaa gcacgcgcga
      781 cggatcaggg cagcgcacga ccgctacccg aaattcttcg attacggcga tgccgaagcc
      841 ttccagcagg gcctgaaccg cctgcgccgg caatacgacg acgaggtgcc gcacgactga
      901 gcgcgctgtg ccctatttca ggtgtcgttc gatgacgcgc gtcaggatgt cggtggccgg
      961 gccgatccgt tccaccggga cggcggcgaa gcccaggttc atgcccggct ggcgcgcttc
     1021 ctgattgagg tagtaaagcg acagggggcg gcagatgacg ccttcggcga gcgcctgggc
     1081 ggcgaccatg gtgtcgtcga tcggctggtt gaagcggtag agcaattgca acccggcttg
     1141 gctgccggat ggcatgacga cgtcgccgag tcgcgactgc atggcgctca gcagggtttc
     1201 gcggcgctcc gcgtagaggc tgcgcatgcg cttgatgtgc gcggtgaagt gcccctcgtc
     1261 gatgaactcc gccagtgccg cctgctcggc caggcggccg ccgcgataga gctcggaatt
     1321 gccgatcacg aaagcttcga ccagatcctc gggaaccacc agataagcca gccggatgcc
     1381 ggggaacatg accttgctga aagtccccag atagatcacc cgctgcttgt tggccagacc
     1441 gaacagcgag ccgatcggcc gttcgtggaa gtgcagttcg ttgtcgtagt cgtcctcgat
     1501 gatccatgag ccgacatgat cggcgttctc cagcaactgg atgcggcggt cgagcgagag
     1561 cacggtgccg gtcgggtact ggctcgacgg cgaacagaag atcaggcgcg gcggcagcgc
     1621 ccagtcctcg acactggg
//
LOCUS       DAOSDX010000727         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_24953_length_1638_cov_2.440935, whole genome shotgun sequence.
ACCESSION   DAOSDX010000727 DAOSDX010000000
VERSION     DAOSDX010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24953_length_1638_cov_2.440935"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLN02_13640"
     CDS             <1..502
                     /locus_tag="PLN02_13640"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOI53417.1"
                     /translation="HLGDKEAMDRIRQIRQMDERHHLTLMVRDLSEIATYARVDNAQY
                     RLLKATTPGCYVFILEGSKELPRRVLHPKRKTIGLRVPAHAVALALLEELNEPLLTTT
                     LQLPGDDAPLTEGWEIQDRLDEQLELILDSGPCGIEPTTVVDLTGPSPELVRAGRGPL
                     APFGLD"
     gene            514..1161
                     /locus_tag="PLN02_13645"
     CDS             514..1161
                     /locus_tag="PLN02_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOI53418.1"
                     /translation="MNALIQTIAIAALPVVFAITLHEAAHGYAARHFGDPTAWQLGRI
                     SLNPLRHIDPVGTVLIPLAILIFSAGNFLFGYAKPVPVYFTRLRQPKKDMLWVAAAGP
                     AANLAMACFWALMFKLSWVVPDFFAVPLARMAEIGIIINCVLMVLNLLPLPPLDGGRI
                     AVSLLPHRLAWQFSRIEPWGFPILLVLLFTGILGDIMNPLVLFTAHAIEFLFGLY"
     gene            1174..>1638
                     /locus_tag="PLN02_13650"
     CDS             1174..>1638
                     /locus_tag="PLN02_13650"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926569.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOI53419.1"
                     /translation="MYAERVLSGMRPTGALHLGHYHGVLKNWVRLQHEYPCLFFVADW
                     HALTTDYDEPGAIAKNAWDMVIDWLAAGVDPAQATIFIQSRVPEHAELHLLMSMMTPL
                     GWLERVPTYKDQQEKLTHKDLTTYGFLGYPLLMSSDILIYRADKVPVGEDQIP"
BASE COUNT          265 a          558 c          498 g          317 t
ORIGIN      
        1 ccatctcggc gacaaggagg cgatggaccg catccgccag atccggcaga tggacgagcg
       61 ccatcatctg acgctgatgg tgcgcgacct ctcggagatt gccacctacg ctcgtgtcga
      121 taacgcgcag taccgcttgc tcaaggcgac gacgcccggt tgctacgtct tcatcctcga
      181 aggcagcaag gagttgccgc gccgcgtgct gcatccgaag cgcaagacga tcggcctgcg
      241 cgtgccggcg cacgcggtgg cgctcgcgct gctcgaggag ctgaacgagc ccttgctgac
      301 gaccacgctg cagttgccgg gcgacgacgc gccgctcacc gagggctggg aaatccagga
      361 tcgcctcgac gagcagctcg aactaatcct cgacagcggc ccctgcggca tcgaaccgac
      421 cactgtcgtt gacctgaccg ggccttcgcc cgagctggtt cgcgccgggc gcgggccgct
      481 ggcgcccttt gggctggatt gaacatgctc gacatcaatg cgctgatcca gaccatcgcc
      541 atcgccgcgc tgccggtggt tttcgcgatc accctgcacg aggcggcgca cggttacgcg
      601 gcccgccatt tcggcgatcc gaccgcctgg cagctcgggc gcatcagcct caatccactg
      661 cgtcacatcg atccggttgg taccgtgctg attccgctgg cgatcctgat tttttcggca
      721 ggcaacttcc tgtttggtta cgccaagccg gtgccggtgt atttcacccg gctgcgccag
      781 cccaagaagg acatgctctg ggttgccgcc gccgggccgg ccgccaacct ggcgatggcc
      841 tgtttctggg cgctgatgtt caagctgtcg tgggtggtgc cggatttctt cgcggtgccg
      901 ctggcgcgca tggccgagat cggcatcatc atcaactgcg tgctgatggt cctcaacctg
      961 ctgccgttgc cgccgctcga cggcgggcgg attgccgtca gcctgttgcc gcaccggctg
     1021 gcctggcagt tctcgcgcat cgagccgtgg ggtttcccga ttttgctggt cctgctgttc
     1081 accggtatcc tcggcgacat catgaatcct ctggtgctct tcacggcgca cgccatcgaa
     1141 ttccttttcg gtctttacta aacgacaacc gctatgtacg cagaacgtgt cctttccggc
     1201 atgcgtccca ccggggcgct gcatctcggc cattaccacg gcgtcctcaa gaactgggtc
     1261 cgcctgcagc acgagtatcc ctgccttttc ttcgtcgccg actggcacgc gctgaccacc
     1321 gactacgacg agccgggcgc tatcgccaag aacgcctggg acatggtgat cgactggctg
     1381 gcggcgggcg tcgatccagc ccaggcgacc atcttcatcc agtcgcgcgt gcccgagcac
     1441 gccgaactgc acctgctgat gtcgatgatg acgccgctgg gctggctcga acgcgtgccg
     1501 acctacaagg accagcagga aaagctcacc cacaaggatc tgaccaccta cggcttcctc
     1561 ggctacccgc tgctgatgag ctcggacatc ctgatctacc gcgccgacaa ggtgccggtc
     1621 ggcgaggacc agattccg
//
LOCUS       DAOSDX010000728         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25022_length_1634_cov_3.223559, whole genome shotgun sequence.
ACCESSION   DAOSDX010000728 DAOSDX010000000
VERSION     DAOSDX010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25022_length_1634_cov_3.223559"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PLN02_13655"
     CDS             complement(<1..160)
                     /locus_tag="PLN02_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HOI53420.1"
                     /translation="MDITELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPMEH
                     KDVHGMVYD"
     gene            216..905
                     /locus_tag="PLN02_13660"
     CDS             216..905
                     /locus_tag="PLN02_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOI53421.1"
                     /translation="MSAIADNLQAVRIRIATAERLYGRPSGSVQLLAVSKTKPLADVL
                     AARAAGQTAFGENYVQEGVEKCRTSAGQALEWHFIGPLQTNKTRPVAENFAWVHSIDR
                     LKIAERLSAQRPPELPPLQVCVQVNVSGETSKSGCAPATARDLCHAVATLPNLRLRGL
                     MAIPEPSDDFAAQRRPFRQLRELLEQLRAEGLPLDTLSMGMTHDLEAAIAEGATLVRI
                     GTAIFGERNYA"
     gene            902..>1634
                     /gene="proC"
                     /locus_tag="PLN02_13665"
     CDS             902..>1634
                     /gene="proC"
                     /locus_tag="PLN02_13665"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOI53422.1"
                     /translation="MKILFLGGGNMANALIGGMVKQGFAATDIHVIDPGSEARQRLTE
                     SYGVNCHAAAGDAPAAPDVLVLAVKPQQMREAVAPLAGRLGDALVISIAAGLGMAALA
                     RWLGDHRRIVRCMPNTPALVGAGITGLCALPEVDAAGRALADRVLRAVGSTVWIDDEA
                     QMDGVTALSGSGPAYVFLFIEALQQAADELGFSAEQGRQLAIETVRGAAALAAASNDP
                     AALLRERVTSKGGTTEAALQVMLARG"
BASE COUNT          281 a          563 c          519 g          271 t
ORIGIN      
        1 tgtcgtacac catgccgtgc acgtccttgt gctccatcgg cggcaggttg atgcgccgga
       61 cgtcgccatg gacgcgaatc atcggcggca gcccggccga aaggtgcagg tcggaagcct
      121 tgttcttgac gctgaaggcc agcagttcgg tgatgtccat gcgatcggtc ctcggagccc
      181 gtgtatactt tggtgacaaa aattgccaat ggattatgag cgcaatcgcc gacaacctgc
      241 aagctgtacg catacgaatt gccactgccg agcgccttta cgggcgccct tccggcagtg
      301 tgcaactgct cgcggtgagc aagaccaaac cgctggccga cgtccttgcc gctcgtgccg
      361 cgggacaaac cgccttcggc gagaattatg ttcaggaagg cgtcgagaaa tgccgcacca
      421 gcgccggcca ggcactcgaa tggcatttca tcggccccct gcaaaccaac aagacccggc
      481 ctgtcgccga aaacttcgcc tgggtccatt caatcgaccg cctgaagatc gccgaacgcc
      541 tgtccgcgca gcgcccaccc gagctaccgc cgctgcaggt ctgcgtgcag gtcaatgttt
      601 ccggcgagac gagcaagagc ggttgcgcgc cggccacggc gcgcgatctc tgccacgccg
      661 tcgccaccct tccgaatctg cgcctgcgcg gcctgatggc gatccccgaa ccgagcgacg
      721 acttcgcagc gcagcgccgc cccttccgcc agttgcgcga actcctggaa caattgcgtg
      781 ccgaaggact gccgctcgac acgctgtcca tgggcatgac ccacgatctc gaggccgcga
      841 tcgccgaagg cgccaccctg gtgcgcatcg gcacggccat ttttggtgaa agaaactacg
      901 catgaaaatc cttttcctgg gcggtggcaa catggccaac gcgctgatcg gcggcatggt
      961 caagcagggc tttgccgcta ccgacatcca cgtcatcgat cccggcagcg aggcgcgcca
     1021 gcgcctcacc gaaagctatg gtgtcaattg tcacgccgct gccggcgacg ctccggcagc
     1081 gccggacgtg ctagtgctgg cggtcaaacc acagcagatg cgggaagccg tcgcgccgct
     1141 tgccggccgt cttggcgatg cgctggtcat cagcatcgcc gccggtctcg gcatggcggc
     1201 gctggcccgc tggctcggcg accaccgacg catcgtccgc tgcatgccga atacgccggc
     1261 tctggtcggt gccggcatca ccggtctgtg cgcgctgccc gaggtcgacg ccgccggccg
     1321 ggcactcgcc gaccgcgtcc tgcgtgccgt cggcagcacc gtctggatcg acgacgaagc
     1381 gcagatggac ggcgtcaccg cgctttccgg cagcggcccg gcctatgtct tcctgttcat
     1441 cgaggcactg caacaggcgg cagacgaact tggtttcagc gccgagcagg gacgccagct
     1501 cgccatcgag accgttcgtg gtgcggcggc gctggccgcc gcctccaacg acccggctgc
     1561 gctgctgcgc gagcgggtga cctcgaaggg cggtaccacc gaagcggcgc tgcaggtgat
     1621 gctggcgcgc ggcg
//
LOCUS       DAOSDX010000729         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25027_length_1634_cov_2.784041, whole genome shotgun sequence.
ACCESSION   DAOSDX010000729 DAOSDX010000000
VERSION     DAOSDX010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25027_length_1634_cov_2.784041"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..456
                     /locus_tag="PLN02_13670"
     CDS             145..456
                     /locus_tag="PLN02_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53423.1"
                     /translation="MDKTMETAQRLARFKLQVKRTLGQTVDLERLTRDAGYARQRLAE
                     IEEAAEDEELLVQLLQLRDLLLPAASVPSAAPATPATAAEPATPRTAPLRNYLQGARA
                     W"
     gene            519..971
                     /locus_tag="PLN02_13675"
     CDS             519..971
                     /locus_tag="PLN02_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOI53424.1"
                     /translation="MRLSRPLTVALLSALLLAACGEAEDTRPGQPVKTRQTAFKEIIK
                     VFEPMGVMLREKRYEEEKFLRLASALHEKRDGPWAHFGPDTNYPPTKATPLVWQEPER
                     FARERDSFLKATDELLAAARSKDQARIQAAYDAVHASCQDCHKPFKTR"
     gene            976..>1634
                     /locus_tag="PLN02_13680"
     CDS             976..>1634
                     /locus_tag="PLN02_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53425.1"
                     /translation="MLRLTELKLPLDHPPAALRPALCARLGIADGELVDFRIVKRSHD
                     ARKKSAIVLIYQIDVELRDEAAVAARLADDRHVVPAPDMDYHFVAHAPEGLSERPVII
                     GMGPCGLMAGLVLAQMGFRPIILERGKAVRERTKDTWGLWRKHQLDPESNVQFGEGGA
                     GTFSDGKLYSQIKDPKFLGRKVMTEFVKAGAPEEILYLSKPHIGTFRLVKMVENMRAT
                     IR"
BASE COUNT          303 a          591 c          517 g          223 t
ORIGIN      
        1 aaaccattgc cggacaatgg ccttgacggc aagccgctga tttttcaggc cccttgctgc
       61 agcgccgcaa ccggcacgat tctcgctaat ctccagttac cccgacccca acgggacagc
      121 cagaccagaa caggagaaca gcgcatggac aagacgatgg aaaccgcgca gcgactcgcc
      181 cgcttcaagc tgcaggtcaa gcgcacgctg ggccagaccg tcgacctcga acgcctgacc
      241 cgcgacgccg gctacgcccg gcagcggctg gccgaaatcg aggaagcggc cgaggacgag
      301 gaactcctgg tccagctcct gcagttgcgc gacctgctgc tgccggcagc cagcgtgccg
      361 agcgcagccc cagcgacccc ggctaccgcc gccgagccgg caacgccgcg cacggcgccg
      421 ctgcgcaact acctgcaggg cgcccgcgcc tggtaggccg gcggcacgcc cggcggcggg
      481 tataatccgg ccttcgtcaa agccatcccc gtaccgccat gcgtctttcc cggcccctta
      541 ccgtcgccct gctttccgcc cttctcctcg ccgcctgcgg cgaagccgag gacacccgcc
      601 ccggccagcc ggtcaagacc cggcagaccg cgttcaagga gatcatcaag gtcttcgagc
      661 cgatgggcgt gatgctgcgg gaaaagcgct acgaggaaga gaagttcctg cgcctggcga
      721 gcgcgctgca cgagaagcgc gacggcccct gggcgcattt cggccccgac accaattacc
      781 cgccgaccaa ggcgacgccg ctggtctggc aggagccgga gcgcttcgca cgcgagcgcg
      841 acagcttcct gaaggccacc gacgaactgc tggctgccgc gcgaagcaag gatcaggcgc
      901 gcatccaggc cgcctacgac gcggtgcacg ccagttgcca ggactgccac aagccgttca
      961 agacgcgctg acaccatgct gcgcctgacc gaactcaagc tgccgctcga ccatccgccg
     1021 gcagcgctgc gcccggcgct gtgcgcccgc ctggggatcg ccgacggcga gctggtcgat
     1081 ttccgcatcg tcaagcgcag ccacgacgcg cgcaagaagt cggcgatcgt cctcatctac
     1141 cagatcgatg tcgaactgcg cgacgaggcc gccgtcgccg cccgtctggc tgatgaccgc
     1201 cacgtcgtgc cggcgccgga catggattac cacttcgtcg cccacgcccc ggagggactg
     1261 agcgagcgcc cggtgatcat cggcatgggc ccctgcggcc tgatggccgg gctggtactg
     1321 gcgcagatgg gcttccggcc gatcatcctc gaacgcggca aggcggtgcg cgagcgcacc
     1381 aaggacacct gggggctgtg gcgcaagcac cagctcgacc cggaatcgaa tgtccagttc
     1441 ggcgaaggcg gcgccggcac cttttccgac ggcaagctgt acagtcagat caaggacccg
     1501 aagttccttg gccgcaaggt gatgaccgag ttcgtcaagg ccggcgcgcc ggaagagatc
     1561 ctctacctga gcaagccgca catcggcacc ttccgcctgg tcaagatggt cgagaacatg
     1621 cgtgcgacga tccg
//
LOCUS       DAOSDX010000730         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25030_length_1634_cov_2.706143, whole genome shotgun sequence.
ACCESSION   DAOSDX010000730 DAOSDX010000000
VERSION     DAOSDX010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25030_length_1634_cov_2.706143"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..769)
                     /gene="mdcE"
                     /locus_tag="PLN02_13685"
     CDS             complement(47..769)
                     /gene="mdcE"
                     /locus_tag="PLN02_13685"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044262 - cellular carbohydrate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HOI53426.1"
                     /translation="MTLNTILDALFPAGHTVAVANQVLTGEAQTALGPVAVLGTTDAA
                     AIDHAMALALAGAVLDTVDRHPGRPLVFLVDTSGQALSRSQELLCLNGSLAHLASCVD
                     FARRQGHPSLALVTANAVSGGFLSFGLMADRAYALADAQVRVMDLRAMARVTKIAHER
                     LVELAASSPTFAPGAENYQKMGAIAAIWPAPTAILLLEALREVAQSRDGGDQRQTLGT
                     ARGGRQLAAGVSGQVQHAVFAG"
     gene            complement(766..>1634)
                     /locus_tag="PLN02_13690"
     CDS             complement(766..>1634)
                     /locus_tag="PLN02_13690"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0044262 - cellular carbohydrate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HOI53427.1"
                     /translation="EATARQRLAGLLDPGSFCEFLPPAVAQPSPHLAQLDLPGAFDDG
                     VIVGAGQLAGRKVLVAAQEGQFMGGAVGEIHGAKIVGLLRRAVTEKPAALLFLIDSGG
                     VRLHEANAGLIAISEIMRAVLDARAAGVPVLALLGGSCGAFGGMGIVAKLCSAVVMSE
                     EGRLALSGPEVIETVAGVEEFDAKDRALVWRVTGGKHRYLMDDCAALVDDDIAAFRNG
                     ASALLAGWQERPNTLASLRAEQVRLQGRLATYAGCRDGLEVWAALGVAEAERVPLLDC
                     DELIALKEGLPT"
BASE COUNT          276 a          568 c          549 g          241 t
ORIGIN      
        1 ggctgcctcc ggctgcagat aaaccaagtc gtggcgcaac atgctgtcag ccggcgaaca
       61 ccgcatgctg cacctgtccg ctcacgccgg ccgccagttg ccgcccgcca cgcgccgtgc
      121 cgagggtttg gcgttggtcg ccgccatccc ggctctgagc gacttcgcgc aatgcctcga
      181 gcaatagtat cgccgtcggt gccggccaga tcgccgcgat cgcgcccatt ttctggtagt
      241 tttccgctcc cggggcgaag gtcggcgaac tcgccgccag ttcgaccagc cgttcgtggg
      301 cgatcttggt cacccgcgcc attgcccgca agtccatcac ccggacctgg gcatcggcca
      361 gcgcataggc ccggtcggcc atcaggccga aggacaggaa accgccgctg accgcgttgg
      421 cggtgaccag ggcgagcgag ggatgcccct ggcgacgggc gaaatcgaca caactggcga
      481 gatgcgccag cgaaccgttc aggcagagca gttcctggct gcgcgacagc gcctggccgc
      541 tggtgtcgac taggaagacc agcggccgtc cgggatgccg gtcgaccgta tcgagaaccg
      601 cgccggccag cgccagcgcc atcgcatgat cgatcgccgc agcgtcggtg gtgccaagca
      661 ccgcgaccgg gcccagggca gtctgcgcct cgccggtaag cacctggttg gccacggcga
      721 ccgtgtgacc ggccgggaac agcgcatcga gaatggtatt cagcgtcatg tcggcaatcc
      781 ttccttcaag gcaatcagct cgtcgcagtc gagcagcggt acgcgttccg cctcggcgac
      841 gccgagcgcc gcccagactt cgaggccgtc gcggcaaccg gcgtaggtgg ccagtcggcc
      901 ttgcagacgc acctgttcgg cgcgcaggct ggccagcgtg ttcggccttt cctgccagcc
      961 ggcgaggagc gcgctggcgc cgttgcggaa agcggcgata tcgtcgtcga ccagcgccgc
     1021 gcaatcgtcc atcaggtagc ggtgcttgcc accggtgacg cgccagacca gggcacggtc
     1081 cttggcatcg aattcctcga cgccggccac cgtttcgatg acctccgggc cggacagcgc
     1141 cagccgcccc tcttcagaca tgaccaccgc cgaacacagc ttggcgacga tgcccatgcc
     1201 gccgaaggcg ccgcaactgc caccgagcag tgccaacacc ggcacaccgg cggcccgcgc
     1261 atcaagcacg gcgcgcatga tttcggagat ggcgatcagc ccggcgttgg cctcgtgcaa
     1321 gcggacgccg ccggaatcga tgagaaacag cagggctgct ggtttttcgg taacggcgcg
     1381 gcgcagcaga ccgacaatct tggcgccgtg gatttcgccg acggcaccgc ccatgaactg
     1441 gccttcctga gcagcgacca ggacctttcg gccggccaac tggccggcgc cgacgatgac
     1501 tccgtcgtcg aaagcgccgg gcaggtcgag ctgggccagg tgcggactcg gttgcgcgac
     1561 ggccggtggc aggaattcgc aaaagctgcc ggggtcgagc agaccggcca gacgctggcg
     1621 ggcggtggcc tcga
//
LOCUS       DAOSDX010000731         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25044_length_1633_cov_3.335868, whole genome shotgun sequence.
ACCESSION   DAOSDX010000731 DAOSDX010000000
VERSION     DAOSDX010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25044_length_1633_cov_3.335868"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>378
                     /locus_tag="PLN02_13695"
     CDS             <1..>378
                     /locus_tag="PLN02_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI53428.1"
                     /translation="RVASVPIKFGERLTLRVMKSDPGRQTVDALGMPPFMVERLREAL
                     ANPHGIVLVTGPTGSGKSTTLYAVLRELDADTLNILTAEDPVEQVIPGISQVQVGGKV
                     GFADALRSFLRHDPDVILVGEMRD"
     assembly_gap    379..388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <389..885
                     /locus_tag="PLN02_13700"
     CDS             <389..885
                     /locus_tag="PLN02_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOI53429.1"
                     /translation="ADVALKAATTGHMVLSTLHTNTAVGAITRLSDIGCERFLIGATL
                     HGIIAQRLVRMLCPHCRGRRPASAEERALLQVAADEPCEIGVANGCPRCLGSGYRGRI
                     GLFEALWLDGELAELVANDASERELALAAGNYWRLGDDARDKVLQAVTTLSEVRPYLH
                     LRRS"
     gene            887..>1633
                     /locus_tag="PLN02_13705"
     CDS             887..>1633
                     /locus_tag="PLN02_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOI53430.1"
                     /translation="MATFSYLGLNAQGQERAGQLHAQDLNEARQLLRGQGLRILELQA
                     GERVSRNLLDGLRDVGRLLRGMLPVRTTDMMLFYRQMQLMLRAGHTILEALEAAGRLS
                     SRPRLSDQLQRCSERISAGSSFSAALDKEKSAFPRLTVKLAEAGEASGELDSVFERLA
                     VLTERRADVKRQLLTALTYPSIVLLVSIGVISFLVGTVVPRFALFLEARGKAIPWAAQ
                     TMLDVADWLANWGVLLGVGALGPNSFLDGLT"
BASE COUNT          248 a          592 c          538 g          245 t
ORIGIN      
        1 cgcgtcgcct cggtaccgat caagttcggc gaacgcctga ccctgcgcgt gatgaagagc
       61 gaccccgggc ggcagacggt ggacgcgctg ggcatgccgc cgttcatggt cgagcgcctg
      121 cgcgaggccc tggccaaccc gcacggcatc gtcctggtca ccgggccgac cggcagcggc
      181 aagagcacga cgctctacgc cgtgctgcgc gaactcgacg ccgacacgct caacatcctc
      241 accgccgaag acccggtcga gcaggtgatt cccggcatct cgcaggtgca ggtgggcggc
      301 aaggtcggct tcgccgacgc gctgcgcagc ttcctgcggc acgacccgga cgtcatcctg
      361 gtcggcgaaa tgcgcgacnn nnnnnnnncc gccgacgtcg cgctcaaggc ggcgaccacc
      421 ggccacatgg tgctgtcgac attgcacacc aacaccgccg tcggcgccat cacccggctg
      481 tccgacatcg gctgcgagcg cttcctgatc ggtgcgacgc tgcacggcat catcgcccag
      541 cgcctggtgc gcatgctttg cccgcactgc cgcggccggc gcccggccag cgccgaggaa
      601 cgggcgctgc tccaggtggc cgccgacgag ccctgcgaga tcggcgtcgc caacggctgt
      661 ccgcgttgcc tgggcagcgg ctaccgcggc cgcatcggcc tcttcgaggc gctctggctg
      721 gacggcgaac tggccgagct ggtcgccaac gacgccagcg aacgcgaact ggcgctggct
      781 gccggcaact actggcgact cggcgacgac gcccgcgaca aggtgctgca agcggtcacc
      841 accctgagcg aggtgcgccc ctatctgcac ctgaggcggt cctgagatgg cgaccttcag
      901 ctacctcggc ctcaacgcgc agggacagga gcgcgccggg cagttgcatg cccaggacct
      961 caacgaggcg cgccagttgc tgcgcggcca gggcctgcgc atcctcgaac tgcaggccgg
     1021 cgaacgcgtc agccgcaacc tgctcgacgg cctgcgcgat gtcggccgcc tgctgcgcgg
     1081 catgctgccg gtgcgcacca ccgacatgat gctgttctac cgccagatgc agttgatgct
     1141 gcgcgccggc cacaccatcc tggaagcgct agaagccgcc ggccgcctgt ccagtcggcc
     1201 gcgcctgagc gaccagttgc aacgctgcag cgagcggatc agcgccggca gttcgttctc
     1261 ggcggcgctg gacaaggaaa aatcggcctt tccccggctg accgtgaaac tggccgaagc
     1321 cggcgaggct tccggcgaac tggattcggt tttcgagcgc ttggcggtat tgaccgagcg
     1381 ccgcgccgac gtcaaacgcc agttgctcac cgcgctgacc tacccgtcga tcgtgctgct
     1441 cgtctcgatc ggcgtcatca gcttcctggt cggcaccgtc gtgccgcgct tcgcgctctt
     1501 tctcgaagcg cgcggcaagg ccattccctg ggcggcgcaa accatgctcg acgtcgccga
     1561 ctggctcgcc aactggggcg tgctgctcgg cgtcggcgcg ctcggcccga acagtttcct
     1621 tgacgggctg acc
//
LOCUS       DAOSDX010000732         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25046_length_1633_cov_3.204689, whole genome shotgun sequence.
ACCESSION   DAOSDX010000732 DAOSDX010000000
VERSION     DAOSDX010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25046_length_1633_cov_3.204689"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1360)
                     /locus_tag="PLN02_13710"
     CDS             complement(8..1360)
                     /locus_tag="PLN02_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI53431.1"
                     /translation="MLLLIDLAVYWHGWPGQRVFATWFAAAHWRGQISFLLDALSLPV
                     ATLSALIGWLVVRFSAAYLHREAGFHRYFIVLGLFLSGLQALLLAGNGLLLFLGWEMC
                     GVASFLLIGYAWQRPLATGNALFAFVSNRVGDAGLLFSLGLAAYWAGSFEWPALFDAQ
                     LSLVDLRVLVLGFAVAALAKSAQLPFTPWIARALEGPTPSSAIFYGALLVHAGVFLML
                     RLSPLLAQLPDVQVALVVIGALTALYAWLCGLVQSDVKSALIFATVFQVGLMFVAIGL
                     GWTTLATWHLCLHAAWRAWQFLLAPSWLQLTRGRPAPPPRWLVRSQTLYTAALQRFWL
                     DRLGIILLAAPTAAIARDVRALEENFIDRVIGEPGRGRAVAPERPLVQADGLPGRLLA
                     LLADFLQRIENRLLLRGRGGAAERLLREAGRWLVTLEGLLEQPRYLMMAVMATFVVIL
                     "
     gene            complement(1348..1530)
                     /locus_tag="PLN02_13715"
     CDS             complement(1348..1530)
                     /locus_tag="PLN02_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53432.1"
                     /translation="MIELLRNVADGVWLVPLLPLFAAMVVGLRVLLGRCAGDAGEVPT
                     ARLSELAALGSLACCC"
BASE COUNT          315 a          579 c          586 g          153 t
ORIGIN      
        1 ctcggcatca gaggatcacc acgaaagtcg ccatcaccgc catcatcagg tagcgcggtt
       61 gttcgagcag cccctccagc gtcaccagcc agcggccggc ttcgcgcagc aggcgctcgg
      121 cggcaccgcc gcgtccgcgc aggaggaggc ggttctcgat gcgttgcagg aaatcggcga
      181 gcagcgccag cagccggccg ggcaagccgt cggcctggac cagcgggcgc tccggcgcga
      241 ccgcccggcc gcgcccgggt tcgccgatga cgcggtcgat gaagttttct tccagcgccc
      301 gcacgtcgcg ggcgatcgcc gcggtcggcg cggcgagcag gatgatgccc aggcggtcga
      361 gccagaagcg ctgcagcgcc gccgtgtaca gcgtctggct gcgcaccagc cagcgcggcg
      421 gcggcgccgg gcggccgcgg gtcagctgca gccaggacgg cgccagcagg aactgccagg
      481 cccgccaggc agcgtgcagg cagaggtgcc aggtggccag ggtggtccag ccgaggccga
      541 tggcgacgaa catcagaccg acctggaaga ccgtggcgaa gatcagcgcc gacttgacgt
      601 cgctctgcac caggccgcac agccaggcat agagcgcggt cagcgcgccg atcacgacca
      661 gcgccacctg cacgtcgggc aactgggcga gcagcggcga caggcgcagc atcaggaaga
      721 cgccggcatg gaccagcagc gcgccgtaga agatcgccga cgacggcgtc ggtccctcca
      781 gcgcccgggc gatccacggc gtgaacggca gttgcgccga cttggccagg gcggcgacgg
      841 cgaagccgag caccagcacg cgcaggtcga ccagcgacaa ctgggcgtcg aacagcgccg
      901 gccactcgaa actgccggcc cagtaggcgg ccagcccaag cgagaagagc aggccggcgt
      961 cgccgacgcg gttgctgacg aaggcgaaca gcgcgttgcc ggtcgccagc gggcgctgcc
     1021 aggcgtagcc gatgagcagg aaggaagcga ccccgcacat ttcccaaccg aggaagagca
     1081 gcagcccgtt gccggccagc agcagcgcct gcagcccgga aaggaagaga ccgaggacga
     1141 tgaagtagcg atggaagccc gcctcgcgat gcaggtaggc ggccgagaag cgcaccacca
     1201 gccagccgat cagggcgctc agcgtcgcca ccggcagcga caaggcgtcg agcaggaagg
     1261 aaatctggcc gcgccagtgg gcggcggcaa accacgtggc gaacacccgc tggccgggcc
     1321 agccgtgcca gtacacggcg agatcgatca gcagcagcaa gcgaggctgc cgagcgccgc
     1381 cagttcgctc aagcgcgccg tcggcacttc gccggcatcg ccggcgcagc gcccgagcag
     1441 cacgcgcaga ccaaccacca tggcggcgaa cagcggcagc aagggcacca gccagacgcc
     1501 gtcggcgacg ttgcgcaaga gttcgatcat gtcacgcctc ccagcagcac ggccggcgcc
     1561 agcggcccgg attcgccggc gaaccagtcg tgcgaacgcg ccacctgcgg cagcacgcgg
     1621 ctccccgccc agg
//
LOCUS       DAOSDX010000733         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25050_length_1633_cov_2.533587, whole genome shotgun sequence.
ACCESSION   DAOSDX010000733 DAOSDX010000000
VERSION     DAOSDX010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25050_length_1633_cov_2.533587"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLN02_13720"
     CDS             complement(<1..558)
                     /locus_tag="PLN02_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53433.1"
                     /translation="MNPVDRAISDIVLIVDDVPENLSLLHDALDEAGYTVLVATNGES
                     ALQRARQSLPDVILLDALMPGIDGFEVARRLKADFATQQIPIVFMTGLTETEHVVAAF
                     AAGGADYVTKPIRPAEVLARIAAHMQNARQMKQARSALDAFGQATVAVRAADSRIVWQ
                     TPLARDLLNAYFANPETVAPEELLAW"
     gene            complement(545..>1633)
                     /locus_tag="PLN02_13725"
     CDS             complement(545..>1633)
                     /locus_tag="PLN02_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI53434.1"
                     /translation="ELQARNKGLSFDYRPAGELPAVVRADEKRLRQILINILGNAVKF
                     TVRGGVEFKVECRREMAVFEIRDSGPGIPAEDMERIFEPFVRGSTVQAGGSGVGLTIA
                     RMLTDLMGGEMQVSSTPGEGTLFRIRLFLPQVHSAQAARELPRLNRIGYVGVRRRILV
                     VDNEKVDRDMLQSVLEPLGFIVDQAASGYEALQVIPRVKPHLVFMDLGMPGIDGWETI
                     RRIRQQQLTDAQLAILSANAFDKGLDNDVGIAPEDFIVKPLKVNQLLDWLGKKLDLEW
                     VSADTPPPAPAPAEPLPLVAPDAGQVSALEEAINLGYLRGILNQLAEIEKLDARHGEF
                     VRVLRELARQFQFDAMKEILRKSAHEPR"
BASE COUNT          232 a          498 c          620 g          283 t
ORIGIN      
        1 ccaggcgagc agttcttccg gggcgacggt ttccgggttg gcgaagtagg cgttgagcag
       61 gtcgcgggcg agcggtgtct gccagacgat gcggctgtcg gcggcgcgca cggcgacggt
      121 ggcctggccg aaggcgtcga gcgcgctgcg cgcctgcttc atctggcggg cgttctgcat
      181 gtgcgcggcg atgcgcgcca ggacttcggc cgggcggatg ggcttggtca cgtagtcggc
      241 gccgccggcg gcgaaggcgg cgacgacgtg ctcggtttcg gtcagcccgg tcatgaagac
      301 gatggggatc tgctgcgtcg cgaagtcggc tttcagccgg cgggcgactt cgaaaccgtc
      361 gatgccgggc atcagcgcat cgagcaggat gacgtcgggc aggctttggc gcgcccgctg
      421 cagcgccgat tcgccgttgg tggcgacgag cacggtgtag ccggcctcgt cgagcgcgtc
      481 atggagcagc gagaggttct cggggacgtc gtcgacgatc aggacgatgt cggagatggc
      541 gcggtcaacg gggttcatgg gccgattttc gcaggatttc cttcattgcg tcgaactgga
      601 actggcgcgc cagctcgcgc aggacgcgga cgaactcgcc gtggcgggcg tcgagctttt
      661 cgatctcggc gagctggttg aggatgccgc gcaggtagcc gaggttgatc gcttcttcta
      721 gcgcgctgac ctgcccggcg tccggagcga ccagcggcag cggctcggca ggcgccgggg
      781 cgggcggcgg cgtgtcggcg ctgacccatt cgaggtcgag cttcttgccc agccagtcga
      841 gcagttgatt gaccttgagc ggcttgacga tgaagtcctc gggggcgatg ccgacatcgt
      901 tgtccagccc cttgtcgaag gcgttggcgg aaaggatggc gagctgggcg tcggtgagtt
      961 gctgctggcg gatgcggcgg atcgtttccc agccgtcgat gccgggcatg ccgaggtcca
     1021 tgaagacgag gtgcggcttg acccgcggaa tgacctgcag cgcctcgtag ccgctggccg
     1081 cctggtcgac gatgaagccg agcggttcga gcacgctctg cagcatgtcg cggtcgactt
     1141 tctcgttgtc gacgacgagg atgcgccggc gcacgccgac gtagccgatg cggttgagcc
     1201 ggggcagctc gcgcgccgcc tgcgccgaat gcacctgcgg caggaagagg cggatgcgga
     1261 acagcgtgcc ctcgccgggc gtgctcgaca cctgcatctc gccgcccatc aggtcggtga
     1321 gcatgcgggc gatggtcagg ccgacgccgc tgccgccggc ctgcaccgtg ctgccgcgga
     1381 cgaagggctc gaagatgcgt tccatgtcct cggccgggat gccggggccg gagtcgcgga
     1441 tttcgaagac ggccatctcg cgccggcatt cgaccttgaa ttcgacgccg ccgcgcaccg
     1501 tgaatttgac cgcgttgccg aggatgttga tcaggatctg gcgcagccgc ttctcgtcgg
     1561 cgcgcaccac ggcgggcagc tcgccggccg gccggtaatc gaaggacaag cccttgttgc
     1621 gcgcctgcag ctc
//
LOCUS       DAOSDX010000734         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25081_length_1631_cov_3.953680, whole genome shotgun sequence.
ACCESSION   DAOSDX010000734 DAOSDX010000000
VERSION     DAOSDX010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25081_length_1631_cov_3.953680"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLN02_13730"
     CDS             complement(<1..254)
                     /locus_tag="PLN02_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53435.1"
                     /translation="MQRPQRPSPAATDRGTRIALLFALVAQRLDSYYEHGQELTPAQG
                     AALAADWLARSRRSLTQEERLHLSRLSDDMARTMRSTLSR"
     gene            complement(258..623)
                     /locus_tag="PLN02_13735"
     CDS             complement(258..623)
                     /locus_tag="PLN02_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOI53436.1"
                     /translation="MKEINHQRLDKVVADARKAADERAKGYRERALKLYPWICGRCAR
                     EFTHANLRELTVHHRDHNHDNNPPDGSNWELLCLYCHDNEHQKQIEADRGYTDSRNNA
                     SVATHSPFAALKSLLDKDK"
     gene            complement(637..>1631)
                     /locus_tag="PLN02_13740"
     CDS             complement(637..>1631)
                     /locus_tag="PLN02_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI53437.1"
                     /translation="LRAVAAMSAAGVAEFAPWHAVGAFAQDLDNFVRGPAVKDHPLRQ
                     LSKHVWMIFSPDGFPTPENQGMMCNLTFIDTPKGLVAVDTGASVQIGEMAVRQLKKTF
                     NKPVIAIINTHYHGDHFLGNQAFVEAYGDIPIHAHAGTIEQIKGMEGNTWRRLMEQWT
                     NQATMGTKVVPPSVAVDHGQELKFGDVVLRLHHYGKAHTPSDICVEVVGDALTCVGDV
                     AMNGRIANMDDGSYAGSLKAYEALEKNTKTKIWLPGHGEAGAGVLQWNRELFAGIYES
                     CVKAVQAGQPMEEAKTLVLRDPRVASRAKETKGFDNNIGKYVNLAYLEAEAAAF"
BASE COUNT          222 a          466 c          589 g          354 t
ORIGIN      
        1 tcgcgggaga gcgtgctgcg catggtgcgc gccatgtcgt cgctgaggcg ggagagatgg
       61 agccgctctt cctgcgtcag gctgcgccgg ctgcgggcga gccagtcggc ggcgagggcg
      121 gcgccctggg ccggggtgag ttcctggccg tgttcgtagt agctgtcgag gcgctgggcg
      181 accagcgcga acagcagggc gatgcgggtt ccccggtcgg tggcggccgg gctggggcgt
      241 tgcggacgct gcatcgctta cttgtctttg tcgagcagcg atttcagcgc ggcaaacggc
      301 gaatgggtgg ccaccgacgc gttgttgcgg ctgtcggtat agccgcggtc ggcctcgatc
      361 tgcttctggt gttcgttgtc gtggcagtac aggcaaagga gttcccagtt gctgccgtcg
      421 ggcgggttgt tgtcgtggtt gtggtcgcgg tgatgcacgg tcagttcgcg caggttggcg
      481 tgcgtgaatt cgcgggcgca gcggccgcaa atccacgggt aaagcttcag cgcccgttcg
      541 cggtagccct ttgcgcgttc gtcggcggcc ttgcgggcgt cggcgacgac tttatcgagg
      601 cgttggtggt tgatctcttt catgcgtctt tccagttcag aaggcagccg cttcggcttc
      661 caggtaggcg agattgacat acttgccgat gttgttgtcg aatcccttgg tttccttggc
      721 ccggctggcg acgcgcgggt cgcgcaggac cagggttttg gcttcctcca tcggctggcc
      781 ggcctgcacg gccttgacgc agctttcgta gatgccggcg aacagttcgc ggttccattg
      841 cagcacgccg gcaccggctt cgccgtggcc gggcagccag attttcgttt tggtgttctt
      901 ttccagtgcc tcgtaggctt tcagcgaacc ggcgtaggaa ccgtcgtcca tgttggcgat
      961 gcgcccgttc attgccacgt cgccgacgca ggtcagggcg tcgccgacca cttcgacgca
     1021 gatgtcggac ggcgtgtgcg ccttgccgta atggtgcagg cgcaggacga cgtcgccgaa
     1081 tttcagttcc tggccgtggt cgaccgcaac gctcggcggc acgaccttgg tccccatcgt
     1141 tgcctggttg gtccattgct ccatcaggcg gcgccaggtg ttgccttcca tgcccttgat
     1201 ctgctcgatg gtgccggcgt gggcgtggat cgggatgtcg ccgtaggctt cgacgaaggc
     1261 ctggttaccg aggaaatggt cgccgtgata gtgggtgttg atgatggcga tgacgggttt
     1321 gttgaaggtc ttcttcaact ggcggacggc catttcgccg atctgcaccg aggcgccggt
     1381 atcgacggcg accaggccct tcggcgtgtc gatgaaggtc aggttgcaca tcatcccctg
     1441 gttttccggg gtcgggaaac cgtcgggcga gaagatcatc cagacgtgct tggacaactg
     1501 gcgcagcgga tgatccttga ccgccggccc gcgcacgaag ttgtcgaggt cctgggcgaa
     1561 ggcgccgacg gcatgccagg gggcaaattc ggcgacgcct gcggccgaca tggcggccac
     1621 ggcgcgaagg a
//
LOCUS       DAOSDX010000735         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25084_length_1631_cov_3.614848, whole genome shotgun sequence.
ACCESSION   DAOSDX010000735 DAOSDX010000000
VERSION     DAOSDX010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25084_length_1631_cov_3.614848"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..255
                     /gene="cydX"
                     /locus_tag="PLN02_13745"
     CDS             148..255
                     /gene="cydX"
                     /locus_tag="PLN02_13745"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HOI53438.1"
                     /translation="MWYFSWVLGLAMAILFAVVNALWFESQVDRAQEDK"
     gene            complement(325..882)
                     /locus_tag="PLN02_13750"
     CDS             complement(325..882)
                     /locus_tag="PLN02_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53439.1"
                     /translation="MTSPSRPLLVVEDDESYRSLLARSLQRRGFAVRTASSADEALAV
                     CAEQAPAYVVLDLNLRGESGLNLIRPLLAYAPAARIVVLTGYASIQTTVAAMRLGACH
                     YLPKPANTGDLVRALLDDNIALDTRIDEEQLSVQHLQWEYIQRKLAENDGNISATARA
                     LKMHRRTLQRKLAKRRPPDLGFAVE"
     gene            complement(879..1532)
                     /locus_tag="PLN02_13755"
     CDS             complement(879..1532)
                     /locus_tag="PLN02_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53440.1"
                     /translation="MARLQPERDAALAEAREKTLKDEWLTSIGTLAASAAHEMATPLS
                     TLNLLLEEWAEDNAFPTAYRDDLALMRRQIESCRQTLNHLSGRASQPASANRPSIAHW
                     LDAFLQAWQATHPASSLQYTLAPTLAQYAPHFDIAVESGLGHLLDHAGRGAGPLRLRA
                     EHDGGQLTISVDGAPPPDAAGPSLALAQSTLQRAGGKLRWQGQRIELDLPLQGTALP"
BASE COUNT          225 a          495 c          601 g          310 t
ORIGIN      
        1 ctgtcggtga tgttctgggc ggcactgatc ttcacgccga tcgtcatcgc ctacaccggc
       61 tgggcctacc gcgtgatgtc cggcaaggtc accgccgatt acatcgaagc caacaacaag
      121 tccgcttact gaaaggggaa agagatcatg tggtatttca gctgggtact gggcctcgcc
      181 atggccatcc tgttcgccgt cgtcaatgcc ttgtggttcg agtcgcaggt cgatcgcgcg
      241 caagaggaca aataagctct cgttgacggc ggccgccagg accgccgtcc ttgccgggac
      301 gttccgtcgg gacgtcccgg ttttttattc gacggcgaaa ccgaggtcag gcggccgccg
      361 tttggccagc ttgcgctgca gggtgcgccg gtgcatcttg agggcgcggg cggtggccga
      421 gatgttgccg tcgttttccg ccagcttgcg ctggatgtat tcccactgca ggtgctggac
      481 gctgagttgt tcctcgtcga tgcgggtgtc cagcgcgatg ttgtcgtcga gcagggcgcg
      541 caccagatcg ccggtattcg ccggcttcgg caggtaatgg caggcgccca ggcgcatcgc
      601 ggcgaccgtc gtctggatgc tggcgtaacc ggtgaggacg acgatgcggg cggccggcgc
      661 gtaggcgagc agcgggcgga tcaggttgag tccggattcg ccgcgcaggt tgaggtcgag
      721 cacgacgtag gccggtgcct gctcggcgca gacggccagg gcttcgtcgg cgctgctcgc
      781 ggtgcgtacc gcgaaaccgc gccgctgcag ggagcgggcc agtagtgagc ggtaactctc
      841 gtcgtcctcg acgacgagca gggggcggct gggggaagtc atggcaatgc ggttccttgc
      901 aaaggcagat caagttcgat gcgttggcct tgccagcgca gcttgccgcc ggcgcgttgc
      961 agcgtgctct gggcgagtgc caggctgggt ccggcggcgt ccggcggcgg tgcgccgtcg
     1021 acgctgatcg tcaattgccc gccgtcgtgc tcggcgcgca gccgcaacgg cccggcgccg
     1081 cgcccggcgt ggtcgagcag atggccgagg ccgctttcga cggcgatgtc gaaatgcggg
     1141 gcgtactgcg ccagcgtcgg cgccagcgtg tattgcaggc tgctcgccgg gtgggtggct
     1201 tgccaggctt gcaggaaggc gtcgagccag tgggcgatcg acggccggtt ggcgctggcc
     1261 ggctggctgg cgcgcccgga gaggtggttg agggtctggc ggcagctttc gatctgccgt
     1321 cgcatcaatg ccagatcgtc ccggtaggcg gtgggaaagg cattgtcctc ggcccattcc
     1381 tcaagcagca ggttgagggt ggacagcggc gtcgccattt catgggcggc gctcgccgcc
     1441 agtgtgccga tcgaggtcag ccactcatcc ttgagcgttt tttcgcgggc ttcggcgagt
     1501 gccgcgtcgc gctcgggctg cagtcgcgcc atgatggcct ccagtgcctc ccggtcttgg
     1561 agaagggcgt cgcgctcagc cttcacccgg gtaagctcgt cccgggccct gtcgcgctcc
     1621 actgcggcca g
//
LOCUS       DAOSDX010000736         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25113_length_1630_cov_2.925714, whole genome shotgun sequence.
ACCESSION   DAOSDX010000736 DAOSDX010000000
VERSION     DAOSDX010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25113_length_1630_cov_2.925714"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PLN02_13760"
     CDS             <1..1502
                     /locus_tag="PLN02_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOI53441.1"
                     /translation="RGVRVILRNNANLSTGGSATDVTDDVHPELAAAAVAAAQTVGLD
                     IAGIDVVCDTMLKPLEEQGGGIVEVNAAPGLRMHLDPSFGKGRPVGEAIVGMMYPDGP
                     DGKPDNGRIPVVAVTGTNGKTTTSRLIGRIFEANGLRVGMTSTDGIYVENRRIDDGDC
                     SGPRSARNVLAHPDVDAAVLETARGGLLREGLGFDMCDVAVVTNLGLGDHLGLNYITT
                     VHDLAVIKRVIVQNVAPTGSAVLNAADPVVAAMAPHCPGQVTFFALDPTNPVLAAHRA
                     QGKRVLYVEKDEIVCGEGRRKYRIPLSRVPLTRNGTIGFQIENVMAAVATAWALGYAW
                     ETIERALAGFVSDAATAPGRFNVFDYRGATLIADYGHNPDAMLALVGAVEAMPADKRV
                     VVISGAGDRRDEDIRQQTEILGEAFDEVVLYQDACQRGRADGEVIGLLREGLAKSQRV
                     RHIEAITGEFLAIDTALARLQAGDLCLILIDQVEEALAHIERRIAECRQ"
     gene            1490..>1630
                     /locus_tag="PLN02_13765"
     CDS             1490..>1630
                     /locus_tag="PLN02_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOI53442.1"
                     /translation="MPPVTGKVWLVGAGPGDPDLLTLKAARLIGSADAIVYDHLVGEG
                     VRA"
BASE COUNT          269 a          598 c          537 g          226 t
ORIGIN      
        1 agcgcggcgt ccgtgtcatc ctgcgcaaca acgccaacct gtcgaccggc ggcagcgcca
       61 ccgacgtcac cgacgacgtc catccggaac ttgccgctgc cgccgttgcc gcggcgcaga
      121 cggtcggcct cgacatcgcc ggcatcgacg tcgtctgcga caccatgttg aagccgctcg
      181 aagaacaggg cggcggcatc gtcgaagtca atgccgcgcc cggcctgcgc atgcacctcg
      241 atccctcgtt cggcaagggc cggccggtcg gcgaggccat cgtcggcatg atgtaccccg
      301 acggtccgga cggcaaaccg gacaacggcc gcatcccagt cgtcgcggtc accggcacca
      361 acggcaagac gacgaccagc cggctgatcg gccgcatctt cgaagccaac ggcctgcgcg
      421 tcggcatgac cagtaccgac ggcatctacg tcgagaaccg gcgtatcgac gacggcgact
      481 gctccggccc gcgcagcgcc cgcaacgtgc tcgcccaccc ggacgtcgac gcggcagtgc
      541 tggaaacggc gcgcggcggc ctgctgcgcg aggggctcgg cttcgacatg tgcgacgtcg
      601 ccgtggtcac caacctcggc ctcggcgacc acctcggtct caactacatc accaccgtgc
      661 atgacctggc ggtgatcaag cgcgtcatcg tccagaacgt cgcacccacc ggcagcgccg
      721 tgctcaacgc cgccgacccg gtggtggcgg cgatggcacc gcactgcccg ggccaggtca
      781 ccttcttcgc cctcgacccg accaacccgg tgctcgccgc gcatcgcgcc cagggcaagc
      841 gcgtgctcta tgtcgagaaa gacgagatcg tctgcggcga gggccggcgc aagtaccgta
      901 tcccgctgtc gcgcgtaccg ctgacgcgca atggcacgat cggcttccag atagagaacg
      961 tgatggccgc cgtcgccacc gcctgggcgc tcggctacgc ctgggagacg atagagcggg
     1021 cgctggccgg tttcgtcagc gacgcggcga ccgcccccgg ccgcttcaac gtcttcgact
     1081 atcgcggcgc gacgctgatc gccgactacg gccacaaccc ggacgccatg ctggcgctgg
     1141 tcggtgccgt cgaggcgatg ccggccgaca agcgggtggt cgtcatcagc ggcgccggtg
     1201 accggcgcga cgaggacatc cgccagcaga ccgagatcct cggcgaagcc ttcgacgaag
     1261 tcgtgctcta tcaggacgcc tgccagcgcg gccgcgccga cggcgaggtg atcggattgt
     1321 tgcgcgaagg tctggccaag tcgcaacggg ttcgccacat cgaggcgatc accggcgaat
     1381 tcctcgccat cgataccgcg ctcgccaggc tgcaggccgg cgacctctgc ctgatcctga
     1441 tcgaccaggt cgaggaagcg cttgctcaca tcgagcggcg catcgccgag tgccgccagt
     1501 gaccggcaag gtctggctgg tcggcgccgg ccccggcgat cccgacctgc tgacgctcaa
     1561 ggcggcgcgc ctgatcggca gcgccgacgc catcgtctac gaccacctgg tcggcgaggg
     1621 cgtgcgcgcg
//
LOCUS       DAOSDX010000737         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25130_length_1629_cov_3.406607, whole genome shotgun sequence.
ACCESSION   DAOSDX010000737 DAOSDX010000000
VERSION     DAOSDX010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25130_length_1629_cov_3.406607"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLN02_13770"
     CDS             <1..366
                     /locus_tag="PLN02_13770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOI53443.1"
                     /translation="PDDWFDCVTVAFGLRNMTHKEQALAEMRRVLRPGGRLLVLEFSQ
                     VWKPLAPVYDFYSFQVIPRIGQLVTQDSDSYRYLSESIRVHQDQETLKAMMEGVGLER
                     VEYFNMALGVVALHRGFKF"
     gene            379..954
                     /locus_tag="PLN02_13775"
     CDS             379..954
                     /locus_tag="PLN02_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53444.1"
                     /translation="MSLSDRLLLVPLNHLIAQAPWASERLSRHAGARVAIVAGPLTLR
                     LTIDGSGLLQAADAVAEPDVRIELPDDFAVRALTDRSKLMSGARLSGAADVAETLAFV
                     FRNLRWDAEGDLAAVIGDIPARRLSLAAGALADNARDGLARLGANFAEYASNEGELLA
                     DPASIKDFVSAVDALRDDLARLDKRVERLGS"
     gene            complement(1021..>1629)
                     /locus_tag="PLN02_13780"
     CDS             complement(1021..>1629)
                     /locus_tag="PLN02_13780"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOI53445.1"
                     /translation="EGPGHVPMHLIKENMDLQLEYCKEAPFYTLGPLTTDIAPGYDHI
                     TSGIGAAMIGWYGTAMLCYVTPKEHLGLPDKNDVKEGIITYKLAAHAADLAKGHPGAQ
                     IRDNALSKARYEFRWDDQFNLGLDPDKAKSFHDETLPKESAKVAHFCSMCGPHFCSMK
                     ITQDVRDFAAAQGLREEEALTRGMEAKAVEFVQTGAEVYRKV"
BASE COUNT          244 a          503 c          545 g          337 t
ORIGIN      
        1 cctgacgatt ggttcgactg cgtgacggtg gccttcggtc tgcgcaacat gacgcacaag
       61 gagcaggcgc tggccgagat gcgccgcgtg ttgcgccccg gtggccgtct gttggtgctt
      121 gagttctcgc aggtctggaa gccgctggcg ccggtctacg atttctattc cttccaggtg
      181 atcccgcgca tcggccagct ggtgacgcag gattccgaca gctaccgcta tctttccgag
      241 tcgatccgcg tccatcagga ccaggaaacg ctcaaggcga tgatggaagg cgtcggcctg
      301 gagcgggtcg aatatttcaa catggcgctc ggtgtcgtcg ccttgcaccg cggtttcaag
      361 ttctaggcgg ctggctcgat gtccctgtct gaccggctgc tgctcgtccc gctcaaccac
      421 ctgatcgcgc aggcgccctg ggcgtcagag cgcttgagcc ggcatgccgg ggcgcgggtg
      481 gcgatcgtcg ccgggccgct gaccctgcgc ctgacgatcg acggcagcgg cctgctgcag
      541 gccgccgatg ccgttgccga acccgatgtc cgcatcgaac tgccggacga tttcgcggtg
      601 cgcgcgctga ccgaccgcag caagctgatg agcggcgccc gtctgagcgg cgccgccgat
      661 gtcgcggaaa ccttggcctt tgtcttccgc aacttgcgtt gggatgccga gggcgacctg
      721 gcggcggtga tcggcgacat tccggcccgg cgcctgagtc tggccgctgg tgccctggcc
      781 gataatgctc gcgatggact agcgcggctg ggggccaatt ttgccgaata cgccagcaac
      841 gagggcgaac tgctggccga tcctgcttcc atcaaggatt tcgtttctgc ggtcgatgcc
      901 ttgcgcgacg atctggcgcg cctcgacaaa cgggtcgaac gcctgggtag ttgaagctac
      961 ctcaacggaa atggaaaagg ccgcgatcgc ggccttttct cattgcgggt cgaatttccg
     1021 tcacaccttg cgataaacct cggcgccggt ctgcacgaat tcgaccgcct tggcttccat
     1081 tccccgagtg agcgcttcct cttcccgcaa gccttgcgcc gccgcgaagt cacgcacgtc
     1141 ctgggtgatc ttcatcgagc agaagtgcgg cccgcacatc gagcagaagt gggcgacctt
     1201 ggccgattcc ttgggcaggg tctcgtcgtg gaaggacttg gctttgtccg ggtcgaggcc
     1261 gaggttgaac tggtcgtccc agcggaactc gtagcgcgcc ttgctcagcg cgttgtcgcg
     1321 gatctgcgcg ccgggatggc ccttggccag gtcggcggcg tgcgcggcga gcttgtaggt
     1381 gatgatgcct tccttgacgt cgttcttgtc cggcaggccg aggtgttcct tcggcgtcac
     1441 gtagcagagc atggcggtgc cgtaccagcc gatcatggcg gcgccgatgc cgctggtgat
     1501 gtggtcgtag cccggcgcga tgtcggtggt caaggggccc aaggtgtaga acggagcttc
     1561 cttgcagtat tcgagctgca ggtccatgtt ctccttgatg agatgcatcg gcacatggcc
     1621 cggaccttc
//
LOCUS       DAOSDX010000738         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25192_length_1626_cov_3.117759, whole genome shotgun sequence.
ACCESSION   DAOSDX010000738 DAOSDX010000000
VERSION     DAOSDX010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25192_length_1626_cov_3.117759"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..>1626)
                     /locus_tag="PLN02_13785"
     CDS             complement(105..>1626)
                     /locus_tag="PLN02_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOI53446.1"
                     /translation="LGLVSAHDQIDLLAQVGIAVLLFLVGLKLDLHHVRHIGPVALAT
                     GLGQLAFTIAGGFALILLLGKGVMEALYVAIALTFSSTIIIVKLLSDKRELDALHGRI
                     AVGFLIVQDLAVVLAMMAMSALRGGGAAAPGEVVLSLAWRILAAAALLFILMRWLLPG
                     LVKAMAHSQELLLIFAIAWGTGLAALGEGAGFSKEAGAFLAGFSLASTAYRDAINARL
                     SGIRDFLLLFFFIDLGAKLDFSTLGGEILPAVVLSLFVLIGNPLIVMAIMGYMGYRKR
                     TGFLAGLTVAQISEFSIVFVAMGISLGHVGVEALGLTTLVGLVTIALSTYMILYSHHL
                     YQRLAPFLGLFERRNPFREQTVEDERADRNAPEVVIFGLGRYGGRLAQQLQEAGIRVL
                     GIDFDPECVSAMRQAGIPVHYGDAQESGFLEELPLNDAKWVVSTLPDVDSNIAFLQAL
                     KERRFRGEVAFVARDDAHGLRLKQAGAPTVLYPMRDAVDYTVEHFVSLIRPEEEHP"
BASE COUNT          317 a          481 c          607 g          221 t
ORIGIN      
        1 caccagatgc agcggccagc cgcgctcggc ggccagccgg taggcgcggt ccacggcatg
       61 gcgggagggc gccgaaaggt cggtggcggc caggatgcag ggagtcatgg gtgttcctcc
      121 tcggggcgga tcagggagac gaaatgttcg accgtgtaat cgacggcatc gcgcatcggg
      181 tagagcacgg tcggcgcgcc agcctgcttc aggcgcaggc catgggcgtc gtcgcgggcg
      241 acgaaggcga cttcgccgcg gaagcggcgt tccttcagcg cctgcaggaa ggcgatgttg
      301 gaatcgacgt cgggcagggt gctgaccacc catttcgcgt cgttgagcgg cagttcctcg
      361 agaaagccgg actcctgggc gtcaccgtaa tgcaccggga tgcccgcctg gcgcatggcg
      421 ctgacacatt ccgggtcgaa gtcgatgccg aggacgcgga tgccggcttc ctgcaactgc
      481 tgggcgaggc ggccgccata gcggccgagg ccgaagatca cgacttccgg cgcgttgcgg
      541 tcggcgcgct cgtcttccac cgtctgttcg cggaagggat tgcgccgttc gaagagcccg
      601 aggaagggcg ccagccgctg gtagaggtgg tgcgagtaga ggatcatgta ggtcgacagg
      661 gcaatggtca ccaggccgac cagcgtcgtc aggccgagcg cttcgacccc gacatggccg
      721 agcgagatgc ccatggcgac gaagacgatg gagaattcgc tgatctgggc gacggtcagg
      781 ccggcgagga agccggtgcg cttgcggtaa cccatgtagc ccatgatcgc catgacgatc
      841 agcggattgc cgatcagcac gaaaagggag agcacgaccg cgggcaggat ttcgccgccg
      901 agcgtcgaaa aatcgagctt ggcgccgagg tcgatgaaga agaaaagcag caggaaatca
      961 cggatgccgg agaggcgcgc gttgatcgcg tcgcggtagg cggtcgaggc gagcgagaaa
     1021 ccggccagga aggcgcccgc ctccttggaa aaaccggccc cttcgccgag cgcggcgaga
     1081 ccggtgcccc aggcgatggc gaagatcagc agcagttcct gcgagtgcgc catcgccttg
     1141 accagaccgg gcagcagcca gcgcatgagg atgaagagca gcgcggcggc ggccaggatg
     1201 cgccaggcca gcgacaggac gacttcgccg ggcgccgccg cgccgccgcc gcgcaaggcg
     1261 ctcattgcca tcattgccag caccacggcg agatcctgga cgatcaggaa gccgacggca
     1321 atgcggccgt gcaaggcgtc gagttcgcgc ttgtcggaaa gcagcttgac gatgatgatg
     1381 gtgctggaaa aagtcagggc gatggcgacg tagagcgctt ccatgacgcc cttgccgagc
     1441 agcaggatga gcgcgaagcc gccggcgatg gtgaaggcga gctggccgag gccggtggcc
     1501 agcgccaccg gtccgatgtg gcggacgtga tggaggtcga gcttgaggcc gaccaggaag
     1561 agcagcacgg cgatgccgac ctgggccagc aggtcgatct ggtcgtgggc cgaaaccagg
     1621 cccagc
//
LOCUS       DAOSDX010000739         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25195_length_1626_cov_2.641630, whole genome shotgun sequence.
ACCESSION   DAOSDX010000739 DAOSDX010000000
VERSION     DAOSDX010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25195_length_1626_cov_2.641630"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PLN02_13790"
     CDS             complement(<1..435)
                     /locus_tag="PLN02_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOI53447.1"
                     /translation="MFGIRTRRELAAVVAENHQIRTQLLASEAAADELRRQLADDRAA
                     NQALLARQTLLDGVLGNIPRFGSSLAGIQQSFSGLSGQLAGEAASAREAAAESDANRA
                     AFEQIAASLHAMFGRISAAGDSVENLTRRVGEIGGIVRLIQEI"
     gene            complement(521..1390)
                     /locus_tag="PLN02_13795"
     CDS             complement(521..1390)
                     /locus_tag="PLN02_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOI53448.1"
                     /translation="MSYLLIVNLIWSFSFSLIGQYLAGQVDSDFAVLARVAVAALIFL
                     PFTVWRGLPARLAAGFWLAGALQFGITYLCLYRSFSVLTVPEVLLFTVLTPIYVTLIE
                     DALERRFNRWALFAAAVAVGGGILIRWQPLAGDFLWGFVLLQIANATFAAGQVLCRHL
                     LLHHPVELPLYRFFGHFFLGALVLVLPSFLIFGNPDKLPVTAMQWGVLLWMGLFATAL
                     GMFWWVKGSTRVDAGTLAVMNELHVPLGLLVNLLIWNRDADLAKIALGGSVILLSLAI
                     NRLGRRTSALAAS"
     gene            complement(1443..>1626)
                     /locus_tag="PLN02_13800"
     CDS             complement(1443..>1626)
                     /locus_tag="PLN02_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOI53449.1"
                     /translation="DLTLAEPLADAQAIRRAAGQCLKRIALGKRIRLLGVRAAGLQPP
                     AATQQPLAVQAELQLF"
BASE COUNT          308 a          538 c          563 g          217 t
ORIGIN      
        1 gatttcctgg atcagccgga cgatgccgcc aatctcgccg acccgccgcg tcaggttctc
       61 gacgctgtcg ccggcggcgc tgatgcgccc gaacatggcg tgcaggctgg cggcaatctg
      121 ctcgaaggcg gcgcggttgg cgtcggattc ggcggcggcc tcgcgggcac tcgcggcttc
      181 cccggccaac tgcccggaaa gaccggaaaa cgactgctgg atgccggcca gcgaactgcc
      241 gaaacgggga atgttgccga gcacgccatc aagcagcgtt tgccgcgcca gcagcgcctg
      301 gttcgctgcc cggtcgtcgg cgagctggcg gcgcagctcg tcggcggcgg cctcgctggc
      361 cagcaactgc gtcctgatct ggtgattctc ggccaccact gccgccagct cgcgccgggt
      421 gcggattcca aacatgctgc ttcctgcctg tcactcaatc aatgttgagt aatttactca
      481 ggaattaagc gggcgaactt gatatccctc aaaaccgcgc tcagcttgcc gccagcgccg
      541 acgttcgccg cccgagccgg ttgatcgcca gcgacagcag gatgacgctg ccgccgaggg
      601 cgatcttggc gaggtcggcg tcgcgattcc agatcagcag attgaccagc aggccgagcg
      661 gcacatgcag ctcgttcatc accgccagcg tgccggcgtc gacgcgggtg ctgcccttga
      721 cccaccagaa catgccgagc gcggtggcga acaggcccat ccacagcagc acgccccact
      781 gcatcgccgt caccggcagc ttgtccgggt tgccgaagat gaggaaggac ggcagcacga
      841 ggaccagggc gcccaggaaa aaatggccga agaagcggta cagcggcagc tcgaccggat
      901 gatgcagcag caggtggcgg cacagtacct ggccggcggc gaaggtcgcg ttggcaatct
      961 gcagcagcac gaagccccac aggaaatcgc cggccagcgg ctgccagcgg atcaggatgc
     1021 cgccgccgac ggcgaccgcg gcggcgaaca gcgcccagcg gttgaagcgg cgttcgagcg
     1081 cgtcctcgat cagggtcacg tagatcggcg tcagcaccgt gaacagcagc acttccggca
     1141 ccgtcagcac gctgaagctg cggtacaggc agaggtaggt gatgccgaac tgcagcgccc
     1201 cggccagcca gaaaccggcc gccagccgcg ccggcaggcc gcgccagacg gtgaacggca
     1261 agaagatcag ggcggcgacg gcaacccggg caagcacggc gaagtcgcta tcgacctggc
     1321 cggcgagata ctggccgatc aggctgaagg aaaacgacca gatcaggttg acgatgagca
     1381 ggtaggacat gacggcggtc ggcggcaaaa gcgcgaatga taaaggcagg cggcgccggc
     1441 cgctaaaaga gttgcagctc ggcctgcacc gcaagcggct gctgcgtcgc cgccggcggc
     1501 tgcagcccgg cggcgcgcac gccgagcagg cggatgcgct tgccgagcgc gatgcgcttc
     1561 aggcactgcc cggccgcccg gcggatcgcc tgggcgtcgg ccagtggttc ggccagcgtc
     1621 aggtcg
//
LOCUS       DAOSDX010000740         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25251_length_1623_cov_3.673469, whole genome shotgun sequence.
ACCESSION   DAOSDX010000740 DAOSDX010000000
VERSION     DAOSDX010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25251_length_1623_cov_3.673469"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLN02_13805"
     CDS             complement(<1..168)
                     /locus_tag="PLN02_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOI53450.1"
                     /translation="MKLCGFEAGLDQPFFLIAGPCTAESEQLCVDVAGHMQEVSARLG
                     INYIFKASYDKA"
     gene            complement(165..>1623)
                     /locus_tag="PLN02_13810"
     CDS             complement(165..>1623)
                     /locus_tag="PLN02_13810"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOI53451.1"
                     /translation="TDDGAETDLDLGHYERFTSAKMGKRNNFTTGQVYDAVLKKERRG
                     EYLGKTVQVIPHITDEIKGKVKAGAEGADVAIVEVGGTVGDIESLPFLEAIRQMGIEE
                     GRNNVCYMHLTLLPYIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRSIPVEERR
                     KIALFCNVMPEAVIECLDADSIYKIPGMLHEQMLDEIVCHKLGILAKAADLSVWEKLI
                     DAMEHPLNTVRIAFVGKYVDLTESYKSLIEAIKHAGIHTRCKVDIEYIDSENIEKEGT
                     GVLETMDAILVPGGFGRRGTEGKIAAIRYARENKVPYLGICLGMQMAVVEFARDVAGM
                     KDAHSTEFVADTPFPVIGLITEWLDASGKVEKRGEDSDLGGTMRLGGQVCRLKEGSLA
                     RQIYGKAEITERHRHRYEVNNTLLSQLEAKGLVVAGRAPGTDLCEMVELPADVHPWFV
                     GCQFHPEFTSNPRQGHPLFTSYVQAALTSKQGKSA"
BASE COUNT          270 a          488 c          529 g          336 t
ORIGIN      
        1 ggccttgtcg tacgatgcct tgaaaatgta gttgatgccg agccgggcgc tgacttcctg
       61 catgtgaccg gcgacatcga cgcaaagctg ttccgattcg gcagtgcaag gaccggcgat
      121 caggaaaaaa ggctggtcaa gaccagcctc gaatccgcac agcttcatgc cgatttgccc
      181 tgtttgctgg tcagcgccgc ctgcacgtag gacgtgaaca gcgggtgacc ctgacgcggg
      241 ttggacgtga attccgggtg gaactggcag ccgacgaacc acggatggac gtcggccggc
      301 agttcgacca tctcgcaaag atcggtgccc ggcgctcggc cggcgacgac caggcccttg
      361 gcttcgagtt gcgacagcag tgtgttgttc acttcatagc gatggcggtg gcgttcggtg
      421 atttccgcct tgccgtagat ctggcgcgcc agcgaacctt ccttgaggcg gcagacctgg
      481 ccgccgaggc gcatggtgcc gccgaggtcg gaatcctcgc cgcgcttctc gaccttgccg
      541 gaagcatcga gccattcggt gatcaggccg atcaccggga agggcgtgtc ggcgacgaat
      601 tcggtcgaat gcgcgtcctt catgccggcg acgtcgcggg cgaactcgac gaccgccatc
      661 tgcatgccga gacagatgcc aaggtaaggc accttgttct cgcgggcgta gcggatcgcc
      721 gcaatcttgc cttcggtacc gcgccggccg aaaccgccgg gcaccaggat ggcgtccatg
      781 gtttcgagga cgccggtgcc ttccttctcg atgttctcgg aatcgatgta ctcgatatcg
      841 accttgcagc gcgtatggat gccggcatgc ttgatcgcct cgatcagcga cttgtaggac
      901 tcggtcagat cgacgtactt gccgacgaag gcgatgcgca ccgtgttcag cgggtgctcc
      961 atggcatcga tcagcttttc ccagaccgag aggtcggcgg ccttggccag gatgccgagc
     1021 ttgtggcaga cgatctcgtc gagcatctgc tcgtgcagca tgcccggaat cttgtagatc
     1081 gaatcggcgt ccaggcactc gatgacggct tccggcatga cgttgcagaa cagcgcgatc
     1141 ttgcggcgtt cttccaccgg gatcgaacgg tcggcacggc agagcaggat gtccggctgg
     1201 atgccgatct cgcgcaactc cttgaccgag tgctgggtcg gcttggtctt cagttcgccg
     1261 gcggtcggga tgtagggcaa gagcgtcagg tgcatgtagc agacgttgtt gcggccttcc
     1321 tcgatgccca tctggcggat cgcttccagg aagggcagcg actcgatgtc gccgaccgtg
     1381 ccgccgacct cgacgatggc cacgtcggcg ccttcggcac cggccttgac cttgcccttg
     1441 atctcgtcgg tgatgtgcgg gatgacctgc accgtcttgc cgagatactc gccgcgcctt
     1501 tccttcttca gcactgcgtc atagacctga ccggtggtga agttgttgcg cttgcccatc
     1561 ttggcgctgg tgaagcgctc gtaatggccg aggtcgaggt cggtctcggc gccgtcgtcg
     1621 gtg
//
LOCUS       DAOSDX010000741         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25253_length_1623_cov_3.115434, whole genome shotgun sequence.
ACCESSION   DAOSDX010000741 DAOSDX010000000
VERSION     DAOSDX010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25253_length_1623_cov_3.115434"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="PLN02_13815"
     CDS             complement(<1..1369)
                     /locus_tag="PLN02_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HOI53452.1"
                     /translation="MAKAKDTAKDAPKARSSKAKDKAAEKALLEGALTETPQPLDAEA
                     RKMRLKALIKLGKERGYLTYAEINDHLPDDVVDAESIEAIISTFSEMSIQVFDEAPAA
                     EDLLMSDTAASATDDEEVEAQAEQALSTVDSEFGRTTDPVRMYMREMGSVELLTREGE
                     IEIAKRIEEGLKHMIQAISACPTTIADILDMATKVEADEMRIDELIDGLIDPNAVEEA
                     PAAEAAAIDSDEEADEDEDEDGDEGGGAASASLLQLKTEACERFKSIRAIHAKMQKTL
                     TSKGSQDKAYVKLLQQLSDELMNIRFTSRSIERLCDSVRGMVEEVRGCERKIQQICVD
                     RVRMPRPHFIQSFPGNETNLDWVEAEIAAAPKAYVAILTRSAPDIQEEQKKLLALQER
                     IGISLKELKDINKQMSTGEAKARRAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEG
                     NIGLMK"
     gene            complement(1416..>1623)
                     /locus_tag="PLN02_13820"
     CDS             complement(1416..>1623)
                     /locus_tag="PLN02_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOI53453.1"
                     /translation="EALELPFDGDEGEAEAEFRAALEKLRDAAEMRDFAQLQSKAQQC
                     GVAGLSATEKQAYIQFLTTRGKRI"
BASE COUNT          230 a          481 c          518 g          394 t
ORIGIN      
        1 ccttcatcag gccgatgttg ccttcctgga tcaggtcgag gaactgcagg ccgcgattgg
       61 tgtacttctt ggcgatcgag atcaccagac gcaggttggc ttcggtcatt tcgcgcttgg
      121 cgcggcgggc cttggcttca ccggtggaca tctgcttgtt gatgtccttc agttccttga
      181 gcgagatgcc gatgcgttcc tgcagggcga gcagcttctt ctgttcttcc tggatgtccg
      241 gcgcactgcg ggtcaggatg gcgacgtagg ccttcggcgc ggcggcgatt tcggcctcga
      301 cccagtcgag gttggtttcg ttgccgggga aggactggat gaaatgcgga cgcggcatgc
      361 gcacgcggtc gacgcagatt tgctggatct tgcgctcgca gccgcggact tcctcgacca
      421 tgccgcgcac gctgtcgcac aggcgctcga tgctgcgcga ggtgaagcgg atgttcatca
      481 gctcgtcgga gagttgttgc agcagcttga cgtaggcctt gtcctgagaa cccttgctgg
      541 tcaacgtctt ctgcatcttg gcgtggatcg cgcggatcga cttgaagcgc tcgcaggctt
      601 ccgtcttcag ttgcagtagc gaggccgagg cggcgccgcc gccctcgtcg ccgtcttcat
      661 cctcatcctc gtcggcttcc tcgtcgctgt cgatggcggc ggcttcggcg gccggggctt
      721 cctcgaccgc gttggggtcg atcaggccgt cgatcagttc gtcgatgcgc atttcatcgg
      781 cttcgacctt ggtcgccatg tcgaggatgt cggcgatcgt cgtcgggcag gcggaaatcg
      841 cctggatcat gtgcttgagg ccttcctcga tgcgcttggc gatttcgatt tcgccttcgc
      901 gggtgaggag ttcgaccgag cccatctcgc gcatgtacat gcgcaccggg tcggtggtac
      961 ggccgaattc ggaatcgacg gtggataggg cctgttcggc ctgtgcttcg acttcctcgt
     1021 catcggtggc cgaggcggcg gtgtccgaca tcagcaggtc ttcggcagcc ggggcttcgt
     1081 cgaagacctg gatgctcatt tcgctgaacg tcgaaatgat cgcttcgatc gactcggcgt
     1141 caaccacatc gtccggcagg tggtcgttga tttcggcgta agtcaggtaa ccgcgttcct
     1201 tgcccagctt gatcagtgcc ttcagccgca tcttgcgggc ttcggcgtcc agcggttgcg
     1261 gggtttccgt cagcgcgcct tcgagcagcg ccttttcggc agccttgtcc ttggccttgc
     1321 tgctgcgagc cttgggagca tccttagcgg tgtcttttgc cttagccatg aaaacgatcc
     1381 gttagattgg agaaaaccgt aaattttacc acaacttaga tacgttttcc ccgggtggtg
     1441 agaaactgga tgtacgcctg cttttccgtt gccgacagtc cggcgacgcc gcactgctgc
     1501 gccttcgact ggagttgcgc gaagtcgcgc atttccgcgg catcgcgcag cttttccagg
     1561 gcggcgcgaa attccgcctc ggcctcgcct tcgtcgccgt cgaacggcag ttcgagcgct
     1621 tcc
//
LOCUS       DAOSDX010000742         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25354_length_1618_cov_3.458093, whole genome shotgun sequence.
ACCESSION   DAOSDX010000742 DAOSDX010000000
VERSION     DAOSDX010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25354_length_1618_cov_3.458093"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLN02_13825"
     CDS             <1..475
                     /locus_tag="PLN02_13825"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOI53454.1"
                     /translation="DAAFIERVQASIEKLLPTMPRQEVIRTSLSNRGAFILVRDMDEA
                     IAIANRVAPEHLELALDNPDPWVEKIRHAGAIFIGRYTSESLGDYCAGPNHVLPTSGS
                     ARFSSPLGVYDFQKRTSLIKVSQAGAQTLGRIASTLAHGEGLPAHAKSAEFRLAD"
     gene            549..1058
                     /locus_tag="PLN02_13830"
     CDS             549..1058
                     /locus_tag="PLN02_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOI53455.1"
                     /translation="MAFAAVGLSGFGGVLPFARRMLVEQRRWLSAEEFNAQLGLCQFL
                     PGPNVVNLSIVVGKRFHGWRGAIVAPLGMLLAPMLIVLLLGMAYDRWGQLPLAQDMLR
                     GIAAAGCGLLFAMAWRMGAALRDKALFLPFTVLTVAAIAWLRWPLPAVMLGGLAAAGA
                     VAWWRLGRP"
     gene            1055..1573
                     /locus_tag="PLN02_13835"
     CDS             1055..1573
                     /locus_tag="PLN02_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOI53456.1"
                     /translation="MIVVQLFLEFTLLSFVAFGGATALLPEMHRVVVDQQHWLDATTF
                     THLYAIAQAAPGPNVLVVTLIGWEIAGLAGALATTLAMCLPMSVLIYLLIDRWDSFAG
                     RRWQQALSLGVAPLAVGLIFSGATLIAQAAGLGWRGGLLVVAIMVLNLRTRWHPLWFI
                     AAGALLGLSGWL"
BASE COUNT          198 a          540 c          562 g          318 t
ORIGIN      
        1 cgacgccgcc ttcatcgagc gcgtccaggc gagcatcgag aagctgctgc cgaccatgcc
       61 gcgccaggaa gtgatccgta cctcgctgag caaccgcggc gctttcatcc tggtgcgcga
      121 catggacgag gcgattgcca tcgccaaccg cgtcgcgccg gaacacctcg agctggcgct
      181 cgacaacccg gacccgtggg tggaaaagat ccgccacgcc ggcgccatct tcatcggccg
      241 ctacacctcg gaatcgctcg gcgactactg cgccgggccg aaccacgtgc tgccgacctc
      301 cggttcggcg cgcttctcgt cgccgctcgg cgtctacgac ttccagaaac ggaccagcct
      361 gatcaaggtg tcgcaggccg gcgcgcagac gctgggccgc atcgcatcca cgctggcgca
      421 cggtgagggc ctgccggcac acgccaagtc ggccgagttc cggcttgccg actgattttc
      481 ggcaaattct gcggccgacc gtggatcacc cgccaaatcc ccagcgcccg gacctccggg
      541 cgcttttttt ggccttcgcc gcggtcggcc tgtccggttt cggcggcgtg ctgccgttcg
      601 cccggcgcat gctggtcgaa cagcggcgtt ggctgagcgc cgaggaattc aacgcccagc
      661 tcggcctctg ccagttcctg cccgggccga acgtggtgaa cctgtcgatt gtcgtcggca
      721 agcgtttcca tggctggcgc ggcgccatcg ttgcgccgct cggcatgttg ctggcgccga
      781 tgctgatcgt cctgctgctc ggcatggcct acgaccgctg gggccaactg ccgctcgccc
      841 aggacatgct gcgcggcatc gccgccgccg gttgcggtct gctcttcgcg atggcctggc
      901 ggatgggcgc ggcgctgcgc gacaaggcgc tgttcctgcc attcaccgtg ttgaccgtgg
      961 ccgccatcgc ctggctgcgt tggccgctgc cggcggtgat gctgggcggg ctggcggcgg
     1021 ccggcgcggt ggcctggtgg cgattggggc ggccatgatc gtcgtccagc tcttcctcga
     1081 attcacgctg ctttccttcg tcgcctttgg tggcgccacc gcgctgctgc cggaaatgca
     1141 ccgggtcgtc gtcgatcagc agcactggct cgatgccacc actttcaccc atctttacgc
     1201 gattgcccag gcggcgccgg gaccgaatgt gctggtggtg acgctgatcg gctgggagat
     1261 cgccggtttg gctggagcgc tggcgacgac gctggcgatg tgcctgccga tgagcgtgct
     1321 gatctacctg ttgatcgacc gctgggatag cttcgccggt cgccgctggc agcaggcgct
     1381 cagcctcggt gtggcgccgc tggcggtcgg cctgatcttt tccggggcga cgctgatcgc
     1441 ccaggcggcc gggctgggtt ggcgcggtgg cttgctggtg gtggcgatca tggtgttgaa
     1501 tctgcgtacg cgctggcatc cgttgtggtt cattgctgcc ggcgcgcttc tcggtttgtc
     1561 cggctggctg tgacggccgg cgtttgcagg agggcttggg cgagatggtg atgcaggc
//
LOCUS       DAOSDX010000743         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25373_length_1617_cov_2.923175, whole genome shotgun sequence.
ACCESSION   DAOSDX010000743 DAOSDX010000000
VERSION     DAOSDX010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25373_length_1617_cov_2.923175"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PLN02_13840"
     CDS             <1..1609
                     /locus_tag="PLN02_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOI53457.1"
                     /translation="IDGLSGDLLALPVMLLALYGSLRFSNSLFTELREILFARVTQRA
                     IRKVALEVFLHLHALSLRFHLERQTGGISRDIERGTRSISSLISYSLYSILPTLVEIS
                     LVLGILFVRYDAGFVVITVLSLVAYVLFTIKVSNWRIAIRRAVNESDSAANTRAVDSL
                     LNYETVKYFNNESWEADRYDQQLRQLENASTRSQTTLAALNLGQQAIIALGVTAMMWR
                     AASGVVAGTMTIGDLVLVNAFLIQLYLPLNFLGIVYRELRQALTDIERMFGLLNEHRE
                     IADAPDARSLPAGQAMTIDFIGVDFAYEANRQILHAVTFSVPAGSTVAVVGHSGAGKS
                     TLARLLFRFYDVTGGSIRINGNDLRQLTQHSLRSAIGIVPQDTVLFNDSILYNIRYGR
                     TDASDDEVIAAARAASLHDFVSRLPEGYATRVGERGLKLSGGEKQRVAIARALLKNPP
                     ILIFDEASSALDSATEKAIQAQLELAARGRTTLVIAHRLSTIMNADRILVMDGGCIVE
                     SGTHTELVAKNGKYANMWLLQQRESAI"
BASE COUNT          305 a          557 c          458 g          297 t
ORIGIN      
        1 gattgacggc ctctccggcg atctgctggc gctgccggtg atgttgctgg cgctctacgg
       61 aagtttgcgt ttcagcaact ccctctttac cgaactgcgc gaaatcctct tcgcccgggt
      121 cacccagcgt gcgatccgca aggtggcgct ggaggtcttc ctgcatctgc acgcgctgtc
      181 gttgcgcttt cacctggagc ggcagaccgg cggcatctcc cgcgacatcg agcgcggcac
      241 ccgctcgata tccagcctga tctcgtattc tctctactcg atcctgccga cgctggtcga
      301 aatctcgctc gtcctgggca ttctcttcgt tcgctacgat gcgggtttcg tcgtcatcac
      361 cgtgctttcg ctggtcgcct acgtgctgtt cacgatcaag gtcagcaact ggcggatcgc
      421 catccggcgc gccgtcaacg aaagtgactc agcggccaac acacgcgctg tggacagcct
      481 gctgaattac gagacggtca agtatttcaa caatgaaagc tgggaagcgg accgctacga
      541 ccagcaacta cgccaactcg agaacgccag cacgcgtagc cagaccacgc tggccgccct
      601 caacctcggc cagcaggcga tcattgccct tggcgtgacg gcgatgatgt ggcgcgcggc
      661 gagcggcgtc gtcgccggca ccatgacgat cggcgacctg gtgctggtca acgccttcct
      721 gatccagctc tatctgccgc tcaacttcct cggcatcgtc tatcgcgaac tgcgccaggc
      781 gctcaccgac atcgagcgga tgttcggctt gctcaacgaa catcgggaga tcgccgatgc
      841 gcccgacgcc cggagcttgc ccgccggaca ggcgatgacg atcgatttca tcggggtcga
      901 cttcgcctac gaggcgaacc gccagattct ccacgccgtc accttcagcg tgccggccgg
      961 cagcaccgtc gccgtcgtcg gtcatagcgg cgccggaaaa tccacgctgg cccgcctgct
     1021 gttccgtttc tacgacgtca ccggcggcag catccgcatc aacggcaacg atctgcggca
     1081 actgacccag cacagcctgc gctcggcgat cggcatcgtg ccgcaggaca ccgtactgtt
     1141 caacgactcg atcctctaca acatccgcta cgggcgcacc gacgccagcg acgatgaagt
     1201 gatcgccgcc gcccgcgccg ccagcctgca cgatttcgtc agccgcttgc ccgaaggcta
     1261 cgccacccgc gtcggcgagc gcggcctgaa actctccggc ggcgaaaagc agcgcgtcgc
     1321 catcgcccgc gccttgctca agaacccacc catcctgatt ttcgacgaag cgagttcggc
     1381 actcgactcg gcgaccgaaa aagcgatcca ggcccagctc gaactcgccg cccgcggcag
     1441 gacaacgctg gtgatcgccc atcgcctgtc cacgatcatg aacgccgatc gcattctggt
     1501 catggatggc ggctgcatcg tcgaaagcgg cacgcatact gaactggttg caaaaaatgg
     1561 aaaatatgcc aacatgtggc tgctacagca aagagagagc gccatttgat gggcgtc
//
LOCUS       DAOSDX010000744         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25416_length_1614_cov_4.177678, whole genome shotgun sequence.
ACCESSION   DAOSDX010000744 DAOSDX010000000
VERSION     DAOSDX010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25416_length_1614_cov_4.177678"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..718)
                     /locus_tag="PLN02_13845"
     CDS             complement(206..718)
                     /locus_tag="PLN02_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53458.1"
                     /translation="MGVIFAKTDKGLAELTLKSGGLTPRQRRVLIMVDGKRSVEELRE
                     LLQNDDLQHTLGILEESGFIEIDGLQNAAGDIQEAPTGPLPPIDAFRPLPVPPSAKDM
                     ELARNFIQNTLKTFCGPFAHLHIVEAAHAAKSHEELRLQFAPWYHAIVQTRDGKRRAE
                     ELRALLLRVI"
     gene            complement(826..1209)
                     /locus_tag="PLN02_13850"
     CDS             complement(826..1209)
                     /locus_tag="PLN02_13850"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOI53459.1"
                     /translation="MQRTLLKSKLHRVTATHADLHYEGSCAIDEDLLEAADIKEYEQI
                     DIWNVNNGERFTTYAIRAERGSGVISVNGSAARRAAPGDILIIATFATYTEVELVKHE
                     PSLIYVDSQNRIVRRGHKIPVQAAA"
     gene            complement(1289..>1614)
                     /locus_tag="PLN02_13855"
     CDS             complement(1289..>1614)
                     /locus_tag="PLN02_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOI53460.1"
                     /translation="PADVDTVRTGQLADIRFTGFKATTTPVVEGKLVYLAADRLVDQH
                     SGLPYFNSHIQLTAAGLSKLQQQGLQIQPGIPAEVIVKTGERTLLQYIVQPLSNVLAR
                     TFREQ"
BASE COUNT          284 a          459 c          542 g          329 t
ORIGIN      
        1 ccggcccgca agccgggccg cagccgcaag ccgaagaagg ccgaggacgg cagcgcctga
       61 tccttggggt gaaactaccc ccggtagcag cgctcgaccg ggggagttca gtaaaagggc
      121 gtgctaccgg cgattgcgca agatcagcgc acgcccccgg gaaatgcccg ccaacgggct
      181 gagaattgcc acgccggaca gtgcatcaga tgacgcgcag cagcagggcg cgcagttcct
      241 cggcgcggcg tttgccgtcg cgggtctgga cgatggcgtg ataccagggc gcgaactgca
      301 gccgcaactc ttcgtggctt ttcgcggcat gcgccgcttc gacgatgtgc agatgggcga
      361 aggggccgca aaaagtcttc agcgtgttct ggatgaagtt gcgggccagt tccatgtctt
      421 tggcgctggg tggaaccggc aacggccgga aggcatcgat gggcgggagc ggcccggtgg
      481 gcgcttcctg aatatcgcca gcggcgttct gcagtccatc gatttcaatg aaaccggatt
      541 cttcgaggat gccgagggtg tgttgcaggt catcgttctg cagcaactcc cgcaactcct
      601 cgacactgcg tttgccgtcg accatgatca gcactcgccg ctggcgcggc gtcagcccgc
      661 cggatttcag cgtcagctcg gccaggccct tgtctgtctt ggcgaaaatc acgcccatca
      721 atcgtctccg tccggttgtt aactattgtg gaacggattg tacagaaaaa gaaaagcccg
      781 ccaatgggcg ggcctcttcg gcaggacttc gcgaaagcgc agtcttcagg cggcggcctg
      841 gaccgggatc ttgtgaccgc gacggacgat gcggttctgc gaatcgacgt agatcagcga
      901 cggctcgtgt ttgacgagtt cgacttcggt atacgtggcg aaggtggcga tgatcaggat
      961 gtcgccgggc gccgcgcggc gcgctgcgga accgttgacc gagatgacgc ccgagccgcg
     1021 ttcggcacgg atggcgtagg tcgtgaagcg ctcgccgttg ttgacgttcc agatgtcgat
     1081 ctgctcgtat tccttgatgt cggccgcttc cagcaggtcc tcgtcaatgg cgcacgaacc
     1141 ttcgtaatgc agatcggcgt gcgttgccgt cacgcgatgc agcttggatt tcaacagggt
     1201 tctctgcatg gctttattca tccagacagt tggggggaag cgaattttaa cggcattgcc
     1261 ccccttgtca acgatctttt tggcggattt actgctcccg gaaggtgcgg gcgagcacgt
     1321 tgctgagcgg ttggacgatg tattgcagca gggtgcgttc gccggtcttg acgatgactt
     1381 ccgctggaat cccgggctgg atctgcaggc cctgctgttg cagcttgctc aagccggcgg
     1441 cagtgagctg gatgtgactg ttgaagtagg gcaggccgct gtgctggtcg accagacggt
     1501 cggcggccag gtagaccagc ttgccttcga ccactggcgt cgtcgtggcc ttgaaaccag
     1561 tgaagcggat atcggcgagt tgtccggtgc gcaccgtgtc gacgtctgcc ggcg
//
LOCUS       DAOSDX010000745         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25472_length_1611_cov_7.445373, whole genome shotgun sequence.
ACCESSION   DAOSDX010000745 DAOSDX010000000
VERSION     DAOSDX010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25472_length_1611_cov_7.445373"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLN02_13860"
     CDS             complement(<1..969)
                     /locus_tag="PLN02_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOI53461.1"
                     /translation="MDEITSVFVQESREQLAEMESCLLGLEQNPDDPDKINAIFRAAH
                     TIKGGAGVIECHFIEAFTHRVENVLDALRNGALGVTPALTTLLLGCCDHMLSLIDLLA
                     AGEAGPAGELQAQGEALGGQVQAFLDGIGKADEPTAASELTVSGSDPEVEIENAGAIV
                     LNDSWHVSVRFGENVMRGGVDPLSFIRYLGALGEILAIVTVTDAVPAAAAMDPESCYL
                     GFEVRLQTRSTKEDIERVFDFVRDECELRILPPNSRLSEYLKHIQELPEDSMRLGEIL
                     VRCGALTQTELEHALSDQKQGDGSASADFQPLGEILVDHKVVSPEVV"
     gene            complement(986..1291)
                     /locus_tag="PLN02_13865"
     CDS             complement(986..1291)
                     /locus_tag="PLN02_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOI53462.1"
                     /translation="MSAKDPLVVSQPLTIYHALEQKQQLLDALAATAELELNLSQVAE
                     IDAAGLQLLVLLKKEAQRCGKRARIVAHSQTVSSLIDFCDLAAELGDPLVIPAHEAV"
     gene            complement(1301..>1611)
                     /locus_tag="PLN02_13870"
     CDS             complement(1301..>1611)
                     /locus_tag="PLN02_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOI53463.1"
                     /translation="DSSQQLAAMFMEAQASVQFQDVSRQQIEQVMQALSRLDGHAGLL
                     ADRLRAFDDPAFKYEPIAEHLQALYSRYVMESQRTTHQSVLKHETAGVAPVSSRIELF
                     "
BASE COUNT          261 a          507 c          527 g          316 t
ORIGIN      
        1 gaccacttcc gggctgacca ccttgtggtc gaccaggatt tcgcccagcg gttggaaatc
       61 cgcgcttgca ctcccgtcgc cttgcttctg gtcgctcagg gcgtgctcga gttcggtctg
      121 ggtcagcgcc ccgcagcgga ccaggatttc acccaggcgc atgctgtctt caggcaattc
      181 ctggatgtgc ttcagatact cggagagccg gctgttcggc ggcaggatgc gcagttcgca
      241 ttcgtcgcgc acgaagtcga agacgcgctc gatgtcttcc ttggtactgc gggtctgcaa
      301 acggacctcg aacccgaggt aacaggattc cggatccatg gctgcggccg ccggcacggc
      361 gtcggtgacg gtcacgatgg cgaggatctc gcccagcgcg cccaggtagc ggatgaagga
      421 caagggatcg acgccgccgc gcatcacgtt ctcgccgaaa cgcaccgaaa cgtgccaact
      481 gtcgttgaga acgatggcgc cggcgttttc gatttcgacc tcggggtcgc tgccggagac
      541 cgtcagttcc gacgccgcgg tgggctcgtc ggctttgccg atgccgtcga gaaaagcctg
      601 cacctgcccg cccagcgctt cgccctgcgc ctgcagttcg cccgccggcc cggcttcgcc
      661 agccgccagc aggtcgatca ggctgagcat gtggtcgcag cagccaagca gcagtgtggt
      721 cagcgccggg gtgacgccca gcgcgccgtt gcgcagcgca tcgaggacgt tttcgacgcg
      781 gtgcgtgaag gcttcgatga aatggcattc gatgacgccg gcgccgccct tgatcgtgtg
      841 cgcggcgcgg aagatggcgt tgatcttgtc cgggtcgtcc ggattctgtt ccagaccgag
      901 caggcaggat tccatctcgg caagctgctc gcggctttcc tggacaaaga cgctggtgat
      961 ttcatccatg cgggaattcc tcgactcaaa cggcttcgtg ggccggaatg accaacgggt
     1021 cgcccaactc ggcggccagg tcgcagaagt cgatcagcga actgaccgtc tggctgtggg
     1081 cgacgatgcg cgcccgtttg ccgcagcgct gcgcttcctt cttcagcagc accagcaatt
     1141 gcaggccggc ggcgtcgatc tcggccactt gcgacaggtt cagttccagt tcggcggtcg
     1201 ccgccagcgc gtcgagcagt tgctgcttct gctcaagtgc gtggtagatg gtcagcggct
     1261 ggctgacgac gagagggtcc ttggcagaca tcgggtactc ctagaacagt tcgatgcgcg
     1321 agctgaccgg cgccacaccg gctgtttcat gcttcagcac gctttgatgc gtggtgcgct
     1381 ggctttccat cacgtagcgg ctgtacagcg cctgcaggtg ctcggcgatc ggttcgtact
     1441 tgaacgcggg atcatcgaag gcgcgcagac gatcggccag caggccggcg tggccgtcga
     1501 gacgggacaa ggcctgcatc acctgctcga tctgctgtcg gctgacatcc tggaactgga
     1561 cgctggcctg cgcttccatg aacatcgccg ccaattgctg gctgctgtcg c
//
LOCUS       DAOSDX010000746         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25478_length_1611_cov_3.131748, whole genome shotgun sequence.
ACCESSION   DAOSDX010000746 DAOSDX010000000
VERSION     DAOSDX010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25478_length_1611_cov_3.131748"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1611)
                     /locus_tag="PLN02_13875"
     CDS             complement(<1..>1611)
                     /locus_tag="PLN02_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI53464.1"
                     /translation="RQNGLQVESAASGFLMMVGMTSPGGQFDEVALNDYLARNVVPEL
                     RRIDGVGRVQLFGAEQAMRVWVDPDKLTAYGLTMQDLAQAIEQQNAQIAPGRIGDEPA
                     SPGQRLTVPLTVQGQLATPAQFAAIVLRAGADGSKVLLGDVARVELGAQSYAFANREN
                     GVPATSAAIQLAPGGNAVRTAAAVRERLAELTANLPPGMAYSVPFDTASFVKLSIEKV
                     IYTLLEAMLLVFLVMYLFLQNLRYTLIPAIVAPIALLGTFALMLVAGFSINSLTMFGM
                     VLAIGIIVDDAIVVVENVERLMASHGLSPREATVQAMREITGAVIGITLVLTAVFIPM
                     AFASGSVGVIYRQFALAMAVSILFSALLALSLTPALCATLLRPVAPDHQHKRGFFGAF
                     NRAFERFGAGYGARVQRLLGRSGRMMVVFAVIGALLAGAIWLLPSSFLPEEDQGYFMT
                     SIQLPTGATSERTLDVVKAFEAHVATRPALDVNLVVQGFSFAGSGPNAAMAFTMLKDW
                     DQRNGASAGEEAALAQQAMADSVEGMVM"
BASE COUNT          284 a          562 c          539 g          226 t
ORIGIN      
        1 ctcatcacca tgccctcgac gctgtcggcc atggcctgct gggcgagcgc cgcttcctcg
       61 ccggcgctgg cgccgttgcg ctgatcccag tccttgagca tggtgaaggc catcgccgcg
      121 ttggggccgg agccggcgaa gctgaagccc tgcaccacca ggttcacgtc cagcgccggg
      181 cgcgtcgcga catgcgcctc gaaggccttg accacgtcca gcgtccgttc gctggtggcg
      241 ccggtcggca actggatcga ggtcatgaag taaccctgat cctcctcggg caggaaggac
      301 gaaggcaaca gccagatggc gccggcaagc agcgcgccga tcaccgcaaa caccaccatc
      361 atccggccac tgcgcccgag caggcgctgc acccgtgcgc cgtagccggc gccgaagcgc
      421 tcgaaggcgc ggttgaaggc gccgaagaag ccgcgcttgt gctgatgatc gggcgcgacc
      481 gggcgcaaca gcgtggcgca cagcgccggc gtcaggctca aggcgagcag cgccgagaac
      541 aggatcgaca ccgccatcgc cagcgcgaac tggcggtaga tgacgccgac cgatccgctg
      601 gcgaaagcca tcgggatgaa cacggcggtc aacaccaggg tgatgccgat caccgcgccg
      661 gtgatttccc gcatcgcctg caccgtcgct tcccgtggcg agagtccgtg gctggccatc
      721 agccgctcga cgttctcgac gacgacgatg gcgtcgtcga cgatgatgcc gatggccagc
      781 accatgccga acatggtcag cgagttgatc gagaagccgg ccaccagcat cagcgcgaag
      841 gtgccgagca gtgcgatcgg cgcaacgatg gccggaatca gcgtgtagcg caggttctgc
      901 aggaacaggt acatcaccag gaaaaccagc agcatggctt cgagcagcgt gtagatgacc
      961 ttctcgatcg acaacttgac gaaggacgcg gtatcgaacg gcaccgaata cgccatgccc
     1021 ggcggcagat tcgccgtcag ctcggccagg cgctcacgca cggcggcggc cgtgcgcacc
     1081 gcgttgccgc ccggcgccag ctggatggcc gcactggtcg ccggcacgcc gttctcgcgg
     1141 ttggcgaagg cgtaggactg ggcgcccagc tcgacccgcg ccacatcacc gagcagcacc
     1201 ttcgacccat cggccccggc gcgcaggacg atggcggcaa actgcgccgg cgtcgccagt
     1261 tgcccctgga cggtcagcgg cacggtcagg cgctgcccgg gcgaggccgg ctcgtcgccg
     1321 atgcgacccg gcgcgatctg cgcgttctgc tgctcgatgg cttgcgccag gtcctgcatc
     1381 gtcaggccgt aggcggtgag cttgtccgga tcgacccaga cccgcatcgc ctgctcggcg
     1441 ccgaacaact gcacgcggcc gaccccgtcg atacggcgca gctccgggac gacgttgcgc
     1501 gccaggtagt cgttcagcgc gacttcgtcg aactggccgc cgggcgaagt catgccgacc
     1561 atcatcagga agccggacgc cgccgactcg acctgcaggc cgttctgcct g
//
LOCUS       DAOSDX010000747         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25513_length_1609_cov_2.994208, whole genome shotgun sequence.
ACCESSION   DAOSDX010000747 DAOSDX010000000
VERSION     DAOSDX010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25513_length_1609_cov_2.994208"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLN02_13880"
     CDS             <1..220
                     /locus_tag="PLN02_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53465.1"
                     /translation="TLSDWGFGSAGCIEIIGNYDDFKNAIAELSGLPTASSDSAIQAL
                     VAAGKLSVVIQGAKVPDIRNNQTRLVHD"
     gene            230..751
                     /locus_tag="PLN02_13885"
     CDS             230..751
                     /locus_tag="PLN02_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53466.1"
                     /translation="MKTFAAAITACLLAISAIPGDAWAQIQTCTQKLAPIADERLGQA
                     VATLLRQHPALVAQADDAPCRHAKETARAISRTVGRDHLRRLTGYELVKLINGESYFT
                     VERFRTSSPDELQKLASALDGVRGRKLRSEANTVYAYFLVDDSLIIMISSAAGGEANA
                     ALFAEIRRGFPTQ"
     gene            complement(770..>1609)
                     /locus_tag="PLN02_13890"
     CDS             complement(770..>1609)
                     /locus_tag="PLN02_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53467.1"
                     /translation="AIVRAIVVSGEHRMALTSSLAELVVLLVEPSQMQANLVTRMLEH
                     QGIRRVTAVTSARAALAALNDPDPGQVVISSLYLPDMPGTELVMAMRAEPMLETIPFI
                     LVSSETRPQLLEPIRQSGACAIVAKPFTEQQLARALYAAVDNLNPPEELDVADIEALR
                     VLLVDDSLASRHYLRRLLEELGISRISECVNGREAVEVLQENMFDLVITDYNMPEMDG
                     RELTEYIRTQSWQADVPVLMVTSEQNMGRLAAVERAGVSAICDKPFEASSVRTLINDA
                     LRS"
BASE COUNT          282 a          523 c          527 g          277 t
ORIGIN      
        1 cacgctgagc gactggggct tcggttccgc cggctgcatc gagatcatcg ggaactacga
       61 cgatttcaag aatgccatcg ccgaattgtc cggcctgccg accgcctctt ccgacagcgc
      121 catccaggcg ctggttgccg ccggcaagct gagcgtcgtc atccagggcg ccaaggtgcc
      181 ggacatccgg aacaaccaga cccgcctggt tcacgactga aaggccagca tgaaaacctt
      241 cgctgccgcg atcaccgcct gtctactggc gatttcagcg atccccggtg acgcctgggc
      301 ccagattcaa acatgtacgc agaaactggc gccgatcgcc gacgagcgtc tcggccaggc
      361 cgttgctacg ctgctgcgcc agcatcccgc cctcgtggca caggcggacg acgcgccctg
      421 ccggcacgcc aaggaaaccg cccgggcaat ctcgcggacg gtgggccgcg accacctcag
      481 gcgtctgacc ggctacgagc tggtcaagct gatcaacggc gaaagctatt tcacggtgga
      541 gcgtttccgg acctcgtcgc cagacgagct gcaaaagctt gccagcgcgc tggatggcgt
      601 gcgcggacga aaactgcgca gcgaagccaa cacggtctac gcgtatttcc tggtcgacga
      661 cagcctgatc atcatgattt cttccgccgc cgggggagag gccaacgcgg cgctgttcgc
      721 cgagatccgt cggggcttcc cgacgcagta gcgcgccgcc ccggccgttt caggagcgca
      781 gcgcgtcgtt gatcagggtg cgcacgctgg acgcctcgaa cggcttgtcg cagatggcgg
      841 aaacgccggc ccgctcgacc gcggccaggc gccccatgtt ctgctcgctg gtcaccatca
      901 gcaccggcac gtcggcctgc cagctttggg tgcggatgta ttcggtgagt tcgcggccgt
      961 ccatttccgg catgttgtag tcggtgatga cgaggtcgaa catgttctcc tgcaatacct
     1021 cgaccgcctc gcggccgtta acgcattcgc tgatgcgcga gatccccagt tcttctagca
     1081 agcgccgcag gtaatggcgg gaagccagac tgtcatcaac gagcagcacg cgcagggcct
     1141 cgatgtcggc gacgtcgagt tcttccggcg ggttgaggtt gtccacggca gcatacaggg
     1201 cgcgcgccag ttgctgctcg gtgaagggct tggcgacgat ggcgcaggcg ccggactggc
     1261 ggatcggttc gagcagttgc ggccgggttt cgctggagac caggatgaag ggaatggttt
     1321 ccagcatcgg ctcggcacgc atcgccatca ccagttcggt gcccggcatg tccggcaggt
     1381 agaggctgct gatcaccacc tgcccgggat cggggtcgtt cagcgcggca agcgccgcgc
     1441 gcgccgaggt cactgcggtc acccgccgga tgccctggtg ttcgagcatg cgcgtgacga
     1501 ggttggcctg catctgcgac ggctcgacca gcaggacgac gagttcggcc agcgaggaag
     1561 tcagggccat gcggtgctct ccggaaacga cgatggccct cactatagc
//
LOCUS       DAOSDX010000748         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25538_length_1608_cov_2.323245, whole genome shotgun sequence.
ACCESSION   DAOSDX010000748 DAOSDX010000000
VERSION     DAOSDX010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25538_length_1608_cov_2.323245"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1608)
                     /gene="pnp"
                     /locus_tag="PLN02_13895"
     CDS             complement(<1..>1608)
                     /gene="pnp"
                     /locus_tag="PLN02_13895"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOI53468.1"
                     /translation="IGASAALAISGIPFAGPIGAARVGYINGQYVLCPTLSQLKDSQL
                     DLVVAGTEAAVLMVESEADQLSEEVMLGAVVFGHTEMQKAINAINELVEEAGKPEWDW
                     QPPAKDEALVARLKDLVGAKLEEAYSITSKQARSQRVKEISAEAVAALCTGEEGAPDA
                     NTVGNLFFELEASIVRGRILSGAPRIDGRNTRTVRPITMRSGVLPRTHGSALFTRGET
                     QALAVATLGTNRDEQIIDALSGEYRDRFMLHYNMPPYATGECGRVGTPKRREIGHGRL
                     AKRALLAVLPKPEDFSYSMRVVSEITESNGSSSMASVCGGCLALMDAGVPLKAHVAGI
                     AMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTTTGITALQMDIKIQGITKEIMQVA
                     LAQAQEARLHILNLMQEAAAGPREEMSAYAPRLYTFKINPEKIRDVIGKGGAVIRSIT
                     EETGTTIDIQDDGTITIAATSGDAAAAARAKIDAITAEVEIGKIYEGTVLKILDFGAI
                     VSVLPGKDGLLHISQIANERVNKVEDYV"
BASE COUNT          261 a          478 c          549 g          320 t
ORIGIN      
        1 acgtagtctt cgaccttgtt gacgcgttcg ttggcgatct gcgagatgtg cagcaggccg
       61 tccttgcccg gcaggaccga aacgatcgca ccgaagtcga ggatcttgag cacggtgcct
      121 tcgtagatct tgccgatctc gacttccgcg gtgatcgcgt cgatcttggc gcgggccgca
      181 gcagcggcat cgcccgaagt ggcggcgatg gtgatcgtgc cgtcgtcctg gatgtcgatg
      241 gtggtgccgg tttcctcggt gatgctgcgg atcacggcgc cgcccttgcc gatcacgtca
      301 cggatcttct ccggattgat cttgaaggtg tacagacgcg gggcgtaggc cgacatttct
      361 tcacgcgggc cggcggcagc ttcctgcatc aggttgagga tgtgcagacg ggcttcctgg
      421 gcttgtgcca gggcaacctg catgatttcc ttggtgatgc cctggatctt gatgtccatc
      481 tgcagcgcgg tgatgccggt ggtggtgccg gccaccttga agtccatgtc gccgaggtga
      541 tcttcatcgc cgaggatgtc ggtgaggacg gcaaagcggt tgccttcctt gatcaggccc
      601 atggcgatac cagcaacgtg cgccttgagc ggcacaccag cgtccatcag tgccagacag
      661 ccgccgcaga ccgaagccat cgacgaggag ccgttcgatt cggtgatttc ggaaaccaca
      721 cgcatcgagt acgagaagtc ttccggcttc ggcagcacgg ccagcagcgc gcgcttggcc
      781 agacgaccgt gaccgatttc acgacgcttc ggcgtaccga cacgaccgca ttcgccggtg
      841 gcatacgggg gcatgttgta gtgcagcatg aagcggtcgc ggtattcgcc ggacagcgcg
      901 tcgatgatct gctcgtcacg attggtgccg agcgtggcga cagccagcgc ctgggtttcg
      961 ccacgggtga acagggccga accgtgggtg cgcggcagga cgccggaacg catggtgatc
     1021 ggccgcacgg tacgggtgtt gcgaccgtcg atgcgcggcg cgccggagag gatgcggcca
     1081 cggacgatgc tggcttcgag ttcgaagaac aggttgccga cggtgttggc atccggcgca
     1141 ccttcttcgc cggtgcacag ggcggcaacg gcttcagcgg agatttcctt gacgcgctgg
     1201 ctgcgggctt gcttggaggt gatgctgtag gcttcttcca gcttggcgcc gaccaggtcc
     1261 ttcaggcggg cgaccagggc ttcgtccttg gccggcggct gccagtccca ttccggcttg
     1321 ccggcttctt cgaccagttc gttgatggcg ttgatcgcct tctgcatctc ggtatggccg
     1381 aaaaccacgg cgccgagcat gacttcttcc gacagctggt cggcttccga ttcaaccatc
     1441 agcacggcgg cttcggtacc ggcgacgacc agatcaagct ggctgtcctt gagctgcgac
     1501 agcgtcgggc agagcacgta ctgaccgttg atgtagccga cgcgggcggc gccgatcggg
     1561 ccggcgaacg gaatgccgga gatcgccagg gcggcggaag cgccgatc
//
LOCUS       DAOSDX010000749         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25591_length_1605_cov_2.737419, whole genome shotgun sequence.
ACCESSION   DAOSDX010000749 DAOSDX010000000
VERSION     DAOSDX010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25591_length_1605_cov_2.737419"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PLN02_13900"
     CDS             <1..1141
                     /locus_tag="PLN02_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI53469.1"
                     /translation="ATLGSAGAGNPDPATGGGITTWEGKYVGKTVADAVDLYANGPFE
                     LLGRKHELVLGASQAKRVWTNRSYWAGSNNIADYYHWNGKLPEPDWANAGWQGRDRET
                     IRESGAYAAARLNLRDDLKLIAGSRVLNYENETMKKSGVIVPYLGAVYDLNDRYSVYA
                     SYTSIYQPQSAQDAQGKTLDPLEGLNYEAGIKGSFFNGRLNASLAYFILEQDNYAEAT
                     GGLTPSGGTAYRAISGVRTKGFEAEISGQLTPQWQVHAGFNHKISRQQGNKVATMSPE
                     NQFTLSTSVKPAFLPGLTLGGGARWQDKAWADVGHPTLGTVKHTMASYWLLDAMASYQ
                     IDKHLSVNLNVSNLLDKKYYTLFDWYSTYTWGEPRSAMLTLKYKF"
     gene            complement(1243..1497)
                     /locus_tag="PLN02_13905"
     CDS             complement(1243..1497)
                     /locus_tag="PLN02_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOI53470.1"
                     /translation="MDWIVSLVIGGIVGWLASIVMKTNAQMGIVANVLIGIFGSSLGY
                     WLAGLLGMAPTGGLLRFAIAIAGASLLIFILGKLGMFRKG"
BASE COUNT          337 a          563 c          485 g          220 t
ORIGIN      
        1 cgcgacgctc ggctcggccg gcgcgggcaa ccccgacccg gcgaccggcg gcggcatcac
       61 cacctgggaa ggcaagtacg tcggcaagac cgtcgccgac gccgtcgacc tctacgccaa
      121 cgggccgttc gaactgcttg ggcgcaagca cgaactggtg ctcggcgcca gccaggccaa
      181 acgggtgtgg accaaccgca gctactgggc cggcagcaac aacatcgccg attactacca
      241 ctggaacggc aagctgcccg agccggactg ggccaacgcc ggctggcaag gcagggatcg
      301 cgaaaccatc cgcgagagcg gcgcctacgc cgccgcccgc ctcaacctgc gcgacgacct
      361 caagctgatc gccggcagcc gcgtgctcaa ttacgagaac gagacgatga agaaatccgg
      421 cgtcatcgtt ccctatctcg gcgccgtcta cgacctcaac gaccgctatt cggtctacgc
      481 cagctatacc agcatctacc agccgcagag cgcgcaggac gcccagggca agacgctcga
      541 cccgctcgaa ggcctcaact acgaggccgg gatcaagggc agcttcttca acggtcgcct
      601 caacgccagc ctcgcctact tcatcctcga gcaggacaac tacgccgaag ccacgggcgg
      661 cctgacgccg agcggcggca ccgcctaccg ggcgatcagc ggcgtccgca ccaagggttt
      721 cgaggctgag atttccggcc agctgacgcc gcaatggcag gtgcacgccg gcttcaacca
      781 caagatctcg cgccagcagg gcaacaaggt ggcgaccatg tcgccggaga accagttcac
      841 gctgagcacc agcgtcaagc cggccttcct ccccgggctc acgctcggcg gcggcgcccg
      901 ctggcaggac aaggcctggg ccgacgtcgg ccatccgacg ctgggcacgg tcaagcacac
      961 catggccagc tactggctgc tcgacgcgat ggccagctac cagatcgaca agcacctgtc
     1021 ggtcaacctc aacgtcagca acctgctcga caagaagtac tacacgctgt tcgactggta
     1081 cagcacctac acctggggcg aaccgcgcag cgcgatgctg acgctgaagt acaagttctg
     1141 acccggaccg ggccacaagc aaaaacggcg cccctgccag ggcgccgttt ttcttgccgg
     1201 ttgacggtcg accgtcgaat ccgggcaatt tcccgccggc atctagccct tgcggaacat
     1261 ccccagcttg ccgaggatga agatcagcag cgaggcaccg gcaatggcga tggcgaagcg
     1321 cagcaggccg ccggtcggcg ccatgccgag caggccggcg agccagtagc cgagcgacga
     1381 accgaagatg ccgatcagca cgttggcgac gatccccatc tgggcgttgg tcttcatgac
     1441 gatgctggcc agccagccga cgatgccgcc gatcaccagg gaaacgatcc agtccatctg
     1501 cgtactcctt tccagagagc gtgggtgaag catgacgata acgcatcccg gcgatcgccg
     1561 ggtctgcccc ggcgccaaac atttttgtaa cggcgtgtaa ccaaa
//
LOCUS       DAOSDX010000750         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25610_length_1604_cov_3.779858, whole genome shotgun sequence.
ACCESSION   DAOSDX010000750 DAOSDX010000000
VERSION     DAOSDX010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25610_length_1604_cov_3.779858"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLN02_13910"
     CDS             <1..371
                     /locus_tag="PLN02_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI53471.1"
                     /translation="ERGLEFSFAGGGKLQFRGEAQDLEEMLGNLLDNAGKWASRRVEV
                     TCASDGATLSIDIDDDGPGIAAADRALLLQRGQRGDERVPGSGLGLAIVDELAGLYGG
                     RLELLDSPLGGLRARLSLAA"
     gene            complement(368..853)
                     /locus_tag="PLN02_13915"
     CDS             complement(368..853)
                     /locus_tag="PLN02_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOI53472.1"
                     /translation="MISRRRFIALAPAFWVGSSFAQKVPMPGKMDLCPVCGMVVSKYP
                     NWIAVVTWKDGHAHFFDGAKDMFKFLFDLPKYAPGHRTQEITGIHVTDFYNLERIDGR
                     RALYVIGSDVYGPMGHEFVPLATAEDAADFLKEHKGKKILRFDQVTAKLPFRLDDGKF
                     D"
     gene            complement(850..1458)
                     /locus_tag="PLN02_13920"
     CDS             complement(850..1458)
                     /locus_tag="PLN02_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOI53473.1"
                     /translation="MKRREMLKLSALAGLASAALPSVAHAESCQTDGTPNQFIPKKAA
                     DANPLDKDIEKYPKCPYCGMDRKEHNKTRMLVQYSDDLADGVCSIHCLALSLSLNIDR
                     EPKEIWGPDFAASAEPRPLLPVDKLTYLLGADLKHAMTRKSKHSFASADVAREFQAKH
                     GGTLVSFNDALRESYLGMADDVMMIRKNRAERRRRAMEKQQG"
BASE COUNT          272 a          490 c          530 g          312 t
ORIGIN      
        1 cggaacgagg gctcgaattt tcattcgctg gcggcggcaa gctgcagttc cgcggcgaag
       61 cccaggatct cgaggaaatg ctcggcaacc tgctcgacaa tgccggcaag tgggccagtc
      121 gccgggtcga ggtaacttgt gccagcgacg gggcaacgct gtccatcgac atcgacgacg
      181 acggcccggg catcgccgcc gccgaccggg cgctgcttct gcaacgcggc caacgcggtg
      241 acgaaagagt gccgggcagc ggtctcggac tggcgatcgt cgacgaactg gccggcctct
      301 acggcggccg cctcgaactg ctcgattcgc cgctcggcgg cctcagggca cgcctcagcc
      361 tggcggctta gtcgaacttg ccgtcgtcga gccggaacgg cagtttcgcg gtgacctggt
      421 cgaaacgcag gatcttcttc cccttgtgtt ccttgaggaa gtcggcggcg tcttccgccg
      481 tcgccagcgg cacgaactcg tgccccatcg gcccgtaaac atccgaaccg atgacgtaga
      541 gcgcccggcg gccgtcgatc cgttccagat tatagaaatc ggtgacgtgg atgccggtga
      601 tctcctgcgt ccggtggccg ggcgcgtact tgggcaggtc gaacaggaac ttgaacatgt
      661 ccttggcgcc gtcgaagaaa tgggcgtgcc catccttcca ggtgacgacg gcgatccagt
      721 tcgggtactt cgatacgacc atgccgcaca ccgggcagag gtccatcttg cccggcatcg
      781 gcaccttctg ggcgaagctg ctgcccaccc agaaggctgg tgccagcgcg atgaaacggc
      841 gccggctgat catccctgct gtttctccat ggcgcggcgg cgacgttcgg cgcggttctt
      901 gcggatcatc atgacgtcgt cggccatgcc gagatagctc tcacgcagcg cgtcgttgaa
      961 gctgaccagc gtaccgccgt gtttggcctg gaattccctg gcgacatcgg cggaagcgaa
     1021 ggaatgcttg ctcttcctgg tcatcgcgtg cttgaggtcg gcaccgagca gataggtcag
     1081 cttgtcgacc ggcagcagcg gccgcggctc ggcgctggcg gcgaagtccg ggccccagat
     1141 ttccttcggt tcgcggtcga tgttcaggct caggctgagc gccagacagt ggatcgagca
     1201 gacgccgtcg gccaggtcgt cggaatactg gaccagcatc cgggtcttgt tgtgttcctt
     1261 gcggtccatg ccgcagtacg ggcacttcgg gtacttctcg atgtccttgt cgagcgggtt
     1321 ggcatcggcc gcctttttcg ggatgaactg gttgggcgtg ccgtcggtct ggcagctttc
     1381 ggcatgcgcc accgaaggca gggcggccga ggcaaggccg gccagcgcgg acagtttgag
     1441 catttcacga cgtttcatct tggctcctcg agggacggta aataagcttt gcatgttaat
     1501 ggtccgccgt gcgcgcaagc tgcggcatat tgccgcacca gggaactttc aggattcgat
     1561 caggatcagc ttcgagttga cgacaaacca tgtggcaagc agcg
//
LOCUS       DAOSDX010000751         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25657_length_1602_cov_2.527473, whole genome shotgun sequence.
ACCESSION   DAOSDX010000751 DAOSDX010000000
VERSION     DAOSDX010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25657_length_1602_cov_2.527473"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PLN02_13925"
     CDS             <1..1005
                     /locus_tag="PLN02_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOI53474.1"
                     /translation="KNATSYARALDGDFAKGALSTSTVGTEKHTSPFLHYEFSPLDSL
                     RLSMGIRKDDFDLETDNKLGDKGDGRKSFSKLVRKFGATYEFAGGYLLWARAAEGFLA
                     PSVSDMLGSGGTPNQTTYVPMNMSLKPIESMTYELGLRGQLLAKRLRFDTAYFDTDLK
                     NMIVREDCAPSEPCYQRNVNAGKARLHGLETSIVFDVTRWLDTGLSHTWSIVEYKDYK
                     TPTYDYSGNTWKGAPAHHLNLRVGVKPVEGLRAELEYDHIASYYRNNENVGGTYKRPD
                     LFHLRIAYKTKTWSAWLHVLNLFDKKYAERAWMTAAGKATYDEGYKPLTLRAGVSYNF
                     "
     gene            1018..>1602
                     /locus_tag="PLN02_13930"
     CDS             1018..>1602
                     /locus_tag="PLN02_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53475.1"
                     /translation="MRMVKELVLLSMLLCAAQVPAQETGERRGPPPGVSRGDYAGPRP
                     PADASDEEKRAFWRARAAERRQAETAPLAAEAAAPAAEKAPPAADARPGKPAAGQAPA
                     ARAQAVDPHAGHTMPMAAPPAPAAAASGRPRGMRGGGDGVLWLSDQAPRRDGGGRPGG
                     ARGGNPMAAMGGAGGMDDMGMGGAPGRVPNKRLWL"
BASE COUNT          298 a          540 c          538 g          226 t
ORIGIN      
        1 aagaacgcga cttcctatgc ccgcgcgctg gacggcgatt ttgccaaggg ggcgctgagc
       61 acctctaccg tgggcacgga aaagcacacc tcgcccttcc tgcattacga gttctcgccg
      121 ctcgattcgc tccgcctgtc gatgggaatc cgcaaggacg acttcgatct cgagacggac
      181 aacaagctgg gcgacaaggg cgacggccgc aagagctttt ccaagctggt ccgcaagttc
      241 ggtgccacct acgaattcgc tggcgggtat ctgctctggg cgcgcgccgc cgaaggcttt
      301 ctggcgccga gcgtgagcga catgctgggc agcggcggca ccccgaatca aaccacctat
      361 gtgccgatga acatgagcct caagccgatc gaatcgatga cctacgagct gggcttgcgc
      421 ggccagttgc tggcgaagcg cctgcgcttc gatacggcgt atttcgatac cgacctgaaa
      481 aacatgatcg tccgcgaaga ctgcgcgccg agcgagccgt gctaccaaag gaacgtcaac
      541 gccggcaagg cccgcctgca cggcctggaa acctccatcg tgttcgacgt cacgcgctgg
      601 ctggataccg ggctatctca tacctggagc atcgtcgagt acaaggatta caagacaccg
      661 acctacgact actccgggaa tacctggaaa ggcgcgccgg cgcaccactt gaacctgcgc
      721 gtcggggtca agcccgtcga aggtttgcgc gcggagctgg agtacgacca tatcgcctcc
      781 tactaccgca acaacgagaa cgtcggtggc acctacaagc gaccggacct attccacctg
      841 cgcatcgcct acaagaccaa gacctggagc gcgtggttgc acgtcctcaa cctgttcgac
      901 aagaaatacg ccgagcgcgc ctggatgacc gccgccggca aggcgactta cgacgaaggc
      961 tacaaaccgc tgaccctgcg cgccggcgtc agctacaact tctgacggga gcgaaacgtg
     1021 cgcatggtga aagaactggt tctgttgtcg atgctgctct gcgcggcaca agtgccggcc
     1081 caggagaccg gcgagcggcg cgggccgccg cccggcgtca gtcgcggcga ttacgccggg
     1141 ccgcggccgc cggccgatgc ttccgacgaa gaaaagcggg ccttctggcg ggcgcgagcg
     1201 gccgagcggc gccaggccga gaccgcgccg ctagctgcag aagccgctgc gccggcagcc
     1261 gaaaaggcgc cgccggcggc tgatgctcgc ccaggcaagc cggcagctgg tcaggcaccg
     1321 gccgctcgcg cccaggcggt cgatccgcat gccgggcaca cgatgccgat ggccgcaccg
     1381 ccggcgccgg ccgccgccgc ctcgggccgg ccgcgcggca tgcgcggcgg cggcgacggc
     1441 gtgctctggt tgagcgacca ggcgccgcgc cgcgacggcg gcgggcgtcc gggtggcgcc
     1501 cgcggaggca atccgatggc ggctatggga ggcgcgggcg gcatggacga catgggcatg
     1561 ggcggcgcgc ccggccgcgt cccgaacaag cgcctgtggc tg
//
LOCUS       DAOSDX010000752         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25661_length_1602_cov_2.278604, whole genome shotgun sequence.
ACCESSION   DAOSDX010000752 DAOSDX010000000
VERSION     DAOSDX010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25661_length_1602_cov_2.278604"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLN02_13935"
     CDS             <1..460
                     /locus_tag="PLN02_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53476.1"
                     /translation="EVAVAIRALLDENFLGAYHLDWATSQAEAMTILERGKVSIIVSE
                     TRVDGEDVTPFLKTLKHFHAQIVTIVLTSFSDTSLLTDLINQGQIHRFLPKPMRKTLT
                     VRSIRSGIDRHREIVQRPQLSLRHRVEPPRATVNHSMISRIRGIFHRVSI"
     gene            complement(661..1515)
                     /locus_tag="PLN02_13940"
     CDS             complement(661..1515)
                     /locus_tag="PLN02_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOI53477.1"
                     /translation="MSQFSFDVALEDFEAKVLQASLEVPVVVDFWAPWCGPCQTLKPM
                     LEKLAEEYKGRFLLAKVNSDENPELAGHFGVRSIPSIKVVYQGQLVDEFNGALPEGKV
                     REFLDRIALPGGNETDLRAEAAALVGEGRLEEALARLVAASQGDPQDQAVQLDAIDVL
                     LQLGRNDEAAQLLAGDYPNEPDRATALRARLALAQGAADSAPLEEKLAANPDDHATRL
                     ELARAYAAQHRFREALEAALEVVRRDRFFDEGAGRKTLLQFFEALSGEQYDDLVREFR
                     RKLSAALN"
BASE COUNT          261 a          492 c          534 g          315 t
ORIGIN      
        1 ggaagttgcc gttgccatcc gtgccctgct cgacgaaaat ttcctcggtg cctatcacct
       61 cgactgggct acttcgcagg ccgaggccat gacgatcctc gaacggggca aggtatcgat
      121 catcgtctcg gaaacccgtg tcgatggtga ggatgtcact cccttcctga aaaccctgaa
      181 gcactttcat gcccagatcg tgaccatcgt cctgacttcg ttcagcgaca cctcgctgct
      241 caccgatctg atcaaccagg ggcagatcca ccgcttcctg cccaaaccca tgcgcaagac
      301 gttgaccgtc cgcagcattc ggtcgggcat cgatcgtcac cgggaaatcg tgcaacgtcc
      361 gcagctttca ctgcgccacc gcgtcgagcc gccgcgcgct accgtcaatc actcgatgat
      421 cagtcgcatc cggggaatat tccaccgcgt ttcaatctga ctctgtccgg gattccgtcg
      481 gccatcgggg tacgacatgc gagctgtctt tgctgcagat gctgttggtc ctgaagccct
      541 gatctgcaac tgcggaaaac cgaagtcttt ctgtgccggc cggttcggcg agccgccccg
      601 gggcggaccc ggcggcggct ggatgaaggg gaagggctgc acgacatgca gcccgggggg
      661 tcagttgagg gccgccgaca gcttgcggcg gaactcgcgg acgaggtcgt cgtattgttc
      721 gccggacagc gcctcgaaga attgcagcag tgtcttgcgg ccggcgccct cgtcgaagaa
      781 gcggtcgcgg cggaccacct ccagtgccgc ttcgagcgct tcgcggaaac ggtgctgggc
      841 agcgtaggcg cgggccagtt cgaggcgggt ggcgtgatcg tccgggttgg cggcgagctt
      901 ttcttcgagc ggtgcgctgt cggcggcgcc ctgggcaagc gccaggcggg cgcgcagggc
      961 ggtggcgcga tccggttcgt tcgggtagtc gccggccagc aactgggcgg cttcgtcgtt
     1021 gcggccgagt tgcagcagga cgtcgatggc gtcgagttgc accgcctggt cctgcgggtc
     1081 gccctggctg gcggcgacca gtctggccag cgcttcttcc agtcggcctt cgccgaccag
     1141 ggcggcggct tctgcgcgca ggtcggtctc gttgccaccg ggtagggcga tgcggtcgag
     1201 gaattcgcgg accttgccct cgggcagggc gccgttgaat tcgtcgacca gctgaccctg
     1261 gtagacgacc ttgatcgacg gaatgctgcg cacgccgaag tggccggcca gttccgggtt
     1321 ctcgtcggag ttgaccttgg ccagcaggaa acggcccttg tattcttcgg ccagcttttc
     1381 cagcatcggt ttcagcgtct ggcagggacc gcaccagggg gcccagaagt cgacgaccac
     1441 cggtacttca agcgatgcct ggaggacctt ggcttcgaaa tcttcgaggg cgacgtcaaa
     1501 ggaaaattgg gacatgagca gacttccggg tggtttttca ggaagcgaca attctagccg
     1561 agtcgctttc gcggtggcag acggctttcg ctgccggggg at
//
LOCUS       DAOSDX010000753         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25701_length_1600_cov_2.911974, whole genome shotgun sequence.
ACCESSION   DAOSDX010000753 DAOSDX010000000
VERSION     DAOSDX010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25701_length_1600_cov_2.911974"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PLN02_13945"
     CDS             complement(<1..1251)
                     /locus_tag="PLN02_13945"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HOI53478.1"
                     /translation="MKLLHELATQLLLGSERRPPTLPETPGEFGAFLQALALPGDDRE
                     IGLLRQAGSLAVCAAAGYAPAAAATPPLPTCPPEQTAVADDRQLVATLQQILADGPDA
                     LRREAFTRLASRATCLAPELLVPALTLGQKNRDLRPSLLAVLGERGRWLAALNPDWSY
                     AAGSAEAAADPSLWEEGSQEQRKQLLTRLRQSDPDRARCLLQEGFAQLEARERASLLE
                     TMGYGLGPSDEDFLENLLVDRSKEVRQRAASLLAALPDSRYSQRMAQRMAACLGAERK
                     LFRTVRTLEAPAAFAADWKNDALEESRPKSESLGERAWWLYQIARGLPLDWWEKQTGL
                     SPAELIKWVRATDWSEAILRAWHEALLRQPAAAWAGEFLTHGGSWGIEIDVFALLAFL
                     PAREREQYWLSLLKEQSPGHTRGWV"
     gene            complement(1248..>1600)
                     /locus_tag="PLN02_13950"
     CDS             complement(1248..>1600)
                     /locus_tag="PLN02_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53479.1"
                     /translation="LSTGDTQLLPAAHPPSVALDGALSGLAEAIAANPWQSPQPMLIE
                     GGIPQPDAAGWLLRCPENRQLRLQVRDEDGWTLLAHSGGKPLTVFGEWHGDVLHPLAA
                     WQGKPIWQKGMEEA"
BASE COUNT          223 a          541 c          567 g          269 t
ORIGIN      
        1 gacccagccg cgcgtatgac ccggcgactg ctctttcagc aagctgagcc agtattgttc
       61 gcgctcgcgg gcgggcagga aggcgagcag cgcgaagacg tcgatctcga ttccccacga
      121 cccgccgtga gtcaagaact cgccggccca ggcggctgcc ggctggcgca gcagggcctc
      181 gtgccaggcg cgcaggatgg cttcgctcca gtccgtcgcg cgcacccact tgatcagttc
      241 ggccgggctc aggccggtct gtttctccca ccagtccagc ggcaggccgc gcgcgatctg
      301 gtacagccac caggcgcgct cgcccaggct ttcgctcttc ggccggcttt cttccagcgc
      361 gtcgttcttc cagtcggcag cgaaagcggc cggcgcttcc agggtgcgca cggtgcgaaa
      421 gagcttgcgc tccgcgccaa ggcaggccgc catccgctgt gccatgcgct ggctgtaacg
      481 actgtcgggc aaggcggcga gcaggctggc ggcgcgttgg cggacttcct tgctgcggtc
      541 gaccagcaaa ttttcaagaa aatcctcgtc cgacggtccc agtccgtacc ccatcgtttc
      601 cagcaggctg gcccgctcgc gcgcttcgag ttgcgcgaaa ccctcctgca gcaggcaacg
      661 cgcccgatcg ggatcgcttt gccgcaagcg ggtaagtagc tgcttgcgct gttcctggct
      721 accttcttcc cacaggctcg ggtcggcggc cgcttcggcg ctgccggcgg cgtaggacca
      781 gtccgggttg agcgcggcca gccagcggcc gcgctcgccg aggacagcga ggagggaagg
      841 gcgcagatcg cggtttttct ggcccagcgt cagcgccggc acgagcagtt ccggcgccag
      901 acaagttgcc cggcttgcca gccgggtaaa ggcttcgcgg cgcaaggcgt cggggccgtc
      961 ggcaagaatc tgttgcaggg tcgccacgag ttgccggtcg tcggccactg ccgtctgttc
     1021 cggcgggcat gtcgggagcg gcggcgttgc ggcggcggcc ggggcgtagc cggccgccgc
     1081 gcagacggcc aggctgcccg cctggcgcag caggccgatt tccctgtcgt cgcccggtag
     1141 ggcgagggcc tggagaaaag cgccgaattc gcccggcgtt tccggcaggg tcggcgggcg
     1201 gcgttcgctg ccgagcagca gttgagtggc cagttcgtgc agcaacttca tgcttcttcc
     1261 atccccttct gccagatcgg cttgccctgc caggcggcga gcggatgcag gacgtcgccg
     1321 tgccattcgc cgaatacggt gagcggctta ccgccgctgt gtgccagtag cgtccagccg
     1381 tcctcgtcgc gcacttgcag ccggagttgc cggttttccg ggcagcgcag caaccagccg
     1441 gcggcgtcgg gctggggaat accgccctcg atcaacatcg gttgcggcga ttgccacggg
     1501 ttggcggcga tggcttcggc cagcccggac agcgcaccgt cgagggcgac gctcggcgga
     1561 tgggcggcgg gcagcaattg ggtatcgccg gtgctcagcg
//
LOCUS       DAOSDX010000754         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25772_length_1596_cov_4.386113, whole genome shotgun sequence.
ACCESSION   DAOSDX010000754 DAOSDX010000000
VERSION     DAOSDX010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25772_length_1596_cov_4.386113"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="PLN02_13955"
     CDS             complement(<1..1565)
                     /locus_tag="PLN02_13955"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOI53480.1"
                     /translation="MFAGVPIVFAGINNDPRQLIGERAGVTGVAERFDLTRTLQLAQR
                     LHPEVKRLVFLTAADESGAGMRKHIAEALANLGTEINVEHWITQNLAVADGRLPLLGA
                     DSLLFALGPQPQIAGGRPLGPEDLVAHVHSRTSRPVYSDLDATVGRGALGGYMNSGLE
                     NGRLLARMAQRVLAGERPEDIPIVYETPQALIFDYRELQRFGITESELPPGSQLRHRP
                     PSIFDPEYRSALLGFTAIVGALLLVLAGVLIRGRILASRHAALHYQATHDDLTGLPNR
                     AWLNEQLLHGKWGAGRSVALVMADINRFQLINDTYGHSFGDEVVSCVAERLAGIQDGQ
                     TQLIRFSGDAFVILRRLTDAQQLETLCAQCQRILSEPFHVAGHRLSVSAAFGVTLGAP
                     GEIDRDRLLREADTAMHEAKRERGNQVVRFDRDIHERALRQFRLEAWLPNAVANGEIQ
                     VFFQPIVENGNGSIAGFEALARWQHPELGWVPPPEFIRAAIETGCIRDLTLGMLRAAS
                     RAFMPYLEAASRP"
BASE COUNT          265 a          475 c          586 g          270 t
ORIGIN      
        1 tagggacggg aagccgcttc cagatagggc atgaaggccc ggctggcggc acgcagcatg
       61 cccagcgtca ggtcgcggat gcagccggtt tcgatggcgg cgcgaatgaa ttcgggcggc
      121 ggcacccagc ccagctcggg gtgctgccag cgcgccaggg cctcgaagcc ggcgatgctg
      181 ccattaccgt tttcgacgat gggctggaag aagacctgga tctcgccgtt ggcgaccgca
      241 ttcggcagcc aggcctcaag gcggaactgg cgcagcgcac gttcgtggat gtcgcggtcg
      301 aagcgtacca cttggttgcc gcgctcgcgc ttggcttcgt gcatcgccgt gtccgcctcg
      361 cgcaacaggc ggtcgcggtc gatttcgccg ggggcgccca gggtcacgcc gaaggccgcc
      421 gataccgaaa ggcggtgccc ggcaacgtga aacggctcgc tcaggatacg ctggcattgg
      481 gcgcacaggg tttccagttg ctgcgcatcg gtgagtcggc gcaggatgac gaaagcgtcg
      541 ccgctgaagc ggatgagctg ggtctgacca tcctggatgc cggccaggcg ctcggcgacg
      601 caggagacga cctcgtcgcc gaaggagtgc ccgtaagtgt cgttgatcaa ctggaagcgg
      661 ttgatgtccg ccatgaccag ggcgacgcta cgcccggccc cccatttccc gtgcaggagc
      721 tgttcgttga gccaggcacg gttgggcaga ccggtcaggt cgtcgtgggt ggcctggtag
      781 tgcagggcgg catggcgcga ggcgagaatg cggccacgga tcagcacgcc ggcgagcacc
      841 aggagcagcg cgccgacgat ggcggtgaag ccgagcaggg cgctgcggta ttccgggtcg
      901 aagatcgatg gtggccggtg gcgcagttgg ctgcccggtg gcagctcgct ttcggtaata
      961 ccgaaacgct gcagttcgcg gtagtcgaaa atcagggctt gcggcgtttc atagacgatc
     1021 gggatgtctt ccgggcgttc gccggcgagc acgcgctgcg ccatccgtgc cagcaggcga
     1081 ccgttctcca ggccgctgtt catgtagccg cccagggcgc cgcgcccgac ggtggcgtcg
     1141 aggtcggaat agaccgggcg gctggtccgc gaatggacat gggcgaccag gtcctcgggg
     1201 cccaaggggc gtccgccggc aatctggggt tggggcccaa gggcgaacaa caagctatca
     1261 gcaccgagca gcggcaggcg tccgtcggct acggccagat tctgtgttat ccagtgctcg
     1321 acgttgattt ccgtgcccag attcgccagt gcctcggcga tatgttttcg catgccagca
     1381 ccggattcgt cggcggcagt caggaatacc agccgtttga cttcggggtg caggcgctgg
     1441 gccagttgca gggtgcgtgt gagatcgaaa cgttcggcga cgccggtgac gccggcgcgt
     1501 tcgccgatga gttggcgcgg gtcgttgttg atgccggcaa agacgatagg tacgccggca
     1561 aacatcgcgt gcccgacggc gaactccagc gcgttg
//
LOCUS       DAOSDX010000755         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25776_length_1596_cov_2.539260, whole genome shotgun sequence.
ACCESSION   DAOSDX010000755 DAOSDX010000000
VERSION     DAOSDX010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25776_length_1596_cov_2.539260"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLN02_13960"
     CDS             complement(<1..201)
                     /locus_tag="PLN02_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53481.1"
                     /translation="MKILIVDDEPAIADTLSYALRAEGFATECCTLGGEALMRLQQGG
                     VDFVVLDVGLPDITGFDVCRALR"
     gene            420..1193
                     /locus_tag="PLN02_13965"
     CDS             420..1193
                     /locus_tag="PLN02_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI53482.1"
                     /translation="MAVELFNDGKHIVHAFYDLVDDSPGGAVQCNQFLIVDNGHGALI
                     DPGGNMTYSGLLMDMQKYFSSKDLDYILASHPDPDIIASVNKWFVASHCKVMISSLWT
                     RFVPHFTTGKDVSHRIVGIPDQGSVIRLGQSNVLALPAHFMHAEGNFQFYDPVAKILF
                     TGDLGASLVPQALAASAVTDFHSHVGYMEGFHKRYIVSRKVCRFWANMVRQLEIEQIV
                     PQHGSRFVGRRAVEDFISWVESLECGVDLMTQGNYQIPR"
     gene            complement(1201..>1596)
                     /locus_tag="PLN02_13970"
     CDS             complement(1201..>1596)
                     /locus_tag="PLN02_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HOI53483.1"
                     /translation="FCAAWKKHLERLPVVGAEIAAADIAARHPEYHALLASPEMALNQ
                     DWTPDGGQMNPFLHLSLHLAIHEQLSIDQPPGIRAAFDSLRARMDAHEAEHVLLECLG
                     ETIWRAQRQGTAMDAQAYIEAIRLRAGQR"
BASE COUNT          271 a          481 c          510 g          334 t
ORIGIN      
        1 gcgcagcgcc cggcagacgt cgaaacctgt gatgtcgggc aggccgacgt cgaggacgac
       61 gaaatcgacg ccgccttgtt gcagccgcat cagcgcctcg ccgcccagcg tgcagcattc
      121 ggtggcaaag ccctcggcgc gcagcgcgta gctcagcgta tcggcgatgg ccggttcgtc
      181 gtcgacgatc aggatcttca tgatgccggc aatgataaga catcgcgcgc gccgtcaact
      241 tgccgtgttg gtgatttacg cgattggcgg tgagcttgtg cgcaacatta agctattggt
      301 atggtgagcg tatccttcgc cgttctcggt tctggcgcgc cgggcaagcc cccagcatag
      361 gccggactag gcagaagcgg atggcagggg gataattggc cattcggtat gggggtgata
      421 tggcagttga attgtttaat gacggcaagc acatcgtcca cgcgttctac gatctggtag
      481 acgattcgcc aggcggcgcg gttcagtgca accagttcct gatcgtcgac aacggtcacg
      541 gcgcgctgat cgatcccggc ggcaacatga cctactccgg gctcctgatg gacatgcaga
      601 agtatttctc gtccaaggat ctggattaca tcctggcatc ccacccggac ccggacatca
      661 tcgcttcggt caacaagtgg ttcgtcgcct cgcactgcaa ggtgatgatt tcctcgttgt
      721 ggacgcgttt cgtcccgcac ttcacgaccg gcaaggacgt ttcccaccgc atcgtcggca
      781 ttcccgatca gggatcagtg atccgccttg gccagagcaa tgttctggcg ctaccggcgc
      841 atttcatgca tgccgaaggc aatttccagt tctacgaccc ggtggccaag atcctgttta
      901 ccggcgacct cggtgcctca ctggtgccgc aggcgttggc ggccagtgcg gtgaccgatt
      961 tccatagcca tgtcggctac atggagggct tccacaagcg ctacatcgtt tcgcgcaagg
     1021 tctgccgctt ctgggccaac atggttcgcc agctcgagat cgagcagatc gtgccgcagc
     1081 acggctcgcg tttcgttggc cggcgcgccg tcgaggattt catcagttgg gtcgaaagcc
     1141 tggagtgcgg ggtggacctg atgacccagg gcaactacca gattccccgc tgattccttc
     1201 tcagcgctgg ccggcccgca gccggatggc ctcgatgtag gcctgcgcgt ccatcgccgt
     1261 tccctggcgc tgggcgcgcc agatggtttc tcccagacat tccagcaaca cgtgttcggc
     1321 ctcgtgcgcg tccatccgtg cgcgcaggct gtcgaatgcc gccctgatcc ccggcggctg
     1381 gtcgatgctc aactgctcat gaatcgccag gtgcagcgag aggtggagga aggggttcat
     1441 ttgcccgccg tccggcgtcc agtcctggtt gagtgccatt tcggggctgg cgaggagggc
     1501 gtggtactcg gggtgccggg cggcaatgtc ggcggcggcg atttcggcgc cgaccaccgg
     1561 caggcgttcg aggtgttttt tccaggcggc gcagaa
//
LOCUS       DAOSDX010000756         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25801_length_1595_cov_2.373377, whole genome shotgun sequence.
ACCESSION   DAOSDX010000756 DAOSDX010000000
VERSION     DAOSDX010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25801_length_1595_cov_2.373377"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLN02_13975"
     CDS             complement(<1..215)
                     /locus_tag="PLN02_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53484.1"
                     /translation="MDLAQITESLQRDAVWVVFLNVLAQQGGLPVPAVPTLLLAGSLA
                     LSSGQLAAMLAAAILASVLADALWYLAG"
     gene            complement(276..1157)
                     /locus_tag="PLN02_13980"
     CDS             complement(276..1157)
                     /locus_tag="PLN02_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOI53485.1"
                     /translation="MTATLSTVLQSRRVERLVQGKPTSDGAGVRLTRVLTQNLQRRLD
                     PFLMLDAFRNENPEDYIGGFPDHPHRGFEAVTYMLAGRMRHHDTAGNAGLLGPGGAQW
                     MTAGSGLIHSELPEQEEGLMEGFQLWLNLPARDKMIAPSYRDIPPEQIPEATTAAGVR
                     VRIIAGSSQGVAGAVQREATQPLYLDVHLPAGGRFEQAIPAGHNAFVYVYRGSVEVGG
                     TVVEDRHMAILANDGGDGVVLAAAEDARALIIAGQPLGEPIAQYGPFVMNTGAEIEQT
                     LRDYRDGKFEAAVVQSL"
     gene            complement(1206..>1595)
                     /locus_tag="PLN02_13985"
     CDS             complement(1206..>1595)
                     /locus_tag="PLN02_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOI53486.1"
                     /translation="ARVALDDALIAQVQAVDVIVLGVPMYNFGVPVQLKTWIDAIARA
                     GVTFRYTANGPEGLLKGKKVYVALARGGLYRDTPADSQVPYLKSVLAFLGMTDVEFIY
                     AEGLAMGADSASKAFAEAAARIEEVVV"
BASE COUNT          249 a          525 c          570 g          251 t
ORIGIN      
        1 ccggccaggt accagagcgc atcggccagc accgaggcaa ggatggcggc ggcgagcatg
       61 gccgccagtt gcccggacga cagcgccagg ctgccggcga gcagcagcgt cggcaccgcc
      121 ggcaccggca ggccgccctg ctgcgcgagg acgttgagaa aaaccaccca gacggcgtcg
      181 cgctggagcg attcggtaat ctgggcgagg tccatgcggg cgggctttca ggaagacgcg
      241 ggaaccggct tacgaccccg gccccgccgg atggttcaca aggattgcac caccgccgcc
      301 tcgaacttgc cgtcgcggta gtcgcgcaag gtctgttcga tctcggcgcc ggtgttcatc
      361 acgaacgggc cgtactgggc gatgggctcg cccagcggct ggccggcgat gatcagggcg
      421 cgggcatctt cggcggcggc gagcaccacg ccgtcgccac cgtcgttggc gaggatagcc
      481 atgtggcgat cctccaccac cgtgccgccg acctcgacgc tgccgcgata gacatagacg
      541 aaggcgttgt gcccggccgg gatggcctgt tcaaaacgcc cgccggccgg cagatgcacg
      601 tccaggtaga gcggttgcgt cgcctcgcgc tgcacggcac cggccacgcc ctggctgctg
      661 ccggcgatga tgcgcacgcg cacgcccgcc gccgtggtcg cctcgggaat ctgttccggc
      721 gggatgtcgc ggtagctggg ggcgatcatc ttgtcgcgcg ctggcaggtt gagccagagc
      781 tgaaaacctt ccatcagccc ttcttcctgt tccggcagtt cggaatgaat gaggccgctg
      841 cccgccgtca tccactgcgc gccgcccggc ccgagcaggc cggcgttgcc ggcggtatcg
      901 tggtggcgca tgcggccggc gagcatgtag gtgacggcct cgaagccgcg gtgcgggtga
      961 tcgggaaagc cgccgatgta gtcctccggg ttctcgttgc ggaaggcgtc gagcatcagg
     1021 aaggggtcga gccggcgttg caggttctgc gtcagcacgc gggtcaggcg caccccggcg
     1081 ccgtccgagg tcggcttgcc ctggaccagg cgctcgactc ggcggctttg caggacggtg
     1141 gatagcgttg cggtcatggc atcagtctcc attggggttg gggcgattgg cggcgtggtc
     1201 agcaatcaga ccacgacttc ctcgatccgt gccgcggcct cggcaaaggc cttgctggcc
     1261 gagtcggcac ccatcgccag accttcggcg tagatgaact cgacgtcggt catgccgagg
     1321 aaggcaagca cgctcttcag gtaaggcacc tgcgaatcgg ccggggtatc gcggtagagg
     1381 ccgccgcggg ccagcgcgac atagaccttc ttgcccttga gcagcccttc cggcccgttg
     1441 gcggtgtagc ggaaggtgac gccggcgcgg gcgatggcgt cgatccaggt cttcagttgc
     1501 acgggcacgc cgaagttgta catcggcacg ccgagcacga tcacatcgac cgcctgcacc
     1561 tgggcgatca gcgcgtcgtc caacgcgacg cgggc
//
LOCUS       DAOSDX010000757         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25814_length_1594_cov_3.143600, whole genome shotgun sequence.
ACCESSION   DAOSDX010000757 DAOSDX010000000
VERSION     DAOSDX010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25814_length_1594_cov_3.143600"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1277)
                     /locus_tag="PLN02_13990"
     CDS             complement(21..1277)
                     /locus_tag="PLN02_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOI53487.1"
                     /translation="MSPELAAPLIAIETHPLQTAIVGVFAVVYLGMFLGGLPKLKLDR
                     SGVALLGGIAVVGIGAMTTEEAARAVHLPTVLLLFAFMVVSAQMRLGGFYTEVTRRVA
                     ALPLAEPWLLGALIFVAGALSAVFSNDIVCLAMTPVVARLCIERRLDPVPFLVGLACA
                     ANIGSAATLIGNPQNMLIGSVLQLHFADYLWRALPPVVLGLFALWAWLVFAPRRATLP
                     PAAAPATEALPEFDRLQTAKGLAVAAVLLVVFLGTDWPREVAALVGAGVLLLSRRFHS
                     SHVMGFVDWELLVLFVGLFVVNQAFDSTGLAAIAVAWLAGHGIHLGDAGPLFAVGVGL
                     SNLVSNVPAVMLLLPHLKGPDAGLTLALVSTLAGNLLLVGSIANLIVVDLARKQGIAI
                     DWRQHLRIGLPVTALTLAIAWIWLNL"
     gene            complement(1274..>1594)
                     /locus_tag="PLN02_13995"
     CDS             complement(1274..>1594)
                     /locus_tag="PLN02_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOI53488.1"
                     /translation="KFQGQLCQALQIHSEGPYYDHAAFTPWRLQALAFKAIRRLYPDY
                     PIWRDFGYEYEFDRLAIDLINGSPLLRQWVDDPAAAPADLERAVLADESLWVARREAF
                     LLYR"
BASE COUNT          299 a          512 c          582 g          201 t
ORIGIN      
        1 ccattcggtt cccaagtttt ttacagattc agccagatcc aggcgatcgc cagcgtcagc
       61 gccgtcaccg gcaggccgat gcgcaggtgc tggcgccagt cgatggcgat cccctgcttg
      121 cgggcgaggt cgacgacgat caggttggcg atcgagccga ccagcagcag gttgccggcc
      181 agcgtgctga ccagcgccag ggtgaggccg gcgtccgggc ctttcaggtg tggcagcagc
      241 agcatcaccg ccggcacgtt cgacaccagg ttggagaggc cgacgccgac cgcgaacagc
      301 gggccggcat cgcccaggtg gatgccgtgg ccggccagcc aggcaacggc gatggcggcc
      361 aggccggtgc tgtcgaaagc ctgattgacg acgaacaggc cgacgaagag caccagcagt
      421 tcccaatcga cgaagcccat cacgtgcgat gagtgaaaac gccgcgacag cagcagcacg
      481 ccggcgccga ccagggcggc gacttcgcgc ggccagtcgg tgcccaggaa gacgaccagc
      541 agcacggcgg cgacggcaag acccttggcg gtctgcaagc ggtcgaactc gggcagcgcc
      601 tcggtggccg gggcggcggc cggcggcagg gtggcgcggc gcggcgcgaa gaccagccag
      661 gcccatagtg cgaacaggcc gaggacgacc ggcggcagtg cccgccacag gtagtcggcg
      721 aaatgcagtt gcagcaccga gccgatcagc atgttctgcg ggttgccgat caaggtcgcc
      781 gccgagccga tgttggcggc gcaggcgagg ccgaccagga agggcaccgg gtcgagccgg
      841 cgctcgatgc acaggcgggc gaccaccggc gtcatcgcca ggcagacgat gtcgttggag
      901 aacaccgccg acagcgcgcc ggcaacgaag atcagggcgc cgagcagcca cggttcggcg
      961 agcggcagcg cggcgacgcg gcgggtgact tcggtgtaga agccgcccag gcgcatctgc
     1021 gccgaaacca ccatgaaggc aaacaacagc aggaccgtcg gcagatgcac ggcgcgcgcc
     1081 gcttcttcgg tcgtcatcgc gccgatgccg acgacggcga tgccgccgag cagcgcgaca
     1141 ccgctgcggt cgagcttcag cttcggcagg ccgccgagga acatgccaag gtagacgacg
     1201 gcgaagacgc cgacgatggc cgtctgcagc gggtgcgtct cgatggcgat gagcggtgca
     1261 gcgagttccg ggctcatcga tagagcagga atgcctcgcg tctggcaacc cacaggcttt
     1321 cgtcggcgag cacggcgcgc tccaggtcgg ccggggcggc ggccgggtcg tccacccatt
     1381 ggcgcagcag cggcgaaccg ttgatcaggt cgatcgccag gcgatcgaat tcgtactcgt
     1441 agccgaaatc gcgccagatc gggtaatccg ggtaaaggcg gcggatcgcc ttgaaggcca
     1501 gcgcctgcag gcgccagggc gtgaacgcgg catgatcgta gtaggggcct tcgctgtgta
     1561 tctgcagcgc ctggcagagc tgcccctgga actt
//
LOCUS       DAOSDX010000758         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25905_length_1590_cov_3.008469, whole genome shotgun sequence.
ACCESSION   DAOSDX010000758 DAOSDX010000000
VERSION     DAOSDX010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25905_length_1590_cov_3.008469"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLN02_14000"
     CDS             <1..448
                     /locus_tag="PLN02_14000"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HOI53489.1"
                     /translation="VGLMMWTPLLAVLFNRFFFHPMVPAKLLPTLFILIAPPAVGCIA
                     WLKLHHGVLDDVARILYYFGLFITLLLFFQWRHFARLSFALPWWAYSFPLAAMTIASS
                     IMLEKVGGTFFAILTPVLLGLLSILIVVLLFNTSRAMLRGEICVPE"
     gene            551..>1590
                     /locus_tag="PLN02_14005"
     CDS             551..>1590
                     /locus_tag="PLN02_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOI53490.1"
                     /translation="MDVLFLLALATVIVVLLFDYTNGFHDAANIVATVIASRAMTPVQ
                     AVIVVGFFEFLGPLLGGTAVANTIGKFVSLDGVVPLLSLSILLCGLIGAIVWNLATWY
                     FGIPSSSSHALVGGLIGAIVVSVGSDHVVWGFRELADGHLTGIIKVLAALVLSPLIGF
                     WAGFLIHRLMNALLRSASTAANTRLRYAQFFTAAGLAFSHGANDAQKSMGILTLVLLL
                     GGFIPSFEVPFWVMLACASAITLGIISGGWRIVRTLGFAIYRVRPIHALDSQLTSALV
                     IMGASLAGAPVSTTHVVATSIMGIGASERPRAVRWAKAKDIATTWVITIPGAALVAIQ
                     AYALLHLFIGVPS"
BASE COUNT          222 a          568 c          449 g          351 t
ORIGIN      
        1 cgtcggcctg atgatgtgga cgccgctgct ggcggtgctg ttcaaccgct tcttcttcca
       61 tccgatggtc ccggccaagc tgctgccgac actgttcatc ctgatcgccc cgccggcagt
      121 cggctgcatc gcctggctca agctgcacca cggcgtgctc gatgacgtgg cgcgcatcct
      181 ctactacttc ggtcttttca tcaccctgct gctgttcttc cagtggcgcc actttgcccg
      241 cctctcgttc gcccttccct ggtgggccta ttccttcccg ctggcggcga tgaccatcgc
      301 ctccagcatc atgctcgaaa aggtcggcgg cacctttttt gccattctga ccccggtgct
      361 gctgggctta ctgagcatcc tgatcgtcgt gctgctgttc aatacgagcc gggccatgct
      421 gcgcggcgag atctgcgttc cggaataagg cagcccctcg tttcccgccc ctccggcaag
      481 aagagcgggc tatactggcg ggaaacgaga cataacgaat ccggaggagg cccgcccgcg
      541 ggcaacgtgc atggatgttc tattcctgct cgcactggca accgtcatcg tcgtcctgct
      601 cttcgactac accaacggtt tccacgatgc cgccaacatc gttgcaacag tcatcgcctc
      661 ccgggcgatg actccggtgc aggcggtgat cgttgtcggt ttctttgaat tcctcggccc
      721 gctgctcggc ggcaccgcag tcgccaacac gatcggcaag ttcgtcagcc tcgacggcgt
      781 cgtaccgctg ctctcgctgt cgatcctgct ttgcggactg atcggtgcca tcgtctggaa
      841 cctggcgacc tggtatttcg gcattccctc gtcgtcttcg cacgcgctgg tcggcggact
      901 gatcggcgcc atcgtcgtca gcgtcggcag cgatcatgtc gtctggggct tccgcgagct
      961 tgccgacggc cacctgaccg gcatcatcaa ggttctggcc gccctcgtcc tgtccccgct
     1021 gatcggcttc tgggccggct tcctgatcca tcgcctgatg aacgccctgc tacgcagcgc
     1081 cagtaccgcc gccaacaccc gactgcgtta tgcccagttc ttcactgccg ccggcttggc
     1141 gttttcgcac ggtgccaacg acgcccagaa gagcatgggc atcctgaccc tggtcctgct
     1201 gctgggcggc ttcatcccca gcttcgaagt cccgttctgg gtcatgctcg cctgtgcctc
     1261 ggcgatcacc ctcggcatca tttccggcgg ctggcgcatc gtccgtacgc tcggcttcgc
     1321 catctaccgg gtgcggccga tccacgcgct ggattcgcaa ctgacctcag cgctagtgat
     1381 catgggcgca tcgctggccg gcgccccggt gtcgacaacg catgtcgtcg ccacctcgat
     1441 catgggcatt ggcgcctcgg aacggccgcg tgcggtccgc tgggccaagg ccaaggacat
     1501 cgcgaccacc tgggtgatca ccatccctgg cgccgccctg gtcgccatcc aggcctacgc
     1561 cctgctccac ctgttcatcg gagtcccatc
//
LOCUS       DAOSDX010000759         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25924_length_1589_cov_3.571708, whole genome shotgun sequence.
ACCESSION   DAOSDX010000759 DAOSDX010000000
VERSION     DAOSDX010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25924_length_1589_cov_3.571708"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..807
                     /locus_tag="PLN02_14010"
     CDS             199..807
                     /locus_tag="PLN02_14010"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HOI53491.1"
                     /translation="MKLIGSHTSPYTRKVRVVLAEKKKEYEFIVDHPRSPDSSVPDLN
                     PLGRIPTLLLDDDTALFDSRVIVEHIDNITPNNKLLPAPNRERTEVKRWEALADGLCD
                     AAVSAVMEGLRPANEQSPDWIARQRDAIARTLRFMETHLGEKSYCMGTHFSLADIAVG
                     VALGYLCLRFPDLDWVTDHPNLARLYEKLMQRQSFIDTVPVV"
     gene            822..1169
                     /locus_tag="PLN02_14015"
     CDS             822..1169
                     /locus_tag="PLN02_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53492.1"
                     /translation="MLRKRHLTLLLSFALGATRPAIAGMQVTCPVINHEEVYLVSIYD
                     GKPSEEVSLASDDDERTPDLYTLGHIYDAGRSVTIRCTYKSGAVHDIELKQKVQACRT
                     SRGKTGKLRFNCQ"
     gene            1344..>1589
                     /locus_tag="PLN02_14020"
     CDS             1344..>1589
                     /locus_tag="PLN02_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOI53493.1"
                     /translation="MNRKLNSLCIALAERDPETRSHSDRVADLALELGRHIALRDDEL
                     DLLALGACLHDIGKIGIPDRVLLKPGRFEPAERETMRQ"
BASE COUNT          336 a          546 c          452 g          255 t
ORIGIN      
        1 gcccggatca ggccgaattc ggagaagtgt tcgcgcagcg catcgacctt gccggcgtag
       61 cggccatcca ggggggaaag cgcggtcagg gggttgaatt ccatggaagc atccttatcg
      121 ttaagggcag gattttaccc gcccgaccgc accctgcgcc atacgctata attgcgcacc
      181 ccaatcccag agcctgtaat gaagctgatc ggttcgcaca ccagccccta tacccgcaaa
      241 gtacgggtcg tactggcgga aaagaagaag gaatacgagt tcatcgtcga ccacccgcgg
      301 agtccggaca gttcggtacc cgacctcaat ccgctgggac gcatcccgac cctgctgctc
      361 gacgacgaca cggcactgtt cgattcccgc gtgatcgtcg agcacatcga caacatcacg
      421 ccgaacaaca agctgttgcc ggcgcccaac cgcgaacgca ccgaggtcaa gcgctgggag
      481 gcgctggccg acggactctg cgacgccgcc gtcagcgccg tgatggaagg cctgcgcccg
      541 gccaacgaac agagcccgga ctggatcgcc cgccagcgcg acgcgatcgc ccgcaccctg
      601 cgcttcatgg aaacccacct cggcgagaaa agctactgca tgggcaccca cttctcgctc
      661 gccgacatcg ccgtcggcgt cgccctgggc tacctctgcc tgcgcttccc cgacctcgac
      721 tgggtgaccg accacccgaa cctggcccgc ctgtacgaga aactgatgca gcgccagtcg
      781 ttcatcgaca ccgttcctgt cgtctgaagc tcgtcgccac catgttgcgg aaaagacatc
      841 tgacactgtt actctcgttc gccctgggcg cgacccgccc tgccattgcc ggaatgcagg
      901 tcacgtgccc ggtaataaac cacgaagagg tttatctggt ttccatctac gacggcaaac
      961 ctagcgaaga agtcagtctg gcttccgacg acgacgaacg cacaccggat ctctatacac
     1021 tcggtcacat ctacgacgcc ggcagaagcg tgacgattcg ttgcacctac aaaagcggcg
     1081 cggtgcacga tatcgaactg aagcaaaagg tgcaggcgtg ccggacttcc cgaggcaaaa
     1141 caggaaaact ccgcttcaat tgccaatagc agctggcata cctgcgccag caacaagccg
     1201 ctagcggcgc ccgccctgca gcagcagcgt tcctgccata cgatccgcgg cgcatggcac
     1261 gacgcagcgc cggctaccgc agtttttttc gaactcgcgc cccgcggtga ggtcaacctg
     1321 gcattccgga gacaggcagg cccatgaacc gcaaactgaa ttccctatgc atcgccctcg
     1381 ccgagcgcga cccggagacg cgcagccact ccgaccgggt ggccgacctg gcgcttgaac
     1441 tgggtcgcca tatcgcgctg cgcgacgacg agctcgacct gctggcgctc ggcgcctgcc
     1501 tgcacgacat cggcaagatc ggcattcccg accgcgttct gctcaagccg ggacgcttcg
     1561 agccggcgga aagggagacg atgcgccag
//
LOCUS       DAOSDX010000760         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25963_length_1588_cov_2.037834, whole genome shotgun sequence.
ACCESSION   DAOSDX010000760 DAOSDX010000000
VERSION     DAOSDX010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25963_length_1588_cov_2.037834"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PLN02_14025"
     CDS             <1..1332
                     /locus_tag="PLN02_14025"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53494.1"
                     /translation="VACRVYGHIKARHLWDMIMVSTYDYAEPGFILIDRVNEMNNNWF
                     CENIRATNPCGEQPLPPYGACLLGSVNLTKFVREPFTDKAWFDWDEYREVVATFTRML
                     DNVVEVNGLPLQQQRDEIMRKRRHGMGYLGLGSTLTMLKMKYGEAESLKFTEEVTKVM
                     AEVGWQVGIDLAKEKGPAPIMEEEFVVTADMLAKRPEMKADGIKLGAKVKGKVLMGLY
                     SKYMQQFPENLRKEIAKNGVRFTHHSSIAPTGTISLSLANNASNGIEPSFAHHYARNV
                     IREGKKSKEKVDVFSYELLAYRELVNPHAMPFSDKPEEQLPDYFLDSSTIQAKAHVDI
                     QAAAQKWIDSSISKTINVPTDYDFEDFKNIYLYAYDKGLKGCTTFRFNPEAFQGVLVT
                     EKDLENTTYKFTLEDGTVIEAKGNEEIEYDGEIHSAANLYDALKEGYYGKF"
BASE COUNT          411 a          498 c          434 g          245 t
ORIGIN      
        1 gtggcctgcc gggtgtacgg ccacatcaag gcgcggcacc tgtgggacat gatcatggta
       61 tccacctacg actacgccga gccgggcttc atcctgatcg accgcgtcaa cgagatgaac
      121 aacaactggt tctgcgagaa catccgcgcc accaacccct gcggcgaaca accgctgccg
      181 ccttacggtg cctgtctgct gggctcggtc aacctgacca agttcgtgcg cgagccgttc
      241 accgacaaag cctggttcga ctgggacgaa taccgtgaag tggtcgccac cttcacccgc
      301 atgctggaca acgtggtcga ggtcaacggt ctgccgctgc agcaacagcg cgacgagatc
      361 atgcgcaagc gccgccacgg catgggctat ctgggcctcg gctccacgct gaccatgctc
      421 aagatgaaat acggcgaagc cgaatcgctc aagttcaccg aggaagtgac caaggtcatg
      481 gccgaagtgg gctggcaagt cggtatcgat ctggccaagg aaaaaggccc ggcaccgatc
      541 atggaagaag agttcgtcgt caccgccgac atgctggcca agcgtccgga aatgaaggcc
      601 gacggcatca agctcggcgc caaggtcaag ggcaaggtgc tgatgggcct ctatagcaaa
      661 tacatgcagc agttcccgga aaacctgcgc aaggagatcg ccaagaacgg cgtgcgcttc
      721 acgcaccaca gctccatcgc gccgaccggc accatctcgc tgtcgctcgc caacaacgcc
      781 agcaacggca tcgaaccttc gttcgcccac cactatgcgc gcaacgtcat ccgtgaaggc
      841 aagaagtcca aggaaaaggt cgacgtcttc tcctacgaac tgctggcgta ccgcgagctg
      901 gtcaacccgc acgccatgcc gttctccgac aagccggaag aacagttgcc ggactacttc
      961 ctcgattcct cgacgatcca ggccaaggcc cacgtcgaca ttcaggccgc cgcgcagaaa
     1021 tggatcgaca gctcgatttc caagaccatc aacgtgccga ccgactatga tttcgaggac
     1081 ttcaagaaca tctacctcta cgcctacgat aagggcctga agggctgcac caccttccgc
     1141 ttcaacccgg aagccttcca gggcgtgctg gtgaccgaga aggatctgga aaacaccacc
     1201 tacaagttca cgctggaaga cggcaccgtc atcgaggcca agggcaacga agagatcgaa
     1261 tacgacggcg agatccacag cgccgccaac ctgtacgacg cgctgaagga aggttactac
     1321 ggcaagttct gatcaagtaa agggagaagg gtgaagcgta aagggtaagc cccttacccc
     1381 cttcgtcatc ccaaccaagg agaataaaca atggcaatca aaatcgaaaa gaaaatcacc
     1441 ggctacaacg tcgtcaccga agaagacaag gccaaggcgg ccgaagccga tctgctggca
     1501 tcgcagaaaa ccgccgagat cctgcaactg ggcgaaccgc tgagccgccc ggacaaactg
     1561 gtcggcaaca cctacaagat caagaccc
//
LOCUS       DAOSDX010000761         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_25968_length_1588_cov_1.807567, whole genome shotgun sequence.
ACCESSION   DAOSDX010000761 DAOSDX010000000
VERSION     DAOSDX010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25968_length_1588_cov_1.807567"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1588)
                     /locus_tag="PLN02_14030"
     CDS             complement(<1..>1588)
                     /locus_tag="PLN02_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI53495.1"
                     /translation="PEAESCPKCKTPLPPGAEECPICNREATVAPSTWTLFRLWRFAR
                     PYRWQLFLGFVLTLASTAAQLVPPYLTMPLMDNVLIPFQNGQPIDWNLVGMYLGGLLG
                     AAIVAWGLGWIRTYILSLVSERMGRDLRTQTYDHLLGLSLEYFGGKRTGDLMARIGNE
                     TDRINIFLSLDLLNFATDVLMITMTAVILFSINPWLALVTLLPLPIIGWLIHFVREKL
                     RTGFEKIDRVRAEVTNVLADTIPGIRVVKAFAQEKREGERFRAANEHNLQMNDKLNRT
                     WSLFTPTVTLLTEVGLLVVWVFGIWQIAHGDSTVGTLTAFLAYIGRFYTRLDSMSRIV
                     SATQRAASSTKRIFDILDHVSSVPEPTNPVHLQNVTGRIELKQACFRYGTRAVTKDVD
                     LVIEPGEMIGLVGHSGSGKSTLVNLICRFYDVTEGQMLIDGVDVRSVPVAEFRSHIGL
                     VLQEPFLFFGTIADNIAYGKPDATRAEIVAAARAAHAHEFILRLPHGYDSLVGERGQG
                     LSGGERQRISIARALLIDPK"
BASE COUNT          270 a          459 c          593 g          266 t
ORIGIN      
        1 atcttcgggt cgatgagcag cgcgcgggcg atcgagatgc gctggcgctc gccgccggac
       61 agcccctggc cgcgttcgcc gaccagcgag tcgtagccgt gcggcaggcg caggatgaat
      121 tcgtgggcgt gggcggcgcg ggcggcggcg acgatttcgg cgcgcgtcgc gtcgggtttg
      181 ccgtaggcga tgttgtcggc gatggtgccg aagaacagga agggttcctg cagcaccagg
      241 ccgatgtggc tgcggaattc ggcgaccggc accgagcgca catcgacgcc gtcgatcagc
      301 atctggccct cggtgacgtc gtagaagcgg cagatcaggt tgaccagcgt cgacttgccg
      361 gagccggaat gaccgaccag tccgatcatc tcgcccggtt cgatgaccag gtcgacatcc
      421 ttggtgacgg cgcgggtgcc gtagcggaag caggcctgct tgagttcgat gcggccggtg
      481 acgttttgca gatgtaccgg gttggtcggt tccggcacgc tcgagacgtg gtcgaggatg
      541 tcgaagatgc gcttggttga cgacgcggcg cgctgcgttg ccgagacgat gcggctcatc
      601 gagtcgaggc gggtgtagaa gcggccgatg taggcgagga aggcggtcag cgtaccgacc
      661 gtcgaatcgc catgggcgat ctgccagatg ccgaagaccc agacgacgag caggccgacc
      721 tcggtgagca gcgtcaccgt cggggtgaac agcgaccagg ttcggttgag cttgtcgttc
      781 atctgcaggt tgtgctcgtt ggcggcacgg aagcgctcgc cctcgcgctt ttcctgggca
      841 aaggccttga ccacgcggat gccggggatg gtgtcggcca gcacgttggt cacttcggcc
      901 cggacgcggt cgatcttctc gaaaccggtg cgcagcttct cgcgcacgaa gtggatcagc
      961 cagccgatga tcggcaaggg gagcagggtg accagcgcca gccaggggtt gatgctgaac
     1021 aggatgaccg cggtcatcgt gatcatcagc acgtcggtgg cgaagttgag caggtcgagc
     1081 gagaggaaga tgttgatgcg gtcggtttcg ttgccgatgc gcgccatcag gtcgccggtg
     1141 cgcttgccgc cgaaatattc gagcgacagg ccgagcaggt ggtcgtaggt ctgggtgcgc
     1201 aggtcgcggc ccatgcgctc ggagaccagc gacaggatgt aggtgcggat ccagcccagt
     1261 ccccaggcga cgatggccgc gccgagcagg ccgccgaggt acatgccgac caggttccag
     1321 tcgatcggct ggccgttctg gaacgggatc agcacgttgt ccatcagcgg catcgtcaga
     1381 tagggcggca ccagctgtgc cgcggtcgag gccagggtga gcacgaaacc gaggaagagc
     1441 tgccagcggt agggccgggc gaaacgccag agacggaaca gggtccaggt cgacggggcg
     1501 accgtcgcct cgcggttgca gatcgggcat tcctcggcgc ccggcggcag cggcgtcttg
     1561 cacttcgggc agctttccgc ctccggcc
//
LOCUS       DAOSDX010000762         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26005_length_1586_cov_2.969301, whole genome shotgun sequence.
ACCESSION   DAOSDX010000762 DAOSDX010000000
VERSION     DAOSDX010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26005_length_1586_cov_2.969301"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..289
                     /gene="rpsT"
                     /locus_tag="PLN02_14035"
     CDS             14..289
                     /gene="rpsT"
                     /locus_tag="PLN02_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOI53496.1"
                     /translation="MANSAQARKRARQADKQRAHNASLRSTLRTAIKSVRKAIVAGDK
                     AAAQSIFQQSVSVIDRIADKQIVHKNMVSRTKSRLSAQIKAMGAAAA"
     gene            complement(357..656)
                     /locus_tag="PLN02_14040"
     CDS             complement(357..656)
                     /locus_tag="PLN02_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HOI53497.1"
                     /translation="MTTQESTTFVIVGVTKDGRKFRPSDWAERLCGVMSAFGTERKMR
                     YSPYVGPGDYNGDKAVFVDGRLGEIEPMAYRFMLNFAQDNDLQIIDGVCPLDGKK"
     gene            complement(861..>1586)
                     /locus_tag="PLN02_14045"
     CDS             complement(861..>1586)
                     /locus_tag="PLN02_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HOI53498.1"
                     /translation="AKTPGDAFEMHMPPHGEKKRMPSVVSAMAQVIHWRVNQLGALGH
                     DGPTPVKDALFSTKEPKTGTDGTLSWTVDVNNPSTGEDFVLGLKEITLPDGVTRPYSM
                     WLSGNYPRALDGLCKLLSLDMRVIDPAWIGMKLRKLLDYSEPLGDFMAFVPGSRKQQT
                     WPSTVAYIAQLIIHRYAMLGVLDEEGFPLQQMGILEAPEDTTSNKVLPTQGKLCGECG
                     NHTMIRKDGCDFCTACGAVGTCG"
BASE COUNT          287 a          463 c          517 g          319 t
ORIGIN      
        1 ctggatttaa ttcatggcca atagcgcaca agcccgcaag cgcgcccgtc aagctgacaa
       61 gcagcgcgcc cacaatgcaa gtctgcgttc gaccctgcgt accgcgatca agagcgtgcg
      121 caaggcgatc gttgccggcg acaaagccgc cgcacaaagc attttccaac aatccgtttc
      181 ggtcatcgat cgtatcgctg acaagcagat tgtgcacaag aatatggtgt ctcgcaccaa
      241 gagccgtctg tctgcacaga tcaaggcgat gggcgctgcc gctgcctgat cgaggcgact
      301 cccggaatca taaaaatggc gcctgcgggc gcctttttta ttgcttctag attgctctat
      361 tttttgccgt ccagcgggca gacgccgtcg atgatctgca gatcgttgtc ctgggcgaag
      421 ttgagcatga agcggtaagc catcggctcg atttcgccga ggcggccatc gacgaaaacg
      481 gccttgtccc cgttgtaatc gccggggccg acgtagggcg agtagcgcat cttgcgttcg
      541 gtgccgaacg ccgacatgac gccgcacagg cgttcggccc agtcgctggg ccggaatttc
      601 ctgccatcct tggtcacgcc gacgatgacg aaggtggtcg attcttgagt tgtcattgtg
      661 tactctgctt ataggggatg cgaccttgcg tatccgcgca ttctagcaga agcgagtggt
      721 cttaccataa ctgcaagccc ttgccggcag gctgcaaagg cgccttgggc ggccgttcgc
      781 gcgggcattc cagatgcaag aaggcccatc gagcttatgg tcgatgggcc ttcgggggat
      841 tacccggtgc gaatctcagg tcagccgcag gtgccgaccg caccgcaggc ggtacagaag
      901 tcgcagccgt ccttgcggat catcgtgtgg ttgccgcatt cgccgcacag cttgccctgg
      961 gtcggcagca ccttgttgct ggtggtgtct tccggcgcct cgaggatgcc catctgctgc
     1021 agcgggaagc cttcctcgtc gagcacgccg agcatggcgt agcggtggat gatcagctgg
     1081 gcgatgtagg cgacggtgga gggccaggtc tgctgcttgc gcgaaccggg cacgaaggcc
     1141 atgaaatcgc cgagcggttc ggagtagtcg agcagcttcc tgagcttcat gccgatccag
     1201 gccggatcga tgacgcgcat gtcgagcgag aggagcttgc acaggccgtc gagggcgcgc
     1261 gggtagttgc ccgaaagcca catcgaatag gggcgggtga cgccgtcggg cagggtgatt
     1321 tccttcaggc cgaggacgaa atcctcgccg gtcgacgggt tgttgacgtc aaccgtccag
     1381 gacagcgtgc cgtcggtacc ggtctttggt tccttggtcg agaacagggc atccttgacc
     1441 ggcgtcgggc cgtcgtggcc gagggcgccg agctggttga cgcgccagtg aatgacctgc
     1501 gccatcgccg agacgaccga cggcatgcgc ttcttctcgc cgtgcggcgg catgtgcatt
     1561 tcgaaggcgt cgcccggcgt cttggc
//
LOCUS       DAOSDX010000763         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26044_length_1584_cov_3.270765, whole genome shotgun sequence.
ACCESSION   DAOSDX010000763 DAOSDX010000000
VERSION     DAOSDX010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26044_length_1584_cov_3.270765"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1584)
                     /locus_tag="PLN02_14050"
     CDS             complement(<1..>1584)
                     /locus_tag="PLN02_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOI53499.1"
                     /translation="RPRVGLVLGGGGARGAAHLGILEALEEQRIAVDCVAGTSMGALF
                     AGAWAAGVTPAAMRRELERVDWRDIFIDNPEYGEMGYRNRLIARTYPPGSEGGVGDKG
                     VRYQGGVVAGQKIKLFLNRLVGTVRQIESLPLPLSIVATDIGNGQRVVFRDGTLSTAM
                     RASMSVPGLLAPVETGGRKLVDGGLVDNLPVAEVRERCQADVVIAVDVGTPLLKSEEI
                     GSLLTVSAQMIGILTQQNVERSLALLGPRDILIRPDLGDITAVDFPRHEEAAERGRRA
                     AQALAERLAGLRLSPADYAAWQQAMTVEPQPQLRVDAVEVAGLHRVNPQAVERLLDIA
                     PGEEVDALRIDNNLLRIYGDGHYESVDYALSNEAGRNLLRVMPVEKSWGPDYLRFGLT
                     LQADDGDGSSFGLRGAYHQTWLNALGGELLYHAELGSRDRLGVNYYQPLDPGQRVFFE
                     ALAGVEQEDINIYRDDRRLAQYRARQGLLGVWLGHNLGVYGSVRLGWLHRDRRYRLDT
                     GQPVIPSANTEFGGWQAGI"
BASE COUNT          234 a          563 c          544 g          243 t
ORIGIN      
        1 tcgatcccgg cctgccagcc gccgaattcg gtattggcgc tgggtatcac cggttgaccg
       61 gtatccaggc ggtaacggcg gtcgcgatgc aaccagccga gacggacgct gccatagacc
      121 ccgaggttgt gtcccagcca gaccccgagc agcccctgcc tggcccggta ctgggcgagg
      181 cgccgatcgt cccggtatat gttgatgtcc tcctgttcga cgccggccag cgcctcgaag
      241 aagacgcgct gcccgggatc gagcggctgg tagtaattga cgccgaggcg gtcgcgcgag
      301 cccagttcgg cgtgatagag cagttcgccg cccaaggcgt tgagccaggt ctggtgatag
      361 gcgccgcgca ggccgaagga ggagccgtcg ccgtcgtccg cttgcaaggt caggccgaag
      421 cgcaggtagt ccggccccca ggacttctcg accggcatca cgcgcagcag gttgcgcccg
      481 gcttcgttgc tcagcgcgta atcgacgctc tcgtagtggc cgtcgccata gatgcgcagc
      541 aggttgttgt cgatacgcag cgcgtcgact tcctcgcccg gcgcgatgtc gagcaggcgc
      601 tcgaccgcct gcgggttgac ccggtgcaga ccggccactt cgaccgcatc aacgcgcagt
      661 tgcggctgcg gctcgaccgt catcgcctgc tgccacgcgg catagtccgc cgggctcagg
      721 cgcagccccg ccaggcgttc ggccagcgcc tgcgcggcgc gccgtccgcg ttcggcggcc
      781 tcctcgtgac gcgggaaatc gaccgccgtg atgtcgccga ggtcggggcg gatcaggatg
      841 tcgcgcggcc cgagcagggc cagtgagcgc tcgacgttct gctgggtgag gatgccgatc
      901 atctgcgccg agacggtcag cagcgaaccg atctcctcgc ttttcagcag gggcgtcccg
      961 acatcgacgg cgatcacgac gtcggcctgg cagcgctcgc gcacctcggc gaccggcaga
     1021 ttgtcgacca ggccgccatc gaccagcttg cgcccgcccg tctcgaccgg cgccagcagg
     1081 ccgggcaccg acatgctggc gcgcatcgcc gtgctcagcg tgccgtcgcg gaagaccacc
     1141 cgctggccgt tgccgatatc ggtggcgacg atcgacagcg gcagcggcag gctttcaatc
     1201 tggcgcacgg tgccgaccag gcggttgagg aagagcttga tcttctggcc ggcgacgacg
     1261 ccgccctggt aacgcacccc cttgtcgccg acgccgccct cggagcccgg cgggtaggtg
     1321 cgggcgatca gccggttgcg gtaacccatc tcgccgtatt ccgggttgtc gatgaagatg
     1381 tcgcgccaat cgacccgctc cagttcgcgg cgcatcgccg ccggcgtcac gccggccgcc
     1441 caagcaccgg cgaagagggc acccatgctg gtgccggcga cgcaatcgac cgcgatgcgc
     1501 tgttcttcga gcgcctccag gatgcccagg tgggcggcgc cgcgcgcccc gccgccgccg
     1561 agcaccagcc cgacccgcgg ccgt
//
LOCUS       DAOSDX010000764         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26045_length_1584_cov_3.204055, whole genome shotgun sequence.
ACCESSION   DAOSDX010000764 DAOSDX010000000
VERSION     DAOSDX010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26045_length_1584_cov_3.204055"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1092)
                     /locus_tag="PLN02_14055"
     CDS             complement(82..1092)
                     /locus_tag="PLN02_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HOI53500.1"
                     /translation="MRYQVTVQPSGRQFEAEADESLLDAALRQGLTMPYGCKDGACGA
                     CKGKVLDGTVEHRHAAALSEDEKAAGLALYCCAQASSDLVIECKQLRSAHDIPVKTLP
                     ARIEKLEKLAPDVIEMHLRLPAAEHLQFLAGQYIDILLKDGRKRSFSLANAPYEDNLL
                     QLHIRHVPGGFFTEQVFTTMKVRDILRFDGPHGSFSLHNDAGKPVILLAGGTGFAPIK
                     SIVEQAIADRYPHPLHIYWGAKARVDLYQNALPEMWAASQANIHYVPVLSEPAADDAW
                     TGRTGFVHHAVLADFPDLSGHQVYACGSPVMIDIARKDFISQCGLPEEEFFSDAFTFS
                     AS"
     gene            1126..>1584
                     /locus_tag="PLN02_14060"
     CDS             1126..>1584
                     /locus_tag="PLN02_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOI53501.1"
                     /translation="MQKILIVGSGDVARRILTQLAPHYRVYALLRDPARHADWRRAGA
                     LPVAGDLDERRSLGRLGGLADIVLHLAPPPASGHLDSRTQHLLAALESAKSLPRRLIY
                     VSTTGVYGDCAGAWIDETRACSPGNARAQRRVDAERQLRAWGARRGVAVSI"
BASE COUNT          247 a          497 c          568 g          272 t
ORIGIN      
        1 ccaggttgcc cggtcccggg atcgccagga agatcagcac agcggcgacg aaagcgccgt
       61 agtcggcgac gccgaacatg ctcagcttgc cgagaaggtg aaggcgtcgg agaagaactc
      121 ttcttccggc aggccgcact ggctgatgaa gtccttgcgc gcgatgtcga tcatcaccgg
      181 cgagccgcag gcatagacct ggtggccgga gaggtcgggg aaatcggcca gcacggcgtg
      241 atggacgaaa ccggtacggc cggtccaggc gtcgtcggcg gccggctcgg aaaggaccgg
      301 cacgtagtgg atgttggctt ggctggcggc ccacatttcc ggcagcgcgt tctgatagag
      361 gtcgacgcgc gccttggcgc cccagtagat gtgcagcggg tgcgggtagc ggtcggcgat
      421 cgcctgttcg acgatggact tgatcggcgc gaaaccggtg ccgccggcga gcaggatgac
      481 cggcttgccg gcgtcgttgt gcaggctgaa gctgccgtgc gggccgtcga agcgcaggat
      541 gtcgcgcacc ttcatcgtgg tgaatacctg ttcggtgaag aaaccgccgg gtacgtgccg
      601 gatgtgcagt tgcagcagat tgtcctcgta gggcgcgttg gccagcgaga agctgcgctt
      661 gcggccatcc ttgagcagga tgtcgatgta ctggccggcg aggaactgca ggtgttcggc
      721 cgccggcaga cgcaggtgca tctcgatgac gtcgggcgcc agcttttcca gcttctcgat
      781 gcgggcgggc agggtcttga ccgggatgtc gtgcgccgag cgcagttgct tgcactcgat
      841 gaccaggtcg gaactggctt gggcgcagca gtagagcgcc agaccggcgg ccttttcgtc
      901 ttcgctcaag gccgcggcgt gacggtgttc gaccgtgccg tcgagcacct tgcccttgca
      961 ggcaccgcag gcgccgtcct tgcagccgta gggcatggtc agcccctggc gcagcgcggc
     1021 atcgagcagc gattcatcgg cttcggcctc gaactggcgg ccgctcggct ggactgtcac
     1081 ttggtagcgc atcggttccc cttggtcgtc ggctacaatg ccgacgtgca aaaaatcctg
     1141 attgtcggca gcggggacgt cgcccgccgc atccttaccc agcttgcgcc gcattaccgc
     1201 gtctatgccc tgctgcgcga cccggcacgc catgcggact ggcgccgcgc cggtgcgctg
     1261 ccggtggcgg gcgatcttga cgagcggcgc agcctcggtc gcctcggcgg cctcgccgac
     1321 atcgtcctgc acctggcgcc gccgccggca agcggtcatc tcgactcacg gacgcagcac
     1381 ctgctggcgg cactggaaag cgcgaaaagt ctaccacgtc gtctgatcta cgtaagcact
     1441 acgggcgttt acggcgattg cgccggcgcc tggatcgacg agaccagggc ttgctcgccg
     1501 ggcaacgcgc gggcgcagcg ccgggtcgat gccgaacgcc agttgcgggc ctggggggcg
     1561 cggcgcggcg tcgccgtcag catc
//
LOCUS       DAOSDX010000765         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26056_length_1584_cov_2.158927, whole genome shotgun sequence.
ACCESSION   DAOSDX010000765 DAOSDX010000000
VERSION     DAOSDX010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26056_length_1584_cov_2.158927"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PLN02_14065"
     CDS             complement(<1..1280)
                     /locus_tag="PLN02_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOI53502.1"
                     /translation="MDIVILDGCAASHPVSQQLRQLVAGRALSVSHFALAGINLAPCT
                     GDFDCWTRTPGICRTRDAAQEIACAMRNARLVIFLSPVVCGGYSADLKKAVDRLIGLV
                     HPFFTKRDGLTRHRPRYRDYPPMLFLGLSDRSDDATSTTFREYAGGNAINLLASRYVC
                     RIVSPQADDCPAQLAAALDSALAADVGDVFPTPAADALAAVCRADPGPPLAAPATAAI
                     FIGSARPKGGSTSEALARGLAAALEDKGTRCTLLHAAAFIKPGAAAERALATMLAADL
                     LVVSAPLYVDGLPYLTTRALERLGRQLAALDVGPRRLVGILNCGYPEAVHNRSALRQL
                     RHFAEQHGLAWGGGLALGAGEIIHGRPIDDHPLLLRRPIRALRLAGAALGAGLPVPKP
                     AIHLMARLLVPAGLFRRLAAIRWVRDARANHLPRA"
     gene            complement(1359..>1584)
                     /locus_tag="PLN02_14070"
     CDS             complement(1359..>1584)
                     /locus_tag="PLN02_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53503.1"
                     /translation="VDGNIGLLGADYPGYFPCGDSAALAECLRELRRSQGAADGLLVR
                     LRAAVAARAELFQPSREQAALRELVHQLAA"
BASE COUNT          278 a          525 c          571 g          210 t
ORIGIN      
        1 gcgcgcggca agtgattggc gcgcgcatcc ctgacccagc ggatagccgc cagccggcgg
       61 aacagaccgg ccggcaccaa cagccgggcc atcagatgga tcgccggttt gggtaccggc
      121 agcccggcgc cgagcgcggc accggccagg cgtaaggcgc ggatcggccg gcgcagcagc
      181 agcgggtgat cgtcgatcgg ccgaccatgg atgatttcgc cggcaccgag cgccagtccc
      241 ccgccccaag ccagtccgtg ctgttcggca aaatgccgta actggcgcag cgcgctgcgg
      301 ttatggacag cttccgggta gccgcagttg aggatgccga ccagacggcg cggcccaacg
      361 tcaagcgccg cgagttgccg accgaggcgt tcgagggcgc gggtcgtcag gtagggcaaa
      421 ccatcgacgt agagcggcgc tgagacgacc agcagatcgg ccgccagcat tgttgccagc
      481 gcccgctcgg cggccgcacc gggcttgatg aaggcggctg cgtgcagcag cgtgcaacgc
      541 gttcccttat cctccagcgc cgccgccagc ccgcgcgcca gcgcttccga ggtgctgccg
      601 cccttgggtc gggcgctgcc gatgaaaatc gccgccgttg ccggcgccgc cagcggcggc
      661 ccgggatcgg cacggcagac tgcggcgagc gcgtcggcgg caggggtcgg gaagacgtca
      721 ccgacatcgg cggccagcgc gctgtccagt gcggcggcga gttgcgccgg acagtcgtcg
      781 gcctgcggcg agacgatgcg gcagacatag cgcgaagcca acaggttgat cgcgttaccg
      841 ccggcgtact cgcggaaagt cgtcgaggtc gcgtcgtcgc tgcggtcgct gaggccgagg
      901 aaaagcatcg gcgggtaatc ccggtagcgc ggccgatgcc gggtcaggcc gtcgcgcttg
      961 gtgaagaagg gatgcaccag gccgatcagg cggtcgacgg cctttttcag atcggcgctg
     1021 taaccgccac agacgaccgg cgacaggaag atgaccaggc gggcgttgcg catggcgcag
     1081 gcgatctcct gcgccgcatc gcgggtgcgg caaatgcccg gggtgcgcgt ccagcaatcg
     1141 aaatcgccgg tgcatggcgc cagattgatg ccggccaggg cgaagtggct gaccgaaaga
     1201 gcgcgcccgg cgaccagttg ccgcagttgc tggctgaccg gatgactggc ggcgcaacca
     1261 tcaagaatga cgatatccat gggcggcggc gaaagaacat aacgccagca ttctttcacc
     1321 ggcgaccgtc gatgtatacc ccgggcatac cccaggcatc aggcggcgag ttggtgtacc
     1381 agttcgcgca aggccgcctg ctcgcgcgac ggctggaaaa gttcggcgcg tgccgccacc
     1441 gcggcccgca gccggaccag caggccgtcg gcagcaccct ggctgcgccg cagttcgcgc
     1501 aggcattcag ccagcgcggc gctgtcgccg caggggaaat agcccggata atcggcaccg
     1561 agcaggccaa tgttaccgtc cacc
//
LOCUS       DAOSDX010000766         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26078_length_1582_cov_3.368697, whole genome shotgun sequence.
ACCESSION   DAOSDX010000766 DAOSDX010000000
VERSION     DAOSDX010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26078_length_1582_cov_3.368697"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1542)
                     /locus_tag="PLN02_14075"
                     /pseudo
     CDS             complement(88..1542)
                     /locus_tag="PLN02_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
BASE COUNT          268 a          389 c          542 g          383 t
ORIGIN      
        1 gtcagcttcg ctgaaatcgt cgcaacgaag cctgtcgccc agttttgtaa tctggctatt
       61 gctgagtgtc atggggttgg cctcgattta agagccttcg atttccgcta cgatggcatc
      121 aatctctttg cgtagctggt cgatccgggc aacggtggtc ttcagttcgg cattgagctg
      181 ggcaatatcc acgacttcgc ggtcgtccct ggcttcgatg tagctgctga ccgacaggtt
      241 gtagtcgttg gcggcaatcg cttcaaagct caccgagtgg gcgaagtgcg cgacatcggc
      301 cttgctgtcg aaggcctgca tgatctcttc gatgtgccgg tcggtcagcg tgttgttgtt
      361 ggtttccttc ttgaacaggc cgctggcgtc gatgaactgg gtttgggtgt cggtcttgtg
      421 cttggacagc accaggatat tgacggcgat ggtggtgccg aagaacaggt tgggggccag
      481 cgaaatcacc gtttcgacgt agttgttgtc gaccaggtat tggcggattt tctgctcggc
      541 gccgccgcgg tagaagatac cggggaagca gacgatggcg gcgcggccct tggcggagag
      601 gtagccgagg gcgtgcagca cgaaggcgaa gtcggccttg gatttgggcg ccagcacgcc
      661 ggccggggcg aagcgttcgt cgttgatcag cgtcgggtcg tcgctgccga cccacttcac
      721 cgaatagggc gggttggaga cgatggcgtc gaagggtttt tcgtcgccga aatgcggttc
      781 ggtcagggtg ttgccgagct ggatgtcgaa tttgtcttag ttgatgttgt gcaggaacat
      841 gttcatccgc gccaggttgt acgtggtgtg gttgatttcc tgcccgtaga agccgtcttc
      901 gatgaggtgg gcgtcaaagt gtttcttggc ttgcagcagc agcgagccgg agccgcaggc
      961 cgggtcgtag atcttgttga cgctggtctg cttgtgcatg gccagttgcg cgatcagttt
     1021 ggagacgttt tgcggcgtga agaattcgcc gccggatttg ccggcgttgg cggcgtagtt
     1081 ggagatgagg aattcgtagg cgtcgccgaa gaggtcgatg cgatggtctt caaaatcatc
     1141 cgagtcgcta aagtccaggt cggccacgcc cttgagcacg gcggccagac gggtgttctt
     1201 gtccttgacg gtattgccga ggcggttgct ggtggtgtcg aaatcggcga acaggccttt
     1261 gatgtcctgt tccgacgggt agccgctggc ggagctttca atcgcggcaa agatggcggc
     1321 gagctcggtg ttcaggcttt cattcgtatt ggcgccagcg acgaccttgg cgaacagctg
     1381 gctggggtag atgaaataac ccttggtctt gacggcgtcg tccttgatgg ccggagtgat
     1441 gacactgtcg ggcagtgcgg catagtcgat gctgtcgtcg ccgcctgcga tgtaggcggc
     1501 gaagttctcg ctgatgaatc ggtagaacaa cgcgccgagc acgtattgct tgaaatccca
     1561 gccgtcgacc gcaccccgga cc
//
LOCUS       DAOSDX010000767         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26132_length_1580_cov_3.059672, whole genome shotgun sequence.
ACCESSION   DAOSDX010000767 DAOSDX010000000
VERSION     DAOSDX010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26132_length_1580_cov_3.059672"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PLN02_14080"
     CDS             complement(<1..1046)
                     /locus_tag="PLN02_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOI53504.1"
                     /translation="MVSQRLTVLQVLPALESGGVERGTLEVADHLVRQGHRSLVMSAG
                     GRLVERLVGEGSEHFAWPIGRKSPLTLRLIGPLRRFLAEQRVDVLHVRSRLPAWICRL
                     AWQGMPASTRPRLVTTVHGYYSVGAYSAVMTRGEAVIAVSSSIADYLRANYPACDPAT
                     VRVIPRGIDPALYRPAYRPSPQWLDDWRAVWPQFVGKRLLVLAGRITRLKGHHDFIAL
                     IAALKASHPDIHGVVVGEADARHLPYLAELQAAAKAQGFADHLTFTGHRGDLREIFSV
                     SALVLSLSTQPEAFGRTVLEALALGVPVVAYAHGGVAELLAAMFPAGRVAPGERDELI
                     ETCRRVLAGDHRPAP"
     gene            complement(1028..>1580)
                     /locus_tag="PLN02_14085"
     CDS             complement(1028..>1580)
                     /locus_tag="PLN02_14085"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOI53505.1"
                     /translation="TQGALNTMQRSVAADPNVGLILLGGISRHYVWQDDEILAAVGKL
                     VAATPQVHWTLTTSPRTPASLLDGLQGLAHANLEVVPFARTTQGWLHEVLGCAGQVWV
                     TPDSVSMVYEALTSGTGVGVFDLPTRHSSRVVRGLERLVQSGWVTRFQNWQAGQSLPR
                     LNGQLDEAARCAKWIKQFWYPNA"
BASE COUNT          265 a          546 c          562 g          207 t
ORIGIN      
        1 ggcgccggac ggtgatcgcc ggcaagaacg cgccggcagg tttcgatcag ttcgtcgcgc
       61 tcgcccgggg ccacccggcc ggccggaaac atcgccgcga gcagttcagc gacgccgcca
      121 tgggcgtagg cgacgaccgg cacgccgagc gccagcgctt ccagcaccgt acgcccgaag
      181 gcttccggct gcgtggacag cgacagcacc agcgcgctga cgctgaagat ctcgcgcagg
      241 tcgccacggt ggccggtgaa cgtgaggtga tcggcaaaac cctgcgcctt cgccgccgcc
      301 tgcagctcgg ccaggtaagg cagatggcgg gcgtcggcct cgccgacgac gacgccgtgg
      361 atgtccgggt ggctggcctt gagcgcggcg atcagggcga tgaagtcgtg gtgccccttg
      421 agccgggtga tgcgcccggc aagcacgagc aggcgtttgc caacgaactg cggccagacc
      481 gcccgccagt cgtccagcca ctgcggcgac ggccggtaag ccgggcgata gagcgccgga
      541 tcgatgccgc gcgggatgac gcgcacggtc gccggatcgc aggcgggata gttagcgcgc
      601 aggtagtcgg cgatcgaact ggaaaccgcg atcaccgcct cgccgcgcgt catcaccgcg
      661 ctataggcgc cgaccgagta atagccatgc accgtcgtca ccaggcgcgg ccgcgtcgag
      721 gccggcatgc cctgccaggc cagccggcag atccaggccg gcagacggga acgcacgtgc
      781 agcacgtcca ccctctgttc ggcgaggaag cggcgcagcg gaccgatcag gcgcagggtg
      841 agcggcgact tgcggccgat cggccaggcg aaatgttcgg agccctcgcc gaccagccgc
      901 tcgaccaggc gcccgccggc cgacatgacc agcgaacgat ggccctggcg gaccaggtgg
      961 tccgccactt ccagcgtgcc gcgttcgacg ccgccgcttt ccagcgccgg caggacttgc
     1021 aggacggtca ggcgttggga taccaaaatt gcttgatcca cttcgcgcaa cgcgcggctt
     1081 cgtcgagttg gccgttcagc cggggcagcg actggccggc ctgccagttc tggaagcgcg
     1141 tcacccaacc gctctgcacc agccgttcga gaccgcgcac gacgcggctg gaatggcgcg
     1201 tcggcaagtc gaaaacgccg acgccagtgc ccgaggtcag cgcctcatag accatcgaca
     1261 cgctgtccgg cgttacccag acctggccgg cgcagcccag cacctcgtgc agccagcctt
     1321 gcgtggtccg cgcgaagggg acgacctcga gattggcatg cgccagcccc tgcaggccgt
     1381 cgagcagcga ggccggcgtg cgcggcgagg tggtcagcgt ccaatggacc tgcggcgtgg
     1441 cggcgaccag cttgccgacc gccgccagga tttcatcgtc ctgccagacg tagtggcgcg
     1501 aaatgccgcc gagcaggatc aggccaacgt tggggtcggc ggcgaccgag cgctgcatgg
     1561 tgttgagcgc gccctgggtg
//
LOCUS       DAOSDX010000768         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26150_length_1579_cov_3.250656, whole genome shotgun sequence.
ACCESSION   DAOSDX010000768 DAOSDX010000000
VERSION     DAOSDX010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26150_length_1579_cov_3.250656"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /gene="hisS"
                     /locus_tag="PLN02_14090"
     CDS             complement(<1..809)
                     /gene="hisS"
                     /locus_tag="PLN02_14090"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOI53506.1"
                     /translation="MSQTLQAVRGMNDILPEEAAFWELFEDTVRTWLKGYGYKPIRMP
                     IVEPTPLFKRAIGEVTDIVEKEMYSFIDGLNGEALTLRPEGTAGCVRAVIEHNLAARQ
                     TQRLYYLGQMFRHERPQKGRYRQFHQVGVETFGIAGPDIDAELILMGARLWADLGLDG
                     IELQINSLGQPAERAEHRAALIAYLEQHVEVLDEDAKRRLYTNPLRILDTKNPALQDI
                     CAAAPKLIDFLGAESLAHFEGVQQVLRDAGQPFTINPRLVRGLDYYNLTVF"
     gene            complement(806..>1579)
                     /locus_tag="PLN02_14095"
     CDS             complement(806..>1579)
                     /locus_tag="PLN02_14095"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOI53507.1"
                     /translation="ELLARIMDENSKRAEPLDATEMMRHAMVTSALESAAKAEEVGLA
                     GEKIILSCKVSSVQDLIAIYRELARRADYALHLGLTEAGMGSKGIVASTAALSVLLQE
                     GIGDTIRVSLTPEPGGSRSQEVIVAQEILQTMGLRAFTPMVAACPGCGRTTSTFFQEL
                     AGEVQDFVRARMPEWKLHYDGAENMTLAVMGCIVNGPGESKHANIGISLPGTGEAPSA
                     PVYEDGEKTVTLKGDNIAREFKDIIERYVQRTYKKKLQA"
BASE COUNT          249 a          510 c          517 g          303 t
ORIGIN      
        1 tcgaacacgg tgaggttgta gtagtcgagc ccgcgcacca ggcgcgggtt gatggtgaac
       61 ggctggccgg cgtcgcgcag cacctgctgc acgccctcga aatgcgccag cgattcggcg
      121 ccgaggaagt cgatcagctt cggcgccgcg gcgcagatgt cctgcagcgc cgggttcttg
      181 gtgtcgagga tacgcagcgg gttggtgtac agacgacgct tggcgtcctc gtcgaggacc
      241 tcgacgtgct gttccaggta ggcgatcagc gcggcacgat gctcggcgcg ctcggccggc
      301 tgaccgaggc tgttgatctg cagttcgatg ccatcgaggc cgaggtcggc ccacaggcgg
      361 gcgcccatca ggatcaattc ggcatcgatg tccggaccgg cgatgccgaa agtttcgacg
      421 ccgacctggt ggaactgccg gtaacgcccc ttctgcggcc gctcgtggcg gaacatctgg
      481 ccgaggtagt agaggcgctg cgtctgccgc gccgccagat tgtgctcgat gacggcgcgc
      541 acgcagccgg cggtgccttc cggacgcagg gtcagcgctt cgccgttgag gccgtcgatg
      601 aaggaataca tttccttctc gacgatgtcg gtgacttcgc cgatggcgcg cttgaacagc
      661 ggcgtcggct cgacgatggg catgcggatc ggcttgtaac catagccttt cagccaggtg
      721 cggacggtgt cctcgaacag ttcccagaac gccgcttctt ccggcaggat gtcgttcatc
      781 ccgcgcacgg cttgcaaagt ctgactcatg cttgcagttt cttcttgtag gtgcgctgga
      841 cgtagcgctc gatgatgtcc ttgaattccc tggcgatatt gtcacccttc agggtcacgg
      901 tcttctcgcc gtcctcgtag accggcgccg acggcgcttc accggtgccc ggcagcgaga
      961 tgccgatgtt ggcgtgcttc gattcgcccg gaccattgac gatgcagccc atgacggcca
     1021 gcgtcatgtt ctcggcgccg tcgtaatgca gcttccattc cggcatgcgg gcacgcacga
     1081 agtcctgcac ctcgccggcc agttcctgga agaaggtcga ggtcgtccgg ccgcagcccg
     1141 ggcaagccgc gaccatcggc gtgaacgcgc gcaggcccat ggtctgcagg atttcctggg
     1201 cgacgatgac ttcctggctg cgcgaaccac ccggctccgg cgtcagcgag acgcggatgg
     1261 tgtcgccgat gccttcctgg agcagcaccg acaaagcggc ggtcgacgca acgatgccct
     1321 tggagcccat gccggcctcg gtcaagccca gatgcagcgc gtaatcggcg cgccgcgcca
     1381 gttcgcggta gatcgcgatc aggtcctgga cgctcgacac cttgcacgag aggatgatct
     1441 tctcgccggc caggccgact tcctcggcct tcgccgccga ttcgagcgcc gacgtcacca
     1501 tcgcgtggcg catcatctcg gtcgcatcga gcggctcggc acgcttgctg ttctcgtcca
     1561 tgatccgcgc cagtaattc
//
LOCUS       DAOSDX010000769         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26255_length_1574_cov_3.578670, whole genome shotgun sequence.
ACCESSION   DAOSDX010000769 DAOSDX010000000
VERSION     DAOSDX010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26255_length_1574_cov_3.578670"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="PLN02_14100"
     CDS             <1..1530
                     /locus_tag="PLN02_14100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI53508.1"
                     /translation="VWEATEIGGQPYLRYLRAMYMEDGCEKCHAILGYKKGDMRGATG
                     LNLPLAPYLERVGESRWQLGLSHALFWLLGLAGIAFGARMSARWEQERAAVQRELVQH
                     RDRLEEQVRERTAALFVAKEAAESASRAKSAFLANMSHELRTPMNGIMGMLELSLKRS
                     SDPKVREQLQKARLSSKMLLAVINDILDLSKIEAERLVLESIEFRLGEVLENVTSLMA
                     ERVAEKGLQLRVDMADGLAGQAFRGDPLRLGQVLLNLTSNAVKFTNSGSVTIHVHPLA
                     DDAERMQLRFAVEDTGIGIAPAEQQRLFEAFEQADSSMTRKYGGSGLGLAICKQLVRL
                     MGGEVEIESELGQGSTFRFSVWLAKSAAHTAPNGLAAGTAEEAALWRDFAGVRVLLAE
                     DEPINQEVSRCLLEDAGFVVDLAADGRIAVELADRRPYGLILMDMQMPNMNGLDASRA
                     IRKGEMNRHTPIVAMTANAFDEDRKTCLDAGMDDHVSKPVDPERLFLTLAHWLTRSCR
                     E"
BASE COUNT          286 a          513 c          524 g          251 t
ORIGIN      
        1 gtctgggaag ccaccgagat cggcggccag ccctatctgc gctacctgcg cgccatgtac
       61 atggaagatg gctgcgagaa atgccacgcc atcctgggtt acaagaaggg cgacatgcgc
      121 ggtgcgaccg gcctcaacct gccgctggcg ccctatctgg agcgggtcgg cgagtcgcgc
      181 tggcaactcg gcctcagtca cgccctgttc tggctgctcg gcctcgccgg cattgccttc
      241 ggcgcccgga tgtcggcgcg ctgggagcag gaacgggcag ccgtccagcg cgagctggtg
      301 cagcaccgcg accgcctgga agagcaggtc cgcgagcgga cggctgcact gttcgtggcc
      361 aaggaagcgg ccgagtcggc gagccgggcg aagagcgcct tcctcgccaa catgagccac
      421 gagttgcgca cgccaatgaa cggcatcatg ggcatgcttg aactctcgct gaagcgcagc
      481 agcgatccca aggtccgcga acaactgcag aaggccaggc tatcgtcgaa gatgctgctc
      541 gccgtgatca acgacatcct cgacctgtcg aagatcgagg ccgagcgcct ggtgctggag
      601 agcatcgagt tcaggctcgg cgaagtcctt gagaacgtca ccagcctgat ggccgagcgg
      661 gtcgccgaaa agggcctgca gttgcgcgtc gatatggccg acgggctcgc cggccaggcc
      721 tttcggggcg atcccttgcg cctgggccag gtgctgctca acctgaccag caacgccgtc
      781 aaattcacca acagcggcag cgtcaccatt cacgttcatc cgcttgccga tgatgccgag
      841 cggatgcagt tgcgcttcgc ggtcgaagat accggcatcg gcattgctcc ggccgagcag
      901 caacgcctgt tcgaagcctt cgagcaggcc gacagctcaa tgacccgcaa gtacggcggc
      961 tccggcctcg ggctggcgat ctgcaagcaa ctggtacgcc tgatgggcgg cgaggtcgaa
     1021 atcgagagcg agctgggtca gggcagtacc ttccgttttt cggtctggct ggcaaagtcc
     1081 gccgcgcaca cagcgccgaa cggcctggcg gccggtacgg cggaagaggc cgcgctctgg
     1141 cgggatttcg ccggcgtgcg ggtgctgctg gcggaggatg aaccgatcaa ccaggaagtg
     1201 tcgcgctgcc tgctcgagga cgccggcttc gtcgtcgatc ttgccgccga tgggcggatc
     1261 gccgtcgaac tggccgatcg tcgtccctac ggactgatcc tgatggacat gcagatgccc
     1321 aacatgaacg gcctcgacgc ctcccgggcc atccgcaagg gcgagatgaa ccggcatacg
     1381 ccgatcgtcg cgatgaccgc caacgccttc gacgaagacc gcaagacctg cctcgacgcc
     1441 ggcatggacg accacgtcag caaaccggtc gaccccgagc gcctgttcct caccctggcc
     1501 cactggctga cgcgcagttg ccgggagtag ggggcgcagg cgggtggcct caagcttgcc
     1561 actgatcttg gcct
//
LOCUS       DAOSDX010000770         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26266_length_1574_cov_2.510862, whole genome shotgun sequence.
ACCESSION   DAOSDX010000770 DAOSDX010000000
VERSION     DAOSDX010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26266_length_1574_cov_2.510862"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..714)
                     /locus_tag="PLN02_14105"
     CDS             complement(37..714)
                     /locus_tag="PLN02_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOI53509.1"
                     /translation="MANDAAGRLPDTVLAAWQHEVAAAELSPVLPDGCQDLICRIDAG
                     QQPHWFVSPLATTAEQVASPGGSRYLGFRLHPGTRIDTSGLLAAVASRTPDAYREIGN
                     LLADFTRRDPATQEILATLAEVATPAAARQRLGIRERTLERLCRRDTGQTAGFWIALA
                     RVRRAAVAIDSELPLTDIAGLCGYADQAHLNRAFRRWFAASPAQFRCSPQLLSLARAS
                     GFATAPI"
     gene            complement(704..>1574)
                     /gene="hemW"
                     /locus_tag="PLN02_14110"
     CDS             complement(704..>1574)
                     /gene="hemW"
                     /locus_tag="PLN02_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOI53510.1"
                     /translation="AEITLEANPGTVEAGKFARFRAAGVNRLSLGIQSFNDEHLKALG
                     RIHGSAEAKRAAQLAGEHFERFNLDLMYGLPYQTQEQALADIDTALSFAPPHLSCYQL
                     TLEPNTHFAAFPPDLPEGDLCADMQEAIEARLAAAGYANYETSAFAQPGRQCLHNLNY
                     WQFGDYLGIGAGAHSKLTLHDRVLRQMRHKHPKAYLENVRAGRPIQEEHAVAAAELPF
                     EFMMNALRLADGFAPSLFELRTSRPLAAILPQLKAAQADGLLDIGPEKIAPTAKGRRF
                     LNVLLERFLDDGQ"
BASE COUNT          215 a          450 c          639 g          270 t
ORIGIN      
        1 gggctacctc gtcgaactgt gtacggcggt cggcgcttag atcggggcgg tggcgaaacc
       61 gctggcgcgg gccagcgaca ggagttgcgg cgagcagcgg aattgcgcgg gtgaggcggc
      121 aaaccagcgg cggaaggcac ggttcaggtg ggcctggtcg gcatagccgc agaggccggc
      181 gatgtcggtt agcggcagtt cgctgtcgat ggcgacggcg gcgcggcgga cgcgcgctag
      241 tgcaatccag aagccggcgg tttgcccggt gtcgcgccgg cacagtcttt ccagcgtgcg
      301 ttcgcggatg cccaggcgct gccgcgcggc ggccggcgtg gcgacttcgg ccagggtggc
      361 gagaatctcc tgcgttgccg ggtcgcggcg ggtgaagtcg gcgagcagat tgccgatttc
      421 gcggtacgcg tcgggcgtcc gggaggcgac tgcagcgagc aggccgcttg tgtcgatgcg
      481 ggtgccgggg tgcaggcgaa aacccaggta acggctgccg cccgggctgg cgacctgttc
      541 ggcggtggtg gccaaggggc tgacgaacca gtgtggctgt tgcccggcgt cgatccggca
      601 gatcaggtcc tggcagccgt cgggcagcac cggcgacagt tccgccgccg cgacttcgtg
      661 ctgccaggcg gcgaggacgg tgtcgggcag gcggccggcc gcgtcattgg ccatcgtcga
      721 ggaagcgttc cagcaagacg ttgaggaagc gccggccctt ggcggtcggc gcgatttttt
      781 ccgggccgat gtcgagcagg ccgtcggcct gcgccgcctt gagttgcggc aggatagcgg
      841 cgagcggtcg cgaggtgcgc agttcgaaca ggctgggggc gaagccgtcg gccaggcgca
      901 gtgcgttcat catgaattcg aagggcagtt cggcggcggc gaccgcgtgt tcttcctgaa
      961 tcggtcggcc ggcgcggacg ttttccaggt aggccttggg gtgcttgtgg cgcatctggc
     1021 gcagcacgcg gtcgtgcagc gtcagcttgg aatgggcgcc ggcgccgatg ccgaggtagt
     1081 cgccgaactg ccagtagttg aggttgtgca ggcactgccg gccgggctgg gcgaaggccg
     1141 aggtttcgta gttggcgtag ccggcggcgg ccagtcgggc ttcgatggct tcctgcatgt
     1201 cggcgcacag gtcgccttcg ggcaggtcgg gcgggaaggc ggcgaagtgc gtgttcggct
     1261 ccagcgtcag ctggtagcag gagagatgcg gcggcgcgaa ggacagcgcg gtgtcgatgt
     1321 cggcgagcgc ttgctcctgc gtttgataag gcaggccgta catcaggtcg aggttgaagc
     1381 gctcgaagtg ctcgccggcc agttgcgccg cgcgcttcgc ttcggcgctg ccgtggatgc
     1441 ggccgagcgc cttgaggtgc tcgtcgttga agctctggat gccgagcgac agccgattga
     1501 ccccggcggc gcgaaagcgg gcgaacttgc cggcctcgac cgtgccggga ttggcttcga
     1561 gtgtgatttc ggct
//
LOCUS       DAOSDX010000771         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26287_length_1573_cov_2.655468, whole genome shotgun sequence.
ACCESSION   DAOSDX010000771 DAOSDX010000000
VERSION     DAOSDX010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26287_length_1573_cov_2.655468"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1573)
                     /locus_tag="PLN02_14115"
     CDS             complement(<1..>1573)
                     /locus_tag="PLN02_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI53511.1"
                     /translation="ELKRIKGTRDVSTLGGPDHAVRVLLDAERMNAFGVTPQDIAGAL
                     TVSNDLQSSGSLIAGNRDILVQTGTYLESAADVRRLVVAVVDRKPIYLGDVARVEDGP
                     DQPKRYAWFGSSAGEFPAVTIQLSKQPGVNAADIADAAIARIDSLKNVVIPDGVEVTV
                     TRNYGQTATEKAQKLIGKLVFATAFVVALVFFALGRREAVIVGVAVTLTLAATLFASW
                     AWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRWQALHPDKGLLEIIPGAVDEVGGP
                     TILATLTVIAALLPMAFVSGLMGPYMSPIPINSSMGMALSLAIAFVVTPWLAARLMKA
                     HGHTASSHAPSKLDATLDRTFRRLLTPFLDPAKGAAARLKLGLAVVLLIAGSVSLTYF
                     QLVVLKMLPFDNKSEFQVVVDMPAGTPVEETARVLQEIGGELAKVPEVVNYQAYAGTA
                     SPINFNGLVRQYYLRAAPEKGDIQVNLVDKHERSRQSHEIAVSVRDAIEAIGKRNGGV
                     AKVVEVPPGPPVMSP"
BASE COUNT          257 a          508 c          560 g          248 t
ORIGIN      
        1 atcggcgaca tcaccggcgg gccgggcggc acttcgacca ccttggcgac gccgccgttg
       61 cgcttgccga tggcttcgat ggcatcgcgc accgagacgg cgatctcgtg gctctgccgc
      121 gaacgctcat gcttgtcgac caggttgacc tggatgtcgc ccttttccgg cgccgcgcgc
      181 aggtagtact ggcggaccag accgttgaag ttgatcgggc tggcggtgcc ggcataggcc
      241 tggtaattga cgacttccgg caccttggcc agttcgccgc cgatctcctg cagcacccgt
      301 gccgtttcct cgaccggcgt gccggccggc atgtcgacga cgacctggaa ttcgctcttg
      361 ttgtcgaagg gcagcatctt caggacgacc agctggaaat aggtgagcga caccgagccg
      421 gcaatcagca ggacgacggc cagcccgagc ttgaggcgcg ccgcggcgcc cttcgccgga
      481 tcgaggaagg gcgtcagcag gcggcggaaa gtgcggtcca gcgtcgcgtc gagcttcgac
      541 ggggcgtggc tgctggcggt gtggccgtgc gccttcatca gccgcgccgc cagccagggc
      601 gtgacgacga aggcgatggc cagcgacagc gccatgccca tcgacgagtt gatcgggatc
      661 gggctcatgt acggccccat caggccggag acgaaggcca tcggcagcag cgcggcaatc
      721 accgtcaagg tcgccaggat ggtcggcccg ccgacttcat cgacggcgcc gggaatgatc
      781 tccagcagcc ccttgtccgg gtgcagcgcc tgccagcggt ggatgttctc gacgacgacg
      841 atggcgtcgt cgaccagaat gccgatcgag aagatcagcg cgaacagcga tacccggttc
      901 agcgtgaagc cccaggccca ggaggcgaac agcgtcgccg ccagggtcag cgtcacggcg
      961 acgccgacga tcaccgcctc gcgccggccg agggcgaaga agaccagggc gacgacgaag
     1021 gcggtggcga agaccagctt gccgatcagc ttctgcgcct tctcggtcgc cgtctggccg
     1081 tagttgcggg tgaccgtgac ctcgacgccg tccgggatga cgacgttctt cagactgtcg
     1141 atgcgggcga tcgccgcgtc ggcgatgtcg gcggcgttga cgccgggctg cttggaaagc
     1201 tggatggtga ccgccgggaa ttcgccggcg ctgctgccga accaggcgta gcgtttcggc
     1261 tgatcaggac cgtcctcgac gcgggcgaca tcgccgaggt agatcggctt gcggtcgacg
     1321 acggcaacca ccaggcgccg cacgtcggcc gccgattcga ggtaggtgcc ggtctgcacc
     1381 aggatgtcgc ggttgccggc gatcaggctg cccgacgact gcaggtcgtt ggacacggtg
     1441 agcgcgccgg cgatgtcctg cggcgtgacg ccgaaggcat tcatgcgctc ggcgtcgagc
     1501 aggacgcgca cggcatggtc gggaccgccg agggtggaca cgtcgcgcgt gcccttgatc
     1561 cgcttcagct cga
//
LOCUS       DAOSDX010000772         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26322_length_1571_cov_3.585092, whole genome shotgun sequence.
ACCESSION   DAOSDX010000772 DAOSDX010000000
VERSION     DAOSDX010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26322_length_1571_cov_3.585092"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLN02_14120"
     CDS             complement(<1..231)
                     /locus_tag="PLN02_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53512.1"
                     /translation="MLALQENQERPLAVSDSPQNPLRLYHARTKHAFDAYAEGPGQLD
                     WDAQPAPFRHYPGAPALELPLAADRFERAYGRL"
     gene            complement(437..1420)
                     /locus_tag="PLN02_14125"
     CDS             complement(437..1420)
                     /locus_tag="PLN02_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53513.1"
                     /translation="MAIIRCNKCALLAEHPDEVVGKNVACPKCGAPTPVYPTLFFIEK
                     LLDKYFAAQREVIRLTPGTATTPPPPAPASEIAGIDLANTDFLASELQHGPIYDWFRK
                     KQIKVQANTRGVDTSGFFDEVAEAIGANLPVLKEVLERIRWSQQKEHASATINLDRKS
                     PEEAKAISTFCQQLYDFSFVAKCFHNKPENNVRLILQTAPTIRNFFNGEWLEWHALMS
                     CLRYAKERQRRFSCTRGLELTLSNGDPYEIDVFMLIDGDTPICIECKSGEFRQNIDRY
                     LALKKRLGLAGKQFVMCIAGLSDENAKAFSAMYDLSFVNERGLAGHLSRLF"
BASE COUNT          265 a          440 c          532 g          334 t
ORIGIN      
        1 gagtcggccg taagcgcgtt cgaaacgatc ggcggccagc ggcagttcga gcgccggcgc
       61 ccccgggtaa tggcggaacg gcgccggctg ggcatcccag tcgagttggc cggggccttc
      121 ggcgtaggca tcgaaagcgt gcttggtgcg agcgtggtaa aggcgcagtg ggttttgtgg
      181 gctatccgac acggcgagcg gtctttcctg gttttcttga agggcgagca agggctgtgc
      241 cggtgtagac cggttgttgg ccagaaggct cccggcaacg aaaatgaaac atcgcaagcc
      301 gataattgcc ggcaacaaaa acaagctcac ccgccaaggc ggtttcttgc tgcctgtgcc
      361 atggccccgg ccggtccggg atgcctgctg ccgacgccgc ccgctgggcg gcgcattttt
      421 ttccggggtc tcccgctcag aaaaggcggc tcagatgtcc ggcgagtccc cgttcgttga
      481 cgaaactcag gtcatacatg gccgagaagg ctttggcgtt ttcgtcgctg agtccggcga
      541 tgcacatcac gaactgtttt cccgccaggc ccagacgttt tttcagcgcc aggtagcggt
      601 cgatgttctg gcggaattcg ccgctcttgc attcgatgca gattggcgtg tcaccgtcga
      661 tcagcatgaa aacgtcaatt tcgtacgggt cgccgttgct cagcgtcagt tcgaggccgc
      721 gcgtgcagga aaagcggcgt tggcgctcct tggcgtagcg caggcagctc atcagcgcat
      781 gccattccag ccattcgccg ttgaaaaaat tgcggatggt cggcgccgtc tgcaggatca
      841 gccgaacgtt gttttccggc ttgttgtgga agcacttggc gacgaaggag aaatcgtaga
      901 gctgctggca gaaggtggag atggctttgg cctcttccgg cgacttcctg tccaggttga
      961 tcgtcgcgct ggcgtgctcc ttctgctgcg accagcggat gcgctccagc acttccttga
     1021 gtaccggcag gttggcgccg atggcttcgg ccacttcgtc gaagaagcca ctggtgtcga
     1081 cgccgcgcgt gttggcctgg accttgatct gtttcttgcg gaaccagtcg tagatcggac
     1141 cgtgctgcaa ctcgctggcc aggaagtcgg tattggcgag gtcgatcccg gcgatctcgc
     1201 tggccggcgc ggggggcggt ggtgtcgtgg cggtgcccgg ggtcaggcgg atcacttcgc
     1261 gctgtgcggc aaagtacttg tcgagcagtt tttcgatgaa gaaaagtgtc ggatagaccg
     1321 gtgttggcgc accgcatttc gggcaggcga cgttcttgcc gacaacttca tcaggatgct
     1381 cggcgagcag tgcgcatttg ttgcaccgga tgatggccat gacaataatc cgcggattcg
     1441 agtccgcgga ttataacggg cgagtaacgc cggcggctgt ccgtcagacc cggaacctgg
     1501 ccgtctcggc gctcagattg cgcgcggtgt cgagcaacga ctgggcggcc tgggccgtgg
     1561 tgcgcatgct c
//
LOCUS       DAOSDX010000773         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26326_length_1571_cov_2.819261, whole genome shotgun sequence.
ACCESSION   DAOSDX010000773 DAOSDX010000000
VERSION     DAOSDX010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26326_length_1571_cov_2.819261"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLN02_14130"
     CDS             <1..825
                     /locus_tag="PLN02_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOI53514.1"
                     /translation="QWWYGHNAVGFFLTAGFLGMMYYFVPKQAGRPVCSYRLSIVHFW
                     ALIFTYMWAGPHHLHYTALPDWTQSVGMVFSLILLAPSWGGMINGIMTLSGAWHKLRD
                     DPILKFLVTSLSFYGMSTFEGPMMAIKSVNALSHYTDWTIGHVHSGALGWVAMVSIGS
                     IYFLLPRLFGRREMYAPGLVTVHFWLATIGTVLYIAAMWIAGVMQGLMWRAINADGTL
                     AYSFVEGVKGSYPFWAIRWLGGVFFLSGMLLMAWNMFRTIAAGKVEPVAVIPAAQH"
     gene            834..1013
                     /locus_tag="PLN02_14135"
     CDS             834..1013
                     /locus_tag="PLN02_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HOI53515.1"
                     /translation="MSFDLLLPAYTLLAFFGFLAICAWACDGARDGDFSAAAALPFND
                     PEAEAERRRALLTGR"
     gene            complement(1058..>1571)
                     /locus_tag="PLN02_14140"
     CDS             complement(1058..>1571)
                     /locus_tag="PLN02_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HOI53516.1"
                     /translation="VPALHDDGFQVWDSLAIAEYLAEREPRMWPADARARARARSVCA
                     EMHAGFMNLRRAMPMNLKLRLKGKPATPEVQRDIDRIVEIWEEAREYFAIGEGPWLFG
                     DFSIADAMFAPVACRFTVYNVTLPPVAAAWQETLLDHPAMREWQAAALLEREAHAHYD
                     CLADDYGGAR"
BASE COUNT          216 a          542 c          518 g          295 t
ORIGIN      
        1 cagtggtggt acggccacaa cgcggtcggc ttcttcctga ccgccggctt cctcggcatg
       61 atgtattact tcgtgccgaa acaggcgggt cgcccggtct gttcctaccg tctgtcgatc
      121 gtgcacttct gggcgctgat cttcacctac atgtgggccg gcccgcatca cctgcactac
      181 accgccttgc cggactggac gcagtcggtc ggcatggtct tctcgctgat cctgctggcg
      241 ccgtcgtggg gcggcatgat caacggcatc atgacgctgt cgggcgcctg gcacaaactg
      301 cgcgacgacc cgatcctgaa gttcctggtc acctcgctgt cgttctacgg gatgtccacc
      361 ttcgaagggc cgatgatggc gatcaagtcg gtcaacgcgc tctcgcacta caccgactgg
      421 accatcggcc acgtccattc cggggcgctg ggctgggtgg cgatggtgtc gatcggctcg
      481 atctacttcc tgctgccgcg cctcttcggg cgccgcgaga tgtacgcgcc gggcctggtc
      541 accgtgcatt tctggctggc gaccatcggc accgtgctct acatcgccgc gatgtggatc
      601 gccggcgtca tgcagggctt gatgtggcgc gccatcaacg ccgacggcac gctcgcctac
      661 agcttcgtcg aaggcgtcaa gggctcctac ccgttctggg cgatccgctg gctgggcggt
      721 gtcttcttcc tctccggcat gctgctgatg gcctggaaca tgttccgcac catcgccgcc
      781 ggcaaggtcg agccggtggc ggtgattccg gccgcccagc actgaggacg acgatgagct
      841 tcgacctgct gctgccggcc tacaccctgc tcgccttctt cggcttcctg gcgatctgcg
      901 cctgggcctg cgacggcgcc cgcgacggcg atttctcggc ggccgcggcg ctgcccttca
      961 acgacccgga ggccgaggcg gaaaggcgcc gggcgctgct caccggccgc tgagccggaa
     1021 tgcaaacggg ggccgcggcc cccgtttttc gatgcgctca gcgcgccccg ccgtagtcgt
     1081 cggccaggca gtcgtaatgc gcgtgcgcct cgcgctcgag cagcgccgcc gcctgccatt
     1141 cgcgcatcgc cgggtggtcg agcagggttt cctgccaggc ggcggcgact ggcggcagcg
     1201 tcacgttgta aacggtgaag cgacaggcga ccggcgcgaa catcgcgtcg gcgatcgaga
     1261 aatcgccgaa cagccagggg ccctcgccaa tggcgaagta ctcgcgggcc tcttcccaga
     1321 tttcgacgat gcggtcgatg tcgcgctgca cttccggcgt cgccggcttg cccttgagcc
     1381 gcagcttgag gttcatcggc attgcccggc gcaggttcat gaaacccgcg tgcatttcgg
     1441 cgcagacgct acgcgcccgg gcgcgcgccc gggcgtcggc cggccacatg cgcggttcgc
     1501 gctcggccag gtattcagcg atggccaggc tgtcccagac ctggaagcca tcgtcgtgca
     1561 gcgccgggac g
//
LOCUS       DAOSDX010000774         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26362_length_1569_cov_3.022457, whole genome shotgun sequence.
ACCESSION   DAOSDX010000774 DAOSDX010000000
VERSION     DAOSDX010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26362_length_1569_cov_3.022457"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLN02_14145"
     CDS             complement(<1..456)
                     /locus_tag="PLN02_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOI53517.1"
                     /translation="MIKPEEFAKSGFNFALFFANTTFDGIERLAVLNLAASRSAFEAF
                     VSNVNTLLGVKDFKSFVEVQKELAAPTLEKGIEYSRNVIAIATDAKEKIAKQVETHVA
                     DTSAKVSGMVEKALESAPAGSEVAVAAVKTAMKSANEAMDGLNKVAKQAA"
     gene            complement(688..1254)
                     /locus_tag="PLN02_14150"
     CDS             complement(688..1254)
                     /locus_tag="PLN02_14150"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOI53518.1"
                     /translation="MSFPGRPLAIFLLAFNLVACAPLGGPSGPGERPEMPPGQDAEEG
                     AMPMADPLQEKLARLAAALALTPAQQPLWDDYQETLGALLADQARGSAQRASQSAVQQ
                     LAGKLDVVRNRLSAMEEIQDAASRLYLALDPRQREVADRMLAATVPSLDAGCATPGAR
                     RARGGRPMGGGGPGRGNGGPGQGGGPMW"
     gene            1364..>1569
                     /locus_tag="PLN02_14155"
     CDS             1364..>1569
                     /locus_tag="PLN02_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOI53519.1"
                     /translation="MQIYLAGPDVFQPDALQRAESARRLVSRYGHRALIPLDNEASTA
                     AEIYRANLAQIAAADLVIANLNPFR"
BASE COUNT          275 a          497 c          498 g          299 t
ORIGIN      
        1 ggcagcctgc ttggcaacct tgttcaggcc gtccatcgct tcgttggccg acttcatcgc
       61 ggtcttgacc gcggcgacgg cgacttcaga accggccgga gcggattcca gggccttttc
      121 gaccatgccg gaaaccttgg cgctggtgtc ggcaacatgg gtttcgactt gcttggcgat
      181 cttttccttg gcatcggtgg cgatggcgat cacgttgcgc gagtactcga tgcccttttc
      241 cagcgtcggc gcggccagtt ccttctggac ttcgacgaag gacttgaaat ccttgacgcc
      301 gagcagcgtg ttgacgttgc tgacaaaagc ttcgaaagcg gaacgcgagg cggccaggtt
      361 caggacggcc agacgctcga tgccgtcgaa cgtggtgttg gcgaagaaca gggcgaaatt
      421 gaagccggac ttggcgaact cttcgggttt gatcatgatg aatctcctgg atgggttggg
      481 ggcgtatcgg tcttgaaaca gtcctgcgga actcacacca tcaacatggt gcaatgcagc
      541 atgattcaat tctatccatc gacgaaatgg agtcaagcgc tttttgtgcg ccgcaccatt
      601 tttcgttcaa cagggcagct tcgggtagaa aatgctagcc tgccgaggtt gcaggctgac
      661 gacagcccga tgaccgccgc cccgggatca ccacatcggg ccgccgccct gccccggccc
      721 tccgttgcca cgccccggac cgccaccgcc catcggccgc ccaccccgtg cccgtctcgc
      781 gcccggcgtg gcgcatccgg catcgagcga gggaacggtg gcagcgagca tgcgatcggc
      841 gacttcgcgc tgacgcggat cgagcgccag gtagagtctg gacgcggcgt cctggatttc
      901 ctccatggcc gacagccgat tgcgtaccac gtcaagcttg ccggcaagct gctgcacggc
      961 cgactgactg gcccgctggg cgctgccgcg agcctgatcg gcgagcaagg cgccgagcgt
     1021 ttcctgatag tcgtcccaaa gcggctgttg ggccggggtc agggcgagcg ccgccgccag
     1081 ccgcgccagt ttctcctgga gcggatcggc catcggcatc gcgccttctt ccgcatcctg
     1141 ccccggtggc atctccggcc gctcccccgg cccggaaggg ccgcccagcg gggcgcaggc
     1201 gacgaggttg aaggcgagga ggaagattgc gagggggcgt ccgggaaagc tcatggttgc
     1261 tcatcgttgt tgtcgataac ggcgaatttt ccgcgcagcc tgggttaatc cgggtaagcc
     1321 ggggtaaatt gatggctatc caggaaaaat caggtcattc gccatgcaga tctatctcgc
     1381 cggtcccgat gtcttccagc ccgacgcctt gcagcgcgcc gaatcagcgc gccgtctggt
     1441 gagccgttac ggacatcggg cattgattcc gctggacaac gaagcgagca cggcagcgga
     1501 gatctaccgc gccaacctgg cccagattgc cgccgccgac ctggtgatcg ccaacctcaa
     1561 ccccttccg
//
LOCUS       DAOSDX010000775         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26389_length_1568_cov_2.737607, whole genome shotgun sequence.
ACCESSION   DAOSDX010000775 DAOSDX010000000
VERSION     DAOSDX010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26389_length_1568_cov_2.737607"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1550
                     /locus_tag="PLN02_14160"
     CDS             177..1550
                     /locus_tag="PLN02_14160"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOI53520.1"
                     /translation="MASVRDLIVFAYLAGTENRFVPAGRLTLTETPSPNPAARELASA
                     FAYGTRYLERPDSFELDPVSLSLLDRAAVRGQLLYPVNVLPEFGGLRDAAPDAWGRRV
                     IEARRRVPANTLAEADYLLEAGGDRVGALDVREVLDAPPRPSASDLRSLEYLLQAAEA
                     VEAGLPIPAQLMDILGAGSSAGGARPKATVRDDEGGLWLAKFPARNDTFDVARAEACT
                     LELARNCGLTVPEVRHCDIGQRSVMLIRRFDRFWAPAGQYPAAGPLQETRPGRQLIES
                     RLPFVSGLTLVGCSEYESPDKSYGDLARAIRRYVHPERIARDNEELFARMVFNIFVSN
                     DDDHLRNHGFIRDPRLGGWQLSPLYDVVPRPGLAFERRLHLHIGAAGKAATLDNALSH
                     ATAFLADRAAAIAIIRRVWGEVRQWKTVFESFGAPGLLIDQLASAFRPLDEIASSELV
                     KEVRRGP"
BASE COUNT          225 a          602 c          482 g          259 t
ORIGIN      
        1 gacccggggc gtaccctggc ccaggtcctg cgctaccttg aggccgtcgc cccggggctc
       61 agcctccttg ccctgctgca ggaagaagac cccgcgctgc gcgcccttgc cgcccgcgaa
      121 accactcgcc gcgcccggcc gctgagcgcg gctgaactca aggacctcga cttctgatgg
      181 cgagcgttcg cgacctcatc gttttcgcct acctggccgg cactgaaaac cgcttcgtgc
      241 cggccggccg gctgacgctg accgaaacgc ccagccccaa cccggcggcg cgcgaactcg
      301 cctcggcctt cgcctatggc acccgctatc tggagcggcc ggacagtttc gagctggacc
      361 ccgtcagcct gagcctcctt gaccgcgctg ccgtgcgcgg tcagctcctc tatcccgtca
      421 acgtcctgcc ggaatttggc ggtctgcgcg acgccgcgcc cgatgcctgg ggtcggcgcg
      481 tcatcgaagc ccggcgcagg gtgccggcca ataccctggc ggaggccgac tatctgctgg
      541 aagccggggg ggatcgcgtc ggcgcgctgg atgttcgtga ggtgctggat gcgccgccgc
      601 gtccgtccgc cagtgacttg cgctcgctgg aatatctgct gcaggccgcc gaagccgtcg
      661 aggccgggct gcccattccg gcccaactga tggacatcct cggcgccggt tcatctgctg
      721 gcggcgcccg gccgaaggcc accgtccgcg acgacgaagg cggcctgtgg ctggccaagt
      781 tccccgcccg caacgatacc ttcgacgtgg cccgcgccga agcctgtacc ctggagctcg
      841 cgcgcaattg cggtctgacc gtgcccgagg tccgccactg cgacatcggc cagcgctcgg
      901 tcatgctcat tcgccgcttc gaccgtttct gggcgccggc cggccagtat ccggctgccg
      961 gcccgctgca agagacccgt cccggccggc agttgatcga gtcacgcctg ccgttcgtca
     1021 gcgggctgac cctggttggc tgctcggaat acgaatcccc cgacaaaagc tatggcgacc
     1081 tggcgcgcgc catccgtcgc tacgtgcacc ccgagcgcat cgcccgcgac aacgaagaac
     1141 tcttcgcccg catggtcttc aacatcttcg tcagcaacga cgacgaccac ttgcgcaacc
     1201 acggcttcat ccgcgacccg cgcctcggcg gctggcaact gagcccgctg tacgacgtcg
     1261 ttccccgccc cgggctggcg ttcgagcgac gcctccatct gcacattggc gccgcaggca
     1321 aggccgccac cctcgacaac gcgctcagcc acgccaccgc cttcctggcc gaccgcgccg
     1381 cagccatcgc catcatccgc cgcgtctggg gcgaagtccg ccaatggaaa accgtcttcg
     1441 agtccttcgg cgcccccggt ctgctgatcg accagctggc ttcagccttc cgccccctgg
     1501 acgagatcgc cagttcggag ctggtcaagg aagtgcggcg cgggccttag ctcgcctcat
     1561 ctatcgtt
//
LOCUS       DAOSDX010000776         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26411_length_1567_cov_3.073413, whole genome shotgun sequence.
ACCESSION   DAOSDX010000776 DAOSDX010000000
VERSION     DAOSDX010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26411_length_1567_cov_3.073413"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLN02_14165"
     CDS             complement(<1..771)
                     /locus_tag="PLN02_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOI53521.1"
                     /translation="MQHFDLIIVGGGLAGASLALALRDSALRVALVEAQPPRRPDGWD
                     ARIYAVSPANVAFLDAIGAWRHLDRQRIAPIAAMKVFGDAGGALEFSAYETGVADLGC
                     ILESSLVACELWEGLKRQSNLALFIGQKPAALEFRDEVAVLRLADGDSLSAALLVGAD
                     GRDSWVREAAGLPAHNRHYGEKGVVANFATDRPHRNIAWQWFGDDGVLAYLPLPGQRM
                     SIVWSAPDALADELCRLSPEALCERVEQAGHGVLGKLDL"
     gene            complement(802..1197)
                     /locus_tag="PLN02_14170"
     CDS             complement(802..1197)
                     /locus_tag="PLN02_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53522.1"
                     /translation="MNNPNSDTQIEETEPAVDAELPETVVEEQPPEFQPLDLKRRLRE
                     LLSVPERDRSDEQWDEIIELEIQLAPGNRISGSPVPGGGGGQPRHNAPRQNKPGNAAA
                     PAAQKKHRPRSKNNRRPRQGGKPGGGEAV"
     gene            complement(1256..1552)
                     /locus_tag="PLN02_14175"
     CDS             complement(1256..1552)
                     /locus_tag="PLN02_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOI53523.1"
                     /translation="MNKSELVEVAAQEAGITKAAADKALSAIVGAVVQAVAKGESVTL
                     VGFGTFKSAPRAARTGKNPKTGATLKIPATVVPKFTAGTAFKAAVAPKKGGKKK"
BASE COUNT          284 a          532 c          472 g          279 t
ORIGIN      
        1 cagatcaagc ttgccgagaa cgccgtgccc ggcttgttcg acgcgctcgc acagagcttc
       61 gggcgagagc cggcacaact cgtcggccag cgcatccggc gccgaccaga cgatcgacat
      121 gcgctgcccg ggcaagggca ggtaagccag caccccgtca tcgccgaacc actgccaggc
      181 gatgttgcgg tgcggccgat cggtcgcgaa gttggcaacg acgcccttct cgccgtagtg
      241 ccggttgtgc gccggcaatc cggccgcttc gcgaacccag gaatcgcgcc cgtcggcgcc
      301 gaccagcagc gccgccgaca ggctatcgcc atcagccagt cgcaacaccg cgacctcatc
      361 gcgaaattcc agcgccgcag gtttctgccc gatgaatagc gccaggttgg actgacgctt
      421 caggccttcc cacaactcgc aagcgaccag cgacgattcg agaatgcacc cgagatcggc
      481 aacccccgtc tcatacgcgg aaaattcaag cgccccgccg gcatcgccaa acaccttcat
      541 cgccgcaatc ggcgcgatgc gctgccgatc gagatggcgc caggcgccga tcgcatcgag
      601 gaaggcgacg ttggccggac tgaccgcgta gatgcgtgca tcccagccat ctggacgacg
      661 cggcggctgc gcctcgacca gggcgacgcg cagcgcactg tcgcgcaggg cgagcgccag
      721 actggcgccg gccagaccgc cgccgacaat gatcagatca aaatgctgca tgcctgcccg
      781 gacaaaaagt ctgggtgtga atcacaccgc ttcgccaccg ccgggcttgc ctccctggcg
      841 cggccgacga ttgttcttcg aacgcggccg atgcttcttc tgcgccgccg gggctgccgc
      901 attgccaggc ttgttctggc gcggtgcgtt atgacgcggc tgcccaccgc caccgcctgg
      961 taccgggctg ccggaaatcc ggttgcccgg cgccagctgg atttccagct cgatgatttc
     1021 atcccactgt tcatcgctgc ggtcacgctc gggaaccgac aacagttcgc gcaagcggcg
     1081 cttcaggtca agcggctgga actcgggcgg ctgctcttcg acgacggtct cgggaagctc
     1141 ggcatcgacg gcaggctcgg tttcttctat ctgggtatcg ctgttcggat tgttcattgc
     1201 aaagtaaaaa agcggagtcc gtcaaagacg gcccccgcat gtggtgagag ctcggttatt
     1261 ttttcttgcc gccttttttc ggggcaacgg ccgccttgaa agccgtaccg gcagtgaact
     1321 tcggcacgac ggtggccgga atcttcaggg tcgcaccggt tttcggattc ttgccggtac
     1381 gggccgcgcg cggcgccgac ttgaacgtac caaatccgac gagcgttacc gactcgccct
     1441 tggcaacagc ctggacaacc gcgccgacga tggccgacaa cgccttgtct gcggccgcct
     1501 tggtaatacc agcttcttgt gcagcaactt cgaccagctc cgatttattc atctaagtgc
     1561 ctcctag
//
LOCUS       DAOSDX010000777         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26438_length_1566_cov_3.404368, whole genome shotgun sequence.
ACCESSION   DAOSDX010000777 DAOSDX010000000
VERSION     DAOSDX010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26438_length_1566_cov_3.404368"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLN02_14180"
     CDS             complement(<1..874)
                     /locus_tag="PLN02_14180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOI53524.1"
                     /translation="MKHVKKSAKLANVCYDIRGPVLQMAKQMEEEGHKIIKLNIGNLA
                     AFGFDSPEEIQQDIIRNLPNAAGYTDSKGIFAARKAIMHYTQQKGIKGVTLEDIYVGN
                     GVSELIVMAMNALLDAGDEVLVPAPDYPLWTAAISLSGGKPVHYLCDEEKGWLPDLDD
                     IRSKINKNTRAIVIINPNNPTGALYPDDLLHEIIAIAREHHLIIYADEVYDKVLYDGV
                     KHTSIAALSDDVLTITFNGLSKNYRSCGYRAGWMVVSGDKRHAKDYIEGLDILASMRL
                     CANAPGQHGIQTALG"
     gene            946..1317
                     /locus_tag="PLN02_14185"
     CDS             946..1317
                     /locus_tag="PLN02_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HOI53525.1"
                     /translation="MKLHITPNAGLNLFTAYGDDYVAVNHEKHQSSLIVLPDRLLAAW
                     TSASVGSLQEADMQTLLGLGVEVVLLGTGKRLRFPASALMRPFAPAGIGLEVMDLQAA
                     CRTYNILASEGRKVAAALLFD"
     gene            complement(1322..>1566)
                     /locus_tag="PLN02_14190"
     CDS             complement(1322..>1566)
                     /locus_tag="PLN02_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOI53526.1"
                     /translation="IVGNRAIHELVAALVAFGRRLGARVVAEGVETATQLALLRDMGC
                     DAAQGFLFGPPADFAAARDAAGVMATLLATLGKSDS"
BASE COUNT          262 a          483 c          504 g          317 t
ORIGIN      
        1 cgccgagcgc ggtctggatg ccgtgctggc cgggcgcatt ggcgcacagg cgcatcgagg
       61 cgaggatgtc gaggccttcg atgtagtcct tggcatgccg cttgtcgccg gaaaccacca
      121 tccagccggc gcgatagccg caggagcgat agtttttcga cagcccgttg aaggtgatgg
      181 tcagcacgtc gtcggagagc gcggcgatcg aggtgtgctt gacgccgtcg tagagcacct
      241 tgtcgtagac ttcgtcggcg tagatgatca ggtggtgctc gcgggcgatg gcgatgatct
      301 cgtgcagcag gtcgtccggg tacaacgcgc cggtcgggtt gttcgggttg atgatgacga
      361 tggcgcgggt gttcttgttg atcttgctgc ggatgtcgtc gaggtcgggc agccagcctt
      421 tttcctcgtc gcacaggtaa tgcaccggct tgccgccgga caggctgatc gccgccgtcc
      481 acagcgggta gtcgggcgcc ggcaccagca cttcgtcgcc ggcgtcgagc agcgcgttca
      541 tcgccatgac gatcagttcg gaaacgccgt tgccgacgta gatatcctcc agcgtcacac
      601 ccttgatgcc cttctgctgg gtgtagtgca tgatcgcctt gcgcgccgcg aagatgccct
      661 tcgaatccgt gtagccggcc gcgttgggca ggttgcggat gatgtcctgc tggatttcct
      721 cgggcgaatc gaaaccgaag gcggcgaggt tgccgatgtt cagcttgatg atcttgtgac
      781 cttcttcctc catctgcttg gccatctgca gcacggggcc gcggatgtca tagcagacgt
      841 tggcgagttt ggcggatttc ttgacgtgct tcatgggaga atgtccggat tggcggcgtt
      901 ttggccaaag gtataatcct actattacgt ttgctgcacc gcaaagtgaa actccatatc
      961 accccgaatg ccggactcaa cctgtttacc gcctatggcg acgactacgt tgcggtcaac
     1021 cacgaaaaac accagagcag cctgatcgtg ctgccagacc gcctgctcgc cgcgtggacc
     1081 agcgccagtg tcggtagcct gcaggaagcc gacatgcaga ccctgcttgg tctgggcgtc
     1141 gaggtggtcc tgctcggcac cggcaagcga ctgcgctttc cggcgtcggc gttgatgcgc
     1201 ccgttcgcgc cagccggcat cggcctcgaa gtcatggatc tgcaggccgc ctgccgaacc
     1261 tacaacattc tcgccagcga aggccgcaag gtggcggccg ccctgctctt cgactgatcg
     1321 cctagctgtc gctcttaccg agcgtcgcca gcagcgtcgc catcaccccg gcggcatcgc
     1381 gggccgccgc aaaatcagcc ggcggaccga acaggaagcc ctgtgcggca tcgcagccca
     1441 tatcgcgcag cagggcaagc tgcgtcgctg tttcaacgcc ttcggcaacc acgcgtgctc
     1501 cgagccgccg accgaaggcg acgagcgccg cgaccagttc gtggatcgcc cggttgccga
     1561 cgattt
//
LOCUS       DAOSDX010000778         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26443_length_1566_cov_2.545334, whole genome shotgun sequence.
ACCESSION   DAOSDX010000778 DAOSDX010000000
VERSION     DAOSDX010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26443_length_1566_cov_2.545334"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PLN02_14195"
     CDS             complement(<1..1325)
                     /locus_tag="PLN02_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOI53527.1"
                     /translation="MFPAPSRLSWLLLPLLGGCASAPPALPDFALPAQWRLEQPAAPA
                     TPPERLDWQAFGNAELAALIDEALARAPDLAVAAAAERQAEAVARAAGAGRWPGLDLE
                     LRGARNGGSGDNERTRHGASLAASYELDLWGRQQALRDAARAGVDFSRHDRAALRLSL
                     AANVANLWLGVQALRAQIAIAEADRQLVERTLALTEIRLRAGSADPLDVARQRGLLGE
                     RQRSLAALRLDYDNGCLALGALLGRSASVEPQAVDLDQLPLPAVDAGLPSELLRRRPD
                     LARAEAALAAADADVAAARAALLPSINLGASVLAGGSQLADLFASPASSLFATLARPL
                     FDGGRLSARHAAEQARREGLVHAYHAAIVAAFADTQTALNAGAGSAAQLAAQAEVVRQ
                     AEIAWQRAEARYRAGALAMIDLLDSQRTLHAARHQLLAERGEEKMCRKH"
     gene            complement(1330..>1566)
                     /locus_tag="PLN02_14200"
     CDS             complement(1330..>1566)
                     /locus_tag="PLN02_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter permease/ATP-binding
                     protein MacB"
                     /protein_id="HOI53528.1"
                     /translation="QRQFLTEAVLIALSGGLVGAGSGLAIGFALLLWDIPVVFSLTAI
                     VGACACAIATGLVFGWLPARQAARLDPVVALAGE"
BASE COUNT          194 a          536 c          656 g          180 t
ORIGIN      
        1 tcgtgcttgc ggcacatctt ctcttcgcca cgttcggcca gcagctggtg gcgggcggcg
       61 tgcagggtgc gctggctgtc gagcaggtcg atcatcgcca gcgcgccggc acggtagcgg
      121 gcctcggcgc gttgccaggc gatctcggcc tggcgcacga cctcggcctg ggcggcgagc
      181 tgcgcggcgc tgccggcgcc ggcgttgagc gccgtctggg tgtcggcgaa ggcggcgacg
      241 atggcggcgt ggtaggcgtg caccaggcct tcgcggcgcg cctgctcggc tgcgtggcgg
      301 gcgctcaggc ggccgccgtc gaagagcggt cgggccagcg tggcgaacag gctgctggcc
      361 gggctggcga agaggtcggc gagctggctg ccgccggcga ggacgctggc gccgaggttg
      421 atgctgggca gcagggcggc acgggcggcg gcgacgtcgg cgtcggcggc agccagtgcc
      481 gcctcggcgc gggccaggtc ggggcggcgg cgcaacagtt cgctgggcag gccggcatcg
      541 accgccggca gcggcagttg gtcgaggtcc accgcctgcg gctcgacgct ggcgctgcgg
      601 ccgagcaggg cgccgagggc caggcagccg ttgtcgtagt cgaggcgcag cgccgccagg
      661 ctgcgctggc gctcgccgag caggccgcgc tggcgggcga cgtcgagcgg gtcggcactg
      721 ccggcacgca ggcggatttc ggtcagcgcc agggtgcgct cgacgagttg ccggtcggcc
      781 tcggcgatgg cgatctgggc gcgcagcgcc tgcaccccca gccacaggtt ggcgacattg
      841 gcggccaggc tcaggcgcag cgccgcccgg tcgtggcggc tgaaatcgac gccggcgcgg
      901 gcggcgtcgc gcaaggcctg ctggcggccc cagaggtcga gctcgtagct ggcggcaagg
      961 ctggcgccgt ggcgggtgcg ctcgttgtcg ccgctgccgc cgttgcgcgc gccgcgcagc
     1021 tcgaggtcga gaccgggcca gcggccggcg ccggcggcgc gggcgactgc ctcggcctga
     1081 cgctcggcgg ccgcggcgac agccaggtcg ggggcgcgcg ccagggcttc gtcgatcagc
     1141 gccgccagtt cggcgttgcc gaaggcctgc cagtcgaggc gctcgggcgg cgttgccggc
     1201 gctgccggct gctcaagccg ccactgcgcc ggcagcgcga agtcgggaag ggccggcggc
     1261 gcgctcgcgc agccgccgag caggggcagc agcagccagg aaagacggga cggggcaggg
     1321 aacatcgctt cactctccgg caagcgcgac cacagggtcg aggcgcgcgg cctggcgggc
     1381 cggcagccag ccgaacacca ggccggtggc gatggcgcag gcgcaggcgc cgacgatcgc
     1441 ggtcaacgag aaaaccaccg ggatgtccca gagcagcagc gcgaagccga tcgccaggcc
     1501 gctgccggca ccgaccagac cgccgctcag ggcgatcaga acggcctcgg tcaggaactg
     1561 gcgctg
//
LOCUS       DAOSDX010000779         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26461_length_1565_cov_2.133113, whole genome shotgun sequence.
ACCESSION   DAOSDX010000779 DAOSDX010000000
VERSION     DAOSDX010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26461_length_1565_cov_2.133113"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLN02_14205"
     CDS             complement(<1..272)
                     /locus_tag="PLN02_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HOI53529.1"
                     /translation="MRPSDPVTYQCISPAAAAAMIRSDAAATVFDVRDLASYRQGHLE
                     GAMHLQMERVAAWLGRLSRETPLIIYCYHGNASQVYAQMFCDFRFTR"
     gene            complement(422..>1565)
                     /locus_tag="PLN02_14210"
     CDS             complement(422..>1565)
                     /locus_tag="PLN02_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53530.1"
                     /translation="PAASMIGSSDHWRGFYPAPRPILADYIVAGEIEQAMACYEAGHC
                     QASQIVKGAYEAEGFFPVLGDGGCWLYFTAAPLYAPDGRLLGAIETLQNITGRKQAEL
                     RLQEHSDELAQQVALRTEELQQRNTELVDLLRERCELEERVGTANNVAFSAMSSMGEM
                     GVLLHAMQGFNESATAERLAGVVLDALAKYGLEGAVQVRPVGGLACTLPAGEAQVEAI
                     FRHLAEMGRIVNFHRRMVVNFPQISLLLTNLPQGEDERAGRIRDNVTLLAEAAEARLQ
                     ALHNETGRQAGESLIAGALDEIRDLLGQLDKRQQSSLTGSRLAIHAMEESLEKLFVHL
                     GLTDQQESELVATVRDGLDALLEAQSGQGDFHQVLSGITRRLGSFA"
BASE COUNT          269 a          526 c          491 g          279 t
ORIGIN      
        1 cgggtgaagc ggaagtcgca gaacatctgt gcataaacct ggctggcgtt gccgtggtag
       61 cagtagatga tcagcggcgt ttcccgggac aggcggccga gccaggcggc gacccgctcc
      121 atctgcaggt gcatcgcacc ttccagatgg ccctggcgat agctggccag atcgcgcacg
      181 tcgaaaacgg tggcagcggc gtcgctgcgg atcatcgccg ccgcagcggc gggtgagatg
      241 cactggtagg tcacggggtc actggggcgc atgcggtcta cgggctgttg gaatgcctgg
      301 accaagcaag agcgacgcca gcccgggcgg accggatctg gtgccggcgc cgtcttcctc
      361 gctccgtttt ctgtcgcttc gccgacaaac tcgcggcgaa accgacaacg ggaacagcca
      421 ctcaggcgaa gctgccgagg cgacgggtga tgccgctgag aacctgatga aaatcgcctt
      481 gccctgactg cgcttcgagc agggcatcga gtccgtcgcg aacggtggcg accagctcac
      541 tttcctgctg atcggtcaaa ccgagatgga cgaacagctt ctccaggctt tcttccatgg
      601 cgtggatcgc caggcggctg ccggtcagcg acgattgctg gcgcttgtcc agttgaccga
      661 gcaaatcgcg gatctcgtcg agcgctccgg cgatcagact ctccccggcc tgccgccccg
      721 tctcgttgtg caaggcctgc agccgtgcct cggcggcctc ggcgagcagc gtgacattgt
      781 cgcggatacg cccggcccgc tcgtcttcgc cctgcggcaa attggtcagc agaagtgaaa
      841 tttgcgggaa gttgacgacc atccgacggt ggaaattgac gatccgcccc atctccgcca
      901 gatgccggaa aatcgcctcg acttgtgctt ctcctgccgg cagggtgcat gccagcccgc
      961 ccaccggacg gacctggacc gccccttcca gcccgtactt ggcaagcgcg tcgagaacga
     1021 cgccggccag gcgctccgcc gtcgcactct cgttgaaccc ctgcattgcg tgcagcagca
     1081 cgcccatctc gcccatgctg ctcatcgccg aaaaagcgac gttgttcgcc gtgccgacgc
     1141 gctcctccag ttcgcaacgc tcgcgcagca ggtcgaccag ttcggtattg cgctgctgca
     1201 attcctcggt acgcaacgca acctgctgtg ccagttcgtc gctgtgttcc tgcagccgca
     1261 actccgcctg cttgcgcccg gtgatgttct gcaaggtctc gatggcgccg agcagtcggc
     1321 cgtccggggc atacagcggc gcggcggtga aatacagcca gcagccgcca tcgcccagca
     1381 cgggaaagaa gccttcggcc tcgtaagccc ctttgacgat ctgcgacgcc tggcaatgcc
     1441 cggcttcgta gcaggccatc gcctgctcga tctcaccggc gacgatgtaa tcggcaagga
     1501 tcggtcgcgg cgccgggtag aaacctcgcc agtgatcgct gctgccgatc atgctcgccg
     1561 ccggc
//
LOCUS       DAOSDX010000780         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26473_length_1564_cov_2.461895, whole genome shotgun sequence.
ACCESSION   DAOSDX010000780 DAOSDX010000000
VERSION     DAOSDX010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26473_length_1564_cov_2.461895"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLN02_14215"
     CDS             <1..472
                     /locus_tag="PLN02_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53531.1"
                     /translation="EVREQARRPDKRRAGDEEAANPAGNTGELHILLVEDNPINQRLA
                     RALLEKQGHRISLAGHGQEALDLLEALPDIDLILMDLQMPVMDGFEATRQWRQRESAS
                     GRRPLPIIAMTANTMAGDREACLAAGMDGYVAKPVNAAMLKSEIERLQPRAGQA"
     gene            complement(493..>1564)
                     /locus_tag="PLN02_14220"
     CDS             complement(493..>1564)
                     /locus_tag="PLN02_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53532.1"
                     /translation="QSRDGERLYLLFEVCDTGIGIDRETQARLFNPFEQADSSTTRLH
                     GGSGLGLVITRELAELMGGTAGCDSEPGAGSRFWFSASLGVASGTPASRPQLAGESAE
                     ASLVRDCQGARILVCEDNPVNQEVARTLLCDIGLAVDTAANGEAGLALVTRESFDLVL
                     MDVQMPVMDGLEATRRIRALPQGRELPILAMTANAFAEDRKTCLACGMNDFVAKPVDP
                     DALYAALLRWLPRRPPRPAPPSDPEVALASLPGFDAAALLRIVRGQSGRAVKLLRMFA
                     DGHHDDAARLRSLLAAGETQAAQQLAHALKGAAGSLSLQLVHRLAGEISDGLRRDEAV
                     TIDALAAALAGVCAGIAELPEG"
BASE COUNT          262 a          561 c          542 g          199 t
ORIGIN      
        1 cgaagtccgc gagcaggcca ggcggccgga caaacgccgc gccggtgacg aggaagcggc
       61 caatccggcc ggcaacaccg gggaactgca catcctgctg gtcgaggaca acccgatcaa
      121 ccagcgactg gccagggctc tgctggaaaa gcagggacac cggatcagcc ttgccggcca
      181 tggccaggaa gccctcgacc tgctggaagc cctgcccgac atcgacctga tcctgatgga
      241 cctgcagatg ccggtgatgg acggcttcga agccacccgc cagtggcgcc agcgggaaag
      301 cgccagcggc cgccggccgc tgccgatcat cgcgatgacc gccaacacca tggccggcga
      361 ccgcgaagcc tgcctcgccg ccggcatgga cggctacgtg gcgaagccgg tcaacgcggc
      421 gatgctgaaa agcgagatcg aacgcctgca gccgcgggcg ggccaggcct gagcggcgcc
      481 agcgccgccg cttcagcctt ccggcagctc ggcgatgcca gcgcagacgc cggccagcgc
      541 ggcggccagc gcgtctatcg tcaccgcttc gtcgcgccgc aggccatcgc tgatctcgcc
      601 ggccagacga tggacaagct gcagcgacaa actgccggcg gcgcccttca gggcatgcgc
      661 cagttgctgt gcggcctgtg tctccccggc ggcgagcaag gagcgcaggc gcgccgcgtc
      721 gtcatggtgg ccatcggcga acatgcgcag cagcttcacc gcgcgcccgc tctgaccgcg
      781 cacgatgcgc agcagcgccg cggcgtcgaa gcccggcagg ctggccagcg ccacctcggg
      841 atcggacggc ggcgccggac gcggcggacg gcgcggcagc cagcgcagca gcgcggcata
      901 gagcgcgtcg ggatcgaccg gcttggcgac gaaatcgttc atgccgcaag ccaggcaggt
      961 cttgcgatcc tcggcgaagg cgttggcggt catcgccaga atcggcagtt cccgcccctg
     1021 cggcagcgcg cggatgcggc gcgtcgcttc gagaccatcc atcaccggca tctgcacgtc
     1081 catcagcacc agatcgaagg attctcgcgt caccagcgcc aggccggcct cgccgttggc
     1141 cgcggtgtcc acggccaggc cgatgtcgca gagcagcgtg cgcgccacct cctgattgac
     1201 cgggttgtcc tcgcagacga ggatgcgggc gccctggcaa tcgcggacca ggctcgcttc
     1261 cgccgattcc ccggcgagtt gcgggcgtga cgccggcgta ccgctggcca cgcccaggct
     1321 ggccgagaac cagaaacggc tgcccgcccc cggctcgctg tcgcaaccgg cggtaccgcc
     1381 catcagttcg gccagttcgc gggtgatcac caagccgagg ccgctgccgc cgtgcaggcg
     1441 ggtggtcgag ctatcggcct gctcgaaggg attgaacaag cgagcctggg tttcccggtc
     1501 gatgccgatg ccggtatcgc agacttcgaa cagcaggtag aggcgctcgc cgtcgcgcga
     1561 ctgc
//
LOCUS       DAOSDX010000781         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26494_length_1563_cov_1.963528, whole genome shotgun sequence.
ACCESSION   DAOSDX010000781 DAOSDX010000000
VERSION     DAOSDX010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26494_length_1563_cov_1.963528"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..906)
                     /locus_tag="PLN02_14225"
     CDS             complement(151..906)
                     /locus_tag="PLN02_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI53533.1"
                     /translation="MTDNLLEVVGLTRRFAAVEALKAVDFVVAAGEVHALIGPNGAGK
                     TTFIHHLSGALLPDAGQIRFAGRDVTRLSMHERVRAGMARSYQITNVFLGLAALDNVA
                     LAVQGRPDAGSSFRFWRPVRREARLFDEAREFLAQVGLAAQAATLAGNLSHGEQRALE
                     LAMALATKPQLLLLDEPMAGTGPEESARMVELIEHLARHVTILLVEHDMDAVFRLADR
                     ISVLEGGELICTGTPDEVRNDAGVRRAYLGDHL"
     gene            complement(899..>1563)
                     /locus_tag="PLN02_14230"
     CDS             complement(899..>1563)
                     /locus_tag="PLN02_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOI53534.1"
                     /translation="AMVVAGLFALLVGAISLRTRGVYFIMITLAFAQMVYYLFVSASA
                     WGGDDGLPIYERLQLGSFSLAGDAALYYTALGALALAMFLFGRLADARFGRTVQAIRE
                     NETRMEALGYPVFRYRLVCFALGGACAGLAGALLANQSGLASPSLLQWTQSGTLLVMV
                     IIGGVGYLYGGVVGAIVLLGLEELLLSWFAHWHIVLGLLLLGIVLFAPKGVAALFGRS
                     RD"
BASE COUNT          274 a          548 c          545 g          196 t
ORIGIN      
        1 cgcacagggc gcgcagcgtc gtcgtcttgc ccatgccgtt gcgaccgagc aaggtcgccg
       61 cctcgccctc ggccatggtc aggtcgatgc cgaacagcac ctggctcggg ccgtaggcga
      121 cttccaggcc gacgatttcg agcagcgcgc tcacaggtgg tctcccaggt aggcgcggcg
      181 cacgccggca tcgttgcgca cttcgtccgg cgtgccggtg cagatcagct cgccgccctc
      241 cagcaccgag atgcggtcgg cgaggcggaa cacggcgtcc atgtcgtgct cgaccaggag
      301 gatcgtgacg tgacgggcga ggtgttcgat cagctcgacc atgcgcgccg attcctcggg
      361 accggtgccg gccatcggct cgtcgaggag cagcagttgc ggtttggtcg ccagcgccat
      421 cgccagctcc agcgcccgct gctcgccgtg cgagagattg ccggcgagcg tcgccgcctg
      481 tgcggcgagg ccgacctggg cgaggaactc ccgggcttcg tcgaacagcc gggcctcgcg
      541 ccggaccggc cgccagaagc ggaagctgct gccggcgtcg ggccgcccct ggaccgccag
      601 cgcgacgttg tcaagggcgg cgagaccgag gaaaacgttg gtgatctggt aggaacgcgc
      661 catgccggcg cgcacccgct cgtgcatcga cagccgggtg acgtcgcggc cggcgaagcg
      721 gatctggccg gcgtccggca acagcgcccc cgacaggtga tggatgaagg tcgtcttgcc
      781 ggcgccgttg gggccgatca gcgcatgcac ctcgccggcg gcgaccacga aatcgaccgc
      841 cttcagcgcc tcgaccgcgg caaagcgacg cgtcagcccg acaacttcga gcagattgtc
      901 agtcacgcga cctcccgaac agcgcggcga cgcccttcgg cgcgaacagc acgatgccga
      961 gcaggagcag gccgagaacg atgtgccagt gcgcgaacca gctcagcagc agctcctcca
     1021 gaccgagcag gacaatggcg ccgacgacgc cgccgtagag gtagccgacg ccgccaatga
     1081 tcaccatcac cagcagcgtg cccgactgcg tccattgcag caggctgggg ctggccagcc
     1141 cgctctggtt ggcgagcagc gccccggcca ggccggcgca ggcgccgccc agcgcaaagc
     1201 agaccaggcg gtagcggaag accgggtagc cgagcgcctc catgcgcgtc tcgttctcgc
     1261 ggatcgcctg cacggtgcgc ccgaaacggg catcggccag gcgcccgaaa aggaacatgg
     1321 cgagcgccag cgcgccgagc gcggtgtagt agagcgccgc gtcgccggcc agactgaagc
     1381 tgccgagctg caggcgctca tagatcggca ggccatcgtc gccgccccag gcgctggcgg
     1441 agacgaacag gtaatagacc atctgggcga aggccagcgt gatcatgatg aagtagacgc
     1501 cacgcgtgcg caggctgatc gccccgacca ggagcgcgaa cagcccggcc accaccatcg
     1561 cca
//
LOCUS       DAOSDX010000782         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26507_length_1562_cov_2.873922, whole genome shotgun sequence.
ACCESSION   DAOSDX010000782 DAOSDX010000000
VERSION     DAOSDX010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26507_length_1562_cov_2.873922"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLN02_14235"
     CDS             complement(<1..654)
                     /locus_tag="PLN02_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOI53535.1"
                     /translation="MIKIGLVGYGKAGQAVASVLREDPRFELCWVARGSSAESIDDLP
                     VVKLEADSFGAWLDAHPVDGIIDFSNPQAVLSYGEAVRQRGIMVVTAISGYPPETLDY
                     IRDLGRDIRIMSSPNITLGINFLLLAAQILRRIAPFADVEIVEQHFRDKPEVSGTARR
                     IAEVLDVDDERITSLRLGGIVGHHEVIFGFPYQTVRLIHDSIRREAFGRRKWNYRPEE
                     "
     gene            1026..1457
                     /locus_tag="PLN02_14240"
     CDS             1026..1457
                     /locus_tag="PLN02_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOI53536.1"
                     /translation="MRRHFRLVEERCGMSGSQMWVLQEVQRSPGIGIGELAERMGVHQ
                     STCSQLVDKLAAQDCLDKRKKTGDQRRVGLQLTERGHEALAALPGPAEGVVPEALAAV
                     PEVVLKTLQINLAELLQHIPGKDEKYANMPLADMVRNNEGD"
BASE COUNT          306 a          460 c          502 g          294 t
ORIGIN      
        1 ttcctcgggc cggtagttcc atttgcggcg accgaaggcc tcgcggcgga tcgagtcgtg
       61 gatcaggcgc acggtctggt aggggaaacc gaagatcact tcgtggtggc cgacgatgcc
      121 gcccaggcgc agcgaggtga tgcgctcgtc gtcgacgtcg agcacctcgg caatgcgtct
      181 cgccgtgccg gagacttccg gcttgtcgcg gaaatgctgc tcgacgatct cgacatcggc
      241 gaagggcgcg atgcggcgca gtatctgcgc cgccagcagc aggaagttga tgcccagcgt
      301 gatgttgggc gacgacatga tgcggatgtc gcgaccgagg tcgcggatgt aatccagggt
      361 ttccggcgga tagccggaaa tcgccgtcac caccatgatg ccgcgctggc gcacggcttc
      421 gccatagctg aggacggctt gcgggttcga aaagtcgatg attccgtcga ccgggtgggc
      481 atccagccag gcgccgaagc tgtccgcttc aagcttcacg acgggcaggt cgtcaatgct
      541 ctcggcggac gaaccgcgtg ccacccagca caactcgaaa cgcggatctt cccgcaggac
      601 gctggcaacg gcctgtccag ccttcccgta accaacgagt ccgatcttga tcatggtttc
      661 tcctccaaat gcagtttgcg atctccaggc cgtcccggtt gtcgggcgac gaaaagcggt
      721 acgtcccggt tggggggcgt tcatgtacgt tctttgacgc atctcgggcg ccggcaaaag
      781 gtgcggcgcg cgctgctggg cgacgggcgg aaaaaaaagg ggggttgcac cccccaatgg
      841 aaaacctcgt cgaaaaggaa tggggttatg acctaagtta caactccatt ggcgctcaat
      901 ataattgagg cttaaaattc gcgcaatact attatttgaa agtaccagtt ttgaacgctt
      961 tgagtagctc cagggagcag cgggtcattg atgtcctgca acagttccgt ctgatttacg
     1021 ggtccatgcg ccggcatttc cggctggtcg aagaacgctg cggcatgtcc ggttcgcaga
     1081 tgtgggtgtt gcaggaggtg cagcgttcgc cgggtatcgg catcggcgaa ctcgccgagc
     1141 gcatgggcgt tcaccagtcc acctgcagcc agttggtaga caagctggcg gctcaggatt
     1201 gcctcgacaa gcgcaagaag acgggcgatc agcgccgggt cggcctgcag ttgaccgagc
     1261 gcggtcacga ggccctggcc gccttgcccg gtccggccga aggcgtcgtt ccggaagccc
     1321 tggcggcggt gccggaagtc gtcctgaaaa cattgcagat caatcttgcg gagctgctgc
     1381 agcatatccc tggcaaggac gagaaatacg ccaacatgcc gctggccgac atggtccgca
     1441 ataacgaagg agattgattt gccgaccacc aagctagcga ttgtcctcgg cgccgtactg
     1501 ttgctcggcg cttgcgccgc tcaaccgcca gtcagcgaaa ccccgccgcg ggaaaccccc
     1561 cc
//
LOCUS       DAOSDX010000783         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26580_length_1558_cov_3.465070, whole genome shotgun sequence.
ACCESSION   DAOSDX010000783 DAOSDX010000000
VERSION     DAOSDX010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26580_length_1558_cov_3.465070"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="hisD"
                     /locus_tag="PLN02_14245"
     CDS             complement(<1..905)
                     /gene="hisD"
                     /locus_tag="PLN02_14245"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOI53537.1"
                     /translation="MTIAIQRLSTVAADFPAQLKTLLAFEAAADDNIERTVAAILADV
                     KTRGDAAVVDYSNRFDRLTASSMADLELSRDELEKALAGLPAERRAALEAAAQRVRAY
                     HEKQRLDGWSYTEADGTLLGQMITPLDRVGLYVPGGKAAYPSSVLMNAIPAKVAGVKE
                     LIMVVPTPGGEHNQLVLAAACLAGVDRVFTIGGAQAVGALAYGTESVPQVDKIVGPGN
                     AYVATAKRRVFGIVGIDMVAGPSEILVVSDGTGNPDWVAMDLFSQAEHDELAQSILIC
                     TDAAFIERVQASIEKLLPTMPRQEV"
     gene            complement(906..1550)
                     /gene="hisG"
                     /locus_tag="PLN02_14250"
     CDS             complement(906..1550)
                     /gene="hisG"
                     /locus_tag="PLN02_14250"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOI53538.1"
                     /translation="MTTITIALSKGRIFDETLPLLAAAGIVPAENPETSRKLIIETNQ
                     PQVRVVIVRATDVPTYVQYGAADIGVAGKDVLIEHGGDGLYAPLDLNIAKCRMMVAVP
                     EGFDYANAVRQGARLKVASKYIKTAREHFAAKGVHVDLIKLYGSMELAPLAGLADAIV
                     DLVSTGGTLKANKLVAVEHILDISSRLVVNQAALKVKRETLQPIIDAFAQAVEK"
BASE COUNT          233 a          513 c          537 g          275 t
ORIGIN      
        1 atcacttcct ggcgcggcat cgtcggcagc agcttctcga tgctcgcctg gacgcgctcg
       61 atgaaggcgg cgtcggtgca gatcaggatc gattgcgcca gttcgtcgtg ctcggcctgc
      121 gagaacaggt ccatcgccac ccagtcgggg ttgccggtac cgtccgagac gaccaggatt
      181 tccgacgggc cggcgaccat gtcgatgccg acgatgccaa acacccggcg cttggccgtc
      241 gccacgtagg cattgcccgg gccgacgatc ttgtccacct gcggcacgct ctcggtgccg
      301 taggcgagcg cgccgaccgc ctgcgcgccg ccgatggtga acacgcggtc gacgccggcc
      361 aggcaggccg ccgccagcac cagctggttg tgctcgccgc ccggcgtcgg cacgaccatg
      421 atcagttcct tgaccccggc caccttggcc gggatcgcgt tcatcagcac cgacgacgga
      481 taggccgcct tgccgcccgg cacgtagagg ccgacgcgat cgagcggggt gatcatctgg
      541 ccgagcaacg tgccgtcggc ttcggtgtag gaccagccgt cgagcctctg cttctcgtgg
      601 taggcacgca cgcgctgggc ggccgcttcg agcgcggcac ggcgctcggc aggcaggccg
      661 gcgagcgcct tttccagttc gtcgcgcgac agttcgaggt cagccatcga gcttgccgtc
      721 aggcgatcga agcggttgct gtagtcgacc acggcggcgt cgccgcgcgt cttgacgtcg
      781 gccaggatgg cggcgacggt gcgttcgatg ttgtcgtcgg cggccgcttc gaaggcgagc
      841 agcgtcttca attgcgccgg gaaatcggcg gctaccgtgg acaaacgctg gatggctatc
      901 gtcatttact tctccaccgc ctgcgcgaag gcgtcgatga tcggctgcag ggtttcgcgc
      961 ttgaccttga gtgccgcctg gttgaccacc aggcgcgagg aaatgtcgag gatgtgctcg
     1021 acggcgacca gcttgttggc cttcagcgtg ccgccggtcg acaccaggtc gacgatggcg
     1081 tcggccagac cggccagcgg cgccagctcc atcgagccgt aaagcttgat caaatcaacg
     1141 tgcacgccct tggcggcgaa gtgctcgcgc gccgtcttga tgtacttcga ggccaccttc
     1201 aagcgggcgc cctggcgcac cgcattggcg tagtcgaagc cttccggcac ggcgaccatc
     1261 atccggcact tggcgatgtt caggtcgagc ggcgcgtaga ggccatcgcc gccatgttcg
     1321 atcagcacgt ccttgccggc gacgccgatg tcggccgcgc cgtactggac gtaggtcggc
     1381 acgtcggtgg cgcggacgat gacgacgcgc acctgcggct ggttggtctc gatgatcagc
     1441 ttgcgcgagg tctccggatt ctcggccggg acgatccccg ccgccgccag cagcggcagg
     1501 gtttcgtcga agatgcggcc cttcgagagg gcgatggtga tggtcgtcac gggggttc
//
LOCUS       DAOSDX010000784         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26599_length_1557_cov_3.547270, whole genome shotgun sequence.
ACCESSION   DAOSDX010000784 DAOSDX010000000
VERSION     DAOSDX010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26599_length_1557_cov_3.547270"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLN02_14255"
     CDS             complement(<1..328)
                     /locus_tag="PLN02_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOI53539.1"
                     /translation="MLYPTRFDVIVVGGGHAGTEAALAAARAGAATLLLTHNLETLGA
                     MSCNPSIGGIGKGHLVREVDALGGAMAAATDEAGIQFRILNASKGPAVRATRAQVAGC
                     AIAIVAR"
     gene            471..>1557
                     /locus_tag="PLN02_14260"
     CDS             471..>1557
                     /locus_tag="PLN02_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOI53540.1"
                     /translation="MNHKTAGNGSPLDDEEMLLVLQHSLTKKRVLLIDRHPPARDALR
                     LMLGALGVTAVHGAGNSAEVIRQVKANRFDIILSDFVLDDGRDGQQLLEELRHAHLIP
                     LATVYMIITSERGYTNVVALAELAPDDYLIKPFTAEQLQGRLVRAIYKKHVLRKIYEH
                     IEASELLEAVASCDRVIQQQPLYMYDALRFKGELLHQLGRTQEAEEVYRRVLEGRVVP
                     WAKMGLATALRDRNALDEAKQLAEQVLLEAPEFLSAYDFLASVHEAQGRLQEAQLVLQ
                     RAAEASPHNTVRQRLVGDVAARNKDFLVAEKAYSKVMERSRGSLLRSVDDFANLSRVL
                     VERGDVEASRRIVGEMKRELRGDKQATL"
BASE COUNT          306 a          538 c          471 g          242 t
ORIGIN      
        1 cgcgggccac gatggcgatc gcgcacccgg cgacctgggc gcgcgtcgcg cgcaccgccg
       61 gacctttcga cgcattgagg atgcggaact ggatgccggc ctcatcagtc gccgccgcca
      121 tcgccccgcc cagcgcatcg acctcgcgca ccagatggcc cttgccgatg ccgccgatcg
      181 acgggttgca gctcatcgcc ccgagcgttt cgaggttgtg cgtcagcaac aacgtggccg
      241 caccggcccg cgccgcggca agcgcggctt cggtaccggc gtggccgccg ccgacgacga
      301 tgacatcgaa acgggtggga taaagcatgg gaaaatgtct tgttgcggcg cagtatggga
      361 ccggcgattt tacgtcaggc aactggaaaa tcacagactt aagcaagcca ggcaaacaca
      421 acacacagtt cggcttgccg tcgccgggct tctacagtaa ttttccggaa atgaatcaca
      481 aaaccgccgg caacggcagc ccgctcgacg acgaggaaat gctgctcgtc ctccagcact
      541 cgctgaccaa gaaacgtgtc ctgctcatcg accgccaccc gccggcgcgc gacgcgctgc
      601 gcctgatgct cggcgcgctc ggtgtgaccg cggtgcatgg cgccggcaac tcggccgaag
      661 tgatccgcca ggtcaaagcc aaccgtttcg acatcatcct ctccgacttc gtcctcgacg
      721 atggccgcga cggccagcaa ctgctcgagg aactgcgcca cgcccacctg attccgctgg
      781 cgacggtgta catgatcatc accagcgaac gcggctatac caacgttgtc gccctcgccg
      841 aactggcccc cgacgattac ctgatcaagc ctttcaccgc cgagcaactg cagggacgcc
      901 tggttcgcgc catctacaag aagcatgtgc tgcgcaagat ctacgagcac atcgaagcca
      961 gcgaactgct ggaggcggtc gcctcctgcg accgcgtgat ccagcagcaa ccgctgtaca
     1021 tgtacgacgc gctacgcttc aagggcgaac tgctgcacca gctagggcgc acccaggaag
     1081 ccgaggaagt ctatcgacgc gtcctcgaag gccgtgtcgt tccctgggca aagatgggcc
     1141 tggccaccgc gctgcgcgac cgcaacgccc tcgacgaagc gaagcagctc gccgaacagg
     1201 tcctgctcga agccccggaa tttctctccg cctacgactt cctggcctcg gtccatgaag
     1261 cccagggccg cctccaggag gcgcaactgg ttctgcaacg ggccgccgaa gcctcgccgc
     1321 acaacaccgt gcggcagcgc ctggtcggcg acgtcgccgc ccgcaacaag gatttcctgg
     1381 tcgccgagaa agcctacagc aaggtcatgg agcgcagccg ggggtcgttg ttacgcagcg
     1441 tcgacgattt cgccaacctg tcgcgcgtcc ttgttgagcg cggcgatgtc gaagcgtcgc
     1501 ggcgcatcgt cggcgagatg aagcgtgaac tgcgcggcga caagcaggcg acactcg
//
LOCUS       DAOSDX010000785         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26715_length_1552_cov_2.937208, whole genome shotgun sequence.
ACCESSION   DAOSDX010000785 DAOSDX010000000
VERSION     DAOSDX010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26715_length_1552_cov_2.937208"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /gene="surE"
                     /locus_tag="PLN02_14265"
     CDS             <1..731
                     /gene="surE"
                     /locus_tag="PLN02_14265"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOI53541.1"
                     /translation="LSNDDGYFAPGLAALAEALVGLGEIVVVAPEQNRSGASNSLTLD
                     RPLMLKQAANGFYFVNGTPTDCVHLAVTGMLDYLPDIIVSGINSGANMGDDTIYSGTV
                     AAAMEGYLLGIPAIAMSMTSFDGLNFATAGRVARELVEHYLDNPIEGPVLLNVNVPDI
                     PYEQLTGREVTRLGRRHKAEPVVRMTSPRKETVYWIGAAGAAADAGPGTDFHAVEQKR
                     VSITPLQIDLTQVSQLPTIRAWLQ"
     gene            728..1390
                     /locus_tag="PLN02_14270"
     CDS             728..1390
                     /locus_tag="PLN02_14270"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOI53542.1"
                     /translation="MTVRGLQGIGMTSQRTRARMIDRLREKGIRSEKVLAAMAAVPRH
                     AFVEEALASRAYEDTALPLGMGQTISQPYIVARMIELLLNGRPGLGKTLEVGAGCGYQ
                     AAVLAQLSKDVFALERIGPLLEKAKANMRTLQQFNVRLKLADGNFGLPEAAPFDSIIV
                     AAAGPLIPPELLRQLAPGGRMVLPVGGQEQYLCLVEHTPNGFTETRLDPVRFVPLLSG
                     IQ"
BASE COUNT          281 a          447 c          489 g          335 t
ORIGIN      
        1 tgctcagtaa tgacgacggc tatttcgcgc cggggcttgc cgcgctggcg gaggccctgg
       61 ttggcttggg agaaatcgtc gtcgtcgcgc ccgagcagaa tcggagtggc gccagtaatt
      121 cattgacgct ggatcggccg ctgatgctga aacaggcggc caacggattt tatttcgtca
      181 atggtacacc gaccgactgc gtgcatctgg cggtaaccgg aatgctggat tacctgccgg
      241 atatcatcgt ttccggaatc aactcgggcg ccaatatggg cgacgacacg atttattcag
      301 gaaccgttgc cgccgccatg gaaggttatc tactcggcat tccggcgatt gcgatgtcga
      361 tgaccagttt tgacggattg aattttgcaa ctgccggtcg agttgcccgc gaactggtcg
      421 agcattatct cgataatccg atcgaggggc ccgtactctt gaatgtcaat gtgccggata
      481 ttccctacga acaattgacg ggcagggagg tgacgcgtct cggacgccgg cacaaggcgg
      541 agcccgtcgt acgcatgacc tcgccgcgca aggaaacggt ttactggatc ggtgccgccg
      601 gtgccgccgc cgatgccggg cccggtactg attttcacgc ggttgagcag aagcgggttt
      661 cgatcacgcc gctgcagatc gacctgacac aggtcagtca gttgccaacg atccgggctt
      721 ggttgcaatg acggttcgtg gtttgcaggg gatcggcatg acttcgcagc gtacgcgtgc
      781 gcggatgatc gaccgcttgc gcgaaaaggg tatccgcagc gagaaggtcc tggcggcaat
      841 ggcggcggtg ccgcgccacg ctttcgtcga ggaggcgctg gcttcccgcg cctacgagga
      901 cacggccctg ccgctgggca tggggcagac gatttcccag ccctacatcg ttgcgcggat
      961 gatcgagttg ttgctcaatg gtcgtccagg cttgggcaag acgctcgaag ttggcgctgg
     1021 ttgcggctac caggcggcag tgctggcgca gttgagcaag gatgtctttg cgcttgaacg
     1081 gatcggtcct ttgctggaga aggccaaggc caacatgcgc acgctgcagc agttcaatgt
     1141 gcgcctgaaa ctggctgacg gcaatttcgg tttgccggaa gcggcgcctt tcgacagcat
     1201 catcgtcgcc gccgccggtc cgcttattcc gccggaattg ctccgtcagt tggcgcccgg
     1261 tggtcgaatg gtcttgcctg tcggggggca ggagcagtat ctttgccttg ttgaacacac
     1321 cccaaacggg tttacggaga cgcgactgga ccccgtccgg ttcgtgcctt tgttatccgg
     1381 aatacaatga tcaaacccct tctcctggtt tcactcctcc tttctctcgc cgcctgttca
     1441 caggcgccag cgccgcttgc caatccttcg ccgacagctc agagccgagt cgccggcccc
     1501 ggggaatact tggtcaaacg tggcgatacc ctgtatcgca tcgcgcgcga ga
//
LOCUS       DAOSDX010000786         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26738_length_1551_cov_2.529412, whole genome shotgun sequence.
ACCESSION   DAOSDX010000786 DAOSDX010000000
VERSION     DAOSDX010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26738_length_1551_cov_2.529412"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1161
                     /locus_tag="PLN02_14275"
     CDS             301..1161
                     /locus_tag="PLN02_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53543.1"
                     /translation="MFAALRRLPAGLRPRRCDTDILVSLDGHGAAQRASLLFASATPL
                     KLSWSELPLDPAGEILTMYGAALAAPAAGSGEAGALTLAGLGEYLDLPDVDVRQPPPG
                     GPRFACLTPVARLNREVWALFAQLLNDDPAASLTLNLGELDDAAGQRVAADFAAYQIA
                     PQRLHFVRASDAKALCQAWNDVDVGLAPLHGDGDQALPTCLWMNRPYVAIDSPLPWSR
                     RPAALLRLAGLAHCVAADAADYPARARQLGTSREKVAGRSALRASLASSAESFVADFS
                     ERILQAFRAS"
     gene            1158..>1551
                     /locus_tag="PLN02_14280"
     CDS             1158..>1551
                     /locus_tag="PLN02_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOI53544.1"
                     /translation="MNPTPSFSVIVCSIDAGKFAQISAAYRRLLAGHRCEIIGIHDAA
                     SLAAGYNRGIQQASGDILIFSHDDILILDPDFAAKVVHRLTHFDLLGFAGASKLVSDL
                     WAGADLPFLHGVIAHAPAQSRQITLDVFG"
BASE COUNT          231 a          594 c          481 g          245 t
ORIGIN      
        1 ccgggtcgcc ccctaccaga gcctgctgat ccggatgctc agccgcagcg acgccgcccc
       61 cggcgaaatc ggccaggaga tcgccagttt ccgccagcat ttcccggcgc cgccgatgcg
      121 tcggccccgc cgacgccacg ctggagaggc cgtttccata gccttcctga tcagcgattt
      181 cggccagccg acggcggcca gacagctcga agccctgttc cgcctgctgc cggcgagcga
      241 atttcgcacc atcctggtca acaacgaccc ctacagtgcc ggcaacgagc tggccgagcg
      301 ttgttcgctg ctctgcgacg cctacctgca ggccttcgac cgcgacgatg cgacaccgac
      361 atcctggtca gcctggacgg gcacggtgcg gcgcagcggg cgagcctgct gttcgcctcg
      421 gcgacgccac tgaagctgtc gtggagcgaa ctgccgctcg atccggcggg cgagatcctg
      481 accatgtacg gcgccgccct cgccgcgccg gcagcgggca gcggggaggc cggtgcgttg
      541 acgctggccg gcctgggcga atacctcgac ctgcccgacg tcgatgtccg gcagccgccg
      601 ccgggcggcc cccgcttcgc ctgcctgacg ccggtcgcca ggctgaaccg ggaagtctgg
      661 gcactcttcg cccaactgct gaacgacgac cccgccgcct cgctgaccct caacctgggc
      721 gaactggacg acgcagccgg gcagcgggtg gccgccgact tcgccgctta ccagatagcg
      781 ccgcaacggc tgcacttcgt ccgcgccagc gacgcgaaag cgctgtgcca ggcgtggaac
      841 gacgtcgatg tcggcctggc gccgctgcat ggcgacggcg atcaggcgct gccgacctgt
      901 ctgtggatga accgccctta cgtggcgatc gactcgccgt tgccctggtc gagacgcccg
      961 gccgcgttgc tgcgcctggc cggtctggcg cattgcgttg ccgccgatgc cgccgactat
     1021 cccgcccgcg cccggcaact cggcaccagc cgggaaaagg tggccggccg atccgccctg
     1081 cgcgcttctc tggcaagcag tgccgagtcc ttcgtcgccg acttctccga acgcatcctg
     1141 caggctttcc gcgcatcatg aatccaaccc cgagcttctc cgtcatcgtc tgcagcatcg
     1201 acgcaggcaa attcgcccag atcagcgccg cataccggcg cctgctcgcc ggccaccgct
     1261 gcgagatcat cggcatccac gacgccgctt cgctggccgc aggctacaac cgcggcatcc
     1321 agcaggcgag cggcgacatc ctgatcttct cccacgacga catcctgatc ctcgacccgg
     1381 atttcgccgc caaggtcgtg caccgcctga cgcatttcga cctcctcggc ttcgccggtg
     1441 ccagcaagct ggtcagcgac ctgtgggcgg gcgccgacct gcccttcctg cacggcgtca
     1501 tcgcgcacgc cccggcgcaa tcgcggcaaa tcaccctcga tgtctttggt g
//
LOCUS       DAOSDX010000787         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26782_length_1549_cov_2.942436, whole genome shotgun sequence.
ACCESSION   DAOSDX010000787 DAOSDX010000000
VERSION     DAOSDX010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26782_length_1549_cov_2.942436"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLN02_14285"
     CDS             complement(<1..428)
                     /locus_tag="PLN02_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOI53545.1"
                     /translation="MSALRLSLAQINPTIADIAGNIALMTAAAQNAAVDGADMVIFPE
                     LSLTGYWPGDLLDETDFLARVDAGLAQLAAASRATPGLCWVVGAPIRRTGPGKRLHNS
                     LLVLRDGEVVLSYAKQLLPTYNVFDESRHFAPGPDVTRVLR"
     gene            complement(425..>1549)
                     /gene="pncB"
                     /locus_tag="PLN02_14290"
     CDS             complement(425..>1549)
                     /gene="pncB"
                     /locus_tag="PLN02_14290"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOI53546.1"
                     /translation="PGAQAEYAFVCRNTPLFPLAELKPAVDAELDRLCSLTFADDELA
                     YLRSLRFIKSDFVDFLTVFRFQRRFIETRVNGDKLEIVARGPLVHVMAFEIFVLYIVN
                     ELYFRRLPVDDKLLTVARQRLREKIDFIRGLGDELCSADEPFVFFDFGLRRRYSGAWH
                     AEVVRTLAAELPVNFRGTSNVLFAKEMGLTPIGTMAHEYLQAYQSFGFRLRDFQKAAL
                     EGWVQEFRGDLGVALTDVVGMDAFLGDFDLYFAKLFDGLRHDSGDPVEWAEKALAHYR
                     KLHIDPRSKRLVFSDGLDLPRAVELFRRYRGRVMTSYGIGTNLSNDTPFAALNVVMKI
                     VACNGQPVAKLSDSPGKTLCDDQTFLAYLRQVFGQKGGGS"
BASE COUNT          294 a          489 c          533 g          233 t
ORIGIN      
        1 cgcagcacgc gggtgacatc cggccccggc gcgaagtggc gcgattcgtc gaagacgttg
       61 taagtcggca gcaactgctt ggcgtagctc aacaccacct cgccgtcgcg cagcacgagc
      121 aggctgttgt gcaggcgctt gcccggcccc gtgcgccgga tcggcgcgcc aaccacccag
      181 cacaggccgg gcgtggcgcg gctggcagcg gcgagttgcg ccaagccggc atcgacgcga
      241 gcaaggaaat ccgtttcatc gagcaagtcg cccggccagt agccggtcag cgacagttcc
      301 gggaaaatca ccatatcggc cccgtcgacc gcagcatttt gcgcggcggc ggtcatcagc
      361 gcgatgttgc cggcaatgtc agcaatggtc gggttgatct gggccagcga cagacgcagc
      421 gcgctcatga tccaccgccc ttctgcccga acacctggcg caggtaggcc aggaaggtct
      481 ggtcgtcgca cagggtcttg cccggcgagt ccgagagctt ggccaccggc tggccgttgc
      541 aggcgacgat cttcatgacc acgttgagcg cggcaaaggg cgtgtcgttg gacaggttgg
      601 tgccgatgcc gtaggaggtc atgacgcggc cgcgatagcg ccggaacaac tcgacggcgc
      661 gcggcagatc gaggccgtcg gagaagacca gccgcttgct gcgcgggtcg atatgcagct
      721 tgcggtagtg cgccagcgcc ttttccgccc actcgaccgg gtcgccggaa tcgtgccgca
      781 ggccgtcgaa aagcttggcg aagtagaggt cgaagtcgcc gaggaaggcg tccatgccga
      841 ccacgtcggt cagcgccacg ccgaggtcgc cgcggaattc ctgcacccag ccttccagcg
      901 ccgccttctg gaaatcgcgc aggcggaagc cgaaggactg gtaggcctgc aggtattcgt
      961 gcgccatggt gccgatcggg gtcaggccca tttccttggc gaagagcacg ttcgaggtgc
     1021 cgcggaaatt gaccggcagc tcggccgcca gcgtgcgcac cacttccgcg tgccaggcac
     1081 cggaataacg gcggcgcagg ccgaaatcga agaagacgaa aggctcgtcg gccgagcaca
     1141 gctcgtcgcc gaggccgcgg atgaagtcga tcttctcgcg caggcgctgg cgcgccaccg
     1201 tcagcagctt gtcatccacc ggcaggcggc ggaagtacag ttcattgacg atgtagagca
     1261 cgaaaatctc gaaggccatc acatgcacca gcggcccgcg cgcgacgatc tccagcttgt
     1321 cgccattgac gcgggtttcg atgaagcggc gctggaagcg gaagacggtg aggaaatcga
     1381 cgaaatcgct cttgatgaaa cgcaggctgc gcagataggc cagctcgtcg tcggcgaagg
     1441 tcagcgaaca gagccggtcc agctcggcat ccaccgccgg cttcaactcg gcgagcggga
     1501 acagcggcgt gttgcggcag acgaaggcgt actcggcctg ggcgccggg
//
LOCUS       DAOSDX010000788         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26832_length_1547_cov_3.615282, whole genome shotgun sequence.
ACCESSION   DAOSDX010000788 DAOSDX010000000
VERSION     DAOSDX010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26832_length_1547_cov_3.615282"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..>1547)
                     /locus_tag="PLN02_14295"
     CDS             complement(36..>1547)
                     /locus_tag="PLN02_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HOI53547.1"
                     /translation="GQLRVAQRIASSTLALEGLCLYRDGQRHDHLFLLGKDGQAEQWL
                     LSGGAPRLLRRLALPPGSEHCRGDDARQRLLVSEADFGLWAYDAEGEGVPARHLLAAA
                     RPWGKLSGGAGALAVLPAGVALLDAGGTRLHLWQGRAATRLKVDDGEALAVWGERLLL
                     RDGDGWRVLPTQLAPGQPAASPSLPVLQAVAQTDGVARAGDAADDPAIWIHPGEPARS
                     RVLGTNKKQGLLVYDLQGRQQQLLEAGRLNNVDVRQQVRLGGRVVDLAVATQRDEKAL
                     ALFEIDPDGRLRDVGRVATELREIYGTCLYQPPEGGLEVIANDKDGRFVQYRLELRDG
                     VYAGHPVRRFRLASQPEGCVADDRHGRLFVGEEDVGIWSLAASAAAAAEPVRVMAVGR
                     RLVADVEGLALYHGEKASYLVASSQGNNSYVVLDAQPPHAYRGAFRIGLNPEAGIDGV
                     SETDGLEVTARNLSGPYGAGMLVVQDGYKRLPDGTQNFKYLPWQDVARALGLP"
BASE COUNT          219 a          583 c          542 g          203 t
ORIGIN      
        1 gtcgacgggg ccgggcaggg ccagggcggg atagctcacg gcagccccag cgcccgcgcc
       61 acgtcctgcc agggcaggta cttgaagttc tgggtaccgt cgggcaggcg cttgtagccg
      121 tcctggacga ccagcatgcc agcgccgtag gggccgctga gattgcgcgc ggtgacttcc
      181 aggccgtcgg tctcggatac gccgtcgatc cctgcctcgg gattcaggcc gatgcggaag
      241 gcgccacggt aggcgtgcgg cggctgggcg tcgagaacca cgtagctgtt gttgccctgg
      301 ctcgacgcca ccaggtagct ggccttctcc ccgtggtaga gggccagtcc ttcgacgtcg
      361 gcgaccaggc gccggccgac cgccatcacc cgcaccggct ccgccgccgc tgcggcagac
      421 gccgccaggc tccagatgcc gacatcctcc tcgccgacga agaggcgacc gtggcgatcg
      481 tcggcaacgc agccctcggg ctggctggcc agccggaaac ggcgcaccgg atggccagca
      541 tagacgccgt cgcgcagttc cagccggtac tggacgaaac gcccgtcctt gtcgttggcg
      601 atcacctcca gcccgccttc gggcggctgg tagaggcagg tgccgtagat ttcccgcagt
      661 tccgtcgcca cccggccgac gtcgcgcaag cgcccgtcgg ggtcgatctc gaacagggcg
      721 agcgccttct cgtcgcgctg ggtggccacc gccaggtcca cgacccggcc gccgagacga
      781 acctgctgcc gcacatcgac gttgttcagg cggccggctt cgagcaactg ctgctggcgg
      841 ccctgcaggt cgtagaccag caagccctgt ttcttgttgg tgccgagcac ccgcgagcgg
      901 gccggctcgc cggggtggat ccagatcgcc ggatcgtcgg cggcatcgcc cgcgcgggcg
      961 acgccgtcgg tctgcgccac ggcctgcagc accggcagcg acgggctggc cgccggctgg
     1021 cctggcgcca gctgcgtcgg cagcacccgc cagccgtcgc cgtcgcgcaa cagcaagcgc
     1081 tcgccccaga cggccagggc ttcgccgtca tcgaccttga gccgcgtcgc cgcccgcccc
     1141 tgccagaggt gcaggcgagt gccgccggca tcaagcaggg cgacgccggc gggcaggacg
     1201 gccagcgcgc cggcaccacc ggacagcttg ccccacggcc gggcggccgc cagcaagtgc
     1261 cgcgccggca cgccctcgcc ctcggcgtcg taggcccaga ggccgaaatc agcctcgctg
     1321 acgagcaggc gctggcgcgc atcgtcgccg cgacagtgct cgctccccgg cggcagggcg
     1381 agacggcgca gcagccgcgg cgcgccgccg ctcaacaacc actgctcggc ctggccgtcc
     1441 ttgccgagca ggaacaggtg gtcgtgacgc tggccgtccc ggtacaggca cagcccttcc
     1501 agcgccagcg tcgacgacgc aatgcgctgc gccacccgca gttggcc
//
LOCUS       DAOSDX010000789         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26838_length_1547_cov_3.017426, whole genome shotgun sequence.
ACCESSION   DAOSDX010000789 DAOSDX010000000
VERSION     DAOSDX010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26838_length_1547_cov_3.017426"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLN02_14300"
     CDS             complement(<1..553)
                     /locus_tag="PLN02_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOI53548.1"
                     /translation="MDQALPRLRSKLEFDGPLGSYLGDTRIRLLEAIDQQGSISQAAK
                     AVPLSYKAAWDAVDAMNNLADQPLVLRSTGGKHGGGTQLTDYGRKVIALYRALEAEYQ
                     AALDRLTASMNDGQASDFQQFSQLLKRMSMKTSARNQFAGKVVGLREGNVDFEVRLRL
                     DEENELVAVITGESAEYLGLAIGM"
     gene            731..1159
                     /locus_tag="PLN02_14305"
     CDS             731..1159
                     /locus_tag="PLN02_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOI53549.1"
                     /translation="MNVSARNVFAGKITALTDGAVNAEVELTLSGGDKIVAIVTEGSV
                     QSLGLAVGKDAVAYVKAPWVMLLSGAPDVRFSARNQLAGTVSALTKGAVNTEVGITLA
                     GGTVVHSVVTNEAVLELGLKIGEPATALVKASHVILGVPA"
     gene            complement(1206..>1547)
                     /locus_tag="PLN02_14310"
     CDS             complement(1206..>1547)
                     /locus_tag="PLN02_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI53550.1"
                     /translation="AAFESLPKILPEAARTLGKNEWQIFCYVLLPNIRNALAGGLILA
                     LGRSLGEVGMTMMLGGNIEGRTVTASLDIYNAVTSGDFERAAAVSLLLGLVSSGLFFL
                     LRRNARAPLGW"
BASE COUNT          274 a          470 c          501 g          302 t
ORIGIN      
        1 ccatgccgat ggccagcccg aggtattccg ccgattcgcc ggtgatcacg gcaaccagtt
       61 cgttctcctc gtcgagcctc aggcggacct cgaagtcgac gttgccttcg cgcaggccga
      121 cgaccttgcc ggcgaactgg ttgcgcgcgc tggtcttcat cgacatgcgc ttcaacaact
      181 ggctgaactg ctggaaatcg ctcgcctggc cgtcgttcat gctcgccgtc agacggtcga
      241 gcgccgcctg gtactcggct tcgagcgccc ggtagagggc gatcaccttg cgtccgtaat
      301 cggtcagttg cgtaccgccg ccgtgcttgc cgccggtcga gcgcagcacc agcggttgat
      361 cggccaggtt gttcatcgcg tcgacggcat cccaggccgc cttgtacgac agcggcacgg
      421 cttttgctgc ctgcgagatc gagccttgct ggtcgatcgc ctcgagcagg cggatgcggg
      481 tgtcgcccag atagctgccg agcgggccgt cgaattcgag cttgctgcgc aggcggggaa
      541 gggcttgatc cattatgtta tgtctttcga tatataacga tttcgagttt accggaaaat
      601 tatcgaggcg ctatggttgt ctgttcataa atgggttggt tatattgcca acaagccggg
      661 ttggttacgt tgcttattgt cgtatatagc gaaaagcggg ccagccggat tgataaccaa
      721 ggagaacgcc atgaatgtca gcgcacgcaa tgtttttgca ggaaaaatca ccgccttgac
      781 cgacggtgcg gtcaacgccg aagtcgagct caccttgtcg ggaggcgaca aaattgtcgc
      841 aatcgtcaca gagggcagcg ttcagtcgct cggcctggcg gtcggcaagg acgccgtcgc
      901 ctacgtcaag gcaccgtggg tgatgctgct gtcgggggcg ccggacgttc gcttctcggc
      961 gcgcaaccag ctcgccggca cggtcagcgc gctgaccaag ggcgccgtca ataccgaagt
     1021 cggtatcacg ctggccggcg gtacggtcgt ccattcggtg gtcaccaacg aagccgtgct
     1081 cgaactcggt ctcaagatcg gcgaaccggc gacggcgctg gtcaaggcca gtcacgtcat
     1141 cctcggcgtg ccagcctgaa gcagcggcac gcgccaaggg gcggccgtcc ggccgccttt
     1201 ttcgttcacc agccgagcgg cgcgcgggca ttgcgccgca gcaggaagaa gaggccgctg
     1261 ctgaccagtc cgagcagcag cgagacggcg gcggcgcgtt cgaagtcgcc gctggtcacc
     1321 gcgttgtaga tgtcgagcga ggcggtcacc gtgcgcccct cgatgttgcc gccgagcatc
     1381 atggtcatgc cgacttcgcc cagcgagcgg ccgagggcga ggatcaggcc gccggccagt
     1441 gcgttgcgga tattgggcag cagcacgtag cagaaaatct gccactcatt cttgcctagt
     1501 gtgcgcgccg cttccggcag aattttcggc agactttcga aagccgc
//
LOCUS       DAOSDX010000790         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26853_length_1546_cov_4.665996, whole genome shotgun sequence.
ACCESSION   DAOSDX010000790 DAOSDX010000000
VERSION     DAOSDX010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26853_length_1546_cov_4.665996"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="PLN02_14315"
     CDS             complement(<1..1447)
                     /locus_tag="PLN02_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOI53551.1"
                     /translation="MPLPAFTRSLRFRLVISSLLIELLVLTLLIGNGVRLINHHLGEQ
                     SLRHHAAIQQAYKVAVAVPLATRDYATLRDIFDGWQESEEIVYLALTDPEGHRLASSR
                     WPENQPLPPPGADLQGADIVHIRFPIEIYGQHYGELQYGLSQKFMIAARQELLAQGVL
                     IALAGILLTTLVLTATGYWLTRHLSTLAAASSRIAAGDYRAPLPISETGEIGALSHNF
                     AAMANAVETRIEELAAHLSRQKSILDALGEGVFGIDEQHRCIFVNPAACELLHYLPDD
                     LLGQDTHRLFHHTRPDGSFFPEAECPVHKTVIDGLRRSGNDWFWRRDGSGIPVMLTVN
                     PLWREGRHCGAIVAFSDITDLIGATAALRESNGRLTRFIDALPDIVVIKDGQNRWQQI
                     NHAAEEVFDLHDVPWQGKTNRELADIRPAFRSFHSAAADTDEIAWRENGISHSIEYLN
                     PGDAPVRICEVRKIASRAADGTPQALMVIARD"
BASE COUNT          235 a          461 c          566 g          284 t
ORIGIN      
        1 tgtcgcgggc aatcaccatc agcgcctgcg gcgtgccgtc ggcggcgcgg ctggcgatct
       61 tgcgcacttc gcagatgcgg accggcgcat cacccggatt caggtactcg atgctgtggc
      121 tgatgccgtt ttcgcgccag gcgatctcat cggtgtcggc ggcggcgctg tggaagctgc
      181 ggaaggccgg ccggatatcg gccagttccc ggttggtctt gccctgccag gggacgtcgt
      241 gcaggtcgaa gacttcctcg gcggcatggt tgatctgctg ccagcggttc tgcccgtcct
      301 tgatgacgac gatgtcgggc agcgcgtcga tgaaacgggt cagccggccg ttgctctcgc
      361 gtagcgcggc ggtggcgccg atcaggtcgg tgatgtcgct gaaggcgacg atggcgccgc
      421 agtgccggcc ctcgcgccag agtggattga ccgtcagcat caccgggatg ccgctgccgt
      481 cgcggcgcca gaaccagtcg ttgccgctgc gccgcaagcc gtcgatgacg gtcttgtgca
      541 ccgggcactc ggcttccggg aagaaactgc cgtcgggccg ggtgtggtgg aagaggcggt
      601 gcgtgtcctg accgagcagg tcgtcgggca ggtagtgcag gagttcgcag gcggccggat
      661 tgacgaagat gcagcgatgc tgttcgtcga tcccgaaaac gccttcgccg agcgcgtcga
      721 ggatcgattt ctggcggctc aggtgggcgg ccagttcctc gatccgggtt tcgacggcat
      781 tcgccatcgc cgcgaaattg tggctgaggg cgccgatctc accggtttcg ctgatcggca
      841 gcggcgcccg gtaatcgccg gcggcaatcc ggctgctggc cgcggccagc gtgctcaggt
      901 gacgggtcag ccagtagccg gtggcggtca gcaccagcgt cgtcagcagg atgccggcga
      961 gggcgatcag cacgccctgg gcgagcagct cctggcgggc ggcgatcatg aacttctgcg
     1021 acaggccgta ctgaagttcg ccgtaatgct ggccgtaaat ttcgatcggg aaacggatat
     1081 ggacgatgtc ggcgccctgc aggtcggcgc cgggcggtgg caggggctgg ttttccggcc
     1141 agcggctgct ggccagccgg tggccttcgg ggtcggtcag ggcgaggtag acgatttcct
     1201 cgctttcctg ccagccatcg aaaatatcgc gcagcgtcgc gtagtcgcgg gttgccagcg
     1261 gcactgcgac ggcgaccttg taggcctgct ggatggcggc gtgatggcgc agcgactgct
     1321 cgccgaggtg gtggttgatc agccgcactc cgttgccgat caacagcgtc aacaccagga
     1381 gttcgatcag caggctgctg atcaccaggc ggaaccgcag gctgcgcgtg aaggcaggca
     1441 ggggcaaggg cttacctcat catctgcacg gtcagatcga cgaagggacg cagcttctcg
     1501 atgtcgtctg gcgtcacggc gacatagccg ttgtagccgg tgtcgc
//
LOCUS       DAOSDX010000791         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26854_length_1546_cov_4.665325, whole genome shotgun sequence.
ACCESSION   DAOSDX010000791 DAOSDX010000000
VERSION     DAOSDX010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26854_length_1546_cov_4.665325"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLN02_14320"
     CDS             complement(<1..589)
                     /locus_tag="PLN02_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53552.1"
                     /translation="MKGFKRNLLFAAVVSALGGAAHAASTIDMVVAIDESGSMSGEHN
                     QFIGSYIENLDNVLNAQSVTVNRYGLGGFGGGSYQGTSANEAGRETSSSALYRHLDLS
                     SGVDAVWGDAAEFKLVTPQLDTSGGTEDGYRMIDYVLRNYQFRNNAGASILLITDEDR
                     DVDTGGTPVTSGLPQGQTIYDTNTANNTATMQQMLA"
     gene            811..1179
                     /locus_tag="PLN02_14325"
                     /pseudo
     CDS             811..1179
                     /locus_tag="PLN02_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(1195..>1546)
                     /locus_tag="PLN02_14330"
     CDS             complement(1195..>1546)
                     /locus_tag="PLN02_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOI53553.1"
                     /translation="VVWSVIVGSLDNFLRPMLIRRGADLPLLLIFAGVIGGMLGFGLI
                     GIFVGPVVLAITWTLLQAWIADALGDESAADQANAAEPAAGESGAVVAQGEAGAGVEQ
                     VLENHVKPGGDTPR"
BASE COUNT          296 a          526 c          439 g          285 t
ORIGIN      
        1 gcgccagcat ctgctgcatc gttgcggtat tgttggccgt gttcgtatcg tagatcgtct
       61 gcccttgcgg caatccgctg gtaaccggcg taccgccagt atcgacatcg cgatcctcgt
      121 cggtaatcag cagaatcgat gctccggcgt tgttacggaa ctgatagttg cgcaacacgt
      181 aatcgatcat ccggtaaccg tcttcggtac cgccagaagt atctagctgc ggcgtcacca
      241 gtttgaactc ggcagcatcc ccccacacgg catcgacacc gctggacagg tcaaggtgcc
      301 ggtaaagcgc gctactactg gtttcgcgcc cggcttcatt ggccgaggtc ccctgatatg
      361 aacccccacc gaagccaccc agaccataac ggttgaccgt cacactctgg gcattgagca
      421 cgttgtcgag gttttcgatg tacgagccga tgaactggtt gtgctctccg gacatcgagc
      481 cgctttcgtc aatcgcgacg accatatcga tagtgctggc ggcatgcgct gcgccgccca
      541 atgccgaaac cactgctgca aaaagcagat tacgcttgaa acccttcatg aaaactcctt
      601 gatcaatgca aaatgtcgcc accagcgggc aacggacccg ccggattttt gcatcaattt
      661 acatctcgaa caagatagac caaagacata accgggaaat gccgatcaaa taccccgcct
      721 cgcagaatga ccgccacaag ctcacttaaa cgcagctacc gcctgatcgc acctttctat
      781 gacctggccg tggagcaggc gacgcgcggc atgcgccagc acagcctgca cggtctgcct
      841 tcgcggccgg gcaccatcct gctcgccggt gtcggcaccg gcctcgacct gccccatctg
      901 ccaccgcagc accgctacgt cggtatcgac ctgaccggcg ccatgctcag gcgcagcctg
      961 ccgcgcgccg gccggcttga tttccgtccg gtgcagggag acgtgcatca cctgccgttt
     1021 gccgacgcca gtttcgatgc cgtcgtcgcc cacctgatcc tggccgtggt gccgcaaccg
     1081 gcgctgtgcc tggcggaaat ttcgcgggta ttgaagccgg gcggcgaagc gctgatcctc
     1141 gacaagttcc tgcgccgcca gcagaaggcg ccgttgcggc gcctgctcaa tccgctagcg
     1201 cggcgtatcg ccacccggtt tgacgtggtt ttcgaggact tgctcgacgc cagccccggc
     1261 ctcgccctgc gcgacgacag ccccgctttc gccggcggct ggttccgccg cattcgcctg
     1321 atccgccgct gactcgtcgc cgagggcgtc ggcaatccac gcctgcagca gcgtccaggt
     1381 gatggccagg accaccggtc cgacgaagat gccgatcagg ccgaagccga gcatgccgcc
     1441 gatgacgccg gcgaagatca gcagcaaggg cagatcggcg ccgcgccgga tcagcatcgg
     1501 gcgcaggaag ttgtcgaggc tgccgacgat cacgctccac accacc
//
LOCUS       DAOSDX010000792         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26912_length_1543_cov_3.463710, whole genome shotgun sequence.
ACCESSION   DAOSDX010000792 DAOSDX010000000
VERSION     DAOSDX010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26912_length_1543_cov_3.463710"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PLN02_14335"
     CDS             <1..163
                     /locus_tag="PLN02_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOI53554.1"
                     /translation="AVRVYYKPYVDWIWGGCAIMALGGLLAILDRRYRSRRKPAADVA
                     ANVLGGNQG"
     gene            160..744
                     /locus_tag="PLN02_14340"
     CDS             160..744
                     /locus_tag="PLN02_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HOI53555.1"
                     /translation="MNRYYWILGAFAALVVLLAVGLNLNPREVPSPLVGKPAPAFRLA
                     VLGKEGATMGPEDMKGKVWLLNVWSTWCVSCRQEHPVLVEFSRNGQVPVVGLNYKELR
                     GDGEIDMRRVPPEQEVPLAVKRAQGWLTEHGNPYLLTVMDIDGRVGIDYGVYGVPETY
                     VIDKTGVIRMKHTGPISPQVLKDKILPLVAELNR"
     gene            741..1220
                     /locus_tag="PLN02_14345"
     CDS             741..1220
                     /locus_tag="PLN02_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOI53556.1"
                     /translation="MNCARILFLVLSLLSTAAYASRTNEAAVADDPVAEKRLQELSKE
                     LRCLVCQNETIADSNAELAVDLRREIRGMIHDGKSNAEILDFMVSRYGDFVLYRPPVK
                     GITMLLWGGPLALLLIAILAMRSYLKRRSQQTAAQAKPLTAEEIRRAEALLKENEEK"
     gene            1217..>1543
                     /gene="ccmI"
                     /locus_tag="PLN02_14350"
     CDS             1217..>1543
                     /gene="ccmI"
                     /locus_tag="PLN02_14350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HOI53557.1"
                     /translation="MTQFALFATLLVVVTALLIIPGLWFGRRRPSDADRKAANLAIFR
                     DQLADLERERLEGNLGEADFAQAKVELQKRLLEEVGETQESDGGPATSRKTALLVLFL
                     LPLAAVG"
BASE COUNT          268 a          501 c          506 g          268 t
ORIGIN      
        1 ggcggtgcgc gtctattaca agccctatgt cgactggatc tggggcggct gcgcgatcat
       61 ggcactgggc ggcttgctgg ccatccttga ccgccgctac cgcagccggc gcaagcccgc
      121 agctgatgtc gccgcgaacg tgcttggggg aaatcaagga tgaatcgtta ttactggata
      181 cttggcgcct ttgccgcgct ggtcgtgctg ctcgccgtcg gtctcaacct gaatccgcgc
      241 gaggtgccgt cgccgctcgt cggcaagccg gcgccggcgt tccgcctggc cgtcctgggc
      301 aaggagggcg cgacgatggg gccggaagac atgaagggca aggtctggct gctcaatgtc
      361 tggtctacct ggtgcgtctc ctgccgccag gaacacccgg tgctcgtcga gttctcgcgc
      421 aacggccagg tgccggtggt cggcctgaac tacaaggaac tgcgcggcga cggcgagatc
      481 gacatgcgcc gcgtgccgcc cgagcaggaa gtgccgctgg ccgtcaaacg ggcgcagggc
      541 tggctgaccg agcatggcaa tccttacctg ctgacagtga tggacatcga cggccgggtc
      601 ggcatcgatt acggcgtcta tggcgtcccg gaaacctacg tcatcgacaa gacgggcgtg
      661 atccgcatga agcataccgg gccgatcagc ccgcaggtgc tcaaggacaa gatcctgccc
      721 ctggtcgcgg agctgaatcg atgaattgcg cccgtatcct gttcctggtc ctcagcctgc
      781 tgtcgacggc ggcctacgcc tcgcgcacca acgaagcggc ggtggccgac gacccggtcg
      841 ccgaaaagcg cctgcaggag ttgtccaagg agctgcgttg cctggtctgc cagaacgaga
      901 cgatcgccga ctccaacgcc gagctggccg tcgatctgcg gcgggaaatc cgcggcatga
      961 tccatgacgg caaatccaac gccgagattc tcgatttcat ggtcagccgt tacggcgatt
     1021 tcgtgctcta ccggccgccg gtcaagggca tcacgatgct gctctggggc ggtccgctgg
     1081 ccctgctgct gatcgccatc ctggccatgc gctcctatct caagcggcgc agccagcaga
     1141 ccgccgccca ggccaagccg ctgacggccg aggaaatccg ccgcgccgaa gcgttgctca
     1201 aggaaaacga agagaaatga cccagttcgc cctttttgcc accctgctcg tcgtcgtcac
     1261 ggcgctgctg atcattcccg gtctctggtt cgggcgtcgt cgccccagcg acgccgaccg
     1321 caaggcagcc aacctggcaa tcttccgcga tcaactggcc gacctcgaac gcgaacgtct
     1381 cgagggcaat cttggcgagg ccgattttgc gcaggccaag gtggagctgc agaagcgcct
     1441 gcttgaggaa gtgggcgaaa cccaggagag cgatggcggg ccggccacca gccgcaagac
     1501 tgcgctgctg gtgctgttcc tgctgccgct ggcggccgtc ggc
//
LOCUS       DAOSDX010000793         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26972_length_1540_cov_3.397306, whole genome shotgun sequence.
ACCESSION   DAOSDX010000793 DAOSDX010000000
VERSION     DAOSDX010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26972_length_1540_cov_3.397306"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLN02_14355"
     CDS             <1..847
                     /locus_tag="PLN02_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOI53558.1"
                     /translation="RPPAGSIPSNFLALSPRTRHAIAIDTSRSRLYLFENRPQGLQLI
                     ADYYISVGKLGVDKAAEGDQRTPLGVYYITSNLDPRTLKDFYGAGALPINYPNPLDQL
                     RGKTGSGIWLHGTQSDTFSRPPLASDGCVVLTNQDLDAIRSNLQVGLTPVIISDSIEW
                     LSLDDWAKEREALNQTIEAWRTDWESRDSARYLNHYSRQFSAGGQNFAQFAEQKRSVN
                     AGKEWIKVRLDNLSVFRNPGKEELIVVTFDQDYRSNNLNNRMKKRQYWVREGGRWKII
                     YEGAA"
     gene            849..1409
                     /locus_tag="PLN02_14360"
     CDS             849..1409
                     /locus_tag="PLN02_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI53559.1"
                     /translation="MKKLTGILAALTLCSTLAQAAPTLELQTSLGRIVVELDAQKAPK
                     SSANFLQYAKDGFYNGTVFHRVIPGFMIQGGGFDAGMSQKATRAPIENEAKNGLRNRR
                     GTLAMARTMDPHSATAQFFINHADNAPLDYPGSDGWGYAVFGKVTEGMDVVDKIASQP
                     TTSKGFHQDVPRTPVVIQNVKILSEK"
     gene            1421..>1540
                     /locus_tag="PLN02_14365"
     CDS             1421..>1540
                     /locus_tag="PLN02_14365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOI53560.1"
                     /translation="MIKLTTNFGVISLELDAEKAPKTVENFIAYVEAGHYDNTV"
BASE COUNT          340 a          556 c          421 g          223 t
ORIGIN      
        1 gcgcccgccg gccggcagca tcccgagcaa cttcctcgcg ctgtcgccgc gcacgcgcca
       61 cgccatcgcc atcgacacct cacgctcgcg cctctacctg ttcgagaacc gcccacaagg
      121 cctgcagctc atcgccgact actacatctc ggtcggcaag ctcggcgtgg acaaggccgc
      181 cgaaggcgac cagcgcactc cactgggcgt ctactacatc accagcaacc tcgatccgcg
      241 caccctcaag gacttctacg gcgccggcgc cctgcccatc aactacccca acccgctgga
      301 ccagctgcgc ggcaagaccg gcagcggcat ctggctgcac ggcacgcaat ccgacacgtt
      361 ctcgcgaccg ccgctcgctt cagatggctg cgtcgtgctg accaaccagg acctcgacgc
      421 catccgcagc aacctgcagg tcggactgac gccggtgatc atcagcgaca gcatcgagtg
      481 gctgtcgctc gacgactggg ccaaggagcg tgaagccctc aaccagacga tcgaggcctg
      541 gcgcaccgac tgggagagcc gcgacagcgc gcgctacctc aaccactact cgcgtcagtt
      601 cagcgccggc ggccagaatt tcgcgcagtt cgccgaacag aagcgctcgg tcaacgctgg
      661 caaggaatgg atcaaggtcc gcctcgacaa tctttcggtg ttccgcaatc cgggcaagga
      721 agaactgatc gtcgtcacct tcgaccagga ttaccgcagc aacaacctca acaaccggat
      781 gaagaaacgc cagtactggg tacgcgaagg cggccgctgg aagatcatct acgaaggagc
      841 cgcctgacat gaagaaactg actggcattc tcgccgccct gacgctgtgc tcgacgctcg
      901 cccaggccgc cccaaccctg gaactgcaga ccagcctcgg ccgcatcgtc gtcgaactag
      961 acgcgcagaa ggcaccgaaa agcagcgcca acttcctgca atatgcgaag gacggtttct
     1021 acaacggcac cgtcttccac cgcgtgatcc ccggcttcat gatccagggc ggcggcttcg
     1081 acgctggcat gtcgcagaaa gctacccgcg cgccgatcga gaacgaggcg aagaacggtc
     1141 tcaggaaccg ccgcggcacg ctggccatgg cccgtaccat ggacccacac tcggcgaccg
     1201 cgcagttctt catcaaccac gccgacaatg ccccgctcga ctacccgggc agcgatggct
     1261 ggggctatgc cgtcttcggc aaggtcaccg agggcatgga cgtggtcgac aagatcgcca
     1321 gccagcccac caccagcaag ggcttccacc aggacgtgcc gcgcacgccg gtggtcatcc
     1381 agaacgtcaa gattctctcc gaaaaataag gaactccccc atgatcaagc tcaccaccaa
     1441 cttcggcgtc atcagcctcg aactcgacgc cgagaaggcg ccgaaaaccg tcgagaactt
     1501 cattgcctac gtcgaagccg ggcattacga caacaccgtc
//
LOCUS       DAOSDX010000794         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_26997_length_1539_cov_2.910377, whole genome shotgun sequence.
ACCESSION   DAOSDX010000794 DAOSDX010000000
VERSION     DAOSDX010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26997_length_1539_cov_2.910377"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..>1539
                     /locus_tag="PLN02_14370"
     CDS             213..>1539
                     /locus_tag="PLN02_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOI53561.1"
                     /translation="MSILKSQLNPRSADFQANATTMRAVVADLREKVEKIALGGPEAA
                     RQKHLARGKLLPRERVSALLDPGTPFLEIGQFAAYGMYDGDVPAASVIAGIGRVEGVE
                     CMIVANDATVKGGTYYPLTVKKHLRAQEIALENRLPCIYLVDSGGAFLPMQDEVFPDK
                     EHFGRIFFNQANLSAAGIPQIAAVMGSCTAGGAYVPAMCDESIIVRDQGTIFLGGPPL
                     VKAATGEVVTAEDLGGADVHTRISGVADHLAENDAHALAIARRIVRDLNWKKAPSVTL
                     TAPSAPLFPAEELYGVIPTDTKKPFDVREIIARVVDGSDFDEFKARYGTTLVCGFARI
                     YGYPVGIVANNGILFSESALKGAHFIELCAQRGIPLVFLQNITGFMVGRKYENGGIAR
                     DGAKMVTAVATAKVPKFTVVIGGSFGAGNYGMCGRAYSPRFLWMWPNARI"
BASE COUNT          294 a          536 c          468 g          241 t
ORIGIN      
        1 ggcgatgttc gatcacgccg cggcagaagg cttcctgcgc gaacagaacc gggcgatggc
       61 gctcagcgac agcgaccccg aagcgctgct ggcggcgatg gcagccttcc gccccgaacc
      121 ggtcagcaaa tggctcaagg aggaaaggca gttgtaaagc cttttccgtt tttcattttc
      181 aggcaacgca ccaataaaag aagcgaggag acatgagcat cctgaaatcc caactgaatc
      241 cacgttcggc cgacttccag gccaacgcga cgaccatgcg ggcggtcgtc gccgacctcc
      301 gtgagaaagt cgagaagatc gccctcggcg gcccggaagc ggcgcgccag aagcacctgg
      361 cgcgcggcaa gctgctgccg cgcgaacggg tcagtgccct gctcgatccc ggcacgccct
      421 tcctcgaaat aggacaattc gcggcgtacg ggatgtacga cggcgacgtg ccggcagcgt
      481 cggtaatcgc cggcatcggc cgcgtcgaag gcgtcgaatg catgatcgtc gccaacgacg
      541 cgacagtgaa gggcggcacc tactacccgc tgaccgtcaa gaagcacctg cgcgcccagg
      601 aaatcgccct ggaaaaccgc ctgccctgca tctatctggt ggattccggc ggcgccttcc
      661 tgcccatgca ggacgaggtc ttcccggaca aggagcactt cggccgcatc ttcttcaacc
      721 aggccaacct gtcggccgcc ggcatcccgc agatcgccgc cgtcatgggt tcatgcacgg
      781 ccggtggcgc ctatgtgccg gcgatgtgcg acgagtcgat catcgtccgc gaccagggca
      841 cgatctttct cggcggcccg ccgctggtca aggcggcgac cggcgaggtc gtcacagccg
      901 aagacctcgg cggcgccgac gtgcatacgc gcatctccgg cgtcgccgac cacctggccg
      961 agaacgacgc gcacgcgctg gccatcgcgc ggcgcatcgt gcgcgacctg aactggaaaa
     1021 aagccccgtc agtgacgttg accgcgccca gcgccccgct attccccgcc gaagagctgt
     1081 acggcgtgat cccgaccgac accaagaagc ccttcgacgt gcgcgaaatc atcgcccgcg
     1141 ttgtcgacgg ctccgatttc gacgagttca aggcacgcta cggcaccacg ctggtctgcg
     1201 gcttcgcgcg catctacggc tacccggtcg gcatcgtcgc caataacggc atcctgttca
     1261 gcgagtcggc cctcaagggc gcgcatttca tcgaactctg cgcccagcgc ggcatcccgc
     1321 tcgtcttcct gcagaacatc accggcttca tggtcggccg caaatacgaa aacggcggca
     1381 tcgcccgcga cggcgccaag atggtcaccg ccgtcgcgac cgcgaaagta ccgaaattca
     1441 ctgtcgtcat cggcggcagc ttcggcgccg gcaattacgg catgtgcggc cgcgcctact
     1501 cgccccgctt cctgtggatg tggccgaacg cgcgcatct
//
LOCUS       DAOSDX010000795         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27000_length_1539_cov_2.652965, whole genome shotgun sequence.
ACCESSION   DAOSDX010000795 DAOSDX010000000
VERSION     DAOSDX010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27000_length_1539_cov_2.652965"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLN02_14375"
     CDS             complement(<1..211)
                     /locus_tag="PLN02_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase"
                     /protein_id="HOI53562.1"
                     /translation="MSKALRWSERWFRFGLWLVALAFGSFLIGLGGNIVENLPRVEAA
                     LSLENFLDPAAAGKLRAEIDAARLAR"
     gene            300..812
                     /locus_tag="PLN02_14380"
     CDS             300..812
                     /locus_tag="PLN02_14380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOI53563.1"
                     /translation="MPETVFHLRPAQAADLAAILAVQRQCYLPSMNEDGATWAGRLAA
                     AAGFVWVGEQAGEIGAYLATYPSQLGKVTPLGGAFALAAGADCLYLHDLAVAPGAAGR
                     GLGARLVACALAAARRHGWRHAALVCVQDAYTFWLRQGFSEPATLAPAAQAALASYPG
                     PARYMTRPLD"
     gene            complement(816..1469)
                     /gene="nfsB"
                     /locus_tag="PLN02_14385"
     CDS             complement(816..1469)
                     /gene="nfsB"
                     /locus_tag="PLN02_14385"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HOI53564.1"
                     /translation="MNVAEIATTRRTCKAFDPTRKVDPAAIEQLKTLLRFAPSSVNSQ
                     PWHFIVASSAEGKEKIAATLAGRFEYNAPKVRNASHVVVLCVRRDLDDAHLQALADQE
                     DADGRFATAEAKATQHNARSFYTNLHRDQLNDLPRWTEKQIYIALGALLFGAAALGID
                     ACPMEGIDAAAIDAALDLEAQGLRCVVLTALGYSSGDDFNARLPKSRLPAAAVISEI"
BASE COUNT          223 a          536 c          529 g          251 t
ORIGIN      
        1 ggcgggcgag ccgggcggca tcgatctcgg cgcgcagctt gccggcggcg gccgggtcga
       61 gaaagttttc caggctcaac gccgcttcga cgcgcggcag gttctcgacg atgttgccgc
      121 cgagcccgat caggaaactg ccgaaagcca gggcaaccag ccacaatcca aagcggaacc
      181 agcgttccga ccagcgcaat gccttgctca tcgtcttcct ccccgcaagt ggcgacgggt
      241 cgatgatacg cgcctgacgg ggaattgact tcgccctgcc gctgcctaca atcgccgcca
      301 tgccggaaac cgtcttccac ctgcgtcccg cgcaagccgc cgatctcgcc gccatcctcg
      361 ccgtgcagcg ccagtgctac ctgccgtcga tgaacgagga cggcgccacc tgggccggcc
      421 gtctggccgc tgccgccggt ttcgtctggg tcggcgaaca agccggcgaa atcggcgcct
      481 acctcgccac ctacccgtcg cagctcggca aggtgacgcc gctgggtggc gctttcgcgc
      541 tcgccgccgg cgccgactgc ctctacctgc acgacctcgc ggtagcgccg ggcgccgccg
      601 gccgcggtct cggcgcgcgc ctggtcgctt gcgcactggc cgccgcccgc cgccacggct
      661 ggcggcacgc cgcgctggtc tgtgtgcagg acgcctatac cttctggctg cgccagggct
      721 tcagcgaacc ggcgacgctg gcgccggccg cgcaggcggc gctcgccagc tatcccggtc
      781 cggcgcgcta catgacgcgc ccgctcgact gagcctcaga tctcgctgat caccgccgcc
      841 gccggcaggc gcgacttcgg cagccgggcg ttgaaatcgt cgccactgct gtaaccgagc
      901 gcggtcaaca cgacgcagcg cagcccctgc gcctccaggt cgagcgcggc gtcgatcgcc
      961 gccgcgtcga tgccttccat cgggcaggcg tcgatgccga gcgcggcggc gccgaaaagc
     1021 agcgctccga gcgcgatgta gatctgcttt tcggtccacc gcggcagatc gttcaactgg
     1081 tcgcggtgca ggttggtgta gaagctgcgc gcgttgtgct gggtcgcctt cgcctcggcg
     1141 gtggcaaagc ggccgtcggc atcttcctgg tcggccagcg cctgcaggtg ggcgtcgtcg
     1201 agatcgcggc ggacacagag gacgaccacg tgcgacgcgt tgcgcacctt gggcgcgttg
     1261 tactcgaagc ggccggccag cgtcgcggca atcttctcct tgccttcggc gctgctggcg
     1321 acgatgaagt gccagggctg cgaattgacc gacgacgggg cgaagcgcag cagcgtcttc
     1381 aactgttcga tggcggcggg atcgaccttg cgggtcggat cgaaggcctt gcaggtgcgg
     1441 cgggtggtgg cgatttcggc aacgttcatg aagggttctc caatggggga cgggacttgg
     1501 tcagcgagct tagcaggaag ttcgccgctt cagatcagg
//
LOCUS       DAOSDX010000796         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27007_length_1539_cov_2.210916, whole genome shotgun sequence.
ACCESSION   DAOSDX010000796 DAOSDX010000000
VERSION     DAOSDX010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27007_length_1539_cov_2.210916"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLN02_14390"
     CDS             <1..484
                     /locus_tag="PLN02_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOI53565.1"
                     /translation="VVGLVVPTGYSFNLDGTSIYLTMAAVFIAQATNTPLDLPHQITL
                     LLILLLTSKGAAGVTGSGFIVLAATLSAVGTVPVAGLALILGIDRFMSEARALTNFVG
                     NSVATLVVAKWCGALDQERLRAVLNNETVDEADHPELVLDDAPAPDIAHTPQPATAHH
                     "
     gene            653..>1539
                     /locus_tag="PLN02_14395"
     CDS             653..>1539
                     /locus_tag="PLN02_14395"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOI53566.1"
                     /translation="MTSSNHSLTPDAAGDIQWLASLSQDFAASLSMPETLDNAIAQIM
                     SYLEVEAASIFLLDPGEATLTCLACAGAVDIRGLTVANGQGIVGRAVSSGQPQLVRDV
                     ATDGDFSGAIDALTGFRTRSILCAPLQVKGRMLGALEVINKRAADANFDAGDCQLLEA
                     LAGVVSLANARMAEALLEQERMRRDLDLAREIQRGLLPQSPPGFPVCAINLPALEVSG
                     DFYDFFPLPDGRIVWGLGDVSGKGMNAALLMAKTISLFRCLGKREPDIGRLLADLNDE
                     IAETALFGMFVTMVLGTYDP"
BASE COUNT          224 a          581 c          476 g          258 t
ORIGIN      
        1 ggtggtcggc ctggtcgtcc ccaccggcta ttcgttcaac ctcgacggca cctcgatcta
       61 cctgacgatg gcggcggtct tcatcgccca ggcgaccaac acgccgctcg acctgccgca
      121 ccagatcacc ctgctgctga tcctgctgct cacctcgaag ggcgccgccg gcgtcaccgg
      181 cagcggcttc atcgtgctcg cggcgacgct ctccgccgtc ggcaccgtgc cggtcgccgg
      241 cctggcgctg atcctcggca tcgaccgctt catgtcggaa gcccgcgcgc tgaccaactt
      301 cgtcggcaac agcgtcgcca cgctggtcgt cgccaagtgg tgcggcgcgc tcgaccagga
      361 gcgcttgcgc gccgtcctca acaacgagac ggtcgacgag gccgaccatc ccgaactggt
      421 cctcgacgac gcgccggcgc cggacatcgc gcacacgccg cagccagcca ccgcccacca
      481 ctgagcgacc aacgatctcc tccagccgct gtgctgcttg cgccccgtcc ctaccgacgg
      541 ggcgtttttt cgtctgccgc cgcgccaagc cggcttgagc caggcggcgc ttgggtttac
      601 attggtgccg actgcctatg cggtcatccc gatgtcctcc gagcgacccg ccgtgacttc
      661 cagcaaccat tccctgacgc ccgacgccgc cggcgacatc cagtggctgg ccagcctcag
      721 ccaggatttc gccgcctcgc tgtcgatgcc cgagacgctc gacaacgcca tcgcccagat
      781 catgagctac ctcgaggtcg aggcggcgtc gatcttcctg cttgatcccg gcgaggcgac
      841 gctgacctgc ctggcctgcg ccggtgccgt cgacatccgc ggcctgaccg tcgccaacgg
      901 ccagggcatc gtcggccgcg cagtgagcag cggccagccg caactggtgc gcgacgttgc
      961 caccgacggc gacttttccg gggcgatcga cgccctgacc ggtttccgca cccgttccat
     1021 cctgtgcgcg ccgctgcagg tcaagggccg catgctcggg gcgctggaag tgatcaacaa
     1081 gcgcgccgcc gacgccaact tcgacgccgg cgactgccag ttgctggagg cgctggccgg
     1141 cgtcgtctcg ctcgccaatg cgcggatggc cgaagcgctg ctcgaacagg agcggatgcg
     1201 gcgcgacctc gacctcgccc gggaaatcca gcgcggcctg ctgccgcagt cgccgcccgg
     1261 ttttccggtg tgcgcgatca acctgccggc gctcgaggtt tccggcgatt tctacgattt
     1321 ctttccgctg cccgacggcc gcatcgtctg gggcctgggc gacgtctcgg gcaagggcat
     1381 gaacgccgcg ctactgatgg ccaagacgat cagcctgttc cgctgcctgg gcaagcgcga
     1441 gcccgacatc ggccgcctgc tcgccgacct caacgacgag attgccgaaa ccgcgctgtt
     1501 cggcatgttc gtcaccatgg tactgggtac ttacgaccc
//
LOCUS       DAOSDX010000797         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27020_length_1538_cov_3.472016, whole genome shotgun sequence.
ACCESSION   DAOSDX010000797 DAOSDX010000000
VERSION     DAOSDX010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27020_length_1538_cov_3.472016"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1359
                     /locus_tag="PLN02_14400"
     CDS             7..1359
                     /locus_tag="PLN02_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOI53567.1"
                     /translation="MSVTRSERRPRGELNRVLVVDDEADIRELIDLTLARMGLAAECA
                     GSVGEAKAALAAGDFQLCLTDMRLPDGEGLEIVRYIAENHPRLPVAVITAWGSAENAV
                     AALKAGAFDYLSKPVGLEQLRALIRSALRLPGNGVENDALRALTGESASMQQVRAMIE
                     KMARSQAPIYISGESGSGKELAARLIHARSARSAGPFVPVNCGAIPENLMESEFFGYR
                     KGAFTGADSDRDGFFQAASGGTLFLDEVADLPLAMQVKLLRAIQEKRVRKVGCVTEEP
                     VDLRIICATHRNLRERVDQGSFRQDLYYRLNVIELRMPPLRERMEDIAPLVDSILNKL
                     CGEAHPRLSPAAIKALQLYSFPGNVRELENILERATALCAGELIEPDDLHLEPEEGAG
                     SDLQGRGSETLDDYLNRLERQAILEALQKTEGNRTAAARLLGVTFRSLRYRLERLGIE
                     "
     gene            1356..>1538
                     /locus_tag="PLN02_14405"
     CDS             1356..>1538
                     /locus_tag="PLN02_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HOI53568.1"
                     /translation="MNWLADGWLDTAARRESPNFGERPAGAEVSLLVVHNISLPPGEF
                     GGDWVEDFFLNRLDPAA"
BASE COUNT          247 a          532 c          524 g          235 t
ORIGIN      
        1 tgattcgtgt ccgttacccg tagcgagcgg cgtccgcgcg gcgagttgaa ccgggtcctc
       61 gtcgtcgacg acgaggccga catccgcgaa ctgatcgatc tgaccctggc ccgcatgggc
      121 ctggcggcgg agtgcgccgg ctcggtcggc gaggcgaagg cggcgctggc ggccggcgac
      181 ttccagttgt gcctgaccga catgcgcctg cccgacggcg aaggtctgga aatcgtccgt
      241 tacatcgccg agaatcatcc gcgcctgccg gtcgccgtga tcaccgcctg gggcagcgcc
      301 gagaacgccg tcgcggcgct caaggccggt gccttcgatt acctgtcgaa gccggtcggc
      361 ctcgagcaat tgcgggcgct gatccgttcc gccttgcgtc tgcccggcaa cggcgtcgag
      421 aacgacgcgc tgcgcgcgct gaccggcgaa tcggcgagca tgcagcaggt gcgggcgatg
      481 atcgagaaga tggcgcgcag ccaggcgccg atctacatct ccggcgaatc cggctccggc
      541 aaggagctgg cggcgcgcct gatccacgcc cgcagcgcgc gcagcgccgg gcccttcgtc
      601 ccggtcaact gcggggcgat tcccgagaac ctgatggaga gcgagttctt cggctatcgc
      661 aagggcgcct tcaccggtgc cgacagcgac cgcgacggct tcttccaggc cgccagcggc
      721 ggcacgctgt tcctcgacga ggtcgccgac ttgccgctgg caatgcaggt caagctgctg
      781 cgggcgatcc aggaaaaacg cgtgcgcaag gtcggctgcg tcaccgagga gccggtcgac
      841 ctgcgcatca tctgcgctac ccaccgcaat ctgcgcgagc gggtcgatca gggcagcttc
      901 cgccaggacc tctattaccg gctgaacgtc atcgaactgc gcatgccgcc cctgcgcgag
      961 cgcatggaag acattgcgcc gctggtcgac agcatcctca acaagctgtg cggcgaggcg
     1021 catccgcggc tgtcgccggc ggcgatcaag gcgctgcagc tctactcctt ccccggcaac
     1081 gtgcgcgaac tggagaacat cctcgaacgg gcgaccgcgc tctgcgctgg cgagttgatc
     1141 gagcccgacg acctccacct tgagcccgag gagggggccg gcagcgatct ccagggacgc
     1201 ggcagcgaga cgctcgacga ctacctcaac cgcctcgaac gtcaggccat cctcgaagcg
     1261 ctgcagaaaa ccgagggcaa ccgcacggcg gcggcgcgcc tgctcggcgt caccttccgt
     1321 tcgctgcgct accggctgga acggctgggc atcgaatgaa ctggctggcg gacggctggc
     1381 tggacacggc cgcacgccgc gagtcgccga atttcggcga gcgtccggcc ggcgccgagg
     1441 tttcgctgct ggtcgtgcac aacatcagcc tgccgcccgg cgagttcggc ggcgactggg
     1501 tcgaggattt ctttctcaac cggctcgatc ccgcggcc
//
LOCUS       DAOSDX010000798         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27058_length_1537_cov_2.651822, whole genome shotgun sequence.
ACCESSION   DAOSDX010000798 DAOSDX010000000
VERSION     DAOSDX010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27058_length_1537_cov_2.651822"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLN02_14410"
     CDS             <1..554
                     /locus_tag="PLN02_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOI53569.1"
                     /translation="PIAGALRMSLPGFVLASAAGATLWAGLALVAGWVLRSEVQAVIA
                     ALDRNAAGALLVVAAVIGLWLGWKLWQKYRFRRLSAVPHISIGELLAALDSNQPPLVL
                     DLRGAIMVAETGPIPGAVVAEHDRLFEAVGDWPRHQPIVTLCHCPEDAGAIQAANRLL
                     DAGYRSVRPLLGGYDAWRAGKAC"
     gene            complement(556..>1537)
                     /locus_tag="PLN02_14415"
     CDS             complement(556..>1537)
                     /locus_tag="PLN02_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOI53570.1"
                     /translation="GVPDAPAEQLAAAQAYPERPLETGKAWKEMLHRYLAGSLGLLVL
                     TLCVLAWRRECRQQQSPALPTALLLIVCLQAALGMWTVTYLLKPVIVTLHLLGGMATL
                     SLLAGMLFARRETSGAAPASPALRLLAGGALLLIVLQIALGGWVSSNYAALACADFPT
                     CQGQWRPEMEFDHAFSLHRELGQTADGQLLPLASLTAIHWSHRLGALAVTLIVGALAI
                     SLIRTGHRRWRRWGGLLLGLLVAQIGLGLGNVLLSLPLPVAVLHTLGAAALLTATLAI
                     NILLQRSPSPAGSGWSPSGKTRSLGARASANLRREDQTPQTAATSVQQRF"
BASE COUNT          222 a          532 c          560 g          223 t
ORIGIN      
        1 cgccgattgc cggcgcgctg cgcatgagcc tgcccggctt cgtgctcgct tctgccgccg
       61 gcgccacctt gtgggccggc ctggcgctgg tcgccggctg ggtgctgcgc agcgaagtgc
      121 aggcggtgat cgccgcgctc gaccgcaacg ccgccggcgc gctgctcgtc gtcgccgccg
      181 tgatcggcct ctggctgggc tggaagctgt ggcagaaata ccgcttccgc cgcctgtcgg
      241 cagtaccgca catcagcatc ggcgaactgc tcgccgcgct cgactcaaac cagccgccgc
      301 ttgtgctcga cctgcgcggc gccatcatgg ttgccgaaac cggccccatc cccggcgccg
      361 tcgtcgccga gcacgaccgc ctgttcgaag cggtgggcga ctggccacgg caccagccca
      421 tcgtcaccct ctgccactgc ccggaagacg ccggcgccat tcaggccgcc aaccgtctgc
      481 tcgatgccgg ctaccgctcg gtgcggccgc tgctcggtgg ttacgatgcg tggcgggcgg
      541 gcaaggcttg ctgagtcaga accgctgttg aactgacgtt gccgccgttt gaggcgtttg
      601 atcttcccgg cggaggtttg ccgatgccct ggctccgagc gaacgcgttt tgcccgacgg
      661 cgaccagccg ctgccggccg ggctcgggct gcgctggagc aggatgttga tggccagcgt
      721 cgcggtcagc agggcggcgg cgcccagggt atggaggacg gcgacgggca ggggcagcga
      781 gagcaggaca ttgcccaggc cgaggccgat ctgagctacc agcaggccga gcagcaggcc
      841 gccccagcgc cgccagcggc gatggccggt gcggatcagg gaaatggcca gcgcgccgac
      901 gatcagcgtc accgccagcg cgcccaggcg atggctccag tggatggcgg tcaggctggc
      961 cagcggcagc aactggccgt ccgcggtctg gccgagttcg cggtgcaggc tgaaggcatg
     1021 gtcaaactcc atctccggcc gccattgccc ctggcaggtc gggaaatcgg cgcaggcgag
     1081 ggccgcatag ttgctgctga cccagccgcc cagcgcgatc tgcagcacga tgagcagcaa
     1141 ggcgccgccc gcgagtagcc gcaaggcggg gctggcgggc gctgcgccgc ttgtttcgcg
     1201 ccgggcaaag agcatgccgg cgagcagcga gagcgtggcc atgccgccga gcagatgcag
     1261 cgtgacgatg accggcttga gcagataggt cacggtccac atgccgagcg ccgcctgcag
     1321 gcagacgatc agcagcaagg cggtgggcag cgccggcgac tgctgttggc ggcattcccg
     1381 gcgccaggcg aggacgcaca gcgtcagcac gagcaggccc aggctgccgg ccaggtagcg
     1441 gtgcagcatc tctttccagg ccttgccggt ttccagcggt cgttccgggt aggcctgcgc
     1501 cgccgccagt tgctcggccg gcgcgtcggg gacgccc
//
LOCUS       DAOSDX010000799         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27100_length_1535_cov_3.552027, whole genome shotgun sequence.
ACCESSION   DAOSDX010000799 DAOSDX010000000
VERSION     DAOSDX010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27100_length_1535_cov_3.552027"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLN02_14420"
     CDS             complement(<1..507)
                     /locus_tag="PLN02_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOI53571.1"
                     /translation="MASTDIRLTVAEERTIYDVAAIDRALEDAAGNRNEALAALYEKM
                     KQRGGGRFLIRPSGTDMLDDLYDSCPNFGEVVDDLRKYLALSVAGNEPMQFTPILLLG
                     EPGIGKTHFARELAARLGTGHEFVSMSSLTAGWVLSGASAQWNNARPGKVAHTLVHGD
                     YANPIVVLD"
     gene            660..1124
                     /gene="nikR"
                     /locus_tag="PLN02_14425"
     CDS             660..1124
                     /gene="nikR"
                     /locus_tag="PLN02_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOI53572.1"
                     /translation="MERFTISLDESLARQFDELIAARGYVNRSEAVRDLIRGAIEHDR
                     QQGEPTGQCVANLSYVYNHHERELSERLTGLQHDHHDLTVAAMHAHLDHDHCLESVIL
                     RGSTAAVRQFADAMIAERGVRHGKLNIVALEAEYHHAHDAQGSDHVHYHPTR"
     gene            1099..>1535
                     /locus_tag="PLN02_14430"
     CDS             1099..>1535
                     /locus_tag="PLN02_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOI53573.1"
                     /translation="MSTTIPPADPLLAATGEQAWIEVIQKMDEVYNDLLQYEVALEEK
                     NAALEESHQFIESVLAAMSDILIVCDRNGGIVEVNPALCHLSGRSSEELVSTPVFNLF
                     ADETEQAKARDAFARYARDGLHDCEFFLRASDGSTVPVSMNCTP"
BASE COUNT          275 a          523 c          484 g          253 t
ORIGIN      
        1 gtcgagcacc acgatggggt tggcgtagtc gccatgcacc aaggtgtgcg ccaccttgcc
       61 cgggcgggcg ttgttccact gcgccgaagc accggacagc acccagccgg cggtgagcga
      121 actcatcgac acgaattcgt ggccggtgcc gagacgggca gccagttcgc gcgcaaagtg
      181 ggtcttgccg atacccggtt cgccgagcag caggatcggc gtgaactgca tcggctcgtt
      241 gccggcgacc gacagcgcca ggtacttgcg caggtcgtcg acgacttcgc cgaagttcgg
      301 gcaactgtcg tagaggtcgt cgagcatgtc ggtgccgctc ggccggatca ggaagcggcc
      361 gccgccgcgc tgcttcatct tctcgtagag ggcggccagc gcttcgttgc ggttgccggc
      421 ggcgtcctcg agggcgcggt cgatcgccgc gacgtcgtag atggtgcgct cttcggcgac
      481 ggtgagacgg atgtcggtgc ttgccatgat gacttcctcc tgacgcgact gcagttgact
      541 gccttaacgg gtggacgatg gccggggttg aatccggcca ccccggtttc aacaagcatt
      601 tcccgttcca gccggtttgg gaaataatgc gttgcatctc ccccgaccgc aggaacgcca
      661 tggaacgctt caccatttcg ctcgatgaat cgctggcccg ccagttcgac gaactgatcg
      721 ccgcccgcgg ctacgtcaac cgctcggaag cggtgcgcga cctgatccgc ggcgccatcg
      781 agcacgaccg ccagcagggc gagccgactg gccagtgcgt cgccaacctg tcctacgtct
      841 ataaccatca cgaacgcgaa ctgagcgagc gcctgaccgg cctgcagcac gaccaccacg
      901 acctgacggt ggcggcgatg cacgctcacc tcgaccacga tcattgtctg gaatccgtca
      961 tcctcagggg ctcgaccgcg gctgtccgac aattcgccga cgccatgatc gccgagcgcg
     1021 gcgtgcgcca cggcaagctc aacatcgtcg ccctcgaggc cgaataccac cacgcccacg
     1081 acgcgcaggg cagcgaccat gtccactacc atcccacccg ctgacccgct gctcgccgcg
     1141 accggcgagc aggcctggat cgaggtgatc cagaagatgg acgaggtgta caacgacctc
     1201 ctgcagtacg aagtggcgct cgaagaaaag aacgccgccc tcgaggagtc gcaccagttc
     1261 atcgaaagcg tgctggcggc gatgtcggac atcctcatcg tctgcgaccg caacggcggc
     1321 atcgtcgaag tcaacccggc gctctgccac ctttccggcc gcagttccga ggagctggtc
     1381 agcacgccgg tgttcaacct cttcgccgac gagacggaac aggccaaggc gcgcgatgcc
     1441 ttcgcccgct acgcgcgcga cggcctgcac gactgcgagt tcttcctgcg cgccagcgac
     1501 ggttcgacgg tgccggtgtc gatgaactgc acgcc
//
LOCUS       DAOSDX010000800         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27128_length_1534_cov_2.826234, whole genome shotgun sequence.
ACCESSION   DAOSDX010000800 DAOSDX010000000
VERSION     DAOSDX010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27128_length_1534_cov_2.826234"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1455
                     /locus_tag="PLN02_14435"
     CDS             7..1455
                     /locus_tag="PLN02_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOI53574.1"
                     /translation="MAVEPLRYEKLAGELAGMIGDGILKHGDRLPSVRRLSQERRLSV
                     STVVQAMRQLEERGLVEARPQSGYFVRPPAMARREPEVRATPDGPMPVDISQRLVHVL
                     QAGAKPGVAPLSAALPANELLPIAALHRLYAGVARRHPHLLETGSHVHMDLPPLVRQL
                     VRRSLAWGGPLAAEEFVITNSCTEALNLCLRAVTRPGDTVAVESPAYYLMLQLLETLG
                     LKALEIPCDPRTGMSVEALELATRNGGVAACLLVANGSNPLGSVMPDERKRRLAALMA
                     ARGIPVIEDDIYGDLHFGDERPWPVKAFDDSGNVMLCASFSKCLSPSLRIGFIAAGRH
                     KPAVALQKTITSGGTNPLTQMVLAEYLESGAVDRHLHSLRRTYRRQVESMQAAVSRYF
                     PASTRIAQPQAGYVLWVELPEGIDTTQLHGRALRERLSYVPGELFSASGMYRNCLRLN
                     CGNPHTPEIDDAIRRLGRIFGGGESAAKEGRA"
BASE COUNT          233 a          542 c          511 g          248 t
ORIGIN      
        1 tgtgccatgg ccgtagaacc ccttcgttac gagaaactcg ccggcgagct ggccggcatg
       61 atcggtgacg gcatcctcaa gcacggcgac cgcctgcctt cggtgcgccg cctgtcgcag
      121 gagcgccggt tgtcggtgtc caccgtggtg caggccatgc gccagctcga ggagcgtggg
      181 ctggtcgagg cccggccgca atccgggtat ttcgtccggc cgccggcgat ggcgcggcgc
      241 gagccggaag tgcgggcgac gccggacggg ccgatgccgg tcgatatctc gcagcgtctg
      301 gtgcatgtcc tgcaggccgg cgccaagccc ggcgtggcgc cgctctccgc cgcgctgccg
      361 gccaacgagc tgctgccgat cgccgccctg catcgccttt acgctggcgt cgcgcggcgc
      421 catccgcacc tgctcgaaac cggcagccac gtccatatgg acctgccgcc gctggtccgc
      481 cagctggtgc gccgctcgct ggcctggggt gggccgctgg cggccgagga attcgtcatc
      541 accaactcct gcaccgaggc gctcaacctc tgcctgcgcg cggtgacccg gccgggcgac
      601 acggtcgccg tcgaatcgcc ggcttattac ctgatgctgc aactgctcga gacgctcggt
      661 ctgaaggcgc tggaaatccc ctgcgacccg cgcaccggga tgtcggtcga ggcgctcgaa
      721 ctggccaccc gcaacggcgg cgtcgccgcc tgcctgctcg tcgccaacgg cagcaacccg
      781 ctgggcagcg tcatgcccga cgagcgcaag cggcgcctgg cggcgctcat ggcggcgcgc
      841 ggcatcccgg tgatcgagga cgacatctac ggcgacctgc atttcggcga tgaacggccg
      901 tggccggtca aggccttcga cgacagcggc aacgtcatgc tctgcgcgtc gttctccaag
      961 tgcctgtcgc cgtccttgcg catcggcttc atcgccgccg gccgccacaa gccggcagtc
     1021 gcgttgcaga agacaatcac cagcggcggc accaacccgc tgacgcagat ggtgcttgcc
     1081 gaatacctcg aatcgggcgc cgtcgaccgg catctgcaca gcctgcgccg gacctaccga
     1141 cgccaggtgg agagcatgca ggcggcggta tcgcgctatt ttccggcaag cacccggatc
     1201 gcccagccgc aggccggcta cgtgctgtgg gtcgaactgc cggaaggcat cgataccacc
     1261 cagttgcacg ggcgcgcctt gcgcgagcgc ctgtcctacg tgccgggcga actgttttcg
     1321 gccagcggca tgtaccgcaa ctgcctgcgc ctcaattgcg gcaacccgca cacgccggaa
     1381 atcgacgatg ccatccgtcg cctcgggcgc attttcggag gcggcgaaag cgctgcgaag
     1441 gagggcaggg cttgagcaac tacggtatca ttgccccttt tgcggcggct gacgtgccgc
     1501 tgcagtgaac gtactcgaag gattttctgc tcat
//
LOCUS       DAOSDX010000801         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27145_length_1533_cov_3.434371, whole genome shotgun sequence.
ACCESSION   DAOSDX010000801 DAOSDX010000000
VERSION     DAOSDX010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27145_length_1533_cov_3.434371"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLN02_14440"
     CDS             <1..587
                     /locus_tag="PLN02_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI53575.1"
                     /translation="QSNLRRLLAFAVVSHTSLLVIGLFSLHGAGIQGAVLLAANFGLA
                     VTGMWFIVGFVFRRTGTTELGELSGLFERIPFLAVAFLTCGLAIVGMPGTPGFDAAHL
                     VLEAAIDRFGALSTVATALGNVAAAGFLLWAFQKAFLSQEKTAGRDVDRTLAMEYAVC
                     GIALAALLAIGFFTEPWLRLTDTASQALAARFVQ"
     gene            584..>1533
                     /locus_tag="PLN02_14445"
     CDS             584..>1533
                     /locus_tag="PLN02_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOI53576.1"
                     /translation="MSPLFPELPVLSLLVLLPVAAAGLLWLLPARLARPATFVALAAV
                     LALATLALFSYRPETAGFQLMERWPWLTGLGVDWLLAVDGLSVLFLPASALLFLGALT
                     VAGDAGANAPRLFHSLLLLLLAATLGIFCAIDLVVFFVFWEFSLVPVYFLLARWGATA
                     GAPQVAARYVLIMLAGGFPLLLAFVVVGSAGGSFDLTVLLGHPLPLREQRIVLVLMLI
                     GFGLKLPLVPLHTWLPQFALAAPASLSALLLGLKVGAYGLVRFAIPLAPQAAQDWHWL
                     LAGFGTVTLIYGAVGVLAHSNLRVGMAYASICHVGLATLG"
BASE COUNT          146 a          537 c          526 g          324 t
ORIGIN      
        1 tgcagagcaa cctgcgccgg ctgctcgcct tcgccgtggt cagccatacc agcttgctgg
       61 tgatcggcct gttcagcctg cacggcgccg gcatccaggg ggcggtgctg ctcgccgcca
      121 acttcgggct ggcggtgacc ggcatgtggt tcatcgtcgg tttcgtcttc cggcgcaccg
      181 ggaccaccga gttgggcgaa ttgtccggtc tgttcgagcg cattcccttt cttgccgtcg
      241 ccttcctgac ctgtggtctg gccatcgtcg gcatgcccgg cacgcccggc ttcgacgccg
      301 cccacctggt gctggaggcg gcgatcgacc gcttcggcgc gttgtccacg gtcgccaccg
      361 cgctcggcaa cgtggccgcc gccggcttcc tgctctgggc cttccagaag gccttcctgt
      421 cgcaggagaa gacggcgggg cgcgacgtgg accggacgct ggcgatggaa tacgcggtct
      481 gcggcatcgc cctggcggcg ttgctggcga tcggtttctt caccgaaccc tggctgcgcc
      541 tgaccgatac cgccagccag gcgctggccg cgaggttcgt ccaatgagcc cgctgtttcc
      601 tgaactaccc gtgctctcgc tgctggtgtt gctgccggtt gccgccgccg ggctgctctg
      661 gctgttgccg gcccgcctgg cgcggccggc gactttcgtc gcgctggcgg cggtgctggc
      721 gctggcgacg ctggcgctgt tttcctatcg tccggagacg gccggtttcc agctcatgga
      781 acgctggccg tggctgaccg ggctcggtgt cgactggctg ctcgcggtgg atgggctgtc
      841 ggtgctattc ctgccggcga gcgccttgct cttcctcggc gcgctgactg tcgccggcga
      901 cgccggcgcc aacgcgccgc gcctcttcca cagcctgctc ctgcttctgc tggcggcgac
      961 gctgggcatc ttctgtgcga tcgacctggt cgtcttcttc gtcttctggg aattcagcct
     1021 ggttccggtc tatttcctgc tggcgcgctg gggcgccacc gccggggcgc cgcaggtcgc
     1081 cgcgcgctac gtgctgatca tgctcgccgg cggctttccg ctgctgctcg cctttgtcgt
     1141 cgtcggcagc gccggcggca gtttcgacct gacggtcttg cttggccatc cgctgccgct
     1201 gcgcgagcag cgcatcgtcc tcgtcctgat gctgatcggc ttcggcttaa agctgccgct
     1261 ggtgcccttg catacctggc tgccgcagtt cgcgctggcg gcgccggcca gcctgagcgc
     1321 gctgctgctc gggctgaagg tcggcgccta cgggctggtc agattcgcca ttccgctggc
     1381 gccgcaggcg gcgcaggact ggcactggct gctcgccggt ttcggcacgg tgacgctgat
     1441 ctacggcgcg gtcggcgtcc tggcgcacag caacctgcgc gtcggcatgg cctacgccag
     1501 catctgccac gtcgggctgg cgacgctcgg ttt
//
LOCUS       DAOSDX010000802         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27176_length_1532_cov_2.272173, whole genome shotgun sequence.
ACCESSION   DAOSDX010000802 DAOSDX010000000
VERSION     DAOSDX010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27176_length_1532_cov_2.272173"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..>1532)
                     /locus_tag="PLN02_14450"
     CDS             complement(112..>1532)
                     /locus_tag="PLN02_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOI53577.1"
                     /translation="RYLKSLQIKDLALDHSMISLGSCTMKLNATSEMIPVTWPEFGGI
                     HPFAPREQVAGYHEMIAGLTEWLKTVTGFDAICMQPNSGAQGEYAGLVAIARYHASRG
                     DSHRKICLIPKSAHGTNPATAQMAGMQVVVVDCDDNGNVDVADLKAKAEEHKDDLACL
                     MITYPSTHGVFEEAIRDICAIVHANGGQVYMDGANLNAQVGLTSPGFIGADVSHMNLH
                     KTFAIPHGGGGPGMGPIGLKAHLAPFMANHVVAPIDGPHGGQGAVSAAPFGSASILPI
                     SWMYLAMLGGAGVKKATQFAILNANYVASRLKDHYPVLYVGKNGRVAHECILDIRPLK
                     AATGIAEIDIAKRLMDYGFHAPTVSFPVAGTIMVEPTESESKAELDRFIEAMVAIRAE
                     IREIENGTWSATDNPLKNAPHTQADLVDAEWRHPYSRQQAVFPLPWVAANKFWPSVNR
                     IDDVYGDRNLNCACPPLSAYQD"
BASE COUNT          234 a          438 c          555 g          305 t
ORIGIN      
        1 gttcgccgca gtatcagaag gatggtcagg ccttgcgctg acacgaaatt attccttcgc
       61 aattgaaaac ggcctcttgc gaggccgttt ttgtttttac cgagagcgtt ttcagtcctg
      121 gtaagccgac agcggcgggc aggcgcagtt caggttgcgg tcaccgtaca catcgtcgat
      181 gcgattgacg cttggccaga acttgttggc cgctacccag ggcagcggga agactgcctg
      241 ttgccggctg tagggatggc gccactcggc atcgacgagg tcggcctggg tgtgcggcgc
      301 gttcttcagc gggttgtcgg tggcgctcca ggtgccgttc tcgatctcgc ggatttccgc
      361 gcggatcgcc accatggctt cgatgaaacg atccagttcg gccttcgatt ccgactcggt
      421 cggctcgacc atgatggtgc cggcgaccgg gaaggatacc gtcggggcgt ggaagccgta
      481 gtccatcagg cgcttggcga tgtcgatctc ggcgatgccg gtggcggctt tgagcgggcg
      541 gatgtcgagg atgcactcgt gcgccacgcg gccgttcttg ccgacgtaga gcacggggta
      601 gtggtccttg agccggctgg cgacgtagtt ggcgttgagg atggcgaact gggtcgcctt
      661 cttgacgccg gcaccgccga gcatggccag gtacatccag gaaatcggca ggatcgaggc
      721 ggagccgaag ggcgccgcgg aaaccgcgcc ctggccgccg tgcgggccgt cgatcggggc
      781 aaccacgtgg ttggccatga acggagcgag gtgggccttg aggccaatcg ggcccatgcc
      841 cggtccgccg ccgccgtgcg ggatggcgaa ggtcttgtgc aggttcatgt ggctgacgtc
      901 ggcgccgatg aagccgggcg aggtcaggcc gacctgggcg ttgaggttgg cgccgtccat
      961 gtacacctgg ccgccgttgg cgtgcacgat ggcgcagatg tcgcggatgg cttcctcgaa
     1021 gacgccgtgc gtcgacgggt aggtgatcat caggcaggcg aggtcgtcct tgtgttcctc
     1081 ggccttggcc ttcaggtcgg cgacatcgac gttaccgttg tcgtcgcaat cgacgacgac
     1141 gacctgcatg ccggccatct gggcggtggc cgggttggta ccgtgcgccg atttcggaat
     1201 caggcaaatc ttgcggtggc tgtcgccgcg gctggcgtgg tagcgggcaa tcgccaccag
     1261 gccggcatat tcgccctggg cgccggagtt cggctgcatg cagatggcgt cgaagccggt
     1321 caccgtcttc agccattcgg tgaggccggc gatcatttcg tggtagccgg cgacctgctc
     1381 gcgcggggcg aacgggtgga tgccgccgaa ttccggccag gtgaccggga tcatttcgct
     1441 ggtcgcgttc agcttcatgg tgcaggagcc gagcgagatc atcgagtggt cgagcgccag
     1501 gtccttgatc tgcagcgact tcaggtagcg ca
//
LOCUS       DAOSDX010000803         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27190_length_1531_cov_2.842818, whole genome shotgun sequence.
ACCESSION   DAOSDX010000803 DAOSDX010000000
VERSION     DAOSDX010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27190_length_1531_cov_2.842818"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..995
                     /gene="rfbD"
                     /locus_tag="PLN02_14455"
     CDS             99..995
                     /gene="rfbD"
                     /locus_tag="PLN02_14455"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOI53578.1"
                     /translation="MGILLLGKDGQLGWELQRALAPLAPLVACGRGECDLSDPAALRV
                     LVAAARPAVIVNAAAYTAVDKAESDAATAHAINAEAPRVLAECAAASGGLLIHYSTDY
                     VYDGNKASPYVETDAVAPQSVYGRSKLSGEEGIRASGARSLVFRTSWVFGEHGGNFVR
                     TVLRLARERDSLSVVDDQVGSPTPAALIADVTAAALLRLDLGRRLGDGESRLYHLAAA
                     NPLSWCGFARRIVELAGGRAGGLRFDADAVRAIPSAEYPTPARRPANSRLDCSRLAVD
                     FDLHLPDWEPYLQRMLAQIVAR"
     gene            1202..>1531
                     /gene="rpsB"
                     /locus_tag="PLN02_14460"
     CDS             1202..>1531
                     /gene="rpsB"
                     /locus_tag="PLN02_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOI53579.1"
                     /translation="MSVTMREMLEAGVHFGHQTRFWCPKMAPYIFGARNKIHIVNLEK
                     TLAKYNEAMAFVKKLSANRGNILFVGTKRQAREIVAEEAARAGAPCVDQRWLGGMLTN
                     FKTVKTSI"
BASE COUNT          258 a          454 c          509 g          310 t
ORIGIN      
        1 ctggggtaaa ggagcaagga taaatgatgc cccccgtttc gcaaagctgg ccggccgaaa
       61 atggcatact gcgcgggttt tgacggagga agcggggcat gggaatcttg ttgctgggca
      121 aggatggtca gttgggttgg gaattgcaac gcgcgctggc accactggcg ccgctggtcg
      181 cttgcgggcg tggcgagtgc gacctctccg atcccgccgc gttgcgggta ttggttgctg
      241 ctgcccggcc ggcggtgatc gtcaatgcgg cggcgtacac ggccgtggac aaggcggaat
      301 cggatgccgc cacagcgcat gccatcaatg ccgaggcgcc gcgcgtcctc gctgaatgtg
      361 ctgcggcaag cggtgggctg ctgattcact attcgaccga ttacgtgtac gacggcaaca
      421 aggcgtcgcc ctacgtcgaa accgacgctg tggcgccgca gagcgtctat ggccgcagca
      481 agctgtccgg tgaggaaggg atacgcgcca gcggcgcccg ttcattggtt ttccgcacga
      541 gttgggtctt cggtgagcat ggcggcaatt ttgtccgcac cgtcctgcgc ctggcgcgcg
      601 agcgcgacag cctgtcggtg gttgatgacc aggtcggttc gccgacgccg gcggcgctga
      661 tcgccgacgt caccgcagcg gccctgctgc gcctggatct cggccgccgg ctgggcgacg
      721 gggaaagccg cctctatcac ctggcagcgg cgaatccgct gagctggtgc ggctttgccc
      781 ggcgcatcgt cgagttggcg ggcggtcgtg ccggcggcct gcgtttcgat gccgacgccg
      841 tccgggcgat ccccagcgcc gaatatccaa cgccggcgcg gcgtccggcc aactcccgtc
      901 tcgattgcag tcgcttggcc gtcgatttcg atttgcatct gccggactgg gagccgtatc
      961 tgcaacggat gctggcgcag atcgtcgcca gatgacaaat gccgtctcgc ctggcttttg
     1021 gtcgggcgaa ttcgctgcta taatcctgcg gtttttccgg gtctgtgact cgggaatgac
     1081 ggcgatgcgc gtggtgcgtg tcgtcaaatc ctcacatccg ccgattaagg gtgttcgtca
     1141 gattccaata atgacgggcg tttgcggcgg gtggcggttc aacccttaaa ggagttttgc
     1201 aatgtccgtt accatgcgtg aaatgctgga agccggtgtc cacttcggcc accagacccg
     1261 tttctggtgc cccaagatgg ccccgtacat tttcggtgcc cgcaacaaga ttcatatcgt
     1321 caacctggaa aagaccctgg ccaagtacaa cgaagccatg gctttcgtga agaagctgtc
     1381 ggccaatcgc ggcaacatcc tgtttgtcgg taccaagcgt caggcccgcg aaatcgtcgc
     1441 tgaagaagcg gcgcgcgccg gtgctccttg tgttgaccag cgctggctgg gcggcatgct
     1501 gaccaacttc aagacggtca agacctcgat c
//
LOCUS       DAOSDX010000804         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27278_length_1527_cov_2.982337, whole genome shotgun sequence.
ACCESSION   DAOSDX010000804 DAOSDX010000000
VERSION     DAOSDX010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27278_length_1527_cov_2.982337"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLN02_14465"
     CDS             complement(<1..208)
                     /locus_tag="PLN02_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein"
                     /protein_id="HOI53580.1"
                     /translation="MKKLLSISLLLALIAGCSTTPAPDENAGAPVESRSASQQPDVTP
                     VVADGLDAGGLPRVLTDPKSQLSQR"
     gene            complement(228..1499)
                     /gene="tolB"
                     /locus_tag="PLN02_14470"
     CDS             complement(228..1499)
                     /gene="tolB"
                     /locus_tag="PLN02_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOI53581.1"
                     /translation="MSRISRRIFLIFSLLGAGLAQAQLSIEITGAGANRIPVTIAEFA
                     GDASLSRDITEIVRADLERSGLFRMVPPNAGTMDENAPVDYAAWKARGSDALAAGSVL
                     RAADGRIEARFRLYDTQKGEPLGGAVYVTGKAQMRMAGHRIADFIYEKLTGERGVFST
                     RIAYVVKSRGQYLLQVADADGQGAATALTSAEPIISPVWSPDGSRLAYVSFEKKKPMI
                     YVHSLASGQRRIVANFKGSNSAPAWSPDGRKLAVVLSKDGNSQLYSVNADGSGLQRLS
                     QSSGIDTEPRFSADGSSIYFTSDRGGSPQIYRVDANGGSAQRVTFEGSYNVSPRPSPD
                     GRTLAFISRREGRFQLAVMDLASRQVQVLTDSTKDESPSFAPNGRTILIATETGGRGV
                     LSAVSADGRIKQRLTIAAGDVREPAWGPFLK"
BASE COUNT          241 a          454 c          567 g          265 t
ORIGIN      
        1 tgcgttgcga gagctggctc ttcggatcgg tcaggacgcg cggcaggccg ccggcgtcga
       61 ggccatcggc gaccaccggg gtgacgtcgg gctgctggct ggcgctgcgg ctttcgaccg
      121 gggcgccggc attttcatca ggggcggggg tggtgctgca gccggcgatc agggcgagca
      181 gcagggaaat cgacaacagt tttttcatga ttttctccga cgaggttcta tttcaggaat
      241 gggccccatg ccggctcgcg gacgtcgcca gcggcaatgg tgaggcgttg ttttatacga
      301 ccgtcggcgg agacggcgga gaggacgccg cgcccgccag tttccgtggc gatcaggatc
      361 gtccggccgt tcggggcaaa gctcggcgat tcgtccttgg tcgaatcggt gagcacttgt
      421 acctggcggc tggcgaggtc catgaccgcc agctggaaac gtccctcgcg ccgggagatg
      481 aaggccagcg tgcggccgtc cggcgaggga cggggcgaaa cgttgtagct gccttcgaaa
      541 gtgacgcgct gggcggagcc cccgttggcg tcaacccggt agatctgcgg actgccgccg
      601 cgatccgagg tgaaatagat gctcgatccg tccgccgaga agcgcggttc ggtgtcgatg
      661 ccggaagact ggctcaggcg ctgcaggccg ctgccgtcgg cgttgaccga gtacaactgg
      721 gaattgccgt ccttggagag gacaaccgcc agcttgcgac cgtccggcga ccaggccggg
      781 gccgagttgg agcccttgaa gttggcgacg atgcggcgct ggccggacgc cagcgaatgc
      841 acgtagatca tcggcttctt cttttcgaag gagacatagg cgaggcggct gccgtccggc
      901 gaccagaccg gcgagatgat cggttcggcg gaagtcagcg cggtggctgc gccctggccg
      961 tcggcgtcgg cgacctgcag caggtactgg ccgcgcgatt tgacgacgta ggcgatgcgc
     1021 gtcgagaaga cgccgcgttc gccggtcagc ttctcgtaga tgaaatcggc gatgcggtgg
     1081 ccggccatgc gcatctgcgc cttgccggtg acatagacgg cgccgcccag cggctcgccc
     1141 ttctgcgtgt cgtagagacg gaaacgcgcc tcgatgcgac cgtcggccgc gcgcagcacg
     1201 ctgccggcgg ccagcgcgtc gctgccgcgc gctttccagg cggcgtaatc gaccggcgca
     1261 ttctcatcca tcgtgccggc gttcggcggc accatgcgga acaggccgct gcgttcgagg
     1321 tcggcgcgga cgatctcggt gatgtcgcgc gacaggctgg cgtcgccggc aaattcggcg
     1381 atggtgacgg gaatccggtt ggcgccggcg ccggtgatct cgatcgagag ctgggcctgg
     1441 gccaggccag cgccgagcag gctgaaaatc aggaagatac ggcgggaaat tctggacatt
     1501 ggtggcggtt tctctgaatt gtgcgcg
//
LOCUS       DAOSDX010000805         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27309_length_1526_cov_2.598912, whole genome shotgun sequence.
ACCESSION   DAOSDX010000805 DAOSDX010000000
VERSION     DAOSDX010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27309_length_1526_cov_2.598912"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /gene="thiC"
                     /locus_tag="PLN02_14475"
     CDS             complement(<1..1314)
                     /gene="thiC"
                     /locus_tag="PLN02_14475"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOI53582.1"
                     /translation="MNAKDPFLAANAHVDEAAIQPLPNSRKVYIEGSRPDLRVPMREI
                     SQADTPTGFGGEKNPPIYVYDCSGPYSDPAAQIDIRSGLPALRAQWIIERGDTEELPD
                     LSSEFGRQRAADKALDELRFPGLHRKPRRAKAGANVSQMHYARKGIITPEMEYVAIRE
                     NLNRAAYIESLKATGPTGEKMAKLLTRQHPGQSFGASIPAEITPEFVRSEVARGRAII
                     PNNINHPESEPMIIGRNFLTKINANIGNSALGSSIQEEVEKMTWSIRWGGDTVMDLST
                     GKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKAEDLTWEIFRDTLIEQAEQGVDYF
                     TIHAGVLLRYIPMTANRMTGIVSRGGSIMAKWCLAHHKESFLYTHFEDICEIMKAYDV
                     AFSLGDGLRPGSIYDANDEAQLGELKTHGELTEIAWKHDVQTIIEG"
     regulatory      complement(1369..1468)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          242 a          426 c          528 g          330 t
ORIGIN      
        1 cccttcgatg atggtctgca cgtcgtgctt ccaggcgatt tcggtgagtt cgccgtgggt
       61 cttcagctcg cccagttgcg cttcgtcgtt ggcgtcgtag atcgagccgg ggcgcaggcc
      121 gtcgccgagg ctgaaggcga cgtcgtaggc cttcatgatc tcgcagatgt cctcgaagtg
      181 cgtgtagagg aagctttcct tgtggtgcgc caggcaccac ttggccatga tcgagccgcc
      241 gcggctgacg atgccggtca tgcggttggc ggtcatcggg atgtagcgca gcagcacgcc
      301 ggcgtggatg gtgaagtaat cgacgccctg ttcggcctgt tcaatcagcg tgtcgcggaa
      361 gatttcccag gtcaggtcct cggccttgcc gttgaccttc tccagcgcct ggtagatcgg
      421 cacggtaccg atcggcaccg gcgagttgcg gatgatccac tcgcgcgttt cgtgaatgtt
      481 cttgccggtg gacagatcca tcaccgtgtc gccgccccag cggatcgacc aggtcatttt
      541 ctcgacttct tcctgaatgc tggagccaag cgccgagttg ccgatgttgg cgttgatctt
      601 ggtcaggaag ttgcgaccga tgatcatcgg ctcgctttcc gggtggttga tgttgttcgg
      661 gatgatggcg cggccgcggg cgacctcgct gcgcacgaat tccggcgtga tttcggccgg
      721 aatcgaagcg ccgaagctct ggcccggatg ctggcgggtc agaagtttgg ccatcttctc
      781 gccggtcggg ccggtggctt tcagcgattc gatataggcg gcgcgattga ggttctcgcg
      841 gatcgccacg tactccattt ccggggtgat gatgcctttg cgcgcgtagt gcatctgtga
      901 cacattggcg ccggccttgg cccggcgtgg tttgcggtgc aagccgggga agcgcagttc
      961 gtcgagagcc ttgtcggcgg cacgctggcg gccgaattcg gaagacaggt cgggcagttc
     1021 ctcggtgtcg ccgcgctcga tgatccattg ggcgcgcagc gccggcagac cggagcggat
     1081 gtcgatctgc gccgccgggt cggaatacgg gccggaacaa tcataaacgt agatcggcgg
     1141 attcttctcg ccgccgaagc cggtcggcgt atcggcctgc gagatttcgc gcatcggcac
     1201 ccggaggtcc ggccgcgagc cttcgatgta aaccttgcgc gaattcggca gcggctggat
     1261 ggcggcctcg tccacgtggg cgttggcggc gaggaagggg tctttggcgt tcatgagtgc
     1321 tccgtgcgga tgttcagcgg ggtaagacgg gcaaggagcg atctggttcc ggtaacggat
     1381 tcgtttccct acgtcggcat gacccgatca ggttcgaagg gtatttctca gccgagcgct
     1441 gtgcgggtcg acacccctaa cgagagtggt gcaaggtact ggaaacacag gcaaaatgca
     1501 aggaagcacg gtcggggctc aagatt
//
LOCUS       DAOSDX010000806         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27314_length_1526_cov_2.200544, whole genome shotgun sequence.
ACCESSION   DAOSDX010000806 DAOSDX010000000
VERSION     DAOSDX010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27314_length_1526_cov_2.200544"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PLN02_14480"
     CDS             <1..1182
                     /locus_tag="PLN02_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53583.1"
                     /translation="DYDPAYKAQAAIAGERAEHELNARWGRAVAVDPLWNPNLSLTTT
                     SLLPETKYRAQWQFLPSDRPRIFACPIPNGQGDYRITSPLKAARKLGLASQCVWMQST
                     KNPNYLTVPELLRLAPDTAIVQNYIFDRPLAALDRWHRAPGRPFTVYALDDLITGLDP
                     SNPFFKNIPANARARLQYALDRCDRMVVSTDFLADRYSSFIRDIRVAPNCLEQEIWLP
                     LRSQKRTGKRPRIGWAGGSTRHADLLLLREIIERTRHEADWVFFGMCPREVLPLLAEF
                     HDTIAFEDYPAYLASLNFDIAVAPLAQTEFNRGKSNLRLLEYGILGIPVVCTDIDPYR
                     DSPACRVPNIADAWVEALRARIHDADAREREGVAMRDWVCQNYLLENRVNDWLAAHLP
                     D"
     gene            1341..>1526
                     /locus_tag="PLN02_14485"
     CDS             1341..>1526
                     /locus_tag="PLN02_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOI53584.1"
                     /translation="MSQINTNTYSLNAQKNLANNSRGLASALERLSSGLRVNSAKDDA
                     TGLASGMKIDASARLTAV"
BASE COUNT          303 a          558 c          413 g          252 t
ORIGIN      
        1 gattacgatc ccgcctacaa ggcacaggcg gcgatcgccg gcgaacgggc ggagcacgaa
       61 ctcaatgcac gctggggccg cgccgtcgcc gtcgacccct tgtggaaccc caacctgtcg
      121 ctgaccacga caagcctgct gccggaaacc aagtaccggg cgcaatggca gttcctgccc
      181 agcgaccggc cacgcatctt tgcctgcccg atccccaacg gccagggcga ctaccgcatc
      241 acctcgccgc tgaaagccgc ccgcaaactc ggcctggcga gccagtgcgt gtggatgcag
      301 agcaccaaga acccgaacta cctgaccgtc ccggagctgc ttcgtctcgc accggacacg
      361 gcgatcgtcc agaactacat cttcgaccgt ccgctcgccg cgctcgatcg ctggcaccgg
      421 gcgcccggcc gaccattcac cgtctacgcg ctggacgacc tgatcaccgg cctcgacccg
      481 agcaatccgt tcttcaagaa catcccggcc aacgcccgcg cccgcctgca atacgcgctc
      541 gaccgctgcg accggatggt cgtctccacc gatttcctgg ccgaccgcta cagcagcttc
      601 atccgcgaca tccgcgtcgc gcccaactgt ctcgaacagg aaatctggct gccgctgcgc
      661 agccagaagc gcaccggcaa acggccacgc atcggctggg ccggcggttc gacgcgccac
      721 gccgacctgc tgctgctccg cgaaatcatc gaacgcaccc gccacgaagc cgactgggtc
      781 ttcttcggca tgtgcccgcg cgaagtcctg ccgctgctcg ccgaattcca cgacaccatc
      841 gctttcgagg actacccggc ctatctcgcg tcgctgaatt tcgacatcgc cgtcgcgccg
      901 ctggcgcaga ccgagttcaa ccgcggcaag agcaacctgc gcctgctcga atacggcatt
      961 ctcggcatcc cggtggtgtg taccgacatc gacccctacc gcgacagccc ggcttgccgc
     1021 gttcccaaca tcgccgacgc ctgggtcgaa gccctgcgcg cccgcatcca cgatgccgat
     1081 gcgcgcgaac gcgaaggggt cgccatgcgc gactgggtct gtcagaatta cctgctggag
     1141 aatcgcgtga atgactggct ggccgcccat cttccggact gatttcccga catcgaaagt
     1201 ttttttgaaa atgcactaaa gaagttccga gccacgtcgt taatcaacct gtcagcggaa
     1261 cgatctccgc atcggcgggg tgttaaaggc caaatcgaaa cagggccgac ccagaagatc
     1321 aatttttcag gagaaagaaa atgtcgcaaa tcaacaccaa cacctactcg ctcaatgccc
     1381 agaagaacct ggccaacaac tcgcgtggtc tggcttccgc tctcgagcgt ctctcctccg
     1441 gtctgcgcgt caattccgcc aaggacgacg cgacgggtct ggcctccggc atgaagatcg
     1501 atgcctccgc ccgcctgacc gccgtt
//
LOCUS       DAOSDX010000807         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27327_length_1525_cov_3.528571, whole genome shotgun sequence.
ACCESSION   DAOSDX010000807 DAOSDX010000000
VERSION     DAOSDX010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27327_length_1525_cov_3.528571"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLN02_14490"
     CDS             complement(<1..489)
                     /locus_tag="PLN02_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOI53585.1"
                     /translation="MTTLICGSLAFDNIMVFPDRFKNHILPEQIHILNVAFLVPEMRR
                     EFGGCAGNIAYNLMLLGGDATIMATVGDDAAPYLARLDTLGLSRAHVRQVPGSFTAQA
                     FITTDLDDNQITAFHPGAMMRSYENHVKDVPDVRFGIVGPDGYEAMLQNAREFAECGI
                     PFI"
     gene            complement(493..1500)
                     /locus_tag="PLN02_14495"
     CDS             complement(493..1500)
                     /locus_tag="PLN02_14495"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HOI53586.1"
                     /translation="MPGRGPLLTRTRCPECGTVFRVTSEQLRLKAGKVRCGSCRGIFN
                     AFDQLLPLETPAVAALTPAVDGKPLPANPTPIGEAPADPAPPPEETFIEPGIPADTGP
                     DSVAMPILEEAQDPPWPEAEALEDEPEPEPAPPETPEQTTQAAREAGLVAVRELADSQ
                     AYNRWSAGPLADGGLSSFADDARPLTWPYWLAALLLLLTLGWQAAYHFRGDLVLRLPA
                     AGAVFSALGVPVPLPRLEHLVAIETSDLQSDQARGLFVLNATLKNRAAFAQAWPDLEL
                     TLTDSADQVVARRVIAARDFLSAGAPRNAFPANGETAVRLWIDAKGIGAAGYRLYIFY
                     P"
BASE COUNT          244 a          450 c          584 g          247 t
ORIGIN      
        1 gatgaaggga atgccgcatt cggcgaactc gcgcgcgttc tgcagcatcg cctcgtagcc
       61 gtccggcccg acgatgccga agcgcacgtc gggcacgtcc ttcacgtgat tctcgtagct
      121 gcgcatcatc gcgccggggt ggaaggcggt gatctggttg tcgtcgagat cggtggtgat
      181 gaaggcctgg gcggtgaagc tgccggggac ctggcggacg tgggcgcggc tcaggccgag
      241 tgtgtcgaga cgggccagat acggggcagc atcgtcgccg acggtggcca tgatcgtcgc
      301 gtcgccaccg agcagcatca ggttgtaggc gatgttgccg gcacagccgc cgaactcgcg
      361 gcgcatttcg ggaaccagga aggcgacgtt gaggatgtgg atctgctcgg gcaggatatg
      421 gttcttgaag cggtccggaa agaccatgat gttgtcgaag gccagcgagc cgcagatcag
      481 tgtcgtcatt tttcaggggt agaagatgta caggcgatag ccggcggcac cgatgccttt
      541 ggcatcgatc cacagccgga cggcggtttc gccgttggcc gggaaggcgt tgcggggggc
      601 gccggcggac aggaaatcgc gggcggcgat gacgcgccgg gcaaccacct ggtcggccga
      661 atcggtcagg gtcagttcga ggtcgggcca ggcctgggcg aaggcggcac ggttcttcag
      721 cgtcgcgttg aggacgaaca ggccgcgtgc ctggtcggat tgcaggtccg aggtttcgat
      781 ggcgaccaga tgctccaggc gcggcagcgg taccggcacg ccaagcgccg agaagacggc
      841 gccagcggcg ggcaggcgca gcaccagatc gccgcggaag tgataggcgg cctgccagcc
      901 gagggtcagc aacagcagca gcgcggccag ccagtacggc caggtcagcg gccgcgcatc
      961 gtcggcgaag ctggagagtc cgccgtcggc cagcgggccg gccgaccagc ggttgtaggc
     1021 ctggctgtcg gcgagctcgc ggaccgccac cagacctgcc tcgcgggcgg cctgggtagt
     1081 ctgttcgggg gtttccggtg gcgcgggctc aggttcgggt tcgtcttcca gtgcctcggc
     1141 ttccggccag ggcggatcct gcgcttcctc gaggatcggc atcgccacgc tgtcggggcc
     1201 ggtgtcggcg ggaatgcccg gttcaatgaa agtttcttcc ggcggtggtg ctggatcggc
     1261 tggcgcttcg ccgattggcg tcgggttggc cggcagcggc ttgccgtcga cagcgggcgt
     1321 aagcgcggcg acggcaggcg tttcgagcgg caacaactgg tcgaaggcgt tgaagatgcc
     1381 gcggcaactg ccacagcgga ccttgcccgc cttcagccgc aactgctcgg aggtgacacg
     1441 gaagaccgtg ccacattccg ggcagcgggt acgggtcagg agcggacccc ggccaggcat
     1501 atccagtcct cgcgggtgtc ggcca
//
LOCUS       DAOSDX010000808         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27329_length_1525_cov_3.026531, whole genome shotgun sequence.
ACCESSION   DAOSDX010000808 DAOSDX010000000
VERSION     DAOSDX010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27329_length_1525_cov_3.026531"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PLN02_14500"
     CDS             <1..905
                     /locus_tag="PLN02_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOI53587.1"
                     /translation="LNALPIFHSFGLTAGALLPVLTGANLFLYPSPLHYRVIPEVAYD
                     RSCTVLFGTSTFLGNYAKFAHPYDFYRLRYVVAGAEKLSEAVRSQWFEKFGVRIFEGY
                     GATETAPVLAVNTPMAYKTGTVGNLLPGIEHKLVPVPGIANGGILHVRGPNVMAGYLK
                     AEQPGVLQAPTSEAGDGWYETGDVVEMDDEGFVKIVGRVKRFAKIAGEMVSLEVVEKL
                     AVATAPDKPHAVSSQPDESKGEALVLFTTDPALSRDMLASKARELGLPELAVPRKILV
                     VEALPLLGTGKIDYVTLKQMAEAA"
     gene            902..>1525
                     /locus_tag="PLN02_14505"
     CDS             902..>1525
                     /locus_tag="PLN02_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53588.1"
                     /translation="MMKQLWRGRACALGALLSLAVGTLPAVEPDKLQRLGTWMAHYYE
                     HPEPEALAGWMREISASGSLEKTSARYPVMIFVSELARQNPDRVGGWCDGLAGLPTAH
                     RAAFAWSFRNAGAPGSERCLKDLPADAEAALRDLKGFSPLQRAPVTPGDLDLLWAVFV
                     ATGDERPVHMIVDVLSRPQPERGAAGSVEALLLNGAAKWSLASNARQH"
BASE COUNT          255 a          477 c          528 g          265 t
ORIGIN      
        1 tgctgaacgc gctgcccatc ttccacagct tcggcctcac tgccggcgcg ctgctgccgg
       61 tgctgaccgg cgccaacctc ttcctctacc cgtcgccgct gcattaccgg gtgattccgg
      121 aggttgccta cgaccgtagc tgcaccgtgc ttttcggtac ctcgaccttc cttggtaact
      181 acgccaagtt cgcccatccc tacgacttct accgcctgcg ttacgtcgtc gccggtgccg
      241 agaagctctc ggaagcagtg cgcagccagt ggttcgaaaa gttcggcgtg cgcatcttcg
      301 aaggttacgg cgcgaccgaa accgcgccgg tgctcgcggt caacacgccg atggcgtaca
      361 aaaccgggac cgtcggcaac ctgctgccgg gcatcgaaca caagctggtg ccggtgccgg
      421 gcatcgccaa cggcggcatc ctgcacgtgc gcggcccaaa cgtcatggcc ggctacctca
      481 aggccgaaca gcccggcgtg ctgcaggcgc cgacttcgga agccggcgac ggctggtacg
      541 aaaccggcga tgtcgtggaa atggatgacg aaggcttcgt gaagatcgtc ggccgggtca
      601 agcgcttcgc caagatcgcc ggcgagatgg tcagcctgga agtggtcgag aagctcgccg
      661 tcgccaccgc gccggacaag ccgcacgcgg tatcgagcca gccggacgaa agcaagggcg
      721 aggcgctggt gctgttcacc accgacccgg cgctgagccg cgacatgctg gccagcaagg
      781 cgcgcgaact tggcctgccg gaactggcgg tgccgaggaa gatcctggtg gtcgaggcgc
      841 tgccgctgct cggcactggc aagatcgatt acgtgacgct gaaacagatg gcggaggcgg
      901 catgatgaaa cagctttggc gaggacgggc ttgcgcgctg ggtgctctgc tgtcgctcgc
      961 cgtcggcaca ttaccggcgg tggagccgga taagctgcaa cggctcggta catggatggc
     1021 gcattactac gagcatcccg agccggaggc gttggcaggc tggatgcgcg aaatctcggc
     1081 gagcggcagt ctggaaaaga cgtcggcacg ctacccggtg atgatcttcg tgtcggagtt
     1141 ggcccgccag aatccagacc gggtcggtgg ttggtgcgat ggtttggcgg gcctgccgac
     1201 ggcgcaccgg gcagctttcg cgtggtcgtt caggaatgcc ggggcgcccg gaagcgagcg
     1261 ctgtctgaag gatttgccgg ccgatgccga ggccgcgctg cgcgatctca agggcttttc
     1321 accgctgcag cgggcgccgg tcacaccggg ggatctcgac ctgttgtggg cggttttcgt
     1381 ggccaccggt gacgaacggc cggtgcacat gatcgtcgat gtcctgtccc ggccgcagcc
     1441 cgagcgcggt gcggcgggta gcgtcgaggc tttgttgctg aacggcgcgg cgaagtggtc
     1501 gcttgcttcc aatgcccgcc agcac
//
LOCUS       DAOSDX010000809         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27398_length_1522_cov_3.675528, whole genome shotgun sequence.
ACCESSION   DAOSDX010000809 DAOSDX010000000
VERSION     DAOSDX010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27398_length_1522_cov_3.675528"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="flhF"
                     /locus_tag="PLN02_14510"
     CDS             complement(<1..495)
                     /gene="flhF"
                     /locus_tag="PLN02_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286506.1"
                     /note="positive regulator of class III flagellar genes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOI53589.1"
                     /translation="MNVRKFIAANARDALRKVKETLGNDAIILSNRGVPGGVEIMAVA
                     ARDMAMIVPTPAGDITPPPVERPAAPSVDDDYRVMLSSARARISGTMPPVQPQSPPAP
                     RPQAAAPQQRQAAQVNAGIPRTGALRQADVPPRPTTPPQRPQPQVAPAQTQAAPARRP
                     EAEVV"
     gene            complement(492..>1522)
                     /locus_tag="PLN02_14515"
     CDS             complement(492..>1522)
                     /locus_tag="PLN02_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOI53590.1"
                     /translation="SDVAPLDVLGLEIGYRLIPLVDKRQDGELLRRIRGIRKKFAQEV
                     GFLVSPVHIRDNLELKPNAYRILLKGVEVGAAEAYAGQFLAINPGRVAGTLSGTPTKD
                     PAFGLPAVWIDIMQRDQAQAYGYTVVDASTVVATHLNHLILTHAAELLGRQEVQQLLD
                     HLAKEMPKLVEDLVPKVLPLGSLQKILQGLLDEGVHIRDMRTIIETVADHALRTQSAD
                     DLIVQVRIALGRAIVQQLFPGNGEMQVMSLDPTLERLLAQAVAGGSENTSFEPGLADT
                     LVRETAAAAGRQEGLGLPAVLLVPSSLRLLLSRFLRRTVPQLKVLSHNEVPENRIIKV
                     TSIIGGRT"
BASE COUNT          230 a          488 c          531 g          273 t
ORIGIN      
        1 caccacttca gcctccgggc gccgggctgg cgcggcctgc gtctgcgctg gtgcgacttg
       61 cggctggggg cgttgcggcg gcgtggttgg gcgcggcggc acgtccgcct gacgtagggc
      121 gccagtacgc ggaatgccgg cattcacctg tgccgcctgc cgttgctgcg gcgctgccgc
      181 ctgcggccga ggtgccggcg gcgactgcgg ctgaaccggt ggcatcgtcc cgctgatccg
      241 ggcgcgggca gaactcagca tgacccggta atcgtcatcg acactcggcg cagcgggccg
      301 ttcgaccggt ggtggcgtga tatcgccagc cggcgtcggc acgatcatcg ccatgtcgcg
      361 cgcggccacc gccatgatct cgaccccgcc cggcacaccg cggttggaca ggatgatggc
      421 gtcgttgcca agggtttcct tgacttttct caaggcgtcc cgcgcattgg cggcaatgaa
      481 ttttctgacg ttcatgttct acctccgatg atcgaggtca ccttgatgat tcgattctca
      541 ggaacttcgt tgtgcgacag caccttcagt tgcggtacgg tgcggcgcag gaatctggaa
      601 agcagcaggc gcaggctgga cggcaccagg aggacggccg gcagccccag cccttcctgg
      661 cgccccgcgg cggcggcggt ttcgcgcacc agggtatcgg caaggccggg ctcgaaactg
      721 gtgttttccg agccgccagc gaccgcctgc gccagcaggc gctcgagcgt cggatcaagc
      781 gacatgacct gcatctcgcc gttgcccggg aacagctgct ggacgatggc gcggccgagg
      841 gcgatgcgca cctggacgat gaggtcgtcg gcgctctgcg tgcgcagcgc gtgatcggcg
      901 acggtctcga tgatggtgcg catgtcgcgg atgtgcacgc cttcgtcgag caggccctgc
      961 aggattttct gcaggctgcc cagcggcagg accttgggca ccaggtcctc gaccagcttg
     1021 ggcatttcct tggccagatg gtcgagcagt tgctgcactt cctggcgccc gagcaattcg
     1081 gcggcgtggg tcaggatcag gtggttgagg tgggtggcga cgacggtcga cgcatcgacc
     1141 acggtgtagc cgtaagcctg cgcctggtcg cgctgcatga tgtcgatcca caccgccggc
     1201 agaccaaagg ccgggtcctt ggtcggcgtg ccactcaggg tgccagcgac gcgccccgga
     1261 ttgatcgcca ggaactggcc ggcataggct tcggcggcgc cgacctcgac gcccttcaaa
     1321 aggatgcggt aggcgttcgg cttcaattcg aggttgtcgc ggatgtgcac cggcgagacc
     1381 aggaagccga cttcctgggc gaacttcttg cggatgccgc ggatgcgacg cagcaattcg
     1441 ccgtcctgcc tcttgtccac cagcgggatc aggcgataac cgatctccag accgagcacg
     1501 tcgagcggcg cgacatcgct cc
//
LOCUS       DAOSDX010000810         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27403_length_1522_cov_3.374915, whole genome shotgun sequence.
ACCESSION   DAOSDX010000810 DAOSDX010000000
VERSION     DAOSDX010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27403_length_1522_cov_3.374915"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLN02_14520"
     CDS             complement(<1..1253)
                     /locus_tag="PLN02_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HOI53591.1"
                     /translation="MNRYPLWKYVVVAIALIFGFIYTLPNFFGESPAVQVSSAKVTLK
                     IDAATQARVEDALKAAGIAHDGVQLDNVGVKVRFKDTDTQLKAKDAIERAFNPDRSDP
                     QYVVALNLLSASPQWLTSLHALPMYLGLDLRGGVHFLLQDDMPGVVTKRLDSTAADLR
                     SLLRDKNIRHAGINRDGERIVIKFRDAETRAAASKVLTSNADDLALAEAGQGDDLRLL
                     ATLKPEAQKKIAEFAIKQNINTLHNRINELGVAEPVIQQQGADRIVVQLPGVQDTAKA
                     KDILGRTATLEIRMVDDSTGALEAGLAGNPPFGTELYTERGGSPLLVKKQVVLTGDRL
                     TDAQPGFDGQTQEPAVHLTLDAAGARIFKDVTRDNVGKRMAILLIEKGKGEVVTAPVI
                     RTEIGGGRVQISGRMTTSEANDVALL"
     gene            complement(1302..>1522)
                     /gene="yajC"
                     /locus_tag="PLN02_14525"
     CDS             complement(1302..>1522)
                     /gene="yajC"
                     /locus_tag="PLN02_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOI53592.1"
                     /translation="MIRPQMKKSKEHKALLAALQKGDEVVTGGGIVGRITKVDDNYVT
                     VEIAKDTEVVVQKPAIGIVLPKGTLKSL"
BASE COUNT          216 a          457 c          544 g          305 t
ORIGIN      
        1 agcagggcga cgtcgttggc ttcgctggtg gtcatgcgac cggaaatctg cacccggcca
       61 ccgccgattt cggtacggat gaccggcgcg gtgacgactt cgcccttgcc cttctcgatg
      121 agcaggatgg ccatgcgctt gccgacgttg tcgcgcgtca cgtccttgaa gatgcgggcg
      181 ccggcggcgt cgagcgtcag gtgcacggcc ggttcctggg tctggccgtc gaagccgggc
      241 tgggcgtcgg tcaggcggtc gccggtgagg acgacctgtt tcttgaccag cagcggcgag
      301 ccgccgcgct cggtatagag ctcggtgccg aacggcgggt tgccggcgag accggcttcc
      361 agcgcgcccg tgctgtcgtc gaccatgcgg atttccaggg tcgccgtgcg gcccaggatg
      421 tccttggcct tggccgtgtc ctgcacgccc ggtagctgga cgacgatgcg gtcggcgccc
      481 tgctgctgga tcaccggttc ggcgacgccg agctcattga tccggttgtg cagcgtgttg
      541 atgttctgct tgatcgcgaa ttcggcgatc ttcttctgcg cttccggctt cagcgttgcc
      601 agcaggcgca ggtcgtcgcc ctggccagct tcggccagcg ccagatcgtc ggcgttgctg
      661 gtcagcacct tgctggcggc ggcgcgggtt tcggcgtcgc ggaacttgat gacgatgcgc
      721 tcgccgtcgc ggttgatgcc ggcgtggcgg atgttcttgt cgcgcagcag gctgcgcaga
      781 tcggcggcgg tcgaatcgag gcgcttggtg acgacgcccg gcatgtcgtc ctggagcagg
      841 aagtgcacac cgccgcgcag gtcgaggccg aggtacatcg gcagcgcatg cagcgaggtc
      901 agccattgcg gcgaggcgct cagcaggttt agcgcaacga cgtattgcgg atcgctacga
      961 tccggattga aggcgcgctc gatcgcgtcc ttggccttca actgggtatc ggtgtccttg
     1021 aagcggacct tgacgccgac gttgtcgagc tgcacgccgt catgggcgat gccggcggcc
     1081 ttcagcgcgt cttcaacgcg ggcctgggtg gcggcgtcga tcttgagcgt gaccttggcg
     1141 ctggagacct ggacggccgg cgattcgccg aagaaattag gcagggtgta gatgaagccg
     1201 aagatcagcg caatggcgac gacgacgtac ttccagagag ggtagcggtt catggcggct
     1261 ttacagcgag aagggcggcc gtggccgccc ttcgggatgg attacagcga cttcagcgtg
     1321 cccttgggca ggacgatgcc gatggccggc ttctggacga cgacttcggt gtccttggcg
     1381 atctcgacgg tgacgtaatt gtcgtcaacc ttggtgatac ggccgacgat gccgccgccg
     1441 gtgaccactt cgtcgccctt ctgcagcgcg gcgagcagcg ccttgtgctc cttggacttc
     1501 ttcatctgcg ggcggatcat ca
//
LOCUS       DAOSDX010000811         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27413_length_1522_cov_2.529652, whole genome shotgun sequence.
ACCESSION   DAOSDX010000811 DAOSDX010000000
VERSION     DAOSDX010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27413_length_1522_cov_2.529652"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLN02_14530"
     CDS             <1..429
                     /locus_tag="PLN02_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOI53593.1"
                     /translation="VPPSPPPTISPEPTNASPSAAPAAAAASPLASQQTSQPASQPTS
                     QPTSPPVFNAAYLRNPAPAYPATSRRYGEAGRVLLRVRVLPSGQPDAVVLQQSCGHDR
                     LDAAALDAVRRWRFVPARQGEQAIAAWVVVPIQFSLQEAG"
     gene            495..1453
                     /locus_tag="PLN02_14535"
                     /pseudo
     CDS             495..1453
                     /locus_tag="PLN02_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
BASE COUNT          150 a          612 c          515 g          245 t
ORIGIN      
        1 gtcccgccca gccccccgcc caccatcagc ccggagccga caaacgcatc gccgagcgcc
       61 gcgccggccg cagcagccgc ctccccgctc gcgtcgcagc agacttcgca acccgcatcg
      121 cagcccacct cgcaacccac ctcacccccc gtcttcaacg ccgcctacct gcgcaacccg
      181 gcgcccgcct acccggcgac cagccgccgc tacggcgagg cggggcgcgt gctgctgcgc
      241 gtccgcgtgc tgccttccgg ccagcccgac gcagtcgtgc tgcagcagag ttgcggccac
      301 gaccggctcg acgcggcggc gctcgacgcc gtgcgccgct ggcgtttcgt gccggcccgc
      361 cagggcgagc aggcgatcgc cgcctgggtc gtcgtgccga tccagttcag cctgcaggag
      421 gccggctgat gcgcgccgcc gcgctgccct ggccgcgtct cggccccggc ctgatggcgg
      481 cggtcgccgc ggcgatgctg ttcgccctgt gcatcggccg ctacccgctg ccgctcgccg
      541 acatcctgcg tttcctcggc gccgcgctcg gcctcgtcga actgccggcg cgcgagtacg
      601 acacgctgtt caacctgatc gtccgcatcc gcctgccgcg cgttctcgcc gccgtcctcg
      661 tcggcagcgc gctggccagc gccggcgccg ccttccaggg cgtcttccgc aacccgctgg
      721 tgtcgcccgg gctgctcggc gtgctcgccg gttcggcctt cggcgccgcc ctcggcatcc
      781 tgctcgacct gccctggcgc tggtccaggc gctggccttc gccatgggcg tcgccgcggt
      841 cggcgtcggt gtcggcatcg cgacgctgtt cggcaacggc agcatcatca tgatggtgct
      901 cggcggcgtg atttccggcg cgctgttcac gtcgctgctg tcgatggtca agtacgccgc
      961 cgacccctac aaccagttgc cggccatcgt gcattggctg atgggcagcc tggcgctcgc
     1021 cgatctcggc cagatcggcg tgctggcggt gccgatcggc ttcggcgtgc tcctcctcgg
     1081 caccctcggg cgcggtctcg acgcgctcag catgggcgac gacgaggcgc gcgcgctcgg
     1141 cgtgccggtg cgcgccctgc gcttcggcat cgtcgcgctg gcgacgctga tctcggcact
     1201 gaccgtgtcg ttggccggca tcatcggctg ggtcgggctg atcgtgccgc acatcgcccg
     1261 cctcctggtc ggccccggca acgccgtgct gctgccgacc agcgccgcgc tcggcgcctg
     1321 cttcctgctc gccgccgacg gcctggcgcg caccgtggcg agcaccgaag tgccgatcgg
     1381 catcgtcacc gaactgatcg gcgtcgtcgc cttcgtcctg gttctcggcc gcgtgcgccg
     1441 ggggtggctg tgaacggcgc gcccgtgctc tgcggccagg cgatcgagca cgcctaccag
     1501 gggcgcgtcg tgctggacgg cg
//
LOCUS       DAOSDX010000812         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27416_length_1522_cov_2.346285, whole genome shotgun sequence.
ACCESSION   DAOSDX010000812 DAOSDX010000000
VERSION     DAOSDX010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27416_length_1522_cov_2.346285"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLN02_14540"
     CDS             complement(<1..210)
                     /locus_tag="PLN02_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOI53594.1"
                     /translation="MSLSIAQDMDETTQLFEDVAHYFSLLADPTRLRILFTLCQDERP
                     VNDVVEKIGLTQANISRHLNILFRAG"
     gene            complement(335..>1522)
                     /locus_tag="PLN02_14545"
     CDS             complement(335..>1522)
                     /locus_tag="PLN02_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53595.1"
                     /translation="AADLEQMTAAARAWAARGLIDPVANLARQRLWLFHGSNDGVVKQ
                     PVNAALDQWFAGFTPASQRFIQDSFNAGHAQISAACAGSACQPCARTGGDFINACHSN
                     AEDAPYDAAGGALQMFYGPLARTASEALGARPQAFDQRVHARRAGAAVAPLKISLADQ
                     GYVYVPKDCAAGQACRLHIAFHGCQQQAEAIGEAFVNKAGFNEWADANRIVVLYPQTV
                     AAVAPPQTPLNPQGCWDWWGYNDFGWDMAGHYATAGGDQMASVWSMAERLASGRRAAP
                     SVPSASAPELRVADRADNQIVLAWHIVPGASAYRLLRDGVAVAEIAAAQAPSWVDNGL
                     QPDTAYTYRLLSVDAEGREAVLANALIVQTTTPPAACDPYFSLAQNRPVTRKNRPTRQ
                     ICP"
BASE COUNT          218 a          461 c          597 g          246 t
ORIGIN      
        1 gccggcgcgg aagaggatgt tgaggtggcg cgagatgttg gcctgggtga ggccgatctt
       61 ttcgacgacg tcattgaccg ggcgttcgtc ctggcacagg gtgaacagga tgcgcaggcg
      121 cgtagggtcg gcgagcaggc tgaaatagtg cgcgacgtcc tcgaataatt gtgtggtttc
      181 gtccatgtcc tgtgcgatcg atagcgacac cgcggcgccg ggaatggcgg cgcgactggc
      241 cggggagccg ccggctgatc gcgccttgcg cgcggtgtct gggccgccat tataggggcg
      301 gcgccagcca ggcagcgacc cggctgctcg gcgatcatgg acagatctgg cgcgtcggcc
      361 ggtttttccg ggtcaccggc cggttctgcg ccagggaaaa atagggatca caggcggcgg
      421 gcggcgtggt ggtctggacg atcagggcat tggcgagcac ggcttcgcgg ccctcggcgt
      481 cgacgctgag caggcgatag gtgtaggcgg tatccggctg gaggccgtta tcgacccagg
      541 atggcgcctg cgcggcggcg atttcggcga cggcgacgcc gtcgcgaagc agtcgatagg
      601 cgctggcgcc ggggacgatg tgccaggcga ggacgatctg gttgtcggcg cggtcggcaa
      661 cgcgcaactc gggcgcgctg gcggatggca ccgacggcgc ggcgcggcgg ccactggcca
      721 ggcgctcggc catgctccag acgctggcca tctggtcgcc gccggcggtg gcgtaatggc
      781 cggccatgtc ccagccgaaa tcgttgtagc cccaccagtc ccagcagcct tgcgggttga
      841 gcggggtctg cggcggggcg acggcggcaa ccgtctgcgg gtagaggacg acgatccggt
      901 tggcgtcggc ccattcgttg aagccggcct tgttcacgaa ggcctcgccg atcgcctcgg
      961 cctgctgctg gcaaccgtgg aaggcgatgt gcaggcggca ggcctggccg gcggcgcagt
     1021 ccttcggcac gtaaacgtaa ccctgatcgg ccagcgaaat cttcagcggg gcgacggcgg
     1081 cgccggcgcg ccgggcatgc acgcgctgat cgaaggcttg cggcctggcg ccaagagctt
     1141 cgctggcggt ccgcgccagc ggcccgtaaa acatctgcag cgcgccgccg gcggcgtcgt
     1201 agggcgcgtc ttcggcgttg ctgtggcagg cgttgatgaa atcgccgccg gtcctggcgc
     1261 agggttggca ggcgctgccg gcgcaggcgg cgctgatctg ggcgtgaccg gcgttgaagc
     1321 tgtcctggat gaagcgctgg ctggccggag tgaagccggc gaaccactgg tcgagcgcag
     1381 cgttgaccgg ctgtttgacg acgccgtcgt tgctgccatg gaacagccag agacgttgcc
     1441 gcgccagatt ggcaaccggg tcgatcagcc ctctggccgc ccaggcgcgg gcggcggcgg
     1501 tcatctgttc gaggtcggcc gc
//
LOCUS       DAOSDX010000813         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27492_length_1519_cov_3.906421, whole genome shotgun sequence.
ACCESSION   DAOSDX010000813 DAOSDX010000000
VERSION     DAOSDX010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27492_length_1519_cov_3.906421"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLN02_14550"
     CDS             complement(<1..912)
                     /locus_tag="PLN02_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOI53596.1"
                     /translation="MESFAQLFEESLARQEMRQGEVITAEVVLIDHNFVVVNAGLKSE
                     SYVPVEEFLNDQGEVEVAVGDFVQVAIEMLEDGYGSTRLSRDRAKRIASWNFLEQALN
                     DGALVSGTITGKVKGGLTVMSNGVRAFLPGSLVDMRPVKDTTPYEGKTMEFKVIKLDR
                     KRNNVVLSRRAVLEATADKDREKLMENLKEGATVKGIVKNITDYGAFVDLGGIDGLLH
                     ITDLAWRRVRHPSEVLNVGDEVTAKILKFDAEKNRVSLGMKQLGDDPWVGIARRYPAG
                     TRLFGKVTNLTDYGAFVEIEQGIEGLVH"
     gene            complement(1004..>1519)
                     /gene="cmk"
                     /locus_tag="PLN02_14555"
     CDS             complement(1004..>1519)
                     /gene="cmk"
                     /locus_tag="PLN02_14555"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOI53597.1"
                     /translation="NVAWDDESGVAAIAAGLDVVFAEDDIRLAGAPVGDAIRTEEISA
                     GASKVAALPAVRAALLFRQRAFNTAPGLVGDGRDMGSVVFPRAPLKVFLTATAEARAE
                     RRHKQLIEKGFSANLPDLLADLQLRDARDSQRSVAPLRQEVDARLLETTHLTNEQAVK
                     QVLDWYAEAAK"
BASE COUNT          282 a          451 c          488 g          298 t
ORIGIN      
        1 gtgcaccagg ccttcgatgc cttgttcgat ctcgacgaaa gcaccgtagt cggtcagatt
       61 ggtgaccttg ccgaagaggc gggtgccggc cgggtaacgg cgggcgatgc cgacccacgg
      121 atcgtcgccg agctgcttca tgcccagcga gacgcggttc ttctcggcgt cgaacttgag
      181 gatcttggcg gtaacttcgt caccgacatt gagcacttcg ctcgggtgac ggacacggcg
      241 ccaggccagg tcggtgatgt gcagcaggcc atcgatgccg cccaggtcga cgaatgcgcc
      301 gtagtcggtg atgttcttga cgataccctt gacggtggca ccttccttga ggttttccat
      361 cagcttttcg cgatccttgt cggcggtcgc ttcgaggacg gcacggcggg acaggacgac
      421 gttgttgcgc ttgcggtcaa gcttgatgac cttgaactcc atggtcttgc cttcgtacgg
      481 cgtggtgtcc ttgaccggac gcatgtcgac cagcgagccc ggaaggaagg cacggacgcc
      541 gttggacatg acggtgagac cacccttgac cttgccggtg atggtgccgg aaaccagggc
      601 accgtcgttg agggcttgct cgaggaagtt ccaggaggcg atgcgcttgg cgcgatcgcg
      661 ggaaagacgg gtcgaaccgt agccgtcttc cagcatttcg atggcgacct ggacgaaatc
      721 gccgacggcg acttcaactt caccctggtc gttgaggaat tcttctacgg gaacgtagga
      781 ttcggatttc aggccggcat tgaccacgac gaaattgtgg tcgatgagca ccacttctgc
      841 agtgatgact tcgccttgac gcatttcctg gcgagccagg gattcttcaa aaagttgagc
      901 aaaagattcc attacggagc gagttccttg ccgcgagcca agcggcccac agcgggttga
      961 ggtgaaaaac aagtcgccga ccggaatgac cggcaacacc gtgctacttg gctgcttccg
     1021 cgtaccagtc cagcacctgt ttcaccgcct gttcgttggt gagatgcgtg gtttccagca
     1081 gcctggcatc cacttcctgc ctgagcggcg ccacgctgcg ctgcgaatct cgcgcgtcac
     1141 gtagctgcag gtcagccaga aggtcgggga gattagcaga gaaacctttt tcgatcaact
     1201 gcttatgacg acgttcggcg cgcgcttcgg cggtggcggt aagaaagacc ttgagtggcg
     1261 ctcgcgggaa gaccaccgag cccatgtcgc ggccgtcgcc gaccaggccg ggcgccgtgt
     1321 tgaaggcgcg ctgacggaac agcagagccg cgcgcacggc cggcagggcc gcgactttcg
     1381 aggcaccggc cgagatttcc tcggtacgga tggcatcgcc gaccggcgcg ccggccaggc
     1441 ggatgtcatc ctcggcaaac accacgtcga gcccggcagc gatcgccgcg acaccgcttt
     1501 cgtcatccca ggccacgtt
//
LOCUS       DAOSDX010000814         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27593_length_1515_cov_3.252740, whole genome shotgun sequence.
ACCESSION   DAOSDX010000814 DAOSDX010000000
VERSION     DAOSDX010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27593_length_1515_cov_3.252740"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLN02_14560"
     CDS             <1..199
                     /locus_tag="PLN02_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOI53598.1"
                     /translation="AHDAGELWPRQAFLKRAGTATISIGPAFDASGMSDAEVNQRVED
                     WIEAEMRRLSPHRYPDAGKAD"
     gene            180..896
                     /locus_tag="PLN02_14565"
     CDS             180..896
                     /locus_tag="PLN02_14565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOI53599.1"
                     /translation="MPAKPTETSRRIVVAGQTLDYTLRRSARRSIGLSIDQRGLRVGA
                     PQGAALRDIEALIRQHGDWVLDKLAVWRERPAATRFAVVDGAVLDLLGSPTRLRVDPA
                     KRAGARFATGEIHLAVPAATDPARVLEAALRERARALFAERLAHFAPQLGVAAPPLRL
                     SSARTRWGSCNARGNIALNWRLLLLPPAIVDYVVCHELAHLKEMNHSPRFWSVVEQLC
                     PDWKALRNELRHLGRQLPQF"
     gene            908..1288
                     /gene="gloA"
                     /locus_tag="PLN02_14570"
     CDS             908..1288
                     /gene="gloA"
                     /locus_tag="PLN02_14570"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HOI53600.1"
                     /translation="MRILHTMLRVVDLDRSIAFYTEILGMQLLRRNDYPEGRFTLAFV
                     GYGSEDSEAVLELTHNWDTASYELGNAYGHIALAVPDAAAACAAIKARGGKVVREAGP
                     MKHGSTIIAFVEDPDGYKVELIQR"
     gene            complement(1296..>1515)
                     /locus_tag="PLN02_14575"
     CDS             complement(1296..>1515)
                     /locus_tag="PLN02_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014237699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HOI53601.1"
                     /translation="EVTGITMTPDGRHIFVGIQHPGDEAPADDPTRYSGWPASQWPRR
                     ADGLALPAGRPRSGVVVISREDGGIVGS"
BASE COUNT          235 a          581 c          493 g          206 t
ORIGIN      
        1 cgcccacgac gccggcgaac tgtggccgcg ccaggctttc ctgaagcgcg ccggcaccgc
       61 gaccatcagc atcggcccgg ccttcgacgc cagcggcatg agcgatgcgg aagtcaatca
      121 gcgggtcgag gactggatcg aagccgaaat gcgccgcctc tcgccgcacc gttacccgga
      181 tgccggcaaa gccgactgag acgtcccggc gcatcgtcgt cgccggccag acgctcgact
      241 acacgctgcg ccgcagcgcc cggcgcagca tcggcctgag catcgaccag cgcggcctgc
      301 gcgtcggcgc cccgcaaggc gccgcgctgc gcgacatcga ggcgctgatc cgccagcacg
      361 gcgactgggt actcgacaag ctcgccgtct ggcgcgagcg cccggcggcg acccgcttcg
      421 ccgtcgtcga cggcgccgtg ctcgacctcc tgggcagccc gacccgcctg cgcgtcgatc
      481 cggcaaagcg cgccggcgcc cgtttcgcca ccggcgaaat acacctcgcc gtgcccgccg
      541 ccaccgaccc cgcccgggtg ctcgaagcgg cgctacgcga gcgggcgcgc gcgctgttcg
      601 ccgaacgcct ggcccatttc gcgccacagc tcggcgtcgc ggcgccgccc ttgcgtctgt
      661 cgtcggcgcg cacgcgctgg ggcagttgca acgcgcgcgg caacatcgcg ctcaactggc
      721 ggctgctcct gctgccgccg gccatcgtcg attacgtcgt ctgccacgaa ctggcgcacc
      781 tgaaggaaat gaaccacagc ccgcgtttct ggtctgttgt cgaacaactc tgcccggact
      841 ggaaggcgct gcgcaacgaa ctgcgccacc tcggccggca actgccgcaa ttctgaaagg
      901 acaccccatg cgcatcctgc acaccatgct ccgcgtcgtc gatctcgacc gctcgatcgc
      961 cttctacacc gaaatcctcg gcatgcagtt gctgcgccgc aacgactacc cggaaggccg
     1021 cttcacgctg gccttcgtcg gctacggcag cgaagacagc gaagccgtgc tcgaactgac
     1081 gcacaactgg gacaccgcca gctacgagct gggcaacgcc tacggccaca tcgcccttgc
     1141 cgttcccgac gccgctgccg cctgcgcggc gatcaaggcg cgcggcggca aggtggtgcg
     1201 cgaagccggg ccgatgaagc acggcagcac gatcatcgcc ttcgtcgagg accccgacgg
     1261 ctacaaggtc gaactgatcc agcgctgacg ccgcgtcagc tgccgacgat ccccccgtcc
     1321 tcccggctga tcacgacgac gccgctgcgc ggccggccgg cgggcagggc gaggccgtcg
     1381 gcgcgccgcg gccactggct ggccggccag cccgagtagc gggtgggatc gtcggcgggc
     1441 gcctcgtcgc ccgggtgctg gatgccgacg aagatgtggc gaccatccgg cgtcatcgtg
     1501 atgccggtca cttcg
//
LOCUS       DAOSDX010000815         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27674_length_1511_cov_3.477335, whole genome shotgun sequence.
ACCESSION   DAOSDX010000815 DAOSDX010000000
VERSION     DAOSDX010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27674_length_1511_cov_3.477335"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1511)
                     /locus_tag="PLN02_14580"
     CDS             complement(<1..>1511)
                     /locus_tag="PLN02_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOI53602.1"
                     /translation="NGREYLIRHIGRSASLEDLRNLALTARNGQAILLRQVAEVNFAA
                     AIKRGDAGFNGEAAVILSIQKQPTADTVDLTRRLESALADMEKSLPAGVLAPQVIFRQ
                     ADFIQASIDNLQAKLLLAACLVAAVLFFFLGNLRTMLISLTAIPLSILIAVLVFKWLG
                     LSINTMTLGGLAIAIGELVDDAVVDVENILRRLRGAAARGAPFKLTELVVAASLEVRS
                     AIVYATLIIALVFVPLFALPGIEGRLFVPLGIAYIVAILASLLVSVTVTPVLSWYLLP
                     KLAATRHGDTRLVEWLKRHYRRALEAVLRAPRRPVALAAAAVALAAAAVPFFPTTFLP
                     PFNEGSMTVTLRLNPGITLAESVRVASQAERLLLGVPEVEHIGRRSGRAELDEHAEGV
                     YVSELEIRLKPGGRNKEALYADIRQRLKDLPGSLNLGQPISHRIDHMLSGVRAQVAIK
                     VFGDDLDVLRAQAAALQQRLQQIPGVADLEIEKQVLAPQIKVHVDFAAAARYGL"
BASE COUNT          255 a          495 c          535 g          226 t
ORIGIN      
        1 agcccgtaac gcgcggcggc ggcgaaatcg acatgcacct tgatctgcgg cgccagcacc
       61 tgcttttcga tctccagatc ggcaacgccg ggaatctgct gcaggcgctg ctgtagcgcc
      121 gccgcctggg cgcgcaggac gtcgagatcg tcgccgaaga ccttgatcgc cacctgggcg
      181 cgcaccccgg acagcatgtg gtcgatgcgg tgcgagatcg gctggccgag gttgaggctg
      241 cccggcaggt ccttcaggcg ttgccggatg tcggcgtaga gcgcttcctt gttgcgcccg
      301 ccgggcttga ggcggatttc cagttccgag acgtaaacgc cctcggcatg ctcgtcgagt
      361 tcggcccgcc cggagcggcg gccgatgtgc tcgacctccg gcacgccgag cagcaggcgt
      421 tcggcctggc tggcaacgcg taccgattcg gccagcgtga tgcccggatt caggcgcagc
      481 gtcaccgtca tcgacccttc gttgaacggc ggcagaaagg tggtcgggaa gaagggcacg
      541 gcggcggcgg cgagcgcgac ggctgcagcg gccagtgcca ccggccggcg cggtgcccgc
      601 agcaccgctt cgagcgcccg ccggtagtgg cgcttgagcc attcgaccag gcgcgtatcg
      661 ccgtgccggg tggccgccag tttcggcagc agataccacg agagcaccgg cgtcaccgtc
      721 accgagacca gcaaggaggc caggatcgcg acgatgtagg cgatgccgag cggcacgaac
      781 agccgcccct cgatccccgg cagcgcgaac agcggcacga agaccagggc gatgatcagc
      841 gtcgcgtaaa cgatcgccga gcgcacttcg agcgaggcag cgacgaccag ttcggtcagc
      901 ttgaacggcg cgccgcgcgc cgccgcaccg cgcaggcggc gcaggatgtt ctcgacgtcg
      961 acgacggcgt cgtccaccag ttcgccgatg gcgatcgcca gcccgcccag cgtcatcgtg
     1021 ttgatcgaca ggccgagcca cttgaagacc agcaccgcga tcaggatgga aagcgggatc
     1081 gcggtcaggg agatcagcat cgtgcgcagg ttgccgagga agaagaacag caccgcggcg
     1141 accaggcagg cggcgagcag cagcttggcc tgcaggttgt cgatcgacgc ctggatgaaa
     1201 tcggcctggc ggaagatcac ctgcggcgcc agcacgccgg ccggcaggct tttctccatg
     1261 tcggccaggg cactttccag gcgtcgggtg aggtcgaccg tatcggccgt gggctgcttc
     1321 tggatgctca ggatgaccgc tgcctcgcca ttgaaaccgg cgtcgccgcg cttgatggcg
     1381 gcggcgaaat tgacctcggc gacctggcgg agcagtatcg cctgcccgtt gcgcgcggtc
     1441 agggccaggt tgcgcaagtc ttccaggctg gcactgcggc cgatgtggcg aatcaggtac
     1501 tcgcgcccgt t
//
LOCUS       DAOSDX010000816         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27851_length_1504_cov_2.828157, whole genome shotgun sequence.
ACCESSION   DAOSDX010000816 DAOSDX010000000
VERSION     DAOSDX010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27851_length_1504_cov_2.828157"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLN02_14585"
     CDS             complement(<1..228)
                     /locus_tag="PLN02_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53603.1"
                     /translation="MRLLPALRPGPPAWSLLLLALALLAHAPALPLAAWPALLLALLA
                     RRLPSGHIAASLRLIGLLAVYTLAALIFGWFD"
     gene            complement(233..616)
                     /locus_tag="PLN02_14590"
     CDS             complement(233..616)
                     /locus_tag="PLN02_14590"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOI53604.1"
                     /translation="MNSAKKTSSGLLVTVAWLLPLLTAGATGYLVLRDHPDDEAARIA
                     RARDDIRALSGALLADPTLPATADGLAALVADGRLPHIPDDPWGRPYRYLNPGELHAW
                     ELYSLGPDGVVSQDDVVNWNLYGGR"
     gene            complement(613..>1504)
                     /locus_tag="PLN02_14595"
     CDS             complement(613..>1504)
                     /locus_tag="PLN02_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53605.1"
                     /translation="ACHTEIRPTTVAGEEPMNLQETYDEWRHSPWAKKNVHCQDCHMA
                     ADPAATVAALKRGEKKPRGVSHRIVGNNYLLANPDLPGKLMTTLRAGAPTGLNKMFKR
                     DEYEAEQRKTSEQVVGLLRQAADLSVQANPAQLTVTVTNSGAGHALPTGPLDQRHLWL
                     EVKVSDAAGKTHFHSGAFDAQSGRTDPDAAMWVKEVTGPDGKRALHHLLFDARHLHYP
                     RKPIPAGASDRVDYPLPTLPAGHYRVEVKLWYRLAFQEILDNFTSQTGSKPDVIIPPL
                     AIASTQGELTISAQTAEAGR"
BASE COUNT          216 a          458 c          566 g          264 t
ORIGIN      
        1 gtcgaaccag ccgaaaatca gtgctgcgag cgtgtagacc gcaagcaggc cgatcaggcg
       61 caggctggcg gcaatgtgcc cgctcggcag acgacgcgcc agcaacgcca gcaggagcgc
      121 cggccaggct gccagcggca gcgccggggc gtgcgcgagc agcgccagcg ccagcagcag
      181 caaggaccag gccggcgggc cggggcgcaa ggcgggaagc aaacgcatcg tctcagcggc
      241 cgccgtagag gttccagttc accacgtcgt cctggctgac gacgccgtcc ggaccgagcg
      301 aatagagttc ccaggcatgc agttcgccgg ggttcaggta gcggtagggg cggccccagg
      361 gatcgtccgg aatgtgcggc aggcggccgt cggcgaccag tgcggcgagg ccgtcggcgg
      421 tggcaggtag cgtcggatcg gcaagcagcg ctccggaaag ggcgcggatg tcgtcgcggg
      481 cgcgggcgat ccgggcggct tcatcgtccg gatggtcgcg cagtaccaga tagccagtgg
      541 cgcccgccgt gagcaaaggc agcagccagg caacggtgac gagcaggccg gagctggtct
      601 ttttggctga attcatcgtc cggcctcagc cgtctgcgcg cttatcgtca gttcgccctg
      661 ggtgctggcg atggcgagcg gcggaatgat cacgtccggc ttgctgccgg tctggctggt
      721 gaagttgtcg aggatttcct ggaaggcgag ccggtaccag agcttgacct cgacccggta
      781 atgtccggcg ggcagcgtcg gcagcgggta gtcgacgcgg tcgctggcgc cggccgggat
      841 cggcttgcgc gggtaatgca ggtggcgggc gtcgaagagc aggtggtgca gcgcccgttt
      901 gccgtccggt ccggtcactt ccttgaccca cattgccgcg tcggggtcgg tgcgcccgct
      961 ctgcgcgtcg aaggcaccgc tgtggaaatg cgtcttgccg gcagcgtcgc tgaccttgac
     1021 ttccagccac aggtggcgct ggtcgagcgg tccggtcggc aaggcatggc cggcgccgct
     1081 gttggttacg gtcaccgtca attgcgccgg attggcctgg acgctgaggt ccgctgcctg
     1141 acgcagcagg ccgactacct gttcgctggt cttgcgctgt tcggcttcgt actcgtcgcg
     1201 cttgaacatc ttgttgaggc cggtcggggc gcccgcgcgc agggttgtca tcagcttgcc
     1261 gggcaggtcc gggttggcga gcaggtagtt gttgccgacg atgcggtgcg agacgccgcg
     1321 cggcttcttc tcgccgcgtt tgagcgcggc aacggtggcc gcagggtcgg ccgccatgtg
     1381 gcagtcctgg caatggacgt tcttcttcgc ccacggtgaa tgtcgccatt cgtcgtaggt
     1441 ttcctgcagg ttcatcggct cttcgccggc tacggtagtc ggccggattt ccgtgtggca
     1501 ggcg
//
LOCUS       DAOSDX010000817         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27887_length_1502_cov_2.905321, whole genome shotgun sequence.
ACCESSION   DAOSDX010000817 DAOSDX010000000
VERSION     DAOSDX010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27887_length_1502_cov_2.905321"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLN02_14600"
     CDS             <1..380
                     /locus_tag="PLN02_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOI53606.1"
                     /translation="AHIHAGVRDVSVVLQDILNTRLLRAAVAAVPARQLDQLVGRRER
                     ANAEHQQHLASLRRQLQRALEDAFQLVELGGVLAKSAKIEAAYGQGFAGALTQVSGEF
                     DGVVEEIRNSLQALQRSDFFKNN"
     gene            396..1136
                     /locus_tag="PLN02_14605"
     CDS             396..1136
                     /locus_tag="PLN02_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOI53607.1"
                     /translation="MKKTSTIFQDGDHKWVAIVRDPERPGHLIDTNEYLVSHGGQGML
                     LDPGGAEIFPAVFSALAGEFDPSALTAIFASHQDPDICSSLALWLEFNPGMRCYVSWL
                     WAGFIPHFGGAPETFVPIPDAGMEISLGGLPLQAVPAHYLHASGNFHLYDRKAKILFS
                     GDVGAALLPAGQDGLFVEDFDAHIRYAEAFHQRWMGSNEAKRRWCERVAGLDIDFLCP
                     QHGAIYRGADVQRFIHWFDALPVGVIGG"
     gene            1271..>1502
                     /locus_tag="PLN02_14610"
     CDS             1271..>1502
                     /locus_tag="PLN02_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI53608.1"
                     /translation="MATASAEKVKRQYNRWVATESIEDYALRYSPASFRKWSPSVIGN
                     TMIGTNSALSYEAIGALLLLDFGFANAIWALIF"
BASE COUNT          248 a          493 c          477 g          284 t
ORIGIN      
        1 ccgcacacat ccacgccggc gtgcgcgacg tctcggtcgt cctccaggac atcctcaaca
       61 cccggctgct gcgcgccgcc gtcgccgctg ttccggcgcg ccagctggat cagctggtgg
      121 gccggcgcga acgggccaac gccgaacacc agcagcacct ggcgagcttg cgccgacagt
      181 tgcagcgggc gctcgaagac gctttccagt tggtcgagct gggcggcgtg ctggccaagt
      241 cggcgaagat cgaggcggct tacggtcagg ggtttgccgg ggcgctgact caggtttccg
      301 gcgaattcga cggcgtcgtc gaggaaatac gcaattcgct gcaggcgctg cagcgttcgg
      361 attttttcaa gaacaattaa gacagcgggg gcggggtgaa gaaaaccagt accattttcc
      421 aggatggtga ccacaaatgg gtggccatcg tgcgcgatcc ggagcggccg gggcatctga
      481 tcgacaccaa cgaatacctg gtcagccacg gtggccaggg catgctgctc gatcccggtg
      541 gcgccgaaat ctttcccgcc gtcttctcgg cgctcgccgg cgaattcgat ccttccgcgc
      601 tgaccgccat cttcgcctcg caccaggacc ccgatatctg ttcgtcgctg gcgctgtggc
      661 tggaattcaa ccccggcatg cgctgctacg tcagttggct gtgggctggc ttcatcccgc
      721 acttcggcgg tgcgccggag accttcgtgc cgattcccga cgccggcatg gaaatatccc
      781 tcggcggcct gccgctgcag gcggttcccg cccattacct gcatgcctcc ggcaatttcc
      841 acctctacga ccgcaaggcg aagatcctgt tctccggcga cgtcggtgcg gcgctcctgc
      901 cggccggcca ggacgggctc ttcgtcgagg atttcgatgc ccatatccgc tacgccgaag
      961 cctttcacca gcgctggatg gggtccaacg aggccaagcg ccgctggtgc gaacgcgtcg
     1021 ccgggctcga catcgacttc ctctgcccgc agcacggcgc catctaccgc ggcgccgacg
     1081 tgcagcgctt catccactgg ttcgacgcct tgccggtcgg cgtgatcggc ggctgagtcc
     1141 tggctcaccg ctggcgtcgc ccgcagaaat tgctgcgggc attcttcatt cgatgcgtaa
     1201 atccgtctaa catggcgtaa cgcaagcgca tcgaccggag atgcgccgcc agacatgggg
     1261 gtagggcgga atggcgacgg catcggctga aaaggtcaag cgtcaataca atcgctgggt
     1321 ggcgacggag tcgatcgagg actacgcgct acgctattcg ccggcgagtt ttcgcaagtg
     1381 gtcgccgtcg gtgatcggca acaccatgat cggcaccaac tcggcgcttt cctacgaggc
     1441 gatcggtgcc ctgctgctgc tcgatttcgg cttcgccaac gcgatctggg cgctgatctt
     1501 cg
//
LOCUS       DAOSDX010000818         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27893_length_1502_cov_2.350380, whole genome shotgun sequence.
ACCESSION   DAOSDX010000818 DAOSDX010000000
VERSION     DAOSDX010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27893_length_1502_cov_2.350380"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="PLN02_14615"
     CDS             <1..1354
                     /locus_tag="PLN02_14615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HOI53609.1"
                     /translation="SICLIANTAPVAWGAIGIPIVVGGQVAGIPDLALSQMVGRTLPF
                     LSFLVPLYLVILMAGWKKGPEVWPACLVSGGSFAIAQYFSANSPLTAPLLPDLIASIS
                     SIVCTVAFLKFWHPKESWHFADEPKREGKAKLLYTGGQIFRAWAPFIILSLFVCAWGI
                     KPVNQALDQITKTVFNFAFPIAGLDQMVIDQAGKAKAAVYKFNVLSAAGTAILFAWFF
                     SIVVMGASLKTAGGVARDTLVTLKWPIVTIATILGFAYIMNFSGMAITLGYAVATTGV
                     AFPFFAALLGWLGVFMTGSDTSTNALFGKLQAVTAEKIGIDPVITMSANTCGGVCGKM
                     ISPQSISVATGATGMVGRESEIFRFTFKHSIGMAVVVGGMHMLWAYVFPGMVPTYVKP
                     VAAAAAAAAGVVQAAINPVGLMWLGIFVAIITCVTFLARRLGADLEAPAAGHDEVHFH
                     "
BASE COUNT          219 a          552 c          440 g          291 t
ORIGIN      
        1 gtcgatctgc ctgatcgcca acacggcgcc ggtcgcctgg ggcgccatcg gcatccccat
       61 cgtcgtcggc ggccaggtgg ccggcattcc cgatctggcg ctcagccaga tggtcggccg
      121 caccttgccc ttcctcagct tcctggtgcc gctctacctg gtcatcctga tggccggctg
      181 gaagaaaggc ccggaagtct ggccggcttg tctggtcagc ggcggttcct tcgccatcgc
      241 ccagtacttc tcggccaact cgccgctcac cgcgccgctg ctgcccgacc tgatcgcctc
      301 gatctcctcg atcgtctgca cggtcgcctt cctcaagttc tggcacccga aggaaagctg
      361 gcacttcgcc gacgagccga agcgcgaagg caaggccaag ctgctttata ccggcgggca
      421 gatcttccgc gcctgggcgc cgttcatcat cctctcgctg ttcgtctgcg cctggggcat
      481 caagccggtc aatcaggcgc tcgaccagat caccaagacg gtattcaatt tcgccttccc
      541 gatcgccggt ctcgaccaga tggtgatcga ccaggccggc aaggcgaagg ccgcggtgta
      601 caagttcaac gtgctgtcgg ctgccggcac ggcgatcctc ttcgcctggt tcttctccat
      661 cgtcgtcatg ggcgcctcgc tgaagaccgc cggcggcgtc gcccgcgaca cgctggtcac
      721 gctgaaatgg ccgatcgtca ccatcgccac catcctcggc ttcgcctaca tcatgaactt
      781 ctccggcatg gcgatcacgc tcggctacgc cgttgccacg accggcgtcg ccttcccgtt
      841 cttcgccgcg ctgctcggct ggctgggcgt cttcatgacc ggttccgaca cctcgaccaa
      901 cgcgctgttc ggcaagctgc aggcggtcac cgccgagaag atcggcatcg acccggtgat
      961 cacgatgtcc gccaacacct gcggcggcgt ctgcggcaag atgatctcgc cgcagtcgat
     1021 ctcggtcgcc accggcgcca ccggcatggt cggccgtgaa tcggaaatct tccgcttcac
     1081 cttcaagcac tcgatcggca tggccgttgt cgtcggcggc atgcacatgc tgtgggccta
     1141 cgtcttcccg ggcatggtgc cgacctacgt caagccggtc gcggcggccg ccgctgctgc
     1201 cgccggtgtc gtgcaggcgg cgatcaaccc ggtcggcctg atgtggctgg ggatcttcgt
     1261 cgccatcatc acctgcgtca ccttcctggc ccgccgcctc ggcgccgacc tcgaagcgcc
     1321 cgccgccggc cacgacgaag tgcatttcca ctaagcaaga ccgccgccgg ggcccgcccc
     1381 ggcggcattg tccgcccatg agcggcggac aactggctac aatcgccgct gtttcgtttt
     1441 cttctccccg ggaatttcca tgtctgactc cgcgtccgat ccgcgcaagg acgaacccct
     1501 gc
//
LOCUS       DAOSDX010000819         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Azonexus sp. isolate AMR_MDS_1820
            NODE_27936_length_1500_cov_3.001384, whole genome shotgun sequence.
ACCESSION   DAOSDX010000819 DAOSDX010000000
VERSION     DAOSDX010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863349
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Azonexus sp. (wastewater metagenome)
  ORGANISM  Azonexus sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales;
            Azonexaceae; Azonexus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,925
            CDSs (total)                      :: 2,908
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 19 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 3 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Azonexus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27936_length_1500_cov_3.001384"
                     /isolate="AMR_MDS_1820"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1872668"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLN02_14620"
     CDS             complement(<1..873)
                     /locus_tag="PLN02_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOI53610.1"
                     /translation="MNGKPPLPWKRSLLWLLFLGPFFFLSYGQANQLAASREAVASLY
                     FAWERQLPFLPWTIVPYWSIDLLYGLSFLCCRDARAVDRHGQRLLAAQVIAVACFLAF
                     PLRFAFARPATDGLAGAMFTALSAFDQPYNQAPSLHIALLVLIWFQFAKLPLARPWRL
                     LVHGWALLIGISVLTTWQHHFIDVPTGAALGLFCLWLLPEGGLRSPLRRAADAGHLRL
                     AALYAGAAVALLASAIGLGGLALILAWPALSLLLVGWNYAWAGSAGFQKQAGGHSLAA
                     RWLFAPYQFAAWLNS"
     gene            complement(870..>1500)
                     /locus_tag="PLN02_14625"
     CDS             complement(870..>1500)
                     /locus_tag="PLN02_14625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HOI53611.1"
                     /translation="DAIGWRGIRQRKLNIEELLRAALSRLQAEGQPLRILDIAAGHGR
                     YVLEALADAPRPESVLLRDFSPLNVDQGSALIAAKGLSDIARFERGDAFDRASVAAVT
                     PAPTLGIVSGLYELFPDNDRVAASLAGMADAIAPGGYLIYTGQPWHPQLELIARALTS
                     HRDGEAWVMRRRTQAEMDQLVSDAGFEKIEQRIDAWGIFTVSLARRRPA"
BASE COUNT          255 a          477 c          588 g          180 t
ORIGIN      
        1 cgaattgagc caggcggcaa actggtaggg tgcgaacagc cagcgcgcgg cgaggctgtg
       61 gccgccggcc tgcttctgga agccggcgct gcccgcccag gcgtagttcc agccgaccag
      121 cagcagcgac agcgccggcc aggcgaggat cagcgccaga ccgccgaggc cgatggcgct
      181 cgccagcagg gccaccgcag cgccggcata gagcgctgcc agccgcaagt ggccggcatc
      241 ggcggcgcgc cgcagcggcg agcgcagacc gccttccggc agcagccaga ggcagaagag
      301 gccgagcgcg gcgccggtcg ggacgtcgat gaaatggtgc tgccaggtgg tcagcacgga
      361 aatcccgatg agcagcgccc agccgtgcac cagcaggcgc caggggcggg ccagcggcaa
      421 cttcgcgaac tggaaccaga tcaggacgag cagggcgata tgcagcgacg gcgcctggtt
      481 gtacggctgg tcgaaggcgc tcagggcggt gaacatggcg cccgccaggc cgtcggtcgc
      541 tgggcgggcg aaggcgaaac gcagcgggaa ggcgagaaag caggcgacgg cgatcacttg
      601 cgcggcgagc aggcgctggc cgtgccggtc gacggcgcgg gcgtcgcggc agcagaggaa
      661 ggaaagcccg tagaggaggt cgatcgacca atagggaacg atggtccagg gcagaaaggg
      721 tagctgccgc tcccaggcga aatacaggct ggccaccgcc tcgcggctgg ccgccagctg
      781 gttcgcctgg ccgtagctga gaaagaagaa ggggccgagg aagagcagcc agagcaggct
      841 gcgcttccag ggcaatggcg gcttgccgtt catgccgggc ggcggcgggc cagcgagacg
      901 gtgaagatgc cccaggcgtc gatgcgctgc tcgatcttct cgaagccggc gtcgctgacc
      961 aactggtcca tttccgcctg ggtgcggcgg cgcatcaccc aggcctcgcc gtcgcggtgg
     1021 ctggtcagcg cgcgggcgat cagttcgagc tgcggatgcc agggctggcc ggtgtagatc
     1081 aggtagccgc ccggcgcgat ggcgtcggcc atgccggcca gcgaggcagc gacccgatcg
     1141 ttgtcgggga acagctcgta gagtccggag acgatgccga gcgtcggcgc cggcgtcacc
     1201 gccgccaccg aggcgcggtc gaaggcgtcg ccgcgctcga agcgggcgat gtcgctcagg
     1261 cccttggcgg cgatcagggc gctgccctgg tccacgttga gcgggctgaa atcgcgcagc
     1321 aagacggatt ccgggcgcgg cgcgtcggcc agcgcttcca gcacgtagcg gccgtggccg
     1381 gcggcgatgt cgaggatgcg cagcggctgg ccctccgcct gcaggcggct gagcgctgcg
     1441 cgcagcagtt cttcgatgtt gagcttgcgc tggcggatgc cgcgccagcc gatggcgtcg
//
